Product datasheet
anti-p53 Protein mouse monoclonal, Bp53.11, liquid, purified
Short overview
Cat. No.

690039Quantity

1 ml (50 µg/ml)

Concentration

50 µg/ml (50 µg)

Product description
HostMouse

Antibody Type

Monoclonal

Isotype

IgG2aClone

Bp53.11

Immunogen

Recombinant human p53 (transcription domain within the NH2 terminus)

Formulation

PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA

UniprotID

Q96A56 (Human)

SynomymTumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent
damage-inducible nuclear protein 1, p53DINP1, TP53INP1, P53DINP1, SIP

Conjugate

UnconjugatedPurification

Affinity chromatography

Storage

Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles

Intended use

Research use only

Application

ICC/IF, IHC, WB

Reactivity

Human

Applications
Immunocytochemistry (ICC)

Assay dependent

Immunohistochemistry (IHC) - frozen

1:1,000-1:3,000 (17-50 ng/ml)

Immunohistochemistry (IHC) - paraffin 1:1,000-1:3,000 (17-50 ng/ml, microwave treatment recommended)
Western Blot (WB)

1:2,000-1:10,000 (5-25 ng/ml)

BackgroundExcellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31. The antibody
stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, headand neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.Reactivity on cultured cell lines: Hela, MCF-7.

Product images

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Tel.: +49 (0) 6221 8278-0 | Fax: +49 (0) 6221 8278-24 | Email: info@progen.com | Web: www.progen.com
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p53 staining in human squamous cell carcinoma (image courtesy of J.Heß, University Hospital Heidelberg)

p53 staining in human squamous cell carcinoma (image courtesy of J.Heß, University Hospital Heidelberg)

Western blot analysis of HEK lysate with anti-p53 antibody. Western blot analysis was performed on 20 µg HEK lysate. The PVDF membrane wasblocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-p53, mouse monoclonal, Bp53.11 (Cat. No. 690039)
was diluted in blocking buffer (antibody concentration 25 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goatpolyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands werevisualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.

PROGEN Biotechnik GmbH | Maaßstraße 30 | D-69123 Heidelberg
Tel.: +49 (0) 6221 8278-0 | Fax: +49 (0) 6221 8278-24 | Email: info@progen.com | Web: www.progen.com
2024 September 28 / Version: 690039/DS-220222lim | Page 2

References
Publication

Species

Application

Deng, Z. et al. Methylation of CpG sites in the upstream

human

IHC

regulatory region, physical status and mRNA expression ofHPV-6 in adult-onset laryngeal papilloma. Oncotarget. 8,85368-85377 (2017)
OhtakÃ©, S. et al. Precancerous foci in pleomorphic adenoma human

IHC (paraffin)of the salivary gland: recognition of focal carcinoma andatypical tumor cells by P53 immunohistochemistry. J. OralPathol. Med. 31, 590â€“7 (2002).

Homann, N. et al. Overexpression of p53 in tumor-distanthuman

IHC (frozen)

epithelia of head and neck cancer patients is associated withan increased incidence of second primary carcinoma. Clin.
Cancer Res. 7, 290â€“296 (2001).
Fack, F. et al. Epitope mapping by phage display: Random

epitope mapping

versus gene-fragment libraries. J. Immunol. Methods 206,43â€“52 (1997).

Vispo, N. S., AraÃ±a, M. J., Chinea, G., Ojalvo,Â a G. &epitope mapping

Cesareni, G. Characterisation of epitopes on human p53 using
phage-displayed peptide libraries: insights into
antibody-peptide interactions. Hybridoma 18, 251â€“255(1995).

PROGEN Biotechnik GmbH | Maaßstraße 30 | D-69123 Heidelberg
Tel.: +49 (0) 6221 8278-0 | Fax: +49 (0) 6221 8278-24 | Email: info@progen.com | Web: www.progen.com
2024 September 28 / Version: 690039/DS-220222lim | Page 3