【Grant-in-Aid for Scientific Research (S)】Broad Section G
Title ofProject：

Full elucidation of sorting mechanisms in andaround the Golgi apparatus bysuper-resolution live imaging

Akihiko Nakano(RIKEN Center for Advanced Photonics, Deputy Director)
Research Project Number：18H05275 Researcher Number：90142140
Keyword：membrane traffic, Golgi apparatus, sorting and transport
have further improved its performance by raising【Purpose and Background of the Research】
Understanding of the mechanisms of membrane the sensitivity and the speed of detection systemtrafficking is now being totally innovated by (Figure 1). By single-photon counting and a newstate-of-the-art super-resolution live imaging deconvolution algorithm we developed, it now
microscopy. We have recently developed a new enables us to visualize assembly of coat proteins on
method with an extremely high spatiotemporal the organelle membranes, sorting and packaging ofresolution, which can track dynamic 4D behaviors cargo, handover of cargo between compartments,of even vesicles in cytoplasm. With this technology, etc. which we have been eager to unveil.
we will tackle fundamental questions underlying
the transport processes in the secretory pathway, 【Publications Relevant to the Project】
from the ER to the Golgi apparatus and further to ・ Ito, Y., Uemura, T., and Nakano, A. (2018).
Golgi Entry Core Compartment functions as
the trans-Golgi network. Experts on yeast, plant
the COPII-independent scaffold for ER-Golgi
and animal cells will compare correspondingtransport in plant cells. J. Cell Sci.
transport
processes
andextractcommon131:jcs203893.
mechanisms and different features and draw
・Ishii, M., Suda, Y., Kurokawa, K., andcomprehensive models, which will lead to thorough
Nakano, A. (2016). COPI is essential for Golgi
understanding of molecular mechanisms.
cisternal maturation and dynamics. J. Cell
Sci. 129:3251-3261.
【Research Methods】
・Kurokawa, K., Suda, Y. and Nakano, A.
By making full use of SCLIM2 we developed, we(2016). Sar1 localizes at the rims ofwill investigate the following problems.
COPII-coated membranes in vivo. J. Cell
[Yeast cells] 1) cargo capture from the ER bySci. 129:3231-3237.
cis-Golgi; 2) cargo delivery between Golgi cisternae;
・Kurokawa, K., Okamoto, M., and Nakano, A.
3) spatiotemporal regulation of sorting in the TGN.
(2014). Contact of cis-Golgi with ER exit sites[Plant cells] 1) cargo capture from the ER by
executes cargo capture and delivery from theGECCO; 2) cargo delivery in the Golgi stack; 3)
ER. Nat. Commun. 5:3653.
spatiotemporal regulation of sorting in the TGN.
・Uemura, T., Suda, Y., Ueda, T., and Nakano,[Animal cells] 1) cargo capture from the ER by
A. (2014). Dynamic behavior of theERGIC; 2) cargo delivery in the Golgi stack and thetrans-Golgi network in root tissues ofTGN; 3) roles of the Golgi in nerve axons.
Arabidopsis revealed by super-resolution liveimaging. Plant Cell Physiol. 55:694-670.
【Expected Research Achievements and・Suda, Y., Kurokawa, K., Hirata, R., andScientific Significance】
Nakano, A. (2013). Rab GAP cascade
SCLIM, super-resolution confocal live imaging
regulates dynamics of Ypt6 during the Golgi
microscopy, has been a powerful tool to examine
maturation. Proc. Natl. Acad. Sci. U. S.
dynamic processes of membrane trafficking. We
A. 110:18976-18981.
【Term of Project】FY2018-2022【Budget Allocation】148,300 Thousand Yen

Figure 1 SCLIM2
Figures or Pictures【Homepage Address and Other Contact
Information】
https://rap.riken.jp/en/labs/sprg/lcmirt/