Neutral OptiMS Capillary Cartridge
The Neutral OptiMS capillary cartridge is designed for use
with the CESI 8000 Plus High Performance Separation
Module for CESI-MS applications. Its hydrophilic neutral
surface virtually eliminates electroosmotic flow, increasing
the size of the separation window, helping resolve
closely migrating protein isoforms. In addition, thissurface minimizes interaction between analytes and thecapillary wall, increasing overall sensitivity. The enhanced
resolution combined with the superior sensitivity andreduced ion suppression of CESI-MS, help you see whatyou otherwise would be missing.

CESI Sprayer Schematic
As mass spectrometry researchers sought to expand theirrange of detection and increase sensitivity, ultra low-flow
separation was integrated with mass spectrometry using
proprietary CESI-MS technology.
The neutral capillary version of the sprayer cartridge expands
the separation window and allows for working with analytes
that may interact with the bare fused-silica capillary model.

SCIEX’s Neutral OptiMS technology delivers robust
separation and mass spectrometry analysis of:•Intact proteins, and protein mixtures•Complex peptide digests•

Cationic and anionic metabolites in biofluids

Separation
CapillaryPorousCapillary
Segment

ESI Needle

ConductiveLiquid

The Neutral Capillary interior has a binary layer coating:Hydrophobic Coating• Protects siloxanes from hydrolysis for increased longevity

Hydrophilic polyacrylamide surface•V irtually eliminates electroosmotic flow, increasing the separation window• Inhibits protein interactions

with the capillary wall increasing sensitivity• Significantly broaden the pH range from pH 2 to 9

Neutral OptiMS CESI-MS data fromrepeat analysis of a three protein
mixture sample
Sample consists of Cytochrome
C, Lysozyme, and Ribonuclease B,
shown as major peaks in the Total Ion
Chromatograms eluting from left toright, respectively. A total of 176 runs ofthe same three-protein mixture sample
was performed over 6 days on a single
Neutral OptiMS Capillary Cartridge.
Protein concentration used was 0.1 mg/mL for each protein, dissolved in 50 mM
Ammonium Acetate, pH 3.0, with < 50
nL injected per analysis. The m/z range
monitored was 400 to 3000.
Number of runs established on each cartridge issample, BGE and condition dependent.

Additional CESI components & reagents:•A 98089 – CESI 8000 and CESI 8000 Plus High Performance
Separation-ESI Modules•

B07363 – CESI adapter for Sciex Nanospray III•

B07366 – CESI adapter for Thermo Nano Spray II•

B07367 – Silica Surface OptiMS Cartridge•

B11648 – CESI Vials•

B24699 – CESI Vial Caps•

144709 – Microvial•

5043467 – nanoVial

B07368
Contains 1 OptiMS Cartridge withthe pre-installed Neutral surface capillary[Please follow the Instructional Guide prior
to using the neutral capillary cartridge]

AB Sciex is doing business as SCIEX.
© 2015 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license.
RUO-MKT-07-2680-A 12/ 2015

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