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Cambridge Isotope Laboratories, Inc.
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Metabolomics
Amino Acid Mixtures
For Identification and Quantification

Amino acids play critical roles in biological functions as both building blocks of peptides/proteins and intermediates of various metabolic
pathways (e.g., citric acid cycle, urea cycle). These compounds are also reported to influence the pathogenesis and propagation of metabolic
disorders /disease. Other areas of research investigate amino acids in biomarker and drug-discovery studies.
To aid continued development and application, Cambridge Isotope Laboratories, Inc. (CIL) has formulated new mixturesof stable isotope-labeled amino acids. These include a mix of the canonical amino acids (MSK-CAA-1) and a mix of rare or unnatural
noncanonical amino acids (MSK-NCAA-1). The mixes are well characterized for use in quality control and qualification /quantification studies
using targeted or untargeted LC-MS methodologies.
MSK-CAA-1: Stable isotope-labeled canonical amino acidmix composition. Reconstitution with 1 mL solvent results inconcentrations of 2.5 mM (exception L-cystine: 1.25 mM).

MSK-NCAA-1: Stable isotope-labeled noncanonical amino acidmix composition. Reconstitution with 1 mL solvent results inconcentrations of 2.5 mM.

CompoundGlycine
L-Alanine
L-Arginine·HCl
L-Asparagine·H2O*
L-Aspartic Acid
L-Cystine
L-Glutamic Acid
L-Glutamine*
L-Histidine·HCl·H2O
L-Isoleucine
L-Leucine
L-Lysine·2HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan*
L-Tyrosine
L-Valine

Compoundβ-Alanine
L-Azidohomoalanine·HCl
L-Citrulline
L-Dihydroxyphenylalanine
L-Homoarginine·HCl
L-Ornithine·HCl
Sarcosine·HClAbbrev.
GlyAlaArg
Asn
Asp
Cys-CysGluGlnHisIsoLeu
LysMetPheProSer
Thr
Trp
TyrValLabel and Enrichment
13
C2, 99%; 15N, 99%
13
C3, 99%; 15N, 99%
13
C6, 99%; 15N4, 99%
13
C4, 99%; 15N2, 99%
13
C4, 99%; 15N, 99%
13
C6, 99%; 15N2, 99%
13
C5, 99%; 15N, 99%
13
C5, 99%; 15N2, 99%
13
C6, 97-99%; 15N3, 97-99%
13
C6, 99%; 15N, 99%
13
C6, 99%; 15N, 99%
13
C6, 99%; 15N2, 99%
13
C5, 99%; 15N, 99%
13
C9, 99%; 15N, 99%
13
C5, 99%; 15N, 99%
13
C3, 99%; 15N, 99%
13C4, 97-99%; 15N, 97-99%
13C11, 99%; 15N2, 99%
13C9, 99%; 15N, 99%
13C5, 99%; 15N, 99%*Compounds absent in MSK-A2-1.2. This amino acid mix comprises 17
compounds and is supplied as a 1.2 mL solution (in 0.1 M HCl).

Cambridge Isotope Laboratories, Inc.

Catalog No.
MSK-CAA-1
MSK-NCAA-1
MSK-A2-1.2

Abbrev.
β-Ala
hAHA
Cit
DOPAHarg
OrnSarLabel and Enrichment
13C3, 98%; 15N, 96-99%
1,2,3,4-13C4, 99%; 2,4-15N2, 98%
1,2,3,4,5-13C5, 98%1-13C, ring-13C6, 99%
13
C7, 98%; 15N4, 98%
13
C5, 98%
13C3, 99%; 15N, 98%

Description
Canonical Amino Acid Mix
Noncanonical Amino Acid Mix
Metabolomics Amino Acid Mix

Unlabeled mixtures
are also available.
Please inquire.

References
van Gastel, N.; Spinelli, J.B.; Sharda, A., et al. 2020. Induction of a timed metabolic collapse
to overcome cancer chemoresistance. Cell Metab, 32(3), 391-403.
Sullivan, M.R; Danai, L.V.; Lewis, C.A.; et al. 2019. Quantification of microenvironmental
metabolites in murine cancers reveals determinants of tumor nutrient availability. Elife, 8,e44235.
Lu, Y.; Wang, Y.; Liang, X.S.; et al. 2019. Serum amino acids in association with prevalentand incident type 2 diabetes in a Chinese population. Metabolites, 9(1), 14.
Haines, N.R.; Manoharan, N.; Olson, J.L.; et al. 2018. Metabolomics analysis of humanvitreous in diabetic retinopathy and rhegmatogenous retinal detachment. J Proteome Res,
17(7), 2421-2427.
Mehta, K.Y.; Wu, H.J.; Menon, S.S.; et al. 2017. Metabolomic biomarkers of pancreaticcancer: a meta-analysis study. Oncotarget, 8(40), 68899-68915.
 hen, W.W.; Freinkman, E.; Sabatini, D.M. 2017. Rapid immunopurification of mitochondria
Cfor metabolite profiling and absolute quantification of matrix metabolites. Nat Protoc, 12(10),2215-2231.

North America: 1.800.322.1174 cilsales@isotope.com | International: +1.978.749.8000 intlsales@isotope.com | fax: 1.978.749.2768 | isotope.com
MET_RSCH_AAMIX (1/26/21)
Supersedes all previously published literature