FNCA Mutation Breeding Project
Five Years Plan of Sub-Project on improvement of Composition or Quality in rice (2008ver.)CountryBangladesh

FY2007

FY2008FY20091) Collection of salt tolerant rice1) Collection of seeds from M2 plants

1)

landraces from coastal regions.

and raising of M3 population in theputativeexperi- mental field for screening

generation in the saline soil oftheand selection.

coastal region for high protein,region.

landraces.

2) Irradiation of embryogenic calli

high amylose and high yield.

2) Raising of M3V3 in the fields

3) Irradiation of seeds by different

with different doses (20-60 Gy) of2) Raising and selection of M1V1of saline affected coastal areas

doses (250-400 Gy) of gamma ray.

gamma rays.

plants in NaCl-free soil to getfor selection.

4) Raising of M1 generation in the3) Maintenance and scree- ning of

M2V2 seeds.

3)experimental field. M2 generations are

calli onto MS/N6 medium added

3) Screening and selection of M2V2

plantsbeing raised in the field as well aswith 0.5-1.5% NaCl for 120 days

plants for high protein, amylose

generations

fresh irradiations of Morichshail andwith periodical subculturing.

and yield in pot culture treated

content,

Ashfall were done and M1 are now4) Regeneration of M1V1 plants fromwithyield and salinity tolerance.

being grown in the field.

survivinggreenhouse condition.

5) Development of embryogenic calli

medium.

2)

Determination

amylosecontentofprotein

intheandcollectedfrom mature seeds.
6)

Establishmentofregeneration

system from embryogenic calli. In
addition, irradiated seeds of Takshoyl
were used to regenerate plants and 19
plants have been regenerated fromthose calli which are now growing in
the pot and are now at the booting
stage.

1

callion

FY2010

NaCl-freeRaising

FY2011and evaluation of1) Raising and evaluation ofmutants

mutants in M4 generation in

0.5-1.5%

NaClinM3

undersaline

soil

Evaluation
inofofthecoastalselectedsucceedingforproteinamylosecontent,

China1) Harvest M1 generation seed

1) Purification of breeding materials

1) Purification of M4 lines and make

1)

with

good

1) Possible combinations with

2) Selection of mutants with good(M3)cross with CMS lines and primary

restoringcrosseswithgoodplant character from M2 generation.

2) Analysis of amylose content fromevaluation of restoring ability.

more CMS lines for evaluation oftrails

selected M3 generation to screen M42) Growing F1 and F2 generation

heterosis.

2) Selection oflines with low amylose content

from cross of last yea and select2) Selection of

3) Select good M3 lines with low

good plant with desirable agronomic

with good plant character andcharacter.

amylose content to cross with thetraits.

primaryanalysis3) Amylose content analysis

content

inother varieties with good quality

MutantlinesabilityF4(F3、F4)

oflinesamylosegeneration

topreserve potential combinations

heterosis

lines

withenter(F5 、 F6)
goodplantfor selection of LAC
afterthen,fieldlines ,makecrossbetween selected LAC lines
andCMS lines in 2011 andevaluationofrestoring

ability and heterosis in 2012
and 2013.
Indonesia

1. Construction

1) Multiplication of seeds of pure1) Screening the lines for amylase

1) Breeding for high yielding(M3, M4).

lines

contents (Cont.)

and grain qualities of rice

indica varieties (IR36, IR64, Diah

Selection of early maturity and dwarf2) Screening the lines for amylose

2) Breeding for high yielding andvarieties using selected lines

Suci)stature.

contents

grain qualities of rice varieties(Cont.)

related to grain quality.

using selected lines

2) Writing final report

Selection of promising lines

Local adaptability trials andmaterials

ofcrossandbreeding

betweenuniqueelitejaponica

Purification

ofbreeding

materials

varieties (Pandan Wangi, Rojolele,(M5 lines)

andothercharacters

Koshihikari)
2. Development of M2 of KI 237 andKI 432.
Korea

Color rice1) Collection of breeding materials

1) Culturing M3 plants and selection

M4-5 generation field trial, analyses

fromofof functional compounds and related

foreignandnativerice

germplasm
2)

Irradiation

usefulmutants

2) Analysis of functional compounds:
ofradiation

molecular markers, and selection

Anthocyanin,(acute/chronic, gamma, ion beam)

Chrisanthemin(C3G;3) Harvesting M2 seeds

3-glucoside), tocoperol, etc.

cyanidin2

registration

Amylose library

JapanMalaysia

1) Ilpumbyeo: irradiation of gamma

Culturing M2 plants and selection of1) Culturing M3 mutant generation

Characterizationandline1)

Construction

ray with 250 dose

amylose mutants after analysis ofand reselection after analysis andselection

2) Harvested M2 seedamylose content and seed morphology

characterization

contents

2) Molecular analysis using amylose2)mutants

mutant lines for new variety

libraryofmutant

with various amylose

Selectionofpromising

1) Research method :1) Research method :1) Research method :1) Research method :Use existing amylose variants

Use mutagens such as gamma-rays

Use mutagens such as gamma-rays

Use2)Research Object :and ion beams

and ion beams

gamma-rays and ion beams

underRaise Koshihikari NILs relating to2) Research Object :2) Research Object :2) Research Object :conditions

amyloseScreen amylose variants from primary

ScreenScreen amylose variants from

2) Research Object :3)Expected achievements :varieties

primary varieties

primary varieties

Evaluate amylose library

Broadened amylose variants

3) Expected achievements :3) Expected achievements :3) Expected achievements :3) Expected achievements :Creation of amylose library

Creation of amylose library

Creation of amylose library

Completion of amylose library

Irradiation of seeds of advanced

Line screening of selected mutant

MutantYield trials of advanced mutant

Adaptability study of selectedlines of MR 211, MR219, and MRlines from M3-M4 Laboratory analysis(PCR-based) and field conditionlines Advance Yield Trial ofmutant line in several location

256,of amylose content andEvaluating the promising mutants

promising mutant line Quality

offor high yield and quality traits

evaluation of advanced mutant

varification(M5-lines for Milling quality,Test/Regional trial at Farmer

M6) To screen potential mutant

physical quality, chemical andplot Releaselines of MR 211, MR219, and MR

sensory

Q74withgamma

rays(re-irradiation) and ion beams Field

total

starchcontent.

Molecular

screening of M1-M2 populations

screeningwith

specific

At present 38 lines from M4 ofmicrosatelitemarker

amylosevariants

confirmation

frominlab

MR211 (300 and 400Gy) and MR219

Planting and 15,000 M1 seeds of(300 and 400Gy) were selected for

MR211 and MR219 (advanced lines)

256,

further testing.

using 300Gy and 400Gy and harvest of

Q74 with low amylase and total

M2 seeds

starchConducted preliminary analysis
for amylase content
Conducted molecular screening forspecific markers such as amylase

Preliminary
droughtlaboratory

resistancetest

usingforPEG

content.

Starchusing
molecular techniques.

(Polyethylene Glycol) at 0,12, 20, 30,
40 and 50% PEG

3profilingmutagens1) Research method :suchas

Evaluateamylosevariousgrainylibrary

cultivation

area

Local

Shoot elongation was inhibited at 40and 50% PEG
Greenhouse screening for minimalwaterrequirement

wasconductedusing MR211 (300 and 400Gy) andMR219 (300 and 400Gy)
From 500 lines tested, 55 lines
from M3 of MR211 (300 and 400Gy)
and MR219 (300 and 400Gy) wereselected based on average seed weightThe1) Irradiation of seeds

1) Identification of mutants in the M2

1)Selectionofmutantswith1)

Multiplication

Philippines

2) Determination of radiosensitivity

generation

improved grain quality in the M4 ndmutants

mutants with improved grain

to gamma radiation

2) Planting M3 generation

later generations

2) Determination of amylose andquality to the National Seed

3) Growing of M1 generation

3) Selection and Determination of2) Determination of amylose andprotein

Industry4) Planting of Httthe M2 generation

amylose and protein content and otherprotein content of selected mutants

mutants

registration

contentofdesirable

ofselected

Submissionofdesirable

Councilforgrain quality attributes.
ThailandLow Phytate in Rice
1) Genetics studies of Low phytic

1)other1) Continue yield, Fe content and1) Study on G x E and stability in1) LP mutant derived fromacid mutants derived from M6 ofagronomic traits evaluation of derived

other agronomic traits evaluation ofterm of Fe content in advance LP

SPR1 cultivar with high Fe

irradiated variety SPR1), RD23,LP mutant lines.

derived LP mutant lines.

mutantgood

bioavailability and acceptable2)2) LP molecular marker developing.

2)

and

cooking

population for Low Phytate (LP)3) Developing simplified protocol ofmutants combined with good grain

acceptable grain yield.

recommended.

traits.

breeding screening technique for highquality (eg. Low amylase, aroma)

2) Analysis of Fe content and2) LP mutants with good grain

3) Analysis of genes involved in

Fe content.

from newly generated population

cooking

quality to be continue selected

phytic4) Generate new mutant populations

using MAS.

mutants.

Generation

acidofbiosynthesis

MappingoftheYield,FecontentandScreening

forLow

Phytatelines

agronomic

performed
characters

qualityofeliteLP

generated population.

(using LP mutants as parents) for high3) LP mutants with good grain

Achievement: F2 progenies of aFe content.

quality to be selected for othercross between M6 mutant with Wild

agronomic traits by conventional

type were obtained to study genetic

pedigree selection.

4quality

willbein further breeding program.

mapping of lpa1
Low Amylose and Low Protein in Rice
●Low Amylose and Low Protein in

Additional work :1) Development of Glu A-I and GluM3 mutants with low glutelin and1)

Rice1) Developed a simplified method for

B-1 antibodies through recombinant

low amylose will be selected forglutelin and amylase will be1) Compositional analysis of Glu A

amylose content of the variants inproteindesireagronomic

traitsbyfurtherand Glu B in rice genetic stock (Thaiearly generation and contributed thedescribed by Tanaka (2004)

pedigreeselection

mean.

Low

local varieties) using conventional

other participated members.

2)

glutelin and low amylase still be2) M5 seeds will be derived

screening techniques (SDS-PAGE).

2) Seed multiplication of IR64 andtechnique using glutelin antibodies

maintainedand analysed for other desire

2) Chemical analysis for amylose

Koshihikari.

fortechnique previously developed.

content of the individuals with low3) Induce mutation in the varieties

breeding effort.

cooking quality

glutelin.

KDML105 (low amylose) , CNT1,3) Low glutelin and low amylose

tested for disease and insect

3) Data compilation for amylose

CNT80 and SPR1 (high amylose)mutants

resistance.

content and protein library.

using

selected.

Achievement: setting a protocol andAchievement: already done and M2

laboratory equipments.

progenies were obtained.
4)

gamma

ray

Mutants screening

irradiation.

forDevelop

large-scalestorageprotein low glutelin and low amylose
content using conventional technique.
5)

Identification

SDS-PAGE

withofglutelin

Coomassie

expression

byBluestaining and PCR in low glutelinmutants.
Achievement: waiting for low glutelinmutants

5(M2system

simplescreening

screeningplants)

as

will

and

be

using

screening

M4mutants

selectedwithfor

lowdesireagronomic traits.

grainqualitieseg.

aroma,and will beVietnam1) Field Trials for Red ST lines for1) Continue for pure line selections

1) Regional Acceptance of ST Red

1) Pure line selections for Ion

Yieldfor elite lines from ST16, ST19,lines

Beam Treated Rice cultivars- Quality: Amylose, Protein,… DirectST212) Regional Acceptance of ST

2) Pure line selections for LPA

determinations of aroma components

characters improved for regional

Combined lines and TDS 3.

(Dr. Long)-Cr,…

acceptance

M2 – M3 screening for Ion beam

3) Pure line selections for High(particularly high Fe content lines as

productions.

irradiatedricecultivars

forQuality (Dr. Do Khae Thinh)

new sources of germplasms)

2) Start with Ion Beam irradiation

non-photoperiodsensitiveand2) Field Trials for ST combined lines,

to traditional rice cultivars withsemi-warf plant type.

particularly following ST16, ST19,

special- Prof.Dr. Nguyen Thi Lang -ST21

conducted in Soctrang Province forMekong

And TDS 3 (an elite mutant line pure

further selections.

Institute, Can tho (Low Phytic

Minerals:

Fe,P,Zn,from Tamthom traditional cultivar)

populations

andquality:

forforM1

stablelarge

willscale

beDeltaRiceResearchAcid and High Quality Rice)- Dr. Do Khac Thinh – Institute ofAgricaluture

Sciences,

HoChiMinh City (High Yield andQuality Rice6