Andor On-axis high throughput
HoloSpec imaging spectrograph• High collection efficiency ultrafast F/1.8aperture
Up to 6.5 times better light collection efficiency
than traditional 1/3rd m Czerny-Turner designs
100 % light collection from NA=0.22 fibre optics• On-axis imaging-corrected design
Superb optical aberration correction across alarge focal plane for superior spatial resolution
and high density, low crosstalk multi-track (multifibre) acquisitions
Gather more photons per pixel- increased signalto-noise ratio• High throughput optical design
High transmission volume phase holographic(VPH) gratings with state-of-the art optics maximum optical efficiency for visible or nearinfrared range• Low scattered light‘Smooth’ sinusoidal refractive index VPH gratings
profile greatly reduces stray light - maximizes
detection dynamic range and signal-to-noise• Compact and rugged design
Pre-aligned and pre-calibrated, out-of-the-box
operation, excellent thermal stability and easilytransportable• Easily interchangeable accessories‘Snap-in’ accessories, including precision slits
and pre-aligned grating assemblies• Specialized Raman grating optionsOptimized for Stokes/Anti-Stokes, low-frequency
or high-frequency Stokes operation, 514.5 to830 nm laser options• Optional integrated Rayleigh filtering unit
Fully-enclosed SuperNotch Plus Kaiser filter
compartment with user-friendly external
adjustment• Seamless integration with Andor’s world class
Spectroscopy detectors
Combine high optical throughput and ultrasensitive CCD, ICCD and EMCCDs cameras formaximum photon collection

Spectroscopy

Low Light Imaging

Features and Benefits

Gathering more photons... at pace!
Working with challenging photon fluxes? Need results in milliseconds, not minutes?
The superb light collection efficiency capabilities of the superfast F/1.8 Andor HoloSpec
spectrograph platform provides a perfect match to Andor’s ultra-sensitive CCD, EMCCD andICCDs detectors, offering the most sensitive and versatile detection solution on the market
for Visible or Near-Infrared spectroscopy.
The Andor HoloSpec is the ideal solution for collecting more light and achieving better
signal-to-noise ratio faster, which is critical for applications such as micro-Raman
mapping, microfluidics, real-time medical diagnosis (point-of-care analyzers) or stand-off
bacteriological agents or explosives detection.
The HoloSpec also offers aberration-corrected optics for excellent multi-track capabilities,
with high density fibre optics to enable simultaneous acquisitions with extremely low
crosstalk, even on narrow spectroscopy sensors.
Its rugged and compact design makes it an ideal tool for challenging industrial or in-the-field
applications, while still offering research-grade performance suitable for academic research.

Application focus
Chemical mapping – micro-Raman (e.g. SERS or TERS-based) or micro-fluorescence mapping Microfluidics – e.g. spectral flow cytometry In-vivo medical diagnosis
 Stand-off gated Raman or LIBS
 Process control1Andor On-axis high throughput
HoloSpec imaging spectrograph
Superior detection capabilities- 4 reasons to make every photon count2 Superb multi-track capabilities

the most sensitive
4 Choose
detectors on the market

Imaging-corrected optical system for superior,high density multi-channel spectroscopy (Seepage 3 for details).

iDus 416 ‘low-dark current deepdepletion’ CCD- superior near-IR detectionwith up to 95% QE at 800 nm.

image plane
spectrographsectionstray light gratings- higher
3 Low
dynamic range

Newton EMCCD- unmatched sensitivity
in the UV-visible range, superfast kHz
acquisition capabilities, single photon
sensitive.

entrance
slit

The ‘smooth’ refractive index structure of volume
phase holographic (VPH) gratings scatters lessunwanted light than the typical surface relief
structure of conventional ‘ruled’ gratings.

iStar ICCD- nanosecond-gated detector forrecording fast transient phenomena.

holographic
VPTTM grating

iDus 416

Newton EMCCD

iStar ICCD

1 Superior light gathering power- when every photon counts
HoloSpec

300 mmCZT spectrograph

320 mmCZT spectrograph

F/#

1.8

4.0

4.6

Numerical aperture (NA)

0.27

0.12

0.11

Cone angle (air)

30.1

14.3

12.4

Light gathering powercomparison-

F/4.0 Czerny-Turner
spectrographF/4.6 Czerny-Turner
spectrograph

Spectrographentrance plane

F/1.8 HoloSpec

Light collection varies with 1/(F/#) - the lower theF/# the higher the accepted light cone angle into thesystem, therefore the higher the collection power(see figure above).
2See technical note Andor
HoloSpec- the high throughput
spectrograph

HoloSpec is 4.9x better HoloSpec is 6.5x better

AND
HoloSpec gathers 100% of light from traditional silica-silica F/2.22(NA=0.22) fibre optics

Spectroscopy applications where throughput matters:

 Intrinsically photon-starved experiments...

e.g. Quantum dot photoluminescence, micro-Raman of biosamples, micro-photoluminescence of carbon nanostructures, plasmonics
spectroscopy of light harvesting complex or organic light-emitting diode (OLEDs), cathodoluminescence, stand-off chemical detection.

 When acquisition time is a constraint...

Gather enough photons in short periods of time while accessing meaningful signal-to-noise ratio.
e.g. micro-spectroscopy chemical mapping, micro-fluidics such as spectrally-resolved flow cytometry, on-line process control.

 Minimizing photodamage of photo-sensitive samples...

Protect samples from photodegradation and phototoxicity – achieve meaningful signal-to-noise ratio in shorter timescales to minimize overexposure to excitation sources e.g. biomaterials such as live cells or luminescent biotags.

2Andor On-axis high throughput
HoloSpec imaging spectrographExceptional high-density multi-track capabilities- for superior photon collection

Figure 1: Image of a very high density 19 x 100 μm core (125 μm inc. cladding) fibre
optic bundle at the output focal plane of a HoloSpec. Broadband source captured
from 532 - 609 nm with a Newton EMCCD DU971P-BV.

High-density multi-track AND high throughput
The HoloSpec advanced imaging corrected optics allow clear separation ofindividual channel images from densely packed fibre optics bundles.
Up to 32 x 100 μm core fibre channels can be individually resolved over a 4 mm
high sensor with low crosstalk despite the high density fibre bundle configuration.
Crosstalk can be further reduced by:••

Reading the sensor in multi-track mode with narrow track height
Using fibre bundle with alternating ‘Live’ and ‘Dead’ channels to offer zerocrosstalk between consecutive tracks.

Figure 2: Vertical intensity profile cross section of image
in figure 1 at centre and edges of the focal plane.

Throughput comparison: HoloSpec vs. CZ300 vs. CZ320 vs. CZ500
30

Advantages of full spectrum flow cytometry, C. K.
Sanders et al, J. Biomed. Opt. 18(3), 037004 (Mar 11,2013). doi:10.1117/1.JBO.18.3.037004 [2013]Same Input- Same Detector- Same Exposure
25

Fundamentals of stand-off Raman scattering spectroscopy
for explosive fingerprinting, J. Moros et al, J. Raman
Spectrosc., 44: 121–130. doi: 10.1002/jrs.4138 [2013]

Relative Intensity

20

Tracking circadian rhythms of bone mineral deposition inmurine calvarial organ cultures, J.-D. P. McElderry et al, J.
Bone Miner. Res., 28: 1846–1854. doi: 10.1002/jbmr.1924[2013]

15Holospec
CZ320

10Wrapping and dispersion of multiwalled carbon nanotubes
improves electrical conductivity of protein–nanotube
composite biomaterials, C. M. Voge et al, Journal of
Biomedical Materials Research Part A, 101A(1) 231–238. doi:
10.1002/jbm.a.34310 [2013]

CZ300
CZ500

50576.0

Holospec in action

576.5

577.0

577.5

578.0

578.5

579.0

579.5

580.0

Wavelength/nm

Figure 3: Direct comparison of HoloSpec throughput with Czerny Turner (CZT) systems.
Each with 1200 g/mm gratings optimized for the 500-600 nm region. Numbers refer tofocal lengths (mm).

In situ Raman spectroscopy for the evaluation of solubility insupercritical carbon dioxide mixtures, I. Rodriguez-Meizoso
et al, The Journal of Supercritical Fluids, 65: 87-92. doi:
10.1016/j.supflu.2012.03.002 [2012]
Synthesis of graphene-CoS electro-catalytic electrodes fordye sensitized solar cells, Santanu Das et al, Carbon, 50(13):4815–4821. doi: 10.1016/j.carbon.2012.06.006 [2012]

Have you found what you are looking for?
Need a higher spectral resolution? Andor’s motorised, research grade Shamrock Czerny-Turner spectrographs offer 500 &750 mm focal lengths.
Need to work in the SWIR regions? Andor’s Kymera and Shamrock series can be configured with silver-coated optics forenhanced collection efficiency in the NIR-SWIR with Andor iDus InGaAs detectors.

3

Andor On-axis high throughput
HoloSpec imaging spectrograph
HoloSpec F/1.8- ideal for general broadband Spectroscopy
Parameter

HOLOSPEC-F/1.8-VIS

HOLOSPEC-F/1.8-NIR

Optimized operation wavelength (nm)450-730

800-1,060

F/# aperture

F/1.8 (across entire plane)

F/1.4 @ centre
F/1.8 @ edges

Focal length(output/input, mm)

85/75

85/75

Magnification

1.13

1.13

32415Dimensions L x W x Hmm [inches]

250 x 190 x 170[10 x 7.5 x 6.7]

Weight kg [lbs]

5 [11]

6Key
1Base unit

HOLOSPEC-F/1.8-VIS – Visible range
HOLOSPEC-F/1.8-NIR – Near IR range

2Gratings

HS-H**-*** - See ‘Broadband’ volume phase holographic gratings table on page 6 for Broadband orRaman-specific options

3Detector

Please refer to the iDus CCD, Newton CCD & EMCCD and iStar ICCD specification sheets to select thebest detector for your wavelength range and desired resolution

4Detector flange

HS-FLG-CCD – For Spectroscopy CCD, EMCCD and ICCD detectors
HS-SHT-9005 - Ø35 mm shutter with flange assembly for Spectroscopy CCD, EMCCD and ICCD
detectors (shutter driver ACC-SD-VED24 must be ordered separately)

5Input accessories

HS-EXSLIT-INAD– Entrance slit mount for input slit (one required per system with a slit input
configuration)
HS-SLT-INPUT-**** – Entrance slits, (require one HS-EXSLIT-INAD to be ordered per system), See Inputs& Intermediate Slits table on page 6 for available options
HS-FOI-FC - FC fibre adapter
HS-FOI-SMA - SMA fibre adapter
HS-ASM-8081 - Ø11 mm fibre ferrule X-Y adjuster (cannot be used in conjunction with input slit)
HS-ASM-0101 - 30 mm cage adapter input
Ferrule multi-track fibre adapters•16Additional input accessories

Detector
Specification sheets
andor.com/spectroscopy

Integrated Raman probes•1Resolution calculator
andor.com/calculators

4

Andor On-axis high throughput
HoloSpec imaging spectrograph
HoloSpec F/1.8i- ideal for Raman applications
Parameter

HOLOSPEC-F/1.8i-VIS

HOLOSPEC-F/1.8i-NIR

Optimized operation wavelength (nm)450-730

800-1,060

Integrated Notch filter chamberYes

F/# aperture

F/1.8 (across entire plane)

F/1.4 @ centre
F/1.8 @ edges

Focal length(output/input, mm)

85/75

85/75

Magnification

1.20

1.20

435621Key

78Dimensions L x W x Hmm [inches]

440 x 190 x 170[17.3 x 7.5 x 6.7]

Weight kg [lbs]8.2 [18]

HOLOSPEC-F/1.8i-VIS – Visible range
HOLOSPEC-F/1.8i-NIR – Near IR range

1Base unit

2Notch filters

3Gratings

HS-H**-*** - See ‘Raman’ volume phase holographic gratings table on page 7 for Raman-specific options

4Detector

Please refer to the iDus CCD, Newton CCD & EMCCD and iStar ICCD specification sheets to select the best
detector for your wavelength range and desired resolution

5Detector flange

HS-FLG-CCD – For Spectroscopy CCD, EMCCD and ICCD detectors
HS-SHT-9005 - Ø35 mm shutter with flange assembly for Spectroscopy CCD, EMCCD and ICCD detectors(shutter driver ACC-SD-VED24 must be ordered separately)

6

Intermediate accessories

HS-SLT-INTER -**** – Intermediate slits, See Input & intermediate slits table on page 6 for available options

7Input accessories

HS-EXSLIT-INAD– Entrance slit mount for input slit (one required per system with a slit input configuration)
HS-SLT-INPUT-**** – Entrance slits, (require one HS-EXSLIT-INAD to be ordered per system), See Input &intermediate slits table on page 6 for available options
HS-FOI-FC - FC fibre adapter,
HS-FOI-SMA - SMA fibre adapter
HS-ASM-0101 - 30 mm cage adapter input
Ferrule multi-track fibre adapters•18Additional input accessories

HS-HSPF-*** - See Notch Filters table on page 6 for specific laser wavelength options

Integrated Raman probes•1

Detector
Specification sheets
andor.com/spectroscopy

Resolution calculator
andor.com/calculators

5

Andor On-axis high throughput
HoloSpec imaging spectrograph
Input & intermediate slits
Slit size (W x H) •2, 3

Input slit part number

Intermediate slit part number(‘i’ models only with Rayleigh filtering compartment)

25 μm x 8 mm

HS-SLT-INPUT-0025

HS-SLT-INTER-0025

50 μm x 8 mm

HS-SLT-INPUT-0050

HS-SLT-INTER-0050

100 μm x 8 mm

HS-SLT-INPUT-0100

HS-SLT-INTER-0100

250 μm x 8 mm

HS-SLT-INPUT-0250

HS-SLT-INTER-0250

500 μm x 8 mm

HS-SLT-INPUT-0500

HS-SLT-INTER-0500

1000 μm x 8 mm

HS-SLT-INPUT-1000

HS-SLT-INTER-1000

2000 μm x 8 mm

HS-SLT-INPUT-2000

HS-SLT-INTER-2000

4000 μm x 8 mm

HS-SLT-INPUT-4000

HS-SLT-INTER-4000

Notch filters
Laser wavelength (nm) •3DiameterOptical Density at laser
wavelength

Spectral bandwidth (cm-1)

514.5

Andor part number
HS-HSPF-514.5

532Ø 50 mm (2”)

HS-HSPF-532.0< 350>6632.8

HS-HSPF-632.8

785

HS-HSPF-785.0‘Broadband’ volume phase holographic gratings*
Central wavelength(nm)•3Nominal dispersion(nm/mm)

590

15.78

600

Nominal Bandpass(nm)[λ min, λ max]•6,Resolution at centre(nm)•7,8

Andor part number

Recommended
Holospec model

372 to 808 nm•40.95HS-HVG-590

VIS11.15446 to 754 nm

0.67

HS-HFG-600

VIS

650

11.92485 to 815 nm

0.72

HS-HFG-650

VIS730.8

13.46545 to 917 nm

0.81

HS-HFG-730.8

VIS75013.85559 to 942 nm

0.83

HS-HFG-750

NIR

800

21.54502 to 1098 nm•51.29HS-HVG-800

NIR85015.77

632 to 1068 nm•50.95HS-HFG-850

NIR* values shown for a 50 µm x 4 mm (W x H) slit

6

40.7

67.1

27.7

High-frequency

Stokes Anti-Stokes

Stokes Anti-Stokes(high dispersion)

Low-frequency

High-frequency

Stokes Anti-Stokes

Low-frequency

High-frequency

Stokes Anti-Stokes

Low-frequency

High-frequency

Stokes Anti-Stokes

Low-frequency

High-frequency

Stokes Anti-Stokes

Stokes Anti-Stokes(high dispersion)

Low-frequency

High-frequency

Stokes Anti-Stokes

Low-frequency

High-frequency

514.5

532

532

532

532

632.8

632.8

632.8

647

647

647

752

752

752

785

785

785

785

830

830

830

* values shown for a 50 µm x 4 mm (W x H) slit

97.5

Low-frequency

514.5

56.9

66.5

74.7

60.5

75.4

62.3

65.3

69.7

67.0

76.4

81.1

68.3

77.9

83.6

81.5

91.9

84.6

96.9

101.0

Stokes Anti-Stokes

514.5

Average reciprocal
dispersion (cm-1/mm)

Specific coverage

Laser wavelength•3(nm)

6.24

5.34

5.11

5.87

5.36

1.69

4.16

5.58

4.29

3.99

4.16

3.65

3.43

4.08

3.57

3.33

3.42

2.97

1.14

2.86

3.29

2.87

2.76

Nominaldispersion (nm/mm)

Nominal Bandpass(cm-1) [shift min,shift max] •61685 to 3257 cm-1

-67.9 to 1771 cm-1-1117 to 947 cm-1

1718 to 3390 cm-1

-207 to 1877 cm-1

-460 to 307 cm-1-919 to 936 cm-1

1835 to 3558 cm-1

24.7 to 1830 cm-1-915 to 1012 cm-1

1805 to 3656 cm-1

-115 to 1997 cm-1

-1070 to 1171 cm-1

1882 to 3770 cm-1

-84 to 2071 cm-1

-1240 to 1072 cm-1

2178 to 4432 cm-1

-111 to 2430 cm-1

-673 to 453 cm-1

-1082 to 1614 cm-1

2201 to 4539 cm-1

-312 to 2367 cm-1

-1160 to 1634 cm-1

‘Raman’ volume phase holographic gratings*

965 to 1138 nm•5825 to 973 nm

760 to 901 nm

908 to 1070 nm•5773 to 921 nm

758 to 804 nm

732 to 847 nm

872 to 1027 nm

753 to 872 nm

704 to 814 nm

733 to 848 nm

642 to 743 nm

605 to 700 nm

718 to 831 nm

630 to 728 nm

587 to 679 nm

602 to 696 nm

529 to 611 nm

514 to 545 nm

503 to 582 nm

583 to 675 nm

507 to 590 nm

487 to 566 nm

Nominal
Bandpass (nm)[λ min, λ max]•6

3.41

3.99

4.48

3.63

4.52

1.66

4.03

3.74

3.92

4.18

4.02

4.58

4.86

4.10

4.68

5.02

4.89

5.51

2.44

5.85

5.07

5.81

6.06

Average resolution•7,8(cm-1)

0.37

0.32

0.31

0.35

0.32

0.10

0.25

0.34

0.26

0.24

0.25

0.22

0.21

0.25

0.21

0.20

0.21

0.18

0.07

0.17

0.20

0.170.17

Resolution at•7,8
centre (nm)HS-HSG-830-HF

HS-HSG-830-LF

HS-HSG-830-SA

HS-HSG-785-HF

HS-HSG-785-LF

HS-HDG-785

HS-HSG-785-SA

HS-HSG-752-HF

HS-HSG-752-LF

HS-HSG-752-SA

HS-HSG-647-HF

HS-HSG-647-LF

HS-HSG-647-SA

HS-HSG-632.8-HF

HS-HSG-632.8-LF

HS-HSG-632.8-SA

HS-HSG-532-HF

HS-HSG-532-LF

HS-HDG-532

HS-HSG-532-SA

HS-HSG-514.5-HF

HS-HSG-514.5-LF

HS-HSG-514.5-SA

Andor part number

NIR

NIR

NIR

NIR

NIR

NIR

NIR

NIR

NIR

NIR

NIR

VISVIS

NIR

VIS

VIS

VIS

VIS

VIS

VIS

VIS

VIS

VIS

Recommended
Holospec model

Andor On-axis high throughput
HoloSpec imaging spectrograph7

Andor On-axis high throughput
HoloSpec imaging spectrograph

Order Today
Need more information? At Andor we are committed to finding
the correct solution for you. With a dedicated team of technicaladvisors, we are able to offer you one-to-one guidance andtechnical support on all Andor products. For a full listing of ourandor.com/contact
regional sales offices, please see:
Our regional headquarters are:Europe 				JapanBelfast, Northern Ireland 			Tokyo
Phone +44 (28) 9023 7126			
Phone +81 (3) 6732 8968
Fax +44 (28) 9031 0792			
Fax +81 (3) 6732 8939
North America				China
Concord, MA, USA 				Beijing
Phone +1 (860) 290 9211			
Phone +86 (10) 8271 9066
Fax +1 (860) 290 9566			
Fax +86 (10) 8271 9055
Items shipped with your Spectrograph
1x Spectrograph base unit (with integrated
Notch compartment for ‘i’ models), including
set of 4 clamping feet
1x Grating fitted as selected at time of ordering
1x Input accessory (slit or fibre-opticsconnector) fitted as selected at time ofordering
1x Detector flange fitted as selected at timeof ordering
1x User guide and Quick Start Guide (on CD)
1x Individual performance sheet

Footnotes: Specifications are subject to change without notice1.

Please contact your Andor representative to discuss available options

2.

For alternative slit height options, please contact your local Andor representative

3.

Special designs are available on request - please contact your local Andor representative

4.

The HoloSpec transmission decreases rapidly below 400 nm, so the full wavelength range displayed may
not be achievable

5.

Silicon-based detectors are sensitive to around 1,050 nm, so the full wavelength range displayed will not beachievable

6.

Typical values quoted for a 27.6 mm wide sensor, e.g. Newton DU940

7.

Typical values quoted for a 50 µm x 4 mm (W x H) slit and a 13.5 µm pixel sensor, e.g. Newton DU940

8.

For F/1.8i models, typical resolution should be multiplied by 1.2 due to the optical magnification of thesystem.

Operating & Storage Conditions• Operating Temperature: 10°C to 40°C ambient• Relative Humidity: < 80% (non-condensing)• Ingress Protection: IP20
• Storage Temperature: -20°C to 70°C

FM40523

EMS91062

OHS502497

HSPECSS 1217 R18