Anti-SUMO1 antibody, rat monoclonal (4D12)
Product code

70-653Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenPurified recombinant GST-fused human SUMO1 (full length)

IsotypeRat IgG 2aκ

Reactivity

Specific to human, simian, mouse and rat SUMO1.
Other species have not been tested.

Special notes

N/A

Application

1. Western blotting (1/1,000 dilution)
2. Immunofluorescence staining (1/100 dilution)
3. Immunohistochemistry, frozen section (1/100 dilution)
4. ELISA(assay dependent)

Other applications have not been tested.
Background

SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteinsthat are covalently attached to and detached from other proteins in cells tomodify their function. Unlike ubiquitination, which targets proteins fordegradation, SUMO modification plays a critical role in a number of cellularfunctions including nucleocytoplasmic transport, gene expression, cell cycle andformation of subnuclear structures such as promyelocytic leukemia (PML)bodies. There are three confirmed SUMO isoforms in human; SUMO1, SUMO2and SUMO3. SUMO2 /3 show a high degree of similarity to each other and aredistinct from SUMO-1. Individual SUMO family members are all targeted todifferent proteins with diverse biological functions. SUMO-1 is conjugated toRanGAP, PML, p53 and I B-

to regulate nuclear trafficking, formation ofsubnuclear structures, regulation of transcriptional activity and protein
stability. SUMO1 is encoded as a 101 aa protein and first Met and C-terminal
4 aa are removed from the preprotein.
Data LinkSwiss-Prot P63165 (human)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 70-653 Anti-SUMO1 antibody, rat monoclonal (4D12)

Fig.1. Detection of SUMO-1 by Western blotting with the antibody 4D12.
An 80 kDa single and other multiple bands were observed in HeLa total cell extract.
The 80 kDa band would be SUMO-RanGAP.
Anti-SUMO-1 antibody 4D12 was used at 1 μg/ml.

Fig.2. Immunofluorescence staining of SUMO-1 with the antibody 4D12 in the mouse primary culture
neurons.
Left: Stained with anti-SUMO-1 antibody 4D12 at 10μg/ml.
Light: DNA was stained with Hoechst

2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig.2. SUMO-1 colocalizes with SUMO-2/3 as revealed by indirect immunofluorescence staining of C33A cells (human cervix carcinoma).
Left: SUMO-1 was stained with anti-SUMO-1 antibody (4D12) at 10 μg/ml
Middle: SUMO-2/3 was stained with anti-SUMO-2/3 antibody (3H12).
Right: Merged image

Fig.4 Fluorescence immunocytochemistry for endogenous SUMO1 expression.
HEK293A cells were fixed, permeabilized, and stained with SUMO1-FITC (1: 50) antibody andHoechst 33342. Scale bar, 50 mm.
References: This antibody was used in the following publications.
1. Uchimura Y et al “Involvement of SUMO modification in MBD1- and MCAF1-mediatedheterochromatin formation.” J Biol Chem 281: 23180-23190 (2006) PMID: 16757475
2. Saitoh N et al “In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear
rim and PML bodies.” Exp Cell Res 312: 1418-1430 (2006) PMID: 16688858

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/