The Promoter Activities of Reporter Constructs
HepG2 cells

74.5

15.4

Relative Light Units (RLU)

15.6

4.0
3.5
3.0
2.5
2.0
1.5

15.2
1.2
1.0
0.8
0.6
0.4

0.5

0.2

0.0

0.0

Po
si.
Neg
a
M .
M
P2IF
N
G
IN
PL S
A
U
R
PL
A
T

1.0

Po
si.
N
eg
a
M .
M
P2IF
NG
IN
PL S
A
U
R
PL
A
T

Relative Light Units (RLU)

HeLa cells
75.0

Hep3B cells
39.0
5.0
4.0
3.0
2.0
1.00.0Posi.: pGL3 Control Vector(SV40 promoter)
Nega.: Empty Vector
MMP2: pGL4-phMMP2 (RDB#7314)
IFNG: pGL4-phIFNG(RDB#7297)
INS:pGL4-phINS (RDB#7387)
PLAUR:pGL4-phPLAUR (RDB#7312)
PLAT:pGL4-phPLAT (RDB#7295)

Posi.
Nega
M .
M
P2IF
N
GIN
PL S
A
U
R
PL
A
T

Relative Light Units (RLU)

40.0

2009, DNA Bank, RIKEN BioResource Center[ GENETYX : Nucleotide Sequence Homology Data ]Date : 2010.01.25
1st Nucleotide Sequence
File Name: Reference Seq.gnu

Sequence Size: 5425

2nd Nucleotide Sequence
File Name: RDB7314F.fasta

Sequence Size: 735

Unit Size to Compare = 1
Pick up Location[93.333% / 690 bp]= 1

INT/OPT.Score : <354/ 2310 >1'GGCCTAACT GGCCGGTACC*1" AAATGGGAAA GCCCTGACAT AATCAACACT CGATCTTTGC GAGAAACGCN TAAAACCCCA
20' TGAGCTCGCT AGCCTCGAGG ATTTCCTCAG AGACGGTTGT CACAGGGAGA ACTNTCAAAA***** *** * ***.*** *** ***** *********** ***.******

61" TGACGTCACA GTCCACAGGT ATNTCCACAG AGACGTTGTA CACAGGGAGA ACTTTCAAAA
80' TTGGGATTCG ACCTGAGAGG CCACATGGAT TCTTGGCTTG GCGCNAGGAA AGGATTCAAG********** ********** ********** ********** **** ***** **********
121" TTGGGATTCG ACCTGAGAGG CCACATGGAT TCTTGGCTTG GCGC-AGGAA AGGATTCAAG
140' AGTGAGTGGG GAATTCGTGG AACTGAGGGC TCCTCNCCCT TTTTAGACCA TATAGGGTAA********** ********** ********** ***** **** ********** **********
180" AGTGAGTGGG GAATTCGTGG AACTGAGGGC TCCTC-CCCT TTTTAGACCA TATAGGGTAA200' ACCTCCCCAC ATTGCCATGG CATTTANTAA ACTGCCATGG CACTGGTGGG TGCTTCCTTT********** ********** ****** ************ ********** **********

239" ACCTCCCCAC ATTGCCATGG CATTTA-TAC ACTGCCATGG CACTGGTGGG TGCTTCCTTT2010 DNA Bank, RIKEN BioResource Center

260' AACATGCTAA TGCATTANTA ATTAGCGTAA AATGAGCAGT GAGGATGACC AGAGGTCGCT********** ******* ** ********** ********** ********** **********298" AACATGCTAA TGCATTA-TA ATTAGCGTAA AATGAGCAGT GAGGATGACC AGAGGTCGCT320' TTCTTTGCNC ATCTTGGTTT TGGCTGGCTT CTTCACTGCA TACTGTTTTA TCAGTGGGGN******** * ********** ********** ********** ********** *********
357" TTCTTTGC-C ATCTTGGTTT TGGCTGGCTT CTTCACTGCA TACTGTTTTA TCAGTGGGG380' TCTTTGTGAC CTCTATCTTA TTAAACCAGT CTTG-CCCAA TTTCTATCTC ANTCCTGTGA********** ********** ********** **** ***** ********** * ********415" TCTTTGTGAC CTCTATCTTA TTAAACCAGT CTTGCCCCAA TTTCTATCTC A-TCCTGTGA
439' CCGAGAATGC GGACCCTCCT GGGAGTGCAG CCCAGCAGGT CTNCAGCCTC ATTTTACCCA********** ********** ********** ********** ** ******* **********
474" CCGAGAATGC GGACCCTCCT GGGAGTGCAG CCCAGCAGGT CT-CAGCCTC ATTTTACCCA
499' GCCCCCTGTT CAAGATGGAG TCGCTCTGGT TCCNAACGTC TCTAACGCGG GGCCCCTGAC********** ********** ********** *** ****** ********** ** *******533" GCCCCCTGTT CAAGATGGAG TCGCTCTGGT TCC-AACGTC TCTAACGCGG GGGCCCTGAC
559' TGCTCTATTT CCCAAGGTGT ATCTNAGCAT CTCGCACTAT ACGAGGCCAA GTTAAGGCTT******* ** *** ****** **** ***** ********** ****** *** *********592" TGCTCTA-TT CCCCAGGTGT ATCT-AGCAT CTCGCACTAT ACGAGG-CAA GTTAAGGCTA
619' ACACATTTGC AGAAGNGAAA GAGGTAAGGA AGCAACCTGG GACCTTCCAC TGTCTCTGTT********** ***** **** ***** **** ********** **** ***** **********649" ACACATTTGC AGAAG-GAAA GAGGT-AGGA AGCAACCTGG GACC-TCCAC TGTCTCTGTT679' TCCATCNTCT CTCTTTCCAT CTCTGTTCAT CCCAGAATCT CTCTGTCCCT ATCCCTANAA* **** *** ***** **** * **706" T-CATC-TCT CTCTTCCCTC CTGTTCTCCA GA

1st Nucleotide Sequence
File Name: Reference Seq.gnu

Sequence Size: 5425

2010 DNA Bank, RIKEN BioResource Center2nd Nucleotide Sequence
File Name: RDB7314R.fasta (Complementary)

Sequence Size

: 185

Unit Size to Compare = 1
Pick up Location[91.979% / 187 bp]= 1

INT/OPT.Score : <330/661 >

1081' GTAGGGGGGT GGNGGCAGAG AGGGGCGGGC CCGAGTGCGC CCCCCGCCCC CAGCCCCGCT.. ........** **********
1"

NN NNNNNNNNCC CAGCCCCGCT

1141' CTGNCCAGCT CCCTCCCAGC CCAGCCGGCT ACATCTGGCG GCTGCCCTCC CTTGNTTTCC*** ****** ********** ********** ********** ********** **** *****
23" CTG-CCAGCT CCCTCCCAGC CCAGCCGGCT ACATCTGGCG GCTGCCCTCC CTTG-TTTCC
1201' GCTGCATCCA GACTTCCTCA GGCGATCAAG ATCTGGCCTC GGCGGCCAAG CTTGGCAATC********** ********** ********** ********** ********** **********
81" GCTGCATCCA GACTTCCTCA GGCGATCAAG ATCTGGCCTC GGCGGCCAAG CTTGGCAATC
1261' CGGTACTGTT GGTAAAGCCA CCATGGAAGA TGCCAAAAAC ATTAAGAAGG GCCCAGCGCC********** ***. * *** ********** ********** *****
141" CGGTACTGTT GGTNGA-CCA CCATGGAAGA TGCCAAAAAC ATTAAA

2010 DNA Bank, RIKEN BioResource Center