愛媛大学プロテオサイエンスセンター
膜タンパク質関連論文リスト

Characterization of mitochondrial carrier proteins of malaria parasite Plasmodium
falciparum based on in vitro translation and reconstitution.
Nozawa A, Ito D, Ibrahim M, Santos HJ, Tsuboi T, Tozawa Y.
Parasitol Int. 2020 79:102160.

Substrate specificity of plastid phosphate transporters in a non-photosynthetic diatomand its implication in evolution of red alga-derived complex plastids.
Moog D, Nozawa A, Tozawa Y, Kamikawa R.
Sci Rep. 2020 10:1167.

Novel lineage-specific transmembrane β-barrel proteins in the endoplasmic reticulum ofEntamoeba histolytica.
Santos HJ, Imai K, Makiuchi T, Tomii K, Horton P, Nozawa A, Okada K, Tozawa Y,Nozaki T.
FEBS J. 2019 286:3416-3432.

Engineered membrane protein antigens successfully induce antibodies against
extracellular regions of claudin-5.
Hashimoto Y, Zhou W, Hamauchi K, Shirakura K, Doi T, Yagi K, Sawasaki T, Okada
Y, Kondoh M, Takeda H.
Sci Rep. 2018 8:8383.

Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The PharmacologicalAnalysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ
Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes.
Suzuki Y, Ogasawara T, Tanaka Y, Takeda H, Sawasaki T, Mogi M, Liu S, Maeyama K.
Front Pharmacol. 2018 9:38.

AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against
Human Dopamine Receptor D1 for Protein Analysis.
Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T,Takahashi H, Sawasaki T.
PLoS One. 2016 11: e0156716.

Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR
antigen and biotinylated liposome-based interaction assay.
Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M,Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T.
Sci Rep. 2015 5:11333.

Evidence that the Entamoeba histolytica Mitochondrial Carrier Family Links Mitosomal
and Cytosolic Pathways through Exchange of 3'-Phosphoadenosine 5'-Phosphosulfate
and ATP.
Mi-ichi F, Nozawa A, Yoshida H, Tozawa Y, Nozaki T.
Eukaryot Cell. 2015 14:1144-50.

A novel Mitosomal β-barrel Outer Membrane Protein in Entamoeba.
Santos HJ, Imai K, Makiuchi T, Tomii K, Horton P, Nozawa A, Ibrahim M, Tozawa Y,Nozaki T.
Sci Rep. 2015 5:8545.

High-throughput synthesis of stable isotope-labeled transmembrane proteins fortargeted transmembrane proteomics using a wheat germ cell-free protein synthesis
system.
Takemori N, Takemori A, Matsuoka K, Morishita R, Matsushita N, Aoshima M,
Takeda H, Sawasaki T, Endo Y, Higashiyama S.
Mol Biosyst. 2015 11:361-5.

The ligand binding ability of dopamine D1 receptors synthesized using a wheat germcell-free protein synthesis system with liposomes.
Arimitsu E, Ogasawara T, Takeda H, Sawasaki T, Ikeda Y, Hiasa Y, Maeyama K.
Eur J Pharmacol. 2014 745:117-22.

Incorporation of adenine nucleotide transporter, Ant1p, into proteoliposomes facilitates
ATP translocation and activation of encapsulated luciferase.
Nozawa A, Tozawa Y.
J Biosci Bioeng. 2014 118:130-3.

Modifications of wheat germ cell-free system for functional proteomics of plant
membrane proteins.
Nozawa A, Tozawa Y.
Methods Mol Biol. 2014 1072:259-72.

Characterization of the plastidic phosphate translocators in the inducible crassulaceanacid metabolism plant Mesembryanthemum crystallinum.
Kore-eda S, Nozawa A, Okada Y, Takashi K, Azad MA, Ohnishi J, Nishiyama Y,Tozawa Y.
Biosci Biotechnol Biochem. 2013 77:1511-6.

Production and partial purification of membrane proteins using a liposomesupplemented wheat cell-free translation system.
Nozawa A, Ogasawara T, Matsunaga S, Iwasaki T, Sawasaki T, Endo Y.
BMC Biotechnol. 2011 11:35.

Cell-free synthesis, reconstitution, and characterization of a mitochondrial
dicarboxylate-tricarboxylate carrier of Plasmodium falciparum.
Nozawa A, Fujimoto R, Matsuoka H, Tsuboi T, Tozawa Y.
Biochem Biophys Res Commun. 2011 414:612-7.

Robotic large-scale application of wheat cell-free translation to structural studies
including membrane proteins.
Beebe ET, Makino S, Nozawa A, Matsubara Y, Frederick RO, Primm JG, Goren MA,
Fox BG.
N Biotechnol. 2011 28:239-49.

Production of membrane proteins through the wheat-germ cell-free technology.
Nozawa A, Nanamiya H, Tozawa Y.
Methods Mol Biol. 2010 607:213-8.

Cell-free translation of integral membrane proteins into unilamelar liposomes.
Goren MA, Nozawa A, Makino S, Wrobel RL, Fox BG.
Methods Enzymol. 2009 463:647-73.

A cell-free translation and proteoliposome reconstitution system for functional analysisof plant solute transporters.
Nozawa A, Nanamiya H, Miyata T, Linka N, Endo Y, Weber AP, Tozawa Y.
Plant Cell Physiol. 2007 48:1815-20.