protease TMPRSS2 activates hepatitis C virus

2. Department of Virology II

infection. Hepatology. 61(2):437-46, 2015

1.

Arita M, Kilpatrick DR, Nakamura T, Burns CC,

Wakita T, Takeda N, Li TC. Characterization ofPallansch MA, Shimizu H. Development of anhumanefficient entire-capsid-coding-region amplification

recombinant

method for direct detection of poliovirus from stool

207:38-44, 2014

9.

Virol

Methods.

Fujii Y, Nakagomi T, Nishimura N, Noguchi A,

cholesterol

predominanceon

poliovirus-induced

Microbiology

andmembrane

Immunology,

58:

K,

Nakagomi
in

Japan

O:
of

Spreadnovel

andG1P[8]

double-reassortant rotavirus strains possessing a239-256, 2014

DS-1-like genotype constellation typical of G2P[4]Choi JE, Hur W, Kim JH, Li TZ, Lee EB, Lee SW,

strains. Infect Genet Evol. 28:426-33, 2014

Yoon

SK.

10. Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa

infectioninM, Yamamoto S, Motomura T, Okamoto T, Okuzaki

differentiated hepatocyte-like cells from adipose

D, Yamamoto M, Saito I, Wakita T, Koike K,

tissue-derived mesenchymal stem cells. PLoS One.

Matsuura13;9(5):e91958. 2014

apolipoproteins are crucial to the formation of

Daito T, Watashi K, Sluder A, Ohashi H, Nakajima S,

infectious hepatitis C virus particles. PLoS Pathog.

Borroto-Esoda K, Fujita T, Wakita T. Cyclophilin

11;10(12):e1004534. 2014

W,

Shin

EC,

Wakitamodulates

T,

HCV

Y.

Amphipathic

α-helicesinof

11. Hiramoto H, Dansako H, Takeda M, Satoh S, Wakita

RNA-dependent protein kinase to restore expression

T, Ikeda M, Kato N. Annexin A1 negativelyof IFN-stimulated genes in HCV-infected cells.

regulates viral RNA replication of hepatitis C virus.

Gastroenterology 147(2): 463-472, 2014.

Acta Med Okayama. 69(2):71-8. 2015

reducephosphorylation

Dansako H, Hiramoto H, Ikeda M, Wakita T, Kato N.

12. Ishibashi M, Morita N, Nomura-Kawaguchi C,

Rab18 is required for viral assembly of hepatitis C

Shimizu Y, Wakita T, Esumi M. CLEC4M-positive

virus through trafficking of the core protein to lipid

and CD81-negative Huh7 cells are not susceptible todroplets. Virology. 462-463:166-74, 2014

JFH-1 HCVcc infection but mediate transinfection.

Dennis FE, Fujii Y, Haga K, Damanka S, Lartey B,

Arch Virol.159(11):2949-55. 2014

Agbemabiese CA, Ohta N, Armah GE, Katayama K:
Identification

7.

J

by

Katayama

inhibitors

6.

baculoviruses.

generatedoxysterol-binding protein accumulate unesterified

MicroRNA-27a5.

particles

Miura S, Ito H, Doan YH, Takahashi T, Ozaki T,

Kang

4.

bocavirus-likeArita M. Phosphatidylinositol-4 kinase III beta andstructure.

3.

Fang L, Wang Z, Song S, Kataoka M, Ke C, Suzuki T,

Bukbuk D, Oderinde SB, Oberste MS, Kew OM,

extracts. J Clin Microbiol 53: 73-78, 2015

2.

8.

of

G8P[6]

MB, Aoto K, Wu YX, Sato E, Kobayashi Y, Wakita

Human-Bovine Reassortant Rotavirus Strain by

T, Miura N. Pol I-transcribed hepatitis C virus

Nextgenome RNA replicates, produces an infectious virus

Generation

Novel

Ghanaian

13. Islam MJ, Hikosaka K, Noritake H, Uddin MK, Amin

Sequencing.

PLoS

One

9(6):e100699. 2014

and leads to severe hepatic steatosis in transgenic

Esumi M, Ishibashi M, Yamaguchi H, Nakajima S,

mice. Biomed Res. 36(3):159-67. 2015

Tai Y, Kikuta S, Sugitani M, Takayama T, Tahara M,

14. Iwamoto M, Watashi K, Tsukuda S, Aly HH,

Takeda M, Wakita T. Transmembrane serine

Fukasawa M, Fujimoto A, Suzuki R, Aizaki H, Ito T,Koiwai O, Kusuhara H, Wakita T. Evaluation ands and Evolution, 27, 277-293, 2014

identification of hepatitis B virus entry inhibitors

21. Kondo Y, Ninomiya M, Kimura O, Machida K,

using HepG2 cells overexpressing a membrane

Funayama R, Nagashima T, Kobayashi K, Kakazu E,

transporter NTCP. Biochem Biophys Res Commun

Kato T, Nakayama K, Lai MM, Shimosegawa T.

443(3): 808-813, 2014.

HCV infection enhances Th17 commitment, which

15. Jiang X, Kanda T, Wu S, Nakamoto S, Wakita T,
Shirasawa H, Yokosuka O. Hepatitis C virus
nonstructuralprotein5A

could affect the pathogenesis of autoimmune
diseases. PloS One 9(6), e98521, 2014.

inhibits

22. Li TC, Yang T, Yoshizaki S, Ami Y, Suzaki Y, Ishii

thapsigargin-induced apoptosis. PLoS One. ; 9(11):K, Haga K, Nakamura T, Ochiai S, Wakita T, Johnee113499. 2014

R. Construction and characterization of an infectious

16. Jiang X, Kanda T, Wu S, Nakamoto S, Saito K,
Shirasawa H, Kiyohara T, Ishii K, Wakita T,

cDNA clone of rat hepatitis E virus. Journal of
General Virology 96:1320-7, 2015

Okamoto H, Yokosuka O. Suppression of La Antigen

23. Li TC, Yang T, Shiota T, Yoshizaki S, Yoshida H,

Exerts Potential Antiviral Effects against Hepatitis A

Saito M, Imagawa T, Malbas F, Lupisan S, Oshitani

Virus. PLOS One, 9, e101993, 2014

H, Wakita T, Ishii K. Molecular detection of

17. Katayama K, Murakami K, Sharp TM, Guix S, Oka T,

hepatitis E virus in rivers in the Philippines.

Takai-Todaka R, Nakanishi A, Crawford SE, Atmar

American Journal of Tropical Medicine and Hygine,

RL, Estes MK.

90: 764-766, 2014

Plasmid-based human norovirus

reverse genetics system produces reporter-tagged

24. Li TC, Fukumoto H, Hasegawa H, Wakita T, Saeki

progeny virus containing infectious genomic RNA.

H, Suzuki T, Katano H. Seroprevalence ofProc Natl Acad Sci U S A. 111(38):E4043-52, 2014

trichodysplasia spinulosa-associated polyomavirus

18. Kim S, Date T, Yokokawa H, Kono T, Aizaki H,

in Japan. J Clinic Virol. 65:76-82, 2015

Maurel P, Gondeau C, Wakita T. Development of

25. Li TC, Iwasaki K, Katano H, Kataoka M, Nagata N,

hepatitis C virus genotype 3a cell culture system.

Kobayashi K, Mizutani T, Takeda M, Wakita T,

Hepatology. 60(6):1838-50. 2014

Suzuki T. Characterization of Self-assembled

19. Komoto S, Pongsuwanna Y, Ide T, Wakuda M,
Guntapong R, Dennis FE, Haga K, Fujii Y,

Virus-like Particles of Merkel Cell Polyomavirus.
PLOS ONE, 10(2):e0115646,2015

Katayama K, Taniguti K. Whole genomic analysis of

26. Li TC, Yang T, Ami Y, Suzaki Y, Shirakura M,

porcine G10P[5] rotavirus strain P343 provides

Kishida N, Asanuma H, Takeda N, and Wakita T.

evidence

interspecies

Full Genome of Ferret Hepatitis E Virus fromtransmission. Veterinary Microbiology 174, 577-583,

Laboratory Ferrets. Emerg Infect Dis. 20(4):709-12,

2014.

2014

for

bovine-to-porcine

20. Komoto S, Wandera Apondi E, Shah M, Odoyo E,

27. Li TC, Yonemitsu K, Terada Y, Takeda N, Wakita T

Nyangao J, Tomita M, Wakuda M, Maeno Y, Shirat

and Maeda K. Ferret hepatitis E virus infection ino H, Tsuji T, Ichinose Y, Taniguchi K. Whole geno

Japan. JJID 68(1).60-62, 2015

mic analysis of human G12P[6] and G12P[8] rotavi

28. Li Y, Masaki T, Shimakami T, Lemon SM. hnRNP L

rus strains that have emerged in Kenya: Identifica

and NF90 interact with hepatitis C virus 5'-terminaltion of porcine-like NSP4 genes. Infection, Genetic

untranslated RNA and promote efficient replication.

J Virol 88(13): 7199-7209, 2014.

entry

29. Lin CM, Gao X, Oka T, Vlasova AN, Esseili MA,ofhepatitisCvirus.

J

Gen

Virol

95(Pt12):2658-2667, 2014.

Wang Q, Saif LJ. Antigenic relationships among

35. McGivern DR, Masaki T, Williford S, Ingravallo P,

porcine epidemic diarrhea virus and transmissible

Feng Z, Lahser F, Asante-Appiah E, Neddermann P,

gastroenteritis virus strains. J Virol. 89 (6): 3332-42,

De Francesco R, Howe AY, Lemon SM. Kinetic

2015

analyses reveal potent and early blockade of

30. Liu X, Saito M, Sayama Y, Suzuki E, Malbas FF,
Galang HO, Furuse Y, Saito M, Li TC, Suzuki A,
Oshitani

H.

Seroprevalenceandmolecular

of

E

virus

characteristics

hepatitisinhepatitis C virus assembly by NS5A inhibitors.
Gastroenterology 147(2): 453-462, 2014.

36. Munakata T, Inada M, Tokunaga Y, Wakita T,
Kohara M, Nomoto A. Suppression of hepatitis C

household-raised pig population in Philippines.

virus

replicationbycyclin-dependent

BMC Vet Res. 11(1):11, 2015

inhibitors. Antiviral Res. 108:79-87, 2014

kinase31. Masaki T, Arend KC, Li Y, Yamane D, McGivern

37. Naeem A, Hosomi T, Nishimura Y, Alam MM, Oka

DR, Kato T, Wakita T, Moorman NJ, Lemon SM.

T, Zaidi SS, Shimizu H. Genetic diversity of

miR-122 stimulates hepatitis C virus RNA synthesis

circulatingby altering the balance of viral RNAs engaged in

Afghanistan. J Gen Virol 95: 1945-1957, 2014

replication versus translation. Cell Host Microbe

38. Nakai M, Seya T, Matsumoto M, Shimotohno K,

17(2): 217-228, 2015.

Saffold

virusesinPakistanand

Sakamoto N, Aly HH. The J6JFH1 strain of

32. Masaki T, Matsunaga S, Takahashi H, Nakashima K,

hepatitis C virus infects human B-cells with low

Kimura Y, Ito M, Matsuda M, Murayama A, Kato T,

replication efficacy. Viral Immunol 27(6): 285-94,

Hirano H, Endo Y, Lemon SM, Wakita T, Sawasaki

2014.

T, Suzuki T. Involvement of hepatitis C virus NS5A

39. Ogura N, Watashi K, Noguchi T, Wakita T.

hyperphosphorylation mediated by casein kinase

Formation of covalently closed circular DNA in

I-α in infectious virus production. J Virol 88(13):Hep38.7-Tet cells, a tetracycline inducible hepatitis

7541-7555, 2014.

B virus expression cell line. Biochem Biophys Res

33. Masuda T, Nagai M, Yamasato H, Tsuchiaka S,

Commun 452(3): 315-321, 2014.

Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa

40. Oka T, Saif LJ, Marthaler D, Esseili MA, Meulia T,

Y, Omatsu T, Furuya T, Koyama S, Shirai J,

Lin CM, Vlasova AN, Jung K, Zhang Y, Wang Q.

Taniguchi K, Fujii Y, Todaka R, Katayama K,

Cell culture isolation and sequence analysis of

Mizutani T: Identification of novel bovine group A

genetically diverse US porcine epidemic diarrhea

rotavirus G15P[14] strain from epizootic diarrhea

virus strains including a novel strain with a largeof adult cows by de novo sequencing using adeletion in the spike gene. Vet Microbiol. 173 (3-4):

next-generation

258-69, 2014

sequencer.

Vet

Microbiol

171(1-2):66-73, 2014

34. Matsuda M, Suzuki R, Kataoka C, Watashi K,

41. Oka T, Saif LJ, Yang Z, Scheuer KA, Stoltzfus GT,
Wang Q. First Complete Genome Sequences of

Aizaki H, Kato N, Matsuura Y, Suzuki T, Wakita T.

Genogroup

VI

PorcineAlternative endocytosis pathway for productive

Announc. 2 (2).2014

Sapoviruses

Genome42. Oka T, Takagi H, Tohya Y. Development of a novel

Masaki T, Fannin EE, Guerra B, Shirasaki T,

single step reverse genetics system for feline

Shimakami T, Kaneko S, Lanford RE, Lemon SM,

calicivirus. J Virol Methods. 207:178-81, 2014

Sethupathy P. Small tRNA-derived RNAs are

43. Oka T, Wang Q, Katayama K, Saif LJ.

increased and more abundant than microRNAs in

Comprehensive review of human sapoviruses. Clin
Microbiol Rev. 28 (1): 32-53, 2015

chronic hepatitis B and C. Sci Rep 5: 7675, 2015.

50. Shibata S, Sekizuka T, Kodaira A, Kuroda M, Haga

44. Okitsu S, Khamrin P, Thongprachum A, Kalesaran

K, Doan YH, Takai-Todaka R, Katayama K, Wakita

AF, Takanashi S, Shimizu H, Maneekarn N,

T, Oka T, Hirata H. Complete Genome Sequence of a

Mizuguchi M, Hayakawa S, Ushijima H. Molecular

Novel GV.2 Sapovirus Strain, NGY-1, Detectedcharacterization and sequence analysis of the 2B

fromregion of Aichivirus C strains in Japan and Thailand.

Outbreak. Genome Announc. 3(1). pii: e01553-14,

Infect Genet Evol 26: 89-94, 2014

2015

a

Suspected

Foodborne

Gastroenteritis

45. Okitsu S, Khamrin P, Thongprachum A, Nishimura S,

51. Shimizu H, Nakashima K. Surveillance of hand, foot,Kalesaran AF, Takanashi S, Shimizu H, Hayakawa S,

and mouth disease for a vaccine. Lancet Infect. Dis.

Mizuguchi M, Ushijima H. Detection and molecular

14(4), 262-3, 2014

characterization of human cosavirus in a pediatric

52. Shiokawa M, Fukuhara T, Ono C, Yamamoto S,

patient with acute gastroenteritis, Japan. Infect Genet

Okamoto T, Watanabe N, Wakita T, Matsuura Y.

Evol 28: 125-129, 2014

Novel permissive cell lines for complete propagation

46. Saito K, Shirasago Y, Suzuki T, Aizaki H, Hanada K,of hepatitis C virus. J Virol. 88(10):5578-94, 2014

Wakita T, Nishijima M, Fukasawa M. Targeting

53. Shiota T, Li TC, Yoshizaki S, Kato T, Wakita T,

cellular squalene synthase, an enzyme essential for

Ishii K. Establishment of hepatitis E virus

cholesterol biosynthesis, is a potential antiviral

infection-permissive and -non-permissive humanstrategy

hepatomaagainsthepatitisCvirus.

J

Virol.

89(4):2220-32, 2015

PLC/PRF/5

subclones.

Microbiol

Immunol 59(2):89-94, 2015

47. Sato A, Kameyama K, Nagai M, Tateishi K, Ohmori

54. Shirasago Y, Sekizuka T, Saito K, Suzuki T, Wakita

K, Todaka R, Katayama K, Mizutani T, Yamakawa

T, Hanada K, Kuroda M, Abe R, Fukasawa M.

M, Shirai J. Complete Genome Sequence of Bovine

Isolation

Viral Diarrhea Virus 2 Japanese Reference andHuh.7.5.1-derived cell clone highly permissive to

Vaccine Strain KZ-91CP. Genome Announc. 3(1).

hepatitis C virus. Jpn J Infect Dis. 68(2):81-8, 2015

pii: e01573-14, 2015

andcharacterizationofan

55. Shirato H, Someya Y, Ochiai M, Horiuchi Y,

48. Sato S, Li K, Kameyama T, Hayashi T, Ishida Y,

Takahashi M, Takeda N, Wakabayashi K, Ouchi Y,

Murakami S, Watanabe T, Iijima S, Sakurai Y,

Ota Y, Tano Y, Abe S, Yamazaki S, Wakita T, sIPV

Watashi K, Tsutsumi S, Sato Y, Akita H, Wakita T,

Evaluation Group of NIID Virology II. A national

Rice CM, Harashima H, Kohara M, Tanaka Y,

reference for inactivated polio vaccine derived from

Takaoka A. The RNA sensor RIG-I dually functions

Sabin strains in Japan. Vaccine 32: 5163-5169, 2014as an innate sensor and direct antiviral factor for

56. Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa

hepatitis B virus. Immunity 42(1):123-32, 2015

N, Murakami Y, Chu PS, Usui S, Ishibashi Y,

49. Selitsky SR, Baran-Gale J, Honda M, Yamane D,

Wakayama Y, Taniki N, Murata H, Saito Y,

Fukasawa M, Saito K, Yamagishi Y, Wakita T,

Temporal Fluctuation and Molecular Epidemiology.

Takaku H, Hibi T, Saito H, Kanai T. ProminentAppl Environ Microbiol. 80(15): 4683-4691, 2014steatosis with hypermetabolism of the cell line

63. Watashi K, Sluder A, Daito T, Matsunaga S, Ryo A,

permissive for years of infection with hepatitis C

Nagamori S, Iwamoto M, Nakajima S, Tsukuda S,

virus. PLoS One. 9(4):e94460, 2014

Borroto-Esoda K, Sugiyama M, Tanaka Y, Kanai Y,

57. Sugiyama N, Murayama A, Suzuki R, Watanabe N,

Kusuhara H, Mizokami M, Wakita T. Cyclosporin

Shiina M, Liang TJ, Wakita T, Kato T. Single strain

A and its analogs inhibit hepatitis B virus entry into

isolation method for cell culture-adapted hepatitis C

cultured hepatocytes through targeting a membrane

virus by end-point dilution and infection. PLoS One

transporter, sodium taurocholate cotransporting

9(5): e98168, 2014.

polypeptide (NTCP). Hepatology 59(5): 1726-1737,

58. Sung PS, Murayama A, Kang W, Kim MS, Yoon SK,

2014.

Fukasawa M, Kondoh M, Kim JS, Kim H, Kato T,

64. Watashi K, Urban S, Li W, Wakita T. NTCP and

Shin EC. Hepatitis C virus entry is impaired bybeyond: opening the door to unveil hepatitis B virus

claudin-1

entry. Int J Mol Sci 15(2): 2892-2905, 2014.

downregulationindiacylglycerol

acyltransferase-1-deficient cells. J Virol. 88(16):AG, Arita M, Boesch AW, Ackerman ME, van der9233-9244, 2014.

59. Tang J, Yoshida H, Ding Z, Tao Z, Zhang J, Tian B,
Zhang

L.

Molecular

Epidemiology

andRecombination of Human Enteroviruses from AFP
surveillance in Yunnan, China from 2006 to 2010.

60. Tanida I, Shirasago Y, Suzuki R, Abe R, Wakita T,
K,

Fukasawa

M.

Avoort, MH. Oberste S, Pallansch MA, Burton AH,
Jaffar MA, and Sutter RW. Intestinal immunity is adeterminant of clearance of poliovirus after oralvaccination. Journal of Infectious Diseases, 209:
1628-1634, 2014

Scientific reports, 4: 6058, 2014

Hanada

65. Wright PF, Wieland-Alter W, Ilyushina NA, Hoen

Caffeicacid,acoffee-related organic acid, inhibits the propagationof hepatitis C virus. Jpn J Infect Dis. 68:268-275,
2015.

61. Tsukuda S, Watashi K, Iwamoto M, Suzuki R, Aizaki
H, Okada M, Sugiyama M, Kojima S, Tanaka Y,
Mizokami M, Li J, Tong S, Wakita T. Dysregulationof retinoic acid receptor diminishes hepatocyte
permissiveness to hepatitis B virus infection throughmodulation of sodium taurocholate cotransporting
polypeptide (NTCP) expression. J Biol Chem.
290(9):5673-84, 2015

62. Wang H, Tao Z, Li Y, Lin X, Yoshida H, Song L,
Zhang Y, Wang S, Cui N, Xu W, Song Y, Xu A.
Environmental Surveillance of Human Enterovirusesin Shandong Province, China, 2008-2012: Serotypes,

66. Xeuatvongsa

A,

KomadaVongphrachanh
Phounphenghak

P,
K,

K,

Kitamura

Pathammavong
SisoukT,

Phonekeo

T,C,
D,

Sengkeopaseuth B, Som-Oulay V, Ishii K, Wakita T,
Sugiyama M, Hachiya M. Chronic Hepatitis B
Prevalence among Children and Mothers: Resultsfrom a Nationwide, Population-Based Survey in Lao
People’s Democratic Republic. PLoS One, 9, e88829,
2014