Gene Engineering���� ���������
DNA is synthesized as you like, and inserted to the vector.
This service is more accurate and cheaper than PCR-amplifying thee targeted DNA fraction from cＤＮＡ library.

P dP��d���
LL�����
�ength of GenePrice （Incl. Optimization of codon）

0-3kkb

3-10kb¥110/mmer～

Please ask us* The price may be changeable according to the length and sequence (GCC% repeat sequence,C%,sequence etc).
etc)* Free if BEX standard vector ｐUC57 is used for cloning.

�������� ����Our staff contact you after the order.

�������� P��d���・ About 4μg of pUC57 vector with the targeted DNA inserted into （freeze-dried）（・ Sequence Data�����������
・Our staff may contact you depending on the difficulty of synthesis.
・The synthesis of the gene with toxicity isn’t acceptable.

���� �� ��d��Please send your requests to us.

BEX CO.,�TD.

http://www.bexnet.co.jp

Please email orr send the order formfdownloaded fromf
BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

18Gene Engineering�����on�n�
Insert of the targeted gene from a vector into another vector by reestriction enzyme or PCR.
Subcloning 2kb of the targeted gene and single strand sequencing forf the check as the standard operation.

�ro���� ��ne������

Subcloning （Restriction Enzyme Method *1）¥80,000/ Sample> 2 weeks

Subcloning （PCR *2）¥100,000/ Sample> 3 weeks*1 Applied only to the same restriction enzyme site before and after subcloning (incl. enzyme to cut the same sequence).
*2 A new site for the restriction enzyme is added to the end of the targeted gene sequence by PCR. Afterwards, the fraction
is inserted into another vector.

�e���er� �ro����・Prepared Plasmid DNA（ > 10μg）
・Operation Report・Spectra Data file�n�or����on・If sequencing more than 2kb gene, additional fee for the sequence analysis
ashould be payable.
・The actual fee is payable in the unavoidable termination after the discussion with the customers.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

F�o� �o �r�er
Please email orr send the order form downloaded from BEX web page withthe text file of the sequence data of the targeted gene.
E-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Plasmid DNA with the targeted gene inserted into: 2μg( > 100ng/μl)
・Vector to be inserted into: 2μg ( > 100ng/μl)
Frozen・Order form
II. Please send the sample and order form to us.
Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+BEX CO.,LTD.

19

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

Gene Engineeringc��� ��on�n�
PCR of the targeted gene with 3 kinds of polymerase at most.
t method, or blunt end method.
Insert of the targeted gene by TA cloning, restriction-enzyme site tag�ro��c� ��ne��c��� ��on�n�
PrriceDeliverycDNA Cloning¥190,0000/ Sample> 3 weeks

Additional fee for Clone Length¥30,0000/kb> 3 Business Days

* The sequence is checked by double strand sequencing after cloning.
* cDNA-cloning 1kb of the targeted gene as the standard operation.

����onPrrice

DeliverycDNA Preparation from cells・tissues¥500,000> 3 Business Days

cDNA Preparation from Total RNA¥100,000> 2 Business Days�e���er� ���e・Prepared Plasmid DNA （ > 10μg）
・OperationpReportp・Spectra Data File�n�or����on
・The actual fee is payable in the unavoidable termination after the discussion with the customers.
・The delivery date depends on the operation.
The freight of the sample to BEX is payablepayable.
・TheF�o� �o �r�er
Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

II. Please send the sample and order form to us.

Frozen・cDNA（ > 1μgg )・Vector 2μg ( > 100ng/μl) Frozen
・In the case off the option
Cell （About 5××106 cells） / Tissue （About 10mg） / Total RNA（ > 10μg）
・Order form
FrozenAddress: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+BEX CO.,LTD.

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jpphone +81-3-5375-1071

fax +81-3-5375-5636

20

Gene Engineering���e-��re�� ������on
Mutation into the specific site in the targeted gene.
Amino Acid Substitution・Base Insertion・Tag Insertion・Restriction Enzyme Site Insertion・Deletion Mutant・Mutation of Multiple Site, etc.

�ro���� ��ne��

Site-Direct Mutation

PrriceDelivery¥90,0000/ site

> 3 weeks* If a mutation site is close to another (within 15 bp), Two-site mutation is accepted as one-site mutation for the price.
* Site-direct mutate 2kb of the targeted gene and single strand sequencinng for the check as the standard operation.

�e���er� �ro����・Prepared Plasmid DNA（ > 10μg）
・Operation Report・Spectra Data File�n�or����on・If sequencing more than 2kb gene, additional fee for the sequence analysis
ashould be payable.
・The actual fee is payable in the unavoidable termination after the discussion with the customers.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

F�o� �o �r�er
Please email orr send the order form downloaded from BEX web page withthe text file of the sequence data of the targeted gene and detail of themutation site.
FAX : +81-3-5375-5636
E-mail : info@bbexnet.co.jp

I. Please send your requests to us.

・Plasmid DNA with the targeted gene inserted into: 2μg(> 100ng/μl)
O d formf・Order
FFrozenII. Please send the sample and order form to us.

BEX CO.,LTD.

21

http://www.bexnet.co.jp

Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636Gene Engineering������d �re��r���on
The best grade for transfection・ in vitro transcription, etc.

�rod��� ��ne��
�ieldPrice

100μg¥9,0001mg¥20,0002mg¥38,000

3mg¥54,000

4mg¥72,000

5mg¥90,000

6mg¥108,000

7mg¥126,000

8mg¥144,000

9mg¥162,000

10mg¥180,000�e���er� ���e
Our Staff contacts you after the order.

�e���er� �rod���・Prepared Plasmid DNA
・Electrophoresis Data�n�or����on
・The price of the low-copy plasmid is changeable.
・The actual fee is payable in the unavoidable termination after the discussion with the customers.
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

F�F�o�
�o OrderO dPlease email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Plasmid （ > 1μg ） / Glycerol Stock （ about 1ml） Frozen
・Order form
II. Please send the sample and order form to us.

BEX CO.,LTD.

http://www.bexnet.co.jpAddress: 〒17333-0004
0004 4F 61
61-15
15 22-Chome
Chome Itabashi-ku
Itabashi ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

22