昭和62年8月5日第三種郵便物許可

令和３年4月25日発行（隔月25日発行）

第39巻

第２号

MATERIAL TECHNOLOGY
材料技術

March-April
2021
Vol. 39, No. 2
ISSN 0289-7709
CODEN: MTECFQ

材料技術研究協会
Japan Research Institute ofMaterial Technology
https://www.jrimt.jp

MATERIALTECHNOLOGY
材料技術

VOL. 39, NO. 2
March-April

Contents＜Articles＞

Effect of various conditions on the expression of structural color of
calcium carbonate
炭酸カルシウムの構造色の発現に及ぼす諸条件の影響
Risako NATSUME，Tetsuo UMEGAKI, Yoshiyuki KOJIMA*········································15

夏目 莉紗子，梅垣 哲士，小嶋 芳行*

Three drugs combined effect of antibiotics and natural antimicrobial
agents against E. coli and Candida
E. coliおよびCandidaに対する抗生物質と天然系抗菌剤の三剤併用効果
Koki TANAKA, Ikuhiro TANIDA, Satoshi OSAWA
田中 昂希, 谷田 育宏, 大澤 敏······································································21<Editorial Note>
Acceptance of Materials Technology at J-STAGE
J-STAGE 登録誌としての採択について··············································29

 

                                      ! "  #$    %$$  &   '  ()  (%    " (    *()+*)
  ,   - '    '  $ .  */00   .   $  $     $ $ '   -   '

 &!/ %$  '   $     $  -    1

-  2 1 */3    $  $   $   5 '  $ .   

$   $ -'      '4      */3

 '  $ . $  4 4' /      $   '  -
 $        '/   -  - -       $  $   4$   -  $/ 6  $  

- $           $ . '       -'   '4      $  $   4$  
'     $  $   -     /      $   $       +*       $ $     */*7 8   '

 9 $(/ ,     :        $    $ 4  - 4

9$  2 ; < =9   $   $     $$    -'   $   4' $  /  >   %  $  $    $   ?  $
$' @  9) 9*9A $$  #4  3 9*9      ! "  #$    %$$  &   ' 

()  (%    " (    *()+*)@ !"#$%&'&()*') +,-./0120345 &()(&6 &(1

/  B ( /$/㸯 ࡣࡌࡵ࡟
ᵓ㐀Ⰽ䛸䛿䠈䝁䞁䝟䜽䝖䝕䜱䝇䜽䜔䝅䝱䝪䞁⋢䛾䜘䛖䛻ᚤ⣽䛺ᵓ㐀䜢䜒䛴䛣䛸䛻䜘䜚ⓎⰍ䛩䜛⌧㇟䛷䛒䜛䠊ᵓ㐀Ⰽ䛿䛭䛾≀㉁䛾ᣢ䛴Ⰽ⣲䛾Ⰽ䛸䛿␗䛺䛳
䛯ⓎⰍ䜢䛧䠈ග䜶䝛䝹䜼䞊䜢ኻ䜟䛺䛔䠈䛛䛴㔜㔠ᒓ䜢⏝䛔䛺䛔䛸䛔䛖≉ᚩ䛜ᣲ䛢䜙䜜䜛 1)䠊⮬↛⏺䛷ᵓ㐀Ⰽ䜢♧䛩䜒䛾䛸䛧䛶䛿┿⌔䜔㈅䛾┿⌔ᒙ䛜䛒䜚䠈䛣䜜䜙䛾୺ᡂศ䛿Ⅳ㓟䜹䝹䝅䜴䝮䛷䛒䜛 1)䠊㈅䛾┿⌔ᒙ䛷䛿㛗䛥 0.5- 20䃛m䠈ཌ䛥 0.3- 1.5䃛m⛬ᗘ䛷䛒䜚䠈䛣䛾኱䛝䛥䛿ග䛾Ἴ㛗䛻㏆ఝ䛩䜛䛯䜑ග䛾ᖸ΅䜢ཷ䛡䠈ᵝ䚻䛺Ⰽ䜢䜏䜛䛣䛸䛜䛷䛝䜛1),2)䠊䜎䛯䠈஧㓟໬䜿䜲⣲䛾⌫≧⢏Ꮚ䜒ᵓ㐀Ⰽ䜢♧䛩䛣䛸䛜▱䜙䜜䛶䛔䜛䠊䛯䛸䛘䜀䠈⢏ᚄ 280nm 䛷䛿⥳䠈360nm 䛷䛿㉥⣸䛻䛺䜛䛺䛹䛾ሗ࿌䛜䜏䜙䜜䜛
3)䠊䝫䝸䝇䝏䝺䞁䛺䛹䛷䜒ྠᵝ䛾ᵓ㐀Ⰽ䜢♧䛩 4)䠊䜎䛯䠈ᵓ㐀Ⰽ䛿䠈⢏ᚄ䠈⢏Ꮚ㛫㊥㞳䠈ᒅᢡ⋡䠈㓄⨨䠈
ゅᗘ䠈⢏Ꮚ䛾Ⰽ䛚䜘䜃ᙧ≧䛻౫Ꮡ䛩䜛䛣䛸䛜▱䜙䜜䛶䛔䜛 5)䠊
Ⅳ㓟䜹䝹䝅䜴䝮䛿䠈་⸆ရ䜔䝥䝷䝇䝏䝑䜽䠈䝂䝮䛾ῧຍ๣䛺䛹䛾ศ㔝䛷ᗈ䛟฼⏝䛥䜜䛶䛚䜚䠈䜹䝹䝃䜲䝖䠈䜰䝷䝂䝘䜲䝖䛚䜘䜃䝞䝔䝷䜲䝖䛾 3 䛴䛾⤖ᬗከᙧ䛜Ꮡᅾ䛩䜛 6)䠊䛣䜜䜙 3 䛴䛾ከᙧ䛿䠈䛭䜜䛮䜜␗䛺䜛⢏Ꮚᙧែ䜢䜒䛱䠈䜹䝹䝃䜲䝖䛿⣳㗽≧䜔⳻㠃య,䜰䝷䝂䝘䜲䝖䛿ᰕ≧䠈䝞䝔䝷䜲䝖䛿⌫≧䛚䜘䜃ᯈ≧䛾⢏Ꮚᙧ≧䛸䛺䜛.䛭䛾䛯䜑,せồ䛥䜜䜛ᙧ≧䛿⏝㏵䛤䛸䛻␗䛺䜛䠊┿⌔ᒙ䛾Ⅳ㓟䜹䝹䝅䜴䝮䛾ᙧែ䛿䜰䝷䝂䝘䜲䝖䛸䛔䜟䜜䛶䛔䜛䛜䠈䜰䝷䝂䝘䜲䝖䛾ᯈ≧⤖ᬗ䜢⏕ᡂ䛥䛫䛯౛䛿䛺䛔䠊䛣䜜䛻ᑐ䛧䛶䠈䝞䝔䝷䜲䝖䛷䛿䛣䜜䜎䛷⌫≧䛚䜘䜃ᯈ≧⤖ᬗ䛾ྜᡂ䛜ሗ࿌䛥䜜䛶䛔䜛 7䠅䠊➹⪅䜙䛿䛩䛷䛻䠈Ỉ㓟໬䜹䝹䝅䜴䝮䇲஧㓟໬Ⅳ⣲⣔཯ᛂ䛻䜘䜚䝰䝜䜶䝍䝜䞊䝹䜰䝭䞁䠄MEA䠅䜢⏝䛔䜛䛣䛸䛷䠈ᚤ⣽䛺⌫≧䝞䝔䝷䜲䝖༢୍┦䛾ྜᡂ䛻䛴䛔䛶ሗ࿌䛧䛶䛔䜛 8),9)䠊䛥䜙
䛻䠈Ⅳ㓟Ỉ⣲䜹䝹䝅䜴䝮Ỉ⁐ᾮ䜢ຍ⇕䛩䜛䛣䛸䛻䜘䜚භゅᯈ≧䝞䝔䝷䜲䝖䛜⏕ᡂ䛩䜛䛣䛸䜒ሗ࿌䛧䛶
䛔䜛 10)䠊䛣䛾䜘䛖䛻䠈䝞䝔䝷䜲䝖䛿ᯈ≧䛚䜘䜃⌫≧䛾ᙧែ䜢⏕ᡂ䛩䜛䛻䛿㐺䛧䛯ᙧែ䛷䛒䜛䠊䛧䛛䛧䠈ྜᡂ䛥䜜䛯䝞䝔䝷䜲䝖䛜ᵓ㐀Ⰽ䜢♧䛧䛯౛䛿䛺䛔䠊䛭䜜䛿ᖹᆒ⢏ᚄ䛜 1䃛m ௨ୗ䛾䝞䝔䝷䜲䝖༢୍┦䛾ྜᡂ౛䛜☜❧䛥䜜䛶䛔䛺䛔䛣䛸䠈ᚤ⣽䛺⤖ᬗ䜢ᆒ୍䛻୪䜉䜙䜜䛺䛔䛯䜑䛷䛒䜛䠊๓ሗ䛻䛚䛔䛶䠈
MEA Ỉ⁐ᾮ⃰ᗘ䠈 ᗘ䜢ไᚚ䛩䜛䛣䛸䛻䜘䜚䠈ᖹ16

ᆒ⢏ᚄ 0.66䡚㻝㻚㻢㻡㽀㼙 䛾䝞䝔䝷䜲䝖༢୍┦䛾ྜᡂ䛻ᡂຌ䛧䛶䛚䜚䠈ᖹᆒ⢏ᚄ 㻝㻚㻢㻡㽀㼙 ௨ୗ䛾䝞䝔䝷䜲䝖༢୍┦䛷ᵓ㐀Ⰽ䛜Ⓨ⌧䛧䛯䠊䛧䛛䛧䠈ⓎⰍ䛾⃰䛥䜔Ⰽ䛻䜐䜙䛜䛒䛳䛯 11)䠊䛭䛣䛷,ᮏ◊✲䛷䛿䠈0.66
䃛m 䛾⢏ᚄ䛻ไᚚ䛧䛯ᴃ෇⌫≧䝞䝔䝷䜲䝖䜢⏝䛔䛶ᵓ㐀Ⰽ䜢Ⓨ⌧䛩䜛䛣䛸䜢┠ⓗ䛸䛧䠈ᵓ㐀Ⰽ䛾ⓎⰍ䛻ཬ䜌䛩䝞䝔䝷䜲䝖⤖ᬗ䛾⢏ᚄ䠈ᠱ⃮ᾮ⃰ᗘ䛚䜘䜃ᘬ䛝ୖ䛢㏿ᗘ䛾ᙳ㡪䛻䛴䛔䛶᳨ウ䜢⾜䛳䛯䠊㸰 ヨᩱ䛚䜘䜃ᐇ㦂᪉ἲ
2.1 ヨ ᩱࣂࢸࣛ࢖ࢺࡢྜᡂ࡟ࡣ㸪Ỉ㓟໬࢝ࣝࢩ࣒࢘
(⣧ᗘ 96㸣)࠾ࡼࡧࣔࣀ࢚ࢱࣀ࣮ࣝ࢔࣑ࣥ(MEA)㸪
(⣧ᗘ 99㸣)ࢆ⏝࠸㸪ࡇࢀࡽࡢヨ⸆ࡣ㛵ᮾ໬Ꮫ♫〇࡛࠶ࡿ㸬ࡲࡓ㸪Ⅳ㓟໬ࡢࡓࡵࡢ஧㓟໬Ⅳ⣲࢞ࢫ(⣧ᗘ 99.95㸣)࡟ࡣ㸪ᕮၟ఍♫〇ࢆ⏝࠸ࡓ㸬ࢹ࢕ࢵࣉࢥ࣮ࢺࡢ⁐፹࡟⏝࠸ࡓ࢔ࢭࢺࣥ(⣧ᗘ99㸣)ࡣ㛵ᮾ໬Ꮫ〇࡛࠶ࡿ㸬
2.2 ᐇ 㦂 ᪉ ἲ
2.2.1 ᴃ෇⌫≧ࣂࢸࣛ࢖ࢺࡢྜᡂ
8 mol㺃dm-3 ࡟ㄪ〇ࡋࡓ MEA Ỉ⁐ᾮ 100 ੉㸱࡟ᑐࡋ࡚Ỉ㓟໬࢝ࣝࢩ࣒࢘ࢆ 0.05 mol ῧຍࡋ㸪
0.5 mol㺃dm-3 Ỉ㓟໬࢝ࣝࢩ࣒࢘ᠱ⃮ᾮࢆㄪ〇ࡋࡓ㸬ࡇࡢᠱ⃮ᾮࡢ ᗘࢆ 55Υ࡟Ỵᐃࡋ㸪300 rpm
࡛࠿ࡃࡣࢇࡋ࡞ࡀࡽ㸪100㸣CO㸰࢞ࢫࢆ 500 cm3㺃
min-1 ࡛࢞ࣛࢫ࣮࣎ࣝࣇ࢕ࣝࢱ࣮ࢆ⏝࠸࡚ 20 ศ㛫྿ࡁ㎸ࡴࡇ࡜࡛Ⅳ㓟࢝ࣝࢩ࣒࢘ᠱ⃮ᾮࢆస〇ࡋࡓ㸬ࡇࡢᠱ⃮ᾮࢆ 10000 rpm ࡛ 2 ศ㛫㐲ᚰศ㞳ࢆ⾜࠸㸪ࡑࡢᚋࢁ㐣㸪Ὑίࡍࡿࡇ࡜࡟ࡼࡾ㸪ᴃ෇≧ࣂࢸࣛ࢖ࢺࢆᚓࡓ㸬ᚓࡽࢀࡓヨᩱࡢ࢟ࣕࣛࢡࢱࣜࢮ࣮ࢩࣙࣥࡣ㸪X ⥺ᅇᢡ㸪࣮ࣞࢨᅇᢡ
ᘧ⢏ᗘศᕸ ᐃ㸪࣐࢖ࢡࣟࢫࢥ࣮ࣉ࠾ࡼࡧ㉮ᰝᆺ㟁Ꮚ㢧ᚤ㙾㸦SEM㸧ࢆ⏝࠸࡚⾜ࡗࡓ㸬
2.2.2 ᵓ㐀ⰍࡢⓎ⌧᪉ἲヨᩱ࡟࢔ࢭࢺࣥࢆῧຍࡋ࡚ᠱ⃮ᾮ⃰ᗘࢆ 0.010.12㸣࡟ㄪᩚࡋ㸪 45kHz ࡢ㉸㡢ἼὙίჾࢆ⏝࠸࡚ࡇࡢᠱ⃮ᾮ࡟㉸㡢Ἴࢆ 5 ศ㛫↷ᑕࡋ࡚ࢥ࣮ࢸ
࢕ࣥࢢᾮ㸦ᠱ⃮ᾮ㸧ࢆస〇ࡋࡓ㸬ࢹ࢕ࢵࣉࢥ࣮
ࢺࡣ㸪㏿ࡸ࠿࡟ࢫࣛ࢖ࢻ࢞ࣛࢫᯈ(ࢫࣛ࢖ࢻࢢࣛࢫ ᯇᾉ◪Ꮚ⫼〇)ࢆᠱ⃮ᾮ࡟ᾐₕࡉࡏ࡚ᘬࡁୖࡆ㏿ᗘ 3-25cm㺃s-1 ࡛ᘬࡁୖࡆࡓ㸬ࡇࢀࢆ᭱኱ 40 ᅇ⧞ࡾ㏉ࡋ㸪ࢹ࢕ࢵࣉࢥ࣮ࢺࢆ⾜ࡗࡓ㸬ᵓ㐀Ⰽࡢ෗┿ࡣ iphone XR ࡟࡚᧜ᙳࡋࡓ㸬࡞࠾㸪࡟࡞ࡿ࡟క࠸ᵓ㐀Ⰽࡣ㸪㯤㸪㉥㸪㟷㸪⥳࡟ኚ໬⏝࠸ࡓග※ࡢⓎගࢫ࣌ࢡࢺࣝࢆᅗ㸯࡟♧ࡍ㸬ࡇࡋ࡚࠸ࡓ㸬ࡋ࠿ࡋ࡞ࡀࡽ㸪ᵓ㐀ⰍࡢⓎ⌧ࡣ᫂☜ࡢࢫ࣌ࢡࢺࣝࢆࡳࡿ࡜㟷Ⰽ࡜㯤Ⰽࡢ⤌ࡳྜࢃ࡛ࡣ࡞࠿ࡗࡓ(a)㸬ᠱ⃮ᾮ⃰ᗘ 0.04㸪0.06㸣࡛ࡏ࡛␲ఝⓑⰍ࡜࡞ࡗ࡚࠸ࡿࡇ࡜ࡀࢃ࠿ࡗࡓ㸬ࡣᵓ㐀Ⰽࡀ㸦a㸧࡜ẚ㍑ࡋ࡚⃰ࡃⓎⰍࡋ࡚࠸ࡓࡀ㸪

Intensity / -0.04㸣ࡢ࡯࠺ࡀྛⰍࡢቃ┠ࡀࡣࡗࡁࡾࡋ࡚࠾
500ࡾ㸪ࡼࡾᙉ࠸Ⓨ⌧࡛࠶ࡗࡓ㸦(b),(c)㸧
㸬ᠱ⃮ᾮ

400

⃰ᗘ 0.12㸣࡛ࡣᵓ㐀ⰍࡢⓎ⌧ࡣ୙᫂░࡜࡞ࡗ
ࡓ(d)㸬

300
200
100
0

300

400

500

600

700

Wavelength / nm

)LJ(PLVVLRQVSHFWUXPRIOLJKWVRXUFH㸱 ⤖ᯝ࠾ࡼࡧ⪃ᐹ
3.1 ᠱ⃮ᾮ⃰ᗘࡢᙳ㡪ࡲࡎ㸪ྜᡂࡋࡓᖹᆒ⢏ᚄ 0.66ȣm ࡢࣂࢸࣛ࢖
ࢺࡢ SEM ෗┿ࢆᅗ 2 ࡟♧ࡍ㸬ᖹᆒ⢏ᚄࡣ 0.66ȣ
m ࡛࠶ࡾ㸪ୖୗࡀᖹࡽ࡜࡞ࡗࡓᴃ෇⌫≧࡛࠶ࡗࡓ㸬࡞࠾㸪㛗ᚄ࡟ᑐࡍࡿ▷ᚄࡢ๭ྜࡣ 0.7 ࡛࠶ࡗࡓ㸬࡞࠾㸪X ⥺ᅇᢡ࡛ࡣࣂࢸࣛ࢖ࢺࡢ༢୍┦࡛࠶ࡿࡇ࡜ࢆ☜ㄆࡋ㸪⢏ᗘศᕸ࡛ࡣ࠿࡞ࡾศᕸ
ᖜࡢ⊃࠸ࡶࡢ࡛࠶ࡗࡓ㸬ᴃ෇⌫≧ࣂࢸࣛ࢖ࢺࢆ⏝࠸࡚ᠱ⃮ᾮ⃰ᗘࢆ
0.01㹼0.12㸣࡟ㄪᩚࡋࢹ࢕ࢵࣉࢥ࣮ࢺࢆ⾜ࡗࡓ㸬ᅗ 3 ࡟ྛᠱ⃮ᾮ⃰ᗘ࡛ࢹ࢕ࢵࣉࢥ࣮ࢺࡋࡓ
࢞ࣛࢫᯈࡢୗ࠿ࡽගࢆ↷ᑕࡋࡓ㝿ࡢ㏱㐣ගࡢ෗┿ࢆ♧ࡍ㸬࡞࠾㸪ࢹ࢕ࣉࢥ࣮ࢺᅇᩘࡣ 30 ᅇ࡜ࡋࡓ㸬ᠱ⃮ᾮ⃰ᗘ 0.01㸣࡛ࡣගࡢ୰ᚰ࠿ࡽእ

)LJ3KRWRJUDSKVRIVDPSOHVFKDQJLQJFRQFHQWUDWLRQRIVXVSHQVLRQ$YHUDJHSDUWLFOHVL]HȝP
6XVSHQVLRQFRQFHQWUDWLRQ 㸣D  E  F  G ᅗ 4 ࡟ヨᩱࡢࢫࣛ࢖ࢻ࢞ࣛࢫᯈ⾲㠃ࢆ࣐࢖ࢡࣟࢫࢥ࣮ࣉ࠾ࡼࡧ SEM ほᐹࡋࡓ෗┿ࢆ♧ࡍ㸬ࡲࡎ㸪࣐࢖ࢡࣟࢫࢥ࣮ࣉ෗┿࡛ࡣ㸪ᠱ⃮ᾮ⃰ᗘࡢቑ኱࡟క࠸㸪࢞ࣛࢫᯈୖࡢ⢏Ꮚࡀከࡃ࡞ࡗ࡚࠸
ࡿࡢࡀ᫂☜࡟ほᐹࡉࢀࡓ(a)-(d))㸬ࡋ࠿ࡋ࡞ࡀ
ࡽ㸪0.12㸣࡛ࡣⓑ࠸㒊ศࡀከࡃほᐹࡉࢀ㸪ࡇࢀࡣ⤖ᬗྠኈࡀ㔜࡞ࡗ࡚࠸ࡓࡓࡵ࡛࠶ࡿ(d)㸬ࡉࡽ࡟㸪ヲ⣽࡟ほᐹࡍࡿࡓࡵ㸪SEM ほᐹࢆ⾜ࡗࡓ㸬
SEM ෗┿ࢆࡳࡿ࡜㸪ᠱ⃮ᾮ⃰ᗘ 0.01㸣࡛ࡣ㸪ࣂࢸࣛ࢖ࢺ⤖ᬗࡢ࢞ࣛࢫᯈ࡬ࡢ௜╔ࡀ㠀ᖖ࡟ᑡ࡞࠿ࡗࡓ㸬ࡇࡢࡓࡵ㸪⢏Ꮚ࡟ࡼࡿගࡢᖸ΅ࡀῶᑡࡋ㸪ࡇࢀ࡟ࡼࡾᵓ㐀Ⰽࡀⷧ࠸࡜⪃࠼ࡽࢀࡿ(e)㸬ᠱ⃮ᾮ⃰ᗘ 0.04㸣࡛ࡣ㸪⤖ᬗࡢ௜╔ࡀቑ1 ȣP࠼㸪ගࡢᩓ஘ࡀቑຍࡋ㸪ࡑࢀ࡟ࡼࡾᵓ㐀Ⰽࡀ⃰
ࡃⓎ⌧ࡋࡓ࡜⪃࠼ࡽࢀࡿ(f)㸬ࡋ࠿ࡋ㸪ᠱ⃮ᾮ⃰)LJ6(0SKRWRJUDSKRIVSLQGOHVSKHULFDOYDWHULWHZLWKDYHUDJHSDUWLFOHVL]HRIȝP

ᗘ 0.06㸣࡛ࡣ௜╔ࡋࡓ⢏Ꮚࡀⴠୗࡋ࡚ࡋࡲࡗ
ࡓࡢ࠿㸪⢏Ꮚᩘࡣ 0.04㸣ࡼࡾᑡ࡞ࡃ࡞ࡗ࡚࠸ࡓ
17

(g)㸬ᠱ⃮ᾮ⃰ᗘ 0.12㸣࡛ࡣ⤖ᬗࡀ✚ࡳ㔜࡞ࡗࡃ࡞ࡗࡓ(d)㸬

࡚จ㞟య࡜ࡋ࡚࢞ࣛࢫᯈ࡟௜╔ࡋ࡚࠸ࡓ(h)㸬

ᅗ 6 ࡟ྛࢹ࢕ࢵࣉࢥ࣮ࢺᅇᩘࢆ⾜ࡗࡓࢫࣛ࢖

ࡍ࡞ࢃࡕ㸪จ㞟యࡀගࡢ㏱㐣ࢆጉࡆࡿࡇ࡜࡟ࡼ

ࢻ࢞ࣛࢫᯈ⾲㠃ࡢ࣐࢖ࢡࣟࢫࢥ࣮ࣉ࠾ࡼࡧ SEM

ࡾ㸪ᵓ㐀Ⰽࡀⷧࡃ࡞ࡗࡓ࡜⪃࠼ࡽࢀࡿ㸬ほᐹࡋࡓ෗┿ࢆ♧ࡍ㸬1 ᅇࢹ࢕ࢵࣉࢥ࣮ࢺࡋࡓ
ヨᩱ࡛ࡣࣂࢸࣛ࢖ࢺ⤖ᬗࡀࢫࣛ࢖ࢻ࢞ࣛࢫ࡟
ࡲࡤࡽ࡟௜╔ࡋ࡚࠸ࡿࡢࡀほᐹࡉࢀࡓ(e)㸬ࡑࡢࡓࡵ㸪ගࡢᖸ΅ࡀῶᑡࡋ࡚㸪ᵓ㐀ⰍࡢⓎ⌧ࡀᙅ࠿ࡗࡓ࡜᥎ᐹࡋࡓ㸬10 ᅇ⾜ࡗࡓヨᩱ࡛ࡣ 1 ᅇ⾜ࡗࡓヨᩱࡼࡾࡶከࡃࡢ⤖ᬗࡀ௜╔ࡋ࡚࠸ࡓࡀ㸪⤖ᬗࡀ௜╔ࡋ࡚࠸࡞࠸⟠ᡤࡶࡳࡽࢀ㸪⤖ᬗ

)LJ 6(0 DQG RSWLFDO PLFURVFRSH LPDJHV RI
VDPSOHV V\QWKHVL]HG FKDQJLQJ FRQFHQWUDWLRQ RI
VXVSHQVLRQ$YHUDJH SDUWLFOH VL]H ȝP
6XVSHQVLRQFRQFHQWUDWLRQ 㸣
D  H  E  I  F  J  G  K ࡢ௜╔ࡢ௙᪉ࡀ୙ᆒ୍࡛࠶ࡗࡓ(f)㸬30 ᅇ⾜ࡗࡓヨᩱ࡛ࡣఱࡶ௜╔ࡋ࡚࠸࡞࠸㒊ศࡶ࠶ࡗࡓ
ࡀ࠿࡞ࡾ⦓ᐦ࡟⤖ᬗࡀ௜╔ࡋ࡚࠸ࡓ(g)㸬ࡑࡢࡓࡵ㸪ᵓ㐀ⰍࡢⓎ⌧ࡀᙉࡲࡗࡓ࡜⪃࠼ࡽࢀࡿ㸬40 ᅇ⾜ࡗࡓヨᩱ࡛ࡣ⤖ᬗྠኈࡀ㔜࡞ࡗࡓ㒊ศ

3.2 ࢹ࢕ࢵࣉࢥ࣮ࢺᅇᩘࡢᙳ㡪ࡀከࡃ㸪ࡑࢀ࡟ࡼࡾ஘཯ᑕࡋ࡚ᵓ㐀Ⰽࡀⷧࡃ࡞

ᖹᆒ⢏ᚄ 0.66ȣm ࡢᴃ෇≧ࣂࢸࣛ࢖ࢺࢆ⏝࠸ࡗࡓ࡜⪃࠼ࡽࢀࡿ(h)㸬࡚㸪ᵓ㐀ⰍࡢⓎ⌧࡟ཬࡰࡍࢹ࢕ࢵࣉࢥ࣮ࢺᅇᩘ
ࡢᙳ㡪࡟ࡘ࠸᳨࡚ウࡋࡓ㸬ᅗ 5 ࡟ྛࢹ࢕ࢵࣉࢥ
࣮ࢺᅇᩘ࡟࠾࠸࡚ほᐹࡉࢀࡓᵓ㐀Ⰽࡢ෗┿ࢆ♧ࡍ㸬1 ᅇࢹ࢕ࢵࣉࢥ࣮ࢺࡋࡓヨᩱ࡛ࡣ඲యⓗ࡟㯤Ⰽ࠸ᵓ㐀Ⰽࡀほᐹࡉࢀࡓ(a)㸬10 ᅇ⾜ࡗࡓヨᩱ࡛ࡣ 1 ᅇࡢヨᩱࡼࡾࡶ㯤Ⰽ࿡ࡀᙉࡃⓎ⌧ࡋ࡚࠸ࡓ(b)㸬ࡉࡽ࡟㸪ᅇᩘࢆ㔜ࡡ࡚ 30 ᅇ⾜ࡗࡓ

)LJ 6(0 DQG RSWLFDO PLFURVFRSH LPDJHV RI VDPSOHVV\QWKHVL]HG FKDQJLQJ FRQFHQWUDWLRQ RI 1XPEHU
RIGLSFRDWV$YHUDJHSDUWLFOHVL]HȝP
1XPEHURIGLSFRDWVWLPHVD  H  E  I  F  J  G  K ヨᩱ࡛ࡣ㸪ෆഃ࠿ࡽ㯤㸪㉥㸪⣸ࡑࡋ࡚⥳ࡢ㡰࡟ᵓ㐀Ⰽࡀᙉࡃほᐹࡉࢀࡓ(c)㸬ࡋ࠿ࡋ㸪40 ᅇࢹ࢕ࢵࣉࢥ࣮ࢺࡋࡓヨᩱࡣᵓ㐀Ⰽࡀࢃࡎ࠿࡟ⷧ

3.3 ᵓ㐀ⰍࡢⓎ⌧࡟ཬࡰࡍࢹ࢕ࢵࣉࢥ࣮ࢺ㏿ᗘࡢᙳ㡪ࡘࡂ࡟㸪ᵓ㐀ⰍࡢⓎ⌧࡟ཬࡰࡍࢹ࢕ࢵࣉࢥ࣮
ࢺ㏿ᗘࡢᙳ㡪࡟ࡘ࠸᳨࡚ウࢆ⾜ࡗࡓ㸬ᅗ 7 ࡟ྛᖹᆒ㏿ᗘ࡛ࢹ࢕ࢵࣉࢥ࣮ࢺࡋࡓヨᩱࡢ෗┿ࢆ♧ࡍ㸬࠸ࡎࢀࡢヨᩱࡶࢹ࢕ࢵࣉࢥ࣮ࢺᅇᩘࡣ 30ᅇ࡛࠶ࡿ㸬᭱ࡶ㏿ᗘࡀ㏿࠸ᖹᆒ㏿ᗘ 25 cm㺃s-1
࡛ࡣ⤖ᬗ࡝࠺ࡋࡢ㔜࡞ࡾࡣࡳࡽࢀࡎ㸪⤖ᬗࡀᆒ୍࡟௜╔ࡋ࡚࠸ࡿࡢࡀほᐹࡉࢀ㸪ࡲࡓෆഃ࠿ࡽ㸪㯤㸪㉥㸪㟷㸪⥳ࡢᵓ㐀Ⰽࡀ⃰ࡃⓎⰍࡋࡓ(a)㸬ᖹᆒ㏿ᗘ 5.0 cm㺃s-1 ࡟࡞ࡿ࡜㸪⤖ᬗࡀ㔜࡞ࡗࡓⓑ࠸➽ࡀࡳࡽࢀࡿࡼ࠺࡟࡞ࡗࡓ㸬ࡲࡓ㸪ෆഃ࠿

)LJ3KRWRJUDSKVRIVWUXFWXUDOFRORUGHYHORSHGDWHDFK1XPEHURIGLSFRDWV$YHUDJHSDUWLFOHVL]HȝP
1XPEHURIGLSFRDWVWLPHVD  E  F  G ࡽ㸪㉥㸪㯤㸪㟷ࡢᵓ㐀Ⰽࡀ☜ㄆ࡛ࡁࡓ(b)㸬ࡋ࠿ࡋ࡞ࡀࡽ㸪ྛⰍࡢቃ┠ࡀ᭕᫕࡟࡞ࡗࡓ㸬᭱ࡶ㐜࠸ᖹᆒ㏿ᗘ 3.3 cm㺃s-1 ࡛ࡣ㸪ⓑ࠸➽ࡀከࡃほᐹ
18

Observer

green
53r
blue
55r
red
61r yellow
65r

)LJ

3KRWRJUDSKV RI VDPSOHV FKDQJLQJ DYHUDJH
VSHHG
$YHUDJHSDUWLFOHVL]HȝP$YHUDJHVSHHGFP࣭V
D  E  F spindle spherical vaterite
light)LJ ,QFLGHQWDQJOHRIHDFKVWUXFWXUDOFRORU

ࡉࢀ㸪⫗║࡛ࡶ⤖ᬗࡀ㔜࡞ࡗ࡚࠸ࡿࡇ࡜ࡀㄆ㆑
ࡉࢀ㸪ᵓ㐀Ⰽࡶⷧࡃ࡞ࡗࡓ(c)㸬
ࡘࡂ࡟㸪ࡇࢀࡽࡢヨᩱࡢࢫࣛ࢖ࢻ࢞ࣛࢫᯈࡢ

࡟╔┠ࡋ㸪㏆ఝⓗ࡟ⷧ⭷ᖸ΅⌮ㄽࢆ⏝࠸࡚ゎᯒ⾲㠃ࡢ࣐࢖ࢡࣟࢫࢥ࣮ࣉ࠾ࡼࡧ SEM ෗┿ࢆᅗ 8

ࢆヨࡳࡓ㸬ගࡢᖸ΅ࡀᙉࡵྜ࠺ᘧࢆ

࡟♧ࡍ㸬ᖹᆒ㏿ᗘࡀ㐜ࡃ࡞ࡿ࡯࡝⤖ᬗࡀ㔜࡞ࡿ㒊ศࡀቑຍࡋࡓ㸬ᖹᆒ㏿ᗘ:25㸪5.0 cm㺃s-1 ࡛ࡣ

2dncosȟ=(m +1/2)Ȣ

⤖ᬗࡀ 1 ᒙࡲࡓࡣ 2 ᒙ࡟୪ࢇ࡛࠸ࡓ(d)㸬୍᪉㸪ᖹᆒ㏿ᗘ:3.3 cm㺃s-1 ࡛ࡣ⤖ᬗࡀ 3 ࡘ௨ୖ㔜࡞࡛⾲⌧ࡍࡿࡇ࡜ࡀ࡛ࡁࡿ㸬ࡇࡇ࡛ n㸸ᒅᢡ⋡㸪ࡗ࡚࠸ࡿ㒊ศࡀከࡃ㸪ࡇࡢࡓࡵྍどගࡢἼ㛗࠿d:ཌࡳ㸪Ȣ㸸Ἴ㛗࡛࠶ࡾ㸪m㸸ᩚᩘ㸪ȟ㸸ධᑕゅࡽ⢏ᚄࡀ኱ࡁࡃእࢀ㸪ගࡀᩓ஘ࡋ࡚ᵓ㐀Ⰽࡀࡳ࡛࠶ࡿ㸬ࣂࢸࣛ࢖ࢺࡢᒅᢡ⋡ࡣ 1.56㸫1.66 ࡛࠼࡟ࡃࡃ࡞ࡗࡓ࡜⪃࠼ࡽࢀࡿ(f)㸬

࠶ࡾ㸪1.58 ࡜ࡋ࡚ィ⟬ࢆ⾜ࡗࡓ㸬ཌࡳࡣ⤖ᬗࡀ㛗ᚄ㸸▷ᚄ㸻1㸸0.7 ࡢᴃ෇⌫≧࡛࠶ࡿࡇ࡜࠿ࡽ

3㺃4 ᵓ㐀ⰍࡢⓎ⌧ᶵᵓ

6600.7㸻462 nm ࡜ࡋࡓ㸬ࡲࡓ㸪m 㸻㸯࡜ࡋ௒ᅇࡢ◊✲࡟࠾࠸࡚ᴃ෇⌫≧ࣂࢸࣛ࢖ࢺ⤖ࡓ㸬㯤Ⰽࡢධᑕゅ cosȟ=65r࡜ࡋ࡚ィ⟬ࢆࡍࡿᬗࢆ୪࡭࡚㸪ࡑࡇ࡟ගࢆ↷ᑕࡍࡿ࡜㏱㐣ග࡛ᵓ࡜Ἴ㛗ࡣ 433nm ࡜࡞ࡗࡓ㸬ࡇࢀࡣ㟷ⰍࡢἼ㛗࡛㐀Ⰽࡀほᐹࡉࢀࡓ㸬ࡑࡢⰍࡣほᐹഃ࠿ࡽ㯤㸪㉥㸪࠶ࡾ㸪ࡑࡢ⿵Ⰽࡢ㯤Ⰽࡀ㏱㐣ග࡜ࡋ࡚ⓎⰍࡍࡿ㸬㟷㸪⥳࡜࡞ࡗࡓ㸬ᅗ 9 ࡟ᵓ㐀Ⰽࡢධᑕゅ࡜ⓎⰍ㉥㸪㟷㸪⥳ࡢධᑕゅ࡛ィ⟬ࡍࡿ࡜㸪㉥Ⰽࡢධᑕࢆ♧ࡍ㸬

ゅȟ=61 ࡢ࡜ࡁ 497nm㸪㟷ⰍࡢධᑕゅධᑕゅȟྛⰍࡢධᑕゅࡣ㯤ࡀ 65r㸪㉥ࡀ 61r㸪㟷ࡀ=55rࡢ࡜ࡁ 588nm,⥳Ⰽࡢධᑕゅȟ=53rࡢ࡜

55r㸪⥳ࡀ 53r࡛࠶ࡗࡓ㸬௒ᅇࡣ㸪࣑࣮ᩓ஘ࡢ

ࡁ 617nm ࢆ♧ࡋࡓ㸬ࡑࢀࡒࢀࡢἼ㛗ࡢ⿵Ⰽࡣ㉥㸪ࡼ࠺࡞ගᩓ஘⌧㇟ࡀ㛵ಀࡋ࡚࠸ࡿྍ⬟ᛶࡶ࠶㟷㸪⥳Ⰽ࡛࠶ࡾ㸪⫗║࡛ほᐹࡋࡓⰍ࡜࡯ࡰ୍⮴ࡿࡀ㸪ᴃ෇⌫≧ࡢ⢏Ꮚࡀᒙࢆᙧᡂࡋ࡚࠸ࡿࡇ࡜

ࡋࡓ㸬㸲㸬࠾ࢃࡾ࡟
ᮏ◊✲࡛ࡣ㸪MEA ࢆ⏝࠸࡚ྜᡂࡋࡓᖹᆒ⢏ᚄ
0.66m ࡢᴃ෇⌫≧ࣂࢸࣛ࢖ࢺࢆ⏝࠸࡚㸪ᵓ㐀ⰍࡢⓎ⌧࡟ࡘ࠸᳨࡚ウࢆ⾜ࡗࡓ㸬⤖ᬗࡀ㛗ᚄ㸸▷ᚄ㸻1㸸0.7 ࡢᴃ෇⌫≧࡛࠶ࡿࡇ࡜࠿ࡽ㸪ᖹᆒ⢏ᚄ㹼0.7ᖹᆒ⢏ᚄ࡟‽ࡎࡿⰍࡀほᐹ࡛ࡁࡓ㸬ࢫࣛ࢖ࢻ࢞ࣛࢫ࡟௜╔ࡋࡓ⤖ᬗࡀᑡ࡞࠸࡜㸪㏱
㐣ࡍࡿගࡀῶᑡࡋᵓ㐀Ⰽࡀⷧࡃ࡞ࡾ㸪ఱᒙ࡟ࡶ㔜࡞ࡗ࡚௜╔ࡍࡿ࡜ྍどගࡢἼ㛗࠿ࡽ⢏ᚄࡀ኱ࡁࡃእࢀ㸪ගࡀศᩓࡋᵓ㐀Ⰽࡀⷧࡃ࡞ࡗࡓ㸬)LJ6(0DQGRSWLFDO PLFURVFRSH LPDJHVRIVDPSOHVFKDQJLQJDYHUDJHVSHHG
$YHUDJHSDUWLFOHVL]HȝP$YHUDJHVSHHGFP࣭V
D  G  E  H  F  I 19

 Ᏺྜྷభ௓͆↓ᶵᮦᩱᚲ㡲 300-ཎ⌮㺃≀ᛶ㺃ᛂࡑࡇ࡛㸪ᵓ㐀Ⰽࡢ⌧᫬Ⅼ࡛ࡢ᭱㐺࡞ࢹ࢕ࢵࣉࢥ
࣮ࢺ᮲௳ࡣ㸪
ᠱ⃮ᾮ⃰ᗘ 0.04㸣㸪
ᖹᆒ㏿ᗘ 25cm㺃

⏝-͆㸪୕ඹฟ∧,2008,p.477-500.

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E. coli ࠾ࡼࡧ Candida ࡟ᑐࡍࡿᢠ⏕≀㉁࡜ኳ↛⣔ᢠ⳦๣ࡢ୕๣ే⏝ຠᯝ⏣୰ ᪸ᕼ ㇂⏣ ⫱ᏹ ኱⃝ ᩄ㔠ἑᕤᴗ኱Ꮫ ࢤࣀ࣒⏕≀ᕤᏛ◊✲ᡤ, ࠛ924-0838 ▼ᕝ┴ⓑᒣᕷඵ᮰✑ 3-1

7KUHHGUXJVFRPELQHGHIIHFWRIDQWLELRWLFVDQGQDWXUDODQWLPLFURELDODJHQWVDJDLQVW(FROLDQG&DQGLGD.RNL7DQDND,NXKLUR7DQLGD6DWRVKL2VDZDGenomu Biotechnology Laboratory, Kanazawa Institute of Technology,
Yatsukaho, Hakusan, Ishikawa, 924-0838
E-mail㸸b6900980@planet.kanazawa-it.ac.jp

Abstract
The three drugs combined effects of various antibiotics and natural antimicrobial substances, Benzyl
isothiocyanate (BITC), Cinnamaldehyde (CAL) have been investigated against Escherichia coli (E. coli)
and Candida parapsilosis (C. parapsilosis). Antimicrobial activities were determined by minimum
inhibitory concentration (MIC) using micro-dilution method. The combination effect was judged by thefractional inhibitory concentration (FIC) index using the checkerboard method. Microencapsulation was
performed by a submerged curing coating method in which a mixture of sodium alginate and an antibiotic
or antibacterial agent was reacted with calcium chloride to form capsules by ionic cross-linking. The
results showed that the combination of Polymyxin B and BITC, CAL had combined effect against E. coli.
The combination of Amphotericin B, BITC and CAL showed combined effects on C. parapsilosis. MIC
value of three drags combination is much lower than that of two drags combination, leading to reductionof antibiotic usage. Furthermore, sustained release of antimicrobial agents from microcapsules was
conformed in vitro experiment.

.H\ZRUGV㸸$QWLELRWLFV1DWXUDODQWLPLFURELDODJHQW&RPELQHGHIIHFW0LFURFDSVXOH
Received February 6, 2021; accepted March 1, 2021

21

㸯 ⥴ゝ
2019 ᖺ࡟࠾ࡅࡿ඲ୡ⏺ࡢ୺࡞Ṛᅉࡣ, ⹫⾑➼ࡢᵝࠎ࡞᮲௳タᐃ࡟ࡼࡾ, ⸆๣ࡢᚎᨺࡀ␗࡞

ᛶᚰ⑌ᝈࡸ⬻༞୰, ⫵⑌ᝈ࡞࡝ࡢ㠀ឤᰁ⑕࡛࠶

๣ే⏝ຠᯝࢆ♧ࡋࡓᢠ┿⳦⸆, BITC, CAL ࢆࡿࡀ, పᡤᚓᅜࡢṚᅉ TOP10 ࡢ࠺ࡕ, 6 ࡘࡣឤ

MC ໬ࡉࡏ, ࡑࡢ⸆๣ࡢ⁐ฟᣲືࢆ᳨ウࡋࡓ.

ᰁ⑕࡛࠶ࡿࡿᣲືࢆ♧ࡍࡇ࡜ࡶ▱ࡽࢀ࡚࠸ࡿ. ࡑࡇ࡛, ୕1). ࡲࡓ, ㏆ᖺ࡛ࡣ,⸆๣⪏ᛶ⳦ࡢᝈ⪅ᩘࡶቑຍഴྥ࡟࠶ࡾ, ᑐ⟇ࡀồࡵࡽࢀ࡚࠸ࡿ.
⸆๣⪏ᛶ⳦࡟ᑐࡋ࡚, ୡ⏺ⓗ࡞ᑐᛂࡀ⾜ࢃࢀ࡞2 ᐇ㦂᪉ἲ
ᢠ⏕≀㉁࡜ࡋ࡚, Ampicillin (ABPC㸹ᐩኈࣇ࢖ࡅࢀࡤ, 2050 ᖺ࡟ࡣୡ⏺࡛, ᖺ㛫⣙ 1000 ୓ேࡀ࣒ࣝ࿴ග⣧⸆(ᰴ)〇), Fradiomycin sulfate (FRM㸹

2).

ࡇࢀࡽ

ᮾி໬ᡂᕤᴗ(ᰴ)〇), Polymyxin B sulfate (PL-B㸹

ࡢឤᰁ⑕࡟ࡣ, ᢠ⏕≀㉁ࢆ⏝࠸ࡓ἞⒪ࡀ⾜ࢃࢀᐩኈࣇ࢖࣒ࣝ࿴ග⣧⸆(ᰴ)〇), Amphotericin Bࡿࡀ, ᢠ⏕≀㉁ࡣᵝࠎ࡞๪స⏝ࢆᘬࡁ㉳ࡇࡍࡇ

(AMPH-B 㸹 ᐩ ኈ ࣇ ࢖ ࣝ ࣒ ࿴ ග ⣧ ⸆ ( ᰴ ) 〇 ),࡜ࡀ▱ࡽࢀ࡚࠸ࡿ 3, 4).

≉࡟, ┿⳦ឤᰁ⑕࡟࠾࠸

Itraconazole (ITCZ 㸹ᮾி໬ᡂᕤ ᴗ (ᰴ )〇 ), 5-5)ࡢ἞⒪࡟୰

Fluorocytosine (5-FC㸹ᮾி໬ᡂᕤᴗ(ᰴ)〇)ࢆ౑

ᚰⓗ࡞ᙺ๭ࢆᯝࡓࡋ࡚࠸ࡿ Amphotericin B ࡣ,

⏝ࡋࡓ. ే⏝≀㉁࡜ࡋ࡚㑅ᐃࡋࡓኳ↛⣔ᢠ⳦๣ᢠ┿⳦ࢫ࣌ࢡࢺࣝࡣᗈ࠸ࡶࡢࡢ, ⭈ẘᛶࡀᙉࡃ,࡟ ࡣ , Fig. 1 ࡟ ♧ ࡋ ࡓ Benzyl isothiocyanateప࣒࢝ࣜ࢘⾑⑕➼ࢆᘬࡁ㉳ࡇࡍࡇ࡜ࡀ▱ࡽࢀ

(BITC㸹ᮾி໬ᡂᕤᴗ(ᰴ)〇), Cinnamaldehyde࡚࠸ࡿ 6, 7). ࡲࡓ, ᪂つᢠ⏕≀㉁ࡢ㛤Ⓨࡣ, ᪂つ(CAL㸹ᐩኈࣇ࢖࣒ࣝ࿴ග⣧⸆(ᰴ)〇)ࢆ⏝࠸ࡓ.

ࢱ࣮ࢤࢵࢺศᏊࡢ᥈⣴ࡀᅔ㞴࡛࠶ࡾ, 㛤Ⓨࢥࢫ౪ヨ⳦ᰴ࡟ࡣ, Escherichia coli JM109 (E. coliࢺ, ཰┈ᛶ࡜࠸ࡗࡓほⅬ࠿ࡽ, ೵⁫ഴྥ࡟࠶ࡿ

JM109㸹ࢱ࢝ࣛࣂ࢖࢜(ᰴ)), ࢝ࣥࢪࢲᒓࡢ୰࠿

8).

ࡽ ,

Ṛஸࡍࡿ࡜ࡢヨ⟬ࡀⓎ⾲ࡉࢀ࡚࠸ࡿ

࡚, 㧗㢖ᗘ࡛Ⓨ⏕ࡍࡿ࢝ࣥࢪࢲ⑕

ࡑࡢࡓࡵ, ᪤Ꮡࡢᢠ⏕≀㉁ࢆ౑⏝ࡋ࡚, ๪స

Candidaparapsilosis

NBRC1396(C.

parapsilosis NBRC1396㸹⊂❧⾜ᨻἲே〇ရホ౯⏝ࢆ㍍ῶࡉࡏࡿ἞⒪ἲࡀᚲせ࡛࠶ࡿ.
ᮏ◊✲࡛ࡣ, ᢠ⏕≀㉁࡜, ᢠ⏕≀㉁࡜ࡣ␗࡞

ᢏ⾡ᇶ┙ᶵᵓ)ࢆ㑅ᐃࡋ, ᮏ◊✲࡛ࡢヨ㦂ᑐ㇟ࡿస⏝ᶵᵓࢆ᭷ࡍࡿኳ↛⣔ᢠ⳦๣࡛࠶ࡿ

࡜ࡋࡓ. ྾ගᗘࡢ ᐃ࡟౑⏝ࡋࡓศගගᗘィࡣ,

Benzyl(ᰴ)᪥❧〇సᡤ〇 Spectrophotometer UV-Vis U-

isothiocyanate(BITC),࠾ ࡼ ࡧCinnamaldehyde (CAL)࡜ࡢ୕๣ే⏝࡟ࡼࡿᢠ⏕3310 ࡟࡚ ᐃࡋࡓ.

≀㉁ࡢ౑⏝㔞๐ῶࢆヨࡳࡓ. WHO ࡀ᪂つᢠ⳦⸆࡜ࡋ࡚⥭ᛴ࡟ᚲせ࡞⣽⳦࡟㑅ᐃࡋࡓࡶࡢࡢ
୰࠿ࡽ 9), ⭠ෆ⣽⳦࡛࠶ࡿ E. coli, ࡲࡓ, Candida
spp.ࡢ୰࡛ࡶ, Candida albicans ࡟ḟࡄṚஸ⋡ࢆ♧ࡍ Candida parapsilosis10)࡟ᑐࡍࡿຠᯝࢆㄪ
Fig. 1 Chemical structures of BITC (left)
and CAL (right)

࡭ࡓ. ࡇࢀࡲ࡛࡟, ஧๣ే⏝ຠᯝࢆ♧ࡍሗ࿌ࡣከࡃ࡞ࡉࢀ࡚࠸ࡿ 6, 11, 12)ࡀ, ஧๣ࡢే⏝࡛ࡣᢠ⏕≀㉁ࡢ౑⏝㔞๐ῶ࡟ࡣ㝈ᗘࡀ࠶ࡿ. ࡑࡢࡓࡵ,୕๣ే⏝࡟ࡼࡾ, ࡉࡽ࡞ࡿᢠ⏕≀㉁ࡢ౑⏝㔞๐

2.2 ᭱ᑠⓎ⫱㜼Ṇ⃰ᗘ ᐃヨ㦂ᮏ◊✲ࡢᢠ⳦ᛶホ౯ࡣ&OLQLFDODQG/DERUDWRU\ῶࢆ᳨ウࡋࡓ.
ࡉࡽ࡟, ⸆๣ࡢ๪స⏝ࢆ㍍ῶࡉࡏࡿ࢔ࣉ࣮ࣟ

6WDQGDUGV ,QVWLWXWH &/6, ࠾ࡼࡧ᪥ᮏ໬Ꮫ⒪ἲᏛ

ࢳ࡜ࡋ࡚, ⸆๣ࡢ࣐࢖ࢡࣟ࢝ࣉࢭࣝ (MC)໬ࢆ

఍ࡢヨ㦂ἲࢆཧ⪃࡟ ే⏝ヨ㦂ࡢࣉࣟࢺࢥࣝࢆ

᳨ウࡋࡓ. MC ໬࡟ࡼࡾ, ⸆๣ࡀᚎᨺ⬟ࢆ♧ࡍ⪃᱌ࡋ ᚤ㔞ᾮయᕼ㔘ἲ࡟ࡼࡾ ᭱ᑠⓎ⫱㜼Ṇࡇ࡜ࡀ▱ࡽࢀ࡚࠾ࡾ, ࡲࡓ, ᮦᩱ, ⃰ᗘ, 〇ἲ⃰ᗘ 0LQLPXPLQKLELWRU\FRQFHQWUDWLRQ㸸0,& ࢆ
22ᐃࡋࡓ  

5 µL ᥋✀ࡋ, ᜏ ᵴ࡟࡚ 35Υ, 24 h ࡢ᮲௳࡛ᇵ
㣴ᚋ, ྛ࢙࢘ࣝ୰ࡢ࡞࠿࡛, ⳦ࡢⓎ⫱ࡀㄆࡵࡽ

2.2.1 ᇵᆅE. coli JM109 ࡛ࡣ, ⁛⳦῭ࡳ Mueller-Hintonࢀ࡞࠸᭱ᑠ⃰ᗘࢆ MIC ࡜ࡋࡓ.

Broth ࡟, ࢁ㐣⁛⳦ࡋࡓ 25 mg/L ࡢ Mg2+࠾ࡼࡧ

2.4 ే⏝ຠᯝࡢุᐃ
ే⏝ຠᯝࢆ ᐃࡋࡓ MIC ್ࢆ⏝࠸࡚, ే⏝ຠᯝࡢ᭷↓ࢆุᐃࡍࡿ FIC index ࢆ⟬ฟࡋࡓ 15).
ୗグ࡟♧ࡍィ⟬ᘧ࡟ࡼࡾ, ⟬ฟ್ࡀӌ0.5 ࢆ┦஌స⏝, 0.5㸺 ~ ӌ1.0 ࢆ┦ຍస⏝, 1.0㸺 ~ ӌ
2.0 ࢆ୙㛵, 㸼2.0 ࢆᣕᢠస⏝࡜ࡋࡓ. ࡞࠾, ┦஌స⏝࠾ࡼࡧ┦ຍస⏝࡜࡞ࡿ FIC index 1.0 ௨ୗࢆ♧ࡋࡓࡶࡢࢆే⏝ຠᯝ᭷ࡾ࡜ࡋࡓ.

2+

50 mg/L ࡢ Ca ࢆῧຍࡋ, 2 mol/L ࡢ NaOH Ỉ⁐ᾮ࡛ pH 7.3 ࡟ㄪᩚࡋࡓ Cation-adjusted MuellerHinton Broth (CAMHB)ᇵᆅࢆ౑⏝ࡋࡓ.
C. parapsilosis NBRC1396 ࡟ ᑐ ࡋ ࡚ ࡣ , Lgulutamine ῧຍ RPMI 1640 ᇵᆅ (ࢼ࢝ࣛ࢖ࢸࢫࢡ ( ᰴ ) 〇 ) ࡟ , 3-(N-morpholino) propanesulfonicacid (MOPS㸹ࢼ࢝ࣛ࢖ࢸࢫࢡ(ᰴ)〇)ࢆ 0.165

2.5 ࣐࢖ࢡࣟ࢝ࣉࢭࣝࡢస〇
Table 1 ࡢ⤌ᡂ࡟ᚑ࠸, ⸆๣ῧຍ 1.0 wt % ࢔ࣝࢠࣥ㓟ࢼࢺ࣒ࣜ࢘Ỉ⁐ᾮ (Na-Alg㸹ࢼ࢝ࣛ࢖ࢸࢫࢡ(ᰴ)〇)ࢆࢩࣜࣥࢪ࡟࠸ࢀ, ὀᑕ㔪 (ෆᚄ0.70 mm; ࢸࣝࣔ(ᰴ))ࢆ㏻ࡌ࡚, ᾮ㠃࠿ࡽࡢ㧗ࡉ 5 cm ࡼࡾ, ࢫࢱ࣮࣮ࣛ᧠ᢾୗ࡟࠶ࡿ 1.0 wt %ࡲࡓࡣ 5.0 wt % ሷ໬࢝ࣝࢩ࣒࢘(ᐩኈࣇ࢖࣒ࣝ
࿴ග⣧⸆(ᰴ)〇)Ỉ⁐ᾮ୰࡬⁲ୗࡉࡏ, ࢔ࣝࢠࣥ㓟࢝ࣝࢩ࣒࢘ (Ca-Alg) ࢤࣝ࡜ࡍࡿ⢏ᚄ⣙ 2mm ࡢྛ⸆๣ࢆῧຍࡋࡓ MC ࢆࡑࢀࡒࢀస〇ࡋ
ࡓ. MC ᙧᡂᚋ, ࢞ࣛࢫࣇ࢕ࣝࢱ࣮ࢆ⏝࠸࡚, ሷ໬࢝ࣝࢩ࣒࢘࡜ MC ࢆศ㞳ࡋ, MC ࢆᅇ཰ࡋࡓ.

mol/L ࡜࡞ࡿࡼ࠺࡟ຍ࠼ࡓࡶࡢࢆ, 2 mol/L ࡢ
NaOH Ỉ⁐ᾮ࡛ pH 7.0 ࡟ㄪᩚࡋ, ࢁ㐣⁛⳦ࡋࡓࡶࡢࢆ⏝࠸ࡓ.

2.2.2 ᥋✀⸆๣ࡢㄪ〇⸆๣ࡢຊ౯ࡶ⪃៖ࡋࡓ࠺࠼࡛⛗㔞ࡋ, Ỉ⁐ᛶࡢᢠ⏕≀㉁ࡣ⵨␃Ỉ, 㠀Ỉ⁐ᛶࡢᢠ⏕≀㉁࠾ࡼ
ࡧ BITC, CAL ࡣ DMSO (ᐩኈࣇ࢖࣒ࣝ࿴ග⣧⸆(ᰴ)〇)ࢆ, ࡑࢀࡒࢀ┠ⓗ⃰ᗘ࡜࡞ࡿࡼ࠺࡟ຍ࠼,࣎ࣝࢸࢵࢡࢫ࣑࢟ࢧ࣮ࢆ⏝࠸࡚᧠ᢾ, ⁐ゎࡉࡏࡓᚋ, ࢁ㐣⁛⳦ࡋࡓࡶࡢࢆ౑⏝ࡋࡓ. ࡑࡢᚋ,

Table 1 Composition of antimicrobial agents
encapsulated Na-Alg aq

ㄪ〇ࡋࡓᇵᆅࢆ⏝࠸࡚, ⸆๣ࡢ 2 ಸᕼ㔘⣔ิࢆస〇ࡋ, VIORAMO 〇୸ᗏ 96 well plate ࡢྛ࢘$PRXQW J

࢙ࣝ࡟, ඲㔞ࡀ 100 µL ࡜࡞ࡿࡼ࠺࡟᥋✀ࡋࡓ.
࡞࠾, ᮏᐇ㦂ࡢ⃰ᗘ࡛ࡣ, ⸆๣ࢆΰྜࡋࡓ㝿ࡢ⸆๣ࡢᯒฟࡣ☜ㄆࡉࢀ࡞࠿ࡗࡓ.

2.2.3 ᥋✀⳦ᾮࡢㄪ〇࠾ࡼࡧᇵ㣴᫬㛫
E. coli JM109 ࡣࢽ࣮ࣗࢺ࢚ࣜࣥࢺᐮኳᇵᆅ, C.
parapsilosis NBRC1396 ࡣ, ࣏ࢸࢺࢹ࢟ࢫࢺ࣮ࣟ

$03+%)&

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'LVWLOOHGZDWHUࢫᐮኳᇵᆅୖ࡟࠾࠸࡚, 35Υ, 24 h ࡢ᮲௳࡛๓ᇵ
㣴ࡋࡓ. ๓ᇵ㣴ࡋࡓ⳦యࢆ 0. 85 %⏕⌮㣗ሷỈ୰࡟ᠱ⃮ࡉࡏ, ࣎ࣝࢸࢵࢡࢫ࣑࢟ࢧ࣮ࢆ⏝࠸࡚⳦యࢆ⏕⌮㣗ሷỈ୰࡟ศᩓࡉࡏࡓ. ࡑࡢᚋ, ᠱ⃮ᾮࢆ McFarland 0.5 ࡟ㄪᩚᚋ, ᭱⤊᥋✀⳦ᩘࡀ E.
coli JM109㸸⣙ 5.0×104 cells/well, C. parapsilosis
NBRC1396㸸⣙ 5×10 ~ 2.5×102 cells/well ࡜࡞ࡿ)LJ   3KRWRJUDSK RI WKH SUHSDUHG
PLFURFDSVXOHVࡼ࠺࡟ㄪᩚࡋࡓ. ࡑࡢᚋ, 96 well plate ࡟⳦ᾮࢆ23

್ࡣ, ༢๣ : 0.125 µg/mL ࠿ࡽ, BITC ࡜ࡢ஧๣ే⏝ : 0.0625 µg/mL, BITC ࡜ CAL ࡜ࡢ୕๣ే
⏝ : 0.0313 µg/mL ࡬࡜పୗࡋ, PL-B ࡢ౑⏝㔞ࡣ༢๣࡜ẚ㍑ࡍࡿ࡜, ୕๣ే⏝࡛ࡣ 1/4 ࡟ᢚ࠼ࡽࢀࡓ. ࡲࡓ, Fig. 3 ࡟♧ࡋࡓ FIC index ࡢ⟬ฟ⤖ᯝࡼࡾ, BITC ࡜ࡢ஧๣ే⏝, BITC ࡜ CAL ࡜ࡢ୕๣ే⏝࡛ࡣ, ┦ຍຠᯝ㸦FIC indexӌ1.0㸧ࢆ♧ࡍࡇ࡜ࡀศ࠿ࡗࡓ. PL-B ࡣ, ࢢ࣒ࣛ㝜ᛶ⳦ࡢ⣽⳦⣽⬊⭷࡟స⏝ࡍࡿ⸆๣࡛࠶ࡿ 16). ࡲࡓ, BITC࠾ࡼࡧ CAL ࡢヲ⣽࡞ᢠ⳦࣓࢝ࢽࢬ࣒ࡣ᫂ࡽ࠿
࡜ࡉࢀ࡚࠸࡞࠸ࡀ, ࢖ࢯࢳ࢜ࢩ࢔ࢿ࣮ࢺ(ITC)㢮ࡣ, ⣽⳦ࡢ㠀≉␗ⓗ࡞㓝⣲ࡢ୙ά໬, ࿧྾⣔ࡢస⏝㞀ᐖ࡟ຍ࠼, ࢱࣥࣃࢡ㉁ࡢᨵኚ࡟ࡼࡿ⣽⳦⣽⬊⭷ᦆയ➼ࡀሗ࿌ࡉࢀ࡚࠸ࡿ 17, 18). ࡲࡓ,
ITC 㢮ࡢᢠ⳦࣓࢝ࢽࢬ࣒ࡣ, ȕ-ࣛࢡࢱ࣒⣔ᢠ⏕≀㉁ࡼࡾ࣌ࣉࢳࢻ⣔ᢠ⏕≀㉁࡛࠶ࡿ PL-B ࡞࡝࡟㢮ఝࡋ࡚࠸ࡿ࡜ࡢሗ࿌ࡶ࠶ࡿ 19). CAL ࡢᢠ⳦࣓࢝ࢽࢬ࣒ࡣ, ୺࡟⣽⳦⣽⬊ቨ࡟ᦆയࢆ୚࠼ࡿ
20)ࡓࡵ, BITC, CAL ࡀ E. coli ࡢእ⭷ᵓ㐀➼࡟ᦆയࢆ୚࠼ࡓࡇ࡜࡟ࡼࡾ, ⣽⳦⣽⬊⭷࡟స⏝Ⅼࢆࡶࡘ PL-B ࡀస⏝ࡋࡸࡍࡃ࡞ࡗࡓࡇ࡜ࡀせᅉ࡜࡞ࡾ, ┦ຍຠᯝࢆ♧ࡋࡓ࡜⪃࠼ࡽࢀࡿ.
ABPC, FRM ࡣ஧๣ే⏝, ୕๣ే⏝࡜ࡶ࡟ే⏝ຠᯝࡣ☜ㄆࡉࢀ࡞࠿ࡗࡓ. ABPC ࡣ⣽⬊ቨ࣌ࣉࢳࢻࢢࣜ࢝ࣥ࡟స⏝ࡍࡿࡀ, BITC ࡜స⏝࣓࢝ࢽࢬ࣒ࡀ␗࡞ࡿ 19)ࡓࡵ, ే⏝ຠᯝࢆ♧ࡉ࡞࠿ࡗࡓ࡜⪃࠼ࡽࢀࡿ. ࡲࡓ, FRM ࡞࡝ࡢ࢔࣑ࣀࢢ
ࣜࢥࢩࢻ⣔ᢠ⏕≀㉁ࡣ, ࢝ࢳ࢜ࣥᛶࡢ࢔࣑ࣀᇶ
ࢆከᩘ᭷ࡍࡿᵓ㐀࡛࠶ࡿ. ࡑࡢࡓࡵ, ⣽⳦እ⭷ࡢ࣏ࣜከ⢾ࡢ Mg2+ࢆ᤼㝖ࡍࡿࡇ࡜࡛, ⮬ࡽ࣏ࣜከ⢾࡜⤖ྜࡋ, እ⭷ᵓ㐀ࢆ◚ቯࡍࡿࡇ࡜࡛,ࣜ࣎ࢯ࣮࣒࡟฿㐩ࡋ࡚స⏝ࡍࡿࡇ࡜࡛ᢠ⳦ᛶ
ࢆ♧ࡍ 21). ࡋ࠿ࡋ, FRM ༢๣࡛ࡢ MIC ್௨ୗ

2.6 ࣐࢖ࢡࣟ࢝ࣉࢭࣝࡢ⸆๣⁐ฟヨ㦂ෆໟࡍࡿ⸆๣ࡢ≉ᚩⓗ࡞྾཰Ἴ㛗, ࡍ࡞ࢃࡕAMPH-B, ITCZ, 5-FC (385, 262, 234 nm), BITC,CAL (243, 292 nm)࡟࠾ࡅࡿ྾ගᗘࢆ, ࢔ࢭࢺࢽࢺࣜࣝ(MeCN㸹ᐩኈࣇ࢖࣒ࣝ࿴ග⣧⸆(ᰴ)〇))0.9%⏕⌮㣗ሷỈ (Saline) [6:4, v/v]࡟⁐ゎࡉࡏ࡚ᐃࡋ, ᳨㔞⥺ࢆసᡂࡋࡓ. స〇ࡋࡓ⸆๣ෆໟ
MC 1.0 g ࢆ, 50 mM ࡢࢡ࢚ࣥ㓟୕ࢼࢺ࣒ࣜ࢘(ᐩኈࣇ࢖࣒ࣝ࿴ග⣧⸆(ᰴ)〇)ࢆ⁐ゎࡉࡏࡓ 0.9 %⏕⌮㣗ሷỈ 50 mL ୰࡟ᾋ㐟࣭ᠱ⃮ࡉࡏ, 37Υ, 140
rpm, 30 min ࡢ᮲௳࡛᣺࡜࠺ࡋ, MC ࢆ᏶඲࡟⁐ゎࡉࡏࡓ. ࡇࡢ⁐ᾮࢆࢩࣜࣥࢪࣇ࢕ࣝࢱ࣮࡛ࢁ㐣ࡋࡓᚋ, ྾ගᗘࢆ ᐃࡋ, ᳨㔞⥺ࡢ್࠿ࡽMC ࡢ⸆๣ෆໟ㔞ࢆ⟬ฟࡋࡓ.
⁐ฟヨ㦂ࡣ, ⏕⌮㣗ሷỈ 50 mL ࢆຍ࠼ࡓ 100
mL ࢚࣐ࣝࣞࣥ࢖࣮ࣖࣇࣛࢫࢥ࡟, MC 1.0 g ࢆᾋ㐟ࡉࡏ, 37Υ, 100 rpm ࡢ᮲௳࡛᣺࡜࠺ࡋࡓ. 1
h ẖ࡟⁐ฟᾮࡢࡳࢆ 500 µL ศྲྀࡋ, ࡑࡢ㒔ᗘ,᪂ࡋ࠸⏕⌮㣗ሷỈࢆ 500 µL ຍ࠼ࡿ᧯సࢆ, ᣺࡜࠺㛤ጞ࠿ࡽ 6 h ⤒㐣ࡲ࡛, ⤒᫬ⓗ࡟⧞ࡾ㏉ࡋ
ࡓ. ศྲྀࡋࡓ⁐ฟᾮࢆ MeCN-Saline [6:4, v/v] ࡟⁐ゎࡉࡏ࡚྾ගᗘࢆ ᐃࡋ, ෆໟ㔞ࢆࡶ࡜࡟,MC ࡢ⸆๣⁐ฟ⋡ࢆ⟬ฟࡋࡓ.
3 ᐇ㦂⤖ᯝ࠾ࡼࡧ⪃ᐹ
3.1 ే⏝≀㉁ࡢᢠ⳦ᛶ࠾ࡼࡧᢠ┿⳦ᛶホ౯
7DEOH ࡟%,7&&$/ ༢๣ࡢ (FROL-0 ࠾
ࡼࡧ &SDUDSVLORVLV1%5& ࡟ᑐࡍࡿ 0,&ᐃヨ㦂⤖ᯝࢆ♧ࡍ ౪ヨ⳦ᰴ࡟ᑐࡋ࡚7DEOH ࡢ⃰ᗘ࡛ᢠ⳦ᛶࢆ♧ࡍࡇ࡜ࡀ☜ㄆࡉࢀࡓ ࡲࡓ7DEOH ࡼࡾ &$/ ࡢ & SDUDSVLORVLV ࡟ᑐࡍࡿᢠ
┿⳦ᛶࡣ≉࡟ᙉ࠸ࡇ࡜ࡀ☜ㄆࡉࢀࡓ
7DEOH  0,&IRU%,7&DQG&$/RIPLFURELDOVWUDLQV
0LFURELDOVWUDLQ

0,& JP/
%,7&

&$/

(FROL-0





&SDUDSVLORVLV1%5&



 

A㸸ABPC + BITC B㸸ABPC + CAL C㸸ABPC + BITC + CALD㸸FRM + BITC E㸸FRM + CAL F㸸FRM + BITC + CAL
G㸸PL-B + BITC H㸸PL-B + CAL I㸸PL-B + BITC + CAL

3.2 (FROL-0 ࡟ᑐࡍࡿే⏝ヨ㦂
Table 3 ࡟, E. coli JM109 ࡟ᑐࡍࡿ⸆๣ే⏝ヨ㦂ࡢ⤖ᯝࢆ, Fig. 3 ࡟ే⏝ヨ㦂࡛ࡢ MIC ್ࢆࡶ࡜࡟⟬ฟࡋࡓ FIC index ࡢ⤖ᯝࢆ♧ࡍ. Table 3 ࡼࡾ, PL-B ࡛ࡣ୕๣ే⏝ຠᯝࢆ♧ࡋ, PL-B ࡢ MIC)LJ  ),&LQGLFHVRIFRPELQDWLRQVFRQVLVWLQJ
RIDQWLELRWLFVQDWXUDODQWLPLFURELDODJHQWV
LQ(FROL-0

24

Table 3 MICs for single, double and triple combinations of antibiotics, BITC and CAL against E. coli
JM109

&RPELQDWLRQVRIDQWLEDFWHULDODJHQWV$QWLELRWLFV

%,7&

&$/

GRXEOHWULSOH

$%3&

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7ULSOH$%3&%,7&&$/

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7ULSOH)50%,7&&$/ 

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6LQJOH3/%  

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3/%%,7&   

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3/%&$/  

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3/%%,7&&$/   

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6LQJOH
$%3&

)50

3/%

5DWLRͤ

0,& JP/'RXEOH'RXEOH'RXEOH
7ULSOHͤ Ratio : Ratio of [MIC of antibiotics when Combined use of double or triple] to [MIC of antibiotics alone],that is, ratio of decrease in antibiotics usage.
),&LQGH[ GUXJV  0,&RI$QWLELRWLFVLQFRPELQHG 0,&RI %,7&&$/ LQFRPELQHG
0,&RI$QWLELRWLFVDORQH
0,&RI %,7&&$/ DORQH),&LQGH[ GUXJV  0,&RI$QWLELRWLFVLQFRPELQHG 0,&RI%,7&LQFRPELQHG 0,&RI&$/LQFRPELQHG

0,&RI$QWLELRWLFVDORQH
0,&RI%,7&DORQH
0,&RI&$/DORQH

࡛ࡣ, ࣏ࣜከ⢾ࡢ㐟㞳స⏝ࡀప࠿ࡗࡓࡇ࡜࡛,ే⏝ຠᯝࢆ♧ࡉ࡞࠿ࡗࡓ࡜⪃࠼ࡽࢀࡿ.

⏝≀㉁࡛࠶ࡿ %,7& &$/ ࡢ 0,& ್ࡶ ୕๣ే

3.3 & SDUDSVLORVLV 1%5& ࡟ᑐࡍࡿే⏝ヨ㦂
7DEOH ࡟&SDUDSVLORVLV1%5& ࡟ᑐࡍࡿ୕๣ే⏝ヨ㦂⤖ᯝࢆ)LJ ࡟ ),&LQGH[ ࡢ⟬ฟ⤖ᯝࢆ♧ࡍ 㑅ᐃࡋࡓ඲࡚ࡢᢠ⏕≀㉁ ࡍ࡞ࢃࡕ $03+% ,7&= )& ࡜ &$/ ࡢ஧๣ే⏝࡛ࡣᢠ⏕≀㉁ࡢ 0,& ್ࡀ  a  ࡟ ࡉࡽ࡟%,7& ࢆຍ࠼ࡓ୕๣ే⏝࡛ࡣa ࡟ῶᑡࡋ
ᢠ⏕≀㉁ࡢ౑⏝㔞ࡀ஧๣ే⏝᫬ࡼࡾࡶῶᑡࡍ
ࡿࡇ࡜ࡀศ࠿ࡗࡓ ࡲࡓ)LJ ࡟♧ࡍ ),&LQGH[
ࡢ್ࡼࡾ,7&=)& ࡜ &$/ ࡜ࡢ஧๣ే⏝࡟࠾࠸࡚ࡣ ┦ຍຠᯝᲢ),&LQGH[ӌ ࢆ♧ࡋ ࡉࡽ࡟ ୕๣ే⏝ヨ㦂࡟࠾࠸࡚ࡣ ඲࡚ࡢ⤌ࡳྜࢃࡏ࡛┦ຍຠᯝࢆ♧ࡋ ᢠ⏕≀㉁ࡔࡅ࡛ࡣ࡞ࡃే

& SDUDSVLORVLV ࡟ᑐࡍࡿ %,7& ࠾ࡼࡧ &$/ ࡢ⏝࡟ࡼࡾపࡃ࡞ࡗࡓ
ᢠ⳦࣓࢝ࢽࢬ࣒࡟㛵ࡋ࡚ࡶ ヲࡋࡃࡣゎ᫂ࡉࢀ࡚࠸࡞࠸ࡶࡢࡢ %,7& ࡣ┿⳦⣽⬊ቨ࡟ᦆയࢆ
୚࠼ ௦ㅰ㓝⣲ࢆ㜼ᐖࡍࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿ  ࡲࡓ &$/ࡣ┿⳦⣽⬊ቨ࡬ࡢᦆയ࡟ຍ࠼

⣽⬊ቨྜᡂ㓝⣲࡬ࡢ㜼ᐖ ┿⳦୰ࡢ࣑ࢺࢥࣥࢻ
ࣜ࢔࠾ࡼࡧ┿⳦⣽⬊⭷࡟ᦆയࢆ୚࠼ࡿ࡜࠸ࡗ
ࡓᢠ⳦࣓࢝ࢽࢬ࣒ࡀሗ࿌ࡉࢀ࡚࠸ࡿ a  ࡲࡓᮏ◊✲࡟ࡼࡾ &$/ ࡢ & SDUDSVLORVLV ࡟ᑐࡍࡿ0,& ࡣ ⣽⳦࡛࠶ࡿ ( FROL ࡼࡾࡶప࠸ഴྥ
7DEOH  ࡟࠶ࡿᮏ◊✲࡛ࡣ┿⳦⣽⬊⭷࡟స⏝ࡍࡿ $03+%
,7&= ┿⳦ࡢ᰾㓟ྜᡂࢆ㜼ᐖࡍࡿ )& ࢆ㑅ᐃࡋࡓ   ࡇࢀࡽࡢ⸆๣ࡣ ඲࡚┿⳦⣽⬊ቨෆ㒊
25

Table 4 MICs for single, double and triple combinations of antibiotics, BITC and CAL against C.
parapsilosis NBRC1396&RPELQDWLRQVRIDQWLIXQJDODJHQWV$QWLELRWLFV

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7ULSOHͤ Ratio : See footnote to Table 3.

ᑐࡍࡿᶵ⬟㜼ᐖ➼ࡢከᵝ࡞ᢠ⳦࣓࢝ࢽࢬ࣒࡟
ࡼࡾ & SDUDSVLORVLV ࡟ᦆയࢆ୚࠼ࡓࡇ࡜࡛ ᢠ⏕≀㉁ࡀ┿⳦⣽⬊ቨෆ㒊࡬ࡢྲྀࡾ㎸ࡳࡀಁ㐍ࡉࢀ ஧๣ే⏝᫬ࡼࡾࡶࡉࡽ࡟ 0,& ್ࡀపࡃ࡞ࡗࡓ࡜⪃࠼ࡽࢀࡿ㸬

3.4 ࣐࢖ࢡࣟ࢝ࣉࢭࣝࡢ⸆๣⁐ฟヨ㦂)LJ ࡟⸆๣ῧຍ 0& ࡢ⸆๣⁐ฟᣲືࢆ♧ࡍ
⸆๣ࢆ 0& ໬ࡍࡿࡇ࡜࡛ ⸆๣ࡀᚎᨺ⬟ࢆ᭷ࡍࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡾ ሷ໬࢝ࣝࢩ࣒࢘⃰ᗘࡀ㧗࠸ࡶࡢ࡯࡝ ࡲࡓ ศᏊ㔞ࡀ኱ࡁ࠸ࡶࡢ࡯࡝
⁐ฟ㏿ᗘࡀ㐜࠸ഴྥ࡟࠶ࡗࡓ ᮏ◊✲࡛ࡣ$OJ1D ࡜ሷ໬࢝ࣝࢩ࣒࢘୰ࡢ &Dࡢ࢖࢜ࣥᯫ
ᶫ࡟ࡼࡾ &D$OJ ࢤࣝ࡜࡞ࡿᾮ୰◳໬⿕⭷ἲ࡟
ࡼࡾ 0& ࢆస〇ࡋࡓ&D$OJ ࡣ ౯㔠ᒓሷỈ⁐ᾮ୰࡟࠾࠸࡚&D$OJ ࢤࣝ୰ࡢ &D࡜ ౯㔠ᒓ࢖࢜ࣥ㛫࡛ࡢ࢖࢜ࣥ஺᥮཯ᛂ࡟ࡼࡾ &D$OJ
ࡢ୍㒊ࡀ 1D$OJ ࡜࡞ࡿࡇ࡜࡛ &D$OJ ࡢᯫᶫᐦᗘࡀపୗࡋ 0& ࡢᙎᛶ⋡ࢆᙅࡵࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿ ࡑࡢࡓࡵ ౯㔠ᒓሷỈ⁐ᾮ୰࡛ࡣ
0& ࡢ㧗ศᏊ⥙┠ෆ࡟ከࡃࡢỈࢆྵࢇࡔᵓ㐀య࡜࡞ࡿ   ௨ୖࡢⅬ࠿ࡽ &D$OJ ࡜⁐ฟᾮ㛫࡟

a㸸AMPH-B + BITC b㸸AMPH-B + CAL c㸸AMPH-B + BITC + CAL
d㸸ITCZ + BITC e㸸ITCZ + CAL f㸸ITCZ + BITC + CAL
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DQWLELRWLFV QDWXUDO DQWLPLFURELDO DJHQWV LQ &
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26

Fig. 5 Comparison of elution rates of MC containing various antimicrobial agents at 37Υ in saline
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27

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MATERIAL TECHNOLOGY 材料技術
昭和62年8月5日第三種郵便物許可

令和３年4月25日発行（隔月25日発行）

第39巻

第２号

定価2,100円