Department of Nano-Bioscience, Graduate School of
Medical and Dental sciences, Tokyo Medical and Dental
University
Location: Tokyo Medical and Dental University Building 22, 2-3-10 Kanda
Surugadai, Chiyoda-ku, Tokyo 101-0062
MAIL aso.orth@tmd.ac.jp

Professor

Yoshinori ASOU M.D.Ph.D.

Professor Kunikazu TSUJI Ph.D
Visiting professor Dai YAMANOUCHI M.D.Ph.D.
Assistant Profecssor

Hailati AINI Ph.D

OVERVIEW
With national healthcare costs now exceeding 40 trillion yen, preventive medicine is becoming
increasingly important with the goal of extending healthy life expectancy. Aging is a risk factor formusculoskeletal diseases such as osteoporosis/sarcopenia/osteoarthritis, lifestyle-related diseases,
cardiovascular disorders, and cancer. In recent years, it has become clear that aging changes in the bodyare not merely physical deterioration, but physiological activities that are actively regulated bymolecular signals. This fact means that it is possible to prevent aging changes by intentionallymanipulating molecules. With the goal of elucidating the mechanisms of aging, disease onset, andmetabolic disorders, we have been studying aging regulators such as sirtuin genes and PAI-1(Plasminogen Activator Inhibitor-1),gut microbiota, HIF (Hypoxia Inducible Factor Factor), etc., and have

been conducting functional evaluation and product development. We will continue to conduct researchunder two banners: regenerative medicine for age-related disorders and preventive medicine to delay
aging itself.

RESEARCH CONTENTS(1) Basic/clinical research on regenerative medicine using nanofibers as a carrier and cell therapy using
mesenchymal stem cells
Technology to regenerate organs lost due to aging, trauma, or disease has been advancing rapidly inrecent years. We are focusing on nanofibers as a next-generation regenerative medicine. Nanofibers arematerials composed of extremely thin fibers less than 1 micron in diameter. We are developing
nanofiber materials optimized as carriers of cells and growth factors to promote regenerative medicine.
In Japan, ahead of the rest of the world, legislation has been established to ensure the safety of cellbased regenerative medicine, and cell therapy using mesenchymal stem cells is becoming increasingly
popular. However, there are still many issues to be addressed, including the lack of evidence for celltherapy and tissue regeneration of sizes and sites that are difficult to overcome using current
technology. We will continue our basic and clinical research to overcome these issues.
(2) Elucidation of the mechanisms of aging, disease onset, and metabolic disorders
In recent years, nicotinamide mononucleotide (NMN) has been attracting attention as a health foodbecause it is expected to activate the longevity gene sirtuin and rejuvenate the body. For many years,
we have focused on SIRT6, one of the sirtuin genes, and studied the aging mechanism of bone andcartilage tissue, and found that a decrease in SIRT6 activity with aging is a cause of osteoporosis andosteoarthritis. We will examine the effects of NMN on the aging process in the body, especially in bone
and cartilage, and will examine the effect of PAI-1 (Plasminogen Activator Inhibitor-1), which is a marker
of cellular senescence and induces cellular senescence on its own, on the aging process in the body. We
found that SIRT6 and PAI-1 cross-talk and cause age-related changes, and that PAI-1 inhibition prevents
postmenopausal osteoporosis and age-related muscle weakness.

(3) Specific Clinical Trial: Efficacy of PAI-1 Inhibition TM-5614 in the Treatment of FGF23-AssociatedHypophosphatemic Rickets and Osteomalacia
FGF23 is a hormone produced mainly in bone cells and plays a central role in the physiological regulationof blood phosphorus levels. FGF23-associated hypophosphatemic rickets/osteomalacia is a rare diseasecaused by excessive production of FGF23. Recently, it has been shown that PAI-1 inhibition promotes
the degradation of FGF23. We will promote a specific clinical study "Exploratory clinical study toinvestigate the efficacy and safety of TM5614 for congenital FGF23-related hypophosphatemic rickets
and osteomalacia" using an orally available small molecule PAI-1 inhibitor TM-5614 to develop a novel
therapeutic agent.

(4) Verification of the effect of oxygen nanobubble administration on hypoxemia

Through joint research with Gifu University, we have demonstrated that drinking oxygen nanobubble
water prevents hypoxemia caused by respiratory depression under general anesthesia using beagle
dogs. These results suggest that oxygen nanocapsule water may improve the ability to work underhypoxic conditions.

(5) Verification of antiviral effect of silver ionized water/effect on infectious diseases
We will verify the antiviral effect, its persistence, and concentration dependence of silver ionized water,which has been widely known for its antimicrobial effect in the past, and develop it for the purpose ofcountermeasures against COVID-19.

(6) Elucidation of aging control mechanism by intestinal bacteria
An enormous number of intestinal bacteria (several hundred trillion) inhabit the intestines. In recent
years, attention has been paid to the functions of gut microbiota, and it has become clear that a varietyof networks are organized between gut microbiota and organs. Our research focuses on the regulatory
mechanism of skeletal tissues by intestinal bacteria. Proanthocyanidins, one of polyphenols, have been
attracting attention for their antioxidant effects, but their large molecular weight means that they are
rarely absorbed through the digestive tract. In collaboration with Tohoku University, we have found thatoral intake of anthocyanidins changes the gut microbiota and prevents bone loss and obesity in a mouseovariectomized model. The gut microflora, which changes with aging, is a cause of various diseases. We
are analyzing the mechanism and approaching the prevention of aging of the locomotor organs from thegut.

Accepted papers
1. Effects of long-term plate fixation with different fixation modes on the radial cortical bone indogs. Muroi N, Ochi H, Shimada M, Asou Y, Hara Y. PLoS One. 2021 Feb
19;16(2):e0247410.
2. A novel PAI-1 inhibitor prevents ageing-related muscle fiber atrophy. Aihemaiti A, Yamamoto
N, Piao J, Oyaizu T, Ochi H, Sato S, Okawa A, Miyata T, Tsuji K, Ezura Y, Asou Y. Biochem
Biophys Res Commun. 2021 Jan 1;534:849-856.
3. Proanthocyanidin-rich grape seed extract improves bone loss, bone healing, and implantosseointegration in ovariectomized animals. Tenkumo T, Aobulikasimu A, Asou Y, Shirato M,
Shishido S, Kanno T, Niwano Y, Sasaki K, Nakamura K. Scientific Reports 2020 Jun
1;10(1):8812. IF 4.120
4. Spinal Cord Ischemia Rescue after Hybrid Total Arch Repair with Frozen Elephant Trunk: A
Case Report. Leung AD, Bartline PB, Acher CW, Yamanouchi D. Ann Vasc Surg. 2020 Feb
4. pii: S0890-5096(20)30127-8. IF 1.179
5. Osteoclast-Like Cells in Aneurysmal Disease Exhibit an Enhanced Proteolytic Phenotype.
Kelly MJ, Igari K, Yamanouchi D. Int J Mol Sci. 2019 Sep 21;20(19). IF 4.183
6. Digoxin Attenuates Receptor Activation of NF-κB Ligand-Induced Osteoclastogenesis in
Macrophages. Igari K, Kelly MJ, Yamanouchi D. J Vasc Res. 2019;56(2):55-64. IF 1.855
7. Cigarette Smoke Extract Activates Tartrate-Resistant Acid Phosphatase-Positive

Macrophage. Igari K, Kelly MJ, Yamanouchi D. J Vasc Res. 2019;56(3):139-151. IF 1.855
8. Expression of type II collagen and aggrecan genes is regulated through distinct epigenetic
modifications of their multiple enhancer elements. Nham GTH, Zhang X, Asou Y, Shinomura
T. Gene. 2019 Jul 1;704:134-141. IF 2.638
9. RANKL-mediated osteoclastogenic differentiation of macrophages in the abdominal aorta ofangiotensin II-infused apolipoprotein E knockout mice. Tanaka T, Kelly M, Takei Y,
Yamanouchi D. J Vasc Surg. 2018 Dec;68(6S):48S-59S.e1. IF 3.243
10. Development of single nanometer-sized ultrafine oxygen bubbles to overcome the hypoxiainduced resistance to radiation therapy via the suppression of hypoxia-inducible factor‑1α.
Iijima M, Gombodorj N, Tachibana Y, Tachibana K, Yokobori T, Honma K, Nakano T, Asao
T, Kuwahara R, Aoyama K, Yasuda H, Kelly M, Kuwano H, Yamanouchi D. Int J Oncol.
2018 Mar;52(3):679-686. IF 3.571
11. Case report of retrograde in situ fenestration of the thoracic stent graft with reentry device ina patient with aortobronchial fistula. Leung AD, Yamanouchi D. Medicine (Baltimore). 2018
Jun;97(24):e11050. IF 1.870
12. Osteoclastogenesis in Abdominal Aortic Aneurysms: A New Therapeutic Target. Leung AD,
Yamanouchi D. Curr Drug Targets. 2018;19(11):1233-1240. IF 2.642
13. A small-molecule PAI-1 inhibitor prevents bone loss by stimulating bone formation in a murine
estrogen deficiency-induced osteoporosis model. Jin G, Aobulikasimu A, Piao J, Aibibula Z,
Koga D, Sato S, Ochi H, Tsuji K, Nakabayashi T, Miyata T, Okawa A, Asou Y. FEBS Open
Bio. 2018 Feb 16;8(4):523-532. doi: 10.1002/2211-5463.12390. IF 1.782
14. Proanthocyanidin-Rich Grape Seed Extract Modulates Intestinal Microbiota in Ovariectomized
Mice. Jin G, Asou Y, Ishiyama K, Okawa A, Kanno T, Niwano Y. J Food Sci. 2018
Apr;83(4):1149-1152. doi: 10.1111/1750-3841.14098. Epub 2018 Mar 26. IF 2.018
15. Proanthocyanidin-rich grape seed extract prevent estrogen deficiency-induced metabolic
disorders. G. Jin, A. Aobulikasimu,J. Piao,Z. Aibibula, D. Koga, H. Ochi, K. Ishiyama, T.
Kanno, Yoshimi Niwano, Atsushi Okawa, Yoshinori Asou. Journal of medical and dental
sciences 2018 65 45-50
16. Hyperglycemia attenuates receptor activator of NF-κB ligand-induced macrophage activation
by suppressing insulin signaling. Kurihara C, Tanaka T, Yamanouchi D. J Surg Res. 2017
Jun 15;214:168-175. IF 2.051
17. Hyperglycemia Suppresses RANKL-Induced Osteoclast Differentiation through LXRβ
Expression in RAW264.7 Cells. Tanaka T, Takei Y, Zaima N, Moriyama T, Yamanouchi D.J
Nutr Sci Vitaminol (Tokyo). 2017;63(1):28-34. IF 0.952
18. Intraoperative evaluation of the effects of femoral component offset and head size on joint
stability in total hip arthroplasty. Jinno T, Koga D, Asou Y, Morita S, Okawa A, Muneta T. J
Orthop Surg (Hong Kong). 2017 Jan 1;25(1):2309499016684298. IF 0.994
19. Pleiotropic Functions of High Fat Diet in the Etiology of Osteoarthritis. Asou Y, Iwata M, Ochi
H, Ailixiding M, Aibibula Z, Piao J, Jin G, Hara Y, Okawa A. PLoS One. 2016 Sep
9;11(9):e0162794. IF 2.806
20. Osteoclastogenic Differentiation of Macrophages in the Development of Abdominal Aortic
Aneurysms. Takei Y, Tanaka T, Kent KC, Yamanouchi D. Arterioscler Thromb Vasc Biol.
2016 Sep;36(9):1962-71. IF 6.607
21. Puerarin Suppresses Macrophage Activation via Antioxidant Mechanisms in a CaPO4Induced Mouse Model of Aneurysm. Tanaka T, Moriyama T, Kawamura Y, Yamanouchi D. J
Nutr Sci Vitaminol (Tokyo). 2016;62(6):425-431. IF 0.952
22. Inter-rater agreement and checklist validation for postoperative wound assessment using
smartphone images in vascular surgery. Wiseman JT, Fernandes-Taylor S, Gunter R,
Barnes ML, Saunders RS, Rathouz PJ, Yamanouchi D, Kent KC. J Vasc Surg Venous
Lymphat Disord. 2016 Jul;4(3):320-328. IF 1.143
23. Hyperglycemia Suppresses Calcium Phosphate-Induced Aneurysm Formation ThroughInhibition of Macrophage Activation. Tanaka T, Takei Y, Yamanouchi D. J Am Heart Assoc.
2016 Mar 28;5(3) IF 4.425
24. Xanthine oxidoreductase activation is implicated in the onset of metabolic arthritis. Aibibula
Z, Ailixiding M, Iwata M, Piao J, Hara Y, Okawa A, Asou Y. Biochem Biophys Res
Commun. 2016 Mar 25;472(1):26-32. IF 2.466
25. Limited significance of screening computed tomography after cementless total hip arthroplasty
with highly cross-linked polyethylene at 7-10 years of follow-up. Takada R, Jinno T, Koga D,
Yamauchi Y, Asou Y, Muneta T, Okawa A. Mod Rheumatol. 2016 Feb 16:1-4.
26. Case Report of Percutaneous Retrograde Transcollateral Recanalization of the Superior
Mesenteric Artery via the Celiac Artery for Acute Mesenteric Ischemia. Gupta PK, Smith BK,
Yamanouchi D. Medicine (Baltimore). 2015 Dec;94(50):e2036. IF 2.133
27. MicroRNA-145 regulates osteoblastic differentiation by targeting the transcription factor Cbfb.
Fukuda T, Ochi H, Sunamura S, Haiden A, Bando W, Inose H, Okawa A, Asou Y, Takeda S.
FEBS Lett. 2015 Oct 24;589(21):3302-8. IF 3.169
28. Pivotal role of Sirt6 in the crosstalk among ageing, metabolic syndrome and osteoarthritis.
Ailixiding M, Aibibula Z, Iwata M, Piao J, Hara Y, Koga D, Okawa A, Morita S, Asou Y.
Biochem
BiophysRes
Commun.
2015
Oct23;466(3):319-26.
doi:
10.1016/j.bbrc.2015.09.019. IF 2.297
29. Mitochondrial superoxide in osteocytes perturbs canalicular networks in the setting of agerelated osteoporosis. Kobayashi K, Nojiri H, Saita Y, Morikawa D, Ozawa Y, Watanabe K,
Koike M, Asou Y, Shirasawa T, Yokote K, Kaneko K, Shimizu T. Scientific Reports. 2015
Mar 16;5:9148. IF 5.578
30. Enhancement of Runx2 expression is potentially linked to β-catenin accumulation in canine
intervertebral disc degeneration. Iwata M, Aikawa T, Hakozaki T, Arai K, Ochi H, Haro H,
Tagawa M, Asou Y, Hara Y. J Cell Physiol. 2014 Jun 11. IF 3.874
31. Tumor necrosis factor-α enhances RANKL expression in gingival epithelial cells via protein
kinase A signaling. Fujihara R, Usui M, Yamamoto G, Nishii K, Tsukamoto Y, Okamatsu Y,
Sato T, Asou Y, Nakashima K, Yamamoto M. J Periodontal Res. 2014 Aug;49(4):508-17 IF
2.215
32. Sirt6 regulates postnatal growth plate differentiation and proliferation via Ihh signaling. Piao
J, Tsuji K, Ochi H, Iwata M, Koga D, Okawa A, Morita S, Takeda S, Asou Y. Scientific
Reports. 2013 Oct 23;3:3022 IF 5.578
33. Current status of medical treatment for abdominal aortic aneurysm. Kurosawa K, Matsumura
JS, Yamanouchi D. Circ J. 2013;77(12):2860-6. Review. IF 3.685
34. Aneurysmal disease: the abdominal aorta. Takayama T, Yamanouchi D. Surg Clin North
Am. 2013 Aug;93(4):877-91. Review. IF 1.932
35. Cytoplasmic reactive oxygen species and SOD1 regulate bone mass during mechanicalunloading. Morikawa D, Nojiri H, Saita Y, Kobayashi K, Watanabe K, Ozawa Y, Koike M, Asou
Y, Takaku T, Kaneko K, Shimizu T. J Bone Miner Res. 2013 Nov;28(11):2368-80. IF 6.128
36. Variations in gene and protein expression in canine chondrodystrophic nucleus pulposus cells
following long-term three-dimensional culture. Iwata M, Ochi H, Asou Y, Haro H, Aikawa T,
Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y. PLoS One. 2013 May 2;8(5):e63120. IF 3.73
37. The United States StuDy for EvalUating EndovasculaR TreAtments of Lesions in the
Superficial Femoral Artery and Proximal Popliteal By usIng the Protégé EverfLex NitInol
STent SYstem II (DURABILITY II). Matsumura JS, Yamanouchi D, Goldstein JA, Pollock
CW, Bosiers M, Schultz GA, Scheinert D, Rocha-Singh KJ. J Vasc Surg. 2013 Jul;58(1):7383. IF 2.980
38. Sema3A regulates bone-mass accrual through sensory innervations. Fukuda T, Takeda S,
Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C,
Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H.
Nature. 2013 May 23;497(7450):490-3. IF 38.597

39. Initial responses of articular tissues in a murine high-fat diet-induced osteoarthritis model:
pivotal role of the IPFP as a cytokine fountain. Iwata M, Ochi H, Hara Y, Tagawa M, Koga D,
Okawa A, Asou Y. PLoS One. 2013 Apr 12;8(4) IF 3.73
40. Evaluation of the association between runt-related transcription factor 2 expression andintervertebral disk aging in dogs. Itoh H, Hara Y, Tagawa M, Kato T, Ochi H, Koga D, Okawa
A, Asou Y. Am J Vet Res. 2012 Oct;73(10):1553-9. IF 1.269
41. Balanced mineralization in the arterial system: possible role ofosteoclastogenesis/osteoblastogenesis in abdominal aortic aneurysm and stenotic disease.
Yamanouchi D, Takei Y, Komori K. Circ J. 2012;76(12):2732-7. Review. IF 3.578
42. Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly
developed calcium phosphate rodent abdominal aortic aneurysm model. Yamanouchi D,
Morgan S, Stair C, Seedial S, Lengfeld J, Kent KC, Liu B. J Vasc Surg. 2012
Aug;56(2):455-61. IF 2.879
43. Suwanabol PA, Seedial SM, Shi X, Zhang F, Yamanouchi D, Roenneburg D, Liu B, Kent
KC. Transforming growth factor-β increases vascular smooth muscle cell proliferation
through the Smad3 and extracellular signal-regulated kinase mitogen-activated protein
kinases pathways. J Vasc Surg. 2012 Aug;56(2):446-54. IF 2.879
44. Comparison of different distal designs of femoral components and their effects on bone
remodeling in 1-stage bilateral total hip arthroplasty. Yamauchi Y, Jinno T, Koga D, Asou Y,
Morita S, Okawa A. J Arthroplasty. 2012 Sep;27(8): IF 2.384
45. Runx2 haploinsufficiency ameliorates the development of ossification of the posterior
longitudinal ligament.Iwasaki M, Piao J, Kimura A, Sato S, Inose H, Ochi H, Asou Y,
Shinomiya K, Okawa A, Takeda S. PLoS ONE. 2012;7(8): IF 4.092
46. Procyanidin B3 prevents articular cartilage degeneration and heterotopic cartilage formation
in a mouse surgical osteoarthritis model. Aini H, Ochi H, Iwata M, Okawa A, Koga D, Okazaki
M, Sano A, Asou Y. PLoS ONE. 2012;7(5): IF 4.092
47. Osteoarthritis: Procyanidin B3 prevents OA in vivo. Ray K. Nature Revews Rheumatology.
2012 Jun 29;8(7):369. IF 8.388
48. Results of carotid artery stenting with distal embolic protection with improved systems:
Protected Carotid Artery Stenting in Patients at High Risk for Carotid Endarterectomy(PROTECT) trial. Matsumura JS, Gray W, Chaturvedi S, Yamanouchi D, Peng L, Verta P. J
Vasc Surg. 2012 Apr;55(4):968-976. IF 2.879
49. Delivery of plasmid DNA to vascular tissue in vivo using catheter balloons coated with
polyelectrolyte multilayers. Saurer EM, Yamanouchi D (Equal First Author), Liu B, Lynn DM.
Biomaterials. 2011 Jan;32(2):610-8. IF 7.404
50. Zhang F, Banker G, Liu X, Suwanabol PA, Lengfeld J, Yamanouchi D, Kent KC, Liu B. The
novel function of advanced glycation end products in regulation of MMP-9 production. J
Surg Res. 2011 Dec;171(2):871-6. IF 2.247
51. Osteopontin deficiency impairs wear debris-induced osteolysis via regulation of cytokine
secretion from murine macrophages. Shimizu S, Okuda N, Kato N, Rittling SR, Okawa A,
Shinomiya K, Muneta T, Denhardt DT, Noda M, Tsuji K, Asou Y. Arthritis Rheum. 2010
May;62(5):1329-37. IF 8.435
52. The roles of TNFR1 in lipopolysaccharide-induced bone loss: dual effects of TNFR1 on bonemetabolism via osteoclastogenesis and osteoblast survival. Ochi, H ;Hara, Y ;Tagawa,
M ;Shinomiya, K ;Asou, Y J Orthop Res. 2010 May;28(5):657-63. IF 2.976
53. Effects of caspase inhibitor on angiotensin II-induced abdominal aortic aneurysm inapolipoprotein E-deficient mice. Yamanouchi D, Morgan S, Kato K, Lengfeld J, Zhang F, Liu
B. Arterioscler Thromb Vasc Biol. 2010 Apr;30(4):702-7. IF 7.215
54. Protein kinase C delta mediates arterial injury responses through regulation of vascularsmooth muscle cell apoptosis. Yamanouchi D, Kato K, Ryer EJ, Zhang F, Liu B. CardiovascRes. 2010 Feb 1;85(3):434-43. IF 6.051

55. Caspase-mediated protein kinase C-delta cleavage is necessary for apoptosis of vascularsmooth muscle cells. Kato K, Yamanouchi D, Esbona K, Kamiya K, Zhang F, Kent KC, Liu
B. Am J Physiol Heart Circ Physiol. 2009 Dec;297(6):H2253-61. IF 3.712
56. Anti-receptor for advanced glycation end products therapies as novel treatment for
abdominal aortic aneurysm. Zhang F, Kent KC, Yamanouchi D, Zhang Y, Kato K, Tsai S,
Nowygrod R, Schmidt AM, Liu B. Ann Surg. 2009 Sep;250(3):416-23. IF 7.900
57. Arterial gene transfer of the TGF-beta signalling protein Smad3 induces adaptive
remodelling following angioplasty: a role for CTGF. Kundi R, Hollenbeck ST, Yamanouchi D,
Herman BC, Edlin R, Ryer EJ, Wang C, Tsai S, Liu B, Kent KC. Cardiovasc Res. 2009 Nov
1;84(2):326-35. IF 5.801
58. TGF-beta through Smad3 signaling stimulates vascular smooth muscle cell proliferation andneointimal formation. Tsai S, Hollenbeck ST, Ryer EJ, Edlin R, Yamanouchi D, Kundi R,
Wang C, Liu B, Kent KC. Am J Physiol Heart Circ Physiol. 2009 Aug;297(2):H540-9. IF
3.712
59. Therapeutic approach against intimal hyperplasia of vein grafts through endothelial nitricoxide synthase/nitric oxide (eNOS/NO) and the Rho/Rho-kinase pathway. Sugimoto M,
Yamanouchi D, Komori K. Surg Today. 2009;39(6):459-65. Review. IF 1.050
60. Transforming growth factor-beta promotes recruitment of bone marrow cells and bonemarrow-derived mesenchymal stem cells through stimulation of MCP-1 production in
vascular smooth muscle cells. Zhang F, Tsai S, Kato K, Yamanouchi D, Wang C, Rafii S, Liu
B, Kent KC. J Biol Chem. 2009 Jun 26;284(26):17564-74. IF 5.328
61. Characterization of primary and restenotic atherosclerotic plaque from the superficial
femoral artery: Potential role of Smad3 in regulation of SMC proliferation. Edlin RS, Tsai S,
Yamanouchi D, Wang C, Liu B, Kent KC. J Vasc Surg. 2009 May;49(5):1289-95. IF 3.517
62. Sarpogrelate hydrochloride reduced intimal hyperplasia in experimental rabbit vein graft.
Kodama A, Komori K, Hattori K, Yamanouchi D, Kajikuri J, Itoh T. J Vasc Surg. 2009
May;49(5):1272-81. IF 3.517
63. TNF-alpha-induced NF-kappaB signaling reverses age-related declines in VEGF induction
and angiogenic activity in intervertebral disc tissues. Ohba T, Haro H, Ando T, Wako M,
Suenaga F, Asou Y, Koyama K, Hamada Y, Nakao A. J Orthop Res. 2009 Feb;27(2):22935. IF 3.112
64. Effects of compressive loading on biomechanical properties of disc and peripheral tissue in a
rat tail model. Nakamura T, Iribe T, Asou Y, Miyairi H, Ikegami K, Takakuda K. Eur Spine J.
2009 Jun 26. IF 1.956
65. Biodegradable arginine-based poly(ester-amide) s as non-viral gene delivery reagents.
Yamanouchi D, Wu J, Lazar AN, Kent KC, Chu CC, Liu B. Biomaterials. 2008
Aug;29(22):3269-77. IF 6.646
66. Pitavastatin inhibits intimal hyperplasia in rabbit vein graft. Fujita H, Banno H, Yamanouchi
D, Kobayashi M, Yamamoto K, Komori K. J Surg Res. 2008 Aug;148(2):238-43. IF 1.875
67. The distinct role of the Runx proteins in chondrocyte differentiation and intervertebral discdegeneration: findings in murine models and in human disease. Sato S, Kimura A, Ozdemir
J, Asou Y, Takeda S. Arthritis Rheum. 2008 Sep;58(9):2764-75. IF 6.787
68. Effects of pore size and implant volume of porous hydroxyapatite/collagen (HAp/Col) on boneformation in a rabbit bone defect model. Tsuchiya A, Sotome S, Asou Y, Kikuchi M, Koyama
Y, Ogawa T, Tanaka J, Shinomiya K. J Med Dent Sci. 2008 Mar;55(1):91-9.
69. Itoh H, Asou Y, Hara Y, Haro H, Shinomiya K, Tagawa M.Enhanced type X collagen
expression in the extruded nucleus pulposus of the chondrodystrophoid dog.J Vet Med Sci.
2008 Jan;70(1):37-42. IF 1.573
70. Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of
nitric oxide function through an inhibition of superoxide production. Hattori K, Yamanouchi
D, Banno H, Kobayashi M, Yamamoto K, Kajikuri J, Itoh T, Komori K. J Vasc Surg. 2007

Jul;46(1):116-23. IF 1.836
71. Prevention of cartilage destruction with intraarticular osteoclastogenesis inhibitory
factor/osteoprotegerin in a murine model of osteoarthritis. Shimizu S, Asou Y, Itoh S, Chung
UI, Kawaguchi H, Shinomiya K, Muneta T. Arthritis and Rheumatism. 2007 Sep
28;56(10):3358-3365 IF7.677
72. ED-71, a novel vitamin D analog, promotes bone formation and angiogenesis and inhibitsbone resorption after bone marrow ablation. Okuda N, Takeda S, Shinomiya K, Muneta T,
Itoh S, Noda M, Asou Y. Bone. 2007 Feb;40(2):281-92. Epub 2006 Oct 16 IF 3.966
73. Enhancement of tissue engineered bone formation by a low pressure system improving cellseeding and medium perfusion into a porous scaffold. Wang J, Asou Y, Sekiya I, Sotome S,
Orii H, Shinomiya K Biomaterials. 2006 May;27(13):2738-46. IF 5.196
74. External iliac venous aneurysm in a pregnant woman: a case report. Banno H, Yamanouchi
D, Fujita H, Nagata J, Kobayashi M, Matsushita M, Nishikimi N, Komori K. J Vasc Surg.
2004 Jul;40(1):174-8. Review. IF 2.730
75. Coordinated expression of scleraxis and Sox9 genes during embryonic development oftendons and cartilage. Asou Y, Nifuji A, Tsuji K, Shinomiya K, Olson EN, Koopman P, Noda
M. J Orthop Res. 2002 Jul;20(4):827-33. IF 3.112
76. Bone metabolism and angiogenesis]. Noda M, Nomura K, Asou Y, Nemoto H, Ishijima M,
Takamoto M, Usui M, Kashimada K. Clin Calcium. 2001 Apr;11(4):404-10.
77. Osteopontin facilitates angiogenesis, accumulation of osteoclasts, and resorption in ectopic
bone. Asou Y, Rittling SR, Yoshitake H, Tsuji K, Shinomiya K, Nifuji A, Denhardt DT, Noda M.
Endocrinology. 2001 Mar;142(3):1325-32. IF 4.971
78. Transcription factors and osteoblasts. Noda M, Nifuji A, Tuji K, Furuya K, Ichiro S, Asou Y,
Kawaguchi N, Yamachita K. Front Biosci. 1998 Aug 1;3:d817-20. Review.