⚫

Lineup of ancillary reagents for phosphoramidite method⚫

Provides products with guaranteed low water content based on advanced
dehydration technology⚫

Customizable composition and scale-up⚫

Labels in different colors by reagent type to avoid mix-ups

Product Line-up⚫ Deblocking Reagents⚫ Phosphoramidites⚫ Activators⚫ Solvents⚫ Capping Reagents⚫ Purification Products⚫ Oxidation Reagents⚫ Common Chemicals⚫ Sulfurizing Reagents

Contents
Introduction, Nucleic acid synthesis

p. 1-4

Ancillary Reagentsp. 5-12• Deblocking Reagents• Activators• Capping Reagents• Oxidation Reagents• Sulfurizing Reagents• ÄKTA oligopilot Reagents

Phosphoramidites

p. 13-14

Dehydrated solvents

p. 15

Reagents for amidite synthesis

p. 16

•
•
•
•

Tritylation Reagents
Base Reagents
Silylation Reagents
Phosphorylation Reagents

Cleavage / Deprotection Reagents•••

Cleavage/Deprotection of nucleobase moieties
Deprotection of phosphate moieties
Deprotection of 2’ protecting group

Purification Products•••

p. 19-20

Solid-Phase Extraction Column
HPLC Column
Eluents

Common Chemicals•

p. 17-18

Drying Traps

p. 21

Introduction, Nucleic acid synthesis
Introduction
Our lineup of nucleic acid synthesis reagents focuses on ancillary reagents and dehydration
solvents for use in the synthesis of oligonucleotides using the phosphoramidite method. We
provide reagents of suitable quality for nucleic acid synthesis by leveraging our unique liquid
preparation, synthesis, dehydration, and analysis technologies developed over years of reagent
manufacturing.

Nucleic acid
“Nucleic acids” are a collective term for deoxyribonucleic acid (DNA) and ribonucleic acid(RNA). Its constituent molecule is an oligonucleotide, a structure of bases, sugars, andphosphates linked by phosphodiester bonds. “Nucleic acid medicine,” which has attracted
considerable attention in recent years, uses DNA and RNA molecules, which are the carriers ofgenetic information in living organisms, as drugs. There is a high expectation that this approach
will lead to next-generation drugs because they can act on biological molecules that cannot betargeted by conventional small-molecule agents or antibody drugs.1) Another characteristic is thatoligonucleotides, the main component of “nucleic acid drugs,” can be manufactured by organic
synthesis (mainly solid-phase synthesis) using an automated synthesizer.

Synthesis of oligonucleotide by solid-phase synthesis ～ Purification flow
Solid-phase
synthesis
Phosphoramidite method

Cleavage
Cleavage from solid support
under basic conditions

・Soild support
・Amidites

・Ammonia water・Ancillary reagents

・AMA solution

Deprotection

Purification

Deprotection of nucleic acid
three moieties, phosphate,nucleobase and sugar

Purification by reversed-phase andanion-exchange chromatography
・Solid-Phase Extraction column
・HPLC column
・Eluents-1-

Nucleic Acid Synthesis Method
Phosphoramidite method
The traditional method for synthesizing oligonucleotides is called the phosphoramidite method,which is a form of solid-phase synthesis that involves the addition of a phosphoramidite
monomer (monoamide of phosphite diester) to a solid-phase support. The fundamental reaction
to bind the amidite, which is a nucleic acid monomer, consists of 4 steps:
Step 1 detritylation→ Step 2 amidite coupling reaction→ Step 3 capping reaction→ Step 4oxidation or sulfurization. A cycle consisting of these 4 steps is repeated until the desired strand
length is obtained (see Figure 1). An automated synthesizer is used for this reaction.

Step 1

Step 2

Step 3

Step 4

Fig 1.

Reaction mechanism of phosphoramidite method-2-Reactions and reagents for the phosphoramidite method2)
Step 1 Detritylation
Step 1 is detritylation. 4,4’-Dimethoxytrityl group (DMTr group) is often used to protect thehydroxy group at the 5’ position. Since the trityl cation is stabilized by two methoxy groups, it
can be easily cleaved by treatment with a weak acid. Dichloroacetic acid or trichloroacetic acidis used as the acid.

Step 2

Coupling Reaction (Activation)

Step 2 is the coupling reaction. Condensation occurs between the nucleotide unit to bebound and the nucleoside on the solid support or the oligonucleotide being synthesized. In
general, 5-Benzylthio-1H-tetrazole (BTT), 5-Ethylthio-1H-tetrazole (ETT), or
4,5-Dicyanoimidazole (DCI) is used to activate phosphoramidite.

Step 3 Capping Reaction
Step 3 is the capping reaction. In the phosphoramidite method, an incomplete oligo that has
one less base than the desired oligonucleotide may be formed if the coupling reaction does
not progress completely and proceeds to the next step with an unreacted hydroxy groupremaining. Therefore, the 5'-OH group of the unreacted strand is acetylated to prevent
elongation reaction.

Step 4 Oxidation
Step 4 is oxidation. A phosphite ester bond is formed during phosphoramidite coupling. Thisis slightly unstable and may cause a side reaction during further elongation reactions. To
prevent this, it is converted into a stable phosphate ester. Iodine (I2) is the most commonlyused oxidizing reagent.

-3-

Purification process of nucleic acid
The oligonucleotide synthesized by solid-phase synthesis is retained on the solid supportand is thus cleavaged using a base. The protecting groups that were introduced into reactive
substituent groups during oligonucleotide synthesis cycles to avoid their participation incoupling are deprotected to complete the reaction. After cleavage and deprotection, thecrude material is separated and purified by reversed-phase or anion-exchange
chromatography to obtain the desired oligonucleotide.3)

DNA / RNA Synthesis

Cleavage from solid support・Deprotection ofphosphate and nucleobase moieties

DNA

RNA

Deprotection of
2’ protecting group

Purification by reversed-phase andanion-exchange chromatography

Desalination

Freeze drying

Fig 2. Separation/purification process after DNA/RNA synthesis-4-Ancillary reagents
Features ➢ Lineup of ancillary reagents for phosphoramidite method➢ Provides products guaranteed to have a low water content based on advanced
dehydration technology➢ Supports customization of concentration and composition➢ Supports scale-up and mass production

Fig 3. Reagent bottles and rabel colors.

Labels of different colors used for each reagent type to avoid mix-ups
Deblocking Reagents

Activators

Capping Reagents(Cap A)

Capping Reagents(Cap B)

Oxidation Reagents

Sulfurizing Reagents

Cap connection between reagent bottles and automated synthesizers⚫ The glass gallon container used for our 3 L packaging does not fit the bottle cap provided with ÄKTAoligopilot. Before use, transfer it to a container with the GL45 screw thread.

-5-

Deblocking Reagents

TolueneCode No.
045-34445
043-34441
047-34525
045-34521
040-34515
048-34511

Product Name

Pkg. Size

Water
Content

Storage
Condition≦200ppm

Keep at RT≦500ppm

Keep at RT≦0.1%

Keep at RT

Water
Content

Storage
Condition≦40ppmKeep at RT

Water
Content

Storage
Condition

500 mL

Deblocking Solution[Dichloroacetic Acid-Toluene (3:97)]

3L
500 mL

Deblocking Solution[Dichloroacetic Acid-Toluene (5:95)]

3L
500 mL

Deblocking Solution[Dichloroacetic Acid-Toluene (10:90)]

3L

DCM
Code No.

Product Name

Pkg. Size
1L

048-28923
042-28921

Deblocking Solution-1(3w/v% Trichloroacetic Acid, Dichloromethane Solution)

3L
3 L×4

042-28926

・For preparation at the time of use, use the following reagent
Code No.

Product Name

Pkg. Size

200-02402
202-02401

25 g
Trichloroacetic Acid

100 g

204-02405－

Keep at RT≦10ppmKeep at RT－

Keep at RT≦10ppmKeep at RT

500 g

044-31235

500 mL
Dichloromethane, Super Dehydrated

048-31233

3L

040-16653

25 mL
Dichloroacetic Acid044-16656

500 mL204-17915

500 mL
Toluene, Super Dehydrated

206-17914

3L-6-

Activators

Powder / Solid
Code No.

Product Name

Structure

CAS RNⓇ

044-34851

Pkg. Size1122-28-7

25 g

046-34855

500 g

051-09491

5g

059-09492 5-Ethylthio-1H-tetrazole

89797-68-2

053-09495

138-19222
161-29041
169-29042

Storage
Condition≦300ppm

Keep at RT≦300ppm

Keep at RT≦300ppm

Keep at RT≦300ppm

Keep at RT

Water
Content

Storage
Condition≦30ppmKeep at RT≦30ppmKeep at RT≦30ppmKeep at RT≦30ppmKeep at RT

5g

042-34852 4,5-Dicyanoimidazole

130-19221

Water
Content

25 g
500 g
5g

N-Methylbenzimidazolium
Trifluoromethanesulfonate

361447-89-4

N-(Phenyl)imidazolium
Trifluoromethanesulfonate

361447-81-6

25 g
5g
25 g

Solution
Code No.

Product Name

Structure013-19685 Activator Solution-1(0.25 mol/L 4,5-Dicyanoimidazole,011-19681 Acetonitrile Solution)

CAS RNⓇ

500 mL
1122-28-7
3L

Activator Soluiton-2(0.45 mol/L 1H-Tetrazole,
Acetonitrile Solution)

288-94-8015-20015 Activator Solution-3(0.25 mol/L 5-Benzylthio-1H-tetrazole,013-20011 Acetonitrile Solution)

21871-47-6

011-19701

Pkg. Size

3L

500 mL3L

010-19695

500 mL012-19699 Activator Solution-4(0.25 mol/L 5-Ethylthio-1H-tetrazole,018-19691 Acetonitrile Solution)

2.5 L
89797-68-2
3L
3 L×4

012-19694-7-

Capping Reagents

THF
Code No.
036-18991
032-18993
033-19001
039-19003
030-19011
034-19014
037-19021
031-19024

Product Name

Pkg. Size

Cap A Solution-1(10vol% Acetic anhydride/Tetrahydrofuran Soluiton)

Cap B Solution-1[Tetrahydrofuran/1-Methylimidazole/Pyridine (8:1:1)
Solution]
Cap A Solution-2[Tetrahydrofuran/Acetic Anhydride/Pyridine (8:1:1)
Solution]

Cap B Solution-2(10vol% 1-Methylimidazole/Tetrahydrofuran Solution)

Water
Content

Storage
Condition≦100ppm

Keep at RT≦100ppm

Keep at RT≦100ppm

Keep at RT≦100ppm

Keep at RT

Water
Content

Storage
Condition

3L
3 L×4
3L
3 L×4
3L
3 L×4
3L
3 L×4

Acetonitrile
Code No.
036-25385
034-25381
039-25635
033-25633

Product Name

Pkg. Size
500 mLCap B1 Solution[Acetic Anhydride-Acetonitrile (4:6)]≦100ppm

Keep at RT≦100ppm

Keep at RT－

Keep at RT

3L
500 mLCap B2 Solution[2,6-Lutidine-Acetonitrile (6:4)]

2.5 L

037-25371

034-25401

Keep at RT

500 mLCap B2 Solution[Pyridine-Acetonitrile (6:4)]

039-25375
031-25374－
3L

3L
Cap B Solution[Acetic Anhydride-2,6-Lutidine-Acetonitrile (2:3:5)]

-8-

3L

Oxidation Reagents

THF
Code No.
156-02451
152-02453

Product Name

Pkg. Size

Oxidizing Solution-2[0.1 mol/L I2・THF:Pyridine:Water(78:20:2)Solution]

Water
Content

Storage
Condition－

Keep at RT

Water
Content

Storage
Condition

3L
3 L×4

THF free
Code No.

Product Name

Pkg. Size

150-03515
158-03516

500 mL
Oxidizing Solution[Iodine Solution (abt. 0.05 mol/L)][Pyridine:Water(9:1)]

158-03511

2.5 L
3L

-9-

－

Keep at RT

Sulfurizing Reagents

Chemical modifications of the nucleic acid phosphate moiety include phosphorothioate
modification where O (oxygen atom) is substituted by S (sulfur atom). In nucleic acid medicine,
sulfurizing modification is used mainly for antisense drugs.4) Phosphorothioate synthesis isachieved by having a sulfurizing reagent act on the phosphite ester formed by a coupling
reaction.

Powder / Solid
Code No.

Product Name

Structure

3H-1,2-Benzodithiol-3-one
324-72121 1,1-Dioxide【Beaucage reagent】
027-19422

CAS RNⓇ

Pkg. Size

Water
Content

Storage
Condition

66304-01-6

500 mg－Keep at2-10 degrees C.

－

Keep at RT

25 g

Bis(phenylacetyl) Disulfide【PADS】

15088-78-5

021-19425

500 g

166-28251

5g

164-28252 5-Phenyl-3H-1,2,4-dithiazol-3-one

7047-10-1

168-28255

25 g≦200ppm

Keep at2-10 degrees C.

500 g

The most commonly used reagent, DDTT, is highly crystalline and hardly dissolves in acetonitrile,
the reaction solvent. Therefore, acetonitrile is spiked with pyridine to increase its solubility. On theother hand, 5-phenyl-3H-1,2,4-dithiazole-3-one shows favorable solubility in acetonitrile and is thus a
convenient sulfurizing reagent that can be used pyridine-free.

-10-Sulfurizing Reagents

Solution
Code No.

Product Name

Structure

CAS RNⓇ

199-18751 Sulfurizing Solution
191-18755

Water
Content

Storage
Condition

100 mL
7047-10-1(0.05 mol/L 5-Phenyl-3H-1,2,4-dithiazol3-one, Acetonitrile Solution)－

Keep at RT－

Keep at RT

500 mL192-18741 Sulfurizing Solution
194-18745

Pkg. Size

100 mL
7047-10-1(0.1 mol/L 5-Phenyl-3H-1,2,4-dithiazol3-one, Acetonitrile Solution)

500 mL

Our sulfurizing solutions are distributed after the stability of the compound in organic solvents is confirmed.

[Notes]1. Sulfurizing reagents manufactured by FUJIFILM Wako Pure Chemical Corporation or FUJIFILM Wako Chemical
Corporation are distributed for research-only purposes. If you are seeking products for commercial use, please referto the related patents of the compound.
2. Code No. 324-72121 is distributed as a non-standard product without specific limitations on its use. Please refer torelated patents of the compound if you intend to use it for nucleic acid synthesis.

-11-Reagents for ÄKTA oligopilot
Features➢ Composition for automated synthesizer ÄKTA oligopilot (Cytiva)➢ Free from THF which may cause deterioration of the synthesizer flow channel➢ Can be supplied from laboratory to industrial scaleFig 4.

Code No.

Product NameÄKTA oligopilot

Pkg. Size

Water
Content

Storage
Condition

Deblocking Reagents
043-34441

Deblocking Solution[Dichloroacetic Acid-Toluene (3:97)]

3L≦200ppm

Keep at RT

045-34521

Deblocking Solution[Dichloroacetic Acid-Toluene (5:95)]

3L≦500ppm

Keep at RT

048-34511

Deblocking Solution[Dichloroacetic Acid-Toluene (10:90)]

3L≦1000ppm

Keep at RT

011-19681

Activator Solution-1(0.25 mol/L 4,5-Dicyanoimidazole, Acetonitrile Solution)

3L≦30ppmKeep at RT

011-19701

Activator Soluiton-2(0.45 mol/L 1H-Tetrazole, Acetonitrile Solution)

3L≦30ppmKeep at RT

013-20011

Activator Solution-3(0.25 mol/L 5-Benzylthio-1H-tetrazole, Acetonitrile Solution)

3L≦30ppmKeep at RT

012-19699

Activator Solution-4(0.25 mol/L 5-Ethylthio-1H-tetrazole, Acetonitrile Solution)

2.5 L≦30ppmKeep at RT

Activators

Capping Reagents
034-25381

Cap B1 Solution[Acetic Anhydride-Acetonitrile (4:6)]

3L－

Keep at RT

033-25633

Cap B2 Solution[Pyridine-Acetonitrile (6:4)]

3L≦100ppm

Keep at RT

031-25374

Cap B2 Solution[2,6-Lutidine-Acetonitrile (6:4)]

2.5 L≦100ppm

Keep at RT

034-25401

Cap B Solution[Acetic Anhydride-2,6-Lutidine-Acetonitrile (2:3:5)]

3.0 L

－

Keep at RT

2.5 L

－

Keep at RT

Oxidation Reagents
158-03516

Oxidizing Solution[Iodine Solution (abt. 0.05mol/L)][Pyridine:Water(9:1)]-12-

Phosphoramidites

Provides high-quality phosphoramidite
Features➢ Supports small scale to bulk scale➢ Supplies company-specific products in containers compatible with each synthesizer, suchas ABI, Expedite, MerMade, and ÄKTA oligopilot

Modified phosphoramidite
Nucleic acid drugs have chemical modifications of the nucleobase, phosphate, or sugar
moiety for functional improvements, such as acquisition of nuclease resistance andimprovement of binding affinity to target nucleic acids. Modifications of the sugar moiety
include 2’-position modifications and bridging modifications.
2’-Fluoro (2’-F), 2’-O-methyl (2’-OMe), and 2’-O-methoxyethyl (2’-MOE) modifications are
known 2’-position modifications. Kynamro®, a nucleic acid drug marketed in 2013, is a
representative Gapmer-type antisense, which uses 2'-MOE at both ends (wing sites) of theoligo nucleic acid to increase binding affinity to the target RNA in addition to sulfurization to
improve in vivo stability.4)5)
Bridging modifications were developed under the concept of “fixing the fluctuating
conformation of the sugar moiety by bridging.” The sugar moiety can be fixed to the RNA type(N type) by chemically modifying the 2’- and 4’-positions of the sugar moiety. This confers
excellent binding affinity to the target complementary strand nucleic acid, and furthermore,steric hindrance due to bridging is expected to improve functionality including nuclease
resistance. In 1997, 2’,4’-BNA/LNA (bridged nucleic acid) was developed by Imanishi, Obika,
and others from the School of Pharmaceutical Sciences, Osaka University.4)5)

2’-F

2’-OMeFig 5.

2’-MOE

Structure of modified nucleic acids-13-

2’,4’-BNA/RNALocked Nucleic Acid
Code No.
128-06771

124-06773

125-06781

121-06783

122-06791

128-06793

125-06801

121-06803

Product Name

Structure

CAS RNⓇ

Pkg. Size

Water
Content

Storage
Condition≦0.5%

Keep at-20 degrees C.

≦0.5%

Keep at-20 degrees C.

≦0.5%

Keep at-20 degrees C.

≦0.5%

Keep at-20 degrees C.

1g

Locked Nucleic Acid-A(Bz)
Cyanoethyl Phosphoramidite(mixture of isomers)

206055-79-0
5g

1g

Locked Nucleic Acid-G(DMF)
Cyanoethyl Phosphoramidite(mixture of isomers)

709641-79-2
5g

1g

Locked Nucleic Acid-mC(Bz)
Cyanoethyl Phosphoramidite(mixture of isomers)

206055-82-55g

1g

Locked Nucleic Acid-T
Cyanoethyl Phosphoramidite(mixture of isomers)

206055-75-65g

We supply locked nucleic acids in containers (glass bottles) that meet our specifications.

-14-

Dehydrated solvents
Acetonitrile (Low moisture guaranteed)

Features

Code No.

➢ Water content ≤10ppm guaranteed, optimal for nucleic acid synthesis due tominimal water content➢ Absorbance guaranteed (260 nm, 280 nm, 400 nm)➢ Can be supplied from laboratory to industrial scale

Product Name

Pkg. Size017-27111

Water
Content

Storage
Condition≦10ppmKeep at RT

3L
Acetonitrile, Super Dehydrated

015-27117

18 L[ Notes ]1.
Acetonitrile is available in 3 L glass bottle and returnable container for larger volumes.
2.
Returnable containers made 304 stainless steel. Coupler size is 18 L (2P on gas side, 3P on liquid side), 100L (3P on gas side, 4P on
liquid side).
3.
Please use up the regents completely and return it to the agency immediately after use.

Fig 6. Image of container(left：3L glass bottle, center / right：stainless containers）

In addition to acetonitrile, we provide solvents with guaranteed low water content for organic synthesis.
Code No.

Product Name

Pkg. Size

167-18455

Water
Content

Storage
Condition≦50ppmKeep at RT≦10ppmKeep at RT

500 mL
Pyridine, Dehydrated

165-18451

3L

204-17915

500 mL
Toluene, Super Dehydrated

206-17914

3L

-15-

Reagents for amidite synthesis

Tritylation Reagents
Code No.

Product Name

Structure

CAS RNⓇ

130-19282

Pkg. Size

Storage
Condition

25 g
4-Methoxytrityl Chloride

14470-28-1

Keep at RT

132-19281

100 g

046-34872

25 g
4,4'-Dimethoxytrityl Chloride

40615-36-9

048-34871

Keep at RT
100 g

Base Reagents
Code No.

Product Name

Storage
Condition

Pkg. Size

059-05352

N-Ethyldiisopropylamine【DIPEA】

25 mL

Keep at RT

164-05312

Pyridine

25 mL

Keep at RT

Silylation Reagents
Code No.
043-34681
041-34682

Product Name

Structure

CAS RNⓇ

Pkg. Size

Storage
Condition

5g

1,3-Dichloro-1,1,3,3tetraisopropyldisiloxane

69304-37-6

Keep at RT
25 g

Phosphorylation Reagents
Code No.
037-25751
033-25753
028-19751
026-19752

035-25671

Product Name

Structure

CAS RNⓇ

Pkg. Size
1g

2-Cyanoethyl
N,N-Diisopropylchloro
phosphoramidite

89992-70-1

Bis(diisopropylamino)
chlorophosphine

56183-63-2

2-Cyanoethyl
N,N,N',N'-Tetraisopropyl
phosphordiamidite

102691-36-1

5g
5g
25 g

-16-

5g

Storage
Condition
Keep at2-10 degrees C.

Keep at2-10 degrees C.

Keep at-20 degrees C.

Cleavage / Deprotection Reagents
Cleavage/Deprotection of nucleobase moieties
The crude oligonucleotide obtained by solid-phase synthesis remains bound to the solid
support. After synthesis, it is cleavaged from the solid support by ester hydrosis with a base suchas concentrated ammonia water. At the same time, the protecting group introduced into theamino group of the nucleobase moiety is removed under basic conditions. Instead ofconcentrated ammonia water, methylamine solution, AMA solution (a mixture of concentrated
ammonia water and methylamine solution), or potassium carbonate/methanol solution may be
used for this reaction depending on the type of protecting group.

Code No.

Product Name

Pkg. Size

Storage
Condition

017-03176

25% Ammonia Solution

500 mL

Keep at RT

132-01856

40% Methylamine Solution

500 mL

Keep at RT-17-

Deprotection of phosphate moieties▮ Deprotection of 2-cyanoethyl group
The 2-cyanoethyl group, which protects the phosphate moiety, generates acrylonitrile as a byproduct when deprotected with concentrated ammonia water. Acrylonitrile may cause
2-cyanoethylation through addition reaction to the nucleobase moiety. As a method to avoid theside reaction of acrylonitrile, a weak base reagent such as diethylamine/acetonitrile solution,
instead of generally used concentrated ammonia water, can be used to deprotect the
2-cyanoethyl group while maintaining binding with the solid support, followed by cleavage fromthe solid support and deprotection of the protecting group of the nucleobase moiety.

Code No.

Product Name

045-34825

20% Diethylamine Acetonitrile Solution

017-27111

Acetonitrile, Super Dehydrated

Pkg. Size

Storage
Condition

500 mL

Keep at RT

3L

Keep at RT

Deprotection of 2’ protecting group
For the purpose of RNA synthesis, the hydroxy group at the 2’-position of the sugar moiety isalso protected. A tert-butyldimethylsilyl group (TBDMS group) or triisopropylsilyloxymethyl group(TOM group) is commonly used as the protecting group. Both TBDMS and TOM groups can be
deprotected by treatment with fluoride ions such as tetrabutylammonium (TBAF) solution.3)

TBDMS group

Code No.

Product Name

208-20201

Tetrabutylammonium Fluoride,
Tetrahydrofuran Solution (ca. 1 mol/L)【TBAF】

200-20205

Pkg. Size
100 mL
500 mL

352-34922

TOM group
Storage
Condition
Keep at2-10 degrees C.

25 g
Triethylamine Trihydrofluoride

Keep at RT

350-34923

100 g

-18-

Purification Products
Liquid chromatography-based nucleic acid separation is widely used for oligonucleotide
purification and analysis. Reversed-phase chromatography is based on the principle ofseparation using differences in hydrophobicity and uses a support of silica gel C18. Anionexchange chromatography is based on the principle of separation using the polyanionic nature of
nucleic acids and uses a support of an anion-exchange resin (rigid polymer, etc.) that is positively
charged to capture anions.3)

Solid-Phase Extraction column (SPE column)
Solid-phase extraction is a separation and purification technique to extract and purify the target
material using a small-sized column packed with a silica gel or polymer gel support. Duringoligonucleotide pretreatment, the synthesized crude material is passed through a simple
reversed-phase resin column (solid-phase extraction column) for contaminant removal anddetritylation. This technique is suitable for pretreatment of small-volume samples.
Our Presep® DNA/RNA is a solid-phase extraction column suitable for oligonucleotide
pretreatment. Type A uses a silica gel support and achieves high-efficiency and highperformance purification. It can be used as a sample pretreatment tool for HPLC or LC/MS
analysis.
Features⚫ Achieves high sample load
⚫ Can load a sample volume 3 to 5 times larger
than that of commercial pretreatment columns⚫ Excellent deprotection efficiency⚫ High purification capacity⚫ High recovery

Code No.
290-36691
296-36693
290-36711
296-36713

Product Name

Fig 7. Presep® DNA/RNA Type A

Pkg. Size

Storage
Condition

20 pieces

Presep® DNA/RNA Type A (85 mg/1 mL)
Synthetic scale：0.2-0.5 μmol

Keep at RT
50 pieces

Presep® DNA/RNA Type A (255 mg/3 mL)
Synthetic scale：1-1.5 μmol

20 pieces
Keep at RT
50 pieces292-36891

Presep® DNA/RNA Type A (1.0 g/15 mL)
Synthetic scale：4-6 μmol

10 pieces

Keep at RT

292-36911

Presep® DNA/RNA Type A (1.7 g/25 mL)
Synthetic scale：6-10 μmol

10 pieces

Keep at RT

299-36921

Presep® DNA/RNA Type A (5.1 g/70 mL)
Synthetic scale：20-30 μmol

10 pieces

Keep at RT

HPLC column（ODS column）
Wakopak® Ultra Series C18 is a highly durable ODS column using high-purity spherical silica gel.
Code No.

235-02651

Product Name

Pkg. Size

Wakopak® Ultra C18-5
Φ 4.6 mm × 150 mm (W)

1 piece-19-

Storage
Condition
Keep at RT

Eluents
A mixture of an organic solvent (acetonitrile, methanol, etc.) and a buffer (triethylamine-acetic
acid, etc.) is used as an eluent for reversed-phase chromatography. As the buffer for nucleic acidseparation, triethylamine-acetic acid, which is easily sublimated by freeze-drying, is generally
used.3)
Code No.

Product Name

Pkg. Size

019-08631

1L
Acetonitrile

Keep at RT

015-08633

202-13131

Storage
Condition

3L
2 mol/L Triethylamine Acetate Solution pH 7.0【 TEAA solution 】

085-06991

200 mL

Keep at RT

100 mL
1,1,1,3,3,3-Hexafluoro-2-propanol

Keep at RT

087-06995

500 mLThe products listed below are buffer products for nucleic acid synthesis manufactured anddistributed by Nippon Gene Co., Ltd. These products can be used as dissolution solvents forsalts or nucleic acids to be added to nucleic acid solutions for ethanol precipitation. Since weprovide a custom buffer manufacturing service, we can manufacture custom-made buffers
according to your request.
Code No.

316-90081

Product Name

Pkg. Size

3 M Sodium Acetate (pH 5.2)

100 mL

314-90021
310-90023

Keep at RT

100 mLTE (pH8.0)[10 mmol/L Tris-HCl (pH 8.0)1 mmol/L, EDTA (pH 8.0)]

100 mL×6

316-90025

500 mL

316-90101

100 mL

312-90103

Storage
Condition

100 mL×6

Distilled Water, Deionized318-90105

500 mL-20-

Keep at RT

Keep at RT

Common Chemicals
Drying traps
We offer zeolite packed in non-woven fabric pouches. By wrapping zeolite in non-woven fabric,crushed zeolite powder is prevented from being mixed into the solvent. After use, zeolite can beremoved from the bottle with the non-woven fabric pouch, facilitating aftertreatment. Use theseproducts for dehydration of amidite reagents or ancillary reagents.
➢ Shape: spherical➢ 3 sizes: 3 g, 10 g, and 50 g
⇒ Selectable according to container size
➢ Highly airtight aluminum outer bag (degassed inside aluminum bag)
➢ Non-woven fabric material: composite fiber ofpolypropylene/polyethylene

3g
Fig 8.

Code No.
261-02271

Product Name
Zeolite Packs[Zeolite, Synthetic, A-3, Beads, 1.40-2.36 mm (8 - 12mesh)]267-02273
265-02274

10 g

Zeolite pack each capacity

Pkg. Size

Storage
Condition

3 g×20
10 g×20

・Reference size of non-woven fabric
3 g：60 mm×65 mm, 10 g：120 mm×65 mm, 50 g：120 mm×65 mm

50 g

Keep at RT

50 g×10【Notes】
The moisture absorption capacity of synthetic zeolite is approximately 25% of its own weight. Add approximately 3 to 4 times
the calculated amount of synthetic zeolite to your solvent.

Regular bottled products are also available. Please use them if non-woven fabric is not required.
Code No.

Product Name

Pkg. Size

Storage
Condition

263-00575

Zeolite, Synthetic, A-3, Beads, 1.40 - 2.36 mm (8 - 12mesh)

500 g

Keep at RT

133-08645

Molecular Sieves 3A 1/8

500 g

Keep at RT

134-06095

Molecular Sieves 3A 1/16

500 g

Keep at RT-21-

〔References〕
1) [2016 Edition: Current status and future prospects of global nucleic acid drug development] 2016: Sekai no
kakusaniyakuhinkaihatsu no genjyo to syoraitenbou (in Japanese). Seed Planning, Inc. (2016) .
2) [Biotechnology reagents] Baiotekunoroji shiyaku (in Japanese). The Chemical Daily Co., Ltd. (1989).
3) Hirao Ichiro., Kurumizaka Hitoshi., [Principles and protocols of nucleic acid experiments that can be selected according topurpose] Mokutekibetsu de eraberu kakusanjikken no genri to purotokoru (in Japanese). Yodosha Company, Ltd. (2011).
4) Takao Inoue., : Drug Delivery System., 31-1, 10 (2016). (Japanese)5) ”Development and Applications of Nucleic Acid Therapeutics” ed. by Takeshi Wada., CMC Publishing Co.,Ltd.
(2016). (Japanese)

2 3 X0 . 0 5 学 01R-22-