植物科学最前線 3: 129 ( 2012)

MpCLE ,'!

ĒŬhŲ üĠŁĸ ĠĢsĖü¶½¶½ŻŐĔĳ½ĮĶĳĜ½Åð
860-8555 ĖüÍƊƉ 2-39-1
An analysis of MpCLE genes in Marchantia polymorpha
Key words: CLE, Hormone, Peptide, Development, Marchantia polymorpha
Shinichiro Sawa, Hiroaki Honda, Ryo Tabata
Kumamoto University
Kurokami 2-39-1, Kumamoto, 860-8555, Japan
/.



 ĂĘ#ĥĝ5Øƍą!ÈƂ"1ĂĘĝĜ5ţĽ1Ō/21 CLE(CLAVATA3/Embryo-Surrounding-Region)ZYJN[c`f$ƍCe9QOEO5mÚ"ƍĨ
6"ĮĶ

!21!

,, # CLE W7]bg1 CLAVATA3 (CLV3)-tracheary element differentiation inhibitory factor (TDIF; CLE41/CLE44)$ƍőƃŖńŊ#ĎÝňļþŃŎ#5Ø1ö/!

1(Betsuyaku et al. 2011a; Miwa etal. 2009a)
 ®Ħ! CLE űx¹

AgN1HfU=Ŧ$ 100 8]SŵĴÒ0ƍ# N û"$Ń

Ŏ´"ñ21+#C>OcZYJN
CLE N_9f#ƍ12-13 8]SŵAgN21)ƍC û"AgN21

02ƍĿŷƆ5ÖƍãėZYJN[c`fŃŎ´ƍŃŎƁŐÕĦ"Ĉŏ1Ō/21(Ohyama et al. 2010)
 2)"ţ'/2ą!ĂĘµ# CLE űx¹2008ƌV_Lb<RB?"®Ħ! CLE űx¹Ś1 ƋOelkers et al.

»­1+ƍÇ!

,ŬĦ"A?

ĂĘwź#ĂĘ$ CLE ZYJN[c`f5ğ1Ō/21# CLE űx¹$=a]N`OD",»­121 ƋOelkers et al. 2008ƌƍ# CLE N_9f$
(»2!+ƍCLE ZYJN[c`fĈŏ1ÛřÜ421ě­#3ƍ#=a]N`OD5ż
Ļ!ĂĘ"¥$/!1ĮĶ

ƍV_Lb<RB?.0,j1 CLE űx¹#īŚ$!

 Ce9QOEO@S^i"$ƍCLE űx¹32 »­ƍ9R"$ 47 (Sawa et al. 2008)ƍ

CIĂĘÆŒƅ19Q;HVT", 15 # CLE űx¹
#."ƍĂĘ@S^i"$µűx¹#Ĉŏ#ŶřÝ

# CLE űx¹z421!

»­1(Miwa et al. 2009b)

»­1/ƍĂĘ"1 CLEƍµą!Ĉŏ5è,ìđ21ƍä$ƍ# CLE űx¹#¹ŬƍĈŏŬƍC>Ocxůŀ#Ŝö5ĩéĮĶ5Ŕ1

S. Sawa - 1BSJ- Revi ew 3: 129 ( 2012)

植物科学最前線 3: 130 ( 2012)0. CLE4 CLE#Ĉŏ-C>Ocxůŀ#ĮĶ"ƍě­ƍ¹űx½ĦŜÿ#ÁƇýôİķ2Ce9QOEOn"42

CLEC>Ocxůŀ#Ŝÿ5Ŕ1",ƍµ#ĮĶō

C>Ocxůª¹#µ$úŜö0ƍ

#jďª¹$(6ö/x¹

1",Ã}$ſ2,##(Kinoshita et al. 2010)ƍ

Ŭ61#$CLV3TDIF#*1CLEűx¹5ŭ#CLEűx¹#ÔŦŨí}űx¹$ĈŏĦŸÝƅ{ŕě®5ıƍ)ƍµƈŌ/2, Ĉŏĉ·®,ŕě®5ı!

.!Ĉŏ5è1
 Ce9QOEO"

4/ƍ#"!!/"ƍCe9QOEO"$32,#CLEű

4/ƍĮĶĥě±¢ƍµ0ƍĺō/,-$0Ce9QOEO5ğ#CLE/,#pà1$«ƀ1

1CLE"Ź1ĮĶ#.!ĚČ1+ƍCLEűx¹#Ĉ

ŏŜö-ƍC>Ocxůŀ#Ŝö"$ƍXdg=Dcg

ÛřGPB?$ƍø,jĻ!ŽiĂĘčĩ5Đ&1ÁƇýô1Ƌċ6#ĵĕƌä$ƍ#GPB?#űx¹ŶŘÝ#|ƍűx¹į²
$ƍT-DNAêa9fkť

ó2ƍµ¡ŏ1ŵĴÒ#HfU=ŦT-DNAêa9f¡ŏ1ē"čĩCe9QOEO#űx¹į²āƍCLEűx¹#µAgN!+ƍűx¹Ƅ¯»­!#«ƀGPB?$űx¹į²DMK=FfHg/Ã÷"

$9fMef,,ƍ1008]S¶³Ĭ

ƍµ#CLEűx¹"$/ƍĈŏĉî³ģ}5ğűx½ĦŜÿ¡ŏ1+ƍ#ē,=b8

1/ƍCLEűx¹Ŝÿ"$¶³ù1Ō/21ƍä$ƍGPB?#CLEűx¹ƍ“GPCLE“#ſƍŜÿ5Ŕ

1. MpCLE1MpCLE2
 GPB?@S^i"$ƍ2#CLE
űx¹Ś0ƍ22MpCLE1,

MpCLE2¤vƋ¬Ǝƌ2/$ƍ®Ħ!CLEűx¹ƍ2/#ŋŞ
ĞĘ$ƍŰ!YeFCf>5ƍ

C>OcZYJN".0ŃŎ´"ñ2ƍCû#CLEN_9f

ŃŎ´

ZYJNĈŏ1pà

2
 MpCLE1$TDIF>cgY"Éƍ   ¬Ǝ Ce9QOEOGPB?#CLEŀŇć
MpCLE2$ƍCLV3

É1¶!>cgY#ĲĦ!H9Y1ı¨2TDIF$ƍŮļ5æƍÔãÊŃŎ

#Ŗ5ĎÝ1Ĉŏ5è

ī/21ƋIto et al. 2006ƌ)ƍCLV3$őƃS. Sawa - 2BSJ- Revi ew 3: 130 ( 2012)

植物科学最前線 3: 131 ( 2012)ŖńŊ#ÑŃŎ#ŃŎŖĎÝ5æƍõ©¿#5Ŭ1Ĉŏ5èŌ/21

/,ƍŃŎŖ#ĎÝ5Ø1ƍĂĘ#"ï41+ƍG

PB?,£ą#Ĉŏ5è¡ŏÝŌ/2 ä$ƍMpCLE1űx¹#ŭĥěā5~ãƍ#ŕě®śÄ5ŔqƍMpCLE1#.!Ĉŏ5èŜÿ1"MpCLE1#ŭĥě".0ƍMpCLE2#Ņŧ

5ĎÝƍMpCLE2#Ā5śÄ),ß21
MpCLEűx¹ƍGPB?"$Ə#»­!#ƍtÖ#űx½ĦŜÿ2/Ə#CLEűx¹#Ĉŏ5ſăŝ1,¡ŏ1Ō1ƍ)$ƍŭĥěā5~ãƍ#.!ÔâĦęÙ"čĩ2%.

5ăŝiƍűx¹ŶŘÝ#|GPB?5ğƍtÖšŃ!Ŝÿ5ŔŌ1

2.MpWOX1 
Ce9QOEO$ƍCLV3$ƍ[_:\K=DŨª¹#WUSCHEL(WUS)űx¹#ĥě5æ1
ī/21C>OcxůŅŧ
GPB?
21/ƈĻĂĘ)»¥ăş1+"ƍG

PB?@S^ƍ&ƍESTŴ5ğƍWUS#[`e>5ëłƍh
#ŗ5×Ƌ¬ƏƌMpWOX1
űx¹$ƍ3218]Sŵ

/!1Hf

U=Ŧ5AgN1Ō/2ƍC
e9QOEO#wuschel-related homeobox13(WOX13) ø,ƈĪ£    ¬Ə Ce9QOEOGPB?#WOXŀŇćÝ5ıWOX13Ī£Ý5ı
űx¹$ƍŉœ

/,Ś

0ƍWOX13W7]bg$ø,¸Ħ!WOXŌ/21(Nardmann et al. 2009)#/ƍGPB?"h!WOXƍWOX13Ī£Ý5ı#$bgEOXc1

3.



 CLE  WOX "&+%)

2)"ƍMpCLE1#ŭĥěāĥěæāƍ&MpWOX1#ŭĥěā5~ãƍ#ŕě®5śÄ#ņĀƍMpCLE1#ŭĥěā$ŕħŃŎ#ŃŎŖæƍ2"yŕħŃŎ#ĉîśÄ2ƍĥěæā$ŕħŃŎ#ŃŎŖŭ2"yĊÂƍ&ĊÂº#ÔâģÎśÄ21ƋĠĢ/úĥŕƌ)ƍŕħŃŎ¦ũƍ#ŭĴ"ƍMpCLE1#űx¹ĥě

in situ hybridization test".0İŢ2ƋZachgo/úĥŕƌhõƍě­#3ƍMpWOX1$ŕħŃŎ#ĥěĥŕƌƍMpWOX1

śÄ2

/ƋZachgo/úĊÂ"Ź41ņĀ$ú×/2!S. Sawa - 3
BSJ- Revi ew 3: 131 ( 2012)

植物科学最前線 3: 132 ( 2012)2)#ņĀ.0ƍä$ƍMpCLE1$ƍŕħŃŎ-ĊÂ#æ5çƍŰ!GPB?#ĥĝ5Ø1Ō1

ƍ#ÈƂ"

ƍMpWOX1$MpCLE1#jďĈŏ!Ō1#$ƍƈĻĂĘ*/21.!CLEWOX5u
C>OcxůŅŧ

ĂĘ#Ŭ"yĄľ25ı¨1 ě­ƍƈĻĂĘ"$µŏ5è#CLEűx¹

ı¨21)0³!!

/,ƍ»­ƍÃ}Ř¢},Řžƍµą!ĝĜĈ

(Betsuyaku et al. 2011b)ƍZYJN[c`f#Ąū$Ã}.0jď#C>OcxůŅŧ5Řž1ƍµò#ZYJN[c`f".1Þ°5Ű"xů1

¡ŏ!1."Ŭ,Ō/21#Řž!C>OcxůŅŧ#+"ƍ2)ƍCe9QOEO5ýô$

Ã}.0jď#ª¹

 tÖƍMpCLE1!

£À



ƍúö/

#$!Ü421

ƍMpCLE2,ğƍ.0šŃ!¹űx½ĦŜÿ5Ŕqƍ

CLEC>Ocxůŀ#ƍę"ƍt)å!

"!!ƈĻĂĘ"Ã}jď#hĹ5ö/"1

1C>OcxůŅŧĮĶ#ŮĆ!1Ō1(* üĮĶ5Ŭ+1"0ƍ

lŠ"!ƍrų¶½#ċ¼oĝƍĭËÓĝƍŪĤ¶½#¶§ÐĝƍMonash¶½#John, BowmanĝƍÏÌù¾ćĝƍOsnaburueck¶½#Sabine Zachgoĝ"#±5
$0áŤġi1

#

Betsuyaku, S., Sawa, S., & Yamada, M. 2011a. The function of the CLE peptide in plant development
and symbiosis. The Arabidopsis Book. 9: e0149
Betsuyaku, S., Takahashi, F., Kinoshita, A., Miwa, H., Shinozaki, K., Fukuda, H., & Sawa, S.2011b.
Mitogen-Activated Protein Kinase regulated by the CLAVATA receptors contributes to the shootapical meristem homeostasis. Plant Cell Physiol. 52: 14-29.
Ito, Y., Nakanomyo, I., Motose, H., Iwamoto, K., Sawa, S., Dohmae, N., & Fukuda, H. 2006.
Dodeca-CLE peptides as suppressors of plant stem cell. Science 313: 842-845.
Kinoshita, A., Betsuyaku, S., Osakabe, Y., Mizuno, S., Nagawa, S., Stahl, Y., Simon, R.,
Yamaguchi-Shinozaki, K., Fukuda, H., & Sawa, S. 2010. RPK2/TOAD2 is an essential
receptor-like kinase transmitting the CLV3 signalling in Arabidopsis. Development 137:3911-3920.
Miwa, H., Kinoshita, A., Fukuda, H., & Sawa S. 2009a. Plant meristems: CLV3/ESR-related signaling
in the shoot apical meristem and the root apical meristem. J. Plant Res. 122: 31-39.
Miwa, H., Tamaki, T., Fukuda, H., & Sawa, S. 2009b. Evolution of CLE signaling. Plant Signaling &Behavior 4: 477-481.

S. Sawa - 4
BSJ- Revi ew 3: 132 ( 2012)

植物科学最前線 3: 133 ( 2012)

Nardmann, J., Reisewitz, P., & Werr, W. 2009. Discrete Shoot and Root Stem Cell-Promoting
WUS/WOX5 Functions Are an Evolutionary Innovation of Angiosperms. Mol Biol Evol. 26:
1745-1755.
Oelkers, K., Goffard, N., Weiller, G., F., Gresshoff, P., M., Mathesius, U., & Frickey, Y. 2008.
Bioinformatic analysis of the CLE signaling peptide family. BMC Plant Biology 8:1.
doi:10.1186/1471-2229-8-1
Ohyama, K., Shinohara, H., Ohnishi, M. O., & Matsubayashi, Y. 2009. A Glycopeptide regulatingstem cell fate in Arabidopsis thaliana. Nature Chem. Biol. 5: 578-580.
Sawa, S., Kinoshita, A., Betsuyaku, S., & Fukuda, H. 2008. A large family of genes that sharehomology with CLE domain in Arabidopsis and rice. Plant Signaling & Behavior 3: 1-3.

S. Sawa - 5
BSJ- Revi ew 3: 133 ( 2012)