Mari Iwase
Email: imari@u-shizuoka-ken.ac.jp

Education:Ph.D. (2016－ 2020)
Graduate School of Agriculture, Kyoto University, Japan
Dissertation: Studies on the endogenous and exogenous uncoupling protein 1 expression regulatory substances- M.S. (2014－ 2016) / B.S. (2010－ 2014)
Faculty of Agriculture, Kindai University, Japan.
Dissertation: Effect of Kaki (Japanese persimmon) Polyphenols on Glucose Absorption-Employment:- Washington University in St. Louis School of Medicine, Postdoc, 2020－ 2023
- Kyoto University, Graduate School of Agriculture, Postdoc, 2020

Technical Skill:
Recombinant DNA experiment- Cloning of interest genes- Plasmid construction- Expression of recombinant fusion-protein using E.coli
Cell-based analyses- Real-time PCR- Immunoblotting- Enzyme-linked immunosorbent assay (ELISA)- Transfection and cloning of stably transfected cell line
- Oxygen consumption measurement (Seahorse)
Cell culture- Adipocyte cell line (iWAT primary cells, 3T3-L1 cells)- Brown adipocyte cell line (HB2 cells)- Mesenchymal stem cell line (C3H10T1/2 cells)

Animal experiments- Breeding transgenic and knockout mice (includinggenotyping and primer design)- Dissection and blood sampling from tail vein
- Histological section (paraffin section: IHC and H&E)
- Organ analysis (DNA, RNA and protein expression)- Intraperitoneal injection/ Per os- Glucose/ insulin tolerance test
- Rectal temperature measurement- Blood chemical parameter measurement

Academic Award: Young Investigator Award, The 7th International Conference on Food Factors, Kobe, Japan, 2019.
 Best Presentation Award, The 72nd Japan Society of Nutrition and Food Science, Okayama, Japan, 2018
 TGBM poster award, The 2nd Society for Triglyceride Biology and Medicine, Nara, Japan, 2018.
 Excellent academic award, Kindai University, Japan, 2014

Publications:Journals
 C. Zhu, M. Iwase, Z. Li, F. Wang, A. Quinet, A. Vindigni, J. Shao, Profilin-1 regulates DNA replication forks in a contextdependent fashion by interacting with SNF2H and BOD1L, Nat Commun. 13 (2022) 6531.
 H. Takahashi, M. Tokura, S. Kawarasaki, H. Nagai, M. Iwase, K. Nishitani, H. Okaze, S. Mohri, T. Ito, T. Ara, H.F. Jheng, W.
Nomura, T. Kawada, K. Inoue, T. Goto, Metabolomics reveals inosine 5'-monophosphate is increased during mice adipocyte
browning, J Biol Chem. 298 (2022) 102456.
 K. Takemori, K. Akaho, M. Iwase, M. Okano, T. Kometani, Effects of Persimmon Fruit Polyphenols on Postprandial Plasma
Glucose Elevation in Rats and Humans, J Nutr Sci Vitaminol (Tokyo). 68 (2022) 331-341.
 S. Kawarasaki, H. Sawazaki, H. Iijima, S.P, Ng, J. Kwon, S. Mohri, M. Iwase, H.F. Jheng, H. Takahashi, W. Nomura, K.
Inoue, T. Kawada, T. Goto, Comparative Analysis of the Preventive Effects of Canagliflozin, a Sodium-Glucose CoTransporter-2 Inhibitor, on Body Weight Gain Between Oral Gavage and Dietary Administration by Focusing on Fatty AcidMetabolism, Diabetes Metab Syndr Obes. 13 (2020) 4353-4359.
 M. Iwase, S. Tokiwa, S. Seno, T. Mukai, Y.S. Yeh, H. Takahashi, W. Nomura, H.F. Jheng, S. Matsumura, T. Kusudo, N.
Osato, H. Matsuda, K. Inoue, T. Kawada, T. Goto, Glycerol kinase stimulates uncoupling protein 1 expression by regulating
fatty acid metabolism in beige adipocytes, J Biol Chem. 295(2020) 7033-7045.
 M. Iwase, S. Sakai, S. Seno, Y.S. Yeh, T. Kuo, H. Takahashi, W. Nomura, H.F. Jheng, P. Horton, N. Osato, H. Matsuda, K.
Inoue, T. Kawada, T. Goto, Long non-coding RNA 2310069B03Rik functions as a suppressor of Ucp1 expression under
prolonged cold exposure in murine beige adipocytes, Biosci Biotechnol Biochem. 84 (2020) 305-313.
 H. Hara, H. Takahashi, S. Mohri, H. Murakami, S. Kawarasaki, M. Iwase, N. Takahashi, M. Sugiura, T. Goto, T. Kawada, βCryptoxanthin Induces UCP-1 Expression via a RAR Pathway in Adipose Tissue, J Agric Food Chem. 67 (2019) 1059510603.
 Y.S. Yeh, H.F. Jheng, M. Iwase, M. Kim, S. Mohri, J. Kwon, S. Kawarasaki, Y. Li, H. Takahashi, T. Ara, W. Nomura, T.
Kawada, T. Goto, The Mevalonate Pathway Is Indispensable for Adipocyte Survival, iScience 9 (2018) 175-191.
 J.Y. An, H.F. Jheng, H. Nagai, K. Sanada, H. Takahashi, M. Iwase, N. Watanabe, Y.I. Kim, A. Teraminami, N. Takahashi, R.
Nakata, H. Inoue, S. Seno, H. Matsuda, T. Kawada, T. Goto, A phytol-enriched diet activates PPARα in the liver and brown
adipose tissue to ameliorate obesity-induced metabolic abnormalities, Mol Nutr Food Res. 62 (2018) e1700688.
 R. Ohue-Kitano, Y. Yasuoka, T. Goto, N. Kitamura, S.B. Park, S. Kishino, I. Kimura, M. Kasubuchi, H. Takahashi, Y. Li, Y.S.
Yeh, H.F. Jheng, M. Iwase, M. Tanaka, S. Masuda, T. Inoue, H. Yamakage, T. Kusakabe, F. Tani, A. Shimatsu, N. Takahashi,J. Ogawa, N. Satoh-Asahara, T. Kawada, alpha-Linolenic acid-derived metabolites from gut lactic acid bacteria inducedifferentiation of anti-inflammatory M2 macrophages through G protein-coupled receptor 40, FASEB J 32 (2018) 304-318.
 M. Iwase, T. Yamamoto, K. Nishimura, H. Takahashi, S. Mohri, Y. Li, H.F. Jheng, W. Nomura, N. Takahashi, C.S. Kim, R. Yu,

M. Taniguchi, K. Baba, S. Murakami, T. Kawada, T. Goto, Suksdorfin Promotes Adipocyte Differentiation and Improves
Abnormalities in Glucose Metabolism via PPARγ Activation, Lipids 52 (2017) 657-664.
 T. Goto, M. Hirata, Y. Aoki, M. Iwase, H. Takahashi, M. Kim, Y. Li, H.F. Jheng, W. Nomura, N. Takahashi, C.S. Kim, R. Yu,S. Seno, H. Matsuda, M. Aizawa-Abe, K. Ebihara, N. Itoh, T. Kawada, The hepatokine FGF21 is crucial for peroxisome
proliferator-activated receptor-α agonist-induced amelioration of metabolic disorders in obese mice, J Biol Chem. 292 (2017)9175-9190.

Conferences:Presentation- M. Iwase, C. Zhu, J. Shao, Stabilization of stressed DNA replication forks by VCP, The 3rd annual Siteman Cancer Research
Symposium, December 9, 2022, St. Louis, U.S.A- M. Iwase, C. Zhu, J. Shao, Stabilization of stressed DNA replication forks by phospho-Ser784-VCP, The Inaugural Retreat of
the Center for Genome Integrity, April 21-22, 2022, St. Louis, U.S.A- M. Iwase, S. Tokiwa, S. Seno, T. Mukai, H. Takahashi, W. Nomura, H.F. Jheng, T. Kusudo, N. Osato, H. Matsuda, K. Inoue, T.
Kawada, T. Goto, “Study on the relationship between fatty acid metabolism and thermogenic function of white adipocytes,”
The 7th International Conference on Food Factor, Dec. 1-5, 2019, Kobe, Japan- M. Iwase, S. Tokiwa, S. Seno, T. Mukai, H. Takahashi, W. Nomura, H.F. Jheng, T. Kusudo, N. Osato, H. Matsuda, K. Inoue,
T. Kawada, T. Goto, “Regulation mechanism of Ucp1 expression of glycerol kinase in adipose tissue,” The 40th Annual Meetingof Japan Society for the Study of Obesity, Nov. 2-3, 2019, Tokyo, Japan- M. Iwase, S. Tokiwa, S. Seno, T. Mukai, H. Takahashi, W. Nomura, H.F. Jheng, T. Ara, T. Kusudo, N. Osato, H. Matsuda, T.
Kawada, T. Goto, “Role of glycerol kinase in the browning of white adipose tissue,” The 73rd Japan Society of Nutrition andFood Science, May 18-19, 2019, Shizuoka, Japan- M. Iwase, S. Tokiwa, H. Takahashi, W. Nomura, H.F. Jheng, T. Ara, N. Osato, S. Seno, H. Matsuda, T. Kawada, T. Goto, “Roleof glycerol kinase in brown adipocyte function,” The 1st Japanese Society for Food Science and Technology Kansai branchmeeting, Nov. 22, 2018, Kyoto, Japan- M. Iwase, M. Hirata, H. Takahashi, H.F. Jheng, W. Nomura, T. Ara, N. Takahashi, S. Seno, H. Matsuda, M. Abe, K. Ebihara,
N. Itoh, T. Kawada, T. Goto, “Amelioration effect of metabolic abnormality through FGF21 at intake of PPARα agonist,” The
2nd Society for Triglyceride Biology and Medicine, Nov. 17, 2018, Nara, Japan- M. Iwase, M. Hirata, H. Takahashi, H.F. Jheng, W. Nomura, N. Takahashi, T. Kawada, T. Goto, “Peroxisome proliferatoractivated receptor-α agonist treatment ameliorates obesity and obesity-related glucose metabolism disorders in obese mice via
FGF21,” 25th European Congress on Obesity May 23-26, 2018, Vienna, Austria- M. Iwase, M. Hirata, H. Takahashi, H.F. Jheng, W. Nomura, T. Ara, N. Takahashi, S. Seno, H. Matsuda, M. Abe, K. Ebihara,
N. Itoh, T. Kawada, T. Goto, “FGF21 is important for peroxisome proliferator activated receptor alpha agonist-inducedamelioration of metabolic disorders in obese mice,” The 72nd Japan Society of Nutrition and Food Science, May 11-13, 2018,Okayama, Japan- M. Iwase, S. Sakai, S. Seno, T. Kuo, H. Takahashi, W. Nomura, H.F. Jheng, T. Ara, P. Horton, N. Osato, H. Matsuda, T. Kawada,T. Goto, “Search and function analysis of non-coding RNA controlling Ucp1 expression” The 2018 Annual Conference of theJapan Society for Bioscience, Biotechnology and Agrochemistry, Mar. 15-18, 2018, Nagoya, Japan- M. Iwase, K. Nishimura, H. Takahashi, N. Takahashi, M. Taniguchi, K. Baba, S. Murakami, T. Kawada, T. Goto, “Effects ofsuksdorfin on adipocyte differentiation and glucose and lipid metabolism disorders,” The 38th Annual Meeting of Japan Society
for the Study of Obesity, Oct. 7-8, 2017, Osaka, Japan- M. Iwase, K. Nishimura, H. Takahashi, N. Takahashi, M. Taniguchi, K. Baba, S. Murakami, T. Goto, T. Kawada, “Suksdorfin
improves metabolic abnormalities,” The 71st Japan Society of Nutrition and Food Science, May 19-21, 2017, Okinawa, Japan- M. Iwase, K. Nishimura, H. Takahashi, N. Takahashi, M. Taniguchi, K. Baba, S. Murakami, T. Goto, T. Kawada, “Suksdorfin
promotes adipocyte differentiation and improves abnormalities in glucose metabolism,” The 2017 Annual Conference of theJapan Society for Bioscience, Biotechnology and Agrochemistry, Mar. 18-20, 2017, Kyoto, Japan