Japan-Canada Joint Symposium 2020
“Development and applications of the methods combining
interference/phase measurement techniques and analytic techniques”

日本－カナダ

二国間交流シンポジウム 2020

－干渉・位相計測技術と分析技術の融合を目指して－

May 24 (Sunday) – May 27 (Wednesday), 2020
at
International House, Osaka (planned)

Japanese Society of Microscopy (JSM)
Microscopy Society of Canada (MSC)【はじめに】
日 本 顕 微 鏡 学 会 （ Japanese Society of Microscopy: JSM ） と カ ナ ダ 顕 微 鏡 学 会（Microscopy Society of Canada: MSC）は、両学会に所属する生物・材料系研究者間の相互交流を深め、それにより進歩を続ける電子顕微鏡、並びに科学計測機器のさらなる発展のため、二国間交流シンポジウムを企画しました。初めての試みにあたり、本シンポジウ
ムでは、原子レベルの高分解能法や環境顕微鏡法、そして元素分析技術などの材料系のテーマだけでなく、2017 年のノーベル賞受賞の記憶に新しい生体分子観察法（クライオ電子顕微鏡法）や単粒子解析技術など、生物系テーマを含む広い観点からテーマ・課題を選定し、電子線と物質との相互作用、特に材料科学、デバイス工学、医学、生物学などに共通の課題となっている観察対象へのダメージ、あるいは意図せぬチャージアップなどの影響をも検討することにしました。その結果、日本の得意とする干渉・位相計測技術と、カナダの得意とする分析技術の融合を目指したテーマを選定し、サブテーマ『干渉・位相計測技術と分析技術の融合を目指して』としました。
日本顕微鏡学会第７６回学術講演会と時期を合わせた開催として、より多く、より幅広い分野の研究者の参加と相互交流を目指し、同学術講演会実行委員会とも密接に相談しながら計画を進めました。その結果、1.5 日間のシンポジウム期間中に、21 名の登壇予定者を得ることができ、特にカナダとデンマークから、9 名の参加者となりました。この中には、PosDoc や Dr コースの若手研究者にも加わっていただいており、若手研究者間の交流も期待できるものとしました。
しかしながら、大変残念なことに、本年始めからの新型コロナウイルス感染症の世界的な蔓延により、今回の二国間交流シンポジウムは、講演発表を中止せざるを得ませんでした。そこで、登壇予定者皆さんの予稿原稿や講演プログラムは完成されており準備は整っていましたので、それらを公開することによって、紙上での二国間交流シンポジウムとすることとしました。この冊子は、紙上開催、日本-カナダ交流シンポジウムの予稿集です。
本シンポジウムに参加を予定してくださった研究者の皆さま、本シンポジウム企画に当たりご支援いただきました第 76 回学術講演会実行委員長の高井義造先生と実行委員の皆様方、並びに、本企画にご協力いただいましたすべての皆様方に、誌面を借りて御礼を申し上げます。そして、日本とカナダの両顕微鏡学会の相互交流が今後も継続されることを願っております。
オーガナイザ代表：森 茂生

1【Preface】
The Japanese Society of Microscopy (JSM) and the Microscopy Society of Canada (MSC)
have decided to organize a joint symposium on advanced electron microscopy and its
application in order to deepen mutual understanding and scientific interaction between
researchers belonging to both societies in biological science and materials science and inorder to contribute further development and progress of electron microscopes andscientific measurement instruments. In this first symposium, we selected topics from a
broad perspective, not only from materials science fields including atomic levelhigh-resolution microscopy, environmental microscopy, and elemental analysis, but also
biological science fields including new biomolecule observation method, i.e., cryo
electron microscopy (2017 Nobel Prize), and single particle analyses. During the topic
selection, we also considered the interactions between electron beams and substances,the damage and beam induced charging to the observed samples, and in particularcommon issues affecting materials science, device engineering, medical science, andbiological science. As a result, we came to the conclusion that the symposium should
focus on “Development and applications of the methods combining interference/phasemeasurement techniques and analytic techniques,” particularly discussing electroninterferometry and phase measurement methods that are intensively studied in Japanand analytical and biological microscopies that are intensively studied in Canada.
We decided to hold this symposium jointly with the 76th Annual Meeting of JSM tomaximize participation and deepen interaction and discussion between researchers
from wide scientific fields. Therefore, we prepared the joint symposium in close
collaboration with the JSM Executive Committee of the 2020 Annual Meeting. As aresult, for one-and-a-half day symposium, we got twenty-one speakers, including eightfrom Canada and one from Denmark. Post-doctoral research associated and PhD course
students also planned to join this meeting for make an outstanding networking
opportunity.
We are regret to report that we cannot hold the joint symposium as planned because of
the global spread of new coronavirus infections (COVID-19) starting at the beginning of
this year. Instead, we decided to hold the joint symposium virtually on paper. Since thedraft manuscripts of all speakers and symposium programs were ready, we decided tomake the conference proceeding including them and distribute it to all the planned
participants. This booklet is the proceedings of the Japan – Canada Joint Symposium.

2

We would like to thank all the speakers who scheduled to participate this symposium,
Prof. Y. Takai, Chairman of the 76th Annual Meeting Executive Committee, and all theexecutive committee members, as well as all the people who cooperated with thissymposium project. We hope that mutual relation between JSM and MSC will continue
and expand.
Representative of the organizers,
Shigeo Mori (Osaka Prefecture University)3【ご挨拶】
日本顕微鏡学会長 幾原雄一
本顕微鏡学会では、大阪で開催される予定であった第７６回学術講演会と併設して、日本顕微鏡学会（Japanese Society of Microscopy: JSM）とカナダ顕微鏡学会（Microscopy
Society of Canada: MSC）の二国間交流のシンポジウムの開催を計画していました。このシンポジウムは、現在日本顕微鏡学会が積極的に進めております“国際化へ向けた取り組み”とも合致している上、～干渉・位相計測技術と分析技術の融合を目指して～というタイムリーなテーマが設定されていました。本シンポジウムは、日本学術振興会からの支援も受け、日加二国間のさらなる交流や共同研究の促進が期待されましたが、大変残念なことに、新型コロナウイルス感染症の蔓延により、本シンポジウムにおける講演発表を中止するに至りました。しかし、この記念すべきシンポジウムの企画は是非残したいという関係者の強い要望もあり、この予稿集にみられますように紙上開催は行われました。本シンポジウ
ムをご支援頂きました学術講演会の高井義造実行委員長、本シンポシムの開催にご尽力頂
きました森茂生先生、岡田康志先生、原田研先生ら組織委員の皆様、また、カナダ顕微鏡学会の Marek Malac 会長、Dr. Misa Hayashida をはじめとするカナダ側の組織委員の皆様方に、心から感謝申し上げます。これを機に、日本とカナダの両顕微鏡学会の交流がさらに発展することを祈念しております【Greetings】
Yuichi Ikuhara, President of JSM
The Japanese Society of Microscopy (JSM) planned JSM and the Microscopy Society ofCanada (MSC) joint symposium on advanced electron microscopy and its application
during the period of the 76th JSM Annual Meeting in Osaka. This symposium wasaimed to hold as a part of “Internationalization” that JSM is initiatively promoting, andfocused the very timely topics, “Development and applications of the methods combining
interference/phase measurement techniques and analytic techniques”. This symposium
is supported by JSPS (Japan Society for the Promotion of Science), and has been
expected to enhance the international exchange and mutual collaboration between
two countries. But, unfortunately, we must cancel this symposium due to the global
spread of new coronavirus infections (COVID-19). However, since most of people whoconcern this symposium hope the content of this symposium should be reserved as ourmemorial event, we decided to hold this symposium on paper, as can be seen in thepresent proceedings. I sincerely thank Prof. Yoshizo Takai, the chairman of JSM
meeting, Prof. Shigeo Mori, Prof. Yasushi Okada, Dr. Ken Harada and the symposium
organizers, and also Prof. Marek Malac, the president of MSC, Dr. Misa Hayashida and4

the organizers from Canadian side, as the president of JSM. I really hope that therelationship between JSM and MSC will further continue and enhance ourcollaboration as a starting point for next step.

Marek Malac, President of MSC
It is an honor to have an opportunity to support and participate in the firstJapan-Canada joint symposium. An outstanding scientific program was put together
with a great potential to initiate lasting relations among members of the two societies,
JSM and MSC. It is with great regret that the symposium cannot take place as plannedat Osaka, Japan due to the world wide covid-19 pandemic. I hope that the collaborative
spirit and interest in ongoing connections will lead to a joint workshop or symposium inthe near future. I would like to thank the organizers of the 76th JSM meeting organizers
and to Prof. Takai in particular for their extensive support and understanding. Mythanks also belong to Prof. Ikuhara for his acceptance of the joint symposium and thesupport he kindly provided. Last, but not least, my thanks belong to the symposium
organizers, Dr. Harada, Prof. Mori, Prof. Okada and Dr. Hayashida, for their hard workand dedication that ultimately made the symposium possible.

5【Contents】
1. Time Schedule
2. Program
2.1 Day 1 (May 25 (Monday), 2020)
Opening Address
Materials Science Session 1
Materials Science Session 2
2.2 Day 2 (May 26 (Tuesday), 2020)
Biological Science Session
3. Abstracts
3.1 Day 1 Materials Science Session 1, Materials Science Session 2
3.2 Day 2 Biological Science Session
4. Organizers
5. Participants list
6. Planned Venue Information6【1. Time Schedule】

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【2. Program】
2.1 Day 1 (May 25 (Monday), 2020)
Opening Address

09:00 – 09:05

Yuichi Ikuhara (President of JSM)
Materials Science Session 1Chairperson
Shigeo Mori (Osaka Prefecture University)
Marco Beleggia (Technical University of Denmark)MS1-01 09:05 – 09:35
Understanding Radiation Damage in Beam-Sensitive TEM Samples
Ray Egerton (University of Alberta)
MS1-02 09:35 – 09:55
Capability of a low-kV TEM in the study of beam sensitive materials
Toshie Yaguchi, Yasuhira Nagakubo, Keiji Tamura, Yasuyuki Nodera, Keisuke
Igarashi, Akiko Wakui (Hitachi High-Tech corporation)
MS1-03 09:55 – 10:20
Beam Damage and Sample Preparation in Plasma Focused Ion Beam Microscopy
Peng Dong1, Ali Allahverdi2, Sam Norris1, Hui Yuan1, Nabil D. Bassim1 (1McMasterUniversity, 2Iran University)
Short Break 10:20 – 10:30
MS1-04 10:30 – 11:00
Atomic Resolution Dynamic Observations of Grain Boundary and Surface
Yuichi Ikuhara1,2,3 (1University of Tokyo, 2NSRL, Japan Fine Ceramics Center,3WPI-AIMK, Tohoku University)MS1-05 11:00 – 11:30
Charging of Thin Film Phase Plates in a TEM
Marek Malac1,2, M. Hayashida1, R.F. Egerton2, S. Hettler3, M. Beleggia4 (1NRC-NANO,
2Dept. Physics, University of Alberta, 3INA, University of Zaragoza, 4DTU)

8

MS1-06 11:30 – 12:00
Gas electron holography: realisation and implementationJes Ærøe Hyllested, G. Prabhu Sai Balasubramanian, E. M. Fiordaliso, Marco
Beleggia1 (Technical University of Denmark)Lunch 12:00 – 13:15
Materials Science Session 2
Chairperson
Ken Harada (CEMS, RIKEN)
Marek Malac (NRC-NANO; University of Alberta)
MS2-07 13:15 – 13:45
Towards Atomic Resolution State Analysis by STEM-EELS
Hiroki Kurata, Mitsutaka Haruta (Institute for Chemical Research, Kyoto University)MS2-08 13:45 – 14:10
EDS and EELS of Lithium Materials from 0.5 to 30 keV
Raynald Gauvin, Nicolas Brodusch, Frédéric Voisard (McGill University)MS2-09 14:10 – 14:30
Fusion of Analytical TEM/STEM and in-situ analysis
Hiromi Inada (Hitachi High-Tech Corp.)
MS2-10 14:30 – 14:50
High-precision Phase Analysis of Automatically Collected Electron Holograms
Yoshio Takahashi1, Tetsuya Akashi1, Atsuko Sato2, Yoshiaki Tanigaki1, Hiroyuki
Shinada1, Yasukazu Murakami2 (1Hitachi, Ltd., 2Kyushu University)
Short Break 14:50 – 15:00
MS2-11 15:00 – 15:25
Combination of Fluctuation Electron Microscopy and Ptychography for
Characterization of Amorphous – Crystalline Mixtures
Arthur Blackburn (University of Victoria)

9MS2-12 15:25 – 15:45
Thermal stability and microstructures of the LiNi1/3Mn1/3Co1/3O2 positive electrode for
sulﬁde-based all-solid-state lithium batteries
Hirofumi Tsukasaki, Misae Otoyama, Shigeo Mori, Akitoshi Hayashi, Masahiro
Tatsumisago (Osaka Prefecture University)MS2-13 15:45 – 16:05
Crystallization differences of Al2O3 on GaN planes
Emi Kano1, Kazutaka Mitsuishi1, Yoshihiro Irokawa1, Toshihide Nabatame1, Koji
Kimoto1, Tetsu Kachi2, Yasuo Koide1 (1National Institute for Materials Science,
2Nagoya University)MS2-14 16:05 – 16:30
Higher-order structure of human chromosomes observed by electron tomography andelectron diﬀraction
Misa Hayashida1, Rinyaporn Phengchat2, Marek Malac1, Ken Harada3, Tetsuya
Akashi4, Nobuko Ohmido2, Kiichi Fukui5 (1National Research Council Canada, 2Kobe
University, 3RIKEN, 4Hitachi, Ltd., 5Osaka University)

2.2 Day 2(May 26 (Tuesday), 2020)

Biological Science Session
Chairperson
Yasushi Okada (BRD, RIKEN; University of Tokyo)
Elitza Tocheva（University of British Columbia)
BS-01 08:30 – 09:00
Engineering genetically encoded biosensors of neural activity and metabolism
Robert Campbell1,2 (1University of Alberta, 2University of Tokyo)
BS-02 09:00 – 09:20
Correlative Light and Electron Microscopy (CLEM) for trace of climbing ﬁber
Mitsuo Suga, Hideo Nishioka (JEOL Ltd.)10

BS-03 09:20 – 09:40Single-molecule nanoscopy by using cryogenic ﬂuorescence microscopy
Satoru Fujiyoshi (Tokyo Institute of Technology)
BS-04 09:40 – 10:10
Correlation of cryo super-resolution and cryo-electron tomography in bacteria
Danielle Sexton, Elitza Tocheva (University of British Columbia)
BS-05 10:10 – 10:30
Near-atomic resolution structures of the doublet microtubules by cryo-EM
Muneyoshi Ichikawa1,2, Ahmad Khalifa1, Shintaroh Kubo3, Daniel Dai1, Kaustuv
Basu4, Amin Maghrebi1, Javier Vargas1, Khanh-Huy Bui1 (1Department of Anatomyand Cell Biology, McGill University, 2Department of Systems Biology, Graduate Schoolof Biological Sciences, Nara Institute of Science and Technology, 3Department ofBiophysics, Graduate School of Science, Kyoto University, 4Facility for Electron
Microscopy Research, McGill University)
BS-06 10:30 – 11:00
Development and Applications of a New Cryo TEM, JEOL CRYO ARM
Keiichi Namba (Graduate School of Frontier Biosciences, Osaka University)
BS-07 11:00 – 11:30
Cryo-ED and EM for higher-resolution and higher-precision structure analysis
Koji Yonekura1, Saori Maki Yonekura1, Tasuku Hamaguchi1, Hisashi Naitow1,Kiyofumi Takaba (RIKEN SPring-8 Center)11

【3. Abstracts】
3.1Day 1(May 25 (Monday), 2020)

Materials Science Session 1(Chair: Shigeo Mori and Marco Beleggia)

Materials Science Session 2(Chair: Ken Harada and Marek Malac)

12MS1-01

MS1-0213

MS1-03

MS1-0414

MS1-05

MS1-0615

MS2-07MS2-0816

MS2-09MS2-1017

MS2-11

MS2-1218

MS2-13MS2-1419

【3. Abstracts】
3.2 Day 2(May 26 (Tuesday), 2020)

Biological Science Session(Chair: Yasushi Okada and Elitza Tocheva)20

BS-01

BS-02

21

BS-03

BS-04

22

BS-05

BS-06

23

BS-0724

【4. Organizers】
Shigeo Mori (Osaka Prefecture University)
森茂生 （大阪府立大学）

Yasushi Okada (RIKEN, University of Tokyo)
岡田康志 （理化学研究所、東京大学）
Ken Harada (RIKEN)
原田研 （理化学研究所）

Marek Malac (National Research Council Canada, University of Alberta)
Misa Hayashida (National Research Council Canada)【5. Participants List】
Chairpersons

Alphabetical order

Beleggia, Marco

marco.beleggia[at]cen.dtu.dk

Technical University of Denmark
Harada, Ken

原田研kharada[at]riken.jp

CEMS, RIKEN 理化学研究所
Malac, Marek1,2
1 NRC-NANO

mmalac[at]ualberta.ca

2 University of Alberta

Mori, Shigeo 森 茂生
Osaka Prefecture University 大阪府立大学

mori[at]mtr.osakafu-u.ac.jp

Okada, Yasushi1,2 岡田康志
1 BDR, RIKEN

理化学研究所

2 University of Tokyoy.okada[at]riken.jp

東京大学

Tocheva, Elitza

elitza.tocheva[at]ubc.ca

University of British Columbia

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Speakers

Alphabetical order

Beleggia, Marco

marco.beleggia[at]cen.dtu.dk

Technical University of DenmarkBlackburn, Arthur

ablackbu[at]uvic.ca

University of Victoria
Campbell, Robert1,2
1 University of Alberta

robert.e.campbell[at]ualberta.ca

2 University of Tokyo

Dong, Peng

dongp1[at]mcmaster.ca

McMaster University
Egerton, Rayregerton[at]ualberta.ca

University of Alberta
Fujiyoshi, Satoru 藤芳 暁Tokyo Institute of Technology 東京工業大学
Gauvin, Raynald

raynald.gauvin[at]mcgill.ca

McGill University
Hayashida, Misa

fujiyoshi[at]phys.titech.ac.jp

林田美咲

hayashid[at]ualberta.ca

National Research Council Canada
Ichikawa, Muneyoshi 1,2 市川宗厳
1 McGill University
2 Nara Institute of Science and Technology

michikawa[at]bs.naist.jp

奈良先端科学技術大学
Ikuhara, Yuichi 1,2,3 幾原雄一
1 University of Tokyo

東京大学

2 NSRL, Japan Fine Ceramics Center

ikuhara[at]g.ecc.u-tokyo.ac.jp（財）日本セラミックスセンター
3 WPI-AIMK, Tohoku University

Inada, Hiromi

東北大学

稲田博実

Hitachi High-Tech Corporation

hiromi.inada.yd[at]hitachi-hightech.com

株式会社 日立ハイテク
Kano, Emi 狩野絵美

kano[at]imass.nagoya-u.ac.jp

National Institute for Materials Science(present e-mail address)

物質・材料研究機構

26

Kurata, Hiroki

倉田博基

Institute for Chemical Research, Kyoto

kurata[at]eels.kuicr.kyoto-u.ac.jp

University 京都大学
Malac, Marek1,2
1 NRC-NANO

mmalac[at]ualberta.ca

2 Dept. Physics, University of Alberta

Namba, Keiichi

難波啓一

Graduate School of Frontier Biosciences

keiichi[at]fbs.osaka-u.ac.jp

Osaka University 大阪大学
Suga, Mitsuo

須賀三雄

msuga[at]jeol.co.jp

JEOL Ltd. 日本電子株式会社
Takahashi, Yoshio

高橋由夫

yoshio.takahashi.jc[at]hitachi.com

Hitachi, Ltd. 株式会社 日立製作所
Tocheva, Elitza

elitza.tocheva[at]ubc.ca

University of British Columbia
Tsukasaki, Hirofumi

塚崎裕文

Osaka Prefecture University 大阪府立大学

h-tsukasaki57[at]mtr.osakafu-u.ac.jp

Yaguchi, Toshie 矢口紀恵
Hitachi High-Tech Corporation

toshie.yaguchi.yy[at]hitachi-hightech.com

株式会社 日立ハイテク
Yonekura, Koji

米倉功治

yone[at]spring8.or.jp

RIKEN SPring-8 Center 理化学研究所27

【6. Planned Venue Information】
International House, Osaka (iHOUSE / Osaka Conference Center & Hotel)
2-6 Uehommachi 8-chome, Tennoji-ku, Osaka 543-0001, JapanTel: +81-6-6772-5931 / Fax: +81-6-6772-7600
http://www.ih-osaka.jp/en/index.html

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