Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Anti-DLVPR (G196) epitope-tag monoclonal antibody
Category
Catalog No.
Applications
Product information
SourceClone No.
Epitope
Isotype
Purification method
Lot No.
Concentration
BufferStoragetag antibody
R-G-001
WB, IP, ChIP, IF

Mouse
G196
Five amino acid sequence Asp-Leu-Val-Pro-Arg (DLVPR)
IgG1
DEAE ion-exchange purification
001
1.0 mg/mL
50% glycerol/PBS, pH7.4, with 0.05% ProClin 300
Store at -20°C.

Recommended dilutions
WB
1:2000 – 1:10000
IP
1:200 – 1:500
ChIP
1:200 – 1:500
IF
1:200 – 1:500
Western Blot

Western blot analysis of FLAG-HA-GFP (FHG)
tagged with C-terminal G196-tag in HeLa cells
using mAb G196 (Catalog # R-G-001).
HeLa cells were transfected with FHG/pcDNA3 or FHGG196/pcDNA3. The cells were lysed and subjected toWestern blotting (WB) with anti-FLAG (M2) or G196 mAbs.
Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

Immunoprecipitation

Permutation ELISA

Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Immunofluorescence

Alexa Fluor is a registered trademark of Life Technologies Corporation.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

ImmunofluorescenceBackgroundmAb G196/G196-epitope peptide (five amino acid sequence Asp-Leu-Val-Pro-Arg, DLVPR) is a new
peptide tagging system for cell biology and biochemistry research. The recognition specificity of
monoclonal antibodies (mAbs) has made mAbs among the most frequently used tools in both basic
science research and in clinical diagnosis and therapies. Precise determination of the epitope allows
the development of epitope tag systems to be used with recombinant proteins for various purposes. A
new family of tag was derived from the epitope recognized by a highly specific mAb G196. The minimal
epitope was the five amino acid sequence Asp-Leu-Val-Pro-Arg. Isothermal titration calorimetry
revealed the high affinity (Kd = 1.25 nM) of the mAb G196/G196-epitope peptide interaction, and G196tag was used to detect several recombinant cytosolic and nuclear proteins in human and yeast cells.

References for G196 monoclonal antibody (R-G-001)
PMID:

28266535

Journal:Scientific Reports

Application:

WB, IF, IP, ChIP

IF (2020):

4.380

Title:

G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small,soluble peptide DLVPR with high affinity.

PMID:

30615852

Journal:Arch Biochem Biophys

Application:

WB, IP, IFIF (2020):

4.013

Title:

Generation and characterization of antagonistic anti-human interleukin (IL)-18
monoclonal antibodies with high affinity: Two types of monoclonal antibodies against
full-length IL-18 and the neoepitope of inflammatory caspase-cleaved active IL-18.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp

Driving Innovation in Antibody Development
The company, mAbProtein,
has been established to help patients, doctors, and researchers.

PMID:

29866182

Journal:Epigenetics Chromatin

Application:

IP

IF (2020):

4.185

Title:

RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces
pombe requires heat-shock molecular chaperones Hsp90 and Mas5.

PMID:

29507312

Journal:Scientific Reports

Application:

WB, IP

IF (2020):

4.380

Title:

Analysis of the oligomeric states of nucleophosmin using size exclusionchromatography.

PMID:

24794433

Journal:Cell Reports

Application:

IFIF (2020):

9.423

Title:

TRIM27/MRTF-B-dependent integrin β1 expression defines leading cells in cancer
cell collectives.

PMID:

27647735

Journal:Genes to Cells

Application:

WB

IF (2020):

1.891

Title:

Four domains of Ada1 form a heterochromatin boundary through different
mechanisms.

PMID:

24307402

Journal:The Journal of Biochemistry

Application:

WB

IF (2020):

3.387

Title:The N-terminus and Tudor domains of Sgf29 are important for its heterochromatinboundary formation function.

PMID:

23819448

Journal:Genes to Cells

Application:

WB

IF (2020):

1.891

Title:

C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in thelarger complex and for heterochromatin boundary function.

mAbProtein Co., Ltd
This product is intended for research purposes only.
Not for therapeutic or diagnostic purposes in humans or animals.
For more information, email info@mabprotein.co.jp.

89-1 Enya-cho, Shimane Univ. Schl. of Med.
Basic Science Bldg., Izumo, Shimane,693-8501, Japan
https://mabprotein.co.jp