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VMRD

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CACT138A

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Helper/inducer

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CACT80C

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Cytotoxic

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MY4

IgG2b

Monocyte

Coulter

CD21

GB25A

IgM

B cellVMRD

1VMRD=VMRD, Inc. (pullman, WA, U.S.A.)
Coulter=Becman Coulter (Tokyo, Japan)
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the two groups (!<0.05).

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indicate significant differences between the twogroups (!<0.05).

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Fig. 3. Changes in the numbers of CD4+ (1) andCD8+ T cells (2) in treated group ("), and control
group (!). Values are expressed as the mean 
S.E. Asterisks indicate significant differences
between the two groups (!<0.05).

Fig. 4. Changes in the numbers of CD21+ B cell
(1) and serum IgG concentration (2) in treatedgroup ("), and control group (!). Values are
expressed as the mean  S.E. Asterisks indicate
significant differences between the two groups(!<0.05).

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A. 1996. Induction of common mucosalÃ¡ B ­ýÌ;áÜHËxBW?I;ª8U

immunity by hormonally immunomodulated

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Understanding of Nutritional InfluencesNìHN"Ã¡ B ­ýÌI¡Î%`qjo

on Bovine Health J. Dairy Sci. 89 : 1292-

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beta-carotene status at parturition. J.

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Dairy Sci. 85 : 1427-1436.

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3$Horst, R. L., Goff, J. P. and Reinhardt, T.

'3'

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A.

1994.

CalciumandvitaminDrole of 1,25-dihydroxyvitamin D3. J. Cell.

metabolism in the dairy cow. J. Dairy

Biochem. 49 : 26-31.

')"Lemire, J. M. 1995. Immunomodulatory

Sci. 77 : 1936-1951. Review.

,"Kehrli, M. E. Jr. and Goff. J. P. 1989.

actions of 1,25-dihydroxyvitamin D3. J.

Periparturient hypocalcemia in cows:

Steroid Biochem. Mol. Biol. 53 : 599-602.

Effects on peripheral neutrophil and'*"|_~!sdTe!mSt^!aq

lymphocyte function. J. Dairy Sci. 72 :

h!yLp}e!vzuf"(&&,"No1188-119.

rjU6U24Z6018n\\-"Kehrli, M. E. Jr., Nonnecke, B. J. and

YC@DH$;EJ5FJ?YkP
O" QRg (/#*--+("

Roth, J. A. 1989. Alterations in bovine'+"Ohtsuka, H., Koiwa, M., Fukuda, S.,

neutrophil function during the periparturient
period. Am. J. Vet. Res. 50 : 207-214.

Satoh, Y., Hayashi, T., Hoshi, F., Yoshino,."Kehrli, M. E. Jr., Nonnecke, B. J. andT. and Kawamura, S. 2004. Changes inRoth, J. A. 1989. Alterations in bovine

peripheral leukocyte subsets in dairy

lymphocyte

cows with inflammatory diseases after

function

duringtheperiparturient period. Am. J. Vet. Res.

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profiles to milk production and feeding+ subpopulations increase T-helper-2

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P. 2006. Parturition and hypocalcemia'-"Van Kampen, C. and Mallard, B. A. 1997.

blunts calcium signals in immune cells ofEffects of peripartum stress and healthdairy cattle. J. Dairy Sci. 89 : 2588-2595.

on circulating bovine lymphocyte subsets.

''"Kimura, K., Goff, J. P., Kehrli, M. E. Jr.

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and Harp, J. A. 1999. Phenotype analysis'."}`!|_~!mSt^!of peripheral blood mononuclear cells in

pX!ixVe!KW!|c!

periparturient cows. J. Dairy Sci. 82 :b "(&&-"w]6018Z

315-319.

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CHANGE OF IMMUNE CONDITION DURING THE PERIPARTURIENT PERIOD

Changes of Immune Condition in Dairy Cows Needed the Treatment
at Calving during the Periparturient Period
Naoko Kanikawa, Hiromichi Ohtsuka, Rui Imase, Hiroko Hareyama and Seiichi Kawamura
School of Veterinary Medicine, Kitasato University(35-1, Higashi23bancho, Towada, Aomori, 034-8628, Japan)

ABSTRACTTo recognize the relationship between health and immune condition on dairy cows
during periparturient period, we analyzed peripheral leukocyte function in the cows with
no appetite and needed treatment at calving around calving. The cows observed
depression, anorexia and cold skin and treated by calcium (Ca) fluid administration(treated group; n=5) and no treatment healthy cows (control group; n=13) were used toanalyze. Lower blood Ca concentration in treated group lasted compared with control
group during this experiment, and significant differences were observed at week 1 before
calving and day 3 after calving. After calving day 3, the number of peripheral CD3+ andCD4+ T cell in treated group were significantly lower than those in control group, andlower cell numbers were observed after that. Number of CD21+ B cell in treated group
was lower during this observation, and significant lower was observed at day 3, week 2
and week 4 compared with control group after calving. In addition, serum IgG
concentration was significantly higher in treated group than that in control group at week
4 after calving. These result suggested that abnormal immune condition in dairy cows
needed treatment at calving was observed around calving.
"Key Word : dairy cow, immune function, no appetite, periparturient#!$!

Journal of Japanese Society for Clinical Infections Disease
in Farm Animals Vol.4 No.1 2009