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Human RNF4 monoclonal antibody
Category
Catalog No.
Applications
Reactivity

Monoclonal antibody
R-R-001 (clone # 11-3.1)
WB, IF, IP
HumanImmunogen information
Immunogen
Recombinant GST-tagged human full-length RNF4
UniProt ID
P78317
Synonyms
RING finger protein 4, SNURF (Small nuclear ring finger protein)
Gene ID
6047
Product information
SourceClone No.
Isotype
Purification method
Lot No.
Concentration
BufferStorageMouse
11-3.1IgG2b
Protein A purification
001
1.0 mg/mL
50% glycerol/PBS, pH7.4, w/o sodium azide
Store at -20°C.

Recommended dilutions
WB
1:1000 – 1:2000
IF
1:50 – 1:200
IP
1:20 – 1:50
Western Blotting

Validation of this anti-RNF4 mAb
using siRNA
Exponentially growing HeLa cells were transfectedwith control siRNA (lane 1) or siRNA against RNF4(lane 2). After 24 h incubation, cells were lysed
directly in SDS sample buffer, and proteins weresubjected to 10% SDS–PAGE, followed byimmunoblot analysis using mouse anti-RNF4
monoclonal antibody (Catalog #R-R-001, upperpanel) or anti-actin (lower panel) antibody.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

ImmunoprecipitationImmunoprecipitation of HEK293 cells
transiently transfected with FLAG-RNF4
HEK293 cells were transfected with FLAG-tagged
RNF4. RNF4 was immunoprecipitated using antiRNF4 (Catalog #R-R-001) or anti-FLAG m2
monoclonal antibody. Western blot analysis wasperformed on the immunoprecipitated samples with
anti-DYKDDDDK rabbit polyclonal antibody.
Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Immunofluorescence

Detection of endogenous RNF4 in ahumancancer
celllinebyimmunofluorescence
Endogenous RNF4 in a HeLa human cervical carcinoma
cell was detected by immunofluorescence with mouseanti-human RNF4 monoclonal antibody (Catalog # R-R001).

BackgroundA wide variety of proteins that contain a RING domain have been demonstrated to function as
ubiquitin ligases that promote the transfer of ubiquitin from E2s to lysine residues in target
proteins. Among the RING-type E3 ligases, RING finger protein 4 (RNF4 or SNURF) is
proposed to ubiquitylated proteins modified by SUMO and therefore is classified into aparticular category of E3s, termed as the SUMO-targeted ubiquitin ligases (STUbLs). In
mammalian cells, RNF4 is crucial in arsenic therapy for acute promyelocytic leukemia and inresponses against heat shock and hypoxia. RNF4 is also required for the DNA damage
response and base-excision repair (BER)-mediated active DNA demethylation, suggesting that
STUbL activity mediated by RNF4 is important for regulating a wide variety of signalingcascades in vital cellular systems.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

References for human RNF4 monoclonal antibody (R-R-001)
PMID:

27181354

Journal:Biochem Biophys Res Commun.

Application:

IFIF (2021):

3.322

Title:

Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition.

PMID:

24727457

Journal:Biochem Biophys Res Commun.

Application:

WB, IFIF (2021):

3.322

Title:

SUMO-modification and elimination of the active DNA demethylation enzyme TDG incultured human cells.

PMID:

24695317

Journal:Biochem Biophys Res Commun.

Application:

WB

IF (2021):

3.322

Title:The SUMO-targeted ubiquitin ligase RNF4 localizes to etoposide-exposed mitotic
chromosomes: implication for a novel DNA damage response during mitosis.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp