Anti-HEV (Hepatitis E Virus) Capsid antibody, mouse monoclonal (161)
Product code65-090Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Recombinant truncated capsid protein (amino acids 112–608) ofHEV (genotype 3)

Isotype

Mouse IgG1

Reactivity

Capsid protein of HEVSpecial notes

Epitope: P domain (amino acids 457 to 608) of HEV capsid protein.

Application

1. Western blot (1/500~1/1,000 )2. Immunofluorescence staining (1/500)
3. ELISA (assay dependent)
Other applications have not been tested.

BackgroundHepatitis E virus (HEV) is a single-strand positive-sense RNA virus in thefamily Hepeviridae. The disease caused by HEV is an important public healthproblem in developing countries. A molecular phylogenetic analysis classifies
HEV into four major genotypes (genotype 1-4). The genome HEV consists ofabout 7200 bases and contains three discontinuous and partially overlapping
open reading frames (ORFs). ORF1 encodes a methyltransferase, protease,helicase and replicase; ORF2 encodes the capsid protein and ORF3 encodes aprotein of undefined function. The viral capsid protein induces neutralizingantibodies, and contains three subdomains, S (aa112-319), M (aa 320-456) andP (aa 457-608). Recombinant HEV-VLP is composed of approximately 53 kDa,
smaller capsid protein subunit.

Data Link

UniProKB Q6J8F7 (CAPSD_HEVMG), genotype 3

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-090 Anti-HEV (Hepatitis E Virus) Capsid antibody, mouse monoclonal (161)

(kDa)

AB

100 ー
75 ー← 53 KDa

50 ー37 ー
25 ー
20 ー
15 ー

1 2

12

Fig.1. Identification of protein in HEV by SDS-PAGE (A) and Western blotting (B) using monoclonal
antibody
Lane 1: Recombinant HEV- VLP (2.0mg/ml).
Lane 2; Recombinant HEV-VLP (0.5mg/ml)
The proteins were applied to SDS-PAGE and stained with Coomassie Brilliant Blue (CBB). In
Western blotting, the monoclonal antibody was used at 1/500 dilution. A 53 kDa band was identified
as HEV-VLP capsid protein.

Fig.2. Detection of HEV protein infected in PLC/PRF/5 cells by immunofluorescence staining(a) Infected and cultured cells on a slide glass. (b) Smear preparation after treatment with trypsin.
The infected cells were fixed in cold acetone. The MAb was used at 1/500 dilution. As the second
antibody,FITC-conjugated rabbit anti-mouse IgG was used at 1/4,000 dilution The nucleus (DNA)was stained with DAPI. HEV multiplies in cytoplasm.

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Fig.3. Titration of protein of HEV by indirect ELISA using monoclonal antibody

The indicated amounts of recombinant

HEV-VLP was coated onto the wells of the ELISA plate.

After blocking with 5% skim milk, monoclonal antibody at the 1/5000 dilution was added to the eachwell. HRP-conjugate goat anti-mouse IgG (100μl, x4000 dilution) was added. As substrate,orthophenylenediamine was used. Optical density (OD) measured at 490nm.
Reference: This antibody was described and used in the following publication.
1. Yamashita T et al.Biological and immunological characteristics of hepatitis E virus-like particles
based on the crystal structure. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12986-91 PMID:
19620712 IP, ELISA

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