400BSIV2.0

BSI Scientific CMOS Cooled Camera

QExFF (%)
1008060402002004006008001000

1100

Wavelength(nm)74fps
CameraLink

40fps
USB3.0Faster Capture

6.5μm
Pixel Size

4.2High Resolution

0.2eDSNU

0.3%
PRNU

1.2 e

-Read Noise

More Accurate
MPResolution

60% QE UV light@ 254 nm

95% QE Visible light@ 550 nm

53% QE Near-infrared@ 850 nmHigh Sensitivity

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A New Breakthrough for New Discoveries!

Since its launch, the Dhyana 400BSI sCMOS camera has been receiving great attention and focus. The image quality of theproduct is fully recognized to be comparable, or even better than its competitors. However, the Tucsen R&D group continues topursue greater levels of excellence. This resulted in an upgraded product, the Dhyana 400BSI [V2.0].
The Dhyana 400BSI [V2.0] achieves a core breakthrough in the transmission speed compared with 400BSI [V1.0], and ahigh-speed data transmission rate of 74fps @ Cameralink and 40fps @ USB3.0 at full resolution. Moreover to meet thedemands for accurate quantification for high-end scientific imaging such as single molecule and super resolution, the 400BSI[V2.0] calibrated DSNU & PRNU, minimized the difference between each pixel and reduced fixed pattern noise. So we are now
able to achieve more accurate quantitative imaging data, providing a guarantee of reliable analysis results. The DSNU & PRNU
calibration is of a great importance of quantitative analysis.
Simultaneously, the Dhyana 400BSI [V2.0] preserves all the essences of Dhyana 400BSI [V1.0], including the latest developedback-illuminated SCMOS sensor, ultra-high quantum efficiency of 95%, microscope-friendly 6.5μm x 6.5μm pixel and 1.2e@ Median ultra-low readout noise.
So, no matter of chasing for brilliant scientific images or accurate images quantitative data, 400BSI [V2.0] is easy toimplement!

DSNU/PRNU calibration, more accurate quantitative analysis
To improve the overall performance of the camera, the Dhyana 400BSI (V2.0) was calibrated in DSNU (dark signal nonuniformity) and PRNU (photo response non-uniformity) characteristically. After calibration, the DSNU value reduced from0.3e- to 0.2e-, the PRNU value reduced from 1.6% to 0.3%. Thus the new upgraded camera has a more sophisticatedcapabilities, making it more suitable for quantitative analysis applications.

BeforeAfter

Dark Signal Non-Uniformity (DSNU) Optimization

BeforeAfter

Photo Response Non-Uniformity (PRNU) Optimization-15°C lower temperature to reduce the impact of noise
Heat transfer

The dark current is closely related to the operating temperature. Whenthe camera is at a low and stable temperature, the generation of darkcurrent will greatly reduce, thereby it reduces the influence of noise on
the imaging and brings benefits to SNR for long exposure low-lightapplications.

Wide spectral response range, high sensitivity
QExFF (%)

With its ultra-low noise, the advantage of the quantum efficiency in

100

95%

Dhyana 400BSI (V2.0) is very significant. This is a huge breakthroughfor scien-tific applications, not only in the visible region, but also in theBack-illuminated
Front-illuminated

80

65%

60
40

82%

40%
19%

20

ultraviolet and near-infrared.
Quantum Efficiency Comparison

7%0200400600

Wavelength(nm)

800

1000

1100

74fps @ CameraLink, 40fps @ USB3.0, faster data capture
In addition to the signal to noise ratio advantage, the Dhyana
400BSI (V2.0) has enhanced transmission speed, on one
hand though the new CameraLink interface to meet theneeds of high-end imaging research for higher frame rates,on the other hand through hardware improvements that
increase the USB 3.0 throughput. These have achieved theultimate transfer rate of 74fps with CameraLink and 40fps
with USB3.0 at 4.2 MP full resolution.

ColumnRow

USB3.0CameraLink

2048

2040

40.4fps

74.0fps

2048

1024

79.9fps

147.9fps

2048

512

158.8fps

293.9fps

2048

256

317.6fps

582.8fps

2048

128

629.2fps

1147.9fps

2048

64

1242.6fps

2227.8fps

Third-party applications
Third-party applications supported by Dhyana 400BSI (V2.0) have also been greatly expanded, including Micromanager,Labview, Matlab, etc., to provide more application support and help.

Customer applications

Possibility

0.4
Dhyana 400BSI
82%QE sCMOS Camera

0.3

400BSI
400BSI

82%QEsCMOS
82%QEsCMOS

The high SNR can effectively improve the intensity of single-0.20.1

molecule fluorescence emission. The statistical results of the0Dhyana 400BSISingle molecule localization

082%QE sCMOS Camera

24681210

Localization(nm)localization accuracy of the fluorescent sphere shows that theComparison

accurancy of localization with 400BSI is twice as that of the third

Source: Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology

generation 82% QE sCMOS camera.

Normalized intensity

Super-resolution imaging
1The lower the FWHM, the higher the resolution. In STORM super0.5resolution imaging, the capturing spatial resolution of 400BSI

00603090120

reaches to 40 nm, while those of the third generation of 82% QEDistance(nm)Dhyana 400BSI

Dhyana 400BSI

82%QE sCMOS Camera

82%QE sCMOS Camera

ComparisonsCMOS can only achieve 47 nm resolution, so the spatial resoluitonof 400BSI with STORM super resolution microscope carries aSource: Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology

superiority of 7nm.

Neuron fluorescence imaging
Camera: Dhyana400BSI
相机：Dhyana
400BSI
Lens:20XNikon（NA0.75）
20X Nikon (NA0.75)
镜头:Fluorescent labeling: GFP neuronal labeling荧光标记：GFP神经元标记
Excitation light: 488nm
激发光源：488nm
ROI: 50μum
m xx50μm
ROI：5050um
Exposure time:ms100 ms
曝光时间：100Source: Zhejiang University School of Medicine
图像来源：浙江大学医学院

Camera: Dhyana 400BSI
Microscope: Fluorescence microscope
Lens: 100X TIRF dedicated oil mirror (NA1.49)
Excitation light: 561 nm
ROI: 55μm x 43μm
Exposure time: 170 ms
Source: College of Optical Science andEngineering, Zhejiang UniversityWith the increasing of the exposure time, luminescent fluorophores
produce phototoxicity to the cells. Compared with other cameras,theexposure time of 400BSI is shorter, which can protect cell samples
from light damage better.

TIRF wide field imaging
In the TIRF applications, the light signal of the samples is very weak,
but 400BSI with the ultra-high SNR camera is able to capture thepractical and good quanlity of images effectively with rather short
exposure time, resulting in a faster and fully widefield imaging.

SIM cytoskeleton imaging
Camera: Dhyana 400BSI
Microscope: Fluorescence microscope
Excitation light: 488nm,fluorescent: 525nmExposure time: 20 ms
fastest frame rate: > 50fps
Source:Suzhou Institute of Biomedical Engineeringand Technology Chinese Academy of Sciences

SIM imaging requires the cameras to capture as sharp as possible
pictures with as low as possible the exposure time, along withothers same shooting conditions, the Dhyana 400BSI [V2.0] has asignificant signal-to-noise ratio advantage, resulting in better
images quality than other cameras.

Technical Specifications

Applications

Model

Dhyana 400BSI (V2.0)· Super-resolution microscopy

Sensor size1.2"· Real-time confocal microscopy

Sensor modelG2020 BSI (Backside-illuminated sCMOS)· Gene sequencing

Color/mono

Monochrome· Live-cell imaging

Quantum efficency

60%@254nm, 95%@550nm, 53%@850nm

Effective resolution

2048(H) x 2040(V)Pixel size

6.5μm x 6.5μm

Effective area

13.3mm x 13.3mm· FRET

Full well capacity

30,000e-· TIRF

74fps @4.2MP @CameraLink· DIC

40fps @4.2MP @USB3.0

Read-out noise

1.2e- (Median); 1.8e- (RMS)

Shutter typeRolling Shutter

Exposure

Manual / Auto, Time: 6.6μs-10s

DSNU

0.2e -PRNU

0.3%Cooling

Forced air (Ambient at +20℃): -15℃

Dark current

0.15 e-/pixel/s (-15 ℃)

Dynamic range

88dB

Binning

2x2 / 4x4

Sub-array

Available

External trigger modeStandard / Synchronous / Global trigger

Trigger delay function

0-10s(1μs steps)

External trigger routing

SMA

Trigger output

3 programable timing output( Exposure / Global / readout signal )Digital interface

USB3.0 / CameraLink

SDK

SupportBit depth

16bit

Lens mountC-mount

Power supply

12V / 8A

Power consumption

55W

Camera size

85mm x 85mm x 125mm· Astronomy observationDimensions
Front Side

Side Face8512585

Frame rate

· Single molecule detection

4-M4

Parameter settings

White balance, Exposure, Contrast, Gamma,
DPC, Saturation, Flat Fielding

PC software

Mosaic / LabVIEW / Matlab / Micromanager

Compatible system

Windows / Linux / Mac

Operating environment

Temperature: 0-60℃ Humidity: 10%-85%

106

Reverse SideDC 12VSWITCHCAMERALINKTRIGGERUSB 3.0

Tucsen Photonics Co., Ltd.
Address: 6F NO.1 building Caimao Zone, 756# Qi’an Road,
Gaishan Town, Cangshan Area, Fuzhou, Fujian, P. R. CHINA
Tel: + 86-591-88194580
Website: www.tucsen.com
Email: support@tucsen.com
Europe/North America OEM/ODM Support:
Address: 1273 Vine St, Denver, CO 80206, USA.
Tel: +1-520-203-2643
E-mail: mikeblake@tucsen.com