Anti- Salmonella enteritidis LPS antibody, mouse monoclonal (se-1)
Product code64-018Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium

Immunogen

Crude extract of Salmonella Enteritidis

Isotype

Mouse IgG1

Reactivity

Reacts with LPS of Salmonella enteritidis and Salmonella typhimurium. Does
not react with other gram-negative food-poisoning bacteria like E. coli, V.

parahaemolyticus and Campylobacter species.
Special notes

N/A

Application

1. Western blotting (1/1000~1/2000 )2. ELISA (assay dependent)
3. Immunochromatography (assay dependent)
Other applications have not been tested.

Background

Salmonella enterica subsp. enterica serotype Enteritidis (SE) is one of the majorcausative agents of human gastroenteritis. Salmonella enterica subsp. enterica
is classified into over 1500 serotypes based on antigenic differences inlipopolysaccharide (LPS) (O) and flagellar (H) antigens. LPS is a major
component of the outer surface of gram-negative bacteria, composed of ahydrophobic lipid A, which anchors LPS to the membrane, a core oligosaccharideregion, and an O-polysaccharide polymer (O-chain) composed of oligosacchariderepeating units. While the LPS-core regions are relatively conserved among
gram-negative organisms, there is a substantial difference in the composition ofthe O-chain repeating units, which leads to a large antigenic diversity in Oantigens.

Data Link

N/A

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

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Data Images: 64-018 Anti-Salmonella enteritidis LPS antibody, mouse monoclonal (se-1)

Fig.1. Western blotting of LPS from S. Enteritidis with MAb (se-01).
1.

Crude extract of S. Enteritidis cells

2.

Purified LPS of S. Enteritidis (Sigma-Aldrich),

Crude extract and purified LPS (1 µg) were loaded and separated by SDS-PAGE gel and blotted onto
nitrocellulose membrane. After blocking with 5 % skim milk, membrane was reacted with MAb at
1/250 dilution. They showed similar band patterns characteristic of LPS, O-chain repeating unit. MAb(se-01) recognizes common antigenic determinants that are found in the structural components ofSalmonella LPS.

Fig 2. ReactivityDot of MAb (se-01) with several food poisoning bacteria in Western blotting (A) andslot blot test (B).
(A) 1: S. Enteritidis, 2: Vibrio parahaemolyticus, 3: Escherichia coli 4: E. coli O157:H7. MAB (ae-01)
reacts only with S. Enteritidis.
(B) Extracts of each strain of food poisoning bacteria were coated onto 5 area of a nitrocellulose
membrane. Each membrane was soaked and reacted with MAb (se-01). SE: S. Enteritidis, EC: E.
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coli,CJ: Campylobacter jejuni, VP: V. parahaemolyticus, MAb (se-01) recognizes LPS of S.

Enteritidis, but does not react with any non-Salmonella bacteria tested.

Fig.3. Titration of antibody reactivity of MAb (se-01) by indirect ELISA using crude extract of S.

Enteritidis
The wells of plate were coated with crude extract of S. Enteritidis (100 µl, 1 μg/ml). After blocking
with 5% skim milk, 100 µl of antibody at the indicated dilution was added to the each well. HRPconjugate goat anti-mouse IgG (100μl 、 x2000 dilution) was added. Color was developed withorthophenylenediamine as substrate. Optical densities (OD) measured at 490nm.

Fig4. Titration of LPS in the extract of S. Entiritidis cells by indirect ELISA .
ELISA plate was coated with indicated amounts of the extract of S. Enteriditis cells . MAB (se-01)
was used at 1/500 dilution. ELISA was performed as described in Fig.3

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Fig.5 Immunochromatography with MAb (se-01)
Goat anti-S.Enteritidis serum and rabbit anti-Campylobacter jejuni serum

were coated onto a

specific upper area and under area, respectively, of the same nitrocellulose membrane, while goatanti-mouse IgG was coated onto another specific area (control line) on the same membrane. MAb (se01) or C. jejuni MAb conjugated with colloidal gold were mixed with extract of each food poisoning
bacteria in well. The strips were soaked and reacted with the mixture.
Specific reactivity was shown in the well containing S. Enteritideis (left strip) and C. jujeni (rightstrip).

Table 1. Reactivity of MAb (se-01) with various food poisoning bacteria.

Reference: There has been no publication using this antibody.
Please let us know when your research using this antibody is published so that we can cite thepublication in this datasheet. We will offer one vial of our antibody as compliment.

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