Gene Analysis
Cer�i�i���ion o� Cell �ine ( ��R )Recently, many cross contaminations are rising in the human cell linnes. Therefore, the certification of the cell line used in the laboratory
is expected to be essential for the trustful result of the research. TheT case is increasing that the certification of the cell line isrecommended or required in the submission of paper year by year.
STR Analysis is spotlighted as the easiest method to certificate thee cell lines. BEX provides two services of the cell line certification;
Cell IDTM System (10 Locus) as a global standard used in the national cell banks all over the world, and PowerPlex®16 System (16Locus) suitable for the confirmation of the genotype of iPS Cell (indduced pluripotent Stem Cell) and ES Cell (Embryonic Stem Cell).

�rod��� �ine��
PriiceDelivery

Certification of Cell Line 10 Locus（STR）¥18,000// Sample> 1 week

Certification of Cell Line 16 Locus（STR）¥25,000// Sample> 1 week* An additional fee to analyze DNA for the marker in the case of certificattion of unregistered cells in the cell bank.

�eli�er� �rod���
・Peak Size Observed （Excel）・allele Data （Excel）
・Spectra Data File （PDF）

�n�or���ion
・Allele matrix values may not be discriminated according to the obseerved peaks.
・The data in this analysis service should be used only for the researcch.
・Although the examinations are conducted very carefully, BEX has no responsibility along with the results.
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

Flo� �o �rder
Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・DNA Sample > 20ng Purity: 260nm/280nm＝1.8 (recommendation)
Chilled or Frozen
・Order form
II. Please send the sample and order form to us.

BEX CO.,LTD.

23

http://www.bexnet.co.jp

Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
+
TEL: +81-3-5375-1071

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

Gene Analysis
��� S������i��
Next-day reports of the sequence data and spectra data (PDF or AB1A file type) by Email.

������� �i������� S������i��
Si
Price

Avg. Analyzed �ength

Delivery

Reaction ＋ Analysis *1¥3,500/1run

600bp～800bp> 1 Business Day

preMix *2¥12,800/8run(¥1,600/1run)

600bp～800bp> 1 Business Day

Analysis *3¥1,000/1run

600bp～800bp> 1 Business Day

Analysis *3 plate ( >= 96run)¥500/1run

600bp～800bp> 4 Business Day*1 The fee turns to be ¥2,500 / run in the case that the number of unanallyzed bases is more than that of BEX QC Standard bases.
*2 Please send 8-strip tube with the mix of templates and primers. Even iff the number of the samples are not up to the eight units, the fee for 8-rununits (¥1.600/run) should be payable.
*3 The samples reacted to ABI BigDye® v1.1 or v3.1 are acceptable in thee service of Analysis. The samples should be reacted, purified, and dried up.

���i��Price
PCR Product Purification （Single Bands Only Acceptable）¥1,000/ Sample

Email with Spectra Data PDF File and AB1 File

¥1,000/ Order

CD-R Shipment with Spectra Data PDF File and AB1 File

¥2,000/ Order

Shipment of the Document of Spectra Data

¥1,000/ Order���i���� �������・Sequence Data （Text File）
・SpectraSpectra Data File
Either PDF or AB1

��������i��・Next-day reports require the arrival of the samples by noon. (Exceppt Saturday, Sunday, Holidays)
・Free reanalysis is conducted in the conditions listed below in DNA Sequencing
Sservice.
I) Analysis expected in another condition II) The spectra are uncleaar because the analysis equipment doesn’t work.
** I) requires the service of “Reaction ＋ Analysis”
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The freight of the sample to BEX is payable.

BEX CO.,�TD.

http�//www.bexnet.co.�pe-mail info�bexnnet.co.�pphone �81-3-53�5-10�1

fax �81-3-53�5-5636

24

Gene Analysis
Flo� �o �rder
Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Sample for eaach product line・Order form
II Please send the sample and order form to us.
II.
us

Chilled or Frozen

Address: 〒173
〒 3-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+�e���ion � �n�l��i�Case1: Purified Plasmid ���Total < 7kbp・・・ > 700ng
Total > 7kbp・・・ > 1μg（ > 50ng/μL）

Case2: ��� ProductPurified・・・ > 100ng
Unpurified・・・PCR Solutionn 50μL

or * Additional fee for unpurifiedd&Primer> 10pmol / run （10μM）* Universal Primer is Free in Reaction ＋Analysis

�re�i�
requires 8-strip tube units with the mix of the template and primers.
Case2: ��� ProductPlease purify the PCR productafter checking single bandss
Case1: Purified Plasmid DNA
Total < 7kbp・・・150-300ng
Total > 7kbp・・・300-500ng

100-200bp・・・1-3ng

or 200-500bp・・・3-10ng
500-1000bp・・・5-20ng&

Primer
3.2pmol / run

1000-2000bp ・・・10-40ng> 2000bp・・・20-50ng*1 Total solution should be 14μL / run in DDW.
*2 The sample numbers are labeled in the 8-strip tube.
*3 An additional fee is ¥1,000 / tube for the universal primer.

�n�l��i�
ABI BigDye® v1.1 or v3.1 reacted samples only acceptable
The sample should be reacted, purified, and dried up.

�ni�er��l �ri�erPrimerSequence 5�� 3�Primer

Sequence 5�� 3�

M13 F-1

GTAAAACGACGGCCAGSP6ATTTAGGTGACACTATAG

M13 F-2

GTTTTCCCAGTCACGAC

10F

GTTTGATCCTGGCTCA

M13 R

GGAAACAGCTATGACCATG

800R

TACCAGGGTATCTAATCC

T7

AATACGACTCACTATAGGITS1F

GTAACAAGGT(T/C)TCCGT

T3

AATTAACCCTCACTAAAGG

ITS1R

CGTTCTTCATCGATGBEX CO.,LTD.

25

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

Gene Analysis
�ri�er ��l�in�
Quick and accurate sequencing long gene.

�rod��� �ine��
PriceSingle Strand Analysis （Incl. Primers）¥20/bpDouble Strand Analysis （Incl. Primers）¥35/bp�eli�er� ���e
Our staff contacts you after the order.

�eli�er� �rod���
・Single Strand Sequence （Text File）
・Spectra Data File （PDF and AB1 File）
・Sequence obtained with each primers （Text（File））
・Synthesized Primers�n�or���ion
・The actual fee is payable in the unavoidable termination after the diiscussion with the customers.
・The sample is adequately discarded under the strict security
security, law
law, reegulations,egulations and rules.
rules
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

Flo� �o �rder
Please email orr send the order form downloaded from BEX web page withthe text file of known sequence data.
E-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Purified Plasm
mid DNＡ ［Expected Analysis Size（bp）÷500×0.5］μg
・Order form
Chilled or Frozen
II. Please send the sample and order form to us.

BEX CO.,LTD.

http://www.bexnet.co.jp

Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

26

Gene Analysis
�i�r��e �e�e��i�� �� �e��e���i��
Microbe DNA is analyzed under Rapid Identification of Microorganissms Based on Molecular Biological Method by Gene Analysis
following Japanese Pharmacopeia. Sequencing is conducted after PCRPamplification of a part of highly variable domain in the 16S
rRNA for bacteria, and of the internal transcribed spacer1 (ITS1) reegion, located between 18S rRNA and 5.8S rRNA for fungi.

�r�d��� �i�e��

Bacteria or fungi
Unclear *DNA Extraction ～PCR ～Sequencing～BLAST SearchPriceDelivery¥15,000/ Sample> 1 week¥20,000/ Sample> 1 week* Analysis in the case that whether the sample is bacteria or fungi is uncleear.

�eli�er� �r�d���・Spectra Data （PDF）
・Sequence Data (Text File）
・Result
R lt off BLAST SearchSh （The（Th mostt concordantd t topt 10 Microbe
Mi b NaN mes tot Registered
R i t d Sequence）S）
・Operation Report����r���i��
・The sample isolation are not acceptable. The isolated strains are onnly accepted.
・The ppossibly-pathogenic
y pg
strains are not acceptable.
p・2 PCRs including DNA extraction are conducted for maximum.
・The fee turns to be half of the ordinary price. In the case that the numbernof unanalyzed bases is more than that of BEX QC
Standard bases even if the clear spectra are not obtained after two sequencings.
・Detection of microbial species are not acceptable. The customer shhould detect the species by the results of BLAST research.
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

�l�l���� OrderO dPlease email orr send the order form downloaded from BEX web page withthe text file of known sequence data.
E-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636I. Please send your requests to us.

・Agar media with the isolated strain. Chilled・Oder form
II. Please send the sample and order form to us.

Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+Reference
The Japanese Pharmacopoeia 15th edition
Rapid
p Identification of Microorganismsg
Based on Molecular Biologica
g al Methodpp1741-1742 (March 31, 2006 The MHLW Ministerial Notification Noo.285)

BEX CO.,LTD.

27

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

Gene Analysis
�e��en�e �e�e��ion �� ��r�i��h� ��l�This is the sequence detection service by Straight Walk® kit. An unnknown region is cloned from the sample ten times as a large
genome size as human. The adaptor is added to the gene after the restriction enzyme treatment. PCR is conducted with the primer
designated for the known sequence. It is possible to subclone to various vectors by adding suitable restriction enzyme sites to known
primers because the sequence of the primer includes the cutting sitte for NotⅠ.

�rod��� �ine������

One-tailed Cloningg¥80,000,> 3 week

Two-tailed Cloning¥150,000> 4 week* 9 kinds of restriction enzymes (max) are used for analysis.

�eli�er� �rod���
・Unknown
Unknown Sequence （Text File）
・Operation Report�n�or���ion
・The actual fee is payable in the unavoidable termination after the discussion with the customers.
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

Flo� �o �rder
Please email orr send the order form downloaded from BEX web page withthe text file of known sequence data.
E-mailE
mail : info@bbexnet.co.jp
bexnet co jp
FAX : +81
+81-3-5375-5636
3 5375 5636

I. Please send your requests to us.

・Genome DNA
A > 2μg Chilled or Frozen
・Order form
II. Please send the sample and order form to us.

Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+ReferenceTokuji Tsuchiya, Nanako Kameya, Ikuo Nakamura
Straight Walk: A modified method of ligation-mediated genome walkking for plant species with large genome
Analytical Biochemistry 388(2009)158-160

BEX CO.,LTD.

http:��www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

28

Gene Analysis
�eno���in�
�rod��� �ine��
Price

RAPD

S
SetupFeeF

A l i FeeAnalysis
FFrom PCR

¥10,000¥600/ Sample

From PCR¥12,000¥4,000/ Sample

Only Analysis¥12,000¥1,500/ Sample

From PCR¥12,000¥5,000/ Sample

Only Analysis¥12,000¥1,500/ Sample

STR(SSR)

T-RFLP
TRFLP* In RAPD, productivity is checked by 2 PCRs and 2 electrophoreses (nonn-fluorescence) per 1 sample.
* In STR, T-RFLP, the fragment analysis is conducted with the fluorescence primers.
* The additional fee for the primers from PCR.
* In T-RFLP, the analysis from the restriction enzyme treatment is accepttable.

�eli�er� ���e
Our staff contacts you after the order.

�eli�er� �rod���
RAPD
・The
Th photos
h t off Electrophoresis
El t hi
STR, T-RFLP
・Valuable Data （Excel）
・Spectra Data （PDF)�n�or���ion
・The fee turns to be half of the ordinary price of the analysis fee in thet case that the results of the fragment analysis are not obtained.
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

Flo� �o �rder
Please email orr send the order form downloaded from BEX web page.
E-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Genome DNA
A Chilled or Frozen
・Order form
II. Please send the sample and order form to us.

BEX CO.,LTD.

29

http://www.bexnet.co.jp

Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL ++81-3-5375-1071
TEL:+81 3 5375 1071

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

Gene Analysis
��� ���in��rodu�t �ineu��e�d����de ��� ���in�
Order of the �ene li�ted in o�ti�ized ���Cuu�to�ized ��� ���in�
Orrder of the �ene not li�ted in o�ti�ized ���PricePriceDNA Extraction（Mouse swab, hair root, tissue, etc.）¥3,000～/Sample

DNA Extraction（（Mouseswab, hair root, tissue, etc.）¥3,000～/ Sample

SNP Typing Setup Fee¥5,000/ order

SNP Typing Setup Fee

Please ask

SNP Typing Analysis Fee¥1,500/ Sample・1SNP

SNP Typing Analysis Fee¥1,500/ Sample・1SNP* SNP after optimization
pis added to optimizedp
SNP list. The SNP typing
yp g isi applied
ppto the ready-madey
SNP Typing
yp g pricepin the next order.

O�ti�ized ��� �i�t ���rti�l�
Polymorphism Site

refSNP ID

ADRB3 (adrenergic, beta-3-, receptor)

Trp34Arg

rs4994

ADRB2 (adrenergic, beta-2-, receptor, suface)

Arg16Gly

rs1042713

UCP1 ((uncouplingg protein 1))

A-3826G

rs1800592

G1543A(Glu487Lys)

rs671

CYP1A1（cytochrome P450, family 1, subfamily A, polypeptide 1）

Ile462Val

rs1048943

CYP1A1（cytochrome P450, family 1, subfamily A, polypeptide 1）

Msp1

rs4646903

Met235Thr

rs699

C677T

rs1801133

GeneALDH2 (aldehyde dehydrogenase 2 family)

AGT（angiotensinogen (serpin peptidase inhibitor, clade A, member 8)）
MTHFR（5,10-methylenetetrahydrofolate reductase (NADPH)）* Optimized list is always updated in our web page.

�eli�er� �rodu�t・Operation ReportI fInfor��tionti
・Optimization may be difficult according to the sequence around SNPP.
・The fee turns to be half of the ordinary price of the analysis fee in thet case that the results of the fragment analysis are not obtained.
・The sample is adequately discarded under the strict security, law, reegulations, and rules.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

FlFlo�tot OrderO dPlease email orr send the order form downloaded from BEX web page.
E-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Genome DNA
A Chilled or Frozen
・Order form
II. Please send the sample and order form to us.

BEX CO.,LTD.

http://www.bexnet.co.jp

Address: 〒17333-0004
0004 4F 61
61-15
15 22-Chome
Chome Itabashi-kuItabashi ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

30