Pertussis Toxin from Bordetella pertussis01-50325 µg

Storage: Shipped with dery-ice and store at -80℃.
Applications:
1. Studies on the signal transduction of the trimeric GTP binding proteins.
2. Therapeutic role in treating a number of diseases including hypertention, viral and autoimumune
inhibitions.
3. Used for producing model mice for multiple sclerosis
State:

50~500 µg/ml depending on lot in 10% glycerol 10 mM phoshate buffer (pH 7.4), 137 mM NaCl

Background: Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterrapertussis.

PT catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine

nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction involving
the G proteins.

PT consists of one moplecule of each S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12kDa) and two molecule of S4 (12 kDa).

This product was highly purified (>90% pure) from Bordetella

pertussis strain Tohama by the method of Skelton & Wong1). Cytotoxicity of the PT was confirmed bymorphological alteration of CHO cells after treatment with 0.1 ng/ml of PT (see the Figure below).
Data Link: UniProtKB P04977 (TOX1_BORPE), P04978 (TOX2_BORPE), P04979 (TOX3_BORPE),
P0A3R5 (TOX4_BORPE), P04981 (TOX5_BORPE)
References:
1. Skelton SK and Wong KH “Simple, efficient purification of filamentous
hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic
and affinity interaction.” J. Clin Microbiol 28:1062-1065 (1990) PMID:2351723
2. Alouf JE & Popoff MR (Ed.) The comprehensive Sourcebook of Bacterial
Protein Toxins 3rd Ed. Academic Press (2006)
Fig.1

Assay: 0.1 ng/ml of PT in the culture medium of CHO cells at 37℃ for 17 hr )PT（ー），×10PT（0.1ng/ml ），×20

Fig.2 SDS-PAGE of PT(silver staining)
Left: Control culture without PT

Right: CHO cells treated with PT at 0.1 ng /ml* Research use only, not for human use.
BioAcademia,Inc.

Tel. 81-6-6877-2335

Related Product; 64-030 anti-Pertussis Toxin antibody

Fax. 81-6-6877-2336

info@bioacademia.co.jp

http://www.bioacademia.co.jp/en/Material Safety Data Sheet
Pertussis Toxin
Harzardous Ingradient
Pertussis toxin highly purified (>90% pure) from cultured medium of Bordetella pertussis
strain Tohama
50~500 µg/ml depending on lot, in 50% glycerol, 10 mM phosphate buffer pH 7.3, 75 mM
NaCl.

Health Hazard Data
The LD50 of pertussis toxin in mice is 15~21 μg/kg by intraperitoneal injection.

Emergency Procedure
If the toxin is accidentally swallowed, induce vomiting. Since the toxin is unstable inacidic conditions, it will be degraded in stomach.
If skin pricking occurs accidentally, bleed and perform vigorous flushing of the area withlarge amounts of water. If injection occurs, seek a physician’s advice immediately.
Hyperimmune globulin is the only antidote.
However, persons immunized with Pertussis (whooping cough) vaccine will unlikely have long term
adversary effects.

Handling
It should be handled carefully by persons with expertise in knowledge and techniques forthe safe handling of bacterial toxins. Avoid mouth pipetting. Wear protective gloves on
handling the toxin. Avoid contact with open wounds. Wash thoroughly any area of the bodythat makes contact with the toxin. It is recommended that persons who handle the toxin are
immunized by pertussis vaccine.
.
Inactivation
The toxin can be inactivated by boiling for 30 min.

BioAcademia,Inc.

Tel. 81-6-6877-2335

Fax. 81-6-6877-2336

info@bioacademia.co.jp

http://www.bioacademia.co.jp/en/