Jie Lin
Ph.D., in Plant Metabolic Bioengineering
E-mail: lj0215@yorku.ca
EDUCATION & WORK EXPERIENCE
2020−presentPh.D. program in Biology
York University, Toronto, Canada

2017 −2020

Master’s Degree of Ornamental Horticulture
Southwest University, Chongqing, China

2013 −2017

Bachelor of Horticulture (Ornamental Horticulture)
Hunan Agricultural University, Changsha, China

RESEARCH OUTLINES(1) Identifying the regulatory networks of glyceollin transcription factors in soybean (Glycine max
L. Merr), using RNAseq data to search for the potential positive regulators base on the previous
research in our lab.
(2) Studying the protein-protein interactions using yeast two-hybrid, Bimolecular fluorescencecomplementary assay. And the protein-DNA interactions using yeast-one hybrid, protoplasttransient assay.
(3) Investigating the transcription factors’ function by overexpressing and RNA interference
silencing them in soybean hairy root system individually.
TECHNICAL EXPERTISE
 Gene cloning and Agrobacteria transformation.
 Use and basic maintain of various analytical instruments, such as UPLC and RT-qPCR.
 Data analysis, such as ANOVA and T-test.
LEADERSHIP &COLLABORATIONS Taking a leadership role in the ‘Phytoalexin gene regulatory networks’ project.
 Training students, developing their lab skills of molecular biology, and providing support inexperiment and data analysis.
 Collaborating and liaising with partners, including Agriculture and Agri-food Canada.

PUBLICATIONS
Lin, J., Wi, D., Ly, M., Jahan, M. A., Pullano, S., Martirosyan, I., Kovinich, N. Soybean Hairy
Root Transformation for the Analysis of Gene Function. JoVE (195), e65485,
doi:10.3791/65485 (2023).
Lin, J., Monsalvo, I., Ly, M., Jahan, M. A., Wi, D., Martirosyan, I., & Kovinich, N. (2023). RNASeq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean
Transcription Factors GmMYB29A2 and GmNAC42-1. Plants, 12(3), 545.
Lin, J., Liu, D., Wang, X., Ahmed, S., Li, M., Kovinich, N., & Sui, S. (2021). Transgene
CpNAC68 from wintersweet (Chimonanthus praecox) improves Arabidopsis survival ofmultiple abiotic stresses. Plants, 10(7), 1403.
Yousefi-Taemeh, M., Lin, J., Ifa, D. R., Parrott, W., & Kovinich, N. (2021). Metabolomics
Differences of Glycine max QTLs Resistant to Soybean Looper. Metabolites, 11(10), 710.
Wang, X., Liu, D., Lin, J., Zhu, T., Liu, N., Yang, X., ... & Sui, S. (2021). Carotenoid cleavage
dioxygenase genes of Chimonanthus praecox, CpCCD7 and CpCCD8, regulate shootbranching in Arabidopsis. International Journal of Molecular Sciences, 22(16), 8750.
Papers currently undergoing peer-review
Lin, J., Monsalvo, I., Ly, M., Wi, D., Jahan, MA., Martirosyan, I., Jahan, I., and Kovinich, N. ABA-regulated JAZ1
Proteins Bind NAC42 Transcription Factors to Suppress the Activation of Phytoalexin Biosynthesis in Plants
Submitted to Plant physiology on February 17, 2023.

CONFERENCE PRESENTATIONS
Lin, J., Monsalvo, I., Ly, M., Wi, D., Jahan, MA., Martirosyan, I., Jahan, I., and Kovinich, N. JAZ1 proteins bind
NAC42 transcription factors to suppress the biosynthesis of diverse phytoalexins in plants. Canadian Society pf
Plant Biologist, Quebec City, Quebec, Canada, June 18–21, 2023
Lin, J., Monsalvo, I., Ly, M., Wi, D., and Kovinich, N. Identifying the regulatory networks of glyceollintranscription factors in soybean (Glycine max L. Merr). Association of Graduate Students in the Biological

Sciences Symposium, Toronto, ON, Canada, May 11, 2023
Lin, J., Monsalvo, I., Ly, M., and Kovinich, N. Identifying missing glyceollin transcription factors in soybean(Glycine max L. Merr). Phytochemistry Socirty of North America, Blacksburg, VA, USA, July 24-28, 2022.
(Online presentation due to US Visa issue)