GC Technical NoteGT004

Analysis of Cherry Blossoms Fragrance - using MonoTrap

GL Sciences Inc.

MonoTrap is a hybrid novel adsorbent that combines a large surface area and the properties of silica gel,activated carbon, and ODS. Due to the large surface area of porous silica and the adsorption effect causedby the inclusion of activated carbon, a high collection efficiency is obtained. Therefore, high-sensitivity
analysis can be performed in a short time. In the case of flowers or the like, the fragrance component
changes over time (ripening). To analyze this change in a simple method, we used MonoTrap DCC18 (withactivated carbon) to perform simple enrichment analyses of the fragrance components of cherry flowers
and cherry flowers ripened by prolonged warming to track the changes in the fragrance components.
Using MonoTrap DCC18, we identified Coumarine and others that were not identified in live flowers byripening. This Coumarine is an aroma produced by salting and ripening cherry flowers. It is actually used toscent Japanese food cherry rice cake.
ProtocolAging
Cherry BlossomsSample
A piece of cherryblossoms branch

A piece of cherry
blossoms flower

Passive Sampling
MonoTrap : DCC18 5 pcs
at 60 ℃, 24 hours (Aging)

MonoTrap :DCC18 5 pcs 24 hours

Solvent Extraction/
Concentration
Diethyl ether 1000 µL, Ultrasonication
5 minsConcentration by N2 purge to a few µL③MT Extract Cup with Vial
(Start UP-Kit)

GC/MS
GC Condition
Sｙstem： SHIMADZU GC-2010, GCMS-QP2010

Column：InertCap Pure-WAX (Cat.No. 1010-68142)
0.25 mm I.D.×30 m df=0.25 μm

Column Temp ：40 ℃ (5min)→4 ℃/min→180 ℃→20 ℃/min→250 ℃ (5 min)Carrier Gas：He 120 kPa
Injection：Split 1:10, 1 µL

Detection：MS Scan (m/z: 40-350)

Ultra inert WAX column InertCap Pure-WAX is highly recommended to analyze
aromatic compounds together with MonoTrap

GL Sciences GC Technical NoteAging

134 5621Clorius

2

2,6-Dimethyl-3,7-octadiene-2,6-diol

3p-Anysaldehyde

4

3,5-Dimethoxybenzaldehyde

568-Hydroxylinalool
Coumarine

MonoTrap

Convenient MonoTrap Start-UP-KIT
Description①②③④⑤⑥⑦⑧⑨Sample

Red・・・Identification result ofstandards

MT Holder
MT StandMT Extract Cup withVial (20 mL)
Clean Pin Hole Septum withvial (40 mL)
200μ L Glass Insert (Flat Bottom)
MonoTrap DCC18
MonoTrap RCC18
MonoTrap DSC18
MonoTrap RSC18Qty.
51554020
202020①②④③⑤

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly. We reserve theright to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences (ShangHai) Ltd.

GL Sciences, Inc. USA

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