FOR CANCER RESEARCHERS

THIS IS UNDERSTANDING

AT THE SPEED OF LIFE

Phenoptics™ Research Solutions

Every cancer holds aworld of discovery
Developing effective cancer
immunotherapies – treatments that might
harness the body’s own immune system tofight cancer – could potentially deliver
what the oncology research community
has sought for decades –­ a cure. And toadvance this research, it’s critical that you
see everything a tumor has to show you.
This is because better visualization andidentification of biomarkers and immune
checkpoints lead to improved
understanding of the underlying biologythat lets cancer thrive.
That’s what makes our Phenoptics
instrumentation and workflow such a
powerful solution. The Phenoptics
workflow – multiplex
immunohistochemistry staining solutions,multispectral imaging systems, andadvanced image-analysis software –enables a more comprehensive andspecific view and analysis of theinteraction between tumor and theimmune system, at a cellular level, in asingle tissue section.
Better quantification of cancer-immune
interactions may reveal which diseasemechanisms are in play, and help
researchers discover biomarkers that may
eventually lead to better subpopulation
stratification methodologies.
Simply put, that means a better
understanding of biology that drives
disease. And isn’t that the goal we’re all
striving for?

UNDERSTANDING

STARTS RIGHT HERE

Built around Mantra™ and Vectra® imaging instruments,
Opal™ Multiplex IHC kits, and inForm® image analysis
software, the Phenoptics workflow enables you to quickly
visualize and identify cell types in the context of thetumor, measure expression levels of key proteins related
to immune evasion, determine cellular functional andactivation states, and discover and confirm immune
evasion mechanisms. Want a workflow that enables abetter understanding of the complex interplay between
the immune system and cancer? Let’s get started. . .

IMMUNOSTAIN
The seven colors of discovery
Opal Multiplex IHC kits make multiplex results
accessible to anyone who works with standard
immunohistochemistry, permitting enhanced
visualization and understanding of complex cellularinteractions. With Opal, you can select antibodies for
simultaneous IHC detection based on performance rather
than species. And Opal kits are optimized for reliable
spectral unmixing and simultaneous measurement of three
to six IHC targets, plus a nuclear stain.
Opal enables you to:

Opal IHC works with FFPE tissue and is compatible with standard
IHC workflows.

• Measure three to six tissue biomarkers simultaneously• Use the best primary antibodies, regardless ofspecies — with no cross-reactivity• Identify multiple cell phenotypes while retaining spatial
and morphological context that is lost with bulk
measurements and flow cytometry• Get more information from precious and scarce samples

With our Opal Automation IHC kits you can now
perform Opal multiplex staining on one of the leading
research automated staining platforms – the BOND RX
by Leica Biosystems. Automation provides you with theflexibility to support the dynamic demands oftranslational research.

You can use the best primary antibodies together in multiplex
panels, with no species-based crosstalk.

• Quality, consistency, and reproducibility withevery sample• High throughput protocol - perform 7-color
immunofluorescence staining on 30-slides in 14 hours• Simplified, walk-away protocol versus laborius
manual process

Because you retain spatial cellular context, you get more
information from your precious samples.

IMAGE
Reveal complex biology in asingle tissue section
For a deeper understanding of disease, you need faster, better
visualization and identification of disease biomarkers. And quickening
the pace of that understanding is the whole idea behind Mantra andVectra imaging instruments. These systems enable multiplexed
immune-cell and expression-profiling assays, for tissue-segmentationand immune-cell phenotyping within the tumor and its
microenvironment in FFPE tissues and TMAs.
Mantra Quantitative Pathology Workstation:• Compact, easy-to-use workstation with an intuitive manual
workflow optimized for the research pathologist• Detects and measures multiple expressed and overlapping
biomarkers within a single IHC or IF tissue section
Mantra Quantitative Pathology Workstation• Pathology Views™, feature of inForm image analysis software,
displays fluorescence imagery in traditional brightfield mode
(H&E, DAB)
Vectra 3 Automated Quantitative Pathology Imaging System:• Fully automated, high-throughput imaging system with six or 200
slide configuration• Navigate around slides to identify areas of interest for highresolution multispectral acquisition with the Phenochart™
whole-slide viewer• Detect and measure multiple weakly expressed and overlapping
biomarkers within a single H&E, IHC or IF tissue section and in TMAs
• Automatic identification of specific tissue types using integrated
inForm analysis software

Vectra 3 Automated Quantitative Pathology Imaging System

Vectra Polaris Automated Quantitative Pathology
Imaging System:• Fully automated, high throughput multispectral imaging system
combined with true brightfield and fluorescent whole slide
scanning functionality• Fast and efficient digital whole slide scanning speeds, comparable
to standalone systems• On-the-fly, continuous slide loading allows unlimited throughput• Flexible data analysis, compatible with many image analysis
software platforms

Vectra Polaris Automated Quantitative Pathology Imaging System

ANALYZE AND
UNDERSTAND
Discovery comes withseeing cell-to-cell interactions
Our patented automated inForm® imageanalysis software allows you to accurately
visualize, analyze, and quantify biomarkers intissue sections. It enables accurate per-cell
quantification of specific biomarkers definedwithin multiple tissue contexts, and thephenotyping of immune and other cells in situ
in solid tissue. What’s more, it enables thequantitation of weakly expressing andoverlapping biomarkers within cells and cellularcompartments that can’t be identified by thenaked eye. These sensitive approaches give you
the confidence to discover indicators of disease
and uncover relationships between specific celltypes, and between the immune system andthe tumor. The software:AB• Pathology Views™ feature renders immunofluorescence(IF) images as simulated H&E or DAB andhematoxylin, to provide views more familar to youC• Separates weakly expressing andoverlapping markers• Enables cellular analysis of H&E, IHC, and IF in FFPE
tissue sections• Automatically classifies cell phenotypes usingmachine learning algorithms• inForm Tissue Finder™ automates the detection andsegmentation of specific tissues through patented
pattern recognition algorithms• inForm Cell Analysis™ enables quantitative
per cell analysis of IHC, IF, and RNA-ISH stainingDA) Spectral Unmixing; B) Tissue Segmentation; C) Cell Segmentation;D) Cell Phenotyping

Phenotyping of immune cells and cancer cells within the context of the tumor enables advanced analytics like distance mapping.

PERKINELMER PHENOPTICS RESEARCH SERVICES
We take understanding to the next level
Want to test the Phenoptics workflow before bringing the capability inhouse? The PerkinElmer Research Services team can perform multiplexed IHC
for up to seven colors (six markers plus DAPI) to help you get results. Our
Opal staining method allows all antibodies in a seven-color panel to be
selected based on performance and specificity, without consideration ofspecies of origin. Multispectral imaging provides quantitative results regardlessof spectral and spatial overlap among fluorescence markers, enabling tissue
segmentation, cellular phenotyping, protein expression, and spatial analysis.

Phenoptics Research Services Laboratory

We follow a detailed staining protocol when working with your precious
samples: Antibody specificity is first confirmed in single-plex with positivecontrols. Then the multiplex panel is tested with the same positive controls,with study samples that you provide – so you’re sure the protocol works foryou. Then we agree on staining performance levels, including confirmation ofmultiplex staining independence and non-interference. Analysis begins withmultispectral imaging, providing quantitative spectral unmixing of eachfluorophore signal and tissue autofluorescence, followed by tissue
segmentation and cell phenotyping. This complete workflow enables new
depths of understanding not achievable with standard chromogenic
monoplex or duplex IHC methods.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2016-2017, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.
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