Fast and Simple
Sample Preparationfor Biomolecules
AdvanceBio Spin columns

Improve your data and protect your instrumentation with analytical- and semipreparative-scale
spin columns and 96-sample plates for desalting or buffer exchange of biomolecules.
Elevate your protein and oligonucleotide workflows with a quick and easy sample preparation step to both
improve the quality of your data and protect your LC columns and MS instrumentation. Agilent AdvanceBio Spincolumns are designed for use with a centrifuge, and samples are ready for analysis in just 10 to 15 minutes.

Experience application flexibility with three different formats
AdvanceBio Spin columns are gel-filtration-based products available in three different formats for application
flexibility: analytical- and semipreparative-scale columns for single samples, and 96-sample plates for highthroughput settings. The column bed contains the same cross-linked dextran particles in all three formats, sousers can easily scale up or down in sample number or volume. Choose from:– Analytical-scale spin columns: for individual samples of up to 100 µL; perfect for LC/MS samples– Larger, semipreparative-scale spin columns: for individual samples of up to 1 mL; useful for protein oroligonucleotide samples– Analytical-scale 96-sample plates: for multiple samples of up to 50 µL; used for high-throughput settings
Not
Not
Not
Not
20 µL 30 µL 40 µL 50 µL tested tested 20 µL 30 µL 40 µL 50 µL tested tested

1234

A 99.6%
B 99.9%6799.9%89101112100%

99.9% 99.9%

C99.7%

D 99.9%

99.9%

99.8%

E99.9%

F99.9%

99.7%

GH5NaCI
removal (%):

99.6%99.9%

99.5%

Figure 1. Agilent AdvanceBio
Spin columns and 96-sample
plates are highly effective atremoving salt. Salt removal isconsistently high across the96-sample plate at a variety ofsample volumes.

Better prepare your sample matrix and buffer
When monitoring critical quality attributes, the sample matrix and buffer should be optimal for your analysis. With AdvanceBio Spincolumns, you can quickly and effectively remove excess salts or small molecules from your sample matrix, or exchange the sample
matrix from one buffer to another, offering the following benefits:– Protected instrumentation: For MS analysis, too muchsalt or a nonvolatile buffer can both reduce sensitivity,
leading to poor mass accuracy, and make the instrument
dirty, forcing downtime to clean it. Use AdvanceBio Spincolumns to lower the salt concentration (Figure 1) or
exchange a nonvolatile buffer for a volatile, MS-friendly
buffer (Figure 2).
– Better peak shape and reduced clogs: When your protein
or oligo matrix is incompatible with chromatography, thiscan lead to precipitation, which causes clogs and poor peak
shape, leading to poor resolution. AdvanceBio Spin columns
keep your sample matrix compatible with your starting
conditions to get better peak shape and reduced clogs.

– Eliminated reaction by-products and excess reagentsfrom oligo synthesis: Certain assays for smaller oligos
like microarrays, sequencing, or qPCR primers only requirea quick cleanup. With AdvanceBio Spin columns, you
can quickly remove small-molecule by-products from thesynthesis, cleavage, and deprotection steps.
– Improved sensitivity through reduced matrix
interferences: Buffers can interfere with labelingreactions. For example, primary amine buffers likehistidine can become labeled by Agilent InstantPC,
instead of your released N-glycans (Figure 3). Use
AdvanceBio Spin columns for buffer exchange to ensure
you’re getting the best possible sensitivity.

Before desalting

After desalting

Figure 2. Reversed-phase LC/MS analysis
of rituximab formulated in histidine buffer,containing sodium citrate and polysorbate 80
with and without desalting using an Agilent
AdvanceBio Spin column. The insert mass
spectra show how much contaminatingdetergents are removed from the sample,
prolonging the life of the LC column andmaximizing uptime of the instrument bykeeping it cleaner.

Without buffer exchangeWith buffer exchange

Figure 3. Agilent InstantPC-labeled histidine, detected inrituximab samples without buffer exchange (top) and withbuffer exchange (bottom). The histidine buffer is much
more abundant than the released glycans in the sample andcompetes with the glycans to react with the InstantPC label.

Ordering information
Description

Part Number

AdvanceBio Spin columns for desalting or buffer exchange,< 100 µL samples, 25/pk, collection tubes included

1980-1103

AdvanceBio Spin 96-sample plate for desalting or buffer
exchange, 10–50 µL samples, 1/pk

1980-1104

AdvanceBio Spin columns for desalting or buffer exchange,< 1000 µL samples, 50/pk columns + four reusable adapters

1980-1105

AdvanceBio Spin column reusable adapters, 8/pk
For optional use with p/n 1980-1105

96-well plate, 1.2 mL, round wells, U shape,
polypropylene, 27 mm, 25/pk

1980-1106

5043-9308

Recommended for wash steps with p/n 1980-1104

96-well plate, 0.33 mL, round wells, V shape, polypropylene,
14 mm, 25/pk

5043-9312

Recommended for final collection step with p/n 1980-1104

Sealing mat, 96 wells, silicone, 50/pk

5042-1389

Centrifuge tube, polypropylene, graduated, 29 mm od, 115 mm,conical base, wide neck, threaded top, 50 mL, 25/pk

5610-2049

Centrifuge tube, polypropylene, graduated, 29 mm od, 115 mm,skirted conical base, wide neck, threaded top, 50 mL, 500/pk

190065200

www.agilent.com/chem/advancebio
DE27744541
This information is subject to change without notice.
© Agilent Technologies, Inc. 2024
Published in the USA, April 2, 2024
5994-7305EN