Anti- Streptolysin O (SLO) antibody, rabbit serum
Product code

64-001Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.09% sodium azide

PurityRabbit antiserum

ImmunogenHis6-tagged full-size recombinant SLO of S. dysgalactiae subsp. equismilis
H46A (group C hemolytic Streptococci) expressed and highly purified from E.

coliIsotype

Rabbit IgG

Reactivity

Streptolysin O of Group A, C and G origins

Special notes*Most of the anti-Streptolysin O polyclonal antibodies (ASLO) sold in thecurrent market were obtained by immunizing with SLO purified from theculture supernatant of hemolytic Streptococci. But the SLO preparations werecontaminated with other secreted proteins such as NADase. This product ishighly specific to SLO because of the purity of immunogen.

Application

1. Western blotting (x2,000~10,000 dilution). Dot blotting
2. Neutralization of hemolysis by Streptolysin O (SLO)
3. Immunoprecipitation
4. ELISA

Background

Streptolysin O (SLO) is a membrane-damaging extracellular toxin producedby hemolytic streptococci.

In the hemolytic streptococci infection, theantibodies to various proteins secreted from the bacteria such as toxins
increase. For the diagnostic confirmation of SLO production by the infection,
the measurement of the antibody, ASLO (Anti-Streptolysin O), is most widelyused.

Data Link

UniProtKB Q54114 (TACY_STREQ)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

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Data Images: 64-001 Anti Streptolysin O (SLO) antibody, rabbit serum

Fig. 1 Western blot analysis of culture supernatant from GAS strain Sa and GCS strain H46A withthe anti-SLO antibody.
Lane 1; Medium only
Lane 2*; Culture supernatant of GAS strain Sa
Lane 3**; Culture supernatant of GCS strain H46A
The antibody was used at 1/2,000 dilution* Most of SLO in culture medium of GAS strain Sa is proteolytically degraded, accounting for low
activity level of SLO in the culture of this strain. Condensed sample is analyzed in Fig. 2.
** The two faint bands below SLO band are partially degraded SLO.

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Fig.2. Western blot analysis of concentrated culture supernatant of GAS strain Sa with the anti-SLOantibody. Culture supernatant was approximately 1,000~2,000 fold concentrated from culture
medium by ammonium fractionation and heat-precipitation.
Lane 1; Concentrated culture of GSA strain Sa
Lane 2; Concentrated medium only
The SLO of GSA strains is degraded by an exoprotease called streptococcal pyrogenic exotoxin B andboth the concentration and size of SLO in culture medium is decreased from native one.
References: This antibody has been described in Ref.1 and used in the following publications.
1.Kimoto H. et al.: Expression of recombinant streptolysin O and specific antibody production.

J.

Mol. Microbiol. Biotechnol.,10: 64-68 (2005). PMID:16491027 WB
2. Minami M et al “Clindamycin-induced CovS-mediated regulation of the production of virulentexoproteins streptolysin O, NAD glycolhydrolase, and streptokinase in Streptococcus pyogenes.”
Antimicrob Agents Chemother. 2010 Jan;54(1):98-102 PMID: 19805566 WB
3. Potez S. et al. Tailored protection against plasmalemmal injury by annexins with different Ca2+sensitivities. J Biol Chem. 2011 May 20;286(20):17982-91. PMID:21454475 Immuno
electronmicroscopy
4. Baruch M et al. An extracellular bacterial pathogen modulates host metabolism to regulate its own
sensing and proliferation. Cell. 2014 Jan 16;156(1-2):97-108. PMID: 24439371 WB

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