Ministry of Education, Culture, Sports, Science and Technology (MEXT)
Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer Project

Issue No. 10
Mar c h , 2 0 1 2

Overview of FNCA Biofertilizer Project 2011
Tadashi Yokoyama, Tokyo University of Agriculture and Technology (TUAT)
In order to promote environmental friendly
sustainable agriculture in Asia, FNCA Biofertilizer
Project aims to develop biofertilizers withradiation sterilization technology, using beneficalmicroorgamisms, which increase the yields of crops
while reducing the environmental burden ofexcessive use of chemical fertilizers.
At the first phase of this project from 2001 to 2006,
FNCA Biofertilize Manual was published, which
gives information and experiences of biofertilizer
use in Asian countries, their effectiveness, efficient
production processes, storage and application ondifferent crops, as an important outcome.
At the second phase from 2007 to 2012, a main
objective is developing multifunctional biofertilizer
having both function of plant growth promoting
and resistance against plant pathogens. And othertwo objectives are improvement of inoculants byradiation-based microbial mutation breeding inorder to keep high quality inoculant under tropicalcondition, and dissemination of radiation
sterilization method of carrier using 60CO to
improve quality of carrier for biofertilizers.
In this newsletter, we introduce the activities of
the FNCA Biofertilizer Project in 2011.
At first, we would like to introduce the FNCA 2011
Workshop on Bifertilizer Project. It was held on
September 27th - 30th in Ulaanbaatar, Mongolia
and a total of 22 participants from 8 countries
participated.
At the workshop, summaries of research activities
in this phase were reported by the participating
countries. In addition, a discussion was held on
specific activities to be conducted in the next phasestarting in fiscal 2012, and agreements were madeon activities such as evaluation of survival abilityof microorganisms for biofertilizers, publication of
the “FNCA Guideline for Biofertilizer Quality

Analysis” on these microorganisms and quality
control in 2012, and development of a radiation
breeding technique for developing multifunctional
biofertilizers. Furhter, exriments of synergy effectbetween biofertilizer and irradiated oligochitosan
was planned as a new research activity.
It is also confirmed that irradiation sterilization ofcarriers has a better result compared to autoclave,in view of viability of the inoculant in carrier,production cost of biofertilizer in big volume forcommercial purposes. Private companies in
Malaysia and Indonesia have already used theirradiation of inoculant carrier for commercial
purpose, however all Project Leaders should makeefforts to enhance the use of radiation sterilizationof carriers in collaboration with nuclear research
center in their own countries and more precise costcomparison of radiation sterilization andautoclaving should be studied.
The participants visited the Mongolian State
University of Agriculture (MSUA) to check thetypes of crops, seasons for seeding and harvesting,fertilizers used and contents of studies conductedin Mongolia, and inspect the soil chemical analysis
laboratory, microbiology laboratory, etc. in theuniversity.

Participants of the Workshop in Mongolia

1Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer ProjectCountry News

Biofertilizer Activities in Bangladesh
Md. Saidul Islam, Bangladesh Atomic Energy Commission (BAEC)In Bangladesh, following organizations are involved

The Bangladesh Institute of Nuclear Agriculture hasorganizations involved in biofertilizer activities:

developed peat based rhizobial inoculants for six pulse■ Bangladesh Institute of Nuclear Agriculture

crops and one for dhaincha cultivation, which are cheap■ Bangladesh Agricultural University

and easy to handle. All these inoculants are environment■ National Institute of Biotechnology

friendly and can be used as substitutes of nitrogen■ Bangladesh Agricultural Research Institutefertilizer. The country's legume production can be■ Bangabandhu Sheikh Mujibur Rahman Agricultural

increased substantially by application of the inoculants,

University

which will be helpful in removing the malnutrition
problem of the country. Moreover, it also improves thesoil health.

Awareness program for end users of biofertilizer
Bangladesh Agricultural University has alreadyresearch and ancillary facilities. Environmental

released Rhizobium biofertilizer for nut, soybean

biotechnology division of the Institute is involved inand lentil. Research is on going on cyanobacterial

the development of rice biofertilizer. Soil and ricebiofertilizer for rice cultivation and effect ofroot samples have been collected and from different

Biofertilizer and Plant Growth Regulators on growth

localities of the country to isolate bacterial strains.

of summer mungbean.

The strains were identified and nitrogen fixing

National Institute of Biotechnology

is a newly

potentiality has been studied. Rice seeds are

established Institute under the Ministry of Science

inoculated by the selected strains to observe theirand Technology, Govt. of Bangladesh with adequate

effect on germination and plant growth.
2Colony of Azospirillum strain M-1 on Different Media

RC

BMS

N-plusRice seeds of 1 day after inoculation

Rice seed of 6 days after inoculation

Bangladesh Agricultural Research Institute is the

Bangabandhu Sheikh Mujibur Rahman Agriculturallargest multi-crop research institute conducting

University is working on Rhizobium as a crop

research on Biofertilizer (Rhizobiun inoculum) for

enhancer and biofertilizer for increased cereal

increased production of pulses. The production of

production. Rhizobacteria as bioenhancer andgrasspea, lentil, mungbean, gardenpea, chickpea,

biofertilizer for growth and yield of banana (Musasoybean increases by 4-59% due to the use of BARI

spp. cv. ‘Berangan’)developed Rhizobium inoculum.

Table: Recommended biofertilizers for use in Bangladesh
Crop

Institutes

Selected biofertilizer

LentilAgriculture University, BINA

DUM-160, BINA-LT-18

Gram

BINA

BINA-CP-2

CowpeaBINA

BINA-COP-7

Dhaincha

BINA

BINA-DC-9

Borboti

BINA

BINA-MB-1

Mugbean

BINA

BINA-MB-1

Nut

Agriculture University, BINA

BAU-700, BINA-GN-2

SoybeanAgriculture University, BINA

BAU-1007, BINA-SB-4

3

Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer Project

FNCA Biofertilizer Research Activity in 2011
Fan Bingquan, Chinese Academy of Agricultural Sciences (CAAS)
In

2011,

high

effective

phosphate-solubilizing

2. The experiment results of phosphate-solubilizing

biofertilizer were developed and field experiments

biofertilizer in field conditions

were conducted.

Eight

1. Suitability of phosphate-solubilizing strains with

developed based on above research results. Field

soil types and crops.

experiments to evaluate the effects of the biofertilizers

The suitability of phosphate-solubilizing strains with

were conducted in Hebei Province and Heilongjiang

various soils and different crops were studied in pot

Province.

experiment under greenhouse conditions. Phosphate-

2.1. effects of 4 kind of biofertilizers on soybean

solubilizing strain P30-1and P31 are suitable foryields in Heilongjiang

Sierozem and corn; strain P24-3 and P40-1 are

Compound fertilizer at a rate of 25kg/666.7m2 assuitable for Vertisol -winter wheatsustem; strain P24-5,

N-P2O5-K2O 15-15-15 was applied to all treatments.

P36-1 and P31 are suitable for Paddy soil and canola；

Four kinds of phosphate-solubilizing biofertilizer werestrain P30-1and P36 are suitable for Salinized Chao

tested and each was applied at a rate of 15 kg/666.7m2.

soil and canola; strain P2-1A and P21% for ALBISOL

The results showed that biofertilzer can increase

and corn；strain P2-1A, P41-3Aand P36 for Chao soil

soybean yield compared to control (CK). Application

and wheat; strain P21 and P36 for Chao soil and cornof No.1, No.5, No.6, and No.7 biofertilizer havesystem；atrain P2-1A, P30-1and P41-3A are suitable

increased soybean yield by 16.0%, 9.8%, 6.3% andfor Drab soil corn; strain P30-1, 41-3A, P36 and

2.4 % in Longmen Farm and by 14.9%, 10.6%, 6.1%

P2-1A are suitable for Chernozemic soil and cornand 13.7% in Keshan County in Heilongjiang

system; strain P36, P10-7and P30-1 are suitable for

Province (table 1). Biofertilizer No.1 had got theBlack soil and soybean system；strain P36, P21 andhighest yield of soybean in both sites.

phosphate-solubilizing

P10-7 are suitable for Black soil and corn condition.
Table 1. Effects of 5 biofertilizers on soybean yields in black soils

4biofertilizers

were2.2 Effects of phosphate-solubilizing biofertilizers

Chemical fertilizer at a rate of N 12.5, P2O53.3 andon corn yields in field experiment in Cangzhou

K2O 6kg/666.7m2 was applied to all treatments. Five

City, Hebei Province

kind of phosphate-solubilizing biofertilizers as no.1,

Cmpound fertilizer as N-P2O5-K2O 17-6-22 wasno.4, no.5, no.7, and no.8 were tested and each of2

applied at a rate of 25 kg/666.7m to all treatments.

them was applied at a rate of 25 kg/666.7m2.

Five kind of phosphate-solubilizing biofertilizers were

The results showed that no.4 biofertilizer had got thetested and each biofertilizer was applied at a rate of 25

highest2

yield(average

695.84kg/666.7m2)

that

kg/666.7m .

increased by 34.21% compared to control; application

Application of No.4 biofertilizer increased corn yieldof no.8 biofetilizre had got a significant higher yieldby 11.45% and No.7 biofertilizer increased by 10.14%(average 655.31 kg/666.7m2), it increased by 26.39%

over control in Chao soil (table 2).

above control (table 3). Other biofetilizer treatments

3.3 Effects of phosphate-solubilizing biofertilizers

had got a obvious increase of corn yield compared toon corn yields in field experiment in Shijiazhuang

control.

City, Hebei Province
Table 2. Effects of biofertilizers on corn yields in Cangzhou city (kg/666.7m2)

Table 3. Effects of biofertilizers on corn yields in Shijiazhuang City (kg/666.7m2)

Demonstration & field experiment of phosphate-solubilizing biofertilizers

5

Biofertilizer ProductForum for Nuclear Cooperation in Asia (FNCA) Biofertilizer ProjectEvaluation of Bio-Organic Fertilizer to Substitute Partly an
Inorganic Fertilizer for Sustainable Rice Cultivation
Dr. Iswandi Anas and Dr. Muchlis M. Bakrie
National Nuclear Energy Agency and Bogor Agricultural University, Indonesia
Background
More than 73% of agricultural soil in Indonesia

To make it attractive to the farmers, granule form ofincluding rice field have been degraded as indicatedbio-organic fertilizer is a good choice and the dosage

by low organic matter content of the soil. Although

is kept at the rate of hundreds kg per ha.

the dosage of inorganic NPK fertilizers was increased,
there was no significant increase of yield was

Aim of the study was to study the ability ofobserved. Irrational application of pesticides with abio-organic fertilizer to reduce the dosage of inorganic

high dosage caused serious environmental pollution.

NPK fertilizer without reducing yieldIn the last few years, the price of raw materials forphosphorous and potassium fertilizers increased

Treatments:

tremendously. The use of good quality of organic

A1 = Conventional rice cultivation; A2 SRI rice

fertilizers is the best alternative to cope all problems

cultivation; P0 = Without fertilizer; P1 = 100%

mentioned above. Organic fertilizers can be improved

Inorganic Fertilizer (Urea 200kg; SP-36 75 kg; KCltheir quality by using beneficial soil microbes such as

50kg; P2 = 75% Inorganic Fertilizer + BIOST 200kg;

Azotobacter, Azospirillum and phosphate solubilizing

P3 = 50% Inorganic Fertilizer + BIOST 200kg; P4 =fungus. It is known as bio-organic fertilizer. However,

50% Inorganic Fertilizer

farmers are reluctant to use a big amount of organic

BIOST

fertilizer.

phosphate solubilizing fungus.

contains Azotobacter,

Azospirillum

andResults:
Table 1. Number of tillers under Conventional rice cultivation and SRI with different inorganic and bio-organic
fertilizer (BIOST) combination (Muchlis, 2010)
Plant age (days after transplanting)Treatments

14

28

42
Plants per hill

56

70

-1

Rice Cultivation
Conventional

7,7a

18,8a

19,1b

16,5b

15,7b

SRI

2,2b

6,3b

24,9a

28,1a

25,8a

Without Fertilizer

4,5a

10,5a

19,6b

20,9b

16,9b

100% Inorganic NPK

5,1a

13,2a

22,7ab23,7ab22,8a

75% Inorg +200 kg Bio-organic Fert.

5,0a

13,3a

22,4ab23,6ab21,0ab

50% Inorg +200 kg Bio-organic Fert.

5,7a

13,9a

24,5a

25,3a

23,9a

50% Inorg

4,5a

11,9a

19,8b

20,4b

19,4ab

Fertilization

6

Table 2. Effect of rice cultivation system and combination of in-organic and bio-organic fertilizer on yield of rice
Yield at HarvestingFertilization

Conv

Dry Yield

SRI
ton ha

Conv

SRI

-1

Without

4,50f

5,69d

3,42f

4,44d

100% Inorg

6,13c

7,75a

4,90bc

6,72a

75% Inorg +200kg Bio-organic Fert.

5,55d

6,49b

4,40cd5,16b

50% Inorg +200kg Bio-organic Fert.

6,01c

7,94a

4,62cd6,77a

50% Inorg

4,98e

6,09c

3,83e

4,99b

Average

5,43b

6,79a

4,23b

5,61a

Table 3. Effect of rice cultivation system and combination of in-organic and bio-organic fertilizer on N, P and K
up-takeUp-takeTreatments

N

P

K
-1

g hillRice Cultivation Method
Conventional

0,182b

0,024b0,219aSRI

0,308a0,037a0,243aWithout

0,093c0,011c0,102c100% Inor

0,274b

0,031b0,258b

75% Inorg +200 kg Bio-organic Fert.

0,298b

0,033b0,242b

50% Inorg +200 kg Bio-organic Fert.

0,381a0,049a0,374a

50% Inorg

0,164c0,019c0,161bc

Fertilization

Figure 1.
The performance of subtitution of NPK inorganic
fertilizer with biofertilizer at conventional ricecultivation (left) and SRI rice cultivation methods

Conclusion:
1.

Bio-organic fertilizer application can replaced 50% inorganic NPK fertilizer with the same yiled as 100%
NPK Inorganic Fertilizer.

2.

The effect of bioorganic fertilizer is more in SRI cultivation method compared to conventional ricecultivation method.

7

Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer Project

Biofertilizer: showing its presence in the Malaysian
agricultural scene
Khairuddin Abdul Rahim, Malaysian Nuclear Agency (Nuclear Malaysia)

The year 2011 has seen significant inroads byorganism. MYAGRI is also one of Malaysia’s

biofertilizers in the Malaysian agroindustry, throughbiofertilizer companies actively utilising Nuclear

determined efforts by researchers in the field over

Malaysia’s

the years, and the increasing acceptance by the

MINTec-SINAGAMA, to irradiate substrates in its

plantation industries involving the country’s mostbioproducts formulations. In the Bioinnovation

important commodities, oil palm and rubber.

Award at BioMalaysia 2011, MYAGRI bagged ‘The

Through the platforms of Sustainable Development

Most Promising Innovation Award’ for one of its

and Green Technology, biofertilizer has positioned

bioproducts used in rice and other crops.

itself well, and with proper support throughR&D involving biofertilizer microorganisms has

continuous R&D and innovation in the public andalso flourished in the past year. At the home front,

private sectors, it will provide an impact inNuclear Malaysia received a ScienceFund grant for

Malaysian agriculture. It is indeed common

the project “Elucidating interactions of phosphate

nowadays to see biofertilizer products displayed inbiofertilizer on rhizobacteria and their effect on

expositions or exhibitions and being entered in

uptake of phosphorus and nitrogen using isotopic

competitions on innovation and new products

tracer technology”. This two-year project willawards. That this undertaking is also supported byproduce at least one multifunctional biofertilizer

traditional users of chemical fertilizers and otherformulation, and one of its target crops is rice

agrochemicals, is indeed refreshing, and gives agrown under minimal water condition. Most of theglimmer of hope for further successes.

rice in Malaysia is cultivated under the flooded

Malaysian Agri Hi-Tech Sdn. Bhd. (MYAGRI), thesystem, where much water is being utilised underbioproducts company with BioNexus status, which

regimented government-aided irrigation schemes.

utilisesa

The mutation breeding of rice to suit cultivation

biofertilizer inoculum generated through R&D ofunder minimal mater regime or aerobic condition

Malaysian Nuclear Agency in one of its products,

provides a new potential area where biofertilizer

agriCare® ORGANIC-N, produced about 40 t of thecanproduct in 2011, with a reportedly increasing

complementing the agrochemical inputs. Under

demand until the present. B. megaterium is well

aerobic

known of its phosphate solubilising properties.

microorganisms could be tested, including the

However, in our earlier study it has been shown toarbuscular mycorrhiza. This opens a new phase for

exhibit biological nitrogen fixing capabilities, thus

biofertilizers in the coming years. The universities

making it a potentially multifunctional biofertilizer

arecultureofBacillus

megaterium,

8

play

also

gamma

an

irradiation

importantcondition,

actively

role,

many

conducting

facility,

especiallyinbiofertilizer

researchonbiofertilizer organisms, through the Fundamental

At national level biofertilizer has also being

Research Grant Scheme and others, targeting on

positioned in new projects on the scopes of Green

multifunctionality, namely, plant growth promoting,Technology, Food Production Technology anddinitrogen

phosphate solubilising andInnovation, Crops Adopting to Climate Changes

potassium solubilising, for crops such as oil palm,

and the current development project under the

pepper, vegetables and rice. This bodes well for theTenth Malaysia Plan (2011-2015). The future looks

agriculture industry as there are still manybrightindigenous microorganisms with potential to be

agriculture sector.

fixing,forbiofertilizer

and

the

Malaysian

explored and developed.

(source: MYAGRI)

Product from MYAGRI utilizing Bacillus megaterium cultures
produced by Malaysian Nuclear Agency(photograph, source: MYAGRI)

9Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer Project

Bio NTM: Biofertilizer Technology as an Alternative Source toNitrogen Requirement of Tomato and Eggplant
Julieta A. Anarna, University of the Philippines Los Baños (UPLB)Block design (RCBD) with 4 replications using theIntroduction
andFPA protocol. The plot size was 10 square meters andeggplant (Solanum melongena) are the two mostthe seedlings were distanced at 50 cm. between hills.

important crops produced by the Filipino farmers.

Each furrow had 10 plant population.

These solanaceous crops require large amount of

The following treatments were used in the experiment:

Tomato(Lycopersicum

esculentum

Miller)nitrogen. Most of the farmers cannot afford to apply

T1 = Control (unfertilized, uninoculated)

the right amount of nitrogen fertilizer due to its highT2 = Recommended Rate of Chemical Fertilizer(RRC)

cost, hence they cannot get better yield.
The application of Bio N a microbial inoculant in

T3= ½ RRC

powder form that contains 2 species of nitrogen-fixing

T4 = ½ RRC + Bio N

bacteria have been recognized by Filipino farmer. Bio

T5 = Bio N

N, the nitrogen fixing inoculants that were initially

T6 = Full RRC + Bio N

developed for rice and corn have now been of interest

alone

Significant Findings

for use in other test crops to improve the yield and at

Tomato: The results on

the same time reduce the chemical nitrogen input.

tomatoThe need for generating more data on the response of

conducted

at

BIOTECH-UPLB statistics

Bio N to new crops are very essential to convince

showed that all the treatedmore farmers on the Bio N usage.

plantsMaterials and Methods

demonstrated

anincrease in yield over theSeeds of tomato c.v Atlas and eggplant c.v Casino

control or untreated plants. The particular variety

were sown in trays containing sterile coconut coir dust

under the Biotech demo site can only produce 20 tons

and garden soils as the germination medium. Twoof fruits if unfertilized. Inoculation with Bio N canweeks after, both tomato and eggplant seedling was

yield 27.83 tons/hectare, which is 35.77% more thanpricked/ transplanted to plastic cups and hardened forthe unfertilized plants. The highest was observed in

5 days before these were transplanted to the field. The

plants that was fully fertilized which is 38.6 tons per

experimental field area was at BIOTECH demo farm.

hectare, fallowed by ½ recommended rate of nitrogen

The amount of fertilizer that was used in this field test

fertilizers rate in the presence of Bio N. This field

was based on the general fertilization recommendation

data once more confirmed the results from theused by the Institute of Plant Breeding in the absence

previous field trial using c.v Diamante as the test plantof soil analysis. This consisted of a basal applicationthat inoculation of tomato with Bio N in theof 10 grams/hill of complete fertilizer (14-14-14). Tenpresence of modest amount of inorganic fertilizer candays later and 2 weeks thereafter, 1 tbsp of a

produce a yield comparable of higher than the fully

combination of 2 parts Urea (46-0-0) and 1 part

fertilized counterpart which means a saving of half ofmuriate of potash was applied as side dress to eachthe required fertilizer input. The yield responses toplant.

treatments were within the range of the national

The trial was conducted at the BIOTECH Demo farmpotential average of 20-30 tons per hectare.

and the set up was laid in a Randomized Complete

10Table1. Effect of chemical fertilizer and Bio NTN inoculation on yield of tomato c.v Atlas under the BIOTECH
demo farm, DS 20011.
Treatment

Yield (ton/ha)% Inc. Over Control

T1 – Control

20.50 B-T2 – Full Recommended Rate

36.29 A

77.03

T3 – ½ Recommended Rate

24.91 B

21.54

T4 – Bio N

27.83 B

35.77

T5 – Full Recommended Rate + Bio N

38.6 A

88.13

T6 – ½ RR + Bio N

37.8 A

84.47

C.V – 16.32%
Eggplant: The results (Table 2) show that the yield of eggplant increased in alltreatments over control. However, the increased in yield varied in all treatments.
The highest yield 7.2 tons per hectare was recorded in fully fertilized treatmentswith the presence of Bio N with 91.82 % over the control and has a 38%
increase in yield over full recommended rate of fertilizer. The next higher yield
was obtained from Treatment 6 with 84.72 % increase over the control and
31.74 % increase over Treatment 3. Although the yield response of each treatment
were not within the national potential average of 30 - 40 tons per hectare, theresults of the study revealed that Bio N contributed to the yield of the test plantin this experiment.
Table 2. Effect of chemical fertilizer and Bio N inoculation on yield of eggplant c.v Casino under the
BIOTECH demo farm, DS 20011.
Treatment

Yield (ton/ha)% Inc. Over Control

T1 – Control

3.75 C-T 2 – Full Recommended Rate

5.22 BC

39.07

T3 – ½ Recommended Rate

5.26 BC

40.31

T4 – Bio N

5.95 AB

58.61

7.2 A (38%)

91.82

6.93 AB (31.74%)

84.72

T5 – Full Recommended Rate + Bio N
T6 – ½ RR + Bio N
c.v – 16.97

Summary and Conclusion
The two field experimental set ups for tomato and eggplant were conducted at BIOTECH-UPLB demo farm basedon soil analysis. The treatments that were used for field trial at BIOTECH demo farm were control (no fertilizer,
no inoculation), full recommended rate, ½ recommended rate, ½ recommended rate plus Bio N, Bio N alone
and full recommended rate plus Bio N. The experiments were laid out in Randomized Complete Block Design(RCBD) with three replications. The data were subjected to statistical analysis (ANOVA) and Duncans Multiple
Ranger (DMRT) in case of treatment differences at 5% level of significance.
Based on the results from the experiments it is concluded that Bio N inoculation complement the amount of
nitrogen fertilizer requirement of the test plant was comparable to the yield of fully fertilized plots. It also showedthat Bio N inoculation response to modest amount of N fertilizer.
References
Biotech - UPLB Core Project Terminal Report, 2010-2011
Institute of Plant Breeding fliers, UPLB, 200811

Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer Project

Biofertilizer Status in Thailand
Sompong Meunchang, Department of Agriculture (DOA)
Biofertilizer is the material consisted of the

In 2007, irradiation technology was agreed to beliving microorganism which is able to supply theuse for development of biofertilizer innovation in

nutrients to the plant. In Thailand, the rhizobium

Department of Agriculture (DOA). DOA uses

biofertilizer is the first biofertilizer product and

gamma ray for carrier sterilization of rhizobium

the output rose to 200 tons/year in 1995.

biofertilizer, which expands production potential

At present, we developed 4 types of biofertilizer

by increasing the survival number for one log

product; 1) rhizobium 30 tons, 2) PGPR 5 tons 3)

and maintaining period to 24 months. In 2011,

mycorrhizal 5 tons and 4) phosphate solubilizer 2

we use 50 kGy of electron beam for carrier

tons. These biofertilizers are used for different

sterilization and 30-150 Gy for developed mutant

variety of crops. Rhizobium recommended

strains of Azotobacter and Beijerinckia. As aapplying with 44 genera of leguminous plant.

result, we got 5 strains of Azotobacter beijerickia

PGPR biofertilizer recommended applying withand 5 strains of Beijerinckia mobilis whichmaize,

Mycorrhizal

dinitrogen fixation ability measure by ARA wasbiofertilizer recommended applying with 22

higher than parent strain 50%. For meet the highgenera of plants, Now; it was said that the very

efficiency biofertilizer application in the field.

popular mycorrhizal biofertilizer is for rubber

We would focus on the specific plant andtree. The phosphate solubilizing biofertilizer

location of biofertilizer application research andrecommended applying with maize, vegetabledevelopment.

and fruits.

biofertilizer with each variety of plant and basic

rice

and

sugar

cane.

Becauseofthespecificoffertility of soil are main factors for responsibilityof all biofertilizer groups.

Fig 1 Seed coated of maize with irradiation carrier of PGPR biofertilizer (a)and the early growth respond of maize seedling at 10 days. (b)12

Forum for Nuclear Cooperation in Asia (FNCA) Biofertilizer Project

Multi-functional Biofertilizer
Research and Development in Vietnam
Pham Van Toan, Ministry of Agriculture and Rural Development (MARD)
Multi-functional biofertilizer play an important role

In 2011 some main activities are concentrated

for sustainable agricultural production in Vietnam.

on:

Research and development of multi-functional

a. Biofertilizer production: Establishing thebiofertilizer

carried

out

in

many

multi-function biofertilizer production units

research

in provinces.

organizations and universities. From 2008 to 2011
more than 35 new N-fixing, P-solubilizing, plantb. Field experiments and demonstrations:

growth promoting microbial strains are selected andcarrying out 4 experiments to evaluate theusedTechnologies

forefficacy of multi-functional biofertilizer on

production

areproduction of groundnut, vegetable, coffee,

developed and transfer to 6 production companies in

pepper and 5 demonstrations to show todifferent locations. Multi-functional biofertilizer

farmers the effects of multi-functional

incorporated with organic fertilizer are applied forbiofertilizer on growth, yield and root

rice, groundnut, soybean, vegetable, cotton, coffee,

disease control of groundnut, vegetable,

pepper and forestry trees in different locations andcoffee and pepper. Size of experiments andable to increase crop yield from 10 to 35%, reduce

demonstrations are 200 - 300m2 and 1 - 3

plant root disease and save 20 - 30% of required

ha.

for

production.

multi-functional

biofertilizer

chemical N and P fertilizers. In frame work of
FNCA

biofertilizer

multi-functional

project,

plan

for

Results

researchandresearch and development in Vietnam in 2011

thebiofertilizerofmulti-functional

biofertilizer

development in Vietnam during 2008 - 2011 iscan be summarized as follows:

follows:

a. Biofertilizer production: The results ofa.

Selectionofthecombinationofstudy on the capability of biofertilizer

microorganism having the activity related

production in different locations showed,

to plant nutrition, plant growth promotingthat it is possibble to establish thesubstrate, plant and soil health;multifunction biofertilizer production unit

b. Studying the carriers from irradiatedin NgheAn, QuangNam Quangtri, Daclak,material;

Daknong and Binhphuoc provinces. Withc. Evaluation of the benefit of microorganism

the fund from Vietnam Ministry of Scienceto plant and soil health;d.

Production

andand Technology 6 biofertilizer production
applicationofunits are established for 6 provinces in

multifunctional biofertilizer;

2010-2011. Multi-function biofertilizer are

e. Promoting the expand of biofertilizer use.

applied for groundnut, vegetable, coffee andpepper.

13

b. Effect of Multifunction biofertilizer

pepper, coffee, increase the yield and bring- Multifunction biofertilizer can improve thehigher benefit for the farmers in Quangtri,

growth

and

yield

of

groundnut

andDaclak, Daknong and Binhphuoc provinces.

vegetable which can replace the N mineralc. Promotion on biofertilizer production andfertilizer without the significant change ofapplication: 20 technical training courses are

groundnut and vegetable yield in Ngheanorganized in NgheAn, QuangNam Quangtri,province.

Daclak, Daknong and Binhphuoc provinces. 60- Biofertilizer incorporated with organic

technicker and 600 farmers are qualified in

fertilizer can reduce the the root disease of

biofertilizer production and application.

Effect of multifunctional biofertilizer on groundnut yield in Nghean province in 2011
Yield (tons/ha)
Place

Biofertilizer

Farmer practis (FM)% Increase to FM

Nghi Khanh – Nghi Loc

4.01

3.31

21.14

Dien Phong - Dien Chau

4.53

3.82

18.59

An Hoa - Quynh Luu

4.42

3.64

21.43

Averadge

4.33

3.59

20.61

Production of multi-functional biofertilizer

Multi-functional biofertilizer application to the crops

14