May 15 (Fri)-16(Sat), 2009

The 7th Stem Cell Research Symposium
Program

Date： May 15 (Fri) - 16(Sat), 2009

Venue

： Izumi Garden Gallery

Director ：Hideyuki Okano
Department of Physiology, Keio University School of Medicine

Organizer ：Stem Cell Research Symposium
Cosponsor：Keio University Global COE Program
Education and Research Center for Stem Cell Medicine

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7th Stem Cell Research Symposium

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Friday, May 15.

The First Day

Registration・Exhibit posters
Opening Remarks

Director

9:00～9:30
Hideyuki Okano

9:30～9:40(Department of Physiology, Keio University School of Medicine)

Session 1：Hematopoietic Stem Cell
Chair Mineo Kurokawa

9:40～11:00(Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo)
O-1

Characterization of hematopoietic clusters in the mouse embryo
Tomomasa Yokomizo and Elaine Dzierzak(Erasmus University, Department of Cell Biology, Rotterdam,
The Netherlands )O-2

Characterization of hematopoietic stem cells in adult fish kidney
Isao Kobayashi1, Tadaaki Moritomo2, Toshio Suda1(1Department of Cell Differentiation , School of Medicine, Keio University,
2Department of Veterinary Medicine, College of Bioresource Sciences,
Nihon University)

O-3

Importance of Notch signals in hematopoietic homeostasis
Yukari Muguruma1, Takashi Yahata2, Yin Sheng1, Katsuto Hozumi3,
Kiyoshi Ando4(1Division of Hematopoiesis, Research Center for Regenerative Medicine,
2Department of Cell Transplantation and Regenerative Medicine,
3Department of Immunology, 4Department of Hematology,
Tokai University School of Medicine)O-4

Derivation of functional mature neutrophils from human embryonic stem cells
Yasuhisa Yokoyama1, 2, 3, Takahiro Suzuki1, 3,
Mamiko Sakata-Yanagimoto1, 2, 3, Keiki Kumano1, 3, Katsumi Higashi4,
Tsuyoshi Takato5, Mineo Kurokawa3, Seishi Ogawa1, 5, 6, andShigeru Chiba1, 2(1Department of Cell Therapy and Transplantation Medicine, University of Tokyo
Hospital, Tokyo; 2Department of Clinical and Experimental Hematology,
University of Tsukuba, Tsukuba; 3Department of Hematology and Oncology,
Graduate School of Medicine, University of Tokyo, Tokyo; 4Department of Clinical
Hematology, School of Health Sciences, Kyorin University, Tokyo; 5Divison of
Tissue Engineering, University of Tokyo Hospital, Tokyo; 6The 21st Century COE
Program, Graduate School of Medicine, University of Tokyo, Tokyo)

Special Lecture 1

11:00～11:40

Glycome analysis during differentiation of human embryonic stem cells
John Yu（The Genomics Research Center, and Institute of Cellular & Organismic
Biology Academia Sinica, Taiwan）

Poster Session

11:40～13:50

Gallery B：lunch served

Secretary society

12:20～13:00

Working Room 2

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7th Stem Cell Research Symposium

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Session 2：Mesenchymal Stem Cell and Angiogenesis
Chair Hiroshi Asahara

13:50～15:10(Department of Systems BioMedicine, National Research Institute for Child
Health and Development)
O-5

Co-transplantation of MSCs improves the engraftment of HSCs in
nonhuman primates
Shigeo Masuda1, Naohide Ageyama2, Hiroaki Shibata2, Yoko Obara3,
Tamako Ikeda1, Kengo Takeuchi4, Yasuji Ueda5, Keiya Ozawa3,
Yutaka Hanazono1(1 Division of Regenerative Medicine, Center for Molecular Medicine, Jichi
Medical University, 2 Tsukuba Primate Research Center, National Institute of
Biomedical Innovation, 3 Division of Hematology, Department of Medicine,
Jichi Medical University, 4 Division of Pathology, The Cancer Institute,
5 Department of Gene Therapy, Graduate School of Medicine, Chiba University)

O-6

Prospective identification of Murine and Human Mesenchymal Stem Cells
Yumi Matsuzaki1, Satoru Morikawa1, 2, Yo Mabuchi1 and
Hideyuki Okano1(1 Department of Physiology, Keio University School of Medicine, 2 Departmentof Dentistry and Oral Surgery, Keio University School of Medicine)

O-7

Converged signaling of Notch and -catenin in VEGFR2+ vascular progenitor
cells confers arterial-venous specification
Kohei Yamamizu1, Jun K.Yamashita1,2(1 Laboratory of Stem Cell Differentiation Stem, Cell Research Center,
Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan,
2 Center for iPS Cell Research and Application, Institute for Integrated
Cell-Material Sciences, Kyoto University, Kyoto, Japan)

O-8

Snail is required for TGF--induced endothelial-mesenchymal transition ofembryonic stem cell-derived endothelial cells
Tetsuro Watabe1, Takashi Kokudo1, Yuka Suzuki1, Yasuhiro Yoshimatsu1,
Tomoko Yamazaki1, Kohei Miyazono1(1Department of Molecular Pathology, Graduate School of Medicine, Universityof Tokyo)Special Lecture 2

15:10～15:50

The therapeutic plasticity of neural stem/precursor cells
Stefano Pluchino（CNS Repair Unit, DIBIT II and Institute of Experimental Neurology (InSpe),
San Raffaele Scientific Institute, via Olgettina 58, I-20132 Milan, Italy）

Coffee Break7th Stem Cell Research Symposium

15:50～16:20

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May 15 (Fri)-16(Sat), 2009

Session 3：Neural Stem Cell
Chair Hideyuki Okano

16:20～17:40(Department of Physiology, School of Medicine, Keio University)
O-9

Role of TEAD1 in the proliferation of retinal pigment-cell progenitors
Michinori Kitagawa1, Takumi Era2
(1Signal Regulation, 2Molecular Neurobiology,
Embryology and Genetics, Kumamoto University)

O-10

InstituteofMolecular

Tsukushi is a Frizzled ligand that regulates the proliferation of neuronal
stem/progenitor cells
Kunimasa Ohta1, Ayako Ito1,2, Yohei Shinmyo1, Hideaki Tanaka1,2(1Department of Developmental Neurobiology, Graduate School of Medical
Sciences, Kumamoto University, 2Global COE, Kumamoto University)

O-11

A vascular niche factor for neural stem cells
Jun Namiki1, Sayuri Suzuki2, Shinsuke Shibata3, Yumi Matsuzaki3,
Hideyuki Okano3(1Department of Emergency and Critical Care Medicine, 2Center of Integrated
Medical Research, 3Department of Physiology, School of Medicine, Keio
University)

O-12

Committed neuronal precursors confer astrocyte-differentiation potential onneural stem cells through Notch-signal mediated DNA demethylation
during mouse brain development
Katsunori Semi1, Masakazu Namihira1, 2, Jun Kohyama1,
Tsukasa Sanosaka1, Kinichi Nakashima1(1 Laboratory of Molecular Neuroscience, Nara Institute of Science andTechnology, 2 Department of Human Genetics, David Geffen School of Medicine,
University of California at Los Angeles)

Special Lecture 3

17:40～18:20

The Use of Stem Cells or Their Progenitors in Repair of Myelin Disorders
Ian D. Duncan（University of Wisconsin-Madison, Madison, WI, USA）

General Meeting

Chief Director Toshio Suda

18:20～18:40(Department of Cell Differentiation, Keio University School of Medicine)3

7th Stem Cell Research Symposium

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Poster Session

Friday, May 15

11:40～13:50

Gallery B
P-1

Human pluripotent stem cell-derived neurosphere culture based neuraldifferentiation control method and application
Tamaki Wada1, Norie Tooi1, Makoto Honda1, Kazuhiro Aiba1,
Norio Nakatsuji2(1 Stem Cell and Drug Discovery Institute, 2 Institute for Integrated
Cell-Material Sciences (iCeMS) and Institute for Frontier Medical Sciences,
Kyoto University)

P-2

Analysis of Hematopoietic Cells in C/EBPß and C/EBPε Double Knockout
Mice
Tadayuki Akagi1, 2, Nils H. Thoennissen2, Gay Crooks3,
Adrian F. Gombart2, H. Phillip Koeffler2(1Department of Stem Cell Biology, Institute of Medical, Pharmaceutical and
Health Sciences, Kanazawa University, 2Division of Hematology and Oncology,
Cedars-Sinai Medical Center, UCLA School of Medicine, 3Division of PediatricHematology/Oncology, Children’s Hospital Los Angeles)

P-3

Foxo3a is essential forleukemia-initiating cells

maintenanceofchronic

myelogenous

Kazuhito Naka1, Takayuki Hoshii1, Teruyuki Muraguchi1, Takako Ooshio1, 2,
Noboru Motoyama3, and Atsushi Hirao1, 2(1Center for Cancer and Stem Cell Research, Kanazawa University, 2CREST,
JST, 3Department of Geriatric Medicine, National Center for Gerontology andGeriatrics)

P-4

Multiple developmental defects in R-spondin2 knockout mice
Wakako Yamada1,3, Keiyo Takubo1, Kenji Nagao4, Kaori Horikoshi3,
Ayako Fujikura3, Eiji Ikeda2, Yoshimasa Inagaki3, Makoto Kakitani3,
Kazuma Tomizuka3, Hiroshi Miyazaki3, Toshio Suda1(1Department of Cell Differentiation, 2Department of Pathology, School of
Medicine, Keio University, 3Innovative Drug Research Laboratories, 4Frontier
Research Laboratory, Kyowa Hakko KIRIN Co., Ltd.)

P-5

Migration, localization, and differentiation of neural crest-derived entericneural precursor cells in aganglionic gut
Naoki Shimojima1, Yasuhide Morikawa 1, Ryuhei Nishikawa 1,
Shinsuke Shibata 2, Ryo Hotta 1, Narihito Nagoshi 3, Masaya Nakamura 3,
Yumi Matsuzaki 2, Hirotaka J Okano 2, Hideyuki Okano 2(Department of 1 Pediatric Surgery, 2 Physiology, and 3 Orthopaedic Surgery,
Keio University School of Medicine, Tokyo, Japan)

P-6

Marked Variation in the Safety and Therapeutic Potential of Induced
Pluripotent Stem Cells
Kyoko Miura1, 2, 3, 4, *,Osahiko Tsuji4, 5, *, Yohei Okada4,
Kazutoshi,Takahashi1, 2, 3, Keisuke Okita1, 2, 3, Makoto Nishino4,
Daisuke Ogawa4, Eiji Ikeda6, Yoshiaki Toyama5, Masaya Nakamura5,
Shinya Yamanaka1, 2, 3, 9, ** and Hideyuki Okano4, **(1Center for iPS Cell Research and Application (CiRA), Institute forIntegrated Cell-Material Sciences, Kyoto University, Kyoto 606-8507, Japan
2 Department of Stem Cell Biology, Institute for Frontier Medical Sciences,
Kyoto University, Kyoto 606-8507, Japan. 3CREST and Yamanaka iPS Cell
Special Project, Japan Science and Technology Agency, Kawaguchi 332-0012,
Japan. 4Department of Physiology, School of Medicine, Keio University, Tokyo
160-8582, Japan. 5Department of Orthopaedic Surgery, 6Department of
Pathology, School of Medicine, Keio University, Tokyo 160-8582, Japan.)

7th Stem Cell Research Symposium

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May 15 (Fri)-16(Sat), 2009P-7

A feeder-free production of neutrophils from human embryonic stem cells
and induced pluripotent stem cells
Kumiko Saeki1, Yoshiko Yogiashi1, Naoko Nakamura1, Satoko Matsuyama1,
Chikako Sato1, Maiko Gokoh1, Kazutoshi Takahashi2, Shinya Yamanaka2,
Akira Yuo1
(1Department of Hematology, International Medical Center of Japan, Tokyo, Japan,
2Center for iPS Cell Research and Application, iCeMS, Kyoto University)

P-8

Role of TNF- in the mesenchymal stromal cell (MSC) accumulation attumor sites
Ryosuke Uchibori, Takayuki Ito, Hiroyuki Mizuguchi, Masashi Urabe,Hiroaki Mizukami, Akihiro Kume, Keiya Ozawa(1Div. Genet. Ther., Ctr. Mol. Med., Jichi Med Univ., 2National Institute of
Biomedical Innovation)

P-9

Blocking of IL-21 signal attenuates graft-versus-host disease but notgraft-versus-leukemia effect in a mouse model
Akiko Meguro1, Katsutoshi Ozaki1, Ikekuni Oh1, Keiko Hatanaka1,
Keiya Ozawa1（1 Division of Hematology, Department of Medicine, Jichi Medical University）

P-10

Detection of Definitive Endothelial Progenitors in Mouse Bone Marrow
Mononuclear Cell Populations
Junjie Yang1, 2, Masaaki Ii1, Naosuke Kamei1, Kwon Sang-Mo3,
Yoshiki Sawa2, Takayuki Asahara3(1 Group of Vascular Regeneration Research, Institute of Biomedical Researchand Innovation, Kobe, 2 Division of Cardiovascular Surgery, Department ofSurgery, Osaka University Graduate School of Medicine, 3 Department ofRegenerative Medicine, Tokai University School of Medicine)

P-11

TET family oncogene Fus is essential for the maintenance of self-renewing
hematopoietic stem cells
Takeaki Sugawara1, Yohei Morita2, Hideyuki Oguro1, Atsushi Iwama1(1Department of Cellular and Molecular Medicine, Graduate School of
Medicine, Chiba University, 2Division of Stem Cell Therapy, Center for Stem
Cell and Regenerative Medicine, Institute of Medical Science, University ofTokyo)

P-12

Novel therapeutic strategy in patients who require repeated transfusion ofhuman leukocyte antigen-matched platelets derived from human iPS cells
Naoya Takayama1, Koji Eto1, Sou Nakamura1, Ryoko Ohnishi1,
Shinya Yamanaka2, Hiromitsu Nakauchi1(1Division of Stem Cell Therapy, Center for Stem Cell and Regenerative
Medicine, The Institute of Medical Science, University of Tokyo, 2Departmentof Stem Cell Biology, Center for iPS cell Research and Application iCeMS,
Kyoto University)

P-13

DNA methyltransferase Dnmt3b is essential for development ofhematopoietic stem cells in mouse fetal liver
Makoto Ibata1, Hideo Ema1, Masaki Okano2, and Hiromitsu Nakauchi1(1Division of Stem Cell therapy, Center for Stem Cell and Regenerative
Medicine, Institute of Medical Science, University of Tokyo, 2Laboratory for
Mammalian Epigenetic Studies, Center for Developmental Biology, RIKEN)

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P-14

Definitive proof for direct reprogramming of hematopoietic cells topluripotency
Motohito Okabe1, Makoto Otsu1, Ahn Dong Hyuck1, Toshihiro Kobayashi1,
Yohei Morita1, Yukiko Wakiyama2, Masafumi Onodera3, Koji Eto1,
Hideo Ema1, & Hiromitsu Nakauchi1, 2(1Division of Stem Cell Therapy, Center for Stem Cell and Regenerative
Medicine, Institute of Medical Science, University of Tokyo, 2Japan Science
Technology Agency, ERATO, Nakauchi Stem Cell and Organ Regeneration
Project, and 3Department of Genetics, National Research Institute for Child
Health and Development)

P-15

Molecular role of Scmh1, a member of Polycomb complex 1, which plays acrucial role for governing the hematopoietic stem cell activity
Shin’ichiro Yasunaga , Yoshinori Ohno, Motoaki Ohtsubo,
Miyuki Tsumura, Yuka Kageyama, Rie Tokimoto, Yoshihiro Takihara(Dept. Stem Cell Biol., RIRBM, Hiroshima Univ.)

P-16

A site-specific gene integration system and human embryonic stem cell lines
carrying neurodegenerative disease genes
Kazuhiro Aiba1, Kenji Sakurai1, Norie Tooi1, Makoto Honda1, Tamaki Wada1,
Miho Shimoji1, Norio Nakatsuji2, 3(1Stem Cell and Drug Discovery Institute, 2Institute for Integrated
Cell-Material Sciences and 3Institute for Frontier Medical Sciences, Kyoto
University)

P-17

SIRT1 Deficiency Suppressed the Maintenance of Hematopoietic Stem Cell
Pool
Sachiko Ezoe, Itaru Matsumura, Hirokazu Tanaka, Yusuke Satoh,Yuzuru Kanakura(Department of Hematology and Oncology, Osaka University Graduate Schoolof Medicine)

P-18

Excess amount of Eed induces apoptosis of mouse ES cells
Hiroki Ura, Tadayuki Akagi, Hiroshi Koide, Takashi Yokota(Department of Stem Cell Biology, Institute of Medical, Pharmaceutical and
Health Sciences, Kanazawa University)

P-19

TSC-mTOR signaling controls the hematopoietic stem cell pool through both
intrinsic and extrinsic pathways
Takayuki Hoshii1, Teruyuki Muraguchi1, Takako Ooshio1, 2, Kazuhito Naka1,
Atsushi Hirao1, 2(1Division of Molecular Genetics, Center for Cancer and Stem Cell Research,
Cancer Research Institute, Kanazawa University, 2Core Research for
Evolutional Science and Technology (CREST))

P-20

p53- and Ink4a/Arf-independent growth arrest of neural stem/progenitor
cells induced by oncogenic Ras signal in vivo
Teruyuki Muraguchi1, 2, Takayuki Hoshii1, Takako Ooshio1, Kazuhito Naka1,
Atsushi Hirao1, 3(1Division of Molecular Genetics, Center for Cancer and Stem Cell Research,
Cancer Research Institute, Kanazawa University, 2Japan Society for the
Promotion of Science, 3Core Research for Evolutional Science and Technology(CREST), Japan Science and Technology Agency)

P-21

Kinetics of CXCR4 expression on murine steady state KSL cells
Yutaka Sasaki, Yoshikazu Matsuoka, Takayuki Toyohara, Makoto Hase,
Ryusuke Nakatsuka, Yasushi Uemura, Yoshiaki, Sonoda(Department of Stem Cell Biology and Regenerative Medicine, Graduate
School of Medical Science, Kansai Medical University)

7th Stem Cell Research Symposium

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P-22

Isolation of mouse dental pulp-derived Sca-1+PDGFRα+ tissue-committed
stem cells
Ryusuke Nakatsuka, Yoshikazu Matsuoka, Yasushi Uemura,
Yutaka Sasaki, Yoshiaki Sonoda(Department of Stem Cell Biology and Regenerative Medicine, Graduate
School of Medical Science, Kansai Medical University)

P-23

Human SIRPA Polymorphism Modulates Macrophage-MediatedSuppression of Human Hematopoiesis
Takuro Kuriyama1, Katsuto Takenaka1, Koichi Akashi1(1Department of Medicine and Biosystemic Science, School of medicine,
Kyushu University)

P-24

Thrombopoietin controls proliferation of embryonic multipotent
haematopoietic progenitors via JAK2-STAT5
Xin Huang,1, 2 Hiroshi Sakamoto,1, 2 and Minetaro Ogawa1, 2(1 Department of Cell Differentiation, Institute of Molecular Embryology andGenetics, Kumamoto University, 2 Global COE “Cell Fate Regulation Researchand Education Unit,” Kumamoto University)

P-25

Guided differentiation of the mouse induced pluripotent stem cells into
pancreatic cell lineages
Keitaro Yamane, Yuichiro Higuchi, Nobuaki Shiraki, Kazuhiko Kume,
Shoen Kume
(Stem cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto
University)

P-26

Biochemical characterization of the Piwi-subfamily proteins, BmAGO3 and
SIWI, in a BmN4 germ cell line established from silkworm ovaries
Taro Mannen, Mikiko C. Siomi, Haruhiko Siomi(Department of Molecular Biology, Keio University School of Medicine)P-27

Identification and analysis of cancer stem cells in human acutelymphoblastic leukemia
Hiroko Nishida1, Hiroto Yamazaki2, Taketo Yamada3, Satoshi Iwata2,
Takeshi Inukai４, Kanji Sugita ４, Chikao Morimoto2, Yasuo Ikeda1(1 Division of Hematology, Department of Internal Medicine, Keio University
School of Medicine, Tokyo, Japan, 2 Division of Clinical Immunology, Advanced
Clinical Research Center, Institute of Medical Science, University of Tokyo,
Tokyo, Japan,3 Department of Pathology, Keio University, School of Medicine,Tokyo, Japan,４ Department of Pediatrics, School of Medicine, University of
Yamanashi, Yamanashi, Japan)

P-28

Isolation and Identification of Mesenchymal Stem Cells in Human Bone
Marrow
Yo Mabuchi1, Satoru Morikawa1, 2, Hideyuki Okano1, Yumi Matsuzaki1(1 Department of Physiology, Keio University School of Medicine, 2 Departmentof Dentistry and Oral Surgery, Keio University School of Medicine)

P-29

Survival of early neural stem/progenitor cells
Seiji Ishii1, Yohei Okada1, 2, Toshihiko Kadoya3, Takuya Shimazaki1 and
Hideyuki Okano1(1Department of Physiology, School of medicine, Keio University, 2Departmentof Neurology, Graduate school of medicine, Nagoya University, 3CMC R&D
Laboratories, Kyowa Hakko Kirin Company, Limited)

P-30

Abstract Withdrawn

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P-31

Drosophila optic neuron progenitors turn their epithelial structure into
spherical one along with the progression of cell dividing cycle
Minako Orihara-Ono1, Hideyuki Okano1 and Keiko Nakao2(1Department of Physiology, School of Medicine, Keio University, 2Departmentof Physiology, Saitama Medical University)

P-32

Galectin-1: The negative regulator of proliferation of neural precursor cells
in the adult hippocampus
Yoichi Imaizumi1, 2, Masanori Sakaguchi3, Tsuyoshi Morishita4,
Mamoru Ito5, Francoise Poirier6, Kazunobu Sawamoto7, Hideyuki Okano1, 2(1 Institution:Department of Physiology, Keio University, School of Medicine,Tokyo, Japan, 2 Institution:Bridgestone Laboratory of Developmental andRegenerative Neurobiology, Keio University, Tokyo, Japan, 3 Institution:The
Hospital for Sick Children Research Institute, Toronto, Canada, 4Institution:Kyowa Hakko Kogyo Co., Ltd, 5 Institution:Central insititute forexperimental animals, 6 Institution:University of Paris 6th and Paris 7th,
Paris, France, 7 Institution:Nagoya City University Graduate School of Medical
Sciences Aichi, Japan)

P-33

Elucidation of mechanism of functional recovery after transplantation of
NSPCs to SCI
Akimasa Yasuda1,2, Osahiko Tsuji1, Narihito Nagoshi1, Kanehiro Fujiyoshi1,
Kazuya Kitamura1, Masahiko Mukaino3, Takehiko Takagi1, 2,
Yuichiro Takahashi1, 2, Satoshi Nori1, 2, Yoshiaki Toyama1,
Masaya Nakamura1, Hideyuki Okano2(1Department of Orthopaedic surgery, 2Department of Physiology, 3Departmentof Rehabilitation medicine, School of medicine, Keio University, Tokyo)

P-34

Diffusion tensor tractography of peripheral nerve after contusive injury
Takehiko Takagi1,2, Masaya Nakamura1, Masayuki Yamada3,
Keigo Hikishima3, Suketaka Momoshima4, Kanehiro Fujiyoshi1,2,
Shinsuke Shibata2, Hirotaka James Okano2, Yoshiaki Toyama1, andHideyuki Okano2(Departments of 1Orthopaedic Surgery, 2Physiology, and 4Diagnostic Radiology
and 4Physiology, Keio University School of Medicine. 3Central Institute forExperimental Animals.)

P-35

A contribution of Musashi1 to the regulation by Lin-28 in blocking miRNAprocessing
Hironori Kawahara1, Takao Imai1, Hideyuki Okano1（1 Department of Physiology, School of Medicine, Keio University ）P-36

Sox21, a regulator of adult neurogenesis in mouse hippocampus
Satoru Matsuda1,4, Hirotaka James Okano1, Shuichi Tsutsumi2,
Hiroyuki Aburatani2, Yumiko Saga3, Hachiro Sugimoto4, and Hideyuki Okano1(1Dept. Physiol., Keio Univ., Sch. Med., 2Genome Sci. Div., RCAST, Univ. Tokyo,
3Div. Mamm. Dev., Natl. Inst. Genet., 4Dept. Neurosci. Drug Discov,. Kyoto
Univ. Grad. Sch.)

P-37

Visualization of Neurogenesis with Fluorescent Proteins
Hiroaki Kanki1, Marilia Kimie Shimabukuro1, Yoshikazu M. Saito2,
Yoichi Imaizumi1, Atsushi Miyawaki3, Shigeyoshi Itohara2, andHideyuki Okano1(1 Department of Physiology, Keio University School of Medicine, 2 Laboratory
for Behavioral Genetics, Brain Science Institute (BSI), RIKEN, 3 Laboratory
for Cell Function Dynamics, BSI, RIKEN)

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P-38

RNA Binding Protein Musashi2 for Network Formation of Proprioceptive
Sensory Neuron
Shinsuke Shibata1 Shin-Ichi Sakakibara2 Hidemasa Furue3
Megumu Yoshimura3 Takehiko Takagi4 Rika Ohkuma1 Ken-Ichiro Kuwako1
Hirotaka J Okano1 Hideyuki Okano1(1 Department of Physiology, School of Medicine, Keio University. 2 Departmentof Histology and Neurobiology, School of Medicine, Dokkyo University.
3 Department of Integrative Physiology, Graduate School of Medical Sciences,
Kyushu University. 4 Department of Orthopaedic Surgery, School of Medicine,
Keio University.)

P-39

Abstract Withdrawn

P-40

Gene targeting in the common marmoset embryonic stem cells
Seiji Shiozawa1,2,3, Erika Sasaki2, Yusuke Sotomaru3, Hideyuki Okano1(1 Department of Physiology, School of Medicine, Keio University, 2Marmoset
department, Central Institute for Experimental Animals, 3Natural Science
Center for Basic Research and Development, Hiroshima University)

P-41

Temporal specification of neural stem cells
Hayato Naka1,2, Shiho Nakamura1,2, Takuya Shimazaki1,2 , Hideyuki Okano1,2(1 Department of Physiology, School of Medicine, Keio University,
2 Solution-Oriented Research for Science and Technology, Japan Science andTechnology Agency)

P-42

RNA-binding protein Musashi1 inhibits let-7 miRNA activity
Takao Imai, Hironori Kawahara, Satoshi Kawase, Hideyuki Okano.
(Department of Physiology, School of Medicine, Keio University)

P-43

Derivation and characterization of human pluripotent stem cell-derived
neural stem/progenitor cells
Yohei Okada1, 2, 3, Tomoko Tokura1, 2, Shuta Tomisato1, Kazuhisa Kohda1,
Gen Sobue3, Michisuke Yuzaki1, Hideyuki Okano1(1.Department of Physiology, School of Medicine, Keio University, 2.Kanrinmaru
Project, School of Medicine, Keio University, 3.Department of Neurology,
Graduate School of Medicine, Nagoya University)

P-44

Generation of induced pluripotent stem cell lines from adult marmoset cells
Takuji Maeda1, 2, Hiroko Shimada1, Kazutoshi Takahashi3, Ikuo Tomioka2, 4,
Seiji Shiozawa1, 2, Ryo Oiwa2, Akiko Shimada2, Shinya Yamanaka3,
Erika Sasaki2,4, Hideyuki Okano1(1Department of Physiology, School of Medicine, Keio University, 2Marmoset
Research Department, Laboratory of Applied Developmental Biology, Central
Institute for Experimental Animals, 3Department of Stem Cell Biology, Institutefor Frontier Medical Sciences, Kyoto University, 4Department of Biochemistry &Integrative Medical Biology, School of Medicine, Keio University)

P-45

Regenerated young neurons migrate along the blood vessel scaffold in the
striatum after ischemic injury
Takuro Kojima1, 2, Yuki Hirota2, Masatsugu Ema3, Satoru Takahashi3,
Ichiro Miyoshi2, Hideyuki Okano1, and Kazunobu Sawamoto1, 2(1 Keio Univ Sch of Medicine, Tokyo, JAPAN, 2 Nagoya City Univ Grad Sch Med
Sciences, Nagoya, JAPAN, 3 Univ Tsukuba Grad Sch Medicine, Ibaragi, JAPAN)

P-46

Transplantation of human embryonic stem cell derived-neural
stem/progenitor cells for spinal cord injury in adult NOD/Scid mice
Yuichiro Takahashi1, 2, Yohei Okada2, Gentaro Kumagai3, Osahiko Tsuji1, 2,
Yoshiaki Toyama1, Masaya Nakamura1, Hideyuki Okano2(1Department of Orthopedics Surgery, 2Department of Physiology, School of
Medicine, Keio University, 3Department of Orthopedics Surgery, School of
Medicine, Hirosaki University)

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P-47

Comparative study of different administrations of the neural
stem/progenitor cells for spinal cord injury in mice
Yuichiro Takahashi1, 2, Osahiko Tuji1,2, Chikako Hara2, Gentaro Kumagai3,
Yoshiaki Toyama1, Masaya Nakamura1, Hideyuki Okano2(1Department of Orthopedics Surgery, 2Department of Physiology, School of
Medicine, Keio University, 3Department of Orthopedics Surgery, School of
Medicine, Hirosaki University)

P-48

Transplantation of human iPS cell-derived neurospheres for the treatmentof spinal cord injury in NOD-scid mice
Satoshi Nori1, Yohei Okada2, Masaya Nakamura1, Osahiko Tsuji1,
Yuichiro Takahashi1, Kanehiro Fujiyoshi1, Narihito Nagoshi1,
Kazuya Kitamura1, Masahiko Mukaino3, Akimasa Yasuda1,
Hiroko Shimada1, 2, Yoshiaki Toyama1, Shinya Yamanaka4, Hideyuki Okano2(1Department of Orthopaedics, 2Department of Physiology, 3Department of
Rehabilitation Medicine, Keio University, Tokyo, Japan, 4Department of Stem
Cell Biology, Institute for Frontier Medical Sciences, Kyoto University)

P-49

Analysis of transcriptional regulation of musashi1 gene in neural stem cells
Satoshi Kawase, Takao Imai, Chikako Hara, Hideyuki Okano(Department of Physiology, School of Medicine, Keio University)

P-50

All-trans retinoic acid inhibits the efflux activity of breast cancer resistance protein
Lawrence Lein, Yasuo Nagai, Hideyuki Okano, Yumi Matsuzaki(Department of Physiology, Keio University School of Medicine)

P-51

Analysis of a transcription factor involved in neural stem cell maintenanceShin-ichi Fukami 1, 2, Hideyuki Okano 1(1 Department of Physiology, Keio University School of Medicine, Tokyo, Japan,
2 SORST, JST, Tokyo, Japan)

P-52

Role and Mechanisms of reactive astrocyte’s migration after spinal cord injury
Francois Renault-Mihara1, Masahiko Mukaino2, Masaya Nakamura2,
Yoshiaki Toyama2 and Hideyuki Okano1.
(1Department of Physiology, 2Department of Orthopedic Surgery, Keio
University School of Medicine)

P-53

Stamporation: a microinjection method into cultured cells
Chikako Miyauchi-Hara1,2, Atsushi Miyawaki2, Hideyuki Okano1(1Department of Physiology, School of Medicine, Keio University, 2Laboratory
for Cell Function Dynamics, Brain Science Institute, RIKEN)

P-54

Nurr1 gene induced human NT2N cells provides therapeutic benefits intransplanted MCAO stroke model rats
Koichi Hara1, 2, Noriyuki Matsukawa2, Takao Yasuhara2, Seung U. Kim3,
Takeshi Kawase1, Cesario V. Borlongan2(1Department of Neurosurgery, KEIO university, 2Department of Neurology,
Medical college of Georgia, 3Brain Disease Research Center, Ajou University
School of Medicine)

P-55

Analysis of Musashi1 function in human glioma stem cells and developmentof gene therapy for tumor growth suppression
Jun Muto1.2, Takao Imai2, Yumi Matsuzaki2, Takeshi Kawase1,
Hideyuki Okano2(1Department of Neurosurgery, School of Medicine, Keio University
2 Department of physiology, School of Medicine, Keio University)

7th Stem Cell Research Symposium

10

May 15 (Fri)-16(Sat), 2009

Saturday, May 16.

The Second Day

Session 4：Multipotent Stem Cell
Chair Takashi Yokota

9:00～10:20(Department of Stem Cell Biology, Institute of Medical, Pharmaceutical and
Health Sciences, Kanazawa University)
O-13

Dax1 functions as a negative regulator of Oct3/4 in mouse embryonic stem
cells
Hiroshi Koide1, Chuanhai Sun1, Yuhki Nakatake2, Tadayuki Akagi1,
Hiroki Ura1, Takahiko Matsuda3, Hitoshi Niwa2, Takashi Yokota1(1Department of Stem Cell Biology, Institute of Medical, Pharmaceutical and
Health Sciences, Kanazawa University, 2Laboratory for Pluripotent Cell
Studies, RIKEN Center for Developmental Biology, 3Department of Genetics,
Howard Hughes Medical Institute, Harvard Medical School)

O-14

Differential requirement of nucleostemin for cell viability between ES cells
and neural stem cells
Akihiko Okuda1, Miyuki Katano1, Hidemasa Kato1, Shinji Masui2,
Jun Nomura1(1Division of Developmental Biology, Research Center for Genomic Medicine,
Saitama Medical University, 2Regerative Medicine, Research Institute,International Medical Center of Japan)

O-15

Correction of Duchenne muscular dystrophy in induced pluripotent stem
cells using a human artificial chromosome
Yasuhiro Kazuki1, Masaharu Hiratsuka1, Masato Takiguchi1,
Mitsuhiko Osaki1, Hidetoshi Hoshiya1, Kei Hiramatsu1, Naoyo Kajitani1,
Toko Yoshino1, Kanako Kazuki1, Masato Nakagawa2, Kazutoshi Takahashi2,
Shinya Yamanaka2 and Mitsuo Oshimura1(1Department of Biomedical Science, Institute of Regenerative Medicine andBiofunction, Graduate School of Medical Science, Tottori University,
2Department of Stem Cell Biology, Institute for Frontier Medical Sciences,
Kyoto University)

O-16

Derivation of multipotential hematopoietic progenitors and mature blood
cells from human induced pluripotent stem cells
Feng Ma1, 2, Yasuhiro Ebihara1, Hiromitsu Nakauchi2 and Kohichiro Tsuji1, 2(1Division of Cellular Therapy, Advanced Clinical Research Center, 2Division ofStem Cell Therapy, Center for Stem Cell and Regenerative Medicine;
Institute of Medical Science, The University of Tokyo)

Coffee Break

10:20～10:40

Session 5：Cancer Stem Cell
Chair Toru Nakano

10:40～11:20(Department of Pathology, Osaka University Medical School)
O-17

Evi-1 is up-regulated by MLL oncoproteins selectively in hematopoietic stem
cells
Shunya Arai, Susumu Goyama, Munetake Shimabe, Motoshi Ichikawa,
Yoichi Imai, Mineo Kurokawa(Department of Hematology and Oncology, Graduate School of Medicine, theUniversity of Tokyo)

11

7th Stem Cell Research Symposium

May 15 (Fri)-16(Sat), 2009

O-18

Hes1 rapidly induces blastic crisis in CML by conferring committedprogenitors with self-renewal capabilities.
Fumio Nakahara1, 3, Jiro Kitaura1, Mamiko Sakata-Yanagimoto2,
Yukiko Komeno1, Naoko Kato1, Mineo Kurokawa3, Shigeru Chiba2,
Toshio Kitamura1(1Division of Cellular Therapy, The Institute of Medical Science, The
University of Tokyo, 2Department of Hematology, University of Tsukuba,
3Department of Hematology-Oncology, University of Tokyo)

Special Lecture 4

11:20～12:00

Hemopoietic Stem Cells and their Niche
SK Nilsson（Australian Stem Cell Centre, Melbourne, Australia）

Poster Session

12:00～14:00

Gallery B：lunch served

Session 6：New Aspect of Stem Cell research
Chair Toshio Suda

14:00～15:20(Department of Cell Differentiation, Keio University School of Medicine)
O-19

Hair follicle stem cells provide a COL17A1-dependent niche for melanocyte
stem cells
Shintaro Tanimura1, 2, ＊, Yuko Tadokoro1, ＊, Ken Inomata1, Wataru
Nishie2, James R. McMillan2, Daisuke Sawamura2, Hiroshi Shimizu2,
Emi K. Nishimura1, 3(1Department of Stem Cell Medicine, Cancer Research Institute, Kanazawa
University, 2Department of Dermatology, Hokkaido University Graduate
School of Medicine, 3Department of Stem Cell Biology, Medical ResearchInstitute, Tokyo Medical and Dental University, ＊equally contribution)

O-20

Generation of pancreas and functional islets from pluripotent stem cells by
way of blastocyst complementation
Toshihiro Kobayashi1, 2, Hiromitsu Nakauchi1, 2(1Division of Stem Cell Therapy, Center for Stem Cell and Regenerative Medicine,
Institute of Medical Science, University of Tokyo, 2Japan Science Technology
Agency, ERATO, Nakauchi Stem Cell and Organ Regeneration Project)

O-21

Identification of human cartilage progenitor cells in the perichondrium ofauricular cartilage
Takanori Takebe1, Shinji Kobayashi1, 2, Midori Inui1, Yuki Emoto1,
Yasuharu Ueno1,Yun-Wen Zheng1, Jiro Maegawa2, Hideki Taniguchi1(1Department of Regenerative Medicine; 2Department of Plastic andReconstructive Surgery, Yokohama City University School of Medicine, Japan)

O-22

Mastermind-1/Mastermind-2 are essential components of Notch signaling
pathways
Toshinao Oyama1, Kenichi Harigaya1, Nobuo Sasaki2, Yoshiaki Okamura2,
Yumiko Saga2, Katsuto Hozumi3, Reiko Sakamoto4, Mitsuharu Sato4,
Nobuaki Yoshida4, and Motoo Kitagawa1(1 Department of Molecular and Tumor Pathology, Chiba University Graduate
School of Medicine, 2 Division of Mammalian Development, National Instituteof Genetics, 3 Department of Immunology, Tokai University School of Medicine,
4 Institute of Medical Science, University of Tokyo)

Coffee Break7th Stem Cell Research Symposium

15:20～15:40

12

May 15 (Fri)-16(Sat), 2009

Session 7：Network of Transcription Factor and Epigenetics
15:40～17:00
Chair Haruhiko Siomi(Department of Molecular Biology, School of Medicine, Keio University)
O-23

Establishment and Effects of Epigenetic Pattern Regulated by a Tissue
Specific Transcription Factor
Kenji Kitajima, Jie Zheng, Toru Nakano(Department of Pathology, Osaka University Medical School)

O-24

Piwi regulation by a large Maf transcription factor, traffic jam, in
Drosophila ovarian somatic cells
Sachi Inagaki1, Mikiko Siomi1, and Haruhiko Siomi1(1 Department of Molecular Biology, School of Medicine, Keio University)

O-25

A systems approach reveals myogenesis genome network is regulated by thetranscriptional repressor RP58
Shigetoshi Yokoyama1, Yoshiaki Ito1, Hiroshi Asahara1(1Department of Systems BioMedicine, National Research Institute for Child
Health and Development)

O-26

Role of Polycomb group protein Ring1B and its mediated histone H2A
ubiquitylation in mouse ES cell maintenance
Mitsuhiro Endoh1, Takaho A. Endo2, Haruhiko Koseki1(1 RIKEN Research Center for Allergy and Immunology, 1-7-22 Suehiro,
Tsurumi-ku, Yokohama 230-0045, Japan, 2 RIKEN Genomic Sciences Center,
1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan)

Special Lecture 5

17:00～17:40

Translational Regulation in Germline Stem Cell Self-Renewal
Haifan Lin（Yale Stem Cell Center, and Departments of Cell Biology and Genetics,
Yale University School of Medicine, New Haven CT 06511, USA.）

Closing Remarks

Next Director

Koichi Akashi

17:40～17:50(Department of Medicine and Biosystemic Science,
School of medicine, Kyushu University)

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7th Stem Cell Research Symposium

May 15 (Fri)-16(Sat), 2009

Poster Session

Saturday, May 16

12:00～14:00

Gallery B
P-56

Transcriptional Mediator in niche cells plays a role to support hematopoietic
stem/progenitor cells
Akiko Sumitomo1, Kana Inoue1, Ruri Ishino1, Kenji Yonezawa1,
Norinaga Urahama1 and Mitsuhiro Ito1, 2(1 Laboratory of Hematology, Division of Medical Biophysics, Kobe University
Graduate School of Health Sciences, 2 Division of Hematology, Department ofMedicine, Kobe University Graduate School of Medicine)

P-57

Gone early maintains undifferentiated state of primordial germ cells andregulates establishment of germline stem cells in Drosophila ovary
Shinya Matsuoka1, 2, Miho Asaoka1, 2, Yasushi Hiromi1, 2(1Department of Developmental Genetics, National Institute of Genetics,
2Department of Genetics, Sokendai)

P-58

Histone acetyltransferases MOZ and MORF are essential for self-renewal ofhematopoietic stem cells
Takuo Katsumoto, Issay Kitabayashi(Division of Molecular Oncology, National Cancer Center Research Institute)P-59

A system to express multiple genes by a single vector
Shinji Masui(Division of Molecular Biology and Cell Engineering, Department ofRegenerative Medicine, Research Institute, International Medical Center ofJapan)

P-60

The maintenance of undifferentiated state of stem-cell precursors in
Drosophila germline
Miho Asaoka1, 2, Shinya Matsuoka1 and Yasushi Hiromi1(1Department of Developmental Genetics, National Institute of Genetics,
2Department of Genetics, Sokendai)

P-61

Age-related changes in prospectively isolated muscle satellite cells
Ikemoto-Uezumi M1, Uezumi A2, Tsuchida K2, Fukada S3,Hashimoto N1(1Department of Regenerative Medicine, NILS, National Center for Geriatrics
and Gerontology, 2Division for Therapies against Intractable Diseases, ICMS,
Fujita Health University, 3Department of Immunology, Graduate School of
Pharmaceutical Sciences, Osaka University)

P-62

Generating Induced Pluripotent Stem Cells from Common Marmoset Bone
Marrow Cells
Ikuo Tomioka1, 2, Takuji Maeda1, 2, Akiko Shimada1, 3, Ryo Ooiwa1, 3,
Hideyuki Okano2, Erika Sasaki1, 2(1Central Institute for Experimental Animal, Kanagawa, Japan,
University, School of Medicine Tokyo, Japan, 3JAC Inc., Tokyo, Japan)

P-63

2Keio

Generation of functional T cells from ES cells and induced pluripotent stem
(iPS) cells in vitro
Haruka Wada and Ken-ichiro Seino(Division of Bioregulation research, Institute of Medical Science, St. Marianna
University School of Medicine)

P-64

Long-term repopulation and lymphoid differentiation potential of theembryonic hemangioblast
Mitsujiro Osawa1 and Michael Kyba2(1 Department of Cellular and Molecular Medicine, Graduate School of
Medicine, Chiba University, 2 Department of Pediatrics, Division of
Hematology-Oncology/Blood and Bone Marrow Transplantation, University of
Minnesota)

7th Stem Cell Research Symposium

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May 15 (Fri)-16(Sat), 2009P-65

A novel Flk1 enhancer for hemato-cardiovascular progenitor
Hiroyuki Ishitobi, Asami Wakamatsu, Michito Hamada, Satoru Takahashi,
Masatsugu Ema(Univ. Tsukuba, Grad. Sch. Comprehensive Human Sci., Dept. Anatomy
Embryol.)

P-66

Genotoxic stress abrogates renewal of melanocyte stem cells by triggering
their differentiation
Ken Inomata1, 2, Takahiro Aoto1, 6, Nguyen Thanh Binh1,
Natsuko Okamoto1, Shintaro Tanimura1, 3, Tomohiko Wakayama4,
Shoichi Iseki4, Eiji Hara5, Takuji Masunaga2, Hiroshi Shimizu3, Emi K.
Nishimura1, 6(1 Division of Stem Cell Medicine, Center for Cancer and Stem Cell Research,
Cancer Research Institute, Kanazawa University, 2 Fundamental ResearchLaboratories, KOSÉ Corporation, 3 Department of Histology and Embryology,
Graduate School of Medical Science, Kanazawa University, 4 Cancer Instituteof Japanese Foundation for Cancer Research, 5 Department of Dermatology,
Hokkaido University Graduate School of Medicine, 6 Department of Stem Cell
Biology, Medical Research Institute, Tokyo Medical and Dental University)

P-67

Characterization of cells that have hematopoietic activity in the placenta ofmouse embryo
Gomaa, A.,1, 2 Nobuhisa, I.,1 Yamasaki, S.,1 Taga, T.1, 2, 3
(1Dept. of Cell Fate Modulation, Institute of Molecular Embryology andGenetics, Kumamoto Univ., 2GCOE Kumamoto Univ. Kumamoto, Japan, 3Dept.
of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and
Dental Univ.)

P-68

Cyclin D1 inhibits astrocyte differentiation from neural stem/progenitor
cells in a manner independent of cell cycle regulation
Norihisa Bizen1, Toshihiro Inoue2, Takeshi Shimizu3, Tetsushi Kagawa1,
Tetsuya Taga1(1Department of Stem Cell Regulation, Medical Research Institute, Tokyo
Medical and Dental University, 2Department of Ophthalmology and Visual
Science, Graduate School of Medical Sciences, Kumamoto University,
Laboratory for Vertebrate Axis Formation, RIKEN Center for Developmental
Biology)

P-69

Formation of functional islets with distinct 3D structure in vitro
Hiroki Saito1,2, Masaki Takeuchi1, Kaori Suzuki1, Kazuhiro Chida2,
and Atsushi Miyajima1(1Institute of Molecular and Cellular Biosciences, 2Graduate School of
Agricultural and Life Sciences, The University of Tokyo)

P-70

MT1-MMP is required for hematopoietic maturation in the BM niche
Chiemi Nishida1, Beate Heissig1, Daigo Niiya3, Motoharu Seiki3,
Koichi Hattori1(1Division of Stem Cell Regulation, 1.2 Frontier Research Initiative,
1.2,3 Institute of Medical Science, University of Tokyo, 3 Division of Cancer Cell
Research, Institute of Medical Science, University of Tokyo)

P-71

FRS2α regulates Erk levels to control a self-renewal target Hes1 andproliferation of FGF-responsive neural stem/progenitor cells
Takuya Sato1, Takuya Shimazaki2, Hayato Naka2, Hideyuki Okano2,
and Noriko Gotoh1(1Division of Systems Biomedical Technology and 2Department of Physiology,
Keio University School of Medicine, Tokyo, Japan.)

P-72

Evi-1 up-regulates Pbx1 expression in hematopoietic stem/progenitors andleukemic cells.
Munetake Shimabe, Susumu Goyama, Motoshi Ichikawa, Yoichi Imai,
Tsuyoshi Takahashi, Akira Hangaishi, Mineo Kurokawa(Department of Hematology & Oncology, Graduate School of Medicine,
University of Tokyo)

15

7th Stem Cell Research Symposium

May 15 (Fri)-16(Sat), 2009P-73

Wnt/beta-catenin signals in hepatic differentiation of human mesenchymal
stem cells: its relation with E-cadherin
Yoshiko Hoshikawa1, Yoshiaki Matsumi1, Yuta Tezuka1, Hideharu Okamoto1,
An AFIDA ASHLA1, Hiroyuki Tsuchiya1, Akihiro Umezawa2, Goshi Shiota1(1Division of Molecular and Genetic Medicine, Department of Genetic
Medicine and Regenerative Therapeutics, Tottori University, 2Department of
Reproductive Biology and Pathology, National Research Institute for Child
Health and Development)

P-74

Properties of cardiac pacemaker like cells isolated from mouse ES cells
Keita Arakawa, Shinichi Itoh, Natsumi Shimizu, Kumi Morikawa,
Yasuaki Shirayoshi, Ichiro Hisatome.
(Division of Regenerative Medicine and Therapeutics, Department of Genetic
Medicine and Regenerative Therapeutics, Graduate School of Medical Science,
Tottori University)

P-75

Characterization of human iPS cells established from Down syndrome
patient-derived fibroblast cell lines
Masaharu Hiratsuka1, Narumi Uno2, Natsuko Imaoka2, Shigeko Masuda2,
Yasuhiro Kazuki2, Mitsuhiko Osaki2, Katsumi Higaki3, Kazutoshi Takahashi4,
Shinya Yamanaka4 and Mitsuo Oshimura1, 2(1 Division of Molecular and Cell Genetics, Department of Molecular andCellular Biology, School of Life Sciences, Faculty of Medicine, 2 Department of
Biomedical Science, Institute of Regenerative Medicine and Biofunction,
Graduate School of Medical Science, 3 Division of Functional Genomics,
Research Center for Bioscience and Technology, Tottori University,
4 Department of Stem Cell Biology, Institute for Frontier Medical Sciences,
Kyoto University)

P-76

Recovery of human dystrophin expression in the mdx mesoangioblast-derived
skeletal muscle using the human artificial chromosome (HAC)
Hidetoshi Hoshiya1, Yasuhiro Kazuki1, Saverio Tedesci2, Satoshi Abe1,
Masato Takiguchi1, Naoyo Kajitani1, Yoshinori Watanabe1, Toko Yoshino3,
Yasuaki Shirayoshi4, Katsumi Higaki3, Graziella Messina2,
Giulio Cossu2 and Mitsuo Oshimura1(1Department of Biomedical Science, Graduate School of Medical Science,
Tottori University, 2Stem Cell Research Institute, San Raffaele Scientific
Institute, Milan, Italy, 3Research Center for Bioscience and Technology,
Tottori University, 4Department of Genetic Medicine and Regenerative
Therapeutics, Graduate School of Medical Science, Tottori University)

P-77

Towards extension of life-span of a human mesenchymal stem cells using ahuman artificial chromosome carrying a conditional centromere
Yuichi Iida1, Jun-ichirou Ohzeki2, Yasuhiro Kazuki1, Hidetoshi Hoshiya1,
Masato Takiguchi1, Masahiro Hayashi1, Megumi Nakano3,
Hiroshi Masumoto2, William C. Earnshaw4, Vladimir Larionov3 andMitsuo Oshimura1(1 Department of Biomedical Science, Institute of Regenerative Medicine andBiofunction, Graduate School of Medical Sciences, Tottori University, Yonago,
Tottori, Japan, 2 Institute of Kazusa DNA reserch, Kazusa, Chiba, Japan,
3Laboratory of Biosystems and Cancer, National Cancer Institute, NIH,
Bethesda, Maryland, United States, 4 Wellcome Trust Centre for Cell Biology,
University of Edinburgh, scotland, United Kingdom.)

7th Stem Cell Research Symposium

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May 15 (Fri)-16(Sat), 2009

P-78

Human FVIII Expression Using a HAC Vector Toward Stem Cell- MediatedGene Therapy for Hemophilia A
Hajime Kurosaki1, Narumi Uno1, Masaharu Hiratsuka2, Yuichi Iida1,
Yasuhiro Kazuki1, Chie Ishihara3, Yuna Yakura3, Hiroyuki Takeya3
and Mitsuo Oshimura1.
(1Department of Biomedical Science, Institute of Regenerative Medicine andBiofunction, Graduate School of Medical Science, Tottori University, 2Divisionof Human Genome Science, Department of Molecular and Cellular Biology,
School of Life Sciences, Faculty of Medicine, Tottori University, and 3Divisionof Pathological Biochemistry, Department of Biomedical Sciences, School ofLife Sciences, Faculty of Medicine, Tottori University )P-79

Evaluation of BCR-ABL-positive leukemia stem cells with imatinib-therapy
and apoptosis induced by bortezomib-treatment
Yosuke Minami1, Akihiro Abe1, Yachiyo Kuwatsuka1, Yuka Nomura1,
Ryohei Tanizaki1, Tomoki Naoe1(1Department of Hematology and Oncology, Nagoya University Graduate
School of Medicine)

P-80

Cyclin C Regulates Quiescence of Human Hematopoietic Primitive
Progenitor Cells
Yasuhiko Miyata1, 2, Vladimir Jankovic2, Tomoki Naoe1, Stephen Nimer2(1Hematology and Oncology Department, Nagoya University Graduate Schoolof Medicine, 2Molecular Pharmacology and Chemistry Program, Memorial
Sloan-Kettering Cancer Center)

P-81

Molecular roles for HOX gene products and the fusion with NUP98 inhematopoieis and lukemogenesis
Yoshinori Ohno, Shin’ichiro Yasunaga, Motoaki Ohtsubo,
Yuka Kageyama, Rie Tokimoto, Yoshihiro Takihara(Dept. Stem Cell Biol., RIRBM, Hiroshima Univ.)

P-82

Role of Scmh1 in the regulation of Polycomb-group complex 1, which acts as
the E3 ubiquitin ligase for histone H2A and Geminin
Motoaki Ohtsubo, Shin'ichiro Yasunaga, Miyuki Tsumura, Yoshinori Ohno,
Yuka Kageyama, Shunsuke Kimura, Rie Tokimoto, Yoshihiro Takihara(Dept. Stem Cell Biol., RIRBM, Hiroshima Univ.)

P-83

Mesenchymal progenitors distinct from muscle satellite cells contribute toectopic fat formation in skeletal muscle
Akiyoshi Uezumi1, So-ichiro Fukada2, Harumoto Yamada3, Ichizo Nishino4,
Shin’ichi Takeda5, and Kunihiro Tsuchida1(1Division for Therapies against Intractable Diseases, Institute forComprehensive Medical Science, Fujita Health University, 2Department of
Immunology, Graduate School of Pharmaceutical Sciences, Osaka University,
3Department of Orthopaedic Surgery, Fujita Health University, 4Departmentof Neuromuscular Research, National Institute of Neuroscience, National
Center of Neurology and Psychiatry (NCNP), 5Department of Molecular
Therapy, National Institute of Neuroscience, NCNP)

P-84

Purification of hematopoietic stem cells without negative-selection
Yasushi Kubota1, Ivo Lieberam2, Thomas M. Jessell2,
Shin-ichi Nishikawa1(1Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology,
2Howard Hughes Medical Institute, Department of Biochemistry andMolecular Biophysics, Center for Neurobiology and Behavior, Columbia
University)

17

7th Stem Cell Research Symposium

May 15 (Fri)-16(Sat), 2009

P-85

Molecular mechanisms of primitive endoderm specification during thepreimplantation mouse development
Julien BOUISSAC, Naoko YOSHIOKA, Lars M. JAKT &Shin-Ichi NISHIKAWA(Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology,
Kobe)

P-86

iPS technology as a new tool to understand the relationship between stem
cell and cancer
Kenichiro Kobayashi, Satomi Nishikawa, Shin-ichi Nishikawa(Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology)

P-87

Investigation of lymphoid potential in the early VE-cadherin positive
population
Yosuke Tanaka, Shin-ichi Nishikawa(RIKEN Kobe Institute Center for Developmental Biology, Laboratory for
Stem Cell Biology)

P-88

Adult pluripotent cells for personalized therapy ?
Hervé Le Mouellic, Shin-Ichi Nishikawa

P-89

Analysis of epigenetic systems maintaining pluripotency
Kazuhiro Murakami, Hitoshi Niwa
(Laboratory for Pluripotent Cell Studies, Center for Developmental Biology,
RIKEN)

P-90

Generation and propagation of mouse neural crest progenitor cells
Jitsutaro Kawaguchi1, 2, Jennifer Nichols1 and Austin Smith1(1 Wellcome Trust Centre for Stem Cell Research, University of Cambridge,
Cambridge,2 Current Address: Center for Developmental Biology, Riken, Kobe)

P-91

Germline transmission of a novel human artificial chromosome (14HAC)
vectorMinoru Kakeda1, Keiko Nagata1, Akiyo Okazaki1, Mitsuo Oshimura2,
Kazuma Tomizuka1（1 Innovative Drug Research Laboratories, Research Division, Kyowa Hakko
Kirin Co., Ltd., 2 Graduate School of Medical Science, Tottori University）

P-92

Molecular function of Tsukushi in the brain
Ayako Ito 1, 2, Yohei Shinmyo1, Masahiro Yamaguchi3, Rika Nakayama4,
Naoko Oshima4, Hideaki Tanaka1,2, Kunimasa Ohta1(1Department of Developmental Neurobiology, Graduate School of Medical
Sciences, Kumamoto University, 2Global COE, Kumamoto University,
3Graduate School of Medical science University of Tokyo, 4RIKEN CDB)

P-93

Highly efficient transient gene expression and gene targeting in humanpluripotent stem cells with helper-dependent adenoviral vectors
Keiichiro Suzuki1, Kaoru Mitsui1, Emi Aizawa1, Kouichi Hasegawa2,
Eihachiro Kawase3, Hirofumi Suemori2, Norio Nakatsuji3, 4 andKohnosuke Mitani1(1Gene Therapy Division, Research Center for Genomic Medicine, Saitama
Medical University. 2Laboratory of Embryonic Stem Cell Research, Stem Cell
Research Center and 3Department of Development and Differentiation,
Institute for Frontier Medical Sciences, and 4Institute for Integrated
Cell-Material Sciences, Kyoto University.)

P-94

Acute myelogenous leukemia blasts convert into adherent myofibroblast
cells
Ryosuke Shirasaki, Haruko Tashiro, Yoko Oka, Toshihiko Sugao,
Nobu Akiyama, Kazuo Kawasugi and Naoki Shirafuji(Department of Hematology / Oncology, Teikyo University School of Medicine
2-11-1, Kaga, Itabashi-ku, Tokyo 173-8606 Japan)

7th Stem Cell Research Symposium

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May 15 (Fri)-16(Sat), 2009P-95

The role of polycomb group gene Ring1B in the proliferation of hepatic stem
cells
Takako Naito1, Yasuharu Ueno1, Yun-Wen Zheng1, Kyoichi Isono Isono2,
Haruhiko Koseki2, Hideki Taniguchi1(1Dept. of Regenerative Medicine, Graduate School of Medicine, Yokohama
City University, 2 Laboratory for Developmental Genetics, RIKEN ResearchCenter for Allergy and Immunology)

P-96

The prospective identification and differentiation of human hepatic stem
cells
Bin Li, Yun-Wen Zheng, Yousuke Miyabe, Yasuharu Ueno and
Hideki Taniguchi(Dept. of Regenerative Med., Grad. Sch. of Med., Yokohama City Uni., Japan)

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7th Stem Cell Research Symposium