Curriculum Vitae
Keiichi Inoue, Ph. D.
May 22nd, 2023

Address for correspondence
The Institute for Solid State Physics, The University of Tokyo
5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8581, Japan.
Telephone & Fax: +81-4-7136-3230
E-mail: inoue@issp.u-tokyo.ac.jp
Web: https://inoue.issp.u-tokyo.ac.jp/en/index.html
ORCID: 0000-0002-6898-4347
twitter: @LabInoue

Academic employment
April, 2018-present

Associate Professor, The Institute for Solid State Physics,
The University of Tokyo

April, 2016- March, 2018

Associate Professor, Nagoya Institute of Technology

December, 2015-March, 2019

PRESTO, JST researcher, Japan

October, 2012-November, 2015

PRESTO, JST researcher, Japan

November, 2009- March, 2016

Assistant Professor, Nagoya Institute of Technology

April, 2007- October, 2009

Project

Assistant

Professor,

ChemicalResources

Laboratory, Tokyo Institute of Technology
April, 2006-March, 2007

JSPS Fellow (DC2)

Academic Degrees
April, 2004-March, 2007

Ph. D.

(Science), Department of Chemistry, Kyoto

University
April, 2002-March, 2004

M. S. Department of Chemistry, Kyoto University

April, 1998-March, 2002

B. S. Department of Chemistry, Kobe University

Original papers (all peer-reviewed)(*: Corresponding authors, †: Equally contributed authors)

1.

A Light-Gated Cation Channel with High Reactivity to Weak Light
Shoko Hososhima, Shinji Ueno, Satoshi Okado, Ken-ichi Inoue, Masae Konno, Yumeka

Yamauchi, Keiichi Inoue, Hiroko Terasaki, Hideki Kandori*, Satoshi P. Tsunoda*. (2023)
Scientific Reports, 13, Article number: 7675.
doi: 10.1038/s41598-023-34687-7

2.

Twisting and Protonation of Retinal Chromophore Regulates Channel Gating ofChannelrhodopsin C1C2
Keisei Shibata, Kazumasa Oda, Tomohiro Nishizawa, Yuji Hazama, Ryohei Ono, Shunki
Takaramoto, Reza Bagherzadeh, Hiromu Yawo, Osamu Nureki, Keiichi Inoue*, Hidefumi
Akiyama*. (2023) Journal of the American Chemical Society, published on the web.
doi: 10.1021/jacs.3c01879s

3.

Converting a Natural Light-driven Outward Proton Pump Rhodopsin into an
Artificial Inward Proton Pump
María del Carmen Marín, Masae Konno, Hiromu Yawo, Keiichi Inoue*. (2023) Journal of theAmerican Chemical Society, published on the web.
doi: 10.1021/jacs.2c12602

4.

Time-Resolved Detection
HeliorhodopsinofLight-induced

Conformational

Changesof

Yusuke Nakasone, Yuma Kawasaki, Masae Konno, Keiichi Inoue, Masahide Terazima*. (2023)
Physical Chemistry Chemical Physics, 25, Issue 18, pp 12833-12840.
doi: 10.1039/D3CP00711A

5.

Reversible Photoreaction of a Retinal Photoisomerase, Retinal G Protein-Coupled
Receptor RGR
Naoya Morimoto†, Takashi Nagata†, Keiichi Inoue*. (2023) Biochemistry, 62, Issue 9, pp 14291432.
doi: 10.1021/acs.biochem.3c00084

6.

Difference FTIR Spectroscopy of Jumping Spider Rhodopsin-1 at 77 K
Shunpei Hanai, Takashi Nagata, Kota Katayama, Shino Inukai, Mitsumasa Koyanagi, Keiichi
Inoue, Akihisa Terakita, Hideki Kandori*. (2023) Biochemistry, 62, Issue 8, pp 1347-1359.
doi: 10.1021/acs.biochem.3c00022

7.

Biophysical Characterization of Microbial Rhodopsins with DSE Motif
María del Carmen Marín†, Alexander L. Jaffe†, Patrick T. West, Masae Konno, Jillian F.
Banfield*, Keiichi Inoue*. (2023) Biophysics and Physicobiology, 20, Special Issue, Article
number: e201023.
doi: 10.2142/biophysico.bppb-v20.s023

8.

Characterization of Retinal Chromophore and Protonated Schiff Base in
Thermoplasmatales Archaeon Heliorhodopsin Using Solid-State NMR
Spectroscopy

Shibuki Suzuki†, Sari Kumagai†, Toshio Nagashima, Toshio Yamazaki, Takashi Okitsu, Akimori
Wada, Akira Naito, Kota Katayama, Keiichi Inoue, Hideki Kandori, Izuru Kawamura*. (2023)
Biophysical Chemistry, 296, Article number: 106991.
doi: 10.1016/j.bpc.2023.106991

9.

Protein Dynamics of a Light-Driven Na+ Pump Rhodopsin Probed Using a
Tryptophan Residue Near the Retinal Chromophore
Akihiro Otomo, Misao Mizuno, Keiichi Inoue, Hideki Kandori, Yasuhisa Mizutani*. (2023)
Biophysics and Physicobiology, 20, Special Issue, Article number: e201016.
doi: 10.2142/biophysico.bppb-v20.s016

10. Phototrophy by Antenna-Containing Rhodopsin Pumps in Aquatic Environments
Ariel Chazan†, Ishita Das†, Takayoshi Fujiwara†, Shunya Murakoshi†, Andrey Rozenberg, Ana
Molina-Marquez, Fumiya K. Sano, Tatsuki Tanaka, Patricia Gomez-Villegas, Shirley Larom,
Alina Pushkarev, Partha Malakar, Masumi Hasegawa, Yuya Tsukamoto, Tomohiro Ishizuka,
Masae Konno, Takashi Nagata, Yosuke Mizuno, Kota Katayama, Rei Abe-Yoshizumi, Sanford
Ruhman, Keiichi Inoue, Hideki Kandori, Rosa M. Leon, Wataru Shihoya*, Susumu Yoshizawa*,
Mordechai Sheves*, Osamu Nureki*, Oded Béjà*. (2023) Nature, 615, Issue 7952, pp 535-540
doi: 10.1038/s41586-023-05774-6

11. Proton-transporting Heliorhodopsins from Marine Giant Viruses
Shoko Hososhima, Ritsu Mizutori, Rei Abe-Yoshizumi, Andrey Rozenberg, Shunta Shigemura,
Alina Pushkarev, Masae Konno, Kota Katayama, Keiichi Inoue, Satoshi P. Tsunoda, Oded Béjà,
Hideki Kandori*. (2022) eLife, 11, Article number: e78416
doi: 10.7554/eLife.78416

12. Kinetic Study on The Molecular Mechanism of Light-driven Inward Proton
Transport by Schizorhodopsins
Yuma Kawasaki, Masae Konno, Keiichi Inoue*. (2022) Biochimica et Biophysica Acta (BBA) Biomembranes, 1864, issue 11, Article number: 184016
doi: 10.1016/j.bbamem.2022.184016

13. Rhodopsin-bestrophin Fusion Proteins from Unicellular Algae form Gigantic
Pentameric Ion Channels
Andrey Rozenberg†, Igor Kaczmarczyk†, Donna Matzov†, Johannes Vierock†, Takashi Nagata,
Masahiro Sugiura, Kota Katayama, Yuma Kawasaki, Masae Konno, Yujiro Nagasaka, Mako
Aoyama, Ishita Das, Efrat Pahima, Jonathan Church, Suliman Adam, Veniamin A. Borin, Ariel
Chazan, Sandra Augustin, Jonas Wietek, Julien Dine, Yoav Peleg, Akira Kawanabe, Yuichiro
Fujiwara, Ofer Yizhar, Mordechai Sheves, Igor Schapiro, Yuji Furutani, Hideki Kandori, Keiichi
Inoue, Peter Hegemann, Oded Béjà*, Moran Shalev-Benami* (2022) Nature Structural &Molecular Biology, 29, No. 6, pp 592-603

doi: 10.1038/s41594-022-00783-x

14. Saccharibacteria Harness Light Energy Using Type-1 Rhodopsins That May Rely on
Retinal Sourced from Microbial Hosts
Alexander L. Jaffe†, Masae Konno†, Yuma Kawasaki, Chihiro Kataoka, Oded Béjà, Hideki
Kandori, Keiichi Inoue*, Jillian F. Banfield* (2022) The ISME Journal, 16, issue 3, pp 20562059
doi: 10.1038/s41396-022-01231-w

15. Structural Characterization of Proton-pumping Rhodopsin Lacking a Cytoplasmic
Proton Donor Residue by X-ray Crystallography
Kano Suzuki†, María del Carmen Marín†, Masae Konno†, Reza Bagherzadeh, Takeshi Murata*,
Keiichi Inoue* (2022) Journal of Biological Chemistry, 298, No. 8, pp 101722
doi: 10.1016/j.jbc.2022.101722

16. Structural Basis for Channel Conduction in the Pump-like Channelrhodopsin
ChRmineKoichiro E. Kishi†, Yoon Seok Kim†, Masahiro Fukuda†, Tsukasa Kusakizako†, Elina Thadhani,
Eamon F.X. Byrne, Joseph M. Paggi, Charu Ramakrishnan, Toshiki E. Matsui, Keitaro
Yamashita, Takashi Nagata, Masae Konno, Peter Y. Wang, Masatoshi Inoue, Tyler Benster,
Tomoko Uemura, Kehong Liu, Mikihiro Shibata, Norimichi Nomura, So Iwata, Osamu Nureki,
Ron O. Dror, Keiichi Inoue, Karl Deisseroth*, Hideaki E. Kato* (2022) Cell, 185, issue 4, pp
672-689.e23
doi: 10.1016/j.cell.2022.01.007

17. Diverse Heliorhodopsins Detected via Functional Metagenomics in Freshwater
Actinobacteria, Chloroflexi and Archaea
Ariel Chazan†, Andrey Rozenberg†, Kentaro Mannen, Takashi Nagata, Ran Tahan, Shir Yaish,
Shirley Larom, Keiichi Inoue, Oded Béjà, Alina Pushkarev* (2022) Environmental
Microbiology, 24, issue 1, pp 110-121
doi: 10.1111/1462-2920.15890

18. Heliorhodopsin Evolution is Driven by Photosensory Promiscuity in Monoderms
Paul-Adrian Bulzu, Vinicius Silva Kavagutti, Maria-Cecilia Chiriac, Charlotte D. Vavourakis,
Keiichi Inoue, Hideki Kandori, Adrian-Ştefan Andrei, Rohit Ghai* (2021) mSphere, 6, No. 6,
Article number: e00661-21
doi: 10.1128/mSphere.00661-21

19. Pro219 is an Electrostatic Color Determinant in the Light-driven Sodium Pump KR2
Yuta Nakajima†, Laura Pedraza-González†, Leonardo Barneschi, Keiichi Inoue, Massimo
Olivucci*, Hideki Kandori* (2021) Communications Biology, 4, Article number: 1185
doi: 10.1038/s42003-021-02684-z

20. Thermostable Light-Driven Inward Proton Pump Rhodopsins
Yuma Kawasaki, Masae Konno, Keiichi Inoue*. (2021) Chemical Physics Letters, 779,
Article number: 138868
doi: 10.1016/j.cplett.2021.138868

21. Crystal Structure of Schizorhodopsin Reveals Mechanism of Inward Proton
PumpingAkimitsu Higuchi†, Wataru Shihoya†,*, Masae Konno, Tatsuya Ikuta, Hideki Kandori, Keiichi
Inoue*, Osamu Nureki*. (2021) Proceedings of the National Academy of Sciences of the UnitedStates of America, 118, No. 14, Article number: e2016328118
doi: 10.1073/pnas.2016328118

22. Time-Resolved Serial Femtosecond Crystallography Reveals Early Structural
Changes in Channelrhodopsin
Kazumasa Oda†, Takashi Nomura†, Takanori Nakane, Keiichi Inoue, Shota Ito, Johannes
Vierock, Kunio Hirata, Keitaro Yamashita, Andrés D. Maturana, Tatsuya Ikuta, Itsuki Ishigami,
Tamaki Izume, Rie Umeda, Ryuun Eguma, Satomi Oishi, Go Kasuya, Takafumi Kato, Tsukasa
Kusakizako, Wataru Shihoya, Hiroto Shimada, Tomoyuki Takatsuji, Mizuki Takemoto, Reiya
Taniguchi, Atsuhiro Tomita, Ryouki Nakamura, Masahiro Fukuda, Hirotake Miyauchi,
Yongchan Lee, Eriko Nango, Rie Tanaka, Tomoyuki Tanaka, Michihiro Sugawara, Tetsunari
Kimura, Tatsuro Shimamura, Takaaki Fujiwara, Yasuaki Yamanaka, Shigeki Owada, Yasumasa
Joti, Kensuke Tono, Ryuichiro Ishitani, Shigehiko Hayashi, Hideki Kandori, Peter Hegemann,
So Iwata, Minoru Kubo*, Tomohiro Nishizawa*, Osamu Nureki*. (2021) eLife, 10, Article
number: e62389
doi: 10.7554/eLife.62389

23. Exploration of Natural Red-shifted Rhodopsins Using a Machine Learning-based
Bayesian Experimental Design
Keiichi Inoue†,*, Masayuki Karasuyama†, Ryoko Nakamura, Masae Konno, Daichi Yamada,
Kentaro Mannen, Takashi Nagata, Yu Inatsu, Hiromu Yawo, Kei Yura, Oded Béjà, Hideki
Kandori, Ichiro Takeuchi*. (2021) Communications Biology, 4, issue 1, Article number: 362
doi: 10.1038/s42003-021-01878-9

24. TAT Rhodopsin is a UV-Dependent Environmental pH Sensor
Chihiro Kataoka, Teppei Sugimoto, Shunta Shigemura, Kota Katayama, Satoshi P. Tsunoda,
Keiichi Inoue, Oded Béjà, Hideki Kandori*. (2021) Biochemistry, 60, issue 12, pp 899-907
doi: 10.1021/acs.biochem.0c00951

25. Expression Analysis of Microbial Rhodopsin-like Genes in Guillardia theta
Masae Konno*, Yumeka Yamauchi, Keiichi Inoue, Hideki Kandori*. (2020) PLOS ONE, 15,
issue 12, Article number: e0243387

doi: 10.1371/journal.pone.0243387

26. Active Learning for Level Set Estimation Under Input Uncertainty and Its
Extensions
Yu Inatsu, Masayuki Karasuyama, Keiichi Inoue, Ichiro Takeuchi*. (2020) Neural Computation,
32, issue 12, pp 2486-2531
doi: 10.1162/neco_a_01332

27. Active Learning of Bayesian Linear Models with High-Dimensional Binary Features
by Parameter Confidence-Region Estimation
Yu Inatsu, Masayuki Karasuyama, Keiichi Inoue, Hideki Kandori, Ichiro Takeuchi*. (2020)
Neural Computation, 32, issue 10, pp 1998-2031
doi: 10.1162/neco_a_01310

28. Excitonic Coupling Effect on the Circular Dichroism Spectrum of Sodium-Pumping
Rhodopsin KR2
Kazuhiro J. Fujimoto*, Keiichi Inoue. (2020) The Journal of Chemical Physics, 153, Article
number: 045101
doi: 10.1063/5.0013642

29. Gate-Keeper of Ion Transport – a Highly Conserved Helix-3 Tryptophan in a
Channelrhodopsin Chimera, C1C2/ChRWR
Yujiro Nagasaka†, Shoko Hososhima†, Naoko Kubo, Takashi Nagata, Hideki
Kandori, Keiichi Inoue, Hiromu Yawo*. (2020) Biophysics and Physicobiology, 17,
pp. 59-70
doi: 10.2142/biophysico.BSJ-2020007

30. Schizorhodopsins: A Novel Family of Rhodopsins from Asgard archaea that
Function as Light-Driven Inward H+ Pumps
Keiichi Inoue*, Satoshi P. Tsunoda, Manish Singh, Sahoko Tomida, Shoko Hososhima, Masae
Konno, Ryoko Nakamura, Hiroki Watanabe, Paul-Adrian Bulzu, Horia L. Banciu, Adrian-Ştefan
Andrei, Takayuki Uchihashi, Rohit Ghai, Oded Béjà, Hideki Kandori*. (2020) Science Advances,
6, No. 15, Article number: eaaz2441
doi: 10.1126/sciadv.aaz2441

31. Infrared spectroscopic analysis on structural changes around the protonated Schiffbase upon retinal isomerization in light-driven sodium pump KR2
Sahoko Tomida, Shota Ito, Tomoya Mato, Yuji Furutani, Keiichi Inoue, Hideki Kandori*. (2020)
Biochimica et Biophysica Acta (BBA) - Bioenergetics, published on the web

doi: 10.1016/j.bbabio.2020.148190

32. Allosteric Communication to the Retinal Chromophore upon Ion Binding in a Lightdriven Sodium Ion Pumping Rhodopsin

Akihiro Otomo, Misao Mizuno, Keiichi Inoue, Hideki Kandori, Yasuhisa Mizutani*. (2019)
Biochemistry, 59, issue 4, pp 520-529
doi: 10.1021/acs.biochem.9b01062

33. Crystal Structure of Heliorhodopsin
Wataru Shihoya, Keiichi Inoue, Manish Singh, Masae Konno, Shoko Hososhima, Keitaro
Yamashita, Kento Ikeda, Akimitsu Higuchi, Tamaki Izume, Sae Okazaki, Masanori Hashimoto,
Ritsu Mizutori, Sahoko Tomida, Yumeka Yamauchi, Rei Abe-Yoshizumi, Kota Katayama,
Satoshi P. Tsunoda, Mikihiro Shibata, Yuji Furutani, Alina Pushkarev, Oded Béjà, Takayuki
Uchihashi, Hideki Kandori*, Osamu Nureki*. (2019) Nature, 574, issue 7776, pp 132-136
doi: 10.1038/s41586-019-1604-6

34. Unique Photochemistry Observed in a New Microbial Rhodopsin
Chihiro Kataoka, Keiichi Inoue, Kota Katayama, Oded Béjà, Hideki Kandori*. (2019) The
Journal of Physical Chemistry Letters, 10, issue 17, pp 5117-5121
doi: 10.1021/acs.jpclett.9b01957

35. X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin
Takefumi Morizumi†, Wei-Lin Ou†, Ned Van Eps, Keiichi Inoue, Hideki Kandori, Leonid S.
Brown, Oliver P. Ernst*. (2019) Scientific Reports, 9, 11283
doi: 10.1038/s41598-019-47445-5

36. Engineered Functional Recovery of Microbial Rhodopsin without Retinal-Binding
LysineYumeka Yamauchi, Masae Konno, Daichi Yamada, Kei Yura, Keiichi Inoue, Oded Béjà, Hideki
Kandori*. (2019) Photochemistry and Photobiology, 95, pp 1116-1121
doi: 10.1111/php.13114

37. Red-shifting Mutation of Light-driven Sodium Pump Rhodopsin
Keiichi Inoue, María del Carmen Marín, Sahoko Tomida, Ryoko Nakamura, Yuta Nakajima,
Massimo Olivucci, Hideki Kandori*. (2019) Nature Communications, 10, Article number: 1993
doi: 10.1038/s41467-019-10000-x

38. Casting Light on Asgardarchaeota Metabolism in a Sunlit Microoxic Niche
Paul-Adrian Bulzu†, Adrian-Ştefan Andrei†, Michaela M. Salcher, Maliheh Mehrshad, Keiichi
Inoue, Hideki Kandori, Oded Béjà, Rohit Ghai*, Horia L. Banciu. (2019) Nature Microbiology,
4, issue 7, pp 1129-1137
doi: 10.1038/s41564-019-0404-y

39. Ultrafast Dynamics of Heliorhodopsins
Shinya Tahara, Manish Singh, Hikaru Kuramochi, Wataru Shihoya, Keiichi Inoue, Osamu
Nureki, Oded Béjà, Yasuhisa Mizutani, Hideki Kandori, Tahei Tahara*. (2019) The Journal of
Physical Chemistry B, 123, issue 11, pp 2507-2512

doi: 10.1021/acs.jpcb.9b00887

40. Heliorhodopsins are Absent in Diderm (Gram-negative) Bacteria: Some Thoughts
and Possible Implications for Activity
José Flores-Uribe†, Gur Hevroni†, Rohit Ghai, Alina Pushkarev, Keiichi Inoue, Hideki Kandori,
Oded Béjà*. (2019) Environmental Microbiology Reports, 11, issue 3, pp 419-424
doi: 10.1111/1758-2229.12730

41. Resonance Raman Investigation of the Chromophore Structure of Heliorhodopsins
Akihiro Otomo, Misao Mizuno, Manish Singh, Wataru Shihoya, Keiichi Inoue, Osamu Nureki,
Oded Béjà, Hideki Kandori, Yasuhisa Mizutani*. (2018) The Journal of Physical Chemistry
Letters, 9, issue 22, pp 6431-6436
doi: 10.1021/acs.jpclett.8b02741

42. Understanding Colour Tuning Rules and Predicting Absorption Wavelengths ofMicrobial Rhodopsins by Data-Driven Machine-Learning Approach
Masayuki Karasuyama†, Keiichi Inoue†, Ryoko Nakamura, Hideki Kandori*, Ichiro Takeuchi*.
(2018) Scientific Reports, 8, Article number:15580
doi: 10.1038/s41598-018-33984-w

43. Crystal Structure of a Natural Anion-conducting Channelrhodopsin GtACR1
Yoon Seok Kim†, Hideaki E. Kato†,*, Keitaro Yamashita, Shota Ito, Keiichi Inoue, Charu
Ramakrishnan, Lief E. Fenno, Kathryn E. Evans, Joseph M. Paggi, Ron O. Dror, Hideki Kandori,
Brian K. Kobilka, Karl Deisseroth*. (2018) Nature, 561, issue 7723, pp 343-348
doi: 10.1038/s41586-018-0511-6

44. Structural Mechanisms of Selectivity and Gating in Anion Channelrhodopsins
Hideaki E. Kato†,*, Yoon Seok Kim†, Joseph M. Paggi, Kathryn E. Evans, William E. Allen,
Claire Richardson, Keiichi Inoue, Shota Ito, Charu Ramakrishnan, Lief E. Fenno, Keitaro
Yamashita, Daniel Hilger, Soo Yeun Lee, Andre Berndt, Kang Shen, Hideki Kandori, Ron O.
Dror, Brian K. Kobilka, Karl Deisseroth* (2018) Nature, 561, issue 7723, pp 349-354
doi: 10.1038/s41586-018-0504-5

45. Mutation Study of Heliorhodopsin 48C12
Manish Singh, Keiichi Inoue, Alina Pushkarev, Oded Béjà, Hideki Kandori*. (2018)
Biochemistry, 57, issue 33, pp 5041-5049
doi: 10.1021/acs.biochem.8b00637

46. Time-Resolved FTIR Study of Light-Driven Sodium Pump Rhodopsins
Hui-Fen Chen, Keiichi Inoue, Hikaru Ono, Rei Abe-Yoshizumi, Akimori Wada, Hideki
Kandori*. (2018) Physical Chemistry Chemical Physics, 20, issue 26, pp 17694-17704
doi: 10.1039/C8CP02599A

47. A Distinct Abundant Group of Microbial Rhodopsins Discovered via Functional

Metagenomics
Alina Pushkarev†, Keiichi Inoue†, Shirley Larom, José Flores-Uribe, Manish Singh, Masae
Konno, Sahoko Tomida, Shota Ito, Ryoko Nakamura, Satoshi P. Tsunoda, Alon Philosof, Itai
Sharon, Natalya Yutin, Eugene V. Koonin, Hideki Kandori*, Oded Béjà*. (2018) Nature, 558,
issue 7711, pp 595-599
doi: 10.1038/s41586-018-0225-9

48. Spectroscopic Study of Proton Transfer Mechanism of Inward Proton Pump
Rhodopsin, Parvularcula oceani Xenorhodopsin
Keiichi Inoue, Shinya Tahara, Yoshitaka Kato, Satoshi Takeuchi, Tahei Tahara*, Hideki
Kandori*. (2018) The Journal of Physical Chemistry B, 122, issue 25, pp 6453-6461
doi: 10.1021/acs.jpcb.8b01279

49. Hydrogen-bonding Network at the Cytoplasmic Region of A Light-driven Sodium
Pump Rhodopsin KR2
Sahoko Tomida, Shota Ito, Keiichi Inoue, Hideki Kandori*. (2018) Biochimica et Biophysica
Acta (BBA) - Bioenergetics, 1859, issue 9, pp 684-691
doi: 10.1016/j.bbabio.2018.05.017

50. Oligomeric States of Microbial Rhodopsins Determined by High-speed Atomic
Force Microscopy and Circular Dichroic Spectroscopy
Mikihiro Shibata†, Keiichi Inoue†, Kento Ikeda, Masae Konno, Manish Singh, Chihiro Kataoka,
Rei Abe-Yoshizumi, Hideki Kandori*, Takayuki Uchihashi*. (2018) Scientific Reports, 8, Article
number:8262
doi: 10.1038/s41598-018-26606-y

51. Origin of the Reactive and Non-Reactive Excited States in the Primary Reaction of
Rhodopsins: pH Dependence of Femtosecond Absorption of Light-Driven Sodium
Ion Pump Rhodopsin KR2
Shinya Tahara, Satoshi Takeuchi, Rei Abe-Yoshizumi, Keiichi Inoue, Hiroyuki Ohtani, Hideki
Kandori, Tahei Tahara*. (2018) The Journal of Physical Chemistry B, 122, issue 18, pp 47844792

doi: 10.1021/acs.jpcb.8b01934

52. Long-distance Perturbation on Schiff Base-counterion Interaction by His30 and The
Extracellular Na+-binding Site in Krokinobacter Rhodopsin 2
Arisu Shigeta, Shota Ito, Rina Kaneko, Sahoko Tomida, Keiichi Inoue, Hideki Kandori*, Izuru
Kawamura*. (2018) Physical Chemistry Chemical Physics, 20, issue 13, pp 8450-8455
doi: 10.1039/C8CP00626A

53. Effect of Temperature and Hydration Level on Purple Membrane Dynamics Studied
Using Broadband Dielectric Spectroscopy from Sub-GHz to THz Regions

Naoki Yamamoto, Shota Ito, Masahiro Nakanishi, Eri Chatani, Keiichi Inoue, Hideki Kandori,
Keisuke Tominaga*. (2018) The Journal of Physical Chemistry B, 122, issue 4, pp 1367-1377
doi: 10.1021/acs.jpcb.7b10077

54. Chimeric Microbial Rhodopsins for Optical Activation of Gs-proteins
Kazuho Yoshida, Takahiro Yamashita, Kengo Sasaki, Keiichi Inoue, Yoshinori Shichida, Hideki
Kandori*. (2017) Biophysics and Physicobiology, 14, pp 183-190
doi: 10.2142/biophysico.14.0_183

55. Unique Hydrogen Bonds in Membrane Protein Monitored by Whole Mid-IR ATR
Spectroscopy in Aqueous Solution
Shota Ito, Masayo Iwaki, Shinya Sugita, Rei Abe-Yoshizumi, Tatsuya Iwata, Keiichi Inoue,
Hideki Kandori*. (2017) The Journal of Physical Chemistry B, 122, issue 1, pp 165-170
doi: 10.1021/acs.jpcb.7b11064

56. Low-temperature FTIR Spectroscopy Evidences Protein-bound Water Molecules in
Eubacterial Light-driven Ion Pumps
Yurika Nomura, Shota Ito, Miwako Teranishi, Hikaru Ono, Keiichi Inoue, Hideki Kandori*.
(2017) Physical Chemistry Chemical Physics, 20, issue 5, pp 3165-3171
doi: 10.1039/c7cp05674e

57. Functional Characterization of Sodium-pumping Rhodopsins with Different
Pumping Properties
Satoshi P. Tsunoda, Matthias Prigge, Rei Abe-Yoshizumi, Keiichi Inoue, Yuko Kozaki, Toru
Ishizuka, Hiromu Yawo, Ofer Yizhar, Hideki Kandori*. (2017) PLOS ONE, 12, issue 7,
e0179232
doi: 10.1371/journal.pone.0179232

58. Molecular Properties of a DTD Channelrhodopsin from Guillardia theta
Yumeka Yamauchi, Masae Konno, Shota Ito, Satoshi P. Tsunoda, Keiichi Inoue, Hideki
Kandori*. (2017) Biophysics and Physicobiology, 14, pp 57-66
doi: 10.2142/biophysico.14.0_57

59. FTIR Analysis of a Light-driven Inward Proton Pumping Rhodopsin at 77 K
Shota Ito, Shinya Sugita, Keiichi Inoue, Hideki Kandori*. (2017) Photochemistry andPhotobiology, 93, issue 6, pp 1381-1387
doi: 10.1111/php.12771

60. Solid-state NMR Structural Study of the Retinal-binding Pocket in Sodium Ion Pump
Rhodopsin
Arisu Shigeta, Shota Ito, Keiichi Inoue, Takashi Okitsu, Akimori Wada, Hideki Kandori, Izuru
Kawamura*. (2017) Biochemistry, 56, issue 4, pp 543-550
doi: 10.1021/acs.biochem.6b00999

61. A Chimera Na+-pump Rhodopsin as an Effective Optogenetic Silencer
Mohammad Razuanul Hoque, Toru Ishizuka, Keiichi Inoue, Rei Abe-Yoshizumi, Hiroyuki
Igarashi, Takaaki Mishima, Hideki Kandori, Hiromu Yawo*. (2016) PLOS ONE, 11, issue 11,
e0166820
doi: 10.1371/journal.pone.0166820

62. Natural Light-driven Inward Proton Pump
Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi,
Satoshi P. Tsunoda, Hideki Kandori*. (2016) Nature Communications, 7, Article number:13415
doi: 10.1038/ncomms13415

63. Role of Asn112 in a Light-driven Sodium Ion-pumping Rhodopsin
Rei Abe-Yoshizumi, Keiichi Inoue, Hideaki E. Kato, Osamu Nureki, Hideki Kandori*. (2016)
Biochemistry, 55, issue 41, pp 5790-5797
doi: 10.1021/acs.biochem.6b00741

64. Primary Photocycle of Na+ Pump Rhodopsin Probed by Baseline-free Femto- to
Submillisecond Stimulated Raman Spectroscopy
Yusaku Hontani†, Keiichi Inoue†, Miroslav Kloz, Yoshitaka Kato, Hideki Kandori, John T. M.
Kennis*. (2016) Physical Chemistry Chemical Physics, 18, issue 35, pp 24729-24736
doi: 10.1039/C6CP05240A

65. Asymmetric Functional Conversion of Eubacterial Light-driven Ion Pumps
Keiichi Inoue, Yurika Nomura, Hideki Kandori*. (2016) The Journal of Biological Chemistry,
291, issue 19, pp 9883-9893 (Selected for “Highlights of 2016” in the Journal)
doi: 10.1074/jbc.M116.716498jbc.M116.716498.

66. Mutant of a Light-Driven Sodium Ion Pump Can Transport Cesium Ions
Masae Konno, Yoshitaka Kato, Hideaki E. Kato, Keiichi Inoue, Osamu Nureki, Hideki Kandori*.
(2016) The Journal of Physical Chemistry Letters, 7, issue 1, pp 51-55
doi: 10.1021/acs.jpclett.5b02385

67. Kinetic Analysis of H+ - Na+ Selectivity in a Light-Driven Na+ Pumping Rhodopsin
Yoshitaka Kato, Keiichi Inoue, Hideki Kandori*. (2015) The Journal of Physical Chemistry
Letters, 6, issue 24, pp 5111-5115
doi: 10.1021/acs.jpclett.5b02371

68. Ultrafast Photoreaction Dynamics of a Light-driven Sodium-ion-pumping Retinal
Protein from Krokinobacter eikastus Revealed by Femtosecond Time-resolved
Absorption Spectroscopy
Shinya Tahara, Satoshi Takeuchi, Rei Abe-Yoshizumi, Keiichi Inoue, Hiroyuki Ohtani, Hideki
Kandori*, Tahei Tahara*. (2015) The Journal of Physical Chemistry Letters, 6, issue 22, pp 44814486

doi: 10.1021/acs.jpclett.5b01994

69. A New Group of Eubacterial Light-driven Retinal-binding Proton Pumps with an
Unusual Cytoplasmic Proton Donor
Andrew Harris, Milena Ljumovic, Ana-Nicoleta Bondar, Yohei Shibata, Shota Ito, Keiichi Inoue,
Hideki Kandori*, Leonid S. Brown*. (2015) Biochimica et Biophysica Acta (BBA) Bioenergetics, 1847, issue 12, pp 1518-1529
doi: 10.1016/j.bbabio.2015.08.003

70. The Role of the NDQ-motif in Sodium Pump Rhodopsin
Keiichi Inoue, Masae Konno, Rei Abe-Yoshizumi, Hideki Kandori*. (2015) Angewandte
Chemie International Edition, 54, issue 39, pp 11536-11539
doi: 10.1002/anie.201504549

71. Structural Basis for Na+ Transport Mechanism by a Light-Driven Na+ Pump
Hideaki E. Kato, Keiichi Inoue, Rei Abe-Yoshizumi, Yoshitaka Kato, Hikaru Ono, Masae
Konno, Toru Ishizuka, Mohammad Razuanul Hoque, Shoko Hososhima, Hirohumi Kunitomo,
Jumpei Ito, Susumu Yoshizawa, Keitaro Yamashita, Mizuki Takemoto, Tomohiro Nishizawa,
Reiya Taniguchi, Kazuhiro Kogure, Andrés D. Maturana, Yuichi Iino, Hiromu Yawo, Ryuichiro
Ishitani, Hideki Kandori*, Osamu Nureki*. (2015) Nature, 521, Number 7550, 48–53
doi: 10.1038/nature14322

72. Converting a Light-driven Proton Pump into a Light-gated Proton Channel
Keiichi Inoue, Takashi Tsukamoto, Kazumi Shimono, Yuto Suzuki, Seiji Miyauchi, ShigehikoHayashi, Hideki Kandori, Yuki Sudo*. (2015) Journal of the American Chemical Society, 137,
issue 9, pp 3291-3299
doi: 10.1021/ja511788f

73. Na+ Transport by Sodium Ion Pump Rhodopsin is Resistant to Environmental
Change - A Comparison of Photocycles of Na+ and Li+ Transport Processes
Keiichi Inoue, Hikaru Ono, Hideki Kandori*. (2014) Chemistry Letters, 44, No. 3, 294-296
doi: 10.1246/cl.141023

74. Spectroscopic Study of a Light-Driven Chloride Ion Pump from Marine Bacteria
Keiichi Inoue, Faisal Hammad Mekky Koua, Yoshitaka Kato, Rei Abe-Yoshizumi, Hideki
Kandori*. (2014) The Journal of Physical Chemistry B, 118, issue 38, pp 11190-11199
doi: 10.1021/jp507219q

75. FTIR Spectroscopy of a Light-Driven Compatible Sodium Ion-Proton Pumping
Rhodopsin at 77 K
Hikaru Ono, Keiichi Inoue, Rei Abe-Yoshizumi, Hideki Kandori*. (2014) The Journal of
Physical Chemistry B, 118, issue 18, pp 4784-4792
doi: 10.1021/jp500756f

76. Chimeric Proton-Pumping Rhodopsins Containing the Cytoplasmic Loop of Bovine
Rhodopsin
Kengo Sasaki, Takahiro Yamashita, Kazuho Yoshida, Keiichi Inoue, Yoshinori Shichida, Hideki
Kandori*. (2014) PLoS ONE, 9, issue 3, e91323
doi: 10.1371/journal.pone.0091323

77. Role of Trimer-trimer Interaction of Bacteriorhodopsin Studied by Optical
Spectroscopy and High-speed Atomic Force Microscopy
Hayato Yamashita†, Keiichi Inoue†, Mikihiro Shibata, Takayuki Uchihashi, Jun Sasaki, Hideki
Kandori, Toshio Ando*. (2013) Journal of Structural Biology, 184, 2-11
doi: 10.1016/j.jsb.2013.02.011

78. Thermal and Spectroscopic Characterization of a Proton Pumping Rhodopsin froman Extreme Thermophile
Takashi Tsukamoto, Keiichi Inoue, Hideki Kandori, Yuki Sudo*. (2013) The Journal of
Biological Chemistry, 288, issue 30, pp 21581-21592
doi: 10.1074/jbc.M113.479394

79. A Blue-shifted Light-driven Proton Pump for Neural Silencing
Yuki Sudo*, Ayako Okazaki, Hikaru Ono, Jin Yagasaki, Seiya Sugo, Motoshi Kamiya, Louisa
Reissig, Keiichi Inoue, Kunio Ihara, Hideki Kandori, Shin Takagi, Shigehiko Hayashi. (2013)
The Journal of Biological Chemistry, 288, issue 28, pp 20624-20632
doi: 10.1074/jbc.M113

80. A Light-driven Sodium Ion Pump in Marine Bacteria
Keiichi Inoue, Hikaru Ono, Rei Abe-Yoshizumi, Susumu Yoshizawa, Hiroyasu Ito, Kazuhiro
Kogure, Hideki Kandori*. (2013) Nature Communications, 4, Article number:1678
doi: 10.1038/ncomms2689

81. Absorption Spectra and Photochemical Reactions in a Unique Photoactive Protein,
Middle Rhodopsin MR
Keiichi Inoue†, Louisa Reissig†, Makoto Sakai, Shiori Kobayashi, Michio Homma, Masaaki
Fujii, Hideki Kandori, Yuki Sudo*. (2012) Journal of Physical Chemistry B, 116, issue 20, pp
5888-5899
doi: 10.1021/jp302357m

82. L105K Mutant of Proteorhodopsin
Tushar Kanti Maiti, Keisuke Yamada, Keiichi Inoue, Hideki Kandori*. (2012) Biochemistry, 51,
issue 15, pp 3198-3204
doi: 10.1021/bi201916a

83. Transient Dissociation of the Transducer Protein from Anabaena Sensory Rhodopsin
Concomitant with Formation of the M State Produced upon Photoactivation

Masato Kondoh, Keiichi Inoue, Jun Sasaki, John L. Spudich, Masahide Terazima*. (2011)
Journal of American Chemical Society, 133, issue 34, pp 13406-13412
doi: 10.1021/ja202329u

84. Spectrally Silent Intermediates during the Photochemical Reactions of Salinibacter
Sensory Rhodopsin I
Keiichi Inoue*, Yuki Sudo, Michio Homma, Hideki Kandori. (2011) Journal of Physical
Chemistry B, 115, issue 15, pp 4500-4508

85. Chimeric Microbial Rhodopsins Containing the Third Cytoplasmic Loop of Bovine
Rhodopsin
Aya Nakatsuma, Takahiro Yamashita, Kengo Sasaki, Akira Kawanabe, Keiichi Inoue, Yuji
Furutani, Yoshinori Shichida, Hideki Kandori*. (2011) Biophysical Journal, 100, issue 8,
pp1874-1882

86. Dual Emission Caused by Ring Inversion Isomerization of a 4-Methyl-2-pyridylpyrimidine Copper(I) Complex
Michihiro Nishikawa, Kuniharu Nomoto, Shoko Kume*, Keiichi Inoue, Makoto Sakai, Masaaki
Fujii, Hiroshi Nishihara*. (2010) Journal of American Chemical Society, 132, issue 28, pp 95799581

87. Infrared Imaging of an A549 Cultured Cell by a Vibrational Sum-frequency
Generation Detected Infrared Super-resolution Microscope
Satoshi Kogure, Keiichi Inoue, Tsutomu Ohmori, Miya Ishihara, Makoto Kikuchi, Masaaki
Fujii*, Makoto Sakai*. (2010) Optics Express, 18, issue 13, pp 13402-13406

88. Development of a Non-scanning VSFG Detected IR Super-resolution Microscopeand Its Application to Biological Cells
Keiichi Inoue, Masaaki Fujii*, Makoto Sakai*. (2010) Applied Spectroscopy, 64, Number 3, pp
275-28189. Spectroscopic Studies of a Sensory Rhodopsin I Homologue from the Archaeon
Haloarcula vallismortis
Jin Yagasaki, Daisuke Suzuki, Kunio Ihara, Keiichi Inoue, Takashi Kikukawa, Makoto Sakai,
Masaaki Fujii, Michio Homma, Hideki Kandori*, Yuki Sudo*. (2010) Biochemistry, 49, issue 6,
pp 1183-1190

90. Visible-super-resolution Infrared Microscopy
Fluorescence Detected Infrared Spectroscopy

Using

Saturated

Transient

Nándor Bokor*, Keiichi Inoue, Satoshi Kogure, Masaaki Fujii, Makoto Sakai. (2010) Optics
Communications, 283, issue 3, pp 509-514

91. Characterization of a Signaling Complex Composed of Sensory Rhodopsin I and Its
Cognate Transducer Protein from the Eubacterium Salinibacter ruber

Yuki Sudo*, Akiko Okada, Daisuke Suzuki, Keiichi Inoue, Hiroki Irieda, Makoto Sakai,
Masaaki Fujii, Yuji Furutani, Hideki Kandori, Michio Homma. (2009) Biochemistry, 48, issue
42, pp 10136–10145

92. Two-point-separation in a Sub-micron Nonscanning IR Super-resolution Microscope
Based on Transient Fluorescence Detected IR Spectroscopy
Keiichi Inoue, Nándor Bokor, Satoshi Kogure, Masaaki Fujii*, Makoto Sakai*. (2009) Optics
Express, 17, issue 14, pp 12013-12018

93. Effects of Chloride Ion Binding on the Photochemical Properties of Salinibacter
Sensory Rhodopsin I
Daisuke Suzuki, Yuji Furutani, Keiichi Inoue, Takashi Kikukawa, Makoto Sakai, Masaaki Fujii,
Hideki Kandori, Michio Homma, Yuki Sudo*. (2009) Journal of Molecular Biology, 392, issue
1, pp 48-62

94. Reaction Dynamics of Halorhodopsin Studied by Time-Resolved Diffusion
Keiichi Inoue, Megumi Kubo, Makoto Demura, Naoki Kamo, Masahide Terazima*. (2009)
Biophysical Journal, 96, issue 9, pp 3724-3734

95. Functional Imaging of a Single Cell: Far-field Infrared Super-resolution Microscopy
Using Auto-fluorescence Detection
Tsutomu Ohmori, Keiichi Inoue, Makoto Sakai, Masaaki Fujii, Miya Ishihara, Makoto Kikuchi.
(2009) Proceedings of SPIE, 7182, 71820G-1-6

96. Synthesis of Pd Complexes Directly Linked to the Light-Absorbing [(bpy)3Ru]2+
Unit and Their Photochemical Reactions toward Styrenes
Akiko Inagaki*, Hiroki Nakagawa, Munetaka Akita*, Keiichi Inoue, Makoto Sakai, andMasaaki Fujii. (2008) Dalton Transactions, issue 47, pp 6709-6723

97. Signal Transmission through the HtrII Transducer Alters the Interaction of Two Helices in the HAMP Domain
Keiichi Inoue, Jun Sasaki, John L. Spudich, Masahide Terazima* (2008) Journal of Molecular
Biology, 376, issue 4, pp 963-970

98. Energetics and Role of the Hydrophobic Interaction during Photoreaction of the
BLUF Domain of AppA
Partha Hazra, Keiichi Inoue, Wouter Laan, Klaas J. Hellingwerf and Masahide Terazima* (2008)
Journal of Physical Chemistry B, 112, issue 5, pp 1494-1501

99. Photoreverse Reaction Dynamics of Octopus Rhodopsin
Keiichi Inoue, Motoyuki Tsuda and Masahide Terazima* (2007) Biophysical Journal, 92, issue
10, pp 3643-3651

100. Laser-Induced Transient Grating Analysis of Dynamics of Interaction between
Sensory Rhodopsin II D75N and the HtrII Transducer

Keiichi Inoue, Jun Sasaki, John L. Spudich, Masahide Terazima* (2007) Biophysical Journal,
92, issue 6, pp 2028-2040

101. Tetramer Formation Kinetics in the Signaling State of AppA Monitored by TimeResolved Diffusion
Partha Hazra, Keiichi Inoue, Wouter Laan, Klaas J. Hellingwerf, Masahide Terazima* (2006)
Biophysical Journal, 91, issue 2, pp 654-661

102. Diffusion Coefficient and the Secondary Structure of Poly-L-glutamic Acid in
Aqueous Solution
Keiichi Inoue, Naoki Baden, Masahide Terazima* (2005) Journal of Physical Chemistry B, 109,
issue 47, pp 22623-22628

103. Time-Resolved Enthalpy Changes of Sensory Rhodopsin II and the Transducer
Complex during Photo-Reaction
Keiichi Inoue, Jun Sasaki, Masayo Morisaki, Fumio Tokunaga, Masahide Terazima* (2005)
Journal de Physique IV France, 125, pp 769-772

104. Time-Resolved Detection of Sensory Rhodopsin II-Transducer Interaction
Keiichi Inoue, Jun Sasaki, Masayo Morisaki, Fumio Tokunaga, Masahide Terazima* (2004)
Biophysical Journal, 87, issue 4, pp 2587-2597

Review papers (all peer-reviewed)
1.

Rhodopsins at a Glance
Takashi Nagata*, Keiichi Inoue* (2021) Journal of Cell Science, 134, 22, jcs258989
doi: 10.1242/jcs.258989

2.

Microbial Rhodopsins: The Last Two Decades
Andrey Rozenberg†, Keiichi Inoue†, Hideki Kandori, Oded Béjà (2021) Annual Review ofMicrobiology, 75, pp 427-447
doi: 10.1146/annurev-micro-031721-020452

3.

Light-driven Sodium-pumping Rhodopsin: A New Concept of Active Transport
Hideki Kandori*, Keiichi Inoue, Satoshi P. Tsunoda (2018) Chemical Reviews, 118, issue 21, pp10646-10658
doi: 10.1021/acs.chemrev.7b00548

4.

Conversion of Microbial Rhodopsins: Insights into Functionally Essential Elements
and Rational Protein Engineering
Akimasa Kaneko, Keiichi Inoue, Keiichi Kojima, Hideki Kandori, Yuki Sudo* (2017)
Biophysical Reviews, 9, issue 6, pp 861-876
doi: 10.1007/s12551-017-0335-x

5.

The Light-driven Sodium Ion Pump: A New Player in Rhodopsin ResearchHideaki E. Kato*, Keiichi Inoue, Hideki Kandori, Osamu Nureki* (2016) Bioessays, 38, issue
12, pp 1274-1282
doi: 10.1016/j.tim.2014.10.009

6.

The Study and Application of Photoreceptive Membrane Protein, Rhodopsin
Keiichi Inoue (2016) Bulletin of the Chemical Society of Japan, 89, No. 12, pp 1416-1424(Invited review)
doi: 10.1246/bcsj.20160235

7.

The Study on a Novel Light-driven Sodium Pump and Creation of New Functional
Molecules
Keiichi Inoue (2016) Molecular Science, 10, Number 1, A0086 (Invited review)
doi: 10.3175/molsci.10.A0086

8.

Light-driven Ion-translocating Rhodopsins in Marine Bacteria
Keiichi Inoue, Yoshitaka Kato, Hideki Kandori* (2015) Trends in Microbiology, 23, issue 2, pp
91-98 (Recommended by “Faculty of 1000”)
doi: 10.1016/j.tim.2014.10.009.

9.

Molecular and Evolutionary Aspects of Microbial Sensory Rhodopsins
Keiichi Inoue, Takashi Tsukamoto, Yuki Sudo* (2014) Biochimica et Biophysica Acta (BBA) Bioenergetics, 1837, issue 5, pp 2587-2597
doi: 10.1016/j.bbabio.2013.05.005

10. IR Super-resolution Microspectroscopy and Its Application to Single Cells
Makoto Sakai*, Keiichi Inoue, Masaaki Fujii* (2013) Current Pharmaceutical Biotechnology,
14, issue 2, pp 159-166

Protocol paper (all peer-reviewed)
1.

Ion Transport Activity Assay of Microbial Rhodopsin Expressed in Escherichia coli
Cells
Masae Konno, Keiichi Inoue*, Hideki Kandori* (2021) Bio-protocol, 11, issue 15, Article
number: e4115
doi: 10.21769/BioProtoc.4115

Commentary articles (all peer-reviewed)
1.

Shining light on rhodopsin selectivity: How do proteins decide whether to transport
H+ or Cl–?
Keiichi Inoue* (2020) The Journal of Biological Chemistry, 295, issue 44, pp 14805-14806
doi: 10.1074/jbc.H120.016032

2.

A Series of Commentaries for A Symposium Entitled “Session 3SDA - Optogenetics:

Applying Photoreceptor for Understanding Biological Phenomena”
Satoshi P. Tsunoda*, Keiichi Inoue (2020) Biophysical Reviews, 12, issue 2, pp 295-296
doi: 10.1007/s12551-020-00674-9

Book chapters (all peer-reviewed)
1.

Diversity, mechanism and optogenetic application of light-driven ion pumprhodopsins
Keiichi Inoue
Optogenetics, Hiromu Yawo, Hideki Kandori, Amane Koizumi, Ryoichiro Kageyama eds.,(2021), 2nd. Ed., Springer Nature, Switzerland AG, Chap. 6, pp 89-126
doi: 10.1007/978-981-15-8763-4_6
ISBN 978-981-15-8762-7

2.

Super-resolution Infrared Microspectroscopy for Single Cells
Makoto Sakai, Keiichi Inoue, Masaaki Fujii
Molecular Nano Dynamics, Hiroshi Fukumura, Masahiro Irie, Yasuhiro Iwasawa, Hiroshi
Masuhara, Kohei Uosaki eds., (2009), Wiley-VCH, Weinheim, Germany, Vol. 1, Chap. 12, pp
571-588

Keynote lecture
1. Microbial rhodopsins of marine bacteria: Nano-scale biological light-driven ion pumps
Keiichi Inoue
25th 2014 International Symposium on Micro-NanoMechatronics and Human Science,
November 12, 2014, Nagoya, Japan

Invited talks
1. Photochemistry of Rhodopsins in The Microorganism World
Keiichi Inoue
ICPAC KK 2022 November 22-27, 2022, Online

2.

What Determines The Rate of Reaction Process of Ion-Transporting Rhodopsins?
Keiichi Inoue
19th International Conference on Retinal Proteins October 30-November 4, 2022, Sapporo,
Japan

3.

Discovery and Functional Analysis of Novel Retinal Proteins
Keiichi Inoue
Sir Martin Wood Prize Lecture October 17-21, 2022, Stuttgart Germany & Oxford, Cambridge,

Manchester, UK

4.

Natural Photoreceptive-Protein Toolbox of Microbial Rhodopsins
Keiichi Inoue
15th Pacific Rim Conference on Lasers and Electrooptics July 31-August 5, 2022, Sapporo,
Japan

5.

Detection of Functional Processes of Photoreceptive Proteins by Muon
Keiichi Inoue, Tamiko Kiyotani, Ichiro Tanaka
ISIS Muon Illumination Workshop, December 16, 2021, online

6.

The Diversity and The Molecular Mechanism of Light-Driven Inward Proton Pump
Rhodopsins
Keiichi Inoue
The International Chemical Congress of Pacific Basin Societies 2021 (PACIFICHEM 2021),
December 16-21, Online

7.

Photobiology of New Microbial Rhodopsins
Keiichi Inoue
BPS - Biophysical Society 65th Annual Meeting February 22-26, 2021, Online

8.

Sir Martin Wood Prize Lecture: The Emerging World of Microbial Rhodopsins in
Photobiology
Keiichi Inoue
MRC LMB Seminar November 26, 2020, Online

9.

Sir Martin Wood Prize Lecture: The Emerging World of Microbial Rhodopsins in
Photobiology
Keiichi Inoue
MPI-FKF Seminar November 11, 2020, Online

10. Spectroscopic and Structural Studies on New Types of Rhodopsins: Heliorhodopsin
and Schizorhodopsin
Keiichi Inoue
Indo-Japan workshop Frontiers in Molecular Spectroscopy_From Fundamentals to Applicationsin Chemistry and Biology October 30- November 2, 2019, Kobe, Japan

11. Spectroscopic Study on The Functional Mechanism of New Microbial Rhodopsin
Families
Keiichi Inoue
India-Japan Mini-workshop Frontiers in Molecular Spectroscopy: From Fundamentals to
Applications in Chemistry and Biology October 30- November 2, 2018, Kobe, Japan

12. Biophysical Study on Heliorhodopsin
Keiichi Inoue18th International Conference on Retinal Proteins September 24-29, 2018, Ontario, Canada

13. How the light-driven ion pump rhodopsins decide what ion species they transport?
Keiichi Inoue
The 79th Okazaki Conference: Synthetic, Biological, and Hybrid Molecular Engines August 31September 2, 2018, Okazaki, Japan

14. The New Era of Marine Bacterial Photobiology–New Types of Microbial Rhodopsin
Keiichi Inoue
3rd Global Congress & Expo on Materials Science & Engineering (GCEMSE-2018) June 14-16,
2018, Rome, Italy

15. Microbial Rhodopsins: New Types of Machinery from Marine Bacteria
Keiichi Inoue
VI International Symposium Topical Problems of Biophotonics – 2017 July 28-August 3, 2017,
St. Petersburg – Nizhny Novgorod, Russia

16. Microbial Rhodopsins: New Types of Machinery from Marine Bacteria
Keiichi Inoue
IMS Asian International Symposium “Japan–China Joint Interdisciplinary Symposium on
Coordination-based Hybrid Materials” June 23-25, 2017, Okazaki, Japan

17. Physicochemical Study on The Transport Mechanism of Novel Types of Microbial
Light-Driven Ion Pump Rhodopsins
Keiichi Inoue
International Symposium on Biophysics of Rhodopsins (Celebrating magnificent activities ofProf. Yoshinori Shichida), May 11, 2017, Kyoto, Japan

18. Physicochemical Study on The Ion-Transport Mechanism of Light-Driven Ion Pump
Rhodopsins
Keiichi Inoue
ITbM/IGER Chemistry Workshop 2016 ~student-centered symposium of forefront chemistry~,
December 19, 2016, Nagoya, Japan

19. Exploration of New Optogenetic Tools: Natural and Artificial Microbial Rhodopsins
Keiichi Inoue
The 54th Annual Meeting of the Biophysical Society of Japan, November 26, 2016, Tsukuba,Japan

20. Spectroscopic Study on The Ion-Transport Mechanism of Eubacterial Light-Driven
Ion Pumps
Keiichi Inoue
Satellite Symposium of the Retinal Protein Conference 2016 on the Molecular Reaction
Mechanism of Retinal Proteins, October 2, 2016, Potsdam, Germany

21. Light-Driven Sodium Pump Rhodopsins For Optogenetics
Keiichi Inoue
Symposium “New probes and new light: evolution of optogenetics for neuroscientific revolution”
The 39th Annual Meeting of the Japan Neuroscience Society, July 21, 2016, Yokohama, Japan

22. Light-Driven Sodium Pump Rhodopsin and Its Transport Mechanism
Keiichi Inoue
OWLS (Optics Within Life Sciences) 2016, March 18, 2016, Munbai, India

23. Spectroscopic Study on The Photo-Reaction Dynamics of Sodium Pump Rhodopsin
Keiichi Inoue
The seminar of the SFB 1078, Freie Universität Berlin, October 13, 2015, Berlin, Germany

24. Photochemistry of Sodium Pump Rhodopsin
Keiichi Inoue
DFG-Rundgespräch Photoreceptors, October 12, 2015, Frauenchiemsee, Germany

25. Infrared Spectroscopic Study on The Structure and Dynamics of Sodium Pump
Rhodopsin
Keiichi Inoue
SCIX 2015, The Great Scientific Exchange Meeting, September 28, 2015, Providence, USA

26. The Role of Proton on The Function of Sodium Pump Rhodopsin
Keiichi Inoue
The 53rd Annual Meeting of the Biophysical Society of Japan, September 14, 2015, Kanazawa,Japan

27. Function and Mechanism of Sodium Pump Rhodopsin
Keiichi Inoue
16th International Conference on Retinal Proteins, October, 7, 2014, Nagahama, Japan

28. Transient Grating Study of Microbial Rhodopsins and A New TG Technique
Keiichi Inoue
The 49th Annual Meeting of the Biophysical Society of Japan, September 17, 2011, Himeji,Japan

29. Dynamic Conformational Change of Sensory Rhodopsin II and Transducer Studied
by Transient Grating Method
Keiichi Inoue
International Conference on Recent Frontiers in Applied Spectroscopy (ICORFAS2010) September, 23, (2010) Tamil Nadu, India
30. Transient Grating Studies on Photo-Reaction Dynamics of Archeal Rhodopsins
Keiichi Inoue
Prof. Mathies Laboratory in University of California Berkeley August, 6, (2010) Berkeley, USA

Brief publication statistics
3,888 citations, h-index: 37 (Google Scholar).

Awards1.

2019 Sir Martin Wood Prize by Millennium Science Forum (November 23, 2019)2.

26th IUPAC International Symposium on Photochemistry, Awards for excellent
presentations:Youngresearcheroral

presentation

awards,Photochemical&

Photobiological Sciences
Title: “Spectroscopic and Structural Studies on Light-driven Sodium Pump Rhodopsin”(April 8, 2016)3.

Research Funds to Promising Young Molecular Scientists by The Morino Foundation for
Molecular Science (August 31, 2014)

4.

The Young Scientists’ Prize, The Commendation for Science and Technology by the
Minister of Education, Culture, Sports, Science and Technology, Japan (April 26, 2014)

5.

Investigator's Award of Research Foundation by Research Foundation for Opto-Science andTechnology (February 13, 2014)

Ad hoc reviewer to: Nature Structural & Molecular Biology

Bioenergetics

 Nature Microbiology

 Biochimica et Biophysica Acta (BBA) - Nature Communications

Biomembranes

 PNAS Nexus FEBS Letters

 Communications Biology

 BioEssays

 iScience Frontiers in Chemistry

 Scientific Reports

 Biophysics and Physicobiology

 Angewandte Chemie International Edition

 International Journal of Molecular Sciences Journal of Physical Chemistry Letters mSystems

 The Journal of Physical Chemistry B Journal of the Physical Society of Japan Biochemistry Life ACS Physical Chemistry Au

 Molecules Journal of Photochemistry and Photobiology

 Chemical Science

Applied Sciences Chemistry Biochimica et Biophysica Acta (BBA) - Methods and Protocols

Organizing committee member
1. 19th International Conference on Retinal Proteins -ICRP2018- Local Executive Committee
Member (2018-2022)
2. 16th International Conference on Retinal Proteins -ICRP2014- Local Executive Committee

Member (2014)