GPRC5DGPRC5D is a seven-transmembrane protein and belongs to the orphan receptors. It is highly
expressed on the surface of primary multiple myeloma cells, whereas in normal tissues, it has
minor expression in hair follicles. Studies have shown that 65% of multiple myeloma patients
have a GPRC5D expression threshold of more than 50%, making GPRC5D a potential target
for the treatment of MM. The expression profile of GPRC5D is independently from BCMA in MM
patients, so it can be developed as a single-target or dual-target drug. The research onCHGPRC5D has been focused on bispecific antibodies: CD3×GPRC5D bispecific antibodies(Johnson & Johnson), BCMA×GPRC5D bispecific antibodies (URENCO), and GPRC5D CAR-Ttherapy (Memorial Sloan Kettering Cancer Center).

DIMA Biotech offers many functionally validated GPRC5D proteins and antibodies, which canbe used in different applications, such as immunogens, CAR T-cell development and validation,

Bioactive GPRC5D Proteins

TEBioassays, etc.

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ECD Proteins: Active human GPRC5D recombinant protein is produced
from HEK293 cells which are likely to have the native protein structuresand post-translational modifications.
•The purity >95% as determined by SDS-PAGE•Active binding to anti-GPRC5D antibody in ELISA validation assay
Product Name

Human GPRC5D Protein, mFc Tag

BICat. No.

PME100559

Cat. No. PME100677

PME100560

Mouse GPRC5D Protein, hFc Tag

PME100561

Cynomolgus GPRC5D (1-21) Protein, hFc Tag

PME100066

Human GPRC5D Protein, hFc-His Tag

PME100677

Human GPRC5D Protein, hFc Tag

PME100559PPE-conjugated Human GPRC5D Protein, mFc Tag

DIMAFull Length Nanodisc Protein: Full length active human GPRC5D
recombinant protein is produced from HEK293 cells and purified inNanodisc format.
•The purity >95% as determined by SDS-PAGE•Active binding to anti-GPRC5D antibody in ELISA validation assay

Full Length Exosome Protein: Full length active human GPRC5D
recombinant protein on exosome.
•Active binding to anti-GPRC5D antibody in ELISA validation assay

Cat. No. FLP100011

ELISA Analysis of GPRC5D exosomesCat. No.

Product Name

FLP100011

Human GPRC5D full length protein-synthetic nanodisc

FLP100010

Human GPRC5D full length protein-exosome

Nanoparticle Tracking Analysis of GPRC5D exosomes

TEM image of GPRC5D exosomesCat. No. FLP100010

400-006-0995

info@dimabio.com

Suzhou / Wuhan

www.dimabio.com

GPRC5D Antibodies
Product Name
Anti-GPRC5D antibody(DM60), Rabbit mAb

DME100061

Anti-GPRC5D antibody(DM61), Rabbit mAb

DME100062

Anti-GPRC5D antibody(DM62), Rabbit mAb

DME100089

Anti-GPRC5D antibody(DM89), Rabbit mAb

DME100090

Anti-GPRC5D antibody(DM90), Rabbit mAb

DME100091

Anti-GPRC5D antibody(DM91), Rabbit mAb

DME100060BBiotinylated Anti-GPRC5D antibody(DM60), Rabbit mAb

DME100061B

Biotinylated Anti-GPRC5D antibody(DM61), Rabbit mAb

DME100062B

Biotinylated Anti-GPRC5D antibody(DM62), Rabbit mAb

DME100089B

Biotinylated Anti-GPRC5D antibody(DM89), Rabbit mAb

RecombinantProtein

B Cell LibraryRabbit mAbs

DME100090B

Biotinylated Anti-GPRC5D antibody(DM90), Rabbit mAb

DME100091B

Biotinylated Anti-GPRC5D antibody(DM91), Rabbit mAb

DME100091P

PE-conjugated Anti-GPRC5D antibody(DM91), Rabbit mAb

TE

Flow cytometry analysis with Anti-GPRC5D mAb(Cat. No. BME100072, Talquetamab) on Expi293
cells transfected with human GPRC5D (Blue) orExpi293 transfected with irrelevant protein (Red) .

Cat. No.
DME100060

CHBenchmark Antibody

Featured Data for Cat. No. DME100061

O

Expi 293 cells transfected with irrelevant protein (A) and human
GPRC5D (B) were surface stained with Rabbit anti-GPRC5D mAb(clone: DM61) followed by Alexa 488-conjugated anti-rabbit IgG
secondary antibody.

DIMA

BI

ELISA analysis of Rabbit anti-GPRC5DmAb with purified human GPRC5D proteinAntibody Engineering

Flow cytometry data of serially titrated Rabbit antiGPRC5D mAb (clone: DM61) on H929 cells. The Y-axis
represents the mean fluorescence intensity (MFI) while theX-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti- GPRC5D mAb clones bytitration of different concentration onto H929 cells. The Y-axis
represents the mean fluorescence intensity (MFI) while the Xaxis represents the concentration of IgG used.

Affinities after humanization

Animal survival data for CAR-T therapy
K562-GPRC5D stable cell line

IHC staining of H929 cells A. with rabbit anti-GPRC5D mAb (Cat# DME100061) at
5.9 ug/ml. B. with another anti-GPRC5D mAb (Cat# DME100090) at 8.8 ug/ml.
DME100061 can specifically react with GPRC5D in IHC application.

Internalization assay for ADC development

For more products and services, please visit www.dimabio.com

400-006-0995

info@dimabio.com

Suzhou / Wuhan

www.dimabio.com