DIMA Highlights

Innovations in Therapeutic Antibody Discovery
and Functional Protein Expression

DimAb® Single B mAb Development
1 Expression vector

23Animal immunization

Bioactive protein

5

B cell enrichment
Positive screening

4 PBMC collection

67

Recombinant mAbexpression

Antibody genecloning

8 mAb evaluation(ELISA, FC, IHC etc.)

DimAb® immunized B cell libraries
B7-H3

GPCR5D

CHRM2

CD45
AFP

PD-L1

CD117

Ki-67

Her-2

Prevali㍲ted B Cell Libraries

20 Days

1. High Cloning Eﬃciency: One animal, 10,000+ positive clones

2. High Sequence Diversity: B cells from more than 5 Immunized Animals
3. Fast Cloning Speed: 20 days from B cells to validated IgG sequences

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Lead mAbs For Drug Discovery

5000+ In-stock IgG Sequences
400+ Druggable Targets• Immediate Testing (Pre-clinical Validation Data Packageavailable)• Functional evaluation data on diﬀerent modality
platforms (CAR-T, ADC, BsAb, etc.)• Mammalian Cell Display Based Antibody Engineering
Platform for lead Optimization(Humanization, Aﬃnity Maturation, PTM Risk Removal)• Full Development Solutions for Multi-transmembrane
Targets (Nanodisc, VLP, MNP, Exosome, ECD)

5R7GP

7H1CDL3DL

Lead mAbs For Drug Discovery

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Discovery of ADC Lead Molecules▪ Oﬀ-the-shelf Proteins & Lead mAbs
▪ Prevalidated B Cell Libraries▪ Anti-payload and Anti-linker mAbs

▪ Antibody Internalization Assays▪ Custom ADC Projects

Discovery of ADC Lead Molecules

Hot pre-validated Lead mAbs against ADC Targets
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Discovery of ADC Lead Molecules

Rabbit recombinant mAbs against ADC payloads and linkerTargets

MMAE

Catalog No.

Anti-MMAE antibody(8B4); Rabbit mAbDME101005

Anti-MMAE antibody(9C4); Rabbit mAb

DME101004
DME101006
DME101007
DME101024

Dxd
SN38
CL2A (ADC linker)

Description

DME101003

DME101025
DME101026
DME101027
DME101020
DME101021
DME101022
DME101023

Anti-MMAE antibody(8C4); Rabbit mAb

Anti-MMAE antibody(11B2); Rabbit mAb
Anti-MMAE antibody(11C8); Rabbit mAb
Anti-Dxd antibody(1A1); Rabbit mAb
Anti-Dxd antibody(1A5); Rabbit mAb

Anti-Dxd antibody(1A12); Rabbit mAb
Anti-Dxd antibody(1E6); Rabbit mAb

Anti-SN38 antibody(1G1); Rabbit mAb

Anti-CL2A(ADC linker) antibody(1H6); Rabbit mAb
Anti-CL2A(ADC linker) antibody(1G9); Rabbit mAb

Anti-CL2A(ADC linker) antibody(1H2); Rabbit mAb

IgG labeling reagents for mAb internalization studies
Catalog No.

Product Description

AME100002

DiTagTM pH sensitive IgG labeling reagent plus

AME100001
AME100003

DiTagTM pH sensitive IgG labeling reagent

DiTagTM MMAE IgG labeling reagent

The ﬂuorescent signal from GPRC5D ADC

Cell inhibition detected by CCK8 method. B7H3 ADC

positive cells (K562-GPRC5D stable expression cell(Cat. No. AME100003)

BMK-AME100002 conjugate is only detected in GPRC5Dline), indicating speciﬁc internalization.

is labeled with DiTagTM MMAE IgG labeling reagent

Innovative Products for ADC Drug Discovery04

Discovery of CAR-T Lead Molecules

GPRC5D scFv-hFc binding 8226 cell

The sample marked with a red arrow is the reference CAR (BMK)GPRC5D MM.1S SURVIVAL
group05

Discovery of CAR-T Lead Molecules

DiLibraryTM: Mammalian Cell Display
Based Antibody Engineering Technology1. The design:

2. A natural screening system to ﬁsh out molecules with better
developability:

Structurally and chemically unstable molecules can be cleared out through mammaliancell internal quality control system.

Mammalian Cell Display-Based Antibody Engineering Platform

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DiLibraryTM applications:
Antibody aﬃnity maturation

CDR3 focused mutagenesis

3CDR

CDR

Suﬃcient library diversity

1

CDR2

AI optimized mutagenesis library

Case study:

Aﬃnity maturation for CCR8 therapeutic antibody (WT)

100-fold increase of aﬃnity with only one amino acid mutation at each of LCDR3 and HCDR3.

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Mammalian Cell Display-Based Antibody Engineering Platform

DiLibraryTM applications:
Antibody Humanization

FR precise mutagenesis

Original
GraftedBetter CDR conﬁguration

Human IgG FR germline library

Case study:

Humanization of Anti-BCMA rabbit monoclonal antibodyAnalyte

Ka (1/Ms)

Kd (1/s)

KD (nM)

Rmax (RU)

Hu-BCMA1

3.1023E+4

4.3530E-6

0.14

105.2

Rabbit BCMA
Hu-BCMA2

Hu-BCMA3

Hu-BCMA4

4.3884E+4

2.8604E+4

6.4527E+43.3508+4

1.1750E-5

4.5480E-6

1.0250E-6

1.9060E-6

0.267

0.159

0.015

0.056

65.3

78.9

81.3

92.1

Comparing with parental rabbit IgG, the humanized antibody (Hu-BCMA3) exhibits 18-foldincrease on binding aﬃnity to its target.

Mammalian Cell Display-Based Antibody Engineering Platform08

Custom Nanobody Development

Immunized Alpaca or
Llama (Camelid)

Collect Lymphocytes

VHH Library Generation(4 weeks)

Validate VHH Activity

Express & Purify VHH
Hits(2~3 weeks)

VHH Library Screening &Nanobody Validation (3~4weeks)

Eﬃcient delivery of FACS-validated
nanoantibody sequencesOption for Customer Lock-in: One Alpaca,
One Immunization, Exclusively Yours.

Mammalian Cell Display: Elevating
Antibody Quality and Developability.

One-stop service for humanization
and aﬃnity maturation

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Nanobody Development Platform

Solutions for theFull-length Multi-pass
Transmembrane Proteins
5 Production Platforms

Drug Targets for Cancer Therapy
Function Validated Proteins

VLP

Exosome

MNP

Synthetic Nanodisc

Detergent

Bioactive Proteins For Drug Discovery

10Synthetic Nanodiscs

Multipass Transmembrane Proteins, Right oﬀ the Shelf.
- Full length membrane proteins- High purity validated by PAGE/SEC- High solubility in aqueous solutions- High stability, shipped as lyophilized powder,stable at room temperature- Native membrane environment and remain
biologically active- No detergent, good for cell-based assays- No MSP backbone protein

Human MDR-1 full length protein-synthetic

ELISA analysis using anti-GPRC5D monoclonal

12-pass ABC Transporter, Cat. No. FLP100029

GPRC5D full length protein-synthetic nanodisc,nanodisc on SDS-PAGE

antibody (Cat. No. DME100090) and puriﬁed human
7-pass GPCR, Cat. No. FLP100011

The purity of Human SLC7A11 full length protein-synthetic nanodisc is greater than 90% as determined by SEC-HPLC.
12-pass Cystine/glutamate transporter, Cat. No. FLP100048

11Bioactive Proteins For Drug Discovery

In-Stock Synthetic Nanodiscs forMulti-Pass Transmembrane Proteins
Featured GPCR Proteins

TargetCat. No.

Product name

ADORA2A

FLP100020

Human ADORA2A full length protein-synthetic nanodisc

ADGRE2
C5AR1
CB1

CCR1

CCR3

FLP100090

FLP100086

FLP100023

FLP100094

FLP100075

Human ADGRE2 full length protein-synthetic nanodisc
Human C5AR1 full length protein-synthetic nanodisc
Human CB1 full length protein-synthetic nanodisc

Human CCR1 full length protein-synthetic nanodisc
Human CCR3 full length protein-synthetic nanodisc

CCR4FLP100024

Human CCR4 full length protein-synthetic nanodisc

CCR7

FLP100060

Human CCR7 full length protein-synthetic nanodisc

CCR6

CCR8

CCR9

CXCR1

CXCR2

FLP100059

FLP100037

FLP100061

FLP100091

FLP100066

Human CCR6 full length protein-synthetic nanodisc

Human CCR8 full length protein-synthetic nanodisc

Human CCR9 full length protein-synthetic nanodisc

Human CXCR1 full length protein-synthetic nanodisc
Human CXCR2 full length protein-synthetic nanodisc

CXCR3FLP100053

Human CXCR3 full length protein-synthetic nanodisc

CXCR5FLP100067

Human CXCR5 full length protein-synthetic nanodisc

CXCR4

CXCR7
F2RL1
FSHR

FLP100074

FLP100095

FLP100036

FLP100047

Human CXCR4 full length protein-synthetic nanodisc

Human CXCR7 full length protein-synthetic nanodisc
Human F2RL1 full length protein-synthetic nanodisc
Human FSHR full length protein-synthetic nanodisc

FZD10FLP100052

Human FZD10 full length protein-synthetic nanodisc

GPR75

FLP100031

Human GPR75 full length protein-synthetic nanodisc

GCGR

GPRC5D
HCRTR1
LGR4

FLP100085

FLP100011

FLP100099

FLP100072

Human GCGR full length protein-synthetic nanodisc

Human GPRC5D full length protein-synthetic nanodisc
Human HCRTR1 full length protein-synthetic nanodisc
Human LGR4 full length protein-synthetic nanodisc

LGR5

FLP100073

Human LGR5 full length protein-synthetic nanodisc

SSTR2

FLP100013

Human SSTR2 full length protein-synthetic nanodisc

PTGER4
TSHR

FLP100097

FLP100045

Human PTGER4 full length protein-synthetic nanodisc
Human TSHR full length protein-synthetic nanodisc

Discover Your Protein from Our Comprehensive Nanodisc Collection at the Following
Website: https://www.dimabio.com/synthetic-nanodisc-membrane-protein
Bioactive Proteins For Drug Discovery

12DIMA Highlights
Products

ADC ASSAY REAGENTS

FULL LENGTH
TRANSMEMBRANE PROTEINSECD PROTEINSDIMAB MONOCLONAL ANTIBODIESBIOSIMILAR REFERENCE ANTIBODIES
13Solutions for Your Drug Discovery

DIMA Highlights

Technology Platforms

DiMProTM Membrane Protein Preparation Platform

DimAb® Single B Therapeutic Antibody Discovery

DiLibraryTM Mammalian Cell Display
Based mAb Engineering Platform
DiTagTM Antibody Evaluation
Platform

CLDN6

B7-H3

CCR8

BCMA4-1BB

ROR1

Solutions for Your Drug Discovery

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DIMA Biotechnology
Dedicate on immuno-oncology，Perfect with recombinant mAb development

DIMA Biotechnology+1 (410) 963 4165

info@dimabio. com

Wuhan China

www.dimabio.com

MD USA