ISSN 2185-6699

秋田県総合食品研究センター報告
第13 号

平成23年 (2011年)

Bulletin of the Akita ResearchInstitute of Food and Brewing(ARIF)
No. 13, 2011             㧝㧚⪺⺰ᢥ㧔ႎᢥ㧕

 ⋡

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̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖

㧞㧚ේ⪺⺰ᢥ㧔⎇ⓥࡁ࡯࠻㧕

1̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 31㧟㧚✚⺑ ̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 39

㧠㧚․⸵ߩ᭎ⷐ

̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 55㧡㧚ቇળ⊒⴫ⷐᣦ

̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 59㧢㧚ᄖㇱ⊒⴫⺰ᢥ᭎ⷐ̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 79

㧣㧚╙ 1 ภ㨪╙ 13 ภੱฬ⚝ᒁ̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 87

㧤㧚⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ႎ๔ⷙቯ

̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 90㧝㧚ේ⪺⺰ᢥ㧔ႎᢥ㧕Ԙ‫ޟ‬0+45 ߦࠃࠆ⣖ⴊᵹᄌൻ㊂ߦၮߠߊ༵ᅢᕈ್ᢿ‫ ̖̖̖̖̖̖̖̖̖̖̖̖ޠ‬1   ᾢ⼱᣽ೣԙ‫⫾ޟ‬㙃ߦࠃࠆ㓽ࡂ࠲ࡂ࠲ߩຠ⾰଻ᜬᛛⴚߩ㐿⊒‫ ̖̖̖̖̖̖̖̖̖̖̖̖ޠ‬7   Ⴆᧄ⎇৻‫ᧁ⦁ޔ‬ീ㧖  㧔㧖⑺↰⋵ㄘᨋ᳓↥ᛛⴚ࠮ࡦ࠲࡯࡮᳓↥ᝄ⥝࠮ࡦ࠲࡯㧕Ԛ‫ޟ‬ᷡ㈬߽ࠈߺ߳ߩ♧ᷝടࠍ೑↪ߒߚᣂⷙ㈬ㅧᛛⴚߩᬌ⸛‫ ̖̖̖̖̖̖̖̖ޠ‬17
ᄢ㊁೰‫ޔ‬㜞ᯅੳ‫ᧄ᧖ޔ‬ാੱ‫↰ޔ‬ญ㓉ାԛ‫ࠍ☨⦡᦭ޟ‬ේᢱߣߒߚᣂⷙ↞๧⚛᧚ߩ⹜૞‫ ̖̖̖̖̖̖̖̖̖̖̖̖̖̖ޠ‬24
㊄ሶ㓉ብ

熊谷昌則

秋田県総合食品研究センター報告

Vol. 13, １−６（2011）㧺㧵㧾㧿ߦࠃࠆ⣖ⴊᵹᄌൻ㊂ߦၮߠߊ㘩ຠߩ༵ᅢᕈ್ᢿᾢ⼱᣽ೣ㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧕
Masanori KUMAGAI ‫ޤ⚂  ⷐޣ‬NIRS㧔Near Infra- Red Spectroscopy㧕ߪ‫ޔ‬ᵄ㐳 700㨪1000nm ߩㄭ⿒ᄖశ߇↢૕⚵❱߳ߩㅘㆊᕈ߇޽ࠅ‫⚛㉄ߩࡦࡆࡠࠣࡕࡋ߽߆ߒޔ‬ൻ⁁ᘒߦࠃߞߡ‫ޔ‬శߩๆ෼࡮ᢔੂߩ
ᐲว޿߇ᄌൻߔࠆߎߣࠍ೑↪ߒߡ‫ޔ‬㗡⊹਄߆ࠄ㕖ଚⷅ⊛ߦ⣖ᯏ⢻ࠍࡑ࠶ࡇࡦࠣߔࠆ
ᣇᴺ⺰ߢ޽ࠆ㧝㧕‫⎇ᧄޕ‬ⓥߩ⋡⊛ߪ‫ࠆ޽ޔ‬㘩ຠࠍ⷗ߡ‫ߩߘޔ‬ᄖⷰ߆ࠄ߅޿ߒߘ߁߆ߤ
߁߆‫ޔ‬㘩ߴߚ޿߆ߤ߁߆‫༵ߚߞ޿ߣߤߥޔ‬ᅢᕈ߿ᗵᕈᖱႎࠍ‫ޔ‬ญ㗡ߥߤߢᗧᕁ⴫␜
ߐߖࠆߎߣߥߊ‫ޔ‬NIRS ߢ᷹ቯߒߚ⣖ⴊᵹᄌൻࠍᜰᮡߣߒߡ⣖߆ࠄ⋥ធ‫ߺ⺒ޔ‬ขࠇߥ޿߆ߤ߁߆ࠍᬌ⸽ߔࠆߎߣߢ޽ࠆ‫⚿ߩߘޕ‬ᨐ‫ޔ‬㘩ຠߩᄖⷰ⹏ଔᤨߦ߅ߌࠆⵍ㛎⠪ߩ༵ᅢㆬᛯߩ㆑޿߇೨㗡೨㊁ਔ஥ㇱ⴫ጀߩ⣖ⴊᵹ㊂ߦ૗ࠄ߆ߩᓇ㗀ࠍ෸߷ߒߡ޿ࠆ
ߎߣ߇␜ໂߐࠇߚ߇‫ߩߘޔ‬ᔕ╵ߦߪ୘ੱᏅ߇޽ࠅ‫ⵍࠆߥ⇣ޔ‬㛎⠪㑆ߢ߽᥉ㆉ⊛ߥᕈ⾰ࠍ⷗޿ߛߔߦߪ޿ߚࠄߥ߆ߞߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬ห৻ߩⵍ㛎⠪ౝߢ᭴▽ߐࠇߚ್೎ࡕ࠺࡞ࠍ૶߃߫‫ⵍߩߘޔ‬㛎⠪୘ੱ߇ߤ߁ᗵߓߡ޿ࠆ߆ߤ߁߆ࠍ੍᷹ߢ߈ࠆน⢻ᕈ߇޽ࠆ‫ޕ‬‫ޤ⸒  ✜ޣ‬㘩ຠߩ㐿⊒ߦ߅޿ߡ㊀ⷐߣߥࠆ‫߿ߐߒ޿߅ޔ‬ḩ⿷ᗵߥߤߣ޿ߞߚᔃℂ⊛࿃ሶߩ⹏
ଔᴺߣߒߡߪ৻⥸ߦ‫⺞࠻࡯ࠤࡦࠕޔ‬ᩏᴺ߿ቭ⢻ᬌᩏᴺߥߤߦࠃࠆ⾰໧⚕ᴺ߇↪޿ࠄࠇࠆ‫ߩߎޕ‬ᣇᴺߢߪ‫༵ޔ‬ᅢᕈ߿ᗵᕈߦଥࠊࠆࡅ࠻ߩᔕ╵߇‫ޔ‬ญ㗡߹ߚߪ⸥ㅀߦࠃࠅ
᛽಴ߐࠇࠆ‫༵ޔ߈ߣߩߎޕ‬ᅢ߿ᗵᕈᖱႎߣ޿ߞߚ߽ߩߪ‫ޔ‬ᒻኈ⹖߿ᒻኈേ⹖ߦࠃߞ
ߡ᣿␜⊛ߦ⴫⃻ߐࠇࠆ‫ߥ߁ࠃߩߎޕ‬᣿␜⊛⹏ଔߪ‫ޔ‬ቴⷰᕈ‫ޔ‬ౣ⃻ᕈ‫ޔ‬ା㗬ᕈߥߤߦᰳߌࠆ႐ว߇޽ࠅ‫ߩߘޔ‬ઍᦧᴺߣߒߡ‫↢ޔ‬ℂቇ⊛ᜰᮡ‫߫߃଀ޔ‬ᔃᜉᢙ‫ⴊޔ‬࿶‫ޔ‬૕᷷‫ޔ‬⊒᳨㊂‫⴫ߚ߹ߪ޿ࠆ޽ޔ‬ᖱ߿ߒߋߐߥߤߣ޿ߞߚേ૞ᜰᮡࠍ↪޿ߚ㕖᣿␜⊛ᚻᴺߥߤ߽ᬌ⸛ߐࠇߡ޿ࠆ㧞㧕‫ޕ‬╩⠪ࠄߪߎࠇ߹ߢ‫ޔ‬㘩ຠߩฃኈᕈ߿༵ᅢᕈ‫ߪߦࠄߐޔ‬㘩ຠࠍ៨ขߒߚ㓙ߩḩ⿷ᐲ߿≹ߒᗵߥߤࠍቴⷰ⊛ߦ್ᢿߒ߁ࠆᣂߒ޿㘩ຠ⹏ଔᴺߩ㐿⊒ࠍ⋡ᜰߒߡ‫ޔ‬NIRS㧔ㄭ⿒ᄖಽశᴺ㧦Near Infra- Red Spectroscopy㧕ߦ⌕⋡ߒ‫ࠍࠇߎޔ‬ㆡ↪ߔߴߊ‫ޔ‬⒳‫ߩޘ‬ขࠅ⚵ߺߢታ↪ൻߩߚ߼ߩᬌ⸽ࠍ㊀ߨߡ߈ߚ㧟㧘㧠㧕‫ޕ‬NIRS ߢߪ‫ޔ‬⣖ౝߩ␹⚻ᵴേ߇⿠ߎߞߡ޿ࠆ㗔ၞߩࡋࡕࠣࡠࡆࡦߩỚᐲᄌൻࠍ㗡⊹਄߆ࠄ㕖ଚⷅ⊛ߦ⸘᷹ߔࠆߎߣ߇
ߢ߈ࠆ‫ޕ‬NIRS ߪ f-MRI 㧔functional㧦ᯏ⢻⊛㧙Magnetic Resonance Imaging㧦⏛᳇౒㡆↹௝㧕ߥߤߣ޿ߞߚઁߩ⣖ᯏ⢻⸘᷹ᴺߦᲧセߒߡ‫ޔ‬ૐ᜔᧤⸘᷹ߢ޽ࠆߚ߼‫ޔ‬ㅧᓇ
−1−೷ߩᛩਈߥߤ߽ਇⷐߢ‫ߚ߹ޔ‬ᐳ૏ߥߤߢ߽⸘᷹น⢻ߥߎߣ߆ࠄⵍ㛎⠪ߩ⽶ᜂ߇ᭂ┵ߦዋߥ޿ߣ޿߁೑ὐ߇޽ࠆ‫ޕ‬⣖⴫ጀߩⴊᵹᄌൻߒ߆᷹ቯߢ߈ߥ޿‫ޔ‬ⓨ㑆ಽ⸃⢻߇ᖡ޿ߥߤߩᰳὐߪ޽ࠆ߽ߩߩ‫ޔ‬ක≮ಽ㊁ߩߺߥࠄߕ‫ޔ‬㕖ක≮ಽ㊁ߢߩᔕ↪߽ᕆỗߦ᜛߇ߞߡ޿ࠆ‫ޕ‬ᧄ⎇ⓥߢߪ‫ޔ‬NIRS ࠍ㘩ຠ⹏ଔᴺߩᣂⷙઍᦧᚻᴺߣߒߡߩߺߥࠄߕ‫ޔ‬ㄭᐕ‫ޔ‬ᵈ⋡ࠍ㓸߼ߡ޿ࠆ࠾ࡘ࡯ࡠ࡮ࡑ࡯ࠤ࠹ࠖࡦࠣ㧔␹⚻ࡑ࡯ࠤ࠹ࠖࡦࠣ߹ߚߪᔃ⣖ࡑ࡯ࠤ࠹ࠖࡦࠣ㧕㧡‫ޔ‬㧢㧕߳ߩዷ㐿߽⷗ᝪ߃ߡ‫ߩߘޔ‬ၮ␆⊛⍮⷗ࠍ⫾Ⓧߒߚ޿ߣ⠨߃ߡ޿ࠆ‫࠾ޕ‬ࡘ࡯ࡠ࡮ࡑ࡯ࠤ࠹ࠖࡦࠣߢߪ‫ߦࠣࡦࠖ࠹ࠤ࡯ࡑޔ‬⣖ᯏ⢻⸘᷹ߩᚻᴺࠍขࠅ౉ࠇࠆߎ
ߣߦࠃߞߡ‫ޔ‬ᶖ⾌⠪ᔃℂ߿ⴕേࠬ࠲ࠗ࡞ߩ⸃᣿ߦߟߥ߇ࠅ‫ޔ‬ᶖ⾌⠪ߩᧄ㖸߿ήᗧ⼂ਅߩ෻ᔕ߇ᒁ߈಴ߖࠆߩߢߪߥ޿߆ߣᦼᓙߐࠇߡ޿ࠆ‫ޕ‬੹࿁ߪ‫ޔ‬೨ႎ㧠㧕ߦᒁ߈⛯߈‫ޔ‬᭽‫ߥޘ‬㘩ຠ↹௝ࠍⷞⷡೝỗߣߒߡਈ߃‫↹ߩߘޔ‬௝⹏ଔᤨߩ೨㗡೨㊁ߦ߅ߌࠆዪᚲ⣖ⴊᵹᄌൻ㊂߆ࠄ‫ⵍޔ‬㛎⠪ߩ༵ᅢ߿ᗵᕈᖱႎࠍ㕖᣿␜⊛ߦ‫ޔ‬⣖߆ࠄ⋥ធ‫ߺ⺒ޔ‬ขࠇߥ޿߆ߤ߁߆ᬌ⸛ߒߚߣߎࠈ‫⧯ޔ‬ᐓߩ⍮⷗߇ᓧࠄࠇߚ
ߩߢએਅߦႎ๔ߔࠆ‫ޕ‬‫ޣ‬ᣇ  ᴺ‫ޤ‬ⵍ㛎⠪ߪ‫࠮ࡦࠦ࠼ࡓ࡯ࠜࡈࡦࠗޔ‬ࡦ࠻ߩᚻ⛯߈߇ߣࠄࠇߚ‫ޔ‬ஜᏱߥฝ೑߈ߩ 20㨪40 ઍߩᅚᕈߢ޽ࠅ‫ߘޔ‬ߩ߁ߜᧄ⎇ⓥߢߪ 8 ฬ㧔ᐔဋᐕ㦂
33.6 ᱦ㧕ߩ࠺࡯࠲ߦߟ޿ߡ⸃ᨆࠍⴕ
ߞߚ‫ⵍޕ‬㛎⠪ߦߪ‫ޔ‬࿑㧝ߦ␜ߔࠃ߁
ߦ‫ޔ‬17 ࠗࡦ࠴ߩ CRT ਄ߦ⒳‫ߩޘ‬㘩ຠ↹௝ 2 ⒳㘃ࠍ 1 ⚵ߣߒߡ๒␜ߒ‫ޔ‬ೋ߼ߦ‫৻߆ࠄߜߤޔ‬ᣇࠍ㗡ߩਛߢ༵ᅢㆬᛯߐߖߚᓟߦ‫੹ޔ‬ᐲߪ㧝⒳㘃ߕ‫׋‬ᾀᴾ Ꮿᘉ්ἙὊἑᚘยỉಮ‫܇‬ᴾߟ↹௝ࠍ๒␜ߒ㧔࿑㧞㧕‫ࠍࠇߙࠇߘޔ‬࠲ࠬࠢߣߒߡ 15 ⑽㑆‫ޔ‬ᵈⷞߐߖߚ‫ߩߘޕ‬ᓟ‫↹ߩࠄߜߤޔߦߜ⋥ޔ‬௝ࠍㆬᛯߒߚߩ
߆㧔ߤߜࠄߣ߽޿߃ߥ޿ࠍ฽߻㧕‫⺞࠻࡯ࠤࡦࠕޔ‬ᩏߦࠃࠅႎ๔ࠍ᳞߼ߚ‫ࠍⴕ⹜ߩߎޕ‬⇣ߥࠆ↹௝ߩ⒳㘃 10 ⚵ߦኻߒߡ➅ࠅ㄰ߒታᣉߒߡ‫ޔ‬10 ಽ㑆ߩ቟㕒ભᙑࠍᝌ౉ߒߚ
ᓟ‫ߦࠄߐޔ‬೎ߩ↹௝ 10 ⚵ߦኻߒߡห᭽ߩ⹜ⴕࠍታᣉߒߚ‫ޕ‬⣖ⴊᵹᄌൻ㊂ߪశ࠻ࡐ‫׋‬ᾁᴾ ဒ΂ỉ੩ᅆ૾ඥểἑἋἁᴾ
−2−ࠣ ࡜ ࡈ ࠖ ࡯ ETG-4000( ᣣ ┙ ࡔ࠺ࠖࠦ㧕ࠍ↪޿ߚ NIRS ߦࠃࠅ‫ޔ‬Ꮐฝ೨㗡ㇱߦ߅ߌࠆ 33
㧔12ch㧕2 ࡕ࡯࠼ߢ᷹ቯߒߚ‫ޕ‬࿑㧟ߦ‫⸘ޔ‬᷹ㇱ૏ߣ ch ⇟ภࠍ␜ߔ‫ޔߚ߹ޕ‬࿑㧠ߦߪขᓧ࠺࡯
࠲ߩ৻଀ߣߒߡ‫ⵍޔ‬㛎⠪㧭ߩ↹௝Ԙߦኻߔࠆ ch8 ߩ Oxy-Hb ᄌൻ㊂ߩផ⒖ࠍ␜ߒߚ‫⸃࠲࡯࠺ޕ‬ᨆߪ‫৻߆ࠄߜߤޔ‬ᣇߩ༵ᅢㆬᛯ
ߐࠇߚ↹௝ࠍ⷗ߡ޿ࠆߣ߈ߩ࠲
ᴾ
ࠬࠢਛߣ‫↹޿ߥߪߢ߁ߘޔ‬௝ࠍ‫׋‬ᾂᴾ ᚘยᢿˮể ᶁᶆ ဪӭᴾ⷗ߡ޿ࠆߣ߈ߩ࠲ࠬࠢਛߩ
Oxy-Hb ᄌൻ㊂ࠍ‫ޔ‬ᱜⷙൻಣℂ㨇 Z-score㧩㧔㧔↹௝ឭ␜ᓟ 5 ⑽㨪15 ⑽߹ߢߩᄌൻ㊂ߩᐔဋ㧕㧙㧔0 ⑽㨪5 ⑽߹ߢߩᐔဋᄌൻ㊂㧕㧕㧛㧔0 ⑽㨪5 ⑽ߩᄌൻ㊂ߩᮡḰ஍Ꮕ㧕㧕㨉ᓟߦᐔဋ୯ߩᏅߩᬌቯ߿್೎
⸃ᨆߦଏߒߚ‫ޕ‬‫׋‬ᾃᴾ ᵭᶖᶗᵋᵦᶀ ‫҄٭‬᣽ỉਖ਼ᆆ̊ίᘮ᬴ᎍᾐẆဒ΂ṞẆᵡᶆᵖὸᴾ

‫⚿ޣ‬ᨐߣ⠨ኤ‫ޤ‬ߪߓ߼ߦ‫ⵍߩࠇߙࠇߘޔ‬㛎⠪ߩ೨㗡ㇱߦ㈩⟎ߒߚᏀฝ 12ch ߩ⸘ 24ch ߩ Z-scoreࠍ↪޿ߡ‫৻߆ࠄߜߤޔ‬ᣇߩ༵ᅢㆬᛯߐࠇߚ↹௝ࠍ⷗ߡ޿ࠆߣ߈ߩ࠲ࠬࠢਛߣ‫߁ߘޔ‬ߢߪߥ޿↹௝ࠍ⷗ߡ޿ࠆߣ߈ߩ࠲ࠬࠢਛߩ Oxy-Hb ᄌൻ㊂ߦ㆑޿߇޽ࠆ߆ߤ߁߆ࠍ
ߘࠇߙࠇߩ ch Ფߦ t ᬌቯߢ⹏ଔߒߚߣߎࠈ‫ⵍޔ‬㛎⠪ 8 ฬߩ߁ߜ 6 ฬߦߟ޿ߡߪ‫ޔ‬ෂ㒾₸ 5%ߢ᦭ᗧᏅߩ⹺߼ࠄࠇࠆ ch ߇ሽ࿷ߒߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬24ch ߩ߁ߜ 7chߢ᦭ᗧᏅߩ⹺߼ࠄࠇߚⵍ㛎⠪߇޿ࠆ৻ᣇߢ‫᦭ޔ‬ᗧᏅ߇⹺߼ࠄࠇߚߩߪ 1ch ߩߺߣ޿߁ⵍ㛎⠪߽ 3 ฬ޿ߚ㧔ⵍ㛎⠪ A‫ޔ‬F‫ޔ‬G㧕㧔࿑㧡㧕‫ⵍޕ‬㛎⠪㑆ߢ৻⥌ߒߡ‫․ޔ‬ቯߩ ch㧔߹ߚߪ㗔ၞ㧕ߦࠃࠆ㆑޿ߪ⹺߼ࠄࠇߕ‫ޔ‬୘ੱᏅ߇ᄢ߈߆ߞߚ‫ޕ‬−3−‫׋‬ᾄᴾ ἑἋἁɶỉᘮ᬴ᎍК ᶁᶆ ൑ỉ ᵭᶖᶗᵋᵦᶀ ‫҄٭‬᣽ỉࠀီίῃ౨‫ܭ‬ὸᴾᰴߦ‫ޔ‬24ch ోߡߩ Z-score ࠍ↪޿ߡ್೎ಽᨆࠍㆡ↪ߒߚߣߎࠈ‫್ޔ‬೎ࡕ࠺࡞᭴▽ᤨߦߪోⵍ㛎⠪ᐔဋߢ 98.1%ߩᱜ╵₸㧔࿑㧡ߦ␜ߐࠇߡ޿ࠆ್೎ࡕ࠺࡞ᱜ╵₸ߩⵍ㛎⠪㑆ᐔဋ㧕ߢ༵ᅢㆬᛯࠍᱜߒߊ್೎ߢ߈ࠆߎߣ߇ࠊ߆ߞߚ‫ޔߜࠊߥߔޕ‬⣖ⴊᵹᄌൻߩ࠺࡯࠲ࠍ૶߃߫‫ⵍޔ‬㛎⠪߇ߤߜࠄߩ↹௝ࠍㆬᛯߒߚߩ߆‫ⵍޔ‬㛎⠪⥄ࠄ߇ญ㗡ߥߤߢ␜ߐߥߊߡ߽࠺࡯࠲਄߆ࠄ್೎ߢ߈ࠆߎߣ߇␜ߐࠇߚࠊߌߢ޽ࠆ‫ޕ‬−4−‫׋‬ᾅᴾ ᘮ᬴ᎍ൑ỉ։‫ڤ‬ᢠ৸ʖยЙКἴἙἽỉᚸ̖ᴾ

ߘߎߢ‫ޔ‬ᰴߩࠬ࠹࠶ࡊߣߒߡ‫ᧂޔ‬⍮⹜ᢱ‫ߜࠊߥߔޔ‬ㆊ෰ߦ⷗ߚߎߣ߇ߥ޿‫ޔ‬ᣂߒ޿↹௝ߦኻߒߡ߽ห᭽ߦ್೎ߢ߈ࠆߩ߆ߤ߁߆ࠍࠢࡠࠬࡃ࡝࠺࡯࡚ࠪࡦ
㧔cross-validation㧕ߦࠃࠅᬌ⸽ߒߚ‫ޔߪߢߎߎޕ‬੤Ꮕᬌቯ㧔leave-one-out㧕ߦࠃࠅ 20 ⚵ߩ↹௝ߩ⚵ߺวࠊߖߩ߁ߜ‫ޔ‬19 ⚵ߩ࠺࡯࠲ࠍᣢ⍮⹜ᢱߣߒߡ್೎ࡕ࠺࡞ࠍ
᭴▽ߒ‫ޔ‬ᱷࠅߩ 1 ⚵ߩ࠺࡯࠲ࠍᧂ⍮⹜ᢱߣߺߥߒ‫ޔ‬వߩ್೎ࡕ࠺࡞ߢᧂ⍮⹜ᢱ߇್೎ߢ߈ࠆ߆ߤ߁߆ࠍᬌ⸽ߒߚ‫ోޕ‬૕ߢߪߎߩᠲ૞ࠍ 1 ⚵ߕߟ㗅ߦ‫ޔ‬20 ⚵ߩ࠺࡯࠲ߦ
ㆡ↪ߒߡ‫್ࠇߙࠇߘޔ‬೎ߢ߈ࠆ߆ߤ߁߆ࠍᬌ⸽ߒߚߣߎࠈ‫ޔ‬࿑㧢ߦ␜ߒߚࠢࡠࠬࡃ
−5−࡝࠺࡯࡚ࠪࡦᱜ╵₸ߩోⵍ㛎⠪ᐔဋߪ‫ޔ‬45.6%ߣߥߞߚ‫߽ᦨޕ‬ᱜ╵₸߇㜞޿ⵍ㛎⠪H ߢ޽ߞߡ߽ 66.7%ߦߣߤ߹ߞߚ‫ޕ‬એ਄ߩ⚿ᨐ߆ࠄ‫ޔ‬㘩ຠ↹௝ೝỗߦኻߔࠆᄖⷰ⹏ଔᤨߦ༵ᅢㆬᛯߩ㆑޿߇೨㗡೨㊁ਔ஥ㇱ⴫ጀߩ⣖ⴊᵹ㊂ߦ૗ࠄ߆ߩᓇ㗀ࠍ෸߷ߒߡ޿ࠆߎߣ߇␜ໂߐࠇߚ߇‫ߪࠇߘޔ‬⇣ߥࠆⵍ㛎⠪㑆ߢ᥉ㆉ⊛ߥᔕ╵ߣߪ޿߃ߥ߆ߞߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬ห৻ߩⵍ㛎⠪ౝߢ᭴▽ߐࠇߚ್೎ࡕ࠺࡞ࠍ૶߃߫‫ⵍߩߘޔ‬㛎⠪୘ੱߩ༵ᅢ߿ᗵᕈᖱႎࠍ㕖᣿␜⊛ߦ᛽಴ߢ߈ࠆน⢻ᕈ߇޽ࠆ‫♖ࠆߥࠄߐޔߒߛߚޕ‬ᐲะ਄߇ᔅⷐߢ޽ࠅ‫ޔ‬ታ㛎ᣇᴺ߿࠺
࡯࠲⸃ᨆᴺߩᡷༀ߇᳞߼ࠄࠇࠆ‫ޕ‬‫  ⻢ޣ‬ㄉ‫ޤ‬ታ㛎ߦߏዧജ޿ߚߛ޿ߚ⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ߩ੗਄߹߈ሶߐࠎ‫ޔ‬ർᨋદㇺሶߐࠎ‫ޔ‬ᷓ੗⌬ሶߐࠎߦ‫ⵍߡߒߘޔ‬㛎⠪ߣߒߡߏෳട޿ߚߛ޿ߚ⊝᭽ᣇߦᷓ⻢ߔࠆ‫⎇ᧄޔߚ߹ޕ‬ⓥߩ৻ㇱߪ⑼⎇⾌㧔22500751㧕ߩഥᚑࠍฃߌߡታᣉߐࠇߚ‫ޕ‬‫ޣ‬ᢥ  ₂‫ޤ‬㧝㧕Jobsis FF㧦Noninvasive infrared monitoring of cerebral and myocardial
oxygen sufficiency and circulatory parameters㧘 Science, 198: 1264-1267
㧔1977㧕㧚
㧞㧕ᣣ⑼ᛛㅪቭ⢻ᬌᩏᆔຬળ✬㧦ቭ⢻ᬌᩏࡂࡦ࠼ࡉ࠶ࠢᣂ 㧘ᣣ⑼ᛛㅪ಴ ␠㧘
(1973)㧚
㧟㧕ᾢ⼱ࠄ㧦⣖ᯏ⢻⸘᷹ߦࠃࠆᣂߒ޿㘩ຠߩ⹏ଔᴺߩ㐿⊒㧘⑺↰⋵✚ว㘩ຠ⎇ⓥᚲ
ႎ๔ 10 ภ㧘29-36 (2008)㧚
㧠㧕ᾢ⼱ࠄ㧦㘩ຠߩᄖ༵ⷰᅢ⹏ଔᤨߦ߅ߌࠆ೨㗡೨㊁ዪᚲ⣖ⴊᵹേᘒߩ⸃ᨆ㧘⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ႎ๔ 12 ภ㧘1-6 (2010)㧚
㧡㧕‫ޟ‬⣖ࠍᵴ߆ߔ‫⎇ޠ‬ⓥળ㧦ࡉ࡟ࠗࡦ࡮࠺ࠦ࡯࠺ࠖࡦࠣ̆⣖ᖱႎࠍ⺒߻̆㧘ࠝ࡯ࡓ␠㧘
㧔2007㧕㧚
㧢㧕A࡮K࡮ࡊ࡜࠺ࠖ࡯ࡊ㧦ࡑ࡯ࠤ࠲࡯ߩ⍮ࠄߥ޿‫ޟ‬95%‫ޠ‬ᶖ⾌⠪ߩ‫޿ߚ޿⾈ޟ‬㧍‫ޠ‬ࠍ૞ࠅ಴ߔታ〣⣖⑼ቇ㧘㒋ᕆࠦࡒࡘ࠾ࠤ࡯࡚ࠪࡦ࠭㧘㧔2011㧕㧚


−6−

塚本研一

秋田県総合食品研究センター報告

Vol. 13, ７− 16（2011）⫾㙃ߦࠃࠆ㓽ࡂ࠲ࡂ࠲ߩຠ⾰଻ᜬᛛⴚߩ㐿⊒Ⴆᧄ⎇৻ ઁ㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯‫⋵↰⑺ ޔ‬ㄘᨋ᳓↥ᛛⴚ࠮ࡦ࠲࡯᳓↥ᝄ⥝࠮ࡦ࠲࡯㧕
Ken-ichi TSUKAMOTO, Tsutomu FUNAKI㧖‫ޤ⚂ ⷐޣ‬ 㓽ࡂ࠲ࡂ࠲ߩ㐳ᦼຠ⾰଻ᜬࠍ⋡⊛ߣߒߡ⫾㙃ߦࠃࠆᣇᴺࠍᬌ⸛ߒߚ‫ޕ‬㓽ࡂ࠲ࡂ࠲ߩຠ⾰਄㊀ⷐߢ޽ࠆෆ႙ᚑಽࠍຠ⾰଻ᜬߩᜰᮡߣߒߡ⹜㛎ࠍⴕߞߚ⚿ᨐ‫࠲ࡂ࠲ࡂޔ‬ෆ႙ߪᒻᘒ⊛ߦߪ⫾㙃㧞ㅳ㑆‫ޔ‬ෆ႙ߩടᾲᤨߩ☼ࠅߢߪ⫾㙃㧠ㅳ㑆ߢ߽ຠ⾰߇⛽ᜬߢ߈ࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ‫ޕ‬‫ޤ⸒ ✜ޣ‬ࡂ࠲ࡂ࠲ߩṪ₪߇⍴ᦼ㓸ਛဳߢ޽ࠅṪ₪㓸ਛᤨߦ㝼ଔߩૐਅ߇⪺ߒ޿ߣ޿߁໧㗴ὐ߇޽ࠆ‫ޕ‬ㅢᏱ‫࠲ࡂ࠲ࡂޔ‬㞲㝼ߢߩຠ⾰଻ᜬߪ಄⬿଻▤߿ᵹㅢ⁁ᘒ߇ᦨ⦟ߢ޽ߞߡ
߽ 1 ㅳ㑆⒟ᐲ߇㒢⇇ߢ޽ࠆߎߣ‫ޔߚ߹ޔ‬಄ಓ㝼ߣߒߚ႐วߪ‫࠲ࡂ࠲ࡂޔ‬ෆᧄ᧪ߩ㘩ᗵ߇ᄬࠊࠇ‫ޔ‬㓽ࡂ࠲ࡂ࠲ߣߒߡߩ໡ຠଔ୯߇ᭂᐲߦૐਅߔࠆߎߣ߇⍮ࠄࠇߡ޿ࠆ‫ޕ‬ᧄ⎇ⓥߢߪ‫ߩࠄࠇߎޔ‬໧㗴ὐࠍ⸃᳿ߔࠆߎߣࠍ⋡⊛ߣߒߡ‫⁁ߚ߈↢ࠍ࠲ࡂ࠲ࡂޔ‬ᘒߢ 2㨪3 ㅳ㑆ή⛎㙄⫾㙃ߔࠆߎߣߦࠃࠅຠ⾰ࠍ଻ᜬߒߚ߹߹಴⩄ᤨᦼ⺞ᢛ߇น⢻ߢ޽ࠆ߆ࠍᬌ⸛ߔࠆߎߣߣߒߚ‫⫾ޕ‬㙃ߣߪ㝼੺㘃ࠍ↢߈ߚ⁁ᘒߢṪ₪ߒߚᓟ‫ޔ‬಴⩄೨ߩ৻ቯߩᦼ㑆↢߈ߚ⁁ᘒߢ଻ᜬߒᏒ႐ߦଏ⛎ߔࠆᛛⴚߢ޽ࠅ‫ޔ‬㞲ᐲ଻ᜬߩ㧝ߟߩᣇ
ᴺߢ޽ࠆ‫ޕ‬㝼㘃ߩ⛘㘩߿㘫㙈ਛߩ㝼૕ᚑಽᄌേߪ⢽⾰ᚑಽ╬ 1),2)ߦߟ޿ߡ⺞ߴࠄࠇ‫ޔ‬↢๮⛽ᜬߩࠛࡀ࡞ࠡ࡯Ḯߣߥߞߡ޿ࠆߣႎ๔ߐࠇߡ޿ࠆ߇‫ޔ‬ᚑᾫߒߚෆᎽߩᚑಽᄌ
േߦ㑐ߒߡߪ⺞ߴࠄࠇߡ޿ߥ޿‫⎇ᧄޕ‬ⓥߢߪή⛎㙄⫾㙃ߦ߅ߌࠆෆᎽᚑಽߩᄌേࠍ
ਛᔃߦಽᨆߒ‫ޔ‬㓽ࡂ࠲ࡂ࠲ߩຠ⾰਄㊀ⷐߥෆᎽᚑಽ߇⫾㙃ߢຠ⾰଻ᜬ߇น⢻ߢ޽ࠆ߆ᬌ⸛ߒߚ‫ޕ‬‫ޣ‬ᣇ ᴺ‫ޤ‬㧝㧚⹜ᢱ
1)⫾㙃ࡂ࠲ࡂ࠲⹜ᢱ
 ⑺↰⋵↵㣮Ꮢߩർᶆ࿾వߩࡂ࠲ࡂ࠲ቯ⟎✂ߢṪ₪ߐࠇߚ㧟㨪㧠ᱦߩቢᾫࡂ࠲
ࡂ࠲㓽‫ޔ‬㓶ࠍᵴ㝼ߩ⁁ᘒߢ‫ޔ‬หᏒߩ⑺↰⋵᳓↥ᝄ⥝࠮ࡦ࠲࡯ߩ᳓ᮏߦ៝౉ߒ⫾㙃⹜㛎ߦଏߒߚ‫ޕ‬2)ಽᨆ⹜ᢱ 㝼⡺ߪណෆᓟಓ⚿଻ሽߒߚࡂ࠲ࡂ࠲ฦ 10 የࠍ⸃ಓߒ൮ৼߢ 3 ᨎߦ߅ࠈߒ‫⊹ޔ‬ઃ߈ߩ⁁ᘒߢ㝼⡺ㇱಽࠍ⸃೬↪ࡂࠨࡒߢ⚦߆ߊೞࠎߛ߽ߩࠍ੃㋬ߢဋ৻ߦ⏴⎈ᷙวߒಽᨆ⹜ᢱߣߒߚ‫ߚ߹ޕ‬ෆᎽ‫♖ޔ‬Ꮍߪฦ 5 የಽࠍ㝼⡺ห᭽ߦಣℂߒߚ߽ߩಽ
ᨆ⹜ᢱߣߒߚ‫ޕ‬㧞㧚⫾㙃⹜㛎
᳓ᮏߪ᳓↥ᝄ⥝࠮ࡦ࠲࡯ታ㛎᫟ߦ⸳⟎ߒߚ 1 ࠻ࡦ౞ᒻࡄࡦ࡜ࠗ࠻᳓ᮏࠍ૶↪ߒ‫ޔ‬−7−ฦ⹜㛎඙ 150 የࠍ⫾㙃ߒߚ‫ޕ‬㘺⢒᳓ߪࠈㆊᶏ᳓ࠍ 1 ᤨ㑆ᒰߚࠅ 0.25 ຿㨪0.3 ຿ࠍᎼᵹ߇↢ߓߥ޿ࠃ߁᳓ᮏ਄ 1 ࡨᚲ߆ࠄᵈ᳓ߒ‫ޔ‬ㅢ᳇ߪࠛࠕ࡯ࠬ࠻ࡦ 1 ୘ࠍ↪޿ᓸㅢ᳇ߣߒߚ‫ޕ‬㓽ߣ㓶ߩᲧ₸ߩ㆑޿ߦࠃࠆ⫾㙃ߩ↢ᱷ₸ࠍᲧセߔࠆߚ߼㓽 100%ߩ⫾㙃㧔એਅ A ඙ߣߔࠆ㧕‫ޔ‬㓽 70%ߣ㓶 30%ߩ⫾㙃㧔એਅ B ඙ߣߔࠆ㧕‫ޔ‬㓽 50%ߣ㓶 50%ߩ⫾㙃㧔એਅ C ඙ߣߔࠆ㧕߅ࠃ߮㓽 30%ߣ㓶 70%ߩ⫾㙃㧔એਅ D ඙ߣߔࠆ㧕ࠍ 12 ᦬ਛᣨ߆ࠄ⠉ᐕ 1 ᦬਄ᣨ߹ߢߩ 4 ㅳ㑆ή⛎㙄ߢⴕߞߚ‫ޔߚ߹ޕ‬ฦᚑಽߩಽᨆ↪⹜ᢱߦߟ޿ߡߪ A ඙ߢฦ 10 የࠍ 1 ㅳ㑆Ფߦ 4 ㅳ㑆߹ߢࠍ᳓ᮏ߆ࠄណขߒ⥄ὼᱫߐߖߡ⹜ᢱ
ߣߒߚ‫ޔߚ߹ޕ‬ᚑಽᲧセߩߚ߼㓶 100%ߩ 50 የߢή⛎㙄⫾㙃ࠍⴕ޿‫ޔ‬ฦ 5 የࠍ 2ㅳ㑆Ფߦ 4 ㅳ㑆߹ߢណขߒห᭽ߦ⹜ᢱߣߒߚ‫ޕ‬㧟㧚ಽᨆ⹏ଔᣇᴺ
1)᳓ಽ
᳓ಽߪಽᨆ⹜ᢱ 5㨓ࠍᏱ࿶ടᾲੇ῎ᴺ 3)ߢ 105͠‫ޔ‬3 ᤨ㑆ടᾲੇ῎ߒߚੇ῎ᷫ㊂ࠃࠅ▚಴ߒߚ‫ޕ‬2)☻⢽⾰฽㊂ ☻⢽⢌฽㊂ߪ⹜ᢱ 20㨓ࠍࠢࡠࡠࡎ࡞ࡓ-ࡔ࠲ࡁ࡯࡞ᷙᶧᡷ⦟᛽಴ᴺ 4)ߦࠃࠅಽ
ᨆߒߚ‫ޕ‬
3)⢽⢌㉄⚵ᚑ☻⢽⢌฽㊂᷹ቯߩߚ߼᛽಴ߒߚ⢽⾰ࠍ 1N ᳓㉄ൻࠞ࡝࠙ࡓߢࠤࡦൻߒㆆ㔌⢽⢌㉄ࠍ⺞⵾ߒ‫࡞࠹ࠬࠛ࡞࠴ࡔࠅࠃߦࡦ࠲ࡔ࠱ࠕࠫ࡞࡝ࠪ࡞࠴ࡔ࡝࠻ࠍࠇߎޔ‬ൻߒߡࠟࠬࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ಽᨆߦଏߒߚ‫ޔߜࠊߥߔޕ‬⢽⢌㉄⚵ᚑಽᨆߪࠟࠬࠢࡠࡑ
࠻ࠣ࡜ࡈ㧔HEWLETT PACKARD ␠⵾㧘GC5890ΤPlus㧕ࠍ૶↪ߒ‫ޔ‬ᬌ಴ߪ FID‫ޔ‬ࠞ ࡜ ࡓ ߪ WCOT FUSED SILICA coating CP-SIL88 Tailor made FAME
㧔0.25mmI.D.×50m L.,0.2Ǵm thin, ;Varian ␠⵾㧕ࠍ૶↪ߒߚ‫ࡓ࡜ࠞޕ‬᷷ᐲߢߪ
150-200͠ߪ 2͠/min ߩ᣹᷷‫ޔ‬200-225͠ߪ 5͠/min ߩ᣹᷷ߢ 225͠ࠍ 8 ಽ଻ᜬߣߒߚ‫ߚ߹ޕ‬ᬌ಴ེ᷷ᐲߪ 250͠‫ ߪࠬࠟ࡯ࠕ࡝ࡖࠠޔ‬He㧔1ml/min㧕‫࠶࡝ࡊࠬޔ‬࠻Ყߪ 10㧦1 ߩ᧦ઙߢಽᨆߒߚ‫ޕ‬⢽⢌㉄⚵ᚑߪᮡḰ⢽⢌㉄ࡔ࠴࡞ࠛࠬ࠹࡞㧔PUFA
No.3, from Menhaden Oil ;SUPELCO ␠⵾㧕߆ࠄฦ⢽⢌㉄ࠍหቯߒߡ▚಴ߒߚ‫ޕ‬4)ㆆ㔌ࠕࡒࡁ㉄ ⹜ᢱ 5㨓ࠍ 10㧑ㆊႮ⚛㉄ߢ᛽಴ߒ‫╬ޔ‬㊂ߩ 0.02N Ⴎ㉄ṁᶧࠍട߃Ꮧ㉼ߒߚᓟ‫ޔ‬
0.45m ߩࡈࠖ࡞࠲࡯ߢࠈㆊߒߡㆆ㔌ࠕࡒࡁ㉄㊂ࠍಽᨆߒߚ‫ޕ‬ㆆ㔌ࠕࡒࡁ㉄ಽᨆ
ߪࠕࡒࡁ㉄ಽᨆ⸘㧔ᣣᧄ㔚ሶ JLC500㧕ߦଏߒߚ‫ޕ‬ಽᨆߪࠞ࡜ࡓߦ㓁ࠗࠝࡦ੤឵
᮸⢽ LCR-7㧔4mmI.D.×70mm L.㧕ࠍ૶↪ߒߚ↢૕ࠕࡒࡁ㉄ಽᨆࡕ࡯࠼ߢ‫ࡒࠕޔ‬ࡁ㉄ߩᬌ಴ߪ࠾ࡦࡅ࠼࡝ࡦᴺ‫⹜ޔ‬ᢱᵈ౉㊂ߪ 50l ߩ᧦ઙߢⴕߞߚ‫ޕ‬
5)☼⾰‛㊂ ࡂ࠲ࡂ࠲ෆ႙߆ࠄߩ☼⾰‛ߩಽ㔌ߪ⪺⠪ࠄߩᣇᴺ 5)ߦࠃࠅⴕߞߚ‫⫾ߜࠊߥߔޕ‬㙃⹜㛎ߩ᳓ᮏ߆ࠄណขߒߚ㓽ࡂ࠲ࡂ࠲ 5 የ߆ࠄ 2 ᤨ㑆એౝߦណෆߒߚෆ႙ࠍ㧝୘
ߕߟ 70͠ߩ⚐᳓ 200ml ߦᠣᜈߒߥ߇ࠄᶐẃߒߚ‫ޕ‬ᶐẃᓟ 2㨪3 ಽߦಽ㔌ߒߚ☼⾰‛ߣෆ☸ࠍ࿁෼ߒ‫⌀ޔ‬ⓨಓ⚿ੇ῎ߒߡ㊀㊂ࠍ᷹ቯߒߚ‫‛⾰☼ޕ‬㊂ߩ⚻ᤨᄌൻߪෆ
႙ 10 ୘ว⸘ߩੇ῎㊀㊂ᒰߚࠅߩੇ῎☼⾰‛ߩᲧ㧔☼⾰‛ੇ῎㊀㊂㧛ෆ㧗☼⾰‛ੇ῎㊀㊂㧕ࠍ▚಴ߒᲧセߒߚ‫ޕ‬
−8−

6)☼⾰‛ SDS-PAGE ☼⾰‛㊂ࠍ᷹ቯߒߚ⫾㙃ฦㅳߩੇ῎☼⾰‛⹜ᢱߦߟ޿ߡ㔚᳇ᵒേಽᨆࠍⴕߞ
ߚ‫⹜῎ੇߜࠊߥߔޕ‬ᢱ 5mg/ml ߩỚᐲߢ⹜ᢱᏗ㉼ᶧ 6)ߦṁ⸃ߒ 100͠ߢ 3 ಽടᾲߒ SDS ൻࠍⴕߞߚ‫ޕ‬12.5%ࠥ࡞Ớᐲߩ㔚᳇ᵒേ↪ࠥ࡞㧔ࠕ࠻࡯⵾ NPU-12.5L ࡄࠫࠚ࡞㧕ࠍ↪޿‫ޔ‬200V‫ޔ‬20mA‫ޔ‬1.5 ᤨ㑆ߩ᧦ઙߢ㔚᳇ᵒേࠍⴕߞߚ‫ޕ‬ᬌ಴ߪࠢࡑࠪ࡯ࡉ࡝࡝ࠕࡦ࠻ࡉ࡞࡯R250㧔Biorad ⵾㧕ߦࠃࠅᨴ⦡ߒߡⴕߞߚ‫߅ߥޕ‬ኻᾖߪ⫾㙃೨ߩ㓽ࡂ࠲ࡂ࠲ෆ႙߆ࠄ⺞⵾ߒߚੇ῎☼⾰‛ࠍ૶↪ߒߚ‫ޕ‬7)⫾㙃ࡂ࠲ࡂ࠲ෆ႙ߩቭ⢻⊛⹏ଔ ⫾㙃 0‫ޔ‬2 ߅ࠃ߮ 4 ㅳߩ㓽ࡂ࠲ࡂ࠲ฦ 2 የࠍ 10%ߩ㘩Ⴎ᳓ߦ 30 ಽ㑆ᶐẃߒ‫ޔ‬200͠ߩࠣ࡝࡞ߢ὾߈㝼ߣߒߡ⹜㘩ߒߚ‫ޕ‬ቭ⢻⊛⹏ଔߪࡊࡠࡈࠔࠗ࡞ᴺߦࠃࠅෆ႙ߩ☼ࠅߣ㝼⡺ߩ㘩ᗵ‫ޔ‬๧ࠍᲧセߒߚ‫ߪ࡞ࡀࡄޕ‬ᒰ⎇ⓥᚲ᳓↥ടᎿᜂᒰ⠪ࠍ฽߻㧡ฬߣߒߚ‫ޕ‬ߥ߅‫⹜࠲ࡂ࠲ࡂޔ‬ᢱಽᨆ⚿ᨐߩᢙ୯ߪ n=3 ߩᐔဋ୯ߢ␜ߒߚ‫ޕ‬‫ ⚿ޣ‬ᨐ‫ޤ‬㧝㧚⫾㙃⹜㛎↢ᱷ₸ฦ⹜㛎඙ߩ㓽ࡂ࠲ࡂ࠲ߩ↢ᱷ₸ࠍ࿑ 1 ߦ␜ߒߚ‫ޕ‬A ඙ߢߪ‫⫾ޔ‬㙃 4 ㅳߢߩ↢ᱷ₸
96.1%ߣߥࠅ⹜㛎඙ਛᦨ߽㜞޿↢ᱷ₸ߢ޽ߞߚ‫ޕ‬B ඙ߪ 65.8%ߢ⫾㙃 3 ㅳએ㒠ߦ㓽ࡂ࠲ࡂ࠲ߩ↢ᱷ₸߇ૐਅߔࠆ௑ะߦ޽ߞߚ‫ޕ‬㧯඙ߪ 18.0%ߢ⫾㙃 2 ㅳએ㒠ߦ㓽ࡂ࠲ࡂ࠲ߩ↢ᱷ₸߇ૐਅߔࠆ௑ะߦ޽ߞߚ‫ޕ‬㧰඙ߪ 6.6%ߢ㧯඙ห᭽ߦ⫾㙃 2 ㅳએ㒠ߦ↢ᱷ₸߇ૐਅߔࠆ௑ะߦ޽ࠅ‫⹜ޔ‬㛎඙ਛߢߪᦨ߽ૐ޿↢ᱷ₸ߢ޽ߞߚ‫ޔ߅ߥޕ‬㓶ࡂ࠲ࡂ࠲ߩ↢ᱷ₸ߪ 100%ߢ޽ߞߚ‫ޕ‬࿑㧝 ⫾㙃⹜㛎ߩࡂ࠲ࡂ࠲↢ᱷ₸㧞㧚ࡂ࠲ࡂ࠲㝼⡺߅ࠃ߮↢ᱺᎽߩ᳓ಽߣ⢽⾰฽㊂ߩᄌേ ⫾㙃㓽ࡂ࠲ࡂ࠲ߩ㝼⡺߅ࠃ߮↢ᱺᎽߩ᳓ಽ‫ޔ‬⢽⾰฽㊂ߩᄌേࠍ⴫ 1 ߦ␜ߒߚ‫߹ޕ‬ߚ‫ޔ‬Ყセኻᾖߣߒߡ㓶ࡂ࠲ࡂ࠲ߩ⚿ᨐࠍ⴫ 2 ߦ␜ߒߚ‫ޕ‬㓽ࡂ࠲ࡂ࠲ߩ㝼⡺᳓ಽߪ⫾㙃ᦼ㑆߇㐳ߊߥࠆߣჇടߒ 0 ㅳ 79.1%߆ࠄ 4 ㅳߢ 85.6%‫☻ޔ‬⢽⢌㊂ߪᷫዋߒ 0 ㅳ−9−

3.2%߆ࠄ 4 ㅳߢ 1.0%ߣߥߞߚ‫ޕ‬㓶ࡂ࠲ࡂ࠲߽ห᭽ߩ௑ะࠍ␜ߒ‫ޔ‬㝼⡺᳓ಽߪ 0 ㅳ
80.0%߆ࠄ 4 ㅳߢ 84.1%‫☻ޔ‬⢽⢌㊂ߪ 0 ㅳ 3.2%߆ࠄ 4 ㅳߢ 0.5%ߣߥߞߚ‫☻ޕ‬⢽⢌㊂ߦ㑐ߒߡߪ㓶ࡂ࠲ࡂ࠲㝼⡺ߩᷫዋ߇⪺ߒ߆ߞߚ‫৻ޕ‬ᣇ‫↢ޔ‬ᱺᎽߪෆᎽߢ㝼⡺ߣห᭽ߩ௑ะࠍ␜ߒ‫ޔ‬0 ㅳ 78.4%߆ࠄ 4 ㅳߢ 81.3%‫☻ޔ‬⢽⢌㊂ߪᷫዋߒ 0 ㅳ 3.3%߆ࠄ 4
ㅳߢ 1.5%ߣߥߞߚ‫♖ޔߒ߆ߒޕ‬Ꮍߪ߹ߞߚߊ௑ะ߇⇣ߥࠅ 2 ㅳߢᦨ߽᳓ಽ߇㜞޿୯ߢ☻⢽⢌߇ૐ޿୯ࠍ␜ߒߚ߇‫♖ߪࠇߎޔ‬Ꮍߩ㊀㊂ᄌേߣ㑐ㅪߔࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬⴫㧝 ⫾㙃⹜㛎ߩ㓽ࡂ࠲ࡂ࠲ߩ᳓ಽ‫☻ޔ‬⢽⢌฽㊂⴫㧞 ⫾㙃⹜㛎ߩ㓶ࡂ࠲ࡂ࠲ߩ᳓ಽ‫☻ޔ‬⢽⢌฽㊂㧟㧚⫾㙃ࡂ࠲ࡂ࠲㝼⡺߅ࠃ߮↢ᱺᎽ⢽⢌㉄⚵ᚑߩᄌേ ⫾㙃㓽ࡂ࠲ࡂ࠲ߩ㝼⡺߅ࠃ߮↢ᱺᎽߩ⢽⢌㉄⚵ᚑߩᄌേࠍ⴫ 3 ߦ␜ߒߚ‫ޕ‬㝼⡺ߩ⢽⢌㉄⚵ᚑߪᲧ₸߇ᄙ޿⢽⢌㉄ߩࡄ࡞ࡒ࠴ࡦ㉄(C 16:0㧘16.0-17.1㧑) ‫࡟࠻ࡒ࡞ࡄޔ‬ࠗࡦ㉄(C 16:1n7㧘9.5-12.2㧑)‫(㉄ࡦࠗ࡟ࠝޔ‬C 18:1n9㧘27.4-30.0㧑)‫ࡦࡍࠨࠦࠗޔ‬࠲ࠛࡦ㉄(C 20:5n3㧘6.7-10.2㧑) ߅ࠃ߮࠼ࠦࠨࡋࠠࠨࠛࡦ㉄(C 22:6n3㧘6.2-7.6㧑)ߢߪᄌേ߇ዊߐ߆ߞߚ‫⫾ߪ㉄ࡦࠛ࠲ࡦࡍࠨࠦࠗޕ‬㙃ᦼ㑆߇㐳ߊߥࠆߣ߿߿ᷫዋߔࠆ
௑ะߢ޽ߞߚ‫ޕ‬⢽⢌㉄⒳㘃೎ߢߪో㘻๺⢽⢌㉄‫ ోޔ‬1 ଔਇ㘻๺⢽⢌㉄߅ࠃ߮ోᄙଔ
ਇ㘻๺⢽⢌㉄⚵ᚑߪ㓽ࡂ࠲ࡂ࠲㝼⡺ߢᄌേ߇߶ߣࠎߤ⷗ࠄࠇߥ߆ߞߚ‫ ⴫ޕ‬3 ߦ␜ߒߚ࠼ࠦࠨࡋࠠࠨࠛࡦ㉄ߣࠗࠦࠨࡍࡦ࠲ࠛࡦ㉄ߩᲧ₸㧔DHA/IPA㧕ߪ㓽ࡂ࠲ࡂ࠲㝼⡺ߢᄌേ߇߶ߣࠎߤ⷗ࠄࠇߥ߆ߞߚ‫ޕ‬㓽ࡂ࠲ࡂ࠲ෆᎽߩ⢽⢌㉄⚵ᚑߪᲧ₸߇ᄙ޿⢽⢌㉄ߩࡄ࡞ࡒ࠴ࡦ㉄(C 16:0㧘18.7-22.9㧑) ‫(㉄ࡦࠗ࡟࠻ࡒ࡞ࡄޔ‬C 16:1n7㧘5.2-6.8㧑)‫ޔ‬ࠝ࡟ࠗࡦ㉄(C 18:1n9㧘13.4-24.6㧑)‫(㉄ࡦࠛ࠲ࡦࡍࠨࠦࠗޔ‬C 20:5n3㧘14.3-22.4㧑)߅ࠃ߮࠼ࠦࠨࡋࠠࠨࠛࡦ㉄(C 22:6n3㧘18.3-24.7㧑)ߢߪ㝼⡺ߣߪ⇣ߥࠆᄌേࠍ␜ߒ
− 10 −

⴫㧟 ⫾㙃⹜㛎ߩ㓽㝼⡺߅ࠃ߮ෆᎽ⢽⢌㉄⚵ᚑ a ߩᄌേ⴫㧠 ⫾㙃⹜㛎ߩ㓶㝼⡺߅ࠃ߮♖Ꮍ⢽⢌㉄⚵ᚑ a ߩᄌേ− 11 −

ߚ‫⫾ޕ‬㙃ᦼ㑆߇㐳ߊߥࠆߎߣߦࠃࠅࠝ࡟ࠗࡦ㉄ߪჇട௑ะ‫߅㉄ࡦࠛ࠲ࡦࡍࠨࠦࠗޔ‬ࠃ߮࠼ࠦࠨࡋࠠࠨࠛࡦ㉄ߪᷫዋ௑ะߢ޽ߞߚ‫ޕ‬⢽⢌㉄⒳㘃೎ߢߪ㝼⡺ߣ⇣ߥࠅో 1ଔਇ㘻๺⢽⢌㉄߇Ⴧടߒోᄙଔਇ㘻๺⢽⢌㉄߇ᷫዋߒߚ߇‫ోޔ‬㘻๺⢽⢌㉄ߪᄌേ߇߶ߣࠎߤ⷗ࠄࠇߥ߆ߞߚ‫ޔߚ߹ޕ‬Ყセኻᾖߣߒߡ㓶ࡂ࠲ࡂ࠲ߩ⢽⢌㉄⚵ᚑߩᄌേࠍ
⴫ 4 ߦ␜ߒߚ‫ޕ‬㓶ࡂ࠲ࡂ࠲㝼⡺ߪ㓽㝼⡺ߣห᭽ߦᄌേ߇ዊߐ߆ߞߚ߇‫ࡦࡍࠨࠦࠗޔ‬࠲ࠛࡦ㉄߅ࠃ߮࠼ࠦࠨࡋࠠࠨࠛࡦ㉄ߪ⫾㙃ᦼ㑆߇㐳ߊߥࠆߣᷫዋߒߚ‫♖ޕ‬ᎽߢߪෆᎽߣ㘃ૃߒߚᄌേࠍ␜ߒ‫⫾ޔ‬㙃ᦼ㑆߇㐳ߊߥࠆߎߣߦࠃࠅࠝ࡟ࠗࡦ㉄ߪჇട௑ะ‫ޔ‬ࠗࠦࠨࡍࡦ࠲ࠛࡦ㉄߅ࠃ߮࠼ࠦࠨࡋࠠࠨࠛࡦ㉄ߪᷫዋ௑ะߢ޽ߞߚ‫ࡋࠨࠦ࠼ߦ․ޕ‬ࠠࠨࠛࡦ㉄ߩᷫዋߪෆᎽߣᲧセߒߡ㗼⪺ߦ⹺߼ࠄࠇߚ‫ޕ‬㧠㧚⫾㙃ࡂ࠲ࡂ࠲㝼⡺߅ࠃ߮↢ᱺᎽߩㆆ㔌ࠕࡒࡁ㉄ߩᄌേ ⫾㙃㓽ࡂ࠲ࡂ࠲ߩ㝼⡺߅ࠃ߮↢ᱺᎽߩㆆ㔌ࠕࡒࡁ㉄ߩᄌേࠍ⴫ 5 ߦ␜ߒߚ‫ޕ‬㝼⡺ߩㆆ㔌ࠕࡒࡁ㉄✚㊂ߪ 82.6-183.2mg/100g ߢ޽ࠅ‫ޔ‬2 ㅳ⋡߇ᦨᄢߢ 3㨪4 ㅳ⋡ߢ߿߿ᷫዋߔࠆ߇⫾㙃㐿ᆎᤨࠃࠅߪᄙߊߥߞߚ‫ࡦ࡝࠙࠲ߚ߹ޕ‬㧔31.5-60.9mg/100g㧕߇ㆆ㔌ࠕࡒࡁ㉄ߩਛߢߪᦨ߽ᄙߊߢ‫ޔ‬ᰴߦࠕࡦ࠮࡝ࡦ㧔0-36.9mg/100g㧕߿ࠛ࠲ࡁ࡯
࡞ࠕࡒࡦ㧔1.8-16.7mg/100g㧕߇ᄙ߆ߞߚ‫㉄ࡦࡒ࠲࡞ࠣޔߚ߹ޕ‬㧔12.7-6.9mg/100g㧕߽ᄙߊ‫⫾ޔ‬㙃ᦼ㑆߇㐳ߊߥࠆߣᷫዋߒߚ‫ࡦ࠾࡜ࠕޕ‬㧔6.1-18.1mg/100g㧕ߪࠣ࡞࠲ࡒࡦ㉄ߣㅒߩ௑ะࠍ␜ߒ‫⫾ޔ‬㙃ᦼ㑆߇㐳ߊߥࠆߣჇടߒߚ‫✚ޕ‬ㆆ㔌ࠕࡒࡁ㉄ߩਛߢ࠲࠙࡝ࡦߣࠕࡦ࠮࡝ࡦߩഀว߇ᄢ߈ߊ‫߇ࠇߎޔ‬ㆆ㔌ࠕࡒࡁ㉄✚㊂ߩᄌേߦᓇ㗀ࠍ෸߷ߒߡ޿ߚ‫ޕ‬ᰴߦෆᎽߩㆆ㔌ࠕࡒࡁ㉄✚㊂ߪ 55.5-133.5mg/100g ߢ޽ࠅ‫ޔ‬3 ㅳ⋡߇ᦨᄢߢ⫾㙃ߦࠃࠅᄙߊߥߞߚ‫ߪࡦ࡝࠙࠲ޕ‬ෆᎽߢ 7.8-21.8mg/100g ࠣ࡞࠲ࡒࡦ㉄ߪ
ෆᎽߢ 3.9-6.0mg/100g‫ ߪࡦ࠾࡜ࠕޔ‬1.7-5.0mg/100g‫ߪࡦ࡝࠮ࡦࠕޔ‬㝼⡺ߣ⇣ߥࠅᬌ಴ߐࠇߥ߆ߞߚ߇‫ࡦࡒࠕ࡞࡯ࡁ࠲ࠛޔ‬㧔26.3-53.4mg/100g㧕߇ᄙ߆ߞߚ‫ޕ‬ෆᎽߢߪࠛ࠲ࡁ࡯࡞ࠕࡒࡦߣ࠲࠙࡝ࡦߩഀว߇ᄢ߈ߊߎࠇ߇ෆᎽߩㆆ㔌ࠕࡒࡁ㉄✚㊂ߩᄌേߦᓇ㗀ࠍ෸߷ߒߡ޿ߚ‫ޕ‬㓶ࡂ࠲ࡂ࠲㝼⡺ߪㆆ㔌ࠕࡒࡁ㉄✚㊂‫߮ࠃ߅ࡦ࡝࠙࠲ޔ‬ࠛ࠲ࡁ࡯࡞ࠕࡒࡦߢ㓽㝼⡺ߣห᭽ߥᄌേ௑ะࠍ␜ߒߚ߇‫ࡦ࡝࠮ࡦࠕޔ‬㊂ߪዊߐ޿୯
ߢ޽ߞߚ‫♖ޕ‬Ꮍߢߪㆆ㔌ࠕࡒࡁ㉄✚㊂ߩᄌേ㧔77.8-529.8mg/100g㧕߇⪺ߒߊ‫⫾ޔ‬㙃ᦼ㑆߇㐳ߊߥࠆߎߣߦࠃࠅㆆ㔌ࠕࡒࡁ㉄✚㊂߇Ⴧടߒߚ‫ࡦࡒࠕ࡞࡯ࡁ࠲ࠛߦ․ޕ‬ߩჇടߪෆᎽߣᲧセߒߡ㗼⪺ߦ⹺߼ࠄࠇߚ‫ޕ‬㧡㧚⫾㙃ෆ႙ߩᒻᘒ⊛ᄌൻ ⫾㙃ࡂ࠲ࡂ࠲ෆ႙ߩᒻᘒ⊛ᄌൻࠍ࿑ 2 ߦ␜ߒߚ‫ޕ‬ෆ႙ߪ⫾㙃 2 ㅳ⋡߹ߢߪᒢജ
ᕈ߇޽ࠅࡏ࡯࡞⁁ߩᒻᘒࠍ଻ߞߡ޿ߚ߇‫ޔ‬3 ㅳ⋡߆ࠄߪෆ႙߇፣ࠇߡ޿ࠆഀว߇Ⴧ
ടߒ‫ޔ‬4 ㅳ⋡ߢߪ࠯࡝࡯⁁‛⾰ߩࠦࠕ߿♻߇ᶖᄬߒߚෆ႙߇ᄙߊ⹺߼ࠄࠇߚ‫ޕ‬એ਄߆ࠄᒻᘒ⊛ߦߪ⫾㙃 2 ㅳ⋡߹ߢ⫾㙃㐿ᆎ߆ࠄᄌൻ߇ߥ޿ߎߣ߇ࠊ߆ߞߚ‫ޕ‬㧢㧚⫾㙃ࡂ࠲ࡂ࠲ෆ႙☼⾰‛㊂ߩᄌേ ࡂ࠲ࡂ࠲ෆ႙ߩ㊀㊂ߪ୘૕Ꮕ߇޽ࠆߚ߼‫‛⾰☼ޔ‬㊂ߩᄌൻࠍಽ㔌ߒߚෆߣߩੇ῎㊀㊂Ყ㧔☼⾰‛㧛☼⾰‛㧗ෆ㧕ߣߒߡ▚಴ߒߚ‫ޕ‬࿑ 3 ߦ⚻ᤨᄌൻࠍ␜ߒߚ߇‫ޔ‬2 ㅳ⋡߹ߢ௖߆ߦᷫዋߒ 3㨪4 ㅳߢᄢ߈ߊᷫዋߒߚ‫ޕ‬
㧣㧚SDS-PAGE ⫾㙃ࡂ࠲ࡂ࠲ෆ႙ߩ☼⾰‛ࠍ SDS㧙PAGE ߢಽᨆߒߚ⚿ᨐ‫ߩߡߴߔޔ‬ㅳߦ߅޿ߡ
න৻ࡃࡦ࠼ߢಽሶ㊂ߪ⚂ 43㨗Da ߢ޽ࠅ‫⫾ޔ‬㙃ߦࠃࠆᄌൻߪ⷗ࠄࠇߥ߆ߞߚ‫ޕ‬
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⴫㧡 ⫾㙃⹜㛎ߩ㓽㝼⡺߅ࠃ߮ෆᎽㆆ㔌ࠕࡒࡁ㉄⚵ᚑ a ߩᄌേ࿑㧞 ࡂ࠲ࡂ࠲ෆ႙ߩᒻᘒ⊛ᄌൻ    − 13 −࿑㧟 ࡂ࠲ࡂ࠲ෆ႙☼⾰‛㊂ߩᄌൻ㧤㧚⫾㙃ෆ႙ߩቭ⢻⊛⹏ଔ ෆ႙ߩቭ⢻⹏ଔߪ 0‫ޔ‬2 ߅ࠃ߮ 4 ㅳߦⴕߞߚ‫⫾ޕ‬㙃㓽ࡂ࠲ࡂ࠲ࠍ 10%ߩ㘩Ⴎ᳓ߦ30 ಽ㑆ᶐẃߒ‫ޔ‬200͠ߩࠣ࡝࡞ߢ὾߈㝼ߣߒߚ‫࠲ࡂ࠲ࡂޕ‬ෆ႙ߪ 0 ㅳߣᲧセߒߡ 2ㅳߢߪห᭽ߥ☼ࠅ߇ᄙߊ޽ࠅ‫ޔ‬ෆߩ㘩ᗵ߽⦟ᅢߢ޽ߞߚ‫ޕ‬4 ㅳߢߪ☼ࠅߪ 0 ㅳߣᲧ
セߒߡ߿߿ഠࠆ߇‫ޔ‬㘩ᗵ⊛ߦ߽චಽߥ☼ࠅ߇޽ࠅ‫ޔ‬ෆߩ㘩ᗵ߽⦟ᅢߢ޽ߞߚ㧔࿑ 4㧕‫ޕ‬߹ߚ‫ޔ‬㝼⡺ㇱߪㅢᏱߩቄ▵㓽ࡂ࠲ࡂ࠲ߣᲧセߒߡ߿߿᳓ಽ߇ᄙߊᨵࠄ߆ߊᗵߓࠄࠇ
ߚ߇‫⟤ޔ‬๧ߒߊ㘩ߴࠆߎߣ߇ߢ߈ߚ‫ޕ‬࿑㧠 ⫾㙃㧠ㅳෆ႙ߩቭ⢻⹏ଔ‫ޣ‬⠨ ኤ‫ޤ‬㧝㧚⫾㙃⹜㛎 㓽ࡂ࠲ࡂ࠲߇ 100%⹜㛎඙ߢߪ⫾㙃 4 ㅳߢ߽↢ᱷ₸ 96.1%ߢ޽ߞߚ߇‫ޔ‬㓶ࡂ࠲ࡂ࠲ᷙ౉₸߇㜞ߊߥࠆߣ㓽ࡂ࠲ࡂ࠲ߩ↢ᱷ₸߇ૐਅߒߚ‫ޔߪߣߎߩߎޕ‬㓶ᷙ౉ߦࠃࠆ
ࠬ࠻࡟ࠬߩჇടߥߤ߇⠨߃ࠄࠇࠆ‫ޔߡߞ߇ߚߒޕ‬ታ㓙ߦ⫾㙃ࠍⴕ߁႐วߪߢ߈ࠆߛ
ߌ‫ޔ‬㓶ࡂ࠲ࡂ࠲ߩᷙ౉ࠍ㒐ߋኻ╷߇ᔅⷐߢ޽ࠆ‫ߪ࠲ࡂ࠲ࡂ᧪ᧄޔߚ߹ޕ‬᳓ᷓ 250m
ઃㄭߩૐ᳓᷷ၞߢ↢ᕷߔࠆ㝼⒳ߢ޽ࠅ‫ޔ‬౻ቄߢ޽ߞߡ߽ᴪጯၞߩ᳓᷷ߪ↢ᕷၞࠃࠅ㜞ߊࠬ࠻࡟ࠬߣߥࠆߎߣ߇ផኤߐࠇࠆ‫⫾࠲ࡂ࠲ࡂޕ‬㙃ߦࠃࠆຠ⾰଻ᜬታ↪ൻࠍ࿑ࠆ႐วߪ‫੍ޔ‬ᗐߐࠇࠆࠬ࠻࡟ࠬࠍน⢻ߥ㒢ࠅ㒰ߊߎߣ߇㊀ⷐߣ⠨߃ࠄࠇࠆ‫ޕ‬㧞㧚⫾㙃ࡂ࠲ࡂ࠲㝼⡺߅ࠃ߮↢ᱺᎽߩᚑಽᄌേ ࡂ࠲ࡂ࠲㞲㝼ߩ☻⢽⢌㊂ߩቄ▵⊛ᄌേߪ‫ޔ‬៨㙄ߩᄙ޿ 9 ᦬ߦ㝼⡺☻⢽⢌㊂߇ᦨᄢߣߥࠆ߇‫ޔ‬ᕈᚑᾫ߇ㅴߺ↥ෆᦼߩ 12 ᦬߹ߢߪ៨㙄߇ߥߊߥࠅ☻⢽⢌㊂ᷫዋߒ‫↢ޔ‬ᱺᎽߩ⊒㆐߇ఝవ⊛ߦ⛽ᜬߐࠇࠆߎߣ߇ႎ๔ߐࠇߡ޿ࠆ 7)‫⫾ᧄޕ‬㙃⹜㛎ߪߎߩ↥ෆᦼߩࡂ࠲ࡂ࠲ࠍߐࠄߦή⛎㙄ߢ⫾㙃ߒߚߎߣߦࠃࠅ‫ߦࠄߐޔ‬㝼⡺☻⢽⢌߇↢๮⛽ᜬߦߚ߼ᶖ⾌ߐࠇߚߣ⠨߃ࠄࠇࠆ‫ޕ‬㝼⡺☻⢽⢌ߩᷫዋߣหᤨߦ↥ෆᦼߩቄ▵⊛ᄌേߢߪ⷗ࠄࠇߥ߆ߞߚෆᎽ☻⢽⢌ߩᷫዋ߽⫾㙃ߢߪ⷗ࠄࠇߚ‫↥ߪߣߎߩߎޕ‬ෆࠃࠅ↢๮⛽ᜬ߇ఝవߐࠇߚߚ߼ߣផኤߐࠇࠆ‫ޕ‬㓶ࡂ࠲ࡂ࠲ߦ߅޿ߡ߽♖Ꮍ㊀㊂ᷫዋߦࠃࠅ↢๮⛽ᜬ߇ఝవߐࠇߚߣ⠨߃ࠄࠇࠆ‫ޕ‬
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㧟㧚⫾㙃ࡂ࠲ࡂ࠲㝼⡺߅ࠃ߮↢ᱺᎽ⢽⢌㉄⚵ᚑߩᄌേ  ⫾㙃ߦࠃࠆ㝼⡺⢽⢌㉄⚵ᚑߩᄌേߪ 10-12 ᦬ߩࡂ࠲ࡂ࠲㝼⡺⢽⢌㉄⚵ᚑ 7)ߣᲧセߔࠆߣዊߐ޿௑ะߢ޽ߞߚ‫↢ߒ߆ߒޕ‬ᱺᎽߩ⢽⢌㉄⚵ᚑᄌേߪߘࠇߣㅒߩᄌേࠍ␜
ߒ‫ޔ‬IPA ߿ DHA ߩࠃ߁ߥᄙଔਇ㘻๺⢽⢌㉄ߩᷫዋ߇㗼⪺ߢ޽ߞߚ‫☻ޕ‬⢽⢌㊂ߩᄌേߦ߅޿ߡ↢๮⛽ᜬ߇ఝవߩᄌേࠍ␜ߒߚ߇‫ޔ‬⢽⢌㉄⚵ᚑߢ߽↢๮⛽ᜬఝవߩᄌേ
߇޽ࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬㧠㧚⫾㙃ࡂ࠲ࡂ࠲㝼⡺߅ࠃ߮↢ᱺᎽߩㆆ㔌ࠕࡒࡁ㉄ߩᄌേ  ⫾㙃㓽ࡂ࠲ࡂ࠲ߩ㝼⡺ߩㆆ㔌ࠕࡒࡁ㉄ߢߪ࠲࠙࡝ࡦ‫ࠕ࡞࡯ࡁ࠲ࠛޔࡦ࡝࠮ࡦࠕޔ‬ࡒࡦ߇ᄙߊᬌ಴ߐࠇߚ‫ߪࡦ࡝࠮ࡦࠕޕ‬㝼㘃ߦ߅޿ߡࡑࠣࡠ‫ࠠࠫࠞޔࠝ࠷ࠞޔ‬㘃╬ߩ⿒り㝼㘃ߩ╭⡺ߦᄙߊ⊕り㝼㘃ߢߪዋߥ޿߇ࠨࠤ㘃ߢߪᄙߊ฽߹ࠇࠆ 8)‫ࠤࠨࡠࠪޕ‬ߦ߅޿ߡߪㆆ㔌ࠕࡒࡁ㉄ߩ 60%એ਄ࠍභ߼‫ޔ‬ᕈᚑᾫߦࠃࠅࡉ࠽ൻ߇ㅴ߻ߣߐࠄߦࠕ
ࡦ࠮࡝ࡦ฽㊂߇Ⴧടߔࠆ 9)‫ߩ╬ࠬ࠙ࡑ߿࠻࠶࡜ߪࡦ࡝࠮ࡦࠕޕ‬േ‛ߢߪᵴᕈ㉄⚛㒰෰╬ߦࠃࠆ᛫㉄ൻ૞↪߿ㆇേ⽶⩄߇ᄙߊ╭⡺ਛߦ੃㉄߇⫾Ⓧߒߚ㓙ߩ✭ⴣലᨐ૞↪ࠍᜬߟߣߐࠇߡ޿ࠆ 10)‫ߪࡦ࡝࠮ࡦࠕޔࠄ߆ߣߎߩࠄࠇߎޕ‬ㆊᐲߩㆇേ‫⛘ޔ‬㘩‫࠲ޔ‬ࡦࡄࠢ⾰ಽ⸃╬ߩࠬ࠻࡟ࠬⅣႺߦ޽ࠆ႐วߦ╭⡺ਛߦวᚑߐࠇ‫⫾ޔ‬㙃ࡂ࠲ࡂ࠲ߦ߅
޿ߡ߽⛘㘩ߥߤߦࠃࠅࠕࡦ࠮࡝ࡦ߇ᄙߊߥߞߚߣផቯߐࠇࠆ‫⫾ޔߚ߹ޕ‬㙃㓽ࡂ࠲ࡂ࠲ෆᎽ߅ࠃ߮♖Ꮍߢߪࠛ࠲ࡁ࡯࡞ࠕࡒࡦ߇ᬌ಴ߐࠇߚ߇‫ࡦ࡝ߪࠇߎޔ‬⢽⾰ߩ৻ߟߢ޽ࠆࡎࠬࡈࠔ࠴ࠫ࡞ࠛ࠲ࡁ࡯࡞ࠕࡒࡦߩ᭴ᚑᚑಽߢ޽ࠆߎߣ߆ࠄ↢ᱺᎽਛߩ࡝ࡦ
⢽⾰ಽ⸃ߣߩ૗ࠄ߆ߩ㑐ㅪ߇ផኤߐࠇࠆ‫ޕ‬એ਄⫾㙃ߦ߅ߌࠆࡂ࠲ࡂ࠲㝼⡺ߣ↢ᱺᎽߩ☻⢽⢌฽㊂‫ޔ‬⢽⢌㉄⚵ᚑ߅ࠃ߮ㆆ㔌ࠕࡒࡁ㉄⚵ᚑߩᄌേࠍ✚ว⊛ߦ⠨߃ࠆߣ‫↢ߡߴߔޔ‬๮⛽ᜬߩߚ߼ߩଏ⛎Ḯߣߒߡߔࠆ
ߚ߼㝼⡺߿↢ᱺᎽߩᚑಽߪಽ⸃‫ޔ‬ᶖ⾌ߐࠇࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬㧡㧚⫾㙃ෆ႙ߩᒻᘒ⊛ᄌൻ 㓽ࡂ࠲ࡂ࠲߆ࠄណขߒߚෆ႙ߪ⫾㙃㐿ᆎ߆ࠄ 2 ㅳ߹ߢߪᒻᘒ⊛ߦᄌൻ߇ߥ޿߇‫ޔ‬
3 ㅳ⋡߆ࠄ☼⾰‛ߩಽ⸃߇⿠ߎࠆߎߣ߇ᒻᘒߣ☼⾰‛㊂ߩᄌൻ߆ࠄផኤߐࠇࠆ‫৻ޕ‬ᣇ‫ޔ‬SDS-PAGE ߦࠃࠆಽᨆߢߪ⫾㙃 4 ㅳߢ߽ᄌൻߪߥߊ‫ޔ‬ቭ⢻⊛ߥࡉ࡝ࠦߩ☼ࠅ߿ෆߩ㘩ᗵ‫ޔ‬㝼⡺ߩ㘩ᗵߪ 4 ㅳߦ߅޿ߡ߽⦟ᅢߢ޽ߞߚߎߣ߆ࠄ‫ߩߎޔ‬Ბ㓏ߩ☼⾰‛ಽ⸃ߪ☼ࠅߩቭ⢻⊛⹏ଔߦߪᓇ㗀ߒߥ޿ߣ⠨߃ࠄࠇࠆ‫ޕ‬㧢㧚⫾㙃ߦࠃࠆຠ⾰଻ᜬߩታ↪ൻ ࡂ࠲ࡂ࠲ෆ႙ߪ⑺↰⋵ߢߪࡉ࡝ࠦߣ⒓ߐࠇ㕖Ᏹߦᅢ߹ࠇߡ޿ࠆ‫࠲ࡂ࠲ࡂޕ‬ෆ႙ߩ☼ࠅ╬ߩ㘩ᗵߪ㓽ࡂ࠲ࡂ࠲ߩຠ⾰ࠍ⹏ଔߔࠆ਄ߢ㊀ⷐߢ޽ࠆ‫ࠍߺߩࠅ☼ߩࠦ࡝ࡉޕ‬ᜰᮡߣߒߡ⹏ଔߒߚ႐วߪ‫ޔ‬4 ㅳ߹ߢ⫾㙃ߦࠃࠆຠ⾰଻ᜬ߇㞲㝼ߣߒߡน⢻ߢ޽ࠆߣ⠨߃ࠄࠇࠆ‫ޔߒ߆ߒޕ‬4 ㅳߢߪ㝼⡺⢽⾰߿㝼⡺࠲ࡦࡄࠢ⾰ߪಽ⸃௑ะߦ޽ࠅ‫↥ޔ‬ෆࠃࠅ↢๮⛽ᜬ߇ఝవߐࠇ↢ᱺᎽᚑಽ߇ಽ⸃ߔࠆߎߣ‫ߩࠦ࡝ࡉޔ‬ᒻᘒ⊛ᄌൻ߇޽ࠆ
ߎߣࠍ✚ว⊛ߦ⠨߃ࠆߣ‫ߩߘޔ‬ຠ⾰ᄌൻ߇௖߆ߢ޽ࠆߎߣ߆ࠄ 2 ㅳ߹ߢߪ⫾㙃ߦࠃࠆຠ⾰଻ᜬ߇㞲㝼ߣߒߡน⢻ߢ޽ࠆߣ⠨߃ࠄࠇࠆ‫ߩߎޔߚ߹ޕ‬ᣇᴺࠍታ↪ൻߔࠆߚ߼ߦߪ੹ᓟ‫ࠍࠬ࡟࠻ࠬޔ‬シᷫߒߚ⫾㙃ᣇᴺࠍᬌ⸛ߒ‫ߦࠄߐޔ‬ຠ⾰ᄌൻ߇ዋߥߊ㐳ᦼ㑆ߩຠ⾰଻ᜬ߇ߢ߈ࠆࠃ߁ᬌ⸛߇ᔅⷐߣ⠨߃ࠄࠇࠆ‫ޕ‬
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‫ޣ‬ᒁ↪ᢥ₂‫ޤ‬1) ┻ౝବ㇢‫ޔ‬ᷰㄝ ᱞ㧚ࠦࠗ߅ࠃ߮࠾ࠫࡑࠬߩ૕⚵ᚑ߅ࠃ߮⢽⢌㉄⚵ᚑߦ෸߷ߔ⛘㘩߅ࠃ߮᳓᷷ߩᓇ㗀㧚ᣣ᳓⹹㧚48: 1307-1316(1982)2) ਃဈዏⴕ‫ޔ‬᳓㊁િ਽‫ޔ‬ች⣶㕏ਯ‫┻ޔ‬ౝൎᏈ‫ޔ‬㣔⷗㆐਽‫═ޔ‬ේ ᣹㧚㘫㙈ਛߩࠨࠢ࡜ࡑࠬᒰᱦ㝼ߩ⢄⤳ߦ߅ߌࠆ࠻࡝ࠣ࡝࠮࡝࠼߅ࠃ߮ࠣ࡝ࠦ࡯ࠥࡦ฽㊂ߩᄌേ㧚ᣣ᳓⹹㧚 70: 168-174(2004)3) ႇ ᔘ৻㧚᳓ಽ㧚‫ޟ‬㘩ຠಽᨆᴺ‫ޠ‬㧔ᣣᧄ㘩ຠᎿᬺቇળ㘩ຠಽᨆ✬㓸ᆔຬળ✬㧕㧔ᩣ㧕శ℘‫᧲ޔ‬੩㧚 61-62(1982)4) ⩲ේ㦖ᐘ‫ޔ‬೨Ꮉᤘ↵㧚⢽⾰㧚‫ޟ‬ᣂ㘩ຠಽᨆࡂࡦ࠼ࡉ࠶ࠢ‫ޠ‬㧔ᩣ㧕ᑪᏡ␠‫᧲ ޔ‬੩㧚59-60(2000)5) ᚭᨑ৻༑‫ޔ‬Ⴆᧄ⎇৻‫ޔ‬㜞ᯅᔀ‫᧖ޔ‬ጊ⑲᮸‫ᧁ⦁ޔ‬ീ㧚‫࠲ࡂ࠲ࡂޟ‬ෆᎽ↱᧪ߩ☼⾰‛‫ޔ‬ߘߩขᓧᣇᴺ߅ࠃ߮↪ㅜ‫ޠ‬㧚․㗿㧞㧜㧜㧟㧙㧟㧝㧡㧝㧠㧞㧚2003.9.8 ಴㗿6) ᅏ᧛ት᣿㧚㔚᳇ᵒേᴺ㧚‫⾰ࠢࡄࡦ࠲ޟ‬ታ㛎ࡁ࡯࠻਄᛽಴ߣಽ㔌♖⵾‫(ޠ‬ጟ↰㓷ੱ‫ޔ‬ችፒ㚅✬)㧔ᩣ㧕⟠࿯␠‫᧲ޔ‬੩㧚14-52(1996)7) Ⴆᧄ⎇৻‫ޔ‬ᚭᨑ৻༑‫ ᧁ⦁ޔ‬ീ‫ޔ‬๺↰⦹⟤ሶ‫␽ᧄ᧻ޔ‬ሶ‫᧻ޔ‬᳗㓉ม㧚⑺↰⋵ᴪጯᶏၞߢṪ₪ߐࠇߚࡂ࠲ࡂ࠲ Arctoscopus japonicus ߩ⡺߅ࠃ߮↢ᱺᎽਛߩ⢽⾰ᚑಽߩቄ▵⊛ᄌേ㧚ᣣ᳓⹹㧚73: 897-904(2007)8) ⮮↰⌀ᄦ㧚⣄ᬁേ‛ߩ฽⓸⚛ൻว‛㧚‫ޟ‬᳓↥ቇࠪ࡝࡯࠭ 72 㝼੺㘃ߩࠛࠠࠬᚑಽ‫ޠ‬㧔ဈญ቞ᒾ✬㧕㧔ᩣ㧕ᕡᤊ␠ෘ↢㑑‫᧲ޔ‬੩㧚25-43(1988)9) ⠀↰㊁౐↵㧚ࡉ࠽ൻߣᚑಽᄌൻ㧚‫ޟ‬᳓↥ቇࠪ࡝࡯࠭ 55 ⑺ࠨࠤߩ⾗Ḯߣ೑↪‫ޠ‬㧔ᐳ㑆ብ৻‫ޔ‬㜞ᯅ⵨຦✬㧕㧔ᩣ㧕ᕡᤊ␠ෘ↢㑑‫᧲ޔ‬੩㧚68-83(1985)10)Ᏹ⍹ᩕ૞㧚ࠕࡦ࠮࡝ࡦ‫ࡦࠪࡁ࡞ࠞޔ‬㧚ᛛⴚ↪⺆⸃⺑㧚ᣣ㘩Ꮏ⹹㧚53: 362-363(2006)− 16 −

大野剛

秋田県総合食品研究センター報告

Vol. 13, 17 − 23（2011）

ᷡ㈬߽ࠈߺ߳ߩ♧ᷝടࠍ೑↪ߒߚᣂⷙ㈬ㅧᛛⴚߩᬌ⸛

ᄢ㊁೰ ઁ
㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧕
6UW[QUJK1*01‫*ޔ‬KVQUJK6#-#*#5*+‫*ޔ‬C[CVQ57)+/161㨮6CMCPQDW6#)7%*+‫ޤ⚂ⷐޣ‬ᐔᚑ  ᐕߩ㈬⒢ᴺᡷᱜߦࠃࠅᷡ㈬߽ࠈߺ߳ᷝടߒߚ♧㘃ߩ⊒㉂߇น⢻ߦߥߞߚߩߢ‫ࠍࠇߎޔ‬೑↪ߒߚᣂⷙ㈬ㅧᛛⴚࠍᬌ⸛ߒߚ‫ޕ‬ᷡ㈬߽ࠈߺ߳ߩ♧ᷝടߩᷡ㈬ᚑಽ
߳ߩᓇ㗀ߦߟ޿ߡᬌ⸛ࠍⴕߞߚ⚿ᨐ‫ޔ‬એਅߩ․ᓽ߇⹺߼ࠄࠇߚ‫ޕ‬
ᷡ㈬߽ࠈߺ߳ߩ♧ᷝട⊒㉂ߦࠃࠅ‫ޔ‬㈶㉄ࠗ࠰ࠕࡒ࡞ߩ↢ᚑߪჇട‫ࠛ㉄ࡦࡠࡊࠞޔ‬࠴࡞ߪᷫዋߔࠆ௑ะߢ޽ߞߚ‫㉄ޕ‬ᐲߪჇടߒߚ߇‫㉄ࡁࡒࠕޔ‬ᐲߪૐਅߒߚ‫ᧃ☳ޕ‬᳓㘸ߦࠃࠆ৻ㇱઍᦧ૶↪ߦࠃࠅ‫㉄ޔ‬ᐲߩჇടߪᛥ߃ࠄࠇߚ‫ีޕ‬㉯㈬ߩࠃ߁ߥ․ᓽࠍ߽
ߞߚ㈬㘃ߩ⵾ㅧߩߚ߼ߦߪ‫ޔ‬㚅ࠅߩ↢ᚑߣ㉄๧ߩࡃ࡜ࡦࠬ߆ࠄ‫࡞࠹ࠬࠛޔ‬㜞↢ᚑ㉂Უࠍ૶↪ߔࠆߎߣ߇᦭ലߢ޽ߞߚ‫ޕ‬એ਄ߩ⍮⷗߆ࠄ‫ޔ‬ᷡ㈬߽ࠈߺ߳ߩ♧ᷝടߦࠃߞ
ߡ‫㉄ߢࠖ࠹࡯࡞ࡈޔ‬๧߇޽ࠅࠕࡒࡁ㉄ᐲߩૐ޿ᷡ㈬ߩ⵾ㅧ߇น⢻ߣ⠨߃ࠄࠇߚ‫ޕ‬‫ޤ⸒✜ޣ‬ ᐔᚑ  ᐕ  ᦬  ᣣ߆ࠄᣉⴕߐࠇߚ㈬⒢ᴺߩᡷᱜߦࠃࠅ‫ߢ߹ࠇߘޔ‬ᷡ㈬⵾ㅧߦ㑐ߔࠆᛚ⹺ၮḰߦࠃߞߡ⺞๧ᶧߣߒߡ߽ࠈߺᧃᦼߦᷝടߔࠆᣇᴺߩߺߦ೙㒢ߐࠇߡ޿ߚ
‫ޟ‬᡽઎ߢቯ߼ࠆ‛ຠߩ߁ߜ‫⽷ޔ♧࠙࠼ࡉޔ‬ോ⋭઎ߢቯ߼ࠆ♧㘃‫ࠍޠ‬ේᢱߣߒߡ‫ޔ‬છᗧߩᤨᦼߦᷝടߒ‫⊒ޔ‬㉂ߐߖࠆߎߣ߇⹺߼ࠄࠇߚ㧔ᩮ᜚ᴺ઎ ᴺ㧟৾ࠗ㧕‫♧࠙࠼ࡉޕ‬ߥߤ♧㘃ߪ‫ޔ‬ਇ⚐‛ࠍ฽᦭ߒߥ޿ߎߣ‫ߦߺࠈ߽ޔ‬ቢోߦṁ⸃ߔࠆߎߣߥߤ‫↱☨⊕ޔ‬᧪ߩ♧⾰ߦߥ޿ᕈ⾰ࠍ᦭ߔࠆ‫ߩߎޔ߼ߚߩߘޕ‬ᡷᱜࠍ೑↪ߒߚ♧㘃ᷝട⊒㉂ߦࠃࠆ
ᷡ㈬⵾ㅧᴺߩᄙ᭽ൻߣ‫ޔ‬ᓥ᧪ߦߥ޿ࠦࠬ࠻ࡄࡈࠜ࡯ࡑࡦࠬߦఝࠇߚᷡ㈬߇⵾ㅧߢ߈
ࠆน⢻ᕈ߇⠨߃ࠄࠇߚ‫ޕ‬♧ൻߣ⊒㉂ࠍಽ㔌ߒߚ㉯ㅧᴺ㧔♧ൻᓟ⊒㉂ᴺ㧕ߩᬌ⸛ߪ⑺↰ࠄ㧝㧙㧟㧕ߦࠃࠅⴕࠊࠇ
ߡ߅ࠅ‫ޔ࠯࡯࡜ࠚࡈࠬࡦ࡜࠻࡞࠴࠮ࠕ࡞࡯ࠦ࡞ࠕޔ‬㤕૶↪㊂ߣ㈶㉄ࠛࠬ࠹࡞‫ޔ‬㜞⚖ࠕ࡞ࠦ࡯࡞↢ᚑߦߟ޿ߡߩႎ๔߇ߐࠇߡ޿ࠆ‫ߚ߹ޕ‬᪀ේࠄ㧠㧕ߪ߽ࠈߺ߳ߩ♧⊒㉂⹜
㛎ࠍⴕߞߡ޿ࠆ߇‫⃻ޔ‬࿷ߩี㉯㚅ߩਥᚑಽߢ޽ࠆࠞࡊࡠࡦ㉄ࠛࠬ࠹࡞ߩ↢ᚑߦߟ޿ߡߪ⸒෸ߐࠇߡ޿ߥ޿‫ޕ‬ᧄ⎇ⓥߢߪᐔᚑ  ᐕߩ࿖⒢ዪߩႎ๔㧡㧕ࠍߪߓ߼ߣߔࠆࠕ࡞ࠦ࡯࡞㘶ᢱߩฦ⒳Ꮢ
႐⺞ᩏߦࠃࠅᓧࠄࠇߚࡑ࡯ࠤ࠶࠻ࠗࡦဳߩࠠ࡯ࡢ࡯࠼ߢ޽ࠆ‫ޟޔޠࠖ࠹࡯࡞ࡈޟ‬シᔟߐ‫ޟޔޠ‬ᓟญߩ⦟ߐ‫ޟޔޠ‬Ყセ⊛቟ଔ‫  ߩޠ‬ὐߩ᧦ઙࠍߺߚߒ‫ีޔ‬㉯㈬ߩࠃ߁ߥ㚅ࠅߩ․ᓽࠍ߽ߞߚ㈬㘃ߩ⵾ㅧࠍ⋡⊛ߣߒߚ‫♧ޕ‬ᷝട⊒㉂ߦࠃࠆ߽ࠈߺ߳ߩᓇ㗀ߦߟ޿ߡᬌ⸛ࠍⴕ޿‫ޔ‬ᣂߚߥᷡ㈬㉯ㅧᛛⴚߣߒߡ♧㘃ᷝട㈬ߩ⵾ㅧᴺߦߟ޿ߡ⍮⷗ࠍᓧࠆߎ
ߣ߇ߢ߈ߚ‫ޕ‬
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‫ޣ‬ታ㛎ᣇᴺ‫ޤ‬㧝㧚 ♧ᷝടᴺߩᬌ⸛㧝㧕ታ㛎᧚ᢱ
㤕☨ߦߪ♖☨ᱠว 㧑⑺↰㈬ߎ߹ߜ‫ޔ‬ដ☨ߦߪ♖☨ᱠว 㧑⑺↰㈬ߎ߹ߜࠍ૶↪
ߒߚ‫ޕ‬㉂Უߪ⑺↰ᵹ⧎㉂Უ #- ࠍ૶↪ߒߚ‫ޕ‬㧞㧕⊒㉂⹜㛎㤕ࠛࠠࠬߦߡ ͠‫ ޔ‬ᣣ㑆ၭ㙃ߒߚ㉂Უၭ㙃ᶧࠍ㈬Უߣߒ‫ޔ‬Ᏹᴺߤ߅ࠅਃᲑ઀ㄟ
ߣߒߚ‫↪૶☨⊕ߪ☨✚ޕ‬㊂ࠍ  ࠣ࡜ࡓߣߒߚ‫ޕ‬ᷝടߔࠆ♧ߪో㊂ࡉ࠼࠙♧ߣߒ‫ޔ‬♧ᷝടᤨᦼߪ‫ޔ‬Ԙဋ╬ᷝട㧦ᦨ㜞ࡏ࡯ࡔ೔㆐ᓟࠃࠅ  ᣣ㑆ဋ╬ߦᷝട‫ޔ‬ԙ৻᜝ᷝട㧦⇐઀ㄟᤨߦ৻᜝ో㊂ᷝടߩ  ♽⛔ߣߒߚ‫⋧ࠎ߲ࠎߢࠍ♧ޕ‬ᒰ㊂឵▚㧔ࡉ࠼࠙♧ 㧩⊕☨ 㧕ߢ⵬ᱜ⸘▚ࠍⴕ޿‫☨⊕ޔ‬឵▚ࠍⴕߞߚ਄ߢ‫ޔ‬㤕ᱠวߪ 㧑߆ࠄ 㧑ߦߥࠆࠃ߁⺞ᢛߒߚ‫ޕ‬㈬㘃ߩᛚ⹺ၮḰߢߪ‫ޔ‬᡽઎ߢቯ߼ࠆ‛ຠߩ㊀㊂߇⊕☨ߦኻߒ 㧑એਅߩᷝടߢ޽ࠇ߫ᷡ㈬‫ߚ߃⿧ࠍࠇߘޔ‬႐ว࡝ࠠࡘ࡯࡞ߣ඙ಽߐࠇࠆߎߣࠍൊ᩺ߒ‫♧ޔ‬ᷝട㊂ߪ⊕☨૶↪㊂ߦኻߒ‫ޔ‬Ԙ㧑‫ޔ‬ԙ㧑‫ޔ‬Ԛ㧑‫ޔ‬ԛ㧑ߣߒ‫ޔ‬ήᷝട඙ࠍኻ⽎ߣߒߚ‫ޕ‬♧ᷝടߪ߽ࠈߺߩේࠛࠠࠬߦ⋧ᒰߔࠆ 㧑㧔㨃㧛㨂㧕᳓ṁᶧࠍᷝടߔࠆᣇᴺߢታᣉ
ߒߚ‫ޕ‬᷷ᐲ⚻ㆊߪี㉯⚻ㆊߣߒ‫ޔ‬਄ᮏᤨᦼߪᣣᧄ㈬ᐲr ࠍ⋡ᮡߣߒߚ‫ޕ‬਄ᮏߪ㆙ᔃಽ㔌ߦߡታᣉߒߚ‫ޕ‬৻⥸ಽᨆߪᚲቯᴺߦᓥ޿‫ޔ‬㚅᳇ᚑಽߪࠟࠬࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ࠍ↪޿ߡࡋ࠶࠼ࠬ
ࡍ࡯ࠬᴺߦߡ᷹ቯߒߚ‫ޕ‬㧢㧕㧞㧚 㤕೑↪ᴺߩᬌ⸛㧝㧕ታ㛎᧚ᢱ㤕ߦߪੇ῎㤕㨀㧔ᓼፉ⵾㤕㧕‫ޔ‬ដ☨ߦߪ♖☨ᱠว 㧑ጊ↰㍪ࠍ૶↪ߒߚ‫ޕ‬㉂Უ
ߪ⑺↰ᵹ⧎㉂Უ #- ࠍ૶↪ߒߚ‫ޕ‬㧞㧕⊒㉂⹜㛎⹜㛎඙ಽࠍ⴫㧝ߦ␜ߔ‫♧ޕ‬ᷝടᣇᴺߪ‫ޔ‬਄⸥ታ㛎ᣇᴺߣห᭽ߦ 㧑㧔㨃㧛㨂㧕᳓ṁᶧࠍᷝടߔࠆᣇᴺߢ‫ޔ‬Ԙဋ╬ᷝട‫ޔ‬ԙ৻᜝ᷝടࠍታᣉߒߚ‫♧࠙࠼ࡉޕ‬ᷝട㊂ߪ⊕☨㊀㊂Ყ 㧑ߣߒߚ‫ޕ‬⴫㧝 㤕೑↪ᴺࠍᬌ⸛ߔࠆ઀ㄟ㈩วߣ♧ᷝടᴺ৻ⷩ
඙ಽ
㪈
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㪊
㪋
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㪎
㪏
ήᷝട

⇛⒓
㪉㪊㪢
㪉㪇㪢
㪈㪎㪢
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㪉㪊㪫
㪉㪇㪫
㪈㪎㪫
㪈㪋㪫
㪉㪇㪥

઀ㄟ㈩ว
⫳☨
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㪉㪎㪇
㪉㪐㪇
㪊㪈㪇
㪉㪌㪌
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㤕☨
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♧
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㪈㪍㪇
㪈㪍㪇
㪇

᳹᳓
㪏㪍㪇
㪏㪍㪇
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㪏㪍㪇
㪏㪍㪇
㪏㪍㪇
㪍㪍㪇

㤕ᱠวኻ♧⾰
䋨䋦䋩
㪉㪊
㪉㪇
㪈㪎
㪈㪋
㪉㪊
㪉㪇
㪈㪎
㪈㪋
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㤕ᱠวኻ⊕☨
䋨䋦䋩
㪊㪍
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㪊㪉
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♧ᷝടᣇᴺ

ᷝടᣣ

ဋ╬
ဋ╬
ဋ╬
ဋ╬
৻᜝
৻᜝
৻᜝
৻᜝
䈭䈚

㪋㪄㪈㪊㪻㪸㫐
㪋㪄㪈㪊㪻㪸㫐
㪋㪄㪈㪊㪻㪸㫐
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⇐ᤨ
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䈭䈚



♧ൻᶧࠍ↪޿ߚන⊒㉂⹜㛎㧝㧕‫ޔ‬㧟㧕ߢ㤕ᱠว 㧑߆ࠄ 㧑߇㜞⹏ଔߢ޽ߞߚߚ߼‫ޔ‬㤕ᱠวߪ♧ࠍߢࠎ߲ࠎ⋧ᒰ㊂឵▚ߢ⵬ᱜ⸘▚ࠍⴕߞߚ਄ߢ‫ޔ‬឵▚ᓟ⊕☨㊂ߦኻߒ‫ޔ‬Ԙ㧑‫ޔ‬ԙ㧑‫ޔ‬Ԛ㧑‫ޔ‬ԛ㧑ߩ  ඙ಽߣߒߚ‫ޕ‬ታ㓙ߩ⊕☨ߦኻߔࠆ㤕ᱠวߪߘ
− 18 −

ࠇߙࠇԘ㧑‫ޔ‬ԙ㧑‫ޔ‬Ԛ㧑‫ޔ‬ԛ㧑ߣߥߞߚ‫ޕ‬ᷡ㈬ߩ↢ᚑߪࠕ࡞ࠦ࡯࡞ᐲ 㧑ࠍ⋡ᮡߣߒߚ‫ޕ‬㧟㧚 㜞㚅᳇↢↥㉂Უߩ૶↪㧝㧕ታ㛎᧚ᢱ
ේᢱ☨ߦߪ㤕☨‫ޔ‬ដ☨ߣ߽ߦ♖☨ᱠว 㧑⑺↰㈬ߎ߹ߜࠍ૶↪ߒߚ‫ޕ‬ᷝട♧㘃ߪ
ో㊂ࡉ࠼࠙♧ߣߒߚ‫ޕ‬㧞㧕⊒㉂⹜㛎⹜㛎඙ಽࠍ⴫㧞ߦ␜ߔ‫ޕ‬㉂Უߦߪ‫ޔ‬Ԙ⑺↰㉂Უ 0Q㧔ᭂ߼ߡ㜞޿㈶㉄ࠗ࠰ࠕࡒ࡞↢ᚑ⢻‫࠽࠽ࡃޔ‬᭽․ᓽ㚅㧕‫ޔ‬ԙ⑺↰㉂Უ 0Q㧔㜞޿ࠞࡊࡠࡦ㉄ࠛ࠴࡞↢ᚑ⢻‫ࡔޔ‬
ࡠࡦ᭽․ᓽ㚅㧕ࠍ૶↪ߒߚ‫ޕ‬㧣㧕♧ᷝട㊂ߣᷝടᣇᴺߪ‫ޔ‬Ԙኻ⊕☨㊀㊂Ყ 㧑⇐઀ㄟᤨ৻᜝ᷝട‫ޔ‬ԙห 㧑⇐઀ㄟᤨ৻᜝ᷝട‫ޔ‬Ԛห 㧑ဋ╬ᷝടߩ  ඙ಽࠍ⸳ቯߒ‫ߘޔ‬ࠇߙࠇήᷝട඙ࠍኻ⽎ߣߒߚ‫ޕ‬㤕ᱠวߪኻ⊕☨㊀㊂Ყߢ 㧑ߣߒߚ‫ޕ‬⴫㧞 㜞㚅᳇↢↥㉂Უߩ૶↪ߦࠃࠆ⊒㉂⹜㛎඙ಽ 
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‫ޣ‬ታ㛎⚿ᨐߣ⠨ኤ‫ޤ‬㧝㧚 ♧ᷝടᴺߩᬌ⸛৻᜝ᷝട඙ߢߪဋ╬ᷝട඙ߣ⇣ߥࠅ‫઀⇐ޔ‬ㄟᓟᢙᣣߦ߅޿ߡ߽Ꮧ⭯߽ߣߩࠃ߁ߦ
࿕ᒻㇱ߇ᶧ㕙߆ࠄ㔺಴ߒߥ߆ߞߚ‫߽ࠇߊ߰ޕ‬㗼⪺ߢߪߥߊ‫ޔ‬ᵹേᕈࠍᜬߞߡ޿ߚ‫ޕ‬ဋ╬ᷝട඙ߢߪ⇐ᓟ  ᣣ⋡ߦᦨ㜞ࡏ࡯ࡔߣߥࠅ‫ޔ‬හᣣ♧ᷝടࠍ㐿ᆎߒߚ‫♧ޕ‬ᷝടߪ㧟㧢㧑᳓ṁᶧߣߒߡታᣉߒߚߚ߼‫♧ޔ‬ᷝടߦᓥ޿ߔߴߡߩᷝട඙ߢᶧㇱ߇Ⴧടߒߚ‫ޕ‬޿ߕࠇߩᷝട඙߽☨☸⥄૕ߩṁߌౕวߪㅢᏱ⒟ᐲߢ޽ߞߚ‫ޕ‬㧑ᷝട඙ಽߦ߅޿ߡ
ߪ㉂Უߩ⥄Ꮖᶖൻ⥇߇ᗵߓࠄࠇߚߚ߼‫ޔ‬ᣣᧄ㈬ᐲ ઃㄭߢߩ਄ᮏߣߥߞߚ‫ޕ‬
⵾ᚑ㈬ߩᚑಽߪ⴫㧟ߦߺࠄࠇࠆࠃ߁ߦ♧ᷝടߦ߅޿ߡ‫㉄ޔ‬ᐲߩ਄᣹ߣࠕࡒࡁ㉄ߩૐਅ߇ߺࠄࠇߚ‫߽ࠇߕ޿ޕ‬ဋ╬ᷝടߩᣇ߇ᄌൻ㊂߇ᄢ߈޿௑ะߢ޽ߞߚ‫ีޕ‬㉯㚅ߩਥᚑಽߢ޽ࠆ㈶㉄ࠗ࠰ࠕࡒ࡞‫↢࡞࠴ࠛ㉄ࡦࡠࡊࠞޔ‬ᚑ㊂ߪ‫ߩߺࠈ߽ޔ‬න⚐Ꮧ㉼ߦࠃࠆ⸘▚୯એ਄ᓧࠄࠇߚᷝട඙߇ᄙߊ‫৻ޔ‬ㇱߩᷝട඙ߢჇട߽⹺߼ࠄࠇߚ‫ޕ‬ဋ╬ᷝടߢߪ 㧑ᷝട඙ߢ‫৻ޔ‬᜝ᷝടߢߪ 㧑߆ࠄ 㧑ᷝട඙ߢี㉯㚅ߩ↢ᚑ㊂߇Ყセ⊛㜞ߊ‫ߩࠇߙࠇߘޔ‬ᷝടᴺߦ߅޿ߡ᦭ലߥᷝട㊂ߣᕁࠊࠇߚ‫ޕ‬᪀ේࠄ㧠㧕ߪ‫☨⊕ޔ‬㊀㊂
Ყ 㧑ߩࡉ࠼࠙♧ߩ㓒ᣣᷝടߢ໧㗴ߥߊ⊒㉂߇ㅴࠎߛߣߒߡ߅ࠅ‫ޔ‬਄⸥ᷝട㊂ߪ߽ࠈߺ▤ℂ਄‫߮ࠃ߅ޔ‬㚅᳇ᚑಽ↢ᚑߦኻߒߡ߽᦭ലߣផኤߐࠇߚ‫㉄ޕ‬ᐲߩჇടߦߟ޿
ߡߪ⸃♧♽ߩჇᒝ‫♧ޔ‬ᷝടߩ㓙ߩ㉄⚛ଏ⛎ߦࠃࠆ㉂Უߩ 6%# ࿁〝ߩჇㅴߥߤⶄᢙߩ
ⷐ࿃߇⠨߃ࠄࠇߚ‫ޕ‬
− 19 −⴫㧟 ♧ᷝട㊂ߣᷝടᤨᦼߩ㆑޿߇㈬⾰ߦਈ߃ࠆᓇ㗀
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㧞㧚 㤕೑↪ᴺߩᬌ⸛৻᜝ᷝട඙ߢߪᦨ㜞ࡏ࡯ࡔ߇  ࠍ⿧߃ߚ߽ߩߩ‫ޔ‬㗅⺞ߦ⊒㉂ߒߚ‫ޕ‬ဋ╬ᷝട඙ߢߪࡏ࡯ࡔ‫ࠬ࡯ࠦ࡞ࠣޔ‬Ớᐲߩᄢ߈ߥᄌൻߪߺࠄࠇߥ߆ߞߚ߇‫ޔߢ߆߿✭ߪ࡟ࠠޔ‬ᣣ⋡ઃㄭߢ৻᜝ᷝട඙ߣㅒォߒ‫ߩߘޔ‬ᓟ߽਄ᮏ߹ߢࡏ࡯ࡔ‫߽ߣࠬ࡯ࠦ࡞ࠣޔ‬ᱷࠅ᳇๧ߢ⚻ㆊߒߚ㧔࿑㧝㧕‫ޕ‬࿑㧝 ♧ᷝട߽ࠈߺߦ߅ߌࠆࡏ࡯ࡔߣࠣ࡞ࠦ࡯ࠬỚᐲߩផ⒖ ㅊ޿᳓ߦࠃࠆᚑಽ⺞ᢛߪో඙ಽߦߟ޿ߡታᣉߖߕ‫࡞࡯ࠦ࡞ࠕޔ‬ᐲ 㧑ࠍ⋡ᮡߣߒߡ㆙ᔃಽ㔌ߦࠃࠅ⵾ᚑߒߚ‫⵾ޕ‬ᚑ㈬ߩᚑಽࠍ⴫㧠ߦ␜ߔ‫ޕ‬
⵾ᚑ㈬ߩේࠛࠠࠬߪ‫ޔ‬㤕ᱠวߩ㜞޿඙ಽ߆ࠄ‫ޔ‬ဋ╬ᷝട඙ߢ  ߆ࠄ ‫৻ޔ‬᜝ᷝട඙ߢ  ߆ࠄ ‫☺ޔ‬ᱠวߪหߓߊဋ╬ᷝട඙ߢ 㧑߆ࠄ 㧑‫৻ޔ‬᜝ᷝട඙ߢ 㧑߆ࠄ 㧑ߣߥߞߚ‫ޕ‬หߓ㤕ᱠวߢߪဋ╬ᷝട඙ߢ☺ᱠว߇ૐߊ‫ޔ‬ේᢱ೑↪₸߇㜞߆ߞߚ‫㉄ޕ‬ᐲߪ‫ޔ‬㤕ᱠว߇㜞޿඙ߢ㜞ߊ‫৻ޔ‬᜝ᷝട඙ߦᲧߴဋ╬ᷝട඙ߢ߿߿㜞޿௑ะ߇⷗ࠄࠇߚ‫㉄ࡁࡒࠕޕ‬ᐲߪ‫ޔ‬㤕ᱠว߇㜞޿඙ߢ㜞ߊ‫ޔ‬ኻᾖߣᲧセߒߡ㤕ᱠวኻ♧⾰㧔㧑㧕឵▚ߢ‫ޔ‬㤕ᱠว߇ᄙ޿ߣჇട‫ޔ‬ዋߥ޿ߣᷫዋߔࠆ௑ะ߇
⷗ࠄࠇߚ‫৻ޕ‬᜝ᷝടߣဋ╬ᷝടߢߩᏅߪ᣿ࠄ߆ߢߪߥ߆ߞߚ‫ޕ‬
− 20 −

⴫㧠 㤕ᱠวߣ♧ᷝട㊂߇♧ᷝട⊒㉂߽ࠈߺߦਈ߃ࠆᓇ㗀
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᦭ᯏ㉄ߦߟ޿ߡߪ‫ߦ⊛ᤨ⚻ߪ㉄ࠧࡦ࡝ޔ‬Ⴧടߒ‫ߦߊߣޔ‬ဋ╬ᷝട඙ߢჇട߇㗼⪺ߢ޽ߞߚ‫ߪ㉄੃ޕ‬ᒰೋ৻᜝ᷝട඙ߢૐ޿߽ߩߩ‫ޔ‬ဋ╬ᷝട඙ߩᷝട߇⚳ੌߔࠆ ᣣ⋡ઃㄭߢห⒟ᐲߣߥߞߚ‫৻ޕ‬᜝ᷝട඙ߢߪ߽ࠈߺೋᦼߦ㈶㉄ߩ↢ᚑ߇ߺࠄࠇߚ㧔࿑㧞㧕‫ޔ߼ߚߩߘޕ‬ㅊട⹜㛎ࠍⴕ޿‫ޔ‬ᷝട♧⾰ߩ৻ㇱࠍ☳ᧃ᳓㘸㧔62&㧕ߦઍᦧߔࠆߣ‫ޔ‬㉄ᐲჇടߣ㈶㉄↢ᚑߩ⒟ᐲߪᛥ߃ࠄࠇࠆߎߣࠍ⏕⹺ߒߡ޿ࠆ‫ޕ‬࿑㧞 ♧ᷝട߽ࠈߺߦ߅ߌࠆ࡝ࡦࠧ㉄Ớᐲߣ㈶㉄Ớᐲߩផ⒖޿ߕࠇߩᷝട඙ߦ߅޿ߡ߽ࡄࡦ࠻࠹ࡦ㉄ߩਇ⿷ᤨߦߢ߿ߔ޿߽ࠈߺߩ⇣⥇‫ࠊ޿ޔ‬ࠁࠆ⎫ൻ⥇ߪ⊒↢ߒߥ߆ߞߚ‫ޕ‬ㅊട⹜㛎ߦ߅޿ߡ‫☨♖ޔ‬ᱠว 㧑ጊ↰㍪ߩ⓭߈ࡂ࠯ဳ㤕ࠍ૶↪ߒߚ႐วߢ߽⇣⥇ߪ⊒↢ߖߕ‫ࡦࡒ࠲ࡆޔ‬㘃ߩଏ⛎Ḯߣߒߡߪචಽߢ޽ࠆ
ߣ⠨߃ࠄࠇߚ‫⵾ޕ‬ᚑ㈬ߪࠣ࡞ࠦ࡯ࠬ߇߿߿ᱷࠅ᳇๧ߣߥߞߚ߇‫㉄ޔ‬ᐲ߇Ⴧടߒߚߎ
ߣ߽޽ࠅ‫ޔ‬ᣂ↞ㄆᐲ㧤㧕 ߩᜰᮡߢߪ߶ߣࠎߤ߇߿߿↞ญ߆ࠄ߿߿ㄆญߩ▸࿐ߣߥࠅ‫ޔ‬↞๧ߣ㉄๧ߩࡃ࡜ࡦࠬߪߣࠇߡ޿ߚ‫ޕ‬ቭ⢻⹜㛎ߢߪ㤕ᱠวዋߥ߼ߩ඙ಽ‫ޔ‬ဋ╬ᷝട඙ߢ㤕ᱠวኻ♧⾰ 㧑‫৻ޔ‬᜝ᷝട඙ߢ 㧑ߢ⵾ᚑߒߚᷡ㈬ߩ✚ว⹏ଔ߇㜞߆ߞߚ‫ޕ‬߹ߚ㤕ᱠว߇㜞޿඙ಽߢᣦ๧࡮㔀๧ߩᜰ៰߇Ⴧടߒߚߎߣ߆ࠄ‫ޔ‬㤕ᱠวߪኻ♧⾰Ყ₸ߢ 㧑บ‫ޔ‬ኻ⊕☨Ყ₸ߢ 㧑บᓟඨ߆ࠄ 㧑บ೨ඨ߇ㆡᒰߣ್ᢿߐࠇߚ‫ߛߚޕ‬ߒ♧ᷝട⊒㉂ߦ߅޿ߡߪ‫ߺࠈ߽ޔ‬න૏㊂޽ߚࠅߩ㉂⚛ജଔߪਅ߇ࠆߩߢ‫⧯ޔ‬ᐓ߽ࠈߺߩߣߌ߇ᖡߊߥࠆ௑ะ߇⹺߼ࠄࠇߚ‫ޕ‬㧟㧚㜞㚅᳇↢↥㉂Უߩ૶↪ ࠕ࡞ࠦ࡯࡞㘶ᢱߩฦ⒳Ꮢ႐⺞ᩏߦࠃࠅᓧࠄࠇߚࠠ࡯ࡢ࡯࠼ߩ৻ߟߢ޽ࠆࡈ࡞࡯࠹
ࠖߩ᧦ઙࠍߺߚߔี㉯㚅ߩ㜞޿㈬㘃ߩ⵾ㅧߩߚ߼ߦߪ‫ޔ‬㜞޿ࠛࠬ࠹࡞↢ᚑ⢻ࠍᜬߟ㉂Უࠍ૶↪ߔࠆߎߣ߇᦭ലߣ⠨߃ࠄࠇߚ‫ߢߎߘޕ‬㜞㚅᳇ᚑಽ↢ᚑ㉂Უߢ޽ࠆ⑺↰㉂− 21 −

Უ 0‫↰⑺ޔ‬㉂Უ 0Q ࠍ↪޿ߚ⵾ㅧᴺߩᬌ⸛ࠍታᣉߒߚ‫ޕ‬
⵾ᚑ㈬ߩᚑಽࠍ⴫㧢ߦ‫ޔ‬ήᷝട඙ࠍ 㧑ߣߒߚ႐วߩᚑಽᲧセࠍ࿑㧟ߦ␜ߒߚ‫ޕ‬⴫㧢 ♧ᷝട⊒㉂߇㜞㚅᳇↢ᚑ㉂Უ߽ࠈߺߦਈ߃ࠆᓇ㗀
඙ಽ

⇛⒓

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䍐䍝䍏䍮䍷䍏䍷䍘䍎䍷
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࿑㧟 ήᷝട඙ࠍ 㧑ߣߒߚ႐วߩฦ⹜㛎඙ߩᚑಽ୯ߩᲧ   ⹜㛎඙ಽߣ⇛⒓ߩ⹦⚦ߦߟ޿ߡߪ ⴫㧞ߦ⸥タ
 #O#E㧦㈶㉄㨼㩉㨻㩚㩣‫'ޔ‬V%CR㩀㩖㩩㩥㩧㉄㨾㩋㩣‫ޔ‬#O1*㧦㨼㩉㨻㩚㩣㨻㩣㩄㨺㩣ߘߩ⚿ᨐ‫㉄ޔ‬ᐲߪ 0 ㉂Უߢኻᾖ 0 ߇  ߢ޽ࠆߩߦኻߒ‫ޔ‬㧑ᷝട඙ ߢ ‫ޔ‬㧑ᷝട඙  ߢ  ߦჇടߒߚ‫㉄ࡁࡒࠕޕ‬ᐲߪኻᾖ 0‫ޔ‬0 ߩ ߦኻߒ‫ޔ‬0 ㉂Უ૶↪඙ߢ  ߆ࠄ ‫ޔ‬0 ㉂Უ૶↪඙ߢ  ߆ࠄ  ߣߥࠅ‫ޔ‬ᷫዋߒߚ‫ޕ‬㚅᳇ᚑಽߦߟ޿ߡߪ㈶㉄ࠗ࠰ࠕࡒ࡞߇ 0 ㉂Უߢኻᾖ 0 ߩ RRO
ߦኻߒ‫ޔ‬㧑ᷝട඙  ߢ RRO‫ޔ‬㧑ᷝട඙  ߢ RRO ߦ‫ޔ‬0Q ㉂Უ
ߢ 0 ߩ RRO ߦኻߒ‫ ߢ ޔ‬RRO‫ ߢ ޔ‬RRO ߣߥࠅ‫ޔ‬Ⴧടߔࠆ௑ะ
ࠍ␜ߒߚ‫ߩ࡞࠴ࠛ㉄ࡦࡠࡊࠞޕ‬Ⴧടߪ⷗ࠄࠇߥ߆ߞߚ‫ޕ‬㧑ᷝട඙ߢߪࠣ࡞ࠦ࡯ࠬ
߇ᱷࠅ‫ޔ‬0 ㉂Უߢߪࠕ࡞ࠦ࡯࡞↢ᚑ߽ㆃࠇߚߎߣ߆ࠄ‫ޔ‬ᷝട㊂ߪ߿߿ㆊ೾ߢ޽
ߞߚน⢻ᕈ߇޽ࠆ‫ޕ‬ᣂ↞ㄆᐲߢߪ‫↞߿߿ޔ‬ญ߆ࠄㄆญߩ⹏ଔߣߥߞߚ‫⚿ޕ‬ᨐߣߒߡ‫ޔ‬0 ㉂Უߪߘߩ․ᓽ㚅ߢ޽ࠆ㈶㉄ࠗ࠰ࠕࡒ࡞߇ߐࠄߦჇടߒ‫ߥ߆߿⪇߁ߘߞ޿ޔ‬㚅ࠅߣߥߞߚ‫ޕ‬0Q ㉂Უߪࠞࡊࡠࡦ㉄ࠛࠬ࠹࡞ߪᷫዋߔࠆ߽ߩߩ‫ޔ‬㈶㉄ࠗ࠰ࠕࡒ࡞ߪჇടߒߚߚ߼‫ޔ‬ㅢᏱ઀ㄟߣ㚅᳇ߩࡃ࡜ࡦࠬ߇⇣ߥࠅ‫․ޔ‬ᓽ⊛ߥ㈬⾰ߣߥߞߚ‫ޕ‬⑺↰ࠄ㧝㧕ߪන⊒㉂♽ߦ߅޿ߡ‫ޔ‬㤕ᱠว 㧑ߢ㈶㉄ࠗ࠰ࠕࡒ࡞߇Ⴧട‫ޔ‬㧑ߢࠞࡊࡠࡦ㉄ࠛ࠴࡞߇ᓸჇߔࠆߣߒߡ޿ࠆ‫⹜ᧄޕ‬㛎ߦ߅޿ߡ߽㈶㉄ࠗ࠰ࠕࡒ࡞ߩჇടߒߚ඙
ߪ‫ޔ‬ኻ⊕☨㤕ᱠวߪ 㧑ߛ߇‫ޔ‬ኻ♧⾰ߢߪ 㧑ᷝട඙ߢ 㧑‫ޔ‬㧑ᷝട඙ߢ 㧑
− 22 −

ߢ޽ࠅ‫ޔ‬㘃ૃߒߚ⚿ᨐߣߥߞߚ‫ޕ‬‫ޤ߼ߣ߹ޣ‬ᷡ㈬߽ࠈߺ߳ߩ♧ᷝട⊒㉂߇㈬⾰ߦ޽ߚ߃ࠆᓇ㗀ࠍᬌ⸛ߒߚ⚿ᨐ‫ߩ♧ޔ‬ᷝടߣ⊒㉂᧦ઙߦߟ޿ߡߩᣂߒ޿⍮⷗߇ᓧࠄࠇ‫ߩߎޔ‬ᛛⴚࠍ↪޿ߚᣂ໡ຠ㐿⊒߇ᦼᓙߐࠇࠆ‫ޕ‬ᒰೋ⸳ቯߒߚᏒ႐⺞ᩏߦࠃࠅᓧࠄࠇߚ  ὐߩࠠ࡯ࡢ࡯࠼ߦߟ޿ߡ‫ޔ‬Ԙ‫࠹࡯࡞ࡈޟ‬ࠖߐ‫ߦޠ‬ኻߒߡߪ‫ีޔ‬㉯㚅ߢ޽ࠆ㈶㉄ࠗ࠰ࠕࡒ࡞ߩჇᒝ‫ޔ‬ԙ‫ޟ‬シᔟߐ‫ߦޠ‬ኻߒߡߪૐࠕࡒࡁ㉄ᐲߦࠃࠆ๧ߩシߐߩታ⃻‫ޔ‬Ԛ‫ޟ‬ᓟญߩ⦟ߐ‫ߦޠ‬ኻߒߡߪ‫㉄ޔ‬๧࡮↞๧ߩࡃ࡜ࡦࠬߩ⺞ᢛ‫ޔ‬ԛ‫ޟ‬Ყセ⊛቟ଔ‫ߦޠ‬ኻߒߡߪ‫♧ޔ‬ᷝടᢙ㊂ߩ⸳ቯߦࠃࠆේᢱࠦࠬ
࠻ૐᷫൻࠍߘࠇߙࠇታ⃻ߢ߈ࠆឭ᩺߇น⢻ߣߥߞߚ‫ޕ‬߭ߣߟ߼ߩឭ᩺ߪԘ㈶㉄ࠗ࠰ࠕࡒ࡞ߦ․ᓽ߇޽ࠅࡈ࡞࡯࠹ࠖߢࠕࡒࡁ㉄ᐲ߇ૐ޿シᔟߥ࠲ࠗࡊߩ㈬⾰ࠍ⋡ᜰߔ߽ߩߢ‫ޔ‬㜞㚅᳇ᚑಽ↢ᚑ㉂Უࠍ૶↪ߒ‫☨⊕ߦᤨ⇐ޔ‬㊀㊂Ყ 㧑ߩ♧ࠍ⋧ᒰ㊂ߩ᳹᳓ߣߣ߽ߦ৻᜝ᷝടߔࠆ‫ߦࠄߐߪࠇߎޕ‬Ԙ㧦㉄๧ࠍ᦭ߔࠆ࠲ࠗࡊ㧔ో㊂ࡉ࠼࠙♧૶↪㧕Ԙ㧦㉄๧߇㜞ߊߥ޿࠲ࠗࡊ㧔ࡉ࠼࠙♧࡮☳ᧃ᳓㘸૬↪㧕ߦಽߌࠄࠇࠆ‫ߩ߼ߟ ޕ‬ឭ᩺ߪԙ㚅ࠅߪ߭߆߃߼ߢࠕࡒࡁ㉄ᐲ߇ૐߊ‫࡝ޔ‬ࡦࠧ㉄ߥߤߩ㉄๧߇޽ࠅ๧߇シᔟߥ࠲ࠗࡊߢ‫♧ޔ‬ṁᶧࠍዋ㊂ߕߟᦨ㜞ࡏ࡯ࡔᤨ߆ࠄ
ㆡቱᷝടߔࠆ‫ޕ‬ᷝട㊂ߪ⊕☨㊀㊂Ყ 㧑߇ㆡ㊂ߢ‫ޔ‬㧑߇਄㒢ߢ޽ࠈ߁‫ߩ߼ߟ ޕ‬ឭ᩺ߣߥࠆԚૐࠦࠬ࠻ߦ․ᓽߩ޽ࠆ࠲ࠗࡊߪ‫☨⊕ޔ‬㊀㊂Ყ 㧑એ਄ߩ♧ᷝടࠍⴕ߁
ᣇᴺߢ޽ࠆ߇‫ޔ‬ᓥ᧪ߩჇ㉯ߣᲧߴ‫㉄ޔ‬ᐲ߇㜞߼ߣߥࠆએᄖߩ․ᓽ߇ߥߊ‫ޔ‬ૐࠦࠬ࠻ൻએᄖߦ♧ᷝട⊒㉂ߩࡔ࡝࠶࠻ߪᗵߓࠄࠇߥ߆ߞߚ‫ޕ‬
ᧄᛛⴚߪ․ߦ‫ޔ‬Ԙ ߦឭ␜ߒߚ‫㉄ߢࠖ࠹࡯࡞ࡈޔ‬๧߇޽ࠅࠕࡒࡁ㉄ᐲߩૐ޿ᷡ㈬ߩ⵾ㅧߦ᦭ലߥᛛⴚߢ޽ࠅ‫ޔ‬ᓥ᧪ߩ⺞๧ᶧߦࠃࠆᓸ⺞ᢛߣഀ᳓ߩ૬↪ߦࠃࠅ‫ߞ޿ޔ‬ߘ߁ࠦࠬ࠻ࡄࡈࠜ࡯ࡑࡦࠬߦఝࠇߚ‫ีޔ‬㉯㚅ࠍ᦭ߔࠆシᔟߥ࠲ࠗࡊߩᷡ㈬⵾ㅧߩน
⢻ᕈ߇⠨߃ࠄࠇࠆ‫ޕ‬‫⻢ޣ‬ㄉ‫ޤ‬
ᷡ㈬ߦ߅ߌࠆ♧㘃ᷝട⊒㉂㉯ㅧᛛⴚ㐿⊒ߦߟ޿ߡ‫ୃ⎇ޔ‬ຬߣߒߡฃߌ౉ࠇ‫ޔ‬ᜰዉߦᒰߚߞߡߊߛߐ޿߹ߒߚ⁛┙ⴕ᡽ᴺੱ㈬㘃✚ว⎇ⓥᚲዊᨋஜవ↢‫ޔ‬ᣣਅ৻ዅవ↢ߥࠄ߮ߦห⎇ⓥቶߦᣇ‫ߦޘ‬ᗵ⻢↳ߒ਄ߍ߹ߔ‫ޕ‬‫ޣ‬ᒁ↪ᢥ₂‫ޤ‬㧝㧕⑺↰ୃ‫⬒ޔ‬የᔀᄦ‫ޔ‬ᄢ႐ବノ‫ޔ‬㉯ද‫ޔ‬㧔㧕‫ޔ‬㧔㧕㧞㧕⑺↰ୃ‫⬒ޔ‬የᔀᄦ‫ޔ‬ᄢ႐ବノ‫ޔ‬ች㊁િਯ‫⊒ޔ‬㉂Ꮏቇ‫ޔ‬㧔㧕‫ޔ‬㧔㧕㧟㧕⑺↰ୃ‫⬒ޔ‬የᔀᄦ‫ޔ‬ች㊁િਯ‫ޔ‬ศᴛ฀‫ޔ‬㉯ද‫ޔ‬㧔㧕‫ޔ‬㧔㧕㧠㧕᪀ේஜᴦ‫ޔ‬ጊᧄᄹ⟤‫ޔ‬ቝᵤብ‫ޔ‬ᢧ⮮ን↵‫ޔ‬㉯ද‫ޔ‬㧔㧕‫ޔ‬㧔㧕㧡㧕࿖⒢ᐡ⺖⒢ㇱ㈬⒢⺖‫ޔ‬ᐔᚑ  ᐕ  ᦬ ‫ޟ‬ᷡ㈬⵾ㅧᬺߩஜోߥ⊒ዷߦะߌߚ⺞ᩏ⎇ⓥ‫ߦ ޠ‬㑐ߔࠆႎ๔ᦠ
㧢㧕ศᴛ฀‫ޔ‬㉯ද‫ޔ‬㧔㧕‫ޔ‬㧔㧕㧣㧕ᷰㄝ⺈ⴡ‫↰ޔ‬ญ㓉ା‫ޔ‬㜞ᯅੳ‫ޔ‬ᄢ㊁೰‫✚⋵↰⑺ޔ‬ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ႎ๔  ภ‫ޔ‬㧔㧕㧤㧕ቝㇺችੳ‫ޔ‬㉯ද‫ޔޔ‬㧔㧕
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金子隆宏

秋田県総合食品研究センター報告

Vol. 13, 24 − 30（2011）᦭⦡☨ࠍේᢱߣߒߚᣂⷙ↞๧⚛᧚ߩ⹜૞㊄ ሶ 㓉 ብ(✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ડ↹▤ℂቶ)Takahiro KANEKO‫ޤ ⚂ ⷐ ޣ‬ ᦭⦡☨ࠍේᢱߣߒߚᣂⷙ↞๧⚛᧚ࠍ⹜૞⵾ㅧߒߚ‫⵾ޕ‬ㅧߪǩൻ‫♧ޔ‬ൻ‫ޔ‬ỹㆊ‫ޔ‬Ớ❗ߩ 4 Ꮏ⒟ߦࠃࠆ◲⚛ߥ߽ߩߢ޽ߞߚ‫ޕ‬ǩൻߪ⫳ߒ‫ߪ޿ࠆ޽ޔ‬㉂⚛⵾೷ߦࠃࠆᶧൻ
෻ᔕ‫ߩࠇߕ޿ߤߥޔ‬ᣇᴺ߽น⢻ߢ޽ߞߚ‫♧ޕ‬ൻ෻ᔕߦߪ㤕ߩ߶߆᭽‫ߥޘ‬㉂⚛೷ߥߤ߇૶↪ߢ߈‫ࠇߙࠇߘޔ‬๧⾰ߩ⇣ߥࠆ↞๧ᢱߣߥߞߚ‫ޕ‬ታ㛎ቶ࡟ࡌ࡞ߢߩዊⷙᮨ⹜૞‫ޔ‬෸߮ࡄࠗࡠ࠶࠻Ꮏ႐ߢߩ⹜૞ߩ߶߆‫⵾ޔ‬ᴺߩ᭎ⷐߦߟ޿ߡ߽⸥タߒߚ‫ޕ‬‫ޤ ⸒ ✜ ޣ‬ ࿖ߦࠃࠆ㘩Ⴎߩኾᄁ೙ᐲ߇ᑄᱛߐࠇ‫ޔ‬㘩Ⴎߩ⵾ㅧ߇⥄↱ൻߐࠇߡએ᧪‫ޔ‬ฦ࿾ߢ․ᓽ޽ࠆႮ߇↢↥ߐࠇ‫ޔ‬࿾ၞ․↥ຠߣߒߡቯ⌕ߒߟߟ޽ࠆ‫ߟߦ♧⍾ޔࠄ߇ߥߒ߆ߒޕ‬޿ߡߪ‫ޔ‬๺ਃ⋆ߥߤ৻ㇱߩ⵾ຠࠍ㒰ߊߣ࿾ၞߩ․ᓽࠍᛂߜ಴ߒߚ໡ຠߪ௖߆ߢ޽ࠆ‫ޕ‬৻ᣇ‫ߪߢ⋵↰⑺ޔ‬ฎઍ☨ߩ߁ߜ⿒☨߿㤥☨ߣ๭߫ࠇࠆ᦭⦡☨ߥߤ߇ᩱၭߐࠇߡ߈
ߚ‫ࠍ⚛⦡ࡦࠕࠪ࠻ࡦࠕߪ☨⦡᦭ߩߎޕ‬ᄙߊ฽߻ߚ߼Ἲ㘵╬ߦࠃߞߡ⟤ߒ޿ࡉ࠼࠙⦡
ࠍ๒ߔࠆ‫✚⋵↰⑺ޕ‬ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ߢߪߎࠇࠄ᦭⦡☨ߩ৻ߟߢ޽ࠆ⚡㤥☨ࠍේᢱߣߒߡ‫߿⺞⦡ޔ‬㚅ࠅߦఝࠇߚ⥄ὼ↞๧⚛᧚ߩ㐿⊒ࠍⴕߞߡ޿ࠆ‫ޕ‬
ᧄⓂߢߪߎߩ⥄ὼ↞๧⚛᧚ߩ߁ߜ‫ޔ‬㤕޽ࠆ޿ߪ㉂⚛⵾೷ߢ♧ൻߒỚ❗ߒߚ♧ൻࠪࡠ࠶ࡊߩ⵾ᴺߩ᭎ⷐࠍㅀߴ‫ߢ޿ߟޔ‬ታ㛎ቶ࡟ࡌ࡞ߢߩዊⷙᮨ⹜૞‫࠶ࡠࠗࡄ߮ࠃ߅ޔ‬࠻Ꮏ႐ߢߩ⹜૞ࠍⴕߞߚ⚿ᨐߦߟ޿ߡ⸥タߒߚ‫ޕ‬‫⵾ޣ‬ᴺߩ᭎ⷐ‫ޤ‬
⵾ㅧߩᎿ⒟ߪ߅߽ߦ‫ޔ‬1)ǩൻ‫ޔ‬2)♧ൻ‫ޔ‬3)ỹㆊ‫ޔ‬4)Ớ❗ߩ 4 Ბ㓏߆ࠄߥࠆ‫ޕ‬1) ǩൻේᢱ☨ࠍ⫳ߔ‫ߪ޿ࠆ޽ޔ‬ේᢱ☨☳ࠍ㉂⚛ߢᶧൻߔࠆߥߤߒߡ‫♧ߩߜߩޔ‬ൻ෻ᔕࠍࠬࡓ࡯࠭ߦߐߖࠆ‫ޕ‬⫳ߔ႐ว‫ߪ☨⦡᦭ޔ‬ᄖ⊹߇ၷߊ‫♧ߪߢ߹߹ߩ☨₵ޔ‬ൻ߇ㅴⴕߒߥ޿ߚ߼‫☨♖ޔ‬ᱠ
ว 80%߹ߢ♖☨ߔࠆߥߤߒߡᄖ⊹ࠍ㒰߈‫☨⊕ޔ‬ㇱߩߺࠍ⫳ߒ਄ߍࠆ‫ߚߓ↢ߢ☨♖ޕ‬ᄖ⊹ࠍ߰ߊ߻♰ㇱಽߪ઀ㄟߺ᳓ߢ ỘߒടᾲᲕ⩶ᓟ‫ޔ‬਄⸥⫳ߒ☨ߣ૬ߖߡ♧ൻ෻ᔕ߳ଏߔࠆ‫઀ޕ‬ㄟߺ᳓ߪ᳓㆏᳓ߥߤߩ㘶↪᳓ࠍ‫ޔ‬ේᢱ☨ߦኻߒ⚂ 2 ୚㊀㊂ࠍ⋡቟ߣߒߡ૶↪ߔࠆ‫ޕ‬ᶧൻ෻ᔕߦࠃࠅǩൻߔࠆ႐วߪ‫ޔ‬ේᢱ☨ࠍᄖ⊹ߏߣ 150㨪200 ࡒࠢࡠࡦ⒟ᐲߦ☳⎈ߒ‫ ⚂ޔ‬2 ୚㊂ߩ઀ㄟߺ᳓ࠍട߃లಽߦ Ộߔࠆ‫ ޕ‬Ộߣหᤨߦᶧൻ㉂⚛ࠍട߃‫ޔ‬৻ቯᤨ㑆ട᷷ߒߡᶧൻࠍቢੌߔࠆ‫ޕ‬ᶧൻ㉂⚛ߩᷝട㊂߅ࠃ߮ട᷷ߩ᷷ᐲ‫ᤨޔ‬㑆ߥߤߪߘࠇߙࠇߩᶧൻ㉂⚛ߦᔕߓߡⴕࠊࠇࠆ߇‫ޔ‬ᓟㅀߩࡄࠗࡠ࠶࠻Ꮏ႐ߢߩ⹜૞ߦ߅޿
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ߡߪ㊀㊂Ყߢේᢱ☨☳ߩ 1/2000 ⒟ᐲߩ㉂⚛⵾೷(ࠦࠢࠥࡦ SD-A‫ޔ‬ᄤ㊁ࠛࡦࠩࠗࡓ
ᩣᑼળ␠㧝))ࠍട߃‫ޔ‬70oC ߢ 1 ᤨ㑆ട᷷ߒᶧൻߒߚ‫ޕ‬ᶧൻቢੌᓟ‫ޔ‬90㨪95oC ߢ 15㨪30 ಽ⒟ᐲߩടᾲಣℂࠍⴕ޿‫ޔ‬ᶧൻ㉂⚛ࠍᄬᵴߐߖ
ࠆ߇‫ޔ‬ᶧൻ㉂⚛߇ߩߜߩ♧ൻ෻ᔕߦᓇ㗀ࠍ෸߷ߐߥ޿႐วߪ‫߽⇛⋭ޔ‬น⢻ߢ޽ࠆ‫ޕ‬2) ♧ൻǩൻᓟ‫♧ޔ‬ൻ㉂⚛ࠍട߃৻ቯᤨ㑆ട᷷ߒߡ♧ൻ෻ᔕࠍⴕ߁‫ޕ‬ᓟㅀߩታ㛎ቶߢߩዊⷙᮨ⹜૞‫࠻࠶ࡠࠗࡄߪ޿ࠆ޽ޔ‬Ꮏ႐ߢߩ⹜૞ߦ߅޿ߡߪ‫ޔ‬ᷡ㈬↪☨㤕ߩઁ‫ޔ‬ᄤ㊁ࠛࡦࠩࠗࡓߩᷡ㈬↪㉂⚛ߢ޽ࠆࠣ࡞ࠢี‫ ࡦࠥࠢࠦޔ‬SD-A‫ޔ‬྾Ბ↪ TG-B ߥߤࠍ♧ൻ㉂⚛ߣߒߡ૶↪ߒߚ‫(ޕ‬SD-A ߪᶧൻ↪㉂⚛ߢ޽ࠆ߇‫♧ޔ‬ൻ↪ߣߒߡ߽૶↪ߒߚ‫ )ޕ‬ᄤ
㊁ࠛࡦࠩࠗࡓߦࠃࠆߣ‫ߪีࠢ࡞ࠣޔ‬ǩ-ࠕࡒ࡜࡯࠯ߣࠣ࡞ࠦࠕࡒ࡜࡯࠯ߩᷙว⵾೷‫ޔ‬
SD-A ߪǩ-ࠕࡒ࡜࡯࠯⵾೷‫ޔ‬TG-B ߪǩ-ࠣ࡞ࠦࠪ࠳࡯࠯ (࠻࡜ࡦࠬࠣ࡞ࠦࠪ࠳࡯࠯)ߦǩ-ࠕࡒ࡜࡯࠯ߣǪ-ࠕࡒ࡜࡯࠯ (߽ߒߊߪࠣ࡞ࠦࠕࡒ࡜࡯࠯) ࠍዋ㊂ട߃ߚ㉂⚛
⵾೷ߣߩߎߣߢ޽ࠆ‫ޔีࠢ࡞ࠣޕ‬SD-A‫ޔ‬TG-B ߪේᢱ☨ߩ 1/1000 ㊀㊂‫☨ޔ‬㤕ߪේᢱ☨ߩ 1/5 ㊀㊂ࠍᷝടߒ‫ޔ‬TG-B ߪ 52oC ߢ‫ ߪઁޔ‬55oC ߢ৻ᄛ (16h) ട᷷ߒߚ‫ޕ‬ߥ߅‫ޔ‬਄⸥ߩᶧൻ߿♧ൻߪ㉂⚛૞↪ߢ޽ࠅ‫ޔ‬෻ᔕߪ pH ߦᓇ㗀ߐࠇࠆ‫ޕ‬ᓟㅀߩ⹜૞ߢߪ‫ߩߡߴߔޔ‬Ꮏ⒟ߦ߅޿ߡ pH ߪᧂ⺞ᢛߩ߹߹ⴕߞߚ߇‫ޔ‬pH ߇ 5.5㨪6.5 ࠍᄢ߈ߊᄖࠇࠆߎߣߪήߊ‫ޔ‬㗅⺞ߦㅴⴕߒߚ‫ઁޕ‬ᣇ‫♧ߌࠊࠅߣޔ‬ൻߩೋᦼߥߤߪ‫ޔ‬⍴ᤨ㑆ߢ޽ߞߡ߽ 50oC ࠍਅ࿁ࠆߣ‫߇⩶㉄੃ޔ‬Ⴧᱺߒ෻ᔕᶧ߇㉄ᕈ஥ߦ௑ߊ႐ว߇޽ࠅ‫ޔ‬ᵈᗧࠍⷐߔࠆ‫ޕ‬3) ỹㆊ♧ൻ⚳ੌᓟ⋥ߜߦỹㆊࠍⴕ߁‫ޕ‬ᢙ࡝࠶࠻࡞એਅߩ♧ൻᶧߢ޽ࠇ߫‫ޔ‬㆙ᔃಣℂᓟ 2
㨪3 ᨎ㊀ߨߩࠨ࡜ࠪߢๆᒁỹㆊߥߤⴕ߁‫ޕ‬ᢙච࡝࠶࠻࡞એ਄ߩ႐วߪ♧ൻᶧࠍࡈࠖ
࡞࠲࡯ࡊ࡟ࠬߥߤߢಣℂߔࠆ‫ࠆߔ↪૶ޕ‬ỹᏓߪ৻⥸ᨐ᳝↪‫⥸৻ޔ‬ᷡ㈬↪ߥߤߢలಽ
ߢ޽ࠆ‫(ޕ‬ᔅⷐߥࠄ⃯߫⮺࿯ߥߤỹㆊഥ೷ࠍࡏ࠺ࠖ࡯ࡈࠖ࡯࠼ߔࠆ‫ )ޕ‬ේᢱ߇⚡㤥☨ߥࠄ߫⿒ࡢࠗࡦ᭽ߩࠢ࡝ࠕߥ♧ൻỹᶧ߇ᓧࠄࠇࠆ‫ޕ‬ ỹㆊᓟ‫ޔ‬ỹᶧߩടᾲಣℂ(90㨪95oC ߢ 15㨪30 ಽ⒟ᐲ‫ ߪߊߒ߽ޔ‬105oC ߢ 5 ಽ⒟ᐲ)ࠍⴕ߁‫ޕ‬ỹㆊ೨ߦടᾲߔࠆߣᱷᷲ↱᧪ߩ㔀๧ࠍ↢ߓࠆߚ߼‫ޔ‬ᔅߕỹㆊߒ‫ޔ‬ᱷᷲࠍ㒰޿ߡ߆ࠄടᾲಣℂࠍⴕ߁ߩ߇ᦸ߹ߒ޿‫ޕ‬4) Ớ❗
ᓧࠄࠇߚỹᶧߩᓸ↢‛ᳪᨴࠍㆱߌ଻ሽᕈࠍ਄ߍࠆߚ߼‫ޔ‬ᷫ࿶Ớ❗ߒߡ Bx75 એ਄ߩࠪࡠ࠶ࡊ⁁ߦߔࠆ‫ޕ‬ᓟㅀߩዊⷙᮨ⹜૞ߦ߅޿ߡߪࡠ࡯࠲࡝࡯ࠛࡃࡐ࡟࡯࠲࡯ߢ
65oC ߹ߢട᷷ߒߚ߇‫ߪ⥇⇣ޔ‬ᗵߓࠄࠇߥ߆ߞߚ‫ઁޕ‬ᣇ‫ޔ‬㊍ߢἺ߈਄ߍࠆߥߤߩᏱ࿶
ടᾲỚ❗ߪ⹜ߺߡ޿ߥ޿߇‫ޔ‬႐วߦࠃߞߡߪὶߍ⥇ߥߤ⇣⥇ࠍ↢ߓࠆ߽ߩߣᕁࠊࠇ
ࠆ‫ޕ‬ᢙච࡝࠶࠻࡞એ਄ߩ♧ൻỹᶧߩỚ❗ߢߪ޿ࠊࠁࠆੑ㊀ല↪➧߿㆙ᔃ⭯⤑Ớ❗ེߥߤߩ᳓㘸↪ߩỚ❗ⵝ⟎߇ᦸ߹ࠇࠆ‫ޕ‬
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ේᢱ☨㧔⚡㤥☨ߥߤ㧕  ωǩൻ㧔⫳ߒ‫ޔ‬ᶧൻ෻ᔕ‫ߤߥޔ‬㧕  ω♧ൻ㧔☨㤕‫ޔ‬㉂⚛⵾೷‫ߤߥޔ‬㧕  ωỹㆊ㧔ๆᒁỹㆊ‫߆߶ޔࠬ࡟ࡊ࡯࠲࡞ࠖࡈޔ‬㧕  ωỚ❗㧔ࡠ࡯࠲࡝࡯ࠛࡃࡐ࡟࡯࠲࡯‫ޔ‬᳓㘸↪ߩỚ❗ེ‫߆߶ޔ‬㧕  ω   ↞๧⚛᧚࿑㧝 ⵾ㅧᎿ⒟ߩ᭎ⷐ
‫ޣ‬ዊⷙᮨ⹜૞‫ޤ‬
̆1 ࡝࠶࠻࡞ኈࡆ࡯ࠞ࡯઀ㄟߺ̆
ේᢱߩ᦭⦡☨ߪ⑺↰⋵ᮮᚻᏒᐔ㣮↸↥ߩ⚡㤥☨‫(ޠߜ߹ߎ⚡ޟ‬JA ⑺↰߰ࠆߐߣᐔ
㣮༡ㄘ࠮ࡦ࠲࡯㧞))ࠍ૶↪ߒߚ‫⋵↰⑺ߪ౉⾼ޕ‬⡯ຬᶖ⾌↢ᵴදห⚵ว㧟)ࠍ⚻↱ߒߚ‫ޕ‬ߎߩ⚡ߎ߹ߜ 300g ࠍ♖☨ߒ⊕☨ 238.8g ߅ࠃ߮♰ 60.6g ࠍᓧߚ(♖☨ᱠว 80%)‫ޕ‬ᓧࠄࠇߚ⊕☨ 238.8g ࠍ৻ᄛ᳓㆏᳓ߦᶐẃߒߚߩߜ‫ ߢ☜⫳ޔ‬50 ಽ⫳ߔߎߣߢ‫⫳ޔ‬ߒ☨ 330g ߣߥߞߚ‫৻ޕ‬ᣇ‫ ♰ޔ‬60.6g ߦ᳓㆏᳓ 600g ട߃‫ߡ޿↪ࠍࡉ࡯࡟ࠢ࠻࡯ࠝޔ‬105oC ߢ 5 ಽ㑆ടᾲߔࠆߎߣߢᲕ⩶ಣℂߒߚ‫ޕ‬ߎߩടᾲಣℂߐࠇߚ♰ Ộᶧߦ਄⸥⫳ߒ☨ 330g ࠍട߃‫ޔ‬లಽߦ᡼಄ߒ‫ޔ‬ຠ᷷߇⚂ 55oC ߥ޿ߒ 60oC ߦߥߞߚᤨὐߢ‫ޔ‬ᒰ࠮ࡦ࠲࡯ߢ⵾ㅧߒߚᷡ㈬↪☨㤕 60g ࠍട߃‫ޔ‬
55oC ߢ⚂ 16 ᤨ㑆ട᷷ߒ♧ൻߒߚ‫ޕ‬⚡㤥☨ 300gω    ω⊕☨ 238.8g  ♰ 60.6g   ᳓㆏᳓ 600g
   ω    ω       ω⫳ߔω        ω⫳ߒ☨ 330g    105oC 5min ಣℂ   ω        ω        ω̪࿐ߺߪේᢱ‫࡯࠳ࡦࠕޔ‬        ωφ☨㤕 60g
࡜ࠗࡦߪਛ㑆↢ᚑ‛        ω
55oC ߢ 16h ♧ൻ෻ᔕ࿑㧞 ዊⷙᮨ⹜૞ߢߩ઀ㄟߺ
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♧ൻ෻ᔕᓟ‫ޔ‬㆙ᔃಣℂ(10,000rpm‫ޔ‬15min‫ޔ‬25oC)ߒߚᧄ♧ൻᶧࠍ‫ࡠ࡯࠽ࡈࡉޔ‬࡯࠻ࠍ↪޿ߡੑᨎ㊀ߨߩࠨ࡜ࠪߢๆᒁỹㆊߒߡਇṁᕈ↹ಽࠍ㒰߈‫ࡦࠗࡢ⿒ޔ‬᭽ߩ⿒⦡♧ൻỹᶧ(Bx27)ࠍ⚂ 730ml ࿁෼ߒߚ‫ޕ‬ᓧࠄࠇߚỹᶧ⚂ 730ml ࠍࡠ࡯࠲࡝࡯ࠛࡃ
ࡐ࡟࡯࠲࡯ߢ Bx75 ߹ߢỚ❗ߒ‫☨⦡᦭ޔ‬Ớ❗♧ᶧ 236.5g ࠍᓧߚ‫ޕ‬ߥ߅‫ޔ‬એ਄ߩᎿ⒟ߢ‫☨ޔ‬㤕ߦઍࠊࠅ‫ޔีࠢ࡞ࠣޔ‬TG-B‫ޔ‬SD-A ߥߤࠍ↪޿ߡߩ♧ൻ߽ⴕߞߚ‫ޕ‬૗ࠇߩ♧ൻ㉂⚛߽⚡ߎ߹ߜ 300g ߦኻߒ 0.3g ᷝടߒߚ‫♧ߚ߹ޕ‬ൻ᷷ᐲߪࠣ࡞ࠢี‫ޔ‬SD-A ߪ 55oC ߢ‫ޔ‬TG-B ߪ 52oC ߢⴕߞߚ‫☨ޕ‬㤕ߢ♧ൻߒߚ߽ߩߪᒝ
޿↞ߺߣ㤕㚅߇޽ࠅࡈ࡞࡯࠹ࠖߢ޽ߞߚ߇‫♧ߢีࠢ࡞ࠣޔ‬ൻߒߚ߽ߩ߽☨㤕ߩ߽ߩߣ㆖⦡ߥ޿↞ߺࠍᗵߓߚ‫ߦࠄࠇߎޕ‬Ყߴ SD-A ߦࠃࠆ߽ߩߪⓏ߿߆ߥ↞ߺߢ޽ࠅ‫ޔ‬
TG-B ߦࠃࠆ߽ߩߪ↞ߺࠍᛥ߃ߚࠦࠢߩ޽ࠆ߽ߩߢ޽ߞߚ‫⚵♧ߩࠇߙࠇߘޕ‬ᚑࠍᣣᧄ࠳ࠗࠝࡀࠢࠬᩣᑼળ␠ߩ HPAEC ࠪࠬ࠹ࡓߦࠃࠅಽᨆߒߚ‫ ࡓ࡜ࠞ(ޕ‬CarboPac
PA-1(4 x 25mm)‫ޔ‬ᬌ಴ེ ED-40‫ޔ‬ṁ㔌ᶧߪ 0.5M ㈶㉄࠽࠻࡝࠙ࡓߣ 0.1M ⧦ᕈ࠰࡯࠳ߩࠣ࡜ࠫࠚࡦ࠻‫ޔ‬ᵹㅦ 1.0ml/min‫☨ )ޕ‬㤕޽ࠆ޿ߪࠣ࡞ࠢีߦࠃࠆ߽ߩߪ޿ߕࠇ
߽ࠣ࡞ࠦ࡯ࠬ (G1) ߩߺᬌ಴ߐࠇ‫( ࠬ࡯࠻࡞ࡑޔ‬G2) એ਄ߩࠝ࡝ࠧ♧㘃 (Gn) ߪᬌ಴ߐࠇߥ߆ߞߚ‫ޕ‬SD-A ࠍ♧ൻ㉂⚛ߣߒߡ↪޿ߚ႐วߪ G1:G2:Gn ߩᲧߪ 5:3:2 ߢ޽ߞߚ‫ޕ‬TG-B ߩ႐วߪࡑ࡞࠻ࠝ࡝ࠧ♧ߩઁߦࠗ࠰ࡑ࡞࠻࡯ࠬ㧔IG2㧕߿ਃ♧એ਄ߩࠗ࠰ࡑ ࡞ ࠻ ࠝ ࡝ ࠧ ♧ (IGn) ߥ ߤ ߩ ↢ ᚑ ߽ ߺ ࠄ ࠇ ‫ ޔ‬G1:G2:Gn:IG2:IGn ߩ Ყ ₸ ߪ ߅ ࠃ ߘ
4:1:1:2:2 ߢ޽ߞߚ‫♧ߩࠇߙࠇߘޕ‬ൻ೷ߦࠃࠆỚ❗♧ᶧߩ♧⚵ᚑߣ๧⾰ࠍ⴫㧝ߦ␜ߔ‫ޕ‬⴫㧝 ฦ⒳♧ൻ೷ߦࠃࠆ⚡㤥☨ߩ♧ൻ♧ൻ೷  ᷝട㊂   ♧ൻ᷷ᐲ ↢ᚑ♧⚵ᚑ    ๧ߩශ⽎              )))P+)+)P☨㤕      Q%  ) ߩߺ↢ᚑ  ᒝ޿↞ߺߣ㤕㚅ࠣ࡞ࠢี   ‫     ޖ     ޖ‬ᒝ޿↞ߺ
5&#    ‫  ޖ   ޖ‬㧡㧟㧞㧜㧜 Ⓩ߿߆ߥ↞ߺ6)$    ‫   ޖ‬Q%  㧠㧝㧝㧞㧞 ࠦࠢߩ޽ࠆ↞ߺ1) ේᢱ☨ߦኻߔࠆ㊀㊂Ყ2) ಽᨆᣇᴺ෸߮⋭⇛⸥ภ(G1,G2 ߥߤ)ߪᧄᢥෳᾖ
߹ߚ‫ߦߜ߹ߎ⚡ޔ‬ઍࠊߞߡ⑺↰⋵ㄘᨋ᳓↥ᛛⴚ࠮ࡦ࠲࡯ㄘᬺ⹜㛎႐ࠃࠅឭଏߐࠇ
ߚ⚡㤥☨‫ޟ‬ዊ⚡‫ࠍޠ‬ේᢱ☨ߣߒߡ૶↪ߒߡ߽ห᭽ߦỚ❗♧ᶧ߇⵾ㅧน⢻ߢ޽ߞߚ‫ޕ‬‫࠻࠶ࡠࠗࡄޣ‬Ꮏ႐ߢߩ⹜૞‫ޤ‬̆ࠫࡖࠤ࠶࠻ઃ 200 ࡝࠶࠻࡞ኈ࠲ࡦࠢ઀ㄟߺ̆
♧ൻේᢱߪ⑺↰⋵↥⚡㤥☨‫ࠍޠ⚡ᦺޟ‬ᐔဋ☸ᓘ 150 ࡒࠢࡠࡦ⒟ᐲߦ☳⎈ߒߚ߽ߩࠍ૶↪ߒߚ‫ߩ⚡ᦺޕ‬ᚻ㈩෸߮⵾☳ߪ(ᩣ)᷆〝⵾☳㧠)ߦଐ㗬ߒߚ‫ޕ‬
− 27 −

ߎߩ☳⎈ߐࠇߚᦺ⚡⚂ 59kg ߦ᳓㆏᳓ 106kg ෸߮ᶧൻ㉂⚛ SD-A30g ࠍട߃‫ޔ‬ᠣᜈߒߥ߇ࠄቶ᷷ߢ 15 ಽ‫ ߢ޿ߟޔ‬70oC ߢ㧝ᤨ㑆ട᷷ߒ‫ޔ‬ේᢱ☨ߩố☳⾰ࠍǩൻߒߚ‫ޕ‬ߎߩᓟ‫ޔ‬ຠ᷷ࠍ 90oC ߣߒߚ߹߹ 15 ಽ㑆଻ߟߎߣߢᶧൻ㉂⚛ࠍᄬᵴߐߖᶧൻ෻ᔕࠍ
ቢੌߒߚ‫ޕ‬ߎߩᶧൻᶧࠍ 52oC ߹ߢ಄ළߒ‫ޔ‬TG-B ࠍ 60g ട߃‫ޔ‬52oC ߢ⚂ 16 ᤨ㑆ട᷷ߒ‫ޔ‬♧ൻ෻ᔕࠍⴕߞߚ(⴫㧞)‫ޕ‬
⴫ 2 ♧ൻᶧ㈩ว㧔TG-B㧕    ේᢱ☨☳    MI᳓㆏᳓    
ᶧൻ㉂⚛ 5&#    
♧ൻ㉂⚛ 6)$    ว⸘        MI     ෻ᔕᓟ‫♧ޔ‬ൻᶧࠍ 157.35kg ࿁෼ߒߚ‫઀ޕ‬ㄟߺ⚳ੌᤨߩว⸘㊀㊂ߪ 165.09kg ߢ޽ߞߚߩߢ‫♧ޔ‬ൻᓟߩ࿁෼ᠲ૞ߦࠃࠆ៊ᄬߪ 7.74kg ߢ޽ߞߚ‫ޕ‬ߎߩ♧ൻ෻ᔕᶧࠍࡈࠖ࡞࠲࡯ࡊ࡟ࠬߢ࿶៦ಣℂߒਇṁᕈ↹ಽࠍ㒰޿ߚ‫ޕ‬ᓧࠄࠇߚ࿶៦ỹㆊᶧߪ 97.1kg‫ޔ‬Bx32.0 ߢ⿒ࡢࠗࡦ᭽ߩ⿒⦡♧ൻᶧߢ޽ߞߚ‫ઁޕ‬ᣇ‫ޔ‬࿶៦☺
ߩ㊀㊂ߪ 52.0kg ߢ޽ࠅ‫ޔ‬࿶៦ỹㆊߦࠃࠆ៊ᄬߪ 8.25kg ߢ޽ߞߚ‫ޕ‬㧔ߜߥߺߦ‫ޔ‬࿶៦☺ߪṯߒ㙕⁁ߩ㤥⚡⦡ߢలಽߥ↞ߺ߇޽ߞߚ߇ᓟ๧ߦࠛࠣ๧߇ᗵߓࠄࠇߚ‫ޕ‬㧕ߎߩ࿶៦ỹㆊᶧࠍ Bx65 ߦ߹ߢỚ❗ߒ‫☨⦡᦭ޔ‬Ớ❗♧ᶧ 40.8kg ࠍ࿁෼ߒߚ‫ߩߎޕ‬Ớ❗ߦࠃࠆ៊ᄬߪ Bx65 ߩ♧ᶧߦ឵▚ߒߡ 7.0kg ߦ⋧ᒰߒߚ(⴫㧟)‫ޕ‬
⴫ 3 ࿶៦ỹㆊ߅ࠃ߮Ớ❗ᠲ૞ߢߩ↢ᚑ‛࿁෼(TG-B)   ㊀㊂ ៊ᄬ $TKZ   ♧ൻ⚳ੌᤨ   MI   ̆   
࿁෼♧ൻᶧ         
࿶៦ᓟᶧ㊂         
࿶៦☺㊀㊂       ̆    ̆࿁෼Ớ❗ᶧ   MI        ߹ߚ‫ޔ‬એ਄ߩᎿ⒟ߢ‫ޔ‬TG-B ߦઍࠊࠅ‫ޔ‬ᷡ㈬↪☨㤕 12kg ࠍ↪޿ߡߩ♧ൻ߽ⴕߞߚ‫ޕ‬♧ൻ᷷ᐲߪ 55oC ߢ޽ߞߚ‫ޕ‬㧔⴫ 4 ߅ࠃ߮ 5㧕− 28 −

⴫ 4 ♧ൻᶧ㈩ว(☨㤕)    ේᢱ☨☳    MI᳓㆏᳓    
ᶧൻ㉂⚛ 5&#    
♧ൻ↪☨㤕      
㧔ว⸘        MI㧕    

⴫ 5 ࿶៦ỹㆊ߅ࠃ߮Ớ❗ᠲ૞ߢߩ↢ᚑ‛࿁෼(☨㤕)
㊀㊂ ៊ᄬ $TKZ   ♧ൻ⚳ੌᤨ   MI   ̆   
࿁෼♧ൻᶧ         
࿶៦ᓟᶧ㊂         
࿶៦☺㊀㊂       ̆    ̆࿁෼Ớ❗ᶧ   MI         ࡄࠗࡠ࠶࠻Ꮏ႐ߢߩ⹜૞ߦ߅޿ߡ߽ Bx75 એ਄ߩỚ❗ࠍ⋡ᜰߒߚ߇‫੹ޔ‬࿁૶↪ߒߚỚ❗ེߢߪ Bx65 એ਄ߩỚ❗ߪ಴᧪ߥ߆ߞߚ‫੹ޔߚ߹ޕ‬࿁૶↪ߒߚࡄࠗࡠ࠶࠻Ꮏ
႐ߢߪ♧ൻ࠲ࡦࠢ߆ࠄࡈࠖ࡞࠲࡯ࡊ࡟ࠬ‫ޔ‬ᰴ޿ߢỚ❗ེ߳ߣࡄࠗࡊ࡜ࠗࡦ߇ߟߥ߇
ߞߡ߅ࠄߕ‫ޔ‬Ꮏ⒟ߏߣߦ෻ᔕᶧࠍᄢဳࡐ࡝࠲ࡦࠢߢ⒖ㅍߒߚߚ߼‫ޔ‬ฦᎿ⒟ߢߩ៊ᄬ
߇ᄢ߈ߊߥߞߡߒ߹ߞߚ‫ޕ‬‫ ⻢ ޣ‬ㄉ ‫ޤ‬ ᧄ⎇ⓥߪᢥㇱ⑼ቇ⋭ߩ‫ޟ‬ㇺᏒࠛ࡝ࠕ↥ቇቭㅪ៤ଦㅴ੐ᬺ(ᐔᚑ 19㨪21 ᐕᐲ)‫৻ߩޠ‬Ⅳߣߒߡⴕࠊࠇߚ‫⎇ᧄޕ‬ⓥࠍㆀⴕߔࠆߦ޽ߚࠅ‫ޔ‬ዊ═ේඳା਄Ꮸ⎇ⓥຬ‫ޔ‬㜞ᯅੳ਄Ꮸ⎇ⓥຬ‫ޔ‬ᷰㄝ⺈ⴡਥછ⎇ⓥຬߪߓ߼‫ޔ‬ᄙߊߩᣇ‫⾆ߦޘ‬㊀ߥߏഥ⸒ࠍ㗂޿ߚߎߣෘ
ߊ߅␞↳ߒ਄ߍ߹ߔ‫ޕ‬‫ޣ‬ෳ⠨⾗ᢱ‫ޤ‬ ̆ේᢱ☨߅ࠃ߮㉂⚛⵾೷ߩ౉ᚻవ̆
㧝㧕ᄤ㊁ࠛࡦࠩࠗࡓᩣᑼળ␠ ‫ޥ‬460-8630 ᗲ⍮⋵ฬฎደᏒਛ඙㍪ 1-2-7
TEL:052-211-3032 http://www.amano-enzyme.co.jp/jp/index.html
− 29 −㧞㧕JA ⑺↰߰ࠆߐߣᐔ㣮༡ㄘ࠮ࡦ࠲࡯ ‫ޥ‬013-0105 ⑺↰⋵ᮮᚻᏒᐔ㣮↸ᵻ⥰ሼ⑔↰ 158-1 TEL:0182-24-3110 http://www.jafurusato.net/
㧟㧕⑺↰⋵⡯ຬᶖ⾌↢ᵴදห⚵ว 010-0951 ⑺↰⋵⑺↰Ꮢጊ₺ 4-1-1
TEL:018-860-3579 http://akita.kenren-coop.jp/㧠㧕ᩣᑼળ␠᷆〝⵾☳ ‫ޥ‬018-1402 ⑺↰⋵ẟ਄Ꮢᤘ๺ੂᯅሼ㐿਄㑐↰ 122
TEL:018-877-3270 http://www.awajiseifun.co.jp/− 30 −

㧞㧚ේ⪺⺰ᢥ㧔⎇ⓥࡁ࡯࠻㧕Ԙ ‫ޟ‬ടᾲೋᦼߦ☨☸߆ࠄ㔌⣕ߔࠆᚑಽߣ࠺ࡦࡊࡦࠍᷝടߒߡἺ㘵ߒߚ☨㘵ߩ․ᓽ‫ ̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ ޠ‬31ᄢ⢻ବਭԙ ‫⋵↰⑺ޟ‬᷷ᴰ᳓ࠍ↪޿ߚ๧ཬዊ઀ㄟߺ⹜㛎‫ ̖̖̖̖̖̖̖̖̖̖̖̖̖̖ޠ‬35ᷰㄝ㓉ᐘ‫ޔ‬૒⮮ᕺ㉿


大能俊久

秋田県総合食品研究センター報告

Vol. 13, 31 − 34（2011）ടᾲೋᦼߦ☨☸߆ࠄ⣕㔌ߔࠆᚑಽߣ
࠺ࡦࡊࡦࠍᷝടߒߡἺ㘵ߒߚ☨㘵ߩ․ᓽ
ᄢ⢻ବਭ㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧕
Toshihisa OHNO‫ޤ⸒  ✜ޣ‬☨㘵ߩ߅޿ߒߐߦߪ‫☨ޔ‬㘵ߩ⎬ߐ߿☼ࠅߥߤߩ࠹ࠢࠬ࠴ࡖ࡯߇ᄢ߈ߊ㑐ਈߒߡ޿ࠆ
ߣߐࠇ 1)‫☨ޔ‬㘵࠹ࠢࠬ࠴ࡖ࡯ߩේ࿃ࠍ⸃᣿ߔࠆߎߣߪ☨㘵ߩᡷ⦟߿ᡷ⾰ߦᄢ޿ߦᓎ┙ߟ‫ޕ‬ฎ☨ߩ☨㘵࠹ࠢࠬ࠴ࡖ࡯ࠍ SS ⚿วㆶర೷ߢᡷ⦟ߔࠆ႐ว‫ޔ‬ടᾲೋᦼߦ☨☸߆ࠄ⣕㔌ߔࠆ࿕ᒻಽ߇Ⴧടߒ‫☨ߦ⊛⚳ᦨޔ‬㘵ߩエൻ߇⿠ߎࠆߎߣ߇ಽ߆ߞߡ޿ࠆ 2)‫ߩߎޕ‬ߎߣ߆ࠄ‫ޔ‬ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽ߇☨㘵ߩエൻߥߤߩ࠹ࠢࠬ࠴ࡖ࡯ᄌൻߦ㑐ਈߒߡ޿ࠆน⢻ᕈ߇޽ࠆ‫ޔߢߎߘޕ‬ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽߦߟ޿ߡߘߩᚑಽࠍ⺞ߴߚ‫ޕ‬߹ߚ‫ޔ‬ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽߩჇട߇☨㘵࠹ࠢࠬ࠴ࡖ࡯╬߳ਈ߃ࠆᓇ㗀ߦߟ޿ߡ߽ᬌ⸛ߒߚ‫ޕ‬‫ޣ‬ታ㛎ᣇᴺ‫ޤ‬㧝㧚ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽߩᚑಽߩ᷹ቯ ޽߈ߚߎ߹ߜߩ⊕☨ࠍࠕ࡞ࡒࡄ࠙࠴ਛߢ 30͠5 ࡩ᦬⾂⬿ߒߡฎ☨ൻߐߖߚ☨ࠍ૶↪
ߒߚ‫ ☨⊕ߦࡊ࠶ࠞࠬ࡟ࡦ࠹ࠬޕ‬5g ߣ⫳⇐᳓ 8mL ࠍട߃‫ޔ‬1 ᤨ㑆ᶐẃߒߚ‫ޕ‬ᰴߦࠬ࠹ࡦ࡟ࠬࠞ࠶ࡊߏߣ 80͠ߩḡᮏߦ⒖ߒ‫߈߆ޘᤨޔ‬ᷙߗߥ߇ࠄ 5 ಽ㑆ടᾲߒߚ‫ߩߘޕ‬ᓟ‫ޔ‬ࠬ࠹ࡦ࡟ࠬࠞ࠶ࡊࠍḡᮏ߆ࠄขࠅ಴ߒߡ‫⇐⫳ޔ‬᳓ࠍ 10mL ട߃ߡ✭߿߆ߦᠣᜈߒ☨☸
ࠍ㒰߈‫ࠍߩ߽ߚߒ῎ੇޔ‬ടᾲೋᦼߦ☨☸߆ࠄ⣕㔌ߔࠆ࿕ᒻಽߣߒߚ‫ޕ‬ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽߩ☻࠲ࡦࡄࠢ⾰㊂ߪࠤ࡞࠳࡯࡞ᴺ‫☻ޔ‬⢽⢌ߪ㉄ಽ⸃ᴺ‫ޔ‬Ἧಽߪ 550͠⋥ធἯൻᴺߢ᷹ቯߒߚ‫ޕ‬ࠣ࡞ࠦ࡯ࠬ‫ࠬ࡯࠻࡞ࡑޔ♧࡚ࠪޔࠬ࡯࠻ࠢ࡞ࡈޔ‬㊂ߩ᷹ቯߪએਅߩᣇᴺߢⴕߞߚ‫ޕ‬߹ߕ‫ޔ‬ടᾲೋᦼߩ⣕㔌ಽߦ⚳Ớᐲ߇ 80㧑ࠛ࠲ࡁ࡯࡞ߣߥࠆࠃ߁ߦࠛ࠲ࡁ࡯࡞ࠍᷝടߒ‫ޔ‬㆙ᔃߒߡ਄ᷡࠍណขߒߚ‫ߩߘޕ‬਄ᷡࠍᷫ࿶ਅߢੇ῎ߒ⫳⇐᳓ߦṁ߆ߒߚ‫ߩߎޕ‬ṁᶧࠍ
0.45Pm ߩࡈࠖ࡞࠲࡯ߦㅢߒ‫♧ޔ‬ಽᨆࠪࠬ࠹ࡓ DX-500㧔࠳ࠗࠝࡀࠢࠬ␠⵾㧕ࠍ↪޿ߡ㜞ᕈ⢻㒶ࠗࠝࡦ੤឵ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯㧔HPAEC㧕ߢಽᨆߒߚ‫ޕ‬ᬌ಴ེߪ Pulsed
amperometric detector ED40㧔࠳ࠗࠝࡀࠢࠬ␠⵾㧕ࠍ↪޿ߚ‫࡯࠻ࠢ࡞ࡈޔࠬ࡯ࠦ࡞ࠣޕ‬ࠬ‫ߩࠇߙࠇߘޔࠬ࡯࠻࡞ࡑޔ♧࡚ࠪޔ‬Ꮢ⽼⹜⮎ࠍ↪޿ߡᬌ㊂✢ࠍ૞ᚑߒߡ⹜ᢱߩㆆ㔌♧㊂ࠍ᳞߼ߚ‫ޕ‬㧞㧚࠺ࡦࡊࡦࠍᷝടߒߚ☨㘵ߩⷰኤߣ☨㘵࠹ࠢࠬ࠴ࡖ࡯ߩ᷹ቯ

− 31 −

SS ⚿วㆶర೷ߢടᾲߒߚ႐ว‫ޔ‬ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽ߇ኻᾖߦᲧߴߡ☨ 100g
ᒰߚࠅ 0.8㨪1.2g Ⴧടߔࠆ 2)‫ߦ☸☨ࠍࡦࡊࡦ࠺☨ޔߢߎߘޕ‬ኻߒߡ 1㧑ᷝടߔࠆ඙ࠍ⸳
ቯߒߚ‫ࡦࡊࡦ࠺ޔߚ߹ޕ‬ᷝടߩലᨐࠍ᣿⏕ߦᛠីߔࠆߚ߼‫ޔ‬ήᷝട඙ߣ☨࠺ࡦࡊࡦ 3㧑ᷝട඙߽⸳ቯߒߚ‫ޕ‬࠺ࡦࡊࡦࠍᷝടߒߚ☨㘵ߩ࠹ࠢࠬ࠴ࡖ࡯᷹ቯᣇᴺࠍ☨࠺ࡦࡊࡦ 1㧑ᷝട඙ࠍ଀ߦߒ
ߡએਅߦ␜ߔ‫ߦࡊ࠶ࠞࡦ࡝ࡊߩ⵾ࠬ࡟ࡦ࠹ࠬޕ‬਄⸥⊕☨ 9.9g ߣ☨࠺ࡦࡊࡦ㧔ፉ↰ൻቇ‫ޔ‬ࡌ࠲࡯ࡈ࡟ࡦ࠼㧕0.1g ࠍណࠅ‫ޔ‬16g ߩ⫳⇐᳓ࠍട߃ߡࠕ࡞ࡒࡎࠗ࡞ߢ⬄ࠍߒߚ‫ޕ‬1 ᤨ㑆ᶐẃߒ‫ޔ‬㔚᳇Ἲ㘵ེ㧔᧻ਅ㔚ེ⵾ SR-W100㧕ߩ㊍ౝߦ⫳⇐᳓ 75mL ࠍ౉ࠇߡࡊ࡝ࡦ
ࠞ࠶ࡊߏߣ⚂ 12 ಽἺ㘵ߒߚ‫ޕ‬30 ಽ⫳ࠄߒߚ☨㘵ࠍࠪࡖ࡯࡟ߦ⒖ߒߡ⚂ 90 ಽ㕒⟎ߒߚ‫ߩߘޕ‬ᓟ࠹ࡦࠪࡊ࡟࠶ࠨ࡯㧔࠲ࠤ࠻ࡕ㔚ᯏ⵾ TTP-50BXΤဳ㧕ࠍ૶↪ߒߡ☨㘵࠹ࠢࠬ࠴ࡖ࡯ߩ᷹ቯࠍⴕߞߚ‫☨ޕ‬㘵☸ 1 ☸ߕߟߦߟ޿ߡ‫ޔ‬రߩ㜞ߐߩ 10㧑߹ߢ 2mm/s ߩㅦᐲߢ࿶❗ࠍⴕ޿‫ᦨޔ‬࿶❗ὐߢ 0.1 ⑽ߩ஗ᱛᤨ㑆ࠍขࠅ‫ߩߘޔ‬ᓟ 2mm/s ߩㅦᐲߢᒁᒛ
ࠅࠍⴕߞߚ‫ ߪ࡞࠮࠼࡯ࡠޕ‬10kgf‫ࡖࠫࡦ࡜ࡊޔ⵾ࡒ࡞ࠕߪࠫ࡯࠹ࠬߣ࡯ࡖࠫࡦ࡜ࡊޔ‬࡯ߪ⋥ᓘ 25mm ߩ߽ߩࠍ૶↪ߒ‫ޔ‬᷹ቯߪ 25͠ߢⴕߞߚ‫ޕ‬࿶❗⩄㊀ߩᦨᄢ୯ࠍ⎬ߐ‫ޔ‬ᒁᒛࠅ⩄㊀ߩᦨᄢ୯ࠍ☼ࠅߣߒ‫ߢߐ⎬ࠍࠅ☼ޔ‬ഀߞߚ୯ࠍࡃ࡜ࡦࠬᐲߣߒߚ‫ޕ‬☨㘵ߩⷰኤ߽਄⸥ߩᣇᴺߢ⺞⵾ߒߚ☨㘵ߢⴕߞߚ‫ޕ‬㧟㧚᦭ᗧᏅߩᬌቯ ฦ᷹ቯ୯㑆ߩ᦭ᗧᏅߩᬌቯߪ‫৻ޔ‬ర㈩⟎ಽᢔಽᨆ㧔p < 0.05㧕ߦࠃࠅⴕߞߚ‫ޕ‬‫⚿ޣ‬ᨐߣ⠨ኤ‫ޤ‬㧝㧚ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽߩᚑಽ ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽߩੇ‛㊀㊂ߪ☨ 100g ᒰߚࠅ 2.9g ߢ޽ߞߚ‫ޕ‬ടᾲೋᦼߦ
⣕㔌ߔࠆ࿕ᒻಽߩฦᚑಽߩᲧ₸ࠍ⴫ 1 ߦ␜ߔ‫ޔ♧࡚ࠪޔࠬ࡯࠻ࠢ࡞ࡈޔࠬ࡯ࠦ࡞ࠣޕ‬ࡑ࡞࠻࡯ࠬߥߤㆆ㔌♧㊂߇ 0.5㨪2.3㧑ߣዋߥ߼ߢ޽ߞߚߩߦኻߒ‫☻ޔ⾰ࠢࡄࡦ࠲☻ޔ‬⢽⢌‫ޔ‬Ἧಽߪ 3.6㨪6.0㧑ߣㆆ㔌♧ࠃࠅᄙ߆ߞߚ‫ޕ‬਄⸥ᚑಽએᄖߢ☨߆ࠄ⣕㔌ߔࠆ߽ߩߪਥߣߒߡ࠺ࡦࡊࡦߣ⠨߃ࠄࠇࠆߎߣ‫ߚߒ῎ੇޔ‬⣕㔌࿕ᒻಽ߇⤑⁁ߣߥࠅࠝࡉ࡜࡯࠻
ߣหߓᕈ⁁ࠍ␜ߔߎߣ߆ࠄ‫ޔ‬਄⸥ᚑಽߩᱷࠅ 80.5㧑ࠍ࠺ࡦࡊࡦߣ್ᢿߒߚ‫⧎ޕ‬ၔࠄߪ
85͠ߢടᾲಣℂߒߚ㓙ߦࠦࠪࡅࠞ࡝ߩ☨☸߆ࠄ⣕㔌ߔࠆ࿕ᒻಽߩ 90㧑એ਄߇࠺ࡦࡊ
⴫䋱䇭ടᾲᤨ⣕㔌࿕ᒻಽ䈱ᚑಽ㩿ੇ‛឵▚୯䋩฽㊂䋨䋦䋩☻䉺䊮䊌䉪⾰
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         ᷹ቯ୯䈲㪉࿁᷹ቯ䈱ᐔဋ୯䈪␜䈚䈢䋮− 32 −

ࡦߢ޽ࠆߎߣࠍႎ๔ߒߡ޿ࠆ 3)‫੹ޕ‬࿁ߩ⚿ᨐߪ‫ߩߎޔ‬ႎ๔ߣ߶߷৻⥌ߔࠆ߽ߩߢ޽ߞߚ‫ޕ‬ SS ⚿วㆶర೷ߢടᾲߒߚ႐ว‫ࠄ߆☸☨ޔ‬ടᾲೋᦼߦ⣕㔌ߔࠆ࿕ᒻಽ߇Ⴧ߃ߡ☨㘵߇エൻߔࠆ 2)‫ޕ‬⣕㔌ߔࠆ࿕ᒻಽߪਥߣߒߡ࠺ࡦࡊࡦߥߩߢ‫ޔ‬SS ⚿วㆶర೷ߢടᾲߒߚ
႐วߦჇ߃ࠆ⣕㔌ಽ߽࠺ࡦࡊࡦ߇ਥߢ޽ࠈ߁ߣ⠨߃ߚ‫ߩߎޕ‬⣕㔌ߒߚ࠺ࡦࡊࡦ߇Ἲ㘵ᶧਛߦჇടߒ‫☨߼ߚߩߘޔ‬㘵࠹ࠢࠬ࠴ࡖ࡯߇ᄌൻߒߡ޿ࠆน⢻ᕈ߇޽ࠆ‫ޔߢߎߘޕ‬⣕㔌ߔࠆ࿕ᒻಽߦઍ߃ߡ☨࠺ࡦࡊࡦࠍᷝടߒߡ☨㘵ࠍ⺞⵾ߒߡἺ㘵ᶧਛߦ࠺ࡦࡊࡦ߇
Ⴧ߃ࠆᓇ㗀ࠍᰴ㗄ߢ⺞ߴߚ‫ޕ‬㧞㧚࠺ࡦࡊࡦࠍᷝടߒߚ☨㘵ߩⷰኤߣ☨㘵࠹ࠢࠬ࠴ࡖ࡯ ☨࠺ࡦࡊࡦࠍ 3㧑ᷝടߒߚ☨㘵ߩ᭽ሶࠍ࿑ 1 ߦ␜ߔ‫ ࡦࡊࡦ࠺☨ޕ‬3㧑ᷝട☨㘵ߪኻᾖ☨㘵ߦᲧߴߡశᴛ߇Ⴧടߒߡ޿ߚ‫ߩࡦࡊࡦ࠺☨ޔࠄ߆ߣߎߩߎޕ‬ᷝടߪ☨㘵ߩశᴛࠍჇടߐߖࠆലᨐ߇޽ࠆߣ್ᢿߒߚ‫ޕ‬Ἲ㘵ਛߦ☨☸߆ࠄ⣕㔌ߒߚ࠺ࡦࡊࡦߥߤ߇☨㘵⴫㕙ߩ߅ߨ߫ጀᒻᚑߦ㑐ਈߔࠆߎߣ߇ႎ๔ߐࠇߡ޿ࠆ 4,5)‫ޕ‬ᷝടߒߚ☨࠺ࡦࡊࡦ߽‫☨ޔ‬㘵⴫㕙ߦ߅ߨ߫ጀࠍᒻᚑߒߡశ
ᴛ߇Ⴧടߒߚߣ⠨߃ࠄࠇࠆ‫ߩߘޕ‬ᯏᐨߪ‫ޔ‬ᷝടߒߚ☨࠺ࡦࡊࡦ߇ᄙ㊂ߩἺ㘵᳓ߩਛߢ♟ൻߒ‫ޔ‬Ἲ㘵ਛߦỚ❗ߐࠇߡࡍ࡯ࠬ࠻⁁ߦߥࠅ‫☨ߦ⊛⚳ᦨޔ‬㘵⴫㕙ߦઃ⌕ߔࠆߎߣ߇
ਥߢ޽ࠈ߁‫ޕ‬☨࠺ࡦࡊࡦࠍᷝടߒߡἺ㘵ߒߚ☨㘵ߩ࠹ࠢࠬ࠴ࡖ࡯ࠍ⴫ 2 ߦ␜ߔ‫ࡃޔࠅ☼ޔߐ⎬ޕ‬࡜ࡦࠬᐲߩ޿ߕࠇ߽‫ߦ☨ޔ‬ኻߒߡ࠺ࡦࡊࡦࠍ 0‫ޔ‬1‫ޔ‬3㧑ᷝടߒߚ⹜㛎඙㑆ߦ᦭ᗧߥᏅ
ߪ⹺߼ࠄࠇߥ߆ߞߚ‫ ࠍࡦࡊࡦ࠺☨ޕ‬3㧑ᷝടߒߡ߽☨㘵࠹ࠢࠬ࠴ࡖ࡯ߪᄌൻߒߥ޿ߎ
ߣ߇ಽ߆ߞߚ‫ޔࠄ߆ߣߎߩߎޕ‬SS ⚿วㆶర೷ߢ☨㘵߇エൻߔࠆߩߪ‫ޔ‬ടᾲೋᦼߦἺ
㘵ᶧਛߩ࠺ࡦࡊࡦ߇ߚߛනߦჇ߃ࠆߚ߼ߢߪߥ޿ߣ್ᢿߒߚ‫ޕ‬SS ⚿วㆶర೷ߢടᾲ
ߔࠆ႐วߪ‫ޔ‬නߦἺ㘵ᶧਛߦ࠺ࡦࡊࡦ߇Ⴧ߃ࠆߛߌߢߥߊ‫߇ࡦࡊࡦ࠺ࠄ߆☸☨ޔ‬⣕㔌ߒߡߘߩ⚿ᨐἺ㘵ᶧਛߦ࠺ࡦࡊࡦ߇Ⴧ߃ࠆߪߕߢ޽ࠆ‫ࠄ߆☸☨߇ࡦࡊࡦ࠺ޕ‬⣕㔌ߔࠆߎߣ⥄૕߇☨㘵ߩエൻߦ㑐ਈߒߡ޿ࠆน⢻ᕈ߇޽ࠆ‫ޕ‬߹ߚ‫ޔ‬Okadome ࠄߪ߽ߜ☨☨㘵ߩ☼ࠅࠍ࠹ࡦࠪࡊ࡟࠶ࠨ࡯ߣ࠹ࠢࠬ࠴ࡘࡠࡔ࡯࠲࡯
ߢ᷹ቯߒߡᲧセߒ‫☨☨ߜ߽ߪߢ࡯࠲࡯ࡔࡠࡘ࠴ࠬࠢ࠹ޔ‬㘵ߩ☼ࠅ߇ᄢ߈ߊ‫ࡊࠪࡦ࠹ޔ‬− 33 −

⴫㪉䇭䊂䊮䊒䊮䉕ᷝട䈚䈢႐ว䈱☨㘵䊁䉪䉴䉼䊞䊷䊂䊮䊒䊮ᷝട㊂
䋨䋦䋩

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ᐔဋ୯㫧ᮡḰ஍Ꮕ ᐔဋ୯㫧ᮡḰ஍Ꮕ ᐔဋ୯㫧ᮡḰ஍Ꮕ㪇㪊㪊㪅㪏㫧㪋㪅㪍
㪐㪅㪈㪐㫧㪇㪅㪌㪌㪇㪅㪉㪎㪎㫧㪇㪅㪇㪋㪉㪈㪊㪉㪅㪉㫧㪋㪅㪌
㪐㪅㪈㪏㫧㪇㪅㪌㪏㪇㪅㪉㪐㪈㫧㪇㪅㪇㪋㪍㪊㪊㪊㪅㪇㫧㪊㪅㪌
㪐㪅㪊㪌㫧㪇㪅㪍㪍㪇㪅㪉㪏㪎㫧㪇㪅㪇㪊㪐☨㘵㪊㪇☸䉕᷹ቯ䈚䈢䋮  ಽᢔಽᨆ䉕ⴕ䈦䈢䈏䋬䈇䈝䉏䉅᦭ᗧᏅ䋨㫇㩷㪓㩷㪇㪅㪇㪌䋩䈲⹺䉄䉌䉏䈭䈎䈦䈢䋮࡟࠶ࠨ࡯ߢߪᄢ߈ߊߥ޿ߎߣࠍႎ๔ߒߡ޿ࠆ 6)‫ࠬࠢ࠹ߩߤߥ࡯࠲࡯ࡔࡠࡘ࠴ࠬࠢ࠹ޕ‬࠴ࡖ࡯᷹ቯᯏࠍ↪޿ࠇ߫‫ࠍࡦࡊࡦ࠺☨ޔ‬ᷝടߒߡἺ㘵ߒߚ႐วߩ☨㘵࠹ࠢࠬ࠴ࡖ࡯ߩ
ᄌൻࠍ᷹ቯߢ߈ࠆน⢻ᕈ߽޽ࠆ‫ޕ‬‫ޣ‬ᒁ↪ᢥ₂‫ޤ‬1) ⼱㆐㓶㧘ศᎹ⺈ᰴ㧘┻↢ᣂᴦ㇢㧘ၳౝਭᒎ㧘㆙⮮ൟ㧘ᩉἑ⡸㧘ᩕ㙃ߣ㘩♳㧘
22, 452-461(1969).
2) Ohno, T., Tomatsu, M., Toeda, K. and Ohisa, N. Biosci. Biotechnol. Biochem.,
71, 2912-2920 (2007). 3) ⧎ၔൟ㧘ᄥ↰ஜ੺㧘┻↰ජ㊀ਫ㧘᳓਄ᶈਯ㧘┻↰㕏ผ㧘J. Appl. Glycosci., 51,
349-354 (2004).
4) ᳰ↰߭ࠈ㧘ኅ᡽⹹㧘52㧘401-409 (2001).5) ᅏ↰⃍ሶ㧘⍹᧛ືઍ㧘㊄⼱ᤘሶ㧘ᣣ⺞⑼⹹ 42, 394-403 (2009).
6) Okadome, H., Toyoshima, H. and Ohtsubo, K., Cereal Chem., 76, 855-860(1999).

− 34 −

渡辺隆之

秋田県総合食品研究センター報告

Vol. 13, 35 − 38（2011）⑺↰⋵ᄢṚ᷷ᴰ᳓ࠍ↪޿ߚ๧ཬዊ઀ㄟߺ⹜㛎ᷰㄝ㓉ᐘ ઁ㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧕
Takayuki WATANABE㧘Eri SATO‫ޤ⚂ⷐޣ‬ ⑺↰⋵ౝߩᄢṚ᷷ᴰߩ᷷ᴰ᳓ࠍ⒳᳓ߣߒߡ↪޿ߚ๧ཬዊ઀ㄟߺ⹜㛎ࠍታᣉߒߚ‫ޕ‬ᄢṚ᷷ᴰ᳓૶↪๧ཬߪ᳓㆏᳓ࠍ૶↪ߒߚኻᾖ๧ཬߣᲧセߒߡࠣ࡞ࠦ࡯ࠬ‫ޔ‬ฦ⒳ࠕࡒࡁ㉄㊂╬ߩᚑಽ୯ߦᄢ߈ߥᏅ⇣ߪ⹺߼ࠄࠇߥ߆ߞߚ‫ޔߚ߹ޕ‬ቭ⢻ᬌᩏߩ⚿ᨐ‫ޔ⦡ޔ‬㚅ࠅ‫ޔ‬๧‫⚵ޔ‬ᚑߩฦ㗄⋡ߦ߅޿ߡ߽㆖⦡ߪߥߊ‫✚ޔ‬ว⊛ߥ⹏ଔ߽ኻᾖߣห╬ߩ⦟ᅢߥ⚿ᨐ߇ᓧࠄࠇߚ‫ޕ‬‫ޤ⸒✜ޣ‬๧ཬߪ᳓ಽࠍ㧠㧜㨪㧡㧜㧑⒟ᐲ฽᦭ߔࠆ⊒㉂㘩ຠߢ޽ࠆ‫ޕ‬ᷡ㈬‫㉟ޔ‬ᴤ╬ߩઁߩ㉯ㅧ㘩ຠߣห᭽ߦ๧ཬߦ↪޿ࠄࠇࠆ᳓ߩࡒࡀ࡜࡞ಽߥߤߪ⊒㉂ߣຠ⾰ߦᄢ߈ߥᓇ㗀ࠍਈ߃ࠆ࿃ሶߢ޽ࠆߣ⠨߃ࠄࠇࠆ‫৻ޕ‬ᣇ‫ޔ‬᷷ᴰ᳓ߪ↥಴࿾ߦࠃࠅᚑಽ߇ᄢ߈ߊ⇣ߥࠅ‫ޔ‬㘶↪ߩนุߪෘ↢ഭ௛⋭ߩቯ߼ࠆ㘩ຠⴡ↢ᴺߩⷙᩰၮḰߦၮߠ߈‫ޔ‬ㇺ㆏ᐭ⋵ߩ଻ஜᚲߦ
ࠃࠅ್ቯߐࠇߡ޿ࠆ‫ޕ‬ㄭᐕ‫ޔ‬᷷ᴰ᳓㘶ᢱߩ⽼ᄁ߇ో࿖ฦ࿾ߩ᷷ᴰ࿾߆ࠄⴕࠊࠇߡ޿ࠆ
߇‫ޔ‬㘩ຠ᷷߳ᴰ᳓ࠍᷝട‫⎇ߚߒ↪૶ޔ‬ⓥ੐଀ߪዋߥߊ‫ޔ‬๧ཬ⵾ㅧߦ㑐ߒߡߪ⷗޽ߚࠄ
ߥ޿‫ޕ‬ᚒ‫੹ߪޘ‬࿁‫ޔ‬᷷ᴰ᳓ߩ๧ཬߩຠ⾰߳ߩᓇ㗀ࠍ⺞ߴࠆߎߣࠍ⋡⊛ߦ‫ޔ‬㘶↪น⢻ߥ᷷ᴰ᳓ࠍ⒳᳓ߣߒߡ઀ㄟߺᤨߦ૶↪ߒߚዊ઀ㄟߺ⹜㛎ࠍታᣉߒߚߩߢႎ๔ߔࠆ‫ޕ‬‫ޣ‬ታ㛎ᣇᴺ‫ޤ‬ ๧ཬ⵾ㅧዊ઀ㄟߺ⹜㛎ߦ⑺↰⋵ᄢ㙚࿾඙ߩᄢṚ᷷ᴰߩ᷷ᴰ᳓ࠍ⒳᳓ߣߒߡ↪޿ߚ‫ޕ‬ᐔᚑ㧞㧝ᐕ㧢᦬ߦታᣉߒߚಽᨆᯏ㑐ߩಽᨆߦࠃࠆߣߎߩ᷷ᴰ᳓㧝MI ޽ߚࠅߩࡒࡀ࡜
࡞ಽߪ㓁ࠗࠝࡦߢߪ࠽࠻࡝࠙ࡓ OI‫ ࡓ࠙ࠪ࡞ࠞޔ‬OI‫ ࡓ࠙࡝ࠞޔ‬OI‫ࡦࡠ࠻ࠬޔ‬࠴࠙ࡓ OI‫ ࡦࠝࠗࡓ࠙࠾ࡕࡦࠕޔ‬OI‫ ࡓ࠙ࠪࡀࠣࡑޔ‬OI‫ ࡓ࠙࠴࡝ޔ‬OI ߢ޽ࠅ‫ޔࡦࠟࡦࡑޔࡓ࠙࠾ࡒ࡞ࠕޔࡓ࠙࡝ࡃޔ‬㋕㧔Τ㧕‫ޔ‬㋕㧔Υ㧕‫ޔ‬㌃‫ޔ‬੝㋦ߪᬌ಴ߐࠇߡ
޿ߥ޿‫ ߚ߹ޕ‬MI ޽ߚࠅߩ㒶ࠗࠝࡦߣߒߡߪ⎫㉄ OI‫ޔ‬Ⴎ㉄ OI‫ޔ‬὇㉄᳓⚛ OI‫ޔ‬ࡈ࠶⚛ OI‫ ⚛⥇ޔ‬OI‫⎫ޔ‬ൻ᳓⚛ OI‫ ㉄⎫ࠝ࠴ޔ‬OI ߇฽߹ࠇߡ޿ࠆߣߩ⚿ᨐ߇ᓧࠄࠇߡ޿ࠆ‫ޕ‬ᐔᚑ㧞㧞ᐕ㧞᦬ߩ᳓⾰⺞ᩏߢߪᄢ⣺⩶⟲㒶ᕈߢ޽ࠅ৻⥸⚦⩶ߪ
EHWON‫᦭ోޔ‬ᯏ὇⚛ߪ OIN ߣၮḰ୯ࠍਅ࿁ߞߡ޿ࠆ‫੹ޕ‬࿁ߩ⹜㛎ߢߪߎߩ᷷ᴰ
᳓߹ߚߪ᳓㆏᳓‫ޔ‬㉂Უᷝടߩ᦭ήߦࠃࠅએਅߩ㧠඙ಽࠍ⸳ቯߒߚ‫߅ߥޕ‬㉂Უߪᒰ࠮ࡦ
࠲࡯଻ሽᩣߩ㧭㧹㧝ࠍ↪޿઀ㄟߺᤨߩ⩶ᢙ߇㧝㧜 ߦߥࠆࠃ߁ߦᷝടࠍⴕߞߚ‫ޕ‬ − 35 − ඙ಽ㧝  ⒳᳓㧦᳓㆏᳓‫ ޔ‬㉂Უᷝടߥߒ ඙ಽ㧞  ⒳᳓㧦᷷ᴰ᳓‫ ޔ‬㉂Უᷝടߥߒ ඙ಽ㧟  ⒳᳓㧦᳓㆏᳓‫ ޔ‬㉂Უᷝട޽ࠅ ඙ಽ㧠  ⒳᳓㧦᷷ᴰ᳓‫ ޔ‬㉂Უᷝട޽ࠅ
 ઀ㄟߺ㈩วߪ㤕ᱠว㧝㧜ᱠ‫ޔ‬㘩Ⴎಽ㧝㧝㧚㧣㧑‫઀✚ޔ‬ㄟߺ㊀㊂㧟㧚㧤㧡㨗㨓ߢታᣉߒߚ‫ޕ‬඙ಽᲤߩ૶↪ේᢱ㊀㊂ߪಣℂᄢ⼺㧞㨗㨓‫ޔ‬㤕㧝㧚㧞㧡㨗㨓‫ޔ‬㘩Ⴎ㧜㧚㧠㧡㨗㨓‫ޔ‬⒳᳓㧜㧚㧝㧡㨗㨓ߢ޽ࠆ‫੹ߜࠊߥߔޕ‬࿁ߩ᷷ᴰ᳓ᷝട๧ཬߩ᷷ᴰ᳓૶↪ഀวߪో㊀㊂ߩ㧟㧚㧥㧑ߢ޽ࠆ‫ޕ‬ᄢṚ᷷ᴰ᳓ߩ㨜㧴ߪ㧣㧚㧜㧢ߢ޽ߞߚ‫઀ޕ‬ㄟߺᓟߩ᷷ᐲ⚻ㆊߪ㧟㧜͠‫ޔ‬㧝ࡩ᦬᷷㉯ߩᓟ‫ޔ‬ಾࠅ㄰ߒࠍⴕ޿‫ޔ‬ቶ᷷㧔㧝㧜͠㨪㧞㧜͠㧕ߢߐࠄߦ㧠ࡩ᦬㑆ᾫᚑߒߚ‫ޕ‬ᾫᚑ⚳ੌᤨߦ৻⥸ᚑಽߣࡄࡀ࡞㧡ฬߦࠃࠆቭ⢻ᬌᩏࠍታᣉߒߚ‫ޕ‬‫⚿ޣ‬ᨐߣ⠨ኤ‫ޤ‬㧝㧚ᾫᚑ⚳ੌᤨߩ⦡߅ࠃ߮㨜* ⴫  ߦ␜ߒߚࠃ߁ߦᾫᚑ⚳ੌᤨߩ⦡ߩ඙ಽ㑆ߦᄢ߈ߥᏅ⇣ߪ⹺߼ࠄࠇߥ߆ߞߚ‫࠺ޕ‬࡯࠲࡯ߪ␜ߐߥ޿߇‫ޔ‬ᾫᚑਛߩ⌕⦡ߩㅴߺᣇߦ߽Ꮕߪ⹺߼ࠄࠇߥ߆ߞߚ‫ޕ‬ߎࠇࠄߩ⚿ᨐߪ᷷ᴰ᳓૶↪߇๧ཬᾫᚑߩㅴߺᣇߦᓇ㗀ࠍ෸߷ߒߡ޿ߥ޿ߎߣࠍ␜
ߒߡ޿ࠆ‫ޕ‬೨ㅀߒߚࠃ߁ߦ↪޿ߚᄢṚ᷷ᴰ᳓ߩ R* ߪ᳓㆏᳓㧔㨜*㧢㧚㧥㧥㧕ߣห᭽ߦਛᕈߢ޽ࠆߎߣ߆ࠄ઀ㄟߺ๧ཬߩ㨜* ߦ߶ߣࠎߤᏅ߇⊒↢ߒߥ߆ߞߚߎߣ߽‫ޔ‬ห᭽ߩ⌕⦡⚻ㆊࠍߣߞߚ৻࿃ߢ޽ࠆߣផ᷹ߐࠇࠆ‫ޕ‬ ⴫㧝 ᾫᚑ⚳ੌᤨߩ৻⥸ᚑಽ
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㪈㪈㪅㪉㪏㩷


㧞㧚㉂Უᢙ߅ࠃ߮ࠛ࠲ࡁ࡯࡞ߩᄌൻ㉂Უࠍᷝടߒߚ඙ಽ㧟㧔᷷ᴰ᳓ߥߒ㧕ߣ඙ಽ㧠㧔᷷ᴰ᳓޽ࠅ㧕ߩ㉂ᲣᢙߩᄌൻࠍᲧセߔࠆߣ‫߇ࠆ޽ߢ߆ߕࠊޔ‬඙ಽ㧟ߩ⩶ᢙ߇ᣧ߼ߦჇടߒ ⴫㧞 ‫↢ߩ࡞࡯ࡁ࠲ࠛߚ߹ޔ‬ᚑ߇ᄙ޿ߎߣ߇⹺߼ࠄࠇࠆ ⴫㧟 ‫⚿ߩߎޕ‬ᨐ߆ࠄ᷷ᴰ᳓ߩᷝട߇㉂Უߩ⊒㉂ࠍࠊߕ߆ߦ
ᛥ೙ߒߚน⢻ᕈ߇␜ໂߐࠇࠆ‫৻ߢߎߘޕ‬ㇱ᷷ᴰ᳓ࠍ฽߻㧔㧝㧜㧑‫ޔ‬㧞㧜㧑‫ޔ‬㧡㧜㧑㧕ᶧ૕ၭ࿾㧔㉂Უࠛࠠࠬ㧝㧑‫ࡦ࠻ࡊࡍ࡝ࡐޔ‬㧝㧑‫ࠬ࡯ࠦ࡞ࠣޔ‬㧡㧑‫ޔ‬㘩Ⴎ㧝㧜㧑㧕ࠍ↪
޿‫ޔ‬ᒰ࠮ࡦ࠲࡯଻ሽߩ⠴Ⴎᕈ㉂Უࠍ㧟㧜͠‫ޔ‬㧟ᣣ㑆‫ޔ‬ᶧ૕ၭ㙃ߒߚߣߎࠈ‫ޔ‬ኻᾖߩ⫳⇐᳓૶↪ၭ࿾ߩ↢⢒ߣỘᐲߦᏅߪߥߊ㧔࠺࡯࠲࡯⋭⇛㧕‫ޔ‬᷷ᴰ᳓߇⋥ធ⊛ߦ㉂Უߩ↢⢒ࠍᛥ೙ߐߖࠆߣߪ⹺߼ࠄࠇߥ߆ߞߚ‫ޕ‬᷷ᴰ᳓ߩ㉯ㅧᓸ↢‛ߦਈ߃ࠆᓇ㗀ߦߟ޿ߡߪ
੹ᓟߐࠄߦᬌ⸛߇ᔅⷐߣ⠨߃ࠄࠇࠆ‫ޕ‬− 36 −  ⴫㧞 ㉂Უᢙߩᄌൻ
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㩷㩷㪸㪾㫀㫅㪾㩷 㪻㪸㫐㫊㩷
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㪇㪅㪇㪊㩷 㩷㪇㪅㪇㪉㩷 㩷㪇㪅㪇㪉㩷 㩷㪇㪅㪇㪊㩷 㩷㪉㩷㪇㪅㪇㪉㩷 㩷㪇㪅㪇㪋㩷 㩷㪇㪅㪇㪋㩷 㩷㪇㪅㪇㪋㩷 㩷㪊㩷㪇㪅㪇㪎㩷 㩷㪈㪅㪋㪇㩷 㩷㪈㪅㪌㪍㩷 㩷㪈㪅㪌㪈㩷 㩷㪋㩷㪇㪅㪇㪍㩷 㩷㪇㪅㪌㪏㩷 㩷㪇㪅㪐㪇㩷 㩷㪈㪅㪈㪈㩷 㩷㧟㧚ࠕࡒࡁ㉄⚵ᚑ⴫㧠ߦᾫᚑ⚳ੌᤨߩ๧ཬߩࠕࡒࡁ㉄⚵ᚑࠍ␜ߒߚ‫ޕ‬㊂⊛ߦᄙ޿ࠕࡒࡁ㉄ߣߒߡ‫ࠣޔ‬࡞࠲ࡒࡦ㉄‫ࠕޔࡦࠫ࡝ޔ㉄ࡦࠡ࡜ࡄࠬࠕޔࡦ࠾࡜ࠕ࡞ࠚࡈޔࡦࠪࠗࡠޔࡦ࠾ࠡ࡞ࠕޔ‬࡜࠾ࡦ‫ࠍ㉄ࡁࡒࠕߩࠄࠇߎޔ߇ߚࠇࠄ߼⹺߇ࡦ࡝࠮ޔࡦࠪࠗࡠ࠰ࠗޔ‬฽߻ฦ⒳ࠕࡒࡁ
㉄ߦߟ޿ߡ߽඙ಽߦࠃࠆᏅߪ⹺߼ࠄࠇߥ߆ߞߚ‫ޕ‬㧠㧚ቭ⢻⹜㛎ߩ⚿ᨐ ⴫㧡ߦࡄࡀ࡞㧡ฬߦࠃࠆቭ⢻ᬌᩏߩᐔဋ⹏ὐࠍ␜ߒߚ‫ޕ‬᷷ᴰ᳓ࠍ↪޿ߚ๧ཬߦኻߔ
ࠆ⹏ଔߪࡄࡀ࡞ߦࠃߞߡ⇣ߥߞߡ޿ߚ߇‫ޔ‬ᐔဋ⊛ߦߪ᷷ᴰ᳓ߩ᦭ήߦࠃࠆᄢ߈ߥᏅߪ
⹺߼ࠄࠇߕ‫ޔ⦡ޔ‬๧‫ޔ‬㚅ࠅ‫⚵ޔ‬ᚑߩฦ㗄⋡ߦߟ޿ߡ᦭ᗧᏅ߽⹺߼ࠄࠇߥ߆ߞߚ‫✚ޕ‬ว⊛ߥ⹏ଔ߽᷷ᴰ᳓ࠍ↪޿ߚ๧ཬߪኻᾖߣ㆖⦡ߥ߆ߞߚ‫ ޕ‬੹࿁‫઀ޔ‬ㄟߺ᳓ߣߒߡᄢṚ᷷ᴰ᳓ࠍ↪޿ߚዊ઀ㄟߺ⹜㛎ߩ⚿ᨐ߆ࠄ‫⦟ޔ‬ᅢߥຠ⾰ߩ๧ཬ߇⵾ㅧߢ߈ࠆߎߣࠍ⏕⹺ߒߚ‫ޕ‬᷷ᴰ᳓߇ᾫᚑਛߦ㉂Უ╬ߩ㉯ㅧᓸ↢‛ߩ⊒㉂ߦਈ
߃ࠆᓇ㗀ߦߟ޿ߡߪ‫ⶄޔ‬ᢙ࿁ߩ઀ㄟߺ⹜㛎ࠍታᣉߔࠆߎߣߦࠃࠆ⹦⚦ߥᬌ⸛߇ᔅⷐߢ
޽ࠆߣ⠨ኤߐࠇࠆ‫੹߅ߥޕ‬࿁ߩ᷷ᴰ᳓૶↪ߪ⒳᳓ߩߺߢ޽ࠅ‫઀✚ޔ‬ㄟߺ㊀㊂ߩᢙߦߔ߉ߥ޿‫ޕ‬ේᢱಣℂⵝ⟎ߦ⽶ᜂߦߥࠆ߇‫ޔ‬ᄢ⼺ᶐẃᤨߦ߽૶↪ߔࠆߎߣߢߐࠄߦ᷷ᴰ
᳓ߩᓇ㗀߇‫ࠅࠃޔ‬᣿⏕ߦߥࠆߣ੍ᗐߐࠇࠆ‫੹ߚ߹ޕ‬࿁ߩ⹜㛎ߪ  ࠞᚲߩߺߩ᷷ᴰ᳓ߦ
ߟ޿ߡ⺞ߴߚ⚿ᨐߢ޽ࠅ‫੹ޔ‬ᓟ‫ߩઁޔ‬㘶↪น⢻ߥ᷷ᴰ᳓ߦߟ޿ߡߩᬌ⸛߽ᔅⷐߢ޽ࠆ‫ޕ‬ᣣᧄో࿖ߦߪᄙߊߩ᷷ᴰ߇ሽ࿷ߒߡ߅ࠅ‫ޔ‬㘶ᢱߣߒߡߩ᷷ᴰ᳓߇⃻࿷‫ޔ‬ᄙߊ⽼ᄁߐࠇߡ޿ࠆߎߣ߆ࠄ‫੹ޔ‬ᓟ‫ޔ‬๧ཬ╬ߩ⊒㉂㘩ຠߦ᷷ᴰ᳓߇೑↪ߐࠇ‫ޔ‬໡ຠߩᄙ᭽ൻߦߟߥ
߇ࠆน⢻ᕈ߇޽ࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬− 37 − ⴫㧠 ᾫᚑ⚳ੌᤨߩࠕࡒࡁ㉄⚵ᚑ OII 
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 ⴫㧡 ቭ⢻ᬌᩏߩ⚿ᨐ
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 㧖 ὐᴺ‫ޔ‬ᢙሼߩዊߐ޿ᣇ߇⦟ᅢ‫ޕ‬ ฦ㗄⋡ߣ߽᦭ᗧᏅߥߒ‫࡞ࡀࡄޕ‬㧡ฬ ‫⻢ޣ‬ㄉ‫ޤ‬
᷷ᴰ᳓ߥߤߩේ᧚ᢱࠍឭଏߒߡ޿ߚߛ޿ߚᄢ㙚ᏒᄢṚ᷷ᴰ࿾ၞᵴᕈൻද⼏ળ‫ޔ‬Ꮏ⮮ᷕ ᳁‫ޔࠬ࠙ࡂࡦ࠻࡝࠻ޔ‬㠽ẟ ഞ ᳁ߦᗵ⻢޿ߚߒ߹ߔ‫ޕ‬− 38 −

㧟㧚✚⺑Ԙ‫ޟ‬㘩ຠᚑಽߦࠃࠆࡔ࠲ࡏ࡝࠶ࠢࠪࡦ࠼ࡠ࡯ࡓ੍㒐࡮ᡷༀലᨐߦ㑐ߔࠆ⎇ⓥ‫ ̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ ޠ‬39
ᮘᷰ৻ਯ


樋渡一之

秋田県総合食品研究センター報告

Vol. 13, 39 − 53（2011）㘩ຠᚑಽ䈮䉋䉎䊜䉺䊗䊥䉾䉪䉲䊮䊄䊨䊷䊛੍㒐䊶ᡷༀലᨐ䈮㑐䈜䉎⎇ⓥᮘᷰ㩷 ৻ਯ
䋨⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䋩
Kazuyuki HIWATASHI䇼ⷐ⚂䇽㩷 ᧄ⎇ⓥ䈲䇮㘩ຠ䈮↱᧪䈜䉎䊜䉺䊗䊥䉾䉪䉲䊮䊄䊨䊷䊛੍㒐࿃ሶ䉕ត⚝䇮䈠䈱૞↪ᯏ᭴䉕᣿䉌䈎䈮䈜䉎䈖䈫䉕⋡⊛䈫䈚䈩ⴕ䉒䉏䈢䇯㩷 㜞⢽⢌㘩⽶⩄䊙䉡䉴䉕↪䈇䈩䊟䊙䊑䉲䉺䉬䈱⢽⾰ઍ⻢ᡷༀ૞↪䉕ᬌ⸛䈚䈢䈫䈖䉐䇮૕㊀Ⴧട䈱ᛥ೙䇮ⴊᷡਛᕈ⢽⢌Ớᐲ䈱ૐਅ䇮⢽⢌⢄ᡷༀ૞↪䈏⹺䉄䉌䉏䇮䈠䈱䊜䉦䊆䉵䊛䈱ዋ䈭䈒䈫䉅৻ㇱ䈲䊟䊙䊑䉲䉺䉬䈮฽䉁䉏䉎䊕䊦䉥䉨䉲䉹䊷䊛Ⴧᱺ೷ᔕ╵ᕈฃኈ૕㱍䋨PPAR䋩䉝䉯䊆䉴䊃䈏⢄⤳ਛ䈱⢽⾰ઍ⻢㑐ㅪㆮવሶ䈱⊒⃻䉕਄᣹䈘䈞䉎䈖䈫䈮䉋䉎䉅䈱䈪䈅䉎䈖䈫䈏␜ໂ䈘䉏䈢䇯䊟䊙䊑䉲䉺䉬䈱䊍䊃䈮䈍䈔䉎૞↪䉕ᬌ⸽䈚䈢䈫䈖䉐䇮䈠䈱ⴊਛਛᕈ⢽⢌ૐਅ૞↪
䈲᣿⏕䈮䈭䉌䈭䈎䈦䈢䇯㩷 䉻䉟䉵䈮฽䉁䉏䉎䊧䊆䊮㒖ኂ‛⾰䉕න㔌䈚᭴ㅧ䉕⸃ᨆ䈚䈢䈫䈖䉐䇮䉸䊟䉰䊘䊆䊮 I 䈫หቯ䈘䉏䈢䇯䉸䊟䉰䊘䊆䊮 I 䉕฽䉃䉻䉟䉵䉰䊘䊆䊮䈮䉋䉎ⴊ࿶਄᣹ᛥ೙૞↪䉕ᬌ⸛䈚䈢䈫䈖䉐䇮⚻ญᛩਈ䈮䉋䉍㜞ⴊ࿶⥄ὼ⊒∝䊤䉾䊃䈱ⴊ࿶਄᣹䉕ᛥ೙䈜䉎䈖䈫䈏␜䈘䉏䈢䈖䈫䈎䉌䇮䉻䉟䉵䉰䊘䊆䊮䈲 in vivo 䈮䈍䈇䈩䉅䊧䊆䊮ᵴᕈ䉕㒖ኂ䈜䉎䈖䈫䈏␜ໂ䈘䉏䈢䇯㩷 એ਄䈱⚿ᨐ䈮䉋䉍䇮ᧄ⺰ᢥ䈪䈲䊟䊙䊑䉲䉺䉬䈱⢽⾰ઍ⻢ᡷༀ૞↪䈫䇮䊧䊆䊮㒖ኂᚑಽ䈮䉋䉎ⴊ࿶਄᣹ᛥ೙૞↪䈱䊜䉦䊆䉵䊛䈱৻ㇱ䉕⸃᣿䈜䉎䈖䈫䈏䈪䈐䈢䇯䈖䉏䉌䈲᛫䊜䉺䊗䊥䉾䉪䉲䊮䊄䊨䊷䊛ᵴᕈ䉕ᜬ䈧㘩ຠᚑಽ䈪䈅䉍䇮䊜䉺䊗䊥䉾䉪䉲䊮䊄䊨䊷䊛䈱੍㒐䊶ᡷༀ䈮⾗䈜䉎น⢻ᕈ䈏䈅䉎䇯੹ᓟ䇮ᧄᚑಽ䉕↪䈇䈢ᣂ䈢䈭ᯏ⢻ᕈ㘩ຠ䈱㐿⊒䈏ᦼᓙ䈪䈐䉎䇯䇼✜⸒䇽㩷 䊜䉺䊗䊥䉾䉪䉲䊮䊄䊨䊷䊛䋨MS䋩䈫䈲䇮⢈ḩ䇮♧ዩ∛䇮䉟䊮䉴䊥䊮ᛶ᛫ᕈ䇮㜞ⴊ࿶䇮⢽⾰ઍ⻢⇣Ᏹ䉕ၮᧄ⊛䈭᭴ᚑⷐ⚛䈫䈚䇮䈖䉏䉌䈱∛ᘒ䈏ว૬䈜䉎䈖䈫䈮䉋䉍േ⣂⎬ൻ╬䈱᭽䇱䈭∛᳇䈱䊥䉴䉪䉕⋧ਸ਼⊛䈮㜞䉄䉎䈫䈇䈉∔ᖚ᭎ᔨ䈪䈅䉎䇯䈠䈱ቯ⟵䇮䈜䈭䉒䈤䈬䈱䉋䈉䈭ၮḰ䉕↪䈇䈩⸻ᢿ䈜䉎䈎䈮䈲࿖㓙⊛䈭㆑䈇䈏䈅䉎䇯ᣣᧄ䈮䈍䈇䈩䈲⣻ㇱ⢈ḩ䋨ౝ⤳⢽⢌㕙Ⓧ䈱ᜰᮡ䋩䈮ട䈋䈩䇮㜞ⴊ♧䇮㜞ⴊ࿶䇮⢽⾰ઍ⻢⇣Ᏹ䈱䈉䈤䋲䈧એ਄䉕ว૬䈚䈢⁁ᘒ䈫ቯ⟵䈘䉏䈩䈇䉎䇯ෘ↢ഭ௛⋭䈱ᐔᚑ 20 ᐕ࿖᳃ஜᐽ䊶ᩕ㙃⺞ᩏ䈮䉋䉎䈫䇮MS 䈱⹥ᒰ⠪䈫䈠䈱੍஻ァ䋨⣻ㇱ⢈ḩ䈮ട䈋䈩 1 㗄⋡એ਄⹥ᒰ䋩䈱ഀว䈲 40-74 ᱦ↵ᕈ䈪 51.5䋦䈮㆐䈚䈩䈍䉍䇮䉅䈲䉇␠ળ໧㗴ൻ䈚䈩䈇䉎䈫䈇䈦䈩䉋䈇䇯䈖䈱໧㗴䉕⸃᳿䈜䉎ᚻᲑ䈱 1 䈧䈫䈚䈩䇮ᣣᧄ䈪䈲 1990 ᐕઍ䈎䉌㘩ຠᚑಽ䈮䉋䈦䈩↢૕ᯏ⢻䈱⺞▵䉕ⴕ䈉ᯏ⢻ᕈ㘩ຠ䈱㐿⊒䈏ᵴ⊒ൻ䈚䈢䇯╩⠪䈱ൕോ䈜䉎⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䈪䉅䇮MS 䈱੍㒐䊶ᡷༀ䈮ነਈ䈜䉎㘩ຠ䈱㐿⊒䈮ข䉍⚵䉖− 39 −

䈪䈍䉍䇮䈖䉏䉁䈪䉺䊮䊘䊘ᩮ↱᧪‛⾰䊦䊕䉥䊷䊦 1-3)䉇䇸⑺↰⋵䈱⧎䇹䊐䉨䊉䊃䉡䈱⢽⾰ઍ⻢ᡷༀ૞↪ 4)䇮☨♰⊒㉂䉣䉨䉴㈩ว㘶ᢱ䈱ⴊ࿶਄᣹ᛥ೙૞↪ 5)䈭䈬䉕᣿䉌䈎䈮䈚䈩䈐䈢䇯MS
䈱੍㒐䉇ᡷༀ䉕⋡⊛䈫䈚䈢᛫ MS 㘩ຠ䈱⎇ⓥ㐿⊒䈲ᭂ䉄䈩㊀ⷐ䈎䈧␠ળ⊛ⷐ⺧䈱ᒝ䈇⺖㗴䈪䈅䉎䇯㩷 䈖䉏䉁䈪䇮ᄙ䈒䈱⎇ⓥᯏ㑐䈪᛫ MS ᵴᕈ䉕᦭䈜䉎㘩ຠᚑಽ䈱⎇ⓥ䈏ⴕ䉒䉏䈩䈐䈢䇯䈚䈎䈚䈭䈏䉌䇮䈖䉏䉁䈪䈮⺞ᩏ⎇ⓥ䈘䉏䈢㘩ຠᚑಽ䈲ήᢙ䈮䈅䉎㘩ຠ⾗Ḯ䈱৻ㇱ䈮ㆊ䈑䈝䇮੹ᓟ䉅⛮⛯䈚䈩⎇ⓥ䉕ᄙ᭽ൻ䈘䈞䈩䈇䈎䈭䈔䉏䈳䈭䉌䈭䈇䇯ᧄ⺰ᢥ䈪䈲 MS 䈱੍㒐䊶ᡷༀ䈮⾗䈜䉎䈖䈫䉕⋡ᜰ䈚䇮⑺↰⋵ౝ䈪↢↥䊶⽼ᄁ䈘䉏䉎㘩ຠ⾗Ḯ䈎䉌䉴䉪䊥䊷䊆䊮䉫䈘䉏䈢㘩↪ᜂሶ⩶䈱৻⒳䈪䈅䉎䊟䊙䊑䉲䉺䉬䋨Hericium erinaceum䇮HE䇮Fig. 1䋩䈱⢽⾰ઍ⻢ᡷༀ૞↪䈫䇮䉻䉟䉵䋨Glycine max䋩↱᧪ᚑಽ䈪䈅䉎䉻䉟䉵䉰䊘䊆䊮䈱ᣂ䈢䈭૞↪ᯏᐨ䈮䉋䉎ⴊ࿶਄᣹ᛥ೙
૞↪䈱⹦⚦䉕⸃᣿䈜䉎䈖䈫䉕⋡⊛䈫䈚䈩⎇ⓥ䉕ⴕ䈦䈢䇯䇼䊟䊙䊑䉲䉺䉬䈏ᜬ䈧⢽⾰ઍ⻢ᡷༀ૞↪䈱⊒⷗䈫䈠䈱૞↪ᯏᐨ䈱⸃᣿䇽6)䋱䋮㜞⢽⢌㘩⽶⩄䊙䉡䉴䈮䈍䈔䉎ᬌ⸛㩷 HE 䈲᧲䉝䉳䉝䈮䈍䈇䈩Ყセ⊛䊘䊏䊠䊤䊷䈭㘩↪䉨䊉䉮䈪䈅䉍䇮ၭ㙃⚦⢩䈮䈍䈔䉎␹⚻ᚑ㐳࿃ሶ䈱↢วᚑଦㅴ 7,8)䉇䇮ሶታ૕☳ᧃ䈱⚻ญᛩਈ䈮䉋䉎シᐲ⹺⍮∝ᖚ⠪䈱∝⁁ᡷༀ╬䈱↢ℂ૞↪ 9)䈏ႎ๔䈘䉏䈩䈇䉎䇯䈚䈎䈚䈭䈏䉌䇮䈖䉏䉁䈪䈱䈫䈖䉐⢽⾰ઍ⻢䈮㑐ㅪ䈜䉎૞↪
䈲⍮䉌䉏䈩䈇䈭䈇䇯䈠䈖䈪 HE 䈱᛽಴‛䉕⺞⵾䈚䇮㜞⢽⢌㘩⽶⩄䊙䉡䉴䈮ᷙ㙄ᛩਈ䈚䈩 HE䈱⢽⾰ઍ⻢ᡷༀ૞↪䈮䈧䈇䈩ᬌ⸛䈚䈢䇯㩷 HE 䈱ᾲ㘑ੇ῎☳ᧃ䈱ᾲ᳓᛽಴ᶧ䉕ๆᒁỹㆊᓟ䇮⌀ⓨಓ⚿ੇ῎䈚䈩ᾲ᳓᛽಴‛䋨HW-E䋩
䈫䈚䈢䇯ห᭽䈮䉣䉺䊉䊷䊦᛽಴ᶧ䉕ๆᒁỹㆊᓟ䇮䊨䊷䉺䊥䊷䉣䊋䊘䊧䊷䉺䊷䈪䉣䉺䊉䊷䊦䉕⇐෰䈚䈢䉅䈱䉕䉣䉺䊉䊷䊦᛽಴‛䋨EtOH-E䋩䈫䈚䈢䇯8 ㅳ㦂䈱 C57BL/6J 䊙䉡䉴䈮䇮㜞⢽⢌㘺ᢱ䋨C ⟲䋩䇮HW-E 䉕 2%฽䉃㜞⢽⢌㘺ᢱ䋨HW ⟲䋩䇮EtOH-E 䉕 2%฽䉃㜞⢽⢌㘺ᢱ䋨EtOH ⟲䋩䉕⥄↱៨㘩䈘䈞䈢䋨Table 1䋩䇯4 ㅳ㑆䈱㘺⢒ᓟ䇮16 ᤨ㑆⛘㘩䈘䈞䈩⸃೬䊶ណⴊ䈮ଏ䈚䇮⚵❱㊀㊂䇮ฦ⒳ⴊᶧ↢ൻቇ୯䉕᷹ቯ䈚䈢䇯㩷 3 ⟲㑆䈱䊙䉡䉴䈱៨㘩㊂䈮䈲䈾䈫䉖䈬Ꮕ䈏䈭䈎䈦䈢䈮䉅䈎䈎䉒䉌䈝䋨Table 1䋩䇮C ⟲䈮Ყ䈼
䈩 HW ⟲䇮EtOH ⟲䈪᦭ᗧ䈮૕㊀Ⴧട䈏ᛥ೙䈘䉏䈢䋨Fig. 2䋩䇯HW ⟲䇮EtOH ⟲䈪䈲䇮⢄⤳䈫⣺㑆⤑⢽⢌⚵❱䈱㊀㊂䈏᦭ᗧ䈮ᷫዋ䈚䇮ⴊᷡਛᕈ⢽⢌Ớᐲ䉅᦭ᗧ䈮ૐਅ䈚䈢䇯䈘䉌䈮 HW⟲䇮EtOH ⟲䈪⢄⤳ਛ䈱ਛᕈ⢽⢌฽㊂䈏ᷫዋ䈚䈩䈍䉍䇮HE ᛽಴‛䈱⢽⢌⢄ᡷༀ૞↪䈏⹺䉄䉌䉏䈢䋨Table 2䋩䇯એ਄䈱⚿ᨐ䉋䉍䇮HE 䈲㜞⢽⢌㘩⽶⩄䊙䉡䉴䈱⢽⾰ઍ⻢䉕ᡷༀ䈜䉎䈖䈫䈏␜䈘䉏䈢䇯
䋲䋮⢽⾰ઍ⻢ᡷༀ䊜䉦䊆䉵䊛䈱⸃ᨆ㩷 ೨ㅀ䈱ㅢ䉍␜䈘䉏䈢⢽⾰ઍ⻢ᡷༀ૞↪䈮䈧䈇䈩䇮䈠䈱૞↪ᯏᐨ䉕ᬌ⸛䈚䈢䇯ⴊਛ䉇⢄⤳ਛ䈱ਛᕈ⢽⢌Ớᐲ䈏ૐਅ䈜䉎䈫䈇䈉⃻⽎䈲䇮⢄⤳䈪㱎㉄ൻ♽䉕ม䉎䊥䉧䊮䊄⚿วဳォ౮࿃ሶ䈪䈅䉎䊕䊦䉥䉨䉲䉹䊷䊛Ⴧᱺ೷ᔕ╵ᕈฃኈ૕㱍䋨PPAR䇮Fig. 3䋩10)䈱䉝䉯䊆䉴䊃䈪䈅䉎䊐䉞䊑䊤䊷䊃♽⮎೷䈱૞↪䈫㘃ૃ䈚䈩䈇䉎䇯䈠䈖䈪䊧䉶䊒䉺䊷䊶䉮䊐䉜䉪䉺䊷䉝䉾䉶䉟䉲䉴䊁䊛䉕↪䈇䈩䇮HE ᛽಴‛䈱 PPAR 䉝䉯䊆䉴䊃ᵴᕈ䉕᷹ቯ䈚䈢䈫䈖䉐䇮EtOH-E 䈮↪㊂ଐሽ⊛䈭䉝䉯− 40 −

䊆䉴䊃ᵴᕈ䈏⹺䉄䉌䉏䈢䋨Fig. 4䋩䇯
㩷 䈘䉌䈮㜞⢽⢌㘩⽶⩄䊙䉡䉴䈱⢄⤳䈮䈍䈔䉎䇮C ⟲䈫 EtOH ⟲䈱 PPAR ㆮવሶ䈍䉋䈶
PPAR 䈮䉋䈦䈩೙ᓮ䈘䉏䉎⢽⾰ઍ⻢㑐ㅪㆮવሶ䈱⊒⃻㊂䈱ᄌൻ䉕ቯ㊂ RT-PCR ᴺ䈪⸃ᨆ䈚䈢䇯䈠䈱⚿ᨐ䇮PPAR ⥄り䈱 mRNA ㊂䈮䈲ᄌൻ䈏⹺䉄䉌䉏䈭䈎䈦䈢䈏䇮PPAR 䈮䉋䈦䈩೙ᓮ䈘䉏䉎䈇䈒䈧䈎䈱ㆮવሶ䈱 mRNA ⊒⃻㊂䈏᦭ᗧ䈮Ⴧട䈚䈩䈇䈢䋨Fig. 5䋩䇯એ਄䈱⚿ᨐ䈮䉋䉍䇮EtOH-E 䈮䉋䉎૞↪䈱ዋ䈭䈒䈫䉅৻ㇱ䈲 HE 䈮฽䉁䉏䉎 PPAR 䉝䉯䊆䉴䊃䈏⢄⤳ਛ䈱⢽⾰ઍ⻢㑐ㅪㆮવሶ䈱⊒⃻䉕਄᣹䈘䈞䉎䈖䈫䈮䉋䉎䉅䈱䈪䈅䉎䈖䈫䈏␜ໂ䈘䉏䈢䇯䇼䊟䊙䊑䉲䉺䉬䈏ᜬ䈧ⴊਛਛᕈ⢽⢌ૐਅ૞↪䈱䊍䊃䈮䈍䈔䉎ᬌ⸽䇽㩷 HE 䈱␜䈜⢽⾰ઍ⻢ᡷༀ૞↪䈏䊍䊃䈮䈍䈇䈩䉅᦭ല䈎ุ䈎䉕ᬌ⸽䈜䉎䈢䉄䇮ⴊਛਛᕈ⢽⢌୯䉕ᜰᮡ䈫䈚䈢ዊⷙᮨ䈭䊒䊤䉶䊗ኻᾖੑ㊀⋤ᬌ䊤䊮䉻䊛ൻᲧセ⹜㛎䉕ⴕ䈦䈢䇯1 ㍤ 300
mg 䈮 HE ੇ῎☳ᧃ 100 mg 䉕฽䉃䉰䊒䊥䊜䊮䊃䉕⺞⵾䈚䇮HE ੇ῎☳ᧃ䉕䉳䊞䉧䉟䊝䊂䊮䊒䊮䈮⟎䈐឵䈋䈢䉅䈱䉕䊒䊤䉶䊗䈫䈚䈢䇯30䌾65 ᱦ䈪ⴊਛਛᕈ⢽⢌୯䈏 150䌾400 mg/dL 䈱ᣣᧄੱ↵ᕈ 21 ฬ䉕ⵍ㛎⠪䈫䈚䇮䊒䊤䉶䊗⟲ 11 ฬ䇮HE ⟲ 10 ฬ䈫䈭䉎䉋䈉ή૞ὑ䈮ഀ䉍ઃ䈔䈢䇯ⵍ㛎⠪䈮䈲 8 ㅳ㑆䇮Ფ㘩೨䈮 3 ㍤䈝䈧䉰䊒䊥䊜䊮䊃䉕៨ข䋨9 tablets/day䋩䈘䈞䇮⹜㛎㐿ᆎ೨䇮
4䇮8 ㅳ㑆⚻ㆊᤨὐ䈮䈍䈇䈩り㐳䊶૕㊀╬䈱૕᭴ㅧ᷹ቯ䇮ⴊ࿶䊶⣂ᜉ᷹ቯ䇮ᩕ㙃៨ข㊂ផ
ቯ䇮ⴊᶧᬌᩏ䉕ⴕ䈦䈢䇯㩷 ૕᭴ㅧ䇮ⴊ࿶䊶⣂ᜉ䇮ᩕ㙃៨ข㊂䈲⟲㑆䈍䉋䈶⹜㛎㐿ᆎ೨ᓟ䈱Ყセ䈪᦭ᗧ䈭Ꮕ䈏⹺䉄䉌䉏䈭䈎䈦䈢䇯ⴊᶧᬌᩏ୯䈪䈲૗ὐ䈎䈱᦭ᗧ䈭Ꮕ䈏⹺䉄䉌䉏䈢䈏䇮ਛᕈ⢽⢌䉕㒰䈇䈩ో䈩ၮḰ୯ౝ䈪䈱ᄌേ䈪䈅䉍⥃ᐥ⊛䈮ᗧ๧䈱䈅䉎Ꮕ䈲⹺䉄䉌䉏䈭䈎䈦䈢䋨Table 3䋩䇯䉰䊒䊥䊜䊮䊃៨ข䈮䉋䉎᦭ኂ੐⽎䈲⹺䉄䉌䉏䈝䇮੹࿁䈱ᛩਈ㊂䈮䈍䈇䈩䈲 HE ☳ᧃ䈱⚻ญᛩਈ䈲቟ోᕈ䈏㜞䈇䈖䈫䈏␜䈘䉏䈢䇯৻ᣇ䇮HE ⟲䈮䈍䈔䉎ⴊਛਛᕈ⢽⢌䈲䇮៨ข೨䈫Ყセ䈚䈩 4 ㅳᓟ᦭ᗧ䈮ૐਅ䈚䈩䈇䈢䈏䋨P = 0.013䋩䇮䊒䊤䉶䊗⟲䈫䈱Ყセ䈪䈲᦭ᗧᏅ䈲⹺䉄䉌䉏䈭䈎䈦䈢䋨P =
0.127䋩䋨Fig. 6䋩䇯એ਄䈱⚿ᨐ䈎䉌䇮HE 䈱䊍䊃䈮䈍䈔䉎ⴊਛਛᕈ⢽⢌ૐᷫ૞↪䈲᣿⏕䈮䈭䉌䈭䈎䈦䈢䈏䇮᦭ല䈪䈅䉎น⢻ᕈ䉅৻ㇱ␜ໂ䈘䉏䈢䇯䇼㘩ຠ↱᧪䊧䊆䊮㒖ኂ‛⾰䈮䉋䉎ⴊ࿶਄᣹ᛥ೙૞↪䇽䋱䋮䊧䊆䊮㒖ኂ‛⾰䈱หቯ䈫 in vitro 䈮䈍䈔䉎ᵴᕈ㒖ኂ⹏ଔ 11)㩷 䊧䊆䊮䋨EC 3.4.23.15䋩䈲䇮䉝䊮䉳䉥䊁䊮䉲䊉䊷䉭䊮䈎䉌䉝䊮䉳䉥䊁䊮䉲䊮 I 䉕ಾᢿ䈜䉎䉝䉴䊌䊦䊁䉞䉾䉪䊒䊨䊁䉝䊷䉷䈪䈅䉎䇯䈖䈱෻ᔕ䈏㊀ⷐ䈭ⴊ࿶⺞▵ᯏ᭴䈱৻䈧䈪䈅䉎䊧䊆䊮䋭䉝䊮䉳䉥䊁䊮䉲䊮♽䈱ᓞㅦᲑ㓏䈫䈭䈦䈩䈇䉎䋨Fig. 7䋩12)䇯ㄭᐕ䇮⚻ญᛩਈ䈪᦭ല䈭䊧䊆䊮㒖ኂ೷䈫䈚䈩㐿⊒䈘䉏䈢䉝䊥䉴䉨䊧䊮䈏ᣣ☨᰷䈪㜞ⴊ࿶ᴦ≮೷䈫䈚䈩䈱⹺น䉕ฃ䈔䈩䈇䉎 13)䇯䈠䈖
䈪䊋䉨䊠䊨䉡䉞䊦䉴䋭᣸⯻⚦⢩♽䈪ᓧ䈢䊍䊃ဳ⚵឵䈋䊧䊆䊮䈫ᶖశᕈⰯశၮ⾰䉕⚵䉂ว䉒䈞䈢䊧䊆䊮㒖ኂᵴᕈ䈱᷹ቯᴺ䋨Fig. 8䋩14,15)䉕↪䈇䇮㘩ຠ⚛᧚䈎䉌䊧䊆䊮㒖ኂᚑಽ䉕䉴䉪䊥䊷䊆䊮䉫䈚䈢䈫䈖䉐䇮䉻䉟䉵䈮䊧䊆䊮㒖ኂᵴᕈ䉕⹺䉄䈢䇯㩷 䊧䊆䊮㒖ኂ‛⾰䉕หቯ䈜䉎䈢䉄䇮䉻䉟䉵䈱ᾲ᳓᛽಴‛䉕⺞⵾䈚䈩ฦ⒳䉪䊨䊙䊃䉫䊤䊐䉞䊷䈮䉋䉍㒖ኂ‛⾰䉕න㔌䇮᭴ㅧ⸃ᨆ䈚䈢䈫䈖䉐䇮䉻䉟䉵䈮Ყセ⊛ᄙ䈒฽䉁䉏䉎䉰䊘䊆䊮䈪䈅䉎䉸䊟䉰䊘䊆䊮 I 䈪䈅䉎䈖䈫䈏᣿䉌䈎䈫䈭䈦䈢䋨Fig. 9䋩䇯↪㊂ଐሽᕈ䈮䈧䈇䈩ᬌ⸛䈚䈢䈫䈖䉐 50%㒖− 41 −

ኂỚᐲ䈲 30 μg/mL 䈪䈅䈦䈢䇯䋲䋮㜞ⴊ࿶⥄ὼ⊒∝䊤䉾䊃䈮䈍䈔䉎ᬌ⸛ 16)㩷 䉻䉟䉵ਛ䈱䊧䊆䊮㒖ኂ‛⾰䈏䉸䊟䉰䊘䊆䊮 I 䈫หቯ䈘䉏䈢䈖䈫䈎䉌䇮䉸䊟䉰䊘䊆䊮 I 䉕฽䉃䈫⠨䈋䉌䉏䉎Ꮢ⽼✚䉻䉟䉵䉰䊘䊆䊮䋨TSS䇮๺శ⚐⮎Ꮏᬺ␠⵾䉻䉟䉵↱᧪䉰䊘䊆䊮䋩䈱 in vitro䈪䈱䊧䊆䊮㒖ኂᵴᕈ䉕⹏ଔ䈚䈢䇯䈠䈱⚿ᨐ䇮TSS 䈲↪㊂ଐሽ⊛䈮䊧䊆䊮䉕㒖ኂ䈜䉎䈖䈫䈏␜
䈘䉏䋨Fig. 10䋩䇮䈠䈱 50%㒖ኂỚᐲ䈲 59.9 μg/mL 䈪䈅䈦䈢䇯㩷 䈠䈖䈪 TSS 䉕㜞ⴊ࿶⥄ὼ⊒∝䊤䉾䊃䋨SHR䋩䈮⚻ญᛩਈ䈚䇮䈠䈱 in vivo 䈮䈍䈔䉎૞↪䉕ᬌ⸽䈚䈢䇯TSS 䉕 40 mg/mL 䈫䈭䉎䉋䈉䈮⫳⇐᳓䈪ṁ⸃䈚䇮䈖䉏䉕 TSS ṁᶧ䈫䈚䈢䇯10 ㅳ㦂䈱㓶
ᕈ SHR/Izm 䈮Ꮢ⽼࿕ᒻ㘺ᢱ䉕⥄↱៨㘩䈘䈞䇮8 ㅳ㑆㘺⢒䈚䈢䇯㘺⢒ᦼ㑆ਛᲤᣣ䇮2 mL/kg
BW 䈱 TSS ṁᶧ䋨TSS ⟲䋩䉁䈢䈲⫳⇐᳓䋨C ⟲䋩䉕ᒝ೙⚻ญᛩਈ䈚䇮1 ㅳ㑆䈮 1 ࿁૕㊀䇮៨㘩㊂䈫ⴊ࿶䉕᷹ቯ䈚䈢䇯㘺⢒⚳ੌᓟ䇮16 ᤨ㑆⛘㘩䈘䈞䈩⸃೬䊶ណⴊ䈮ଏ䈚䇮⚵❱㊀㊂䇮ฦ⒳ⴊᶧ↢ൻቇ୯䉕᷹ቯ䈚䈢䇯⹜㛎ᦼ㑆䉕ㅢ䈚䈩䇮૕㊀䈍䉋䈶៨㘩㊂䈮Ꮕ䈲⹺䉄䉌䉏䈭䈎䈦䈢䇯䉁䈢㘺⢒⚳ੌᤨ䈱⚵❱㊀㊂䉅ో䈩䈱⚵❱䈪Ꮕ䈲⹺䉄䉌䉏䈭䈎䈦䈢䇯෼❗ᦼⴊ࿶䈲䇮C ⟲䈮ኻ䈚䈩 TSS ⟲䈪⹜㛎㐿ᆎ 1 ㅳ⋡䈎䉌ૐ୯䉕␜䈚䇮7䇮8 ㅳ⋡䈪᦭ᗧ䈭਄᣹ᛥ೙䈏⹺䉄䉌䉏䈢
䋨Fig. 11䋩䇯ⴊᶧ↢ൻቇ୯䈪䈲䇮C ⟲䈫Ყセ䈚䈩 LDL 䉮䊧䉴䊁䊨䊷䊦䇮AST䇮ALT 䈪 TSS ⟲䈏᦭ᗧ䈭ૐ୯䉕␜䈚䈢䈱䈮ട䈋䇮⣢ᯏ⢻䊙䊷䉦䊷䈪䈅䉎ⴊᶧዩ⚛⓸⚛䈪䉅᦭ᗧ䈭ૐਅ䈏⹺䉄䉌䉏䈢䋨Table 4䋩䇯એ਄䈱⚿ᨐ䈮䉋䉍䇮TSS 䈱⚻ญᛩਈ䈲 SHR 䈱ⴊ࿶਄᣹䉕ᛥ೙䈜䉎䈖䈫䈏␜䈘䉏䇮TSS 䈲 in vivo 䈮䈍䈇䈩䉅䊧䊆䊮ᵴᕈ䉕㒖ኂ䈜䉎䈖䈫䈏␜ໂ䈘䉏䈢䇯䇼⻢ㄉ䇽㩷 ᧄ✚⺑䈲䇮╩⠪䈱᧲ർᄢቇᄢቇ㒮ㄘቇ⎇ⓥ⑼↢‛↥ᬺഃᚑ⑼ቇኾ᡹䈮䈍䈔䉎ඳ჻⺰ᢥ䈱ౝኈ䉕䉁䈫䉄䈢䉅䈱䈪䈜䇯ඳ჻⺖⒟䈻䈱࿷☋䈮䈅䈢䉍䇮⑺↰⋵⎇ⓥ⡯ຬᄢቇ㒮ඳ჻ᓟᦼ⺖⒟⎇ୃᡰេ੐ᬺ⵬ഥ㊄䈱ᡰេ䉕ฃ䈔䉁䈚䈢䇯⑺↰⋵䈍䉋䈶㑐ଥฦ૏䈮ᷓ䈒ᗵ⻢䈇䈢䈚䉁䈜䇯㩷 ⎇ⓥ䈱ㆀⴕ䈫⺰ᢥ䈱ၫ╩䈮䈅䈢䉍䇮ਥᩏ䉕䈍㗿䈇䈇䈢䈚䉁䈚䈢᧲ർᄢቇᄢቇ㒮ㄘቇ⎇ⓥ⑼ᢎ᝼㩷 㚤੗ਃජᄦඳ჻䇮೽ᩏ䉕䈗ᜂᒰ䈇䈢䈣䈇䈢หᄢቇ㒮↢૕ಽሶᯏ⢻ቇಽ㊁ᢎ᝼㩷ᳰ↰㇚↵ඳ჻䇮േ‛⾗Ḯൻቇಽ㊁ᢎ᝼㩷 㥲⮮ᔘᄦඳ჻䇮ᩕ㙃ቇಽ㊁ಎᢎ᝼㩷 ⊕Ꮉੳඳ჻䈮䈲ᄙ䈒䈱᦭⋉䈭ഥ⸒䈫䈗ᜰዉ䉕㗂䈐䉁䈚䈢䇯䈖䈖䈮ෘ䈒ᓮ␞↳䈚਄䈕䉁䈜䇯㩷 ᧄ⎇ⓥ䈱ᄢㇱಽ䈲⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䈪ⴕ䉒䉏䉁䈚䈢䇯㘩ຠടᎿ⎇ⓥᚲ㐳㩷㜞ᯅ⍾❱ඳ჻䇮਄Ꮸ⎇ⓥຬ㩷 ၳ৻ਯඳ჻䇮ਥછ⎇ⓥຬ㩷 ᾢ⼱᣽ೣඳ჻䇮ਥછ⎇ⓥຬ㩷 ⇗ᕺมඳ჻䉕䈲䈛䉄䈫䈜䉎⡯ຬ䈱⊝᭽䈮ᓮᜰዉ䇮ᓮදജ䇮ỗബ䉕⾦䉍䉁䈚䈢䇯ෘ䈒ᓮ␞↳䈚਄䈕䉁䈜䇯㩷 ᧄ⎇ⓥ䉕ⴕ䈉䈮䈅䈢䉍䇮ᩣᑼળ␠ဈᧄ䊋䉟䉥ઍ⴫ข✦ᓎ㩷 ဈᧄ⾫ੑඳ჻䇮⑺↰⋵┙ᄢቇᢎ᝼㩷 ⑺ጊ⟤ዷඳ჻䇮⍹Ꮉ⋵┙ᄢቇಎᢎ᝼㩷 ศၔ↱⟤ሶඳ჻䈾䈎䇮ᄙ䈒䈱ᣇ䇱䈱䈗ᜰዉ䇮䈗ഥ⸒䉕䈇䈢䈣䈐䉁䈚䈢䇯ో䈩䈱ᣇ䈱䈍ฬ೨䉕⸥䈜䈖䈫䈲䈪䈐䉁䈞䉖䈏䇮䈗දജ䈇䈢䈣䈇䈢ో䈩䈱ᣇ䈮ᷓ䈒ᗵ⻢䈇䈢䈚䉁䈜䇯㩷 ᧄ⎇ⓥ䈱⎇ⓥ⾗㊄䈱৻ㇱ䈲䇮ᢥㇱ⑼ቇ⋭ㇺᏒ䉣䊥䉝↥ቇቭㅪ៤ଦㅴ੐ᬺ䋨⑺↰⋵ᄩ䉣䊥− 42 −

䉝䋩䈱ഥᚑ䈮䉋䉎䉅䈱䈪䈜䇯ᗵ⻢䈇䈢䈚䉁䈜䇯㩷 ᦨᓟ䈮䇮᡿દ⮮⟵ᢥඳ჻䈮ᧄ⺰ᢥ䉕ᝤ䈕䉁䈜䇯

Table 1 Compositions and food intake of experimental
diets based on the high-fat diet.
Ingredients (g/ 100g)
C
Casein24.50
Egg white
5.00
L-Cystine
0.43
Beef tallow
15.88
Safflower oil
20.00
Cellulose
5.50
Maltodextrin
8.25
Lactose
6.93
Sucrose6.75
Vitamin mix (AIN-93)
1.40
Mineral mix (AIN-93G)
5.00
Choline bitartrate
0.36
0.002
tert-Butylhydoroquinone
Hot water extract of Yamabushitake
EtOH extract of Yamabushitake
Actual compositions (g/100g)
Moisture
Protein
Lipid
Ash
Carbohydrate
Dietary fiber
Food intake (g/d)

6.1
25.4
32.7
3.6
32.2
5.5
2.56

HW
24.01
4.90
0.42
15.56
19.60
5.39
8.09
6.79
6.62
1.37
4.90
0.35
0.002
2.00

EtOH
24.01
4.90
0.42
15.56
19.60
5.39
8.09
6.79
6.62
1.37
4.90
0.35
0.002
2.00

5.0
26.3
31.2
3.9
33.6
5.8
2.37

7.6
26.6
29.3
3.7
32.8
5.5
2.52

C, control diet; HW, hot water extract diet; EtOH, ethanol extract diet− 43 −

Body weight (g)

40

C
HW
EtOH

35

*

**

30

**

25
0

10
20
Experimental period (day)30

Fig. 2 Effect of experimental diets on body weightgain in mice fed a high-fat diet. Symbols indicate
control diet group (C, open circle), hot water extract
diet group (HW, closed square), and ethanol extract
diet group (EtOH, closed triangle). Values aremeans with standard errors, depicted by vertical bars
(six mice per group). Mean values weresignificantly different from those of the control
group: *P < 0.05; **P < 0.01.

Fig. 3 Metabolic integration by PPARs. The three
PPAR isoforms regulate lipid and glucose
homeostasis through their coordinated activities inliver, muscle and adipose tissue.

− 44 −

Table 2 Effect of experimental diets on the tissue weight and the biochemical parameters in mice fed ahigh-fat diet.
C
Mean
Tissue weight
Liver

(mg)
(mg/ 100g BW)
Mesenteric adipose tissue (mg)(mg/ 100g BW)
Epididymal adipose tissue (mg)(mg/ 100g BW)
Serum
Triglyceride(mg/ dL)
Total cholesterol(mg/ dL)HDL cholesterol(mg/ dL)LDL cholesterol(mg/ dL)
NEFA(mEq/ L)
Glucose(mg/ dL)
AST(U/ L)ALT(U/ L)
Adiponectin(μg/ ml)
Leptin(ng/ ml)
Liver
Triglyceride(mg/ g liver)
Total cholesterol(mg/ g liver)Phospholipid(mg/ g liver)

HW
SEM

Mean

EtOH
SEM

Mean

SEM

1485
4180
1056
2968
846
2387

87
182
76
165
39
89

1170*
3582
714*
2173*
721
2215

62
109
73
158
33
83

1183*
3793
649*
2067*
698
2243

89
189
79
202
37
72

122.5
176.2
107.0
44.7
1.4
269.5
141.8
50.2
20.9
74.8

7.7
5.5
1.6
4.0
0.1
8.1
10.5
5.2
1.0
4.4

112.7*
156.3
104.8
29.2
1.5
210.7*
129.7
36.8
22.9
60.7

3.2
6.6
2.0
4.5
0.1
8.1
12.2
1.9
1.8
13.0

89.3**
162.2
107.7
36.8
1.2
225.2
167.0
36.4
23.5
43.0

7.6
8.3
1.6
6.2
0.1
14.8
22.4
5.1
2.7
10.0

160.2
3.6
14.3

12.2
0.2
0.4

112.5*
3.9
15.3

11.8
0.3
0.3

98.1**
3.4
16.3**

15.4
0.2
0.2

Relative activity (%)

C, control diet; HW, hot water extract diet; EtOH, ethanol extract diet; NEFA, nonesterified fatty acid; AST, aspartate aminotransferase;
ALT, alanine aminotransferase. Mean values with standard errors for six mice per group. Mean values were significantly different fromthose of C: *P < 0.05, **P < 0.01

10080

Wy14643
EtOH
HW

60
40
20
0
10-9 10-8 10-7 10-6 10-5 10-4 10-3
Wy14643 concentration (M)Extract concentration (g/mL)

Fig. 4 Dose-response curve of Yamabushitake
extracts for PPAR㱍 agonist. PPAR㱍 agonist
activity was measured by the PPAR㱍/Coactivator
Ligand Assay System. Wy14643, a selective
PPAR㱍 agonist, was used as positive control.
Relative activity is shown, with the maximum
activity of Wy14643 as 100%. Symbols indicate
Wy14643 (open circle), EtOH extract (EtOH, closed
triangle), and hot water extract (HW, closed square).
Values are the means of duplicate analysis.

− 45 −

A

C

1.5

1.5

*

Relative expression

**
C

1.0

1.0

0.5

0.5

0

0

EtOH

Srebf1

Ppara

Fasn

Acaca

B
*

1.5
Relative expression

*

*

*

Acox1 Acadm

Acadl

1.0
**

0.5

0
Apoa1

Lpl Slc27a1 Slc27a4 Acsl1

Fig. 5 Effects of experimental diets on mRNA
expression of PPAR㱍 (A), its regulating genes (B),and other lipid metabolic genes (C) in the liver.
mRNA levels were analyzed by quantitative RTPCR, the amount of mRNA normalized with 㱎actin mRNA as endogenous control. Columns
indicate the control diet group (C, open column),
and the ethanol extract diet group (EtOH, closed
column). Values are means, and standard errors are
depicted by vertical bars (six mice per group). Meanvalues were significantly different from those of thecontrol group: *P < 0.05; **P < 0.01. Ppara,
peroxisome proliferator-activated receptor 㱍(PPAR㱍); Apoa1, apo A-I; Lpl, lipoprotein lipase;Slc27a1, fatty acid transport protein 1; Slc27a4, fatty
acid transport protein 4; Acsl1, acyl-CoA synthetaselong-chain family member 1; Acox1, palmitoyl acylCoA oxidase 1; Acadm, acyl-CoA dehydrogenase,medium chain; Acadl, acyl-CoA dehydrogenase,long-chain; Srebf1, sterol regulatory elementbinding factor 1; Fasn, fatty acid synthase; Acaca,
acetyl-CoA carboxylase 㱍.

− 46 −

Plasma triglyceride (mg/ dL)placebo group
Yamabushitake group

300

200

*

100

0
0

4
Experimental period (weeks)

8Fig. 6 Effect of oral Yamabushitake administration
on plasma triglyceride in mild hypertriglyceridemic
men. Symbols indicate placebo group (open circle)
and Yamabushitake group (closed square). Values
are means, and standard errors are depicted byvertical bars (10 or 11 men per group). Mean values
were significantly different from those of the zero
week: *P < 0.05.

− 47 −

Table 3 Effect of oral Yamabushitake
administration on physical parameters, bloodpressure, and blood parameters in mild
hypertriglyceridemic men.
Week 0
Mean
SEM
P
55.1
2.2
Muscle masskg
HE 52.8
2.2
P
21.2
2.4
Fat masskg
HE 15.9
2.6
P
79.5
4.0
Body weightkg
HE 71.6
4.3
P
26.2
1.8
Percentage of body fat%
HE 21.1
2.3
P
27.2
1.2
Body mass index
㧙
HE 24.0
1.3
P
114
4.2
SBP
mmHg
HE
117
5.1
P
69
2.4
DBP
mmHg
HE
73
4.7
P
65.7
1.9
pulse
/min
HE 70.5
1.8
P 6766
517
White blood cell/L
HE 6907
384
P
520
9.3
4
Red blood cell×10 /L
HE
505
5.1
P
15.7
0.2
Hemoglobin
g/dL
HE 15.2
0.3
P
46.7
0.7
Hematocrit%
HE 45.6
0.6
P
89.8
1.1
MCV
fL
HE 90.3
0.9
P
30.3
0.4
MCH
pg
HE 30.1
0.3
P
33.7
0.3
MCHC
%
HE 33.4
0.2
P
23.3
1.4
4
Platelet
×10 /L
HE 26.3
1.3
P
222
8
Total cholesterol
mg/dL
HE
218
7
P
198
17
Triglyceride
mg/dL
HE
193
11
P
139
11
LDL cholesterol
mg/dL
HE
143
8
P
46.7
2.6
HDL cholesterol
mg/dL
HE 49.6
2.0
P
12.4
0.8
Blood urea nitrogen mg/dL
HE 14.1
1.2
P
0.81
0.02
Creatinine
mg/dL
HE 0.75
0.04
P
7.05
0.31
Uric acid
mg/dL
HE 6.17
0.52
P
25.5
2.5
AST
U/L
HE 24.4
2.9
P
36.2
4.5
ALT
U/L
HE 31.3
6.5
P
53.8
6.4
-GT
U/L
HE 39.7
7.4
P
96.5
2.7
Glucose
mg/dL
HE
101
2
P
0.60
0.07
NEFA
mEq/L
HE 0.43
0.05− 48 −

Week 4
Mean
SEM
55.6
2.1
53.1
2.1
20.8
2.5
15.5
2.6
79.5
3.9
71.6
4.3
25.6
1.8
20.5
2.4
27.3
1.2
24.1
1.3

6685
7429
517
503
15.7
15.1
46.4
45.3
89.8
90.1
30.4
30.1
33.8
33.4
23.9
26.5
210
219
211
135 *
130
150
47.0
51.0
11.2
13.5 #
0.82
0.75
6.75
5.80
22.9
24.2
31.0 *
31.9
48.3 *
40.3
98.5
97.6
0.57
0.45

569
1100
9.6
8.5
0.2
0.3
0.7
0.6
1.3
0.9
0.4
0.4
0.3
0.3
1.3
1.6
9
11
44
14
10
9
3.0
2.5
0.6
0.9
0.02
0.04
0.32
0.37
1.7
3.2
3.4
7.3
6.0
9.3
2.5
2.8
0.07
0.06

Week 8
Mean
SEM
55.3
2.1
52.8
2.3
20.5
2.4
15.7
2.7
78.9
3.9
71.4
4.5
25.4
1.8
20.8
2.6
27.1
1.2
24.0
1.4
118
5.4
119
6.0
72
3.6
77
5.1
69.6
3.1
71.2
2.8
6775
557
7121
416
520
8.1
506
6.6
15.8
0.2
15.3
0.3
46.9
0.7
45.4
0.5
90.4
1.4
89.8
0.9
30.5
0.4
30.3
0.5
33.8
0.3
33.7
0.4
24.7 *
1.4
26.5
1.4
210
9
218
11
148
18
174
18
140
10
147
9
47.9
2.7
48.1
2.8
13.0
0.7
13.0
0.8
0.83
0.03
0.76
0.04
6.61
0.29
6.00
0.43
23.7
2.0
21.0
1.7
30.0 **
3.8
25.8
4.6
49.5
6.8
32.8
5.2
94.5
2.4
96.0
2.7
0.59
0.05
0.58
0.06

− 49 −OH

HO O COHOOCH 2 O H

HOHHO H 2 C
O
HO
O
OH
H
HO

O
CH 3

O

H
OH O H

Relative renin activity (%)

Fig. 9 Chemical structure ofsoyasaponin I.

100
80
60
40
20
0
1

100
10
Concentration of soybean saponin(μg/ mL)

1000

Fig. 10 Inhibition of renin activity by soybean
saponin. Relative activity is shown as a percentageof renin activity without soybean saponin. Values
are the mean of triplicate analysis.

− 50 −

Systolic blood pressure (mmHg)

260
control groupsaponin group

240
220

**

*

2
4
6
Experimental period (weeks)

8

200
180
0

Fig. 11 Effect of oral soybean saponin
administration on change in systolic blood pressurein SHR. Symbols indicate control group (opencircle) and saponin group (closed square). Values
are means, and standard errors are depicted byvertical bars (9 rats per group). Mean values weresignificantly different from those of the control
group: *P < 0.05; **P < 0.01.

Table 4 Effect of oral soybean saponin administration on the tissue weight
and the plasma parameters in SHR.
Control
Mean
Tissue weight
Heart

SEM

Soybean saponin
Mean
SEM

(g)
1.43
0.03
1.47
0.04
(g/ 100g BW)
0.42
0.01
0.43
0.01
Liver
(g)
10.83
0.17
10.92
0.12
(g/ 100g BW)
3.18
0.03
3.22
0.02
Kidney (left) (g)
1.46
0.03
1.40
0.03
(g/ 100g BW)
0.43
0.01
0.41
0.01
Kidney (right) (g)
1.34
0.02
1.35
0.02
(g/ 100g BW)
0.40
0.00
0.40
0.01
Plasma biochemical parametersTG(mg/ dL)
54.0
6.6
52.7
3.6
TC
(mg/ dL)
67.6
3.0
62.1
3.2
HDL-C
(mg/ dL)
38.8
2.5
38.9
2.2
LDL-C
(mg/ dL)
18.0
1.5
12.7 **
1.5
Glucose(mg/ dL)
151.1
4.7
158.4
11.5
AST
(U/ L)
79.9
7.5
54.6 **
2.0
ALT(U/ L)
47.1
2.7
38.6 *
1.7
BUN(mg/ dL)
24.1
0.6
20.6 **
0.8
BW, body weight; TG, triglyceride; TC, total cholesterol; HDL-C, high-density
lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; AST, aspartate
aminotransferase; ALT, alanine aminotransferase; BUN, blood urea nitrogen. Meanvalues with their standard errors for 9 rats per group. Mean values were significantly
different from those of the control: *P < 0.05, **P < 0.01.

− 51 −

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Inhibitory effects of lupeol on 3T3-L1 preadipocyte differentiation., Phytochem. Lett., 1,
191-194 (2008)
3) Itoh, M., Hiwatashi, K., Abe, Y., Kimura, F., Toshima, G., Takahashi, J., Sasaki, H., andHata, K., Lupeol reduces triglyceride and cholesterol synthesis in human hepatoma cells.,
Phytochem. Lett., 2, 176-178 (2009)4) Watanabe, T., Hata, K., Hiwatashi, K., Hori, K., Suzuki, N., and Itoh, H., Suppression ofmurine preadipocyte differentiation and reduction of visceral fat accumulation by a
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9) Mori, K., Inatomi, S., Ouchi, K., Azumi, Y., and Tuchida, T., Improving effects of themushroom Yamabushitake (Hericium erinaceus) on mild cognitive impairment: adouble-blind placebo-controlled clinical trial., Phytother. Res., 23, 367-372 (2009)10) Evans, R. M., Barish, G. D., and Wang, Y.-X., PPARs and the complex journey to obesity.,Nat. Med., 10, 355-361 (2004)
11) Takahashi, S., Hori, K., Shinbo, M., Hiwatashi, K., Gotoh, T., Yamada, S., Isolation ofhuman renin inhibitor from soybean: soyasaponin I is the novel human renin inhibitor insoybean., Biosci. Biotechnol. Biochem., 72, 3232-3236 (2008)12) Persson, P. B., Renin: origin, secretion and synthesis., J. Physiol., 552, 667-671 (2003)
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㧠㧚․⸵ߩ᭎ⷐ
1⊒᣿ߩฬ⒓㧦ࠦ࡜ࠥ࠽࡯࠯㒖ኂ૞↪ࠍ᦭ߔࠆഃ்ᡷༀ೷߅ࠃ߮
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౏㐿ᣣ㧦2010 ᐕ 9 ᦬ 9 ᣣ2⊒᣿ߩฬ⒓㧦ᣂⷙ㉂Უ߅ࠃ߮ߘࠇࠍ↪޿ߚ㘶ᢱ࡮㘩ຠ࡮ኅ⇓㘺ᢱ⊒᣿⠪㧦ㅴ⮮ ᣽౏㐿⇟ภ㧦․㐿 2010-284081
౏㐿ᣣ㧦2010 ᐕ 12 ᦬ 24 ᣣ

⊒᣿ߩฬ⒓㧦⢽⾰ઍ⻢ଦㅴ೷‫ޔ‬⢽⾰ઍ⻢㑐ㅪㆮવሶ⊒⃻Ⴧᒝ೷߅ࠃ߮ߘߩ⵾ㅧᣇᴺ
⊒᣿⠪㧦ᮘᷰ৻ਯ‫⇗ޔ‬ᕺม      ዊဈ㕏ᐘ‫ޔ‬ะጊବਯ‫ޔ‬ဈᧄ⾫ੑ㧔ᩣᑼળ␠ဈᧄࡃࠗࠝ㧕౏㐿⇟ภ㧦․㐿 2010-111586
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⊒᣿ߩฬ⒓㧦ฎઍ☨Ớ❗♧ᶧߥࠄ߮ߦߘߩ⵾ᴺ
⊒᣿⠪㧦㊄ሶ 㓉ብ౏㐿⇟ภ㧦․㐿 
౏㐿ᣣ㧦 ᐕ  ᦬  ᣣ⊒᣿ߩฬ⒓㧦ࡂ࠲ࡂ࠲ෆᎽ↱᧪ߩ☼⾰‛‫ߩߘޔ‬ขᓧᣇᴺ߅ࠃ߮↪ㅜ⊒᣿⠪㧦ᚭᨑ৻༑‫ޔ‬Ⴆᧄ⎇৻‫ޔ‬㜞ᯅᔀ‫᧖ޔ‬ጊ⑲᮸‫ᧁ⦁ޔ‬ീ․⸵⇟ภ㧦․⸵╙㧠㧡㧞㧠㧠㧢㧣⊓㍳ᣣ㧦ᐔᚑ㧞㧞ᐕ㧢᦬㧝㧝ᣣ⊒᣿ߩฬ⒓㧦ᣂⷙ㉂Უ෸߮ߘࠇࠍ↪޿ߚᷡ㈬ߩ⵾ㅧᣇᴺ
⊒᣿⠪㧦ᷰㄝ⺈ⴡ‫ޔ‬ᣂ㊁⪲ሶ‫ޔ‬ਛ↰ஜ⟤․⸵⇟ภ㧦․⸵╙㧠㧠㧥㧝㧡㧢㧟⊓㍳ᣣ㧦ᐔᚑ㧞㧞ᐕ㧠᦬㧝㧢ᣣ

1 ⊒᣿ߩฬ⒓㧦ࠦ࡜ࠥ࠽࡯࠯㒖ኂ૞↪ࠍ᦭ߔࠆഃ்ᡷༀ೷߅ࠃ߮
ẩ≌ᒻᚑᡷༀ೷
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౏㐿ᣣ㧦2010 ᐕ 9 ᦬ 9 ᣣ‫ޤ⚂ⷐޣ‬[⺖㗴] ᓥ᧪ࠃࠅ㘩ຠ߽ߒߊߪ㘩ຠ⚛᧚ߣߒߡ೑↪ߐࠇߡ߅ࠅ‫ޔ‬቟ోᕈߦ໧㗴߇ߥ޿ㄘ᳓↥‛ߦ↱᧪ߔࠆᚑಽࠍ᦭ലᚑಽߣߔࠆࠦ࡜ࠥ࠽࡯࠯㒖ኂ೷ࠍឭଏߔࠆ
[⸃᳿ᚻᲑ]
 ࡎ࠶ࡊߩ᛽಴‛ࠍ᦭ലᚑಽߣߒߡ฽᦭ߒߡߥࠆ‫࠯࡯࠽ࠥ࡜ࠦޔ‬㒖ኂ೷‫࡜ࠦޔ‬ࠥ࠽࡯࠯㒖ኂ೷ࠍ฽᦭ߔࠆߎߣࠍ․ᓽߣߔࠆ㘩ຠ߽ߒߊߪ㘩ຠ⚛᧚‫ޔ‬ਗ߮ߦࡆ࡯࡞߹ߚߪ⊒ᵃ㈬ࠍ⵾ㅧߔࠆߦ޽ߚࠅ‫↪૶ࡊ࠶ࡎޔ‬ᢱߩ 50㨪90%ࠍ㤈᳝ߦ‫ߡޟ‬ࠎ߆ߒߒߡᾚᴣߒߡᓧߚ㜞᷷ߩ⹥㤈᳝ߦᱷࠅߩࡎ࠶ࡊࠍട߃ߡ㕒⟎ߒ‫ޔ‬ỹㆊߒߚᓟ‫⊒ޔ‬㉂‫ޔ‬ᾫᚑߔࠆߎߣࠍ․ᓽߣߔࠆ‫࠯࡯࠽ࠥ࡜ࠦޔ‬㒖ኂᵴᕈࠍ᦭ߔࠆࡆ࡯
࡞߹ߚߪ⊒ᵃ㈬ߩ⵾ㅧᣇᴺ

2 ⊒᣿ߩฬ⒓㧦ᣂⷙ㉂Უ߅ࠃ߮ߘࠇࠍ↪޿ߚ㘶ᢱ࡮㘩ຠ࡮ኅ⇓㘺ᢱ⊒᣿⠪㧦ㅴ⮮ ᣽౏㐿⇟ภ㧦․㐿 2010-284081
౏㐿ᣣ㧦2010 ᐕ 12 ᦬ 24 ᣣ‫ޤ⚂ⷐޣ‬[⺖㗴] 㧞㧜㧑ࠛ࠲ࡁ࡯࡞ሽ࿷ਅߢ↢⢒น⢻ߢ޽ࠅ߆ߟࠛ࠲ࡁ࡯࡞↢ᚑ⢻߅ࠃ߮㜞⚖ࠕ࡞ࠦ࡯࡞ࠍᄙߊ↢ᚑߔࠆᣂⷙ㉂Უ߅ࠃ߮ߘࠇࠍ↪޿ߚ㘶ᢱ࡮㘩ຠ‫ޕ‬ኅ⇓㘺ᢱߩឭଏ[⸃᳿ᚻᲑ] ‫ࠄߊߐޟ‬㉂Უ㧝㧠㧠㧥‫ޔߒ᧪↱ߦޠ‬㧞㧜㧑ࠛ࠲ࡁ࡯࡞ሽ࿷ਅߢ↢⢒น⢻ߢ޽ࠅ‫ⷫߟ߆ޔ‬ᩣࠃࠅࠛ࠲ࡁ࡯࡞↢↥⢻߇㜞ߊ㜞⚖ࠕ࡞ࠦ࡯࡞ࠍᄙߊ↢ᚑߔࠆ Sakura-2 NITE P-756 ᩣࠍ↪޿ࠆߎߣࠍ․ᓽߣߔࠆ㘶ᢱ࡮㘩ຠ࡮ኅ⇓㘺ᢱߩ⵾ㅧᴺ

− 55 −

3⊒᣿ߩฬ⒓㧦⢽⾰ઍ⻢ଦㅴ೷‫ޔ‬⢽⾰ઍ⻢㑐ㅪㆮવሶ⊒⃻Ⴧᒝ೷‫ߘ߮ࠃ߅ޔ‬ߩ⵾ㅧᣇᴺ⊒᣿⠪㧦ᮘᷰ৻ਯ‫⇗ޔ‬ᕺม      ዊဈ㕏ᐘ‫ޔ‬ะጊବਯ‫ޔ‬ဈᧄ⾫ੑ㧔ᩣᑼળ␠ဈᧄࡃࠗࠝ㧕౏㐿⇟ภ㧦․㐿 2010-111586
౏㐿ᣣ㧦2010 ᐕ 5 ᦬ 20 ᣣ‫ޤ⚂ⷐޣ‬
㨇⺖㗴㨉↢ᵴ⠌ᘠ∛ߦߟߥ߇ࠆ૕⢽⢌ߩㆊᐲߥ⫾Ⓧࠍᛥ೙ߒ‫ޔ‬ఝࠇߚ⢽⾰ઍ⻢ଦㅴ૞↪‫ޔ‬⢽⾰ઍ⻢㑐ㅪㆮવሶჇᒝ૞↪ࠍ᦭ߔࠆ‛⾰෸߮ߎࠇࠍ฽᦭ߔࠆ⚵ᚑ‛ࠍឭଏߔࠆߎߣ‫ޕ‬㨇⸃᳿ᚻᲑ㨉ࡗࡑࡉࠪ࠲ࠤ෸߮ࡗࡑࡉࠪ࠲ࠤߩ᦭ᯏṁᇦ╬ߦࠃࠆ᛽಴‛‫ޔ‬ਗ߮ߦߎࠇࠍ฽᦭ߔࠆ㘩ຠ‫ޔ‬㘶ᢱ‫ޔ‬ක⮎↪⵾೷෸߮േ‛↪㘺ᢱߢ޽ࠆ⚵ᚑ‛‫ޕ‬⊒᣿ߩฬ⒓㧦ฎઍ☨Ớ❗♧ᶧߥࠄ߮ߦߘߩ⵾ᴺ
⊒᣿⠪㧦㊄ሶ 㓉ብ౏㐿⇟ภ㧦․㐿 
౏㐿ᣣ㧦 ᐕ  ᦬  ᣣ‫ޤ⚂ⷐޣ‬=⺖㗴? ᣂⷙߥේᢱố☳ࠍ૶↪ߒߡ‫࡝ࡐߩߤߥࡦ࠾ࡦ࠲ߪ޿ࠆ޽ࡦࠕࠪ࠻ࡦࠕ⚛⦡ޔ‬ࡈࠚࡁ࡯࡞ࠍ฽ߺ‫ߦ⺞⦡ޔ‬ఝࠇ‫ޔ‬ஜᐽߦࠃ޿↞๧ᢱࠍឭଏߔࠆߎߣ‫ޕ‬=⸃᳿ᚻᲑ? ฎ᧪ߦᣣᧄߢᩱၭߐࠇߡ޿ߚߣߐࠇࠆ‫ޟ‬ฎઍ☨‫ࠍࠇߎޔߒ⋡⌕ߦޠ‬ේᢱߣߒߡ♧ൻߐߖߚฎઍ☨Ớ❗♧ᶧࠍ㐿⊒ߒߚ‫ޔࠅࠃߦࠇߎޕ‬᛫㉄ൻ૞↪߇ᦼᓙߐࠇ
ࠆ⦡⚛ࠕࡦ࠻ࠪࠕࡦ޽ࠆ޿ߪ࠲ࡦ࠾ࡦߥߤߩࡐ࡝ࡈࠚࡁ࡯࡞ࠍ฽ߺ‫⺞⦡ߚ߹ޔ‬߽⦟ᅢߢ‫ޔ‬ᚑಽ਄㕖Ᏹߦஜᐽ⊛ߢ⷗ߚ⋡ߩ⦡ว޿ߦ߽ఝࠇߡ޿ࠆ᳓㘸⁁ߩᣂⷙ↞๧⚛᧚ࠍឭଏߢ߈ࠆ‫ޕ‬− 56 −

5 ⊒᣿ߩฬ⒓ ࡂ࠲ࡂ࠲ෆᎽ↱᧪ߩ☼⾰‛‫ߩߘޔ‬ขᓧᣇᴺ߅ࠃ߮↪ㅜ⊒᣿⠪㧦ᚭᨑ৻༑‫ޔ‬Ⴆᧄ⎇৻‫ޔ‬㜞ᯅᔀ‫᧖ޔ‬ጊ⑲᮸‫ᧁ⦁ޔ‬ീ․⸵⇟ภ㧦․⸵╙㧠㧡㧞㧠㧠㧢㧣⊓㍳ᣣ㧦ᐔᚑ㧞㧞ᐕ㧢᦬㧝㧝ᣣ‫ޤ⚂ⷐޣ‬
㨇⺖㗴㨉ࡂ࠲ࡂ࠲ෆᎽ߆ࠄߩ☼⾰‛ߣෆߩಽ㔌ᣇᴺߩ㐿⊒ߣ☼⾰‛߿ෆߩ᦭
ല೑↪ᴺߩ⏕┙ࠍ⋡⊛ߣߒߚ߽ߩߢ޽ࠆ‫ޕ‬㨇⸃᳿ᚻᲑ㨉ߎߩ⊒᣿ߪ‫ޔ‬㓽ࡂ࠲ࡂ࠲߆ࠄขࠅ಴ߒߚෆᎽࠍ᳓‫ޔ‬ᅢ߹ߒߊߪ
᷷᳓ਛߦᶐẃߒߡಣℂߔࠆᣇᴺߦࠃࠅ‫ߣ‛⾰☼ޔ‬ෆߣࠍಽ㔌ߔࠆᣇᴺࠍ⷗
಴ߒߚ‫ޕ‬ขࠅ಴ߒߚෆᎽࠍ㧟㧜㨪㧥㧜͠ߩ᳓ߦ㧝㨪㧢㧜ಽ㑆ᶐẃߒࡂ࠲ࡂ࠲ෆᎽ↱᧪ߩ☼⾰‛ߣෆࠍಽ㔌ߔࠆᣇᴺࠍ⏕┙ߒߚ‫ޕ‬ಽ㔌ߐࠇߚ☼⾰‛߇ઃ⌕ߒߡ޿ߥ޿ᨵࠄ߆ߥࡂ࠲ࡂ࠲ߩෆߪ㝼ෆടᎿຠߣߒߡߩ೑↪߇น⢻ߢ޽ࠆ‫ޔߚ߹ޕ‬ಽ㔌ߐࠇߚ☼⾰‛ߪᾲ቟ቯᕈߦఝࠇߚ࠲ࡦࡄࠢ⾰ࠍ฽᦭ߒ㘶㘩‛↪Ⴧ☼೷ߣߒߡߩ೑↪߇น⢻ߢ޽ࠆ‫ޕ‬⊒᣿ߩฬ⒓㧦ᣂⷙ㉂Უ෸߮ߘࠇࠍ↪޿ߚᷡ㈬ߩ⵾ㅧᣇᴺ
⊒᣿⠪㧦ᷰㄝ⺈ⴡ‫ޔ‬ᣂ㊁⪲ሶ‫ޔ‬ਛ↰ஜ⟤․⸵⇟ภ㧦․⸵╙㧠㧠㧥㧝㧡㧢㧟⊓㍳ᣣ㧦ᐔᚑ㧞㧞ᐕ㧠᦬㧝㧢ᣣ‫ޤ⚂ⷐޣ‬ާ⺖㗴ިᷡ㈬ߩ⵾ㅧߦ↪޿ࠆ㉂Უߢ޽ߞߡ‫ޔ‬㧟㧷㧤㧙㧝㧡㧣㧭ᩣߦ↱᧪ߒ‫ޔ‬㧞㧜㧑ࠛ࠲ࡁ࡯࡞ሽ࿷ਅߢ↢⢒น⢻ߢ޽ࠅ‫ⷫߟ߆ޔ‬ᩣ↱᧪ߩࠕ࡞ࠦ࡯࡞⊒㉂⢻ߣ㚅᳇↢ᚑ⢻ࠍ᦭ߒ‫᦭ޔ‬ᯏ㉄⚵ᚑ߇ⷫᩣࠃࠅ㜞޿ᷡ㈬㉂Უ㧔㧷㧹㧭㧸࡯㧟㧡ᩣ㧕ࠍឭଏߔࠆߣ౒ߦ‫ޔ‬ᒰ⹥㉂Უࠍ↪޿ߡ㚅ࠅߣ๧߇⦟ᅢߥᷡ㈬ࠍല₸ࠃߊ⵾ㅧߔࠆᣇᴺ
ࠍឭଏߔࠆߎߣࠍ⋡⊛ߣߒߡ޿ࠆ‫ޕ‬ާ⸃᳿ᚻᲑި
ᷡ㈬㉯ㅧߦ߅޿ߡ‫ⷫޔ‬ᩣ↱᧪ߩ㚅๧ࠍࡃ࡜ࡦࠬࠃߊ↢ᚑߒ‫᦭ޔߟ߆ޔ‬ᯏ㉄⚵ᚑ߇ⷫᩣࠃࠅ㜞޿ᷡ㈬㉂Უ 5CEEJCTQO[EGUEGTGXKUKCG 㧷㧹㧭㧸㧙㧟㧡ᩣ㧔㧺㧵㨀㧱 㧭㧼㧙㧠㧕‫ޔ‬ਗ߮ߦ‫ޔ‬ᒰ⹥㉂Უࠍ૶↪ߔࠆᷡ㈬ߩ⵾ㅧᴺ‫ޕ‬− 57 −

䋵䋮ቇળ⊒⴫ⷐᣦ䋨31 ઙ䋩1) ⊒⴫ቇળ㧦╙ 76 ࿁ᣣᧄ↢ൻቇળ᧲ർᡰㇱᄢળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 5 ᦬ 8 ᣣ‫⑔࠮࠶࡜ࠦޔ‬ፉ㧔⑔ፉᏒ㧕Ṷ㗴ฬ㧦᣸⯻⚦⢩-ࡃࠠࡘࡠ࠙ࠖ࡞ࠬ⊒⃻♽ߦ߅ߌࠆࠕࠬࡄ࡞࠹ࠖ࠶ࠢࡊࡠ࠹ࠕ࡯࠯ ߦߟ޿ߡ
⊒⴫⠪㧦ዊ㊁ᵗノ, ᓟ⮮⁴, ⩵࿾⾫৻ (⑺↰ᄢቇ࡮Ꮏቇ⾗Ḯ),㖴Ỉᖗ (࿖㓙ㄘ⎇࠮),㜞ᯅ⍾❱ (⑺↰⋵✚㘩⎇࠮)2) ⊒⴫ቇળ㧦╙ 16 ࿁⑺↰ᔕ↪↢๮⑼ቇ⎇ⓥળቇⴚ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 5 ᦬ 28 ᣣ‫✚⋵↰⑺ޔ‬ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧔⑺↰Ꮢ㧕Ṷ㗴ฬ㧦Sf-9 ᣸⯻⚦⢩-ࡃࠠࡘࡠ࠙ࠖ࡞ࠬ⊒⃻♽ߦ߅ߌࠆࠕࠬࡄ࡞࠹ࠖ࠶ࠢ
ࡊࡠ࠹ࠕ࡯࠯ߩ↢ᚑ᜼േߣ․ᕈ⸃ᨆ
⊒⴫⠪㧦ዊ㊁ᵗノ, ᓟ⮮⁴, ⩵࿾⾫৻ (⑺↰ᄢቇ࡮Ꮏቇ⾗Ḯ)‫ޔ‬㖴Ỉᖗ (࿖㓙ㄘ⎇࠮)‫ޔ‬㜞ᯅ⍾❱ (⑺↰⋵✚㘩⎇࠮) ⊒⴫ቇળ㧦╙  ࿁⑺↰ᔕ↪↢๮⑼ቇ⎇ⓥળቇⴚ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫✚⋵↰⑺ޔ‬ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧔⑺↰Ꮢ㧕Ṷ㗴ฬ㧦ේᩭᓸ↢‛↱᧪ &ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯㧔2CGPKFCUG㧕ㆮવሶߩࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯߆ࠄߩන㔌⊒⴫⠪㧦㖴Ỉ ᖗ㧔⁛ᴺ࿖㓙ㄘᨋ᳓↥ᬺ⎇ⓥ࠮㧕‫ޔ‬㜞ᯅ⍾❱㧔⑺↰⋵✚㘩⎇࠮㧕4) ⊒⴫ቇળ䋺╙ 㪋㪐 ࿁ᣣᧄ↢૕කᎿቇળᄢળ㩷⊒⴫ᣣ䈫႐ᚲ䋺2010 ᐕ 6 ᦬ 25 ᣣ䇮ᄢ㒋࿖㓙੤ᵹ䉶䊮䉺䊷䋨ᄢ㒋Ꮢ䋩Ṷ㗴ฬ䋺Study on evaluation of food by ERP㩷 P300 in the case while changing thenumber of displayed pictures㩷 corresponding to each opinion⊒⴫⠪䋺Tomohiro Honma*䇮Motoshi Tanaka*䇮Hiroshi Inoue*䇮Yoshitsugu Niiyama*䇮
Takahashi Toru**䇮Masanori Kumagai**䇮Yoshinobu Akiyama***䋨 *Akita University 䇮 **Akita Research Institute for Food and Brewing 䇮 ***Akita
Prefectural University䋩 ⊒⴫ቇળ㧦╙  ࿁ࠠ࠴ࡦ࡮ࠠ࠻ࠨࡦ࡮ࠪࡦࡐࠫ࠙ࡓ
⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫᧲ޔ‬੩ᄢቇ㧔᧲੩ㇺᢥ੩඙㧕Ṷ㗴ฬ㧦ࡃࠠࡘࡠ࠙ࠗ࡞ࠬᗵᨴ᣸⯻⚦⢩ 5H ↱᧪ࠠ࠴࠽࡯࠯ߩ෻ᔕ․ᕈ
⊒⴫⠪㧦᳓ේ㤗⟤ሶ㧘ᓟ⮮ ⁴㧖㧘㜞ᯅ⍾❱㧖㧖㧘ᷓḴ ᘮ㧔ㄭ⇰ᄢ㒮࡮ㄘ࡮ࡃࠗࠝ
㧖
⑺↰ᄢ㒮࡮Ꮏቇ⾗Ḯ㧘㧖㧖⑺↰⋵࡮✚㘩⎇࠮㧕6) ⊒⴫ቇળ䋺╙䋱䋳࿁ᣣᧄశ⣖ᯏ⢻䉟䊜䊷䉳䊮䉫⎇ⓥળ㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ䋷᦬ 㪉㪋 ᣣ䇮ᤊ㒺ળ㙚䋨᧲੩ㇺ䋩㩷Ṷ㗴ฬ䋺㘩ຠ䈱ᄖ༵ⷰᅢ⹏ଔᤨ䈮䈍䈔䉎೨㗡೨㊁ዪᚲ⣖ⴊᵹേᘒ䈱⸃ᨆ㩷⊒⴫⠪䋺䂾ᾢ⼱᣽ೣ䋪䇮㜟ᯅᔀ䋪䇮⑺ጊ⟤ዷ䋪䋪㩷 㩷
䋨䋪⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䇮䋪䋪⑺↰⋵┙ᄢቇ䋩㩷㩷㪎㪀㩷 ⊒⴫ቇળ䋺ᐔᚑ 㪉㪉 ᐕᐲ㔚᳇㑐ଥቇળ᧲ർᡰㇱㅪวᄢળ㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ 㪏 ᦬ 㪉㪍 ᣣ䇮౎ᚭᄢቇ䋨㕍᫪⋵౎ᚭᏒ䋩㩷Ṷ㗴ฬ䋺䉬䊷䉨䉕଀䈫䈚䈢㘩ຠ䈱ᅢ䉂⹏ଔᤨ䈱 㪜㪩㪧 ⸘᷹㩷⊒⴫⠪䋺䂾↰ਛరᔒ䇮ᧄ㑆ᥓᄢ䇮੗਄㩷 ᶈ䇮ᣂጊ༑༹䇮㜞ᯅ㩷 ᔀ㺒䇮㩷 ᾢ⼱᣽ೣ㺒䇮㩷 㩷⑺ጊ⟤ዷ㺒㺒㩷
䋨⑺↰ᄢቇ䇮㺒⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䇮㺒㺒⑺↰⋵┙ᄢቇ䋩㩷㩷㪏㪀㩷 ⊒⴫ቇળ䋺The 23rd Annual and International Meeting of the Japanese Association
for Animal Cell Technology (JAACT2010)⊒⴫ᣣ䈫႐ᚲ䋺2010 ᐕ 9 ᦬ 2 ᣣ䇮ർᶏ㆏ᄢቇ䋨ᧅᏻᏒ䋩Ṷ㗴ฬ䋺Lupeol reduces lipid synthesis in adipocyte and hepatocyte⊒⴫⠪䋺Keishi Hata1 , Junichiro Takahashi2
1
Akita Research Institute for Food & Brewing (ARIF), Akita, 010-1623, Japan,
2
Skylight Biotech. Inc., Akita, 010-0911, Japan㩷9) ቇળ⊒⴫㧦ᣣᧄ㘩ຠ⑼ቇᎿቇળ╙ 57 ࿁ᄢળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 2 ᣣ‫᧲ޔ‬੩ㄘᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦㤕⩶ᩣ࡜ࠗࡉ࡜࡝࡯ᵴ↪ߦࠃࠆ☨๧ཬߩࠗ࠰ࡑ࡞࠻࡯ࠬჇടലᨐ
⊒⴫⠪㧦ᷰㄝ㓉ᐘ‫ޔ‬૒⮮ᕺ㉿‫ޔ‬૒‫ᧁޘ‬ᐽሶ㧔⑺↰⋵✚㘩⎇࠮㧕‫ޔ‬૒⮮ീ‫ޔ‬ⅶ↢៨‫੹ޔ‬㊁ብ㧔㧔ᩣ㧕⑺↰੹㊁໡ᐫ㧕10) ⊒⴫ቇળ㧦╙ 57 ࿁ᣣᧄ㘩ຠ⑼ቇᎿቇળᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 2 ᣣ‫᧲ޔ‬੩ㄘᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦࡟࠾ࡦᵴᕈߦ෸߷ߔࠨࡐ࠾ࡦ㘃ߩᓇ㗀ߦߟ޿ߡ㧦᭴ㅧᯏ⢻⋧㑐⸃ᨆ⊒⴫⠪㧦㜞ᯅ⍾❱‫ޔ‬ၳ ৻ਯ‫⟤⧛଻ޔ‬૫‫ޔ‬ᓟ⮮ ⁴㧝
㧔⑺↰⋵✚㘩⎇࠮‫ޔ‬㧝⑺↰ᄢቇᎿቇ⾗Ḯ㧕11) ⊒⴫ቇળ㧦ᣣᧄ㘩ຠ⑼ቇᎿቇળ╙ 57 ࿁ᄢળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 2 ᣣ‫᧲ޔ‬੩ㄘᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦ૐࠣ࡞࠹࡝ࡦ☨ߩ․ᓽߦߟ޿ߡ
⊒⴫⠪㧦ᄢ⢻ବਭ‫ޔ‬ᚭ᧻⺈‫ޔ‬㜞⇗⡡‫ޔ‬Ⴆᧄ⎇৻㧔⑺↰⋵✚㘩⎇࠮㧕12) ⊒⴫ቇળ㧦╙ 10 ࿁㘩ຠ㉂⚛ൻቇ⎇ⓥળቇⴚ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 4 ᣣ‫ޔ‬ᄢ㒋ᐭ┙ᄢቇ㧔ᄢ㒋ᐭ႓Ꮢ㧕Ṷ㗴ฬ㧦ࡃࠠࡘࡠ࠙ࠗ࡞ࠬ࡮᣸⯻⚦⢩ߦ߅ߌࠆࡅ࠻࡟࠾ࡦߩല₸⊛↢↥ߣߘࠇࠍ↪޿ߚ㒖ኂ‛⾰ត⚝ߦߟ޿ߡ⊒⴫⠪㧦㜞ᯅ⍾❱㧝‫ޔ‬ၳ ৻ਯ㧝㧘቟๺ᐢਫ㧞‫⩵ޔ‬࿾⾫৻㧞‫ޔ‬ᓟ⮮ ⁴㧞
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞⑺↰ᄢ࡮Ꮏቇ⾗Ḯ㧕13) ⊒⴫ቇળ䋺↢૕කᎿቇ䉲䊮䊘䉳䉡䊛 㪉㪇㪈㪇㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ 㪐 ᦬ 㪈㪇 ᣣ䇮ർᶏ㆏ᄢቇ㩷Ṷ㗴ฬ䋺㪜㪩㪧 䉕ᜰᮡ䈫䈚䈢㘩ຠ⹏ଔ䈮㑐䈜䉎ታ㛎⊛ᬌ⸛㩷 㵩㩷 ↉䉕଀䈫䈚䈢ᄖⷰ⹏ଔ㵩㩷⊒⴫⠪䋺䂾↰ਛరᔒ㵴㩷 䇮ᧄ㑆ᥓᄢ㵴䇮㩷 ੗਄㩷 ᶈ㵴㩷 䇮ᣂጊ༑༹㵵䇮㩷 㜞ᯅ㩷 ᔀ㺒䇮㩷 㩷ᾢ⼱᣽ೣ㺒䇮㩷 ⑺ጊ⟤ዷ㺒㺒㩷㩿㵴⑺↰ᄢቇᄢቇ㒮Ꮏቇ⾗Ḯቇ⎇ⓥ⑼䇮㵵⑺↰ᄢᄢቇ㒮කቇ♽⎇ⓥ⑼䇮㺒⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷㺒㺒⑺↰⋵┙ᄢቇ↢‛⾗Ḯ⑼ቇㇱ㪀㩷㩷 ⊒⴫ቇળ㧦 ᣣᧄᗵᕈᎿቇળᐕᰴᄢળ
⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ ᧲੩Ꮏᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦࿾ၞ․↥ຠ㐿⊒ߦ߅ߌࠆᗵᕈࡑ࡯ࠤ࠹ࠖࡦࠣᚻᴺߩ೑↪ߩᬌ⸛⊒⴫⠪㧦٤㜞⇗ ⡡ 㧔⑺↰⋵✚㘩⎇࠮㧕15) ⊒⴫ቇળ䋺╙ 㪌㪎 ࿁ᣣᧄᩕ㙃ᡷༀቇળቇⴚ✚ળ㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ 㪐 ᦬ 㪈㪈 ᣣ䇮ᅚሶᩕ㙃ᄢቇ䋨ၯ₹⋵ဈᚭᏒ䋩㩷Ṷ㗴ฬ䋺䉺䊮䊌䉪⾰ടᎿ㘩ຠ䈏᭽䇱䈭ṁᇦ䈱๧⾰䈮෸䈿䈜ᓇ㗀䈮㑐䈜䉎ቴⷰ⊛⹏ଔ㩷⊒⴫⠪䋺䂾↰┵㩷 ብల䋱䋩䇮ᾢ⼱㩷 ᣽ೣ䋲䋩䇮ศ↰㩷 ᥓ䋳䋩䇮㋈ᧁ㩷 㕏ᔒ䋱䋩㩷䋨䋱䋩䉰䊤䊟ᩣᑼળ␠㩷 䊋䉟䉥䉬䊚䉦䊦⎇ⓥᚲ䇮䋲䋩⑺↰⋵✚㘩⎇䉶䋳䋩䇮᧲੩䉰䊤䊟ᩣᑼળ␠㩷ᩕ㙃䉬䉝ផㅴቶ䋩㩷㩷16) ⊒⴫ቇળ㧦ᣣᧄᔕ↪♧⾰⑼ቇળᐔᚑ 22 ᐕᐲᄢળ㧔╙ 59 ࿁㧕⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 15 ᣣ‫ࡊ࠶ࠪࡦ࡜ࠣޔ‬㧔㕒ጟᏒ㧕Ṷ㗴ฬ㧦㧦ੑ㊀ᄌ⇣૕☨⢦੃ố☳ߩ‛ℂൻቇ․ᕈ
⊒⴫⠪㧦㜟ᯅᔀ 1, ቟᧲┥৻ 2, ⧎ၔൟ 3, ᾢ⼱᣽ೣ 1, ਛ᧛଻ౖ 4, ⮮↰⋥ሶ 4 (⑺↰✚㘩⎇࠮ 1, ᣣᧄ㘩ຠൻᎿ 2, 㣮ఽፉᄢ࡮ㄘ 3, ⑺↰⋵┙ᄢ 4)17) ⊒⴫ቇળ㧦╙ 6 ࿁ D-ࠕࡒࡁ㉄⎇ⓥળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 17 ᣣ‫ޔ‬ንጊ࿖㓙ળ⼏႐㧔ንጊᏒ㧕Ṷ㗴ฬ㧦ේᩭᓸ↢‛↱᧪ D-ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯㧔Paenidase㧕ㆮવሶߩࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯߆ࠄߩන㔌‫ޔ‬෸߮↢↥⩶ߩోࠥࡁࡓࠪ࡯ࠢࠛࡦࠬ
⊒⴫⠪㧦㖴Ỉᖗ㧝㧘㜞ᯅ⍾❱㧞㧔㧝࿖㓙ㄘ᳓⎇ⓥ࠮‫ޔ‬㧞⑺↰⋵✚㘩⎇࠮㧕18) ⊒⴫ቇળ㧦╙ 6 ࿁ D-ࠕࡒࡁ㉄⎇ⓥળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 17 ᣣ‫ޔ‬ንጊ࿖㓙ળ⼏႐㧔ንጊᏒ㧕Ṷ㗴ฬ㧦⢖⢩਄⊹⚦⢩ߦ߅ߌࠆ Protein L-isoaspartyl/D-aspartyl methyltransferase ㆮવሶࡁ࠶ࠢ࠳࠙ࡦߪ epithelial-to-mesenchymal transition (EMT)ࠍ⺃ዉߔࠆ⊒⴫⠪㧦ዊ═ේᱜੱ㧝㧘ᚭ↰ᐕ✚㧞‫ޔ‬㊀ᧄ๺ብ㧞‫ޔ‬㖴Ỉᖗ㧟‫ޔ‬㜞ᯅ⍾❱㧠‫ޔ‬ጊౝᐢᐔ㧡㧔㧝ᗲᇫᄢቇ࡮ක‫ޔ‬㧞᧲੩ㇺஜᐽ㐳ኼක≮࠮ࡦ࠲࡯‫ޔ‬㧟࿖㓙ㄘᨋ᳓↥ᬺ⎇ⓥ࠮‫ޔ‬㧠⑺↰⋵✚㘩⎇࠮‫ޔ‬㧡ጤᚻකᄢ࡮ක㧕19) ቇળ⊒⴫㧦 ᐕᐲᣣᧄᯏળቇળ᧲ർᡰㇱ╙ 46 ᦼ⑺ቄ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫↰⑺ޔ‬ᄢቇ㧔⑺↰Ꮢ㧕Ṷ㗴ฬ㧦ੇᑼᓸ☳⎈ߒߚ᧖᧚ߩ㉂⚛♧ᄌ឵࡮ࠛ࠲ࡁ࡯࡞ᄌ឵ߩ⎇ⓥ⊒⴫⠪㧦᪢Ỉ ବ╷‫ޔ‬ዊᨋ ᷕ৻‫ޔ‬㜞ᯅ ᱞᒾ‫ޔ‬દ⮮ ৻ᔒ‫ޔ‬દ⮮ ᣂ㧔⑺↰⋵┙ᄢቇ㧕ㅴ⮮ ᣽ ⑺↰⋵✚㘩⎇࠮ 20) ⊒⴫ቇળ㧦╙  ࿁ᣣᧄ↢‛Ꮏቇળᄢળ
⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫࠻࠶ࡒࠨ࡯࠲ࡦ࠮ࡦ࡚ࠪࡦࡌࡦࠦ࠼࡞࡯ࡢޔ‬㧔ችፒ⋵ችፒᏒ㧕Ṷ㗴ฬ㧦ᷡ㈬ߩ๒๧ߦ㑐ଥߔࠆࠕࡒࡁ㉄ઍ⻢↥‛ߦ෸߷ߔࡈࠖ࠴ࡦ㉄ߩᓇ㗀⊒⴫⠪㧦٤㜞ᯅ ੳ㧔⑺↰✚㘩⎇࠮㧕‫ޔ‬૒‫౏ᧁޘ‬ᄥ‫ޔ‬દ⮮ବᒾ‫ޔ‬ጤ㊁ำᄦ㧔⑺↰⋵ᄢ㧕21) ⊒⴫ቇળ㧦╙㧤࿁♻⁁⩶ಽሶ↢‛ቇࠦࡦࡈࠔ࡟ࡦࠬ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 11 ᦬ 18 ᣣ‫ޔ‬ᐢፉᄢቇ㧔᧲ᐢፉᏒ㧕Ṷ㗴ฬ㧦ࠬ࠻࡟ࠬᔕ╵ mRNA ᄌേ⸃ᨆߦࠃࠆ㤕⩶㧔Aspergillus oryzae㧕ߩᯏ⢻⊛ᄖ᧪ᕈㆮવሶត⚝⊒⴫⠪㧦ዊ═ේඳା㧝‫ޔ‬㜞ᯅ⍾❱㧝‫ޔ‬੖๧ൎ਽㧞
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞᧲ർᄢ㒮ㄘ࡮↢‛↥ᬺഃᚑ㧕22) ⊒⴫ቇળ㧦╙ 83 ࿁ᣣᧄ↢ൻቇળ࡮╙ 33 ࿁ᣣᧄಽሶ↢‛ቇળวหᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 12 ᦬ 7 ᣣ‫␹ޔ‬ᚭࡐ࡯࠻ࠕࠗ࡜ࡦ࠼㧔␹ᚭᏒ㧕Ṷ㗴ฬ㧦࡟࠾ࡦ㒖ኂࠨࡐ࠾ࡦ㧦᭴ㅧ࡮ᯏ⢻⋧㑐⸃ᨆ⊒⴫⠪㧦㜞ᯅ⍾❱‫ޔ‬ၳ ৻ਯ‫ޔ‬ᓟ⮮ ⁴*‫᧖ޔ‬ጊବඳ**(⑺↰⋵✚㘩⎇࠮‫↰⑺*ޔ‬ᄢቇ࡮Ꮏቇ⾗Ḯ‫↰⑺**ޔ‬ᄢቇ࡮කቇㇱ)23) ⊒⴫ቇળ㧦╙ 83 ࿁ᣣᧄ↢ൻቇળ࡮╙ 33 ࿁ᣣᧄಽሶ↢‛ቇળวหᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 12 ᦬ 7 ᣣ‫␹ޔ‬ᚭࡐ࡯࠻ࠕࠗ࡜ࡦ࠼㧔␹ᚭᏒ㧕Ṷ㗴ฬ㧦ේᩭᓸ↢‛↱᧪ D -ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯㧔Paenidase㧕ㆮવሶߩࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯߆ࠄߩන㔌⊒⴫⠪㧦㖴Ỉ ᖗ㧔⁛ᴺ࿖㓙ㄘᨋ᳓↥ᬺ⎇ⓥ࠮㧕‫ޔ‬㜞ᯅ⍾❱㧔⑺↰⋵✚㘩⎇࠮㧕      24) ⊒⴫ቇળ㧦╙ 83 ࿁ᣣᧄ↢ൻቇળ࡮╙ 33 ࿁ᣣᧄಽሶ↢‛ቇળวหᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 12 ᦬ 7 ᣣ‫␹ޔ‬ᚭࡐ࡯࠻ࠕࠗ࡜ࡦ࠼㧔␹ᚭᏒ㧕Ṷ㗴ฬ㧦Protein isoasparatyl methyltransferase gene knock down induced human alveolar
epithelial to mesenchymal cell transition through endoplasmic reticulum stress⊒⴫⠪㧦ዊ═ේ ᱜੱ 1㧘ᚭ↰ ᐕ✚ 2㧘㖴Ỉ ᖗ 3㧘೨ጊ ৻㓉 1㧘㜞ᯅ ⍾❱ 4㧘ጊౝ ᐢᐔ 5㧝ᗲᇫᄢ࡮㒮කቇ♽⎇ⓥ⑼࡮⮎ℂቇ㧘2 ᧲੩ㇺ⠧ੱ✚ว⎇ⓥᚲ㧘3࿖㓙ㄘᨋ᳓↥⎇ⓥ࠮㧘4 ⑺↰⋵✚㘩⎇࠮㧘5 ጤᚻක⑼ᄢቇ࡮ౝ⑼25) ቇળ⊒⴫㧦ᣣᧄࠛࡀ࡞ࠡ࡯ቇળ ╙㧢࿁ࡃࠗࠝࡑࠬ⑼ቇળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 1 ᦬ 13 ᣣ‫ޔ‬ᄢ㒋ᄢቇ㧔็↰Ꮢ㧕Ṷ㗴ฬ㧦࠮࡞ࡠ࡯ࠬ♽ࡃࠗࠝࡑࠬ߆ࠄߩ㧞Ბ㓏⊒㉂ᴺߦࠃࠆࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥⊒⴫⠪㧦ㅴ⮮ ᣽‫↰⷏ޔ‬ቁિ (⑺↰⋵✚㘩⎇࠮)26) ⊒⴫ቇળ㧦The 8th International Aspergillus Meeting (Asperfest8)
The 26th Fungal Genetics Conference (FGC26)⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 14 ᣣ‫ޔ‬16 ᣣ‫ޔ‬Asilomar Conference Center
㧔Asilomar, Pacific Grove, CA, USA)Ṷ㗴ฬ㧦Exploratory survey for potential transposable elements in
Aspergillus oryzae by a stress-fluctuation cDNA browser.
⊒⴫⠪㧦Hironobu Ogasawara1, Saori Takahashi1, and Katsuya Gomi2
1
Akita Res. Inst. Food and Brewing,2
Graduate School of Agricultural Science, Tohoku University27) ⊒⴫ቇળ㧦ᣣᧄㄘ⧓ൻቇળ 2011 ᐕᐲᄢળ⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 26 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦mRNA ᄌേ⸃ᨆߦࠃࠆ㤕⩶㧔Aspergillus oryzae㧕ߩࠬ࠻࡟ࠬᔕ╵ㆮવሶߩត⚝⊒⴫⠪㧦ዊ═ේඳା㧝‫ޔ‬㜞ᯅ⍾❱㧝‫ޔ‬੖๧ൎ਽㧞
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞᧲ർᄢ㒮ㄘ࡮↢‛↥ᬺഃᚑ㧕28) ቇળ⊒⴫㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 26 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦࠮࡞࡜࡯࠯㜞⊒⃻ Pichia pastoris ࠍ↪޿ߚ㧯㧮㧼ߦࠃࠆ࠮࡞ࡠ࡯ࠬ߆ࠄߩ
⋥ធࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥⊒⴫⠪㧦ㅴ⮮ ᣽‫ ᧄ┻ޔ‬ᶈ‫ޔ‬Ⴧ↰␽ሶ‫ޔ‬㜞ᯅఝ᢯‫ޔ‬ਣጊ㆏ሶ 1‫ޔ‬੖ච፲࿻ᣣሶ 1‫ޔ‬㞯ፉᱜᶈ 1㧔⑺↰⋵✚㘩⎇࠮‫ޔ‬1 ᧲ᄢ㒮ㄘ↢⑼㧕
29) ቇળ⊒⴫㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 27 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦ࡔࠗ࡜࡯࠼෻ᔕ↢ᚑ‛ߦࠃࠆࠕ࡞ࠦ࡯࡞⊒㉂㒖ኂᯏ᭴ߩᬌ⸽
⊒⴫⠪㧦⷏↰ ቁિ 1‫ޔ‬ᚭ᧻ ߐ߿߆ 1‫ޔ‬૒‫⟤ ᧁޘ‬Ꮧሶ 1‫ޔ‬ਃᯅ ⑲৻ 2‫ޔ‬ᨋ ␞ሶ 2‫ޔ‬ㅴ⮮ ᣽ 1㧔1 ⑺↰✚㘩⎇࠮‫ޔ‬2 ࡃࠗࠝࠛ࠲ࡁ࡯࡞⎇ⓥ⚵ว㧕30) ቇળ⊒⴫㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 27 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦ࡃࠗࠝࡑࠬ♧ൻᶧߦ߅ߌࠆࠕ࡞ࠦ࡯࡞⊒㉂㒖ኂᯏ᭴ߩ⸃᣿
⊒⴫⠪㧦⷏↰ ቁિ 1‫ޔ‬ᚭ᧻ ߐ߿߆ 1‫ޔ‬૒‫⟤ ᧁޘ‬Ꮧሶ 1‫ޔ‬਄᧛ Პ 2‫ޔ‬ㅴ⮮ ᣽ 1㧔1 ⑺↰✚㘩⎇࠮‫ޔ‬2JXࠛࡀ࡞ࠡ࡯ਛ⎇㧕31) ⊒⴫ቇળ㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇળᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 27 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦☨↱᧪࡟࠾ࡦ㒖ኂ‛⾰ߩหቯߣ᭴ㅧᯏ⢻⋧㑐⸃ᨆ⊒⴫⠪㧦㜞ᯅ⍾❱㧝‫ޔ‬Ᏹ⋚㊁ື↢㧞‫ ⇗ޔ‬ᕺม㧝‫ޔ‬ዊ₹㇚ሶ㧟‫⟤⧛଻ޔ‬૫㧝‫ޔ‬㋈ᧁᄹ✜㧝‫ޔ‬ศỈ⚿ሶ㧞‫ޔ‬ᓟ⮮ ⁴㧠
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞⑺↰⋵┙ᄢቇ࡮↢‛⾗Ḯ‫ޔ‬㧟⑺↰⋵ㄘᨋ᳓↥ᛛ࠮࡮ㄘᬺ⹜㛎႐‫ޔ‬㧠⑺↰ᄢቇᄢቇ㒮࡮Ꮏቇ⾗Ḯ㧕1) ⊒⴫ቇળ㧦╙ 76 ࿁ᣣᧄ↢ൻቇળ᧲ർᡰㇱᄢળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 5 ᦬ 8 ᣣ‫⑔࠮࠶࡜ࠦޔ‬ፉ㧔⑔ፉᏒ㧕Ṷ㗴ฬ㧦᣸⯻⚦⢩-ࡃࠠࡘࡠ࠙ࠖ࡞ࠬ⊒⃻♽ߦ߅ߌࠆࠕࠬࡄ࡞࠹ࠖ࠶ࠢࡊࡠ࠹ࠕ࡯࠯ ߦߟ޿ߡ⊒⴫⠪㧦ዊ㊁ᵗノ, ᓟ⮮⁴, ⩵࿾⾫৻ (⑺↰ᄢቇ࡮Ꮏቇ⾗Ḯ),㖴Ỉᖗ (࿖㓙ㄘ⎇࠮),㜞ᯅ⍾❱ (⑺↰⋵✚㘩⎇࠮)‫ޤ⸒✜ޣ‬᣸⯻⚦⢩-ࡃࠠࡘ࠙ࠖ࡞ࠬ⊒⃻♽ߪ, ᄖ᧪ㆮવሶࠍዉ౉ߒߚࡃࠠࡘࡠ࠙ࠖ࡞ࠬࠍ᣸⯻⚦⢩ߦᗵᨴߐߖࠆߎߣߦࠃࠅ⋡⊛࠲ࡦࡄࠢ⾰ߩ㜞ല₸ߥ↢↥ࠍน⢻ߦߔࠆ‫ߒ߆ߒޕ‬, ᣸⯻⚦⢩߿ࡃࠠࡘࡠ࠙ࠖ࡞ࠬߩㆮવሶߦࠦ࡯࠼ߐࠇߡ޿ࠆౝ࿷ᕈࡊࡠ࠹ࠕ࡯࠯ߦࠃࠅ, ⋡⊛࠲ࡦࡄࠢ⾰ߩ෼㊂ߩૐਅ߇ ᔨߐࠇࠆ‫߼ߚߩߎޕ‬, ല₸⊛ߥ࠲ࡦࡄࠢ⾰↢↥ߩߚ߼ߦ, ߎࠇࠄߩౝ࿷ᕈࡊࡠ࠹ࠕ࡯࠯ߩ⒳㘃߿․ᕈࠍᛠីߒߡ߅ߊߎߣ߇㊀ⷐߣߥࠆ‫⎇ᧄޕ‬ⓥߢߪౝ࿷ᕈࡊࡠ࠹ࠕ࡯࠯ߩ৻ߟߢ޽ࠅ, ࡍࡊࠬ࠲࠴ࡦߦࠃࠅᒝߊᵴᕈ㒖ኂࠍฃߌࠆࡊࡠ࠹ࠕ࡯࠯㧔SAP㧕ߩ․ᕈ⸃ᨆࠍⴕߞߚߩߢႎ๔ߔࠆ‫ޕ‬‫ޣ‬ታ㛎ᣇᴺ‫ޤ‬ᜰᢙᦼߩ Sf-9 ᣸⯻⚦⢩ Ộᶧߦࡅ࠻ࡊ࡟ࡊࡠ࡟࠾ࡦ cDNA ዉ౉ࡃࠠࡘࡠ࠙ࠖ࡞ࠬࠍ
ᗵᨴᄙ㊀ᐲ(MOI) 1 pfu/cells ߢᗵᨴߐߖ, 28͠ߢᠣᜈၭ㙃ࠍ㐿ᆎߒߚ‫ޕ‬ᗵᨴ 6 ᣣ⋡ߩၭ㙃ᶧࠍ࿁෼ߒ, Pepstatin-aminohexyl Sepharose, DEAE-Sepharose FF,߅ࠃ߮ Superdex-200 ࠞ࡜ࡓࠢࡠࡑ࠻ࠣ࡜ࡈࠖ ࡯ ߦ ࠃ ࠅ SAP ߩ ♖ ⵾ ࠍ ⴕ ߞ ߚ ‫ ޕ‬SAP ߩ ᵴ ᕈ ୯ ᷹ ቯ ߦ ߪ Ⱟ శ ⥄ Ꮖ ᶖ శ ၮ ⾰ MOCAc-
Gly-Lys-Pro-Ile-Leu-Phe*Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 (*ᗐቯߐࠇࠆࡍࡊ࠴࠼⚿วಾᢿㇱ૏)ࠍ
↪޿, 37͠ߢ෻ᔕᓟ‫Ⱟޔ‬శᒝᐲ㧔ബ⿠ᵄ㐳 328 nm㧘᷹ቯᵄ㐳 393 nm㧕ࠍ᷹ቯߒߚ‫ޕ‬‫⚿ޣ‬ᨐߣ⠨ኤ‫ޤ‬SAP ߩ⚦⢩ᄖᵴᕈߪ, ᗵᨴၭ㙃ߩ⚻ㆊߦ઻ߞߡࠁࠆ߿߆ߦ਄᣹ߒ, ⚦⢩߇ᱫṌߔࠆၭ㙃 6 ᣣ⋡ߦᄢ߈ߊჇടߒߚ‫ޔߢߎߘޕ‬ᗵᨴ 6 ᣣ⋡ߩၭ㙃ᶧ 200 ml ߆ࠄ SAP ߩ♖⵾ࠍ⹜ߺ, 3 Ბ㓏ߩࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ߢ⚂ 29 Pg ߩ♖⵾ SAP ࠍᓧߚ‫ ⵾♖ޕ‬SAP ߩಽሶ㊂ߪࠥ࡞ỹㆊᴺߦࠃࠅ
37 kDa,߹ߚ SDS-PAGE ߢ 42 kDa ߣ᳞߼ࠄࠇߚ‫ ࠅࠃࠇߎޕ‬SAP ߪන㊂૕ߢሽ࿷ߔࠆߎߣ߇␜ߐࠇ
ߚ‫ޕ‬SAP ߩ N ᧃ┵ 25 ᱷၮߩࠕࡒࡁ㉄㈩೉ࠍ⸃ᨆߒߚ⚿ᨐ, SAP ߪࠞࠗࠦ߿࡚࡚ࠪ࠙ࠫ࠙ࡃࠛ↱᧪ߩࠞ࠹ࡊࠪࡦ D ߣ⋧หᕈ߇⹺߼ࠄࠇߚ‫Ⱟޕ‬శ⥄Ꮖᶖశၮ⾰ࠍ↪޿ߚ႐วߩ SAP ᵴᕈߩ⥋ㆡ pH ߪ
3.0 ߢ޽ߞߚ‫ߚ߹ޕ‬, pH 3.0 ߦ߅ߌࠆหၮ⾰ߦኻߔࠆ Km ୯ߪ⚂ 1 PM ߣ᳞߼ࠄࠇߚ‫৻ޕ‬ᣇ, SAP ߦ
ኻߔࠆฦ⒳ࡊࡠ࠹ࠕ࡯࠯㒖ኂ೷ߩᓇ㗀ࠍᬌ⸛ߒߚ⚿ᨐ‫ޔ‬SAP ߪࡍࡊࠬ࠲࠴ࡦߦࠃߞߡߩߺ⪺ߒߊᵴᕈ߇㒖ኂߐࠇߚߎߣ߆ࠄ, ࠕࠬࡄ࡞࠹࠶ࠢࡊࡠ࠹ࠕ࡯࠯ࡈࠔࡒ࡝࡯ߦዻߔࠆߎߣ߇␜ߐࠇߚ‫ޕ‬߹ߚ, ࡍࡊࠬ࠲࠴ࡦߩ SAP ߦኻߔࠆ Ki ୯ߪ 25 fM ߣ᳞߼ࠄࠇߚ‫ޕ‬2) ⊒⴫ቇળ㧦╙ 16 ࿁⑺↰ᔕ↪↢๮⑼ቇ⎇ⓥળቇⴚ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 5 ᦬ 28 ᣣ‫✚⋵↰⑺ޔ‬ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧔⑺↰Ꮢ㧕Ṷ㗴ฬ㧦Sf-9 ᣸⯻⚦⢩-ࡃࠠࡘࡠ࠙ࠖ࡞ࠬ⊒⃻♽ߦ߅ߌࠆࠕࠬࡄ࡞࠹ࠖ࠶ࠢࡊࡠ࠹ࠕ࡯࠯ߩ
↢ᚑ᜼േߣ․ᕈ⸃ᨆ⊒⴫⠪㧦ዊ㊁ᵗノ, ᓟ⮮⁴, ⩵࿾⾫৻ (⑺↰ᄢቇ࡮Ꮏቇ⾗Ḯ)‫ޔ‬㖴Ỉᖗ (࿖㓙ㄘ⎇࠮)‫ޔ‬㜞ᯅ⍾❱ (⑺↰⋵✚㘩⎇࠮)‫⚵ޤ⸒✜ޣ‬឵߃࠲ࡦࡄࠢ⾰ߩ⇣⒳⊒⃻♽ߣߒߡߪ, ᄢ⣺⩶, ㉂Უ߿േ‛⚦⢩ߢߩ⊒⃻♽߇⍮ࠄࠇߡ޿ࠆ‫ޕ‬ᚒ‫⚵ߡ޿↪ࠍ♽⃻⊒ߩࠄࠇߎߪޘ‬឵߃ဳࡅ࠻࡟࠾ࡦߩ⊒⃻ࠍ⹜ߺߚ[1]‫⚿ߩߘޕ‬ᨐ, ᄢ⣺⩶ߢߪ⊒⃻࠲ࡦࡄࠢ⾰߇ኽ౉૕ࠍᒻᚑߔࠆߎߣ߆ࠄᵴᕈဳ㉂⚛ࠍᓧࠆߦߪนṁൻߣᏎ߈ᚯߒ߇ᔅⷐ
ߢ޽ߞߚ‫ߚ߹ޕ‬, ㉂Უ߿േ‛⚦⢩⊒⃻♽ߦ߅޿ߡߪߘߩ⊒⃻㊂ߦ໧㗴߇޽ߞߚ‫৻ޕ‬ᣇ, ᣸⯻⚦⢩-− 59 −

ࡃࠠࡘ࠙ࠖ࡞ࠬ⊒⃻♽ߦ߅ߌࠆ⚵឵߃ဳࡅ࠻࡟࠾ࡦ↢↥♽ߢߪ, ౝ࿷ᕈᵴᕈൻ㉂⚛ߩሽ࿷ߦࠃࠅ
ၭ㙃ᓟᦼߦᵴᕈဳ࡟࠾ࡦ߇ၭ࿾ਛߦ⫾Ⓧߔࠆߎߣࠍ⷗಴ߒߚ [2-5]‫ߒ߆ߒޕ‬, ၭ㙃ᦨᓟᦼߦߪ⋡⊛࠲ࡦࡄࠢ⾰ߩ෼㊂߇ૐਅߔࠆ௑ะ߇⷗ࠄࠇߚ‫⎇ᧄߢߎߘޕ‬ⓥߢߪ, ౝ࿷ᕈࡊࡠ࠹ࠕ࡯࠯ߩ৻ߟߢ
ࡍࡊࠬ࠲࠴ࡦߦࠃࠅᒝߊᵴᕈ㒖ኂࠍฃߌࠆࠕࠬࡄ࡞࠹ࠖ࠶ࠢࡊࡠ࠹ࠕ࡯࠯ (SAP) ߩ․ᕈ⸃ᨆࠍⴕߞߚ‫ޕ‬‫ޣ‬ታ㛎ᣇᴺ‫ޤ‬ᜰᢙᦼߩ Sf-9 ᣸⯻⚦⢩ Ộᶧߦࡅ࠻ࡊ࡟ࡊࡠ࡟࠾ࡦ cDNA ዉ౉ࡃࠠࡘࡠ࠙ࠖ࡞ࠬࠍ
ᗵᨴᄙ㊀ᐲ(MOI) 1 pfu/cells ߢᗵᨴߐߖ, 28͠ߢᠣᜈၭ㙃ࠍ㐿ᆎߒߚ‫ޕ‬ᗵᨴ 6 ᣣ⋡ߩၭ㙃ᶧࠍ࿁෼ߒ, Pepstatin-aminohexyl Sepharose, DEAE-Sepharose FF,߅ࠃ߮ Superdex-200 ࠞ࡜ࡓࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ ߦࠃࠅ SAP ߩ♖⵾ࠍⴕߞߚ‫ޕ‬SAP ߩᵴᕈ୯᷹ቯߦߪⰯశ⥄Ꮖᶖశၮ⾰ࠍ↪޿, 37͠ߢ෻ᔕᓟ,Ⱟశᒝᐲ㧔ബ⿠ᵄ㐳 328 nm, ᷹ቯᵄ㐳 393 nm㧕ࠍ᷹ቯߒߚ‫ޕ‬‫⚿ޣ‬ᨐߣ⠨ኤ‫ޤ‬ၭ㙃ᶧਛߩ SAP ᵴᕈߪ, ᗵᨴၭ㙃ߦ઻ߞߡࠁࠆ߿߆ߦ਄᣹ߒ, ⚦⢩߇ᱫṌߔࠆၭ
㙃 6 ᣣ⋡ߦᕆỗߦჇടߒߚ‫ߢߎߘޕ‬, ᗵᨴ 6 ᣣ⋡ߩၭ㙃ᶧ߆ࠄ SAP ߩ♖⵾ࠍ⹜ߺߚ‫ޕ‬200 ml ߩၭ㙃ᶧ߆ࠄ⚂ 29 Pg ߩ♖⵾ SAP ࠍᓧߚ‫ ⵾♖ޕ‬SAP ߩಽሶ㊂ߪࠥ࡞ỹㆊᴺߦࠃࠅ 37 kDa, ߹ߚ SDSPAGE ߢ 42 kDa ߣ᳞߼ࠄࠇߚ‫ߡߞࠃޕ‬, SAP ߪන㊂૕ߢሽ࿷ߔࠆߎߣ߇␜ໂߐࠇߚ‫ޕ‬N ᧃ┵ࠕࡒ
ࡁ㉄㈩೉ߩ BLAST ⸃ᨆߩ⚿ᨐ, SAP ߪࠞࠗࠦ߿࡚࡚ࠪ࠙ࠫ࠙ࡃࠛ↱᧪ߩࠞ࠹ࡊࠪࡦ D ߣ㜞޿⋧หᕈ߇⹺߼ࠄࠇߚ‫ޕ‬SAP ߩ⥋ㆡ pH ߪ 3.0 ߢ, ห pH ߦ߅ߌࠆ Km ୯ߪ⚂ 1 PM ߣ᳞߼ࠄࠇߚ‫ޕ‬ฦ⒳ࡊࡠ࠹ࠕ࡯࠯㒖ኂ೷ߩᓇ㗀ࠍᬌ⸛ߒߚ⚿ᨐ, SAP ߪࡍࡊࠬ࠲࠴ࡦߦࠃߞߡߩߺ㒖ኂ߇⏕⹺ߐࠇߚ‫ ࠄ߆ߣߎߩߎޕ‬SAP ߪࠕࠬࡄ࡞࠹ࠖ࠶ࠢࡊࡠ࠹ࠕ࡯࠯ࡈࠔࡒ࡝࡯ߦዻߔࠆߎߣ߇␜ߐࠇߚ‫߹ޕ‬ߚ, SAP ᵴᕈߦ߅ࠃ߷ߔࡍࡊࠬ࠲࠴ࡦߩỚᐲଐሽᕈߦߟ޿ߡಽᨆࠍⴕߞߚ⚿ᨐ, SAP ߩ 50%㒖ኂ୯ߪ 38 pM ߣ᳞߼ࠄࠇ, ࡍࡊࠬ࠲࠴ࡦߩ SAP ߦኻߔࠆ Ki ୯ߪ 25 fM ߣ᳞߼ࠄࠇߚ‫ޕ‬‫ޣ‬ෳ⠨ᢥ₂‫ޤ‬1) Takahashi S. et al. Bioscience Biotechnology and Biochemistry, 70, 2918-2918 (2006), 2)
Takahashi S. et al. ibid, 72, 3232-3236 (2008), 3) Gotoh T., et al. ibid, 74, 370-374 (2010), 4) Gotoh T. et al.
Biochemical Engineering Journal, 43, 216-220 (2009), 5) Gotoh T. et al. Journal of Chemical Engineeringof Japan, in press ⊒⴫ቇળ㧦╙  ࿁⑺↰ᔕ↪↢๮⑼ቇ⎇ⓥળቇⴚ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫✚⋵↰⑺ޔ‬ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧔⑺↰Ꮢ㧕Ṷ㗴ฬ㧦ේᩭᓸ↢‛↱᧪ &ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯㧔2CGPKFCUG㧕ㆮવሶߩ
ࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯߆ࠄߩන㔌⊒⴫⠪㧦㖴Ỉ ᖗ㧔⁛ᴺ࿖㓙ㄘᨋ᳓↥ᬺ⎇ⓥ࠮㧕‫ޔ‬㜞ᯅ⍾❱㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮㧕‫৻ޔߢ߹ࠇߎޤ⊛⋡ޣ‬ㇱߩ⚦⩶ߩ⚦⢩⤑ߦ D ဳࠕࡒࡁ㉄ߩሽ࿷ߔࠆߎߣ߇⍮ࠄࠇߡ޿ߚ߇‫ޔ‬ㄭᐕ‫ޔ‬ູ੃㘃ߩ↢૕ౝߦ߽ㆆ㔌ߩ D ဳࠕࡒࡁ㉄߿ D ဳࠕࡒࡁ㉄ࠍ฽᦭ߔࠆ࠲ࡦࡄࠢ⾰ߩሽ࿷ߔࠆߎߣ߇
⷗಴ߐࠇߡ޿ࠆ‫ޕ‬㜞ᯅࠄߪ D-ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯↢↥⩶㧔Paenibacillus sp. B38 ᩣ㧕ࠍಽ㔌ߔࠆߣߣ߽ߦ‫ࠆߔ↥↢ޔ‬㉂⚛ࠍ paenidase ߣ๮ฬߒ‫ߩߘޔ‬ᕈ⾰ࠍ᣿ࠄ߆ߦߒߚ 1)‫੹ޕ‬࿁ᚒ‫ޘ‬ߪ‫ޔ‬PCR ᴺߦࠃࠅขᓧߒߚ paenidase ㆮવሶߩႮၮ㈩೉ࠍౣ⏕⹺ߔࠆߚ߼ߦ‫࡯࡝࡜ࡉࠗ࡜ࡓࡁࠥޔ‬ࠃࠅㆮવሶࠍන㔌ߒ‫ߩߘޔ‬㈩೉ࠍ⺞ߴߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ޤ‬Paenibacillus sp. B38 ᩣࠃࠅࠥࡁࡓ DNA ࠍන㔌ߒ‫⿥ޔ‬㖸ᵄ⎕⎈ⵝ⟎ࠍ↪޿ߡ⚂ 4 kb ߩࠨࠗ࠭ߦߥࠆࠃ߁ߦᢿ

ൻߒߚߩߜ‫ ࠼ࡒࠬ࡜ࡊޔ‬DNA pUC118 ߦ⚿วߒߡࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯ࠍ૞

ᚑߒߚ‫ޔߦ߉ߟޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ࠍᄢ⣺⩶ DH10B ᩣߦዉ౉ߒ‫࡯࠾ࡠࠦޔ‬PCR ᴺߦࠃࠅ⋡⊛− 60 −

㈩೉ࠍ฽߻ࠢࡠ࡯ࡦࠍࠬࠢ࡝࡯࠾ࡦࠣߒߚ‫ޕ‬‫⚿ޣ‬ᨐ෸߮⠨ኤ‫⹏ߩ࡯࡝࡜ࡉࠗ࡜ޤ‬ଔߩߚ߼ߦ‫ޔ‬ᄢ⣺⩶ࠦࡠ࠾࡯߆ࠄ 16 ୘ߩࠢࡠ࡯ࡦࠍㆬᛯߒ‫ޔ‬ߎࠇࠄߦ฽߹ࠇࠆࠗࡦࠨ࡯࠻ߩࠨࠗ࠭ࠍࠕࠟࡠ࡯ࠬࠥ࡞㔚᳇ᵒേߦࠃࠅ⏕⹺ߒߚߣߎࠈ‫ޔ‬3-5 kbߩࠗࡦࠨ࡯࠻߇ 70% એ਄ࠍභ߼ࠆߎߣ߇ࠊ߆ߞߚ‫ ࠄ߆࡯࡝࡜ࡉࠗ࡜ࡓࡁࠥޕ‬Paenidase ㆮવሶࠍ฽ ߻ ࠢ ࡠ ࡯ ࡦ ࠍ ࠬ ࠢ ࡝ ࡯ ࠾ ࡦ ࠣ ߒ ߚ ߣ ߎ ࠈ ‫ ޔ‬3,804bp ߩ ࠗ ࡦ ࠨ ࡯ ࠻ ࠍ ᜬ ߟ ࡊ ࡜ ࠬ ࡒ ࠼㧔pUC118/K19㧕ࠍන㔌ߒߚ‫ޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ߩႮၮ㈩೉ࠍಽᨆߒ‫ޔ‬PCR ᴺߦࠃࠅᓧࠄࠇߚႮ
ၮ㈩೉ߣᲧセߒߚߣߎࠈ‫ޔ‬⠡⸶㗔ၞߢߪႮၮߩ㆑޿ߪߥ߆ߞߚ߇‫ޔ‬5’஥ߩ㕖⠡⸶㗔ၞߢ㈩೉߇⚵ߺ឵ࠊߞߡ޿ߚ‫ޔߢߎߘޕ‬ౣᐲ 5’஥ߩ㕖⠡⸶㗔ၞࠍ฽߻ࠢࡠ࡯ࡦࠍࠬࠢ࡝࡯࠾ࡦࠣߒߚߣߎࠈ‫ޔ‬4,071bp ߩࠗࡦࠨ࡯࠻ࠍᜬߟࡊ࡜ࠬࡒ࠼㧔pUC118/64㧕ࠍන㔌ߒߚ‫ޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ߩႮၮ㈩೉ࠍಽᨆߒ‫ޔ‬pUC118/K19 ߩႮၮ㈩೉ߣ⍦⋫߇ߥ޿ߎߣࠍ⏕⹺ߒߚ‫੹ޕ‬࿁⸃ᨆߒߚ㗔ၞߦߪ
paenidase ㆮવሶࠍ฽߼ߡ᭴ㅧㆮવሶ߇ 4 ⒳㘃ሽ࿷ߔࠆߣផቯߐࠇߚ‫ޕ‬Paenidase એᄖߩㆮવሶߪ‫ޔ‬ߘࠇߙࠇ Clostridium cellulolyticum ↱᧪ Cellulosome anchoring protein (30%)‫ޔ‬Brevibacillus brevis ↱᧪ ATPase ᭽࠲ࡦࡄࠢ⾰ (73%)‫ޔ‬ห↱᧪ 4-aminobutyrate aminotransferase ᭽࠲ࡦࡄࠢ⾰ (48%)ߣ⋧หᕈ߇޽ߞߚ‫ޕ‬‫ޣ‬ෳ⠨ᢥ₂‫ޤ‬
1. S. Takahashi et al., 㧶. Biochem. 139, 197-202 (2006).
4) ⊒⴫ቇળ䋺╙ 㪋㪐 ࿁ᣣᧄ↢૕කᎿቇળᄢળ㩷⊒⴫ᣣ䈫႐ᚲ䋺2010 ᐕ 6 ᦬ 25 ᣣ䇮ᄢ㒋࿖㓙੤ᵹ䉶䊮䉺䊷䋨ᄢ㒋Ꮢ䋩Ṷ㗴ฬ䋺Study on evaluation of food by ERP㩷 P300 in the case while changing the number of displayed
pictures㩷 corresponding to each opinion⊒⴫⠪䋺Tomohiro Honma*䇮Motoshi Tanaka*䇮Hiroshi Inoue*䇮Yoshitsugu Niiyama*䇮
Takahashi Toru**䇮Masanori Kumagai**䇮Yoshinobu Akiyama***䋨*Akita University䇮**Akita Research Institute for Food and Brewing䇮***Akita Prefectural University䋩
1. 䈲䈛䉄䈮㩷 ↹௝䈪㘩ຠ䉕ឭ␜䈚䈩䇮ᅢ䉂䉕ਥⷰ⹏ଔ䈜䉎䈫䈐䈱੐⽎㑐ㅪ㔚૏䋨ERP䋩䇮․䈮 P300 ᚑಽ䉕ਥⷰ⹏ଔ䈱ቯ㊂⊛ᜰᮡ䈫䈜䉎䈖䈫䉕ᬌ⸛䈚䈩䈐䈢䇯䈖䉏䉁䈪䈱⚿ᨐ䈪䈲䇮⹏ଔ⺆䈮ኻᔕ䈜䉎↹௝䈱ᨎᢙ䈱⚵䉂ว䉒䈞䈮䉋䈦䈩⹏ଔ⺆䈱㗅䋨䈫䈩䉅ᅢ䈐䇮ᰴ䈮ᅢ䈐䇮᥉ㅢ䈮ᅢ䈐䋩䈫 㪧㪊㪇㪇㩷 䈱ᄢ䈐䈘䈱㗅䈏৻⥌䈚䈭䈇႐ว䈏䈅䈦䈢䇯䉁䈢䇮⺖㗴䈏㔍䈚䈇䈫 㪧㪊㪇㪇㩷 ᝄ᏷䈏ዊ䈘䈒䈭䉎䈫䈱ႎ๔䈏䈅䉎䇯䈠䈖䈪ᧄ⎇ⓥ䈪䈲䇮㘩ຠ⹏ଔᤨ䈮ਈ䈋䉎⺖㗴ᬌ⸛䈱䈢䉄䈮䇮ฦ⹏ଔ⺆䈮ኻᔕ䈜䉎↹௝䈱ᨎᢙ䉕ᄌ䈋䈢⺖㗴䈱㔍䈚䈘䈮䉋䉎ലᨐ䈮䈧䈇䈩ᬌ⸛䈚䈢䇯㩷㪉㪅㩷 ታ㛎ᣇᴺ㩷 ⹏ଔ↪↹௝䈮䈲䇮䉬䊷䉨䈱౮⌀↹௝ 㪈㪇㩷 ᨎ䈫䈚䇮䈠䈱ਛ䈎䉌ฦታ㛎೨䈮䇮䈫䈩䉅ᅢ䈐䈭䉅䈱䇮ᰴ䈮ᅢ䈐䈭䉅䈱䇮᥉ㅢ䈮ᅢ䈐䈭䉅䈱䉕䇮ⵍ㛎⠪䈮䈠䉏䈡䉏ㆬ䈳䈞䈢䇯㪜㫏㫇㪅㩷 㪘㩷 䈲ᢥ₂㪲㪈㪴䈪↪䈇䈢⚵䉂ว䉒䈞䈫ห䈛䈫䈚䈢䇯㪜㫏㫇㪅㩷㪙㩷 䈲䇮↹௝✚ᢙ䉕 㪜㫏㫇㪅㩷㪘㩷 䈫ห䈛 㪌㩷 ᨎ䈫䈚䇮䇸ᰴ䈮ᅢ䈐䇹䈱⹏ଔ䈏㔍䈚䈒䈭䉎䉋䈉䈮 㪉㩷ᨎ䈫䈚䈢䇯㪜㫏㫇㪅㩷 㪚㩷 䈲ᢥ₂㪲㪉㪴㩷 䈪↪䈇䈢⚵䉂ว䉒䈞䈪䈅䉍䇮㪊㩷 ⺖㗴ਛ䉅䈦䈫䉅㔍䈚䈇䈫⠨䈋䉌䉏䉎䇯ⵍ㛎⠪䈮䈲䇮㪊 Ბ㓏ᅢ䉂ዤᐲ䈪⹏ଔ䈜䉎䊗䉺䊮᛼䈚⺖㗴䉕ਈ䈋䈢䇯ⵍ㛎⠪ᢙ䈲 㪊㪇 ฬ䋨㪈㪏䌾㪉㪌㩷 ᱦ䋩䈪䇮หᗧ䉕ᓧ䈢䈉䈋䈪ታ㛎䉕ⴕ䈦䈢䇯㩷㪊㪅㩷 ⚿ᨐ䈫ᬌ⸛㩷 㪜㫏㫇㪅㩷 㪘㩷 䈪䈲䇮㪧㪊㪇㪇㩷 䈏↹௝ឭ␜ᓟ 㪉㪌㪇䌾㪌㪇㪇㫄㫊㩷 䈮⃻䉏䈩䈍䉍䇮䈖䈱▸࿐䈱ട▚ᐔဋᵄ
ᒻ䈱㕙Ⓧ䋨Ⓧಽ୯䋩䉕 㪧㪊㪇㪇㩷 㕙Ⓧ䈫ቯ⟵䈚䈢䇯㔚ᭂ૏⟎ 㪧㫑 䈱 㪜㫏㫇㪅㩷㪘䌾㪚㩷 䈱 㪧㪊㪇㪇 㕙Ⓧ䋨ⵍ㛎⠪Ფ䈮ᦨᄢ୯䈪ᱜⷙൻ䋩䈱⚿ᨐ䋨ᐔဋ୯䈫ᮡḰ஍Ꮕ䋩䇮㪜㫏㫇㪅㩷㪘 䈪䈲䇮䇸䈫䈩䉅ᅢ䈐䇹䈫⹏ଔ䈚䈢䈫䈐䈱㕙Ⓧ䈏ᦨ䉅ᄢ䈐䈒䇮− 61 −

ᢥ₂㪲㪈㪴䈱௑ะ䈫৻⥌䈚䈢䇯㪜㫏㫇㪅㩷 㪙 䈪䈲䇮ฦ⹏ଔ⺆㑆䈱㕙Ⓧ䈱Ꮕ䈏ዊ䈘䈒䈭䈦䈩䈍䉍䇮㪜㫏㫇㪅㩷 㪘㩷 䈫Ყ䈼䈩㔍䈚䈒ᗵ䈛䈢䉅䈱䈫⠨䈋䉌䉏䉎䇯㪜㫏㫇㪅㩷 㪚㩷 䈪䈲䇮䇸ᰴ䈮ᅢ䈐䇹䈱䈫䈐䈮ᦨ䉅ዊ䈘䈒䇮ᢥ₂㪲㪉㪴䈫ห᭽䈭⚿ᨐ䈪䈅䉎䇯䈖䉏䉌䈱⚿ᨐ䈎䉌䇮ᅢ䉂⹏ଔᤨ䈲䇸䈫䈩䉅ᅢ䈐䇹㪈 ᨎ䇮䇸ᰴ䈮ᅢ䈐䇹㪈 ᨎ䉕ⵍ㛎⠪䈮⹏ଔ䈘䈞䉎⺖㗴䈏䉋䈇䈫⠨䈋䉌䉏䉎䇯㩷 ⊒⴫ቇળ㧦╙  ࿁ࠠ࠴ࡦ࡮ࠠ࠻ࠨࡦ࡮ࠪࡦࡐࠫ࠙ࡓ
⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫᧲ޔ‬੩ᄢቇ㧔᧲੩ㇺᢥ੩඙㧕Ṷ㗴ฬ㧦ࡃࠠࡘࡠ࠙ࠗ࡞ࠬᗵᨴ᣸⯻⚦⢩ 5H ↱᧪ࠠ࠴࠽࡯࠯ߩ෻ᔕ․ᕈ
⊒⴫⠪㧦᳓ේ㤗⟤ሶ㧘ᓟ⮮ ⁴㧖㧘㜞ᯅ⍾❱㧖㧖㧘ᷓḴ ᘮ㧔ㄭ⇰ᄢ㒮࡮ㄘ࡮ࡃࠗࠝ
⑺↰ᄢ㒮࡮Ꮏቇ⾗Ḯ㧘㧖㧖⑺↰⋵࡮✚㘩⎇࠮㧕㧖

Expression and characterization of chitinase produced by Baculovirus(AcMNPV)-infected Sf-9 insect
cell expression system.
Mamiko Mizuhara, Takeshi Gotoh*, Saori Takahashi**, Tamo Fukamizo
Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan
E-mail:fukamizo@nara.kindai.ac.jp
Baculovirus(AcMNPV)-infected Sf-9 cells produced chitinase in the late stage of cultivation. The
chitinase was purified nearly homogeneity by single step pepstatin-aminohexyl-Sepharose column
chromatography. The purified chitinase showed single protein band on SDS-PAGE with the weight of
65,000. The N-terminal sequence of the enzyme was identical to chitinase from AcMNPV gene.

The

enzyme was found to hydrolyze the second glycosidic linkage of (GlcNAc)6 producing (GlcNAc)2 and(GlcNAc)4.

The transglycosylation products were also detected in the reaction mixture of theoligosaccharide substrates.

The mode of action of the AcMNPV chitinase was similar to that of bacterial

and fungal family GH-18 chitinases.
ߎࠇ߹ߢߦ‫࡮ࠬ࡞ࠖ࠙ࠬࡠࡘࠠࡃޔ‬᣸⯻⚦⢩⊒⃻♽ߦ߅޿ߡ⚵឵߃ဳࡅ࠻ࡊ࡟ࡊࡠ࡟࠾ࡦ߇ᄢ
㊂ߦ⊒⃻ߐࠇࠆߎߣ, ߹ߚၭ㙃ᓟᦼߦ߅޿ߡ AcMNPV ↱᧪ߩࠠ࠴࠽࡯࠯߇ઃ㓐ߒߡ⊒⃻ߐࠇࠆߎߣࠍߺ޿ߛߒߡ޿ࠆ㧚ᧄ⎇ⓥߢߪ㧘ߎߎߢᓧࠄࠇߚ AcMNPV ↱᧪ࠠ࠴࠽࡯࠯㧔AcMNPV-Chi㧕ߩട᳓ಽ⸃᭽ᑼࠍ⺞ߴࠆߎߣࠍ⋡⊛ߣߒߚ㧚AcMNPV-Chi ߦࠃࠆࡋࠠࠨࠝ࡝ࠧ♧ട᳓ಽ⸃ߩ⚻ᤨᄌൻࠍ HPLC ߢ⺞ߴߚ⚿ᨐ‫(ޔ‬GlcNAc)6 ߩ㕖ㆶరᧃ┵߆ࠄੑ⇟⋡ߩࠣ࡝ࠦࠪ࠼⚿ว߇ఝవ⊛ߦട᳓ಽ⸃ߐࠇ‫(ޔ‬GlcNAc)2 ߣ(GlcNAc)4 ࠍ↢ᚑߔࠆߎߣ߇ࠊ߆ߞߚ㧚߹ߚ෻ᔕ↢ᚑ‛ߩ MALDI-TOF-MASSಽᨆࠍⴕߞߚߣߎࠈ㧘᦭ᗧߥ㊂ߩ♧ォ⒖෻ᔕ↢ᚑ‛ࠍᬌ಴ߒߚ㧚ߎࠇࠄߩ⚿ᨐߪ‫⺞ߦߢ߹ࠇߎޔ‬ߴࠄࠇߡ޿ࠆᓸ↢‛↱᧪ Family GH-18 ࠠ࠴࠽࡯࠯ߩട᳓ಽ⸃᭽ᑼߣ㕖Ᏹߦ㘃ૃߒߡ߅ࠅ‫ࡁࡒࠕޔ‬㉄㈩೉ߩ⋧หᕈߣ⍦⋫ߩߥ޿⚿ᨐ߇ᓧࠄࠇߚ‫ޕ‬6) ⊒⴫ቇળ䋺╙䋱䋳࿁ᣣᧄశ⣖ᯏ⢻䉟䊜䊷䉳䊮䉫⎇ⓥળ㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ䋷᦬ 㪉㪋 ᣣ䇮ᤊ㒺ળ㙚䋨᧲੩ㇺ䋩㩷Ṷ㗴ฬ䋺㘩ຠ䈱ᄖ༵ⷰᅢ⹏ଔᤨ䈮䈍䈔䉎೨㗡೨㊁ዪᚲ⣖ⴊᵹേᘒ䈱⸃ᨆ㩷⊒⴫⠪䋺䂾ᾢ⼱᣽ೣ䋪䇮㜟ᯅᔀ䋪䇮⑺ጊ⟤ዷ䋪䋪㩷 㩷
䋨䋪⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䇮䋪䋪⑺↰⋵┙ᄢቇ䋩㩷− 62 −

㘩ຠ䈱䈍䈇䈚䈘䈭䈬䈫䈇䈦䈢༵ᅢᕈ䉇ᗵᕈ䈮ଥ䉒䉎䊍䊃䈱ᔕ╵䈲䇮䉝䊮䉬䊷䊃⺞ᩏᴺ䉇ቭ⢻ᬌᩏᴺ䈭䈬䈮䉋䈦䈩᛽಴䈜䉎䈱䈏৻⥸⊛䈪䈅䉎䇯䈚䈎䈚䈭䈏䉌䇮ቴⷰᕈ䈭䈬䈮ᰳ䈔䉎႐ว䈏䈅䉍䇮ઍᦧᴺ䈱㐿⊒䈏᳞䉄䉌䉏䈩䈇䉎䇯䈠䈖䈪ᚒ䇱䈲䇮㘩ຠ⹏ଔᤨ䈮䈍䈔䉎ⵍ㛎⠪䈱༵ᅢ䉕䇮⣖ⴊᵹᄌൻ䉕ᜰᮡ䈫䈚䈩䇮ᄢ⣖⴫ጀ䈎䉌⋥ធ䇮⺒䉂ข䉏䈭䈇䈎䈬䈉䈎ᬌ⸛䈚䈩䈇䉎䇯ⵍ㛎⠪䈮䇮㘩ຠ↹௝䉕ⷞⷡೝỗ䈫䈚䈩ਈ䈋䇮䈠䈱↹௝䈮䈧䈇䈩༵ᅢᕈ䉕⹏ଔ䈘䈞䈩䈇䉎ᤨ䈱೨㗡೨㊁䈮䈍䈔䉎ዪᚲ⣖ⴊᵹᄌൻ䉕䇮ㄭ⿒ᄖಽశᴺᑼ䈮䉋䉎⣖ⴊᵹ᷹ቯⵝ⟎䋨㪥㪠㪩㪪䋩䈪᷹ቯ䈚䇮⸃ᨆ䈚䈢䇯䈠䈱⚿ᨐ䇮䈍䈇䈚䈠䈉䈫ᕁ䈦䈩↹௝䉕⷗䈩䈇䉎䈫䈐䈫䇮䈠䈉䈪䈭䈇䈫䈐䈪䈲䇮⣖ⴊᵹ䈱ᄌൻ㊂䈮㆑䈇䈏⹺䉄䉌䉏䇮ⵍ㛎⠪䈱༵ᅢ್ᢿ䉕 㪥㪠㪩㪪 䈪੍᷹䈪䈐䉎น⢻ᕈ䈏␜ໂ䈘䉏䈢䇯㩷㪎㪀㩷 ⊒⴫ቇળ䋺ᐔᚑ 㪉㪉 ᐕᐲ㔚᳇㑐ଥቇળ᧲ർᡰㇱㅪวᄢળ㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ 㪏 ᦬ 㪉㪍 ᣣ䇮౎ᚭᄢቇ䋨㕍᫪⋵౎ᚭᏒ䋩㩷Ṷ㗴ฬ䋺䉬䊷䉨䉕଀䈫䈚䈢㘩ຠ䈱ᅢ䉂⹏ଔᤨ䈱 㪜㪩㪧 ⸘᷹㩷⊒⴫⠪䋺䂾↰ਛరᔒ䇮ᧄ㑆ᥓᄢ䇮੗਄㩷 ᶈ䇮ᣂጊ༑༹䇮㜞ᯅ㩷 ᔀ㺒䇮㩷 ᾢ⼱᣽ೣ㺒䇮㩷 ⑺ጊ⟤ዷ㺒㺒㩷䋨⑺↰ᄢቇ䇮㺒⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䇮㺒㺒⑺↰⋵┙ᄢቇ䋩㩷㪈㪅㩷 䉁䈋䈏䈐㩷 ੐⽎㑐ㅪ㔚૏䋨㪜㪩㪧䋩䉕ᜰᮡ䈫䈚䈢㘩ຠ⹏ଔᴺ䈱㐿⊒䉕⋡⊛䈫䈚䇮ኼม䉇㈬↉䈭䈬䈱౮⌀↹௝䉕ឭ␜䈚䈩䇮ᅢ䉂䈪ਥⷰ⹏ଔ䈘䈞䈢䈫䈐䈱 㪜㪩㪧䇮․䈮 㪧㪊㪇㪇㩷 ᚑಽ䈮䈧䈇䈩ᬌ⸛䈚䈩䈐䈢䇯㪧㪊㪇㪇㩷 ᚑಽ䈮ⶄᢙ䈱䊏䊷䉪䈏⃻䉏䈢䈖䈫䈎䉌䇮⹏ଔᜰᮡ䈫䈚䈩 㪧㪊㪇㪇 䈱㕙Ⓧ䈱೑↪䉕ឭ᩺䈚䈢䇯䉁䈢䇮䇸䈫䈩䉅ᅢ䈐䇹䈫⹏ଔ䈚䈢䈫䈐䈮 㪧㪊㪇㪇㩷 㕙Ⓧ䈲ᦨ䉅ᄢ䈐䈒䈭䈦䈢䇯ᧄⓂ䈪䈲䇮ઁ䈱㘩ຠ䈱଀䈫䈚䈩䉬䊷䉨䉕ข䉍਄䈕䇮䈠䈱↹௝䉕ឭ␜䈚䈩䇮ᅢ䉂䋨ᅢ䈐䋩䈱⒟ᐲ䉕 㪊㩷 Ბ㓏䈱⹏ଔ⺆䈪⹏ଔ䈘䈞䈢䈫䈐䈱 㪜㪩㪧㩷 䉕䇮ᅚᕈ䈫↵ᕈ䈮䈧䈇䈩᷹ቯ䈚䈢䈱䈪ႎ๔䈜䉎䇯㩷㪉㪅㩷 ታ㛎ᣇᴺ㩷 䉬䊷䉨䉕᠟ᓇ䈚䈢↹௝䈱ਛ䈎䉌䇮ⵍ㛎⠪䈮䈫䈩䉅ᅢ䈐䈭䉅䈱 㪈㩷 ᨎ䇮ᰴ䈮ᅢ䈐䈭䉅䈱 㪈㩷 ᨎ䇮᥉ㅢ䈮ᅢ䈐䈭䉅䈱 㪊㩷 ᨎ䈱⸘ 㪌㩷 ᨎ䉕ㆬ䈳䈞䇮ታ㛎䈮↪䈇䈢䇯ⵍ㛎⠪䈮䈲䇮↹௝䈏ឭ␜䈘䉏䈢䉌䈜䈓䈮䇮䇸䈫䈩䉅ᅢ䈐䋨⹏ଔ୯ 㪊䋩䇹䇮䇸ᰴ䈮ᅢ䈐䋨㪉䋩䇹䇮䇸᥉ㅢ䈮ᅢ䈐䋨㪈䋩䇹䈱 㪊㩷 Ბ㓏ዤᐲ䈪⹏ଔ䈚䇮ฦ⹏ଔ⺆䈮ኻᔕ䈜䉎䊗䉺䊮䉕᛼䈜䉋䈉䈮ᜰ␜䈚䈢䇯㩷㪊㪅㩷 ⚿ᨐ䈫ᬌ⸛㩷 㪧㪊㪇㪇㩷 䈱ᝄ᏷䈲ᅚᕈ䈱ᣇ䈏ో૕⊛䈮ᄢ䈐䈇௑ะ䈏䉂䉌䉏䈢䇯ẜᤨ⚂ 㪊㪇㪇㩷㫄㫊㩷 䈱䊏䊷䉪䈲↹௝ឭ␜䈮㑐ㅪ䈜䉎 㪧㪊㪸㩷 䈫⠨䈋䉌䉏䉎䈏䇮⹏ଔ䈮䉋䈦䈩ᝄ᏷䈮㆑䈇䈏䉂䉌䉏䉎႐ว䈏䈅䈦䈢䇯䈠䈖䈪䇮ฦⵍ㛎⠪䈮䈧䈇䈩䇮ട▚ᐔဋᵄᒻ䈱ẜᤨ 㪉㪌㪇䌾㪌㪇㪇㩷 㫄㫊 䈱㕙Ⓧ䋨㪧㪊㪇㪇㩷 㕙Ⓧ䋩䉕᛽಴䈚䈢䇯ⵍ㛎⠪䈮䉋䈦䈩
ᦨᄢ㕙Ⓧ䈲⇣䈭䉎䈱䈪䇮ⵍ㛎⠪Ფ䈮ᦨᄢ୯䈪ᱜⷙൻ䉕ⴕ䈦䈢䇯↵ᕈ䈱⚿ᨐ䈪䈲䇮⹏ଔ䈮䉋䉎㆑䈇䈏ዊ䈘䈎䈦䈢䇯䈖䈱ὐ䈮䈧䈇䈩䈱ᬌ⸛䈲੹ᓟ䈱⺖㗴䈪䈅䉎䇯↵ᅚ䈬䈤䉌䈱႐ว䈍䈇䈩䉅ᅢ䉂䈱㆑䈇䈮䉋䈦䈩㪧㪊㪇㪇㩷 㕙Ⓧ䈲⇣䈭䉍䇮䈫䈩䉅ᅢ䈐䈫⹏ଔ䈚䈢䈫䈐䈮ᦨ䉅ᄢ䈐䈇ⵍ㛎⠪䈏ᄙ䈒䇮䈖䉏䉁䈪䈱ታ㛎⚿ᨐ䈫ห᭽䈱⚿ᨐ䈫䈭䈦䈢䇯㩷㩷㩷㪏㪀㩷 ⊒⴫ቇળ䋺The 23rd Annual and International Meeting of the Japanese Association for Animal
Cell Technology (JAACT2010)⊒⴫ᣣ䈫႐ᚲ䋺2010 ᐕ 9 ᦬ 2 ᣣ䇮ർᶏ㆏ᄢቇ䋨ᧅᏻᏒ䋩Ṷ㗴ฬ䋺Lupeol reduces lipid synthesis in adipocyte and hepatocyte⊒⴫⠪䋺Keishi Hata1 , Junichiro Takahashi2− 63 −

1

Akita Research Institute for Food & Brewing (ARIF), Akita, 010-1623, Japan,

2

Skylight Biotech. Inc., Akita, 010-0911, Japan
Lupeol, a lupane triterpenes is widely distributed in plant kingdom. In our previous study, lupeol was

found to suppress the accumulation of visceral adipose tissue by normalizing the serum lipoprotein profiles
in high fat-diet fed mice. In the present study, we studied the effects of lupeol on lipid synthesis in
adipocyte and hepatocyte.
Lupeol inhibited the TG accumulation in mature adipocytes. 3T3-L1 cell differentiation is regulated bysome transcription factors such as C/EBP-, and adipogenic enzymes were up-regulated as downstream
effectors of these transcription factors in cell differentiation. Lupeol significantly attenuated adipogenictranscription factors and enzymes such as C/EBP- and FAS, at mRNA level in mature 3T3-L1 cells.
Lupeol inhibited both TG and cholesterol secretion from HepG2 cells without affecting cell viability. We
examined whether lupeol affects the expression of various genes required for TG and cholesterol synthesis
in HepG2 cells. As the results, lupeol markedly reduced the lipogenic and cholesterologenic transcription
factors and enzymes in HepG2. Furthermore, lupeol attenuated the gene expression of apoB-100; however,the suppression of apoA-1 in HepG2 cells by lupeol was weak.
㩷㩷9) ቇળ⊒⴫㧦ᣣᧄ㘩ຠ⑼ቇᎿቇળ╙ 57 ࿁ᄢળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 2 ᣣ‫᧲ޔ‬੩ㄘᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦㤕⩶ᩣ࡜ࠗࡉ࡜࡝࡯ᵴ↪ߦࠃࠆ☨๧ཬߩࠗ࠰ࡑ࡞࠻࡯ࠬჇടലᨐ
⊒⴫⠪㧦ᷰㄝ㓉ᐘ‫ޔ‬૒⮮ᕺ㉿‫ޔ‬૒‫ᧁޘ‬ᐽሶ‫ޔ‬૒⮮ീ㧝‫ޔ‬ⅶ↢៨㧝‫੹ޔ‬㊁ብ㧝㧔⑺↰⋵✚㘩⎇࠮ࡦ࠲࡯‫ޔ‬㧝㧔ᩣ㧕⑺↰੹㊁໡ᐫ㧕‫☨ߪࠬ࡯࠻࡞ࡑ࠰ࠗޤ⊛⋡ޣ‬๧ཬߩ♧㘃ߢࠣ࡞ࠦ࡯ࠬߦᰴ޿ߢ㧞⇟⋡ߦᄙ޿♧ߢ޽ࠅ‫ޔ‬Ⓩ߿߆ߥ
↞ߺࠍ᦭ߔࠆߎߣ߆ࠄ‫↞ߦ․ޔ‬ญ๧ཬߢߪ๧ߦ㊀ⷐߥᓇ㗀ࠍਈ߃ࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬ ⒳㤕ࡔ࡯ࠞ࡯ߣ౒หߢ᭴▽ਛߩ㤕⩶࡜ࠗࡉ࡜࡝࡯ࠍᵴ↪‫♧ޔ‬ォ⒖ᵴᕈ߇ᦼᓙߢ߈ࠆ -ࠣ࡞ࠦࠪ࠳࡯࠯ᵴᕈߩ㜞޿㤕⩶ᩣࠍㆬᛯ‫☨ޔ‬๧ཬߩࠗ࠰ࡑ࡞࠻࡯ࠬ฽㊂Ⴧടࠍ⹜ߺߚ‫ޕ‬‫ޣ‬ᣇᴺ‫☨ߚ޿↪ࠍ࡟࡯ࡖࠪ ޤ‬㤕ߩ⵾ㅧ⹜㛎ࠍታᣉߒ -ࠣ࡞ࠦࠪ࠳࡯࠯ᵴᕈߩ㜞޿⩶ᩣߩㆬᛯࠍ
ⴕߞߚ‫ޕ‬ㆬᛯߒߚᵴᕈߩ㜞޿㤕⩶ᩣࠍ↪޿ේᢱ☨ 1kg ⷙᮨߩ⵾㤕⹜㛎‫઀߮ࠃ߅ޔ‬ㄟߺ✚㊀㊂ 3kgⷙᮨߩ๧ཬ⵾ㅧ⹜㛎ࠍታᣉߒߚ‫♧ޕ‬ಽᨆࠪࠬ࠹ࡓ㧔࠳ࠗࠝࡀ࠶ࠢࠬ␠㧕ߦࠃࠆ♧⚵ᚑߩ⚻ᤨ⊛ಽᨆߣᾫᚑ⚳ੌᤨߩቭ⢻ᬌᩏࠍታᣉߒߚ‫ޕ‬‫⚿ޣ‬ᨐ‫☨ ޤ‬㤕 139 ὐߩ -ࠣ࡞ࠦࠪ࠳࡯࠯ᵴᕈߪᐔဋ୯ 0.153u/g ߢ޽ࠅᦨᄢ୯ࠍ␜ߒߚ AOK139
ߩᵴᕈߪ 0.564u/g ߢ޽ߞߚ‫ޕ‬-ࠣ࡞ࠦࠪ࠳࡯࠯ᵴᕈߩ㜞ߐߣ㉂⚛ࡃ࡜ࡦࠬ߆ࠄㆬᛯߒߚ㧣ᩣ
㧔AOK51‫ޔ‬AOK52‫ޔ‬AOK139‫ޔ‬AOK209‫ޔ‬AOK214‫ޔ‬AOK232‫ޔ‬AOK233 ߦߟ޿ߡߩ๧ཬ⵾ㅧ⹜㛎ࠍታᣉߒߚ⚿ᨐ‫ ߽ᦨޔ‬-ࠣ࡞ࠦࠪ࠳࡯࠯ᵴᕈߩ㜞޿ AOK139 ࠍ↪޿ߚ๧ཬߩࠗ࠰ࡑ࡞࠻࡯ࠬ฽㊂
ߪ 2.61%ߢ޽ࠅ‫ޔ‬ኻᾖ㧔Ꮢ⽼⒳㤕㧕ߩ 1.76%ࠃࠅ߽Ⴧടߒߡ޿ࠆߎߣ߇⹺߼ࠄࠇߚ‫ޕ‬AOK139 ࠍ↪
޿ߚ๧ཬߪቭ⢻ᬌᩏߩ⚿ᨐ‫ޔ‬㘑๧ߩะ਄ലᨐ߇⹺߼ࠄࠇߚ‫ޕ‬ᾫᚑㅜਛߩ♧⚵ᚑߩᄌൻߦߟ޿ߡ߽
ႎ๔ߔࠆ‫ޕ‬− 64 −

10) ⊒⴫ቇળ㧦╙ 57 ࿁ᣣᧄ㘩ຠ⑼ቇᎿቇળᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 2 ᣣ‫᧲ޔ‬੩ㄘᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦࡟࠾ࡦᵴᕈߦ෸߷ߔࠨࡐ࠾ࡦ㘃ߩᓇ㗀ߦߟ޿ߡ㧦᭴ㅧᯏ⢻⋧㑐⸃ᨆ⊒⴫⠪㧦㜞ᯅ⍾❱‫ޔ‬ၳ ৻ਯ‫⟤⧛଻ޔ‬૫‫ޔ‬ᓟ⮮ ⁴㧝
㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯‫ޔ‬㧝⑺↰ᄢቇᎿቇ⾗Ḯቇㇱ㧕‫੃ູޔߪ♽ࡦࠪࡦ࠹ࠝࠡࡦࠕ࡮ࡦ࠾࡟ޤ⊛⋡ޣ‬㘃ߢᦨ߽㊀ⷐߥⴊ࿶⺞▵♽ߢ޽ࠆ‫ߩߘޕ‬ਛߢ߽࡟࠾ࡦߪᓞㅦ㉂⚛ߣߒߡⷐߩ૏⟎ࠍභ߼ߡ޿ࠆ‫ⴊᧄޔߢ߹ࠇߎޕ‬࿶⺞▵♽ߩ೙ᓮࠍ⋡ᜰߒߡ‫ࡦࠕޔ‬ࠡࠝ࠹ࡦࠪࡦᄌ឵㉂⚛ࠍ࠲࡯ࠥ࠶࠻ߣߒߚ㘩‛↱᧪㒖ኂ‛⾰ߩត⚝߇ⴕࠊࠇߡ᧪ߚ‫߇ߥߒ߆ߒޕ‬ࠄ‫ߦࡦ࠾࡟ޔ‬㑐ߒߡߪ㉂⚛౉ᚻ߿ᵴᕈ᷹ቯߩᾘ㔀ߐߥߤ߆ࠄ‫ޔ‬㘩‛↱᧪㒖ኂ‛⾰ߩត⚝ߪᱴߤⴕࠊࠇߡ޿ߥ߆ߞߚ‫⎇ᧄޕ‬ⓥߢߪ‫࡮ࠬ࡞ࠗ࠙ࡠࡘࠠࡃޔ‬Sf-9 ᣸⯻⚦⢩ၭ㙃♽ࠍ↪޿ߡ⊒⃻ߒߚࡅ࠻⚵឵߃ဳ࡟࠾ࡦ෸߮ߘߩ․⇣⊛ၮ⾰ࠍ↪޿ߡ㘩‛↱᧪࡟࠾ࡦ㒖ኂ‛⾰ត⚝♽ࠍ᭴▽ߒߚ‫ޔߚ߹ޕ‬࠳ࠗ࠭ࠃࠅ⷗಴ߐࠇߚ࡟࠾ࡦ㒖ኂ‛⾰ߢ޽ࠆ࠰ࡗࠨࡐ࠾ࡦ㧵ࠍၮᧄߣߒ‫ޔ‬ฦ⒳ࠨࡐ࠾ࡦ㘃ߦࠃࠆ࡟࠾ࡦ㒖ኂᵴᕈࠍᬌ⸛ߒ‫ޔ‬᭴ㅧᯏ⢻⋧㑐ߦߟ޿ߡᬌ⸛ࠍⴕߞߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ࡦ࠾࡟ࡠࡊ࡟ࡊ࠻ࡅޤ‬ㆮવሶࠍዉ౉ߒߚ⚵឵߃ဳࡃࠠࡘࡠ࠙ࠗ࡞ࠬࠍ Sf-9 ᣸⯻⚦⢩ߦᄙ㊀ᗵᨴߐߖ‫⚵ޔ‬឵߃ဳࡅ࠻࡟࠾ࡦࠍၭ࿾ਛߦ⊒⃻ߐߖߚ‫ޕ‬ᵴᕈဳ࡟࠾ࡦߩ↢↥߇⏕⹺ߐࠇߚၭ㙃ᦨᓟᦼߩၭ࿾ࠍ↪޿ߡ㧞Ბ㓏ߩࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ߦࠃࠅ࡟࠾ࡦࠍ♖⵾ߒߚ‫ࡦ࠾࡟ޕ‬ᵴᕈ᷹ቯߦߪ
ᣂⷙวᚑၮ⾰(Nma-Ile-His-Pro-Phe-His-Leu*Val-Ile-Thr-Lys(Dnp)-D-Arg-D-Arg-NH2)ࠍ↪޿ߚ‫ޕ‬‫⚿ޣ‬ᨐ‫ޤ‬๧ཬ↱᧪ߩ࡟࠾ࡦ㒖ኂ‛⾰ߩត⚝ߦࠃࠅ‫ߦ࠭ࠗ࠳ޔ‬㒖ኂ‛⾰ߩሽ࿷ࠍ⷗಴ߒ‫ߩߘޔ‬᭴ㅧࠍ࠰ࡗࠨࡐ࠾ࡦ I ߣหቯߒߚ‫ޔߢߎߘޕ‬ฦ⒳ࠨࡐ࠾ࡦ㘃ߦࠃࠆ࡟࠾ࡦ㒖ኂࠍᬌ⸛ߒߚ‫ޕ‬㒖ኂᬌቯ↪ࠨࡐ࠾ࡦ߅ࠃ߮㑐ㅪ‛⾰ߣߒߡߪ‫࠭ࠗ࠳ޔ‬㧔࠰ࡗࠨࡐ࠾ࡦ I ෸߮ II‫࡞࡯ࡁࠥࡐࠨࡗ࠰ޔ‬㧮㧕‫↞ޔ‬⨲㧔ࠣ࡝࠴࡞࡝࠴ࡦ㉄‫㉄ࡦ࠴࡟࡞࠴࡝ࠣޔ‬㧕‫ޔ‬ᩊ⢫㧔ࠨࠗࠦࠨࡐ࠾ࡦ B2 ෸߮㨏㧕‫ੱ▵┻ޔ‬ෳ㧔࠴ࠢ࠮࠷ࠨࡐ࠾ࡦ㘃㧕‫ੱ↪⮎ޔ‬ෳ㧔ࠡࡦ࠮ࡁࠨࠗ࠼㘃㧕߿ࡎࠝࠠࠡᨐታ↱᧪ࠨࡐ࠾ࡦ㧔ࡕࡕ࡞࠺ࠖࡦ㘃㧕╬ࠍ↪޿ߚ‫⚿ߩߘޕ‬ᨐ‫ ࡦ࠾ࡐࠨࡗ࠰ޔ‬I࡮II‫㉄ࡦ࠴࡝࡞࠴࡝ࠣޔ‬෸߮ࡕࡕ࡞࠺ࠖࡦ㘃ߦߪ࡟࠾ࡦ㒖ኂᵴᕈ߇⹺߼ࠄࠇߚ‫৻ޕ‬ᣇ‫࡞࡯ࡁࠥࡐࠨޔ‬㘃‫ޔ‬ᩊ⢫߿⮎↪ੱෳ↱᧪ߩࠨࡐ࠾ࡦ㘃ߦߪ࡟࠾ࡦ㒖ኂ߇⹺߼ࠄࠇߥ߆ߞߚ‫ޕ‬એ਄ߩ⚿ᨐ෸߮ࠨࡐ࠾ࡦ㘃ߩ᭴ㅧࠍൊ᩺ߔࠆߣ‫ߩࡦ࠾ࠥޔ‬㧟૏ߦࠣ࡞ࠢࡠ
ࡦ㉄߇⚿วߒߚࠣ࡞ࠢࡠ࠾࠼ࠨࡐ࠾ࡦ߇࡟࠾ࡦ㒖ኂࠨࡐ࠾ࡦߢ޽ࠆߎߣ߇␜ߐࠇߚ‫ޕ‬11) ⊒⴫ቇળ㧦ᣣᧄ㘩ຠ⑼ቇᎿቇળ╙ 57 ࿁ᄢળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 2 ᣣ‫᧲ޔ‬੩ㄘᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦ૐࠣ࡞࠹࡝ࡦ☨ߩ․ᓽߦߟ޿ߡ
⊒⴫⠪㧦ᄢ⢻ବਭ‫ޔ‬ᚭ᧻⺈‫ޔ‬㜞⇗⡡‫ޔ‬Ⴆᧄ⎇৻㧔⑺↰⋵✚ว㘩ຠ⎇㧕‫ޤ⊛⋡ޣ‬ૐࠣ࡞࠹࡝ࡦ☨ߪ‫ޔ‬ᓥ᧪ߩຠ⒳ߣߪ࠲ࡦࡄࠢ⾰ߩ᭴ᚑ߇⇣ߥࠅ‫߇ࡦ࡝࠹࡞ࠣޔ‬ዋߥߊࡊࡠ࡜ࡒࡦ߇ᄙ޿ߣߐࠇࠆ‫ߩ⾰ࠢࡄࡦ࠲ޕ‬᭴ᚑᲧ߇․ᱶߥߎߣ߆ࠄ‫ޔ‬ૐࠣ࡞࠹࡝ࡦ☨ߪ☨㘵࠹ࠢࠬ− 65 −

࠴ࡖ࡯╬߇․ᱶߥᕈ⁁ࠍ␜ߔน⢻ᕈ߇޽ࠆ‫ߢߎߘޕ‬ૐࠣ࡞࠹࡝ࡦ☨ߩ☨㘵࠹ࠢࠬ࠴ࡖ࡯╬ࠍ᣿ࠄ߆ߦߔࠆߴߊ⎇ⓥࠍⴕߞߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ޤ‬ኻᾖຠ⒳ߣߒߡ޽߈ߚߎ߹ߜ‫ޔࡦ࡯ࠗࠢ࡯ࠠ࡞ࡒޔ‬ૐࠣ࡞࠹࡝ࡦ☨ߣߒߡᤐ㓁‫ޔ‬LGC ࠰ࡈ࠻ࠍ⹜ᢱߣߒߚ㧔޿ߕࠇ߽⑺↰⋵↥㧕‫ ߦ☳☨ޕ‬SDS‫ޔ‬ዩ⚛‫࡞࡯ࡁ࠲ࠛ࠻ࡊࠞ࡞ࡔޔࡦ࡝࠮࡝ࠣޔ‬ࠍ฽߻ṁᶧࠍട߃ߡ࠲ࡦࡄࠢ⾰ࠍ᛽಴ߒ‫ߩߘޔ‬㆙ᔃ਄ᷡࠍ SDS-PAGE ߦଏߒߚ‫ࠬ࡯ࡠࡒࠕޕ‬㊂ߪᲧ⦡ߦࠃࠆ Juliano ߩ◲ᤃቯ㊂ᴺߢⴕߞߚ‫♟ޕ‬ൻ․ᕈ⹜㛎ߪ࡜ࡇ࠶࠼ࡆࠬࠦࠕ࠽࡜ࠗࠩ࡯ߢⴕߞߚ‫ޕ‬☨㘵࠹ࠢࠬ࠴ࡖ࡯ߩ᷹ቯߪ‫ࠍ☨ޔ‬᳓ᵞ޿ߒߚᓟ☨ߦኻߒߡ⫳⇐᳓ࠍ 1.6 ୚㊂ട߃ߡἺ㘵ߒ‫ࡦ࠹ޔ‬ࠪࡊ࡟࠶ࠨ࡯ߢ⎬ߐߣ☼ࠅࠍ᷹ቯߒ‫ࠬࡦ࡜ࡃޔ‬ᐲ㧔☼ࠅ㧛⎬ߐ㧕ࠍ▚಴ߔࠆߎߣߢⴕߞߚ‫ޕ‬‫⚿ޣ‬ᨐ‫ޤ‬ᤐ㓁‫ޔ‬LGC ࠰ࡈ࠻ߪ‫ޔ‬ኻᾖຠ⒳ߦᲧߴߡࠣ࡞࠹࡝ࡦ߇ዋߥߊ‫߇ࡦࡒ࡜ࡠࡊޔ‬ᄙ޿ߎߣࠍ
SDS-PAGE ߦࠃࠅ⏕⹺ߒߚ‫ࡠࡒࠕޕ‬㧙ࠬ㊂ߪ‫ ߇ߜ߹ߎߚ߈޽ޔ‬19.8%‫ ߇ࡦ࡯ࠗࠢ࡯ࠠ࡞ࡒޔ‬14.8%‫ޔ‬ᤐ㓁߇ 25.5%‫ޔ‬LGC ࠰ࡈ࠻߇ 17.9%ߢ޽ߞߚ‫☨ޕ‬㘵࠹ࠢࠬ࠴ࡖ࡯ߩࡃ࡜ࡦࠬᐲߪ‫ߜ߹ߎߚ߈޽ޔ‬߇ 0.315‫ ߇ࡦ࡯ࠗࠢ࡯ࠠ࡞ࡒޔ‬0.380 ߣ㜞߆ߞߚߩߦኻߒߡ‫ޔ‬ᤐ㓁ߪ 0.263‫ޔ‬LGC ࠰ࡈ࠻ߪ 0.264ߢ޽ࠅ‫ޔ‬ૐࠣ࡞࠹࡝ࡦ☨ߪૐ޿௑ะࠍ␜ߒߚ‫ޕ‬12) ⊒⴫ቇળ㧦╙ 10 ࿁㘩ຠ㉂⚛ൻቇ⎇ⓥળቇⴚ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 4 ᣣ‫ޔ‬ᄢ㒋ᐭ┙ᄢቇ㧔ᄢ㒋ᐭ႓Ꮢ㧕Ṷ㗴ฬ㧦ࡃࠠࡘࡠ࠙ࠗ࡞ࠬ࡮᣸⯻⚦⢩ߦ߅ߌࠆࡅ࠻࡟࠾ࡦߩല₸⊛↢↥ߣߘࠇࠍ↪޿ߚ㒖ኂ‛⾰ត⚝ߦߟ޿ߡ⊒⴫⠪㧦㜞ᯅ⍾❱㧝‫ޔ‬ၳ ৻ਯ㧝㧘቟๺ᐢਫ㧞‫⩵ޔ‬࿾⾫৻㧞‫ޔ‬ᓟ⮮ ⁴㧞
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞⑺↰ᄢ࡮Ꮏቇ⾗Ḯ㧕‫(♽ࡦࠪࡦ࠹ࠝࠡࡦࠕ࡮ࡦ࠾࡟ޤ⊛⋡ޣ‬RAS)ߪ‫⸃߽ᦨޔ‬ᨆ߇ㅴࠎߢ޿ࠆⴊ࿶⺞▵ᯏ᭴ߩ৻ߟߢ޽ࠆ‫ޕ‬࡟࠾ࡦߪ‫․ޔ‬ᕈߩ㜞޿ࠕࠬࡄ࡞࠹ࠖ࠶ࠢࡊࡠ࠹ࠕ࡯࠯ߢ‫ޔ‬ਥߦ⣢⤳ߩற♻⃿૕⚦⢩ߢวᚑߐࠇ‫ޔ‬⒳‫ߦޘ‬ೝỗߢⴊਛߦ᡼಴ߐࠇࠆ‫ࠍࡦࠥ࡯ࡁࡦࠪࡦ࠹ࠝࠡࡦࠕޔߪࡦ࠾࡟ޕ‬໑৻ߩၮ⾰ߣߒ‫ޔ‬ਇᵴᕈ࠺ࠞࡍࡊ࠴࠼ߢ޽ࠆࠕࡦࠡࠝ࠹ࡦࠪࡦ㧵(AI)ࠍㆆ㔌ߐߖࠆ‫ ߚߓ↢ޕ‬AI ߪ‫ࡦࠪࡦ࠹ࠝࠡࡦࠕޔ‬ᄌ឵㉂⚛(ACE)ߦࠃࠅ㧯ᧃ┵㧞ᱷၮ߇ಾ㒰ߐࠇᵴᕈဳߩࠕࡦࠡࠝ࠹ࡦࠪࡦ II(AII)ߣߥࠆ‫ޕ‬AII ߪ‫ޔ‬᭽‫ޘ‬ߥ♽ࠍ੺ߒߡⴊ࿶ࠍ਄᣹ߐߖࠆߎߣ߇⍮ࠄࠇߡ޿ࠆ‫ޔߢ߹ࠇߎޕ‬ᵴᕈ᷹ቯ߇ኈᤃߥ ACE ࠍ࠲࡯ࠥ࠶࠻ߣߒߡฦ⒳㘩᧚ࠃࠅ㒖ኂ‛⾰ߩត⚝߇ⴕࠊࠇߡ߈ߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬RAS ߩᓞㅦ㉂⚛ߢ޽ࠆ࡟࠾ࡦߦ㑐ߒߡߪ‫ޔ‬ᵴᕈ᷹ቯᴺߩᾘ㔀ߐߥߤߦࠃࠅ‫ޔ‬㘩‛↱᧪ߩ㒖ኂ‛⾰ត⚝⎇ⓥߪᱴߤⴕࠊࠇ
ߡ᧪ߥ߆ߞߚ‫⎇ᧄޔߢߎߘޕ‬ⓥߢߪ‫⚵ޔ‬឵߃ဳࡅ࠻࡟࠾ࡦߩ᣸⯻⚦⢩ߦ߅ߌࠆ⊒⃻♽ߩ᭴▽෸߮ࡊࡠ࡟࠾ࡦߩᵴᕈൻ㉂⚛ (PPE) ߩ⸃ᨆࠍⴕߞߚ‫⚵ߦࠄߐޕ‬឵߃ဳ࡟࠾ࡦࠍ↪޿ߚ㒖ኂ‛⾰ត⚝♽ࠍ᭴▽ߔࠆߣߣ߽ߦ‫ޔ‬ᄢ⼺↱᧪࡟࠾ࡦ㒖ኂ‛⾰ߩ᭴ㅧࠍ᣿ࠄ߆ߦߔࠆߣߣ߽ߦ‫ޔ‬᭴ㅧᯏ⢻⋧㑐ߦߟ޿ߡ߽ᬌ⸛ࠍⴕߞߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ࠍࡦ࠾࡟ࡠࡊ࡟ࡊ࠻ࡅޤ‬ዉ౉ߒߚ⚵឵߃ࡃࠠࡘࡠ࠙ࠗ࡞ࠬࠍ Sf-9 ᣸⯻⚦⢩ߦᗵᨴߐߖ࡟࠾ࡦ ߩ ⊒ ⃻ ࠍ ⴕ ߞ ߚ ‫ ߮ ࠃ ߅ ࡦ ࠾ ࡟ ࠻ ࡅ ޕ‬PPE ߩ ᷹ ቯ ߦ ߪ ‫ Ⱟ ޔ‬శ ᶖ శ ၮ ⾰(Nma-Ile-His-Pro-Phe-His-Leu*Val-Ile-Thr-Lys(Dnp)-D-Agr-D-Arg-NH2) ߅ ࠃ ߮ (Nma-Leu-Thr*LeuGly-Asn-Lys(Dnp)-D-Agr-D-Arg-NH2)㧔*, ၮ⾰ಾᢿㇱ૏‫ޔ‬㧔ᩣ㧕ࡍࡊ࠴࠼⎇ⓥᚲߦߡଐ㗬วᚑ㧕ࠍ↪
޿ߚ‫ޕ‬‫⚿ޣ‬ᨐ‫ࠍࡦ࠾࡟ࡠࡊ࡟ࡊ࠻ࡅޤ‬ዉ౉ߒߚ⚵឵߃ࡃࠠࡘࡠ࠙ࠗ࡞ࠬࠍᗵᨴߒߚ᣸⯻⚦⢩ߦ߅޿ߡߪ‫ޔ‬− 66 −

ၭ㙃ೋᦼߦࡊࡠ࡟࠾ࡦߩ⊒⃻߇ⷰኤߐࠇߚ‫ޔߚ߹ޕ‬ᗵᨴਛᦼ߆ࠄ PPE ߩ⊒⃻߇⏕⹺ߐࠇ‫ޔ‬ᗵᨴᓟᦼߩၭ࿾ਛߦߪ PPE ߩ૞↪ߦࠃࠅᵴᕈဳ࡟࠾ࡦߩ⫾Ⓧߔࠆߎߣࠍ⷗಴ߒߚ‫৻ޕ‬ᣇ‫⚵ޔ‬឵߃ဳࡅ࠻
࡟࠾ࡦࠍ࠲࡯ࠥ࠶࠻ߣߒߡᄢ⼺ࠃࠅ࡟࠾ࡦ㒖ኂ‛⾰♖⵾ߒ‫ߩߘޔ‬᭴ㅧࠍ࠰ࡗࠨࡐ࠾ࡦ㧵ߣหቯߒߚ‫ޔߦࠄߐޕ‬ฦ⒳ࠨࡐ࠾ࡦ㘃ࠍ↪޿ߡ࡟࠾ࡦ㒖ኂᵴᕈࠍᬌ⸛ߒߚ⚿ᨐ‫߇ࡦ࠾ࡐࠨ࠼࠾ࡠࠢ࡞ࠣޔ‬࡟࠾ࡦ㒖ኂࠨࡐ࠾ࡦߢ޽ࠆߎߣࠍ᣿ࠄ߆ߣߒߚ‫ޕ‬‫ޣ‬ෳ⠨⺰ᢥ‫ޤ‬
1. Takahashi, S., et al. Biosci. Biotechnol. Biochem., 71, 2610-2613 (2007)
2. Takahashi, S., et al. ibid., 72, 3232-3236 (2008)
3. Gotoh, T., et al. ibid., 74, 370-374 (2010)13) ⊒⴫ቇળ䋺↢૕කᎿቇ䉲䊮䊘䉳䉡䊛 㪉㪇㪈㪇㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ 㪐 ᦬ 㪈㪇 ᣣ䇮ർᶏ㆏ᄢቇ㩷Ṷ㗴ฬ䋺㪜㪩㪧 䉕ᜰᮡ䈫䈚䈢㘩ຠ⹏ଔ䈮㑐䈜䉎ታ㛎⊛ᬌ⸛㩷 㵩㩷 ↉䉕଀䈫䈚䈢ᄖⷰ⹏ଔ㵩㩷⊒⴫⠪䋺䂾↰ਛరᔒ㵴㩷 䇮ᧄ㑆ᥓᄢ㵴䇮㩷 ੗਄㩷 ᶈ㵴㩷 䇮ᣂጊ༑༹㵵䇮㩷 㜞ᯅ㩷 ᔀ㺒䇮㩷 ᾢ⼱᣽ೣ㺒䇮㩷 㩷⑺ጊ⟤ዷ㺒㺒㩷
㩿㵴⑺↰ᄢቇᄢቇ㒮Ꮏቇ⾗Ḯቇ⎇ⓥ⑼䇮㵵⑺↰ᄢᄢቇ㒮කቇ♽⎇ⓥ⑼䇮㺒⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷㺒㺒⑺↰⋵┙ᄢቇ↢‛⾗Ḯ⑼ቇㇱ㪀㩷੐⽎㑐ㅪ㔚૏䋨㪜㪩㪧䋩䉕ᜰᮡ䈫䈚䈢㘩ຠ䈱⹏ଔᴺ䉕ᬌ⸛䈜䉎䈢䉄䈮䇮ᒻ⁁䇮⦡䈭䈬ᄖⷰ䈏⇣䈭䉎㘶䉂‛䈱↉䉕଀䈫䈚䈩䇮㪍 ᨎ䈱౮⌀䉕 㪋 Ბ㓏䈱⹏ଔ⺆䈪ᅢ䉂⹏ଔ䈚䈢䈫䈐䈱 㪜㪩㪧 䉕᷹ቯ䈚䈢䇯䈠䈱⚿ᨐ䇮䇸䈫䈩䉅ᅢ䈐䇹䈫⹏ଔ䈚䈢䈫䈐䈮 㪧㪊㪇㪇 㕙Ⓧ䋨ẜᤨ 㪉㪌㪇䌾㪌㪇㪇㫄㫊䋩䈏ᦨ䉅ᄢ䈐䈒䈭䈦䈢䇯ઁ䈱⹏ଔ⺆䈪䈱㆑䈇䈲ዊ䈘䈎䈦䈢䈏䇮䈫䈩䉅ᅢ䈐䈭‛䉕ᬌ಴䈪䈐䉎น⢻ᕈ䈲ᓧ䉌䉏䈢䇯㩷㩷㩷 ⊒⴫ቇળ㧦 ᣣᧄᗵᕈᎿቇળᐕᰴᄢળ
⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ ᧲੩Ꮏᬺᄢቇ㧔᧲੩ㇺ㧕Ṷ㗴ฬ㧦࿾ၞ․↥ຠ㐿⊒ߦ߅ߌࠆᗵᕈࡑ࡯ࠤ࠹ࠖࡦࠣᚻᴺߩ೑↪ߩᬌ⸛⊒⴫⠪㧦٤㜞⇗ ⡡ 㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧕㧝㧚ߪߓ߼ߦ ࠨ࡯ࡆࠬࠍ฽߼ߚᐢ޿ᗧ๧ߢߩ⑺↰⋵࿾ၞ․↥ຠߩ㜞ઃടଔ୯ൻߩ⹜ߺߣߒߡ‫ޔ‬‫⚻ޟ‬㛎ଔ୯ࡑ࡯ࠤ࠹
ࠖࡦࠣ‫ߩ╬ޠ‬ᗵᕈࡑ࡯ࠤ࠹ࠖࡦࠣᚻߩ೑↪ࠍᬌ⸛ߒߚ‫ޕ‬㧞㧚‫ޟ‬ᗵᕈଔ୯ߠߊࠅ‫ࠣࡦࠖ࠹ࠤ࡯ࡑࠆࠃߦޠ‬ᚻᴺߦ߅ߌࠆ࿾ၞ․↥ຠ╬ߩ㜞ઃടଔ୯ൻ
‫ޟ‬ᗵᕈଔ୯ߠߊࠅ‫ࠣࡦࠖ࠹ࠤ࡯ࡑࠆࠃߦޠ‬ᚻᴺߣߪ‫↢ޔ‬ᵴ⠪ߩᗵᕈߦ௛߈߆ߌ‫౒ޔ‬ᗵ࡮ᗵേࠍᓧࠆ
ߎߣߢ㗼࿷ൻߔࠆଔ୯ߢ޽ࠆ‫ޟ‬ᗵᕈଔ୯‫ߦޠ‬ᵈ⋡ߒ‫ࠍࠇߎޔ‬೑↪ߒࠦࡦ࠮ࡊ࠻᭴▽߿໡ຠ⸳⸘╬ࠍⴕ߁ࡑ࡯ࠤ࠹ࠖࡦࠣᚻᴺߢ޽ࠆ‫ౕޕ‬૕⊛ߦߪ‫ޟޔ‬ᭉߒߐ‫ޟޔޠ‬቟ᔃᗵ‫ޟޔޠߐࠃߎߞ߆ޟޔޠ‬૞ࠅᚻ߳ߩ౒ᗵ‫ⷙޔߤߥޠ‬ᩰ߿ࠬࡍ࠶ࠢ╬ߢߪ⴫⃻ߒߦߊ޿ⷐ⚛ࠍઃടଔ୯ߣߒߡઁ໡ຠߣߩᏅ೎ൻ╬ߦ೑↪
ߔࠆߥߤ߇⠨߃ࠄࠇࠆ‫ߩ↰⑺ޕ‬࿾ၞ․↥ຠߩ໡ຠࠦࡦ࠮ࡊ࠻᭴▽ߦ‫ޟߩߎޔ‬ᗵᕈଔ୯ߠߊࠅ‫ࠆࠃߦޠ‬ࡑ࡯ࠤ࠹ࠖࡦࠣᚻᴺࠍᗵᕈࡑ࡯ࠤ࠹ࠖࡦࠣᚻᴺߩ߭ߣߟߣߒߡ‫ߩߘޔ‬೑↪ࠍᬌ⸛ߒߚ‫ޕ‬੹࿁ߩ⎇ⓥߢߪ‫ߦ․ޔ‬ᐢ޿ᗧ๧ߢߩ࿾ၞ․↥ຠߢ޽ࠆኋᴱᣉ⸳ߩ㘶㘩ࠨ࡯ࡆࠬߩᡷༀ‫ޔ‬ะ਄ߦࠃࠆ

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࿾ၞ․↥ຠ㧔ࠨ࡯ࡆࠬ㧕ߩ㜞ઃടଔ୯ൻ߳ߩ೑↪ߦߟ޿ߡ߽ᬌ⸛ߒߚ‫ޕ‬⴫㧝 ⑺↰⋵ኋᴱᣉ⸳ߦ߅ߌࠆᦺ㘩ߩ⚻㛎ଔ୯଀ಽ㘃㧿㧱㧺㧯㧱

⑺↰⋵ኋᴱᣉ⸳ߦ߅ߌࠆᦺ㘩ߩ⚻㛎ଔ୯଀Ἲ߈ߚߡߩߏ㘵ߩ㚅ࠅ⧐㉍ߥߺߘ᳝ߩ㚅ࠅࠪࡖࠠࠪࡖࠠߣߒߚẃߌ‛ߩ㘩ᗵ  ߥߤ㧲㧱㧱㧸☨↥࿾ߥࠄߢߪߩ㒢ቯຠ⒳ߩ☨㘵↢↥⠪߇ࠊ߆ࠆේᢱ☨‫ޔ‬㊁⩿‫ޔ‬㝼╬߳ߩ቟ᔃᗵ ‫⥄ޟ‬ὼ⼾߆ߥ☨ߤߎࠈ࡮޽߈ߚ‫ߩ߳ޠ‬ㇹᗜ ߥߤ㨀㧴㧵㧺㧷

ૐࠕࡒࡠ࡯ࠬ☨‫ޔ‬ኙ಄࿾ㆡ૞‫⋥ޔ‬᠞߈ᩱၭ╬ߩ☨↥࿾ߥࠄߢߪߩળ⹤߆ࠄᓧࠄࠇࠆ߁
ࠎߜߊਛጊ㑆࿾╬ߢᩱၭߢ߈ࠆ࿾ၞ․↥ຠ⒳ࠍ㘩ߔࠆߎߣ߆ࠄᓧࠄࠇࠆ␠ળ⊛ᵴേᗧ⟵ ߥ
ߤ

㧭㧯㨀

ᢥൻߩ߭ߣߟߢ޽ࠆ࿾ၞߢߩ㘩ࠍ૕㛎ߔࠆߎߣߦࠃࠆ࡜ࠗࡈࠬ࠲ࠗ࡞ߩᏅ೎ൻߩ⥄ᗧ⼂ ߥߤ

㧾㧱㧸㧭㨀㧱 વ⛔⊛㘩ᢥൻߢ޽ࠆ⑺↰ߩᦺ㘩ࠍ૕㛎ߒ‫ߦޘੱߩઁޔ‬વ߃ࠆߎߣߩ಴᧪ࠆ༑߮‫ޔ‬ḩ⿷ᗵ ߥߤ
㧠㧚߹ߣ߼ ‫⚻ޟ‬㛎ଔ୯⸳⸘‫ޠ‬ᚻᴺ߅ࠃ߮‫ޟ‬ᗵᕈଔ୯ߠߊࠅ‫ࠣࡦࠖ࠹ࠤ࡯ࡑࠆࠃߦޠ‬ᚻᴺߦࠃࠅ೑↪ߦࠃࠆ࿾ၞ
․↥ຠߩ㜞ઃടଔ୯ൻߦߟ޿ߡᬌ⸛ߒߚ‫⚻ޕ‬㛎ଔ୯ࡕࠫࡘ࡯࡞߿ᗵᕈଔ୯ࠍࡈ࡟࡯ࡓࡢ࡯ࠢߣߒ‫ޔ‬࿾ၞ․↥ຠ߿ࠨ࡯ࡆࠬߩᜬߞߡ޿ࠆᣢሽଔ୯ߩ⹏ଔⷞὐ╬ࠍᄌ߃ࠆߎߣߦࠃࠅ‫ࠅࠃޔ‬㝯ജ⊛ߥ࿾ၞ․↥ຠ߿ࠨ࡯ࡆࠬߦߔࠆߎߣ߇಴᧪ࠆน⢻ᕈ߇޽ࠆߎߣ߇ࠊ߆ߞߚ‫ޕ‬15) ⊒⴫ቇળ䋺╙ 㪌㪎 ࿁ᣣᧄᩕ㙃ᡷༀቇળቇⴚ✚ળ㩷⊒⴫ᣣ䈫႐ᚲ䋺㪉㪇㪈㪇 ᐕ 㪐 ᦬ 㪈㪈 ᣣ䇮ᅚሶᩕ㙃ᄢቇ䋨ၯ₹⋵ဈᚭᏒ䋩㩷Ṷ㗴ฬ䋺䉺䊮䊌䉪⾰ടᎿ㘩ຠ䈏᭽䇱䈭ṁᇦ䈱๧⾰䈮෸䈿䈜ᓇ㗀䈮㑐䈜䉎ቴⷰ⊛⹏ଔ㩷⊒⴫⠪䋺䂾↰┵㩷 ብల䋱䋩䇮ᾢ⼱㩷 ᣽ೣ䋲䋩䇮ศ↰㩷 ᥓ䋳䋩䇮㋈ᧁ㩷 㕏ᔒ䋱䋩㩷䋨䋱䋩䉰䊤䊟ᩣᑼળ␠㩷 䊋䉟䉥䉬䊚䉦䊦⎇ⓥᚲ䇮䋲䋩⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷䋳䋩䇮᧲੩䉰䊤䊟ᩣᑼળ␠㩷ᩕ㙃䉬䉝ផㅴቶ䋩㩷䇼⋡⊛䇽㜞㦂⠪䈪䈲ૐᩕ㙃⁁ᘒ䈱੍㒐䉇ᡷༀ䈱䈢䉄䈮᭽䇱䈭ᩕ㙃⵬ഥ㘩ຠ䉕↪䈇䈢ᩕ㙃੺౉䈏ⴕ䉒䉏䉎䈖䈫䈏ᄙ䈇䇯䈭䈎䈪䉅䇮☳ᧃ⁁䉺䊮䊌䉪⾰ടᎿ㘩ຠ䋨䉺䊮䊌䉪㘩ຠ䋩䈲ᚻシ䈮䉺䊮䊌䉪䉇ᓸ㊂ᩕ㙃⚛䈱⵬⛎䈏䈪䈐䉎䊆䊷䉵䈱㜞䈇ᩕ㙃⵬ഥ㘩ຠ䈪䈅䉎䇯䉺䊮䊌䉪㘩ຠ䈲๧ཬ᳝╬䈱㘩ຠ䈮ᷝട䈚䈩៨ข䈜䉎䈖䈫䈏ᄙ䈇䈢䉄䇮㘩ຠ䈱๧䉇‛ᕈ䈮ᓇ㗀䈚䈭䈇䉅䈱䈏ᦸ䉁䈚䈇䇯੹࿁䇮䉒䉏䉒䉏䈲๧ⷡ䉶䊮䉰䊷䉕↪䈇䈩䉺䊮䊌䉪㘩ຠ䉕ᷝട䈚䈢㘩ຠ䈱๧䉕᷹ቯ䈜䉎䈖䈫䈮䉋䉍䇮๧⾰ᄌൻ䈱ቴⷰ⊛䈭⹏ଔ䉕⹜䉂䈢䇯㩷䇼ᣇᴺ䇽Ꮢ⽼䈱䉺䊮䊌䉪㘩ຠ 㪊㩷 ⒳㘃䋨ᄢ⼺䊶੃ᷡ䉺䊮䊌䉪ᷙวဳ䋺㪘䇮ᄢ⼺䉺䊮䊌䉪ਥ૕ဳ䋺㪚䇮੃ᷡ䉺䊮䊌䉪ਥ૕ဳ䋺㪛䋩䈫䇮ᷙวဳ䋨㪘䋩䈎䉌䊎䉺䊚䊮䊶䊚䊈䊤䊦䉕㒰䈇䈢⹜ଏຠ䈱䉺䊮䊌䉪㘩ຠ䋨㪙㪀䈱ว⸘ 㪋㩷 ⒳㘃䉕䉺䊮䊌䉪⾰䈫䈚䈩৻ቯỚᐲ䈮䈭䉎䉋䈉䈮๧ཬ᳝䇮䈍♄䇮‐੃䇮੃㉄⩶㘶ᢱ䇮䉮䊮䉸䊜䉴䊷䊒䇮䈍⨥䈭䈬䈱ฦ㘩᧚

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䈮ᷝട䈚䈢⹜㛎ᶧ䉕↪䈇䈩䇮๧ⷡ䉶䊮䉰䊷䈪᷹ቯ䉕ⴕ䈦䈢䇯ᓧ䉌䉏䈢䉶䊮䉰䊷ᔕ╵୯䈮ኻ䈚䈩䊌䉺䊷䊮⹺⼂ಽ㘃䉕ㆡ↪䈚䇮๧⾰䈱ᄌൻ䉕ੑᰴర䈱๒๧䊙䉾䊒䈫䈚䈩⴫䈚䈢䇯㩷䇼⚿ᨐ䈫⠨ኤ䇽䊙䉾䊏䊮䉫䈱⚿ᨐ䇮㘩᧚㑆䈱๧⾰䈱Ꮕ䉕Ყセ䈜䉎䈫䉺䊮䊌䉪㘩ຠ䉕ᷝട䈚䈢䈖䈫䈮䉋䉎๧⾰ᄌൻ䈱ᐲว䈇䈲ዋ䈭䈇䈖䈫䈏␜ໂ䈘䉏䈢䇯․䈮‐੃䇮੃㉄⩶㘶ᢱ䇮๧ཬ᳝䈪䈲䉺䊮䊌䉪㘩ຠᷝട೨ᓟ䈱ಽᏓ䈏㘩᧚Ფ䈮㓸䉁䉍䇮䉺䊮䊌䉪㘩ຠ䈏๧⾰䈻෸䈿䈜ᓇ㗀䈲ዊ䈘䈎䈦䈢䇯䉮䊮䉸䊜䉴䊷䊒䈪䈲 㪛㩷 䈏⚛᧚䈱๧⾰䉕ᦨ䉅ᄢ䈐䈒ᄌ䈋䉎௑ะ䈏⷗䉌䉏䇮䊘䉺䊷䉳䊠䉴䊷䊒䈪䈲 㪘䇮㪙㩷 䈱㗅䈪ᄌൻ䈱ᐲว䈇䈏ዋ䈭䈒䇮㘩᧚䈮䉋䈦䈩䉺䊮䊌䉪㘩ຠ䈱ㆡ䊶ਇㆡ䈏⇣䈭䉎䈖䈫䈏␜ໂ䈘䉏䈢䇯䈍♄䈫䈍⨥䈲㘩᧚䈱๧⾰䊌䉺䊷䊮䈮ኻ䈚䈩䇮䉺䊮䊌䉪㘩ຠ䉕ᷝട䈚䈢ᤨ䈱ᄌൻ䈱ᐲว䈇䈏ᄢ䈐䈒䇮䈠䉏䉌䈻䈱ㆡᕈ䈲ቭ⢻⹜㛎䉅฽䉄䈩್ᢿ䈜䉎ᔅⷐ䈏䈅䉎䈫⠨䈋䉌䉏䉎䇯㩷㩷㩷16) ⊒⴫ቇળ㧦ᣣᧄᔕ↪♧⾰⑼ቇળᐔᚑ 22 ᐕᐲᄢળ㧔╙ 59 ࿁㧕⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 15 ᣣ‫ࡊ࠶ࠪࡦ࡜ࠣޔ‬㧔㕒ጟᏒ㧕㧦ੑ㊀ᄌ⇣૕☨⢦੃ố☳ߩ‛ℂൻቇ․ᕈ
Ṷ㗴ฬ㧦⊒⴫⠪㧦㜟ᯅᔀ 1, ቟᧲┥৻ 2, ⧎ၔൟ 3, ᾢ⼱᣽ೣ 1, ਛ᧛଻ౖ 4, ⮮↰⋥ሶ 4 (⑺↰✚㘩⎇ 1, ᣣᧄ㘩ຠൻᎿ 2, 㣮ఽፉᄢ࡮ㄘ 3, ⑺↰⋵┙ᄢ 4)‫ޤ⊛⋡ޣ‬ᚒ‫⎇ߩޘ‬ⓥࠣ࡞࡯ࡊߢߪố☳↢วᚑ㑐ㅪ㉂⚛߇ᰳ៊ߒߚࠗࡀߩ⢦੃ᄌ⇣૕☨ߩ੤㈩ߦࠃ
ࠆੑ㊀ᄌ⇣૕ࠍ૞಴ߒ㧘ᓧࠄࠇߚ☨ố☳ߩ᭴ㅧ߿‛ᕈ⹏ଔࠍၮߦ㉂⚛‫ޔ‬ố☳᭴ㅧ‫‛ޔ‬ᕈߩ㑐ଥࠍ᣿ࠄ߆ߦߔࠆߎߣߢ‫ޔ‬ố☳↢วᚑࡔࠞ࠾࠭ࡓߩో⽩⸃᣿ࠍ⋡ᮡߣߒߡ޿ࠆ.ᧄ⎇ⓥߢߪ㧘ੑ㊀ᄌ⇣૕☨ố☳ߩ‛ℂൻቇ․ᕈߦߟ޿ߡ⺞ߴߚ㧚‫ޣ‬ᣇᴺ‫ߦߢߔޤ‬න㔌ߐࠇߡ޿ࠆࠬ࠲࡯࠴ࠪࡦ࠲࡯࠯ SSIIIaᄌ⇣૕㧔SSIIIa㧕ࠍⷫߦߒߚࠗࡀੑ㊀ᄌ⇣૕߅ࠃ߮ߘࠇࠄߩⷫᄌ⇣૕♽⛔ߩ♖⵾☨ố☳ࠍಽᨆߦ↪޿ߚ㧚ฦố☳ߩ㎮㐳᭴ㅧ⸃ᨆ㧘ᒻᘒⷰኤ㧘☸ᐲಽᏓ᷹ቯ㧘RVA ߦࠃࠆ♟ൻ․ᕈࠍ᷹ቯߒߚ㧚
RVA ᷹ቯᓟߩ♟ᶧ㧔10wt%㧕ߩૐ᷷଻ᜬᤨߩ࡟ࠝࡠࠫ࡯․ᕈࠍേ⊛☼ᒢᕈ᷹ቯⵝ⟎ߦࠃߞߡ᷹ቯ
ߒߚ㧚‫⚿ޣ‬ᨐ‫ޤ‬SSIIIa ࠍⷫߦߒߚੑ㊀ᄌ⇣૕ߩố☳☸ߩᒻ⁁ߪਣߺࠍᏪ߮ߡ޿ߚ㧚ౖဳ⊛ߥᣣᧄဳ☨ߩ♟ൻ․ᕈᦛ✢ࠍ␜ߔᣣᧄ᥍ߦኻߒߡੑ㊀ᄌ⇣૕☨ố☳ߪ☼ᐲ਄᣹᷷ᐲ߇㜞ߊ㧘ടᾲᤨࡇ࡯ࠢ☼ᐲ߇㕖Ᏹߦૐ޿߆ࡇ࡯ࠢࠍᜬߚߕ㧘ࡉ࡟ࠗࠢ࠳࠙ࡦ߽ዊߐ޿․ᓽࠍ␜ߒ,⤘Ả㨯♟ൻߒߦߊ޿ố☳ߢ޽ࠆߣ⠨߃ࠄࠇߚ㧚RVA ᷹ቯ⋥ᓟߩᄌ⇣૕☨ố☳♟ᶧߪᵹേߒ߿ߔ߆ߞߚ߇㧘ૐ᷷଻ሽᤨߩᤨ㑆⚻ㆊߦߒߚ߇޿⾂⬿ᒢᕈ₸߇ᣣᧄ᥍ߩ⚂ 10㨪100 ୚߹ߢჇᄢߒߚ㧚ߎߩ⚿ᨐ߆ࠄ㧘ੑ㊀ᄌ⇣૕☨ố☳ߪ㊁↢ဳࠃࠅ߽⠧ൻ߇ㅦ޿ߎߣࠍ␜ໂߐࠇ㧘ߎࠇߪ․⇣⊛ߥ㎮㐳᭴ㅧߦ↱᧪ߔࠆߚ߼ߣ⠨߃ࠄࠇࠆ㧚ᧄ⎇ⓥߪࠗࡁࡌ࡯࡚ࠪࡦഃ಴ၮ␆⊛⎇ⓥផㅴ੐ᬺ㧔↢⎇࠮ࡦ࠲࡯㧕ߦߡታᣉߐࠇߚ㧚17) ⊒⴫ቇળ㧦╙ 6 ࿁ D-ࠕࡒࡁ㉄⎇ⓥળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 17 ᣣ‫ޔ‬ንጊ࿖㓙ળ⼏႐㧔ንጊᏒ㧕Ṷ㗴ฬ㧦ේᩭᓸ↢‛↱᧪ D-ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯㧔Paenidase㧕ㆮવሶߩࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯߆ࠄߩන㔌‫ޔ‬෸߮↢↥⩶ߩోࠥࡁࡓࠪ࡯ࠢࠛࡦࠬ
⊒⴫⠪㧦㖴Ỉᖗ㧝㧘㜞ᯅ⍾❱㧞㧔㧝࿖㓙ㄘ᳓⎇ⓥ࠮ࡦ࠲࡯‫ޔ‬㧞⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯㧕‫৻ޔߢ߹ࠇߎޤ⊛⋡ޣ‬ㇱߩ⚦⩶ߩ⚦⢩⤑ߦ D ဳࠕࡒࡁ㉄ߩሽ࿷ߔࠆߎߣ߇⍮ࠄࠇߡ޿ߚ߇‫ޔ‬ㄭᐕ‫ޔ‬− 69 −

ູ੃㘃ߩ↢૕ౝߦ߽ㆆ㔌ߩ D ဳࠕࡒࡁ㉄߿ D ဳࠕࡒࡁ㉄ࠍ฽᦭ߔࠆ࠲ࡦࡄࠢ⾰ߩሽ࿷ߔࠆߎߣ߇
⷗಴ߐࠇߡ޿ࠆ‫ ߩࠄࠇߎޔߦࠄߐޕ‬D ဳࠕࡒࡁ㉄߇᭽‫∛ߥޘ‬ᘒߣ㑐ㅪߔࠆߎߣ߇␜ໂߐࠇߡ޿ࠆ‫ޕ‬․ߦ‫ޔ‬D-ࠕࠬࡄ࡜ࠡࡦ㉄߇ᵈ⋡ߐࠇߡ߅ࠅ‫㉄ࡦࠡ࡜ࡄࠬࠕߪߢ⾰ࠢࡄࡦ࠲  ࠼ࠗࡠࡒࠕޔ‬ᱷၮߩD ဳᄌ⇣߇ࠕࡒࡠࠗ࠼࠲ࡦࡄࠢ⾰ߩಝ㓸ࠍᒁ߈⿠ߎߔߎߣ߇␜ໂߐࠇߡ޿ࠆ‫ޕ‬㜞ᯅࠄߪ D-ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯↢↥⩶㧔Paenibacillus sp. B38 ᩣ㧕ࠍಽ㔌ߔࠆߣߣ߽ߦ‫ࠆߔ↥↢ޔ‬㉂⚛ࠍ paenidase ߣ๮ฬߒ‫ߩߘޔ‬ᕈ⾰ࠍ᣿ࠄ߆ߦߒߚ㧔S. Takahashi et al., 㧶. Biochem, (2006㧕 139,
197-202㧕‫੹ޕ‬࿁ᚒ‫ޔߪޘ‬PCR ᴺߦࠃࠅขᓧߒߚ paenidase ㆮવሶߩႮၮ㈩೉ࠍౣ⏕⹺ߔࠆߚ߼ߦ‫ޔ‬ࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯ࠃࠅㆮવሶࠍන㔌ߒ‫ߩߘޔ‬㈩೉ࠍ⺞ߴߚ‫ޔߚ߹ޕ‬Paenibacillus sp. B38 ᩣߩ߽ߟ D ဳࠕࡒࡁ㉄㑐ㅪ㉂⚛ࠍ⸃ᨆߔࠆߚ߼‫ޕߚߞⴕࠍࠬࡦࠛࠢ࡯ࠪࡓࡁࠥోޔ‬‫ޣ‬ᣇᴺ‫ޤ‬Paenibacillus
sp. B38 ᩣࠃࠅන㔌ߒߚࠥࡁࡓ DNA ࠍ↪޿ߡ࡚ࠪ࠶࠻ࠟࡦ࡜ࠗࡉ࡜࡝࡯ࠍ૞ᚑߒ‫࡯࠾ࡠࠦޔ‬PCR
ᴺߦࠃࠅ⋡⊛㈩೉ࠍ฽߻ࠢࡠ࡯ࡦࠍࠬࠢ࡝࡯࠾ࡦࠣߒߚ‫ ߪߦࠬࡦࠛࠢ࡯ࠪࡓࡁࠥోޔߚ߹ޕ‬Roche␠⵾ Genome Sequencer FLX System ࠍ↪޿ߚ‫ޔߦࠄߐޕ‬Ⴎၮ㈩೉෸߮ࠕࡒࡁ㉄㈩೉ߩ⋧หᕈ⸃ᨆߪ
BLAST ࠍ‫ߩ࡯࡝ࡒࠔࡈ࠯࡯࠳࠴ࡊࡍޔ‬ಽ㘃ߪ MEROPS ࠺࡯࠲ࡌ࡯ࠬࠍ↪޿ߡⴕߞߚ‫ޕ‬‫⚿ޣ‬ᨐ෸߮
⠨ኤ‫⚿ߩࠣࡦ࠾࡯࡝ࠢࠬޤ‬ᨐ‫ޔ‬3,804bp ߩࠗࡦࠨ࡯࠻ࠍᜬߟࡊ࡜ࠬࡒ࠼㧔pUC118/K19㧕ࠍන㔌ߒߚ‫ޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ߩႮၮ㈩೉ࠍಽᨆߒ‫ޔ‬PCR ᴺߦࠃࠅᓧࠄࠇߚႮၮ㈩೉ߣᲧセߒߚߣߎࠈ‫ޔ‬⠡⸶㗔ၞߢߪႮၮߩ㆑޿ߪߥ߆ߞߚ߇‫ޔ‬5’஥ߩ㕖⠡⸶㗔ၞߢ㈩೉߇⚵ߺ឵ࠊߞߡ޿ߚ‫ޔߢߎߘޕ‬ౣᐲ 5’஥ߩ㕖⠡⸶㗔ၞࠍ฽߻ࠢࡠ࡯ࡦࠍࠬࠢ࡝࡯࠾ࡦࠣߒߚߣߎࠈ‫ޔ‬4,071bp ߩࠗࡦࠨ࡯࠻ࠍᜬߟ
ࡊ࡜ࠬࡒ࠼㧔pUC118/64㧕ࠍන㔌ߒߚ‫ޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ߩႮၮ㈩೉ࠍಽᨆߒ‫ޔ‬pUC118/K19 ߩႮၮ㈩೉ߣ⍦⋫߇ߥ޿ߎߣࠍ⏕⹺ߒߚ‫੹ޕ‬࿁⸃ᨆߒߚ㗔ၞߦߪ paenidase ㆮવሶࠍ฽߼ߡ᭴ㅧㆮવ
ሶ߇ 4 ⒳㘃ሽ࿷ߔࠆߣផቯߐࠇߚ‫ޕ‬Paenidase એᄖߩㆮવሶߪ‫ ࠇߙࠇߘޔ‬Clostridium cellulolyticum↱᧪ Cellulosome anchoring protein (30%)‫ޔ‬Brevibacillus brevis ↱᧪ ATPase ᭽࠲ࡦࡄࠢ⾰ (73%)‫ޔ‬ห↱᧪ 4-aminobutyrate aminotransferase ᭽࠲ࡦࡄࠢ⾰ (48%)ߣ⋧หᕈ߇޽ߞߚ‫ޕ‬ᰴߦ‫ޔ‬ᰴ਎ઍࠪ࡯ࠢ
ࠛࡦࠨ࡯ࠍ↪޿ߡ⸃ᨆߒߚࠥࡁࡓ DNA Ⴎၮ㈩೉ࠍ߽ߣߦ‫ޔ‬ฦ⒳࠺࡯࠲ࡌ࡯ࠬ⸃ᨆࠍⴕߞߚߣߎࠈ‫ޔ‬
Paenibacillus sp. B38 ᩣࠥࡁࡓߦߪࡍࡊ࠴࠳࡯࠯ࡈࠔࡒ࡝࡯S11 ෸߮ S12 ߦ⋧หᕈࠍ߽ߟ᭴ㅧㆮવ
ሶ߇ߘࠇߙࠇ 3 ⒳㘃෸߮ 7 ⒳㘃ሽ࿷ߔࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ㧔S12 ߩ 7 ⒳㘃ߦߪ Paenidase ࠍ฽߻㧕‫ޔߚ߹ޕ‬S13 ߦ⋧หᕈࠍ߽ߟ߽ߩߪߥ߆ߞߚ‫ޕ‬S11㨪13 ߩ 3 ⒳㘃ߩࡍࡊ࠴࠳࡯࠯ࡈࠔࡒ࡝࡯ߪ
ਥߦ D ဳࠕࡒࡁ㉄㑐ㅪ㉂⚛‫⚿ࡦ࡝ࠪ࠾ࡍޔ‬ว࠲ࡦࡄࠢ⾰ߦࠃࠅ᭴ᚑߐࠇߡ޿ࠆ‫ޕ‬Paenibacillus sp.
B38 ᩣߩ߽ߟߎࠇࠄ D ဳࠕࡒࡁ㉄㑐ㅪ㉂⚛ߦߟ޿ߡᲧセ⸃ᨆߔࠆߎߣߪ‫ޔ‬Paenidase ߩ․ᓽࠍ⸃᣿
ߔࠆ਄ߢ᦭ലߢ޽ࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬18) ⊒⴫ቇળ㧦╙ 6 ࿁ D-ࠕࡒࡁ㉄⎇ⓥળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 9 ᦬ 17 ᣣ‫ޔ‬ንጊ࿖㓙ળ⼏႐㧔ንጊᏒ㧕Ṷ㗴ฬ㧦⢖⢩਄⊹⚦⢩ߦ߅ߌࠆ Protein L-isoaspartyl/D-aspartyl methyltransferase ㆮવሶࡁ࠶ࠢ࠳࠙ࡦߪ epithelial-to-mesenchymal transition (EMT)ࠍ⺃ዉߔࠆ⊒⴫⠪㧦ዊ═ේᱜੱ㧝㧘ᚭ↰ᐕ✚㧞‫ޔ‬㊀ᧄ๺ብ㧞‫ޔ‬㖴Ỉᖗ㧟‫ޔ‬㜞ᯅ⍾❱㧠‫ޔ‬ጊౝᐢᐔ㧡㧔㧝ᗲᇫᄢቇ࡮ක‫ޔ‬㧞᧲੩ㇺஜᐽ㐳ኼක≮࠮ࡦ࠲࡯‫ޔ‬㧟࿖㓙ㄘᨋ᳓↥ᬺ⎇ⓥ࠮ࡦ࠲࡯‫ޔ‬㧠

⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯‫ޔ‬㧡ጤᚻකᄢ࡮ක㧕

− 70 −

‫ޣ‬⢛᥊‫⾰⊕Ⱞޤ‬ਛߩ . ဳߩࠕࠬࡄ࡜ࠡࡦᱷၮ .#UP ߅ࠃ߮ࠕࠬࡄ࡜ࠡࡦ㉄ᱷၮ .#UR ߪ‫ޔ‬⠧ൻ‫⚡ޔ‬ᄖ✢ߥߤߐ߹ߑ߹ߥࠬ࠻࡟ࠬೝỗߦ߅޿ߡ⇣ᕈൻ߹ߚߪ࡜࠮ࡒൻߐࠇߡ߅ߊߎߣ߇⍮ࠄࠇߡ޿ࠆ‫৻ޕ‬ᣇ‫↢ޔ‬૕ౝߢߪ‫ޔ‬D-Asp ߹ߚߪ L-isoAsp ࠍ L-Asp ߳ᚯߔ෻ᔕࠍ⸅ᇦߔࠆ㉂⚛ protein
L-isoasprtyl (D-aspertyl) methyltransferase (PIMT) ߇ሽ࿷ߒ‫ߩߎޔ‬㉂⚛ߩᰳ៊ࡑ࠙ࠬߪ‫⊒ࠎ߆ࠎߡޔ‬૞ߥߤ⣖ߦ㓚ኂ߇⹺߼ࠄࠇࠆ‫ߩߎޔࠄ߇ߥߒ߆ߒޕ‬㉂⚛ߪ⣖ߦᵴᕈ߇㜞޿߽ߩߩ‫ޔ‬૕ౝߦᐢߊಽ
Ꮣߒߡ޿ࠆ‫ߩߎߡߞ߇ߚߒޕ‬㉂⚛ᵴᕈߩૐਅߪ⣖એᄖߩ⤳ེߦ߅޿ߡ߽⇣ᕈ૕ൻ޽ࠆ޿ߪ࡜࠮ࡒ
ൻߒߚⰮ⊕⾰ߩ㓸Ⓧ޽ࠆ޿ߪ↢૕߳ߩᓇ㗀߇⠨߃ࠄࠇࠆ‫ޕ‬‫ޣ‬ᣇᴺ‫ޤ‬⢖⢩਄⊹↱᧪ߩ⚦⢩ᩣߢ޽ࠆ A549 ⚦⢩ߦ⊒⃻ߔࠆ PIMT ㆮવሶࠍ shRNA ࠍ↪޿ߡࡁ࠶ࠢ࠳࠙ࡦߒ‫ޔ‬਄⊹♽ߩࡑ࡯ࠞ࡯ߢ޽ࠆ E-cadoherin ߅ࠃ߮㑆⪲♽ߩࡑ࡯ࠞ࡯ߢ޽ࠆ Vimentin ߦߡ
Epithelial-to- mesencymal transitin (EMT) ࠍᬌ⸛ߒߚ‫ޔߚ߹ޕ‬᭴ㅧ⇣Ᏹࠍ⿠ߎߒߚⰮ⊕⾰ߦࠃࠆዊ⢩૕ࠬ࠻࡟ࠬߩ᦭ήࠍᬌ⸛ߒߚ‫ޕ‬PIMT ㆮવሶࠍࡁ࠶ࠢ࠳࠙ࡦߔࠆߎߣߢ⹺߼ࠄࠇߚⰮ⊕⾰⊒⃻ߩ㆑޿ࠍ㧞ᰴర㔚᳇ᵒേߢᬌ⸛ߒߚ‫ޔߚ߹ޕ‬Paenidase I ࠍ⚵ߺวࠊߖࠆߎߣߦࠃࠅ D-Asp ߩ᦭ήߦߟ
޿ߡ߽ᬌ⸛ߒߚ‫ߩ⾰⊕Ⱞޕ‬หቯߪ㘧ⴕᤨ㑆ဳ⾰㊂ಽᨆߦߡⴕߞߚ‫ޕ‬‫⚿ޣ‬ᨐ߅ࠃ߮⠨ኤ‫ޤ‬A549 ⚦⢩ߦ PIMT-shRNA ࠍ⊒⃻ߐߖࠆߣ PIMT ㆮવሶߪ࠳࠙ࡦ࡟ࠡࡘ࡟࡯
࡚ࠪࡦߒ‫⚦ߦࠄߐޔ‬⢩ߩᒻᘒ߇ᄌൻߒߡ‫⚦⧘⛽❫ޔ‬⢩᭽ߩ⚜㍝ᒻߩᒻࠍ␜ߒߚ‫ޕ‬਄⊹♽ࡑ࡯ࠞ࡯ߩ E-cadherin ߪૐਅߒ‫৻ޔ‬ᣇ㑆⪲♽ߩࡑ࡯ࠞ࡯ߢ޽ࠆ Vimentin ߇⊒⃻ߒߡ߈ߚ‫ޕ‬ዊ⢩૕ࠬ࠻࡟ࠬ
ࡑ࡯ࠞ࡯ߩ⊒⃻ߢߪ GRP78 ߇᦭ᗧߦ਄᣹ߒ‫ޔ‬ዊ⢩૕ࠬ࠻࡟ࠬ߇⿠ߎߞߡ޿ࠆߎߣ߇⏕⹺ߐࠇߚ‫ޕ‬Cont-shRNA ⊒⃻⚦⢩ߣ PIMT-shRNA ⊒⃻⚦⢩ߩ᛽಴ᶧࠍ㧞ᰴర㔚᳇ᵒേߢಽᨆߒ‫ޔ‬ᄌൻࠍ␜ߔⰮ
⊕⾰ࠍ⾰㊂ಽᨆߢหቯߔࠆߣ‫ޔ‬ᣂߚߦ࡟࠴ࡁࠗࡦ㉄วᚑߦ㑐ਈߔࠆ㉂⚛߅ࠃ߮ catenin ߩૐਅࠍ⹺߼ߚ‫ߩࠄࠇߎޕ‬ಽሶߪ⢖✢⛽∝ߩ⊒∝‫∛ޔ‬᳇ߩㅴⴕߦ㑐ਈߔࠆ࿃ሶߢ‫ޔ‬PIMT ߩ⊒⃻ૐਅ߇ዊ⢩૕ࠬ࠻࡟ࠬࠍ੺ߒߚ EMT ߦࠃࠆ✢⛽ൻߩේ࿃ߦߥࠅ߁ࠆߎߣ߇␜ໂߐࠇߚ‫ޕ‬⢖✢⛽∝ߢߪࡋ࡞ࡍࠬ࠙ࠗ࡞ࠬ⟲ߩᗵᨴ߇␜ໂߐࠇߡ߅ࠅ‫ޔ‬EBV ߦࠃࠆ PIMT ⊒⃻ࠍᬌ⸛ਛߢ޽ࠆ‫ޕ‬19) ቇળ⊒⴫㧦 ᐕᐲᣣᧄᯏળቇળ᧲ർᡰㇱ╙ 46 ᦼ⑺ቄ⻠Ṷળ⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫↰⑺ޔ‬ᄢቇ㧔⑺↰Ꮢ㧕Ṷ㗴ฬ㧦ੇᑼᓸ☳⎈ߒߚ᧖᧚ߩ㉂⚛♧ᄌ឵࡮ࠛ࠲ࡁ࡯࡞ᄌ឵ߩ⎇ⓥ⊒⴫⠪㧦᪢Ỉ ବ╷‫ޔ‬ዊᨋ ᷕ৻‫ޔ‬㜞ᯅ ᱞᒾ‫ޔ‬દ⮮ ৻ᔒ‫ޔ‬દ⮮ ᣂ㧔⑺↰⋵┙ᄢቇ㧕ㅴ⮮ ᣽⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ ᧁ⾰♽ࡃࠗࠝࠛ࠲ࡁ࡯࡞ࠍ⵾ㅧߔࠆ㓙㧘ᧁ᧚ߩ᭴ᚑⷐ⚛ߢ޽ࠆ࠮࡞ࡠ࡯ࠬ㧘ࡋࡒ࠮࡞ࡠ࡯ࠬ㧘࡝ࠣ࠾ࡦߩਛߢ㧘࠮࡞ࡠ࡯ࠬ߿ࡋࡒ࠮࡞ࡠ࡯ࠬࠍ᭴ᚑන♧ߦ߹ߢട᳓ಽ⸃㧔♧ൻ㧕ߒ㧘ᓧࠄࠇߚ♧ࠍ⊒㉂ߦࠃࠅࠛ࠲ࡁ࡯࡞ߦᄌ឵ߔࠆᔅⷐ߇޽ࠆ  㧚ߒ߆ߒߥ߇ࠄ㧘ᧁ᧚ࠍ↪޿ߚਗⴕⶄ⊒㉂ߩ
ᛛⴚߪ㧘♧ൻߩ㓙ߩᱷᷲ߇⊒㉂ࠍ㒖ኂߔࠆߚ߼⏕┙ߦ⥋ߞߡ޿ߥ޿㧚ᧄ⎇ⓥߢߪ‫ੇޔ‬ᑼᓸ☳⎈ߦࠃࠅ⚿᥏ൻᐲࠍૐਅߐߖߚᧁ᧚ᓸ☳ᧃ߆ࠄࡃࠗࠝࠛ࠲ࡁ࡯࡞ࠍ⵾ㅧߔࠆߚ߼㧘㉂⚛ߦࠃࠆ㜞Ớᐲ♧ൻߣ㉂Უߦࠃࠆࠛ࠲ࡁ࡯࡞⊒㉂ࠍᬌ⸛ߒߚ‫⊒࡞࡯ࡁ࠲ࠛޕ‬㉂⹜㛎ߪ㧘㉂⚛♧ൻߒᓧࠄࠇߚᧁ☳ᱷᷲࠍ฽߻♧ൻᶧࠍ㧘࿕ᶧಽ㔌ߒߚ߽ߩߣᧁ☳ᱷᷲࠍ฽ࠎߛ߹߹ߩ߽ߩߢታᣉߒߚ‫ߚ߹ޕ‬㧘♧ൻߣ⊒㉂ࠍหᤨߦⴕ߁ਗⴕⶄ⊒㉂ߦߟ޿ߡ߽หߓߊ⹜㛎ࠍⴕߞߚ㧚ߘߩ⚿ᨐ㧘ਗⴕⶄ⊒㉂ࠍⴕߞߚ
⹜㛎ߦ߅޿ߡ㧘⊒㉂ᤨ㑆ߩ⍴❗߇ⷰኤߢ߈㧘ߥ߅਌ߟࠛ࠲ࡁ࡯࡞ࠍ㜞෼₸ߢᓧࠆߎߣ߇ߢ߈ߚ‫ޕ‬− 71 −

20) ⊒⴫ቇળ㧦╙  ࿁ᣣᧄ↢‛Ꮏቇળᄢળ
⊒⴫ᣣߣ႐ᚲ㧦 ᐕ  ᦬  ᣣ‫࠻࠶ࡒࠨ࡯࠲ࡦ࠮ࡦ࡚ࠪࡦࡌࡦࠦ࠼࡞࡯ࡢޔ‬㧔ችፒ⋵ችፒᏒ㧕Ṷ㗴ฬ㧦ᷡ㈬ߩ๒๧ߦ㑐ଥߔࠆࠕࡒࡁ㉄ઍ⻢↥‛ߦ෸߷ߔࡈࠖ࠴ࡦ㉄ߩᓇ㗀⊒⴫⠪㧦٤㜞ᯅ ੳ㧔⑺↰✚㘩⎇㧕‫ޔ‬૒‫౏ᧁޘ‬ᄥ‫ޔ‬દ⮮ବᒾ‫ޔ‬ጤ㊁ำᄦ㧔⑺↰⋵ᄢ㧕‫┙⋵↰⑺ߢ߹ࠇߎޤ⊛⋡ޣ‬ᄢቇߢߪ‫ޔ‬ᷡ㈬ߩ๒๧ߦ㑐ଥߔࠆൻว‛ࠍត⚝ߒ‫ޔ‬i-ࡉ࠲ࡁ࡯࡞‫ޔ‬i-ࠕࡒ࡞ࠕ࡞ࠦ࡯࡞‫ޔ࡞࡯࠰ࡠ࠴ޔ‬Ǫ-ࡈࠚ࠾࡞ࠛ࠲ࡁ࡯࡞‫߇㉄ࠢࡂࠦޔ࡞࡯ࡎ࠻ࡊ࡝࠻ޔ‬㑐ଥߒ‫ߎޔ‬ࠇࠄߩൻว‛ߪᷡ㈬ߩ߽ࠈߺೋᦼߩ㉂ᲣჇᱺߦ߅ߌࠆࠕࡒࡁ㉄ઍ⻢↥‛ߢ޽ࠆߎߣ‫ߺࠈ߽ޔ‬ೋᦼߩࠕࡒࡁ㉄Ớᐲ߇ᄢ߈ߊᓇ㗀ߔࠆߎߣࠍ᣿ࠄ߆ߦߒߚ‫ߺࠈ߽ޕ‬ೋᦼߦ߅ߌࠆࠕࡒࡁ㉄Ớᐲߪ㤕߆ࠄᜬㄟ߹ࠇࠆ㊂ߣ⫳☨࠲ࡦࡄࠢ⾰߇㤕㉂⚛ߦࠃࠅಽ⸃ߐࠇߡ↢ᚑߔࠆ㊂ߩว⸘ߢ޽ࠆ߇‫ޔ‬㤕ߦ↱᧪ߔࠆࠕࡒࡁ㉄㊂ߦߟ޿ߡ㤕ߩㆆ㔌ࠕࡒࡁ㉄㊂ߣࡈࠖ࠲࡯࠯ᵴᕈ߇ᱜߩ⋧㑐ߢ޽ࠆߎߣࠍ␜ߒߚ
㧝㧕‫⹜ᧄޕ‬㛎ߢߪ⫳☨࠲ࡦࡄࠢ⾰ߩ㉂⚛ಽ⸃ߦᓇ㗀ߔࠆߣႎ๔ߐࠇߡ޿ࠆࡈࠖ࠴ࡦ㉄ߩᓇ㗀ߦߟ޿ߡᬌ⸛ߒߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ ☨✚ޤ‬100㨓ߩ 3 Ბ઀ㄟߺߦࠃࠆ⹜㛎㉯ㅧࠍⴕߞߚ‫઀ޕ‬ㄟ඙ಽߪ‫ޔ‬ኻᾖ㧔No.1㧕‫ࡦ࠴ࠖࡈޔ‬㉄ᷝട㧔No.2)‫࠯࡯࠲ࠖࡈޔ‬ᷝട㧔No.3㧕ߩ 3 ඙ಽߣߒ‫ޔ‬ฦ඙ಽߏߣߦ㉢ᣣᢙ 5 ᣣ‫ޔ‬10 ᣣ‫ޔ‬15 ᣣ‫ޔ‬
20 ᣣ‫ޔ‬30 ᣣߩᚑಽࠍಽᨆߒߚ‫ࠍޠࡁࡑࠕޟ࠯࡯࠲ࠖࡈߪ࠯࡯࠲ࠖࡈޕ‬಴⊒᧚ᢱߣߒߡ SDS-PAGE㔚᳇ᵒേߢන৻ࡃࡦ࠼ࠍ␜ߔ߹ߢ♖⵾ߒߚ߽ߩࠍ૶↪ߒߚ‫ߪ㉄ࡦ࠴ࠖࡈޕ‬๺శ⚐⮎ߩࡈࠖ࠴ࡦ㉄
12Na Ⴎࠍ૶↪ߒߚ‫ޕ‬‫⚿ޣ‬ᨐ෸߮⠨ኤ‫㉄ࡦ࠴ࠖࡈޤ‬ᷝട઀ㄟߺߪ࠺ࡦࡊࡦಽ⸃߇ଦㅴߐࠇ࠲ࡦࡄࠢ⾰ಽ⸃߇ᛥ߃ࠄࠇࠆ
ߎߣ߇⏕⹺ߐࠇߚ‫ߩߎޕ‬ℂ↱ߪࡈࠖ࠴ࡦ㉄ᷝടߦࠃࠅ -ࠕࡒ࡜࡯࠯ߩήലๆ⌕߇⸃ᶖߐࠇ‫࡞ࠣޔ‬࠹࡝ࡦߩਇṁൻ߇↢ߓߚߚ߼ߣផ⺰ߐࠇࠆ‫ߦࠄߐޕ‬㉢ೋᦼߩ⊒㉂߇ᕆㅴߒࠕࡒࡁ㉄⾗ൻ㊂߇ᄙߊ‫ޔ‬ߘߩઍ⻢↥‛ߢ޽ࠆ i-ࡉ࠲ࡁ࡯࡞‫ޔ‬i-ࠕࡒ࡞ࠕ࡞ࠦ࡯࡞‫߇㉄ࠢࡂࠦޔ‬Ⴧടߒߚ‫࠯࡯࠲ࠖࡈޕ‬ᷝട઀ㄟߪ࠺ࡦࡊࡦಽ⸃ߪኻᾖߣ߶߷หߓߢ޽ࠅ‫↢࡞࡯ࠦ࡞ࠕ⚐ޔ‬ᚑ㊂߽ኻᾖߣหߓߢ޽ࠆߎߣ߆ࠄਗⴕⶄ⊒㉂ߦߪᓇ㗀߇ߥ޿߽ߩߣᕁࠊࠇࠆ‫৻ޕ‬ᣇ‫ޔ‬i-ࡉ࠲ࡁ࡯࡞‫ޔ‬i-ࠕࡒ࡞ࠕ࡞ࠦ࡯࡞‫ߪ㉄ࠢࡂࠦޔ‬ኻᾖߣห⒟ᐲߢ޽ࠅߥ߇ࠄ⵾ᚑ㈬ߩࠕࡒࡁ㉄Ớᐲ߇ᄙ޿ߎߣ߆ࠄ࠲ࡦࡄࠢ⾰ߩಽ⸃ߪଦㅴߐࠇߚ
߽ߩߣ್ᢿߐࠇߚ‫ޕ‬‫ޣ‬ෳ⠨ᢥ₂‫ޤ‬㧝㧕૒‫౏ᧁޘ‬ᄥ,દ⮮ବᒾ,ਛỈિ㊀,ጤ㊁ำᄦ(⑺↰⋵ᄢ),㜞ᯅੳ(⑺↰✚㘩⎇),ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸,61,87 (2009)21) ⊒⴫ቇળ㧦╙㧤࿁♻⁁⩶ಽሶ↢‛ቇࠦࡦࡈࠔ࡟ࡦࠬ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 11 ᦬ 18 ᣣ‫ޔ‬ᐢፉᄢቇ㧔᧲ᐢፉᏒ㧕Ṷ㗴ฬ㧦ࠬ࠻࡟ࠬᔕ╵ mRNA ᄌേ⸃ᨆߦࠃࠆ㤕⩶㧔Aspergillus oryzae㧕ߩᯏ⢻⊛ᄖ᧪ᕈㆮવሶត⚝⊒⴫⠪㧦ዊ═ේඳା㧝‫ޔ‬㜞ᯅ⍾❱㧝‫ޔ‬੖๧ൎ਽㧞
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞᧲ർᄢ㒮ㄘ࡮↢‛↥ᬺഃᚑ㧕‫ޤ⊛⋡ޣ‬㤕⩶ߩᵴᕈဳ DNA ࠻࡜ࡦࠬࡐ࠱ࡦ Crawler ߪ‫ޔ‬ಽ↢⢩ሶߦ߅ߌࠆ㜞Ớᐲߩ Cu2+߿㜞᷷ߥߤߩࠬ࠻࡟ࠬಣℂߦࠃߞߡォ⒖ᵴᕈࠍ⊒⃻ߔࠆ‫ޔ߈ߣߩߎޕ‬Crawler-mRNA ߪࠬࡊ࡜ࠗࠪࡦࠣ߿
poly(A)ઃടࠍฃߌߚਇቢోߥಽሶ⒳߇ᷫዋߒࠗࡦ࠲ࠢ࠻ߥో㐳 mRNA Ყ₸߇Ⴧടߔࠆߎߣ߇⹺

− 72 −

߼ࠄࠇ‫ޔ‬Crawler ߩᯏ⢻ൻ㧔ォ⒖ᵴᕈൻ㧕ߦߟߥ߇ࠆߎߣࠍ᣿ࠄ߆ߦߒߡ߈ߚ㧝㧕‫⎇ᧄޔߢߎߘޕ‬ⓥߢߪࠥࡁࡓߩᄙ᭽ൻᯏ᭴ߩ⸃᣿ࠍ⋡⊛ߦ࠻࡜ࡦࠬࡐ࠱ࡦ Crawler ࠍࠬ࠻࡟ࠬ᧦ઙਅߢᵴᕈൻߔࠆ
ᄖ᧪ᕈㆮવሶߩࡕ࠺࡞ߣߒ‫ ోޔ‬mRNA ߩࠬࡊ࡜ࠗࠪࡦࠣᄌേࠍᲧセ⸃ᨆߔࠆߎߣߦࠃࠅ‫ޔ‬ᣂߚߦᯏ⢻ൻߔࠆᄖ᧪ᕈㆮવሶߩត⚝ࠍⴕߞߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ޤ‬Cu2+ࠬ࠻࡟ࠬಣℂᓟ߅ࠃ߮ኻᾖ඙ߩಽ↢⢩ሶࠃࠅ total RNA ࠍ᛽಴ߒ‫ ోޔ‬cDNA ࠪ࡯ࠤࡦࠪࡦࠣࠍၮߦ DOGAN ߩࠕࡁ࠹࡯࡚ࠪࡦ࠺࡯࠲߽ട߃ߚࠬ࠻࡟ࠬᔕ╵ cDNA ࡉ࡜࠙ࠩ࡯ࠍ᭴▽ߒߚ‫޿ߥ޿ߡࠇߐߥ߇ࡦ࡚ࠪ࡯࠹ࡁࠕޕ‬㗔ၞࠍਛᔃߦ‫ޔ‬Crawler-mRNA ߣห᭽ߦࠬ࠻࡟ࠬᔕ╵ߦࠃࠅ‫ޔ‬ᒝ޿ࠬࡊ࡜ࠗࠪࡦࠣ㒖ኂ߇⹺߼ࠄࠇࠆ DNA ㈩೉ࠍ᛽಴ߒ‫ޔ‬Blast ᬌ⚝ߦࠃࠆ⸃ᨆࠍⴕߞߚ‫ޕ‬‫⚿ޣ‬ᨐ‫ޤ‬Chromosome1 ߩ SC009 ࠃࠅ A. niger ߩ DNA ࠻࡜ࡦࠬࡐ࠱ࡦ Tan1 ߦ⋧หᕈߩ㜞޿㈩೉߿
࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦߩ gag ᭽㈩೉ߥߤ߇⷗಴ߐࠇߚ‫ޕ‬Tan1 ᭽㈩೉ߪ 558 ࠕࡒࡁ㉄߆ࠄߥࠆtransposase ࠍࠦ࡯࠼ߒ‫ޔ‬42bp ߩቢోߥ TIR ߣ 2bp㧔TA㧕ߩ TSD ㈩೉ߦ࿐߹ࠇߚᣂⷙߩ DNA ࠻࡜ࡦࠬࡐ࠱ࡦߢ޽ߞߚ‫ޕ‬A. oryzae ࠥࡁࡓౝࠍᬌ⚝ߒߚߣߎࠈ‫ޔ‬7 ࠦࡇ࡯ߩቢోဳ‫ޔ‬4 ࠦࡇ࡯ߩᄌ⇣ဳ߅ࠃ߮⚂ 90 ࠦࡇ࡯ߩ degenerate ဳ߇ಽᏓߒ‫ޔ‬ᄙࠦࡇ࡯ߢ޽ࠆߎߣ߆ࠄ Crawler ห᭽ߦォ⒖ᵴᕈൻߩน⢻ᕈ߽␜ߐࠇߚ‫ޕ‬1)ㄘൻᄢળ⻠Ṷⷐᣦ㓸‫ޔ‬p284㧔2009㧕22) ⊒⴫ቇળ㧦╙ 83 ࿁ᣣᧄ↢ൻቇળ࡮╙ 33 ࿁ᣣᧄಽሶ↢‛ቇળวหᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 12 ᦬ 7 ᣣ‫␹ޔ‬ᚭࡐ࡯࠻ࠕࠗ࡜ࡦ࠼㧔␹ᚭᏒ㧕Ṷ㗴ฬ㧦࡟࠾ࡦ㒖ኂࠨࡐ࠾ࡦ㧦᭴ㅧ࡮ᯏ⢻⋧㑐⸃ᨆ⊒⴫⠪㧦㜞ᯅ⍾❱‫ޔ‬ၳ ৻ਯ‫ޔ‬ᓟ⮮ ⁴*‫᧖ޔ‬ጊବඳ**(⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯‫↰⑺*ޔ‬ᄢቇ࡮Ꮏቇ⾗Ḯ‫↰⑺**ޔ‬ᄢቇ࡮කቇㇱ)‫ޔߪࡦ࠾࡟ޤ⊛⋡ޣ‬ਥߦ⣢⤳ਛߢ↢วᚑߐࠇ᭽‫ߥޘ‬ೝỗߢⴊਛߦ᡼಴ߐࠇⴊ࿶⺞▵ࠍⴕߞߡ޿ࠆ‫ޕ‬ࡅ࠻ဳ࡟࠾ࡦߪ⚂ 20 ᐕ೨ߦߘߩ⚵឵߃૕ߩ૞ᚑߦᚑഞߒ‫ޔ‬㨄✢⚿᥏᭴ㅧ⸃ᨆ߽ㅴࠎߢ߅ࠅ‫ࠇߘޔ‬ࠍၮߦ᛫࡟࠾ࡦ⵾೷ߩ㐿⊒߽ⴕࠊࠇߡ޿ࠆ‫৻ޕ‬ᣇ‫ⴊޔ‬࿶ᛥ೙ࠍᮡ᭙ߒߚ․ቯ଻ஜ↪㘩ຠߩ㐿⊒߽⋓ࠎߦⴕࠊࠇߡ޿ࠆ‫ࡦࠪࡦ࠹ࠝࠡࡦࠕޔߪࠄࠇߘޕ‬ᄌ឵㉂⚛ࠍᮡ⊛ߣߒߚ߽ߩߢ‫ࠍࡦ࠾࡟ޔ‬ᮡ⊛ߣߒߚ㘩‛↱᧪ᚑಽ⎇ⓥߪᱴߤⴕࠊࠇߡ޿ߥ޿‫ᦨޕ‬ㄭ‫ޔ‬ᚒ‫ࡦ࠾࡟ߦ࠭ࠗ࠳ߪޘ‬㒖ኂᵴᕈࠍ⷗಴ߒ‫ޔ‬ߘߩ᭴ㅧࠍ࠰ࡗࠨࡐ࠾ࡦ I ߣหቯߒߚ‫⎇ᧄޕ‬ⓥߢߪ‫ޔ‬ฦ⒳ࠨࡐ࠾ࡦ㘃ߦࠃࠆ࡟࠾ࡦ㒖ኂ⢻ߦߟ޿ߡᬌ⸛ߒ‫ࡦ࠾࡟ޔ‬㒖ኂߦᔅ㗇ߥࠨࡐ࠾ࡦߩ᭴ㅧࠍ᣿ࠄ߆ߣߒߚߩߢႎ๔ߔࠆ‫ޕ‬‫ޣ‬ᣇᴺ‫ޤ‬㒖ኂᬌቯ↪ߩ⚵឵߃ဳࡅ࠻࡟࠾ࡦߪ‫࡮ࠬ࡞ࠗ࠙ࡠࡘࠠࡃޔ‬᣸⯻⚦⢩♽ࠍ↪޿ߡ⊒⃻ߒ‫ޔ‬♖ ⵾ ߒ ߚ ᮡ ຠ ࠍ ↪ ޿ ߚ ‫ ࡦ ࠾ ࡟ ޕ‬ᵴ ᕈ ߪ ‫ ޔ‬ᣂ ߚ ߦ 㐿 ⊒ ߒ ߚ Ⱟ శ ᶖ శ ၮ ⾰ (Nma-Ile-HisPro-Phe-His-Leu*Val-Ile-Thr-His-Lys(Dnp)-D-Arg-D-Agr-NH2㧕ࠍ↪޿ߡ᷹ቯߒߚ‫ޕ‬‫⚿ޣ‬ᨐߣ⠨ኤ‫ࡦ࠾ࡐࠨޤ‬㘃෸߮ኻᔕߔࠆࠨࡐࠥࡁ࡯࡞㘃චᢙ⒳㘃ࠍ↪޿ߡ࡟࠾ࡦ㒖ኂᵴᕈࠍᬌ⸛
ߒߚ‫⚿ߩߘޕ‬ᨐ‫ ࡦ࠾ࡐࠨࡗ࠰ޔ‬II ߅ࠃ߮ࡎ࠙ࠠࠡ↱᧪ࠨࡐ࠾ࡦ㘃߇࠰ࡗࠨࡐ࠾ࡦ I ߣห⒟ᐲߩ㒖ኂࠍ␜ߒߚ‫ ߿ࡦ࠴࡝࡞࠴࡝ࠣޔࡦ࠾ࡐࠨ࠷࠮ࠢ࠴ޔߚ߹ޕ‬MGGA ߦ߽࡟࠾ࡦ㒖ኂᵴᕈ߇⹺߼ࠄࠇ
ߚ‫ࠄࠇߎޔࠄ߇ߥߒ߆ߒޕ‬એᄖߩࠨࡐ࠾ࡦ㘃߿ࠨࡐࠥࡁ࡯࡞㘃ߦߪ࡟࠾ࡦ㒖ኂᵴᕈߪ⹺߼ࠄࠇߥ
߆ߞߚ‫ޕ‬㒖ኂࠍ᦭ߔࠆࠨࡐ࠾ࡦ㘃ߪ‫ޔ‬㧟૏ߦࠣ࡞ࠢࡠࡦ㉄߇⚿วߒߡ޿ࠆࠣ࡞ࠢࡠ࠾࠼ࠨࡐ࠾ࡦ
ߢ޽ࠆߎߣ߇␜ߐࠇߚ‫ޕ‬

− 73 −23) ⊒⴫ቇળ㧦╙ 83 ࿁ᣣᧄ↢ൻቇળ࡮╙ 33 ࿁ᣣᧄಽሶ↢‛ቇળวหᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 12 ᦬ 7 ᣣ‫␹ޔ‬ᚭࡐ࡯࠻ࠕࠗ࡜ࡦ࠼㧔␹ᚭᏒ㧕Ṷ㗴ฬ㧦ේᩭᓸ↢‛↱᧪ D -ࠕࠬࡄ࡜ࠡࡦ㉄ࠛࡦ࠼ࡍࡊ࠴࠳࡯࠯㧔Paenidase㧕ㆮવሶߩࠥࡁࡓ࡜ࠗࡉ࡜࡝࡯߆ࠄߩන㔌⊒⴫⠪㧦㖴Ỉ ᖗ㧔⁛ᴺ࿖㓙ㄘᨋ᳓↥ᬺ⎇ⓥ࠮㧕‫ޔ‬㜞ᯅ⍾❱㧔⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮㧕           
Paenidase ߪේᩭᓸ↢‛߆ࠄೋ߼ߡන㔌ߐࠇߚ D ဳࠕࠬࡄ࡜ࠡࡦ㉄ߦ․⇣⊛ߥࠛࡦ࠼ࡍࡊ࠴࠳࡯
࠯ߢ޽ࠆ (S. Takahashi et al., J. Biochem. 139,197, 2006)‫੹ޕ‬࿁ᚒ‫ޔߪޘ‬PCR ᴺߦࠃࠅขᓧߒߚ
paenidase ㆮવሶߩႮၮ㈩೉ࠍౣ⏕⹺ߔࠆߚ߼ߦ‫ࠅࠃ࡯࡝࡜ࡉࠗ࡜ࡓࡁࠥޔ‬ㆮવሶࠍන㔌ߒ‫ߩߘޔ‬㈩೉ࠍ⺞ߴߚ‫ޕ‬Paenibacillus sp. B38 ᩣࠃࠅන㔌ߒࠥࡁࡓ DNA ࠍ↪޿ߡ࡚ࠪ࠶࠻ࠟࡦ࡜ࠗࡉ࡜࡝
࡯ࠍ૞ᚑߒ‫࡯࠾ࡠࠦޔ‬PCR ᴺߦࠃࠅ⋡⊛㈩೉ࠍ฽߻ࠢࡠ࡯ࡦࠍࠬࠢ࡝࡯࠾ࡦࠣߒߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬3,804bp ߩࠗࡦࠨ࡯࠻ࠍᜬߟࡊ࡜ࠬࡒ࠼㧔pUC118/K19㧕ࠍන㔌ߒߚ‫ޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ߩႮၮ㈩೉ࠍಽᨆߒ‫ޔ‬PCR ᴺߦࠃࠅᓧࠄࠇߚႮၮ㈩೉ߣᲧセߒߚߣߎࠈ‫ޔ‬⠡⸶㗔ၞߢߪႮၮߩ㆑޿ߪߥ
߆ߞߚ߇‫ޔ‬5’஥ߩ㕖⠡⸶㗔ၞߢ㈩೉߇⚵ߺ឵ࠊߞߡ޿ߚ‫ޔߢߎߘޕ‬ౣᐲ 5’஥ߩ㕖⠡⸶㗔ၞࠍ฽߻ࠢࡠ࡯ࡦࠍࠬࠢ࡝࡯࠾ࡦࠣߒߚߣߎࠈ‫ޔ‬4,071bp ߩࠗࡦࠨ࡯࠻ࠍᜬߟࡊ࡜ࠬࡒ࠼㧔pUC118/64㧕ࠍන㔌ߒߚ‫ޕ‬ᓧࠄࠇߚࡊ࡜ࠬࡒ࠼ߩႮၮ㈩೉ࠍಽᨆߒ‫ޔ‬pUC118/K19 ߩႮၮ㈩೉ߣ⍦⋫߇ߥ޿ߎߣࠍ
⏕⹺ߒߚ‫੹ޕ‬࿁⸃ᨆߒߚ㗔ၞߦߪ paenidase ㆮવሶࠍ฽߼ߡ᭴ㅧㆮવሶ߇ 4 ⒳㘃ሽ࿷ߔࠆߣផቯߐ
ࠇߚ‫ޕ‬Paenidase એᄖߩㆮવሶߪ‫ ࠇߙࠇߘޔ‬Clostridium cellulolyticum ↱᧪ Cellulosome anchoring
protein (30%)‫ޔ‬Brevibacillus brevis ↱᧪ ATPase ᭽࠲ࡦࡄࠢ⾰ (73%)‫ޔ‬ห↱᧪ 4-aminobutyrateaminotransferase ᭽࠲ࡦࡄࠢ⾰ (48%)ߣ⋧หᕈ߇޽ߞߚ‫ޕ‬24) ⊒⴫ቇળ㧦╙ 83 ࿁ᣣᧄ↢ൻቇળ࡮╙ 33 ࿁ᣣᧄಽሶ↢‛ቇળวหᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2010 ᐕ 12 ᦬ 7 ᣣ‫␹ޔ‬ᚭࡐ࡯࠻ࠕࠗ࡜ࡦ࠼㧔␹ᚭᏒ㧕Ṷ㗴ฬ㧦Protein isoasparatyl methyltransferase gene knock down induced human alveolar epithelial tomesenchymal cell transition through endoplasmic reticulum stress⊒⴫⠪㧦ዊ═ේ ᱜੱ 1㧘ᚭ↰ ᐕ✚ 2㧘㖴Ỉ ᖗ 3㧘೨ጊ ৻㓉 1㧘㜞ᯅ ⍾❱ 4㧘ጊౝ ᐢᐔ 5
㧝3ᗲᇫᄢ࡮㒮කቇ♽⎇ⓥ⑼࡮⮎ℂቇ㧘2 ᧲੩ㇺ⠧ੱ✚ว⎇ⓥᚲ㧘࿖㓙ㄘᨋ᳓↥⎇ⓥ࠮㧘4 ⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮㧘5 ጤᚻක⑼ᄢቇ࡮ౝ⑼Background: A mechanism of epithelial-mesenchymal cell transition (EMT) is potently involved in lungfibrosis. However, pathological findings from lung fibrosis in animal model were not completely
compatible with pathological findings from IPF patients. Proteins in the cells were modified withisomerization of L-asparatic acid under aging, UV stress, oxidative stress, chemical compounds. These
proteins undergo strutual change resulting in accumulatin of nonfunctional proteins in endoplasmic
reticulum(ER). Some of these proteins, however, are recoverd by protein isoasparatyl methyltransferase(PCMT1) to maintain protein structure and functions.Methods: For elucidation of the function of PCMT1
enzyme, A549 cells were transfected to inhibit PCMT1 gene expression utilizing PCMT1 shRNA vector.
EMT was evaluated by morphological and biochemical analysis with investigating expression of Vimentin,
E-cadherin, GRP78, GRP94, and HSC70. Differential proteomic analysis was performed by 2 dimentional− 74 −

eletcrophoresis follwed by analysis and identification with TOF-MS or MS/MS analysis.Results: A549
cells with PCMT1 knockdown indicates EMT evaluated by upregulation of vimentin and down regulationof E-cadherin,. Cells that had undergone EMT showed enhanced expression of ER stress marker protein
GRP78. Furthermore, proteomic analysis identified downregulated proteins including GRP94 and HSC70
and ALDH1A1 that is important enzyme for synthesizing retinoic acid.Conclusion: Our study showed thatknockdown of PCMT1 expression induced A549 alveolar epithelial cells to undergo EMT through ER
stress. Our data represent the novel mechanism of EMT in lung epithelial cells.

25) ቇળ⊒⴫㧦ᣣᧄࠛࡀ࡞ࠡ࡯ቇળ ╙㧢࿁ࡃࠗࠝࡑࠬ⑼ቇળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 1 ᦬ 13 ᣣ‫ޔ‬ᄢ㒋ᄢቇ㧔็↰Ꮢ㧕Ṷ㗴ฬ㧦࠮࡞ࡠ࡯ࠬ♽ࡃࠗࠝࡑࠬ߆ࠄߩ㧞Ბ㓏⊒㉂ᴺߦࠃࠆࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥⊒⴫⠪㧦ㅴ⮮ ᣽‫↰⷏ޔ‬ቁિ (⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯)‫ࠄ߆ࠬࡑࠝࠗࡃ♽ࠬ࡯ࡠ࡞࠮ޤ⊛⋡ޣ‬ૐࠦࠬ࠻ߢࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥ࠍⴕ߁ߚ߼ߦ‫ޔ‬ല₸⊛ߥ
೨ಣℂᛛⴚߩ㐿⊒ߣࡍࡦ࠻࡯ࠬ࡮ࡋࠠ࠰࡯ࠬ߆ࠄߩ㜞෼₸ࠛ࠲ࡁ࡯࡞↢↥ᛛⴚߩ㐿⊒߇᳞߼ࠄࠇ
ߡ޿ࠆ‫੹ޕ‬࿁‫ࠕ࠾ࡕࡦࠕޔ‬ಣℂࡃࠗࠝࡑࠬ㉂⚛♧ൻᶧࠍ↪޿ߡᣂⷙߥ㧞Ბ㓏⊒㉂ࠪࠬ࠹ࡓߦࠃࠆ
ࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥ߦߟ޿ߡᬌ⸛ࠍⴕߞߚߩߢႎ๔ߔࠆ‫ޕ‬‫ޣ‬ᣇᴺ߅ࠃ߮⚿ᨐ‫ࠬ࡜ࠣࠕࡇࡀޔࠬ࡜ࠣ࠴࠶ࠗࠬޔࠬࠨࡦࠕ࡝ࠛޔߪࠬࡑࠝࠗࡃޤ‬෸߮ࠛ࠱ࡁࠠ
࠿ࡗ࠽ࠡࠍ↪޿ߚ‫ޕ‬೨ಣℂ࡮♧ൻߪ‫ޔ‬਄᧛ࠄ 1)ߩᣇᴺࠍ↪޿ߚ‫ࠬ࡯࠰ࠠࡋޔߪ↥↢࡞࡯ࡁ࠲ࠛޕ‬߆ࠄߩࠛ࠲ࡁ࡯࡞↢↥ࠍ Saccharomyces cerevisiae‫⥄ࠍ↥↢࡞࡯ࡁ࠲ࠛߩࠄ߆ࠬ࡯࠻ࡦࡍޔ‬ὼ⇇ࠃࠅಽ㔌ߒߚ Pichia stipits SS1-2 ࠍ↪޿ߚ 2 Ბ㓏⊒㉂ᴺߦࠃࠅⴕߞߚ 2)‫⚿ߩߘޕ‬ᨐ‫ޔ‬ฦ⒳ࡃࠗࠝࡑࠬ
♧ൻᶧࠍ㧞Ბ㓏⊒㉂ᴺߢࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥ࠍⴕࠊߖࠆߎߣߦࠃࠅ P.stipits SS1-2 ࠍන⁛ߢ↪
޿ߚ႐วࠃࠅ߽ࠛ࠲ࡁ࡯࡞෼₸߇ะ਄ߒ‫ޔ‬૗ࠇߩࡃࠗࠝࡑ߽ࠬ 90%એ਄ߣߥߞߚ‫ޕ‬26) ⊒⴫ቇળ㧦The 8th International Aspergillus Meeting(Asperfest8)

The 26th Fungal Genetics Conference (FGC26)⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 14 ᣣ‫ޔ‬16 ᣣ‫ޔ‬Asilomar Conference Center㧔Asilomar, Pacific Grove, CA, USA)Ṷ㗴ฬ㧦Exploratory survey for potential transposable elements in
Aspergillus oryzae by a stress-fluctuation cDNA browser.
⊒⴫⠪㧦Hironobu Ogasawara1, Saori Takahashi1, and Katsuya Gomi2
1

Akita Res. Inst. Food and Brewing,2

Graduate School of Agricultural Science, Tohoku University

An active DNA transposon Crawler isolated from the genome of industrially important fungus
Aspergillus oryzae transposes under extreme stress conditions [1]. The mRNA analysis of Crawler in theconidiospores revealed that cryptic splicing and premature polyadenylation of the mRNA occurred under
the normal culture condition.

The increasing in mature mRNA molecules was caused by stress treatment

of CuSO4 or heat shock, which could stimulate the transposition events allowing the full-length and active− 75 −

transposase to be produced.
 In this study, we carried out direct high-throughput paired-end RNA sequencing to construct a
stress-fluctuation cDNA browser with DOGAN-DB to survey exogenous or transposon-like genes such as
Crawler in A. oryzae.

With comparison of expression pattern under extreme stress condition (CuSO4) tothe normal condition, several novel transcripts with open reading frames were identified in intergenic
regions, where none of genes have been annotated in DOGAN-DB. Full length of DNA sequences
encoding transposable elements were frequently identified.

Among them, a novel transposable element

homologous to Tan1 from A. niger was identified and tentatively designated AoTan1 that shows multiple
characteristics of class II transposon.

The elements are present as multiple copies in the genome of the

RIB40 strain, suggesting that AoTan1 is also expected to show a transposition activity.
1)H. Ogasawara et al. Fungal Genet. Biol. , 46, 441-449 (2009)27) ⊒⴫ቇળ㧦ᣣᧄㄘ⧓ൻቇળ 2011 ᐕᐲᄢળ⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 26 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦mRNA ᄌേ⸃ᨆߦࠃࠆ㤕⩶㧔Aspergillus oryzae㧕ߩࠬ࠻࡟ࠬᔕ╵ㆮવሶߩត⚝⊒⴫⠪㧦ዊ═ේඳା㧝‫ޔ‬㜞ᯅ⍾❱㧝‫ޔ‬੖๧ൎ਽㧞
㧔㧝⑺↰⋵✚㘩⎇࠮‫ޔ‬㧞᧲ർᄢ㒮ㄘ࡮↢‛↥ᬺഃᚑ㧕‫ޤ⊛⋡ޣ‬㜞Ớᐲߩ Cu2+ ߿㜞᷷ߥߤߩࠬ࠻࡟ࠬࠍฃߌࠆߣ㤕⩶ߩᵴᕈဳ DNA ࠻࡜ࡦࠬࡐ࠱ࡦ
Crawler ߪ mRNA ߩ cryptic splicing ߿ ORF ౝ poly(A)ઃട߇ᷫዋߒ‫ోޔ‬㐳 mRNA Ყ₸߇Ⴧടߔࠆ
ߎߣߢォ⒖ᵴᕈ㧔ᯏ⢻ൻ㧕ࠍ␜ߔ‫⎇ᧄޔߢߎߘޕ‬ⓥߢߪォ⒖᧦ઙਅߦ߅ߌࠆో mRNA ߩ splicing
ᄌേࠍᲧセ⸃ᨆߔࠆߎߣߦࠃࠅ‫ߡߞࠃߦࠬ࡟࠻ࠬޔ‬ᣂߚߦᯏ⢻ൻߔࠆㆮવሶߩត⚝ࠍⴕߞߚ‫ޕ‬‫ޣ‬ᣇᴺ‫ޤ‬Cu2+ಣℂࠍⴕߞߚಽ↢⢩ሶਛߩో cDNA ㈩೉᳿ቯࠍⴕ޿‫ޔ‬DOGAN ߩ DB ߽ട߃ߚࠬ࠻
࡟ࠬᔕ╵ cDNA ࡉ࡜࠙ࠩ࡯ࠍ᭴▽ߒߚ‫ޕ‬Crawler-mRNA ߣห᭽ߦࠬ࠻࡟ࠬᔕ╵ߦࠃࠅ‫ޔ‬ᒝ޿ splicing㒖ኂ߇⹺߼ࠄࠇࠆ DNA ㈩೉ࠍ᛽಴ߒ‫ޔ‬Blast ᬌ⚝ߦࠃࠆ⸃ᨆࠍⴕߞߚ‫ޕ‬‫⚿ޣ‬ᨐ‫ࡦ࡚ࠪ࡯࠹ࡁࠕᧂޤ‬㗔ၞࠃࠅ A. niger ߩ DNA ࠻࡜ࡦࠬࡐ࠱ࡦ Tan1 ߦ⋧หᕈߩ㜞޿㈩೉߿
࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦߩ gag ᭽㈩೉ߥߤ߇⷗಴ߐࠇߚ‫ޕ‬Tan1 ᭽㈩೉ߪ 558 ࠕࡒࡁ㉄߆ࠄߥࠆtransposase ࠍࠦ࡯࠼ߒ‫ޔ‬42bp ߩቢోߥ TIR ߣ 2bp㧔TA㧕ߩ TSD ㈩೉ߦ࿐߹ࠇߚᣂⷙߩ DNA ࠻࡜ࡦࠬࡐ࠱ࡦߢ޽ߞߚ‫ޕ‬ቢోဳ߿ degenerate ဳ߇ࠥࡁࡓౝߦᄙࠦࡇ࡯ߢಽᏓߒ‫ޔ‬ォ⒖ᵴᕈൻߩน⢻ᕈ߽␜ߐࠇߚ‫ޕ‬28) ቇળ⊒⴫㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 26 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦࠮࡞࡜࡯࠯㜞⊒⃻ Pichia pastoris ࠍ↪޿ߚ㧯㧮㧼ߦࠃࠆ࠮࡞ࡠ࡯ࠬ߆ࠄߩ⋥ធࡃࠗࠝࠛ࠲
ࡁ࡯࡞↢↥⊒⴫⠪㧦ㅴ⮮ ᣽‫ ᧄ┻ޔ‬ᶈ‫ޔ‬Ⴧ↰␽ሶ‫ޔ‬㜞ᯅఝ᢯‫ޔ‬ਣጊ㆏ሶ 1‫ޔ‬੖ච፲࿻ᣣሶ 1‫ޔ‬㞯ፉᱜᶈ 1㧔⑺↰✚㘩⎇‫ޔ‬1 ᧲ᄢ㒮ㄘ↢⑼㧕
ߎࠇ߹ߢߦ㧘ᚒ‫ޔߪޘ‬Pichia pastoris ࠍ↪޿ߚㆮવሶ⊒⃻♽ߢ Phanerochaete chrysosporium ↱᧪− 76 −

ߩࠛࡦ࠼ࠣ࡞ࠞ࠽࡯࠯(Cel5A)෸߮ -ࠣ࡞ࠦࠪ࠳࡯࠯(BGL3Acat‫ޔ‬BGL1Bcat)ߩ⊒⃻ߦᚑഞߒߡ޿ࠆ‫ ߦߢ߹ࠇߎޕ‬BGL3Acat ⊒⃻㉂Უߢ 10%࠮ࡠࡆࠝ࡯ࠬ߆ࠄ 47 ᤨ㑆ߢ 42g/L ߩࠛ࠲ࡁ࡯࡞↢↥ߦᚑഞߒߡ޿ࠆ‫੹ޕ‬࿁‫ޔ‬Cel6A,Cel5A,BGL3Acat ෸߮ BGL1Bcat ⊒⃻㉂Უߦࠃࠆ -ࠣ࡞ࠞࡦ߆ࠄߩ㧯㧮㧼ߦࠃࠆࠛ࠲ࡁ࡯࡞↢↥ߦߟ޿ߡᬌ⸛ࠍⴕߞߚߩߢႎ๔ߔࠆ‫ޕ‬BGL3Acat ⊒⃻㉂Უߪ‫ޔ‬㉂Უߩᱫ
⚦⢩₸߇㜞ߊߥࠆߎߣ߇‫ޔ‬BGL1Bcat ⊒⃻㉂Უߢߪ㉂Უߩᱫ⚦⢩₸ࠍૐߊᛥ߃ࠆߎߣ߇ߢ߈ߚ‫ߘޕ‬ߎߢ‫ޔ‬BGL1Bcat ⊒⃻㉂Უࠍ↪޿ߚ CBP ߦࠃࠆ 10%࠮ࡠࡆࠝ࡯ࠬ߆ࠄߩࠛ࠲ࡁ࡯࡞↢↥ࠍⴕߞߚߣߎࠈ‫ޔ‬20 ᤨ㑆ߢ 90%ߩࠛ࠲ࡁ࡯࡞෼₸ࠍᓧߚ‫ޔߦࠄߐޕ‬5%-ࠣ࡞ࠞࡦ߆ࠄ BGL1Bcat ⊒⃻㉂Უߣ Cel5A ⊒⃻㉂Უࠍ↪޿ߡ CBP ߦࠃࠆࠛ࠲ࡁ࡯࡞↢↥ࠍⴕ޿‫ޔ‬24 ᤨ㑆ߢ 80%ߩࠛ࠲ࡁ࡯࡞෼₸ࠍ㆐ᚑߒߚ‫ޕ‬29) ቇળ⊒⴫㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 27 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦ࡔࠗ࡜࡯࠼෻ᔕ↢ᚑ‛ߦࠃࠆࠕ࡞ࠦ࡯࡞⊒㉂㒖ኂᯏ᭴ߩᬌ⸽
⊒⴫⠪㧦⷏↰ ቁિ1‫ޔ‬ᚭ᧻ ߐ߿߆1‫ޔ‬૒‫⟤ ᧁޘ‬Ꮧሶ1‫ޔ‬ਃᯅ ⑲৻2‫ޔ‬ᨋ ␞ሶ2‫ޔ‬ㅴ⮮ ᣽1㧔1⑺↰✚㘩⎇‫ޔ‬2ࡃࠗࠝࠛ࠲ࡁ࡯࡞⎇ⓥ⚵ว㧕ࡃࠗࠝࡑࠬࠍේ᧚ᢱߣߒߚࠛ࠲ࡁ࡯࡞ߩ↢ᚑᎿ⒟ߪ‫ޔ‬೨ಣℂ‫♧ޔ‬ൻ߅ࠃ߮⊒㉂ߩਃᲑ㓏ߦಽ㘃ߐࠇࠆ‫ޕ‬೨ಣℂᎿ⒟ߩਛߦߪᒝߊᾲಣℂࠍߔࠆ߽ߩ߇޽ࠅ‫ޔ‬ᾲಣℂᎿ⒟ߦ߅޿ߡ᭽‫↢߇⾰‛ߥޘ‬ᚑߐࠇࠆ‫↢ޕ‬ᚑߐࠇࠆ‛⾰ߣߒߡࡔࠗ࡜࡯࠼෻ᔕ↢ᚑ‛㧔ࡔ࡜ࡁࠗࠫࡦ㧕߇޽ࠆ‫ߦߢ߹ࠇߎޕ‬ᚒ‫ޘ‬ߪ‫߇ࡦࠫࠗࡁ࡜ࡔޔ‬Pichia stipitisߦࠃࠆࠠࠪࡠ࡯ࠬ߆ࠄߩࠕ࡞ࠦ࡯࡞↢ᚑࠍ㒖ኂߔࠆߎߣࠍႎ๔ߒ
ߚ‫੹ޕ‬࿁‫⊒࡞࡯ࠦ࡞ࠕޔ‬㉂ਛߩ㉂Უ⚦⢩ߦ↱᧪ߔࠆઍ⻢‛ࠍ✂⟜⊛ߦ⸃ᨆ㧔ࡔ࠲ࡏࡠ࡯ࡓ⸃ᨆ㧕ߒ‫⊒࡞࡯ࠦ࡞ࠕࠆࠃߦࡦࠫࠗࡁ࡜ࡔޔ‬㉂㒖ኂᯏ᭴ߩ⸃᣿ࠍ⹜ߺߚ‫ࠍࡦࠫࠗࡁ࡜ࡔ ޕ‬฽߻ၭ࿾ߢ⊒㉂ࠍ24ᤨ㑆ⴕߞߚP. stipitis SS39-1ᩣߩ⚦⢩߆ࠄઍ⻢‛ࠍ⺞⵾ߒ‫⸃ࡓ࡯ࡠࡏ࠲ࡔޔ‬ᨆߦଏਈߒߚ‫ޕ‬ኻᾖߣߒߡห᭽ߩ⸃ᨆࠍวᚑၭ࿾ߢ⊒㉂ߐߖߚ⚦⢩ߦ㑐ߒߡ߽ⴕߞߚ‫⸃ࡓ࡯ࡠࡏ࠲ࡔޕ‬ᨆߩ⚿ᨐ‫ޔ‬ࡔ࡜ࡁࠗࠫࡦࠍ฽߻ၭ࿾ߢߪࡍࡦ࠻࡯ࠬ࡝ࡦ㉄⚻〝ߩฦઍ⻢‛߇ኻᾖࠃࠅ߽ᄙߊ⫾Ⓧߒߡ޿ߚ‫ޕ‬ߎߩ⚿ᨐ߆ࠄ‫⊒ࠆࠃߦࡦࠫࠗࡁ࡜ࡔޔ‬㉂㒖ኂߩਥߥⷐ࿃ߪࡍࡦ࠻࡯ࠬ࡝ࡦ㉄⚻〝ߩᠣੂߢ޽ࠆߎ
ߣ߇␜ໂߐࠇߚ‫ޕ‬30) ቇળ⊒⴫㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 27 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦ࡃࠗࠝࡑࠬ♧ൻᶧߦ߅ߌࠆࠕ࡞ࠦ࡯࡞⊒㉂㒖ኂᯏ᭴ߩ⸃᣿
⊒⴫⠪㧦⷏↰ ቁિ1‫ޔ‬ᚭ᧻ ߐ߿߆1‫ޔ‬૒‫⟤ ᧁޘ‬Ꮧሶ1‫ޔ‬਄᧛ Პ2‫ޔ‬ㅴ⮮ ᣽1㧔1⑺↰✚㘩⎇‫ޔ‬2JXࠛ
ࡀ࡞ࠡ࡯ਛ⎇㧕ᚒ‫ߩ⑼ࡀࠗߪޘ‬ᄙᐕ⨲ߢ޽ࠆࠛ࡝ࠕࡦࠨࠬࠍේ᧚ᢱߣߒߡ⺞⵾ߒߚࡃࠗࠝࡑࠬ♧ൻᶧ߆ࠄല₸⊛ߦࡃࠗࠝࠛ࠲ࡁ࡯࡞ࠍ↢ᚑߔࠆᛛⴚߩ㐿⊒ࠍⴕߞߡ޿ࠆ‫ޔߦߢ߹ࠇߎޕ‬ᚒ‫♧ࠬࡑࠝࠗࡃߪޘ‬ൻᶧߦ߅޿ߡPichia stipitisߦࠃࠆࠠࠪࡠ࡯ࠬ߆ࠄߩࠕ࡞ࠦ࡯࡞⊒㉂߇㒖ኂߐࠇࠆߎߣࠍႎ๔ߒߡ޿
ࠆ1)‫੹ޕ‬࿁‫⊒࡞࡯ࠦ࡞ࠕޔ‬㉂ਛߩ㉂Უ⚦⢩ߦ↱᧪ߔࠆઍ⻢‛ࠍ✂⟜⊛ߦ⸃ᨆ㧔ࡔ࠲ࡏࡠ࡯ࡓ⸃ᨆ㧕ߒ‫♧ࠬࡑࠝࠗࡃޔ‬ൻᶧߢߩࠕ࡞ࠦ࡯࡞⊒㉂ߦኻߔࠆ㒖ኂ࿃ሶߩ⸃᣿ࠍ⹜ߺߚ‫ޕ‬− 77 − ࡃࠗࠝࡑࠬ♧ൻᶧߢߩ⊒㉂㐿ᆎ߆ࠄ 24 ᤨ㑆ᓟߦ P. stipitis SS39-1 ᩣߩ⚦⢩߆ࠄઍ⻢‛ࠍ⺞⵾ߒ‫ޔ‬ࡔ࠲ࡏࡠ࡯ࡓ⸃ᨆߦଏਈߒߚ‫ޕ‬ኻᾖߣߒߡห᭽ߩ⸃ᨆࠍวᚑၭ࿾ߢ⊒㉂ߐߖߚ⚦⢩ߦ㑐ߒߡ߽ⴕߞߚ‫⸃ࡓ࡯ࡠࡏ࠲ࡔޕ‬ᨆߩ⚿ᨐ‫♧ࠬࡑࠝࠗࡃޔ‬ൻᶧߢߪࡍࡦ࠻࡯ࠬ࡝ࡦ㉄⚻〝ߩฦઍ⻢‛߇ኻᾖࠃࠅ߽ᄙߊ⫾Ⓧߒߡ߅ࠅ‫ߩ࡞࡯ࡁ࠲ࠛߚ߹ޔ‬೨㚟‛⾰ߢ޽ࠆࡇ࡞ࡆࡦ㉄߇⚂ 3 ୚㊂⫾Ⓧߒߡ޿ߚ‫ޕ‬ߎߩ⚿ᨐ߆ࠄ‫♧ࠬࡑࠝࠗࡃޔ‬ൻᶧߦ߅ߌࠆ㒖ኂⷐ࿃ߪࡍࡦ࠻࡯ࠬ࡝ࡦ㉄⚻〝ߩᡬੂߣࡇ࡞ࡆࡦ㉄એ㒠ߩઍ⻢߇㒖ኂߐࠇߡ޿ࠆߚ߼ߢ޽ࠆߣ␜ໂߐࠇߚ‫ޕ‬31) ⊒⴫ቇળ㧦2011 ᐕᐲᣣᧄㄘ⧓ൻቇળᄢળ
⊒⴫ᣣߣ႐ᚲ㧦2011 ᐕ 3 ᦬ 27 ᣣ‫ޔ‬੩ㇺᅚሶᄢቇ㧔੩ㇺᏒ㧕Ṷ㗴ฬ㧦☨↱᧪࡟࠾ࡦ㒖ኂ‛⾰ߩหቯߣ᭴ㅧᯏ⢻⋧㑐⸃ᨆ⊒⴫⠪㧦㜞ᯅ⍾❱㧝‫ޔ‬Ᏹ⋚㊁ື↢㧞‫ ⇗ޔ‬ᕺม㧝‫ޔ‬ዊ₹㇚ሶ㧟‫⟤⧛଻ޔ‬૫㧝‫ޔ‬㋈ᧁᄹ✜㧝‫ޔ‬ศỈ⚿ሶ㧞‫ޔ‬ᓟ⮮ ⁴㧠
㧔㧝⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯࡮㘩ຠടᎿ⎇ⓥᚲ‫ޔ‬㧞⑺↰⋵┙ᄢቇ࡮↢‛⾗Ḯ⑼ቇㇱ‫ޔ‬㧟
⑺↰⋵ㄘᨋ᳓↥ᛛⴚ࠮ࡦ࠲࡯࡮ㄘᬺ⹜㛎႐‫ޔ‬㧠⑺↰ᄢቇᄢቇ㒮࡮Ꮏቇ⾗Ḯቇ⎇ⓥ⑼㧕
࡟࠾ࡦߪ‫ޔ‬㕖Ᏹߦ․⇣ᕈߩ㜞޿ࠕࠬࡄ࡞࠹࠶ࠢࡊࡠ࠹ࠕ࡯࠯ߢ‫♽ࡦࠪࡦ࠹ࠝࠡࡦࠕ࡮ࡦ࠾࡟ޔ‬(RAS)ߦࠃࠆⴊ࿶೙ᓮᯏ᭴ߦ߅ߌࠆᓞㅦ㉂⚛ߣߒߡⷐߩᓎഀࠍᜬߞߡ޿ࠆ‫ޕ‬RAS ೙ᓮߦ㑐ㅪߒߡ‫ޔ‬ߎࠇ߹ߢ㘩‛↱᧪ࠕࡦࠡࠝ࠹ࡦࠪࡦᄌ឵㉂⚛ߩ㒖ኂࡍࡊ࠴࠼߇ฦ⒳㘩᧚ࠃࠅᢙᄙߊ⷗಴ߐࠇߡ޿
ࠆ‫ޔࠄ߇ߥߒ߆ߒޕ‬㘩‛↱᧪࡟࠾ࡦ㒖ኂ‛⾰ߦ㑐ߒߡߪ㉂⚛౉ᚻ߿ᵴᕈ᷹ቯߥߤߩ໧㗴߇޽ࠅ‫ޔ‬ᱴߤ⎇ⓥ߇ㅴࠎߢ޿ߥ߆ߞߚ‫ޕ‬ᚒ‫ᦨߪޘ‬ㄭᄢ⼺ߦ࡟࠾ࡦ㒖ኂᵴᕈࠍ⷗಴ߒ‫ߩߘޔ‬᭴ㅧࠍ࠰ࡗࠨࡐ
࠾ࡦ㧵ߣหቯߒߚߣߣ߽ߦࠨࡐ࠾ࡦߩ᭴ㅧߣᯏ⢻ߦߟ޿ߡᬌ⸛ߒߚ[1, 2]‫⎇ᧄޕ‬ⓥߢߪ‫ޔ‬ᄢ⼺એᄖߩ㘩᧚ࠃࠅ࡟࠾ࡦ㒖ኂ‛⾰ࠍត⚝ߒߚ⚿ᨐ‫ߦ☨ޔ‬ೋ߼ߡ࡟࠾ࡦ㒖ኂ‛⾰ࠍ⷗಴ߒߚ‫ޕ‬ᓧࠄࠇߚ㒖ኂ‛⾰ߪ‫ޔ‬ㆆ㔌ߩਇ㘻๺⢽⢌㉄ߢ޽ࠆࠝ࡟ࠗࡦ㉄ߣ࡝ࡁ࡯࡞㉄ߩᷙว‛ߢ޽ߞߚ‫ࠇߎޔߢߎߘޕ‬ࠄਇ㘻๺⢽⢌㉄߇න⁛ߢ߽࡟࠾ࡦ㒖ኂᵴᕈࠍᜬߟ߆ุ߆ࠍ‫ޔ‬Ꮢ⽼ߩ㜞⚐ᐲ⹜⮎ࠍ↪޿ߡᬌ⸛ߒߚ‫ޕ‬ߘߩ⚿ᨐ‫ޔ‬Ꮢ⽼ࠝ࡟ࠗࡦ㉄߿࡝ࡁ࡯࡞㉄ߢ߽ᒝ޿࡟࠾ࡦ㒖ኂᵴᕈࠍᜬߟߎߣ߇␜ߐࠇߚ‫ޔߚ߹ޕ‬࡝ࡁ࡯࡞㉄߿ࠝ࡟ࠗࡦ㉄એᄖߩ㜞ᐲਇ㘻๺⢽⢌㉄ࠍ↪޿ߡ࡟࠾ࡦ㒖ኂࠍᬌ⸛ߒߚߣߎࠈ‫ߚ޿↪ޔ‬ోߡߩਇ㘻๺⢽⢌㉄ߦ࡟࠾ࡦ㒖ኂᵴᕈ߇⹺߼ࠄࠇߚ‫ޔࠄ߇ߥߒ߆ߒޕ‬㘻๺⢽⢌㉄߿࠻࡝ࠣ࡝࠮࡝
࠼ߦ࡟࠾ࡦ㒖ኂᵴᕈߪ⹺߼ࠄࠇߥ߆ߞߚ‫ޕ‬એ਄ߩ⚿ᨐ߆ࠄ‫ޔ‬ㆆ㔌ਇ㘻๺⢽⢌㉄߇࡟࠾ࡦ㒖ኂᵴᕈࠍᜬߟߎߣ߇᣿ࠄ߆ߣߥߞߚ‫ޕ‬
1. S. Takahashi et al. Biosci. Biotechnol. Biochem., 72, 3232-3236 (2008)
2. S. Takahashi et al. Biomed. Res., 31, 155-159 (2010)− 78 −

㧢㧚ᄖㇱ⊒⴫⺰ᢥ᭎ⷐ (16 ઙ)1) ⺰ᢥ㗴ฬ㧦 Inhibition of human renin activity by saponins.
⪺⠪ฬ㧦 Saori Takahashi, Kazuyuki Hori, Mika Hokari, Takeshi Gotoh, and Toshihiro
Sugiyama㔀⹹ฬ㧦Biomedical Research, 31(2), 155-159, 2010⊒ⴕᣣ㧦 2010 ᐕ 4 ᦬ 1 ᣣ2) ⺰ᢥ㗴ฬ㧦 Active human renin production using baculovirus expression
vector system: An effective method for preventing excessive
proteolytic degradation of recombinant proteins.
⪺⠪ฬ㧦 Takeshi Gotoh, Ken-Ichi Kikuchi, and Saori Takahashi㔀⹹ฬ㧦 Journal of Chemical Engineering of Japan, 43(7), 603-607, 2010⊒ⴕᣣ㧦 2010 ᐕ 7 ᦬ 20 ᣣ3)㩷 ⺰ᢥ㗴ฬ䋺 Yamabushitake mushroom (Hericium erinaceus) improved
lipid metabolism in mice fed a high-fat diet⪺ ⠪ ฬ 䋺 Kazuyuki Hiwatashi, Yasuyuki Kosaka, Nao Suzuki, Keishi Hata, Toshiyuki
Mukaiyama, Kenji Sakamoto, Hitoshi Shirakawa, and Michio Komai㔀⹹ฬ䋺㩷 Bioscience, Biotechnology, and Biochemistry, 74(7), 1447-1451, 2010⊒ⴕᣣ䋺 2010 ᐕ 7 ᦬ 23 ᣣ4) ⺰ᢥ㗴ฬ㧦The occurrence of renin inhibitor in rice: Isolation, identification,and structure-function relationship.
⪺⠪ฬ㧦 Saori Takahashi, Tetsuo Tokiwano, Keishi Hata, Ikuko Kodama, Mika Hokari,
Nao Suzuki, Yuko Yoshizawa, and Takeshi Gotoh㔀⹹ฬ㧦 Bioscience, Biotechnology, and Biochemistry, 74(8), 1713-1715, (2010)⊒ⴕᣣ㧦 2010 ᐕ 8 ᦬ 23 ᣣ5) ⺰ᢥ㗴ฬ䋺 Effects of lupeol on melamoma in vitro and in vivo
–Fundamental and clinical trials⪺⠪ฬ䋺Keishi Hata, Kikumi Ogihara, Saori Takahashi, Takeshi Tsuka, Saburo Minami,
Yoshiharu Okamoto㔀⹹ฬ䋺Animal Cell Technology: Basic & Applied Aspects, 16, 339-344, 2010⊒ⴕᣣ䋺 2010 ᐕ㩷 8 ᦬㩷 30 ᣣ6) ⺰ᢥ㗴ฬ䋺㩷 䈫䉐䉂⺞ᢛ㘩ຠᷝട䈮઻䈉๧⾰ᄌൻ䈱๧ⷡ䉶䊮䉰䈮䉋䉎⹏ଔ⪺⠪ฬ䋺㩷 ਛ᧛ ᗲ⟤ 1), ૒⮮ ᢥ⪇ 2), ศ↰ ᥓ 1), ᾢ⼱ ᣽ೣ 2), ㋈ᧁ 㕏ᔒ 1)1)䉰䊤䊟ᩣᑼળ␠㩷 ໡ຠ㐿⊒ᧄㇱ㩷 2) ⑺↰⋵✚ว㘩ຠ⎇ⓥᚲ㔀⹹ฬ䋺㩷 ᣣᧄ㘩ຠ⑼ቇᎿቇળ⹹䇮57(9)䇮380-388䇮2010⊒ⴕᣣ㩷 䋺㩷 2010 ᐕ 9 ᦬ 15 ᣣ7) ⺰ᢥ㗴ฬ㧦Purification and characterization of aspartic proteinase derived
from Sf9 insect cells.
⪺⠪ฬ㧦 Takeshi Gotoh, Hiroki Ono, Ken-Ichi Kikuchi, Satoru Nirasawa, andSaori Takahashi㔀⹹ฬ㧦 Bioscience, Biotechnology, and Biochemistry, 74(10), 1713-1715, (2010)⊒ⴕᣣ㧦 2010 ᐕ 10 ᦬ 23 ᣣ8) ⺰ᢥ㗴ฬ㧦Citral, a component of lemongrass oil, activates PPAR and and suppresses COX-2 expression.
⪺⠪ฬ㧦 Michiko Katsukawa, Reiko Nakata, Yoshie Takizawa, Kazuyuki Hori,
Saori Takahashi, and Hiroyasu Inoue㔀⹹ฬ㧦 Biochimica et Biophysica Acta, 1801, 1214-1220, (2010)⊒ⴕᣣ㧦 2010 ᐕ 11 ᦬ 1 ᣣ9) ⺰ᢥ㗴ฬ㧦Selective tumor imaging by a novel tumor specific aralininfrared-to-visible phosphor conjugate⪺⠪ฬ㧦Y Kawasaki, Y Gotoh, K Tokuzen, M Kamimura, T Komeno, M Tomatsu, R
Todoroki, Y Nagasaki, K Soga, and F Tashiro㔀⹹ฬ㧦Journal of Physics: Conference Series, 191, 012001 (2009)⊒ⴕᣣ㧦Online publication, 2009 ᐕ 11 ᦬ 5 ᣣ10) ⺰ᢥ㗴ฬ䋺Reduction of Blood Pressure by Soybean Saponins, Renin
Inhibitors from㩷 Soybean, in Spontaneously Hypertensive Rats⪺⠪ฬ䋺Kazuyuki Hiwatashi, Hitoshi Shirakawa, Kazuyuki Hori, Yumiko Yoshiki, Nao
Suzuki, Mika Hokari, Michio Komai and Saori Takahashi㔀⹹ฬ䋺Bioscience, Biotechnology, and Biochemistry, 74(11), 2310-2312, 2010⊒ⴕᣣ䋺 2010 ᐕ 11 ᦬ 23 ᣣ11) ⺰ᢥ㗴ฬ㧦☨ߩᚑಽ(3)࠲ࡦࡄࠢ⾰⪺⠪ฬ㧦ᄢ⢻ବਭ㔀⹹ฬ㧦㘩ຠߣኈེ‫ޔ‬51(12)‫ޔ‬720-723‫ޔ‬2010⊒ⴕᣣ㧦2010 ᐕ 12 ᦬ 1 ᣣ12) ⺰ᢥ㗴ฬ㧦Renin inhibitory activity in rice and cereals.
⪺⠪ฬ㧦 Saori Takahashi, Tetsuo Tokiwano, Nao Suzuki, Ikuko Kodama, Yuko Yoshizawa,and Takeshi Gotoh㔀⹹ฬ㧦 Journal of Biological Macromolecules, 10(3), 83-91, 2010⊒ⴕᣣ㧦 2010 ᐕ 12 ᦬ 24 ᣣ13) ⺰ᢥ㗴ฬ㩷 䋺㩷 㘩ຠ䈱ᅢ䉂⹏ଔᤨ䈱੐⽎㑐ㅪ㔚૏䈮㑐䈜䉎ታ㛎⊛ᬌ⸛⪺⠪ฬ䋺㩷 ↰ਛరᔒ 1), ᧄ㑆ᥓᄢ 1), ੗਄ᶈ 1), ᣂጊ༑༹ 2), 㜞ᯅᔀ 3), ᾢ⼱᣽ೣ 3),⑺ጊ⟤ዷ 4)1)⑺↰ᄢቇ ᄢቇ㒮Ꮏቇ⾗Ḯቇ⎇ⓥ⑼, 2)⑺↰ᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼3)⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷, 4)⑺↰⋵┙ᄢቇ ↢‛⾗Ḯ⑼ቇㇱ㔀⹹ฬ㩷 䋺㩷 㔚᳇ቇળ⺰ᢥ⹹䌃䋨㔚ሶ䊶ᖱႎ䊶䉲䉴䊁䊛ㇱ㐷⹹䋩, 131(1), 96-101 (2011)⊒ⴕᣣ㩷 䋺㩷 2011 ᐕ 1 ᦬ 1 ᣣ14) ⺰ᢥ㗴ฬ䋺㩷 ᣹࿶♽䈱ᓞㅦ㉂⚛䉕㒖ኂ䈜䉎ⴊ࿶೙ᓮ㩷 䋭䊧䊆䊮ᵴᕈ㒖ኂ䈮䉋䉎ᣂ䈚䈇䉝䊒䊨䊷䉼䋭⪺⠪ฬ䋺㩷 ᮘᷰ৻ਯ䇮㜞ᯅ⍾❱㔀⹹ฬ䋺ൻቇ䈫↢‛, 49(2), 83-85 (2011)⊒ⴕᣣ䋺 2011 ᐕ 2 ᦬ 1 ᣣ15) ⺰ᢥ㗴ฬ䋺☨♰੃㉄⊒㉂᛽಴‛䈱㜞⢽⢌㘩⽶⩄䊤䉾䊃䈮䈍䈔䉎⢽⾰⇣Ᏹᡷༀ૞↪.
⪺⠪ฬ䋺ᄢ෹ ℂት, 㜞᎑ ੝Ꮧሶ, ⩵ᳰ ⛮ᄦ, 㜞ᯅ ⚐৻㇢, ᚭᨑ ৻༑, ⇗ ᕺม㔀⹹ฬ䋺↢⮎ቇ㔀⹹, 65 (1), 33-38, 2011⊒ⴕᣣ䋺2011 ᐕ 2 ᦬㩷 20 ᣣ16) ⺰ᢥ㗴ฬ㧦 Renin inhibitor in soybean.
⪺⠪ฬ㧦 Saori Takahashi, Takeshi Gotoh, and Kazuyuki Hori㔀⹹ฬ㧦 Soybean –Biochemistry, Chemistry, and Physiology- pp389-400⊒ⴕᣣ㧦 2011 ᐕ 3 ᦬ 23 ᣣ

InTech1) ⺰ᢥ㗴ฬ㧦 Inhibition of human renin activity by saponins.
⪺⠪ฬ㧦 Saori Takahashi, Kazuyuki Hori, Mika Hokari, Takeshi Gotoh, and Toshihiro Sugiyama㔀⹹ฬ㧦Biomedical Research, 31(2), 155-159, 2010⊒ⴕᣣ㧦 2010 ᐕ 4 ᦬ 1 ᣣⷐ⚂㧦Renin is the most important enzyme in the renin-angiotensin system. Our previous study led to theidentification of soyasaponin I, the first renin inhibitor isolated from soybean. In the present study, theeffects of saponins and sapogenols on human renin activities were investigated. Soyasaponins I and II,
glycyrrhizin, monoglucuronyl glycyrrhetic acid (MGGA), chikusetsusaponin IV, and Kochia scoparia fruitsaponins (momordins) were found to inhibit renin activity. On the other hand, sapogenols (soyasapogenol B
and glycyrrhetic acid), saikosaponins b2 and c, and ginsenoside Rb1 had no effects on renin activity. These
results clearly indicate that the 3-O--D-glucopyranosiduronic moiety in saponins (glucuronide saponin) is
essential for renin inhibition.

2) ⺰ᢥ㗴ฬ㧦 Active human renin production using baculovirus expression vector system:
An effective method for preventing excessive proteolytic degradation ofrecombinant proteins.
⪺⠪ฬ㧦 Takeshi Gotoh, Ken-Ichi Kikuchi, and Saori Takahashi㔀⹹ฬ㧦 Journal of Chemical Engineering of Japan, 43(7), 603-607, 2010⊒ⴕᣣ㧦 2010 ᐕ 7 ᦬ 20 ᣣⷐ⚂㧦 Recombinant human (rh)-prorenin using the baculovirus expression vector system (BEVS) is
processed in situ to produce active rh-renin. However, rh-renin is significantly degraded during very latestage of the infection. This study aims at preventing excess degradation of recombinant proteins in the
BEVS. Culture media of baculovirus-infected Sf-9 insect cells are supplement with either proteaseinhibitors or bovine serum albumin (BSA) at various time points post infection. Although the degradationof active rh-renin is suppressed by cysteine protease inhibitors, proteolytic activation of rh-prorenin into
active rh-renin is also inhibited concurrently, and the yield of active rh-renin is decreased. On the contrary,
BSA supplementation during late stage of the infection is useful for preventing the degradation of active
rh-renin without affecting the proteolytic activation. This indicates that addition of proteins to culture
media at specific time points post infection is a simple and effective method for suppressing thedegradation of recombinant proteins expressed by the BEVS.

− 79 −

3)㩷 ⺰ᢥ㗴ฬ䋺 Yamabushitake mushroom (Hericium erinaceus) improved lipid metabolism
in mice fed a high-fat diet⪺⠪ฬ䋺Kazuyuki Hiwatashi, Yasuyuki Kosaka, Nao Suzuki, Keishi Hata, Toshiyuki Mukaiyama, Kenji
Sakamoto, Hitoshi Shirakawa, and Michio Komai㔀⹹ฬ䋺㩷 Bioscience, Biotechnology, and Biochemistry, 74(7), 1447-1451, 2010⊒ⴕᣣ䋺 2010 ᐕ 7 ᦬ 23 ᣣⷐ⚂䋺㩷 The effects of dietary Yamabushitake mushroom (Hericium erinaceus) on lipid metabolism were
examined. C57BL/6J mice were fed a high-fat diet containing hot-water extract (HW-E) and an ethanol
extract (EtOH-E) of Yamabushitake mushroom. Administration of HW-E or EtOH-E with a high-fat diet for
28 d resulted in a significant decrease in body weight gain, fat weight, and serum and hepatic
triacylglycerol levels. Our in vitro experiments indicated that EtOH-E acts as an agonist of peroxisome
proliferator-activated receptor  (PPAR). Quantitative analyses of hepatic mRNA levels revealed that
EtOH-E administration resulted in up-regulation of mRNA for a number of PPAR-regulating genes inspite of the fact that the gene expression of PPAR did not change. These results suggest that EtOH-E
improves lipid metabolism in mice fed a high-fat diet, and that these effects were mediated by modulationof lipid metabolic gene expression, at least in part via activation of PPAR.

4) ⺰ᢥ㗴ฬ㧦The occurrence of renin inhibitor in rice: Isolation, identification, and structurefunction relationship.
⪺⠪ฬ㧦 Saori Takahashi, Tetsuo Tokiwano, Keishi Hata, Ikuko Kodama, Mika Hokari,
Nao Suzuki, Yuko Yoshizawa, and Takeshi Gotoh㔀⹹ฬ㧦 Bioscience, Biotechnology, and Biochemistry, 74(8), 1713-1715, (2010)⊒ⴕᣣ㧦 2010 ᐕ 8 ᦬ 23 ᣣⷐ⚂㧦We found renin inhibitory activity in rice. The physico-chemical data on the isolated inhibitor wereidentical to those of oleic acid and linoleic acid. Oleic acid and linoleic acid competitively inhibited renin
activity, with Ki values of 15.8 and 19.8 μM respectively. Other unsaturated free fatty acids also inhibited
renin activity, but saturated fatty acids had no effects on it.

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5) ⺰ᢥ㗴ฬ䋺 Effects of lupeol on melamoma in vitro and in vivo
–Fundamental and clinical trials⪺⠪ฬ䋺Keishi Hata, Kikumi Ogihara, Saori Takahashi, Takeshi Tsuka, Saburo Minami, Yoshiharu
Okamoto㔀⹹ฬ䋺Animal Cell Technology: Basic & Applied Aspects, 16, 339-344, 2010⊒ⴕᣣ䋺 2010 ᐕ㩷 8 ᦬㩷 30 ᣣⷐ⚂䋺 Lupeol induced the dendrite formations of mouse melanoma cells by disruption of stress fiber
assembly. Western blotting revealed that inactivation of cofilin, an actin polymerization factor, caused theactin fiber disassembly in lupeol-treated melanoma cells. It has been reported that disruptions in actin fiber
assembly are involved in cancer cell motility and invasion. Therefore, we investigated the effect of lupeol
on melanoma cell motility in a cell migration assay. As the results, lupeol markedly inhibited the haptotaxisof B16 2F2 cells to fibronectin in a dose-dependent manner. Furthermore, we examined the clinical effects
of lupeol on the dogs with spontaneous melanoma. The repeat treatments with lupeol took some actions on
6 cases of 7 dogs with malignant melanomas. Especially, lupeol seemed to be more effective against themelanomas metastasizing to mandibular lymph nodes than those at primary sites.

6) ⺰ᢥ㗴ฬ䋺㩷 䈫䉐䉂⺞ᢛ㘩ຠᷝട䈮઻䈉๧⾰ᄌൻ䈱๧ⷡ䉶䊮䉰䈮䉋䉎⹏ଔ⪺⠪ฬ䋺㩷 ਛ᧛ ᗲ⟤ 1), ૒⮮ ᢥ⪇ 2), ศ↰ ᥓ 1), ᾢ⼱ ᣽ೣ 2), ㋈ᧁ 㕏ᔒ 1)1)䉰䊤䊟ᩣᑼળ␠㩷 ໡ຠ㐿⊒ᧄㇱ㩷 2) ⑺↰⋵✚ว㘩ຠ⎇ⓥᚲ㔀⹹ฬ䋺㩷 ᣣᧄ㘩ຠ⑼ቇᎿቇળ⹹䇮57(9)䇮380-388䇮2010⊒ⴕᣣ㩷 䋺㩷 2010 ᐕ 9 ᦬ 15 ᣣⷐ⚂䋺㩷 ੹࿁䈱⹜㛎⚿ᨐ䈎䉌䋬䈫䉐䉂ઃਈ䈮䉋䉎๒๧ᚑಽ䈱ᒝᐲᄌൻ䈮䈧䈇䈩䈲ቭ⢻⹏ଔ䈫๧ⷡ䉶䊮䉰䈪䈾䈿৻⥌䈜䉎䈖䈫䈏⏕⹺䈘䉏䋬๧ⷡ䉶䊮䉰䉕↪䈇䈢⹏ଔ♽䉕೑↪䈪䈐䉎䈖䈫䈏␜䈘䉏䈢䋮ቭ⢻⹏ଔ䈮ⷐ䈜䉎ᄙᄢ䈭ഭജ䈫䊌䊈䊦䈱⢻ജ䈮䉋䉎⹏ଔ䈱䈳䉌䈧䈐䈱䊥䉴䉪䉕⠨䈋䉎䈫๧ⷡ䉶䊮䉰䈮䉋䉎⹏ଔ䈲䋬䈫䉐䉂೷䈱ᕈ⢻䉕⹏ଔ䈜䉎৻䈧䈱ᚻᴺ䈫䈚䈩චಽ䈮೑↪น⢻䈪䈅䉍䋬ቭ⢻⹏ଔ䈫੕ഥ⊛䈮೑↪䈜䉎䈖䈫䈪๧⾰⹏ଔ䈱♖ᐲ䉕㜞䉄䉎䈖䈫䈏ᦼᓙ䈪䈐䉎䋮੹ᓟ䈱⺖㗴䈫䈚䈩䋬䈫䉐䉂䈏๧ᒝᐲ䈮෸䈿䈜ᓇ㗀䈮䈧䈇䈩䋬ቭ⢻⹏ଔ䈫䈱ᢛวᕈ䉕㜞䉄䉎䈖䈫䈏䈅䈕䉌䉏䉎䋮䉁䈢䋬䈫䉐䉂೷ᷝട䈮䉋䉎‛ᕈ୯䈱ᄌൻ䈫๧ⷡ䉶䊮䉰䈱಴ജᄌൻ䈫䈱㑐ଥ䉕⸃ᨆ䈜䉎䈖䈫䈮䉋䉍䋬๧䈫‛ᕈ䉕✚ว⊛䈮᝝䈋䈢㜞ᕈ⢻䈫䉐䉂೷䈱ഃ಴䈮ነਈ䈜䉎䈖䈫䈭䈬䈏䈅䈕䉌䉏䉎䋮

− 81 −

7) ⺰ᢥ㗴ฬ㧦Purification and characterization of aspartic proteinase derived from Sf9 insect cells.
⪺⠪ฬ㧦 Takeshi Gotoh, Hiroki Ono, Ken-Ichi Kikuchi, Satoru Nirasawa, and Saori Takahashi㔀⹹ฬ㧦 Bioscience, Biotechnology, and Biochemistry, 74(10), 1713-1715, (2010)⊒ⴕᣣ㧦 2010 ᐕ 10 ᦬ 23 ᣣⷐ⚂㧦An aspartic protease that is significantly produced by baculovirus-infected Spodoptera frugiperda
Sf9 insect cells was purified to homogeneity from a growth medium. To monitor aspartic protease activity,
an internally quenched fluorogenic (IQF) substrate specific to cathepsin D was used. The purified aspartic
protease showed a single protein band on SDS-PAGE with an apparent molecular mass of 40 kDa. The
N-terminal amino acid sequence of the enzyme had a high homology to a Bombyx mori aspartic protease.
The enzyme showed great affinity for the IQF substrate at pH 3.0 with a Km value of 0.85 μM. The kcat
and kcat/Km values were 13 s-1 and 15 s-1 μM-1 respectively. Pepstatin A proved to be a potent inhibitorwith inhibitor constant Ki, of 0.25 pM.

8) ⺰ᢥ㗴ฬ㧦Citral, a component of lemongrass oil, activates PPAR and  and suppresses COX-2
expression.
⪺⠪ฬ㧦 Michiko Katsukawa, Reiko Nakata, Yoshie Takizawa, Kazuyuki Hori, Saori Takahashi,
and Hiroyasu Inoue㔀⹹ฬ㧦 Biochimica et Biophysica Acta, 1801, 1214-1220, (2010)⊒ⴕᣣ㧦 2010 ᐕ 11 ᦬ 1 ᣣⷐ ⚂ 㧦 Previously, we identified carvacrol from the essential oil of thyme, as a suppressor of
cyclooxygenase-2 (COX-2), a rate-limiting enzyme for prostaglandin synthesis, expression and also
activator of nuclear receptor PPAR, a molecular target for lifestyle-related diseases. In this study, weevaluated the essential oil of lemongrass by our established assays on COX-2 and PPARs. As a result,
COX-2 promoter activity was suppressed by lemongrass oil in cell based transfection assays, and that, fromlemongrass oil, we identified citral as a major component of the suppressor of COX-2 expression and an
activator of PPARǩand ǫ. PPARǫ-dependent suppression of COX-2 promoter activity was observed in
responsetocitraltreatment.

In

human

macrophage-likeU937

cells,

citralsuppressed

lipopolysaccharide-induced COX-2 mRNA and protein expression in a dose-dependent manner,respectively. Moreover, citral induced the mRNA expression of PPAR ǩ -responsive carnitine
palmitoyltransferase 1 gene and PPARǫ-responsive FABP4 gene in the U937 cells, suggesting that citral
activates PPARǩ and ǫ, and regulates COX-2 expression through its agonistic effect on PPARǫ in apart. These results will be important in understanding the anti-inflammatory and anti-lifestyle-relateddisease properties of the lemongrass.

− 82 −

9) ⺰ᢥ㗴ฬ㧦Selective tumor imaging by a novel tumor specific aralin-infrared-to-visible phosphor
conjugate⪺⠪ฬ㧦Y Kawasaki, Y Gotoh, K Tokuzen, M Kamimura, T Komeno, M Tomatsu, R Todoroki, Y Nagasaki,
K Soga, and F Tashiro㔀⹹ฬ㧦Journal of Physics: Conference Series, 191, 012001 (2009)⊒ⴕᣣ㧦Online publication, 2009 ᐕ 11 ᦬ 5 ᣣⷐ⚂㧦Aralin is a novel cytotoxic protein from Aralia elata and selectively induces apoptosis in transformed
cells as compared to normal cells. Aralin is a lectin specific for sugar chain such as galactose and possesses
RNA N-glycosidase activity. In this study, antitumor potency of aralin was analyzed using the poly
(ethyleneglycol) (PEG)/streptavidin co-immobilized infrared-to-visible upconversion phosphors, Y2O3
nanoparticles. Cy3- conjugated aralin could clearly detect the surface of SV40-transformed VA13 andhuman cervical carcinoma HeLa cells, but to a lesser extent on the normal human fibroblast WI-38 cells.
Conjugation of aralin with PEGylated Y2O3 nanophosphor was carried out via biotin- avidin binding. The
Y2O3-conjugated aralin also clearly visualize by a fluorescence microscope measurements equipped withnear-infrared excitation source scanning in HeLa cells. It is also important to note that no remarkabledamage to the cells was observed during these observations. Thus, these data imply that the
Y2O3-conjugated aralin would potentially be useful material for tumor detection in vivo.

10) ⺰ᢥ㗴ฬ䋺Reduction of Blood Pressure by Soybean Saponins, Renin Inhibitors from㩷 Soybean,in Spontaneously Hypertensive Rats⪺⠪ฬ䋺Kazuyuki Hiwatashi, Hitoshi Shirakawa, Kazuyuki Hori, Yumiko Yoshiki, Nao Suzuki, Mika
Hokari, Michio Komai and Saori Takahashi㔀⹹ฬ䋺Bioscience, Biotechnology, and Biochemistry, 74(11), 2310-2312, 2010⊒ⴕᣣ䋺 2010 ᐕ 11 ᦬ 23 ᣣⷐ⚂䋺㩷 The effect of commercial purified soybean saponin on renin activity and blood pressure wasinvestigated. Soybean saponin significantly inhibited human renin in vitro with IC50=59.9 g/ml. Orally
administered soybean saponin at 80 mg/kg of body weight per day to spontaneously hypertensive rats for 8
weeks significantly decreased the blood pressure.

− 83 −

11) ⺰ᢥ㗴ฬ㧦☨ߩᚑಽ(3)࠲ࡦࡄࠢ⾰⪺⠪ฬ㧦ᄢ⢻ବਭ㔀⹹ฬ㧦㘩ຠߣኈེ‫ޔ‬51(12)‫ޔ‬720-723‫ޔ‬2010⊒ⴕᣣ㧦2010 ᐕ 12 ᦬ 1 ᣣ
ⷐ  ⚂㧦♖☨⾂⬿ߒߚฎ☨ߩ☨㘵࠹ࠢࠬ࠴ࡖ࡯ഠൻ߿ᡷ⦟ߦߟ޿ߡᬌ⸛ߒߚ‫ ߩ⾰ࠢࡄࡦ࠲ޕ‬SS⚿วࠍ㐿ⵚߔࠆㆶర೷ṁᶧߢἺ㘵ߔࠆߎߣߦࠃࠅ‫ޔ‬ฎ☨☨㘵ߩࡃ࡜ࡦࠬᐲ㧔☼ࠅ㧛⎬ߐ㧕߇਄᣹ߔࠆߎߣ߇ಽ߆ߞߚ‫ ߩ⾰ࠢࡄࡦ࠲ޕ‬SS ⚿วࠍ㐿ⵚߔࠆㆶరജ߇☨☸⴫ጀ࠲ࡦࡄࠢ⾰ߩ SS ⚿วߩ㐿ⵚߣ޿߁࠲ࡦࡄࠢ⾰ߩ“⾰”ߩᄌൻࠍᒁ߈⿠ߎߒ㧘ߐࠄߦ☨☸⴫ጀߩ࠺ࡦࡊࡦߩ♟ൻ߇ଦㅴߐࠇ㧘ᦨ⚳⊛ߦ☨㘵࠹ࠢࠬ࠴ࡖ࡯߇ᡷ⦟ߐࠇࠆߣ޿߁ࡔࠞ࠾࠭ࡓ߇ផ᷹ߐࠇߚ‫ޕ‬12) ⺰ᢥ㗴ฬ㧦Renin inhibitory activity in rice and cereals.
⪺⠪ฬ㧦 Saori Takahashi, Tetsuo Tokiwano, Nao Suzuki, Ikuko Kodama, Yuko Yoshizawa, andTakeshi Gotoh㔀⹹ฬ㧦 Journal of Biological Macromolecules, 10(3), 83-91, 2010⊒ⴕᣣ㧦 2010 ᐕ 12 ᦬ 24 ᣣⷐ⚂㧦 Our recent study showed the occurrence of renin inhibitory activity in nonglutinous rice. In thepresent study we screened for renin inhibitory activity in glutinous, sake, and indica rice, as well as various
cereals. Rice extracts tested in this study inhibited renin activity with IC50 values of 88-270 g/ml.
Glutinous rice extracts showed higher renin inhibitory activity than other rice extracts. On the other hand,
extracts of cereals such as buckwheat (soba), rye, and barnyard millet (hie) had renin inhibitory activity, butother cereals had no effects on renin activity. The buckwheat had the highest renin inhibitory activity in thisstudy. LC/MS analysis of rice and cereal extracts indicated that the major renin inhibitory compounds are
oleic acid and linoleic acid.

− 84 −

13) ⺰ᢥ㗴ฬ㩷 䋺㩷 㘩ຠ䈱ᅢ䉂⹏ଔᤨ䈱੐⽎㑐ㅪ㔚૏䈮㑐䈜䉎ታ㛎⊛ᬌ⸛⪺⠪ฬ䋺㩷 ↰ਛరᔒ 1), ᧄ㑆ᥓᄢ 1), ੗਄ᶈ 1), ᣂጊ༑༹ 2), 㜞ᯅᔀ 3), ᾢ⼱᣽ೣ 3),⑺ጊ⟤ዷ 4)1)⑺↰ᄢቇ ᄢቇ㒮Ꮏቇ⾗Ḯቇ⎇ⓥ⑼, 2)⑺↰ᄢቇ ᄢቇ㒮කቇ♽⎇ⓥ⑼3)⑺↰⋵✚ว㘩ຠ⎇ⓥ䉶䊮䉺䊷, 4)⑺↰⋵┙ᄢቇ ↢‛⾗Ḯ⑼ቇㇱ㔀⹹ฬ㩷 䋺㩷 㔚᳇ቇળ⺰ᢥ⹹䌃䋨㔚ሶ䊶ᖱႎ䊶䉲䉴䊁䊛ㇱ㐷⹹䋩, 131(1), 96-101 (2011)⊒ⴕᣣ㩷 䋺㩷 2011 ᐕ 1 ᦬ 1 ᣣⷐ⚂䋺 ੐⽎㑐ㅪ㔚૏䋨ERP䋩䉕ᜰᮡ䈫䈚䈢㘩ຠ䈱⹏ଔᴺ䈱᭴▽䉕⋡⊛䈫䈚䋬ᅢ䉂䈱⒟ᐲ䈏䉒䈎䈦䈩䈇䉎㘩ຠ䋨↹௝䋩䉕ឭ␜䈚䈩䋬3 Ბ㓏䈱⹏ଔ⺆䈪ᅢ䉂⹏ଔ䈘䈞䈢䈫䈐䈱 ERP 䉕᷹ቯ䈚䈢䇯䈠䈱⚿ᨐ䋬P300 ᚑಽ䈱㕙Ⓧ䋨ട▚ᐔဋᵄᒻ䈱ẜᤨ 250䌾500 ms 䈱㕙Ⓧ䋩䈲䇸䈫䈩䉅ᅢ䈐䇹䈫⹏ଔ䈚䈢㘩ຠ䈱䈫䈐䈮ᦨ䉅ᄢ䈐䈒䋬⹏ଔ䈮䉋䈦䈩⇣䈭䈦䈢䇯ᅢ䉂䈱㆑䈇䉕ᬌ಴䈪䈐䋬ERP 䉕ᜰᮡ䈫䈜䉎ᅢ䉂⹏ଔ䈱น⢻ᕈ䈏ᓧ䉌䉏䈢䇯䉁䈢䋬ട▚ᐔဋᵄᒻ䈱ẜᤨ⚂ 300 ms 䈮⃻䉏䉎䊏䊷䉪䈲↹௝ឭ␜䈫㑐ㅪ䈚䈢 P3a䋬350䌾500 ms 䈱䊏䊷䉪䈲⹏ଔ䈫㑐ㅪ䈚䈢 P3b 䈪䈅䉎䈖䈫䈏᣿䉌䈎䈫䈭䈦䈢䇯੹ᓟ䈲䋬୘ੱᏅ䈮㑐䈜䉎ᬌ⸛䉕฽䉄䋬ERP 䈫ᅢ䉂䈱㑐ㅪ䈮㑐䈜䉎䊂䊷䉺䉕⫾Ⓧ䈚䋬ᅢ䉂䈱⒟ᐲ䈏ਇ᣿䈭႐ว䈮䈧䈇䈩ኻᔕ䈪䈐䉎⹏ଔᴺ䈱ᬌ⸛䉕ㅴ䉄䉎੍ቯ䈪䈅䉎䇯14) ⺰ᢥ㗴ฬ䋺㩷 ᣹࿶♽䈱ᓞㅦ㉂⚛䉕㒖ኂ䈜䉎ⴊ࿶೙ᓮ㩷 䋭䊧䊆䊮ᵴᕈ㒖ኂ䈮䉋䉎ᣂ䈚䈇䉝䊒䊨䊷䉼䋭⪺⠪ฬ䋺㩷 ᮘᷰ৻ਯ䇮㜞ᯅ⍾❱㔀⹹ฬ䋺ൻቇ䈫↢‛, 49(2), 83-85 (2011)⊒ⴕᣣ䋺 2011 ᐕ 2 ᦬ 1 ᣣⷐ⚂䋺㩷 䊧䊆䊮䋭䉝䊮䉳䉥䊁䊮䉲䊮♽䈱ᓞㅦ㉂⚛䈪䈅䉎䊧䊆䊮䉕㒖ኂ䈚䈩ⴊ࿶䉕⺞▵䈜䉎ᣇᴺ䈮䈧䈇䈩⸃⺑䈚䈢䇯⥃ᐥ䈱႐䈪䈲䈗䈒ᦨㄭ䈮䈭䈦䈩䇮䊧䊆䊮䈱․⇣⊛䈭㒖ኂ೷䈪䈅䉎䉝䊥䉴䉨䊧䊮䈏䈗䈒ᦨㄭ䈮䈭䈦䈩૶↪䈘䉏䉎䉋䈉䈮䈭䈦䈢䇯䉝䊥䉴䉨䊧䊮䈲චᢙᐕ䈹䉍䈱ᣂ䈢䈭૞↪ᯏᐨ䉕᦭䈜䉎㒠࿶⮎䈪䈅䉍䇮ᣢሽ䈱㒠࿶⮎䈪䈲ⴊ࿶䈱䉮䊮䊃䊨䊷䊦䈏ਇචಽ䈭ᖚ⠪䉇೽૞↪䈏⃻䉏䉎ᖚ⠪䈮ኻ䈚䈩䈱᦭ലᕈ䈏ᦼᓙ䈘䉏䈩䈇䉎䇯䉁䈢䊧䊆䊮䉕㒖ኂ䈜䉎㘩ຠᚑಽ䈫䈚䈩䇮ᚒ䇱䈲䉻䉟䉵᛽಴ᶧਛ䈮㜞䈇䊧䊆䊮㒖ኂᵴᕈ䉕⷗಴䈚䈢䇯䈠䈖䈪䇮ฦ⒳䉪䊨䊙䊃䉫䊤䊐䉞䊷䈮䉋䉍㒖ኂ‛⾰䉕න㔌䈚䇮䈠䈱᭴ㅧ䉕䉸䊟䉰䊘䊆䊮䌉䈫หቯ䈚䈢䇯䈘䉌䈮 in vivo䈮䈍䈔䉎ⴊ࿶਄᣹ᛥ೙૞↪䉕ᬌ⸽䈜䉎䈢䉄䇮䉸䊟䉰䊘䊆䊮䌉䉕฽䉃Ꮢ⽼䉻䉟䉵䉰䊘䊆䊮䉕㜞ⴊ࿶⥄ὼ⊒∝䊤䉾䊃(SHR)䈮⚻ญᛩਈ䈜䉎⹜㛎䉕ⴕ䈦䈢䈫䈖䉐䇮෼❗ᦼⴊ࿶䈱᦭ᗧ䈭਄᣹ᛥ೙䈏⹺䉄䉌䉏䈢䇯− 85 −

15) ⺰ᢥ㗴ฬ䋺☨♰੃㉄⊒㉂᛽಴‛䈱㜞⢽⢌㘩⽶⩄䊤䉾䊃䈮䈍䈔䉎⢽⾰⇣Ᏹᡷༀ૞↪.
⪺⠪ฬ䋺ᄢ෹ ℂት, 㜞᎑ ੝Ꮧሶ, ⩵ᳰ ⛮ᄦ, 㜞ᯅ ⚐৻㇢, ᚭᨑ ৻༑, ⇗ ᕺม㔀⹹ฬ䋺↢⮎ቇ㔀⹹, 65 (1), 33-38, 2011⊒ⴕᣣ䋺2011 ᐕ 2 ᦬㩷 20 ᣣⷐ⚂㩷 䋺㩷 We previously developed the fermented extract of rice bran (RBFE) with Lactobacillus brevis
IFO12005. RBFE is rich in 㱏-amino butyric acid (GABA) from L-glutamic acid produced by L. brevis. In
the present study, we demonstrated that RBFE reduces the accumulations of adipose tissues and normalizes
the serum triglycerides and cholesterol level in high-fat diet fed rats. Furthermore, RBFE reduced the lipid
synthesis and secretion from HepG2 human hepatoma cells, by attenuation of the expressions of lipogenic
and cholesterologenic enzymes in the cells.

16) ⺰ᢥ㗴ฬ㧦 Renin inhibitor in soybean.
⪺⠪ฬ㧦 Saori Takahashi, Takeshi Gotoh, and Kazuyuki Hori㔀⹹ฬ㧦 Soybean –Biochemistry, Chemistry and Physiology- pp389-400 (2011)
InTech (ISBN 978-953-307-219-7)⊒ⴕᣣ㧦 2011 ᐕ 3 ᦬ 23 ᣣⷐ⚂㧦We developed efficient production of recombinant human renin in E. coli and Spodoptera frugiperda(Sf-9) insect cells. Using recombinant human renin and newly developed IQF substrate, we screened forrenin inhibitor from several foodstuffs and found renin inhibitory activity in miso originated from soybean.
The purified renin inhibitor from soybean was identified as soyasaponin I. Moreover, we investigated theeffects of various saponins and sapogenols on human renin activity and showed that glucuronide saponins,
glucuronic acid residues at the 3-hydroxyl sugar chain’s first inner position are essential for renin
inhibition.

− 86 −

‫✚⋵↰⑺ޟ‬ว㘩ຠ⎇ⓥᚲႎ๔‫ ╙ޠ‬1 ภ㨪╙ 13 ภੱฬ⚝ᒁ㧔ႎᢥ‫⎇ޔ‬ⓥࡁ࡯࠻‫⺑✚ޔ‬㧕=ภࡍ࡯ࠫ‫ޔ‬ᄥᄥሼਅ✢ߪ╩㗡⪺⠪ࠍ␜ߔ‫?ޕ‬‫ޤ޽ޣ‬
㕍ᧁᷕሶ  =?



?
⑺ጊ⟤ዷ  =㒙ㇱ㔐ሶ  =?
ᄤ㊁ᢅ㓶  =?
⍹Ꮉ੩ሶ  =?
⍹Ꮉආሶ  =?⍹Ꮉ㓶┨  =?
દ⮮ବᒾ  =?દ⮮ା⟵  =?દ⮮ ᳢  =?
ጤ㊁ำᄦ  =?
ᄢਭ଻⦟৻ =?

?
ᄢ⢻ବਭ  =ᄢ㊁ ೰  =
?ᄢਭ㐳▸  =
?
ዊ═ේඳା =
?
ዊᎹା᣿  =?
᛬ᚭ߼ߋߺ =?
የᒛ߆߅ࠆ =


?
‫ޤ߆ޣ‬ട⮮᣿ᵤሶ=?
㊄ሶ㓉ብ=
?ᧁ᧛⾆৻  =
?
ᾢ⼱᣽ೣ  =





? ᾢ⼱ ੫  =?
ዊᨋᤘ৻  =?
੹㊁␭ሶ  =?
‫ޤߐޣ‬
ᢪ⮮ਭ৻  =?

− 87 −૒‫ ⇗ ᧁޘ‬ᐽሶ =

?
૒ᷰ㜞ᥓ  =?૒⮮ᕺ㉿  =?૒⮮ ീ  =?૒⮮ᢥ⪇=?૒ή↰㓉  =?
ᩊᧄᙗᄦ  =
?
ፉ↰ାੑ  =?ㅴ⮮ ᣽  =?
⩲ේජ⑺=?
?
⩲ේ⌀ℂ  =
⩲ේਭᤐ  =?
᧖ᧄ⌀Ꮤ  =?
᧖ᧄാੱ  =?
㋈ᧁ⡡⟤  =?
‫ޤߚޣ‬
㜞↰ศਂ  =?
㜞ᯅᘮᄥ㇢ =
?
㜞ᯅశ৻  =
?
㜞ᯅ⍾❱  =?㜞ᯅ ᔀ  =?㜞ᯅ ੳ  =
?㜞ᯅ ⼾  =?
↰ญ㓉ା  =

?
↰ญ࠻ࡕሶ =?
┙⧎ᔘೣ  =?
ㄖᏈ⧷ਃ  =?
Ⴆᧄ⎇৻  =


?
ᚭᨑ৻༑  =


?
ᚭ᧻ߐ߿߆ =
?ᚭ᧻ ⺈  =
?
‫ޤߥޣ‬
ਛ↰ஜ⟤  =?

− 88 −

㐳✽᣿ᄦ  =?
ᣂ㊁⪲ሶ  =?
‫ޤߪޣ‬⇗ ᕺม  =
?⇌ጊ ⺈  =
?
ᮘᷰ৻ਯ  =
?⦁ᧁ ീ  =?
଻ಿ⟤૫  =?ၳ ৻ਯ  =
?
‫ޤ߹ޣ‬
᧻᳗㓉ม  =?
ਃᶆᐘሶ  =?ਃᶆ 㕏  =?
‫ޤ߿ޣ‬
ጊญ⺈ਯ  =?
ጊ↰ᐘ᮸  =?ጊ↰Ả৻  =?
ศ↰⍮ม=?ศ↰ ᔀ  =?
‫ޤࠊޣ‬
?  ⧯ᨋਃ㇢  =ᷰㄝ⺈ⴡ  =

?
ᷰㄝ㓉ᐘ  =
?ᷰㄝ㓷⟤=?ᷰㇱ⚛ሶ  =?
‫ޤઁޣ‬⮀ ᢥㅢ  =?Გ 㤀ำ=?ᒛ ᥙፄ=?᧘ ㉿․=?㦘 ਛሽ=?

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⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ႎ๔ⷙቯ
‫✚ޣ‬ೣ‫ޤ‬
㧝㧚⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ႎ๔ߪ‫ޔ‬㘩ຠ⎇ⓥߦ㑐ߔࠆ᏷ᐢ޿ಽ㊁ߩේ⪺⺰ᢥ㧔ႎᢥ෸߮⎇  ⓥࡁ࡯࠻㧕‫ޔ⚂ⷐߩ⸵․ޔ⺑✚ޔ‬ቇળ⊒⴫ⷐᣦ෸߮ᣢႎ⺰ᢥౣ㍳╬ࠍឝタߔࠆ‫ޕ‬ේ⪺⺰  ᢥ㧔ႎᢥ෸߮⎇ⓥࡁ࡯࠻㧕ߪ⁛ഃ⊛ߥ߽ߩߢ޽ࠅ‫ޔ‬ଔ୯޽ࠆᣂ੐ታ߿⚿⺰ࠍ฽߻߽ߩ  ߢߥߌࠇ߫ߥࠄߥ޿‫ޕ‬㧞㧚ᛩⓂ⠪ߪ‫ޔ‬ේೣߣߒߡ⑺↰⋵✚ว㘩ຠ⎇ⓥ࠮ࡦ࠲࡯ߩ⡯ຬߣߔࠆ‫ޕ‬㧟㧚⺰ᢥߩ↪⺆ߪ‫ޔ‬ේೣߣߒߡᣣᧄ⺆ߣߔࠆ‫ޕ‬‫ޣ‬ឝタ⺰ᢥߩ⒳㘃‫ޤ‬  ේ⪺⺰ᢥ㧔ႎᢥ෸߮⎇ⓥࡁ࡯࠻㧕ߣ✚⺑ߩ㧞⒳㘃ߣߔࠆ‫ޕ‬ේ⪺⺰ᢥߪ‫⺰ޔ‬ᢥߣߒߡᧂ⊒⴫ߩ߽ߩߦ㒢ࠆ‫⻠ޔߒߛߚޕ‬Ṷⷐᣦ‫ޔ‬ળ⼏⼏੐㍳ߥߤߦ⊒⴫ߒߚౝኈࠍᛩⓂߔࠆߎߣߪᅹߍߥ޿‫ޕ‬‫ޣ‬ឝタ⺰ᢥ╬ߩࡍ࡯ࠫᢙߣᵈᗧ੐㗄‫ޤ‬㧔ႎᢥ෸߮✚⺑㧕⺰ᢥ⥄り߇⁛┙ߒߡ߅ࠅ‫ޔ‬ቢ⚿ߒߚౝኈߢߥߌࠇ߫ߥࠄߥ޿‫⺰ޕ‬ᢥߩ㐳ߐߪ․ߦ㒢ቯߒߥ޿߇‫ޔ‬㧝㧜ࡍ࡯ࠫ⒟ᐲߢ޽ࠆߎߣ߇ᦸ߹ߒ޿‫ޕ‬㧔⎇ⓥࡁ࡯࠻㧕㒢ࠄࠇߚㇱಽߩ⊒⷗߿‫ޔ‬ᣂߒ޿ታ㛎ᣇᴺߥߤ‫ޔ‬ႎᢥߣߒߡߪ߹ߣ߹ࠄߥ޿߽ߩߢ
޽ߞߡ߽‫ޔ‬ႎ๔ߔࠆଔ୯ߩ޽ࠆ߽ߩ‫⺰ޕ‬ᢥߪ‫ޔ‬㧠ࡍ࡯ࠫએౝߦ߹ߣ߼ࠆߎߣ‫ޕ‬
㧔․⸵ߩⷐ⚂㧕 ࡍ࡯ࠫߦ߹ߣ߼ࠆߎߣ‫ޕ‬㧔ቇળ⊒⴫ⷐᣦ㧕㧝ࡍ࡯ࠫએౝߦ߹ߣ߼ࠆߎߣ‫ޕ‬㧔ᄖㇱ⊒⴫⺰ᢥⷐ⚂㧕ᄖㇱ⊒⴫⺰ᢥ߿⪺ᦠ╬ߦߟ޿ߡ‫⺰ޔ‬ᢥ㗴ฬ‫⪺ޔ‬⠪ฬ‫ޔ‬㔀⹹߽ߒߊߪ⪺ᦠฬ‫ޔ‬Ꮞ‫ᦨޔ‬ೋߣᦨᓟߩࡍ࡯ࠫ෸߮⊒⴫ᐕࠍ⸥タߔࠆߣߣ߽ߦ‫ࠍ⚂ⷐޔ‬㧝ࡍ࡯ࠫએౝߦ⸥タߔࠆ‫ޕ‬‫ޣ‬ክᩏ‫ޤ‬㧝㧚ේ⪺㧔ႎᢥ෸߮⎇ⓥࡁ࡯࠻㧕෸߮✚⺑ߦ㑐ߒߡߪ‫ⶄޔ‬ᢙߩ✬㓸ᆔຬߦࠃࠅߘߩ⺰ᢥߩ  ଔ୯್ᢿ߇ߥߐࠇ‫ޔ‬ឝタߩนุ߇᳿ቯߐࠇࠆ‫ޕ‬㧞㧚✬㓸ᆔຬߪ‫⺰ޔ‬ᢥߩౝኈ‫ޔ‬ᢥ┨ߥߤߦߟ޿ߡ⪺⠪ߦᡷᱜࠍഥ⸒ߒ‫⸃ߩ⟵⇼ߪ޿ࠆ޽ޔ‬  ᣿ࠍ᳞߼ࠆߎߣ߇಴᧪ࠆ‫ޕ‬㧟㧚✬㓸ᆔຬߩ⾰໧߿ᗧ⷗ߦኻߒߡ᣿⏕ߥ࿁╵߇ߥߐࠇߚ႐วߦߪ‫ޔ‬ㅦ߿߆ߦୃᱜේⓂࠍ  ឭ಴ߒߥߌࠇ߫ߥࠄߥ޿‫ޕ‬‫ޣ‬ේⓂߩᦠ߈ᣇ‫ޤ‬㧝㧚৻⥸⊛ᵈᗧ੐㗄㧦⺰ᢥߩ⸥ㅀߪᱜ⏕ࠍᦼߒ‫◲ࠅߚࠊߦ✬ోޔ‬ẖ᣿⍎ߢ޽ࠆߎߣ‫ޕ‬㧞㧚ේⓂߪ‫ޟޔ‬9QTF‫ߡ޿↪ࠍޠ‬૞ᚑߒ‫ޔ‬#❑㐳᭽ᑼߢឭ಴ߔࠆߎߣ‫ޕ‬㧟㧚ේⓂߩᦠ૕ߪ‫ޔ‬ේೣߣߒߡ᣿ᦺ૕ࠍ↪޿‫⴫ޔ‬㗴ߪ㧝㧤ࡐࠗࡦ࠻‫ᧄޔ‬ᢥߪ㧝㧞ࡐࠗࡦ࠻  ߣߒ‫ߦ߁ࠃ޿ߔ߿ߺ⺒ޔ‬᣿⍎ߦශሼߔࠆߎߣ‫ޕ‬㧠㧚ේⓂߪ‫࠻࠶࠮ࡈࠝޔ‬ශ೚ߣߥࠆߩߢ‫ޔ‬਄ਅ‫ޔ‬ᏀฝߦߪEO ߩ૛⊕ࠍ⸳ߌࠆ‫ޕ‬

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‫⺰ޣ‬ᢥߩᒻᑼ‫ޤ‬㧝㧚ႎᢥߪ‫ޔ‬ᰴߩᒻᑼࠍߣࠆ‫ޕ‬ 㧔㧝㧕ⷐ⚂‫ޔ‬㧔㧞㧕✜⸒‫ޔ‬㧔㧟㧕ታ㛎ᣇᴺ‫ޔ‬㧔㧠㧕⚿ᨐ‫ޔ‬㧔㧡㧕⠨ኤ‫ޔ‬㧔㧢㧕ᒁ↪ᢥ₂ߩ㗅ߣ  ߔࠆ‫⻢ޕ‬ㄉߪ‫ޔ‬ᢥ₂ߩ೨ߦ౉ࠇࠆ‫ޕ‬㧞㧚⎇ⓥࡁ࡯࠻ߪ‫ޔ‬ᰴߩᒻᑼࠍߣࠆ‫ޕ‬ 㧔㧝㧕✜⸒‫ޔ‬㧔㧞㧕ታ㛎ᣇᴺ‫ޔ‬㧔㧟㧕⚿ᨐߣ⠨ኤ‫ޔ‬㧔㧠㧕ᒁ↪ᢥ₂ߣߔࠆ‫ޕ‬㧟㧚✚⺑ߪ‫ߦ․ޔ‬ᒻᑼߦߎߛࠊࠄߥ޿߇‫ᦨޔ‬ೋߦⷐ⚂ࠍઃߌࠆ‫ޕ‬㧠㧚࿑⴫ߪ‫ᧄޔ‬ᢥਛߢߪ࿑㧝޽ࠆ޿ߪ⴫㧝ߥߤߣ⴫⸥ߔࠆ‫ޕ‬㧡㧚ᒁ↪ᢥ₂ߪ‫ᧄޔ‬ᢥਛߩ⹥ᒰੱฬ߿੐㗄ߩᓟߦ਄ઃ߈ዊᢥሼߢ‫⋵↰⑺ޔ‬㧝㧕‫✚߿ޔ‬㘩⎇㧞㧙㧠㧕  ߥߤߩࠃ߁ߦ⇟ภࠍઃߒ‫৻ࠍ࠻ࠬ࡝ߩߘޔ‬᜝ߒߡᒁ↪ᢥ₂ߩ㗄ߦ⸥タߔࠆ‫ޕ‬㧢㧚ᛩⓂਛߩ⺰ᢥ‫⑳ޔ‬ା‫⚿⴫⊒ᧂޔ‬ᨐߪ‫ޔ‬ᒁ↪ᢥ₂ߦ౉ࠇߕᧄᢥਛߦ᜝ᒐߢ␜ߒᒁ↪ߔࠆ‫ޕ‬㧣㧚ᧄᢥਛߦઁߩ⺰ᢥߩ⪺⠪ฬࠍᒁ↪ߔࠆ႐วߦߪ‫ޔ‬ᷙੂߩ⿠ߎࠄߥ޿㒢ࠅᆓߩߺߣߔࠆ‫ޕ‬  ⪺⠪߇㧞ฬߩ⺰ᢥߪ‫ޔ‬ਔ⠪ߩᆓࠍ૬⸥ߒ‫ޔ‬㧟ฬએ਄ߩ႐วߪ‫╩ޔ‬㗡⪺⠪એᄖࠍ‫ޠઁޟ‬  ߣ⇛⸥ߔࠆ‫ޕ‬㧤㧚ቯ⟵ࠍᔅⷐߣߔࠆ⇛ภ߿⸥ภߩ૶↪ߪᦨዊ㒢ߦߣߤ߼ࠆ‫ޔߪߦ߈ߣࠆߔ↪૶ޕ‬ೋ಴ߩ  ▎ᚲߦᱜᑼฬࠍᦠ߈‫ߡߌ⛯ޔ‬᜝ᒐౝߦ⇛ภࠍ޿ࠇࠆ‫⇛ߚ޿↪ޕ‬ภߪᢥᧃ㧔ᒁ↪ᢥ₂ߩ  ޽ߣ㧕ߦ৻᜝ߒߡ⴫␜ߔࠆ‫⴫ޔߚ߹ޕ‬㗴ߦߪ⇛ภࠍ↪޿ߥ޿‫ޕ‬‫ޣ‬ᒁ↪ᢥ₂‫ޤ‬㧝㧚ᒁ↪ᢥ₂ߦߪ‫ᧄޔ‬ᢥਛߢߩᒁ↪㗅ߦ⇟ภࠍઃߌߡ⸥タߔࠆ‫ޕ‬㧞㧚ᒁ↪ᢥ₂ߪ‫⪺ޔ‬⠪ฬ‫ޔ‬㔀⹹ฬ߽ߒߊߪ⪺ᦠฬ‫ޔ‬Ꮞ‫ޔ‬ภ‫ᦨޔ‬ೋߣᦨᓟߩࡍ࡯ࠫ‫ⴕ⊒ޔ‬ᐕ  ߩ㗅ߦ⸥タߔࠆ‫ޕ‬㧟㧚⪺⠪ฬߪ‫ޔ‬ᆓฬߣ߽⸥ߒ‫⪺ోޔ‬⠪ฬࠍ⸥タߔࠆ‫ޕ‬㧠㧚᰷ᢥ㔀⹹ߪ‫ޔࠢ࠶࡝࠲ࠗޔ‬Ꮞߪࡏ࡯࡞࠼ߣߔࠆ‫ޕ‬㧡㧚๺ᢥ⹹ฬߪ‫⑼ޔ‬ቇᛛⴚᢥ₂ㅦႎ‫ޔߚ߹ޔ‬᰷ᢥ⹹ฬߪ‫ޔ‬%JGOKECN#DUVTCEV ߿ $KQUEK  $KQVGEJPQN$KQEJGOᛩⓂⷙቯ╬ࠍෳᾖߩߎߣ‫ޕ‬‫ޣ‬න૏ߣ‛⾰ߩฬ⒓‫ޤ‬⒳‫⾰‛ߩޘ‬න૏෸߮ߘߩ↪⺆߿⸥ภߪ‫ޔ‬࿖㓙න૏♽࡮5+ OGVTKEU[UVGOࠍၮᧄߣߔࠆ‫ޕ‬Ᏹ↪⊛ߦ↪޿ࠄࠇߡ޿ࠆ‛⾰ฬߩ߁ߜ‫ޔ‬ᭂ߼ߡ૶↪㗫ᐲ߇㜞ߊ‫޿૶ޔ‬ᣇ߇࿖㓙⊛ߦ⛔৻ߐࠇߡ޿ࠆ‛⾰ฬߪ‫ޔ‬ቯ⟵ߥߒߢ૶↪ߢ߈ࠆ‫ޕ‬‫ޣ‬ቇฬ‫ޤ‬ቇฬߦߪࠗ࠲࡝࠶ࠢࠍ↪޿ࠆ‫ޕ‬ᧄⷙቯߪᐔᚑ㧝㧝ᐕ㧠᦬㧝ᣣࠃࠅᣉⴕߔࠆ‫ޕ‬ᐔᚑ㧞㧝ᐕ㧠᦬㧝ᣣ‫৻ޔ‬ㇱᡷᱜ‫ޕ‬ᐔᚑ㧞㧞ᐕ㧠᦬㧝ᣣ‫৻ޔ‬ㇱᡷᱜ

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