Anti-Amyloid Precursor Protein (APP C-terminus) antibody, rabbit serum (AC1)
Product code74-104

Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

ImmunogenSynthetic peptide corresponding to the C-terminus (aa 671-695) of human
APP695.

IsotypeRabbit IgG

Reactivity

Human, mouse and rat APP.

Special notes

N/AApplication

1. Western blotting (dilution: 1/3,000)
2. Immunocytochemistry (dilution: 1/1,000)
3. Immunohistochemistry (dilution: 1/500)
Other applicacatiotons have not been tested.

Background

The Alzheimer amyloid precursor protein (APP) is an integral membraneprotein expressed in many tissues and concentrated in the synapses of neurons.
Its primary function is not known, though it has been implicated as a regulatorof synapse formation and neural plasticity. APP is best known and mostcommonly studied as the precursor molecule whose proteolysis generates
amyloid beta (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form
is the primary component of amyloid plaques found in the brains of Alzheimer’s
disease patients. Isoform APP695 lacking the protease inhibitor domain is thepredominant form in neuronal tissues. An antibody (named AC1) against the Cterminus of human APP695 (aa 671-695) was raised in rabbit (2).

Data Link

UniProtKB P05067 (A4_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 74-104 Anti-Amyloid Precursor Protein (APP C-terminus) antibody, rabbit serum (AC1)

Fig.1 Endogenous expression of APP in mouse P19 cells during neural differentiation was analyzed
by Western blotting using this antibody (ref.2).
lane1, undifferentiated P19 cells (U); lane2, day 3; lane3, day4; lane4, day7; lane5, day12; lane6,day15; lane7, day17.
APP species with 105-120K markedly increased during days 3-12, but declined thereafter. On theother hand, APP species with 115-130K were detected on day 15 and 17.

Fig.2 Immunocytochemistry for APP (ref 4).
Mouse dorsal root ganglion neurons were cultured in the presence of nerve growth factor (NGF), fixed
at indicated time points, and immunostained for the C-terminus of APP with this antibody. Leftpanels are differential interference contrast images of the same fields. APP immunoreactivity wasvery low at 0 h but increased in neuronal somata (arrows), neurites (arrowheads) 3-12 h after NGF
treatment, a period when neurites showed a marked outgrowth. APP immunoreactivity decreased at36 h.
2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

References: This antibody has been used in ref.2, 3, 4 and 5.
1. Kang HG et al. (1987) “The precursor of Alzheimer’s disease amyloid A4 protein resembles a cellsurface receptor.”Nature 325: 733-736 PMID: 2881207
2. Yoshikawa K et al. (2002) ”Degeneration in vitro of post-mitotic neurons overexpressing theAlzheimer amyloid protein precursor.” Nature 359: 64-67 (1992) PMID: 1301020
3. Nishimura I et al. (2002) “Cell death induced by a caspase-cleaved transmembrane fragment ofthe Alzheimer amyloid precursor protein.” Cell Death Differ. 9: 199-208 PMID: 11840170
4. Nishimura I et al. (2003) “Upregulation and antiapoptotic role of endogenous Alzheimer amyloid
precursor protein in dorsal root ganglion neurons.” Exp. Cell Res. 286: 241-251 PMID: 12749853
5. Nishimura I et al. (1998) “Degeneration in vivo of rat hippocampal neurons by wild-typeAlzheimer amyloid precursor protein overexpressed by adenovirus-mediated gene transfer.” J.
Neurosci. 18: 2387-2396 PMID: 9502800
Related products
74-102 Anti-activated Caspase 3 (p20/p17 subunit) antibody, rabbit serum (ACP3)
74-106 Anti-Amyloid Precursor Protein (APP N-terminus) antibody, rabbit serum (AN2)74-108 Anti-APP-C31 (C-terminal fragment of the caspase 3-cleaved APP) antibody, rabbit serum(ACT1)
74-110 Anti-APPΔC31 (specific to C-terminal APP Δ31) antibody, rabbit serum (SAC)

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/