Anti-GENA (germ cell-specific antigen) antibody, rat monoclonal (TRA98)
Product code

73-003Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Cell lysate of adult mouse testis. Rat hybridoma clone TRA98 was establishedby Dr. Tanaka H and Prof. Nishimune Y at Osaka University

IsotypeRat IgG2aκ

Reactivity

Mouse

Special notes

N/A

Application

1. Western blotting (1/1,000- 1/5,000)
2. Immunoprecipitation (1/200)
3. Immunofluorescence staining (1/400)
4. Immunohistochemical staining (1/200-1/500)
5. Flow Cytometry

BackgroundA monoclonal antibody TRA98 recognizes a mouse testicular germ cell-specificantigen (1). In adult tissues, bands of 60-100 kDa proteins are detected only inthe testis by Western blotting analysis with TRA98 (Fig. 1). The signals areobserved in male and female embryos after embryonic day 12.5. The signal inmale is detected during development of germ cells and also after birth, but thesignal in female disappears by 5 days after birth. The antigen is localized onlyin the nuclear fraction of testicular germ cells and this antibody can be used for
immunohistochemical staining of testicular germ cells (Ref.1, 2).

Data Link
Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 73-003 Anti-GENA (germ cell-specific antigen) antibody, rat monoclonal ( TRA98)

Fig.1. Western blot analysis with TRA98 of various mouse tissues.
The antibody was used at 1 µg/ml The tissue homogenate samples were applied at 100 µg protein
per lane. Note that TRA98 is detected only in testis lysate.

Fig.2. Immunohistochemical staining of a 10-day-old testis with germ cell-specific antibody, TRA98.
Sections of 10-day-old mouse testis were fixed with 4% paraformaldehyde and embedded in paraffin.
After antigen retrieval by boiling for 10 min in 10 mM citrate buffer (pH 6), they were reacted withthe anti-TRA98 antibody (Green) at 1/400 dilution and anti-GATA4 antibody (Red). As the 2nd
antibody, Donky anti-rat Alexa Fluor488 and Donkey anti-rabbit Alexa Fluor594 were used. DNA
was stained with DAPI (Blue) in the above figure.
Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA98 stains
reproductive cells.
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Fig.3. Immunohistochemical staining of a 7-day-old testis with germ cell-specific antibody, TRA98.
Frozen sections were reacted with the antibody and the antibody was detected by the avidin-biotinperoxidase complex method with hydrogen peroxide and diaminobenzidine.
Scale bar; 50 μm

Fig.4 Colocalization of germ cell specific marker, Tra98, with IKAP at PO during spermatogenesis
as shown by imunohistological staining. Image from . PLoS Genet. 2013 May;9(5):e1003516 authoredby Lin FJ et al.
Related products:
73-001 Anti-SLA (haploid sperm cell-specific antigen) antibody, rat monoclonal (TRA54)
References: This product has been used in the following publications.
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6.Kang HS et al. Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of
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Cysts in

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germ cells and Sertoli cells. Dev Biol. 2013 Aug 15; 380 (2): 145-56. PMID:23727514. IHC-P(mouse)21.Lin FJ et al. Ikbkap/Elp1 deficiency causes male infertility by disrupting meiotic progression. PLoS
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22102605. IHC (mouse)
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immunohistochemistry. Methods Mol Biol. 2011;763:223-36. PMID: 21874455. IHC-P (mouse)
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Andrologia. 2011 Jun;43(3):180-8. PMID:21486398. IHC-P (mouse)
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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/Biol. 2009 Jan 1;325(1):238-48. PMID:19014927. IHC-F (mouse)
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64.Kasai S et al. Haploinsufficiency of Bcl-x leads to male-specific defects in fetal germ cells:
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77.Toyoda-OhnoHetgerm cell development in

al..Members of
fetal

mouse

the ErbB receptor tyrosine kinases are involved ingonads.

Dev

Biol. 1999

Nov

15;215(2):399-

406.PMID:10545246. IHC-P (mouse)
78.Tanaka H et al . A germ cell-specific nuclear antigen recognized by a monoclonal antibody raised
against mouse testicular germ cells. Int J Androl 20: 361-366 (1997) PMID: 9568529 WB (mouse)
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