     

mRNA  A 63!"ǠĶĚǸƦŕĲĥǸ·ƩůèµřǓäêäêǧ ŬÍƀêǧ
060-0810 µřǓĺÿüµ¶µ 10 ĻƲ 8 }ŸµřǓäêŪêǘ 5 ÄǵYuya Suzuki, Tomohiro Arae and Yukako ChibaPoly(A)-dependent Post-transcriptional Regulations
Keywords: deadenylation, mRNA decay, Poly(A), Post-transcriptional Regulation
Graduate School of Life Science, Hokkaido University
Sapporo, Hokkaido, 060-0810 Japan
DOI: 10.24480/bsj-review.8b8.00118

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ƚƷ²Ũ&®Ēħ587mRNA ' 5’ĸƆ&FoUe

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ŦŇǎ;Á7 (Tarun & Sachs, 1996)&ūŦŇǎ%46ǸƚƷƑƓď%|Ą mRNAǬthR{l"ƛ¥* mRNA & 5’ĸƆ%tGu{X82$6ǸƚƷ²Ũ

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ǚŒ;ŭit A Ǣǣ&ƖƘŶ$ÇïĠŗ&žƅ%46Ǹit A Ǣǣ" mRNA ~&thR{l&óĄ%Ŏ&ŹǥǚŒ%ſ8 (Beilharz & Preiss, 2007; Lackner et al., 2007)&"'Ǹǣit A Ǣ;Ħ mRNA 'ƚƷ²ŨǷÈ|į! PAL-seq "thR{lewc=@tyH%47Ƶļ

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DVmEBu!'ƝŴŬ¬ĵ%7ƞƝĵ.!&Əƞ!it A Ǣǣ"ƚƷ²Ũ%Ŏ&Źǥ
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7it A Ǣ

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it A Ǣ©Ƶ;

7 (Yamashita et al., 2005)

ƭ

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it A ǢmRNA &ëï´%ǜƳ!7";ſ5'Ǹit A Ǣǣ"¸Ŝĵ&Źǥǥ;ƴ¨"!

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4*ŃŤ;ŭƵļ!'Ǹit A Ǣǣ"¸Ŝĵ

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et al., 2014; Kappel et al., 2015)&Ǖſ8 (Beilharz & Preiss, 2007; Subtelny

ðǶƌ&Ǖ%471&!7ÃƟĘ17mRNA &it A Ǣǣ"¸Ŝĵ&ǥ%

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ņƻ7

;798CCR4ŃŤ%7 CCR4 'Lw@\ZOZ"@]!ƴ(AtCCR4) 'ǚŒ Ccr4p &gnwH"
@\ZOZ! 7 &Ǘè

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5$7c=kt{;ĊğǸ!1 AtCCR4a4* AtCCR4b

ǚŒ Ccr4p "Ĵ1ǷŹÇĘ;ſatccr4a/4b ǜáŲŀ;ŭǊǗêŶƵļAtCCR4a/4b

Wyeyƾ%ǥ:

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6Ǹ5%Ǹ>kw{NÆğǚƎ;J{Y7 Granule-bound starch synthase 1 (GBSS1) mRNA &it A Ǣǣ&"5ǸAtCCR4a/4b %4&Ǟ&®Ē%ǜƳ!7"

:

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6ǸƬ

5ǸAtCCR4a/4b 'AtDCP1/2ǸAtXRN4 "& mRNA ©ƵǚƎ" P-body %8 (Suzuki et al., 2015)OsCCR4a7ǚŒ Ccr4p &gnwH

4* OsCCR4b '@]%

Źŭ7"ſ!6Ǹ851. P-body %øÕ75%ǸOsCCR4a/4b ' in vitro !it A Ǣ&©Ƶ
ŘĘ;ſ (Chou et al., 2017)EEP c=kt{%É.87Ơ>W[u´ǚƎ'ǸCCR4Ǹ
NocturninǸPDE12ǸAngel & 4 &KdHu{e%©ǳ8ǸAtCCR4a/4bǸOsCCR4a/4b '"1% CCR4 Hu{e%ú7 (Dupressoir et al., 2001; Chou et al., 2017)AtHesperin (AtHESP)'Î

ǳ& Nocturnin &Lw@\ZOZ%$©ƵŘĘ

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;7:8CAF1ŃŤ%7 CAF1 'Lw@\ZOZǸ@]ǸXAEsL!ƴ& CAF1 (CaCAF1) 'ų¼Ƨ&ĝľ%4

Ŵũ

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4

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"!ŃŤ%ĈųñĤģĘ;15Ǹ&ǒ¯Ŵũŀ!1 PR1
Ŵũ

4* PR2 &Ǘè.ǸAtCAF1a/b ' in vitro !it A Ǣ&©ƵŘĘ;ſǸAtCAF1a/b

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MoNnyǛƺõĘǗè!7 Vegetative storage protein 1 (VSP1)

2NXvNǥǏǗè!7 Chitinase B (CHIB)Ǹ Lipoxygenase2&ťŲŶ$ŉŶ mRNA "

(LOX2)Ǹ5% AtCAF1b

Phosphatidyl -inositol 4-kinase (PI4Kg3)

al., 2009; Walley et al., 2010)85&";

4* PR6 &Çï8 (Liang et

5ǸCAF1 'ťï& mRNA &it A Ǣǣ&ƼƉǸų¼ĝľ$#&ŬŤŶNXvNĖƈ%ǥ:

7 CAF1 &gnwH!7 OsCAF1b %ǥ7"ƛ'Ǹ&Ŵũ

Ėƈ!ƺõ87"Ǹ5% AtXRN4 "&£øÕƵļ

587@]%

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5 P-body %øÕ7"ſ

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7(Chiba et al., 2004; Reverdatto et al., 2004).ǸAtPARN &Ş¨ƄŢáŲŀ'Ǹ¤Ŭ& ABA Ǟ
à±70%âŬ& ABA %ô2005)ƤËŚ"%ǸAtPARN

ǷĝÂĘ"$7Ưũ×;ſ (Nishimura et al.,kXJyYt>%øÕ7Ơ>W[u´ǚƎ!7"ſ8Ǹit A itms{Q"¹ƼkXJyYt> mRNA &it A Ǣǣ&®ĒŊŇı5"$ (Hirayama et al., 2013)&"t>$#&ƏƞöÑî%<8..



5ǸŁ2Əƞǃ!$ǸkXJyY

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źŁŬŤ%ăé8Ƴ$Ơ>W[u´ǚƎ&ǸCCR4
NOT ƱÆ"ûä$Sy`GǃƱÆ;Ċğ

4* CAF1 ' CCR4-

7.Ǹ85&Ơ>W[u´ǚƎ'ŉŶ mRNA ;ƹƿ70%ĔƳ$ RNA ƓÆYm@y;Ħ$&0ŉŶ mRNA
&ƹƿ%' CCR4-NOT ƱÆ"ǸmRNA & 3’UTR %éÕ7ÞÛǙ«2ŌŇǎ;ƹƿ
7 RNA ƓÆSy`Gǃ$#&ƼƉÒè"&ŹŭĔƳ!7"ǸǚŒ2Î

ƃ!ſ8!' CCR4-NOT ƱÆ"ŉŶ mRNA ƹƿŊŇ&ņƳǸ

ǳ&Ž
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<798CCR4-NOT .
CCR4-NOT ƱÆ'ǸNOT1 ;ǅÝSy`Gǃ"
NOT10/11 $#CCR4/CAF1ǸNOT2/3/5ǸNOT4ǸNOT9Ǹ

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7 (Collart, 2003; Shirai et al., 2014)ǚŒ& CCR4-NOT ƱÆ' Ccr4p/Caf1p %47Ơ>W[u´ÀĖ&®Ē!$Ǹ&Kdq[UX%47Ǉ¦2Sy`Gǃ©Ƶ&®Ē$#ǸǗèŴũ&ň$őǪ&®Ē%ǥ:
7ǚŒ CCR4-NOT ƱÆ&Kdq[UX!7 Not4p ' E3 qbFTytE{Q!6ǸmRNA ~!ō7" (ƚƷ>vNX) %46ƺõ87Sy`GƚƷ&thR{lǃ©Ƶ,&ǥſÏ8

7 (Mulder et al., 2007; Dimitrova et al., 2009; Inada & Makino,2014)ƚƷ>vNX' mRNA

5&thR{l&ƵǬ;Ć

Ǆ&"Ćǟ"$

6ǸNot4p %47ƚƷ

ǋ!Ƒì¢$Sy`Gǃ&©Ƶǐ2Ǹ&ǡ×"$

7 mRNA &©ƵǄ7.ǸNot2/3/5 ' RNA itms{Q&Kdq[UX!7

Mediator of RNA polymerase II transcription subunit 17 (Med17p) "ŹŭǸǇ¦®Ē%ǥ7 (Lee et al., 1998)
XdDR#WJf*o3 5 < I t m n A H  %]C) 1=60`#    'j$
1=60`SA1=60`nAH)o-35<Itm)   %iF   ,Ug) SA49.=,Q[lF"iF   $Ug%

SA1=60`"!$LqkG nAH MpVK) #(@+*)%    ŃŤ% !&,)

1 CCR4-NOT ƱÆ&J>Kdq[UX'é8" OsNOT1
2017)

*+(#'

OsCAF1 ;
$ŹŭƱÆ;Ċğ7"

6Ǹ@]! OsCCR4ſ8 (Chou et al.,5ǸŃŤ& CCR4-NOT ƱÆ&¢ò'ķƵı!ǸÅKdq[UX&gnw

H%ǥ7Žƃ1.÷$ǚŒ Not2p &Lw@\ZOZ%

7gnwH!7

AtNOT2a/b 'ǸDicer-like 1 (DCL1) 2 SERRATE (SE) $#ǸmiRNA &ğţ%ǥ:7Sy`Gǃ"Ł!Źŭ7 (Wang et al., 2013). AtNOT2a/b &ŊƟŋĬáŲŀ'ƣŏ&Ưũ×;ſǸewn{S{ǱØ,& T-DNA &Ĩ¡¿* RNAi %4

AtNOT2a/b &Ŵũ7ŃŤ!1Ʃ&ĊĞŲþ2ƥğ&Ǒą;ſǸmiRNA ;É/ã&Ǘè&Ǉ¦®873% CCR4-NOT ƱÆ'ŃŤ%$ƱÆ"

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