Sensitivity · Selectivity · Robustness

YMC Capillary Columns
for MicroLC and NanoLC

Drug Discovery
Life Science
Bioanalysis
Food
Environmental

Capillary Columns

Introduction
Miniaturisation of liquid chromatography in combination with mass spectrometry has several advantages
including improvements in sensitivity, especially at low
concentration levels and dramatically reduced solvent
consumption, compared to conventional HPLC or
UHPLC.
With further method optimisation, run time can also
be reduced and therefore more solvent or time can besaved. To meet the requirements of MicroLC/NanoLC
YMC offers capillary columns especially designed for
rapid high-resolution gradient chromatography.

Selectivity:• All fully porous YMC-phases• Phases for RP, NP, HILIC, chiral LC and SEC• Variety of dimensions• Particles from 1.9 to 5 µm

Sensitivity:• Extremely low sample volumes and flow rates• Compatible with most Micro-/NanoLC/MS systems• Guard columns, also for trapping/desalting

Robustness:• Long column lifetimes• Exceptional column-to-column reproducibility• Excellent lot-to-lot reproducibility

2

Contents
			

page

Capillary column efficiencies........................................................................................................................................................ 4–5

MicroLC applications................................................................................................................................................................................................ 6–17
Clinical.............................................................................................................................................................................................................................................6–7
Biopharma................................................................................................................................................................................................................................ 8–11
Food............................................................................................................................................................................................................................................. 12–17

Solutions for small molecules.................................................................................................................................................18–19

Columns for biomolecules............................................................................................................................................................. 20–21

YMC capillary column hardware....................................................................................................................................... 22–23

Ordering information................................................................................................................................................................................ 24–26

3Capillary Columns

Columns in micro format

YMC MicroLC columns: highest efficiency
YMC-Triart C18 MicroLC columns packed with 1.9 μm particles show the highest column efficiency in an efficiency
study using van Deemter analysis. Results for YMC MicroLC columns show:•

increased resolution•

increased separation speed•

reduced solvent consumption•

reduced cycle times

Highest Efficiency: Van Deemter analysis
The diagrams show the reduced plate heights (h) of a YMC-Triart C18 MicroLC column together with two otherMicroLC columns as a function of the linear velocity at different %B. A low h-value means high column efficiency.

AB
1.9 µm YMC Triart-C18
1.6 µm fully porous
1.8 µm core-shell

1.9 µm YMC Triart-C18
1.6 µm fully porous
1.8 µm core-shell

Van Deemter plots for naphthalene at retention factors of k’ = 2 [A] and k’ = 11 [B]. Experimental setup according to Hetzel et al. [1].

The YMC-Triart C18 MicroLC column shows the highest column efficiency for both retention factorsof naphthalene. Neither the column packed with the nominally smaller fully porous particles, nor theone packed with the Core-Shell media show efficiencies approaching that of the YMC-Triart C18
column. Therefore, YMC-Triart MicroLC columns show higher efficiency.
Van Deemter plots by courtesy of: Tobias Werres, IUTA - Institut für Energie- und Umwelttechnik e. V., Duisburg, Germany.

[1] T. Hetzel, et al., Characterization of the efficiency of microbore liquid chromatography columns by van
Deemterand kinetic plot analysis, J.Sep.Sci. (2016).

4

Capillar y column ef ficiencies

Excellent column-to-column reproducibility
2.5

Theoretical plate number
15000

2.0

10000

1.5

Tailing factor
5000

1.0

0

Column:Part No.:
Eluent:Tailing factor

Theoretical plate number

20000

10

20

30

40

Column number

YMC-Triart C18 (5 µm) 150 x 4.6 mm ID
TA12S05-1546PTH
acetonitrile / water (40/60)

Flow rate:
Temperature:Sample:0.550

1.0 mL/minambientbutyl benzoate

The reproducibility of packed columns is shown in terms of theoretical plate number (N) and tailingfactor (Tf) for a number of different packed columns. The same is true for YMC capillary columns,
which exhibit a very narrow range of variation, also due to the excellent lot-to-lot reproducibly of
the packing material.

Excellent batch-to-batch reproducibility
Basic compounds

1

Coordination compounds

23

1. Hinokitiol (coordination)
2. Methyl benzoate

1. Chlorpheniramine (basic)
2. Dextromethorphan (basic)
3. Propyl p-hydroxybenzoate

1

2

Lot. E

Lot. E

Lot. D

Lot. D

Lot. C

Lot. C

Lot. B

Lot. B

Lot. A0.0

2.5

J091201Y

Column:Part No.:
Eluent:Flow rate:
Temperature:
Detection:

5.0

7.5

10.0

12.5

15.0

YMC-Triart C18 (5 µm) 150 x 3.0 mm ID
TA12S05-1503PTH
20 mM KH2PO4 (pH 6.9) / acetonitrile (65/35)
0.425 mL/min
40 °C
UV at 235 nm

17.5

min

Lot. A
0.0

J091201X

2.0

Column:Part No.:
Eluent:Flow rate:
Temperature:
Detection:

4.0

6.0

8.0

10.0

12.0

14.0

min

YMC-Triart C18 (5 µm) 150 x 3.0 mm ID
TA12S05-1503PTH
acetonitrile / 0.1% H3PO4 (40/60)
0.425 mL/min
40 °C
UV at 254 nm

Excellent reproducibility of YMC-Triart phases is available even for the analysis of basic and coordination compounds which normally exhibit tailing and adsorption effects.

5Capillary Columns

MicroLC applications

High sensitivity analysis of antineoplastic
drugs from wipe samples
Standards of 11 antineoplastic drugs(1) Gemcitabine(2) Methotrexate(7) Doxorubicin(3) Topotecan(8) Epirubicin(4) Irinotecan(9) Etoposide(5) Ifosfamide(10) Paclitaxel(11) Docetaxel

4.5E+05100

4.0E+05180

3.5E+05
3.0E+057

2.5E+0522.0E+053

60%B

intensity / cps(6) Cyclophosphamide

4/6

40

1.5E+05

5 8

1.0E+0520

95.0E+0411
10

0.0E+00
0.0

0.5

1.0

1.5

2.0

0

time / min
YMC-Triart C18 (12 nm, 1.9 μm) 50 x 0.3 mm ID, 1/32" end fittings
TA12SP9-05H0RUA) H2O + 0.1% formic acidB) acetonitrile + 0.1% formic acid
Gradient:
10%B (0 min), 50%B (1.6 min), 99%B (2.5 min)Flow rate:
25 μL/min
Temperature: 40 °C
Column:Part No.:
Eluent:Detection:
Injection:
Sample:LC system:SCIEX QTRAP 6500, ESI
4.25 µL(1) gemcitabine, (2) methotrexate, (3) topotecan, (4) irinotecan,(5) ifosfamide, (6) cyclophosphamide, (7) doxorubicin,(8) epirubicin, (9) etoposide, (10) paclitaxel, (11) docetaxel (1 µg/mL)
Eksigent ExpressLC ultra system

Separation of the standard samples containing 11 antineoplastic drugs. As the German substanceindependent reference value for wipe samples is 0.1 ng/cm² the method was optimised to a LOQ of
0.01 ng/mL as described before by Hetzel et al [1].

6MicroLC applications

Wipe samples from different hospital locations

a

b

c

Chromatograms for 3 different real wipe samples taken from different locations in a hospital andapplication areas in hospital wards according to Hetzel et al. The applicability of the developed
method according to Hetzel et al. for real samples and the possibility to use this method in the daily
life of the pharmaceutical sector is shown [1].
Application data by courtesy of: Thorsten Teutenberg, IUTA - Institut für Energie- und Umwelttechnik e. V., Duisburg, Germany.

[1] T. Hetzel, et al., Micro-liquid chromatography mass spectrometry for the analysis of antineoplastic drugs from    wipe samples, Anal. Bioanal. Chem. (2016).

7

Capillary Columns

Increased sensitivity in peptide analysis
Analysis of 9 different Pierce™ peptides by UHPLC and MicroLC

4.43

100

2.1>1.0: 4.2x
1.0>0.5: 1.9x
Total gain: 8.0x
(area)

80
60
4.43
40
4.43

20

gain
Up to 13 x
ity
in sensitiv

100

60
40

4.3

4.4

4.5

4.6

4.7

Pierce 2 GISNEGQNASIK

100

4.67

3.6x
1.7x
6.1x

80
60

4.67

40

5.0

5.1

5.2

Pierce 5 IGDYAGIK
5.22

100

5.54

60

5.54

5.3

2.1x
3.5x
7.2x

80
60
40

0
5.40

5.45

5.50

5.55

5.60

5.65

5.70

Pierce 8 ELGQSGVDTYLQTK

6.14

100

4.1x
1.6x
6.7x

80
60

6.14

40

5.22

4.67

20

4.9

3.2x
1.4x
4.4x

80

20

5.11

0
4.2

5.54

100

40

5.11

20

0

4.6x
2.9x
13.2x

5.11

80

20

0

5.22

6.15

20

0
4.5

4.6

4.7

4.8

4.9

5.1

Pierce 3 HVLTSIGEK

100

60
40

4.91

100

1.1x
3.4x
3.6x

4.91
80

5.2

5.3

5.4

6.00

Pierce 6 TASEFDSAIAQDK

5.50

60
40

5.51

20

5.50

6.10

6.20

6.25

6.30

6.40

100

4.5x
2.6x
11.6x

80
60
40

6.40

4.91

20

6.15

Pierce 11 SFANQPLEVVYSK

3.7x
2.3x
8.6x

80

6.05

20

6.40

0
4.7

4.8

4.9

5.0

Pierce 4 DIPVPKPKColumns:
Part Nos.:
Eluent:Gradient:
Flow rate:
Temperature:
Detection:
Injection:
Sample:System:

5.1

5.2

5.40

5.45

5.50

5.55

Pierce 7 SAAGAFGPELSR

5.60

6.25

6.30

6.35

6.40

6.45

6.50

6.55

Pierce 9 GLILVGGYGTR

YMC-Triart C18 (1.9 µm, 12 nm) 150 x 2.1 mm ID
YMC-Triart C18 (1.9 µm, 12 nm) 150 x 1.0 mm ID
YMC-Triart C18 (1.9 µm, 12 nm) 150 x 0.5 mm ID
TA12SP9-15Q1PT
TA12SP9-1501WTTA12SP9-15J0AU
Water + 0.1% formic acid
Acetonitrile + 0.1% formic acid
10–100%B (0–18 min)
350 µL/min for 2.1 mm ID
80 µL/min for 1.0 mm ID
20 µL/min for 0.5 mm ID
30 °C
ESI positive
1 µL (500 fmol)
0.5 µL (plasma)
Pierce™ Peptide mix (ThermoFisher)
Pooled human plasma (protein precipitated) in 40% methanol
Modified Waters I-class UPLCTIC chromatograms of a peptide mix using YMC-Triart C18 columns of different internal diameters:
2.1, 1.0 and 0.5 mm. A consequent sensitivity gain is achieved with the reduction of column internal diameter. Peak areas achieved with a 0.5 mm ID MicroLC column are up to 3.5 times higher
compared to a 1.0 mm ID UHPLC column and even up to 13 times higher compared to a typical
UHPLC column with 2.1 mm ID. The selectivity remains the same. Therefore, the optimum ID caneasily be chosen dependent on the available sample amount and chromatographic setup in the lab [2].

8MicroLC applications

Untargeted screening of a protein precipitated plasma sample

63% more ted
es detec
t
i
l
o
b
a
t
e
m

The increased sensitivity by the use of a MicroLC column is especially useful for untargeted profiling with limited sample amounts such as in human plasma. In this example, 63% more metabolites
were detected than with the classical setup (not shown) [2].
Application data by courtesy of Sergey Girel, Institute of Pharmaceutical Sciences of Western Switzerland (University of Geneva), Geneva, Switzerland.

2.1 mm ID➔ 1.0 mm ID ➔ 0.5 mm ID[2] Sergey Girel, Víctor González-Ruiz, Serge Rudaz, Operating regular LC in microflow mode
to enhance ensitivity and metabolome coverage, Poster (SGMS annual meeting), 2022.

9

Capillary Columns

Commercial monoclonal
antibodies by MicroLC-MS
In cooperation with Shimadzu Europa

Rituximab (MabThera®)
TIC
dosage form

100.00

%

0.00

0

1

2

3

4

5

6

7

8

9

10

11

12

13

15
RT (min)Deconvoluted
spectrum

147240.67188
1.6e5

14

147402.67188

1.4e5
147079.29688

1.2e5
1.0e5
8.0e4

147565.79688

147336.53125

6.0e4

147503.87500

147174.71875

4.0e4

0.0e0

Column:Part No.:
Eluent:146938.54688

1.464e51.464e51.465e51.465e51.466e51.466e51.467e51.467e51.468e51.468e51.469e51.469e5

1.47e5147780.25000

147011.90625

1.47e51.471e51.471e5

YMC-Triart Bio C4 (30 nm, 3 μm) 100 x 0.3 mm ID, 1/16" end fittings
TB30S03-10H0AU
A) H2O + 0.1 % formic acidB) acetonitrile + 0.1% formic acid
Gradient:
20 %B (0–2.5 min), 20 %–100 %B (2.5–4 min), 100%B (4–5 min), 20 %B (5–7 min)Flow rate:
15 μL / minTemperature: 75 °C, 4 °C Autosampler
Detection:
Shimadzu LCMS-9030 QTOF
Injection:
0.1 μL
Sample:Rituximab dosage form (10 mg/mL, diluted to 0.1 μg/mL)
LC system:
Shimadzu Nexera Mikros

10147670.17188

147463.03125

2.0e4

1.472e51.472e51.473e51.473e51.474e51.474e51.475e51.475e51.476e51.476e51.477e51.477e51.478e5MassMicroLC applications

Bevacizumab (Avastin®)

TIC
dosage form

100.00

%

0.00

0

1

2

3

4

5

6

7

8

9

10

11

RT (min)TIC
aged solution for patient application

100.00

%

0.00

0

1

2

3

4

5

6

7

8

9

10

11

RT (min)Deconvoluted
spectrum149201.78125

6.0e5

5.0e5

4.0e5

3.0e5

149363.43750

2.0e5

149301.57813

1.0e5

0.0e0

148997.62500

1.49e51.49e51.49e5

1.491e51.491e51.491e51.491e51.491e51.492e51.492e51.492e51.492e51.492e51.493e51.493e51.493e51.493e51.493e51.494e51.494e51.494e51.494e5149525.14063

149464.79688

149418.59375

149107.70313

149060.25000
1.49e51.494e51.495e51.495e51.495e51.495e5

Mass

Column:Part No.:
Eluent:YMC-Triart Bio C4 (30 nm, 3 μm) 100 x 0.3 mm ID, 1/16" end fittings
TB30S03-10H0AU
A) H2O + 0.1% formic acidB) acetonitrile + 0.1% formic acid
Gradient:
20 %B (0–2.5 min), 20 %–100 %B (2.5–4 min), 100 %B (4–5 min), 20 %B (5–7 min)Flow rate:
15 μL / minTemperature: 75 °C, 4 °C Autosampler
Detection:
Shimadzu LCMS-9030 QTOF
Injection:
0.1 μL
Sample:Bevacizumab dosage form (10 mg/mL, diluted to 0.1 μg/mL)
Bevacizumab solution for patient application (3 mg/mL, diluted to 0.03 μg/mL)
LC system:
Shimadzu Nexera Mikros

Chromatograms of intact monoclonal antibodies, Rituximab and Bevacizumab, both in dosage form.
The aged solution (about 6 month old) for patient application showed an additional peak, but themAb is still intact. This demonstrates that use of MicroLC-MS is possible to monitor amount andstability of, for example, patient solutions for injection.

11Capillary Columns

Gluten markers in flour and cookies
MicroLC-MS/MS analysis of gluten markers in flour

Column:Part No.:
Eluent:YMC-Triart C18 (12 nm, 3 µm) 100 x 0.5 mm ID, 1/32" end fittings
TA12S03-10J0RUA) H2O + 0.1% formic acidB) acetonitrile + 0.1% formic acid
Gradient:
5%B (0–1 min), 25%B (6 min), 95%B (8–9 min), 5%B (9.2–12 min)Flow rate:
25 µL/min
Temperature: 40 °C
Detection:
SCIEX 5500 QTRAP, ESI
Injection:
10 µL
LC system:
Eksigent ekspert MicroLC 200

The comparison of separate extracts of several samples of wheat obtained from single variety grain
samples, as well as a sample of gluten free flour and the self raising flour obtained from a local
supermarket.

12MicroLC applications

MicroLC-MS/MS analysis of wheat or oat markers for gluten in cookies

freeGlutenfree
Gluten&Wheat rsrke
o at m aheat
Only w s
marker

It is possible to detect markers in processed food and also distinguish between varieties. In the oat
cookies, wheat and oats markers were detected while in the wheat cookies only wheat peptide markers were found. The gluten free products were actually gluten free, as no markers where detectable.
Calibration of gliadin

The calibration line obtained from the spiking of gliadin, a specific wheat protein, into gluten free
wheat from the range of 5–200 ppm. Inlayed in the calibration line is the chromatogram for the 10 ppm
spike of gliadin into gluten free flour.
Application data by courtesy of: Stephen Lock, SCIEX, Warrington (UK)13

Capillary Columns

Allergens in wine
Casein peptide analysis in white wine by HPLC and MicroLC

conventional HPLC

MicroLCYMC-Triart C18

:
y
t
i
v
i
t
i
s
sen
>8 fold+

runtime-50 %Columns:

YMC-Triart C18 (12 nm, 3 μm) 50 x 0.5 mm ID, 1/32" end fittings (MicroLC)
Kinetex XB-C18 (10 nm, 2.6 μm) 50 x 2.1 mm ID (HPLC)TA12S03-05J0RUA) H2O + 0.1% formic acidB) acetonitrile + 0.1% formic acid
HPLC Gradient:
2%B (0–2 min), 60%B (8 min), 95%B (8.2–9 min), 2%B (9.1–10 min)MicroLC Gradient: 2%B (0–0.3 min), 40%B (4 min), 95%B (4.1–4.3 min), 2%B (4.4–5.5 min)Flow rate:
300 μl/min (HPLC)
25 μl/min (MicroLC)
Temperature:
40 °C
Detection:
SCIEX QTRAP 5500, ESI
Injection:
10 μl
LC-System:
Shimadzu Nexera XR (HPLC)
Eksigent ekspert MicroLC 200
Part No.:
Eluent:Comparison of HPLC vs. MicroLC using a white wine spiked with 1 ppm casein peptide. By using
an optimised MicroLC method the sensitivity can be increased by >8 fold, while the runtime canbe reduced by 50%.

14

MicroLC applications

Screening of allergens in white wine by MicroLC-MS/MS

Casein pe

ptide

ide
Egg pept

Blanc
n
o
n
g
i
v
u
Sa
la
Chenin B

nc

nay
ChardonWhite wine samples spiked with 0.5 ppm samples of milk and egg peptides were analysed.
3 different wines were analysed using the benefit of the MicroLC-MS/MS approach with theadditional advantage of a multi allergen screening.

A casein peptide is spiked into a Sauvignon Blanc (0.05–2 ppm) to demonstrate linearity andsensitivity. Linearity is provided without use of any internal standards. The inset chromatogram for
50 ppb spiked sample demonstrates highest sensitivity.
Application data by courtesy of: Stephen Lock, SCIEX, Warrington (UK)15

Capillary Columns

Veterinary drug residues in food
Veterinary drug standards analysed by HPLC and MicroLC

Enhanced method
MicroLC, YMC-Triart C18

Dicloxacillin

Ampicillin

SulfadiazineStandard method
conventional HPLC

runtime- 65 %

Columns:

YMC-Triart C18 (12 nm, 3 μm) 50 x 0.5 mm ID, 1/32" end fittings (MicroLC)
Kinetex XB-C18 (10 nm, 2.6 μm) 50 x 2.1 mm ID (HPLC)TA12S03-05J0RUA) H2O + 0.1% formic acidB) acetonitrile + 0.1% formic acidEnhanced Gradient: 2%B (0–0.5 min), 65%B (1.7 min), 100%B (1.8–2.3 min), 2%B (2.4–3.5 min)Flow rate:
600 μl/min (HPLC)
30 μl/min (MicroLC)
Temperature:
60 °C
Detection:
SCIEX QTRAP 5500, ESI
Injection:
2 μl
LC-System:
Shimadzu Nexera XR (HPLC)
Eksigent ekspert MicroLC 200
Part No.:
Eluent:Comparison of 3 different 1 ppb standard solutions separated by a standard HPLC method using
a Core-Shell C18 column (left) and the MicroLC method using a YMC-Triart C18 MicroLC column(right).

16

MicroLC applications

MicroLC-MS/MS analysis of 15 different veterinary drugs in milk and meat

In the final analysis a total of 32 multiple reaction monitoring (MRM) transitions were evaluated for
15 veterinary drug residues over a 3.5 minute runtime on the YMC-Triart C18 column. Milk and meat
samples have been spiked at a 10 ppb level with standard compounds. The recoveries from meat weregenerally higher and it shows that recoveries are affected by the matrix.

Ampicillin calibration curve

Linearity and sensitivity of this method is demonstrated for Ampicillin from 0.05–50 ppb. Linearity isprovided without use of any internal standards. The inset chromatogram for a 0.5 ppb spiked sample
demonstrates the high level of sensitivity.
Application data by courtesy of: Stephen Lock, SCIEX, Warrington (UK)17

Capillary Columns

Solutions for small molecules

Phase overview YMC-Triart
YMC-Triart C18

YMC-Triart C18 ExRSYMC-Triart Bio C18

YMC-Triart C8

Si

Si

Si

Si

C18

C18ExRS

Bio C18

C8

versatile applicationshydrophobic substances
positional isomers

peptides/proteins/oligonucleotides

alternative to C18

first choice formethod development

300 Å widepore

extended pH and stability

short retention time

pH 1–12/90 °C max.

100% aqueous eluentsYMC-Triart Phenyl

YMC-Triart PFP
F

F

Si

OH

HO

OH

HO

OH

Si

OH

HO

OH

Bio C4

PhenylPFP

HILIC

proteins/antibodies/peptides

aromatic compounds(π-electron donor)

aromatic compounds(π-electron donor)

very polar compounds

300 Å widepore
pH 1–10/90 °C max.
100% aqueous eluents

18

OH

F
HO

HO

HO

Si

YMC-Triart Diol-HILIC

F

F
Si

pH 1–12/90 °C max.

OH

YMC-Triart Bio C4

pH 1–12/90 °C max.

OH

100% aqueous eluents

pH 1–12/90 °C max.

conjugated systems
100% aqueous eluents

cis-trans isomers

less ionic adsorption

polar halogenatedcompounds

ideal choice for SFC

100% aqueous eluents

100% aqueous eluents

Solutions for small molecules

Specification YMC-Triart
C18

C18 ExRS

Bio C18

Base

C8

Bio C4

PhenylPFP

Diol-HILIC

Phenyl(USP L11)

Pentafluorophenyl(USP L43)

Diol(USP L20)

organic/inorganic hybrid silica

Stationary
phase

C18
(USP L1)

C18(USP L1)

C18(USP L1)

Particle size

C8
(USP L7)

C4(USP L26)

1.9, 3 and 5 µm

Pore size

12 nm

8 nm

30 nm

12 nm

30 nm

12 nm

12 nm

12 nm

Specific surface

360 m2/g

430 m2/g—

360 m2/g—

360 m2/g

360 m2/g

360 m2/g

Carbon content

20%

25%

—

17%

—

17%

15%

—

Bonding

trifunctional

Endcapping

multi-stage

multi-stage

multi-stage

multi-stage

multi-stage

multi-stage

none

none

pH range

1 ~ 12

1 ~ 12

1 ~ 12

1 ~ 12

1 ~ 101 ~ 101~8

2 ~ 10

50 °C

50 °C

50 °C

✓

✓

✓

Temperaturerange

pH < 7: 90 °C pH < 7: 90 °C pH < 9: 90 °C pH < 7: 90 °C pH < 7: 90 °C
pH > 7: 50 °C pH > 7: 50 °C pH > 9: 50 °C pH > 7: 50 °C pH > 7: 50 °C

100%aqueous eluents

✓

✘

✓

✘

✓

First choice for method development

Hydrogen bonding capacity
α (caffeine/benzene)

Hydrophilic ODS
Hydrosphere C18

Pro C8

Standard ODS
Pro C18

Triart C8 Triart
C18
Symmetry C18
ZORBAX Extend-C18

Hydrophobic ODS
Pro C18 RS

Triart C18 ExRS
16.00
Hydrophobicity k‘ (amylbenzene)

19Capillary Columns

Columns for biomolecules

BioLC phase selection
Column Selection Tool according to molecular weight
MW

5,000

10,00020,00030,00070,000100,000

150,000

YMC-Triart C18

YMC-Triart Bio C18

Hydrosphere
C18

most appropriate MW range

YMC-Triart Bio C4

extended MW range by elevated temperatureappropriate MW range

Selectivities for proteins/peptides and antibodies
YMC-Triart
Bio C4

Particle Size / µm

C4 (USP L26)

C18 (USP L1)

C18 (USP L1)

1.9, 3, 5

1.9, 3, 5

1.9, 3, 5

30

12

30

1.0–10.0

1.0–12.0

1.0–12.0

pH < 7: 90 °C
pH > 7: 50 °C

pH < 7: 90 °C
pH > 7: 50 °C

pH < 9: 90 °C
pH > 9: 50 °C

Pore Size / nm
pH range
Temperature range

YMC-Triart
Bio C18

organic/inorganic hybrid silica

Base particle
Modification

YMC-Triart
C18

Selectivities for oligonucleotides
YMC-Triart
C18

Particle Size / µm
Pore Size / nm
pH range
Temperature range

20

YMC-Triart
C8organic/inorganic hybrid silica

Base particle
Modification

YMC-Triart
Bio C18

Hydrosphere
C18
silicaC18 (USP L1)

C18 (USP L1)

C8 (USP L7)

C18 (USP L1)

1.9, 3, 5

1.9, 3, 5

1.9, 3, 5

2, 3, 5

12

30

12

12

1.0 – 12.0

1.0–12.0

1.0–12.0

2.0–8.0

pH < 7: 90 °C
pH > 7: 50 °C

pH < 9: 90 °C
pH > 9: 50 °C

pH < 7: 90 °C
pH > 7: 50 °C

50 °C

Columns for biomolecules

BioLC selectivities

Hydrogen bonding capacitya (caffeine/benzene)

Ideal selectivity for biomolecules

Hydrophobicity k'(Amylbenzene)

YMC-Triart Bio C18 shows a selectivity between that of YMC-Triart Bio C4 and regular YMC-Triart   C18/C8
with 12 nm pore. This feature perfectly supplements the existing products.

Hydrogen bonding capacitya (caffeine/benzene)

Low hydrophobicity and hydrogen capacity

Triart Bio C4

YMC-Triart Bio C4 is based on the robust, well-established organic/hybrid particle. It provides lowhydrophobicity and lower hydrogen bonding capacity compared to other BioLC columns.

21

Capillary Columns

Column hardware

YMC capillary column hardware• All fully porous YMC phases available• Compatible with several Nano-/MicroLC/MS systems, e.g. from Eksigent/Sciex (M5/ExpressLC ultra/
Ekspert MicroLC 200), Shimadzu (Nexera Mikros), Waters ACQUITY UPLC M-Class• Suitable for extremely low sample volumes and low flow rates• Fused silica inner body
• Stainless steel outer body used for MicroLC columns (0.3/0.5 mm ID)• Polymer coating as outer body used for NanoLC columns (0.1/0.075 mm ID)• Optionally fully bioinert microLC hardware available (0.3/0.5 mm ID)
Dimensions
IDs [µm]

Lengths [mm]Particle sizes [µm]*

500

50

1.9

300

75

2.0

100

100

3.0

75

150

5.0

*Not all dimension/particle size combinations are possible. · Other dimensions are available on request.

Connection options
All columns are available with 2 connection options:
1/16" connections (10–32 thread),e.g. for Shimadzu Nexera Mikros or Waters ACQUITY UPLC M-Class
1/32" connections (6–40 thread) for Eksigent/Sciex

Pressure limits
Pressure stability of the column is dependent on particle size:
2–5 μm: 55 MPa/ 7,975 psi (45 MPa / 6,525 psi for 75 / 100 µm ID)
1.9 μm: 60 MPa / 8,700 psi.

22

Column hardware

Typical flow rates
IDs [µm]

Typical flow rates for RP*

500

10–30 μL/min

300

3–10 μL/min

100

300–500 nL/min

75

100–300 nL/min*Depending on length and particle size. In HILIC mode it is recommended flow rates should be reduced by half.

Guard/trap columns

Supplied in packs of 3 together with a column coupler.
Suitable for:
 guard columns for challenging matrices (with column coupler) trapping columns for use in desalting or trap and elute procedures (without a coupler)

Column coupler

1/16" coupler (P/N XRCP1601E-1PK)

1/32" coupler (P/N XRCP3201E-1PK)

A custom-made stainless-steel column coupler is supplied with every pack of 3 capillary guard cartridges toguarantee the optimum connection with low dead volume. Every coupler can be purchased separately, if required.

1/32" guard column coupled to a 1/32" analytical column.

23

Capillary Columns

Ordering information

Part numbers for YMC-Triart columns
YMC capillary columns are available with 1/16" (10–32 thread) or with 1/32" (6–40 thread) connections.
All column part numbers indicate the connection size by use of additional terminal letters:
columns with 1/16" fittings end with -AUcolumns with 1/32" fittings end with -RU
bioinert columns with 1/16" fitting end with -AUC

Order codes YMC-Triart columns
PLEASE NOTE:Part numbers for 1/16" connections are shown.
To order 1/32" connections or bioinert 1/16" columns, simply exchange AU by RU or AUC,
respectively.
Columns with 1/32" connection as well as bioinert columns are available with 300 µm or
500 µm ID.

YMC-Triart 1.9 µm, 1/16" | 1/32" fitting, MicroLC capillary columns (max. pressure 60 MPa)
Phase

ColumnID[µm]

Column length [mm]Guard columns*
with 5 mm
length

50

75

100

150

(pack of 3)

C18

300
500

TA12SP9-05H0AU
TA12SP9-05J0AU

TA12SP9-L5H0AU
TA12SP9-L5J0AU

TA12SP9-10H0AU
TA12SP9-10J0AU

TA12SP9-15H0AU
TA12SP9-15J0AU

TA12SP9-E5H0AU
TA12SP9-E5J0AU

C18 ExRS

300
500

TAR08SP9-05H0AU
TAR08SP9-05J0AU

TAR08SP9-L5H0AU
TAR08SP9-L5J0AU

TAR08SP9-10H0AU
TAR08SP9-10J0AU

TAR08SP9-15H0AU
TAR08SP9-15J0AU

TAR08SP9-E5H0AU
TAR08SP9-E5J0AU

Bio C18300
500

TA30SP9-05H0AU
TA30SP9-05J0AU

TA30SP9-L5H0AU
TA30SP9-L5J0AU

TA30SP9-10H0AU
TA30SP9-10J0AU

TA30SP9-15H0AU
TA30SP9-15J0AU

TA30SP9-E5H0AU
TA308SP9-E5J0AU

C8

300
500

TO12SP9-05H0AU
TO12SP9-05J0AU

TO12SP9-L5H0AU
TO12SP9-L5J0AU

TO12SP9-10H0AU
TO12SP9-10J0AU

TO12SP9-15H0AU
TO12SP9-15J0AU

TO12SP9-E5H0AU
TO12SP9-E5J0AU

Bio C4300
500

TB30SP9-05H0AU
TB30SP9-05J0AU

TB30SP9-L5H0AU
TB30SP9-L5J0AU

TB30SP9-10H0AU
TB30SP9-10J0AU

TB30SP9-15H0AU
TB30SP9-15J0AU

TB30SP9-E5H0AU
TB30SP9-E5J0AU

Phenyl300
500

TPH12SP9-05H0AU
TPH12SP9-05J0AU

TPH12SP9-L5H0AU
TPH12SP9-L5J0AU

TPH12SP9-10H0AU
TPH12SP9-10J0AU

TPH12SP9-15H0AU
TPH12SP9-15J0AU

TPH12SP9-E5H0AU
TPH12SP9-E5J0AU

PFP

300
500

TPF12SP9-05H0AU
TPF12SP9-05J0AU

TPF12SP9-L5H0AU
TPF12SP9-L5J0AU

TPF12SP9-10H0AU
TPF12SP9-10J0AU

TPF12SP9-15H0AU
TPF12SP9-15J0AU

TPF12SP9-E5H0AU
TPF12SP9-E5J0AU

Diol-HILIC

300
500

TDH12SP9-05H0AU
TDH12SP9-05J0AU

TDH12SP9-L5H0AU
TDH12SP9-L5J0AU

TDH12SP9-10H0AU
TDH12SP9-10J0AU

TDH12SP9-15H0AU
TDH12SP9-15J0AU

TDH12SP9-E5H0AU
TDH12SP9-E5J0AU* no holder required, comes with a column coupler

24Ordering information

YMC-Triart 3 µm, 1/16"|1/32" fitting, MicroLC capillary columns (max. pressure 55 / 45 MPa)
Phase

Column
ID
[µm]

75

C18

C18 ExRS

Bio C18

C8

Bio C4

PhenylPFP

Diol-HILIC

Column length [mm]Guard columns*
with 5 mm
length

50

75

100

150

(pack of 3)——

TA12S03-10E8AU

TA12S03-15E8AU

—

100

—

—

TA12S03-10F0AU

TA12S03-15F0AU—

300

TA12S03-05H0AU

TA12S03-L5H0AU

TA12S03-10H0AU

TA12S03-15H0AU

TA12S03-E5H0AU

500

TA12S03-05J0AU

TA12S03-L5J0AU

TA12S03-10J0AU

TA12S03-15J0AU

TA12S03-E5J0AU

75

—

—

TAR08S03-10E8AU

TAR08S03-15E8AU

—

100

—

—

TAR08S03-10F0AU

TAR08S03-15F0AU—

300

TAR08S03-05H0AU

TAR08S03-L5H0AU

TAR08S03-10H0AU

TAR08S03-15H0AU

TAR08S03-E5H0AU

500

TAR08S03-05J0AU

TAR08S03-L5J0AU

TAR08S03-10J0AU

TAR08S03-15J0AU

TAR08S03-E5J0AU

75

—

—

TA30S03-10E8AU

TA30S03-15E8AU

—

100

—

—

TA30S03-10F0AU

TA30S03-15F0AU—

300

TA30S03-05H0AU

TA30S03-L5H0AU

TA30S03-10H0AU

TA30S03-15H0AU

TA30S03-E5H0AU

500

TA30S03-05J0AU

TA30S03-L5J0AU

TA30S03-10J0AU

TA30S03-15J0AU

TA30S03-E5J0AU

75

—

—

TO12S03-10E8AU

TO12S03-15E8AU

—

100

—

—

TO12S03-10F0AU

TO12S03-15F0AU—

300

TO12S03-05H0AU

TO12S03-L5H0AU

TO12S03-10H0AU

TO12S03-15H0AU

TO12S03-E5H0AU

500

TO12S03-05J0AU

TO12S03-L5J0AU

TO12S03-10J0AU

TO12S03-15J0AU

TO12S03-E5J0AU
—

75

—

—

TB30S03-10E8AU

TB30S03-15E8AU

100

—

—

TB30S03-10F0AU

TB30S03-15F0AU—

300

TB30S03-05H0AU

TB30S03-L5H0AU

TB30S03-10H0AU

TB30S03-15H0AU

TB30S03-E5H0AU

500

TB30S03-05J0AU

TB30S03-L5J0AU

TB30S03-10J0AU

TB30S03-15J0AU

TB30S03-E5J0AU

75

—

—

TPH12S03-10E8AU

TPH12S03-15E8AU

—

100

—

—

TPH12S03-10F0AU

TPH12S03-15F0AU—

300

TPH12S03-05H0AU

TPH12S03-L5H0AU

TPH12S03-10H0AU

TPH12S03-15H0AU

TPH12S03-E5H0AU

500

TPH12S03-05J0AU

TPH12S03-L5J0AU

TPH12S03-10J0AU

TPH12S03-15J0AU

TPH12S03-E5J0AU

75

—

—

TPF12S03-10E8AU

TPF12S03-15E8AU

—

100

—

—

TPF12S03-10F0AU

TPF12S03-15F0AU—

300

TPF12S03-05H0AU

TPF12S03-L5H0AU

TPF12S03-10H0AU

TPF12S03-15H0AU

TPF12S03-E5H0AU

500

TPF12S03-05J0AU

TPF12S03-L5J0AU

TPF12S03-10J0AU

TPF12S03-15J0AU

TPF12S03-E5J0AU

75

—

—

TDH12S03-10E8AU

TDH12S03-15E8AU

—

100

—

—

TDH12S03-10F0AU

TDH12S03-15F0AU—

300

TDH12S03-05H0AU

TDH12S03-L5H0AU

TDH12S03-10H0AU

TDH12S03-15H0AU

TDH12S03-E5H0AU

500

TDH12S03-05J0AU

TDH12S03-L5J0AU

TDH12S03-10J0AU

TDH12S03-15J0AU

TDH12S03-E5J0AU* no holder required, comes with a column coupler

PLEASE NOTE:For use with Ekisgent/Sciex MicroLC systems, order columns with 1/32" (6–40 thread) end-fittings.
Use the following tube connectors, Eksigent 6/40 Fitting P/N 5019621 or VICI No Twist One-Piece
Fitting P/N ZNF.5FPK(-5) or Optimize EXP®2 Fitting Kit P/N 15-19-04927.

25

Capillary Columns

Part numbers for further YMC columns
The specific part number for a given column consists of two parts describing the phaseand dimension/hardware details:

Phase – Dimension/hardware
Both parts of the part number can be taken from the tables below:
A selection of YMC RP phases

A selection of YMC HILIC / NP phases(first part of part number)(first part of part number)

Phase

Particle size
[µm]

Phase

Particle size[µm]Part number

YMC-Pack SIL, 12 nm

3

SL12S03-

YMC-Pack PVA-Sil, 12 nm

5

PV12S05-

Part number

2

AS12S02-3

AS12S03-2

HS12S02-3

HS12S03-

YMC-Pack Polyamine II, 12 nm

5

PB12S05-

YMC-Pack Pro C18 RS, 8 nm

3

RS08S03-

YMC-Pack NH2, 12 nm

3

NH12S03-

YMC-Pack Pro C8, 12 nm

3

OS12S03-

YMC-Pack Diol (NP),12 nm

3

DN12S03-

YMC-Pack Pro C4, 12 nm

3

BS12S03-

YMC-Pack CN, 12 nm

3

CN12S03-

YMC-Pack Pro C18, 12 nm
Hydrosphere C18, 12 nm

A selection of YMC SEC phases(first part of part number)
Phase

Particle size[µm]Part number

3

DLM25S03-

3
5
3
5
2
3
5
2
3
5

DL06S03DL06S05DL12S03DL12S05DL20S02DL20S05DL20S05DL30S02DL30S05DL30S05-

YMC-SEC MAB, 25 nm
YMC-Pack Diol-60, 6 nm
YMC-Pack Diol-120, 12 nm
YMC-Pack Diol-200, 20 nm

YMC-Pack Diol-300, 30 nm

EXAMPLE:YMC-Pack Pro C18, 12 nm, 3 µm
100 mm x 300 µm
1/16" end fittings
First part:
AS12S03Second part: 		-10H0AU
Part number:

AS12S03 -10H0AU

Further particle sizes (e.g. 5 µm) or stationary phases (e.g. CHIRAL ART) available on request.
Dimension/hardware selection (second part of part number)* (max. pressure 55 / 45 MPa)
Fitting

1/16"

1/32"

ColumnID[µm]

Column length [mm]

50

75

100

150

500

-05J0AU-L5J0AU-10J0AU-15J0AU-E5J0AU

300-05H0AU-L5H0AU-10H0AU-15H0AU-E5H0AU100––-10F0AU-15F0AU

–

75

–

–

-10E8AU-15E8AU–

500-05J0RU-L5J0RU-10J0RU-15J0RU-E5J0RU

300-05H0RU-L5H0RU-10H0RU-15H0RU-E5H0RU*Not all phase and hardware combinations might be possible.

26

Guard columns
with 5 mm
length(pack of 3)

Please request the following catalogues(U)HPLC columns
YMC-Triart

YMC Biochromatography
Columns

BioPro IEX Resins

YMC Chiral
Columns

YMC-Triart Prep

The following brands, trademarks, or service marks are the property of the listed company and/or its subsidiaries.
Every effort has been taken to ensure this list is accurate at the time of printing this brochure.
Capcell Pak is a trademark of Shiseido Irica Technology Inc.
Inertsil is a trademark of GL Sciences Inc.
ACQUITY, Atlantis, BEH, BioResolve, CSH, SunFire, Symmetry, XBridge, XTerra are trademarks of Waters Corp.
Aeris, Gemini, Kinetex are trademarks of Phenomenex Inc.
Hypersil GOLD is a trademark of Thermo Fisher Scientific Inc.
Kromasil is a trademark of Nouryon.
AdvanceBio, Zorbax are trademarks of Agilent Technologies Inc.
L-column is a trademark of Chemicals Evaluation and Research Institute.
Ascentis, Express are trademarks of Merck KGaA.

27

Your local contact:

YMC CO., LTD.
www.ymc.co.jp

YMC Europe GmbHwww.ymc.eu

YMC America, Inc.
www.ymcamerica.com

YMC Switzerland LLC
www.ymc-schweiz.ch

YMC India Pvt. Ltd.
www.ymcindia.com

YMC Shanghai Rep. Officewww.ymcchina.com

YMC Korea Co., Ltd.
www.ymckorea.com

YMC Taiwan Co., Ltd.
www.ymctaiwan.com

YMC Singapore Tradelinks Pte. Ltd.
www.ymc.sg

YMC Capillary Columns_05/2024_A4int_1.0