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Recombinant human Interleukin‐18 (IL‐18) (37‐193) protein
Category
Catalog No.

protein and peptide
P‐I‐001

Product information
Product
Recombinant protein of human IL‐1837‐193
SourceE. coliPurification method Bacterially expressed and purified full‐length human IL‐18 was cleaved byUniProt ID
Synonyms
Gene ID

active caspase‐4105‐377. The N‐terminal Tyr of the IL‐18 neoepitope wasconfirmed by Edman degradation sequencing.
Q14116
IGIF, IL1F43606

Lot No.
Concentration
BufferPurityStorage001
1 mg/mL
0.2 μm filtered solution in PBS, pH7.4>95%, by SDS‐PAGE
Store at ‐80°C.

Purification method

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

Purification method.
IL-18 and caspase-4105-377 were expressed in E.
coli, and purified using Ni-NTA agarose. A
mixture of these proteins (IL-18: Caspase-4105-377=10:1) was incubated and then run through a NiNTA agarose column. The flow-through wascollected as IL-1837‐193.
The N-terminal Tyr of IL-1837‐193 was confirmed by
Edman degradation sequencing.

89‐1 Enya‐cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693‐8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Capillary Western immunoassay (WES)

Characterization of novel mAbs against theneoepitope of human IL‐18 cleaved byinflammatory caspase‐1/4
Capillary Western immunoassay (WES) ofserial dilutions of bacterially purified IL-18
protein cleaved by caspase-4 (IL-1837-193)(Catalog # P-I-001) and Met-IL-1837-193 with the
newly generated neoepitope mAb (Catalog #R-I-002, right panel) and anti-IL-18 mAb(Catalog # R-I-001, left panel).
WES is a registered trademark of ProteinSimple.

Capillary Western immunoassay (WES)

Capillary Western immunoassay (WES)

Background
This gene encodes a member of the interleukin-1 (IL-1) family of cytokines. In addition to itsrole in the inflammatory response to microbes, recent studies implicate IL-18 as an importantfactor in human autoimmune, autoinflammatory, allergic, neurological and metabolic diseases.
Similar to IL-1β, pro-IL-18 is processed by inflammatory caspase-1 or caspase-4 to yield
mature, active form (IL-1837-193).
References for recombinant human IL‐1837‐193 protein (P‐I‐001)
PMID:

Title:

30615852

Journal:Archives of Biochemistry and Biophysics

IF (2020):

4.013

Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal
antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18 andthe neoepitope of the inflammatory caspase-cleaved active IL-18

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89‐1 Enya‐cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693‐8501, Japan
https://mabprotein.co.jp