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Human Interleukin-18 (IL-18) monoclonal antibody
Category
Catalog No.
Applications
Reactivity

monoclonal antibody
R-I-001 (clone #11-4.1)
WB, WES, IF, IP, function-blocking
HumanImmunogen information
Immunogen
Recombinant protein of human IL-18 (37-193)
UniProt ID
Q14116
Synonyms
IGIF, IL1F4
Gene ID
3606
Product information
SourceClone No.
Isotype
Epitope
Purification method
Lot No.
Concentration
BufferStorage

Mouse
11-4.1IgG2bhuman IL-18 (63-68)
DEAE ion-exchange purification
001
0.7 mg/mL
50% glycerol/PBS, pH7.4, w/o sodium azide
Store at -20°C.

Recommended dilutions
WB
1:1000 – 1:2000
WES
1:200 – 1:250
IF
1:50 – 1:200
IP
1:20 – 1:50
Capillary Western immunoassay (WES)WES analysis of the serum of adult-onset
Still’s disease patients.
IL-18 bands in 6 (43%) of 14 AOSD samples
were clearly visible and had a molecularweight corresponding to IL-1837-193 (Catalog #R-I-001)
WES is a registered trademark of ProteinSimple.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Western blot

No Cross Reaction to the Other IL-1 FamilyProteins by Western blot.
Mouse anti-human IL-18 monoclonal antibody(Catalog # R-I-001, upper panel) selectively
recognized human IL-18 from HEK-293 celllysate expressing human IL-1 family proteins.
G196, anti-DLVPR tag (G196) mAb (Catalog #R-G-001, lower panel).

Western blot

Immunoprecipitation

Immunofluorescence

Surface Plasmon Resonance analysis

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Background
This gene encodes a member of the interleukin-1 (IL-1) family of cytokines. In addition to itsrole in the inflammatory response to microbes, recent studies implicate IL-18 as an importantfactor in human autoimmune, autoinflammatory, allergic, neurological and metabolic diseases.
Similar to IL-1β, pro-IL-18 is processed by inflammatory caspase-1 or caspase-4 to yield
mature, active form (IL-1837-193).
References for human Interleukin-18 (IL-18) antibody (R-I-001)
PMID:

30615852

Journal:Archives of Biochemistry and Biophysics

Application:

WB, IF, IP, function-blocking

IF (2020):

4.013

Title:

Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal
antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18
and the neoepitope of the inflammatory caspase-cleaved active IL-18

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp