LC Technical Note

Simultaneous LC/MS/MS Analysis of Pesticides Used inGolf Courses

LT107
GL Sciences Inc.

In 1990, Japanese Ministry of the Environment announced a “Interim Guidance Guidelines on Prevention of Water
Contamination by Pesticides for Golf Courses”. Furthermore, the last revision 100929001 announced by theEnvironment Management Bureau, Ministry of Environment on Sep 29, 2010, states to measure 44 pesticides byLC/MS/MS.
Both quantifier and qualifier ions must be measured besides fenitrothion.
In this technical note, we introduce results of simultaneous analysis of 43 pesticides using 3200 QTRAP LC/MS/MS
System (AB SCIEX) and InertSustain C18 HPLC column.
XIC of +MRM (63 pairs): 223.000/126.000 amu Expected RT: 6.6 ID: Acetamiprid 1 from Sample 18 (100_sMRM) of TEST.wiff (Turbo Spray)

Max. 1.4e5 cps.

6.0e5

Intensity, cps

5.0e5

Positive

4.0e5

3.0e5
2.0e5
1.0e5

0.0
24
1220

6
148161018

22

2426

2830Time, min
XIC of -MRM (24 pairs): 328.000/243.000 amu Expected RT: 13.1 ID: Iprodione 1 from Sample 19 (100_Neg_sMRM) of TEST.wiff (Turbo Spray)

Max. 539.5 cps.

4.0e5

Intensity, cps

3.5e5
3.0e5
2.5e5

Negative

2.0e5
1.5e5
1.0e5
5.0e4
0.0246810

1214Conditions
Column: InertSustain C18 3 μm, 150 × 2.1 mm I.D.
Eluent: A) 5 mM Ammonium acetateB) CH3OH
A/B = 80/20 - 13 min - 10/90 -10 min hold, v/v* Reequilibration for next analysis: 7 min
Flow Rate : 0.25 mL/min
Col. Temp. : 40 ℃
Detection : LC/MS/MS (3200 QTRAP: ESI,MRM)
Inj. Vol.
: 5 μL
Data Supply： Mr. Tateda in AB SCIEX, Japan

161820Time, min2224262830

GL Sciences LC Technical Note

MRM (Q1/Q3)
Components
QualifierQuantifier(ESI,Positive)
ion
ion
R.T.
Thiamethoxam
292/132
292/211
4.7 Imidacloprid
256/175
256/209
5.8 Clothianidin
250/132
250/169
6.0 Acetamiprid
223/126
225/128
6.6 Ethoxysulfuron
399/261399/218
9.0 Simazine
202/104
202/68
9.6 Metalaxyl
280/220280/192
10.9 Azoxystrobin404/372404/329
11.8 Siduron
233/137
233/9411.9 Flutolanil
324/242324/262 12.2
Propyzamide
256/190258/192
12.4
Mepronil
270/119
270/9112.4
Isoprothiolane
291/231291/189
12.5
Cafenstrole
351/100
351/7212.5
Cumyluron
303/185
303/125 12.5
Cyproconazole
292/70292/125
12.5
Simeconazole
294/73294/70
12.7
Tetraconazol
372/159372/7012.7
Triflumizole metabolite
295/215295/176
12.8
Tebuconazole
308/70310/7013.4
Propiconazole
342/159344/161
13.6
Diazinon
305/169
305/9714.0
Butamifos
333/180
333/9614.0
Pencycuron
329/125
329/89 14.0
Difenoconazole
406/251408/253 14.1
Isoxathion 314/105 314/97 14.2
Terbucarb 278/222 278/166 14.2
Triflumizole 346/278 348/280 14.4
Oxaziclomefone 376/190 376/161 14.7
Pyributicarb
331/181331/108 15.5
Pendimethalin282/212282/194
15.8

MRM (Q1/Q3)
Components
QualifierQuantifier(ESI,Negative)
ion
ion
R.T.
Flazasulfuron
406/251
406/154
6.7 Harosulfuron-methyl433/252433/788.0 Cafenstrole metabolite
250/186
250/67
8.7 Triclopyr
254/196
256/198
8.9 Mecoprop
213/141
215/143
9.3 Cyclosulfamuron 420/265
420/7810.8 Boscalid
341/112343/11212.0 Thifluzamide
527/125
527/166
12.9 Iprodione328/243
330/245
13.1 Tebufenozide
351/149
351/105 13.1
Bensulide396/213396/111
13.1
Dithiopyr
400/352
400/304
14.8(Conc. of each compound: 100 μg/L)

GL Sciences LC Technical Note

＜Compounds to be detected LC/MS/MS (ESI, Positive) - 1＞

Acetamiprid

Clothianidin

Difenoconazole

Imidaclopri
dAzoxystrobin

CumyluronEthoxysulfuron

Isoprothiolane

Butamifos

Cyproconazole

Isoxathion

Cafenstrole： An isomer exist

DiazinonFlutolanil

MepronilGL Sciences LC Technical Note

＜Compounds to be detected by LC/MS/MS (ESI, Positive) - 2＞

Acetamiprid

Clothianidin

DifenoconazoleImidaclopri
dAzoxystrobin

CumyluronEthoxysulfuron

Isoprothiolane

Butamifos

Cyproconazole

Isoxathion

Cafenstrole： An isomer exist

DiazinonFlutolanil

MepronilGL Sciences LC Technical Note

＜Compounds to be detected by LC/MS/MS (ESI, Negative)＞
Flazasulfuron

Triclopyr

Boscalid

Tebufenozide

Harosulfuron-methyl

Mecoprop

Thifluzamide

Bensulide

Cafenstrole metabolite

Cyclosulfamuron

Iprodione

Dithiopyr

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly. We reserve theright to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences (ShangHai) Ltd.

GL Sciences, Inc. USA

22-1 Nishishinjuku 6-Chome
Shinjuku-ku, Tokyo,163-1130, Japan
Phone: +81-3-5323-6620
Fax: +81-3-5323-6621
Email: world@gls.co.jp
Web: www.glsciences.com

De Sleutel 95652 AS Eindhoven
The Netherlands
Phone: +31 (0)40 254 95 31
Email: info@glsciences.eu
Web: www.glsciences.eu

Tower B, Room 2003,
Far East International Plaza,NO,317 Xianxia Road,
Changning District.
Shanghai, China P.C. 200032
Phone: +86 (0)21-6278-2272
Email: contact@glsciences.com.cn
Web: www.glsciences.com.cn

4733 Torrance Blvd. Suite 255
Torrance, CA 90503
Phone: 310-265-4424
Fax:310-265-4425
Email: info@glsciencesinc.com
Web: www.glsciencesinc.com

International Distributors
Visit our Website at:https://www.glsciences.com/company/distributor.html