PCNA (human), functional
10-15120μg,10-152

PCNA (Proliferating cell nuclear antigen)

100μg

is a homotrimeric protein (261 aa; 29 kDa) known to act

as a co-factor for DNA polymerase , which is responsible for leading strand DNA replication. PCNA
was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis
phase of the cell cycle. Crystal structure data suggests that a PCNA homotrimer ring encircles andslides along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cellcycle control bind to PCNA rather than directly associates with DNA, thus facilitating rapid
processing of DNA. PCNA is a useful marker for DNA synthesis

and some cancers. It is highly

conserved among most amimals.
Applications confirmed:
1. Functional studies on DNA replication, recombination and repair. (Ref 2, 3, 5, 6, 7, 8, 9, 10).
2. Identification of proteins interacting with PCNA by using PCNA –conjugated resin. (Ref 1, 5)
3. Ubiquitination targets (Ref 4, 9, 10).
4 SDS-PAGE (Fig. 1).

5. Western blot (Fig. 2) .

6. Dot blot.

7. ELISA.

Not tested for otherapplications.
Source: Human PCNA was over-expressed in E. coli as a recombinant full-size protein without any tagand highly purified.
Form: 1.0 mg/ml in 25 mM HEPES (pH 7.9), 1 mM EDTA,
0.01% Nonidet P40, 1 mM DTT, 2 ug/ml leupeptin, 0.1 mM PMSF, 75 mM NaCl, 50% glycerol.
Storage: Sent at 4℃ or -20℃. Upon arrival spin-down and store at -20℃ (storage

or at -80℃ for longer)

Purity: Greater than 98% purity as determined by SDS-PAGE (Fig.1).
Data Link: Swiss-Prot
HHHHHHTUTUTUTUTUTU

Q9PTP1UUUUUUTTTTTTTTH TTHTTHTTHTTHTTH

HHHHHHTTTTTTTTUTTUTTUTTUTTU TTU(Zebrafish).

P12004UUUUUUTTTTTTTHTHTHTHTHTH(human),HHHHHHTUTUTUTUTUTU

P04961UUUUUUTTTTTTTTHTTHTTHTTHTTHTTH(rat),

HHHHHHTUTUTUTUTUTU

P17918UUUUUUTTTTTTTT HTTHTTHTTHTTHTTHTTTTT T

TTTTTT(mouse),TTTTTT

References: This product has been used in the following References.
TTTTTT

1. Ohta S. et al (2002) A proteomics approach to identify proliferating cell nuclear antigen(PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex
as a novel PCNA-binding protein. J Biol Chem 277: 40362-40367 PMID:

HTHHHHHTUTUTUTUTUTU

12171929

UUUUUUTTTTTTTTHTTHTTHTTHTTHTH.

2. Iida T. et al (2002) “PCNA clamp facilitates action of DNA cytosine methyltransferase 1 onhemimethylated DNA. Genes Cells 7: 997-1007 PMID:
TTTTTT

12354094

UUUUUUTTTTTTHHHHHH.

TTTTTT

3. Shiomi Y, et al (2004) The reconstituted human Chl12-RFC complex functions as a second PCNA
TTTTTT

loader.
TTTTTT

HHHHHHTUTUTUTUTUTU

HHHHHHTTTTTT

Genes Cells.

TTTTTTHHHHHH

TTTTTT

9:279-90. PMID:

HHHHHHTUTUTUTUTUTU

15066120UUUUUUTTTTTTHHHHHH

TTTTTT.

4.. Watanabe K, et al. (2004) Rad18 guides pol eta to replication stalling sites through
TTTTTTphysical interaction and PCNA monoubiquitination.
TTTTTT

HHHHHHTUTUTUTUTUTU

15359278

BioAcademia,Inc.

UUUUUUTTTTTTHHHHHH

TTTTTT

HHHHHHTTTTTTEMBO J.

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23:3886-96 PMID :.

Tel. 81-6-6877-2335

Fax. 81-6-6877-2336

info@bioacademia.co.jp

http://www.bioacademia.co.jp/en/

TTTTTT

5. Tsurimoto T, et al. (2005) Human Werner helicase interacting protein 1 (WRNIP1) functions as a
TTTTTTnovel modulator for DNA polymerase delta.
TTTTTT

HHHHHHTTTTTT

Genes Cells.

TTTTTTHHHHHH

10:13-22. PMID

HHHHHHTUTUTUTUTUTU

15670210UUUUUUTTTTTTHHHHHH

6. Nishitani H, et al. (2006) Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1
TTTTTTfor proteolysis.

HHHHHHTTTTTTEMBO J.

TTTTTTHHHHHH

25:1126-36. PMID:

HHHHHHTUTUTUTUTUTU

16482215UUUUUUTTTTTTHHHHHH.

7. Shiomi Y, et al. (2007) A second proliferating cell nuclear antigen
TTTTTT

Ctf18-replication factor C, stimulates DNA polymerase eta activity.
PMID:

HHHHHHTUTUTUTUTUTU

17545166UUUUUUTTTTTTHHHHHH

HHHHHHTTTTTT

TTTTTT

loader complex,

J Biol Chem.

TTTTTTHHHHHH

282:20906-14.

TTTTTT.

8. Masuda Y, et al. (2007) Dynamics of human replication factors in the elongation phase of DNA
replication.

HHHHHHTTTTTT

Nucleic Acids Res.

TTTTTTHHHHHH

35:6904-16. PMID:

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17932049

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9. Tomida J, et al. (2008) DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C
complex.

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J Biol Chem.

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283:9071-9. PMID:

HHHHHHTUTUTUTUTUTU

18245774

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10.Tsuji Y, et al. (2008) Recognition of forked and single-stranded DNA structures by human RAD18
complexed with RAD6B protein triggers its recruitment to stalled replication forks.
Cells.

TTTTTTHHHHHH

13:343-54. PMID:

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18363965

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Genes

.

12345755037
PCNA
2520(kDa)
Fig. 1. SDS-PAGE anlysis
of purified PCNA protein.

Fig. 2 Western Bloting of PCNA. Lane 1; Purified PCNA
(3 ng). Lane 2; Purified PCNA (1 ng). Lane 3; Purified
PCNA (0.3 ng). Lane 4; Crude extract of Hela cells (10μg). Lane 5; Crude extract of HeLa cells (2μg) . Primaryantibody is anti-PCNA antibody, BioAcademia # 70-080.

Related product; #70-080 Anti- PCNA antibody, rabbit polyclonal
BioAcademia,Inc.

Tel. 81-6-6877-2335

Fax. 81-6-6877-2336

info@bioacademia.co.jp

http://www.bioacademia.co.jp/en/