Anti-Laminin alpha-3 antibody, mouse monoclonal (BM515)
Product code

70-350Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Native laminin α-3 chain from bovine cornea

Isotype

Mouse IgG1κ

Reactivity

Laminin α-3 (Human, Rabbit, Bovine)
No reactivity against other laminin chains

Special notes

N/A

Application

1. Western blotting: x1/1,000-2,500 (Fig.1)
2. Immunofluorescence microscopy x1/250-500 (Fig.2,3)

Background

Laminins, a family of extracellular matrix glycoproteins, are the major
noncollagenous constituent of basement membranes. Laminins have been
implicated in a wide variety of biological processes including cell adhesion,differentiation, migration, signaling, neurite outgrowth and metastasis, byinteracting with other extracellular matrix components. Laminins areheterotrimeric proteins with a high molecular mass (~400 to ~900 kDa). They
contain three different chains (α, β and γ) encoded by a distinct genes,respectively. Laminin subunit α-3 in humans is encoded by the LAMA3 gene,
known as 165 kDa subunit, and thought to be involved in cell adhesion, signaltransduction and differentiation of keratinocytes.

Data Link

UniProtKB: Q16787 (LAMA3_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

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Data Images: 70-350 Anti-Laminin alpha-3 antibody, mouse monoclonal (BM515)Fig. 1 Western blot analysis of BM515 antibody
Conditioned medium prepared from DJM-1 cells was concentrated by ammonia sulfate precipitation,
and was stained with CBB and immunoblotted with BM515 antibody (1:2,500 dilution). The HRPconjugated goat anti-mouse IgG was used as the second antibody. This antibody detected 165-kDa
band, a processed form of lamininα-3 chain. Protein bands were visualized using a chemiluminescentdetection with EzWestLumi plus kit (ATTO, Tokyo, Japan).

Fig.2 Immunofluorescence microscopy of human skin
A frozen acetone-fixed human skin section was stained with BM515 antibody (1:500 dilution). FITCconjugated goat anti-anti-mouse IgG as second antibody. The antibody revealed the location oflaminin-3 chain at the dermal-epidermal junction (arrows). E: epidermis, D: dermis. Bar = 50 m.

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Fig. 3 Immunofluorescence microscopy of DJM-1 cells
Methanol-fixed human carcinoma derived DJM-1 cells were stained with BM515 antibody (1:500dilution, green) and DAPI (blue). The FITC-conjugated goat anti-mouse IgG was used as the secondantibody. The antibody detected arc- or spotted staining patterns (arrows), which are typical fordeposited laminin-332 in DJM-1 cells. Bar = 20 m.
Reference
1. Hirako Y., Yoshino K., Zillikens D., Owaribe K. Extracellular cleavage of bullous pemphigoid
antigen 180/type XVII collagen and its involvement in hemidesmosomal disassembly. J Biochem.
133: 197-206, 2003
2. Uematsu J., Nishizawa Y., Hirako Y., Kitamura K., Usukura J., Miyata T., Owaribe K. Bothtype-I hemidesmosomes and adherens-type junctions contribute to the cell-substratum adhesion
system in myoepithelial cells. Eur J Cell Biol. 84: 407-415, 2005.
3. Owaribe K, Nishizawa Y, Franke WW. Isolation and characterization of hemidesmosomes frombovine corneal epithelial cells. Exp Cell Res. 192:622-630, 1991.
Related product70-352 Anti-Laminin ganmma-2 antibody, mouse monoclonal

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/