May 13 (Thu)-15(Sat), 2010

The 8th Stem Cell Research Symposium
Program

Date： May 13 (Thu) - 15(Sat), 2010

Venue

：The Awaji Yumebutai
International Conference Center

Director ：Koichi Akashi
Department of Medicine and Biosystemic Science,
Graduate School of Medical Sciences, Kyushu University

Organizer ：Stem Cell Research Symposium
Cosponsor：Kyushu University Global COE Program
Cell-fate Decision:Function and Dysfunction in Homeostasis

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

Thursday, May 13.

The First Day

Registration・Exhibit posters
Opening Remarks

Director Koichi Akashi

12:00～
13:20～13:30(Department of Medicine and Biosystemic Science, Graduate School of
Medical Sciences, Kyushu University)

Session 1：Neural stem cell

13:30～14:50

Chair Tetsuya Taga
(Department of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and
Dental University)

O-1

Neurogenesis-to-gliogenesis switch of neural stem cells
Hayato Naka-Kaneda1, Shiho Nakamura1, Takuya Shimazaki1, Hideyuki
Okano1(1 Department of Physiology, School of Medicine, Keio University)

O-2

Roles of p57 (kip2) in acquirement and maintenance of adult mouse neural stem
cell quiescence
Shohei Furutachi1, Yusuke Hirabayashi1, Akinobu Matsumoto2, Keiichi
Nakayama2 and Yukiko Gotoh1(1 Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo,
Japan 2 Department of Molecular and Cellular Biology, Medical Institute of
Bioregulation, Kyushu University, Fukuoka, Japan)

O-3

Tsukushi is a Frizzled ligand that regulates the proliferation of neuronal
stem/progenitor cells
Kunimasa Ohta1, Ayako Ito1,2, Yohei Shinmyo1, Naoko Kaneko3, Yuki Hirota3,
Masahiro Yamaguchi4, Kazunobu Sawamoto3, Hideaki Tanaka1,2(1Graduate School of Medical Sciences, Kumamoto University, 2Global COE,
Kumamoto University, Institute of Molecular Medicine, 3Nagoya City
University Graduate School of Medical Sciences, 4Graduate School of Medical
science, University of Tokyo)

O-4

shRNA library screening reveals a novel mechanism involved in oligodendrocyte
differentiation
Nobuko Tsuchida-Straeten1, Keiko Miwa1, Yuki Kujuro and Toru Kondo1(1Laboratory for Cell Lineage Modulation, Center for Developmental Biology,
RIKEN, Kobe 650-0047, Japan)

Coffee Break

14:50～15:10

Session 2：Hematopoietic stem cell ( I )15:10～16:50

Chair Toshio Kitamura(Division of Stem cell signaling, Stem cell Therapy Center, Institute of Medical Science,
University of Tokyo)

O-5

High resolution purification and functional characterization of primitive human
cord blood-derived CD34–negative SRCs
Yutaka Sasaki, Mari Murakami, Yoshikazu Matsuoka, Masaya Takahashi,
8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

Ryusuke Nakatsuka, Yasushi Uemura, Yoshiaki Sonoda(Department of Stem Cell Biology and Regenerative Medicine, Graduate Schoolof MedicalScience, Kansai Medical University)

O-6

Prospective isolation and functional characterization of human hematopoietic
stem cell-supportive mesenchymal stromal cells
Yoshikazu Matsuoka1,2, Yutaka Sasaki1, Masaya Takahashi1, Ryusuke
Nakatsuka1, Yasushi Uemura1, Yoshiaki Sonoda1(1Department of Stem Cell Biology and Regenerative Medicine, Graduate Schoolof Medical Science, Kansai Medical University, 2Depertment of Cancer
Immunology, Graduate School of Medicine, Osaka University)

O-7

A new-type Sendai virus vector expressing HoxB4 allows safe and efficient
expansion of human HSC in a sheep in utero transplant model
Shigeo Masuda1, Tomoyuki Abe1-3, Hiroshi Ban4,Satoshi Hayashi5,Hironori
Takahashi1,5,Makoto Inoue4, Mamoru Hasegawa4, Yoshikazu Nagao2,3, Yutaka
Hanazono1(1 Division of Regenerative Medicine, Center for Molecular Medicine, Jichi
Medical University, 2 Department of Agriculture, Utsunomiya University, 3
United Graduate School of Agricultural Science, Tokyo University of Agriculture
and Technology, 4 DNAVEC Corporation, 5 Department of Obstetrics andGynecology, National Center for Child Health and Development)

Ex vivo enhancement of repopulating and self-renewal potentials in

O-8

hematopoietic stem cells by a minimal combination of cytokines
Jun Ooehara, Hiromitsu Nakauchi, Hideo Ema(Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative
Medicine, Institute of Medical Science, University of Tokyo)

O-9

Requirement of Etv-2 for the specification of the hemangioblastic mesoderm
Hiroshi Kataoka(Stem Cell Biology RIKEN CDB)

Coffee Break

16:50～17:10

Special Lecture 1
Chair Toshio Kitamura

17:10～18:10

Molecular functions of cyclins in mouse development
Peter Sicinski, M.D. Ph.D.
（Department of Cancer Biology, Dana-Farber Cancer Institute andDepartment of Pathology, Harvard Medical School）

Poster Session

18:30～

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

Friday, May 14.

The Second Day

Session 3：Multipotent stem cell ( I )Chair

8:30～9:30

Atsushi Iwama(Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba
University

O-10

Long-term repopulation of adult hematopoiesis by in vitro differentiated blood
cells from ESCs/iPSCs by LIM homeobox transcription factor, Lhx2
Kenji Kitajima1, Jie Zheng2, Ken-ichi Minehata1, Toru Nakano3, Takahiko Hara1(1Stem Cell Project, The Tokyo Metropolitan Institute of Medical Science,
2Molecular & Cellular Biology, Sunnybrook Health Sciences Centre, Universityof Toronto, 3Graduate School of Frontier Biosciences, Osaka University)

O-11

Linkage between histone modification and DNA methylation in early
embryogenesis
Toshinobu Nakamura1, Yu-Jung Liu2, Hiroyuki Nakashima2, Makoto
Tachibana3, Yoichi Shinkai3, Toru Nakano1,2（ 1 Graduate School of Medicine, 2 Graduate School of Frontier Biosciences,
Osaka University, 3 Institute for Virus Research, Kyoto University）

O-12

Guidance of monoclonal T lineage lymphocytes from human induced pluripotent
stem cells originating from a single peripheral T lymphocyte
Toshinobu Nishimura, Shin Kaneko, Haruo Gotoh, Hiromitsu Nakauchi(Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative
Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo,
Japan)

Coffee Break

9:30～9:50

Session 4：Multipotent stem cell ( II )9:50～10:50

Chair

Shigeru Chiba(Department of Clinical and Experimental Hematology,
Comprehensive Human Sciences, University of Tsukuba)

O-13

Graduate

SchoolofHigh efficient experimental model of reprogramming in primordial germ cells
leads to the prospective analysis in generation of pluripotency
Takeo Kosaka1, Go Nagamatsu2, Keiyo Takubo2, Mototsugu Oya1, Toshio Suda2(1 Department of Urology, 2 The Sakaguchi Laboratory of Developmental
Biology, Keio University School of Medicine)O-14

A novel in vivo differentiation system of transplantable HSCs from iPSCs
through teratoma formation
Nao Suzuki1,2, Satoshi Yamazaki1, Tomoyuki Yamaguchi1,2, Hiromitsu
Nakauchi 1,2(1 Division of Stem Cell Therapy, Center for Stem Cell Biology andRegenerative Medicine, Institute of Medical Science, University of Tokyo, 2
Nakauchi Stem Cell and Organ Regeneration Project, ERATO, JST)

O-15

Sulfation contributes to the maintenance of mouse embryonic stem cells, thedifferentiation of the embryoid body and the progression of neurogenesis
Norihiko Sasaki1, Takuya Hirano1, Tomomi Ichimiya1, Masahiro Wakao2,
Kazumi Hirano1, Akiko Kinoshita-Toyoda3, Hidenao Toyoda3, Yasuo Suda2,
Shoko Nishihara1(1 Laboratory of Cell Biology, Department of Bioinformatics, Faculty ofEngineering, Soka University, 2 Department of Nanostructure and Advanced

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010Materials, Graduate School of Science and Engineering, Kagoshima University,
3 Laboratory of Bio-analytical Chemistry, College of Pharmaceutical Sciences,
Ritsumeikan University)

Coffee Break

10:50～11:10

Special Lecture 2
Chair Shigeru Chiba

11:10～12:10

Factors modulating reprogramming somatic cells topluripotent Stem cells
Bing Lim, M.D. Ph.D
（Genome Institute of Singapore）

Lunch

12:10～13:40
Secretary society

Room304

Session 5：Cancer stem cell ( I )Chair

12:20～13:20

13:40～15:00

Atsushi Hirao(Division of Molecular Genetics, Cancer Research Institute, Kanazawa University)

O-16

Defined factors induce reprogramming of gastrointestinal cancer cells
Hiromitsu Hoshino 1, Hideshi Ishii 1, 2, Norikatsu Miyoshi 1, Ken-ichi Nagai 1,
Dyah Laksmi Dewi 1, Hidekazu Takahashi1, Hisanori Hatano1, Daisuke
Takiuchi1, Koshi Mimori 2, Fumiaki Tanaka 2, Hiroaki Nagano 1, Mitsugu
Sekimoto 1, Yuichiro Doki 1, Masaki Mori 1, 2
(1 Department of Gastroenterological Surgery, Osaka University Graduate
School of Medicine, 2 Department of Molecular and Cellular Biology, Division of
Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu
University)

O-17

CD13 is a novel dormant cancer stem cell marker of liver and has a potency as anovel therapeutic target
Daisuke Takiuchi, Hideshi Ishii, Naotsugu Haraguchi, Hisanori Hatano,
Hidekazu Takahashi, Hiromitsu Hoshino, Norikatsu Miyoshi, Dyah Laksmi
Dewi, Hiroshi Wada, Shougo Kobayashi, Shigeru Marubashi, Hidetoshi Eguchi,
Yutaka Takeda, Masanori Tanemura, Hiroaki Nagano, Yuichiro Doki, Masaki
Mori(Department of Gastroenterological Surgery, Graduate school of medicine,
Osaka University)

O-18

Endometrial cancer side-population cells show prominent migration and have apotential to differentiate into the mesenchymal cell lineage
Kiyoko Kato1, Ayumi Kuboyama1, Tomoka Takao2, Norio Wake2(1Department of Obstetrics and Gynecology, Faculty of Medicine, Juntendo
University, 2Department of Obstetrics and Gynecology, School of Medicine,
Kyushu University)

O-19

Potential roles of NF-kappa B pathways in breast cancer-initiating cells
Kunihiko Hinohara1, Michiko Murohashi1, Takayuki Isagawa2, Shingo Tsuji2,
Kazuo Umezawa3, Hiroyuki Aburatani2, Noriko Gotoh1

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

(1 Division of Systems Biomedical Technology, Institute of Medical Science,
University of Tokyo, 2Genome Science Division, Research Center of Advanced
Science and Technology, University of Tokyo, 3Department of Applied Chemistry,
Faculty of Science and Technology, Keio University)

Coffee Break

15:00～15:20

Session 6：Cancer stem cell ( II )15:20～16:40

Chair Issay Kitabayashi(Division of Molecular Oncology, National Cancer Center Research Institute)

O-20

Regulation of stem cells in MOZ and other leukemias
Haruko Shima1, Yukiko Aikawa1, Kumiko Yamanaka1, Takuo Katsumoto1, Mika
Shino1, Akihiko Koseki2, Issay Kitabayashi1(1Division of Molecular Oncology, National Cancer Center Research Institute,
2Research Center for Allergy and Immunology, RIKEN)

O-21

Treatment with mTOR inhibitor, everolimus (RAD001) overcomes resistance toimatinib in Ph-leukemia quiescent or T315I-mutated cells
Yosuke Minami1, Yachiyo Kuwatsuka1, Miho Minami1, Ryohei Tanizaki1 andTomoki Naoe1(1Department of Hematology and Oncology, Nagoya University Graduate
School of Medicine)

O-22

Hematopoietic stem cells are primarily involved in pathogenesis of chronic
lymphocytic leukemia
Yoshikane Kikushige1, Toshihiro Miyamoto1, Fumihiko Ishikawa2, Koichi
Akashi1(1Medicine and Biosystemic Science, Kyushu University, 2Research Unit for
Human Disease Model, RIKEN Center for Allergy and Immunology)

O-23

Identification and targeting of multiple myeloma progenitor cells
Naoki Hosen, Satoshi Kishida, Yoshikazu Matsuoka, Haruo Sugiyama(Department of Functional Diagnostic Science, Osaka University Graduate
School of Medicine)

Coffee Break

16:40～17:00

Special Lecture 3
Chair Issay Kitabayashi

17:00～18:00

Regulation of normal and leukemic hematopoietic stem
cells by myeloid transcription factors
Daniel G. Tenen, M.D.
（Cancer Science Institute Singapore (CSI Singapore)

General Meeting

18:00～18:10

Poster Session

18:30～
8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

Saturday, May 15.

The Third Day

Session 7：Liver and muscle differentiation
Chair

8:30～9:30

Atsushi Miyajima(Institute of Molecular and Cellular Biosciences, The University of Tokyo)

O-24

FGF7 induces stem/progenitor cell response in the adult mouse liver
Hinako Takase, Tohru Itoh, Atsushi Miyajima（Institute of Molecular and Cellular Biosciences, The University of Tokyo）

O-25

Chd2 determines myogenic cell fateAkihito Harada1, Yasuyuki Ohkawa1,2(1Department of Epigenetics, SSP Stem Cell Unit, Faculty of Medicine, Kyushu
University, 2Institute for Advanced Study, Kyushu University)

O-26

BMP signalling permits population expansion by preventing premature
myogenic differentiation in muscle satellite cells
Yusuke Ono1, 2, Frederico Calbaheu2, Helge Amthor3, Takenobu Katagiri4 andPeter S. Zammit2(1Department of Molecular Therapy, National Institute of Neuroscience,
National Centre of Neurology and Psychiatry, Tokyo, Japan, 2Randall Divisionof Cell and Molecular Biophysics, King's College London, London, UK., 3UPMC
INSERM UMR S 974 / CNRS UMR 7215, Institut de Myologie, 105 bd del'Hôpital, Paris, France., 4Division of Pathophysiology, Research Centre for
Genomic Medicine, Saitama Medical University, Saitama, Japan)

Coffee Break

9:30～9:50

Session 8：Hematopoietic stem cell ( II )9:50～11:30

Chair

Mineo Kurokawa(Department of Hematology & Oncology, Graduate School of Medicine, University ofTokyo)

O-27

Molecular mechanism of HoxB4 mediated self-renewal
Mitsujiro Osawa1, Michael Kyba2(1 Department of Cellular and Molecular Medicine, Chiba University, Japan, 2Department of Pediatrics, University of Minnesota, MN, USA)

O-28

Co-repressor TIF1β is an essential regulator of hematopoietic stem cells
Satoru MIYAGI1,2, Issay KITABAYASHI3, Hitoshi ICHIKAWA4 and Atsushi
IWAMA1,2(1Department of Cellular and Molecular Medicine, Graduate School of
Medicine, Chiba university, 2.CREST, JST, 3.Molecular Oncology division,
National Cancer Institute, 4.Genetics division, National Cancer Institute)

O-29

Evi1 expression marks long-term repopulating hematopoietic stem cells.
Keisuke Kataoka1, 3, Tomohiko Sato1, 3, Akihide Yoshimi1, Susumu Goyama1,
Shunya Arai1, Yoichi Imai1, Katsuyoshi Kumagai2, Naoto Kubota2, Takashi
Kadowaki2, Mineo Kurokawa1(1Department of Hematology & Oncology, Graduate School of Medicine,
University of Tokyo, 2Department of Metabolic Disease, Graduate School of
Medicine, University of Tokyo, 3These authors contributed equally to thiswork.)

O-30

Strict regulation of mTOR signaling is essential for hematopoietic stem cellmaintenance in vivo
8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

Takayuki Hoshii1, Yuko Tadokoro1, Kazuhito Naka1, Takako Ooshio1, Teruyuki
Muraguchi1, Kimi Araki2, Ken-ichi Yamamura2, Atsushi Hirao1, 3(1Division of Molecular Genetics, Cancer Research Institute, Kanazawa
University, 2Laboratory of Developmental Genetics, IMEG, Kumamoto
University, 3CREST, JST)

O-31

Functional role of reactive oxygen species in lineage decision of myeloid
progenitor cells
Akihito Shinohara1, Yoichi Imai1, Masahiro Nakagawa1, Motoshi Ichikawa1,
Tsuyoshi Takahashi1, Mineo Kurokawa1,2(1Department of Hematology & Oncology, Graduate School of Medicine,
University of Tokyo, Tokyo, Japan, 2Department of Cell Therapy andTransplantation Medicine, University of Tokyo Hospital, Tokyo, Japan）

Closing Remarks

Next Director

Mineo Kurokawa

11:30～11:40

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

Poster Session
P-1

Thursday, May 13 & Friday, May 14.

18:30～G-CSF regulates skeletal muscle development and regeneration throughmyoblast-specific expression of the G-CSF receptor
Shinsuke Yuasa1, 2, Mie Hara1, Keiichi Fukuda1(1Department of Cardiology, Department of Internal Medicine, 2Center for Integrated Medical
Research, Keio University School of Medicine)

P-2

Generation of Induced Pluripotent Stem (iPS) Cells from Patients with Congenital Long
QT Syndrome
Tomohisa Seki1, Shinsuke Yuasa1, 2, Toru Egashira1, Keiichi Fukuda1(1Department of Cardiology, Department of Internal Medicine, 2Center for Integrated Medical
Research, Keio University School of Medicine)P-3

Identification, isolation and characterization of HCN4-positive cardiac pacemaking cells
derived from murine embryonic stem cells.
Yu Ikeuchi, Natsumi Shimizu, Shinichi Ito, Hiroshi Fujii, Kumi Morikawa, Yasuaki
Shirayoshi and Ichiro Hisatome.
（Division of Regenerative Medicine and Therapeutics, Graduate school of medical science,
Tottori University）

P-4

Global expression analysis of somatic stem cells in vitro
Satoko Yoshitake1, Takafusa Hikichi1, Atsushi Suzuki2, 3, Shinji Masui1, 3(1Research Institute, National Center for Global Health and Medicine, 2Medical Institute of
Bioregulation, Kyushu University, 3PRESTO, Japan Science and Technology Agency)P-5

A small molecule compound induces hepatic differentiation of human bonemarrow-derived mesenchymal stem cells by inhibition of Wnt/β-catenin signaling
Yoshiaki Matsumi, Noriko Matsumoto, An Afida Ashla, Yuta Tetsuka, Yuta Arakaki,
Yoshiko Hoshikawa, Goshi Shiota(Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University)

P-6

Establishment of mouse induced pluripotent stem cells expressing HNF3β in atetracycline-regulated fashion
Yuta Tetsuka1, Yoshiaki Matsumi1, Yoshikawa Hoshikawa1, Satsuki Miyazaki2, Jun-ichi
Miyazaki2, and Goshi Shiota1(1Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University,
2Division of Stem Cell Regulation Research, Graduate School of Medicine, Osaka University)

P-7

Impact of age on generation of IPSCs from mdx mouse fibroblast cells
Bo Wang, Makoto Segawa, Chika Harano, Yuko Miyagoe-Suzuki, Shin’ichi Takeda(Department of Molecular Therapy, National Institute of Neuroscience, National Center of
Neurology and Psychiatry, Tokyo, Japan)

P-8

The Effects of Arachidonic Acid and Docosahexaenoic Acid on Neural Stem/Progenitor Cells
Nobuyuki Sakayori1, Noriko Osumi1, 2, 3
(1Division of Developmental Neuroscience, Tohoku University Graduate School of Medicine,
2CREST, JST, 3Tohoku Neuroscience Global COE)

P-9

Altered brain microRNA biogenesis affects neural stem cell proliferation in mouse
hippocampal dentate gyrus
Yasuo Ouchi1, Yuko Simizu1, Mai Mizuno1, Takashi Iwamoto1(1Dept of Biomedical science, Chubu University)

P-10

The role of FABPs in postnatal hippocampal neurogenesis
Miho Matsumata1, 2, 3, Motoko Maekawa4, Yuji Owada5, Takeo Yoshikawa3, 4 and Noriko
Osumi1, 3, 6
(1Division of Developmental Neuroscience, Graduate School of Medicine, Tohoku University,
2Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical
University, 3CREST, JST, 4Brain Science of Institute, RIKEN, 5Department of Organ Anatomy,
Graduate School of Medicine, Yamaguchi University, 6GCOE, JSPS)

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

P-11

SIF is a secreted inducer of cell senescence expressed by aged CNS precursor cells
Toru Kondo1, 2 and Yuki Kujuro1(1Laboratory for Cell Lineage Modulation, RIKEN Center for Developmental Biology,
2Department of Stem Cell Biology, Ehime University Proteo-Medicine Research Center)P-12

Fbxw7α regulates the maintenance and differentiation of neural stem cells
Akinobu Matsumoto1, 2, Ichiro Onoyama1, 2, Takehiko Sunabori3, Yuki Tateishi1, 2,
Ryoichiro Kageyama2, 4, Hideyuki Okano3, Keiichi I. Nakayama1, 2(1Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu
University, 2CREST, 3Department of Physiology, Keio University School of Medicine,
4Institute for Virus Research, Kyoto University)

P-13

Molecular mechanism underlying cyclin D1 mediated inhibition of astrocyte
differentiation from neural stem/progenitor cells
Norihisa Bizen1, Toshihiro Inoue2, Takeshi Shimizu3, Tetsushi Kagawa1, Tetsuya Taga1(1Department of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and Dental
University, 2Department of Ophthalmology and Visual Science, Graduate School of Medical
Sciences, Kumamoto University, Laboratory for Vertebrate Axis Formation, RIKEN Center for
Developmental Biology)

P-14

Oxygen tension can control the DNA methylation status of GFAP promoter throughNotch signaling and allows propagation and maturation of neuronal progenitor
Tetsuji Mutoh1, Kinichi Nakashima1（ 1Graduate School of Biological
TECHNOLOGY.）

P-15

Sciences,

NARA INSTITUTEof

SIENCEandThe role of histone acetylation on cortical development
Berry Juliandi, Keita Tsujimura, Masahiko Abematsu, Jun Kohyama, Kinichi Nakashima(Laboratory of Molecular Neuroscience, Graduate School of Biological Science, Nara Instituteof Science and Technology)

P-16

ES specific transcription factors functions with the SWI/SNF chromatin remodelingfactor CHD2 in Embryonic stem cells
Jun Odawara1, 2, Akihito Harada1, Koichi Akashi2, Yasuyuki Ohkawa1(1Department of Epigenetics, Faculty of Medicine, Kyushu University, 2Medicine andBiosystemic Science, Kyushu University Graduate School of Medical Sciences)

P-17

Polycomb temporally regulates trophoblast development by promoting stem celldifferentiation
Mitsuhiro Endoh, Haruhiko Koseki(Developmental genetics, RIKEN RCAI)

P-18

Appropriate expression level of Eed is required for proper differentiation of ES cells
Hiroshi Koide1, Hiroki Ura1, Shinji Masui2, Hitoshi Niwa3, Tadayuki Akagi1, Takashi Yokota1(1Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University,
2Division of Molecular Biology and Cell Engineering, Department of Regenerative Medicine,Research Institte, International Medical Center of Japan, and 3Laboratory for Pluripotent
Cell Studies, RIKEN, Center for Developmental Biology)

P-19

Parameterising differentiation at the single cell level
Martin Jakt1, Satoko Moriwaki1, Shinichi Nishikawa1(1Stem Cell Biology Group, Riken Center for Developmental Biology, Kobe)

P-20

Functional analysis of Gli in mouse embryonic stem cells
Atsushi Ueda, Miwako Miura, Hiroki Ura, Tadayuki Akagi, Hiroshi Koide, Takashi Yokota(Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University)

P-21

Forced expression of telomerase catalytic subunit gene negatively regulates generation of
induced pluripotent stem cells
Akira Shimamoto, Yukihiro Sera, Kazuma Zensho, Yumiko Hino, Hidetoshi Tahara(Department of Cellular and Molecular Biology, Hiroshima University Graduate School of
Biomedical Sciences, Hiroshima, JAPAN)

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

P-22

Functional analysis of TERT and ATM in adult somatic cell reprogramming
Taisuke Kinoshita1, Go Nagamatsu1, Takeo Kosaka1, 2, Akitsu Hotta3, 4, James Ellis3-5,
Toshio Suda1(1Department of Cell Differentiation, The Sakaguchi Laboratory, 2Department of Urology, Schoolof Medicine, Keio University, 3Developmental and Stem Cell Biology Program and 4Ontario
Human iPS Cell Facility, SickKids, 5Department of Molecular Genetics, University of Toronto)

P-23

Metastable primordial germ cell-like state induced from mouse embryonic stem cells by
Akt activation
Tohru Kimura1, Noriko Yamano1, Takashi Shinohara3, Toru Nakano1, 2（1Graduate School of Frontier Biosciences, 2Medical School, Osaka University,
3Graduate School of Medicine, Kyoto University）

P-24

Mechanisms of primitive endoderm specification - from ES cells to preimplantation
mouse development
Julien Bouissac1, Naoko Yoshioka1, Shin-Ichi Nishikawa1(1Laboratory for Stem Cell Biology, RIKEN CDB )P-25

Screnning of surface antigens on iPS cells with SST-REX
Toshihiko Oki1, Toshio Kitamura1(1Division of Stem cell signaling, Stem cell Therapy Center, Institute of Medical Science,
University of Tokyo)

P-26

Heparan sulfate contributes to self-renewal and pluripotency in mouse embryonic stem cells
Kazumi Hirano1, Norihiko Sasaki1, Shoko Nishihara1(1Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka
University, Hachioji, Japan.)

P-27

Direct generation of induced pluripotent stem cells from human non-mobilized blood
Atsushi Kunisato1, Mariko Wakatsuki1, Haruna Shinba1, Toshio Ota2, Isao Ishida1, Kenji
Nagao1(1Frontier Laboratory, Kyowa Hakko Kirin Co., Ltd., 2Drug Discovery Research Laboratories,
Kyowa Hakko Kirin Co., Ltd.)

P-28

Purified mesenchymal stem cells: An efficient cell source for the iPS cells induction
Yoshimi Kawamura1, Kunimichi Niibe1, 2, Satoru Morikawa2, Yo Mabuchi1, Hideyuki Okano1,
Yumi Matsuzaki1(1Department of Physiology, 2Department of Dentistry and Oral Surgery, School of Medicine,
Keio University)

P-29

PROSPECTIVE CLONAL ISOLATION OF HUMAN MESENCHYMAL STEM CELLSELUCIDATES HETEROGENEITY WITHIN THE MULTI-POTENT STEM CELL
COMPARTMENT
Yo Mabuchi, Satoru Morikawa, Sadafumi Suzuki, Lawrence Lein, Kunimichi Niibe, Yasuo
Nagai, Takehiko Sunabori, Hideyuki Okano, Yumi Matsuzaki,(Physiology, Keio Univ School of Medicine, Tokyo, Japan)

P-30

Prospective isolation of mouse Sca-1+PDGFRα+ dental pulp stem cells (DPSCs)
existing in the tooth forming niche
Ryusuke Nakatsuka, Yasushi Uemura, Yoshikazu Matsuoka, Yutaka Sasaki, Yoshiaki Sonoda(Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical
Science, Kansai Medical University)

P-31

CD61/ integrin β3 ligation contributes to the thrombopoietin-mediatedmaintenance/expansion of mouse hematopoietic stem cells
Terumasa Umemoto1, Masayuki Yamato1, Yoshiko Shiratsuchi1, Kenji Tsukada1, 2, Mika Utsumi1,
Yohei Morita3, Masao Terasawa2, Takehiko Shibata2, Kohji Nishida4, Yoshiro Kobayashi2,
Brian Petrich5, Mark H. Ginsberg5, Hiromitsu Nakauchi3, Koji Eto3, and Teruo Okano1(1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University,
2Division of Molecular Medicine, Department of Biomolecular Science, Toho University, 3Stem Cell
Bank & Stem Cell Therapy, Center for Stem Cell Biology, The Institute of Medical Science, The
University of Tokyo, 4Department of Ophthalmology and Visual Science, Tohoku University
Graduate School of Medicine, and 5Department of Medicine, School of Medicine, University ofCalifornia)

8th Stem Cell Research Symposium

May 13 (Thu)-15(Sat), 2010

P-32

Transcriptional Mediator subunit MED1/TRAP220 in stromal cells is involved inhematopoietic stem/progenitor cell support through osteopontin expression
Ruri Ishino1, Akiko Sumitomo1, Norinaga Urahama1, Kana Inoue1, Kenji Yonezawa1,
Natsumi Hasegawa1, Chihiro Kaminaga1, Naohiko Seki2, Robert G. Roeder3, Mitsuhiro Ito1, 4(1Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate Schoolof Health Sciences, 2Department of Functional Genomics, Graduate School of Medicine, Chiba
University, 3Laboratory of Biochemistry and Molecular Biology, Rockefeller University, U.S.A.,
4Department of Family and Community Medicine, Kobe University Graduate School of
Medicine)

P-33

Role for Geminin in hematopoietic stem cell regulation
Shin’ichiro Yasunaga, Yoshinori Ohno, Motoaki Ohtsubo, Miyuki Tsumura, Yuka Kageyama,
Sayaka Mori, Yoshie Nakashima, Yoshihiro Takihara(Dept. Stem Cell Biol., RIRBM, Hiroshima Univ.)

P-34

An anti-apoptotic molecule Anamorsin plays significant roles in both intrinsic andextrinsic regulation of murine fetal liver hematopoiesis
Akira Tanimura, Hirokazu Tanaka, Yuri Saito, Hirohiko Shibayama, Itaru Matsumura, andYuzuru Kanakura(Department of Hematology and Oncology, Osaka University Graduate School of Medicine)

P-35

Cells that have hematopoietic activity in the placenta of mouse embryo reside in the side
population
Ahmed Ramadan1, 2, 3, Ikuo Nobuhisa1, 2, Shoutarou, Yamasaki1, Tetsuya Taga1, 2, 3
(1Dept. of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto
Univ., 2Dept. of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and Dental
Univ., 3Global COE, Kumamoto Univ. )P-36

c-Cbl regulates interaction of immature hematopoietic cells with the bone marrow
microenvironment by Rac GTPase-mediated cytoskeletal signals
Eisuke Uehara1, Takahiro Suzuki1, Hiroshi Okabe1, Masuzu Ueda1, Tadashi Nagai1,
Masashi Sanada2, Seishi Ogawa2, Keiya Ozawa1.
(1Division of Hematology, Department of Medicine, Jichi Medical University,
Genomics Project, Graduate School of Medicine, University of Tokyo.)P-37

2Cancer

Prospective purification of the human monocyte/macrophage progenitor
Takahiro Shima1, Toshihiro Miyamoto1, Yoshikane Kikushige1, Koichi Akashi1(1Medicine and Biosystemic Science, Kyushu University)

P-38

Microarray analysis of myeloid progenitors immortalized via retroviral transduction of Hes-1
Yasuyuki Miyake1, Mamiko Sakata-Yanaigmoto1, Takayasu Kato1, Hideharu Muto1, Noriko
Gotoh2 and Shigeru Chiba1(1Department of Clinical and Experimental Hematology, Graduate School of Comprehensive
Human Sciences, University of Tsukuba, 2Department of Systems Biomedical Technology,
Institute of Medical Science, University of Tokyo)

P-39

Standardized enumeration of human endothelial progenitor cells (EPCs) based on a flow
cytometric assay using the MACSQuant® Analyzer
Junji Tanaka, Kathrin Pütsch, Jürgen Schmitz(Miltenyi Biotec GmbH, Bergisch Gladbach, Germany)

P-40

Analysis of cancer stem cells in bone marrow metastasis by use of in vivo selection system
Tsuyoshi Shirakawa, Keita Uchino, Hitoshi Kusaba, Eishi Baba and Koichi Akashi(Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Science)

P-41

C6 glioma main population maintains side population
Kouichi Tabu1,2, Yasuhiro Kokubu1, Norihisa Bizen1, Ikuo Nobuhisa1, Tetsushi Kagawa1,
Tetsuya Taga1(1Department of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and Dental
University, 2JSPS Research Fellow)

8th Stem Cell Research Symposium