YMC USER'S MANUAL

Column Care and Use Instructions

J’sphere ODS-H80
J’sphere ODS-M80
J’sphere ODS-L80

1. Introduction
Thank you for purchasing a YMC high-performance liquid chromatography (HPLC) column. There are three ODS types in J’sphereseries and each has different surface coverage. All use the silica-gel with lower metallic impurity and are end-capped with our own
original technology. As these three types have different surface condition, they differ in separation property.
ODS-H80: Remarkably high hydrophobicity and surface coverage with excellent chemical resistance to acid and alkali.
ODS-M80: Moderate hydrophobicity and versatile type.
ODS-L80: Extreme low hydrophobicity, which is similar to YMC-Pack C8.
YMC HPLC columns, which are manufactured under highly controlled conditions, must pass a series of stringent tests before being
accepted for shipment. (Please refer to the column inspection report). To ensure optimal performance and durability of the column,
please read these instructions carefully before using this column.

2. Column connections
The “WT” or “PTH” at the end of the product code indicates the style of column endfittings.
WT = Waters style / PTH = Parker style

3. Shipping solvent
100% Acetonitrile. Replace with this solvent for storage. When replacing a mobile phase containing buffer salts/additives, extra caremust be taken to prevent salt precipitation.

4. Mobile phase
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ Aqueous or non-aqueous solvent can be used as a mobile phase. Repetitive replacement among solvents with large difference inpolarities might degrade the column performance. In general, acetonitrile, methanol and tetrahydrofuran (THF) are recommended forregular use. When using THF as a mobile phase, be mindful of the solvent resistance of your system or tubing (PEEK parts areespecially unsuitable for use with THF).
・ Recommended pH ranges of the column are between 2 – 7.5. For ODS-H80, pH ranges of 1 – 9 are available.
・ When using the column at pH near the upper or lower limit, a mobile phase containing 10% concentration of organic solvent should
be used. The column lifetime will shorten under certain conditions by temperature and mobile phase composition.
・ When replacing mobile phases, make sure of the miscibility among the organic solvents and take care to prevent the precipitation ofbuffer salts/additives to avoid overpressuring the column.

5. Column cleaning (general method)・ Flush the column with solution containing a higher ratio of organic solvent for washing out the compounds that have a great capacity
for retention in the column after using mobile phases not containing buffer salts/additives. Usable concentration of organic solvent isup to 100%. A cleaning solution containing THF might be effective when removing highly hydrophobic (lipid-soluble) substances thatare adsorbed onto the gel.
・ When using mobile phase containing buffer salts/additives, first replace with a water/organic solution containing no buffer
salts/additives (A ratio of water to organic solvent should be set at the same proportions as a mobile phase). Then flush the column inaccordance with the method described above. Mobile phase containing about 50 mM or less in buffer salts/additives can bereplaced directly with 60% acetonitrile aqueous solution.
・ Flushing with 100% water after using the column around the pH limit might shorten the column lifetime. Flush the column withwater/organic solution as described above, such as 60% acetonitrile aqueous solution.

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YMC USER'S MANUAL

6. Other environments
・ The operating pressure should be kept under 20 MPa (2900 psi) for 150 mm length column or less, under 25 MPa (3625 psi) for 250
mm length column, under 10 MPa (1450 psi) for 75 mm length column, under 10 MPa (1450 psi) for 10 mm I.D. column or more.
・ To prevent exposure of the column to excessive pressure, the sample solution should be filtered through a 0.2 μm membrane orsmaller to remove particulates. We recommend using a pre-column filter to prevent the column frit from being clogged with samples.
・ Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
・ Adjust the flow rate appropriately because the pressure changes depending on the column length, temperature, types of organic
solvent etc.
・ When using a small inner diameter column for study of preparative separation conditions, we recommend low flow rate tosimplify the transfer to a semi-preparative column.
・ The upper limit of column temperature is 50 °C. However, we recommend using the column at 20 – 40 °C, because column lifetime
varies depending on conditions such as pH.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM230123CE) 2/2