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18.Medicinal Benefits of Deep Sea Water on Metabolic Disease : Anti-diabetes
and Anti-obesity Effects
Byung Geun Ha, ○Yun Hee Shon
（Bio-Medical Research Institute、Kyungpook National University Hospital, Republic of Korea）

Metabolic syndrome is a clustering of at least three

lowered in HFD with BDSW fed group.

of five of the following medical conditions:

Furthermore, quantitative RT-PCR results revealed

abdominal obesity, elevated blood pressure,that the expressions of lipogenic genes and glucose

elevated fasting plasma glucose, high serum

oxidation-related genes are suppressed, while thetriglycerides, and low high-density lipoprotein

expressions of gene for glycogenolysis and for β-(HDL) levels. Recently, deep sea water (DSW) has

oxidation are increased in HFD with BDSW fed

been received much attention for its biological andgroup. In addition to, the expression of hepatic

clinical applications in the fields of health food,genes

cosmetics, and medicine. Several studies suggest

oxidation, and glycogenolysis was suppressed,that DSW may prevent metabolic disease,

while the expression of the genes involved inincluding obesity and diabetes. In present study,glucose uptake, β-oxidation, and glucose oxidation

we investigated effects of balanced deep-sea waterin muscle were increased in the STZ with BDSW(BDSW) on obesity and type 1, 2 diabetes in highgroup. In vitro, BDSW stimulated PI3-K, AMPK,

fat diet (HFD) induced obese, type 2 diabetes andand mTOR pathway-mediated glucose uptake instreptozotocin (STZ)-induced type 1 diabetes

C2C12 myotubes and 3T3-L1 adipocytes. BDSWmouse. BDSW was prepared by mixing DSW

increased AMPK phosphorylation in both cellmineral extracts and desalinated water to yield alines. In vivo, BDSW improved impaired AMPK

final hardness of 1000–4000 ppm. Consequently,phosphorylation in the muscles of HFD fed group

we found that HFD with BDSW fed group are

and STZ-induced diabetic group. Taken together,lowered body weight and plasma glucose level

these results suggest that BDSW has potentials ascompared to the HFD fed group and BDSW also

an anti-obesity and an anti-diabetic agent, owing

inhibited hyperglycemia in STZ-induced group.

to its ability to suppress increase of weight throughOral

anddecrease of adipose tissue weight, suppress

intraperitoneal glucose tolerance tests (IPGTT)

hyperglycemia, and improve glucose intolerance

showed that BDSW improves the impairedby modulating glucose metabolism, recovering

glucose tolerance in HFD fed group as well as

pancreatic islets of Langerhans and increasing

STZ-induced diabetic group. In histopathologicalglucose uptake. (This work was financiallyassay of liver, the HFD fed group showed severe

supported by the National R&D project ofsteatohepatitis, while HFD with BDSW fed group“Development of new application technology forwere similar with ND fed group. In the pancreas,

deep seawater industry" supported by the Ministry

BDSW restored the morphology of the pancreaticof Oceans and Fisheries of the Republic of Korea.)

glucosetolerance

tests(OGTT)

involvedin gluconeogenesis,

glucoseislets of Langerhans and increases the secretion ofinsulin both HFD fed group and STZ-induced

Keywords: Deep sea water, High fat diet, Obesity,

diabetic group. Moreover, triglyceride and total

Diabetes, Steatohepatitischolesterol levels in plasma and liver were(21)