The Promoter Activities of Reporter Constructs
HeLa cells

HepG2 cells
70.0
60.0

100.0
80.0
60.0
40.0

40.0
30.0
20.0
10.00.00.0

Posi.
Nega.
PPP2
R4AS
NS

20.0

250.0

Hep3B cells

200.0

150.0

100.0

Posi.: pGL3 Control Vector(SV40 promoter)
Nega.: Empty Vector

0.0

AS
NSID
PP
2P1
R15A

50.0

Posi.
Nega.
PP
P2
R4

Relative Light Units (RLU)

50.0

PP ID
P1 2
R15A

Relative Light Units (RLU)

120.0

Posi.
Nega.
PPP2
R4ASNSID
PP2P1R1
5A

Relative Light Units (RLU)

140.0

PPP2R4: pGL4-phPPP2R4 (RDB#7294)
ASNS: pGL4-phASNS(RDB#7305)
ID2:pGL4-phID2 (RDB#7306)
PPP1R15A: pGL4-phPPP1R15A (RDB#7307)
2009, DNA Bank, RIKEN BioResource Center[ GENETYX : Nucleotide Sequence Homology Data ]Date : 2010.01.25
1st Nucleotide Sequence
File Name: Reference Seq.gnu

Sequence Size

: 5488

2nd Nucleotide Sequence
File Name: RDB7307F.fastaSequence Size: 636

Unit Size to Compare = 1
Pick up Location= 1[72.562% / 605 bp]

INT/OPT.Score : <1'179/ 1020 >GG CCTAACTGGC CGGTACCTGA GC-TCGCTAG** * ***** ***** * * * ***1" CATTATAAAG ACAGCCGATC AAACAANCGG CTTAACT-GC GATCGCCTAA CCATAGCTCC
32' CCTCGAGGAT CAGAGTGAGA CGCCTACTCA AAAAATAAAN TAGGCTGGGT GCA-GTAG-C*** * ***** ********* . * ***** * * **** *60" AACCACAGTA AACATCGAGA GCACCATCAC AAAAATAATT TTGATCGGGT GAACGTAGAC
90' TCACGCCTAT AAT-CCT-AA CACTTTGGGA AGCCNGAGGC AGG-CAGACT GCCTGAG-CT*** ** ** *** *** ** ********** ** *.

* * *** **** * ******* **120" ACACACC-AT AATACCTAAA CACTTTGGGA AGACCAGGAC AGGACAGATT GCCTGAGACT
146' CAGGAGTTCG AG-AACAGCC TGGG-CAACN ATGG-CGAAA C-ACTGTCTC T-ACT-AAAA********** ** ******* **** *** . **** ***** * ******** * *** ****
179" CAGGAGTTCG AGAAACAGCC TGGGACAAAC ATGGACGAAA CAACTGTCTC TAACTAAAAA
200' ATACAAAAAA TTAGCTGAGT GTGGNTAGTG CGTG-CCTGT AATCCCAG-C TACTCGGAGG**************** **** ***** **** ***** ******** * **********

239" ATACAAAAAT TAGACTGAGT GTGG-TAGTG CGTGACCTGT AATCCCAGAC TACTCGGAGG

2010 DNA Bank, RIKEN BioResource Center258' -CTGAAGAGG GAGAATCGNC TTGAACCCGG AAGATGGAGG TTGCAGTGAG -CCGAGAT-********* ********.

* ******* ********** ********** *******

298" ACTGAAGAGG GAGAATCGCT TGAAACCCGG AAGATGGAGG TTGCAGTGAG ACCGAGATAC314' CACG-CCATT GCANCTCCAG -CCTCGGTGA C-AGAG-CGA G-ACTCCATC TCAAAAAAAA*** ***** ********** ********* * **** *** * ******** **********358" AACGACCATT GCANCTCCAG ACCTCGGTGA CAAGAGACGA GAACTCCATC TCAAAAAAAA369' ATAAAAAANA TAAAAAATAA CAAA------ --TAAATAAA TAGG--CCTG GAGTGGTGGC********.

* ******* *********** **** **** **********418" ATAAAAAAAT AACAAAATAA TAAATAACCN AATAAATAAA TAGGACCCTG GAGTGGTGGC419' TC-ACG--CC TGNCAATCCC AG--CACTTT -GGGA--GGC CAA--GGCA- GGCAGAACAC** *** ** **. **** ******* ***** * **** ******* * **478" TCAACGACCC TGCAATCCCC AGCCAACTTT GGGGAGGGAC CAAGGGACAG GGCAGGAAAC
468' CTGAGGTCAG GNAGTTCGAG ACCAGCC-TA GCAAACATGG TGAAATCCCG TCTCAGAAAA* ** *.

*** * * *** ** ** * ******* ***538" AACCTG-AAG GTTCAGGGAG ATAAACCGAA ACAGACCTAG CCAAA---CA TGGGTG-AAA527' ATANAATAAT GAATAAATAA ATAAATAAAT AGGCCGGGCG CGGTGACTCA CGCCNTGTAA** . ** * *** ** **..***** **593" ATCCCGT-CT CAGAAAAAAA TTANNTAAAT TGGGAAATTA TCTTT

1st Nucleotide Sequence
File Name: Reference Seq.gnu

Sequence Size

: 5488

2nd Nucleotide Sequence
File Name: RDB7307R.fasta (Complementary)

Sequence Size

: 725

Unit Size to Compare = 1
Pick up Location= 1

2010 DNA Bank, RIKEN BioResource Center[84.311% / 733 bp]

INT/OPT.Score : <436/ 1987 >

601' GAGGCCAAGG CGGGAGGATC CCCTGACGTC GNGGAGTTCC AGACCAGCCT GACCAACATG* *1"

CCGTT661' GAGAAACTAC GTCTCTACTA AANCAAATAC AAAATTAGCC GGGCGTGGCG GCACATGCCT***** *** . * ** **. ** ******** * *** *** ** ****

6" CAGAAAATAA ATANGGA-TA AATAAATAAA TAAATAGGCC GTGCGCGG-T ACTCACGCCT721' GCAATCCCAG CACNTTTGGG AGGCCAAGGC GGGAGGA-TC CCCTGACGTC GGGA-GTTCC* .******* *** ****** ****** *** ******* ** ********** *** ** **64" G-NATCCCAG CAC-TTTGGG AGGCCAGGGC GGGAGGATTC CCCTGACGTC TGGAGGTGCC
779' AGACCANGCC TGACCAACA- TGGAGAAACT ACGTCTCTAC TAAACAAATA CAAAATTANG****** *** ******************* ********** ********** ***.****.*

122" AGACCA-GCC TGACCAACAT TGGAGAAACT ACGTCTCTAC TAAACAAATA CAANATTAGG
838' CCGGGCGTGG CGGCACATGC CTGCAATCCC AGATACTCGG GATGCTGAGN GCAGGAGAAT*** ****** ********** ********** ********** *******************181" CCGTGCGTGG CGGCACATGC CTGCAATCCC AGATACTCGG GATGCTGAG- GCAGGAGAAT
898' C-GCTTGAAC CCCGAA-GGC GGAGGTTGCG GTGAGCCGAG ATNCGCCCC- TTTGCATTCC* ******** ****** *** ********** ********** **. ***************

240" CTGCTTGAAC CCCGAAGGGC GGAGGTTGCG GTGAGCCGAG ATCTGCCCCT TTTGCATTCC
955' AGCC-TGAGC AACAAGAGCA AAACTCCGCC TCAAANATAA CTAACTAAAT AAATAAATAA**** ***** ********** ********** ***** **** ********** **********
300" AGCCTTGAGC AACAAGAGCA AAACTCCGCC TCAAA-ATAA CTAACTAAAT AAATAAATAA1014' ATTGGGGTGG GAAGGCATTG ACCCTGNAGC TGAGAGAGAA CTGGCTCGCT GTTGAAGCAG********** ********** ****** *** ********** ********** **********
359" ATTGGGGTGG GAAGGCATTG ACCCTG-AGC TGAGAGAGAA CTGGCTCGCT GTTGAAGCAG
1074' GGCCACGCAT TTGATTGNAC AGTTCGTTTG TTGGAGGGGC GTGGTCACGC TCGGAAACTC********** ******* ** ********** ********** ********** **********418" GGCCACGCAT TTGATTG-AC AGTTCGTTTG TTGGAGGGGC GTGGTCACGC TCGGAAACTC2010 DNA Bank, RIKEN BioResource Center

1134' CGCCGTGANC GTTGCAAAAG CTGGAATCTC CGCGAGAAGT CCTGTCTTAC TTCCACTTCN******** * ********** ********** ********** ********** *********477" CGCCGTGA-C GTTGCAAAAG CTGGAATCTC CGCGAGAAGT CCTGTCTTAC TTCCACTTC1194' CCACCCTTCG GGTTGCGGTC TCGAAACCCC GCCTCTCTTC GTGACGTCAG NCACGCCGGG********** ********** ********** *************** ** ** **535" CCACCCTTCG GGTTGCGGTC TCGAAACCCC GCCTCTCTT- GTGTAGTTCG -CA-GCGGGT
1254' CGCGGTAGG- CTATAAAAG- CCTAGTGGCC ATTGTGATCA AGATCTGGCC TCGGCGGCCA*****. ************** ** * ******** * ** * ** **592" CGCGGNTGGT CTATAAAAGT CCTGGTAGAC CATGTGTATC GGAT-GGTCC TGGTGGTCCG
1312' AGCTTGGCAA -TCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA* **** * *** * * * **** * ** * *** * ****651" ACGTGTTCAA NTGCGCCTAT GGGGTTTTAG TAACTACCGA GGGT----AG ACCTTTAAAC
1371' AG-GGCCCAG CGCCATTCTA CCCACTCGAA GACGGGACCG CCGGCGAGCA GCTGCACAAA** ******707" AGAGGCCCAA ACTNTAATT

2010 DNA Bank, RIKEN BioResource Center