Basic information of commonly used vectors(12/6/2019, GenScript)#Vector NameSpecies

Major application

CopyOrigin

1pBlueScript II KS(-)

Bacterium

Bacterial Protein ExpressionHigh

pUC ori

2pBlueScript II KS(+)

Bacterium

Bacterial Protein ExpressionHigh

pUC ori

3pBlueScript II SK(-)

Bacterium

Bacterial Protein ExpressionHigh

pUC ori

4pBlueScript II SK(+)

Bacterium

Bacterial Protein ExpressionHigh

pUC ori

5pBlueScript SK(+)

Bacterium

Bacterial Protein ExpressionHigh

pUC ori

6pCDFDuet-1

Bacterium

Bacterial Protein ExpressionLow

CDF ori

7

pCOLADuet-1

Bacterium

Bacterial Protein ExpressionLow

ColA ori

8pColdII

Bacterium

Bacterial Protein Expression

Low

ColE1 ori

9pET-11aBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop10

pET-11b

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop11

pET-11c

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop12

pET-11d

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop13

pET-14bBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

14

pET-15bBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

15

pET-16bBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

16

pET-17b

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop17

pET-19b

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop18

pET-20b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop19

pET-21a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop20

pET-21b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop21

pET-21d(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop22

pET-22b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop23

pET-23a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop24

pET-24a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop25

pET-24b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop26

pET-24c(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop27

pET-24d(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop28

pET-25b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop29

pET-26b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop30

pET-27b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop31

pET-28a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop32

pET-28b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop33

pET-28c(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop34

pET-29a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop35

pET-29b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop36

pET-29c(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop1

37

PET-30a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

38

PET-30b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

39

PET-30c(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

40

PET-31b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop41

pET-32a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop42

pET-32b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

43

pET-3aBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

44

pET-3bBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop45

pET-3cBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

46

pET-3dBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

47

pET-41a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

48

pET-41b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop49

pET-41c(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop50

pET-42a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop51

pET-42b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

52

pET-42c(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

53

pET-43.1a(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop54pET-43.1b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

55

pET-45b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

56

pET-50b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

57

pET-51b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

58

pET-52b(+)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop59

pET-9aBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

60

pETDuet-1

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

61

pGEX-2TK

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

62

pGEX-4T-1

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

63

pGEX-4T-1-H(RBS)

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

64

pGEX-4T-1-M(RBS)

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

65

pGEX-4T-2

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

66

pGEX-4T-3

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

67

pGEX-5X-1

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

68

pGEX-5X-1-H(RBS)

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

69

pGEX-5X-1-M(RBS)Bacterium

Bacterial Protein Expression

HighpBR322 ori

70

pGEX-5X-2

Bacterium

Bacterial Protein Expression

HighpBR322 ori

71

pGEX-5X-3

Bacterium

Bacterial Protein Expression

HighpBR322 ori

72

pGEX-6P-1

Bacterium

Bacterial Protein Expression

HighpBR322 ori

73

pGEX-6P-1-H(RBS)

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

74

pGEX-6P-1-M(RBS)

Bacterium

Bacterial Protein ExpressionHigh

pBR322 ori

75

pGEX-6P-2

Bacterium

Bacterial Protein Expression

HighpBR322 ori

76

pGEX-6P-3

Bacterium

Bacterial Protein Expression

HighpBR322 ori277pGS-21a

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

78

pMAL-c4X

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

79

pMAL-c4x-H(RBS)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop80

pMAL-c4X-M(RBS)

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop81

pMAL-c5E

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop82

pMAL-c5X

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

83

pMAL-p5E

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop84

pMAL-p5G

BacteriumBacterial Protein Expression

Low

pBR322 ori+Rop85

pMAL-p5X

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

86

pQE-1

Bacterium

Bacterial Protein Expression

Low

colE1 ori

87

PQE-30Bacterium

Bacterial Protein Expression

Low

colE1 ori

88

pQE-32Bacterium

Bacterial Protein Expression

Low

colE1 ori

89

pQE-60Bacterium

Bacterial Protein Expression

Low

colE1 ori

90

pRSFDuet-1

Bacterium

Bacterial Protein ExpressionHigh

RSF ori91

pAcG2T

Baculovirus

Insect Protein Expression

Low

ColE ori

92

pAcGHLT A

Baculovirus

Insect Protein Expression

Low

ColE ori

93

pAcGHLT B

Baculovirus

Insect Protein Expression

Low

ColE ori

94

pAcGHLT C

Baculovirus

Insect Protein Expression

Low

ColE ori

95

pAcHLT A

Baculovirus

Insect Protein Expression

Low

ColE ori

96

pAcHLT B

Baculovirus

Insect Protein Expression

Low

ColE ori

97

pAcHLT C

Baculovirus

Insect Protein Expression

Low

ColE ori

98

pAcSG2Baculovirus

Insect Protein Expression

Low

ColE ori

99

pBAC-1

Baculovirus

Insect Protein ExpressionHigh

pUC ori

100

pBacPAK8Baculovirus

Insect Protein Expression

HighpUC ori

101

pBacPAK9Baculovirus

Insect Protein ExpressionHigh

pUC ori102

pFastBac-Dual

Baculovirus

Insect Protein ExpressionHigh

pUC ori103

pFastBac1

Baculovirus

Insect Protein ExpressionHigh

pUC ori104

pFastBacHT-A

Baculovirus

Insect Protein ExpressionHigh

pUC ori

105

pFastBacHT-B

Baculovirus

Insect Protein ExpressionHigh

pUC ori106

pFastBacHT-C

Baculovirus

Insect Protein ExpressionHigh

pUC ori

107

pcDNA3.1/V5-His A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

108

pcDNA3.1/V5-His B

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

109

pcDNA3.1(-)

Mammalian

Mammalian Protein ExpressionHighpUC ori

110

pcDNA3.1(-)-myc-His A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori111

pcDNA3.1(-)-myc-His B

Mammalian

Mammalian Protein ExpressionHigh

pUC ori112

pcDNA3.1(-)-myc-His C

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

113

pcDNA3.1(+)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

114

pcDNA3.1(+)-myc-His A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

115

pcDNA3.1(+)-myc-His B

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

116

pcDNA3.1(+)-myc-His C

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

3117

pcDNA3.1/Hygro(-)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

118

pcDNA3.1/Hygro(+)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

119

pcDNA3.1/V5-His C

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

120

pcDNA3.1/Zeo(-)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

121

pcDNA3.1/Zeo(+)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

122

pcDNA3.1(+)-C-6His

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

123

pcDNA3.1(+)-C-DYK

Mammalian

Mammalian Protein ExpressionHigh

pUC ori124

pcDNA3.1(+)-C-DYK-P2A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

125

pcDNA3.1(+)-C-HA

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

126

pcDNA3.1(+)-C-Myc

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

127

pcDNA3.1(+)-N-6His

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

128

pcDNA3.1(+)-N-DYK-P2A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

129

pcDNA3.1(+)-N-GST(TEV)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

130

pcDNA3.1(+)-N-GST(Thrombin)

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

131

pcDNA3.1(+)-N-HA

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

132

pcDNA3.1(+)-N-Myc

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

133

pcDNA3.1(+)-C-eGFP

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

134

pcDNA3.1(+)-N-eGFP

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

135

pcDNA3.1(+)-P2A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

136

pcDNA3.1(+)-P2A-eGFP

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

137

pcDNA3.1(+)-N-DYK

Mammalian

Mammalian Protein ExpressionHighpUC ori

138

pcDNA5/FRT

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

139

pcDNA5/TO

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

140

pcDNA6/V5-His A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

141

pCI-Neo

Mammalian

Mammalian Protein ExpressionHigh

pBR322 ori

142

pCMV-3Tag-1a

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

143

pCMV-3Tag-1a-P2A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

144

pCMV-3Tag-2a

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

145

pCMV-3Tag-3a

Mammalian

Mammalian Protein ExpressionHigh

pUC ori146

pCMV-3Tag-3a-P2A

Mammalian

Mammalian Protein ExpressionHigh

pUC ori147

pCMV-3Tag-4a

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

148

pEGFP-N1

Mammalian

Mammalian Protein ExpressionHigh

pUC ori149

pGen2.1

Mammalian

Mammalian Protein Expression

Low

colE1 ori

150

pGL3-Basic

Mammalian

Mammalian Protein Expression

Low

colE1 ori

151

pGL3-Control

Mammalian

Mammalian Protein Expression

Low

colE1 ori

152

pGL3-Promoter

Mammalian

Mammalian Protein Expression

Low

colE1 ori

153

pGL4.10[luc2]

Mammalian

Mammalian Protein Expression

Low

colE1 ori

154

pGL4.14[luc2/Hygro]

Mammalian

Mammalian Protein Expression

Low

colE1 ori

155

pGL4.17[luc2/Neo]

Mammalian

Mammalian Protein Expression

Low

colE1 ori

156

pHLsecMammalian

Mammalian Protein Expression

Low

colE1 ori

4157

pAO815

PichiaYeast Protein ExpressionHigh

pBR322 ori

158

pPIC3.5K

PichiaYeast Protein ExpressionHigh

pBR322 ori

159

pPIC9

PichiaYeast Protein ExpressionHigh

pBR322 ori

160

pPIC9K

PichiaYeast Protein ExpressionHigh

pBR322 ori

161

pPICZA

PichiaYeast Protein Expression

HighpUC ori

162

pPICZalphaA

PichiaYeast Protein ExpressionHigh

pUC ori

163

pPICZalphaB

PichiaYeast Protein ExpressionHigh

pUC ori

164

pPICZalphaC

PichiaYeast Protein ExpressionHigh

pUC ori

165

pPICZB

PichiaYeast Protein ExpressionHigh

pUC ori

166

pPICZC

PichiaYeast Protein ExpressionHigh

pUC ori167

pESC-HIS

S. cerevisiae

Yeast Protein ExpressionHigh

pUC ori

168

pESC-LEU

S. cerevisiae

Yeast Protein ExpressionHigh

pUC ori169

pESC-TRP

S. cerevisiae

Yeast Protein ExpressionHigh

pUC ori170

pESC-URA

S. cerevisiae

Yeast Protein ExpressionHigh

pUC ori

171

pETCON(-)

S. cerevisiae

Yeast Protein ExpressionHigh

pUC ori

172

pYES2

S. cerevisiae

Yeast Protein ExpressionHigh

pUC ori

173

pUC57

BacteriumCloningHigh

pUC ori

174

pUC57-Kan

Bacterium

CloningHigh

pUC ori

175

pUC57-Simple

Bacterium

CloningHigh

pUC ori

176

pUC57-mini

Bacterium

CloningHigh

pUC ori

177

pUC18

Bacterium

CloningHigh

pUC ori

178

pUC19

Bacterium

CloningHigh

pUC ori179

pCC1

Bacterium

Cloning

Low

F-factor replicon

180

pJET1.2

Bacterium

Cloning

High

pMB1

181

pUC57-Brick

Bacterium

CloningHigh

pUC ori

182

pDONR207

BacteriumCloningLow

pUC ori

183

pDONR221

Bacterium

CloningHigh

pUC ori184

pDONR223

Bacterium

CloningHigh

pUC ori

185

pEntr/D-TOPO

BacteriumCloningHigh

pUC ori

186

pENTR1A

BacteriumCloningHigh

pUC ori

187

pENTR3C

Bacterium

CloningHigh

pUC ori

188

pACYCDuet-1

Bacterium

Bacterial Protein Expression

Low

P15A ori

189

pET-24a(+)-TEVBacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

190

pET-28a(+)-TEV

Bacterium

Bacterial Protein Expression

Low

pBR322 ori+Rop

191

pGenLenti

Mammalian

Mammalian Protein ExpressionHigh

pUC ori

192

pGenDONR

BacteriumCloningHighpUC ori

193

pcDNA3.4

Mammalian

Mammalian Protein ExpressionHigh

pUC ori5