1PR
ELI
M
I
NA
RYR
EPO
RTO
NT
H
EEARL
YOOGE
NE
SISINEUM
A
NTISPA
(EU夜O
PT
E
RA
:M
A
NT
I
SPI
DAE)
H
A
R
M
A
N
D!持Morio M
A
TS
U
Z
A
K
Iand Masakiyo S
A
TOH

Biological Laboratory，FukushimaUniveど sity，Fukushima，960‑12 Japan工n 七he present study we describe the uli
ニr
as七ructures of 七heoocyte and its accompanying nurse ce11s and the fo工1icu1ar ce1ls
during the early oogenesis inAuman七ispaharmand;i (Neurop七era:Mant
ニispidae).

. harmand! is composedof abou七 160 po1y
Each ovary in Atrophic ovario工es. Just af七er emergence，each ovaど io1e is rough明

1y 1，500 um long from 七he 七ipof 七he germarium to t
こheultima七e
ovarian fol1ic工e or egg chamber. The vitellarium is composed ofa chain of 13‑15 previ七ellogenic fo1licles or egg chambers a七
successive growing stages，and each of these consis七s of a clusω
ter of 11‑13 an七erior nurse cells and a posterior oocy七e，en
由2closed by a single layer of follicular cells.

Thus，according toこained in the present study，the number of inteど con‑the resul七 ob1

nected sister cel工s in one follicle of E. harmandi does notfollow the 2n rule. The same observation holds with Chrγsopa
旦主主よ豆 (Rousse七1978a，b)，Hagenomyia 旦主主主旦豆~scalaphu~ !:ゑ思h旦
互iand 1?
ro七idricerus japonicus (Ma七suzaki，unpublished data).
The young ooeyte situated in the anteriormost region of thevitellarium，begins to grow gradually. The size of the oocyte
andthenucleusaresomewha七 biggerthanthatofthenursecells，
oocyte nucleus contains numerous small masses of 七he nucleo工arma七erial.
The oocyte and itsaccompanying nurse cells are connected
工 ular bridges or ど ing canals (1.7‑2.3
each 0七heど with the interce工

pm in diame七er). The cy七oplasm of the ring canal region is richin free ribosomes and microtubulesI and inc工udes several mito‑
chondria. This observai
ニion sugges
七s 七ha七七 he migration of theorganelles occurs most工y in the early previtellogenesis.
工n Eumantisp~ ，the oocytes during previtellogenesis is cha‑racterized by the following features; the oocy七e cytoplasm isrich in free ribosomes and contains many mitochondria and severa工Golgi bodies.

In addition， numerous rough‑surfaced endoplasmic

re七iculum of various sizes are randomly distributed in the cyto‑
plasm.

A七 七 he same 七ime，there are seen in the peど inuclear

cytoplasm a long strand of rough‑surfaced endoplasmic re七iculum，
suggesting their derivation from the outer nuclear envelope.

，

工n the posterior~~gion of the germarium the foエ icular

cells surrounding young egg chambeど s show poor developmen七
is，the flattened nuclei are observed sporadically.

ThatHowever，

3
their cytoplasmic organelles， such as free ribosomes，mitochon‑
dria，rough‑surfaced endoplasmic reticulum etc.，are not welldeveloped.

工n the subsequent stages，they grow gradually in size

as well as in number and become cuboidal on the oocyte and squa‑mous on the nurse chamber. During this process，the follicularcell organelles，such as the free ribosomes，mitochondria，rough‑surfaced endoplasmic reticulum，Golgi bodies，etc.，also increase
gradually.

The microvilli are observed in 七he space between thefollicular cells and oocyte surface，but the micropinocytosis has
not been observed.

References
Rousset，A. (1978a) Int. J. Insect Morphol.Embryol.
， 7:45‑57.
(1978b) 工nt. J. 工nsect Morphol. Embryol.
，7:59‑71.

Proc.Arthropod.Embryol.Soc.Jpn.，
1986.