Anti-HCV Core protein antibody, mouse monoclonal (H6-29) (biotin)
Product code65-053

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

A part of the core region (nucleotides 369-704, amino acids 13-124) of HCV
genotype 1b expressed in E. coli (the nucleotide sequence is shown in ref.3)

Isotype

Mouse IgG2aκ

Reactivity

Specific to human HCV core antigen of genotype 1b.
Not tested in other genotypes

Special notesConjugation: biotin[biotin] / [IgG] = 6.6

Application

1. Western blotting
2. Immunohistochemistry
3. Immunofluorescence staining
4. ELISA

BackgroundHepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense
single-stranded RNA virus in the family Flaviviridae and the principal cause ofparenteral non-A, non-B hepatitis. The virus genome consists of a single open
reading frame of approximately 9,4 kb which encodes a single polyprotein ofabout 3,010 amino acids (1, 2, 3). The polyprotein is processed by host cell andviral proteases into four structural proteins (core, envelope1 and 2, and p7) andsix non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viralreplication. HCV core protein is not only a component of nucleocapsid but also
has multiple functions and is a pathogenic factor for hepatitis. It also
participates in some cellular processes, including transcriptional regulation andcellular transduction. HCV core antigen is used as diagnostic marker for HCV
infection.

Data Link

Swiss-Prot HCV protein

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-053 Anti-HCV Core protein antibody, mouse monoclonal (H6-29) (biotin)

Fig.1 Western blotting of HCV core protein.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA andwere subjected to Western blotting using this antibody. The core protein is detected as a 22-kDa band.

Fig.2 Detection of HCV core protein by immune-fluorescence antibody staining.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA.
After incubation for 48 hr, the cells were fixed with acetone and HCV core protein was detected byindirect immunofluorescence staining using this antibody.

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Fig.3 Immunohistochemical detection of HCV core protein.
Tissue section from a patient with chronic hepatitis C was immunostained to reveal cells expressing
HCV core antigen, which are scattered in the lobules (indirect immunohistochemical method, counterstained with Mayer’s hematoxylin).
References: This antibody (unconjugated) has been used in ref.4 and 5.
1. Brass V, Moradpour D, Blum HE. Molecular Virology of Hepatitis C Virus (HCV): 2006 Update.

Int J Med Sci 2006; 3:29-34. PMID: 16614739
2. Kato, N. et al. (1990) “Molecular cloning of the human hepatitis C virus genome from Japanesepatients with non-A, non-B hepatitis.” Proc. Natl. Acad. Sci. USA 87, 9524-9528 PMID: 2175903
3. Takamizawa, A. et al. (1991) “Structure and organization of the hepatitis C virus genome isolated
from human carriers.” J.Virol.65, 1105-1113 PMID: 1847440
4. Manabe, S. et al. (1994) “Production of nonstructural proteins of hepatitis C virus requires aputative viral protease encoded by N3.” Virology 198, 636-644 PMID: 8291245
5. Hiramatsu, N. et al. (1992) “Immunohistochemical detection of hepatitis C virus-infected
hepatocytes in chronic liver disease with monoclonal antibodies to core, envelope and NS3 regionsof the hepatitis C virus genome.” Hepatology, 16, 306-311 PMID: 1379209
Related products
65-052 Anti-HCV core protein antibody, mouse monoclonal (H6-29)
65-054 Anti-HCV core protein antibody, mouse monoclonal (H6-29) (FITC)

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/