UriSet 24
24 h Urine Collection Set

Evaluation Studyby:

SARSTEDT AG & Co. KG · P.O. Box 12 20 · D-51582 Nümbrecht · Phone: +49 2293 305 0 · Fax: +49 2293 305 3992
export@sarstedt.com · www.sarstedt.com

The innovative concept of the new UriSet 24 is based on findings determined in a series of numerous tests
and in co-operation with Institute of Clinical Chemistry, University Hospital Großhadern, Munich/Germany.
Apart from other fields of applications, this urine collection set is particularly intended for the collectionof 24 h urines and analysis ofEpinephrine
Metanephrine
Norepinephrine
Normetanephrine
Dopamine			Vanillylmandelic acid
Homovanillic acid
Hydroxyindoleacetic acid

The analysis results outlined below answer the most important questions raised by physicians and laboratories:
1.

Which acid concentration and quantity is required for optimum long term stabilization of the abovementioned marker molecules in 24 h urines?

2.

Which capacity must a urine container have to hold most 24 h urines?

3.

Which are the most suitable storage conditions for the urine sample between collection and analysis?

1. Acid ConcentrationPooled urines were spiked and aliquots adjusted to show different pH values (in 13 steps of 0.5 pH units frompH = 0.5 to pH = 6.5). The test was established to determine if the samples remained stable when stored in adark place at room temperature.
Values ranging within the limits of methodical measuring accuracy (illustrated by grey sections in the diagrams)
were considered an optimal pH range.
To simplify the graphics, the following diagrams on the chronological development (7 days) of percentage
recovery show only four of the 13 pH steps measured, i.e. pH values 0.5 (orange), 2.5 (green), 5.0 (blue) and6.5 (violet).

Recovery of analytes (shown in per cent) over 7 days
Urines of pH steps: ---0.5 ---2.5 ---5.0 and --- 6.5

Recovery in %Recovery in %days

Recovery in %

days

days

Recovery of analytes (shown in per cent) over 7 days
Urines of pH steps: ---0.5 ---2.5 ---5.0 and ---6.5
Recovery in %Recovery in %days
Recovery in %Recovery in %days
Conclusion:		

Recovery in %

days

days

All diagnostic parameters shown are stable in a pH range between 2.5 and 5.0 for a periodof 7 days, stored in a dark place at room temperature.

To determine the suitable acid concentration and amount, theurine volume and buffer quality from a representative group ofindividuals was analysed by titrating seventy-eight 24 h urines.
The amount of acid required to obtain the pH range of 2.5 and
5.0 for the majority of these urines is based on these data.
The histogram on the right shows that adding 9 ml 20 % HCl
shifts the pH of 62 out of the 78 urines into the target range(green bars = 79.5 %).
For the catecholamines and metanephrines, the 7 urines
indicated in blue are suitable as well (88.5 %).
Conclusion:		days

For the vast majority of urines, 9 ml of 20 % HCl
shift the urinary pH into the required range.

2. Urine Container Capacity
116 Adult test subjects were requested to collect 24 h urines without changing their drinking habits.

Ask the patient about his/her drinking habits and how often he/she uses the toilet,
and hand out two Urine Collection Sets, as required.

3. Sample Storage
The stability of the analytes stated above is mainly determined by pH,temperature and light exposure.
Samples stored in daylight and at room temperature showed a distinctive
decline in the analytes’ recovery values as shown by the graph on theright, illustrating the extremely light-sensitive epinephrine and the preset
pH values of 4.5 and 5.0.
The broken lines indicate the course of epinephrine values when urine isstored in transparent tubes while the continuous lines are indicative ofstorage in the dark as in SARSTEDT transport tubes.
The percentage transmission shows the amount of light that can passthrough the transport tube and the urine container (i.e. through its brown
coloured body and the transparent inspection strip).

BRO_20_375_0000_200_NF

In particular, the short-wave, high-energy ultraviolet light (withwavelengths below 400 nm) is detrimental to the analytes.

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Recommendation:			Technical modifications reserved

For 91.4 % of the test persons (106), a 3 L container was found to hold the urines collected;
10 test persons exceeded 3 L.

The materials and colouration of the urine container and transport tube were selected according to the best
light protection.