Features of PentaFluoroPhenyl group bonded column - YMC-Triart PFP －Effective for separation of polar compounds or isomers provided by unique polar interaction－F130705AE

Features and specifications of YMC-Triart PFP
YMC-Triart PFP is a pentafluorophenyl group bonded phase. YMC-Triart PFP has unique selectivity provided by various interactions
such as π-π, and dipole-dipole as well as hydrophobic. YMC-Triart PFP is effective especially for improving separation of aromaticcompounds, nitro compounds, and compounds with halogen because its selectivity is very different from other chemistries.
YMC-Triart PFP

Image structure for Triart PFP

FFPentafluorophenyl group

δ－FRN

δ＋・π-π interaction

FFF

Particle size

1.9 µm, 3 µm, 5 µm

Pore size

12 nmRBonding

PolymericEndcapping

NopH range

1~8

Usable temperature
range (upper limit)

50℃

100% aqueouscompatibility

Yes

USP Classification

L43

Especially effective for;・aromatic compounds

1Non-endcapping・compounds with halogen

SiHO

Organic / inorganic hybrid silica

δ－ δ＋3HOBase

Unique interaction of PFP
phase recognizes minor
charge localization in amolecule2・dipole-dipole interaction

Pentafluorophenylpropyl

RR・strong dipole-dipole interaction

Functional group・isomers

OOOHHOOH

Organic / inorganic hybrid silica

Effective for separation of isomers
Piperine cis-trans isomers
HH

1OH

2O
H

H
HO

N

3OO

N
O

Isopiperine
Z,E-(cis-trans)-piperine

Rs (1,2) = 1.19
Rs (2,3) = 1.54

Triart PFP

Good resolution

3

24F121213E

1, 2, 4
3

Triart C18F121214C

1, 2, 4
3

Triart C8F121214G

1, 2, 4

Triart Phenyl

3F121214D

1, 2, 4

Silica based3

Phenyl-HexylF121217B

HPiperine
E,E-(trans-trans)-piperine

Even though the difference inhydrophobicity of cis-trans isomers ofpiperine, which is a pungent
component contained in pepper, isvery small, YMC-Triart PFP canseparate them very well.
It is considered that YMC-Triart PFP
has high recognition ability of minor
charge localization in a molecule
provided by various interactions such
as π-π and dipole-dipole as well ashydrophobicity. Such feature greatlycontributes to excellent selectivity ofstructurally similar compounds
Under this conditions, Triart PFP
showed much better separation than aconventional silica based PFP column.

1, 23Silica basedColumn
EluentFlow rate

4PFP
F121213C0ON

H

N

Isochavicine
E,Z-(trans-cis)-piperine

1HO

H

H

Chavicine
Z,Z-(cis-cis)-piperine

4HO

HO

HH

O

H

O

51015

20

25

min

http://www.ymc.co.jp: 5 µm, 150 X 3.0 mmI.D. or 150 X 4.6 mmI.D.
: acetonitrile/0.1% formic acid (40/60): 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
Temperature : 25ºCDetection: UV at 280 nm

Simultaneous analysis of 14 biologically active polar compounds
Catecholamines, serotonin and their precursors and metabolites
123OH

4OH

H2N

OH

OH

HOOH

・HClNH2OH

Noradrenaline HCl (NA)
(Norepinephrine HCl)

3,4-Dihydroxymandelicacid (DOMA)

89

NH2OH

3,4-Dihydroxyphenylalanine(DOPA)
10

OH

HO

HOO

OH

OH

OH

・HClOH

HOO
CH3・HCl

Dopamine HCl (DA)

1112

13

H
N

NH2H
N

O

HOO

OH

Adrenaline HCl (A)(Epinephrine HCl)

CH3・HClO

OH

O

CH3OH

NH

OH

Tyrosine (Tyr)

OH

H2N

7H2N

H3C

O

O

6OH

OH
OOH5OHOH

Vanillylmandelicacid (VMA)

HN

14HOO

HOH2N

CH3・HClOHOOOHOHOH

3-Methoxy-4-hydroxy
phenylglycol (MHPG)

3,4-Dihydroxyphenylacetic
acid (DOPAC)

3-Methoxytyramine HCl(3MT)

5-Hydroxytryptamine HCl (5HT)(Serotonin HCl)

Tryptophan (Trp)5-Hydroxyindoleacetic
acid (5HIAA)

Homovanillicacid (HVA)

Comparison of separation selectivity among YMC-Triart RP columns
Acidic compounds

5 µm, 150 X 3.0 mmI.D.
1131211910874PFP

1413. 5HIAA

5623Triart

14. HVAGood resolution
with long retention

9. DOPAC

C18C813

2. DOMA

Phenyl870.0

144C1810236115Triart

PFP

7. VMA

9 ＋ 121F130430A

10.0

20.0

30.0

40.0

Time (min)F130424B

1Neutral and zwitterionic compounds
1113127

8. MHPG

149C884103(zwitterionic)

2 65Triart

12. Trp(neutral)

F130425B

4(zwitterionic)

PFP
C18

3. DOPA
148C8(zwitterionic)

13102

Phenyl

9 ＋ 12

7 ＋ 111536Triart

4. Tyr0.0

Phenyl
5.0

10.0

15.0

20.0

25.0

Time(min)
F130426A05101520253035Separation selectivity of YMC-Triart RP columns is compared on analysis of 14 biologically
active amines and their related compounds. Retention time of each compound issummarized by type of compounds. As shown, Triart PFP column shows strong retentionof basic compounds. It is considered that basic compounds which has electron-donating
characteristic and polarised PFP group/silanol group are strongly interacted, and as aresult, Triart PFP shows longer retention time.
Unique selectivity of Triart PFP provided by polar interactions contributes to separation ofpolar compounds which are not separated well with other functional groups. Triart PFP iseffective as a 2nd choice column for separation of polar compounds, especially in the casewhere good resolution is not achieved with C18 or other common RP phases.

Basic compoundsmin*11. 5HT10. 3MT

6. DAPFP

5. A

C18C8

1. NA

Phenyl
0.0

5.0

10.0

15.0

20.0

Time (min)3 µm, 150 X 3.0 mmI.D.
1 23Triart

Improved separation by 3 µm columnRs (2,3) = 1.54mAU

554PFP0611789 10131214F130425D05101520

By changing the size from 5 µm to 3 µm, separation of DOMA (peak 2) and DOPA(peak 3) is improved.

25ColumnEluent30: 150 X 3.0 mmI.D.
: A) 10 mM formic acidB) methanol containing 10 mM formic acid
0-20%B (0-30 min), 20%B (30-35 min)Flow rate: 0.425 mL/min
Temperature : 25ºC
Detection: UV at 280 nm

http://www.ymc.co.jpmin