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Fluorescence spectroscopy, also known
as fluorometry or spectrofluorometry, isa type of electromagnetic spectroscopy,
which analyzes fluorescence from a sample. It involves using a beam of light that
excites the electrons in molecules of certain compounds and causes them to emit
light; typically, but not necessarily, visible
light. It is a useful technique in many
biological (chlorophyll and carotenoid),biochemical (fluorescence diagnosis ofmalignancies) and environmental applications. For most fluorescence applications
the amount of fluorescence energy emitted is only 3% of the amount of excitation
light energy. Fluorescence light has a lower
energy (higher wavelength) than the excitation energy and is usually scattered light.
This means it emits energy in all directions.
For optimal performance assuming thetime acquisition window is not limited, Avantes recommends our AvaSpecULS2048LTEC spectrometer for thisapplication, since it can support long integration times often exceeding 5 seconds.
When higher-speed acquisition is required,Avantes recommends the AvaSpecULS2048XL back-thinned CCD spectrometer. For maximal sensitivity thetop model of the SensLine, AvaSpecHS1024x122-USB2 spectrometer is recommended.
When configuring the measurement setup,preventing excitation light from entering
the spectrometer is an important issue.
Possible methods to accomplish this,where one does not exclude the other,include:Make use of an AvaLight-LED lightsource which typically has a narrow
bandwidth enabling the limitation ofexcitation to shorter wavelengths thatare not part of the emission spectrum
Use a broadband light source such as theAvaLight-HAL for high output in combination with an (interference) band-passor low-pass filter.
Make sure the optical path for excitation
light and fluorescence are perpendicular.
This means the excitation light will notenter the receiving fiber (use the CUVUV/VIS-FL or the CUV-DA)
Use the fluorescence decay time to separate excitation energy from the integration time start pulse. Use a pulsed lightsource to accomplish this (pulsed laser orAvaLight-XE Xenon flash)You created this PDF from an application that is not licensed to print to novaPDF printer (http://www.novapdf.com)

In spectroscopy fluorescence is one of themore challenging setups, due to the low
fluorescent emission (about 3% of the excitation energy). The AvaSpec-2048L gives
the highest sensitivity and the AvaLightLED series provides excitation at therequested wavelength.

Typical applications:Dyes identification
Fluorescent lamps
Diagnosis of malignancies
Fluorescent labeling... and many more
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www.phototechnica.co.jp〒336-0017 埼玉県さいたま市南区南浦和 1-2-17

TEL:048-871-0067

FAX:048-871-0068

e-mail:voc@phototechnica.co.jp

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