Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Human Nucleophosmin (NPM1) monoclonal antibody
Category
Catalog No.
Applications
Reactivity

monoclonal antibody
R‐N‐001
WB, IHC, IF, IP
Human, Dog

No cross reaction: Mouse, Chicken

Immunogen information
Immunogen
Recombinant protein of human NPM1 (P06748‐1)
UniProt ID
P06748
Synonyms
B23, NP38, Numatrin
Gene ID
4869
Product information
SourceClone No.
Epitope
Isotype
Purification method
Lot No.
Concentration
BufferStorage

Mouse
9‐2.6
This clone recognized NPM (114–219), a central region of NPM1, as well asfull length NPM1 (P06748‐1), NPM1.3 (P06748‐3) and NPM1(mut‐A).
IgG1
DEAE ion‐exchange purification
001
1.0 mg/mL
50% glycerol/PBS, pH7.4, w/o sodium azide

Store at ‐20°C.

Recommended dilutions
1:1000 – 1:2000
WBIHC
1:50 – 1:200
IF
1:50 – 1:200
1:20 – 1:50
IP
Western blot

Detection of Endogenous NPM1 in Humanleukemia Cell Lines by Western Blot.
Endogenous NPM1 in HL-60 and OCI-AML3 leukemia
cells were detected by Western blotting with mouse antihuman NPM1 monoclonal antibody (Catalog # R-N-001,upper panel).

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89‐1 Enya‐cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693‐8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

ImmunofluorescenceImmunohistochemistry

Background
Nucleophosmin was originally identified as a highly phosphorylated protein in the nucleolus. NPM1 also
localizes to the nucleoplasm, and shuttles between the nucleus and the cytoplasm. NPM1 is involvedin various biological processes such as ribosome biogenesis, centrosome duplication, genome
instability and apoptosis. The overexpression of NPM1 has been observed in many types of solid tumors,
including gastric, prostate, liver and colon. Translocation in the NPM1 gene has been reported forseveral hematopoietic malignancies; for example, t(2;5)(p23;q35) in 75% of anaplastic lymphoma
kinase-positive anaplastic large cell lymphoma. Furthermore, approximately one-third of acute myeloid
leukemia (AML) patients harbor frameshift mutations in exon 12 of the NPM1 gene, resulting in thegeneration of a nuclear export signal in the C-terminal region of NPM1 and localization of the mutant
NPM1 to the cytoplasm. NPM1 type A mutation (NPM1 mut-A; TCTG insertion at the 960th nucleotide
in exon 12) may be a good molecular marker for assessing the clinical status and predicting theoutcomes in AML patients.

References for human NPM1 monoclonal antibody (R‐N‐001)
PMID:

29507312

Journal:Scientific Reports

Application:

WB, IF, IP

IF (2020):

4.380

Title:

Analysis of the oligomeric states of nucleophosmin using size exclusion chromatography.

PMID:

19052985

Journal:

Leukemia & Lymphoma

Application:

WB, IHCIF (2020):

3.280

Title:

Abnormal cytoplasmic dyslocalisation and/or reduction of nucleophosmin protein level
rarely occurs in myelodysplastic syndromes.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89‐1 Enya‐cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693‐8501, Japan
https://mabprotein.co.jp