Allergen managementwith bioavid lateral flow tests
Reliable allergen analysis and identification of also high positive samples

More information:

Perfect for cleaning controls

Direct analysis of rinse water samples (CIP water)

10/2022https://r-b.io/bioavid_EN

Versatile – also applicable for food testing

R-Biopharm • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: info@r-biopharm.de • food.r-biopharm.com

Allergen managementwith bioavid lateral flow tests

To prevent allergic reactions in sensitive persons, food production lines and the food itself have to be
free of residues. Lateral flow tests are a simple and fast technique to detect the presence or absenceof allergens.

Advantages

The hook effectHook line included to detect false
negative results

Everything included for hygiene
testing

Validated for selected foods

Fast and reliable results in 10 minutes

Suitable for everyone –even for non-laboratory staff

CH

The hook effect (or “overload” effect; Standard EN
15633-1. 2019) is observed when a very highamount of an analyte is present in the sample. In
this case, the analyte’s amount exceeds the amountof color-labelled antibodies and the ratio between
both needed for the formation of the test band
becomes imbalanced.
Although the analyte is present, only a faint or evenno test band is visible. Hence, there is risk for a
falsely low or negative interpretation of a test
result. This effect becomes visible by the hook line.
A missing hook line indicates high allergen content
in a sample. In this case, test users must notinterpret a missing test line as negative.

TC-line and H-line present, no T-line:
valid negative result
All 3 lines present: valid positive result

C-line and T-line present, H-line faint or absent:
valid high positive result
Only C-line present: suspected high positive,repeat test with higher sample dilution
No C-line: test is invalid

2

bioavid – test procedure BLH series

1 Sample preparationorSwab samples

CIP wateror

Food samplesoder

1 Homogenized

1samplePre-filled PBS vials(within the kit)

Subsample,water 1:10

CIPwaterCan dircetly be usedin the test procedure

22Centrifuge(2 min; 2,000 x g)
or filtrate

3 Intensively
flush out in thevial from 1

2 Test procedure
21
0.1 mL/3 drops
running buffer

0.1 mL sample

Mix (pipetting up and downor tapping the vial on the desk)

Reaction vial

3

4
Allergen test strip

Incubate 5 min
Incubate 5 min

5Read the result3bioavid – product overview

With hook line
ProductCoconut

Description

No. of tests/amountArt. No.

Lateral Flow Coconut incl. Hook Line

Qualitative detection of coconut residues

15 strips

BLH700-15

Qualitative detection of almond residues

15 strips

BLH701-15

Qualitative detection of brazil nut residues

15 strips

BLH702-15

Qualitative detection of mustard residues

15 strips

BLH703-15

Qualitative detection of hazelnut residues

15 strips

BLH704-15

Qualitative detection of macadamia residues

15 strips

BLH705-15

Qualitative detection of peanut residues

15 strips

BLH706-15

Qualitative detection of walnut residues

15 strips

BLH707-15

Qualitative detection of egg residues

15 strips

BLH708-15

Qualitative detection of sesame residues

15 strips

BLH709-15

Qualitative detection of cashew residues

15 strips

BLH710-15

Qualitative detection of pistachio residues

15 stripsBLH711-15

Qualitative detection of casein residues

15 strips

BLH714-15

Qualitative detection of crustacean residues

15 strips

BLH716-15

AlmondLateral Flow Almond incl. Hook Line
Brazil nut
Lateral Flow Brazil nut incl. Hook Line*
Mustard
Lateral Flow Mustard incl. Hook Line
Hazelnut
Lateral Flow Hazelnut incl. Hook Line
Macadamia
Lateral Flow Macadamia incl. Hook Line*
PeanutLateral Flow Peanut incl. Hook Line
WalnutLateral Flow Walnut incl. Hook Line*
Egg
Lateral Flow Egg incl. Hook Line
SesameLateral Flow Sesame incl. Hook Line
Cashew
Lateral Flow Cashew incl. Hook Line
Pistachio
Lateral Flow Pistachio incl. Hook Line*Casein
Lateral Flow Casein incl. Hook Line
Crustacean
Lateral Flow Crustacean incl. Hook Line

In addition to our tests with hook line, we also offer the following tests without hook line:

Without hook line
Soy
Lateral Flow Soy*

Qualitative detection of native soy residues

15 strips

BL612-15

Qualitative detection of milk residues

15 strips

BL623-15

Milk
Lateral Flow Milk
* soon available