O

P

E

R

A

T

EZ IQ

I

N

G

M

A

N

U

™

3000 Micro GC Chromatography Software
IPN 074-537-P1A

A

L

O

P

E

R

A

T

I

N

G

EZ IQ

M

A

N

U

3000 Micro GC Chromatography Software
IPN 074-537-P1A

www.inficon.com©2012 INFICON

re achus@inficon.com

A

LTrademarks
The trademarks of the products mentioned in this manual are held by the companies that
produce them.
Windows®, is a registered trademark of Microsoft Corporation.
All other brand and product names are trademarks or registered trademarks of their respective companies.

Technology Licenses
The hardware and/or software described in this document are furnished under a license and may be used or
copied only in accordance with such license.

Restricted Rights Legend
If software is for use in the performance of a U.S. Government prime contract or subcontract, Software isdelivered and licensed as "Commercial computer software" as defined in DFAR 252.227-7014 (June 1995), or
as a "commercial item" as defined in FAR 2.101(a) or as "Restricted computer software" as defined in FAR
52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or disclosureof Software is subject to INFICON standard commercial license terms, and non-DOD Departments andAgencies of the U.S. Government will receive no greater than Restricted Rights as defined in FAR 52.22719(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR
52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

Disclaimer
The information contained in this manual is believed to be accurate and reliable. However, INFICON assumes
no responsibility for its use and shall not be liable for any special, incidental, or consequential damages related
to the use of this product.
Due to our continuing program of product improvements, specifications are subject to change without notice.

Copyright©2012 All rights reserved.
Reproduction or adaptation of any part of this document without permission is unlawful.

EZ IQ Operating Manual

Table Of Contents
Trademarks
Technology Licenses
Restricted Rights Legend
Disclaimer
Copyright
Chapter 1
Using this Manual
1.1
1.2

Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
How to Contact INFICON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Chapter 2
Instrument Wizard
2.1

Offline Instrument Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

Chapter 3
Navigation Pane
Chapter 4

IPN 074-538-P1A

Program Architecture and Data Structure
4.1
4.2

About Method Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
About Data File Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Chapter 5
Opening and Saving Files
5.1
5.1.1
5.1.1.1

Open Data Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Open Data File Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

5.1.1.2

Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

5.1.1.3

Searching for Data Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

5.2
5.2.1
5.2.1.1

Open Method and Sequence Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Searching for Method and Sequence Files . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Save Data Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

TOC - 1

EZ IQ Operating Manual

Chapter 6
Reading CDF Files
Chapter 7
The Instrument Window
7.1
7.2
7.2.1
7.2.2

View the Instrument Activity Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
About the Instrument Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
Change View Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-3
Change File View Preferences. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-4

Chapter 8
Acquisition Parameter Setup
8.1
8.1.1
8.1.2
8.1.3
8.1.4
8.1.5
8.2
8.2.1
8.2.2
8.2.3

Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Configure the 3000 Micro GC Using the Classic Driver . . . . . . . . . . . . . . . . 8-1
Classic Driver Provision Flow Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-3
Classic Driver Method Control Parameters . . . . . . . . . . . . . . . . . . . . . . . . . 8-5
Classic Driver TCD Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-7
Classic Driver Trigger Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-10
Acquisition Parameter Setup with Enhanced Driver. . . . . . . . . . . . . . . . . . 8-11
Configure the 3000 Micro GC Using the Enhanced Driver. . . . . . . . . . . . . 8-11
Enhanced Driver Acquisition Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . 8-12
Enhance Driver Trigger Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-16

Chapter 9
9.1
9.2
9.3
9.4
9.5
9.6
9.7
9.8
9.9
9.10
9.11
9.12
9.13

TOC - 2

About the Chromatogram Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1
View Tiled or Overlay Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Zooming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-2
Scroll the Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-3
Add a Trace (Add a Single Chromatogram) . . . . . . . . . . . . . . . . . . . . . . . . 9-3
Add Multiple Traces to a Chromatogram Window . . . . . . . . . . . . . . . . . . . . 9-6
Annotate a Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-7
Change the Chromatogram Appearance . . . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Change the Axis Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-13
Change Data Graph Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-16
Set Up a Trace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-16
Remove a Trace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-19
Set Limits for X-Axis and Y-Axis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-19

IPN 074-538-P1A

The Chromatogram Window

EZ IQ Operating Manual

Chapter 10
Chromatogram Operations
10.1
10.1.110.1.210.1.2.1

About Chromatogram Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1
Move a Trace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-3
Stack Traces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-4
To Remove Offsets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-5

10.1.310.1.410.1.4.1

To Align Two Traces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-6
Stretch a Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-7
To Undo the Stretch, . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-7

10.1.510.2
10.2.110.2.210.2.310.2.410.2.510.3
10.3.1
10.3.210.3.3

Normalize Traces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-8
Perform Mathematical Operations on Traces . . . . . . . . . . . . . . . . . . . . . . 10-9
Smoothing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-10
Calculate Derivatives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-11
Add Two Traces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-12
Subtract Two Traces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-13
Multiply Two Chromatograms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-14
Utilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-15
Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-15
Copy to Clipboard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-15
Save Trace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-16

Chapter 11
Graphical Programming
11.1

About Graphical Method Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-1

Chapter 12
IPN 074-538-P1A

Data Acquisition and Control
12.1
12.2
12.3
12.4
12.5

Data Acquisition and Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-1
Single Run Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-1
Run a Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
Stop a Run in Progress . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-8
Extend Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-9

Chapter 13
Tutorial
13.1
13.2
13.3
13.3.113.3.213.3.3

Tutorial Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-1
Instrument and Method Wizards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-2
Create a Data Acquisition Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-3
Acquisition Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-3
Save the Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-5
Downloading a Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-6

TOC - 3

EZ IQ Operating Manual

Run a Preliminary Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-7
Set Integration Parameters Graphically . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-8
Create a Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-10
Calibrate Using a Stored Data File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-13
Create and Run a Sample Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-14
Run a Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-19
Review Multi-level Calibration Curves . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-20
Explore a Peak Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-21
Examine a Custom Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-22
Changing Integration Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-24

IPN 074-538-P1A

13.3.413.3.513.3.613.3.713.3.813.3.9
13.4
13.5
13.6
13.7

TOC - 4

EZ IQ Operating Manual

Chapter 1
Using this Manual
1.1 Introduction
This Operating Manual provides a basic operating overview and Tutorial for the
INFICON EZ IQ data system
This manual is designed to instruct new users on the acquisition of data andprocessing of results.

1.2 How to Contact INFICONWorldwide support information regarding:Technical Support, to contact an applications engineer with questions
regarding INFICON products and applications, orSales and Customer Service, to contact the INFICON Sales office, orRepair Service, to contact the INFICON Service Centeris available at www.inficon.com.

IPN 074-538-P1A

If you are experiencing a problem with your instrument or software, please havethe following information readily available before contacting INFICON:the serial number for the instrument,a description of the problem,an explanation of any corrective action that was already attempted,

and the exact wording of any error messages that may have been received.

1-1

EZ IQ Operating Manual

IPN 074-538-P1A

This page is intentionally blank.

1-2

EZ IQ Operating Manual

Chapter 2
Instrument Wizard
Each time an instrument application is initiated (by double-clicking the instrument
icon from the Main window), an Instrument Wizard will appear. This wizard isdesigned to direct the user through the basic functions of the instrument window.
See Figure 2-1.
Figure 2-1 Instrument Wizard

IPN 074-538-P1A

Table 2-1 Instrument Wizard Parameters

Create or modify a method

The Method Wizard steps the user throughcreating or modifying a method.

Create a sequence

The Sequence Wizard steps the user
through creation of an acquisition or
reprocessing sequence.

Run one sample

This button opens a window to use a stored
method to run a single sample.

Run sequence of samples

The Run Sequence window starts dataacquisition using a stored sequence.

Show at instrument startup

If this box is selected, the Instrument Wizard
will appear each time this instrument isstarted.

2-1

EZ IQ Operating Manual

2.1 Offline Instrument Wizard
To use an instrument offline, open the EZ IQ Offline program. The Instrument
Wizard will display the buttons shown in Figure 2-2 for creating methods,
sequences, or processing a stored sequence.

IPN 074-538-P1A

Figure 2-2 Instrument Wizard Offline

2-2

EZ IQ Operating Manual

Chapter 3
Navigation PaneA Navigation pane is displayed at the left side of the instrument window. See
Figure 3-1. The Navigation pane enables the user to quickly switch between the
major functions of the instrument window. A functional area can also be accessedby clicking on one of the function bars located at the bottom of the Navigation
pane.

IPN 074-538-P1A

Figure 3-1 Navigation Pane

3-1

EZ IQ Operating Manual

To turn the Navigation pane on
Click View >> Navigation pane.
To turn the Navigation pane off
Click the x button at the top-right of the Navigation pane.
To "park" the Navigation pane
The Navigation pane can be "parked" at the left of the Instrument window toprovide additional work space.
Once the Navigation pane is parked, it can be viewed again by moving thepointer over the Navigation tab. The Navigation pane will disappear when themouse is moved back into the work space.
To "park" the Navigation pane, click the push-pin button at the top of theNavigation pane.
The Navigation pane of the instrument window gives single-click access tomethod, sequence, report, control, and viewing options. The navigation function
bars at the bottom of the navigation pane open command trees that give access tocommands that are also available from the menu bar of the instrument window.
These commands are explained in Table 3-1.

3-2

Navigation Function Bar

Displays

MethodMethod commands, Data manual integration fixes, andTiled Display to display current data tiled with selected
Integration or Calibration (Peak Table) information.

Sequence

Edit.

Reports

Reports, Report template properties.

Control

Instrument Setup, Run Queue, Instrument Status.

Views

Data Display options. Manual Integration Fixes, Baseline
Check Status.

IPN 074-538-P1A

Table 3-1 Navigation Pane Parameters

EZ IQ Operating Manual

Chapter 4
Program Architecture and Data Structure
4.1 About Method Files
A method is used to acquire and/or reprocess a data file. A method contains
instructions for data acquisition (run time, sampling rate, etc.), integration,
calibration and peak information, reports, as well as optional functions such as data
export and user programs. Each method is capable of acquiring multiple
independent channels of data from a single chromatograph. Each channel canhave its own complete independent parameters, including sampling rate, run time,
integration events, external events, calibration, and reporting.
Although the method file is a separate file, the information contained in the method
is saved in the raw data file at time of acquisition. This way, the original method canbe reproduced, even if the method file was subsequently modified.

4.2 About Data File Structure
A data file is created whenever a sample is acquired, or when a data file is saved,
using Save As 32-bit…. The data file contains the following information:
File Information Header. Contains information such as the date and time ofacquisition.

IPN 074-538-P1A

Complete method parameters used to acquire and process the data (this isthe "original" method saved only when the data is acquired). Because multiple
channels of data can be simultaneously acquired on a given chromatogram, themethod section may contain complete parameters for more than one channel.
Raw data points for the run saved. Multiple chromatograms may be present in asingle data file, each of which represents a detector channel acquired for the run.
The raw data points are saved in binary format.
Results. The original integration results are saved in the file and can be recalled
later when the file is opened. In addition, the most recent analysis results andmethod are also saved in the data file and updated whenever analysis occurs. The
Sample ID for the results is also saved, as are manual integration fixes.
File Description. If a description was entered for the file, this text information isstored with the file, and can be viewed under the Data File Properties or from theOpen Data File window.
Instrument Configuration. The configuration of the instrument used to acquire
the data file is saved.

4-1

EZ IQ Operating Manual

Data File Audit Trail. An audit trail log is always saved in the data file that tracks
analysis of the data.

IPN 074-538-P1A

Data files are saved using the filename and extension specified when initiating thedata acquisition. The limit on filename length is 255 characters, including path.

4-2

EZ IQ Operating Manual

Chapter 5
Opening and Saving Files
5.1 Open Data FilesThe Open File iconon the command toolbar will open the Open File menu.

Whenever a file is opened using the data system, an Open data file window isdisplayed that allows opening the file and specifying parameters for searching, as
well as previewing, file contents. See Figure 5-1.
The Open data file window provides options for how to open and search for files.
The list of files of the type selected (Files of type) are shown. The files can beviewed as a list and their details can be shown by clicking the appropriate icon at
the top of the window. In addition, the chromatogram in a data file can be previewed
by selecting the Preview Chromatogram icon, or the file description can beviewed by clicking the Description icon. The * wildcard character can be used toview a list of certain file types. Files are stored in the current project folder. A folder
bar is included in the window to facilitate navigation.

IPN 074-538-P1A

Figure 5-1 Open Data File Window

5-1

EZ IQ Operating Manual

5.1.1 Open Data File Options
The Options region allows loading additional information at the time the data fileis opened.
5.1.1.1 Method
If Current is selected, the current method will not change when the data file isopened. When one of the other Method options is selected, the method selected
will be loaded at the time the data file is opened. From Results loads the method
used to create the selected results. Original/Acquisition loads the method used
for the original acquisition of the data file. This method will replace the current
active method.
5.1.1.2 Results
When one of the Results options is selected, the data file will be opened along withthe selected results. When a data file is opened with results, the integration andbaselines that generated those results will be displayed automatically when thechromatogram is drawn on the window. If Most Recent is selected, the data file will
be opened with the results from the last time the chromatogram was analyzed.
5.1.1.3 Searching for Data Files

Wildcards can be included as part of the file name searching. To perform a search,
fill in the field of interest for the Files of type to search, then click Find Now. For
example, by entering Test* in the Sample ID field and clicking Find Now, all thefiles where the Sample ID is Test followed by anything will be displayed. Click the
New Search button to clear the search settings and use new criteria for searching.
NOTE: When using the Search feature, make sure the Windows Hide File
Extensions for Known File Types option is turned OFF. To turn this off,click My Computer >> Tools >> Folder Options... and click the View tab.

5-2

IPN 074-538-P1A

There are options that allow display of only files of interest. Using Find files thatmatch these criteria, files containing certain information can be searched. All orpart of a Sample ID can be specified. Files may be searched by designatedAnalyst, by specific acquisition time frame such as Yesterday, Last 7 Days,
Today. Files may also be searched by specific modification time frame. These
criteria can be used one at a time or combined.

EZ IQ Operating Manual

5.2 Open Method and Sequence Files
To open a method or sequence file,

1 From the File menu, click Method or Sequence, followed by Open.
2 Open Method File and Open Sequence File windows are identical. Files are
stored in the current project folder. A drop-down menu is included in the window
to facilitate navigation. See Figure 5-2.
Figure 5-2 Open Method File Window

IPN 074-538-P1A

5.2.1 Searching for Method and Sequence Files
The criteria used to search for specific method and sequence files includes theselection of specific text found in the file description (Text in Desc.), Analyst
name, and date Created or last Modified.
NOTE: When using the Search feature, make sure the Windows Hide File
Extensions for Known File Types option is turned OFF. To turn this off,click My Computer >> Tools >> Folder Options... and click the View tab.

5-3

EZ IQ Operating Manual

5.2.1.1 Save Data Files
To save a data file,

1 Click File >> Data >> Save as 32-bit....
2 A Save data file as window opens to allow the user to browse to a location andthe the filename to be used to save the file. See Figure 5-3.
This command will save the current data file along with the current method in asingle file. This command is only enabled when the current data file is not in 32-bit
EZ IQ data format (such as 16-bit or converted files). In order to comply with goodlaboratory practices, the Save as 32-bit…command does not allow using same
name as an existing data file, unless the file is located in a public directory. A publicfolder is a folder where the path contains the term public. Data files in all other datasystem folders are protected from being overwritten and are stored within thecurrent project folder. A drop-down menu is included in the window to facilitate
navigation.

IPN 074-538-P1A

Figure 5-3 Save Data File As Window

To save current data in a new data file, enter the name of the new data file in theFile name field, then click Save. Use the icons at the upper right of the window toview details of a highlighted file, or to view the description of a highlighted file. An
entry Saved from <FILENAME> will be logged into the saved file as the first entry.

5-4

EZ IQ Operating Manual

IPN 074-538-P1A

If the Compress Data box is selected, the file will be saved in a compressed
format. Once saved in compressed format, it will automatically be decompressed
whenever the file is opened. However, once a file is saved in compressed format,
Save as must be used to save the compressed file back into its decompressed
format.

5-5

EZ IQ Operating Manual

IPN 074-538-P1A

This page is intentionally blank.

5-6

EZ IQ Operating Manual

Chapter 6
Reading CDF Files
When opening a CDF file, the software searches for one of the following Y-axislabels:

microvolts, uvolts, uv, uaumillivolts, mvolts, mv, mau

If one of these labels is not found, the software will try to read it from an AIA.ini
file, which is used to get multipliers for non-standard file types. If there is noAIA.ini file available, the software will try to make an estimate of the multipliers
based on the range of values. The data system determines the multiplier by picking
the largest number that will not overflow a 22-bit floating point number. It does thisby taking the largest value in the data stream and multiplying it by 1000000, 1000,
1 and picking the largest multiplier that does not overflow the 22-bit float.
If the CDF file being read is non-standard, the user will have to make an AIA.ini
file and put it in the data system program folder. The file should contain the Y-axislabel and multiplier and its format is as follows:[YLabelSection]
YLabelCount=<number of labels>
YLabelString1=<y axis label name 1>
YLabelValue1=<multiplier 1>
YLabelString2=<y axis label name 2>

IPN 074-538-P1A

YLabelValue2=<multiplier 2>

6-1

EZ IQ Operating Manual

IPN 074-538-P1A

This page is intentionally blank.

6-2

EZ IQ Operating Manual

Chapter 7
The Instrument Window
7.1 View the Instrument Activity Log
A log of activity from the current instrument is generated upon user operation. Toview this log, click File >> Instrument Activity Log >> Display Log. The
Instrument Activity Log window is displayed. See Figure 7-1. The Instrument
Activity Log window displays the instrument operator, the time the activity was
logged, and a description of the activity.
Figure 7-1 Instrument Activity Log

IPN 074-538-P1A

To view details of any line in the Instrument Activity Log, click on the line to highlightit, then right-click within the spreadsheet. From the drop-down menu, details of thehighlighted line can be viewed or printed. Select Print All to print the entire
Instrument Activity Log. In addition, the activity log can be exported, archived, or
purged. See Figure 7-2.
Figure 7-2 Instrument Activity Log Spreadsheet

NOTE: The Instrument Activity Log will record all activities. Over time, the size ofthe file containing the log will increase. To save space, the user may choseto select the Purge function to clear the log of all activities. The activity log
can be saved to another location by selecting the Archive function.

7-1

EZ IQ Operating Manual

7.2 About the Instrument Window
When an instrument is connected and EZ IQ is opened, the Instrument Window
will display. See Figure 7-3. From this window, all aspects of using the instrument
are accessible, including
Method development

Calibration

Sequence development



Instrument control and data acquisition

Analysis and review of data

Reporting

Data export

The appearance of the instrument window can be customized, adding or removing
Toolbars and Status information. These features are designed to make the systemeasier to use, and most users prefer to have them displayed.
Figure 7-3 Instrument Window
Instrument Menus

Toolbars

Chromatogram
Window

Navigation
Buttons7-2Graphical Events Buttons

IPN 074-538-P1A

Navigation
Pane

EZ IQ Operating Manual

7.2.1 Change View Preferences
To change the appearance of the instrument window,

1 Click View >> Preferences.
2 Select the General tab to change the general view preferences for the
instrument window. See Figure 7-4.
Figure 7-4 General Tab

IPN 074-538-P1A

Table 7-1 Preferences Parameters

General tab

Used to set up general preferences in the
instrument window.

Toolbar options

For each area of the window listed, Toolbar
and Tooltips (if available) can be turned off or
on. Click on the toolbar area, then select theShow toolbar and Tooltips boxes to enablethem.

Status bar options

Select the checkbox to turn on the statusbar. The status bar provides brief
information at the bottom of the instrument
window.

Time units

Select the time units for display ofchromatographic information.

7-3

EZ IQ Operating Manual

7.2.2 Change File View Preferences
To change the appearance of the instrument window,

1 Click View >> Preferences. The window shown in Figure 7-5 is displayed.
2 Select the Files tab to change the file view preferences for the instrument
window.

3 Select the file type, then enter the number of files to display in the Max Filesbox. This determines the number of recent files displayed in the File menu.

4 Click Clear Files to clear the current recent files list for the selected file type.
5 Click Clear All Files to clear the recent files for all the file types.

IPN 074-538-P1A

Figure 7-5 Preferences Window

7-4

EZ IQ Operating Manual

Chapter 8
Acquisition Parameter Setup
8.1 Introduction
EZ IQ is equipped with two 3000 Micro GC drivers. Both provide full control of the
instrument and differ mainly in the acquisition parameter setup.
3000 Micro GC Classic Driver
The Classic Driver was released when the 3000 Micro GC was introduced to the
instrument market. Support is provided for backward compatibility.
3000 Micro GC Enhanced Driver
The Enhanced Driver was developed later to provide significant improvement insoftware efficiency and graphical interface improvement. If a new 3000 Micro GC
is installed, it is highly recommended to adopt the Enhanced Driver.
Highlights on Enhanced Driver Update over Classic Driver

Significant reduction in prerun and postrun time required by the softwareIntroduced intuitive graphics for the "Instrument Status" window

The area count of the Classic driver is 100x times larger than Enhanced driver.
The Classic driver conducst a software multiplication on the area count while
the Enhanced driver does not implement this multiplier.

IPN 074-538-P1A

8.1.1 Configure the 3000 Micro GC Using the Classic Driver
Configuration of the 3000 Micro GC is required in order for EZ IQ to control the
instrument. Proceed as follows to configure a 3000 Micro GC.

1 Start the EZ IQ Config application (EZStartConfig.exe) from the Windows
Start menu

2 On the EZ IQ Configuration window, click Instrument Configuration …
3 On the Instrument Configuration window, enter the Instrument Name, click
Instrument Type, and then click Configure.

4 Click the INFICON 3000 Micro GC Classic icon to highlight it and then click
the green arrow to move the INFICON 3000 Micro GC Classic icon from
Available Modules to Configured Modules.

5 Double-click the INFICON 3000 Micro GC Classic icon in the Configured
Modules pane to open the configuration window.

6 Select the General tab.

8-1

EZ IQ Operating Manual

7 Enter information regarding the instrument configuration into the General tab,see Figure 8-1. For the system to automatically retrieve the configuration
settings, enter the Host Name or the IP Address and click Auto Configure.

Table 8-1 General Settings Parameters

8-2

Portable unit

Select when using a 3000 Micro GC portable
unit with a battery power source.

Remote Start

Select when an external start module has
been installed in the 3000 Micro GC.

Pressure units

Use the drop-down list to select the desired
units to be displayed for pressure.

Serial Number, Control module,
Channel A - B- C- D

These read-only fields display information
as obtained from auto-configuration.

IPN 074-538-P1A

Figure 8-1 General Settings Tab

EZ IQ Operating Manual
Table 8-1 General Settings Parameters (continued)

Auto Configure

Click to establish communications with the3000 Micro GC using the user specified
communications parameters. When
successful, the Serial number and Firmware
part numbers will be populated.

Communications

Specifies the addressing mode for TCP/IP
communications with the 3000 Micro GC.

8.1.2 Classic Driver Provision Flow Lines
Proceed as follows to configure information about the carrier gas flow lines for the3000 Micro GC.

1 Start the EZ IQ Config application (EZStartConfig.exe) from the Windows
Start menu.

2 From Instrument Configuration click Configure.
3 Click the INFICON 3000 Micro GC Classic icon to highlight it and then click
the green arrow to move the INFICON 3000 Micro GC icon from Available
Modules to Configured modules.

4 Double-click the INFICON 3000 Micro GC Classic icon in the Configured
Modules pane to open the Configuration window.

5 Select the Flow Lines tab and populate the fields. See Figure 8-2.

IPN 074-538-P1A

Figure 8-2 Flow Lines

8-3

EZ IQ Operating Manual

The following describes terms used in this window.
Table 8-2 Flow Lines Parameters

Second sample inlet

Specifies if a second sample inlet port isinstalled, which will allow a second
independent sample to be injected.

Carrier gas

Use to specify the type of carrier gas to be
used for analysis. This gas will be used for
all channels, unless the second carrier gas isenabled.

Second carrier gas

Used to specify if a second carrier gas line
has been installed.

8-4

Connected to

Use to specifty the channel to which thesecond carrier gas is connected. This field is
disabled if the second carrier gas control is
set to None.

Channel A/B/C/D

Use to specify the details of the input
streams for each 3000 Micro GC channel.
Depending on the Number of Channels
selection on the General Tab, some of thesegroups may be hidden.

Injector

Use to select the injector type—fixed orvariable.

ColumnUse to select the column installed for thechannel from the drop-down list. Available
column selections will depend on the type of
injector installed.

IPN 074-538-P1A

NOTE: This field is disabled if the numberof channels is less than 2.

EZ IQ Operating Manual

8.1.3 Classic Driver Method Control Parameters
Proceed as follows to set the method control parameters for the 3000 Micro GC.

1 Click Method >> Instrument Setup. See Figure 8-3.
2 Select the INFICON 3000 Micro GC tab.
3 Select the GC tab and populate the fields.

IPN 074-538-P1A

Figure 8-3 GC Tab

Table 8-3 GC Tab Parameters

Inject time

Used to specify the duration of the injection.

Post run time

Used to specify the duration of the post runpressure after analysis time.

Sample pump

Used to specify the manner in which thesample pump flushes the injection line.

Backflush

When applicable, used to specify time after
the injection that the injector should becleaned by backflushing.

8-5

EZ IQ Operating Manual
Table 8-3 GC Tab Parameters (continued)

Temperature control

Used to establish temperature set points forthe 3000 Micro GC heated zones. Select thecheckbox adjacent to the zone to enable thetemperature control and enter a value.

Pressure control

Used to set target pressures to be
maintained by the EPC (Electronic Pressure
Controller). Select the checkbox to enable
the pressure control and then enter thedesired values.
When the box is selected, the run will not
begin until the flow line has reached thetarget pressure specified by the Column
field and has maintained it for the time
specified by the Equilibration Time field.
After the run is complete, the flow linepressure will be set to the value specified by
the Post run field.

8-6

Equilibrium time

Specifies the time to wait to perform an
injection once the flow line has reached thetarget column pressure

ColumnUsed to specify the target pressure for theflow line.

Post runSpecifies the target pressure for the flow line
after the run has completed.

IPN 074-538-P1A

NOTE: The instrument will not run if thefilament is ON and the columnpressure is below 5 psi (34.5 kPa,
0.35 bar) in a method.

EZ IQ Operating Manual

8.1.4 Classic Driver TCD Parameters
To set the method control parameters for the 3000 Micro GC.

1 Click Method >> Instrument Setup.
2 Select the INFICON 3000 Micro GC tab.
3 Select the TCD tab for the desired channel.
4 Select Acquisition Channel On and populate the fields. See Figure 8-4 for an
example of a 2-channel 3000 Micro GC.

IPN 074-538-P1A

Figure 8-4 TCD - Channel A Tab

8-7

EZ IQ Operating Manual

Acquisition channel on

When selected, it will enable dataacquisition using the detector. When
cleared, fields except Filament on will bedisabled.

Filament on

When selected, the detector filament will beturned on prior to acquisition. When cleared,
it will be turned off.

Autozero

When selected, the detector output will be
adjusted to zero prior to data acquisition.

Baseline Offset

Specifies a fixed offset that will be applied tothe signal being returned by the detector.
This offset is applied prior to the start of therun. This field will be disabled if the
Autozero box is selected.

Sensitivity

Used to set the detector signal response
sensitivity.

Sampling

This is the rate at which data points arecollected and stored in the data file. When asampling rate is selected, text appears
below this field indicating the narrowest
peak width for which this sampling rate isadequate. The sampling rate may be
specified in terms of sampling frequency or
sampling period.

Sampling Frequency

Sampling Frequency is the rate at whichchromatographic data points are collectedfrom the Thermal Conductivity Detector(TCD) expressed in Hz. 20, 50, 100, 200 Hz
may be selected.
This field is enabled when the Sampling
Frequency option is selected. When
cleared, it is disabled.

8-8

IPN 074-538-P1A

Table 8-4 Channel A Tab Parameters

EZ IQ Operating Manual
Table 8-4 Channel A Tab Parameters (continued)

Sampling Period

Sampling Period is the rate at whichchromatographic data points are collectedfrom the Thermal Conductivity Detector(TCD) expressed in seconds. 0.005, 0.05,
0.02, 0.01 sec may be selected.
This field is enabled when the Sampling
Period option is selected. Otherwise, it isdisabled.
Determines the length of the analysis for thischannel.

Acquisition Delay

Use to enter the interval between the start of
the run (Trigger) and the time whensampling starts for this channel.

IPN 074-538-P1A

Run Time

8-9

EZ IQ Operating Manual

8.1.5 Classic Driver Trigger Parameters
The Instrument Setup window also provides a Trigger tab with a trigger Typeselection. See Figure 8-5.

Table 8-5 Trigger Tab Parameters

External

Data sampling starts when externallytriggered.

ManualUser inititated data sampling.

None

Sampling of data starts immediately whenStart is clicked.
Normally, select None for 3000 Micro GC.
When completed with the acquisition setup
information, click the X box in the upper righthand corner of the tab window to exit thewindow.

8 - 10

IPN 074-538-P1A

Figure 8-5 Trigger Tab

EZ IQ Operating Manual

8.2 Acquisition Parameter Setup with Enhanced Driver
8.2.1 Configure the 3000 Micro GC Using the Enhanced Driver
Configuration of the 3000 Micro GC is required in order for EZ IQ to control the
instrument. Proceed as follows to configure a 3000 Micro GC.

1 From the Windows Start menu, click the Chromatography folder. Open the
program EZ IQ Config (EZStartConfig.exe).

2 On the EZ IQ Configuration window, click Instrument Configuration …
3 On the Instrument Configuration window, enter the Instrument Name, click
Instrument Type, and then click Configure.

4 Click the INFICON 3000 Micro GC icon to highlight it and then click the Green
arrow to move the INFICON 3000 Micro GC icon from Available Modules to
Configured Modules.

5 Double-click the INFICON 3000 Micro GC icon in the Configured Modules
pane to open the configuration window.

6 In INFICON 3000 Micro GC Configuration window, see Figure 8-6, select theConnectivity tab, enter the IP Address of the instrument, and then click Load
Configuration from GC. The 3000 Micro GC configuration will load into EZ IQ.

IPN 074-538-P1A

Figure 8-6 Enhanced Driver - Load Configuration

7 Click OK to exit INFICON 3000 Micro GC Configuration window.
8 Click OK to exit INFICON 3000 Micro GC window.
9 Click OK to exit Instrument Configuration window.
10 Click Close to exit EZ IQ Configuration window.

8 - 11

EZ IQ Operating Manual

8.2.2 Enhanced Driver Acquisition Parameters
As part of a chromatographic method, acquisition parameters can be defined in theInstrument Setup window Navigation pane. See Figure 8-7.
Figure 8-7 Navigation Pane

Double-click Instrument Setup to obtain the Instrument Setup window. See
Figure 8-8 as an example for a 2-channel 3000 Micro GC.

IPN 074-538-P1A

Figure 8-8 Instrument Setup Screen

8 - 12

EZ IQ Operating Manual

The following are acquisition setpoints for the 3000 Micro GC (window tool tips will
provide valid data ranges).
Table 8-6 Acquisition Setpoints Parameters

IPN 074-538-P1A

NOTE: Regarding Sample Inlet, Injector and Column Temperature—Each heated zone
has an associated checkbox; if selected, the zone is enabled (heating), if cleared,
the zone is disabled (not heating). The setpoint value is preserved if the zone isdisabled. This capability is especially useful with a 3000 Micro GC Portable toreduce battery drain.
Sample Inlet Temperature

The temperature range of the sample gas as
it flows though the inlet. If two or more GC
modules share an inlet, only one
temperature field is displayed.

Injector Temperature

The temperature range of the sample gas as
it flows through the injector.

Column Temperature

The temperature range of the sample gas as
it flows through the column.

Sample Pump (On/Off)

Selecting this checkbox enables the sample
pump to run for a specified number of
seconds. Clearing Sample Pump disables
the pump, a capability which is especially
useful with a Portable 3000 Micro GC to
reduce battery drain.

Sampling TimeThe length of time (in seconds) allowed forthe sample gas to flow through the injector
before the start of the run. If two or more
3000 Micro GC modules share a sampling
pump, only one sampling time field will beavailable.

Continuous Sampling (On/Off)

Selecting this checkbox allows sample tocontinuously flow through the sample loop
before an injection is made. WhenContinuous Sampling is selected,
Sampling Pump will be off and Sampling
Time will not be available.

Inject TimeThe length of time (in milliseconds) allowed
for the sample gas to flow into the column
before the flow is stopped.

Run Time

The length of time (in seconds) allowed forthe detector to collect data and send it to thePC.

8 - 13

EZ IQ Operating Manual
Table 8-6 Acquisition Setpoints Parameters (continued)

Post Run Time and Post Run Pressure

A step-increased (not a ramp) pressure
push for a length of time (in seconds) toremove the remaining sample gas from thecolumn.

Pressure Equilibration Time

Length of time (in seconds) the channel willneed to stabilize at the pressure setpoint
before the system is ready for a run.
Pressure Equilibration Time begins when
the actual column pressure stays within 0.5
psi (3.4 kPa) of the column pressure setting.
Pressure Equilibration Time is not part of
the run time.

Column Pressure

The pressure of the gas flowing through thecolumn.

Detector Filament (On/Off)

Select the check box to turn the Detector
Filament On, or clear it to turn the Detector
Filament off. When the Filament is off, nodata is collected for the selected signal. Turnthe filament off before servicing the
instrument or changing carrier gas.

Detector Sensitivity

The sample concentration ranges areStandard and High.
Standard—Used to detect a wider range of
responses. This setting sets the dataacquisition to detect the widest range
possible without over-ranging.
High—Used to detect very small responses.
This setting sets the data acquisition to allow
detection of lower concentrations of sample
components.

Detector Data Rate

8 - 14

The number of data points the 3000 Micro
GC detector will collect every second. The
Default Data Rate setting is 50 Hz.

IPN 074-538-P1A

NOTE: Each column has an associatedcheckbox; if selected, the gas isenabled (flowing), and if cleared,
the gas is disabled (not flowing).
The setpoint value is preserved if
the gas flow is disabled. This
capability is useful for conserving
gas supplies when the column is
not in use.

EZ IQ Operating Manual
Table 8-6 Acquisition Setpoints Parameters (continued)

Baseline Offset

Sets the starting point for chromatographic
data within a range from -50 to 500 mV. The
default is zero.

Backflush TimeThe time (in seconds from the beginning of
the run), when the injector will backflush thesample gas in pre-column to vent.
NOTE: This setpoint is provided only for an
instrument channel with abackflush injector.

IPN 074-538-P1A

NOTE: The time entered may be equal toor less than the Run Time, up to
the maximum permitted run time forthe given instrument (2400
seconds for a 2-Channel
instrument or 1200 seconds for a
4-Channel instrument).

8 - 15

EZ IQ Operating Manual

8.2.3 Enhance Driver Trigger Parameters
The Instrument Setup window also provides Trigger Type selection. See Figure
8-9.
Figure 8-9 Trigger Type Selection

External

Data sampling starts when externallytriggered.

ManualUser must start the data sampling.

None

Sampling of data starts immediately whenStart is clicked in the Single Run window.
When the acquisition setup information iscompleted, click the X box in the upper righthand corner of the tab window to exit thewindow.

8 - 16

IPN 074-538-P1A

Table 8-7 Trigger Type Selection Parameters

EZ IQ Operating Manual

Chapter 9
The Chromatogram Window
9.1 About the Chromatogram Window
Data is displayed in the chromatogram window. Each channel will have its own
chromatogram window. It is possible to add multiple traces to a single
chromatogram window and perform comparison and mathematical operations onthem. To access specialized commands for the chromatogram window, right-click
anywhere within the chromatogram window area to display a list of commands.
These commands allow for a wide range of functions, including: adding multiple
chromatograms to the window, changing the appearance of the chromatogram,
adding annotations, adjusting X- and Y-axes, and performing mathematicaloperations. There is also an option to view or change the properties of thechromatogram. See Figure 9-1.

IPN 074-538-P1A

Figure 9-1 Chromatogram Window

The chromatogram window will display data in real time as a sample is running. At
the end of a run the data becomes the "current data". The appearance of thechromatogram can be changed by selecting annotations, fonts, and labeling.
These features can be accessed by right-clicking on the chromatogram andclicking Annotations. In the Utilities menu, the current window view can be
printed, copied to a clipboard, or saved in a file.

9-1

EZ IQ Operating Manual

9.2 View Tiled or Overlay Data
By default, each channel will have a chromatogram window. Chromatograms fromdifferent channels can be overlaid onto a single window by selecting View, thenOverlay Data. The chromatograms can be separated into multiple windows by
selecting View, then Tile Data. Individual channels may be zoomed in and theirappearances may be changed as described below.
In Tiled mode, window tiling preference can be set by clicking Window >>Cascade, Window >> Tile Horizontally, or Window >> Tile Vertically.

9.3 Zooming
It is possible to examine a chromatogram in more detail by zooming in on a portionof the chromatogram. Press and hold the left mouse button and drag open the box
so that it highlights the area of interest. Then release the mouse button. See Figure
9-2.

IPN 074-538-P1A

Figure 9-2 Zooming

To return to the previous level of zoom, double-click on the chromatogram. To zoom
to the full chromatogram again after multiple zooming operations, right-click
anywhere in the chromatogram window, then click Full Unzoom from the menu
displayed. Or Ctrl-Z or shift-double-click in the chromatogram window. Once thechromatogram is in a "zoomed" view, it can be scrolled. See section 9.4.
Time and Amplitude are displayed at the top of the chromatogram window. These
values change as the pointer is moved and reflect the time and amplitude of the
trace where the pointer is located. If there is more than one trace, the display canbe changed to another trace by clicking on the other trace. If the traces are
displayed in different colors, the color of the Time and Amplitude display willreflect the color of the trace displayed.

9-2

EZ IQ Operating Manual

9.4 Scroll the Chromatogram
Once zoomed in on a chromatogram, the chromatogram can be scrolled to the rightor left without losing the zoom. Press the CTRL+SHIFT keys and move the mouse
until the pointer changes to a hand. Press the left mouse button and drag themouse to the left or right.
The X- or Y- axis can be scrolled to view features which may be out of range. Press
CRTL+SHIFT while the pointer is outside the graph area, yet near the axis ofinterest. The pointer will change to an up/down arrow near the Y-axis, or a left/rightarrow near the X-axis. Moving the mouse in this mode will scroll the graph up/downor left/right on the axis.
To restore the original view in the chromatogram window, right-click then click Full
Unzoom.

9.5 Add a Trace (Add a Single Chromatogram)
The chromatogram window is used to view data, either current data (real-time) in
the instrument window, or saved data. Multiple chromatograms can be viewed in asingle chromatogram window. This is convenient, for example, to compare a past
run with current data or overlay an oven or pump profile.
To add a new trace to the chromatogram window,

1 Right-click anywhere in the chromatogram window. A menu will appear. See
Figure 9-3.

IPN 074-538-P1A

Figure 9-3 Chromatogram Window Options

9-3

EZ IQ Operating Manual

2 Click Add Trace…. The New Trace Properties window will display. See Figure
9-4.
Figure 9-4 New Trace Properties Window

3 Select the New Trace tab. Fill in the fields to add a trace to the chromatogram
window and set its properties. These properties apply only to the trace selected
and are not saved as part of the method. When a new trace is opened, theproperties will be set to default values. Added traces are normalized by default.

9-4

Data SourceEnter the name of the file from which to get
the trace. Or, click the File icon adjacent to
the field and select a data source.

Current Data

Selects a trace from the current
chromatography data.

Current Method

Selects a trace from the current method (ifavailable).

Open Data

Selects from stored data files in which a
trace can be selected for display.

Trace

Select the trace to be displayed. Click theicon to display available traces.

IPN 074-538-P1A

Table 9-1 New Trace Tab Parameters

EZ IQ Operating Manual
Table 9-1 New Trace Tab Parameters (continued)

Scale toSelect one of the scaling options:

Trace x

Scales to another trace in the window.

Autoscale to largest peak

Scales such that the largest peak is onscale.

Autoscale to 2nd largest peak

Scales such that the second largest peak is
on scale.

Autoscale to 3rd largest peak

Scales such that the third largest peak is onscale.

User Defined

Allows entering a value for Y max and Y min.

Normalized

Allows normalization of one trace to fit on thegraph.
If User Defined scale is selected, enter aminimum value for the Y-axis.

Y Max

If User Defined scale is selected, enter amaximum value for the Y-axis.

Units

Select the units for display.

X OffsetEnter a value in units for offset of the X-axis.

Y OffsetEnter a value in units for offset of the Y-axis.

Y ScaleEnter a multiplier that will be applied to theentire trace.

IPN 074-538-P1A

Y Min

9-5

EZ IQ Operating Manual

9.6 Add Multiple Traces to a Chromatogram Window
To add more than one chromatogram to the view,

1 In the chromatogram window right-click, then click Add Multiple Traces…. The
Open Data File window will display the traces that can be selected from the filelist. See Figure 9-5.

2 To add a file, either click on the filename, then click Add, or double-click on thefilename from the list.

3 Once a data file is added to the list, select the channel by clicking on the Trace
field, then click the down-arrow button. If multiple channels for that file areavailable, select the desired channel.

4 To delete a trace from the display list, click on its name or number, then click
Delete.

9-6

IPN 074-538-P1A

Figure 9-5 Open Data File Window

EZ IQ Operating Manual

5 To open multiple traces, click Open. The selected files/channels will appear in
the chromatogram window. See Figure 9-6.
Figure 9-6 Selected Files/Channels

9.7 Annotate a Chromatogram
To change the annotations on the chromatogram,

1 To view trace annotations, right-click in the chromatogram window and selectAnnotations to open the Trace Annotation Properties window. See Figure
9-7.

IPN 074-538-P1A

Figure 9-7 Trace Annotation Properties Window

9-7

EZ IQ Operating Manual

2 Select the trace from the drop-down list.
3 For the selected trace, click Peaks or Groups to select the type of annotation
to use.

4 Click on an Available Annotation. When an annotation is highlighted, add it to
the annotations to be displayed by clicking the green arrow key (pointing to theright). This can also be achieved by double-clicking the selection.

5 For certain annotations, the number of places to be displayed to the right of thedecimal point can be designated. Enter this value in the Decimals box for thehighlighted item.

6 Select the check box(s) to display Baseline, USP Width, or Retention Time
Windows, Show Undetected Named Peaks, and Group Ranges on the
trace.
NOTE: The Retention Time window annotation displays the window set in thePeak Table. This window is not adjusted for relative retention time.

7 Continue to select as many annotations as desired for this trace. When
finished, click OK.

8 Select or change annotation for an existing trace by a right-click in the

IPN 074-538-P1A

chromatogram window, then clicking the Annotation. The selections made will
apply to all traces open for this channel or until they are changed (via the OK
or Apply button). To apply the annotation changes to all open channels, click
Apply to All. Annotations are not saved as part of the method and areconsidered a function of the instrument application. If the method is closed thenopened, the current settings will apply.

9-8

EZ IQ Operating Manual

9.8 Change the Chromatogram Appearance
The appearance of the trace (line type, color, etc.) can be changed in the
Appearance tab in the Data Graph Properties window.
To change the appearance of a chromatogram or trace,

1 In the Chromatogram Window, right-click and select Properties. The Data
Graph Properties window is displayed. See Figure 9-8.

2 Select the Appearance tab.

IPN 074-538-P1A

Figure 9-8 Data Graph Properties - Appearance Tab

Table 9-2 Appearance Parameters

SchemeIf an appearance scheme has beenpreviously saved, select it from this box.
Save As… saves the existing appearance
scheme with a user defined name. Deletedeletes the scheme.

Item

This drop-down menu provides options ofwhich part of the chromatogram window tochange . The choices include the graph itself(including background and legends) and theavailable traces.

9-9

EZ IQ Operating Manual
Table 9-2 Appearance Parameters (continued)

Sub-item

Use the drop-down menu to select the
sub-item to modify. The choices will change
based on the item selected. For example, if
the item selected is the graph, setting up
appearances of sub-items including thebackground, axes and labels for the graph
are available. If the item selected is achromatogram data channel, access tosetting appearances of sub-items such asbaselines, start and stop tic marks, andannotation are available. If the item selected
is text, access to the font formattingcommands is available.
When a sub-item is selected, access tofields appropriate to that item are available.
For example, if Baseline is selected, thecolor and line type can be chosen. If
Annotation is selected, the font appearance
and color can be chosen.

IPN 074-538-P1A

Figure 9-9 Channel A Appearance

The appearance of any trace can be changed by right-clicking in the chromatogram
window, then clicking Appearance…. A window identical to the one shown above
for the Appearance tab will be displayed.
Sub-items available in the Appearance tab are shown in Table 9-3.

9 - 10EZ IQ Operating Manual

Table 9-3 Sub-items Available in the Appearance Tab

Item

Sub-Item

Description

Graph Background

Select the color of the graph background. Default isblack.

Graph Title

Select a color and/or font for the Title of the graph.
There must be a Graph Title defined in the Axis Setup
tab in order for it to appear in the window.

Graph Left Y-Axis

Select a color for the left Y-Axis.

Graph Left Y-Axis Major Ticks

Select a color for display of major unit marks on the left
Y-Axis.

Graph Left Y-Axis Minor Ticks

Select a color for display of minor unit marks on the left
Y-Axis.

Graph Left Y-Axis On/Off

Turns On or Off the left Y-Axis

Graph Right Y-Axis

Select a color for display of a right Y-Axis.

Graph Right Y-Axis Major Ticks Select a color for display of right Y-Axis major ticks.

IPN 074-538-P1A

Graph Right Y-Axis Minor Ticks Select a color for display of right Y-Axis minor ticks.
Graph Right Y-Axis On/Off

Turns On or Off the right Y-Axis.

Graph X-Axis

Select a color for the X-Axis.

Graph X-Axis Major Ticks

Select a color for display of major unit marks on the
X-Axis.

Graph X-Axis Minor Ticks

Select a color for display of minor unit marks on the
X-Axis.

Graph X-Axis On/Off

Turns On or Off the X-Axis.

Graph Legend

Select a color and/or font for display of the graphlegend. The legend indicates what traces are currently
displayed in the window. The Legend is turned On or
Off from the Axis Setup tab.

Graph Grid

Select a color for display of the grid lines. Grid lines areturned On and Off from the Axis Setup tab.

Data

Trace

Select a color and/or line type for display of the selectedtrace.

Data

Annotation

Select a color and/or font for display of the trace
Annotation(s). The items to be annotated for a trace are
selected in the Annotations tab.

Data

Baseline

Select a color and/or line type for display of thebaseline.

9 - 11

EZ IQ Operating Manual
Table 9-3 Sub-items Available in the Appearance Tab (continued)

Sub-Item

Description

Data

Baseline Start Tick

Select a color and/or line type for display of baseline
start ticks.

Data

Baseline Stop Tick

Select a color and/or line type for display of baseline
stop ticks.

Data

USP Width

Select a color and/or line type for display of the USP
Width, if calculated.

Data

RT Window

Select a color and/or line type for display of expected
retention time windows for named peaks.

Data

RT Window (undet.)

Select a color for display of RT Window for expectedpeaks that were not detected.

IPN 074-538-P1AItem

9 - 12

EZ IQ Operating Manual

9.9 Change the Axis Properties
The Axis Setup tab allows configuration of the appearance of the axis on thechromatogram. These settings apply to active traces. To change the Axis
properties,

1 In the Chromatogram window, right-click and select Axis Setup. The Axis
Properties window is displayed. See Figure 9-10.

IPN 074-538-P1A

Figure 9-10 Axis Properties Window

9 - 13

EZ IQ Operating Manual

Table 9-4 Axis Properties Parameters

Graph Title

Enter a title for the graph, if desired. Thisappears at the top of the graph.

Axis

From the drop-down list, select the axis ofinterest: Left Y-Axis, Right Y-Axis, or
X-Axis. Then choose the limits for the axis.
For Y-Axis selections, Use Limits of Trace
will get the limits from one of the traces in thewindow. Manually Set Trace's Limits will
box and set the Y-Axis limits to a user
defined range. If None, no Y-Axis values will
be displayed.
For the X-Axis, either choose to Autoscale,where the X-Axis is set to the longest trace.
Or, set an absolute range for the X-Axis byclicking Use This Range, then enter aminimum and maximum X-Axis value for the
trace. Click Get Limits to retrieve the X-Axis
range from the current trace.
Enter a value for the trace margins, inpercent, for top and bottom of the graph.

IPN 074-538-P1A

Margins

9 - 14

EZ IQ Operating Manual
Table 9-4 Axis Properties Parameters (continued)

General Options

Select the check boxes to turn graphannotations on and off. If the legend box isselected, the legend for a trace can beturned on or off from the Trace Properties
spreadsheet.

Figure 9-11 Chromatogram Window with Legend and Grid Displayed

IPN 074-538-P1A

Figure 9-12 Chromatogram Window with No Legend or Grid Displayed

Orientation

Select portrait or landscape orientation for
the graph by clicking the appropriate button.

9 - 15

EZ IQ Operating Manual

9.10 Change Data Graph Properties
Whenever a chromatogram or trace is displayed in the chromatogram window, it
uses the display settings contained in the Data Graph Properties. To change theData Graph Properties,
1 In the chromatogram window, right-click and select Properties.
2 Select the tab for the properties to view or change, as shown in Table 9-5.
Table 9-5 Properties Tab Parameters

Trace Setup

Add or remove traces, set legends, set scaling.

Axis Setup

Add a graph title, change data range, set margins andorientation, turn on and off legends.

Appearance

Set color schemes, line styles, and fonts.

9.11 Set Up a Trace
The Trace Setup tab, see Figure 9-13, allows the addition and removal of traces
as well as setting scaling options for the traces. Each row in the spreadsheet
represents one of the traces currently in the chromatogram window. The details of
the highlighted trace appear in the trace properties boxes in the bottom of thewindow where they can be viewed or changed.

IPN 074-538-P1A

Figure 9-13 Trace Setup Tab

9 - 16

EZ IQ Operating Manual

Table 9-6 Trace Setup Parameters

Show

Select this box to display the trace in thechromatogram window. Clear this box toremove the trace from the chromatogram
window. This is a convenient way totemporarily remove a trace from the viewing
window.

Lgnd

NOTE: If the Legend in the Axis Setup
window is not turned on, then thisbox will have no effect.

IPN 074-538-P1A

Select this box to show the legend for the
trace. The legend appears in the upper rightcorner of the window and displays the nameof the trace. Clear this box to remove thelegend for this trace from the chromatogram
window. Setup for the appearance of thelegend (color, etc.) can be accessed in the
Appearance tab for the graph item.
Data SourceEnter the name of the file from which to get
the trace. Or, click the File icon adjacent to
the field and select a data source. The data
source can be a chromatogram or it can bea stored profile such as temperature or flow
program.

Current Data

Selects a trace from the current
chromatography data.

Open Data

Selects a stored data file from which a tracefor display can be selected.

Current Method

Selects a trace from the current method (ifavailable). For example, temperature
profiles for instrument control.

Trace

Select the channel to be displayed.

9 - 17

EZ IQ Operating Manual
Table 9-6 Trace Setup Parameters (continued)

Scale toSelect one of the following scaling options.

Trace x

Scales to another trace in the window.

Autoscale to largest peak

Scales such that the largest peak is onscale.

Autoscale to 2nd largest peak

Scales such that the second largest peak is
on scale.

Autoscale to 3rd largest peak

Scales such that the third largest peak is onscale.

User Defined

Allows entering a value for Y maximum andminimum.

Normalized

Allows normalization of one trace to fit on thegraph.

Y Min

If User Defined scale is selected, enter aminimum value for the Y-axis.

Y Max

If User Defined scale is selected, enter amaximum value for the Y-axis.

Units

Select the units for display.

X OffsetEnter a value in units for offset of the X-axis.

Y OffsetEnter a value in units for offset of the Y-axis.

Y ScaleEnter a multiplier that will be applied to theentire trace.

9 - 18

Annotations

Click to display the trace annotations
window.

Hide Details

Click to hide the current trace details anddisplay only the spreadsheet.

Reset Scaling

Click to reset the scaling values to their
original values.

IPN 074-538-P1A

NOTE: Set the X-axis range by a right-clickthen selecting Axis Setup.

EZ IQ Operating Manual

9.12 Remove a Trace
If there are multiple traces in the chromatogram window, the user can remove one
or more of them by right-clicking anywhere within the window and selecting
Properties…. A spreadsheet will appear with the currently displayed traces listed.
To completely remove a trace from the chromatogram window, select the row byclicking on the number, then press the Delete key, or click Edit/Delete. To
temporarily remove the trace from the window, clear the checkbox in the Show
column. Click OK to return to the chromatogram window.

9.13 Set Limits for X-Axis and Y-Axis
Occasionally, an absolute range for either the X-Axis or Y-Axis, or both may bedesired. To set limits for the X-Axis and Y-Axis:

1 In the chromatogram window, right-click and select Properties. The Data
Graph Properties window is displayed. See Figure 9-14.

2 To set Y-Axis minimun and maximum values, select the Trace Setup tab. To set
an absolute voltage range for all chromatograms, use the User-Defined option
for the Scale To field. Then, enter a Y-Min (minimum Y-Axis value) and Y-Max(maximum Y-Axis value) for each chromatogram. To display all chromatograms
using this same voltage scale, enter the same values for all chromatograms.

IPN 074-538-P1A

Figure 9-14 Data Graph Properties Trace Setup Tab

9 - 19

EZ IQ Operating Manual

3 To set absolute ranges for the trace, select the Axis Setup tab, see Figure9-15. Click X-Axis, to set the range for the X-Axis. Click Autoscale to set the
X-Axis range automatically to the range of the longest chromatogram (thedefault selection), or click Use this range to enter an absolute range inminutes. The Get Current Axis Limits button brings in the X-Axis range fromthe current chromatogram window. This is useful because it allows the use of
the zoom function to identify the desired region of the chromatogram andautomatically enter the range values.

IPN 074-538-P1A

Figure 9-15 Data Graph Properties Axis Setup Tab

4 Once an absolute range for one or both of these axes is set, the designatedchromatogram(s) will always be displayed in the chromatogram window using
these ranges until they are changed or reset.

5 To reset the scaling of all chromatograms to default values, click Reset Scaling
in the Trace Setup tab. Refer to Figure 9-14.

9 - 20EZ IQ Operating Manual

Chapter 10
Chromatogram Operations
10.1 About Chromatogram Operations
Chromatogram comparison and mathematical operations are accessible from thechromatogram window. Right-click in the chromatogram window and selectOperations. See Figure 10-1 and Table 10-1.

IPN 074-538-P1A

Figure 10-1 Operations

Table 10-1 Operation Actions

Operation

ActionMove Trace"Grab" and move a trace within the chromatogram window.

Stack Traces

Positions multiple traces with an offset.

Align

Adjusts a second chromatogram such that a peak (or point)
on one chromatogram will be aligned with a peak (or point)on the first chromatogram.

Stretch

Performs a two-point contraction or expansion ofchromatogram relative to another.

10 - 1

EZ IQ Operating Manual
Table 10-1 Operation Actions (continued)

ActionNomalize

Normalizes one or more chromatograms to the first
chromatogram, adjusting the heights such that the apex
height of a selected peak matches that of the peak selectedon the first trace.

SmoothPerforms a 9-point Savitsky-Golay smoothing operation ona selected trace.

1st Derivative

Calculates and displays a 1st derivative of a selected trace.

2nd Derivative

Calculates and displays a 2nd derivative of a selected trace.

Add

Adds two traces and displays the result.

Subtract

Subtracts two traces and displays the result.

Multiply

Multiplies one trace by another and displays the result.

DivideDivides one trace by another and displays the result.

IPN 074-538-P1A

Operation

10 - 2

EZ IQ Operating Manual

10.1.1 Move a Trace
To "grab" a trace and move it:

1 In the chromatogram window, right-click and select Operations >> Move
Trace. See Figure 10-2.
Figure 10-2 Move Trace Utility

2 The Operations: Move Trace window will appear in the the upper right cornerof the chromatogram window. Move the pointer over a trace until the pointerchanges to a pointer with a cross with arrows at the end. See Figure 10-3.

IPN 074-538-P1A

Figure 10-3 Move Trace

10 - 3

EZ IQ Operating Manual

3 Grab the trace by pressing the left mouse button and dragging the trace to a
new location. When the mouse button is released, the trace will be placed at
the pointer location.

4 Move Trace will appear at the upper right corner of the window. Additional
traces can be moved. When finished, right-click and select Operations >>Move Trace again to turn off the move trace operation.

10.1.2 Stack Traces
To change the X-axis and Y-Axis offset for a trace:

1 In the chromatogram window, right-click and select Operations >> Stack
Traces....

2 Enter a new X-Axis and Y-Axis offset, and click OK. The offset will be applied
to additional traces displayed in the chromatogram window. See Figure 10-4
and Figure 10-5.

Figure 10-5 Chromatograms After Stacking

10 - 4

IPN 074-538-P1A

Figure 10-4 Chromatograms Before Stacking

EZ IQ Operating Manual

10.1.2.1 To Remove Offsets

1 In the chromatogram window, right-click and select Properties.
2 Select the Trace Setup tab, then scroll to the right to the X-Axis and Y-Axis
offset columns when the settings can be modified or deleted. See Figure 10-6.
Figure 10-6 Remove Offsets

3 Click Reset Scaling to restore all settings to their original values. Or, the Stack

IPN 074-538-P1A

command can be used again by entering "0" for both stack parameters.

10 - 5

EZ IQ Operating Manual

10.1.3 To Align Two Traces
To align one chromatogram to another:1 In the chromatogram window, right-click and select Operations >> Align. Clicka peak on the first chromatogram to align to, then click on a peak on the second
chromatogram to align it to the first peak. The selected peak in the second
chromatogram will be adjusted such that it will be aligned with the selectedpeak in the first chromatogram.

2 To remove the alignment, right-click and select Properties to view the tracespreadsheet. Select the Trace Setup tab, then scroll to the right to the X-Axis
and Y-Axis offset columns to delete or change these settings. Click Reset
Scaling to restore the original settings. See Figure 10-7 and Figure 10-8.
Figure 10-7 Chromatograms Before Alignment

IPN 074-538-P1A

Figure 10-8 First Peak of Top Chromatogram Aligned to First Peak on Bottom Chromatogram

10 - 6

EZ IQ Operating Manual

10.1.4 Stretch a Chromatogram
Stretch allows a two-point contraction or expansion of chromatograms relative toanother. To stretch a chromatogram:

1 In the chromatogram window, right-click and select Operations >> Stretch.
2 Select peaks on the first chromatogram to which the second will be stretched(or contracted).

3 Select two peaks on the second chromatogram. The chromatogram between
these two peaks will be stretched or contracted to fit the two peaks specified on
the original chromatogram.
10.1.4.1 To Undo the Stretch,

1 In the chromatogram window, right-click and select Properties. Select theTrace Setup tab, then scroll to the right to the X-axis and Y-axis offset columns
where the stretched or contracted settings can be changed or deleted. Click the
Reset Scaling button to restore all settings to their original values. See Figure
10-9 and Figure 10-10.

IPN 074-538-P1A

Figure 10-9 Chromatograms Before Stretching

Figure 10-10 Bottom Chromatogram Stretched Relative to Top Chromatogram

10 - 7

EZ IQ Operating Manual

10.1.5 Normalize Traces
Normalize Traces will normalize one or more chromatograms to the first
chromatogram, adjusting the heights such that the apex height of a selected peak
matches that of the peak selected on the first trace. Once Normalize Traces isselected, the user will be prompted to select the start and the apex of a peak in thefirst trace. Then a prompt will appear to click on the start and apex of a peak in thesecond trace for normalization.
To undo the normalization, right-click and select Properties to view the tracespreadsheet. Select the Trace Setup tab, then scroll to the right to the X-Axis andY-Axis offset columns where these settings can be deleted or changed. Click Reset
Scaling to restore all settings to their original values. See .Figure 10-11 and Figure
10-12
Figure 10-11 Chromatograms Before Normalization

IPN 074-538-P1A

Figure 10-12 After Normalization

10 - 8

EZ IQ Operating Manual

10.2 Perform Mathematical Operations on Traces
Mathematical operations on traces can be performed within the chromatogram
window. To perform a mathematical operation on a trace:

1 In the chromatogram window, right-click and select Operations, then select theoperation to perform. See Figure 10-13.

2 Follow the instructions displayed to perform the operation. The result of theoperation will appear in the window.

IPN 074-538-P1A

Figure 10-13 Mathematical Operations

10 - 9

EZ IQ Operating Manual

10.2.1 Smoothing
To perform a 9-point Savitsky-Golay smoothing operation on a selected data file:

1 In the chromatogram window, right-click and select Operations >> Smooth. A
Click on trace prompt will be displayed

2 Click on the chromatogram to be smoothed. The resultant trace will appear in
the window. See Figure 10-14 and Figure 10-15.
Figure 10-14 Chromatogram Before Smoothing

IPN 074-538-P1A

Figure 10-15 Smoothed Result Trace is Displayed with Original Trace

10 - 10

EZ IQ Operating Manual

10.2.2 Calculate Derivatives
To calculate and display the 1st or 2nd derivative of a chromatogram:

1 In the chromatogram, right-click and select Operations >> 1st Derivative or
Operations >> 2nd Derivative. A Click on trace window will be displayed.

2 Click on the chromatogram to perform the operation. The resultant trace willappear in the window. See Figure 10-16, Figure 10-17 and Figure 10-18.
Figure 10-16 Trace Before 1st Derivative

IPN 074-538-P1A

Figure 10-17 1st Derivative Trace Displayed with Original Trace

10 - 11

EZ IQ Operating Manual
Figure 10-18 2nd Derivative Displayed with Original Trace

10.2.3 Add Two Traces
To add two traces to a chromatogram window:

1 In the chromatogram window, right-click and select Operations >> Add.
2 Click on first trace to select it.
3 Click on the second trace, which will be added to the first trace. The resulting
trace will appear in the window. See Figure 10-19.
NOTE: For this operation to be valid, both traces must have the same
sampling frequency.

IPN 074-538-P1A

Figure 10-19 Adding Two Traces

10 - 12

EZ IQ Operating Manual

10.2.4 Subtract Two Traces
To subtract two traces:

1 In the chromatogram window, right-click and select Operations >> Subtract.
2 Click on the first trace to select it.
3 Click on the second trace, which will be subtracted from the first trace. The
resultant trace will appear in the window. See Figure 10-20.
NOTE: For this operation to be valid, both chromatograms must have the same
sampling frequency.

IPN 074-538-P1A

Figure 10-20 Subtract Two Chromatograms

10 - 13

EZ IQ Operating Manual

10.2.5 Multiply Two Chromatograms
To multiply two chromatograms:

1 In the chromatogram window, right-click and select Operation >> Multiply.
2 Click on the first chromatogram to select it.
3 Click on the second chromatogram, which will be multiplied by the first
chromatogram. The resultantchromatogram will appear in the window.
NOTE: For the multiply operation, the units of the resultant chromatogram are<chromatogram1 units> x <chromatogram 2 units>. See Figure 10-21.

IPN 074-538-P1A

Figure 10-21 Multiple Two Chromatograms

10 - 14

EZ IQ Operating Manual

10.3 Utilities
The Utilities menu in the chromatogram window provides access to commands forsaving, copying, or printing the current chromatogram window. To access theUtilities menu, right-click in the chromatogram window and select Utilities. See
Figure 10-22 and Table 10-2.
Figure 10-22 Utilties Parameters

IPN 074-538-P1A

Table 10-2 Utilities

Utility

ActionPrint

Sends the current chromatogram view to the printer.

Copy to Clipboard

Copies the contents of the chromatogram window to the
clipboard.

Save Trace...

Prompts to click on a trace then opens the save data filewindow.

10.3.1 Print
This command sends the current chromatogram window view to the printer.

10.3.2 Copy to Clipboard
To copy the contents of the chromatogram window to the clipboard, right-click andselect Utilities >> Copy to Clipboard.
This command copies the current chromatogram window to the clipboard as ametafile. From here, view can be pasted into a word processing document or otherapplication that supports the clipboard.
NOTE: To paste into Microsoft® Word, click Edit >> Paste >> Special >> Picture.

10 - 15

EZ IQ Operating Manual

10.3.3 Save Trace
Use this utility to save a trace as a data file.

1 From the Utilities menu, click Save Trace.
2 Click on the trace to save.
3 In the Save Data File As window, browse to the location for saving the file and

IPN 074-538-P1A

enter a name for the file.

10 - 16

EZ IQ Operating Manual

Chapter 11
Graphical Programming
11.1 About Graphical Method Programming
The Graphical Programming menu is used to add timed events and set up othermethod parameters graphically by clicking on the displayed chromatogram. These
commands are also available from the Graphical Programming Toolbar, which isdisplayed by default at the bottom of the Instrument Window. To turn on theGraphical Programming Toolbar,

1 From the menu, click View >> Preferences.
2 Click Int Events and then click Toolbar. See Figure 11-1 and Table 11-1.
Figure 11-1 Int Events Tool Bar

Parameters that can be set using graphical programming include:

IPN 074-538-P1A

Table 11-1 Graphical Programming Parameters

Command

ActionWidth

Inserts a Width event at the point on thechromatogram.

ThresholdInserts a Threshold event at the point on thechromatogram.

Shoulder Sensitivity

Inserts a Shoulder Sensitivity event at the point on thechromatogram.

Integration Off

Turns off integration at the point on the chromatogram.

Valley to Valley

Turns on valley to valley baseline detection.

Horizontal Baseline

Forces a horizontal baseline from the point on thechromatogram.

Backward Horizontal Baseline

Forces a backward horizontal baseline from the pointon the chromatogram.

Lowest Point Horizontal Baseline Forces a horizontal baseline at the next lowest point.
Tangent Skim

Forces a tangent skim.

Front Tangent Skim

Forces a front tangent skim.

Minimum Area

Set a minimum area for peak detection.

Negative Peak

Turn on negative peak detection.

11 - 1

EZ IQ Operating Manual
Table 11-1 Graphical Programming Parameters (continued)

ActionDisable Peak End Detection

Disables the end of peak detection.

Reassign Peak

Designates a different peak as the calibrated peak.

Manual Baseline

Manually define a baseline.

Manual Peak

Manually define the beginning and end of a peak.

Split Peak

Force a perpendicular to split a peak.

Force Peak Start

Force the start of a peak.

Force Peak Stop

Force the end of a peak.

Move Baseline

Manually move a baseline.

Reset Baseline

Force a baseline to the point.

Reset Baseline at Valley

Reset the baseline to the next valley.

Adjust Retention Time Window

Adjusts the retention time window.

Adjust Group Range

Adjust the group range.

Define Single PeakDefine a single peak and add it to the peak calibrationtable.

Define Peaks

Define multiple peaks and add them to the peakcalibration table.

Define Groups

Define groups and add them to the group calibrationtable.

Suggest Sampling Frequency

Suggest a sampling frequency for the chromatogram.

IPN 074-538-P1A

Command

11 - 2

EZ IQ Operating Manual

Chapter 12
Data Acquisition and Control
12.1 Data Acquisition and Control
Commands that are available from the Control menu are related to dataacquisition and control of the instrument. In general, there are two ways to acquiredata:Single run acquisition, where data is acquired for a single injection.



Sequence acquisition, where data is acquired automatically for a series of runs
using a pre-programmed sequence that defines the number of injections,
methods, file names, and calibration.

Additional control menu items will appear depending on the features supported by
the instrument configured.

12.2 Single Run Acquisition
There are two ways to acquire data. One way is with a sequence (for multiple runs),
and the other way is to make a single run. To make a single data acquisition run,specify the method to be used for analysis, and a filename for data storage.

IPN 074-538-P1A

NOTE: In order to use a method for data acquisition, its Instrument Setup shouldhave the acquisition channel turned On, and a sampling rate and run time
designated.
To make a single run, click the Single Run icon
or click Control >> Single
Run. The Single Run Acquisition window is displayed, see Figure 12-1 and Table
12-1.

12 - 1

EZ IQ Operating Manual
Figure 12-1 Single Run Acquisition Window

12 - 2Run information

Used to specify various files for the run.

Sample ID

Enter a Sample ID for the run. This cancontain text and numbers, and is saved withthe data file. Click the arrow to select from anumber of predefined ID’s.

MethodEnter the name of the method to be used fordata acquisition and processing. Include theentire path name if the method is not in thedefault method directory. Select the methodfrom a list of available methods by clicking
the File icon adjacent to the field.

Data PathEnter a path name where the data acquired
for this run will be stored. Click the File icon
to select a path from a listing of available
paths.

Data FileEnter a filename for the data to be saved on
disk. Select from one of the predefinednames by clicking the arrow button adjacentto the field. It is not possible to use an
existing filename, unless the file exists inlocated in a directory whose path contains
the term "public". For example, if the data
files are saved in a directory entitled
C:\Public\Data, the files saved in thisdirectory can be overwritten. The software
automatically appends a .dat fileextension.

IPN 074-538-P1A

Table 12-1 Single Run Acquisition Parameters

EZ IQ Operating Manual

IPN 074-538-P1A

Table 12-1 Single Run Acquisition Parameters (continued)

Number of reps

Enter the number of runs to conduct. The
runs will automatically proceed without
review until completed, incrementing eachfilename as designated. If the sequence of
single runs is aborted, and the single
acquisition is repeated without changing any
parameter, the run number will start with thenext number as if the sequence was notaborted. For example, setting 4 runs withstarting run number of 101, then aborting
during run 102, upon restarting the next run
number will be 105. If the Sample ID is also
incremented, it will increment in parallel.

Print Method Report

When this box is selected, the method report(or reports) will be printed at the end of therun.

Amount Values

Enter values that affect how theconcentrations are calculated. When making
a single data acquisition prior to calibrating
the method, leave these values at theirdefault.

Sample amountThe Sample amount value is used as adivisor during calculation of concentrations.
It is intended to compensate for differences
between samples due to weight and whenpercentages of the total sample are being
calculated rather than the amount detectedin an injection.

Internal standard amountFor calibration runs, the Internal Standard
Amount is acquired from the method Peak
Table. For unknown runs, enter the amountof the Internal Standard in an unknown
sample.

Multiplication factors

Enter one to three multiplication factors to be
used for this run. All quantitated peaks will
be multiplied by these factors.

Dilution factors

Enter one to three dilution factors to be used
for this run. All quantitated peaks will be
divided by these factors.

12 - 3

EZ IQ Operating Manual
Table 12-1 Single Run Acquisition Parameters (continued)

Calibrate

Select this box if the sample is to be acalibration sample. Once this box isselected, the following fields and options will
be available.

Calibration level

Enter the number of the calibration levels
represented by this calibration standard. If
this is a single level calibration, enter 1.

Clear all calibration

Select this box to clear all existing calibration
factors from the method before running thesample.

Clear calibration for level

Select this box to clear the existing response
factors for a specific level before running thesample.

Print calibration report

Select this box to print a calibration report
after running the sample.

Clear replicates

Select this box to clear all existing replicatesfrom the existing calibration level before
running the sample.

Average replicates

Select this box to average the replicates forthis calibration level.

Begin run

By default, a run will start immediately. Click

When the Single Acquisition Run window
is completed, click Start to begin theacquisition. The current data will appear in
the chromatogram window as it is acquired
and will be stored on disk. At the end of therun, the chromatogram will be analyzed
according to the method parameters, and, if
specified, a report will be generated. If thesample analysis is not displayed at the endof the acquisition, click Analyze to view theresults.

12 - 4

IPN 074-538-P1A

to open the Schedule Run window toenter or select the time to start thesequence.

EZ IQ Operating Manual

12.3 Run a Sequence
Once a sequence has been created and saved, it can be used to acquire andprocess data. To start a sequence acquisition:

1 From the Instrument Window, click the Sequence Run icon

or from theControl menu, click Sequence Run.... The Run Sequence window isdisplayed. See Figure 12-2 and Table 12-2.

2 Enter or select a sequence to use, set a run range, mode, printing options andreview options, then click Start.
Figure 12-2 Run Sequence Window

IPN 074-538-P1A

Table 12-2 Run Sequence Parameters

Sequence information

Enter the Sequence Name to be used, or
select the sequence file from a list ofavailable sequence files by clicking the Fileicon.

Run range

Select the range of the sequence to be run.

All

Click All to execute all runs in the sequence.

Selection

If a series of runs in the sequencespreadsheet has been selected(highlighted), click Selection to run only thehighlighted runs.

RangeEnter a range of runs to be executed. For
example, an entry of 4 - 6 will execute runs
4, 5, and 6 of the sequence. An entry of 4
executes the fourth run through to the end of
the sequence.

12 - 5

EZ IQ Operating Manual
Table 12-2 Run Sequence Parameters (continued)

Select the manner by which to handle
autosampler dual towers (if any), processing
mode, and bracketed calibration (if used).
Tower

If the instrument is configured for Dual
Tower, select the tower mode to be used forthe sequence run. Selections include Dual,
Front, and Rear.

Processing mode

Select a mode for reprocessing the data.
Options will vary depending on the
instrument configuration. If the instrument
does not support this feature, Processing
mode will be grayed out. For certain
instruments, Overlap Sample Prep mode
will be available. See About Overlap Sample
Prep for information and restrictions forusing this mode.

Bracketing

Select the type of bracketing to perform.
(See Bracketed Calibrations for details.)

None

Select this to NOT bracket calibrations.

Standard

Select this to perform the standard mode ofbracketing calibrations.

Std. w/Clear Calib

Select this to perform the standard mode ofbracketing calibrations, clearing thecalibration before the start of eachcalibration set.

Sequence

Select this to perform the sequence mode ofbracketing calibrations.

Seq. w/ Back Calc

Select this to perform the sequence mode ofbracketing calibrations and back-calculate
calibration runs.

Review

12 - 6

Results Review

Select this box to pause the sequence
between runs in order to review results.

Calibration Review

Select this box to pause the sequence after
each calibration set, where a calibration set
is defined as one or more calibration runsthat occur in a sequence.

IPN 074-538-P1A

Mode

EZ IQ Operating Manual
Table 12-2 Run Sequence Parameters (continued)

Printing
Print method reports

Select this box to print a custom report,
defined in the method, for each run of thesequence.

Print sequence reports

Select this box to print sequence reports.

Begin run

NOTE: By default, a run will start
immediately.
To schedule the start of the sequence for alater time or date, click theicon to open
the Schedule Run window where the time tostart the sequence can be entered or
selected.

Email Recipient(s)Use this field to enable e-mail notification to
be sent to a designated address (entered in
the To: field).
Select the On Start box to send e-mailnotification when the sequence starts.
Select the On Stop or Error box to sende-mail notification when the sequence stops
or if an error occurs.

IPN 074-538-P1A

When the window is completed, click Start
to initiate the sequence acquisition. The data
is displayed in real time in the chromatogram
window(s), if the current data is selected forviewing.

12 - 7

EZ IQ Operating Manual

12.4 Stop a Run in Progress
NOTE: STOP cannot be accessed by anyone other than the instrument controller.
To stop data acquisition during a run:

1 Click the STOP icon, on the command toolbar, when the run is in progress, or
click Control >> Stop Run. The Stop Run window is displayed. See Figure12-3 and Table 12-3.
NOTE: When using the STOP icon, press the left mouse button down until the
STOP icon changes appearance before releasing the mouse button.

2 Select the desired manner to stop the run.
Figure 12-3 Stop Run Window

Stop current run only

Select this to end the run currently inprogress. If the run is a part of a currently
queued sequence, the sequence willcontinue with the next run.

Stop current run and sequence run

Stops the run currently in progress, andterminates it’s sequence. Other queued
items will proceed.

Stop sequence after current run completes Aborts the sequence after the current run iscompleted.
Stop all run queue items submitted

12 - 8Stops the run currently in progress, andterminates all the items in the queue thatwere submitted by the current user. Queueitems submitted by other users will be
unaffected.

IPN 074-538-P1A

Table 12-3 Stop Run Parameters

EZ IQ Operating Manual
Table 12-3 Stop Run Parameters (continued)

Stop all run queue items

This selection stops the run currently inprogress, and terminates all items in the runqueue.
NOTE: When a run is stopped, the data up
to that point is saved in the data file.
However, no analysis of the data
will be performed. To produce areport or view results from a runthat was stopped, Analyze the data
file.

12.5 Extend Run
NOTE: If the current user is not the instrument controller, or the current user isusing an instrument offline, the current user does not have access toExtend Run.
While a run is in progress, data acquisition can be extended beyond the designated
run time by:

1 Click Control >> Extend Run.
2 The Extend Run window is displayed. See Figure 12-4.
3 Enter the number of minutes to extend the run, then click OK.

IPN 074-538-P1A

Figure 12-4 Extend Run Window

12 - 9

EZ IQ Operating Manual

IPN 074-538-P1A

This page is intentionally blank.

12 - 10

EZ IQ Operating Manual

Chapter 13
Tutorial
This chapter guides the user through the basics of using EZ IQ. Follow the steps
to set up a method and acquire a data file, then optimize the method for integrationand set up calibration. Use the Tutorial files provided with the software to learn thesoftware and become comfortable with its use. Details on data file structure,application window features, how to open and save files are located in Chapter 4,
Program Architecture and Data Structure, and Chapter 5, Opening and Saving
Files.
NOTE: This Tutorial assumes:That EZ IQ is installed with all instruments installed and properly
configuredThe PC running EZ IQ is connected to a 3000 Micro GC instrument via
the TCP/IP connection

The 3000 Micro GC enhanced driver is loaded for the EZ IQ
configuration. See Chapter 8, Acquisition Parameter Setup, for theprocedure to load a 3000 Micro GC configuration using EZ IQ.

13.1 Tutorial Overview
The following is a quick overview of the tutorial contents. For best results, follow all
steps in the Tutorial, in the order presented.

IPN 074-538-P1A

1 Create a Data Acquisition Method

Set up acquisition run time and sampling rate

Save the methodRun a preliminary sample

Set integration parameters graphically

2 Create a Single-Level CalibrationName calibrated peaks

Run a single-level calibration

3 Create and Run a Sample Sequence
4 Using Tutorial Files

Review a multi-level calibration



Explore a peak tableExamine a custom reportChange integration parameters

13 - 1

EZ IQ Operating Manual

13.2 Instrument and Method Wizards
Instrument and Method Wizards make it easy to locate and step through thewindows necessary to create or modify methods. To create Tutorial methods, use
the wizard buttons to display the window associated with a Tutorial step.
The Method Wizard is started from the Instrument Wizard by clicking Create or
Modify a Method. See Figure 13-1.
Figure 13-1 Instrument Wizard

The Method Wizard window will display. See Figure 13-2.
IPN 074-538-P1A

Figure 13-2 Method Wizard

Click Create a New Method to start creating the tutorial method.

13 - 2

EZ IQ Operating Manual

When Create a New Method is selected, the Method Wizard sets up icons in theapplication window that allow the user to "step" through all windows of method
generation. A Save icon is also provided. See Figure 13-3.
Figure 13-3 Step and Save Icons

13.3 Create a Data Acquisition Method
The first step toward acquiring a data file is to create a data acquisition method.
Ensure that the method will acquire data with a long enough run time for the last
peak to elute, using the default data acquisition sampling rate (which is adequate
for most samples except for fast capillary runs).
NOTE: Skip this step and use data and method files provided if there is no accessto an instrument.

13.3.1 Acquisition Setup
To set acquisition run time and sampling rate, go to the Instrument Setup window.
See Figure 13-4. This is the first window displayed when using the Method Wizard.
Or, click Method >> Instrument Setup, or click the Instrument Setup icon.
NOTE: The tabs and contents of the Instrument Setup area will vary dependingon the type of instrument and its configured modules.

IPN 074-538-P1A

Figure 13-4 Instrument Setup Window

13 - 3

EZ IQ Operating Manual

Select the Trigger tab and select the Type for the type of remote start (if any)
installed on the instrument. The trigger type for each instrument is set up duringconfiguration. If no trigger is being used, select None for Type. See Figure 13-5
and Table 13-1.
Figure 13-5 Trigger Tab

External

Data sampling starts when externallytriggered.

ManualUser must start the data sampling.

None

Sampling of data starts immediately whenStart is clicked.

When the acquisition setup information is complete, click the X box in the upper
right hand corner of the tab window to exit the window.

13 - 4

IPN 074-538-P1A

Table 13-1 Trigger Tab Parameters

EZ IQ Operating Manual

13.3.2 Save the Method
Once the acquisition parameters are set, save the method. Click File >> Method>> Save As…. The Save Method File As window will appear. See Figure 13-6.

IPN 074-538-P1A

Figure 13-6 Save Method File As Window

Select the folder in which to save the method file. In the File name field, enter
Test.met as the filename for saving the method.

13 - 5

EZ IQ Operating Manual

13.3.3 Downloading a Method
When a new method is created, or when a method is opened, it must be
downloaded to the 3000 Micro GC to make it available on the instrument.
Click Control >> Download Method. See Figure 13-7.

IPN 074-538-P1A

Figure 13-7 Downloading a Method

13 - 6

EZ IQ Operating Manual

13.3.4 Run a Preliminary Sample
The data acquisition method (Test.met) will be used to make the first dataacquisition run. To aid in later steps of the Tutorial, it is best to run a standard
sample for the first acquisition run.

1 To start the run, click Control >> Single Run. Refer to Figure 13-7. The Single
Run Acquisition window is displayed. See Figure 13-8.
Figure 13-8 Single Run Acquisition Window

2 Enter Test in the Sample ID field.
3 In the Method field, either enter Test.met, along with its path, or select theTest.met file from a file list by clicking the Open File icon adjacent to the field.

4 Enter a path for storage of data files in the Data Path field, or select a path froma list presented by clicking the Open File icon adjacent to the field.
IPN 074-538-P1A

5 Enter Test.dat as the name for storing data in the Data File field. A unique filename must be entered in this field. If this tutorial has been performed
previously, any saved files will need to be deleted or moved to a different
directory.

6 Click Start and inject the standard sample. As it is acquired, data will be
displayed in the chromatogram window.

13 - 7

EZ IQ Operating Manual

13.3.5 Set Integration Parameters Graphically
EZ IQ uses default integration parameters that are appropriate for most simple
chromatography. However, certain peaks may require special integration
treatment. Such special integration treatments are entered into the method as
Integration Timed Events. These events can be placed at the beginning of the run
to apply to all peaks, or they can be inserted at a certain place in the chromatogram
such that only some peaks are affected. Follow the steps below to add the timed
event to turn off integration to the method.
NOTE: This step can be performed using the Multi calibration.dat file provided
with the software.

1 At this point, the recently acquired chromatogram will be displayed in thechromatogram window. If it is not, click File >> Data >> Open... and then selectthe data file from the list displayed. Alternatively, one of the files supplied with
EZ IQ can be selected.

2 Click Analyze to integrate the chromatogram and display the baselines.
NOTE: If an icon’s function is unknown, hover the pointer over the button anda Tooltip will display showing the icon’s name or function.

3 To add the Integration Off timed event, click the Int Off button on the Int.
Event Toolbar. Click on a part of the chromatogram to turn integration off at thatpoint. This is the Start Time. Click on the chromatogram again at the point
where integration will be turned on again. This is the Stop Time. An
Integration Off window will appear. See Figure 13-9.

IPN 074-538-P1A

Figure 13-9 Integration Off

The integration will be turned off between the Start Time and the Stop Time. The
Value is set at zero, as no numeric value is required for this event. Click Analyze
Now to add the event to the method and re-integrate the chromatogram. (Clicking
Add to Table will add the event to the integration timed events table without
re-integration.)

13 - 8

EZ IQ Operating Manual

Select Insert into Integration Events Table and the event will be added to theIntegration Events Table of the method (where it will be used on all chromatograms
analyzed using this method). Select Insert into Manual Integration Fixes Table
and the event will be added to the Manual Integration Fixes Table of the present
chromatogram (where it will be applied only to this chromatogram).
The chromatogram will be re-drawn using the new integration event. Notice that the
selected area has no baselines drawn because the integration has been turned off
for these peaks.

4 The integration event is put into the Integration Timed Events table. To view thistable, click Integration Events from the Navigation panel or click Method >>Integration Events... The integration timed events table will appear. Notice theIntegration Off timed event has been added to the table.

5 To remove the Integration Off event from the method, click the Integration Off
event name or the row number, then press the Delete key on the keyboard. The
event can also be deleted using the Edit >> Cut command. The event can bere-inserted using the Edit >> Paste command. To temporarily view the effect ofremoving an event without actually removing it from the table, select the checkbox adjacent to the event to clear it. To re-select the event, select the check box
once again.

6 When finished with the Integration Events table, close it to return to the

IPN 074-538-P1A

chromatogram.

13 - 9

EZ IQ Operating Manual

13.3.6 Create a Calibration
For peak quantitation (calculation of results based on the running of standards), themethod for calibration must be set up. For further details on how to set up multiple
level calibrations, refer to the Calibration in the online help file. For this tutorial, theuser will set up a single level of calibration.
Setting up calibration involves the following steps.


Identify the Calibrated Peaks and enter known standard amounts in the methodRun the standard sample(s)Review the calibration curve

The easiest way to enter calibration peak data is to run the standard sample first,
then use the stored data file to graphically define the calibration peaks. If the user
has been following this Tutorial, there should already be a standard sample saved.
If not, the user can either run a standard sample using the steps shown in section
13.3.4, Run a Preliminary Sample, on page 13-7, or the user may select one of thedata files provided.

1 Open the stored data file by clicking File >> Data >> Open... Select thestandard data file from the list, or select one of the supplied data files. Once thefile is displayed in the current data chromatogram window, click Analyze tointegrate the chromatogram and show the baselines.

2 Click the Define Single Peak button from the Int Event toolbar. The DefineSingle Peak window will display for the first detected peak in thechromatogram. See Figure 13-10 and Table 13-2.

IPN 074-538-P1A

Figure 13-10 Define Single Peak Window

13 - 10

EZ IQ Operating Manual

Table 13-2 Define Single Peak Window Parameters

Retention TimeThe retention time of the first detected peak
will appear. To add this peak to the peaktable, populate the window for this peak. To
skip this peak, click Next. To move to a
specific peak in the chromatogram, click on
the specific peak. The retention time shownin the window will change to reflect the
selected peak.

Peak NameEnter the name of the compound in this field.

Conc Level

Concentration Level 1 is shown. Enter theconcentration of this component in the field
to the right of the Conc. Level.
NOTE: The concentrations of calibration
gas components are normallyprovided by the gas supplier, forinstance as a label attached to thegas cylinder.
NOTE: For setting up more than one level
for this compound, enter 2 for theConcentration Level and enter
the concentration for that level.
Continue to enter concentrationlevels until all desired number ofcalibration levels are completed.
Enter the units to be used when displaying
the results.

ISTD ID #If performing an internal standard
calibration, enter the ID # for the internal
standard peak for this compound. This is thepeak ID number from the peak table. If the ID# is unknown, it can be added in the peaktable later.

Ref ID #The Ref ID # corresponds to the peak ID # in
the peak table. Enter the peak ID # in the Ref
ID # field. If the peak ID # is unknown, it canbe added in the peak table later. Reference
peaks are used to locate calibrated peaks
when chromatographic conditions changesuch that retention times shift.

IPN 074-538-P1A

Units

13 - 11

EZ IQ Operating Manual
Table 13-2 Define Single Peak Window Parameters (continued)

Retention Time Window

Select the parameter to enter the retention
time window for this peak. The window isused for peak identification in case of slightdeviations from the expected retention time.

Relative

Click Relative to calculate the retention time
window based on a % of the expected
retention time of the peak. Enter the % touse for calculation of the window.

Absolute

Click Absolute to enter an absolute window
for the peak. Enter the value for the retention
time, in minutes.

3 Click Next to move to the next detected peak. Click Back to move to theprevious detected peak in the chromatogram. The current peak and total peaksin the chromatogram are displayed on the right of the window. When finishedadding peaks to the peak table, click Done. Each defined peak will become arow in the peak table. If peaks are already in the peak table, the recently definedpeaks will be added to those already present.

4 Once peaks have been defined, click Method >> Peaks/Groups... to display
the Peak/Group Tables window. See Figure 13-11. Click the Named Peaks
tab. Each peak defined will appear as a row in the Peak Table spreadsheet,along with its retention time and the other parameters entered.

IPN 074-538-P1A

Figure 13-11 Calibration Peak Table

Information in the table can be edited or changed by scrolling through the rows
and columns. Details on contents of the Peak Table are described in the
Calibration chapter of the online help file.

13 - 12

EZ IQ Operating Manual

5 Do not enter information in the other columns at this time. Close thePeak/Group Tables.
The method is now ready to be calibrated. Before calibrating, save the method.
Click File >> Method >> Save. (To save the method using a different name, click
File >> Method >> Save As.)

13.3.7 Calibrate Using a Stored Data FileIn order for the software to calculate concentrations for unknown samples, themethod must contain the areas generated for each component in the standard
sample. To store these areas into the method, either run the standard sample
again, designated as a calibration run, or calibrate the method using the stored
data file created earlier in this tutorial, using Analysis >> Single Level
Calibration. To use the stored data file to calibrate the method:

1 Click Analysis >> Analysis Single Level Calibration. A window will display
where file information for the calibration must be set. See Figure 13-12.

2 In the Sample ID field, enter sample identification information. If following thistutorial, enter Test.

IPN 074-538-P1A

Figure 13-12 Analysis/Single Level Calibration Window

3 In the Method field, type the data path name for the method to calibrate. Clickthe Open File icon and select the method file from the list. The tutorial method
is Test.met.

4 In the Data path field, type the data path name, or select it from the list using
the Open File icon.

5 Type the name of the calibration data file in the Data File field. Click the Open
File icon and select a saved filename—the name used in this tutorial isTest.dat.

6 Leave the Amount Values set to 1. For details on how these values are used,refer to the Calibration chapter in the online help file.

13 - 13

EZ IQ Operating Manual

7 Select the Calibrate checkbox and enter 1 for Calibration Level. Since thismethod is currently uncalibrated, it is unnecessary to select any of thecheckboxes dealing with calibrations or replicates. However, if unsure of themethod contents, select Clear all calibration before starting.

8 When the window is populated, click Start. When the analysis is complete, thechromatogram will be integrated, and the areas for the peaks identified as
calibration compounds will be entered into the method. Enter the concentrationof the calibration gas into corresponding Level field in the Peak / Group Tables
if not already populated.The calibration curves will be generated using theseareas. At this point, the method is calibrated for a single level, and it can now
be used to run and analyze samples with the calibration compounds in
unknown amounts.

13.3.8 Create and Run a Sample Sequence
If using an automatic stream selection valve to inject samples, the user must define
the samples to be injected and how they will be acquired and analyzed. This isperformed using a sample Sequence. A sample sequence can be used to acquire
both calibration and unknown samples. It can also be used to automaticallyre-analyze stored data files. Details on creating and using a sequence can be foundin the Sequence chapter of the online help file. In this part of the Tutorial a simple
sequence will be created and used to acquire a calibration sample and two or three
unknown samples.

1 To create a new sequence, click File >> Sequence >> Sequence Wizard. A
Sequence Wizard window will display. See Figure 13-13.

IPN 074-538-P1A

Figure 13-13 Sequence Wizard - Methods Window

13 - 14

EZ IQ Operating Manual

2 Enter the method to be used for the acquisition, or select it from a list ofavailable methods by clicking the File Open icon.

3 For Method, enter the method name (including path) for the method created inthis Tutorial. The user can select the entire path name by clicking the File icon
next to the field, and selecting the method name from the list displayed. If theuser is following this Tutorial, select Test.met as the method name. Leave theAmount Values at their default values.

4 Select For acquisition. The Sequence Wizard will prompt for information
required for data acquisition.

5 Click Next. The Sequence Wizard - Unknowns window will display. See
Figure 13-14.

IPN 074-538-P1A

Figure 13-14 Sequence Wizard - Unknowns Window

6 The Sample ID field is used to identify the samples. Click the blue arrow andselect the Line Number and Method Name. Each Sample ID will contain thesequence line number and the current method name.

7 In the Data path field, enter the path to where the data will be stored, or selectan existing path by clicking the File Open icon.

13 - 15

EZ IQ Operating Manual

8 For Data file, click the blue arrow and select Sample ID. The data files will be
named using the sample ID selected above. Using numbered identification
ensures the data filename for each run is unique, preventing errors that will
occur when trying to acquire data using an existing data filename. See Figure13-15.
Figure 13-15 Sequence Wizard - Unknowns Window

9 For Number of unknown runs in sequence, enter 3. Leave the other fields at

IPN 074-538-P1A

their default values.

13 - 16

EZ IQ Operating Manual

10 Click Next to continue.
Figure 13-16 Sequence Wizard - Calibration Window

11 In Figure 13-16, the Calibration ID is automatically set to the identification from

IPN 074-538-P1A

the previous window. Set the Number of calibration levels to 1, and leave thecalibration Repetitions per level at 1. Leave all other boxes cleared. Click
Next.

13 - 17

EZ IQ Operating Manual

12 Select Include unknown runs in summary report and Include calibration
runs in summary report. Do not check the other boxes. See Figure 13-17.
Figure 13-17 Sequence Wizard - Reports Window

13 Click Finish. A sequence table will appear, with the file and method names
specified. See Figure 13-18.

IPN 074-538-P1A

Figure 13-18 Sequence Table

14 At this point, the sequence is set up to run one calibration sample and three
unknown runs. Notice the Sample IDs and Filenames are numberedautomatically to prevent duplication. In order to run a calibration standard as
the first run, the the run must be designated as a calibration run. This was
performed automatically by the Sequence Wizard. Unknown runs always havea Level of 0. The information in the Run Type field may be abbreviated if there
is more than one run type designation. To view the possible Run Types, click
the arrow next to the run type. For details on each of these run types, refer tothe Sequence chapter in the online help file. Since the method created in thisTutorial is a single level calibration, only one calibration standard run isnecessary.

13 - 18

EZ IQ Operating Manual

15 To save the sample sequence file, click Save\Save Sequence to go to theSave Sequence window, or click File >> Sequence >> Save As. Select the
C:\datasystem\Sequence folder (where datasystem is the program
installation folder), then type the name Test or Test.seq for the sequence filename.
NOTE: By default, sequence files are saved with the .seq extension.

13.3.9 Run a Sequence
To acquire data using the sequence file just created, click Control >> Sequence
Run, or right-click in the Sequence table and select Run Sequence. A Run
Sequence window will display.
. See Figure 13-19.

IPN 074-538-P1A

Figure 13-19 Run Sequence Window

Enter the name of the sequence file by typing the name, along with path, in the
Sequence Name field. Alternatively, select it from a list of sequence files byclicking the Open File icon to the right of the Sequence Name field. Leave theother parameters as their defaults.
Prepare the automatic stream selection valve to inject a standard sample, followedby three unknown samples. When ready to inject the first sample, click Start. Whenthe sequence is completed, data files are acquired and saved for one standard andthree unknown runs, and a simple result report for each unknown sample and asummary report for the sequence are generated.
Because a sequence summary report has not yet been defined, do not select the
Print sequence reports box.

13 - 19

EZ IQ Operating Manual

13.4 Review Multi-level Calibration Curves
Once a method is calibrated, the calibration curves and associated data can beviewed using Review Calibration. To see a fully calibrated multilevel calibration,
use the Multi Calibration.met file provided with the software.

1 Open the Multi Calibration.met method file by clicking Open >> Open
Method. Select the Multi Calibration.met file. It will be located in the\EZIQ\Projects\Default\Method folder.

2 Once the Multi Calibration.met method has been opened, click Method >>Review Calibration. The window shown in Figure 13-20 is displayed.

The calibrated peaks in the method are listed in the peak list at the top rightcorner of the window. The calibration curve shown is for the highlighted peak.
View the other curves by highlighting their peak name. The top left portion of
the window contains a table that displays all the calibration information,
including areas used to create the current calibration curve.

3 The calibration curve fit type by default is Point-to-Point. To overlay a different
fit type, right-click anywhere in the calibration curve box and select Fit Type >>Linear. Notice the new linear calibration curve is overlaid on the Point-to-Point
curve. In the box at the right, the equations for the different fit types displayed
are shown, along with the Goodness of fit calculation.
NOTE: The r2 value is not calculated for the Point-to-Point curve since it is bydefinition a perfect fit to the data.
For additional details on using Review Calibration, refer to the Calibration
chapter in the online help file.

13 - 20

IPN 074-538-P1A

Figure 13-20 Review Peak Calibration

EZ IQ Operating Manual

13.5 Explore a Peak Table
Method calibration information is located in the Peak / Group Tables. In thissection, the user will use the method provided with the software to examine a Peak
Table and become familiar with a completed peak table.

1 Open the Multi Calibration.met method which is located in\EZIQ\Projects\Default\Method by clicking File >> Method. Select the Multi
Calibration.met method from the list.

2 Open the Multi Calibration.met method. View the Peak / Group Tables byclicking Method >> Peak/Groups. The following table will display. See Figure
13-21.
Figure 13-21 Peak Table

IPN 074-538-P1A

3 In the Named Peaks tab, a table containing all of the calibration information forthe calibrated peaks in this method is displayed. By scrolling to the right, manydifferent columns appear, each of which represent a parameter for thecalibration, such as Levels, which contain the calibration amounts for eachcompound at each level of calibration. Note that it is possible to customize thePeak Table such that only parameters needed for a given calibration aredisplayed. Details on what each column represents, along with how tocustomize the Peak Table, are available in the Calibration chapter in the online
help file.

13 - 21

EZ IQ Operating Manual

13.6 Examine a Custom Report
A complete suite of report templates are provided with the software that can beused without modification to generate reports. To see an example of one of thesereports, click Reports >> View >> External Standard. (Make sure the current
chromatogram has been analyzed first.) The External Standard Report will be
displayed. See Figure 13-22.

If the method contains no defined custom report, the system will use the standard
report formats to print reports.
The standard report templates can be modified, or entirely new reports can becreated using Custom Report. Both custom method reports and custom
Sequence reports can be created. These are described in detail in the Custom
Reporting chapter in the online help file.

13 - 22

IPN 074-538-P1A

Figure 13-22 External Standard Report

EZ IQ Operating Manual

To view the custom report in the Multi Calibration.met file, open the file if it is notalready open (click File >> Method >> Open, then select Multi Calibration.met
from the file list). Click Method >> Custom Report to access the method custom
report editor. The current method custom report will appear. See Figure 13-23.

IPN 074-538-P1A

Figure 13-23 Method Custom Report

Examine the custom report template by scrolling through it using the scroll bars.
Before attempting to edit or create a custom report, thoroughly review Custom
Reporting chapter in the online help file. To return to the method, close the Method
Custom Report window.

13 - 23

EZ IQ Operating Manual

13.7 Changing Integration Parameters
Another important aspect of using a computerized data system is the ability tocustomize the integration using Integration Timed Events. In this part of the
Tutorial, use the Multi Calibration.dat data file to become familiar with how toenter integration timed events into the method, and to view the effects of some ofthese events. Complete details on how each integration timed event functions are
given in the Calibration chapter in the online help file.

1 Open the Multi Calibration.dat data file by clicking File >> Data >> Open, andthen select the Multi Calibration.dat file from the \Data folder. Click Open with
Method to open the Multi Calibration.met method file that was used toacquire the data file.

2 Click Analysis >> Analyze to analyze the chromatogram and display thebaselines. See Figure 13-24.

NOTE: The vertical line pointer moves with the mouse. The retention time where
the pointer is located is shown at the top of the chromatogram window.

13 - 24

IPN 074-538-P1A

Figure 13-24 Analyzing Channel A

EZ IQ Operating Manual

3 Add the Valley to Valley timed event to integrate the cluster of 4 large peaks
with Valley to Valley baselines. To do this, right click on the chromatogram andthen select Graphical Programming >> Valley to Valley.

4 Then, click once before the first large peak, then click again just after the lastpeak. See Figure 13-25.
Figure 13-25 Valley To Valley Window

When the Valley to Valley window appears displaying the start and stop points forthe event, click Analyze Now and view the chromatogram. Notice the peaks within
the region of the event are now integrated using the valley-to-valley event, and thebaselines are adjusted accordingly. See Figure 13-26.

IPN 074-538-P1A

Figure 13-26 Chromatogram

13 - 25

EZ IQ Operating Manual

5 Click Method >> Integration Events. Note the addition of the Valley to Valley
event in the table. See Figure 13-27.
Figure 13-27 Integration Events Table

6 Remove the Valley to Valley event by selecting the checkbox next to it, thenpress Delete on the keyboard. The integration can be tested without the event,and left in the timed event table, by clearing the check box next to the Valley toValley event and then re-integrating the chromatogram.

7 Close the Integration Events table.
Practice adding and deleting integration timed events using the Multi
Calibration.dat data.

IPN 074-538-P1A

This completes the Tutorial chapter. Refer to earlier chapters of this manual or
online Help within the software for more detailed descriptions of each function.

13 - 26