Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Human Interleukin-18 (IL-18) neoepitope monoclonal antibody
Category
Catalog No.
Applications
Reactivity

monoclonal antibody
R-I-002 (clone # 9-10.2)
WB, WES, IF, IP, function-blocking
Human

Immunogen information
Immunogenhuman IL-18 (37-44) peptide
UniProt ID
Q14116
Synonyms
IGIF, IL1F4
Gene ID
3606
Product information
SourceClone No.
Isotype
Epitope
Purification method
Lot No.
Concentration
BufferStorage

Mouse
9-10.2
IgG1
human IL-18 (37-44)
DEAE ion-exchange purification
001
1.0 mg/mL
50% glycerol/PBS, pH7.4, w/o sodium azide
Store at -20°C.

Recommended dilutions
WB
1:1000 – 1:2000
WES
1:250 – 1:300
IF
1:50 – 1:200
IP
1:20 – 1:50
Capillary Western immunoassay (WES)WES analysis of the serum of adult-onset
Still’s disease patients.
IL-18 bands in 6 (43%) of 14 AOSD samples
were clearly visible and had a molecularweight corresponding to IL-1837-193 (Catalog #R-I-002)
WES is a registered trademark of ProteinSimple.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Capillary Western immunoassay (WES)

Characterization of novel mAbs against theneoepitope of human IL-18 cleaved byinflammatory caspase-1/4Capillary Western immunoassay (WES) of serial
dilutions of bacterially purified IL-18 protein
cleaved by caspase-4 (IL-1837-193) (Catalog # PI-001) and Met-IL-1837-193 with the newly
generated neoepitope mAb (Catalog # R-I-002,right panel) and anti-IL-18 mAb (Catalog # R-I001, left panel).
WES is a registered trademark of ProteinSimple.

Immunoprecipitation

Surface Plasmon Resonance analysis

Background
This gene encodes a member of the interleukin-1 (IL-1) family of cytokines. In addition to itsrole in the inflammatory response to microbes, recent studies implicate IL-18 as an importantfactor in human autoimmune, autoinflammatory, allergic, neurological and metabolic diseases.
Similar to IL-1β, pro-IL-18 is processed by inflammatory caspase-1 or caspase-4 to yield
mature, active form (IL-1837-193).
References for human IL-18 neoepitope monoclonal antibody (R-I-002)
PMID:

30615852

Journal:Archives of Biochemistry and Biophysics

Application:

WB, WES, IF, IP, function-blocking

IF (2020):

4.013

Title:

Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal
antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18
and the neoepitope of the inflammatory caspase-cleaved active IL-18

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp