Anti-DNA polymerase κ (human), mouse monoclonal (# 13)
Product code70-073

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Recombinant human DNA polymerase kappa (1-560aa) with His6-tag at Cterminal (BioAcademia10-105)

Isotype

Mouse IgG1κ

Reactivity

human and rat. Expected to react with mouse from the sequence.

Special notes

N/A

Application

1.Western blotting (1 µg/mL)
2.Immunoprecipitation (1~5 µg/mL)
3.ELISA (assay dependent)

Background

Mammalian DNA polymerase

κ, a member of the UmuC/DinB nucleotidyltransferase superfamily, has been implicated in spontaneous mutagenesis (1).
Human DNA polymerase κ copies undamaged DNA with average single-basesubstitution and deletion error rates of 7 x 10PP-3PP and 2 x 10PP-3PP, respectively.
These error rates are high when compared to those of most other DNA polymerases(2).

DNA polymerase κ has important role in the mutagenic bypass of certain typesof DNA lesions (3).Expression of DNA polymerase kappa is much higher in testis
than in other tissues and the shorter transcript due to alternative splicing is majorform.

Data Link

UniProKB Q9UBT6 (POLK_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-073 Anti-DNA polymerase κ (human), mouse monoclonal (# 13)

Fig.1. Western blot of human testis tissue lysate
10 µg of human testis tissue lysate (221 HT-401, Funakoshi) was run on SDS-PAGE (10% gel).
Anti-DNA polymerase kappa antibody was used at 1µg/ml.
Second antibody (goat anti-mouse IgG antibody, HRP-conjugated, ab205719) was used at 1/5,000dilution.
The upper band corresponds to the full-size product of 99 kDa and the main band corresponding to~50 kDa is likely the product of testis specific alternative splicing

Fig.2 Western blot of recombinant DNA polymerase kappa (BioAcademia 10-105)
1 µg of recombinant human DNA polymerase kappa (65 kda)was run on SDS-PAGE (10 % gel).
Anti-DNA polymerase kappa antibody was used at 1µg/ml.
Second antibody (goat anti-rmouse IgG antibody, HRP-conjugated, ab205719) was used at 1/5,000
dilution.

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Fig.3 Immunoprecipitation of recombinant DNA polymerase kappa (BioAcademia10-105)
10 µg of recombinant DNA polymerase kappa was immune-precipitated with 10µg of anti-DNApolymerase kappa antibody and the precipitate was immune-blotted with anti-DNA polymerase
kappa antibody.
Lane 1 : recombinant DNA polymerase kappa
Lane 2 : mockFig.4. Titration of protein of polymerase kappa by indirect ELISA using monoclonal antibody
The indicated amounts of recombinant DNA polymerase kappa was coated onto the wells of the ELISA
plate. After blocking with 1% BSA, the 2 µg/ml of monoclonal antibody was added to the each well.
HRP-conjugate goat anti-mouse IgG (100μl, x10,000 dilution) was added. As substrate, TMBZ wasused. Optical density (OD) measured at 450nm.
Reference

This product has been used for the following reference.

1. Ohashi E et al. (2000) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, theproduct of the human DINB1 gene. J Biol Chem 275: 39678-39684 (2000) PMID: 11006276
2. Ohashi E et al. (2000) Error-prone bypass of certain DNA lesions by the human DNA polymerase
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kappa” Genes Dev 14: 1589-1594 (2000) PMID:10887153 .
3. Valasco-Miguel S et al. (2003) Constitutive and regulated expression of the mouseDinb(Polκ)geneencoding DNA polymerase kappa. DNA Repair 2:91-106. PMID: 12509270 (Alternative splicing)
4. Jałoszyński P. et al. (2005) Error-prone and inefficient replication across 8-hydroxyguanine (8oxoguanine) in human and mouse ras gene fragments by DNA polymerase kappa. Genes Cells.
10:543-50. PMID: 15938713
Related Products:10-105 DNA polymerase kappa (human)

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