For research use only
Not for use in diagnostic procedures

iMatrix-511 silk
Version 004
Store at 2-15°C

Product No. 892 021 1,050 μg
Product description: iMatrix-511 silk is a recombinanthuman laminin-511 E8 fragment protein produced bya silkworm expression system. iMatrix-511 silk
contains the integrin-binding site of the laminin-511
molecule. iMatrix-511 silk is a useful cell culture
substrate for feeder-free culture and single-cellpassage of ES cells and iPS cells, facilitating stableculture expansion. iMatrix-511 silk is also useful forthe culture of other cells adhering to laminin-511.
Content: Recombinant humanfragment protein in PBS(-)

laminin-511

E8

Concentration: 0.5 mg/mL
Amount: 175 μg / 0.35 mL / tube
Product No. 892 021 1,050 μg / 6 tubes
Storage: Store at 2°C to 15°C, protect from light.
Expiration date: The shelf life is 2 years from the dateof manufacture. The expiration date is printed on theouter carton.
Activity: The dissociation constant (Kd) for the binding
with integrin α6β1 is 1 nM or less.
Methods of use: By either of the following methods,
iMatrix-511 silk can be coated onto a culture vessel.
The optimum coating density may differ by cell-type,cell-line, medium selected, or purpose. Insufficientcoating density may result in the detachment of cells
and varied cell conditions while the excessive coating
density may lead to difficulty in detaching cells forpassage.
A. Pre-coating method
Determine the optimal coating density. 0.5 μg/cm2 is astandard but test between 0.1 and 1.5 μg/cm2.
1) Dilute iMatrix-511 silk with PBS(-). Use the diluted
iMatrix-511 silk immediately. To coat with 0.5
μg/cm2 onto a 6-well plate with 9.6 cm2/well, dilute
9.6 μL of iMatrix-511 silk with 2 mL of PBS(-) perwell.
2) Place the diluted iMatrix-511 silk into a culture
vessel and incubate either at 37°C for 1 h, or at room
temperature for 3 h, or at 4°C overnight.
3) Aspirate the coating solution. Then, immediately
seed your cells. Do not allow the coated surface todry.

B. Pre-mixing method
Determine the optimal coating density for cell culture.
The standard density is 0.25 μg/cm2 but test between
0.1 and 1.5 μg/cm2. The optimal coating density may beaffected by the medium and cell density of the cellsuspension.
1) Add iMatrix-511 silk to the cell suspension. To coatwith 0.25 μg/cm2 onto a 6-well plate with 9.6
cm2/well, add 4.8 μL of iMatrix-511 silk to 2 mL ofthe cell suspension per well.
2) Place the cell suspension containing iMatrix-511
silk into a culture vessel.
*If you face difficulties in detaching cells for passage,
re-adjust the conditions (e.g., reduce the coatingdensity).
References:
Taniguchi Y. et al. (2009), J. Biol. Chem. 284 (12):7820-31
Miyazaki T. et al. (2012), Nat. Commun. 3: 1236
Nakagawa M. et al. (2014), Sci. Rep. 4: 3594
Takashima Y. et al. (2014), Cell 158 (6): 1254-69
Miyazaki T. et al. (2017), Sci. Rep. 7: 41165
Kikuchi T. et al. (2017), Nature 548 (7669): 592-6
Goparaju S.K. et al. (2017), Sci. Rep. 7: 42367
Hayashi R. et al. (2017), Nat. Protoc. 12 (4): 683-96
Ishii K. et al. (2018), Stem Cell Reports 10 (2): 568-82
Caution: For research use only. Not intended forhuman use. In the event of accidental ingestion orcontact with the eyes, immediately wash the affectedarea and seek medical attention.
Product information: Current information includingreferences and Q&A are available on the website ofMATRIXOME, Inc. Please use the URL or QR code
below.
Designed by: MATRIXOME, Inc.
3-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Institute for Protein Research, Osaka University
Tel: +81-6-6877-0222 Fax: +81-6-6877-0002
Contact: https://matrixome.co.jp/en/contactURL: https://matrixome.co.jp/en/

Manufactured by: Nippi, Incorporated
1-1-1 Senju Midori-cho, Adachi, Tokyo 120-8601, Japan