ࣉࣟࢪ࢙ࢡࢺ◊✲඲యࡢᴫせ㔠ᒓ㓟໬≀⢏Ꮚ䛾೺ᗣᙳ㡪䛻㛵䛩䜛◊✲Toxicological study on ultrafine particles of metal oxides⋤ ⍞⏕*1 ೺ᗣ㞀ᐖண㜵◊✲ࢢ࣮ࣝࣉʄWANG, Rui-Sheng஧㓟໬ࢳࢱࣥ㸦TiO2㸧ࢼࣀ⢏Ꮚ㸦NPs㸧ࢆࡣࡌࡵ࡜ࡍࡿ྾ධᛶ㔠ᒓ㓟໬≀⢏Ꮚ≧≀㉁ࡢࣜࢫࢡホ౯ཬࡧࡑࢀ࡟ᇶ࡙ࡃつไ࣭⟶⌮ࡣປാ⾨⏕ୖ㔜せ࡞ㄢ㢟࡛࠶ࡿ㸬ࣜࢫࢡホ౯࡛ࡣ, ᠱᛕࡉࢀࡿ᭷ᐖᛶࡢ᭷↓ࢆ☜ㄆࡋ, ࡲࡓ, ⏝㔞཯ᛂᛶ࡟ᇶ࡙࠸࡚ࠕ㜈್ࠖࢆุ᩿ࡍࡿ㸬ᮏ◊✲ࡣࡇࡢホ౯࠾ࡼࡧᇶ‽್タᐃ࡟࢚ࣅࢹࣥࢫࢆᥦ౪ࡍࡿࡇ࡜ࢆ┠ⓗ࡜ࡍࡿ㸬௦⾲ⓗ࡞㔠ᒓ㓟໬≀⢏Ꮚ࡜ࡋ࡚Ⓨࡀࢇᛶࡀሗ࿌ࡉࢀ࡚࠸ࡿ TiO2 NPs ࢆᑐ㇟࡜ࡋ, ື≀ᐇ㦂࡟ࡼࡾ,⫵ࡸ⫢⮚࡞࡝ࡢ୺せ⮚ჾ࡟࠾ࡅࡿᙳ㡪ࢆ⅖⑕཯ᛂ࡜㑇ఏẘᛶ࡟↔Ⅼࢆ࠶࡚࡚ㄪ࡭ࡿ㸬ࡲࡓ, Ⓨࡀࢇᛶ࡜࡜ࡶ࡟ᠱᛕࡀ♧ࡉࢀ࡚࠸ࡿ⏕Ṫჾࡸ⚄⤒⣔࡬ࡢᙳ㡪ࢆ☜ㄆࡍࡿ㸬࣐࢘ࢫ࡟㸪NEDO ㄪᩚἲ࡛సᡂࡋࡓ୍ḟ⢏ᚄࡀ⣙ 21 nm ࡢ TiO2 NPs ࢆ㟼⬦ὀᑕࡲࡓࡣẼ⟶ෆᢞ୚ࡋࡓ࡜ࡇࢁ, 㸱࠿᭶ࡀ⤒㐣ࡋ࡚ࡶ⫢⮚ࡸ⫵࡟ṧ␃ࡋ࡚࠾ࡾ, ⣽⬊ෆ࡛ࡣ⣽⬊㉁࡟ᒁᅾࡋ࡚࠸ࡿࡇ࡜ࡀほᐹࡉࢀࡓ㸬ࡋ࠿ࡋ, ࡇࢀࡽࡢ⤌⧊ࡸ㦵㧊࡞࡝࡛ࡣ DNA ࡢ᪩ᮇᦆയࡸ㑇ఏᏊ✺↛ኚ␗࡞࡝ࡢ㑇ఏẘᛶࡣ᳨ฟࡉࢀ࡞࠿ࡗࡓ㸬୍᪉㸪NEDO ㄪ〇ἲ࡛స〇ࡋࡓヨᩱ࡛ࡣ, ⬻ࡸ⏕Ṫჾ࡬ࡢ᫂☜࡞⢏Ꮚࡢ⛣⾜ീࢆ☜ㄆࡍࡿࡇ࡜ࡀ࡛ࡁ࡞࠿ࡗࡓࡓࡵ, ᪂ࡓ࡟⫵⬊ࢧ࣮ࣇ࢓ࢡࢱࣥࢺࡸ⏕⌮㣗ሷỈ࡛ࡢศᩓ࣭ㄪ〇ἲࢆ☜❧ࡋ, ࡉࡽ࡟⺯ගᶆ㆑ࢆ⾜ࡗࡓ㸬ࡇࡢ᪉ἲ࡛ㄪ〇ࡋࡓヨᩱࢆ⏝࠸࡚ࣛࢵࢺ࡟Ẽ⟶ෆᢞ୚ࡋࡓ࡜ࡇࢁ, ⬻➼ࡢ⤌⧊࡟⢏Ꮚࡢ⛣⾜ീࡀ☜ㄆࡉࢀ, ࡲࡓ⣽⬊࣐࣮࣮࢝࡜ࡢే⏝࡛≉␗⣽⬊࡬ࡢ⢏Ꮚࡢྲྀࡾ㎸ࡳࢆ᳨ウࡍࡿࡇ࡜ࡀ࡛ࡁࡓ㸬⏕Ṫჾ࡬ࡢᙳ㡪࡛ࡣ, ⢭Ꮚ㐠ື⬟ࡸᡂ⇍ࡋࡓ⢭Ꮚᩘࡢ᭷ព࡞ῶᑡࡀ᳨ฟࡉࢀࡓ㸬ࡇࢀࡽࡢ⤖ᯝࡣ, TiO2 NPs ࡣ㛗ᮇ࡟ࢃࡓࡗ࡚⫵ࡸ⫢⮚࡞࡝ࡢ⤌⧊࡟ṧ␃ࡍࡿࡀ, 㑇ఏẘᛶࢆ♧ࡉࡎ, ⢭Ꮚ⏕ᡂ࡟ᙳ㡪ࢆ୚࠼ࡿࡇ࡜ࡀุ᫂ࡋࡓ㸬௒ᚋࠊ⢏Ꮚࢧ࢖ࢬ࡞࡝࡟ࡼࡿ⤌⧊࡜⣽⬊ෆືែࡢ㐪࠸ࡸ≉␗⣽⬊࡟࠾ࡅࡿᙳ㡪ࡢゎ᫂࡟ᮇᚅࡋࡓ࠸㸬1 ࡣࡌࡵ࡟㡪ࡀᠱᛕࡉࢀ࡚࠸ࡿ 3)㸬ࢼࣀࢧ࢖ࢬࡢ TiO2㸦10-50 nm㸧ࢆᐇ㦂ື≀㸦ࣛࢵࢺ㸧࡬៏ᛶⓗ࡟྾ධࡤࡃ㟢ࡍࡿ࡜, ⫵྾ධᛶ⢏Ꮚ≧≀㉁ࡢ⟶⌮ࡣປാ⾨⏕ୖ㔜せ࡞ㄢ㢟࡛࠶ࡀࢇࡀቑຍࡋࡓ࡜࠸࠺ሗ࿌ 6)ࡀ࠶ࡿࡓࡵ㸪ᅜ㝿ࡀࢇ◊✲ࡿࡀ, ཌ⏕ປാ┬࡟ࡼࡿ໬Ꮫ≀㉁ࡢࣜࢫࢡホ౯஦ᴗ࡛ࡣ,ᶵ㛵㸦IARC㸧ࡣ 2006 ᖺ࡟ TiO2 ࢆࢢ࣮ࣝࣉ 2B㸦ே࡟ᑐ஧㓟໬ࢳࢱࣥࡢࢼࣀ⢏Ꮚ➼ࢆᑐ㇟࡟, Ⓨࡀࢇᛶࡢሗ࿌➼ࡋ࡚Ⓨࡀࢇᛶࡢྍ⬟ᛶࡀ࠶ࡿ㸧࡟᱁ୖࡆࡋࡓ 7)㸬ࡇࡢⓎࢆ⪃៖ࡋ࡚, ࣜࢫࢡホ౯ࡀ⾜ࢃࢀࡿ㸬ホ౯࡟࠶ࡓࡗ࡚ࡣ,ࡀࢇస⏝ࡣ⫵࡬ࡢ㐣㈇Ⲵ࡟ࡼࡿ⫵ࡢ⅖⑕཯ᛂࡀⓎࡀࢇࡢᠱᛕࡉࢀࡿ᭷ᐖᛶࡢ᭷↓ࢆ☜ㄆࡍࡿᚲせࡀ࠶ࡿ࡜࡜ࡶ࡟,๓ᥦ࡜࡞ࡿ࡜ࡢ௬ㄝࡀ♧ࡉࢀ࡚࠸ࡿ 8)㸬ࡋ࠿ࡋ, Ⓨࡀࢇホ౯ᇶ‽࡜࡞ࡿࠕホ౯್ࠖࡢỴᐃࡢࡓࡵ࡟ࡣ᭷ᐖᛶⓎ⌧࡟࠸ࡓࡿྛẁ㝵࡟࠾ࡅࡿᶵᗎࡣᮍゎ࡛᫂࠶ࡿ㸬ࡲࡓࠊࣛᶵᗎࢆ⪃៖ࡍࡿࡇ࡜ࡀồࡵࡽࢀ࡚࠸ࡿ㸬㑇ఏẘᛶࡢ᭷↓ࢵࢺ࡬ࡢ㰯⭍ᢞ୚࠿ࡽ⢏Ꮚࡢ⬻࡬ࡢ⛣⾜ࡸ⬻ෆ࡟࠾ࡅࡿ࡟ᇶ࡙࠸࡚ࠕ㜈್ࠖࡢ᭷↓ࢆุ᩿ࡍࡿ࡜࠸ࡗࡓࡇ࡜ࡀ㔜⏕໬Ꮫⓗ࡞ኚ໬ࡀ⏕ࡌࡓ࡜ࡢሗ࿌ࡶ࠶ࡗࡓ 9,10)㸬ࡋ࠿ࡋ,せ࡜࡞ࡿ㸬ࡇࡢࡼ࠺࡞ุ᩿ࡢᇶ┙࡜࡞ࡿ᝟ሗࡀồࡵࡽࢀ⢏Ꮚࡢ⚄⤒⣔࡬ࡢ⛣⾜ࡢヲ⣽ࡸ⚄⤒⤌⧊ࡢᶵ⬟㞀ᐖ࡟ࡘ࡚࠸ࡿ㸬࠸࡚ࡢ᝟ሗࡣ୙༑ศ࡛࠶ࡿ㸬⢭Ꮚ⏕ᡂ࡞࡝⏕Ṫ⣔࡬ࡢᙳ஧㓟໬ࢳࢱࣥ㸦TiO2㸧⢏Ꮚࡣ௦⾲ⓗ࡞㔠ᒓ㓟໬≀⢏Ꮚ㡪࡟ࡘ࠸࡚ࡣṤ࡝ሗ࿌ࡉࢀ࡚࠸࡞࠸.

࡛࠶ࡾ, ⢏ᚄࢧ࢖ࢬࡀ 1-100 nm ࡢࡶࡢࡣᕤᴗ⏝ࢼࣀ࣐ᮏ◊✲ࡣ, ࡇࢀࡽࡢၥ㢟Ⅼ࡟ࡘ࠸࡚, ࣜࢫࢡホ౯ࡢᇶࢸࣜ࢔ࣝࡢࢼࣀ⢏Ꮚ㸦nanoparticles, NPs㸧ศ㢮࡟ᒓࡋ,┙࡜ࡍࡿࡇ࡜ࢆᛕ㢌࡟, ື≀ࢆ⏝࠸࡚ࡢࡤࡃ㟢ᐇ㦂ࢆ⾜ࡑࢀࡼࡾ኱ࡁ࠸ࡶࡢࡣᚤ⢏Ꮚ㸦fine particles, FPs㸧࡟࡞ࡗࡓ. ࡲࡓ, ≉␗⤌⧊࣭⣽⬊࡬ࡢ⛣⾜ࡸྲྀࡾ㎸ࡳࢆ㧗ឤࡿ 1,2) 㸬ࢼࣀ⢏Ꮚࡢ TiO2 ࡣ㔜せ࡞ᕤᴗᮦᩱ࡜ࡋ࡚, ໬⢝ᗘ࡟᳨ฟࡍࡿࡓࡵ, ᪂ࡓ࡞ヨᩱศᩓ࣭ᶆ㆑᪉ἲࢆ☜❧ࡋရ㸪ሬᩱ㸪㣗ရ㸪ගゐ፹࡞࡝ᖜᗈ࠸⏝㏵࡛฼⏝ࡉࢀ, ࡑࡓ㸬ྛᐇ㦂⤖ᯝࡢヲ⣽ࡣᮏᴫせ࡟⥆ࡃ✏ࢆཧ↷ࡉࢀࡓ࠸.

ࡢ౑⏝ࡀ௒ᚋ, ࡲࡍࡲࡍቑ኱ࡍࡿࡇ࡜ࡀண᝿ࡉࢀࡿ 3,4)㸬

௨ୗ࡛ࡣ୺࡞◊✲ෆᐜ࡜ᡂᯝ࡟ࡘ࠸࡚㏙࡭ࡿ.

୍⯡ⓗ࡟ TiO2 FPs ࡣẘᛶࡀప࠸࡜ᛮࢃࢀࡿ㸬୍᪉࡛,TiO2 NPs ࡣࢧ࢖ࢬࡀᑠࡉ࠸ୖ, ᙉ࠸ගゐ፹స⏝࡞࡝ࡢ2 ◊✲ࡢᴫせᛶ㉁ࢆᣢࡗ࡚࠸ࡿࡓࡵ, ࡑࡢࡤࡃ㟢࡟ࡼࡿே࡬ࡢ೺ᗣᙳ1) ẘᛶᐇ㦂ࡢࡓࡵࡢ஧㓟໬ࢳࢱࣥศᩓᾮࡢㄪ〇ἲࡢ᳨

*1 Mechanism of Health Effects Research Group,ウ

National Institute of Occupational Safety and Health, Japan－83－㸫㸫労働安全衛生総合研究所特別研究報告 JNIOSH-SRR-NO.45 (2015)
ປാᏳ඲⾨⏕⥲ྜ◊✲ᡤ≉ู◊✲ሗ࿌ࠉJNIOSH-SRR-NO.45

ᐇ㦂࡟౑⏝ࡍࡿ TiO2 NPs ヨᩱࡢศᩓ᪉ἲ࡟ࡘ࠸᳨࡚

䛷䛿⫵⤌⧊䛻 TiO2 ⢏Ꮚ䛾จ㞟య䛜ほᐹ䛥䜜䛯䠊୍᪉, ⫵ウࡋࡓ㸬ヨᩱࡣ P25㸦࢔ࢼࢱ࣮ࢮ࣭ࣝࢳࣝ⤖ᬗ┦ΰᅾᆺ⤌⧊䛻䛚䛡䜛㑇ఏᏊ✺↛ኚ␗㢖ᗘ䛾ୖ᪼䛿 TiO2 NPs ᢞTiO2㸧㸪ศᩓᾮࡣ㉸⣧Ỉ㸦UPW㸧㸪0.2%ࣜࣥ㓟Ỉ⣲஧ࢼ୚⩌࡛ࡣ≉࡟ㄆࡵࡽࢀ࡞࠿ࡗࡓ㸬䛣䜜䜙䛾⤖ᯝ䛛䜙䠈Ẽ⟶ࢺ࣒ࣜ࢘Ỉ㸦DSP㸧㸪ࢲࣝ࣋ࢵࢥࣜࣥ㓟⦆⾪⏕⌮㣗ሷỈෆᢞ୚䛥䜜䛯 TiO2 NPs 䛿䠈䠏䛛᭶ᚋ䜒⫵䛻ṧ␃䛧䛶䛚䜚䠈㸦PBS㸧㸪⏕⌮㣗ሷỈ㸦S㸧㸪0.05㸣⏺㠃άᛶ๣ῧຍ⏕⌮㍍ᚤ䛺⅖⑕䛜క䛳䛶䛔䜛䛣䛸䛜ุ᫂䛧䛯䠊㑇ఏẘᛶస⏝䛻㣗ሷỈ㸦ST㸧ࡢ 5 ✀㢮ࢆᑐ㇟࡜ࡋ࡚ྛ⤌ࡳྜࢃࡏࡢᠱ⃮䛴䛔䛶䛿䠈䜘䜚㛗ᮇ䛻䜟䛯䛳䛶ほᐹ䛩䜛ᚲせ䛜䛒䜛䠊ᾮࢆ㉸㡢Ἴฎ⌮࣭㐲ᚰศ㞳࡟ࡼࡗ࡚ᚓࡽࢀࡓୖ⃈ᾮࢆᅇ཰ࡋ㸪ືⓗගᩓ஘ἲ࡟ࡼࡿᩓ஘ᙉᗘศᕸࡢ4) ஧㓟໬ࢳࢱࣥࢼࣀ⢏Ꮚ࡟ࡼࡿ୰ᯡ⚄⤒⣔࡬ࡢᙳ㡪࡟ᐃࢆᐇ᪋ࡋࡓ㸬ࡲࡓ㉸㡢Ἴฎ⌮᫬㛫࠾ࡼࡧᐜ㔞ࡢ᭱㐺᮲௳㸪㛗ᮇⓗࡘ࠸࡚࡞Ᏻᐃᛶࢆ᳨ウࡋࡓ㸬ࡑࡢ⤖ᯝ࠿ࡽ㸪P25 ࡣ UPW ࠾ࡼTiO2 NPs 䛾୰ᯡ⚄⤒⣔䜈䛾ᙳ㡪䛻䛴䛔䛶䛿䠈㰯⭍ෆᢞ

ࡧ DSP ࡟࠾࠸࡚ᖹᆒ⢏ᚄ 70-80 nm ௜㏆࡛༢୍ࣆ࣮ࢡ࡜୚䛻䜘䜛⣽⬊㛫⛣⾜䜔⤒ẕయ䜀䛟㟢䛻䜘䜛⾑ᾮ⬻㛵㛛࡞ࡿᏳᐃⓗ࡞⢏ᚄศᕸࢆ♧ࡋࡓ. PBS㸪S ࠾ࡼࡧ ST ࡟(BBB)ᙧᡂ௨๓䛾⫾௘䛾⬻ෆ䜈䛾⛣⾜䛸⚄⤒ఏ㐩䜈䛾ᙳࣆ࣮ࢡࡀ୙᳨ฟ࠶ࡿ࠸ࡣ」ᩘࡢࣆ࣮ࢡࡀ᳨

㡪䜢☜ㄆ䛧䛯ᩥ⊩䛿䛒䜛. ᮏ◊✲䛷䛿 TiO2 NPs 䜢ᡂ⇍䝬ฟࡉࢀ, Ⰻዲ࡞⢏ᚄศᕸࡣᚓࡽࢀ࡞࠿ࡗࡓ㸬ࡲࡓ㸪㉸㡢䜴䝇䛻ᢞ୚䛧䛯ሙྜ, ⬻ෆ䜈䛾⛣⾜䜔⚄⤒ఏ㐩⣔䜈䛾ᙳ㡪Ἴฎ⌮᫬㛫࠾ࡼࡧᐜ㔞ࡢ᭱㐺᮲௳࡟ࡘ࠸࡚ࡣ㸪ᠱ⃮ᾮࢆ䛾᭷↓䛻䛴䛔䛶᳨ウ䛧䛯䠊୍ḟ⢏Ꮚ䛜 21 nm 䛾 TiO2 NP

ࡘ࠸࡚ࡣ,10 ml ࡢᐜ㔞㸪30 ศ㛫ࡢ㉸㡢Ἴฎ⌮ࢆ⾜࠸㸪㐲ᚰศ㞳ᚋ䜢䝬䜴䝇ᑿ㟼⬦䜘䜚 4 ᅇᢞ୚䛧䠈୍ᐃᮇ㛫ᚋ⬻䜢๓⬻䠈ᚋ⬻䠈ࡢୖ⃈ᾮࢆ⏝࠸ࡿ࡜㸪ㄪ〇 0 ᪥࠿ࡽ 90 ᪥ᚋ࡟⮳ࡿࡲ࡛୰⬻䠈ᘏ㧊䠈ᑠ⬻䛾䠑㒊఩䛻ศ䛡䛶䝰䝜䜰䝭䞁䛸䜰䝭䝜㓟䛾⃰ᗘ䜢ศᯒ䛧䛯䠊᭱⤊ᢞ୚ 3 ᪥ᚋ䛾⬻ෆ Ti ⃰ᗘ䛿, 㧗⏝㔞⩌࡯ࡰ୍ᐃࡢᏳᐃ࡞⢏Ꮚᚄࢆ♧ࡋࡓ㸬䛷䛿᭷ព䛻ୖ᪼䛧, TiO2 䛾⬻ෆ䜈䛾⛣⾜䛜♧၀䛥䜜䛯. ᢞ2) ஧㓟໬ࢳࢱࣥࢼࣀ⢏Ꮚࢆᑿ㟼⬦ᢞ୚ࡋࡓ࣐࢘ࢫ࡟࠾୚ᚋ 9 ᪥䛷䛿䠈䝧䞁䝊䞁⎔䜢᭷䛩䜛䜰䝭䝜㓟䛾⃰ᗘ䛿䛩䜉䛶ࡅࡿ㑇ఏẘᛶ࡟ࡘ࠸࡚䛾㒊఩䛷ᢞ୚㔞౫Ꮡⓗ䛻ῶᑡ䛧䛯䛜䠈ศᯞ㙐䜰䝭䝜㓟䛿䜋䛸⢏Ꮚ䝃䜲䝈䛜 100 nm ௨ୗ䛾 TiO2 NPs 䛿ẚ⾲㠃✚䛜኱䜣䛹ኚ໬䛜䛺䛛䛳䛯䠊䝰䝜䜰䝭䞁䛾䝗䞊䝟䝭䞁䜔䝜䝹䜶䝢䝛䝣䝸䛝䛔䛯䜑䠈⾲㠃άᛶ䛜ቑ኱䛧䠈⏕య䛻ᝏᙳ㡪䜢ཬ䜌䛩ྍ⬟䞁䛿ᑠ⬻䛷䛿ᢞ୚㔞౫Ꮡⓗ䛻ῶᑡ䛧䛯䛜䠈௚䛾㒊఩䛷䛿䜋ᛶ䛜ᠱᛕ䛥䜜䛶䛔䜛䠊䝷䝑䝖䛾྾ධ䜀䛟㟢ᚋ䚸⫵䛜䜣䛾Ⓨ⏕䛸䜣䛹ᙳ㡪䛿䛺䛛䛳䛯䠊ᢞ୚ᚋ 3 䛛᭶䛷䛿䜰䝭䝜㓟䛾⬻ෆ⃰䛜ୖ᪼䛧䛯䛸䛔䛖ሗ࿌䛜䛒䜚, 䛭䛾㑇ఏẘᛶ䞉Ⓨ䛜䜣ᛶ䛻ᑐᗘ䛿䜋䜌ᅇ᚟䛧䛯䠊䛣䜜䜙䛾⤖ᯝ䛛䜙䠈TiO2 NPs 䛾ᢞ୚䛻䛩䜛㛵ᚰ䛜㧗䛔䠊ᮏ◊✲䛷䛿䠈✀䚻䛾⤌⧊䛷䛾ኚ␗䛜ゎᯒ䜘䛳䛶䠈䜰䝭䝜㓟䛾ྲྀ䜚㎸䜏䛜୍᫬ⓗ䛻㜼ᐖ䛥䜜䛯䛜䠈䜰䝭䝜䛷䛝䜛䝖䝷䞁䝇䝆䜵䝙䝑䜽䝬䜴䝇䠄 gpt Delta 䝬䜴䝇䠅䛻䠈TiO2㓟䛚䜘䜃䝰䝜䜰䝭䞁䛾⚄⤒ఏ㐩⣔䜈䛾ᙳ㡪䛿㍍ᚤ䛷䛒䜛䛣䛸NPs 䜢ᑿ㟼⬦ᢞ୚䛧䠈ᮎᲈ⾑ཬ䜃⫢⮚䛻䛴䛔䛶ྛ✀㑇ఏ䛜♧၀䛥䜜䛯䠊ẘᛶ䛾ゎᯒ䜢⾜䛳䛯䠊ᑠ᰾䜢᭷䛩䜛⥙≧㉥⾑⌫䛾ฟ⌧㢖ᗘ䜔㉥⾑⌫⭷ୖ䛾ኚ␗䝬䞊䜹䞊䛷䛒䜛 Pig-a 㑇ఏᏊ✺↛ኚ␗䛾㢖ᗘ䛿䠈TiO2 NPs 䛾ᢞ୚䛻䜘䜛ኚ໬䛿ぢ䜙䜜䛺䛛䛳䛯䠊䜎䛯䠈⫢⮚䛻䛚䛔䛶䜒 DNA ᦆയᛶ䠈gpt 㑇ఏᏊ✺↛ኚ␗䠄Ⅼ✺↛ኚ␗䠅㢖ᗘཬ䜃 Spi-ኚ␗య䠄Ḟኻ✺↛ኚ␗䠅㢖ᗘ䛾᭷ព䛺ቑຍ䛿ぢ䜙䜜䛺䛛䛳䛯䠊IL6 䜔 TNFǂ 䛺䛹䛾⅖⑕䝬䞊䜹䞊䜢ゎᯒ䛧䛯䛜, ⫢⮚䛻䛚䛡䜛⅖⑕཯ᛂ䛿≉䛻ほᐹ䛥䜜䛺䛛䛳䛯㸬䛣䜜䜙䛾⤖ᯝ䛿䠈TiO2 NPs 䛿䠈㦵㧊ཬ䜃⫢⮚䛷䛿㑇ఏẘᛶ䜢♧䛥䛺䛔䛣䛸䜢♧၀䛧䛶䛔䜛䠊3) ஧㓟໬ࢳࢱࣥࢼࣀ⢏ᏊࡢẼ⟶ෆᢞ୚ᚋࡢ࣐࢘ࢫ⫵࡟࠾ࡅࡿᙳ㡪࡟ࡘ࠸࡚TiO2 NPs 䛿࿧྾ჾ⣔䜀䛟㟢ᚋ䠈⫵䛾ᛴᛶ⅖⑕཯ᛂ䜢ㄏ5) ஧㓟໬ࢳࢱࣥࢼࣀ⢏Ꮚࡢ⢭ᕢẘᛶ࡟ࡘ࠸࡚
TiO2 NPs 䛿⫢⮚䜔⫵䛺䛹䛾୺せ⮚ჾ䜈䛾㞀ᐖ䛻䛴䛔䛶䛾ሗ࿌䛜ᩓぢ䛥䜜䜛䜒䛾䛾䠈㞝ᛶ⏕Ṫᶵ⬟䜈䛾ᙳ㡪䛻㛵䛩䜛᝟ሗ䛿ஈ䛧䛔䠊ᮏ◊✲䛷䛿 TiO2 NPs 䛜⢭ᕢᶵ⬟䛻୚䛘䜛ᙳ㡪䜢䠈䝬䜴䝇䜢⏝䛔䛶᳨ウ䛧䛯. ㉸㡢Ἴฎ⌮䛷䝸䞁㓟஧䝘䝖䝸䜴䝮ᾮ䛻ศᩓ䛥䛫䛯 TiO2 NPs 䜢䝬䜴䝇䛻㐌 1 ᅇ䠈ྜィ4 ᅇᑿ㟼⬦ᢞ୚䛧䠈᭱⤊ᢞ୚ 9 ᪥ᚋ䛻⢭ᕢཬ䜃⢭ᕢୖయᑿ㒊䜢᦬ฟ䛧䛶⢭ᕢ䜈䛾ᙳ㡪䜢ゎᯒ䛧䛯䠊⢭ᕢᶵ⬟䛾ᣦᶆ䛸䛧䛶䛿䠈⢭Ꮚᩘཬ䜃⢭Ꮚ㐠ື⬟䜢⏝䛔䛯䠊䛺䛚⢭Ꮚ㐠ື⬟䛿䠈඲⢭Ꮚ୰䛾㐠ື⢭Ꮚᩘ䛾๭ྜ䠄motile percent: MP䠅ཬ䜃㐠ື⢭Ꮚ୰䛾Ⰻዲ⢭Ꮚ䛾๭ྜ䠄progressive percent:PP䠅䛾 2 䛴䛾䝟䝷䝯䞊䝍䞊䛻䛴䛔䛶᳨ウ䛧䛯䠊䛭䛾⤖ᯝ䠈Ⓨ䛩䜛䛣䛸䛜ື≀ᐇ㦂䛷ほᐹ䛥䜜䛶䛔䜛䠊䜎䛯䠈⫵䛻ධ䛳䛯TiO2 NPs 䛾ᢞ୚䛿⢭ᕢཬ䜃⢭ᕢୖయᑿ㒊䛸䜒䛻⢭Ꮚᩘ䜢⢏Ꮚ䛿᤼ฟ䜔௚䛾⮚ჾ䜈䛾⛣⾜䜒䛒䜛䛜䠈୍㒊䛿㛗ᮇ䛻䜟᭷ព䛻ῶᑡ䛥䛫䛯䠊䜎䛯䠈⢭ᕢୖయᑿ㒊䛛䜙㐟ฟ䛥䛫䛯ᡂ䛯䛳䛶⫵䛻␃䜎䜛䛣䛸䜒▱䜙䜜䛶䛔䜛䠊ᮏ◊✲䛷䛿 TiO2 NPs䛾Ẽ⟶ෆᢞ୚ᚋ䛻⫵⤌⧊䛻䛚䛡䜛⅖⑕స⏝䜔㑇ఏᏊ✺↛ኚ␗䛺䛹䛻䛴䛔䛶᳨ウ䛧䛯䠊TiO2 NPs 䜢㉸㡢Ἴ䛷⵨␃Ỉ䛻ศᩓ䛧䠈䝬䜴䝇䛻Ẽ⟶ෆᢞ୚䛧䛯䠊ᢞ୚ 3 ᪥ཬ䜃 90 ᪥ᚋ䝬䜴䝇䜢ゎ๗䛧, ⫵⤌⧊ Ti ᐃ㔞, ⑓⌮ほᐹ, ⫵⬊Ὑίᾮ⇍⢭Ꮚ䛾㐠ື⬟䛿䠈MP ཬ䜃 PP 䛾୧䝟䝷䝯䞊䝍䞊䛸䜒᭷ព䛻పୗ䛧䛯䠊䛣䜜䜙䛾⤖ᯝ䛛䜙䠈TiO2 NPs 䛜⢭ᕢᶵ⬟㞀ᐖ䜢ᘬ䛝㉳䛣䛩ྍ⬟ᛶ䛜䝬䜴䝇䜢⏝䛔䛶♧䛥䜜䛯䠊䛺䛚䚸ᮏᐇ㦂䛾⤖ᯝ䛾୍㒊䛿 Fundamental Toxicological Sciencesㄅ䛻ᥖ㍕䛥䜜䛯 11).

䠄BALF䠅ゎᯒ, 㑇ఏᏊⅬ✺↛ኚ␗䛺䛹䛾ゎᯒ䜢⾜䛳䛯䠊BALF ゎᯒ䜔⫵⑓⌮᳨ᰝ䛾⤖ᯝ䠈᭱⤊ᢞ୚ 90 ᪥ᚋ䛻㍍ᚤ䛺䛜䜙䜒⅖⑕཯ᛂ䛜ㄆ䜑䜙䜜, 䜎䛯ගᏛ䛚䜘䜃㟁Ꮚ㢧ᚤ㙾6) ஧㓟໬ࢳࢱࣥࢼࣀ⢏Ꮚࡢ୰ᯡ⚄⤒⣔࡬ࡢ⏕≀Ꮫⓗ⵳✚ᛶ࡟ࡘ࠸࡚

－84－㸫㸫

金属酸化物粒子の健康影響に関する研究㔠ᒓ㓟໬≀⢏Ꮚࡢ೺ᗣᙳ㡪࡟㛵ࡍࡿ◊✲TiO2 NPs 䛾యෆ䛻䛚䛡䜛ືែ࡞ࡽࡧ࡟⛣⾜㒊఩࡛ࡢཧ ⪃ ᩥ ⊩ᙳ㡪ࡣᮍゎ᫂࡞Ⅼࡀከ࠸㸬ᚑ᮶౑⏝ࡉࢀ࡚࠸ࡿ NEDO1)

EU. European Commission Recommendation on

ㄪ〇ἲ࡛స〇ࡋࡓ TiO2 NPs ヨᩱࢆ⏝࠸࡚⾜ࡗࡓື≀ᐇthe definition of nanomaterial.

㦂࡛ࡣ, ⬻, ⏕Ṫჾ࡬ࡢ᫂☜࡞⢏Ꮚࡢ⛣⾜ീࡢ☜ㄆࡣ࡛

http://osha.europa.eu/en/news/eu-european-commࡁ࡞࠿ࡗࡓ. ᮏ◊✲࡛ࡣ, ࿧྾࡟ࡼࡾ⫵ෆ࡟౵ධࡋࡓࢼ

issionrecommendation-on-the-definition-of-nanoࣀ⢏Ꮚࡀ, ⫵⾲㠃࡟ỿ╔ࡋ, ࡑࡇ࡛ࢧ࣮ࣇ࢓ࢡࢱࣥࢺࢆmaterial.

྾╔ࡍࡿ⏕⌮ⓗ࡞⌧㇟ࢆ฼⏝ࡋ, ࢼࣀ⢏Ꮚࡢ᪂つㄪ〇᪉2)analysis.

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࡚⢏Ꮚࡢศᩓ࣭ㄪ〇ࢆ⾜ࡗࡓᚋ, ⺯ග≀㉁ࢆຍ࠼࡚ᶆ㆑
ࡋࡓ㸬ࡇࡢࡼ࠺࡟ㄪ〇ࡋࡓ TiO2 NPs ࢆࣛࢵࢺ࡟Ẽ⟶ෆ

Riu J, Maroto A, Rius FX. Nanosensors inenvironmentalἲࢆ☜❧ࡍࡿ㸬⫵ࢧ࣮ࣇ࢓ࢡࢱࣥࢺࡸ⏕⌮㣗ሷỈࢆ⏝࠸3)

Kisin ER, Murray AR, Keane MJ, Shi XC,

ᢞ୚ࡋ, ⬻࡞࡝࡬ࡢ⛣⾜ࢆ⺯ග㢧ᚤ㙾ἲ࡛ゎᯒࡋࡓ㸬྾

Schwegler-Berry╔ࡋࡓ TiO2 NPs ࡀ, ✵Ẽ-⾑ᾮ㛵㛛ࢆ㏱㐣ࡋ࡚, ⬻⾑⟶

Castranova V, Wallace WE, Kagan VE, Shvedova

௜㏆ࡢ⚄⤒⣔⣽⬊࡟ྲྀ㎸ࡲࢀ࡚࠾ࡾ, ⬻ᐇ㉁࡟⛣⾜ࡍࡿAA. Single-walled carbon nanotubes: geno- andࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓ. ⣽⬊࡟ྲྀࡾ㎸ࡲࢀࡓ⢏Ꮚࡣࡑࡢ

cytotoxic effects in lung fibroblast V79 cells. JGorelikO,

Arepalli

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ᚋ࡝ࡢࡼ࠺࡞ᣲືࢆ♧ࡍ࠿, ࡲࡓ⢏Ꮚࡢ⵳✚ࡀ࠶ࡿ⣽⬊ࡲࡓࡣ⬻⤌⧊඲య࡟࡝ࡢࡼ࠺࡞ᙳ㡪ࡀฟࡿ࠿࡟ࡘ࠸࡚୙D,4)

Robertson TA, Sanchez WY, Roberts MS. Are
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applied to the skin? J Biomed Nanotechnol. 2010;3 ௒ᚋࡢᒎᮃ

6:452-468.
5)௦⾲ⓗ࡞㔠ᒓ㓟໬≀⢏Ꮚ࡛࠶ࡿ TiO2 NPs ࡟ࡘ࠸࡚,MaynardAD,

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ᐇ㦂ື≀ࢆ⏝࠸࡚ࡑࡢయෆ࡟࠾ࡅࡿ⤌⧊⛣⾜, ᵝࠎ࡞⮚ჾ࣭⤌⧊࡟࠾ࡅࡿẘᛶᙳ㡪࡞࡝ࢆホ౯ࡋࡓ㸬ࡉࡽ࡟ẘᛶ6)

LeeKP,

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◊✲࡟⏝࠸ࡿヨᩱࡢㄪ〇࣭ᶆ㆑᪉ἲ㸪Ᏻᐃᛶ㸪ศᩓ፹ࡢ

Pulmonary response of rats exposed to titanium⏕యᙳ㡪࡞࡝࡟ࡘ࠸࡚ࡶ᳨ウࢆ⾜ࡗࡓ㸬㑇ఏᏊᦆയ࡟ࡘdioxide (TiO2) by inhalation for two years. Toxicol࠸࡚ࡣᮏ◊✲᳨࡛ウࡋࡓ᮲௳ୗ࡛ࡣ᫂☜࡞స⏝ࡀ♧ࡉࢀ

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࡞࠿ࡗࡓࡇ࡜࠿ࡽ, 㑇ఏẘᛶࡣ࡞࠸࡜⪃࠼ࡽࢀࡿ㸬ࡋ࠿7)ࡋ, ⫵࡞࡝ࡢ⤌⧊࡟࠾࠸࡚ TiO2 NPs 䛿㛗ᮇ䛻䜟䛯䛳䛶⵳IARC. Cobalt in Hard Metals and Cobalt Sulfate,
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Phosphide

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␃䛧䛶䛔䜛䛣䛸䜔ᙅ䛔⅖⑕཯ᛂ䛜క䛳䛶䛔䜛䛣䛸䛛䜙, 䜘䜚㛗ᮇ䛻ほᐹ䛩䜛ᚲせᛶ䛜♧၀䛥䜜䛯㸬䜎䛯, ᫂☜䛺⢭ᕢẘᛶ8)

Participants IRSIW. The relevance of the rat lung䛜♧䛥䜜, 㞝ᛶ⏕Ṫᶵ⬟䜈䛾ᙳ㡪䜢ヲ⣽䛻᳨ウ䛩䜛ᚲせ䛜

response to particle overload for human risk

䛒䜛㸬TiO2 NPs 䛿ᢞ୚ᚋ, ⾑ᾮ⬻㛵㛛䜢㏻㐣䛩䜛䛣䛸䛜ึ

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䜑䛶᫂䜙䛛䛸䛺䜚, ௒ᚋ฿㐩䛧䛯ྛ✀⣽⬊䞉㒊఩䛻䛚䛔䛶䛹9)䛾䜘䛖䛺ᙳ㡪䜢ཬ䜌䛩䛛, ⯆࿡῝䛔㸬ᮏ◊✲䛛䜙ከ䛟䛾⤖ᯝ䛜ᚓ䜙䜜, TiO2 NPs ࡢࣜࢫࢡホ౯

Wang J, Liu Y, Jiao F, Lao F, Li W, Gu Y, Li Y, GeG,LiB,ཬࡧࡑࡢࡤࡃ㟢ᇶ‽್ࡢタᐃ࡟㈨ࡍࡿ࡜⪃࠼ࡽࢀࡿ. ୍C,translocation

Zhouandpotentialetal.

Time-dependent᪉࡛, ◊✲ࡢ㐣⛬࡛ TiO2 NPs ࡢ⏕యᙳ㡪࡟㛵ࡍࡿ᪂ࡓcentral nervous system by intranasally instilled࡞␲ၥ࣭ㄢ㢟ࡀᥦ㉳ࡉࢀ, ௒ᚋ, ஧㓟໬ࢳࢱࣥࢼࣀ⢏ᏊTiO(2) nanoparticles. Toxicology. 2008; 254:82-90.

ࡢࡤࡃ㟢࡟ࡼࡿ೺ᗣᙳ㡪ࡢࡉࡽ࡞ࡿゎ᫂࡟⧅ࡀࡗ࡚࠸ࡃ10) Wang J, Chen C, Liu Y, Jia F, Li W, Lao F, Lia Y,

impairmenton

Lia B, Ge C, Zhou G, et al. Potential neurologicalࡇ࡜ࢆᮇᚅࡋࡓ࠸.

lesion          4 ㅰ ㎡

after

nasal

instillationofTiO2nanoparticles in the anatase and rutile crystal
phases. Toxicol Lett. 2008; 183:72-80.

ᮏ◊✲ࣉࣟࢪ࢙ࢡࢺࡣ 3 ᖺ㛫࡟ࢃࡓࡗ࡚⾜ࢃࢀࡓࡀ㸪11) Miura N, Ohtani K, Hasegawa T,et al.

◊✲௦⾲⪅ࡣ᭱ึࡢ 2 ᖺࡣᐑᕝ᐀அẶ㸦⌧ᅾࠊᖇி኱ᏛHazardous་⒪ᢏ⾡Ꮫ㒊㸧࡛࠶ࡗࡓࡀ, ᭱ᚋࡢ 1 ᖺࡣ➹⪅ࡀົࡵࡓ㸬nanoparticles on testicular function in mice. Fundᮇ㛫୰, ᘏ࡭ 12 ྡࡢ◊✲ဨ࡜እ㒊ࡢඹྠ◊✲⪅ࡀ◊✲

Toxicol Sci. 2014; 1: 81-85.

࡟ཧຍࡋ, ◊✲┠ᶆࡢ㐩ᡂ࡟㈉⊩ࡋࡓ㸬ࡇࡢሙࢆ೉ࡾ࡚
ㅰពࢆ⾲ࡋࡓ࠸㸬－85－㸫㸫effectsoftitaniumdioxide労働安全衛生総合研究所特別研究報告
JNIOSH-SRR-NO.45 (2015)
ປാᏳ඲⾨⏕⥲ྜ◊✲ᡤ≉ู◊✲ሗ࿌ࠉJNIOSH-SRR-NO.45(2015)◊✲ᴗ⦼䝸䝇䝖
ㄢ㢟ྡ䠖㻌㔠ᒓ㓟໬≀⢏Ꮚ䛾೺ᗣ㞀ᐖ䛻㛵䛩䜛◊✲㻌㻌㻌ᖹᡂ㻞㻢ᖺᗘ䠄㻞㻜㻝㻠ᖺ䠅

㻝

ཎⴭㄽᩥ

Nobuhiko Miura, Katsumi Ohtani, Tatusya Hasegawa, Rieko Hojo, Yukie Yanagiba, TetsuyaSuzuki, Megumi Suda, Rui-Sheng Wang (2014) Hazardous effects of titanium dioxide
nanoparticles on testicular function in mice. Fund Toxicol Sci, 1, 81-84.

㻞ᅜෆእ䛾◊✲㞟఍Ⓨ⾲୕ᾆఙᙪ䠈኱㇂຾ᕫ䠈㕥ᮌဴ▮䠈ᰗሙ⏤⤮䠈໭ᲄ⌮ᜨᏊ䠈㡲⏣ ᜨ䠈⋤ ⍞⏕(2014)䝏䝍䞁䝘䝜⢏ᏊP25䛜♧䛩⢭ᕢᶵ⬟㞀ᐖ䠈䝣䜷䞊䝷䝮2014⾨⏕⸆Ꮫ䞉⎔ቃ䝖䜻䝅䝁䝻䝆䞊䠈ㅮ₇せ᪨㞟䠈p231

㻟

ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Rui-Sheng Wang, Tetsuya Suzuki, Nobuhiko Miura, Rieko Hojo, Yukie Yanagiba, Megumi Suda
䠄2014)䚷Assessment of the Genotoxicity of TIO2 nanoparticles with gpt Delta transgenic mice.
NanoCon 2014 6th International Conference, Conference Proceedings, p153.

㻠ᅜෆእ䛾◊✲㞟఍Ⓨ⾲㛗㇂ᕝஓ㡲Ꮚ, ஂಖ⏣ஂ௦, ᑠᯘ೺୍, ᐑᕝ᐀அ䠄2014䠅஧㓟໬䝏䝍䞁䝘䝜⢏Ꮚ䛾䝷䝑䝖䜈䛾⏕యᙳ㡪䠄䠍䠅-ගᏛ㢧ᚤ㙾䛻䜘䜛᳨⣴-.䚷➨87ᅇ᪥ᮏ⏘ᴗ⾨⏕Ꮫ఍, ⏘ᴗ⾨⏕Ꮫ఍ㄅ 56ᕳ348
䝨䞊䝆.

㻡ᅜෆእ䛾◊✲㞟఍Ⓨ⾲ஂಖ⏣ஂ௦, 㛗㇂ᕝஓ㡲Ꮚ, ᑠᯘ೺୍, ᐑᕝ᐀அ䠄2014䠅஧㓟໬䝏䝍䞁䝘䝜⢏Ꮚ䛾䝷䝑䝖䜈䛾⏕యᙳ㡪䠄䠍䠅-㟁Ꮚ㢧ᚤ㙾䛻䜘䜛᳨⣴-.䚷➨87ᅇ᪥ᮏ⏘ᴗ⾨⏕Ꮫ఍, ⏘ᴗ⾨⏕Ꮫ఍ㄅ 56ᕳ348
䝨䞊䝆.

㻢ᅜෆእ䛾◊✲㞟఍Ⓨ⾲㛗㇂ᕝஓ㡲Ꮚ䠈ஂಖ⏣ஂ௦䠈ᑠᯘ೺୍䠈ᐑᕝ᐀அ䠄2014䠅䝷䝑䝖䛻䛚䛡䜛஧㓟໬䝏䝍䞁䝘䝜⢏Ꮚ䛾཯᚟㟼⬦ෆᢞ୚䛻䜘䜛⫢⮚䜈䛾ᙳ㡪䠊➨157ᅇ᪥ᮏ⋇་Ꮫ఍Ꮫ⾡㞟఍, ㅮ₇せ᪨㞟, p349.

㻣ᅜෆእ䛾◊✲㞟఍Ⓨ⾲㕥ᮌ೺୍㑻䠄2014䠅䝘䝜䝬䝔䝸䜰䝹䛾⾑ᾮ⬻㛵㛛㏱㐣ᛶ䛻㛵䛩䜛◊✲.䚷➨19ᅇປാᏳ඲⾨⏕⥲ྜ◊✲ᡤ䠉⏘ᴗ⏕ែ⛉Ꮫ◊✲ᡤ◊✲஺ὶ఍.

ᖹᡂ㻞㻡ᖺᗘ䠄㻞㻜㻝㻟ᖺ䠅㻝ᅜෆእ䛾◊✲㞟఍Ⓨ⾲ᑠᯘ೺୍䠈㛗㇂ᕝஓ㡲Ꮚ䠈ஂಖ⏣ஂ௦䠈ᐑᕝ᐀அ䠄2013䠅᭷ᐖᛶホ౯䛾䛯䜑䛾㓟໬䝏䝍䞁䝘䝜⢏Ꮚศᩓᾮ䛾ㄪ〇ἲ䛾᳨ウ 䠉⢏Ꮚᚄ䛾ゎᯒ䛚䜘䜃䝸䞁㓟Ỉ⣲஧䝘䝖䝸䜴䝮Ỉ䛾ẘᛶ䠉䠊➨86ᅇ᪥ᮏ⏘ᴗ⾨⏕Ꮫ఍䠈⏘ᴗ⾨⏕Ꮫ㞧ㄅ 55䠄Suppl.䠅䠈p364䠊

㻞ᅜෆእ䛾◊✲㞟఍Ⓨ⾲㛗㇂ᕝஓ㡲Ꮚ䠈ஂಖ⏣ஂ௦䠈ᑠᯘ೺୍䠈ᐑᕝ᐀அ䠄2013䠅㞝䝷䝑䝖䛻䛚䛡䜛஧㓟໬䝏䝍䞁䝘䝜⢏Ꮚ䛾཯᚟㟼⬦ෆᢞ୚䛻䜘䜛ᙳ㡪䠊➨156ᅇ᪥ᮏ⋇་Ꮫ఍Ꮫ⾡㞟఍䠈ㅮ₇せ᪨㞟䠈p224.

㻟ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Tetsuya Suzuki et al. (2013)䚷The in vitro toxicity of stably dispersed and large agglomerated
titanium dioxide nanoparticles. The 6th International Symposium on Nanotechnology,Occupational and Environmental Health, Abstracts, 85.

㻠ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Nobuhiko Miura et al, Investigation of adverse effect of nanosized titanium dioxide on testicularfunction in mice. The 6th International Symposium on Nanotechnology, Occupational andEnvironmental Health, Abstracts, 78.

㻡ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Rui-Sheng Wang et al, (2013) Analysis of the genotoxic effect of nanosized TiO2 asintravenously administered in mice. The 6th International Symposium on Nanotechnology,
Occupational and Environmental Health, Abstracts, 72.

－86－㸫㸫

金属酸化物粒子の健康影響に関する研究㔠ᒓ㓟໬≀⢏Ꮚࡢ೺ᗣᙳ㡪࡟㛵ࡍࡿ◊✲

㻢

㻣

㻤

㻥

ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Kenichi Kobayashi, Yasuko Hasegawa-Baba, Hisayo Kubota, Muneyuki Miyagawa (2013)
Optimized sample preparation of nanosized titanium dioxide for in vivo toxicological studies.
The 6th International Symposium on Nanotechnology, Occupational and Environmental Health,Abstracts, p61.

ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Hisayo Kubota, Yasuko Hasegawa-Baba, Kenichi Kobayashi, Muneyuki Miyagawa (2013)
Effects of nanosized titanium dioxide particles after repeated intravenous injections in adult rats.
The 6th International Symposium on Nanotechnology, Occupational and Environmental Health,
Abstracts, p 76.

ᅜෆእ䛾◊✲㞟఍Ⓨ⾲Yasuko Hasegawa-Baba, Hisayo Kubota, Kenichi Kobayashi, Muneyuki Miyagawa (2013)
Effects of nanosized TiO2 after repeated intravenous injections in pregnant rats. The 6thInternational Symposium on Nanotechnology, Occupational and Environmental Health,Abstracts, p 78.

ᅜෆእ䛾◊✲㞟఍Ⓨ⾲

Nobuhiko Miura, Katsumi Ohtani, Tetsuya Suzuki, Yukie Yanagiba, Rieko Hojo, Megumi Suda,
Muneyuki Miyagawa and Rui-Sheng Wang (2013) Adverse Effect of Nanosized Titanium
Dioxide on Testicular Function in Mice, The XIII International Congress of Toxicology, AbstractBook䠈p. 250.

ᖹᡂ㻞㻠ᖺᗘ䠄㻞㻜㻝㻞ᖺ䠅㻝ᅜෆእ䛾◊✲㞟఍Ⓨ⾲ᑠᯘ೺୍䠈㛗㇂ᕝஓ㡲Ꮚ䠈ஂಖ⏣ஂ௦䠈໭ᲄ⌮ᜨᏊ䠈ᐑᕝ᐀அ(2013) ᭷ᐖᛶホ౯䛾䛯䜑䛾
TiO2ศᩓᾮ䛾ㄪ〇ἲ(1) 䠉⢏Ꮚᚄ䛾ゎᯒ䠉.䚷➨83ᅇ᪥ᮏ⾨⏕Ꮫ఍Ꮫ⾡⥲఍ㅮ₇㞟䠈S211.

㻞ᅜෆእ䛾◊✲㞟఍Ⓨ⾲ᐑᕝ᐀அ䠈ஂಖ⏣ஂ௦䠈㛗㇂ᕝஓ㡲Ꮚ䠈ᑠᯘ೺୍(2013) ᭷ᐖᛶホ౯䛾䛯䜑䛾TiO2䝘䝜⢏Ꮚศᩓᾮ䛾ㄪ〇ἲ(2) 䠉䝸䞁㓟Ỉ⣲஧䝘䝖䝸䜴䝮Ỉ䛾ẘᛶ䠉.䚷➨83ᅇ᪥ᮏ⾨⏕Ꮫ఍Ꮫ⾡⥲఍ㅮ
₇㞟䠈S212.

－87－㸫㸫