ホ౯఍㆟ሗ࿌᭩

In vitro⭷ࣂࣜ࢔ヨ㦂ࢆ⏝࠸ࡓ⓶⭵⭉㣗ᛶヨ㦂௦᭰ἲ

JaCVAM ホ౯఍㆟

ᖹᡂ 29 ᖺ㸦2017 ᖺ㸧7 ᭶ 25 ᪥11JaCVAM ホ౯఍㆟኱ 㔝 Ὀ 㞝 㸦බ┈㈈ᅋἲே ᮌཎグᛕᶓ὾⏕࿨⛉Ꮫ᣺⯆㈈ᅋ㸧㸸ᗙ㛗㣤 ሯ ᑦ ᩥ 㸦⊂❧⾜ᨻἲே ་⸆ရ་⒪ᶵჾ⥲ྜᶵᵓ㸧*஬༑ᔒⰋ᫂ 㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ ⏕ά⾨⏕໬Ꮫ㒊㸧▼ ஭ 㞝 ஧ 㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ Ᏻ඲ᛶ⏕≀ヨ㦂◊✲ࢭࣥࢱ࣮㸧஭ ୖ ᬛ ᙲ 㸦᪥ᮏච␿ẘᛶᏛ఍㸧௒ ஭ ᩍ Ᏻ 㸦᪥ᮏື≀ᐇ㦂௦᭰ἲᏛ఍㸧ᒾ℩⿱⨾Ꮚ 㸦᪥ᮏ〇⸆ᕤᴗ༠఍㸧⠛ ⏣ ࿴ ಇ 㸦⊂❧⾜ᨻἲே ་⸆ရ་⒪ᶵჾ⥲ྜᶵᵓ㸧ᮡᒣ┿⌮Ꮚ 㸦᪥ᮏ໬⢝ရᕤᴗ㐃ྜ఍㸧௰ ஭ ಇ ྖ 㸦᪥ᮏ໬Ꮫᕤᴗ༠఍㸧୰ᮧࡿࡾࡇ 㸦⊂❧⾜ᨻἲே 〇ရホ౯ᢏ⾡ᇶ┙ᶵᵓ㸧す ᕝ ⛅ ె 㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ Ᏻ඲ᛶ⏕≀ヨ㦂◊✲ࢭࣥࢱ࣮㸧἟ ⃝  ⪽ 㸦᪥ᮏẘᛶᏛ఍㸧㔝 ཱྀ ┿ ᕼ 㸦⊂❧⾜ᨻἲே ་⸆ရ་⒪ᶵჾ⥲ྜᶵᵓ㸧**᳃ ⏣  ೺ 㸦᪥ᮏ⎔ቃኚ␗ཎᏛ఍㸧ᶓ 㛵 ༤ 㞝 㸦᪥ᮏ⓶⭵࢔ࣞࣝࢠ࣮࣭᥋ゐ⓶⭵⅖Ꮫ఍㸧                ௵ᮇ㸸ᖹᡂ 28 ᖺ 4 ᭶ 1 ᪥㹼ᖹᡂ 30 ᖺ 3 ᭶ 31 ᪥*㸸ᖹᡂ 28 ᖺ 4 ᭶ 1 ᪥㹼ᖹᡂ 29 ᖺ 3 ᭶ 31 ᪥**㸸ᖹᡂ 29 ᖺ 4 ᭶ 1 ᪥㹼ᖹᡂ 30 ᖺ 3 ᭶ 31 ᪥22

In vitro ⭷ࣂࣜ࢔ヨ㦂ࡣࠊ࢘ࢧࢠࢆ⏝࠸ࡿ⓶⭵⭉㣗ᛶヨ㦂ࡢ௦᭰࡜ࡋ࡚㛤Ⓨࡉࢀࡓヨ㦂ἲ࡛࠶ࡿࠋᮏヨ㦂ἲ࡛ࡣࠊ⭉㣗ᛶ≀㉁࡟ࡼࡾᘬࡁ㉳ࡇࡉࢀࡿ⭷ࣂࣜ࢔㞀ᐖࢆᣦᶆ࡜ࡋ࡚⓶⭵⭉㣗ᛶࢆホ౯ࡍࡿࠋᮏヨ㦂ἲ࡟ࡘ࠸࡚ࡣࠊḢ⡿࡛ࣂࣜࢹ࣮ࢩࣙࣥ◊✲ࡀᐇ᪋ࡉࢀࠊḢᕞ࡛ࡣ໬Ꮫ≀㉁ࡢ⓶⭵⭉㣗ᛶホ౯ࢆ┠ⓗ࡜ࡋ࡚ᢎㄆࡉࢀࠊ໬Ꮫ≀㉁ࡢ᭷ᐖᛶࡢศ㢮࣭⾲♧➼ࡢࡓࡵࡢヨ㦂ἲ࡜ࡋ࡚᥇⏝ࡉࢀ࡚࠸ࡿࠋࡲࡓࠊᮏヨ㦂ἲࡣ OECD㸦Organisation for Economic Co-operation and Development㸸⤒῭༠ຊ㛤Ⓨᶵᵓ㸧࡟࡚ࢸࢫࢺ࢞࢖ࢻࣛ࢖ࣥ㸦TG㸧435 ࡜ࡋ࡚ᢎㄆࡉࢀ࡚࠸ࡿ 1)ࠋ
JaCVAM ホ౯఍㆟ࡣࠊ⓶⭵⭉㣗ᛶヨ㦂㈨ᩱ⦅⧩ጤဨ఍࡟ࡼࡾసᡂࡉࢀࡓࠕIn vitro ⭷ࣂࣜ࢔ヨ㦂ࢆ⏝࠸ࡓ⓶⭵⭉㣗ᛶヨ㦂௦᭰ἲࡢホ౯ሗ࿌᭩ࠖ2)ࢆ⏝࠸࡚ࠊᮏヨ㦂ἲࡢጇᙜᛶ࡟ࡘ࠸᳨࡚ウࡋࡓࠋ㸯㸬ヨ㦂ἲࡢᐃ⩏ྡ⛠㸸 In vitro ⭷ࣂࣜ࢔ヨ㦂ࢆ⏝࠸ࡓ⓶⭵⭉㣗ᛶヨ㦂௦᭰ἲ௦᭰ࡍࡿᑐ㇟ẘᛶヨ㦂㸸 ࢘ࢧࢠࢆ⏝࠸ࡿ⓶⭵⭉㣗ᛶヨ㦂ヨ㦂ἲࡢᴫ␎㸸ᮏヨ㦂ἲ࡛ࡣࠊ࢘ࢧࢠ⓶⭵ࡢ௦ࢃࡾ࡟ྜᡂࡢ㧗ศᏊ࡛ᵓᡂࡉࢀࡿேᕤࡢ⭷ࣂࣜ࢔㸦invitro ⭷ࣂࣜ࢔㸧ࢆ⏝࠸ࠊ໬Ꮫ≀㉁࡟ࡼࡿ⓶⭵⭉㣗ᛶⓎ⌧ᶵᵓࡢ㔜せ࡞ࢫࢸࢵࣉ࡛࠶ࡿ⓶⭵ࡢࣂࣜ࢔ᶵ⬟ࡢ㞀ᐖࢆࠊ໬Ꮫ≀㉁᳨▱ࢩࢫࢸ࣒㸦Chemical Detection System㸸CDS㸧࡟ࡼࡗ᳨࡚ฟࡍࡿࡇ࡜࡟ࡼࡾࠊ⿕㦂≀㉁ࡢ⓶⭵⭉㣗ᛶࢆホ౯ࡍࡿࠋ⿕㦂≀㉁ࢆ in vitro⭷ࣂࣜ࢔ࡢ⾲㠃࡟㐺⏝ࡍࡿ࡜ࠊ⭉㣗ᛶࡢ⿕㦂≀㉁ࡀ⓶⭵࡟స⏝ࡍࡿᶵᗎ࡜ྠᵝ࡟ࡋ࡚ᘬࡁ㉳ࡇࡍ⭷ࣂࣜ࢔ࡢᦆയ࡟ࡼࡾࠊ⿕㦂≀㉁ࡣ⭷ࣂࣜ࢔ࢆᾐ㏱࣭㏻㐣ࡋ࡚ CDS ഃ࡟฿㐩ࡋࠊpH ࢖ࣥࢪࢣ࣮ࢱ࣮ࡢⰍࡢኚ໬ࡲࡓࡣ࢖ࣥࢪࢣ࣮ࢱ࣮ᾮࡢ௚ࡢ≉ᛶࡢኚ໬ࢆᘬࡁ㉳ࡇࡍ㸦໬Ꮫ཯ᛂࠊ㟁Ẽ໬Ꮫ཯ᛂ࡞࡝㸧ࠋ⭉㣗ᛶࡢ⛬ᗘࡣࠊ᭚㟢᫬㛫࡜ほᐹᮇ㛫࡟࠾ࡅࡿ CDS ࡢኚ໬ࡢ⛬ᗘ࠿ࡽุᐃࡍࡿࠋᮏヨ㦂ἲࡢࢩࢫࢸ࣒ࡣၟᴗⓗ࡟ࡣCorrositex®࡜ࡋ࡚㈍኎ࡉࢀ࡚࠸ࡿࠋ㸰㸬ホ౯࡟⏝࠸ࡓ㈨ᩱཬࡧホ౯ෆᐜࡢ⛉Ꮫⓗጇᙜᛶᮏヨ㦂ἲࡣࠊ⭉㣗ᛶࡢ⿕㦂≀㉁ࡀ⏕ࡁࡓ⓶⭵࡟స⏝ࡍࡿ㝿࡟ᘬࡁ㉳ࡇࡉࢀࡿ⭷ࣂࣜ࢔ᦆയࢆࠊேᕤࡢ⭷ࢆ⏝࠸࡚ホ౯ࡍࡿࡶࡢ࡛࠶ࡾࠊ⓶⭵⭉㣗ᛶⓎ⌧ᶵᗎࡀཎ⌮ⓗ࡟⪃៖ࡉࢀ࡚࠸ࡿࠋ
Corrositex®ἲࡣࠊECVAM㸦European Centre for the Validation of Alternative Methods㸸Ḣᕞ௦᭰ἲホ౯ࢭࣥࢱ࣮㸧࡟ࡼࡿࣂࣜࢹ࣮ࢩࣙࣥ◊✲ࡀ࡞ࡉࢀ 3,4,5)ࠊESAC㸦ECVAM Scientific Advisory Committee㸸ECVAM⛉Ꮫㅎၥጤဨ఍㸧࡟ࡼࡾಙ㢗ᛶ࡜෌⌧ᛶࡀ㧗࠸࡜ホ౯ࡉࢀ࡚࠸ࡿ 6)ࠋࡇࡢ⤖ㄽࡣࠊ⡿ᅜࡢ௦᭰ἲ࡟㛵ࡍࡿ┬ᗇ㛫㐃⤡఍㆟㸦ICCVAM㸸Interagency Coordinating Committee on the Validation of Alternative Methods)࡟࠾࠸࡚ࡶ☜ㄆࡉࢀ࡚࠸ࡿ 7,8)ࠋࡇࢀࡽࡢ⤖ᯝࡣබ⾲ࡉࢀ࡚࠾ࡾࠊ㏱࡛᫂⊂❧࡞⛉Ꮫⓗホ౯ࡀ⾜ࢃࢀ࡚࠸ࡿ࡜⪃࠼ࡿࠋ᭦࡟ࠊᮏヨ㦂ἲࡣࠊOECD ࡟࠾࠸࡚ࡶホ౯ࡉࢀࠊTG435 ࡜ࡋ࡚ᢎㄆࡉࢀ࡚࠸ࡿ 1)ࠋᮏ㑥࡟33࠾࠸࡚ࡣࠊJaCVAM ⓶⭵⭉㣗ᛶヨ㦂㈨ᩱ⦅⧩ጤဨ఍ࡀࠊࡇࢀࡽࡢ㈨ᩱࢆ⏝࠸࡚ᮏヨ㦂ἲࢆホ౯ࡋ࡚࠸ࡿ2)

ࠋ௨ୖࡢⅬ࠿ࡽࠊCorrositex®ἲࡣ⓶⭵⭉㣗ᛶࢆホ౯ࡍࡿ᪉ἲ࡜ࡋ࡚ཎ⌮ⓗጇᙜᛶࡀ࠶ࡾࠊࡲࡓࠊࡑࡢホ౯࡟⏝࠸ࡓ㈨ᩱ࡞ࡽࡧ࡟ホ౯ෆᐜ࡟ࡘ࠸࡚ࡣࠊ⛉Ꮫⓗጇᙜᛶࡀ࠶ࡿ࡜⪃࠼ࡿࠋ㸱㸬ᮏヨ㦂ἲࡢ᭷⏝ᛶ࡜㐺⏝㝈⏺
ᮏヨ㦂ἲࡣࠊື≀ࢆ౑⏝ࡋ࡚࠾ࡽࡎࠊື≀⚟♴㠃࠿ࡽ௦᭰ἲ࡜ࡋ࡚ጇᙜ࡛࠶ࡿࠋᮏヨ㦂ἲࡢࢩࢫࢸ࣒ࡣၟᴗⓗ࡟ࡣ Corrositex®࡜ࡋ࡚㈍኎ࡉࢀ࡚࠾ࡾࠊ⭷ࣂࣜ࢔ヨᩱࡢᛶ⬟ࡢᆒ୍ᛶࢆ
ಖࡘࡇ࡜ࡣẚ㍑ⓗᐜ᫆࡜ᛮࢃࢀࠊ≉ู࡞ᡭᢏࡶᚲせ࡞࠸࡜ᛮࢃࢀࡿࡇ࡜࠿ࡽࠊᢏ⾡⛣㌿ᛶࡣ㧗࠸࡜ุ᩿࡛ࡁࡿࠋࡲࡓࠊ⩦⇍ᗘ☜ㄆ≀㉁ࢆ⏝࠸࡚ᑓ㛛ᢏ⾡ࡢ⩦⇍࡟ࡘ࠸࡚☜ㄆࡍࡿࡇ࡜ࡀ࡛ࡁࠊከࡃࡢ⩦⇍ᗘ☜ㄆ≀㉁ࢆ୍ᐃࡢ᮲௳࡛ホ౯ࡍࡿࡇ࡜࡟ࡼࡾࠊCDS ࡢኚ໬ࡢ⛬ᗘ࡟ࡼࡿ⭉㣗ᛶศ㢮ࡢ⢭ᗘࢆ㧗ࡵࡿࡇ࡜ࡀ
࡛ࡁࡿ࡜᥎ࡉࢀࡿࠋ

Corrositex®ἲࡢணᛶ࡟ࡘ࠸࡚ࠊICCVAM ࡟ࡼࡿ㞟ィ⤖ᯝ࡜ Botham ࠾ࡼࡧ Fentem ࡢㄽᩥ࡟グ㍕ࡉ

ࢀࡓឤᗘࠊṇ☜ᗘࠊ≉␗ᗘ࡛♧ࡍ࡜ࠊṇ☜ᗘ 73-80㸣ࠊឤᗘ 71-89㸣ࠊ≉␗ᗘ 65-76㸣࡛࠶ࡗࡓ 3,5,7)ࠋࡓ
ࡔࡋࠊࡇࢀࡽࡢ⤖ᯝࡣヨ㦂㛫࡛ᕪࡀ኱ࡁࡃࠊ⓶⭵⭉㣗ᛶヨ㦂㈨ᩱ⦅⧩ጤဨ఍࡛ࡣࠊホ౯≀㉁㑅ᢥࡢᐈほᛶ࡞࡝ࡶ⪃៖ࡋ࡚ࠊFentem ࡢ⤖ᯝࢆ᥇⏝ࡍࡿࡇ࡜ࡀጇᙜ࡜ุ᩿ࡋ࡚࠸ࡿࠋࡇࡢ Fentem ࡢணᛶࡢ⤖ᯝࢆ⾲ 1 ࡟♧ࡋࡓ㸦ṇ☜ᗘ 73㸣ࠊឤᗘ 71㸣ࠊ≉␗ᗘ 76㸣࡛࠶ࡾࠊഇ㝜ᛶ⋡ 29㸣ࠊഇ㝧ᛶ⋡ 24%࡜࡞ࡿ㸧ࠋࡋࡓࡀࡗ࡚ࠊᮏヨ㦂ἲࡣഇ㝜ᛶ࡜࡞ࡿሙྜࡀከ࠸࡜ุ᩿ࡉࢀࡿࠋ⓶⭵⭉㣗ᛶヨ㦂㈨ᩱ⦅⧩ጤဨ఍࡟ࡼࡾసᡂࡉࢀࡓホ౯ሗ࿌᭩ 2)࡟ࡼࢀࡤࠊCorrositex®ἲࡢࡶࡗ࡜ࡶ኱ࡁ࡞ၥ㢟Ⅼࡣࠊࣂࣜࢹ࣮ࢩࣙࣥ◊✲࡛㑅ᢥࡉࢀࡓ≀㉁ࡢ୰࡛ࠊヨ㦂࡟㐺⏝࡛ࡁࡿ≀㉁ࡀ㝈ᐃࡉࢀ࡚࠸ࡿࡇ࡜࡛࠶ࡿ㸦㐺ྜᛶヨ㦂࡟ࡼࡾ Botham ࡛ࡣ 70-76%ࠊFentem ࡛ࡣ 63%ࡋ࠿ヨ㦂ࢆᐇ᪋࡛ࡁ࡞࠿ࡗࡓ㸧ࠋCorrositex®ἲࡣཎ⌮ⓗ࡟ CDS ࡢⰍࡲࡓࡣ௚ࡢ≉ᛶࡢኚ໬ࢆ⏕ࡌ࡞࠸≀㉁࡟ࡣ㐺⏝࡛ࡁ࡞࠸ࠋ౛࠼ࡤࠊpHࡀ 4.5~8.5 ࡢ⠊ᅖࡢỈ⁐ᛶ≀㉁ࡣࠊከࡃࡢሙྜホ౯࡛ࡁ࡞࠸ࠋࡇࡢࡓࡵࠊ⭷ࣂࣜ࢔ࢆ⏝࠸ࡓヨ㦂ࢆ㛤ጞࡍࡿ๓࡟ࠊ㐺ྜᛶヨ㦂࡜ࡋ࡚ࠊ⿕㦂≀㉁ࡀ CDS ࡟ࡼࡗ᳨࡚ฟྍ⬟࠿࡝࠺࠿ࢆ☜ㄆࡋ࡚࠾ࡃᚲせࡀ࠶ࡿࠋࡇࡢࡼ࠺࡟㐺⏝࡛ࡁࡿ≀㉁࡟ࡘ࠸࡚ὀពࡍࡿᚲせࡀ࠶ࡿࡶࡢࡢࠊᮏヨ㦂ἲࡣᅛయ㸦Ỉ⁐ᛶ/㠀Ỉ⁐ᛶ㸧ࠊᾮయ㸦Ỉ⁐ᾮ/㠀Ỉ⁐ᾮ㸧࠾ࡼࡧங๣࡟㐺⏝࡛ࡁࡿࠋ⾲ 1. Corrositex®ࡢணᛶFentem et al.㸦1998㸧5)

ṇ☜ᗘ

73%

ឤᗘ71%

≉␗ᗘ76%

ホ౯≀㉁ᩘ

38/60

44

OECD ࡢ in vitro ⭉㣗ᛶヨ㦂࢞࢖ࢻࣛ࢖ࣥ࡜ࡋ࡚ࠊ௚࡟ࠕTG431 ࣄࢺ⾲⓶ࣔࢹࣝヨ㦂ࠖ࠾ࡼࡧࠕTG430⤒⓶㟁Ẽ᢬ᢠヨ㦂㸦TER㸧ࠖࡀ࠶ࡾࠊICCVAM ࡛ࡣᮏヨ㦂ἲ࡜ࡑࢀࡽࡢヨ㦂ἲ (EpiSkinτ, EpiDermτ࠾ࡼࡧ TER)࡟ࡘ࠸࡚≉␗ᛶࠊឤᗘࠊṇ☜ᛶ➼ࡀẚ㍑ࡉࢀ࡚࠸ࡿ 2,7,8)ࠋヨ㦂≀㉁ࡢᩘ㔞ࡸ✀㢮ࡀ␗࡞ࡗ࡚࠸ࡿࡓࡵࠊ⤖ᯝࡢุᐃࡔࡅࢆࡶࡗ࡚ࠊ༢⣧࡟ᙜヱヨ㦂ἲࡢඃ㉺ᛶࢆホ౯ࡍࡿࡇ࡜ࡣᅔ㞴࡛࠶ࡿࡶࡢࡢࠊᮏヨ㦂ἲࡣࠊ௚ࡢヨ㦂ἲ࡜ẚ࡭࡚ឤᗘࡀప࠸࡜ุ᩿ࡉࢀࡿ㸦EpiSkin™࡜ TER ࡢឤᗘࡣࡑࢀࡒࢀ 98.5%࡜
94%㸧2)ࠋ
௨ୖࡢⅬ࠿ࡽࠊCorrositex®ἲࡣ୍㒊ࡢ⿕㦂≀㉁࡜ΰྜ≀࡟࠾࠸࡚⭉㣗ᛶࡢ᭷↓ࢆホ౯࡛ࡁࡿ࡜⪃࠼ࡽࢀࡿࡶࡢࡢࠊࡑࡢ㐺⏝⠊ᅖࡣ⊃ࡃࠊࡲࡓࠊ㐺⏝ྍ⬟≀㉁ࡢ⠊ᅖࡀ୙᫂☜࡛࠶ࡿࠋࡋࡓࡀࡗ࡚ࠊᮏヨ㦂ἲࡣከᩘࡢ≀㉁ࢆࢫࢡ࣮ࣜࢽࣥࢢホ౯ࡍࡿ┠ⓗ࡟ࡣ㐺ࡋ࡚࠸࡞࠸࡜⪃࠼ࡽࢀࡿࠋࡲࡓࠊ௚ࡢヨ㦂ἲ࡜ẚ࡭࡚ഇ㝜ᛶࡀከ࠸ࡇ࡜࡟␃ពࡍࡿᚲせࡀ࠶ࡿࠋ࡞࠾ࠊin vitro ⭷ࣂࣜ࢔ヨ㦂ἲ࡟࠾ࡅࡿ᳨ฟ᪉ἲ࡜ࡋ࡚ࡣࠊⰍࡢኚ໬ࡢ௚࡟ࠊ໬Ꮫ཯ᛂࠊ㟁Ẽ໬Ꮫ཯ᛂࡶཎ⌮ⓗ࡟ࡣ㑅ᢥྍ⬟࡛࠶ࡾ 1)ࠊ㐺⏝⠊ᅖࢆᣑᙇ࡛ࡁࡿྍ⬟ᛶࡣ࠶ࡿࡀࠊࡑࡢࡼ࠺࡞᳨ฟ᪉ἲࢆྵࡵࡓࣂࣜࢹ࣮ࢩࣙࣥ◊✲ࡣࡇࢀࡲ࡛࡟⾜ࢃࢀ࡚࠸࡞࠸࡜ᛮࢃࢀࡿࠋ㸲㸬┠ⓗ࡜ࡍࡿ≀㉁ཪࡣ〇ရࡢẘᛶࢆホ౯ࡍࡿヨ㦂ἲ࡜ࡋ࡚ࡢࠊ♫఍ⓗཷࡅධࢀᛶཬࡧ⾜ᨻୖࡢ฼⏝ࡢྍ⬟ᛶ ♫఍ⓗཷࡅධࢀᛶ㸸ᮏヨ㦂ἲࡣࠊ≉ู࡞ᡭᢏࡀᚲせ࡛࡞࠸ヨ㦂ἲ࡛࠶ࡾࠊᮏヨ㦂ἲࡢࢩࢫࢸ࣒ࡣᕷ㈍ࡉࢀ࡚࠸ࡿࠋࡲࡓࠊ
⏕ࡁࡓື≀ࢆ⏝࠸࡞࠸࡜࠸࠺Ⅼ࡛ࠊ3Rs ࡢ⢭⚄࡟ྜ⮴ࡋ࡚࠾ࡾࠊ♫఍ⓗཷࡅධࢀᛶࡣ㧗࠸ࠋ ⾜ᨻୖࡢ฼⏝ᛶ㸸Corrositex®ἲࡣ㐺⏝ྍ⬟≀㉁࡟ CDS ౑⏝ୖࡢไ⣙ࡀ࠶ࡾࠊഇ㝜ᛶࡀከ࠸ࡇ࡜࠿ࡽࠊከᵝ࡞≀㉁ࢆࢫࢡ
࣮ࣜࢽࣥࢢホ౯ࡍࡿ┠ⓗ࡟ࡣ㐺ࡋ࡚࠸࡞࠸࡜⪃࠼ࡽࢀࡿࠋࡓࡔࡋࠊᮏヨ㦂ἲ࡟ࡼࡗ࡚㝧ᛶ࡜ุᐃࡉࢀࡓࡶࡢࢆ㝧ᛶ≀㉁࡜ࡋ࡚㆑ูࡍࡿࡇ࡜࡟㝈ᐃࡍࢀࡤࠊ฼⏝ྍ⬟࡜ุ᩿ࡍࡿࠋCorrositex®௨እࡢ in vitro ⭷ࣂࣜ࢔ヨ㦂ἲ࡟ࡘ࠸࡚ࡣ▱ࡽࢀ࡚࠸࡞࠸ࡀࠊ㛤Ⓨࡉࢀࡓሙྜ࡟ࡣ༑ศ࡞ࣂࣜࢹ࣮ࢩࣙࣥ⤖ᯝ࡟ᇶ࡙࠸࡚ࠊࡑࡢ⾜ᨻⓗ฼⏝ᛶࡢホ౯ࢆ⾜࠺࡭ࡁ࡛࠶ࡿࠋཧ⪃ᩥ⊩1)

OECD (2015), Guidelines for the Testing of Chemicals, Test No. 435: In Vitro membrane barrier test method

2)

JaCVAM ⓶⭵⭉㣗ᛶ㈨ᩱ⦅⧩ጤဨ఍㸸⓶⭵⭉㣗ᛶヨ㦂ホ౯ሗ࿌᭩ In vitro ⭷ࣂࣜ࢔ヨ㦂ࢆ⏝࠸ࡓ⓶⭵⭉㣗ᛶヨ㦂௦᭰ἲࡢホ౯ሗ࿌᭩㸦2017 ᖺ 2 ᭶ 24 ᪥㸧㸬3)

Botham, P.A. et al. (1995), A prevalidation study on in vitro skin corrosivity testing: The report andrecommendations of ECVAM workshop 6. ATLA, 23, 219-2554)

Barratt, M.D. et al. (1998), The ECVAM international validation study on in vitro tests for skin corrosivity. 1.
Selection and distribution of the test chemicals. Toxicology in Vitro, 12, 471-482555)

Fentem, J.H. et al. (1998), The ECVAM international validation study on in vitro tests for skin corrosivity. 2.
Results and evaluation by the management team. Toxicology in Vitro, 12, 483-5246)

ECVAM. (2001), Statement on the application of the CORROSITEX® Assay for Skin Corrosivity Testing.
15th Meeting of ECVAM Scientific Advisory Committee (ESAC), Ispra, Italy. ATLA 29, 96-97.

7)ICCVAM (1999), NIH Publication No.99-4495. Corrositex: An in vitro test method for assessing dermal
corrosivity potential of chemicals.

8)

ICCVAM (2002), NIH Publication No.02-4502. ICCVAM evaluation of EPISKIN, and EpiDerm (EPI-200)
and rat skin transcutaneous electrical resistance (TER) assay: in vitro test method for assessing dermal
corrosivity potential of chemicals.

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