11

Preparative Columns
Overview of column selection and method

development for isolation/purification---------------108-109
YMC-Actus series -----------------------------------110-113
Ordering Information--------------------------------114-116
YMC-GPC series ------------------------------------117-118

11Preparative Columns

Preparative packed columns
Overview of column selection and method development for isolation/purification
An overview for selecting optimum conditions for isolation/purification and check points are shown below.

Separation mode of chromatography
The following points should be considered for the selection of an appropriate separation mode:(1) Separation efficiency: Resolution, Loadability, Run time(2) Cost performance: Costs (packing materials/mobile phases), Throughput(3) Others: Safety, Characteristics of the target substances, Specification of the preparative LC system

Column size and particle size
Selection guide for the preparative column
When scaling up, column size and particle size of the packing materials should be selected depending on the amount of material andthe required purity of the target molecules. If flow rate and sample loading amount are increased proportionally to the ratio of the column
cross sectional areas whole keeping both column length and particle size of the packing materials, both the same resolution and thesame column pressure can be maintained before and after scaling up. Examples of typical scaling up studies are indicated by the arrowin the table below.

Lab scale

Production scale

Column inner diameter (mm)

4.6

10

20

30

50

100

200

500

1000

Cross sectional area ratio

1.0

4.7

19

42

118

473

1,890

11,80047,300

0.5

2.4

9.5

21

60

235

950

6,000
(6 L)

24,000(24 L)

1.0

4.7

19

42

120

470

1,900

12,000(12 L)

47,000(47 L)

Loading
(mg)

5

25

100

220

600

2,500

10,00060,000(60 g)

240,000(240 g)

5

●

●

●

●

◎

○

○

10

◎

●

●

●

●

◎

◎

◎

◎

10-20

○

◎

◎

◎

●

●

●

◎

◎

○

○

○

◎

●

●

●

◎

○

◎

◎

●

●

Flow rate(mL/min)

Example ofcalculation

Preparative Columns

High

Particle size(µm)

Columneﬃciency
Pressure
Costs

15-30
Low

50 -

● : Most appropriate

Flow rate equation *Use the same equation to calculate the sample load

F’=F × (Dc’/Dc)2

108

F : Analytical column ﬂow rate [mL/min] Dc : Analytical column inner diameter [mm]F’: Preparative column ﬂow rate [mL/min] Dc’: Preparative column inner diameter [mm]◎ : Appropriate ○ : Depending on purpose

Relationship of particle size and column efficiency/column backpressure
Smaller particle sizes lead to higher column efficiencies, but at a higher cost. In addition the systems with higher pressure limit are
required due to high backpressure. Smaller particle sizes are effective for higher resolution when the separation of the target peak
and the close peak is insufficient. On the other hand, larger particle sizes lead to lower column efficiencies, but at a lower the costs ofpacking materials and at a lower the column operating pressure.
Flow rate and column efficiency

0.08
0.06
0.04
0.02
0.00

0

0.5

1.0

Flow rate (mL/min)

1.5

Flow rate and column backpressure

10

Pressure (MPa)

HETP (mm/plate)

0.10

5 μm
10 μm
20 μm

5

0

0

0.5

1.0

Flow rate (mL/min)

1.5

Column: YMC-Triart C18
YMC-Triart Prep C18-S
150 X 4.6 mmI.D.
Eluent: acetonitrile/water (60/40)
Flow rate: 1.0 mL/min
Temperature : 25°C
Detection: UV at 254 nm
Sample: n-butyl benzoate

Method development
Preparative separation conditions are optimized with analytical scale column packed with the selected particle size.

(1) Separation conditions
When the particle size used in the preparative separation is larger than that used in the analytical scale, the required separation may
not be achieved due to lower column efficiencies. The separation may be improved by adjusting the mobile phase composition or
reducing the linear velocity. If the separation still doesn’t improve, the column length or the particle size needs to be changed.
(2) Loading study
Loading amount is gradually increased, and the obtained fractions are analyzed about purity and recovery rate. The appropriateloading amount is finally chosen based on the target purity and recovery.
(3) Preparative LC system evaluation
It is essential to make sure that the required flow rate and pressure are suitable for the specifications of the preparative LC system.
The same linear velocity used in analytical scale should be used to achieve the same resolution on the preparative column. Under
the same linear velocity, operating pressure on the preparative column is as almost same as that on the analytical column. If thecolumn pressure exceeds the limit of the system, the flow rate or column length needs to be reduced.
(4) Performing preparative separation

Preparative Columns

A preparative separation should be performed and the recovery and purity are evaluated.

109

11

Preparative Columns
High durability semi-preparative columns

YMC-Actus series
Axial Compression Technology for Ultimate Separation
Improved durability by applying axial compression technology
Prepacked column for milligram scale preparative HPLC
Excellent resolution
YMC-Actus series are semi-preparative HPLC columns that have excellent column durability and efficiency by applying axial
compression technology. YMC-Actus series columns show high durability under high flow rate or steep gradient conditions anddesirable for milligram scale preparative HPLC of various compounds.

Specifications
Pore size(Å)

Particle
size
(μm)

C%

Triart C18

120

5

20

Triart C18 ExRS

80

5

25

Triart C8

120

5

17

Triart Phenyl

120

5

17

1-10

Triart PFP

120

5

15

1-8

Triart Bio C4

300

5

—

1-10

Pro C18

120

5

16

Hydrosphere C18

120

5

12

Pro C18 RS

80

5

22

1-10

Pro C8

120

5

10

2-7.5

ODS-A
ODS-AQ

120
120

5
5

17
14

2-7.5

Packing material

UsablepH range

1-12

YMC-Triart

Pro series

YMC-Pack

Preparative Columns
110

2-8

Characteristics
Superior peak shapeUsable over wide range of pH and temperature
Usable with 100% aqueous mobile phase
Excellent selectivity of isomers and structural analogs
Superior chemical durability
Compete with the versatility of C18
Usable over wide range of pH and temperatureIdeal for separations of isomers or structural analogs
Unique selectivity due to π-π interaction
Excellent resolution without adsorption and tailing
Alternative selectivity to C18/C8 due to unique polar interaction
Superior planar cognitive ability/steric selectivity
Ideal for separations of compounds or isomers
Ideal for separation of biomolecules such as proteins andoligonucleotides
Good peak shapes with mobile phase suitable for LC/MSHigh performance ODS packing material
Can be used with 100% aqueous mobile phase
Superior separation for hydrophilic compounds
High carbon ODS packing material, high durability
Processed with advanced endcapping technology
Superior separation of basic compounds
Standard ODS from analytical to preparative
Good separation for hydrophilic compounds

Great durability achieved by applying axial compression technology[Excellent durability provided by improved bed density]

Attaching theend assembly

Packing
YMC-Actus

After continuous use under afast flow rate at high pressure
Flow

Compression

10% increase in
bed density

No changeWithcompression

after 700 run
initialFlow

Conventional Semi-preparative column

Without
compression

Void/crack in packed bed

Degradation of
performanceafter 300 run
initialColumn durability study
YMC-Actus

Test protocols

100

Conventional Preparative Column

80
60

Sequential gradient test

x 100 run

40

Column performance test

20
0

0

200

400

600

Preparative Columns% of initial theoretical plate number

120

800

run
Sequential gradient test
(high-speed and high-pressure)
Column size : 5 μm, 50 X 20 mmI.D. or 50 X 19 mmI.D.
Eluent: A) water B) methanol
Gradient: 5%B (0-0.5 min),
5-95%B (0.5-3.1 min),
95%B (3.1-3.6 min),
5%B (3.6-4.0 min)Flow rate: 50 mL/min
Pressure: Max.17 MPa
Column performance test
Column size : 5 μm, 50 X 20 mmI.D. or 50 X 19 mmI.D.
Eluent: methanol/water (60/40)
Flow rate: 10 mL/min
Sample: naphthalene

Uniformly high density packing is necessary for high performance HPLC column. DAC(Dynamic Axial Compression) column is widely used for preparative separation in pilot or
production scale. It allows uniformly high density packing and prevents formation of voids
during use by applying continuous compression.
YMC-Actus series have been developed by applying this Axial Compression Technology
to semi-prep column. This column bed is compressed adequately by attaching the end
assembly newly designed for YMC-Actus. It provides proper bed density (10% higher thanconventional columns) and results in higher efficiency and durability.

111

11

Preparative Columns
Separation at high loading[Purification of basic pharmaceutical: Clindamycin]

Purification method development
YMC-Triart C18 5 µm, 150 X 4.6 mmI.D.
0.01 mg Loading (10 µL injection, 1.0 mg/mL in water)

0.5 mg Loading (10 µL injection, 50 mg/mL in water)

Increasing
loading amount

0.40

Clindamycin

2.50
2.00
AU

0.30
AU

pH 2.7

3.00

0.20
0.10
0.00

10.0

15.0

130228101136

5.0

min

10.0

2.00
AU

0.20

1.50
1.00
0.50
0.00

130227135854

5.0

10.0

15.0

130227144916

min

5.0

10.0

Impact of loading amounton peak shape: Low
0.40

Effective for purification
at high loading

2.50
2.00

0.20

AU

AU

15.0 min

3.00

0.30

0.10
0.00

min

2.50

0.10

pH 9.815.0

Impact of loading amounton peak shape: Medium

3.00

0.30
AU

1.00
0.00

130222203522

0.40

0.00

1.50
0.50

5.0

pH 6.7

Impact of loading amounton peak shape: High

1.50
1.00
0.50
0.00

130228181208

5.0

10.0

15.0

min

130306124728

5.0

10.0

15.0 min

CH3
Cl

N
H
N

H3C

Purification at pH 9.8

O

YMC-Actus Triart C18 5 µm, 150 X 20 mmI.D.

CH3

OH
OH

S

Clindamycin pKa=7.6

Preparative Columns(x 100)50 mg Loading

6.0
4.0

Fraction

2.0
0.0

13030633602

0.0

2.5

5.0

7.5

OH

CH3

Eluent(250 µL injection, 200 mg/mL in water)

8.0

O

10.0

12.5

15.0

min

: A) 20 mM HCOOH for pH 2.7
20 mM HCOONH4 for pH 6.7
20 mM HCOONH4-NH3 for pH 9.8
B ) acetonitrile
10-75%B (0-15 min)Flow rate: 1.0 mL/min for 150 X 4.6 mmI.D.
18.9 mL/min for 150 X 20 mmI.D.
Temperature : 25°C for 150 X 4.6 mmI.D.
ambient for 150 X 20 mmI.D.
Detection: UV at 210 nm
Pressure: 7.0 MPa for 150 X 4.6 mmI.D.
8.4 MPa for 150 X 20 mmI.D.

Fraction analysis
Purity : ≧99.9%
Recovery : 86%

Clindamycin

0.40
AU

0.30
0.20
0.10

130301211148

0.00

0.0

4.0

8.0

12.0

min

Column: YMC-Triart C18 5 μm
150 X 4.6 mmI.D.
Eluent: 50 mM KH2PO4 (pH 7.5 adjusted by 8 M KOH)/
acetonitrile (55/45)
Flow rate: 1.0 mL/min
Temperature : 25°C
Detection: UV at 210 nm
Injection: 20 μL

Clindamycin and its impurities (related compounds) are more hydrophobic in their un-ionized form and are retained stronger at pH 9.8. At higher pH condition,
the resolution between main peak and impurities is improved and the peak shape is less affected by increase of mass loading.
Excellent chemical durability of YMC-Triart offers an option of purification at a high pH that is effective for basic compounds by increasing retention and massloading. Moreover, highly efficient YMC-Actus Triart has identical performance to YMC-Triart analytical column. This enables direct scale up from analyticalcondition to preparative condition. The combination of YMC-Triart and YMC-Actus offers highly efficient purification of various compounds.

112

Purification of hydrophobic compounds with similar structure –Capsaicinoids in red pepper–
Analysis
YMC-Pack Pro C18 RS 5 μm
50 X 4.6 mmI.D.
2.0 mL/min, 20 µL injection

Brand G2 5 µm
50 X 4.6 mmI.D.
2.0 mL/min, 20 µL injection
2

2

2
3

3
1

0

2.5

Brand I8 5 µm
50 X 4.6 mmI.D.
2.0 mL/min, 20 µL injection

3
1

1

5.0

7.5

10.0 min

0

2.5

5.0

7.5

10.0 min

0

2.5

5.0

7.5

10.0 minPurification
YMC-Actus Pro C18 RS 5 μm
50 X 20 mmI.D.
40 mL/min, 400 µL injection

1.

After
purification 5 µm, 50 X 4.6 mmI.D.

O

H3CO

Capsaicin

HO

Purity > 99.9%

CH3

N
H

CH3

Nordihydrocapsaicin

2
2.

O

H3CO
HO

3
0

5.0

3.

O

H3CO
HO
2.5

5.0

7.5

CH3

Capsaicin

10.0 min

1

0.0

CH3

N
H

N
H

CH3
CH3

Dihydrocapsaicin

10.0 min

Pro C18 RS has superior selectivity for hydrophobic compounds that differ
slightly in structure and hydrophobicity, achieves better resolution between
peak 1 and peak 2.
Furthermore, analytical separation can be directly scaled up to preparative
scale with YMC-Actus Pro C18 RS. YMC-Actus series have high resolution
equal to analytical columns.

Eluent: A) methanol/water/TFA (50/50/0.1)
B) methanol/TFA (100/0.1)
10-30%B (0-5 min), 30%B (5-10 min)Temperature : 25°C for 50 X 4.6 mmI.D.
ambient for 50 X 20 mmI.D.
Detection: UV at 280 nm
Sample: methanol extract of a commercial cayenne pepper(1 g cayenne pepper/3 mL)

Crude synthetic 30 mer oligonucleotide

Analysis
Hydrosphere C18 5 µm
50 X 4.6 mmI.D.
1.0 mL/min, 5 µL injection

5'-CCGCTCGAGCTAAAAAAAGCCTGTGTTACC-3'

Brand I1 5 µm
50 X 4.6 mmI.D.
1.0 mL/min, 5 µL injection

Eluent: A) 10 mM DBAA* (pH 6.0)/methanol (60/40)
B) 10 mM DBAA* (pH 6.0)/methanol (20/80)
10-35%B (0-30 min)Temperature : ambient
Detection: UV at 269 nm
Sample: synthetic oligonucleotide (100 μM)

30 mer
Impurities

30 mer

Impurities

Preparative Columns

Purification of highly polar compounds –Oligonucleotide–* di-n-butylammonium acetate
0

10

20

30 min0

10

20

30 minPurification
YMC-Actus Hydrosphere C18 5 µm
50 X 20 mmI.D.
19 mL/min, 100 µL injection

Brand I1 5 µm
50 X 19 mmI.D.
19 mL/min, 100 µL injection

purity>99%

purity>99%

Recovery 56%

20

22.5

25

27.5 minIn analytical scale, many impurities could be separated
from the target compound by onenucleotide difference on
Hydrosphere C18. Even in purification scale, YMC-Actus
gave superior separation and recovery.
YMC-Actus Hydrosphere C18 is useful for purification ofhydrophilic compounds such as oligonucleotides, organic
acids, oligosaccharides and glycosides.

Recovery 35%

7.5

10

12.5 min113

11

Preparative Columns
Ordering Information –Columns–
YMC-Actus series (Pressure limit : 30 MPa for 20 and 30 mmI.D., 20 MPa for 50 mmI.D.)
Particlesize(μm)

Column size
inner diameter X
length (mm)Triart C18(120 Å)5

20 X 50
20 X 100
20 X 150
20 X 250
30 X 50
30 X 75
30 X 100
30 X 150
30 X 250
50 X 250

TA12S05-0520WXTA12S05-1020WX
TA12S05-1520WXTA12S05-2520WXTA12S05-0530WXTA12S05-L530WXTA12S05-1030WX
TA12S05-1530WXTA12S05-2530WXTA12S05-2553AX

Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 50
20 X 100
30 X 50
30 X 75
30 X 100

Triart C18 ExRS
(80 Å)

Triart C8(120 Å)Triart Phenyl(120 Å)Triart PFP(120 Å)TAR08S05-0520WX TO12S05-0520WX TPH12S05-0520WX TPF12S05-0520WXTAR08S05-1020WX TO12S05-1020WX TPH12S05-1020WX TPF12S05-1020WX
TAR08S05-1520WX TO12S05-1520WX TPH12S05-1520WX TPF12S05-1520WXTAR08S05-2520WX TO12S05-2520WX TPH12S05-2520WX TPF12S05-2520WXTAR08S05-0530WX TO12S05-0530WX TPH12S05-0530WX TPF12S05-0530WXTAR08S05-L530WX TO12S05-L530WX TPH12S05-L530WX TPF12S05-L530WXTAR08S05-1030WX TO12S05-1030WX TPH12S05-1030WX TPF12S05-1030WX
TAR08S05-1530WX TO12S05-1530WX TPH12S05-1530WX TPF12S05-1530WXTAR08S05-2530WX TO12S05-2530WX TPH12S05-2530WX TPF12S05-2530WX————TB30S05-0520WXTB30S05-1020WX
TB30S05-1520WXTB30S05-2520WXTB30S05-0530WXTB30S05-L530WXTB30S05-1030WX
TB30S05-1530WXTB30S05-2530WX—YMC-Pack
Pro C18 RS(80 Å)YMC-Pack Pro C8(120 Å)YMC-Pack ODS-A YMC-Pack ODS-AQ(120 Å)(120 Å)HS12S05-0520WXHS12S05-1020WX
HS12S05-0530WX
HS12S05-L530WX
HS12S05-1030WX

RS08S05-0520WX
RS08S05-1020WX
RS08S05-0530WX
RS08S05-L530WX
RS08S05-1030WX

OS12S05-0520WXOS12S05-1020WX
OS12S05-0530WX
OS12S05-L530WX
OS12S05-1030WX

AA12S05-0520WXAA12S05-1020WX
AA12S05-0530WXAA12S05-L530WXAA12S05-1030WX

AQ12S05-0520WXAQ12S05-1020WX
AQ12S05-0530WX
AQ12S05-L530WX
AQ12S05-1030WX

Triart C18 ExRS
(80 Å)

Triart C8(120 Å)Triart Phenyl(120 Å)Triart PFP(120 Å)Triart Bio C4(300 Å)YMC-Pack Pro C18 Hydrosphere C18(120 Å)(120 Å)AS12S05-0520WXAS12S05-1020WX
AS12S05-0530WXAS12S05-L530WXAS12S05-1030WX

Triart Bio C4(300 Å)YMC-Actus series guard cartridges
Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 1030 X 10

Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 1030 X 10

Triart C18(120 Å)TA12S05-0120CCN TAR08S05-0120CCN TO12S05-0120CCN TPH12S05-0120CCN TPF12S05-0120CCN TB30S05-0120CCN
TA12S05-0130CCN TAR08S05-0130CCN TO12S05-0130CCN TPH12S05-0130CCN TPF12S05-0130CCN TB30S05-0130CCN

YMC-Pack Pro C18 Hydrosphere C18(120 Å)(120 Å)YMC-Pack
Pro C18 RS(80 Å)YMC-Pack Pro C8(120 Å)YMC-Pack ODS-A YMC-Pack ODS-AQ(120 Å)(120 Å)AS12S05-0120CCN HS12S05-0120CCN RS08S05-0120CCN OS12S05-0120CCN AA12S05-0120CCN AQ12S05-0120CCN
AS12S05-0130CCN HS12S05-0130CCN RS08S05-0130CCN OS12S05-0130CCN AA12S05-0130CCN AQ12S05-0130CCN*Guard cartridge holder required, part no. XPGHFSP20ID for 20 mmI.D. and XPGHFSP30ID for 30 mmI.D.

Preparative Columns
114Ordering Information –Columns–
Preparative columns (Pressure limit : 10 MPa)
Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 150
20 X 250
30 X 150

YMC-Pack Pro C18 Hydrosphere C18(120 Å)(120 Å)AS12S05-1520WTAS12S05-2520WTAS12S05-1530WT

Particle
Column size
size inner diameter X(μm)
length (mm)HS12S05-1520WT
HS12S05-2520WT—YMC-Pack
Pro C18 RS(80 Å)YMC-Pack Pro C8(120 Å)YMC-Pack Pro C4(120 Å)RS08S05-1520WT
RS08S05-2520WT——OS12S05-2520WT——BS12S05-2520WT—YMC-Pack ODS-A(120 Å)(200 Å)YMC-Pack ODS-AQ(300 Å)Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 100
20 X 150
20 X 250
30 X 75
30 X 100
30 X 150
30 X 250
50 X 250

Particlesize(μm)

Column size
inner diameter X
length (mm)

YMC-Pack TMS(120 Å)YMC-Pack Ph(120 Å)5

20 X 100
20 X 150
20 X 250
30 X 75
30 X 100
30 X 150
30 X 250
50 X 250

TM12S05-1020WT
TM12S05-1520WTTM12S05-2520WTTM12S05-L530WT
TM12S05-1030WT
TM12S05-1530WT
TM12S05-2530WT
TM12S05-2552AR

PH12S05-1020WT
PH12S05-1520WT
PH12S05-2520WT
PH12S05-L530WT
PH12S05-1030WT
PH12S05-1530WT
PH12S05-2530WT
PH12S05-2552AR

Particlesize(μm)

Column size
inner diameter X
length (mm)

YMC-Pack SIL(120 Å)YMC-Pack SIL-06(60 Å)

5

20 X 100
20 X 150
20 X 250
30 X 75
30 X 100
30 X 150
30 X 250
50 X 250

SL12S05-1020WT
SL12S05-1520WTSL12S05-2520WTSL12S05-L530WTSL12S05-1030WT
SL12S05-1530WTSL12S05-2530WTSL12S05-2552AR

SL06S05-1020WT
SL06S05-1520WT
SL06S05-2520WT
SL06S05-L530WT
SL06S05-1030WT
SL06S05-1530WT
SL06S05-2530WT
SL06S05-2552AR

Particlesize(μm)

Column size
inner diameter X
length (mm)420 X 150
20 X 250(200 Å)YMC-Pack ODS-AM YMC-Pack ODS-AL(120 Å)(120 Å)—————AM12S05-1020WT AL12S05-1020WT
AA12S05-1520WT AA20S05-1520WT AA30S05-1520WT AQ12S05-1520WT AQ20S05-1520WT AM12S05-1520WT AL12S05-1520WTAA12S05-2520WT AA20S05-2520WT AA30S05-2520WT AQ12S05-2520WT AQ20S05-2520WT AM12S05-2520WT AL12S05-2520WT
—
—
—
—
—
AM12S05-L530WT AL12S05-L530WT
—
—
—
—
—
AM12S05-1030WT AL12S05-1030WT
AA12S05-1530WT——
AQ12S05-1530WT—
AM12S05-1530WT AL12S05-1530WT
AA12S05-2530WT AA20S05-2530WT AA30S05-2530WT AQ12S05-2530WT AQ20S05-2530WT AM12S05-2530WT AL12S05-2530WT
AA12S05-2552AR AA20S05-2552AR AA30S05-2552AR AQ12S05-2552AR AQ20S05-2552AR AM12S05-2552AR AL12S05-2552AR

YMC-Pack C4

YMC-Pack C8(120 Å)(200 Å)(300 Å)(120 Å)(200 Å)(300 Å)—BU20S05-1520WTBU20S05-2520WT———BU20S05-2530WTBU20S05-2552AR—
BU30S05-1520WTBU30S05-2520WT———BU30S05-2530WTBU30S05-2552AR

YMC-Pack
PROTEIN-RP

YMC Carotenoid

CN12S05-1020WT—
CN12S05-1520WT CN30S05-1520WT
CN12S05-2520WT CN30S05-2520WT
CN12S05-L530WT—
CN12S05-1030WT—
CN12S05-1530WT—
CN12S05-2530WT CN30S05-2530WT
CN12S05-2552AR CN30S05-2552AR—
PR99S05-1520WTPR99S05-2520WT
—
—
—
—
—

—
CT99S05-1520WTCT99S05-2520WT
—
—
—
—
—

YMC-Pack Diol-NP

YMC-Pack
Polyamine Ⅱ(120 Å)YMC-Pack NH2(120 Å)——PB12S05-2520WT
—
—
—
—
—

—
NH12S05-1520WTNH12S05-2520WT———NH12S05-2530WT—OC12S05-1020WT——
BU12S05-1020WT
OC12S05-1520WT OC20S05-1520WT OC30S05-1520WT BU12S05-1520WTOC12S05-2520WT OC20S05-2520WT OC30S05-2520WT BU12S05-2520WTOC12S05-L530WT——BU12S05-L530WTOC12S05-1030WT——
BU12S05-1030WT
OC12S05-1530WT——BU12S05-1530WTOC12S05-2530WT OC20S05-2530WT OC30S05-2530WT BU12S05-2530WTOC12S05-2552AR OC20S05-2552AR OC30S05-2552AR BU12S05-2552AR

YMC-Pack CN(120 Å)(60 Å)(300 Å)(120 Å)——DN06S05-1520WT DN12S05-1520WT
DN06S05-2520WT DN12S05-2520WT
—
—
—
—
—
—
—
—
—
—

Preparative Columns

5

20 X 100
20 X 150
20 X 250
30 X 75
30 X 100
30 X 150
30 X 250
50 X 250(120 Å)J'sphere ODS-H80 J'sphere ODS-M80 J'sphere ODS-L80(80 Å)(80 Å)(80 Å)
JH08S04-1520WT
JH08S04-2520WTJM08S04-1520WTJM08S04-2520WTJL08S04-1520WTJL08S04-2520WT

115

11

Preparative Columns
Ordering Information –Columns–
Guard columns
Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 50
30 X 50

YMC-Pack Pro C18 Hydrosphere C18(120 Å)(120 Å)YMC-Pack
Pro C18 RS(80 Å)YMC-Pack Pro C8(120 Å)YMC-Pack Pro C4(120 Å)AS12S05-0520WTG HS12S05-0520WTG RS08S05-0520WTG OS12S05-0520WTG BS12S05-0520WTG
AS12S05-0530WTG————

Particle
Column size
size
inner diameter X(μm)
length (mm)

YMC-Pack ODS-A(120 Å)(200 Å)YMC-Pack ODS-AQ(300 Å)(120 Å)Preparative Columns

5

20 X 50
30 X 50
50 X 50

Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 50
30 X 50
50 X 50

Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 50
30 X 50
50 X 50

Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 50
30 X 50
50 X 50

Particlesize(μm)

Column size
inner diameter X
length (mm)J'sphere ODS-H80 J'sphere ODS-M80 J'sphere ODS-L80(80 Å)(80 Å)(80 Å)

4

20 X 50

JH08S04-0520WTG JM08S04-0520WTG JL08S04-0520WTG(200 Å)YMC-Pack ODS-AM YMC-Pack ODS-AL(120 Å)(120 Å)AA12S05-0520WTG AA20S05-0520WTG AA30S05-0520WTG AQ12S05-0520WTG AQ20S05-0520WTG AM12S05-0520WTG AL12S05-0520WTG
AA12S05-0530WTG AA20S05-0530WTG AA30S05-0530WTG AQ12S05-0530WTG AQ20S05-0530WTG AM12S05-0530WTG AL12S05-0530WTG
AA12S05-0552ARG AA20S05-0552ARG AA30S05-0552ARG AQ12S05-0552ARG AQ20S05-0552ARG AM12S05-0552ARG AL12S05-0552ARG

YMC-Pack C4

YMC-Pack C8(120 Å)(200 Å)(300 Å)(120 Å)(200 Å)(300 Å)OC12S05-0520WTG OC20S05-0520WTG OC30S05-0520WTG BU12S05-0520WTG BU20S05-0520WTG BU30S05-0520WTG
OC12S05-0530WTG OC20S05-0530WTG OC30S05-0530WTG BU12S05-0530WTG BU20S05-0530WTG BU30S05-0530WTG
OC12S05-0552ARG OC20S05-0552ARG OC30S05-0552ARG BU12S05-0552ARG BU20S05-0552ARG BU30S05-0552ARG

YMC-Pack TMS(120 Å)YMC-Pack Ph(120 Å)YMC-Pack CN(120 Å)(300 Å)YMC-Pack
PROTEIN-RP

TM12S05-0520WTG PH12S05-0520WTG CN12S05-0520WTG CN30S05-0520WTG PR99S05-0520WTG
TM12S05-0530WTG PH12S05-0530WTG CN12S05-0530WTG CN30S05-0530WTG—
TM12S05-0552ARG PH12S05-0552ARG CN12S05-0552ARG CN30S05-0552ARG—

YMC-Pack SIL(120 Å)YMC-Pack SIL-06(60 Å)YMC-Pack
Polyamine Ⅱ(120 Å)YMC-Pack NH2(120 Å)SL12S05-0520WTG SL06S05-0520WTG PB12S05-0520WTG NH12S05-0520WTG
SL12S05-0530WTG SL06S05-0530WTG—
NH12S05-0530WTG
SL12S05-0552ARG SL06S05-0552ARG——CHIRAL ART preparative columns (Pressure limit : 30 MPa)
Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 250
30 X 250

Immobilized type
Amylose-SA

Cellulose-SB

Cellulose-SC

Coated type
Cellulose-SJ

Amylose-C Neo

Cellulose-C

KSA99S05-2520WX KSB99S05-2520WX KSC99S05-2520WX KSJ99S05-2520WX KBN99S05-2520WX KCN99S05-2520WXKSA99S05-2530WX KSB99S05-2530WX KSC99S05-2530WX KSJ99S05-2530WX KBN99S05-2530WX KCN99S05-2530WX

Alcyon SFC CSP preparative columns (Pressure limit : 20 MPa)
Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 250

Immobilized type
Amylose-SA

Cellulose-SB

Cellulose-SC

Coated type
Cellulose-SJ

Amylose-C Neo

Cellulose-C

KSA99S05-2520WTS KSB99S05-2520WTS KSC99S05-2520WTS KSJ99S05-2520WTS KBN99S05-2520WTS KCN99S05-2520WTSCHIRAL ART guard cartridges
Particlesize(μm)

Column size
inner diameter X
length (mm)520 X 1030 X 10

Immobilized type
Amylose-SA

Cellulose-SB

Cellulose-SC

Coated type
Cellulose-SJ

Amylose-C Neo

Cellulose-C

KSA99S05-0120CCN KSB99S05-0120CCN KSC99S05-0120CCN KSJ99S05-0120CCN KBN99S05-0120CCN KCN99S05-0120CCN
KSA99S05-0130CCN KSB99S05-0130CCN KSC99S05-0130CCN KSJ99S05-0130CCN KBN99S05-0130CCN KCN99S05-0130CCN*Guard cartridge holder required, part no. XPGHFSP20ID for 20 mmI.D. and XPGHFSP30ID for 30 mmI.D.

116

YMC-GPC series
Suitable for separation of polymer or oligomer on the basis of molecular weightCompatible with organic solvents with various polarities
High resolution and long lifetime under a high flow rate condition
High productivity by fast separation
Ideal for recycling GPC that can improve resolution

Polymer based preparative GPC columns
YMC-GPC is a column packed with highly cross-linked porous polystyrene/divinylbenzene media. It provides outstanding physicalrigidity for extended lifetimes especially at a high temperatures and in aggressive solvents. YMC-GPC offers high productivity on
preparative separation due to high resolution and high loadability, at a fast flow rate. Furthermore, higher resolution can be achieved
on a sample that is hardly separated in combination with recycling chromatography method, even without changing mobile phaseconditions or columns.

Compatible with various organic solvents

Low polarity

Preparative Columns

High polarity

YMC-GPC has excellent solvent versatility. It can be transferred
easily and rapidly between solvents of varying polarity. It is possible
to select the optimum mobile phase depending on the solubility andseparation behavior of the sample.

Perfluoroalkane
HexaneCyclohexane
TolueneEthyl acetate
Tetrahydrofuran (THF)
Chloroform
Methyl ethyl ketone (MEK)
Dichloromethane
Dichloroethene
Acetoneo-Dichlorobenzene (o-DCB)
Trichlorobenzene (TCB)
m-Cresolo-Chlorophenol (o-CP)
Pyridine
Dimethyl acetamide (DMAc)
n-Methyl pyrrolidone (NMP)
Dimethyl sulfoxide (DMSO)
Dimethyl formamide (DMF)

YMC-GPC is normally supplied in ethylbenzene unless otherwise
stated. The transferring procedure to other mobile phases is
described in the instruction manual on our website.

Calibration curves

Polystyrene Molecular Weight

10E6

ColumnEluentFlow rate
Sample100 nm
50 nm
10 nm
5 nm

10E5

Calibration curves of polystyrene by YMC-GPC are shown left.
The calibration curves are designed to be linear over a specified
molecular weight range, ensuring that the same degree of resolutionis achieved across the full operating range of the column.
Desired separation can be achieved by selecting a column
depending on the molecular weight range of a sample.

10E4

10E3

10E2
4

: 300 X 7.5 mmI.D.
: THF: 1.0 mL/min: polystyrene

5

6

7

8

9

10

Elution volume (mL)

117

11

Preparative Columns
Recycling chromatography separation of polystyrene oligomers
ColumnmAU

EluentFlow rate
Detection
SampleInjection

500
1st
400

300

Recycling separation of polystyrene oligomers by YMC-GPC T4000
column is shown. By using recycling chromatography separation
method, higher resolution can be achieved on a sample that ishardly separated, even without changing mobile phase conditions
or columns. Furthermore, no solvent is consumed during recycling.
It greatly contributes to reduction of solvent consumption on
purification.

2nd
3rd

200

4th

: YMC-GPC T4000 (10 μm, 100 Å)
600 X 21.2 mmI.D.
: chloroform: 10 mL/min: UV at 254 nm: polystyrene oligomers (50 mg/mL): 1.4 mL

5th

100

0

G130904B

0

20

40

60

min

Ordering Information –Columns–
YMC-GPC (Theoretical plate number > 20,000)

Preparative Columns
118

Product namePhase
dimension

YMC-GPC T2000
YMC-GPC T2000-40
YMC-GPC T4000
YMC-GPC T4000-40
YMC-GPC T30000
YMC-GPC T30000-40
YMC-GPC T60000
YMC-GPC T60000-40
YMC-GPC T10M
YMC-GPC T10M-40

50 Å
10 μm
100 Å
10 μm
500 Å
10 μm
1000 Å
10 μm
MIX
10 μm

Molecular weight range(g/mol)- 2,000- 4,000
500 - 30,000
500 - 60,000
500 - 10,000,000

Column size
inner diameter X length (mm)Product number

21.2 X 600
40 X 600
21.2 X 600
40 X 600
21.2 X 600
40 X 600
21.2 X 600
40 X 600
21.2 X 600
40 X 600

GP05S10-6020PT
GP05S10-6040WT
GP10S10-6020PT
GP10S10-6040WT
GP50S10-6020PT
GP50S10-6040WTGPA0S10-6020PT
GPA0S10-6040WT
GP9BS10-6020PT
GP9BS10-6040WT