Data Sheet

Agilent NovoCyte Opteon
Spectral Flow Cytometer
Specifications
Instrument specifications
General Specifications
OpticsLaser

Solid state laser with onboard thermal-electric cooling with guaranteed
thermal stability				Beam Shaping Optics

Space coupled with minimal loss of laser power. Minimized stray laser-line
noise and minimized loss to reflection.			

Laser Beam Configuration

Spacially separated; fixed laser delay and no need for adjustment

Laser Operation

Only ON when acquiring samples. Significant extension of laser lifetime.

Photodetector

Avalanche photodiode (APD)

Optical Alignment ProcedureFixed. No operator alignment required.

Optical Filters

High-quality bandpass filters with optimized bandwidth for each detector

Forward Scatter (FSC)
Detection Laser

Off 488 nm

Side Scatter (SSC) Detection Laser

BSSC (488 nm Blue); VSSC (405 nm Violet)

Fluorescence Detection

Individual photodector for each channel. Solid state photodetector with highgain and photon detection efficiency. 			

FSC/SSC Sensitivity

FSC: 0.4 µm; VSSC: 0.08 µm; BSSC: 0.2 µm

Fluoresence Threshold Sensitivity– Fluorescein isothiocyanate (FITC) ≤ 50 molecules of equivalent soluble
fluorophores (MESF) (B543 channel)– Phycoerythrin (PE) ≤ 10 MESF (Y582 channel)– Allophycocyanin (APC) ≤ 10 MESF (R661 channel)– Pacific Blue ≤ 30 MESF (V455 channel)

Fluorescence Resolution< 3% CV for CEN

SSC and Fluorescence Light
Collection and Detection

Proprietary design of high numerical aperture objective lens with free-space
optical filters maximizing light collection efficiency

Fluidics
Flow Cell

170 x 490 μm rectangular quartz flow cell

Sample Acquisition Rate

100,000 events/second					Sample Delivery

Positive-displacement syringe pump enabling direct volumetric absolutecount without reference counting beads

Volumetric Absolute
Count Precision≤ 5%

Sample Flow Rate

5 to 120 µL/min in 1 μL increments				Sample Aquisition Volume1 μL to 5 mL

Sample Recovery Mode

Available

Compatibility to Autosampler

No fluidic tubing disassembly or reconnection required.

Fluid Level Sensing

Real-time active sensing with weight sensors. Automated warning when thefluid level is out of range.

Fluid Container Capacity

Sheath (6 L), waste (6 L), cleaning (500 mL), decontamination with automatic
liquid volume sensing (500 mL)
Optional large container for sheath (15 L) and waste (15 L)

Carryover<0.1%						Sample Injection Probe (SIP) Rinse

Automated flying collar wash of inner and outer SIP surface after eachsampling

Fluidics System Monitoring

In-line pressure sensor monitors the pressure in real time. Automated system
recovery when pressure is out of range due to clogging.

Fluidics System Maintenance– Automated startup and shutdown with fluidic system cleaning– Automated user executable cleaning, debubble, rinse, unclog, priming,and decontamination– Automated execution of user definable maintainence sequence

Signal Processing
Software

NovoExpress (Opteon) software

Parameters– Height and area for FSC, SSC, and all fluorescent channels– Width off FSC– TimeTriggering Channel

Threshold using any single channel or combination of any fluorescencechannel with FSC or SSC parameters.

Dynamic Range

24 bit; 7.2 decades logarithmic scale

Fluorescence Photodetector
Gain Control

Individually or proportionally adjustable. Optimized default gain for eachindividual channel.

Data Management12

Deconvolution Algorithm

Least square regression. Live unmixing during sample acquisition.

Compensation

Allow defining virtual filters and apply compensation

Autofluorescence Subtraction

Available

For custom configurations and for more information, please contact your local Agilent sales representative.

Output Data Files––––FCS 3.0
FCS 3.1
CSV
Batch PDF reports

Data Report

Automatic, customizable, batch

Workstation

Intel Core i9, 64G RAM, 256GB SSD, 16T HDD and above

Monitor27 inch flat panel (2,560 x 1,440 resolution) and above

Computer Operating System

Microsoft Windows 11 Professional (64 bit) and above

Usage Monitor

Comprehensive transaction log and system log

User ManagementAdministrative creation of individual user accounts and user groups. User
operation time tracking. 				Quality Control
Instrument Daily QC

Automatic daily quality control with QC particles. Levey-Jennings plot.

Production QC

Each instrument is verified for assembly integrity and performance tospecifications

Sampling
Manual Sample Loading

12 x 75 mm, 1.5 and 2.0 mL tube

Automatic Sample Loading withNovoSampler S

40 tube rack for 12 x 75 mm tube, 40 tube rack for microtubes, 24-, 48-, 96well (flat, V-, U-bottom), and 384-well microplates

Operating Conditions
Instrument Dimension– 33.9" W x 22.0" D x 19.3" H(86 x 56 x 49 cm)			
– 43.1" W x 24.4" D x 19.3" H(110 x 62 x 49 cm) with NovoSampler S

Instrument Weight (5 lasers)– 181 lb (82 kg)– 214 lb (97 kg) with NovoSampler S

Power Requirements

100 to 240 VAC, 50 to 60 Hz, 220 W

Operating
Environment Requirements– Temperature: 15 to 30 °C;– Relative humidity: 10 to 80%

Regulatory Compliance– Class 1 laser product– Reseach use only. Not for use in diagnostic or therapeutic procedures.
– SGS, UKCA, CE, KC, RCM, EACRegulatory Status

Laser combinations
The combination of lasers and filters is listed below. Refer to model for detailed
configurations.
Lasers and Fluorescence Channels

SystemFive Lasers

Four
LasersThreeLasers

Lasers349 nm(20 mW)

405 nm(130 mW)

488 nm(100 mW)

561 nm(100 mW)

637 nm(120 mW)

Channels

1918

14118

UVBYR

Number ofDetectors

73

UVBR

62

VBYR

54

URYB

54

UVYB

65

VBR

43

VYB

46

RYB

353

NovoSampler S specifications
General Specifications
Workstation
Operating System

Microsoft Windows 11 (64 bits)

SoftwareAgilent NovoExpress (Opteon) software 2.0 and above

Performance and Capability
Labware Compatibility

24 and 40 tube rack, 24-, 48-, 96-well (flat, U-, V-bottom), and 384-well
microplates

Labware Calibration

Automated calibration to accommodate different well depths. Calibrated
labware template can be saved for later use.

SIP Collision Detection

Automated detection of SIP collision and automated recovery. Automaticallyacquire the next sample after successful recovery.

Carryover

Rinse after sampling: < 0.1 %Mix Mode

Orbital shaking: Up to 3,000 rpm
Shaking diameter: 2 mm
User-definable mixing freqeuncy, speed, acceleration, and duration.

Barcode Reading

Integrated barcode reader. Automatically prompt barcode as specimen namein the software.

Fluidics System Rinse

Automated post-sampling rinse for every sample. User definable extra rinse
cycle and rinse frequency.

Sampling Overhead Volume

30 μL in absolute counting mode, 10 μL without absolute counting mode

Dead Volume– 96-well plate (flat bottom): 27 μL– 96-well plate (V bottom and U bottom): 1 μL
– 384-well plate: 3 μL

Installation
Installation Method

Easy attachment, user installableCalibration

Automated self-calibration after installation. No need to reconfigure fluidics
tubing or connection.

Operating Conditions
Dimension

19.3" W x 12.0" D x 12.0" H (49 x 30 x 30 cm)

Weight33 lb (15 kg)

Operaing Temperature

15 to 30 °C

Operating Humidity

Relative humidity: 10 to 80%

Power Requirements

12 V directly from NovoCyte Opteon flow cytometer

Regulatory Compliance
Regulationwww.agilent.com/lifesciences/novocyte

DE50568371
This information is subject to change without notice.
© Agilent Technologies, Inc. 2024
Printed in the USA, May 29, 2024
5994-7337EN

CE; research use only