キウイフルーツかいよう病菌（Pseudomonas syringae pv. actinidiae (Psa)）に汚染された花粉からのPsaの検出方法の開発および福岡県内で採取された花粉の汚染状況調査

5࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓⳦㸦3VHXGRPRQDVV\ULQJDH SYDFWLQLGLDH 3VD 㸧࡟ởᰁࡉࢀࡓⰼ⢊࠿ࡽࡢ 3VD ࡢ᳨ฟ᪉ἲࡢ㛤Ⓨ࠾ࡼࡧ⚟ᒸ┴ෆ࡛᥇ྲྀࡉࢀࡓ
ⰼ⢊ࡢởᰁ≧ἣㄪᰝ
⳥ཎ㈼ḟ㸨
⚟ᒸ┴࡛ࡣ  ᖺ௨㝆㸪 3VHXGRQRQDVV\ULQJDH SY DFWLQLGLDH ELRYDU㸦3VD㸧࡟ࡼࡿ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺
⑓ࡢ⿕ᐖࡀᣑ኱ࡋ࡚࠸ࡿࠋ3VD ࡟ởᰁࡉࢀࡓⰼ⢊ࡣឤᰁࢆᣑ኱ࡉࡏࡿྍ⬟ᛶࡀ࠶ࡿࡓࡵ㸪ேᕤᤵ⢊࡟ࡣ೺඲ⰼ⢊ࢆ౑⏝ࡋ࡞ࡅࢀࡤ࡞ࡽ࡞࠸ࠋởᰁⰼ⢊࠿ࡽࡢ 3VD ࡢ᳨ฟ᪉ἲࡣࠕ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓ 3VD ⣔⤫ࡢ㜵㝖ᑐ⟇࣐ࢽࣗ࢔ࣝࠖ࡟グ㍕ࡉࢀ࡚࠸ࡿࡀ㸪 ࢧࣥࣉࣝࡈ࡜࡟㧗౯࡞ᑓ⏝ᶵჾ࡛㞟⳦ࡋ࡚࠿ࡽ 3&5 ἲ᳨࡛ฟࡍࡿࡓࡵ㸪᫬㛫࡜ປຊࡀ࠿
࠿ࡾ㸪኱㔞᳨ᐃ࡟ࡣ୙ྥࡁ࡛࠶ࡿࠋ୍᪉㸪ⰼ⢊࠿ࡽ┤᥋ '1$ ࢆᢳฟࡋ㸪3&5 ἲ᳨࡛ฟࡍࡿ᪉ἲࡀሗ࿌ࡉࢀ࡚࠸ࡿࡀ㸪᳨ฟ㝈⏺ࡣ᫂ࡽ࠿࡛ࡣ࡞࠸ࠋᮏ◊✲ࡣⰼ⢊࠿ࡽ┤᥋ '1$ ࢆᢳฟࡋ㸪㜵㝖࣐ࢽࣗ࢔ࣝ࡟グ㍕ࡉࢀࡓࣉࣛ࢖࣐࣮ࢆ⏝࠸ࡓ 3&5ἲ࡟ࡼࡾ㸪3VD ࡀFIXJ ྵࡲࢀࡿேᕤởᰁⰼ⢊࠿ࡽ᳨ฟࡀྍ⬟࡛࠶ࡗࡓࠋḟ࡟㸪㛤Ⓨᡭἲࢆ୰ᚰ࡜ࡋ࡚⏝࠸㸪 ᖺ࡟⚟ᒸ┴ࡢ  ᅡሙ࡛⏕⏘ࡉࢀࡓⰼ⢊ࡢởᰁ≧ἣࢆㄪᰝࡋࡓ⤖ᯝ㸪ᮏ⑓Ⓨ⏕ᅡሙࡢ㞝ᮌ࠿ࡽ᥇ྲྀࡉࢀࡓࡍ࡭࡚ࡢ
ⰼ⢊࠿ࡽ 3VD ࡀ᳨ฟࡉࢀࡓࠋࡲࡓ㸪Ⓨ⏕ᅡሙ࡟㞄᥋ࡋࡓᮍⓎ⏕ᅡሙࡢ㞝ᮌ࠿ࡽ᥇ྲྀࡋࡓⰼ⢊࠿ࡽࡶ 3VD ࡀ᳨ฟࡉࢀ
ࡓࠋ୍᪉㸪↓Ⓨ⏕ᆅ༊࡛⏕⏘ࡉࢀࡓⰼ⢊࠿ࡽ 3VD ࡣ᳨ฟࡉࢀ࡞࠿ࡗࡓࠋࡇࡢࡇ࡜࠿ࡽ㸪Ⓨ⏕ᆅ༊࡛ࡣ೺඲ⰼ⢊ࡢ⏕⏘ࡣᅔ㞴࡛࠶ࡿࡇ࡜ࡀ♧၀ࡉࢀ㸪ᤵ⢊࡟౑⏝ࡍࡿⰼ⢊ࡣᑡ࡞ࡃ࡜ࡶ㸪↓Ⓨ⏕ᆅ༊࡛ࡢ⏕⏘ࡀᚲせ࡜⪃࠼ࡽࢀࡓࠋ
[࣮࣮࢟࣡ࢻ㸸࢟࢘࢖ࣇ࣮ࣝࢶ㸪ởᰁⰼ⢊㸪3&5 ἲ㸪3VHXGRQRQDVV\ULQJDHSYDFWLQLGLDH ELRYDU@
Detection Method of Pseudomonas syringae pv. actinidiae from Contaminated Pollen and Investigation into the Contaminationof Pollen from Male Kiwifruit Vine in Fukuoka Prefecture. KIKUHARA Kenji (Fukuoka Agriculture and Forestry ResearchCenter, Chikushino, Fukuoka 818-8549, Japan) Bull. Fukuoka Agric. For. Res. Cent. 5:5-10 (2019)
In the Fukuoka Prefecture, an outbreak of bacterial canker in kiwifruit, caused by Pseudomonas syringae pv. actinidiae biovar
3 (Psa3), has been occurring since 2014. Psa3-free pollen must be used for artificial pollination because pollen contaminated with
Psa3 may spread this disease. The existing method for Psa3 detection needs expensive and specialist equipment; thus, many
samples cannot be easily analyzed. In addition to this, it was reported that PCR analysis using DNA directly extracted frompollen could detect Psa3, although the degree of sensitivity needed to detect Psa3 was unclear. In this study, Psa3 was detectedfrom artificially contaminated pollen, adjusted to 3×10 4cfu/g, by PCR analysis using DNA directly extracted from pollen. We
investigated contamination of pollen in samples collected from male kiwifruit vines in 36 orchards in the Fukuoka Prefecture in2016. Psa3 was detected in pollen collected from diseased orchards, as well as in pollen collected from non -diseased orchards inareas where the disease occurred. Unsurprisingly, Psa3 was not detected in pollen collected from orchards in areas where thedisease did not occur. The results demonstrated that Psa3-free pollen should be produced in areas where the disease does notoccur.
[Key word: contaminated pollen, detection by PCR, kiwifruit, Pseudononas syringae pv. actinidiae biovar 3]⥴ ゝ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓ࡣ㸪࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ
࠺⑓⳦ 3VHXGRPRQDVV\ULQJDHSYDFWLQLGHDH㸦௨ୗ㸪
3VD㸧࡟ࡼࡗ࡚ᘬࡁ㉳ࡇࡉࢀࡿ⣽⳦ᛶࡢ⑓ᐖ࡛࠶ࡿࠋ3VDࡣ ࢟ ࢘ ࢖ ࣇ ࣝ ࣮ ࢶ 㸦 $FWLQLGLD FKLQHQVLV $
GHOLFLRVD㸧ࡢⴥࡸⰼⷣ࡟〓Ⰽᩬࢆᙧᡂࡋ㸪᪂ᲈࢆᯤṚࡉࡏࡿࠋࡉࡽ࡟㸪ᯞ㸪ᖿ࡟㉥〓Ⰽࡢᶞᾮ₃ฟࢆᘬࡁ㉳ࡇࡋ㸪᫬࡟㸪ᶞࢆᯤṚࡉࡏࡿࠋᮏ⑓ࡣ㢼㞵࡛㣕ᩓࡋ㸪㢼ᙜࡓࡾࡢᙉ࠸࡜ࡇࢁ࡛Ⓨ⏕ࡀከ࠸㸦∵ᒣ㸧ࠋ3VD ࡣከ
ᵝ ᛶ ࡟ ᐩ ࡳ 㸪 ᪥ ᮏ ࡛ ᭱ ึ ࡟ Ⓨ ぢ ࡉ ࢀ ࡓ ELRYDU㸦7DNLNDZDHWDO㸧ࡢ௚࡟ ✀㢮ࡢ ELRYDU ࡀ▱ࡽࢀ࡚࠸ࡿ㸦⃝⏣ࡽ㸪㸧ࠋࡑࡢ୰࡛ࡶ ELRYDU㸦௨ୗ㸪3VD㸧ࡣ㏆ᖺ㸪ୡ⏺࡛ὶ⾜ࢆᘬࡁ㉳ࡇࡋ㸪࢖ࢱࣜ࢔㸪ࢳࣜ㸪ࢽ࣮ࣗࢪ࣮ࣛࣥࢻ㸪୰ᅜ㸪㡑ᅜ➼୺せ⏕⏘ᅜ࡛☜ㄆࡉࢀ࡚࠸ࡿ㸦&XQW\ HWDO㸪㎰ᯘỈ⏘┬D㸧
ࠋ ᖺ ᭶࡟ឡ፾┴࡛ 3VD ࡀ᪥ᮏ࡛ึࡵ࡚Ⓨぢࡉࢀ㸪ྠ᫬ᮇ࡟⚟ᒸ┴㸪బ㈡┴㸪ᒸᒣ┴㸪࿴ḷᒣ┴㸪㟼ᒸ┴㸪Ⲉᇛ┴࡛ࡶ☜ㄆࡉࢀࡓ㸦⃝⏣ࡽ㸧ࠋࡑࡢᚋ㸪⚟ᒸ┴࡛ࡣᮏ⑓ࡢⓎ⏕ࡀᣑ኱ࡋ㸪⌧ᅾࡶ⿕ᐖࡀⓎ⏕ࡋ࡚࠸ࡿ㸦⳥ཎࡽ㸪㸧ࠋ࢟࢘࢖ࣇ࣮ࣝࢶࡣ㞤㞝␗ᰴ࡛㸪☜ᐇ࡞╔ᯝࡸᯝᐇ⫧኱ࢆ☜ಖࡍࡿࡓࡵ㸪ேᕤᤵ⢊ࡀ୍⯡ⓗ࡟⾜ࢃࢀ࡚࠸ࡿࠋࡑࢀ࡟౑⏝ࡉࢀࡿⰼ⢊ࡣ⮬ᐙ᥇ྲྀࡢ௚࡟ᑡ࡞ࡃ࡜ࡶ  ᖺ࠿ࡽࢽ࣮ࣗࢪ࣮ࣛࣥࢻ➼࠿ࡽ㍺ධࡉࢀ࡚࠸ࡿ㸦㎰ᯘỈ⏘┬D㸧ࠋᅜෆࡢ౵ධ⤒㊰ࡢ୍ࡘ࡜ࡋ࡚㸪ᾏእ࠿ࡽ㍺ධࡉࢀࡓ 3VD ࡟ởᰁࡉࢀࡓⰼ⢊㸦 ởᰁⰼ⢊㸧ࡢྍ⬟ᛶࡀ♧၀ࡉࢀࡓ 㸦㎰ᯘỈ⏘┬㸪⠛ᓮࡽ㸪኱㇂ࡽ㸧ࠋࡲࡓ㸪᥋✀ヨ㦂࡟ࡼࡾởᰁⰼ⢊ࡀ࠿࠸ࡼ࠺⑓ࡢఏᰁ※࡟࡞ࡾ࠺ࡿࡇ࡜ࡀሗ
࿌ࡉࢀ࡚࠸ࡿ㸦6WHIDQL࣭*LRYDQDUGL㸪7RQWRX HW
DO㸪⠛ᓮࡽ㸧ࠋ3VD ࡢ⑓ᐖࣜࢫࢡゎᯒࡢ⤖ᯝ㸪ࡇࢀ௨ୖࡢឤᰁᣑ኱ࢆ㜵Ṇࡍࡿࡓࡵ࡟ࡣ㸪೺඲ⰼ⢊
㸦3VD ࡟ởᰁࡉࢀ࡚࠸࡞࠸ⰼ⢊㸧࡟ࡼࡿᤵ⢊ࡀᚲせ࡛࠶ࡿ㸦㎰ᯘỈ⏘┬D㸧ࠋ⌧ᅾ㸪㍺ධ┦ᡭᅜࡢ᳨ᰝࢆ
㏻㐣ࡋࡓⰼ⢊ࡀ㍺ධࡉࢀ࡚࠸ࡿࡀ㸦㎰ᯘỈ⏘┬E㸧㸪㸨㐃⤡㈐௵⪅㸦⑓ᐖ⹸㒊㸸kikuhara@farc.pref.fukuoka.jp㸧       ཷ௜ 2018 ᖺ 7 ᭶ 27 ᪥㸹ཷ⌮ 2018 ᖺ 11 ᭶ 15 ᪥

6福岡県農林業総合試験場研究報告 ５（2019）㍺ධ┦ᡭᅜࡶ࠿࠸ࡼ࠺⑓ࡀⓎ⏕ࡋ࡚࠾ࡾ㸪೺඲ⰼ⢊ࡢ㍺
ධࡀ⥅⥆ࡉࢀࡿಖドࡣ࡞࠸ࠋࡇࡢࡓࡵ㸪ᮏ┴⏘ᆅ࡛ࡣⰼ⢊⏕⏘ࡀ᥎㐍ࡉࢀ࡚࠸ࡿࡀ㸪ᮏ┴ࡢ୺せ⏘ᆅࡣ࠿࠸ࡼ࠺
⑓ࡀⓎ⏕ࡋ㸪㞝ᮌࡢከࡃࡣᮏ⑓Ⓨ⏕ᆅᇦ࡟᳜᱂ࡉࢀ࡚࠸
ࡿࠋࢽ࣮ࣗࢪ࣮ࣛࣥࢻ࡟࠾࠸࡚㸪ᮏ⑓Ⓨ⏕ᅡሙ࡟࠶ࡿ㞝ᮌ࠿ࡽ᥇ྲྀࡉࢀࡓⰼ⢊ࡢởᰁ≧ἣࡀㄪᰝࡉࢀ㸪Ⓨ⑓ᶞࡔࡅ࡛࡞ࡃ㸪ᮍⓎ⑓ᶞ࠿ࡽ᥇ྲྀࡉࢀࡓⰼ⢊࠿ࡽࡶ 3VD ࡀ᳨ฟࡉࢀࡓ㸦03,㸧ࠋᮏ┴࡛ࡶྠᵝ࡟Ⓨ⏕ᅡሙ࠿ࡽ᥇ྲྀࡉࢀࡓⰼ⢊ࡀ 3VD ࡛ởᰁࡉࢀ࡚࠸ࡿ࡜᥎ᐃࡉࢀࡿࡀ㸪᪥ᮏᅜෆࡢㄪᰝ஦౛ࡢሗ࿌ࡀ࡞ࡃ㸪᝟ሗࡀ୙㊊ࡋ࡚࠸ࡿࠋ
ࡇࡢࡓࡵ㸪⚟ᒸࡢ࢟࢘࢖ࣇ࣮ࣝࢶࠕ⏑࠺࠷ࠖᬑཬ᥎㐍༠㆟఍㸦௨ୗ㸪ࠕ⏑࠺࠷ࠖ༠㆟఍㸧࡜ඹྠ࡛Ⓨ⏕ᆅᇦࡢ⏕⏘⪅ࡀ᥇ྲྀࡋࡓⰼ⢊ࡢởᰁ≧ἣࢆㄪᰝࡋࡓࠋᮏ┴࡛ࡣ㸪
ᮏ⑓ࡢⓎ⏕ㄪᰝࡢࡓࡵ㸪ⴥࡸᶞయ࠿ࡽࡢ᳨ฟࢆᐇ᪋ࡋ࡚
ࡁࡓࡀ㸦ᡂᒣࡽ㸧㸪ⰼ⢊ࡢㄪᰝࡣᐇ᪋ࡋ࡚࠸࡞࠿ࡗ
ࡓࠋⰼ⢊࠿ࡽࡢ 3VD ࡢ᳨ᐃ᪉ἲࡣࠕ࢟࢘࢖ࣇ࣮ࣝࢶ࠿
࠸ࡼ࠺⑓ 3VD ⣔⤫ࡢ㜵㝖ᑐ⟇࣐ࢽࣗ࢔ࣝ㸦௨ୗ㸪㜵㝖࣐ࢽࣗ࢔ࣝ㸧
ࠖ
㸦㎰ᯘỈ⏘┬E㸧࡟ᥖ㍕ࡉࢀ࡚࠸ࡿࡀ㸪 ࢧࣥࣉࣝࡈ࡜࡟㧗౯࡞ࢁ㐣⿦⨨ࢆ⏝࠸࡚ⰼ⢊ᠱ⃮ᾮ࠿ࡽ㞟⳦ࡋ࡚࠿ࡽ 3&5 ἲ᳨࡛ᐃࢆࡋ࡞ࡅࢀࡤ࡞ࡽ࡞࠸ࡓࡵ㸪኱㔞᳨ᐃ࡟ࡣ୙ྥࡁ࡛࠶ࡿࠋ୍᪉㸪๓㏙ࡢࢽ࣮ࣗࢪ࣮ࣛ
ࣥࢻࡢㄪᰝ㸦03,㸧ࡸ *DOOHOOLHWDO㸦㸧ࡣ
ᕷ㈍ࡢ '1$ ᢳฟ࢟ࢵࢺ࡛ởᰁⰼ⢊࠿ࡽ඲ '1$ ࢆᢳฟࡋ㸪3&5 ἲ᳨࡛ᐃࡋࡓࠋࡋ࠿ࡋ㸪᳨ฟ㝈⏺࡟ࡣゝཬࡋ࡚࠾ࡽࡎ 㸪 ᐮ ኳ ᇵ ᆅ ࡟ ࡼ ࡿ ศ 㞳 ࡛ ở ᰁ ⃰ ᗘ ࡀ   㹼FIXJ ࡜᥎ᐃࡉࢀࡓ⮬↛ởᰁⰼ⢊㸦03,㸧ࢆ࡝ࡢ⛬ᗘ᳨ฟྍ⬟࠿୙࡛᫂࠶ࡿࠋᮏ◊✲࡛ࡣⰼ⢊࠿ࡽ┤᥋ᢳฟࡋࡓ '1$ ࢆ㗪ᆺ࡜ࡍࡿ 3&5 ᪉ἲࡢ᳨ฟ㝈⏺ࢆ᳨ウࡋ㸪ởᰁⰼ⢊࠿ࡽࡢ 3VD ⡆᳨᫆ฟ᪉ἲࢆ☜❧ࡋࡓࠋࡉࡽ࡟㸪㛤Ⓨࡉࢀࡓᡭἲࢆ୰ᚰ࡟㸪ᮏ⑓ࡢⓎ⏕⛬ᗘࡢ␗࡞ࡿ⌧ᆅᅡሙ࠿ࡽ᥇ྲྀࡉࢀࡓⰼ⢊ࡢởᰁᐇែࢆㄪᰝࡋ㸪㞝ᮌࡀ࠶ࡿᅡሙ࠾ࡼࡧࡑࡢ࿘㎶ᅡሙ࡟࠾ࡅࡿᮏ⑓ࡢⓎ⏕≧ἣ࡜ⰼ⢊ࡢởᰁ≧ἣࡢ㛵㐃ᛶࢆゎᯒࡋࡓࠋ
ᮦᩱ࠾ࡼࡧ᪉ἲ
ヨ㦂㸯 ⰼ⢊࠿ࡽࡢ 3VD ᳨ฟ᪉ἲࡢ᳨ウ᳨ฟ㝈⏺ࢆ᳨ウࡍࡿࡓࡵ࡟ࡣ㸪ởᰁ⃰ᗘࡀ᫂ࡽ࠿࡞ⰼ⢊ࡀᚲせ࡛࠶ࡿࠋⓎ⏕ᶞ࠿ࡽ᥇ྲྀࡋࡓⰼ⢊࡛ࡣởᰁ⛬ᗘࡀ୙᫂࡞ࡓࡵ㸪ேᕤⓗ࡟ởᰁⰼ⢊ࢆస〇ࡋࡓࠋ㸯㸧ởᰁⰼ⢊ࡢస〇ởᰁⰼ⢊ࡢస〇࡟ࡣ  ᖺ࡟ࢽ࣮ࣗࢪ࣮ࣛࣥࢻ࠿ࡽ㍺

ධࡉࢀࡓ࢟࢘࢖ࣇ࣮ࣝࢶࡢⰼ⢊࠾ࡼࡧ  ᖺ࡟⚟ᒸ┴
ࡢᮍⓎ⏕ᆅᇦ࡛᥇ྲྀࡉࢀࡓ೺඲ⰼ⢊ࢆ⏝࠸ࡓࠋ⚟ᒸ┴࡛ ᖺ࡟ศ㞳ࡉࢀࡓ 3VD ⳦ᰴ 0$)) ࢆ๓ᇵ㣴ࡋࡓᚋ㸪ᬑ㏻ᐮኳᇵᆅ㸦ࢽࢵࢫ࢖㸪ᮾி㸧࡛ ᪥㛫ᇵ㣴ᚋ 㸪 ⳦ య ࢆ ᥇ ྲྀ ࡋ 㸪 ⁛ ⳦ Ỉ ࡛   㸪  㸪FIXP/ ࡟ㄪᩚࡋࡓࠋ࡞࠾㸪ㄪ〇ࡋࡓ⣽⳦ᠱ⃮ᾮ
ࢆᬑ㏻ᐮኳᇵᆅ࡟ሬᕸࡋ㸪 ᪥ᚋ࡟ࢥࣟࢽ࣮ᩘࢆィ ࡋ࡚⣽⳦⃰ᗘࢆ☜ㄆࡋࡓࠋⰼ⢊ J ࢆ P/ ࡢࢳ࣮ࣗࣈ࡟ධࢀ㸪ྛ⃰ᗘࡢ⣽⳦ᠱ⃮ᾮࢆ w/ ຍ࠼㸪㸪㸪FIXJ ┦ᙜࡢởᰁⰼ⢊ࢆస〇ࡋࡓࠋ ᅇࡢヨ㦂࡟ ཯᚟స〇ࡋ㸪ᐇ㦂ࢆ ᅇ⧞ࡾ㏉ࡋࡓࠋ
㸰㸧'1$ ࡢᢳฟᡂᒣࡽ㸦㸧ࡢ᪉ἲ࡜ྠᵝ࡟㸪'1$ ࡢᢳฟࡣ☢ᛶࣅ࣮ࢬࢆ⏝࠸ࡓ 0DJ([WUDFWRU3ODQWJHQRPH㸦72<2%2㸪኱㜰㸧ࢆ౑⏝ࡋ㸪ᡭ㡰ࡣῧ௜ࡢ࣐ࢽࣗ࢔ࣝ࡟ᚑࡗࡓࠋ࡞
࠾㸪ⰼ⢊ࡣ⢊ᮎ࡛࠶ࡿࡓࡵ㸪ᾮయ❅⣲ࢆ⏝࠸ࡓ෾⤖◚○ࢆ┬␎ࡋࡓࠋ
㸱㸧3&5 ཯ᛂࡢ᮲௳
 3&5 ཯ ᛂ ࡟ ࡣ $PSOL7DT *ROG  0DVWHU 0L[DSSOLHGELRV\VWHPV㸪ࣇ࢛ࢫࢱ࣮ ࢆ౑⏝ࡋࡓࠋῧ௜ࡢ
ㄝ᫂᭩ࢆཧ⪃࡟඲཯ᛂᾮࢆ w/ ࡟ㄪ〇ࡋࡓࠋேᕤởᰁⰼ ⢊ ࠿ ࡽ ᢳ ฟ ࡋ ࡓ '1$ ࠾ ࡼ ࡧ   㸪  㸪FIXP/ ࡟ㄪᩚࡋࡓ⣽⳦ᠱ⃮ᾮࡑࢀࡒࢀw/ ࢆ㗪ᆺ࡜ࡋ࡚⏝࠸ࡓࠋ㜵㝖࣐ࢽࣗ࢔ࣝ࡟ᥖ㍕ࡉࢀ࡚࠸ࡿࣉࣛ࢖ ࣐ ࣮ ࢭ ࢵ ࢺ 㸪 36$ )36$ 5 㸦 5HHV*HRUJH HW DO㸧㸦௨ୗ㸪ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮㸪➨ ⾲㸧㸪3 KRS$ )3 KRS$ 5㸦ΎỈࡽ㸧㸦௨ୗ㸪KRS$ࣉࣛ࢖࣐࣮㸪➨ ⾲㸧࠾ࡼࡧ KRS=)KRS=5㸦⃝⏣ࡽ㸧㸦௨ୗ㸪KRS= ࣉࣛ࢖࣐࣮㸪➨ ⾲㸧ࢆ⏝࠸ࡓࠋࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮࡜ KRS$ ࣉࣛ࢖࣐࣮ࢆ⏝࠸ࡓ
3&5 ᮲௳ࡣ㸪Υ ศ㸪㸦Υ ⛊㸪Υ ⛊㸪Υ⛊㸧ࢆ  ᅇ⧞ࡾ㏉ࡋ㸪Υ ศ࡜ࡋࡓࠋKRS= ࣉࣛ࢖࣐࣮ࢆ⏝࠸ࡓ 3&5 ᮲௳ࡣ㸪Υ ศ㸪㸦Υ ⛊㸪Υ⛊㸪Υ ⛊㸧ࢆ  ᅇ⧞ࡾ㏉ࡋ㸪Υ ศ࡜ࡋࡓࠋቑ
ᖜ '1$ ᩿∦ࢆ㸣ࡢ࢔࣮࢞ࣟࢫࢤࣝ㟁ẼὋື࡛☜ㄆࡋࡓࠋ

ヨ㦂㸰 ⌧ᆅ࡛᥇ྲྀࡉࢀࡓⰼ⢊࡟࠾ࡅࡿ 3VD ࡢởᰁ≧ἣ ᮏㄪᰝࡣࠕ⏑࠺࠷ࠖ༠㆟఍࡜ศᢸࡋ࡚ᐇ᪋ࡉࢀࡓࠋ
㸯㸧ⰼ⢊ࡢ཰㞟࡜᳨ฟ᪉ἲ  ᖺ࡟⚟ᒸ┴ $ ᕷࡢ⏕⏘⪅  ேࡀ᥇ྲྀࡋࡓ  ᳨యࡢⰼ⢊ࢆ  ᖺ ᭶࡟཰㞟ࡋ㸪ヨ㦂࡟⏝࠸ࡓࠋࡇࢀ

➨㸯⾲ ᮏ◊✲࡛౑⏝ࡋࡓࣉࣛ࢖࣐࣮㓄ิࣉࣛ࢖࣐࣮ྡ
ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮
PsaF1
PsaR2
hopA ࣉࣛ࢖࣐࣮
P0(hopA)-F1
P3(hopA)-R2
hopZ ࣉࣛ࢖࣐࣮
hopZ5-F2
hopZ5-R2

ࣉࣛ࢖࣐࣮㓄ิ㸦5’Ѝ3’㸧
TTTTGCTTTGCACACCCGATTTT
CACGCACCCTTCAATCAGGATG

ࢧ࢖ࢬ(bp)280

ฟ඾Rees-George et al.

㸦2010㸧GCAGAGGCGATGCAAAACCCG
ACGCGACAACTACCTTGAAAAACG

186

ΎỈࡽ
㸦2015㸧CAGGAATTCATGACTTCTCATAGTCTCGAAGATTCAATGG

630

⃝⏣ࡽ
㸦2015㸧

キウイフルーツかいよう病菌（Pseudomonas syringae pv. actinidiae (Psa)）に汚染された花粉からのPsaの検出方法の開発および福岡県内で採取された花粉の汚染状況調査ࡽ  ᳨యࡢ࠺ࡕ  ᳨యࡢ᳨ᰝࢆࣄ࣭ࣝࣛ࣎ࣛࢺ࣮ࣜࢬ
㸦ࢽ࣮ࣗࢪ࣮ࣛࣥࢻ㸧࡟ጤクࡋࡓࠋࣄ࣭ࣝࣛ࣎ࣛࢺ࣮ࣜ
ࢬ࡛ࡣ㸪☢ᛶࣅ࣮ࢬࢆ⏝࠸࡚ '1$ ࢆᢳฟࡋ㸪ࣜ࢔ࣝࢱ࢖
࣒ 3&5 ἲ᳨࡛ᐃࡋࡓࠋ ࢧࣥࣉࣝ࠶ࡓࡾ FIX ࡀ᳨ฟ㝈⏺࡛࠶ࡿࠋ࡞࠾㸪3&5 ࣉࣛ࢖࣐࣮ࡣබ⾲ࡉࢀ࡚࠸࡞࠸ࠋṧࡾࡢ  ᳨యࢆ௨ୗࡢ᪉ἲ᳨࡛ᰝࡋࡓࠋ ࢧࣥࣉࣝ࠶ࡓࡾ J ࢆ P/ ࡢࢳ࣮ࣗࣈ࡟ධࢀ㸪๓㏙ࡢ᪉ἲ࡛㸪'1$ ࢆᢳฟࡋ㸪3&5 ἲࢆᐇ᪋ࡋࡓࠋࣉࣛ࢖࣐࣮ࡣᚋ㏙ࡢ⤖ᯝࡼࡾ㸪ேᕤởᰁⰼ⢊࠾ࡼࡧ⣽⳦ᠱ⃮ᾮ࠿ࡽࡢ᳨ฟ㝈⏺ࢆ⪃៖ࡋ㸪ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮࡜ KRS$ ࣉࣛ࢖࣐࣮ࢆ⏝࠸ࡓࠋࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮ࡀ㏆⦕✀ࡢࢳࣕ㉥↝⑓⳦㸦3VHXGRPRQDVV\ULQJDHSYWKHDH㸧࡟཯ᛂࡍࡿ
㸦5HHV*HRUJH HWDO㸧ࡼ࠺࡟㸪3&5 ᳨ᐃ࡟ࡣഇ
㝧ᛶ཯ᛂࡢ༴㝤ᛶࢆక࠺ࡓࡵ㸪」ᩘࡢࣉࣛ࢖࣐࣮ࢭࢵࢺ
ࢆ⏝࠸㸪୧ࣉࣛ࢖࣐࣮ࢭࢵࢺࡢ཯ᛂ⤖ᯝࡀ୍⮴ࡋࡓ࡜ࡁ࡟㝧ᛶ࡜ࡋࡓࠋ ᳨య࠶ࡓࡾ ཯᚟ᐇ᪋ࡋࡓࠋ3VD ࡀΰධࡋࡓⰼ⢊࡛࠶ࡗ࡚ࡶ㸪അ↛ 3VD ࡀᏑᅾࡋ࡞࠸㒊ศࢆᢳฟࡍࡿྍ⬟ᛶࡀ࠶ࡿࡓࡵ㸪㝧ᛶࡀ ཯᚟࡛ࡶ☜ㄆࡉࢀࡓሙྜ㸪ࡑࡢ᳨యࢆ㝧ᛶ࡜ࡋࡓࠋ
㸰㸧⮬ᐙ᥇ྲྀⰼ⢊ࡢ᮶Ṕㄪᰝ⌧ᆅࡢ㞝ᮌࡀ࠶ࡿᅡሙ࠾ࡼࡧࡑࡢ࿘㎶ᅡሙࡢ࠿࠸ࡼ࠺
⑓Ⓨ⏕≧ἣ࡜ⰼ⢊ࡢởᰁ≧ἣࡢ㛵㐃ᛶࢆㄪᰝࡍࡿࡓࡵ㸪㞝ᮌࡀ࠶ࡿᅡሙ࠾ࡼࡧࡑࡢ㞄᥋ᅡሙ࡟࠾ࡅࡿᮏ⑓ࡢⓎ⏕ࡢ᭷↓࡟ࡘ࠸࡚㸪 ᖺ⏘ࡢ⏕⏘⤊஢ᚋ࡟⏕⏘⪅࡬ࡢ⪺ࡁྲྀࡾㄪᰝࢆᐇ᪋ࡋࡓࠋࡉࡽ࡟㸪㞝ᮌࡀ࠶ࡿᅡሙࡢᆅ
༊ࢆ኱Ꮠ༢఩㸦ᩘ NP ⛬ᗘ㸧࡛ ᆅ༊࡟ࢃࡅ㸪ᆅ༊ࡈ࡜
ࡢᮏ⑓Ⓨ⏕㠃✚⋡ࢆ  ᖺࡢ᫓Ꮨㄪᰝ㸦㎰ᯘỈ⏘┬㸪E㸧⤖ᯝ࠿ࡽྲྀࡾࡲ࡜ࡵࡓࠋࡇࢀࡽࡢ᝟ሗࢆᇶ࡟㸪㞝ᮌ࡜ᮏ⑓Ⓨ⏕ᅡሙࡢ㛵ಀ࡟ᛂࡌ࡚㸪ởᰁࣜࢫࢡࢆ
ẁ㝵࡟༊ศࡋࡓ㸦➨ ᅗ㸧ࠋࡍ࡞ࢃࡕ㸪㞝ᮌࡀ↓Ⓨ⏕ᆅ༊࡟࠶ࡿሙྜࢆࣜࢫࢡ㸪ᮏ⑓Ⓨ⏕ᆅ༊࡛㸪㞝ᮌࡢ࠶ࡿ
ᅡሙ࡟Ⓨ⑓ᶞࡀ࠶ࡿሙྜࢆࣜࢫࢡ㸪㞝ᮌࡀ࠶ࡿᅡሙ࡟
Ⓨ⑓ᶞࡣ↓࠸ࡀ㞄᥋ᅡሙ࡟Ⓨ⑓ᶞࡀ࠶ࡿሙྜࢆࣜࢫࢡ㸪㞝ᮌࡀ࠶ࡿᅡሙ࡜ࡑࡢ㞄᥋ᅡሙ࡛Ⓨ⑓ᶞࡀ࡞࠸ሙྜࢆࣜ
ࢫࢡ ࡜ࡋࡓࠋ࡞࠾㸪᫓Ꮨㄪᰝࡣ┴ෆ඲ᆅᇦࢆᑐ㇟࡟ࣜࢫࢡホ౯ࡢ㛤ጞ

㞝ᮌࡣⓎ
⏕ᆅ༊࡟
࠶ࡿ

࠸࠸࠼

⏕⏘⪅㸪ᬑཬᣦᑟဨ࠾ࡼࡧ -$ Ⴀ㎰ᣦᑟဨ࠿ࡽࡢデ᩿౫㢗ࢆඖ࡟ᐇ᪋ࡉࢀ࡚࠸ࡿࠋ⤖ ᯝヨ㦂㸯 3VD ᳨ฟ᪉ἲࡢ᳨ウ⣽⳦ᠱ⃮ᾮࢆ㗪ᆺ࡟ࡋ᳨࡚ฟࡋࡓ⤖ᯝ㸪ࣘࢽࣂ࣮ࢧࣝ
ࣉࣛ࢖࣐࣮࠾ࡼࡧ KRS$ ࣉࣛ࢖࣐࣮ࡣ FIXP/㸦ࢧ ࣥ ࣉ ࣝ ࠶ ࡓ ࡾ FIX 㸧 ࡲ ࡛ 㸪 KRS= ࣉ ࣛ ࢖ ࣐ ࣮ ࡣFIXP/㸦 ࢧࣥࣉࣝ࠶ࡓࡾ FIX㸧ࡲ᳨࡛ฟࡉࢀࡓ
㸦➨ ᅗ㸧ࠋேᕤởᰁⰼ⢊࠿ࡽᢳฟࡋࡓ '1$ ࢆ㗪ᆺ࡟ࡋ᳨࡚ฟࡋࡓ⤖ᯝ㸪ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮ࡣ FIXJ㸦 ࢧࣥࣉࣝ࠶ࡓࡾ FIX㸧ࡲ᳨࡛ฟࡉࢀ㸪KRS$ࣉࣛ࢖࣐࣮࠾ࡼࡧ KRS= ࣉࣛ࢖࣐࣮ࡣFIXJ㸦ࢧࣥࣉࣝ࠶ࡓࡾ FIX㸧ࡲ᳨࡛ฟࡉࢀࡓࠋヨ㦂ࢆ ᅇᐇ᪋ࡋࡓࡀ㸪࠸ࡎࢀࡢࢧࣥࣉࣝࡶ⤖ᯝࡣྠࡌ࡛㸪෌⌧ᛶa) ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮
500 bpேᕤởᰁⰼ⢊ ⣽⳦ᠱ⃮ᾮ
M 1 2 3 H 4 5 6 W

300 bpb) hopAࣉࣛ࢖࣐࣮
M 1 2
500 bp

3 H

4

5 6 W

3 H

4

5 6

200 bpc) hopZࣉࣛ࢖࣐࣮
M 1 2
700 bpW

500 bp

ࡣ࠸

㞝ᮌࡣⓎ
⏕ᅡሙ࡟
࠶ࡿ
ࡣ࠸

࠸࠸࠼

㞝ᮌᅡሙ
ࡢ㞄᥋ᅡ
ሙ࡟Ⓨ⏕
ࡋ࡚࠸ࡿ

࠸࠸࠼

ࡣ࠸
ࣜࢫࢡ㸮

7

ࣜࢫࢡ㸱

ࣜࢫࢡ㸰

ࣜࢫࢡ㸯



➨㸯ᅗ 㞝ᮌࡀ࠶ࡿᅡሙࡢࣜࢫࢡホ౯ࡢࣇ࣮ࣟࢳ࣮ࣕࢺ

➨㸰ᅗ ྛ✀ࣉࣛ࢖࣐࣮ࢆ⏝࠸ࡓ 3&5 ἲ࡟ࡼࡿቑᖜ
⏘≀ࡢ㟁ẼὋືീ
D ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮㸦5HHV*HRUJHHWDO
㸧㸹E KRS$ ࣉࣛ࢖࣐࣮㸦ΎỈࡽ㸧㸹& KRS= ࣉࣛ࢖࣐࣮㸦⃝⏣ࡽ㸧0㸸ES ࣛࢲ࣮࣐࣮࣮࢝㸪㹼㸸ேᕤởᰁⰼ⢊㸪㸸 FIXJ㸪㸸 FIXJ㸪㸸 FIXJ㸪+㸸೺඲ⰼ⢊㸪㹼㸸⣽⳦ᠱ⃮ᾮ㸪㸸 FIXP/㸪㸸 FIXP/㸪㸸 FIXP/㸪:㸸⁛⳦Ỉ

8

福岡県農林業総合試験場研究報告 ５（2019）༊ࡢ᳨య࠿ࡽ 3VD ࡀ᳨ฟࡉࢀࡓࡀ㸪୧ᆅ༊ࡼࡾⓎ⏕㠃✚ࡀᑡ࡞࠸ & ᆅ༊࠾ࡼࡧ ' ᆅ༊ࡢ᳨య࠿ࡽࡣ᳨ฟࡉࢀ࡞࠿ࡗࡓࠋⓎ⏕ᆅ༊࡛ࡣ࡞࠸ࣜࢫࢡ ࠿ࡽࡣ᳨ฟࡉࢀ࡞
࠿ࡗࡓࠋࡀ㧗࠿ࡗࡓࠋ

ヨ㦂㸰 ⌧ᆅ࡛᥇ྲྀࡉࢀࡓⰼ⢊࡟࠾ࡅࡿ 3VD ࡢởᰁ≧ἣ ᖺ࡟࠾ࡅࡿྛᆅ༊ࡢⓎ⏕ᅡሙ㠃✚⋡ࡣ $ ᆅ༊࡛㸣㸪% ᆅ༊࡛ 㸣㸪& ᆅ༊࡛ 㸣㸪' ᆅ༊࡛㸣㸪( ᆅ༊࡛ 㸣࡛࠶ࡗࡓ㸦➨ ᅗ㸧ࠋ⌧ᆅ࡛᥇ྲྀࡉࢀࡓⰼ⢊ࢆ᳨ᐃࡋࡓ⤖ᯝ㸪$ ᆅ༊  ᳨యࡢ࠺ࡕ ᳨య㸦࠺ࡕ㸪ᮏ㛤Ⓨᡭἲ࡛ࡣ  ᳨యࡢ࠺ࡕ ᳨య㸧
㸪% ᆅ༊  ᳨యࡢ࠺ࡕ ᳨య㸦࠺ࡕ㸪ᮏ㛤Ⓨᡭἲ࡛ࡣ ᳨యࡢ࠺ࡕ ᳨య㸧㸪& ᆅ༊ ᳨యࡢ࠺ࡕ
᳨య㸦࠺ࡕ㸪ᮏ㛤Ⓨᡭἲ࡛ࡣ ᳨యࡢ࠺ࡕ ᳨య㸧࠿
ࡽ 3VD ࡀ᳨ฟࡉࢀࡓࠋ୍᪉㸪' ᆅ༊ ᳨య㸦࠺ࡕ㸪ᮏ
㛤Ⓨᡭἲ࡛ࡣ ᳨య㸧࠾ࡼࡧ ( ᆅ༊ ᳨య㸦࠺ࡕ㸪ᮏ
㛤Ⓨᡭἲ࡛ࡣ ᳨య㸧࠿ࡽࡣࡍ࡭᳨࡚ฟࡉࢀ࡞࠿ࡗࡓࠋ
ᆅ༊࡟࠾ࡅࡿᮏ⑓Ⓨ⏕ᅡሙ⋡ࡀ㧗࠸࡯࡝᳨ฟ⋡ࡀ㧗࠸ഴྥࡀ࠶ࡗࡓ㸦➨ ᅗ㸧ࠋࡲࡓ㸪ᮏ㛤Ⓨᡭἲ࡟ࡼࡿ᳨ฟ๭
ྜ㸦 ᳨య ᳨య㸧ࡣࣄ࣭ࣝࣛ࣎ࣛࢺ࣮ࣜࢬ࡟ࡼࡿ᳨
ฟ๭ྜ㸦᳨య ᳨య㸧ࡼࡾ኱ࡁ࠿ࡗࡓࠋࡲࡓ㸪ࣜࢫࢡ ࡣᆅ༊࡟㛵ࢃࡽࡎࡍ࡭࡚ࡢ᳨య࠿ࡽ 3VD ࡀ᳨ฟࡉࢀࡓࠋࣜࢫࢡ ࠾ࡼࡧࣜࢫࢡ ࡣ $ ᆅ༊࠾ࡼࡧ % ᆅ
100%
࠿
࠸
ࡼ
࠺
⑓ 50%
⳦
㝧
ᛶ
⋡

0
3
(0)
5
(5)
3
(3)

⪃ ᐹ
ேᕤởᰁⰼ⢊࡟࠾ࡅࡿࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮ࡢ 3VDࡢ᳨ฟ㝈⏺㸦FIXJ㸪 ࢧࣥࣉࣝ࠶ࡓࡾFIX㸧ࡣ KRS$ ࣉ ࣛ ࢖ ࣐ ࣮ ࠾ ࡼ ࡧ KRS= ࣉ ࣛ ࢖ ࣐ ࣮㸦FIXJ㸪 ࢧࣥࣉࣝ࠶ࡓࡾ FIX㸧ࡼࡾඃࢀࡓࠋ
⣽⳦ᠱ⃮ᾮ࡟࠾ࡅࡿࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮࠾ࡼࡧKRS$ ࣉࣛ࢖࣐࣮ࡢ᳨ฟ㝈⏺㸦FIXP/㸪 ࢧࣥࣉࣝ࠶ࡓࡾFIX㸧ࡣ KRS= ࣉࣛ࢖࣐࣮㸦FIXP/㸪ࢧࣥࣉࣝ࠶ࡓࡾ FIX㸧ࡼࡾඃࢀࡓࠋ௨ୖࡢ⤖ᯝ㸪ࣘࢽࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮㸪KRS$ ࣉࣛ࢖࣐࣮㸪KRS= ࣉࣛ࢖࣐࣮ࡢ㡰࡟᳨ฟຊࡀ㧗࠸࡜᥎ᐃࡉࢀࡓࠋࡉࡽ࡟㸪ேᕤởᰁⰼ⢊࠾ࡼࡧ⣽⳦ᠱ⃮ᾮ࡜ࡶ࡟᳨ฟຊࡀ୍␒㧗࠿ࡗࡓࣘࢽ
ࣂ࣮ࢧࣝࣉࣛ࢖࣐࣮࡜ḟ࠸࡛㧗࠿ࡗࡓ KRS$ ࣉࣛ࢖࣐࣮
ࡢ᳨ฟ㝈⏺ࡣ㸪ࣄ࣭ࣝࣛ࣎ࣛࢺ࣮ࣜࡢᡭἲ㸦 ࢧࣥࣉࣝ࠶ࡓࡾ FIX㸧࡜ẚ㍑ࡋ࡚ྠ➼࠿ࡽඃࢀ࡚࠸ࡓࠋୖグࡢ⤖ᯝࢆᇶ࡟㸪⌧ᆅⰼ⢊ࡢởᰁ≧ἣㄪᰝ࡛ࡣࣘࢽࣂ࣮ࢧ

100%

0

0

࠿
࠸
ࡼ
(1)
࠺
⑓ 50%
⳦
㝧
1 ᛶ
(1) ⋡

2

2

(0)

(1)

1

0%

0%

㝧ᛶ

(1)

ࣜࢫࢡ3 ࣜࢫࢡ2 ࣜࢫࢡ1Aᆅ༊㸦Ⓨ⏕ᅡሙ⋡13.2%㸧

࠿
࠸
ࡼ
࠺
⑓ 50%
⳦
㝧
ᛶ
⋡

㝜ᛶ

(1)

1

0%
ࣜࢫࢡ3 ࣜࢫࢡ2 ࣜࢫࢡ1

100%

5

Bᆅ༊
㸦6.6%㸧

0

100%

100%

1

1

2

(1)

(0)

(1)

࠿
࠸
ࡼ
࠺
⑓ 50%
⳦
㝧
ᛶ
⋡

䛛
䛔
䜘
䛖
⑓ 50%
⳦
㝧
ᛶ
⋡

0
0
ࣜࢫࢡ3 ࣜࢫࢡ2 ࣜࢫࢡ1Cᆅ༊㸦Ⓨ⏕ᅡሙ⋡4.6%㸧

0%

3

3

(3)

(1)

0
0
ࣜࢫࢡ2 ࣜࢫࢡ10%Dᆅ༊
㸦3.4%㸧

3
(1)

0
ࣜࢫࢡ
Eᆅ༊
㸦0%㸧

➨㸱ᅗ 㞝ᮌࡀ࠶ࡿᅡሙࡢྛࣜࢫࢡูࡢ࠿࠸ࡼ࠺⑓⳦᳨ฟ⋡ ࢢࣛࣇෆࡢᩘ್ࡣ᳨ᐃᩘ࡛㸪㸦㸧ෆࡣ㸪ࡑࡢ࠺ࡕᮏ᳨ฟ᪉ἲ࡛ᐇ᪋ࡋࡓᩘ

キウイフルーツかいよう病菌（Pseudomonas syringae pv. actinidiae (Psa)）に汚染された花粉からのPsaの検出方法の開発および福岡県内で採取された花粉の汚染状況調査

ࣝࣉࣛ࢖࣐࣮࠾ࡼࡧ KRS$ ࣉࣛ࢖࣐࣮ࢆ⏝࠸ࡓࠋࡑࡢ⤖ᯝ㸪ᮏ㛤Ⓨᡭἲࡣࣄ࣭ࣝࣛ࣎ࣛࢺ࣮ࣜࢬࡢᡭἲࡼࡾ᳨ฟ
๭ྜࡀ㧗࠸ഴྥࡀ࠶ࡗࡓࠋ≉࡟㸪$ ᆅ༊ࡢࣜࢫࢡ ࡜ %ᆅ༊ࡢࣜࢫࢡ ࡢ㝜ᛶࡢከࡃࡣࣄ࣭ࣝࣛ࣎ࣛࢺ࣮ࣜࢬ࡟ጤクࡋ࡚࠾ࡾ㸪$ ᆅ༊࡜ % ᆅ༊ࡢ᳨ฟ๭ྜࡣࡉࡽ࡟㧗࠸ ྍ ⬟ ᛶ ࡀ ࠶ ࡗ ࡓ  ࡲ ࡓ 㸪 ᮏ 㛤 Ⓨ ᡭ ἲ 㸦 ᳨ ฟ 㝈 ⏺ ࡣFIXJ㸧ࡣ⮬↛ Ⓨ ⑓ᶞ ࠿ ࡽ᥇ ྲྀ ࡉࢀ ࡓở ᰁⰼ⢊㸦ởᰁ⃰ᗘ 㹼FIXJ㸦03,㸧㸧ࢆ࡯ࡰ᳨ฟྍ⬟࡜⪃࠼ࡽࢀࡓࡓࡵ㸪ⰼ⢊᳨ᐃ࡟᭷ຠ࡞᳨ฟ᪉ἲ
࡛࠶ࡿ࡜᥎ᐹࡉࢀࡓࠋຍ࠼࡚㸪㜵㝖࣐ࢽࣗ࢔ࣝ࡟ᥖ㍕ࡉࢀ࡚࠸ࡿởᰁⰼ⢊ࡢ᳨ᐃ᪉ἲࡢ᳨ฟ㝈⏺ࡣ୙࡛᫂࠶ࡿࡀ㸪ᮏᡭἲࡣ㧗౯࡞ᶵჾࢆᚲせ࡜ࡋ࡞࠸ࡓࡵ㸪኱㔞᳨ᐃ࡟㐺ࡍࡿ࡜⪃࠼ࡽࢀࡓࠋ
௨ୖࡢ⤖ᯝࢆ⪃៖ࡋ㸪ᮏ⑓ࡢⓎ⏕≧ἣ࡜ⰼ⢊࠿ࡽࡢ
3VD ᳨ฟ๭ྜ࡟ࡘ࠸࡚⪃ᐹࡋࡓࠋ⌧ᆅ࡛᥇ྲྀࡉࢀࡓⰼ
⢊ࡢởᰁ≧ἣㄪᰝࡢ⤖ᯝ㸪ᮏ⑓↓Ⓨ⏕ᆅ༊ࡢởᰁࣜࢫࢡࡣ㠀ᖖ࡟పࡃ㸪ᆅ༊ࡢⓎ⏕㠃✚⋡ࡀ㧗࠸࡯࡝㸪ởᰁࣜࢫࢡࡀ㧗࠸ഴྥࡀ࠶ࡗࡓࠋᮏ⑓Ⓨ⏕ᆅ༊࡛ࡣ㸪㞝ᮌࡀ࠶ࡿᅡሙࡢ㏆ࡃ࡟Ⓨ⏕ᅡሙࡀ࠶ࡿ࡯࡝ởᰁࣜࢫࢡࡀ㧗ࡃ㸪࿘㎶ᅡሙ࡟Ⓨ⏕ࡀ☜ㄆࡉࢀ࡞ࡃ࡚ࡶởᰁࣜࢫࢡࡀᏑᅾࡋ࡚
࠸ࡓࠋ୍᪉㸪& ᆅ༊࠾ࡼࡧ ' ᆅ༊࡛ࡣ㞄᥋ᅡሙ࡟Ⓨ⏕ࡋ࡚࠸࡚ࡶ 3VD ࡀ᳨ฟࡉࢀ࡞࠿ࡗࡓࠋࡇࢀࡽࡢᆅ༊ࡣ
3VD ࡀ᳨ฟࡉࢀࡓ % ᆅ༊ࡼࡾࡶⓎ⏕㠃✚⋡ࡀᑡ࡞࠸ࡶ
ࡢࡢᩘ㸣ࡢᕪࡋ࠿࡞ࡃ㸪௒ᅇࡢࣜࢫࢡホ౯ᇶ‽࡛ࡣࡇࢀࡽࢆ༊ู࡛ࡁ࡞࠿ࡗࡓࠋởᰁࣜࢫࢡࢆヲ⣽࡟ホ౯ࡍࡿ࡟ࡣ㸪ᮏ⑓ࡢⓎ⏕ᣑ኱ࢆᢚไࡍࡿ㜵㢼ᯘࡢᏑᅾࡸᅡሙ࿘㎶ࡢᆅᙧ➼ࡢࡉࡽ࡞ࡿ࿘㎶᝟ሗࡢ཰㞟࡜ㄪᰝ஦౛ࡀᚲせ࡜⪃࠼ࡽࢀࡓࠋᮏ◊✲࡛ࡣⓎ⑓ࡋ࡚࠸࡞࠸㞝ᮌ࠿ࡽ 3VDࡀ᳨ฟࡉࢀࡓࠋࡲࡓ㸪ᮏㄪᰝ࡟ඛ⾜ࡋ࡚ᐇ᪋ࡋࡓⰼ⢊᳨ᐃ࡛ࡣ㸪㞝ᮌࡢึⓎࢆ☜ㄆࡋࡓ๓ᖺ࡟᥇ྲྀࡋࡓⰼ⢊࠿ࡽ
3VD ࡀ᳨ฟࡉࢀࡓ㸦ࢹ࣮ࢱ␎㸧ࠋᮏ◊✲࡛ㄪᰝࡉࢀࡓ㞝ᮌ㸦$GHOLFLRVD㸧ࡣ 3VD ࡟ࡼࡿⴥࡸᯞࡢⓎ⑓࡟ᑐࡋ࡚ẚ㍑ⓗᙉ࠸ရ✀࡛࠶ࡿ㸦㎰ᯘỈ⏘┬E㸧ࠋⰼ⢊ࡢởᰁ࡜ᮏ⑓࡟ࡼࡿ⑕≧ࡸⓎ⑓ࢆᣦᶆ࡜ࡋࡓ᢬ᢠᛶࡣ㛵㐃ᛶࡀప࠸ྍ⬟ᛶࡀ࠶ࡿࠋᮏ᳨ᰝ⤖ᯝ࠿ࡽ㸪㞝ᮌࡢ࿘㎶ᅡ
ሙࡢⓎ⏕≧ἣ࡜ⰼ⢊ࡢởᰁࣜࢫࢡ࡟୍ᐃࡢ㛵㐃ᛶࡀ☜ㄆࡉࢀࡓࠋࡋ࠿ࡋ㸪Ⓨ⏕ᆅ༊࡛ởᰁࣜࢫࢡࡀ࡞࠸ⰼ⢊ࢆ༊ูࡍࡿࡇ࡜ࡣ࡛ࡁ࡞࠿ࡗࡓࡓࡵ㸪Ⓨ⏕ᆅ༊࡛ࡣởᰁࣜࢫࢡࡢ࡞࠸ⰼ⢊ࢆ⏕⏘ࡍࡿࡇ࡜ࡣᅔ㞴࡛࠶ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋࡇࡢࡇ࡜࠿ࡽ㸪ᤵ⢊⏝ࡢⰼ⢊ࡣ㸪ᑡ࡞ࡃ࡜ࡶ㸪ࣜࢫࢡ ࡍ࡞ࢃࡕ㸪ᮏ⑓↓Ⓨ⏕ᆅ༊࡛⏕⏘ࡍࡿࡇ࡜ࡀᚲ
せ࡜ࡉࢀࡿࠋ
ᮏ┴࡛ࡣ  ᖺ௨㝆㸪3VD ࡟᢬ᢠᛶࡀᙅ࠸ࣞ࢖ࣥ࣎
࣮ࣞࢵࢻ㸦㎰㣗஦ᴗ & ࢥࣥࢯ࣮ࢩ࢔࣒㸪㸧ࢆ୰ᚰ࡟ᮏ⑓ࡢ⿕ᐖࡀᣑ኱ࡋ࡚࠸ࡿ㸦⳥ཎࡽ㸪㸧ࠋ୍᪉㸪ᮍⓎ⏕ᅬࡶᏑᅾࡋ࡚࠾ࡾ㸪ࡇࢀ௨ୖࡢⓎ⏕ᣑ኱ࢆ㜵ࡄࡓࡵ㸪೺඲ⰼ⢊ࡢ౑⏝ࡣ㔜せ࡛࠶ࡿࠋ㍺ධⰼ⢊࡟౫Ꮡࡋࡓⰼ⢊ࡢ౪⤥యไ࡟ࡣ㸪 ᖺᗘ࡟㍺ධⰼ⢊࠿ࡽ
3VD ࡀ᳨ฟࡉࢀ㸪㍺ධ࡛ࡁ࡞࠸஦౛ࡀⓎ⏕ࡍࡿ㸦㎰ᯘỈ⏘┬㸪E㸧࡞࡝㸪୙ ࡢࣜࢫࢡࡀ࠶ࡿࠋࡇࡢࡓࡵ㸪୍ᐃ㔞ࡢ⮬⤥ࡣᚲせ࡛࠶ࡿࡀ㸪┴ෆࡢ୺࡞⏘ᆅࡣᮏ⑓ࡀ
Ⓨ⏕ࡋ࡚࠾ࡾ㸪೺඲ⰼ⢊ࡢ⏕⏘ࡀᅔ㞴࡛࠶ࡿࠋ௒ᚋࡢ◊✲ࡣ㸪ᮏ⑓ᮍⓎ⏕ࡢ᪂᳜ᆅ࡛▷ᮇ㛫࡟ⰼ⢊ࢆ⏕⏘࡛ࡁࡿ

9᱂ᇵἲࡸⰼ⢊⏕⏘࡟㐺ࡍࡿရ✀㛤Ⓨ࣭㑅ᢤࡀᚲせ࡛࠶ࡿࠋࡉࡽ࡟㸪ᮏ⑓↓Ⓨ⏕ᆅ༊࡛ࡶ㸪✺Ⓨⓗ࡞⑓ཎ⳦ࡢ౵ධ࡟
ࡼࡾ㸪ⰼ⢊ࢆởᰁࡉࡏ࡚ࡋࡲ࠺ࣜࢫࢡࡀ࠶ࡿࡓࡵ㸪ࡑࡢࣜࢫࢡࢆపῶࡉࡏࡿⰼ⢊ࡢᾘẘἲࡢ᳨ウࡀᚲせ࡛࠶ࢁ࠺ࠋㅰ ㎡ᮏ◊✲ࢆ⾜࠺࡟࠶ࡓࡾ㸪⌧ᆅࡢ -$ ࠾ࡼࡧ⏕⏘⪅㸪࡞ࡽࡧ࡟ᬑཬᣦᑟࢭࣥࢱ࣮࡟ࡣⰼ⢊ࡢ཰㞟࠾ࡼࡧⓎ⏕≧ἣㄪᰝ࡟༠ຊࡋ࡚࠸ࡓࡔ࠸ࡓࠋࡲࡓ㸪⚟ᒸࡢ࢟࢘࢖ࣇ࣮ࣝ
ࢶࠕ⏑࠺࠷ࠖᬑཬ᥎㐍༠㆟఍࡟ࡣ᳨ᰝࡢእ㒊ጤク࡟༠ຊࡋ࡚࠸ࡓࡔ࠸ࡓࠋ⑓ᐖ⹸㒊㛗ᯇᮏᖾᏊẶ࠾ࡼࡧࢳ࣮࣒㛗ΎỈಙᏕẶ࡟ࡣຓゝࢆ㡬࠸ࡓࠋ῝ㅰࡢពࢆ⾲ࡍࡿࠋᘬ⏝ᩥ⊩
&XQW\ $ 3ROLDNRII ) 5LYRDO & &HVEURQ 6 6DX[0)/ /HPDLUH & -DFTXHV 0$ 0DQFHDX &
9DQQHVWH -/   &KDUDFWHUL]DWLRQ RI
3VHXGRPRQDV V\ULQJDH SY DFWLQLGLDH 3VD 
LVRODWHG IURP )UDQFH DQG DVVLJQPHQW RI 3VDELRYDU  WR D GH QRYR SDWKRYDU 3VHXGRPRQDVV\ULQJDH SY DFWLQLGLIROLRUXP SY QRY 3ODQW3DWKRO㸸–㸬
*DOOHOOL$7DORFFL6/ $8525$$/RUHWL6  'HWHFWLRQ RI 3VHXGRPRQDV V\ULQJDH SYDFWLQLGLDH FDXVDO DJHQW RI EDFWHULDO FDQNHU
RI NLZLIUXLW IURP V\PSWRPOHVV IUXLWV DQGWZLJVDQGIURPSROOHQ3K\WRSDWKRO0HGRWHUU㸸–㸬
⳥ཎ㈼ḟ࣭ᡂᒣ⚽ᶞ࣭ᅧ୸ㅬ஧࣭▼஭㈗᫂  ⚟ᒸ┴࡟ ࠾ ࡅ ࡿ  ᖺ ࡢ ࢟ ࢘ ࢖ ࣇ ࣝ ࣮ ࢶ ࠿ ࠸ ࡼ ࠺ ⑓
ELRYDU 3VD ࡢⓎ⏕≧ἣ㸬᪥᳜⑓ሗ 㸸–㸬
⳥ཎ㈼ḟ࣭ᡂᒣ⚽ᶞ࣭▼஭㈗᫂  ⚟ᒸ┴࡟࠾ࡅࡿ ᖺࡢ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓ ELRYDU 3VDࡢⓎ⏕≧ἣ㸬᪥᳜⑓ሗ 㸸–㸬
03, 0LQLVWU\IRUSULPDU\LQGXVWULHV   'HWHFWLRQ
RI3VHXGRPRQDVV\ULQJDHSYDFWLQLGLDHIURPOHDYHV
DQGSROOHQFROOHFWHGIURPV\PSWRPDWLFDQGDV\PSWRPDWLF
$FWLQLGLDFKLQHQVLVLQ7H3XNH%D\RI3OHQW\03,
$XFNODQGKWWSZZZNYKRUJQ]YGEGRFXPHQW &LWHG-XO\ ᡂᒣ⚽ᶞ࣭⳥ཎ㈼ḟ࣭ᅧ୸ㅬ஧࣭Ύ⏣┿஧ 㸧ᖺ᫓㹼ኟᏘ࡟࠾ࡅࡿ࢟࢘࢖ࣇ࣮ࣝࢶ࠿ࡽࡢ 3VHXGRPRQDVV\ULQJDHSYDFWLQLGLDHELRYDU 3VD ࡢ᳨ฟ㸬஑⑓⹸◊ሗ 㸸㸬㸦ㅮせ㸧
㎰ᯘỈ⏘┬㸦㸧➨㸱ᅇ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓ࡢ
3VD ⣔ ⤫ ࡟ 㛵 ࡍ ࡿ 㜵 㝖 ᑐ ⟇ ᑓ 㛛 ᐙ ఍ ㆟ ࡢ ᴫ
せ KWWSZZZPDIIJRMSMV\RXDQV\RNXERJDLF\X
VLU\RXRWKHUSGIKBJDL\RXSGI  ᖺ  ᭶ ᪥㜀ぴ ㎰ᯘỈ⏘┬ D 3VHXGRPRQDVV\ULQJDHSYDFWLQLGLDHELRYDU ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓⳦ 3VD ࡟㛵

10

福岡県農林業総合試験場研究報告 ５（2019）ࡍࡿ⑓ᐖ⹸ࣜࢫࢡ࢔ࢼࣜࢩࢫሗ࿌᭩㸬ᶓ὾᳜≀㜵␿ᡤㄪᰝ◊✲㒊ᶓ὾KWWSZZZPDIIJRMSM
V\RXDQNHQHNLNLNDNXSGIBBBSVHXGRPBV\ULQJDBSYBDFWLQLGBELRYDUBSUDSGI  ᖺ  ᭶  ᪥㜀ぴ ㎰ᯘỈ⏘┬ E ᳜≀㜵␿ἲ᪋⾜つ๎ ┬௧ ࡢ᪂ᪧᑐ↷᮲ᩥ㸬KWWSZZZPDIIJRMSMV\RXDQNHQHNLNLNDNXSGIV\RXUHLSGI  ᖺ  ᭶  ᪥㜀ぴ ㎰ᯘỈ⏘┬ D ᳜≀᳨␿⤫ィ᝟ሗ㸬KWWSZZZSSVJRMS7RNHL:::3DJHVWRXNHL/LVWWRXNHL,QIR/LVW[KWPO  ᖺ  ᭶  ᪥㜀ぴ ㎰ᯘỈ⏘┬ E ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓ࡢ 3VD ⣔⤫ࡢ㜵㝖࣐ࢽࣗ࢔ࣝ ➨  ∧ 㸬KWWSZZZPDIIJRMSMV\RXDQV\RNXERJDLF\XVLU\RXDWWDFKSGI
LQGH[SGI  ᖺ  ᭶  ᪥㜀ぴ ㎰㣗஦ᴗ & ࢥࣥࢯ࣮ࢩ࢔࣒㸦㸧3VD ⣔⤫࡟ࡼࡿ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓࡟ᑐࡍࡿ㜵㝖ᑐ⟇ᢏ⾡㸬
㎰◊ᶵᵓ㸪ᮾᗈᓥᕷ㸪S–㸬኱㇂⾨࣭∦ᒸ㑳㞝࣭⛅ග࿴ஓ  㤶ᕝ┴ෆ࡛ศ㞳ࡉࢀࡓ࢟࢘࢖࠿࠸ࡼ࠺⑓⳦ࡢࢤࣀ࣒ゎᯒ㸬ᖹᡂ  ᖺᗘ᪥ᮏ᳜≀⑓⌮Ꮫ఍኱఍ࣉࣟࢢ࣒࣭ࣛㅮ₇せ᪨ண✏㞟 S㸬
5HHV*HRUJH - 9DQQHVWH -/ &RUQLVK '$
3XVKSDUDMDK ,36 <X - 7HPSOHWRQ 0' (YHUHWW
.5  'HWHFWLRQRI 3VHXGRPRQDVV\ULQJDHSYDFWLQLGLDH XVLQJ SRO\PHUDVH FKDLQ UHDFWLRQ
3&5  SULPHUV EDVHG RQ WKH 66 U'1$
LQWHUWUDQVFULEHG VSDFHU UHJLRQ DQG FRPSDULVRQZLWK 3&5SULPHUV EDVHGRQ RWKHUJHQH UHJLRQV3ODQW3DWKRO㸸–㸬⃝ ⏣ ᏹ அ ࣭ ୕ ዲ Ꮥ ඾ ࣭ ஭ ᡭ ὒ ୍  3VHXGRPRQDVV\ULQJDH SY DFWLQLGLDH ࡢ ᪂ つ 0/6$ ࢢ ࣝ ࣮ ࣉ
3VD ⣔⤫ ࡟ࡼࡗ࡚ $FWLQLGLDFKLQHQVLV ࡟Ⓨ⏕ࡋࡓ࠿࠸ࡼ࠺⑓㸬᪥᳜⑓ሗ 㸸–㸬⃝⏣ᏹஅ࣭ΎỈఙ୍࣭୕ዲᏕ඾࣭⠛ᓮẎ࣭ᴋඖᬛᏊ࣭
㔝ཱྀ┿ᘪ࣭ᡂᐩẎㄅ࣭⳥ཎ㈼ḟ࣭㛫బྂᑗ๎࣭⸨ᕝ㈗ ྐ ࣭ ୰ ␇ Ⰻ ஧  ࢃ ࡀ ᅜ ࡛ ศ 㞳 ࡉ ࢀ ࡓ
3VHXGRPRQDVV\ULQJDHSY DFWLQLGLDHELRYDU ࡢ≉ᚩ㸬᪥᳜⑓ሗ 㸸–㸬⃝⏣ᏹஅ࣭㏆⸨㈼୍࣭୰␇Ⰻ஧  ࢟࢘࢖ࣇ࣮ࣝࢶ࠿
࠸ࡼ࠺⑓⳦ 3VHXGRPRQDVV\ULQJDHSYDFWLQLGLDH
ࡢ᪂つ ELRYDU ELRYDU ࡢ≉ᚩ㸬᪥᳜⑓ሗ 㸸–㸬ΎỈఙ୍࣭⃝⏣ᏹஅ࣭⠛ᓮẎ࣭ᴋඖᬛᏊ࣭㔝ཱྀ┿ᘪ࣭⳥ཎ㈼ḟ࣭୕ዲᏕ඾  ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺
⑓⳦ࡢ ELRYDU ࢆ᳨ฟࡍࡿ᪂ࡋ࠸ࣉࣛ࢖࣐࣮ࡢస〇᪥᳜⑓ሗ 㸸㸬⠛ᓮẎ࣭ᴋඖᬛᏊ࣭ΎỈఙ୍࣭୰ᕝ㞞அ  ᤵ⢊࡟ࡼࡿ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓⳦ࡢឤᰁࡢྍ⬟ᛶ㸬᪥᳜⑓ሗ 㸸㸬
6WHIDQL ( *LRYDQDUGL '   'LVVHPLQDWLRQ RI
3VHXGRPRQDV V\ULQJDH SY DFWLQLGLDH WKURXJKSROOHQ DQG LWV HSLSK\WLF OLIH RQ OHDYHV DQGIUXLWV3K\WRSDWKRL0HGLWHUU㸸–㸬
7DNLNDZD < 6HUL]DZD 6 ,FKLNDZD 7 7VX\XPX 6
*RWR 0   3VHXGRPRQDV V\ULQJDH SYDFWLQLGLDH SY QRY WKH FDXVDO EDFWHULXP RI
FDQNHU RI NLZLIUXLW LQ -DSDQ $QQ 3K\WRSDWK
6RF-DSDQ㸸–㸬
7RQWRX5*LRYDQDUGL'6WHIDQL(  3ROOHQDV
D SRVVLEOH SDWKZD\ IRU WKH GLVVHPLQDWLRQ RI
3VHXGRPRQDV V\ULQJDH SY DFWLQLGLDH DQG EDFWHULDOFDQNHURINLZLIUXLW3K\WRSDWKRORJLD0HGLWUUDQHD㸸–㸬∵ᒣḯྖ  ࢟࢘࢖ࣇ࣮ࣝࢶ࠿࠸ࡼ࠺⑓ࡢ⏕ែ࡜㜵㝖࡟㛵ࡍࡿ◊✲㸬⚄ዉᕝ┴ᅬⱁヨ㦂ሙ◊✲ሗ࿌㸸–㸬