Anti-Dlx2 antibody, guinea pig serum
Product code74-116

Size

50 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.09% sodium azide

PurityGuinea pig antiserum

ImmunogenPurified recombinant MBP-Dlx2 (amino acids 1-154) protein.

Isotype

Guinea pig IgG

Reactivity

Reacts with mouse, marmoset, humanSpecial notes

N/A

Application

1. Western blot (1/1,000)
2. Immunofluorescent/Immunocytochemical staining
3. Immunohistochemistry (1/2,000-1/3,000)
4. Chromatin-ImmunoPrecipitaiton (assay dependent)

BackgroundActs as a transcriptional activator (PubMed:21875655). Plays a role in terminal
differentiation of interneurons, such as amacrine and bipolar cells in thedeveloping retina (PubMed:21875655). Likely to play a regulatory role in thedevelopment of the ventral forebrain (PubMed:1678612). May play a role incraniofacial patterning and morphogenesis (PubMed:1678612).

Length:332

amino acids. Mass (Da): 34746
Subcellular localization: Nucleus.

Data Link

UniProtKB P40764(DLX2_MOUSE)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 74-116 Anti-Dlx2 antibody, guinea pig serum

Fig Immunohistochemical staining of Dlx2 in mouse embryonic forebrain.
A. 13.5 forebrain cryo-section was immunostained for Dlx2.
B. Enlarged image of A of the septum. Double staining of Dlx2 with Necdin. Arrowheads indicatedouble-stained cells.
The anti-Dlx2 antibody was used at 1/3,000 dilution and anti-Necdin antibody (BioAcademia 74-100)
was at 1/1,000.

Fig 2. Immunohistochemical stainjing of Dlx2 in mouse embryonic forebrain: Comparison with
Nkx2.1, expressed in the similar region.
Sample is E13.5 Sections. The Dlx2 antiserum was used at 1/3,000 (Green) and anti-Nkx2.1 mouse
antibody (Red,Novocastra) at 1/500.
Reference: This antibody has been used in the following publications.
1. Kuwajima T et al. Necdin promotes GABAergic neuron differentiation in cooperation with Dlx
homeodomain proteins. J Neurosci. 2006 May 17;26(20):5383-92. PMID: 16707790 WB, IHC-F(mouse)
2. Long JE et al. Dlx-dependent and -independent regulation of olfactory bulb interneuron
differentiation. J Neurosci. 2007 Mar 21;27(12):3230-43.PMID: 17376983 IHC (mouse). KOValidated
2 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

3. Colasnte G et al. Arx is a direct target of Dlx2 and thereby contributes to the tangential migrationof GABAergic interneurons. J Neurosci. 2008 Oct 15;28(42):10674-86. PMID: 18923043, ChIP(mouse)
4. Hansen DV et al. Non-epithelial stem cells and cortical interneuron production in the humanganglionic eminences. Nat Neurosci. 2013 Nov;16(11):1576-87.IHC-F (human)
5. Wu H et al. Dnmt3a-Dependent Nonpromoter DNA Methylation Facilitates Transcription ofNeurogenic Genes. Science. 2010 Jul 23; 329(5990): 444–448. PMID: 20651149 IHC-F (mouse)
6. de Chevigny A et al. Dynamic expression of the pro-dopaminergic transcription factors Pax6 andDlx2 during postnatal olfactory bulb neurogenesis. Front Cell Neurosci. 2012 Feb 27;6:6. PMID:22371698. IHC (mouse)
7. Kuwajima T. et al. Necdin Promotes Tangential Migration of Neocortical Interneurons from BasalForebrain. J Neurosci. 2010 Mar 10;30(10):3709-14. PMID: 20220004. IHC-F (mouse)
8. López-Juárez A. et al. Thyroid hormone signaling acts as a neurogenic switch by repressing Sox2
in the adult neural stem cell niche. Cell Stem Cell. 2012 May 4;10(5):531-43. PMID: 22560077.
IHC-F (mouse)
9. Wang Y et al. ERK inhibition rescues fate-specification defects of Nf1-deficient neural progenitors
and brain abnormalities. Cell. 2012 Aug 17;150(4):816-30. PMID: 22901811. IHC-P (mouse)
10. Ghanem N. et al. The Rb/E2F pathway modulates neurogenesis through direct regulation of theDlx1/Dlx2 bigene cluster. J Neurosci. 2012 Jun 13;32(24):8219-30. PMID: 22699903.IHC-F(mouse)
11. Gajera CR etal. LRP2 in ependymal cells regulates BMP signaling in the adult neurogenic niche.
J Cell Sci. 2010 Jun 1;123(Pt 11):1922-30. PMID: 20460439. IHC (mouse)
12. Potter GB et al. Generation of Cre-transgenic mice using Dlx1/Dlx2 enhancers and theircharacterization in GABAergic interneurons. Mol Cell Neurosci. 2009 Feb;40(2):167-86. PMID:
19026749. IHC-F (mouse)
13. Bluske KK et al. β-Catenin signaling specifies progenitor cell identity in parallel with Shhsignaling in the developing mammalian thalamus. Development. 2012 Aug;139(15):2692-702.
PMID: 22745311 IHC-F (mouse)
14. Walzlein JH et al. The antitumorigenic response of neural precursors depends on subventricularproliferation and age. Stem Cells. 2008 Nov;26(11):2945-54. PMID: 18757298 IHC (mouse)
15. Rolando C et al. Extracerebellar progenitors grafted to the neurogenic milieu of the postnatal rat
cerebellum adapt to the host environment but fail to acquire cerebellar identities. Eur J
Neurosci. 2010 Apr;31(8):1340-51. PMID: 20384777. IHC-F (rat)
16. Zhong J et al. The Wnt receptor Ryk controls specification of GABAergic neurons versus
oligodendrocytes during telencephalon development. Development. 2011 Feb;138(3):409-19.
PMID: 21205786.IHC-F (mouse)
17. Tepavčević V et al. Inflammation-induced subventricular zone dysfunction leads to olfactory
deficits in a targeted mouse model of multiple sclerosis. J Clin Invest. 2011 Dec;121(12):4722-34.
3 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/PMID: 22056384. IHC-F (mouse)
18. Azim K et al. 3-dimensional examination of the adult mouse subventricular zone reveals lineagespecific microdomains. PLoS One. 2012;7(11):e49087. PMID: 23166605. IHC-F (mouse)
19. Mukhopadhyay A et al. BMP receptor 1A regulates development of hypothalamic circuits criticalfor feeding behavior. J Neurosci. 2012 Nov 28;32(48):17211-24. PMID:23197713. IHC-F (mouse)
20. Liu J et al. Prospective separation and transcriptome analyses of cortical projection neurons andinterneurons based on lineage tracing by Tbr2 (Eomes)-GFP/Dcx-mRFP reporters. Dev
Neurobiol. 2016 Jun;76(6):587-99. PMID: 26248544. IHC (mouse)
21. Azim K et al. Early Decline in Progenitor Diversity in the Marmoset LateralVentricle,Cerebral

Cortex, Volume 23, Issue 4, 1 April 2013, Pages 922–931.
https://doi.org/10.1093/cercor/bhs085. IHC (marmoset)
22. Huang Z et al. Necdin Controls Proliferation and Apoptosis of Embryonic Neural Stem Cells in an
Oxygen Tension-Dependent Manner. J Neurosci. 2013 Jun 19;33(25):10362-73. PMID: 23785149.
IHC-F (mouse)
23. Danjo T et al. Subregional specification of embryonic stem cell-derived ventral
telencephalic tissues by timed and combinatory treatment with extrinsic signals. JNeurosci. 2011 Feb 2;31(5):1919-33.IHC (mouse)
24. Okado H et al. The transcriptional repressor RP58 is crucial for cell-division patterning andneuronal survival in the developing cortex. PMID: 19409883. IHC-F (mouse)
25. Arshad A et al. Extended Production of Cortical Interneurons into the Third Trimester of Human
Gestation.

Cerebral Cortex, Volume 26, Issue 5, 1 May 2016, Pages 2242–2256.

https://doi.org/10.1093/cercor/bhv074. IHC-F (human).
26. Komitova M et al. Hypoxia-induced developmental delays of inhibitory interneurons are reversedby environmental enrichment in the postnatal mouse forebrain. J Neurosci. 2013 Aug
14;33(33):13375-87. PMID: 23946395 IHC-F (mouse)
27. Cocas LA et al.Emx1-lineage progenitors differentially contribute to neural diversity in thestriatum and amygdala. J Neurosci. 2009 Dec 16;29(50):15933-46. PMID: 20016109.IHC-F(mouse)
28. Esumi S et al. Method for single-cell microarray analysis and application to gene-expression
profiling of GABAergic neuron progenitors. Neurosci Res. 2008 Apr;60(4):439-51. 18280602. IF/IC(mouse)
29. Maira M et al. Role for TGF-β superfamily signaling in telencephalic GABAergic neuron
development. J Neurodev Disord. 2010 Mar;2(1):48-60. PMID: 20339443. IHC-F (mouse)
Related products
74-117 Anti-Dlx5 antibody, guinea pig serum

4 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/