C-Kit Ground Pro
High quality protein crystallization kit for XRD and ND
C-Kit Ground Pros are kits for growing high quality protein crystals. C-Kit Ground Pro XRD isfor X-ray diffraction and C-Kit Ground Pro ND for neutron diffraction. Use of C-Kit Ground Pro
allows you to optimize crystallization conditions easily in your lab.
ProductNoPrice

Itemexcluding tax

CRT101-1

C-Kit Ground Pro XRD1,2)¥50,400

CRT101-2

C-Kit Ground Pro ND3,4)¥151,200

CRT101-3

C-Kit Ground Pro e-mail support¥60,000

CRT101-4

C-Kit Ground Pro video support¥60,000

References:1)J. Synchrotron Rad. (2004) 11, 45.
2)Int. J. Microgravity Sci. Appl. (2019)36, 360107.
3)Crystals (2020) 10, 78.
4)Int. J. Microgravity Sci. Appl. (2021)
38, 380103.

These prices do not include sales tax and shipping fee.

C-Kit Ground Pro XRD1,2)
This kit contains two types of experimental tools; one is for the counter diffusion method (CD)and the other is for the diffusion pair under osmotic concentration method (DPOC). The
instructions for both methods are included.

CD1) is an established method for apo- and complex- protein crystal growth. It’s self-searchingmechanism increases the probability of generating high quality protein crystals. In addition, diffusion
process decreases the concentrations of co-existing small molecules around protein, so that thecrystallization conditions are less affected by contaminated chemicals in protein than the conventional
vapor diffusion method (VD). Seeding is also available in this method for the robust crystallization.

DPOC2) is a new method. In this method, the precipitant and the protein are tandemly loaded to form
a diffusion pair in a silicon tubing. They diffuse into each other and are osmotically concentrated atthe same time. As a result, protein is crystallized by both VD and CD mechanisms. DPOC isrecommended in the cases when the protein concentration is not optimal, the quantity of the ligand
for the co-crystallization is limited, or when CD generates no crystal. Seeding is also available in thismethod for the robust crystallization.
Item

QTY

CD

DPOC

Details

Capillary

15○

Glass capillary (ID 0.5 mm, OD 1.2 mm,length 47 mm).

Gel-tube

18○ID 1 mm, OD 2 mm, length 10 mm

5 mL test tube

23○

DPOC-tube○

For incubating loaded samples

8○

Silicon tube (ID 1.0 mm, OD 2.0 mm, length39.5 mm) without an osmo-resistant cover.

C-Cap

16〇

For protect DPOC-tube ends from osmoticpressure.

Silicon-tube

50cm

○

For sample loading

Instruction manual

1○○Sealing compound

1

○

○

C-Kit Ground Pro ND3,4)
To generate a single large protein crystal for ND, this kit contains experimental tools for successful
optimization of crystallization conditions in a large bore capillary (ID 2 mm) with a dialysis
membrane (LCDM). It contains narrow bore capillaries for the batch method (BT) and thecapillaries and the gel-tubes for the CD. CS recommends checking the crystallization behavior by BTat first to identify crystallization conditions, confirm and refine the crystallization behavior by CD, andfinally applying it to the LCDM trial.
ItemQty

BTCD

LCDM

Details

Capillary100○Capillary

15○

Glass capillary (ID 0.5 mm, OD 1.2mm, length 47 mm).

Gel-tube18○

ID 1 mm, OD 2 mm, length 10 mm

LCDM (glass)

6ID 0.29 mm, OD 1.15 mm, length 29mm

○ID 1.9 mm, OD 2.4 mm, length 18 mm
glass capillary with a dialysis
membrane (MWCO 6-8 kDa). GT is
attached already

LCDM (quartz)

2○

ID 2 mm, OD 2.4 mm, length 18 mm
Quartz capillary with a dialysis
membrane (MWCO 6-8 kDa). GT is
attached already

C-Cap

10

○

For protecting an end of LCDM fromosmotic pressure. Including 4 spares

Gel loading chip9○

For loading a sample into LCDM

5 mL test tube

30

○

○

○

For incubating loaded samples

Instruction manual1○○○Sealing compound

1○○○

On-line technical support
We provide on-line technical support on the concept of crystallization condition optimization andexperimental methods by e-mail or video meeting. If the biochemical information of the target
protein, crystallization conditions, and your results of crystal-experiment are disclosed, CS will identify
candidate countermeasures to obtain good crystals.
Items

Detailse-mail support

5 times QA

video support

1 hour

The methods and the devices of C-Kit Ground Pro
are compatible with the commercial Kirara service(https://www.jamss.co.jp/en/kirara/index.html)
andthe JAXA PCG service to use the microgravity
environment.

CONFOCAL SCIENCE INC.
MAIL：info@confsci.co.jp
http://www.confsci.co.jp/index_e.html

Musashino bld. Fukasawa 5-14-15, Setagaya-ku
Tokyo, 158-0081 Japan
TEL: +81-3-3864-6606 FAX: +81-3-6411-6261