For research use only
Not for use in diagnostic procedures

Easy iMatrix-511/Easy iMatrix-511 silk
Product No. 892 018
Product No. 892 024

100 mL (Easy iMatrix-511)
100 mL (Easy iMatrix-511 silk)

Product description: iMatrix-511/iMatrix-511 silk arerecombinant human laminin-511 E8 fragment proteins
expressed in Chinese Hamster Ovary (CHO)-S cells(iMatrix-511) or by a silkworm expression system(iMatrix-511 silk). iMatrix-511/iMatrix-511 silk
contain integrin-binding site of the laminin-511
molecule. iMatrix-511/iMatrix-511 silk are cell culture
substrates useful for feeder-free culture and single-cellpassage of ES cells and iPS cells, facilitating stableculture expansion. Easy iMatrix-511/Easy iMatrix-511
silk are products that have been prepared at a readyto-use concentration of iMatrix-511/iMatrix-511 silk,respectively. Easy iMatrix-511/Easy iMatrix-511 silk
are also useful for the culture of other cells adhering tolaminin-511.
Content: 1.6 µg/mL recombinant human laminin-511
E8 fragment protein dissolved in PBS(-) whichcontains 0.05% recombinant human albumin
Amount: 160 µg / 100 mL
Storage: Store at 2°C to 15°C, protect from light.
Expiration date: The shelf life is 24 months from thedate of manufacture. The expiration date is printed on
the outer carton.

Version 004
Store at 2-15°C2) Incubate either at 37°C for 1 h, room temperaturefor 3 h, or 4°C overnight.
3) Aspirate the coating solution and seed your cells
immediately. Do not allow the coated surface to dry.
*Drying the coated surface may lead inactivation of thelaminin-511 E8 fragment, affecting the cellattachment/detachment efficiency and the cellularcondition.
Caution: For research use only. Not intended forhuman use. In the event of accidental ingestion orcontact with the eyes, immediately wash the affectedarea and seek medical attention.
Product information: Current information includingreferences and Q&A are available on the website ofMATRIXOME, Inc. Please use the URL or QR code
below.
Designed by: MATRIXOME, Inc.
3-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Institute for Protein Research, Osaka University
Tel: +81-6-6877-0222 Fax: +81-6-6877-0002
Contact: https://matrixome.co.jp/en/contact
URL: https://matrixome.co.jp/en/

Activity: The dissociation constant (Kd) for the binding
with integrin α6β1 is 10 nM or less.
Methods of use: By the following procedure, iMatrix511/iMatrix-511 silk can be coated onto a 6-well plate(9.6 cm2/well) with 0.25 µg/cm2. Adjust the usedsolution volume due to the surface area to coat with
0.25 µg/cm2 when other culture vessels are used. The
standard volume is 156 μL/cm2. Insufficient coating
density may result in the detachment of cells andvaried cell conditions while the excessive coatingdensity may lead to difficulty in detaching cells forpassage.
The optimum coating density may differ by celltype, cell-line, medium selected, or purpose. Optimize
the coating volume in culture of cell types other thanES/iPS cells, starting with the standard condition
descripted above.
Procedure: The case of using a 6-well plate (9.6cm2/well)
1) Place 1.5 mL of Easy iMatrix-511/Easy iMatrix-511
silk/well.

Manufactured by: Nippi, Incorporated
1-1-1 Senju Midori-cho, Adachi, Tokyo 120-8601, Japan