京都大学教育研究振興財団助成事業
成 果 報 告 書
平成27年9月16日
公益財団法人京都大学教育研究振興財団会 長 辻
井 昭 雄 様所属部局・研究科

京都大学医学研究科

職 名・学 年

博士課程４年

氏

Saeed Idrees

名助 成 の 種 類

平成２７年度 ・ 若手研究者在外研究支援 ・ 国際研究集会発表助成

研 究 集 会 名

第５５回 国際歯科学研究学会オーストラリア・ニュージーランド総会・学術大会

発 表 題 目

ウサギ顎関節骨軟骨欠損に対する滑液中未分化間葉系細胞による骨軟骨組織再建に関する研究

開 催 場 所ニュージーランド オタゴ ダニーデン オタゴ大学

渡 航 期 間平成27年8月24日

成 果 の 概 要～

平成27年8月29日

タイトルは「成果の概要／報告者名」として、A４版２０００字程度・和文で作成し、添付して下さい。 「成果の概要」以外に添付する資料 ■ 無□ 有()
交付を受けた助成金額250,000円

使 用 し た 助 成 金 額

250,000円

返納すべき助成金額0円
往復航空券代 175,220円
宿泊費会 計 報 告8579円

参加登録費 50,505円
助 成 金 の 使 途 内 訳

査証手数費 20,800円（今回の助成に対する感想、今後の助成に望むこと等お書き下さい。助成事業の参考にさせていただきます。）

当財団の助成についてPost-Conference Report
Executive Summary
Attendee Name

Saeed Idrees

Attendee Title

Doctoral Student

Department

Department of Oral and Maxillofacial Surgery
Graduate School of Medicine, Kyoto University

Conference Summary

55TH ANNUAL SCIENTIFIC MEETING OF THE IADR ANZDIVISION DUNEDIN PUBLIC ART GALLERY THE OCTAGON, DUNEDIN, NZ 2326 AUGUST, 2015
The scientific theme of IADR ANZ 2015 was: Translational dentistry from thelaboratory to the clinic.
This is a timely theme and one that is increasingly more important in clinicalpractice, research funding, and government policy.
The meeting was a great success with over 180 delegates attending from morethan a dozen countries.

Conference URL

http://www.otago.ac.nz/iadranz-2015/index.html
http://www.otago.ac.nz/sjwri/otago119420.pdf

Goals Met1

Presented my research : Rabbit TMJ osteochondral defect regeneration using TMJ synovial fluid MSCs
in the last Session'15* Biomechanics and Tissue Engineering 26th August 2015 Abstract ID:2330193

2

Met and discussed about research (regenerative medicine, Nano technology for dental materials) anddental education with people from all over the world.

Business Relationships
Name

Professor Benjamin Wu

Contact Details

bwu@dentistry.ucla.edu

Professor and Chair of the Division of Advanced Prosthodontics, and the Director of the Weintraub
Center for Reconstructive Biotechnology at the UCLA School of Dentistry.
Name

Mohammad Alansary

Contact Details

ansary2006uk@msn.com

PhD candidate, Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago
Research field : regenerative medicine , tooth regeneration
NameGemma Cotton

Contact Details

gcotton1@hotmail.com

PhD candidate, Department of Chemistry and Faculty of Dentistry, University of Otago
Research field : nanocomposite ,dental materials

1

Abstract Published online:
Rabbit TMJ osteochondral defect regeneration using TMJ synovial
fluid MSCs
Saeed Idrees1-2, Shigeki Yamanaka 1, Kazumasa Nakao1, Hiroyuki
Takafuji1, Kazuma Fujimura 1-3, Kazuhisa Bessho1
1

Department of Oral and Maxillofacial Surgery Graduate School of Medicine, Kyoto University, 54
Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
2

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tishreen
University, Latakia, Syria.
3
Department of Oral and Maxillofacial Surgery, Hyogo Prefectural Amagasaki General
Medical Center, Higashi-Naniwa 2-17-77, Amagasaki, Hyogo 660-0892, Japan.
Background: The most common joint pathology affecting the temporomandibular joint(TMJ) is the degenerative joint disease, also known as osteoarthrosis or osteoarthritis.
Among individuals with TMJ disorders, 11% have symptoms of TMJ-osteoarthritis(TMJ-OA). Once the breakdown in the joint starts, TMJ-OA can be crippling, leading toa variety of morphological and functional deformities.
Objective: Adequate amount of MSCs can be obtained from SF of TMJ with relative easewithout injuring healthy tissue (TMJ arthrocentesis is a routine procedure in oral andmaxillofacial departments for temporomandibular joint disorders-TMD- patients). The
aim of this study is to regenerate the cartilage with osteochondral defects on the articularsurface of the TMJ using mesenchymal stem cells (MSCs) of TMJ synovial fluid (SF).
Method: In this study MSCs concentration in the TMJ SF was increased by inducing
osteoarthritis by mechanical overloading for 4 weeks in Japanese white rabbits TMJs.
Rabbits were chosen because their size is suitable for a good anatomical observation andmanipulation.
Cells were aspirated from the TMJ (in a procedure stimulating TMJ Arthrocentesis we dofor TMD patients) then cultured in cell culture dishes. Subsequently, 200,000 cells wereplaced in 15-ml polypropylene tube and centrifuged at 450 g for 10 min thenTMJSFMSCs pellets were cultured in a chondrogenesis media to differentiate into
chondrocytes for 21 days. Differentiated chondrocytes were transplanted to the defects of
the TMJ articular surface in six rabbits.
Results: Histological examinations showed significant regeneration of the cartilage
compared to control group. Further, our data indicate that TMJSFMSCs may be similar tobone marrow derived MSCs and express similar cell surface markers.
Conclusion: TMJSFMSCs may be a promising candidate cell type for cell-based
strategies for articular cartilage repair in the future.

2