September 2nd, 2022
FOR IMMEDIATE RELEASE
Company name
Representative
Inquiries
Phone

Premier Anti-Aging Co., Ltd.
TSE : 4934
Kiyoshi Matsuura, President
Yuka Uehara,
Head of Corporate Communication Division
and Executive Officer+81-3-3502-2020

Notice concerning a development and commercialization ofnovel dental pulp-derived stem cell culture supernatant by the research collaboration
between University of Tokyo and the consolidated subsidiary
Premier Wellness Science Co., Ltd. (Headquarter: Minato-ku, Tokyo, Chief Executive Officer: Shinji
Hosoyama) , a consolidated subsidiary of Premier Anti-Aging Co., Ltd., and the University of Tokyo(President: Teruo Fujii) are pleased to announce that they have successfully developed and commercialized
a new cosmetic ingredient derived from mesenchymal stem cell culture supernatant called “ENGY Stem S".
The ingredient is derived from a unique dental-pulp stem cell culture supernatant, specialized for skin care,as the first result of the joint research project "Development and Commercialization of Novel Functional
Materials" started on March 22, 2021.
The financial impact of this new technological development is negligible.

News Release

September 2nd, 2022

Premier Wellness Sciences Co., Ltd.

The University of Tokyo and Premier Wellness Sciences Co., Ltd. havesuccessfully developed and commercialized ENGY Stem S, a unique dental
pulp-derived stem cell culture supernatant, specialized in skin care.
~Demonstrated usefulness in human studies and identified new possibilities for skin care~
Premier Wellness Science Co., Ltd. (Headquarter: Minato-ku, Tokyo, Chief Executive Officer:Shinji Hosoyama, hereinafter “PWS”) and the University of Tokyo (President: Teruo Fujii) arepleased to announce that they have successfully developed and commercialized a new cosmetic

ingredient derived from mesenchymal stem cell 1 culture supernatant 2. The new ingredient, ENGY

Stem S , is a unique dental pulp stem cell culture supernatant specialized for skin care. This is the first
result of the joint research project that began on March 22, 2021.

Features of “ENGY StemS”; the original dental pulp stem cell culture supernatant-Stem cell culture supernatant derived from human adult dental pulp 3 that can produce morevaluable factors comparing with other stem cells.

- High quality original cell culture method established by the University of Tokyo that can produce
more valuable factors with high skin improvement effect.

-Traceability system that ensures transparency through collaboration with the University of TokyoHospital.

-High quality assurance (standardization of safety tests, INCI registration of PCPC cosmetic raw
materials in the U.S., SDS safety data sheet regulations, etc.)-Evaluated efficacy in humans and elucidated the mechanism (key factors in the culture medium).

1. Background of Joint Research and Development:
Stem cell therapy has been booming in the field of regenerative medicine. Stem cells have been thefocus of attention in cell therapy, however, recently the usefulness of "stem cell culture supernatant," aculture medium containing biological active factors secreted from cells has been discovered and isexpected to be as effective as transplantation of stem cells. Based on this background, it is expected tohave applications in not only for medicine but also in anti-aging and others. In contrast, there areexisting challenges that need further research before practical application, such as highly transparent
traceability, several safety assessments, scientific evaluation of the active ingredients in the culture
1Cells that are the main source of body cells (somatic cells). There are different types of stem cells for different parts of the body andorgans, such as fat, umbilical cord, and skin. Stem cells that are known to have the function of repairing and replenishing cells damaged
by injury, disease, aging, etc.

2Medium (supernatant) containing useful factors secreted from stem cells.

3Nerve of the toothmedium, and demonstration of efficacy in humans. To work through these challenges, we conductedjoint research with the University of Tokyo, and as the first result of this research, we have succeeded indeveloping and commercializing ENGY Stem S, a unique dental pulp stem cell culture supernatantsolution specialized for skin care.

2. What is ENGY Stem S?
ENGY Stem S is a stem cell culture supernatant derived from adult human dental pulp. Based onresearch, the dental pulp was selected because it contains more growth factors than other mesenchymalderived stem cell culture supernatants like fat and bone marrow. Furthermore, we help develop
University of Tokyo's original cell culture method as well as our own stem cell culture supernatant"ENGY Stem S," which is full of nutrient-rich substances (proteins, exosomes 4 ) effective for skin care
(Figure 1). Throughout the research, we committed to strict quality-control measures reviewing donor
management and traceability in collaboration with the University of Tokyo Hospital. Furthermore, wehave succeeded in identifying factors useful for skin care by identifying all proteins in stem cell culture
supernatant, which had rarely been conducted previously.
OriginHuman adult dental pulp stem cells

Culture method

The University of Tokyo's original culture medium and culture method (cannot be disclosed
due to IP rights).

Contained protein
speciesMore than 2,145 proteins (global analysis by DIA proteome analysis 5 ).

Traceability

Donor with consent obtained at the University of Tokyo Hospital.

SafetyMeet safety criteria for biological products, such as microbiological and viral tests.

Transparent traceability through ID management.

Meet safety criteria for topical application such as primary skin irritation and skinsensitization.

Figure 1: Advantages of ENGY Stem S:
Comparison with adipose-derived stem cell culture mediumn=5, mean±SD, student t-test, *<0.05

3. Development of skin care products based on the results of research results:
4Exosomes are Lipid-encapsulated capsules that are released from cells for communication with each other cells. Exosomes containmRNA and protein and have the function of transmitting information between cells. In recent years, exosomes have become known as afactor controlling the regenerative function of stem cell culture supernatant.
5: Cutting-edge analysis technology that can analyze and identify global proteins contained in a solution to determine what kind ofproteins are contained and in what quantities. It is widely used in medical research such as cancer and Covid-19 research.

ENGY Stem S is planned to be commercialized in Premier Wellness Science's new brand to be launchedthis fall.
4. Overview of Both Parties:■ Premier Wellness Science Co., Ltd.
FoundedChief Executive
Officer
Address
Specialization

December 2020
Shinji Hosoyama
Tranomon Hills Mori Tower, 1-23-1 Toranomon, Minato-ku, Tokyo, Japan
R&D and product development of product categories, such as health, beauty, anti-aging,orts, and business consulting services

URL

https://www.p-wellnessscience.co.jp/OwnershipPremier Anti-Aging Co., Ltd. (TSE Growth: 4934)■ The University of TokyoPresident

Teruo Fujii

Address

7-3-1 Hongo, Bunkyo-ku, Tokyo
Professor Kazuto Hoshi, Department of Oral and Maxillofacial Surgery and Orthodontics,PI

The University of Tokyo Hospital

URL

https://www.u-tokyo.ac.jp/

5. For interviews and inquiries regarding this release:
Premier Wellness Sciences, Co., Ltd.

PR/IR contact: pws-prir@p-wellnessscience.co.jp
As part of our measures to prevent the infection of the new coronavirus, our group is working by telework. Pleasecontact us by using the above e-mail address for inquiries.

6. Regarding This Release:
The information in this news release is current as of the date of publication. It is subject to change
without notice due to various factors. Please understand this in advance.