HIRANUMA APPLICATION DATA

Data No. B15

Automatic Titrator

Sep. 12,2018

Sodium chloride inperitoneal dialysis solution

Drugs andMedicines
1. Abstract

This report introduces an example of measurement for sodium chloride in peritoneal dialysis solution with
precipitation titration. The sample is acidified by nitric acid and the concentration of sodium chloride isdetermined by precipitation titration with silver nitrate titrant using the silver / reference electrode.
NaCl＋

AgNO3

→

AgCl＋NaNO3

2. Configuration of instruments and Reagents(1) Instruments
Main unit ：Hiranuma Automatic Titrator

COM Series

Electrode ：Silver combination electrode

AGR-811Z (Double Junction Type)*The following electrodes are also usable.
・AGR-801Z (Silver reference combination electrode)・Combination of AG-311 (Silver indicator electrode) and MS-231Z (Silver referenceelectrode)・ Combination of AG-311 and RE-241Z (Double junction type silver referenceelectrode)* Remark
The general reference electrode (RE-201Z) cannot be used for this titration because
KCl inner solution might come out to sample solution and it causes measurement
error.
The inner electrodes of AGR-801Z and MS-231Z use mercury (I) sulfate. Whenthese electrodes are disposed, please ask local industrial waste disposal operator.
(2) Reagents
Titrant：

Additive ：0.1 mol/L Silver nitrate standard solution
1 mol/L Nitric acid

3. Measurement procedure(1) Take 10 ml of sample into 100 mL beaker with volumetric pipette.
(2) Add 40 ml of DI water.
(3) Add 2 ml of 1 mol / L nitric acid.
(4) Immerse the electrodes and titrate with 0.1 mol/L silver nitrate standard solution.

4. Measurement conditions and results
Examples of titration conditions
Measurement of blank
Cndt No.

1

MethodAutoConstantNo.

11Size0

Amp No.

2BlankD. UnitmV

Molarity

S-Timer

10sec

Factor

C.P. mL

0mL

T Timer

0sec

D.P. mL

0mL

End Sens

500

Over mL

0.1

Max.Vol.

1Buret No.

Mode No.

14mL

Pre Int

00mL

Del K

00.1

mol/L

Del Sens

0mV

1.001

Int Time

5sec

K0

Int Sens

3mV

L

0Brt Speed

2Pulse8UnitmL

mL

FormulaDmLDigits

3Auto In Pram.

sec

0.01

mLNon

Measurement of sample
Cndt No.

2Method

AutoConstantNo.

2Mode No.

4Buret No.

1Size

10mL

Pre Int

0Amp No.

2Blank

0.015

mL

Del K

9D. UnitmVMolarity

0.1

mol/L

Del Sens

0mV

S-Timer

10sec

Factor

1.001

Int Time

3sec

C.P. mL

0mLK

58.44

Int Sens

3mVT Timer

0sec

L

0

Brt Speed

2D.P. mL

0mL

Pulse

40

End Sens

500

Over mL

0.1

mL

FormulaMax.Vol.

20mL

Digits

Unit

Auto In Pram.

g/L

0.05

(D-B)*K*F*M/S
4NonsecmLMeasurement results
Measurement of sample

Measurement of blank
Measurement

Size

Titrant

Measurement

Size

Titrant

Sodium chloride

No.

(mL)

volume (mL)

No.

(mL)

volume (mL)

(g/L)

1-

0.017

1

10.497

6.1318

2

-

0.013

2

10.4906.127710.5096.1388

Avg. (g/L)

6.133

SD (g/L)

0.006

RSD (%)

0.092

3-

0.015

3

StatisticalresultAvg. (mL)

0.015

StatisticalresultMeasurement of blank

10Measurement of sample

Examples of titration curves

5. NoteIf the precipitate adheres to the electrode after measurement, please clean it and remove it.
Chloride in peritoneal dialysate solution may contain not only sodium chloride but also calcium chloride
and magnesium chloride. Please note that the chloride to be measured is calculated by converting these
combined amounts into sodium chloride.
Keywords：Peritoneal dialysate solution, Sodium chloride, Precipitation titration, Silver electrode, Silvernitrate*Some measurement would not be possible depending on optional configuration of system.