A case of gastric cancer in breeding Japanese Black Cattle

295/06:143.&#$*+-',29º~®3)0oúWP@4) )9¸85)

ÜÅ>]f5) `D²5)3)ú>×}}(1588.2238 Ã§°ï,Èí~946.3)
4)A¦)Fc}jvk%(1335.:879 A¦?f¦¨võ3.3.3)5)o)×}1Æa(1256.:84: öî°Ö>ÈA45X657.3)0}-BÐ$º~ ®
TEL$26:.793.546:
FAX$26:.794.4953
e-mail$kongayayamada@yahoo.co.jp!87"
öî°SpåNËF_M=J¼uÓWç¥!Ê!32ÁAê{^ìOSÒþN|GJ"|ÌSH5<ÞÚ±TbyntO$baSRñN!ÉMrTa;GJ"WJejT¼éñxój!KTNéN<KJ"­õ¯¿NT!AST ó0!γ.GT ó0!ÿmr

smv0SäßA±\_J"sà­S­¤onpt%hqvS÷NT!T ?[U B ÂlS:0A²
4N<KJ"[~mÚ±NT*Ä|!"?[UQRÔ[iAÕ2GM?]![~ýèÚ±NTÔÂlT3äYQ=GPÂlSª(Nû·#aªôGM=J"qâT¥SsS|N<^
Oð/F_J",g%u%l$|!¼u¥!(%)&"!29"ÔóÏTºRQ^VPUâH^AÞÚ±S¬$¼uÓ!32ÁSÊ¥A!

ºCQ^"G@G¥NTºRQ^OõÆóA

\WRa;G! ?[Uê{S:QP:H^JX!OyÙdF_^EOA&C!eæ(AwGJJX!o)_%Fc[RdsióSS­[QPaÛB!ÔóÏR

|GJ"
7/!ëRò324|ÌSÞÚ±T'5:#H^k»TÝQ="Q@NY|R?D^ÔóÏT¡N<]!*|RJEÔZgäY'min!µ£ò3:'min N³

SUõ*3+Z!cvfiÓS*Ç|R?D^ù!$Sbynt!ZÑQáFN$ûRH^k»*9+A<^YSS!¼u

baR²4QRñCALX\_!ÉMrR

ÓR?D^ÞSk»TVObP±BJ\Q

TA±\_J"ejT¼éñxóN!KT="½!âaÒþOGM|G!.G«

NéN<KJ"IS¶SÒzâæR4iQ

ShÀR[^^üA¢`_J¼uÓWç¥C!*34[IRTQ¹Cµ£øT!ê{Où!

R!©G!m¯R[]*Ä|A´US={óûØ@S7>L!zÍ¾+RKJ"ÞâæOOð/F_JSNISzRL=Mk»HÓ¯¿[]Ø@Sã?[U.G«ShÀ^"R[^^üOð/!w¶^ OV/GMx&3;&áÎ!ê{OJournal of Japanese Society for Clinical Infections Disease
in Farm Animals Vol.4 No.1 2009

6AD8?;3(&'*+1),:C¥Cö+ÿR59B>="

Table 1. Blood biochemical examination

 w  ¡  ~&Ht!RBC!WBC*« å Examination item

First admission

Day 12Îà+!Õ!.Öï
*`jn[]ý+!

4RBC (10 /μl)

752623S m c f p*BCP ý+! ã*Gluc,DH·UK

Ht (%)

35.8

30.6

Hb (g/dl)

11.7

10.2

Glu (mg/dl)

10099

ý+!BUN*UV ý+!Cre*i [ b V ý+!ÉWnZ\o'm*Æý+!LDH*SFBC ý+!

TP (g/dl)

96.6

CPK*GSCC ý+RÎà;="H="´ïAlb (g/dl)

1.8

1.7

eajn'YkpDÂ*bo'XT]gA/G

0.21

0.35

BUN (mg/dl)

13.639.9]l'ý+K­¨;="Cre (mg/dl)

0.86

1.13

<D~!*uªCE!SmcfpØ5MFCa (mg/dl)

8.7

7.6A)G òD ß | L É W n Z \ o ' m D ß |!

iP (mg/dl)

5.8

9.4

Mg (mg/dl)

1.74

1.66

Tcho (mg/dl)

55

39AST ¿Ø!γ,GT ¿Ø!LDH ¿ØLSph^SØ!É`lm`pØDµ³7INQ="Tbil (mg/dl)

5.32

5.55

Ô./öéCE BUN Ø5MF AST ¿Ø!CKDbil (mg/dl)

3.32

3.52

LDH (IU/l)

6137

4629

AST (IU/l)

255

560

éDw¡@E PT ª7âCÙy;γ-GT (IU/l)

1384

1339

?4=*Table/+"æéDUod'_'CM

CPK (IU/l)

158

1848

¿Ø7:NCµ³;=*Table.+"Ô/öPsë¡D~@Eâ`lm`pë@3
PA»×:Q=*Table0+"
H=*uªD WBC EâCÊ};?4=

ALP (IU/l)

1055

1817

NEFA (μEq/l)

540

610

Ammonia (mg/dl)

571.3

TBA (mg/dl)

227.9At the time of first admission!Decreased levels of albumin and A/G ratio andtotal cholesterol.
!Elevated AST and γGt and LDH activities, andincreased levels of ammonia and total bilirubin.
At the time of day 12!Still more elevated AST and CK activities, andincreased levels of BUN.

7!Ô./öéCE:NCÊ};="Ú¾E*uªCòGÊ};=7!Õ¾EñÑC²;=""´ïeajn'YkpÂD~!CD0!CD1#CD25MF MHC classq)¿£þDßØ7$,@3O!Ô./öéCE
<QND£þD²EMOÜCìJNQ=*Table1+"Table 2. Coagulation of blood examinationèáö+°&Ô¦tøD(C58P®

Day 20Ê·!Ë5MFîC58PÓ±ï¹½'PLTÃDÐð!%îD÷Ïlp_ÃDÜ;4®
0¿®Ó!%#çCÊ·;=®0!ÉÞ!

28.6

PT59

PPT

32.7

At the time of day 2!Prothrombin time(PT) was a little longer.

Á#5MF<Dû©C58Pü¢¿DÄ
À!{/!ù.!DÜ;4¼®¿ôBNFCz1ù¼DÊ-7INQ="®0Ê·E%þ7êä¶CÊ·ºÝ;!(D&ÈCK®0

î%Ô¦t%÷Ïlp_ÃrCEINQB6

Ê·7§¤;=*Fig$.+"Ô¦tD®0Ç¸@>=*Fig$.#/#0+"E®0£þExÛµóB4;í£þDÒ@Å

ö+Ç¸ á°&Ô¦t@E!vÍE¯ÈÌRÀ;?4=*Fig$.+"ËCINQ=µóCõú:Q?4=7! ÍEµó®0£®0Ç¸EÔ¦tD®0Aæ¬@!êä¶CÊ

>@-=B90<:42.7$/"5 #!!%

( /- (A case of gastric cancer in breeding Japanese Black Cattle

T0?YJ5V`/@1"91INiO7fn

Table 4. Peripheral leukocyte population

8m@!INi7GP.MgE6L>@1"lExamination item

First admission

Day 12F&('8CDBHb6:>@?RoZU4+

WBC

120

199

*'"e6-,3<caSYJ7K_21&Fig#

Mononuclear call

31.3

23.8

Granulocyte

88.7

175.2

CD3+CD45R

5.1

0.7+

CD3 CD45R

1.50.2

CD3CD45R+

8.1

5.1+

CD4

4.8

0.1

CD8+

1.7

0.1

AQ0\.L>@1"I$e$h^CDBX=
kjb6<RoW]T[.d;>@1"+

Table 3. Urinalysis
Day 2

CD4 /CD8 #

2.79

0.10

Outward appearance

dark brownish yellow

MHCclass "+CD14

4.4

2.3

Cloudy

MHCclass "+CD14+

0.6

0.4

Albumin

30mg/dl

MHCclass " CD14

16.2

14.4

Glucose

Ketone bodies



Bilirubin>+++Occult bloodUrobilinogen

0.1

pH

8.1

Specific gravity

1.031

++

+

2

Unit is 10 /μl
At the time of first admission!The slight increased in the numbers of WBC wasobserved.
!Decreased in the numbers of CD3,CD4,CD8 andMHC class " active cells.
At the time of day 12!The increase in the number of WBC was observed
compared with first admission.
!Revealed a notable decrease in CD3,CD4,CD8
and MHC class " active cells.

At the time of day 2!Outward appearance was dark brownish yellow.
!Highly bilirubinuria.

Fig.1 Omasum
Left: Primary carcinoma of the omasal groove. Proliferation of carcinoma on the omasal laminae ofthe omasal groove.
Right:Carcinoma of omasal tumor forming glandular structure.

%*)%

Journal of Japanese Society for Clinical Infections Diseasein Farm Animals Vol.4 No.1 2009

6AD8?;3(&'*+1),:C

Fig.2 Liver
Left: Obstructive large thrombus of mesenterium vein (dash line). Proliferation of carcinoma in
portal vein (insert photo)
Right: Necrosis of hepatic cell. Tumor cells in lymph vessels (!).

Fig.3 Lung
Swelled mediastinum lymph was replaced with tumor tissue.

!#$"[ÒA!©Ì$®cÈ$\a?57/Ó/GBoZ\^p{q0H!Ç

GBÂ?GJE@<v.HK!ÍÉØ?GÕA´@b@]>HB?¬¶m@ÃIÍÉS1Ö4¥®cÈÉ?o Ml4|_1hLK7"404ÓqFoZ`]1572<!b_?GJNQOPA2KC;Åx3K:,Ji@¬ae@ÕR@°[?GJo×µS@°[1Yj4*'?Ô4:,7E@@!¾Ñ£^²&)#

7E@<z3K7"?A¾Ä1¡t§MÎ4!r»1\a;+8¾Ñ£^²­GIª~WuÁ?qHK772<1hLK¬ae<AU>J2<1ËH0¹ZM6½}ÆMr»<6J¡e<;+87"¾¨<4:A!ª~WGIb¦?³­3K7"X¼?i;Aª~WAªXW$ª·V47Ø?GJ´@bfº]0Hb¿¢

W<<E?Ê¡Á;+I!¡t§A¸DHK:?dI!¯-9!T y«!Ð¿!>,>,"404Ï?/,:Aª~$WsÀ=@1g287<v.HK7"¤;+Jk@ª~W±Ánw?¡t§1!C7Ï@ª>@-=B90<:42.7$/"5 #!!%% (( %A case of gastric cancer in breeding Japanese Black Cattle

Î%mßP­N9C{ÅÝPÒãq¶ÙR¿/0ÛÏO<@Zk¨ÀPÆÉ>çîMN

«=X·O=@KµP¼>vÑB[K9Z

IK9HAM=X!â¦Pdceh'^fiP

3+"â¦Pñ¶«PÖO¿m>?
*2#¡Qä}Pñ±wPÌÄL8ZsÓ±>~\[HAM=X!ËD×ò L8ZLåÖ

8IH"

P¿mÝOµº>¼C!¿mPµá>ñrCHsÓ±U8Z"A[SLà!#&%$"

P8Z¿m]Ö¸MCHµzP¦ïLQ!

*/+ê´£¬$/764$tÁ®í$05/&01-03

ñÛÞQ¿mOÂCK<Y!â¦MQ¡*0+Hong, W. S., Min, Y., Son, Y. S. and>kNZ"FPHTU:nJPyÈMCK!

Hong, S. l. 1995. J. Kor. Med. Sci. 10 :

ñÛÞ>¿%mÜOÂCK¼C164168

K9HAMM!ÇlÛ¸>x%Ô%Ü»gib*1+Johnson, L. K., Nunez, A., Bracegirdle, J.

³L!éæÍOUñ±¹ª¸>ÑTX[HA

R., Dwyer, J. R. and Konold, T. 2008. J.

M=X!¿m]Ö¸MCKµz>Ö²C!

Comp. Pathol. 138 :165168

F[>¿mOÇlCK8H=UÖ¸PW:*2+Mutoh, K. and Wakui, H. 1987. J. Vet.

O;HsÓ±U8Z"

Med. Sci. 50 : 159167

ÚÐ}O8ZNÐPä¥Õdce*3+Mutoh, K. and Wakui, H. 1988. Okajimash'^fiO<9KQ T áV B áQF[Folia. Anat. Jpn. 64 : 351356G[/3..)μ!j§¼EZ*4+HT!â¦L*4+Ohtsuka, H., Koiwa, M., Fukuda, S.,

QçX=Ngib¢P©¾O8IHAM>

Satoh, Y., Hayashi, T., Hoshi, F., Yoshino,

B[H"SHâ¦ïPëoO<@Z CD

T. and Kawamura, S. 2004. J. Vet. Med.

2,)CD6,ÙQÀ]CH>!°PmzP¦

Sci. 66 : 905909

ïLQ`a'_P¯OWIK CD2 )CD6 Ù*5+Ritchey, J. W., Marshall, C., David, C. and>§¤EZ*0+AM=X!â¦ïQëoOBrown, T. T. Jr.1996. Vet. Pathol. 33 :<9K|OñÛÞ>¯CK9HUPM

454456,,B[H"¿/0ÛÏOQBXO T áV B á

*6+ðèì#ÊØ#è½ên$/77.$tÁ
®í$10/&1/-12>ÃC?¢CH"p u=XëoO(01(Journal of Japanese Society for Clinical Infections Disease
in Farm Animals Vol.4 No.1 2009

6AD8?;3(&'*+1),:C

A Case of gastric cancer in breeding Japanese Black Cattle

Ken TAKAI1), Madoka KITAGAWA2), Hiromichi OHTSUKA3),
Toshifumi OYAMADA3), Keniciro MUTO3)
1) Kawata Veterinarian Clinic(724$1 Arai, Fukaya$shi, Saitama 366$0016)
2)The University of Tokyo Veterinary Medical Center(1$1$1 Yayoi,Bunkyo$ku,Tokyo 113$8657)
3)Kitasato University School of Veterinary Medicine(35$1,Higashi 23$bancho, Towada$shi, Aomori 034$8628)

ABSTRACT
A 10$years old femail breeding Japanese Black Cattle bred in a livestock ranch in
Aomori Prefecture was brought to our hospital with a major complaints of inappetenceand diarrhea. At the time of arrival, the characteristic clinical findings were abdominal
distention and splashing sounds in the right flank. The visible mucous membranes
appeared icteric. In addition, the stools were watery and black while the urine was darkbrownish yellow. Serum chemistry analysis revealed elevated AST and γGT activities, andincreased levels of total bilirubin. An analysis of peripheral blood leukocyte populations
revealed a notable decrease in T and B cells. Histopathological examination showed thatthe tumor cells formed glandular structures composed of cylindrical epithelial cells orcalciform cells. In this case, the diagnosis was end stage bovine gastric cancer.
"Key Word : gastric cancer, Japanese Black Cattle#>@-=B90<:42.7$/"5 #!!%! %& !