Study tour to the bean sprout factory, Yukiguni Maitake Co. Ltd. Minamiuonuma, Niigata, Japan

Opening Remarks
Dr. H. Hirochika

Glycine Genetic Resources
Dr. D.A. Vaughan

Vigna Genetic Resources
Dr. N. Tomooka

Glycine Genomics
Dr. K. Harada

Vigna Genomics
Dr. A. Kaga

Vigna Transformation
Dr. P.K. Jaiwal

Production & Breeding(Japan)Dr. M. Hajika

Production & Breeding(India)Dr. M. Pandiyan

Production & Breeding(Thailand)
Dr. P. Srinives

Production & Breeding(Lao PDR)
Ms. K. Kanyavong

Production & Breeding(Indonesia)
Dr. R. Soehendi

Production & Breeding(China)
Prof. C. Xuzhen

Production & Breeding(China)Ms. T. Jing

Closing Remarks
Dr. M. Kawase

Hokkaido SatelliteSymposium,Opening Remarks,
Mr. Hanaoka

Production & Breeding(Hokkaido, Japan)
Mr. H. Shimada

Soybean Flowering
Dr. J. AbeAzuki Bean Diseases
Dr. N. Kondo

Azuki Bean NematodeMr. S. Aoyama

Hokkaido Satellite Symposium at Tokachi Agricultural Experiment Station. In front ot the Seed Monument of “Erimo-shozu”,
the most popular azuki bean cultivar released from this station.

Study tour to the “Sapporo Beer Factory”, Eniwa, Hokkaido, Japan

The 14th
National Institute of Agrobiological Siences, Japan(NIAS)
International Workshop on Genetic Resources

Genetic Resources and Comparative Genomicsof Legumes (Glycine and Vigna)

September 14, 2009

Sponsored by
National Institute of Agrobiological Sciences

Published November 2011
ISBN 978-4-931511-22-4

Welcome Address
Hirochika Hirohiko
Director, Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences

I would like to welcome all participants to this workshop. I am particularly happy to welcome friends andcollaborators who have made long journeys from overseas.
Recently in the press there are repeated warnings that global food security is threatened by various factors
including global climate changes and increasing population. FAO predicts that Asia will lack the means tofeed the extra 1.5 billion people that will live on our continent by 2050.
Of human foods there are two groups that are most important - cereals and legumes. Research progressin understanding the main cereal of Asia, rice, has established this crop as a model for all crops. Legumes
have received less attention than the cereals until recently, but now there are a number of new research
initiatives to address legume crops.
Today this workshop will focus on two major and closely related legume genera - Vigna and Glycine . The

Vigna includes many important cultivated legumes including cowpea, mungbean and azuki bean. Vigna
are the major legumes of South Asia and Africa. Glycine is the genus of soybean. Soybean is the mostimportant legume globally. In Japan there are abundant genetic resources of both these genera and Japan isa center of diversity for soybean and wild soybean as well as azuki bean and wild azuki bean.
The National Institute of Agrobiological Science Genebank has taken a leadership role in collecting,conserving and conducting research on genetic resources of Japanese Vigna and Glycine . Through ourcollaborators, many of the overseas participants here today, we have also been collecting and conserving
these genera from other countries. These genetic resources are invaluable resources for breeding andgenomics research.
Recently NIAS embarked on a soybean genomics project and this is complemented by the pioneering

Vigna genomics research also being conducted in NIAS. Our soybean genomics initiative is part of a global
effort to understand the genome of soybean so that more progress can be achieved in soybean breeding.
The soybean genome is a distinct challenge because of its size but fortunately developments in sequencing
and information management mean that rapid progress is being made and some of that progress will bereported today.
The meeting today will provide an opportunity to discuss what is being done and also what needs tobe done in different countries to make progress in conservation, evaluation and use of legume genetic
resources and also legume genomics. I hope that by the end of the day you will all have ideas that will
benefit your future work. In addition, for those that are going for the satellite workshop in Hokkaido, I hope
today ‘breaks the ice’ and enables you to develop new linkages with colleagues in different countries.

Contents
Glycine Genetic Resources
Vaughan D.A., N. Tomooka, A. Kaga, T. Isemura and Y. Kuroda

1

Vigna Genetic Resources
Tomooka N., A. Kaga, T. Isemura, Y. Kuroda, D.A. Vaughan, P. Srinives, P. Somta,S. Thadavong, C. Bounphanousay, K. Kanyavong, P. Inthapanya, M. Pandiyan, N. Senthil,N. Ramamoorthi, P.K. Jaiwal, T. Jing, K. Umezawa and T. Yokoyama

11

Genetic and Molecular Analysis of Soybean Genome

Harada, K., A. Kaga, Y. Katayose, Y. Tsubokura, S. Sato, S. Watanabe, Z. Xia,
M. Hayashi, H. Kanamori, T. Shimizu, K. Machita, H. Ikawa, T. Ito, K. Kurita, K. Ito,
J. Wu, T. Matsumoto, S. Tabata and T. Sasaki

23

Asian Vigna Genome ResearchKaga A., T. Isemura, T. Shimizu, P. Somta, P. Srinives, S.Tabata, N. Tomookaand D.A. Vaughan

33

Genetic Transformation of Vigna Species: Current Status and Future Prospectives

Chaudhary D., M. Sainger, L. Sahoo and P.K. Jaiwal

41

Present State and Prospect of Soybean Production and Soybean Breeding in Japan

Hajika M.

49

Present Status and Future Perspectives of Glycine and Vigna in India

Pandiyan M., N. Senthil, N. Ramamoorthi and S.K. Ganesh

53

Interspecific Hybridization of Vigna radiata x 13 wild Vigna Species

Pandiyan M., N. Senthil, N. Ramamoorthi and T. Jayaraj

61

Present Status and Future Perspectives of Glycine and Vigna in Thailand

Srineves P. and P. Somta

63

Status of Glycine and Vigna in the Lao PDR

Kanyavong K., C. Bounphanousay, P. Inthapanya and B. Kanyavong

69

Present Status and Future Perspectives of Soybean Production in Indonesia

Soehendi R.

77

Status and Future Perspectives of Vigna (Mungbean and Azuki bean) Production andResearch in China

Xuzhen C. and T. Jing

83

Azuki Bean Cultivation in Hokkaido and Breeding at Tokachi Agr. Exp. Station

Shimada H., A.Tazawa, and H.Sato87

Genetic Diversity and Its Use in Soybean

Abe J.

91

Brown Stem Rot and Phytophthora Stem Rot: Limiting Factors in Azuki Bean Production

Kondo N.

99

Breeding Soybean Cyst Nematode Resistant Azuki Bean using Wild Vigna Germplasm

Aoyama S. and A. Kushida103