LC Technical Note

Reduction of Solvent Consumption in HPLC (2020 #3)–Column Selection

LT185

GL Sciences Inc.

GL Sciences has been suggesting reduction of solvent consumption in HPLC for more than 15 years. This technical note
describes how to reduce analysis times and solvent consumption by selection of C18 columns. The example here
includes the use of low retentivity C18 columns and changing the column dimensions, which do not require
adjustment of the isocratic conditions.
(K. Suzuki)

Low Retentivity C18 Columns

Analysis times and solvent consumption can be reduced only by changing the packing material without any change inconditions or column dimensions. Below is an example of column change from a high retentivity C18 column toInertSustainSwift C18, the retentivity of which is low because of a low carbon load (9%) and a small specific surface
area (200 m2/g). If the peaks are separated enough, this technique is recommended as a simple way to reduce theanalysis time and solvent consumption.

Analysis of Carotenoids in Seawead
Column： Competitor C18 ( 5 μm, 4.6 mm x 150 mm)
Carbon load > 10 % , Specific surface area > 300 m2/gConditions
System：GL7700Column: C18 column AMobile phase: A) CH3CNB) H2O
A/B = 75/25, v/v: 1.0 mL/minmAU
46(5 μm, 4.6 mm x 150 mm)

12Flow rate

0

Interfering compound

020Time (min)4060

Change to a low retentivity column (same dimensions)
Column: InertSustainSwift C18 (5 μm, 4.6 mm x 150 mm)
Carbon load: 9%, Specific surface area: 200 m2/g

Column Temp. : 40 ℃
Detection: VIS 500 nm

Inj. Vol.

: 20 μL

Analyte: Seaweed extract1．Fucoxanthin

Note: Please refer to LT030 for the sample pretreatment.

Comparison of retentivity
among InertSustain andother brands’ C18 columns
InertSustainSwift C18
InertSustainSwift
C18

6InertSustainSwift
InertSustainSwift C8C8InertSustain C18
C18
InertSustain

mAU
41

InertsilODS-3ODS-3InertsilAnalysis time more than halved!

市販ODS-A
C18
column A

2Interfering compound

C18
column B
市販ODS-B
C18
column C
市販ODS-C

0C18
column D
市販ODS-D

020Time (min)4060

Retentivity for alkylbenzenes

GL Sciences LC Technical Note
Further Reduction of Analysis Time is Possible!
This technique does not require any change in the analytical conditions such as the mobile phase composition. In theexample below, a 3-fold reduction of the analysis time is achieved by simply changing the particle diameter andcolumn dimensions. This reduction of the analysis time also decreases the solvent consumption.
3-fold Reduction of Analysis Time and Solvent Consumption!
Column : InertSustainSwift C18

Column : InertSustainSwift C18

3 µm, 3.0 mm x 100 mm

5 µm, 4.6 mm x 150 mm

Flow rate : 1.0 mL/min

Flow rate : 1.0 mL/min

Pressure: 10.2 MPa

88Pressure: 5.0 MPa

mAU
4 610022mAU
4 612010Time (min)030

08 10Time (min)2030

Other Features of InertSustainSwift C18• For small and mid-sized (up to Mw ≈ 20,000) molecules!

• Fast equilibration!
Comparison of Equilibration
InertSustainSwift C18(Pore Size: 200Å)

Analysis of 3 oligonucleic acids
Competitor C18(Pore Size: 120Å)

InertSustainSwift C18(Pore Size: 200Å)

Competitor C18(Pore Size: 130Å)

Time (min)Time (min)Equilibration timeA) LongB) Very short(1/6 of A)

Almost stableC) Short(1/3 of A)

StableUnstable

Please contact us for more detailed technical information.

Please refer to LB514 for the analytical conditions.

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

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