Anti- DSN1 / Mis13 antibody, rabbit serum
Product code70-101

Size100µl

Storage

Store at 4℃ for short term. For long term storage store at -20℃.
Aliquot to avoid repeated freezing thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityAnti-GST antibodies have been removed from the rabbit antiserum with affinity
column conjugated with GST

Immunogen

Recombinant GST-DSN1 (1-356 aa)

IsotypeRabbit IgG

Reactivity

Human, mouse and rat

Special notes

Specificity has been validated with siRNA of human DSN1 for WB and IF.

Application1) Western blotting (1,000 fold dilution)2) Immunofluorescence-staining (1,000 fold dilution,)

Background

DSN1 / Mis13 (human: 356 aa. 40 kDa) is part of the MIS12 complex which is
required for normal chromosome alignment and segregation and kinetochore
formation during mitosis. Defects in kinetochore proteins often lead toaneuploidy and cancer. It is a component of the MIS12 complex composed ofMIS12, DSN1, NSL1 and PMF1 and also interacts with CASC5, CBX3 andCBX5. Note that DSN1 protein is expressed in actively dividing cells.

Data LinkUniProtKB Q9H410 (DSN1_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-101 Anti- DSN1 / Mis13 antibody, rabbit serum

Fig.1 Identification of endogenous DSN1 protein in the crude lysate of MCF-7 cells by using anti-DSN
antibody.
The cell lysate (20 μg) was separated by SDS-PAGE and analyzed by western blotting withanti-DSN antibody at 1/1,000 dilution. As the second antibody, anti-rabbit IgG antibody conjugatedwith HRP (Abcam, ab97051) was used at 10,000 dilution.

Fig.2 Validation of specificity of the anti-DSN1 antibody in WB by using siRNA. Expression of siRNAfor DSN1 for 24 h greatly reduced DSN1 in HeLa cells. Luciferase siRNA was used as a control.
Antibody was used at 1/1,000 dilution.

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Fig.3 Immunofluorescence staining of HeLa cell chromosome with this antibody and validation ofspecificity with siRNA. Expression of siRNA for DSN1 for 24 h greatly reduced DSN expression in thecell. Luciferase siRNA was used as a control. Antibody was used at 1/1,000 dilution. Mitotic cells wereaccumulated by nocodazole treatment and chromosomes were spread on coverglass.

Fig.4 Immunofluorescence stainig of DSN1 in HeLa cells using anti-DSN1 antibody.
The cells were fixed with 4% paraformaldehyde. The antibody was used at 1/100 dilution. As thesecond antibody, Alexa Fluor conjugated goat anti rabbit IgG was used at 1/1,000 dilution. Nuclear
DNA was stained with DAPI and the merged image was shown on the right.
Note that DSN1 is abundantly expressed in mitotic cells on mitotic apparatus.
Reference: This antibody has been used in the following reference
Obuse C et al “A conserved Mis 12 centromere complex is linked to heterochromatic HP1 and outerkinetochore protein Zwint-1“ Nat Cell Biol 6: 1135-1141 (2004) PMID: 15502821

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