ホ౯఍㆟ሗ࿌᭩෌ᵓ⠏ࣄࢺゅ⭷ᵝୖ⓶ࣔࢹࣝἲ㸦RhCE ἲ㸧
LabCyte CORNEA-MODEL24 ║่⃭ᛶヨ㦂
㸦LabCyte CORNEA-MODEL24 EIT㸧JaCVAM ホ౯఍㆟

ᖹᡂ 31 ᖺ㸦2 019 ᖺ㸧2 ᭶ 19 ᪥

11

JaCVAM ホ౯఍㆟኱ 㔝 Ὀ 㞝 㸦බ┈㈈ᅋἲே ᮌཎグᛕᶓ὾⏕࿨⛉Ꮫ᣺⯆㈈ᅋ㸧㸸ᗙ㛗஬༑ᔒⰋ᫂㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ㸧▼ ஭ 㞝 ஧㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ Ᏻ඲ᛶ⏕≀ヨ㦂◊✲ࢭࣥࢱ࣮㸧✄ ⱝ 㑥 ᩥ㸦᪥ᮏ໬Ꮫᕤᴗ༠఍㸧஭ ୖ ᬛ ᙲ 㸦᪥ᮏච␿ẘᛶᏛ఍㸧௒ ஭ ᩍ Ᏻ 㸦᪥ᮏື≀ᐇ㦂௦᭰ἲᏛ఍㸧ᒾ℩⿱⨾Ꮚ 㸦᪥ᮏ〇⸆ᕤᴗ༠఍㸧ஂ ಖ ᩥ ᏹ㸦⊂❧⾜ᨻἲே ་⸆ရ་⒪ᶵჾ⥲ྜᶵᵓ㸧ᮡᒣ┿⌮Ꮚ㸦᪥ᮏ໬⢝ရᕤᴗ㐃ྜ఍㸧୰ᮧࡿࡾࡇ㸦⊂❧⾜ᨻἲே 〇ရホ౯ᢏ⾡ᇶ┙ᶵᵓ㸧す ᕝ ⛅ ె㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ Ᏻ඲ᛶ⏕≀ヨ㦂◊✲ࢭࣥࢱ࣮῭⏕఍Ᏹ㒔ᐑ⑓㝔㸧す ᮧ ḟ ᖹ㸦⊂❧⾜ᨻἲே ་⸆ရ་⒪ᶵჾ⥲ྜᶵᵓ㸧἟ ⃝  ⪽㸦᪥ᮏẘᛶᏛ఍㸧ᖹ ᯘ ᐜ Ꮚ㸦ᅜ❧་⸆ရ㣗ရ⾨⏕◊✲ᡤ Ᏻ඲ᛶ⏕≀ヨ㦂◊✲ࢭࣥࢱ࣮㸧 ቑ ᮧ ೺ ୍㸦᪥ᮏ⎔ቃኚ␗ཎᏛ఍㸧ᶓ 㛵 ༤ 㞝㸦᪥ᮏ⓶⭵ච␿࢔ࣞࣝࢠ࣮Ꮫ఍㸧௵ᮇ䠖ᖹᡂ 30 ᖺ 4 ᭶ 1 ᪥䡚ᖹᡂ 32 ᖺ 3 ᭶ 31 ᪥22෌ᵓ⠏ࣄࢺゅ⭷ᵝୖ⓶ࣔࢹࣝἲ㸦Reconstructed human Cornea-like Epithelium Test Method: RhCE ἲ㸧ࡣࠊ࢘ࢧࢠࢆ⏝࠸ࡓ Draize ║่⃭ᛶヨ㦂ࡢ௦᭰ヨ㦂ἲ࡜ࡋ࡚ࠊ⿕㦂≀㉁ࡢࣄࢺゅ⭷ᵝୖ⓶ࣔࢹࣝ⤌⧊࡟ᑐࡍࡿ⣽⬊ẘᛶࢆᣦᶆ࡟⏝࠸ࠊࡑࡢ≀㉁ࡢ║่⃭ᛶࢆホ౯ࡍࡿヨ㦂ἲ࡛࠶ࡿࠋOECD TG492 ࡟ࡣࠊ࣎ࢺ࣒
࢔ࢵࣉ᪉ᘧ࡛ United Nations Globally Harmonized System of Classification and Labelling of Chemicals㸦UNGHS㸧༊ศእ≀㉁ࢆ᳨ฟ࡛ࡁࡿ EpiOcularTM ║่⃭ᛶヨ㦂㸦EpiOcularTM Eye Irritation Test: EpiOcularTM EIT㸧࠾ࡼࡧ SkinEthicTM ࣄࢺゅ⭷ୖ⓶ࣔࢹࣝ║่⃭ᛶヨ㦂㸦SkinEthicTM Human Corneal Epithelium Eye IrritationTest: SkinEthicTM HCE EIT㸧ࡀ ESAC㸦EURL ECVAM Scientific Advisory Committee㸧ࡢ➨୕⪅ホ౯ࢆ⤒࡚RhCE ἲࡢ᳨ド῭ࡳᶆ‽ヨ㦂ἲ㸦Validated Reference Method: VRM㸧࡜ࡋ࡚཰㍕ࡉࢀ࡚࠸ࡿ 1)ࠋࡲࡓࠊOECD
ࡣ᪂つ RhCE ⤌⧊ࢆ⏝࠸ࡓヨ㦂ἲ࡟ᑐࡋ࡚ࡑࡢᵓ㐀࠾ࡼࡧᶵᗎࡀ VRM ࡜ྠ➼࡛༑ศ࡞ಙ㢗ᛶࢆഛ࠼࡚࠸ࡿࡇ࡜ࢆホ౯ࡍࡿᛶ⬟ᶆ‽ࢆᐃࡵ࡚࠸ࡿ 2) ࠋLabCyte CORNEA-MODEL24 ࢆ⏝࠸ࡓ║่⃭ᛶヨ㦂
㸦LabCyte CORNEA-MODEL24 Eye Irritation Test: LabCyte CORNEA-MODEL24 EIT㸧ࡣ㢮ఝヨ㦂ἲ࡜ࡋ࡚ࠊOECD ࡢᛶ⬟ᶆ‽࡛ᣦᐃࡉࢀ࡚࠸ࡿ 30 ࡢཧ↷≀㉁ࢆ⏝࠸ࡓ 3 ᪋タ࡛ࡢ㏣㉮ⓗࣂࣜࢹ࣮ࢩࣙࣥ◊✲ 3)
࠾ࡼࡧ JaCVAM ࡢ➨୕⪅ホ౯ 4)ࢆ⤒࡚ࠊ2018 ᖺ࡟ TG492 ࡟཰㍕ࡉࢀࡓ 5)ࠋJaCVAM ホ౯఍㆟ࡣࠊ║่⃭ᛶヨ㦂㈨ᩱ⦅⧩ጤဨ఍࡟ࡼࡾసᡂࡉࢀࡓࠕᨵゞ OECD TG492 ෌ᵓ⠏ゅ⭷ᵝୖ⓶ࣔࢹࣝἲ LabCyteCORNEA-MODEL24 ║่⃭ᛶヨ㦂ホ౯ሗ࿌᭩ࠖ6)ࢆ⏝࠸࡚ࠊᮏヨ㦂ἲࡢጇᙜᛶ࡟ࡘ࠸᳨࡚ウࡋࡓࠋ㸯㸬ヨ㦂ἲࡢᐃ⩏ྡ⛠㸸෌ᵓ⠏ࣄࢺゅ⭷ᵝୖ⓶ࣔࢹࣝἲ㸦Reconstructed Human Cornea-like Epithelium Test Method: RhCEἲ㸧 LabCyte CORNEA-MODEL24 ║่⃭ᛶヨ㦂௦᭰ࡍࡿᑐ㇟ẘᛶヨ㦂㸸 Draize ║่⃭ᛶヨ㦂ἲヨ㦂ἲࡢᴫ␎㸸 RhCE ἲࡢࡦ࡜ࡘ࡛࠶ࡿ LabCyte CORNEA-MODEL24 EIT ࡛ࡣࠊࣄࢺゅ⭷ୖ⓶⣽⬊ࢆ㔜ᒙᇵ㣴ࡋࡓ LabCyte CORNEA-MODEL24 ࡟⿕㦂≀㉁ࡀᾮయࡢሙྜࡣ 1 ศ㛫ࠊᅛయࡢሙྜࡣ 24 ᫬㛫⿕㦂 ≀ ㉁ ࢆ ᭚ 㟢 ࡋ ࡓ ᚋ ࠊ WST-8 㸦 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)2Htetrazolium, monosodium salt㸧ࡢ㑏ඖ㔞ࢆࡶ࡜࡟ࡋࡓ⣽⬊⏕Ꮡ⋡ࢆᐃࡋࠊ║่⃭ᛶホ౯ࡢᣦᶆ࡜ࡋ࡚

⏝࠸ࡿࠋࡇࢀࡣࠊWST-8 ࡀ࣑ࢺࢥࣥࢻࣜ࢔ࡢ⬺Ỉ⣲㓝⣲ࡢᇶ㉁࡜࡞ࡿᛶ㉁ࢆ฼⏝ࡋࠊ⣽⬊ෆ࡟ྲྀࡾ㎸ࡲࢀࡓ WST-8 ࡀ⬺Ỉ⣲㓝⣲࡟ࡼࡾ㑏ඖࡉࢀࠊ⏕ᡂࡉࢀࡓ࣐࣍ࣝࢨࣥ㔞㸦㟷Ⰽ㸧ࡀ⏕Ꮡ⣽⬊ᩘ࡟ẚ౛ࡍࡿࡇ࡜࡟ᇶ࡙࠸࡚࠸ࡿࠋ⿕㦂≀㉁ࡀᾮయ࣭ᅛయ࡟㛵ࢃࡽࡎࠊᖹᆒ⣽⬊⏕Ꮡ⋡ࡀ 40㸣ࢆ㉸࠼ࡓሙྜࠊ⿕㦂≀㉁ࡣ UN GHS ศ㢮࡟࠾࠸࡚༊ศእ࡛࠶ࡿ࡜ุᐃࡍࡿࠋ࡞࠾ࠊᮏヨ㦂ἲ࡛ഇ㝧ᛶࢆᅇ㑊ࡍࡿࡓࡵ࡟ࡣࠊࡲࡓࠊUN GHS ศ㢮༊ศ 1 ࡜༊ศ 2 ࢆ㆑ูࡍࡿᚲせࡀ࠶ࡿሙྜࡣࠊ௚ࡢヨ㦂ἲ࡟ࡼࡿ㏣ຍヨ㦂ࡀᚲせ࡟࡞ࡿࠋ㸰㸬ホ౯࡟⏝࠸ࡓ㈨ᩱ࠾ࡼࡧホ౯ෆᐜࡢ⛉Ꮫⓗጇᙜᛶ║࡟␗≀ࡀධࡗࡓሙྜࠊ║ࡢ่⃭ࡣࠊ⚄⤒➼ࡢ≉ᐃࡢཷᐜయ࡟స⏝ࡍࡿሙྜࢆ㝖ࡁࠊ୍⯡࡟ゅ⭷ࡸ⤖⭷ࡢ⣽⬊യᐖ࠿ࡽጞࡲࡿࠋDraize ἲ࡟࠾ࡅࡿ║่⃭ᛶࡢ⛬ᗘࡢุᐃࡣࠊ୺࡟ゅ⭷ࡢึᮇയᐖࡢ⛬ᗘ࡟኱ࡁࡃᙳ㡪ࡉࢀࠊࡑࢀࡣゅ⭷ୖ⓶⣽⬊ࡢ⣽⬊Ṛࡢ⛬ᗘ࡜┦㛵㛵ಀ࡟࠶ࡿࠋᮏヨ㦂ἲࡣࠊࣄࢺࡢゅ⭷ୖ⓶࡟33㢮ఝࡋࡓᵓ㐀ࢆ᭷ࡍࡿ RhCE㸦LabCyte CORNEA-MODEL24㸧ࢆ⏝࠸࡚ࠊ⿕㦂≀㉁ࡢ⣽⬊ẘᛶࢆᣦᶆ࡜ࡋ
࡚║่⃭ᛶࢆホ౯ࡍࡿヨ㦂ἲ࡛࠶ࡿࠋࡇࢀࡽࡢࡇ࡜࠿ࡽࠊᮏヨ㦂ἲࡣ࢘ࢧࢠࢆ⏝࠸ࡿ║่⃭ᛶヨ㦂ࡢ௦᭰ἲ࡜ࡋ࡚⛉Ꮫⓗጇᙜᛶࡀ࠶ࡿࠋ
LabCyte CORNEA-MODEL24 EIT ࡟ࡘ࠸࡚ࡣࠊ࣮ࣜࢻࣛ࣎࡜ࡣ␗࡞ࡿ 3 ᪋タ࡟࠾࠸࡚ OECD ࡢᛶ⬟ᶆ‽࡛ᣦᐃࡉࢀ࡚࠸ࡿ 30 ࡢཧ↷≀㉁ࢆ⏝࠸ࡓ㏣㉮ⓗࣂࣜࢹ࣮ࢩࣙࣥ◊✲ࡀ⾜ࢃࢀࡓ 3)ࠋࡑࡢ⤖ᯝࠊLabCyte
CORNEA-MODEL24 EIT ࡣࠊUN GHS ༊ศእ≀㉁ண

ᛶࡢ᪋タෆ෌⌧ᛶࠊ᪋タ㛫෌⌧ᛶ࠾ࡼࡧṇ☜ᛶ࡟ࡘ࠸࡚ OECD ࡢᛶ⬟ᶆ‽ࡀᐃࡵࡓᇶ‽ࢆ‶ࡓࡋࡓࠋࡉࡽ࡟ JaCVAM ࡢ➨୕⪅ホ౯ 4)ࢆ⤒࡚ࠊUN GHS ༊ศእ≀㉁ࢆ᳨ฟࡍࡿ᪉ἲ࡜ࡋ࡚ 2018 ᖺ࡟ᨵゞ OECD TG492 ࡟㏣グࡉࢀࡓࠋJaCVAM ║่⃭ᛶヨ㦂㈨ᩱ
⦅⧩ጤဨ఍ࡣࠊࡇࢀࡽࡢ㈨ᩱࢆ⏝࠸࡚ᮏヨ㦂ἲࢆホ౯ࡋ࡚࠾ࡾࠊ⛉Ꮫⓗ࡟ጇᙜ࡛࠶ࡿ࡜⪃࠼ࡿࠋ㸱㸬ᮏヨ㦂ἲࡢ᭷⏝ᛶ࡜㐺⏝㝈⏺
RhCE ἲ࡟⏝࠸ࡿ LabCyte CORNEA-MODEL24 ࡣᕷ㈍ࡉࢀ࡚࠾ࡾࠊࡇࢀ௨እࡣ≉Ṧ࡞ᶵᮦࡸヨ⸆ࢆᚲせ࡜ࡏࡎࠊᡭᢏࡶ」㞧࡛ࡣ࡞࠸ࡇ࡜࠿ࡽᢏ⾡⛣㌿ᛶࡣ㧗࠸࡜ุ᩿࡛ࡁࡿࠋణࡋࠊධᡭࡋࡓ LabCyte
CORNEA-MODEL24 ࡀရ㉁ᇶ‽ࡢチᐜ⠊ᅖ࡟࠶ࡾࠊ࠿ࡘᐇ᪋ࡍࡿヨ㦂᪋タࡢᢏ⾡⩦ᚓࡀ࢞࢖ࢻࣛ࢖ࣥࡢ⩦⇍ᗘ☜ㄆ≀㉁࡛☜࠿ࡵࡽࢀ࡚࠸ࡿᚲせࡀ࠶ࡿࠋUN GHS ༊ศእ≀㉁ࢆ᳨ฟࡍࡿ᪉ἲ࡜ࡋ࡚ࡢಙ㢗ᛶࢆㄪ࡭ࡿࡓࡵࠊ᪋タෆ෌⌧ᛶ࠾ࡼࡧ᪋タ㛫෌⌧ᛶࢆ᳨ウࡍࡿࣂࣜࢹ࣮ࢩࣙࣥ◊✲ࡀ⾜ࢃࢀ࡚࠸ࡿࠋࣂࣜࢹ࣮ࢩࣙࣥ◊✲࡟࠾࠸࡚ࠊUN GHS ศ㢮ุᐃࡢ᪋タࡈ࡜ࡢ᪋タෆ෌⌧ᛶࡣ 93-100㸣࡛࠶ࡾࠊOECD ࡢᛶ⬟ᶆ‽ࡀᐃࡵࡓᇶ‽㸦90㸣௨ୖ㸧ࢆ‶ࡓࡋ࡚࠸ࡓ 6)ࠋ
ࡲࡓࠊ᪋タ㛫෌⌧ᛶࡣࠊ87㸣࡛ࠊOECD ࡢᛶ⬟ᶆ‽ࡀᐃࡵࡓᇶ‽㸦85㸣௨ୖ㸧ࢆ‶ࡓࡋ࡚࠸ࡓ 6)ࠋࡉࡽ
࡟ࠊࣂࣜࢹ࣮ࢩࣙࣥ◊✲࡛ᚓࡽࢀࡓࢹ࣮ࢱ࡟ࡼࡿᛶ⬟ホ౯࡛ࡣࠊឤᗘ 97.8㸣ࠊ≉␗ᗘ 68.9㸣࠾ࡼࡧṇ☜ᗘ 83.5㸣࡜࡞ࡾ OECD ࡢᛶ⬟ᶆ‽ࡀᐃࡵࡓᇶ‽㸦ឤᗘ 90%௨ୖࠊ≉␗ᗘ 60%௨ୖࠊṇ☜ᗘ 75%௨ୖ㸧ࢆ‶ࡓࡋ࡚࠸ࡓ 6)ࠋ
ణࡋࠊ⣽⬊⏕Ꮡ⋡ࡢ⟬ฟ࡟㝿ࡋࠊ⿕㦂≀㉁ࡀ WST-8 ࢆ㑏ඖࡍࡿ≀㉁ࡢሙྜࠊ࠶ࡿ࠸ࡣ࣐࣍ࣝࢨࣥ࡜ྠ
ࡌࡼ࠺࡞Ἴ㛗㸦450 nm ㏆㎶㸧࡟྾཰ࢆᣢࡘ╔Ⰽ≀㉁ࡢሙྜ࡟ࡣࠊ྾ගᗘ⿵ṇࢆ⾜࠺ᚲせࡀ࠶ࡿࠋࡑࡢᡭ
㡰࡟ࡘ࠸࡚ࡣࠊᨵゞ TG492 ࡢᮏᩥࡢㄝ᫂࠾ࡼࡧ ANNEX V ࡢࣇ࣮ࣟࢳ࣮ࣕࢺࢆཧ↷ࡍࡿᚲせࡀ࠶ࡿࠋࡲࡓࠊᮏヨ㦂ἲࢆ㐺⏝ࡍࡿ࡟ࡣࠊヨ㦂ἲࡢᛶ⬟࡜ṇ☜ᛶࡢ☜ಖࢆ⪃៖ࡋ࡚௨ୗࡢไ㝈ࡀタࡅࡽࢀࡿࠋ1) ࣂࣜࢹ࣮ࢩࣙࣥ◊✲࡟࠾࠸࡚⿕㦂≀㉁࡟ྵࡵࡽࢀ࡞࠿ࡗࡓẼయ㸦࢞ࢫ㸧࠾ࡼࡧ࢚࢔ࣟࢰࣝ≧≀㉁ࡣ㐺⏝࠿ࡽ㝖እࡉࢀࡿࠋ2) UN GHS ༊ศࡢ༊ศ 1 ≀㉁࡜༊ศ 2㸦2A/2B㸧≀㉁ࡢ㆑ู࡟ࡣ⏝࠸ࡿࡇ࡜ࡣ࡛ࡁ࡞࠸ࠋ
௨ୖࡢⅬ࠿ࡽࠊTG492 ࡟‽ᣐࡋ࡚ᐇ᪋ࡋࡓሙྜࠊ࣎ࢺ࣒࢔ࢵࣉ᪉ᘧ࡟࠾࠸࡚ UN GHS ༊ศእ≀㉁ࢆ
᳨ฟࡍࡿ᪉ἲ࡜ࡋ࡚᭷⏝࡛࠶ࡿ࡜⪃࠼ࡿࠋ

44㸲㸬┠ⓗ࡜ࡍࡿ≀㉁ཪࡣ〇ရࡢẘᛶࢆホ౯ࡍࡿヨ㦂ἲ࡜ࡋ࡚ࡢࠊ♫఍ⓗཷࡅධࢀᛶ࠾ࡼࡧ⾜ᨻୖࡢ฼⏝ࡢྍ⬟ᛶ ♫఍ⓗཷࡅධࢀᛶ㸸ᮏヨ㦂ἲࡣ RhCE ࡟ᑐࡍࡿ໬Ꮫ≀㉁ࡢ⣽⬊ẘᛶࢆᣦᶆ࡟⏝࠸࡚║่⃭ᛶࢆホ౯ࡍࡿヨ㦂ἲ࡛࠶ࡾࠊ⏕ࡁࡓື≀ࢆ⏝࠸࡞࠸࡜࠸࠺Ⅼ࡛ࠊ3Rs ࡢ⢭⚄࡟ྜ⮴ࡋ࡚࠸ࡿࠋࡲࡓࠊLabCyte CORNEA-MODEL24 ࡣẚ㍑ⓗᏳ౯࡛࠶ࡾࠊࡑࡢධᡭࡣᐜ࡛᫆ࠊ▷᫬㛫࡛ᐇ᪋࡛ࡁࡿࠋࡲࡓࠊ≉Ṧ࡞ᶵᮦࡸヨ⸆ࢆᚲせ࡜ࡏࡎࠊᚲせ࡞ᡭᢏࡶ」㞧࡞ࡶࡢ࡛࡞࠸ࠋࡋࡓࡀࡗ࡚ࠊධᡭࡋࡓ LabCyte CORNEA-MODEL24 ࡀရ㉁ᇶ‽ࡢチᐜ⠊ᅖ࡟࠶ࡾࠊ࠿ࡘᐇ᪋ࡍࡿヨ㦂᪋タࡢᢏ⾡⩦ᚓࡀ࢞࢖ࢻࣛ࢖ࣥࡢ⩦⇍ᗘ☜ㄆ≀㉁࡛☜࠿ࡵࡽࢀ࡚࠸ࢀࡤࠊᇶᮏⓗ࡞⣽⬊ᇵ㣴ࡢᢏ⾡࡜タഛࢆ᭷ࡍࡿ᪋タ࡛ᐇ᪋ྍ⬟࡛࠶ࡾࠊᢏ⾡⛣㌿ᛶࡣ㧗࠸ࠋ௨ୖࡼࡾࠊᮏヨ㦂ἲࡢ♫఍ⓗཷࡅධࢀᛶࡣ㧗࠸ࠋ ⾜ᨻୖࡢ฼⏝ᛶ㸸ᮏヨ㦂ἲࡣࠊ໬Ꮫ≀㉁࡟ࡼࡿ║่⃭ᛶࢆホ౯࡛ࡁࠊ࣎ࢺ࣒࢔ࢵࣉ᪉ᘧ࡟࠾࠸࡚ UN GHS ༊ศእ≀㉁ࢆ᳨ฟࡍࡿ᪉ἲ࡜ࡋ࡚ࠊ⾜ᨻⓗ฼⏝ࡀྍ⬟࡛࠶ࡿ࡜⪃࠼ࡿࠋཧ⪃ᩥ⊩1) OECD (2017) Guideline for the testing of chemicals. 492, Reconstructed human Cornea-like Epithelium(RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation orserious eye damage.
2) OECD (2016) Performance standards for the assessment of proposed similar or modified in vitro reconstructed
human cornea-like epithelium (RhCE) test methods for identifying chemicals not requiring classification andlabelling for eye irritation or serious eye damage, based on the validated reference methods EpiocularTM EITand SkinethicTM HCE EIT described in TG492.
3) LabCyte Validation Management Team (2017) Me-too Validation Report: Validation Study for LabCyte
CORNEA-MODEL24 Eye Irritation Test (Version. 3.0).
4) Peer Review Panel (2017) Validation status of the LabCyte CORNEA-MODEL24 EYE IRRITTION TEST,
Report of the Peer Review Panel on a JaCVAM co-ordinated study programme addressing the validation statusof LabCyte CORNEA-MODEL24 EYE IRRITATION TEST for discriminating eye irritant from non-eye
irritant substances.
5) OECD (2018) Test Guideline 492. Reconstructed human Cornea-like Epithelium (RhCE) test method foridentifying chemicals not requiring classification and labelling for eye irritation or serious eye damage.
6) JaCVAM ║่⃭ᛶヨ㦂㈨ᩱ⦅⧩ጤဨ఍㸸ホ౯ሗ࿌᭩ ෌ᵓ⠏ࣄࢺゅ⭷ᵝୖ⓶ࣔࢹࣝἲ (RhCEἲ)
LabCyte CORNEA-MODEL24║่⃭ᛶヨ㦂㸦LabCyte CORNEA-MODEL24 EIT㸧㸦2018ᖺ12᭶27᪥㸧55