Sample Submission Guidelines
Human Whole Genome Sequencing
ServiceSample TypeSample Source

Optimum Input
AmountVolume

Concentration

Purity and QualityhWGS(PCR-free)gDNA

Fresh frozen tissue, cell line,saliva, blood≥ 1.5 µg

≥ 20 µl≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 1.7
DIN ≥ 7gDNA

Fresh frozen tissue, cell line,saliva, blood≥ 500 ng

≥ 20 µl≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 1.7
DIN ≥ 7gDNAFFPE≥ 700 ng

≥ 20 µl≥ 20 ng/µl

Main band of > 1,500 bp(Q Score > 0.5)
DIN ≥ 4hWGS
(PCR-based)

Recommended suspension buffer: TE buffer or Nuclease-free water

Human Whole Exome Sequencing
ServiceSample Type

Sample Source

Optimum Input
AmountVolumeConcentration

Purity and Quality

gDNA

Fresh frozen tissue, cell line,saliva, blood≥ 300 ng

≥ 20 µl≥ 15 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 1.7
DIN ≥ 7gDNAFFPE≥ 500 ng

≥ 20 µl≥ 20 ng/µl

Main band of > 1,500 bp(Q Score > 0.5)
DIN ≥ 4

Purity and QualityhWES

Recommended suspension buffer: TE buffer or Nuclease-free water

RNA Sequencing
ServiceSample Type

Sample Source

Optimum Input
AmountVolumeConcentration

Total RNAFresh frozen tissue, cell line,saliva, blood≥ 400 ng

≥ 20 µl≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 2.0
RIN ≥ 6RNA-Seq
Total RNAFFPE≥ 500 ng

≥ 20 µl≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 2.0
DV200 > 50%
RIN ≥ 2Total RNA

Fresh frozen tissue, cell line,saliva, blood≥ 800 ng

≥ 20 µl≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 2.0
RIN ≥ 6

≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 2.0
DV200 > 50%
RIN ≥ 2RNA-Seq(Directional)
Total RNA

FFPE

≥ 1 µg≥ 20 µl

Recommended suspension buffer: TE buffer or Nuclease-free water

Document No.: MGL-F-504-01

Version No.: 02Effective Date: 01-Jan-2023

Page 1 of 3

Sample Submission Guidelines
Human Whole Genome Methylation Sequencing
ServiceSample TypeSample Source

Optimum Input
AmountVolumeConcentration

Purity and Quality

gDNA

Fresh frozen, cell line, saliva, blood≥ 1.2 µg

≥ 20 µl≥ 20 ng/µl

OD260/280 = 1.8 - 2.0
OD 260/230 ≥ 1.7
DIN ≥ 7gDNA

FFPE≥ 1.2 µg

≥ 20 µl≥ 20 ng/µl

Main band of > 1,500 bp(Q Score > 0.5)
DIN ≥ 4hWGMS

Recommended suspension buffer: TE buffer or Nuclease-free water

DNA Extraction ServiceServiceSample Type

Sample Source

Optimum Input
Amount

gDNA

Blood

3-5ml whole blood

gDNA

Fresh frozen

TBA

gDNA

Cell line

TBA

gDNA

SalivagDNAExtraction

gDNA

TBA
2FFPE

10 mm , 5-10 slides

Pe-made Library Sequencing ServiceService

Library Concentration(measured by Qubit)

Total Amount

Total Volume

Pre-made library sequencing≥ 2 ng/µl≥ 200 ng

≥ 20 µl

Document No.: MGL-F-504-01

Version No.: 02Effective Date: 01-Jan-2023

Page 2 of 3

Sample Submission Guidelines
Shipping Information FAQ
1. How do I prepare and pack my samples for shipping?
Your MiRXES representative will provide you a Service Order Form and please fill in the required
information, and then return it to us by both emailing a soft copy and attaching a printed copy inyour sample package.

a) Ensure all tubes or plates are clearly labelled per your descriptions in the Service Order Formb) Ensure all tubes or plates are tightly sealed to prevent leakage of samplesc) Attach a copy of the printed Service Order Formd) Ensure adequate dry ice is used for the specimen storage & transporte) For Singapore customers: Inform your MiRXES representative to come collect the package
For non-Singapore customers: Ship your sealed package with your preferred courier to theaddress below

For Japanese Customer
Document No.: MGL-F-504-01

Version No.: 02Effective Date: 01-Jan-2023

Page 3 of 3