YMC USER'S MANUAL

Column Care and Use Instructions

YMC CHIRAL NEA
YMC CHIRAL CD BR Series
YMC CHIRAL PREP CD Series
1. Introduction
Thank you for purchasing a YMC high-performance liquid chromatography (HPLC) column “YMC CHIRAL”. YMC CHIRAL series
are optical isomer separation columns. They are applicable for analysis of wide range of compounds by selecting the column for any
purpose.
YMC CHIRAL series, which are manufactured under highly controlled conditions, must pass a series of stringent tests before being
accepted for shipment. (Please refer to the column inspection report). To ensure optimal performance and durability of the column,
please read these instructions carefully before using this column.

2. Specifications
Packing material

Functional group

NEA

NEA (R)(R)- (naphthy)ethylamine

SeriesNEA (S)(S)- (naphthy)ethylamine

CD BR

α-CD BRα-bromo-cyclodextrin

Seriesβ-CD BRβ-bromo-cyclodextrin

γ-CD BRγ-bromo-cyclodextrin

PREP CDST

seriesβ-cyclodextrin

PM

Separation mode

UsablepH range

Usable temperature range
Regular use
Upper(recommended)
limit

*1

2 – 6.5

25 – 35 °C50 °Creversed phase

3.5 – 6.5

25 – 35 °C

50 °C

both phase(reversed phase isrecommended)

2–720 – 40 °C

50 °C

both phase *2*1 Regarding initial of product number, 〔NR、NS〕 is for reversed phase column, 〔CR、CS〕 is for normal phase column.
*2 Although both reversed phase and normal phase are available, we recommend using the same column exclusively for separation mode for column
lifetime.

3. Column connections
The “WT” or “PTH” at the end of the product code indicates the style of column endfittings.
WT = Waters style / PTH = Parker style

4. Shipping solvent
Indicated in the COLUMN INSPECTION REPORT. Replace with this solvent for storage.

5. Mobile phase【Reversed phase】
・ In general, acetonitrile, methanol and ethanol are recommended for regular use.
・ The mixture at an arbitrary ratio of organic solvent and aqueous solution of salt or water or buffer can be used. Recommended salt
concentrations of the mobile phase are as follows, NEA less than 1.0 M, CD BR less than 0.2 M, PREP CD less than 0.5 M.

(UM230123BE) 1/2

YMC USER'S MANUAL【Normal phase】
・ In general, n-hexane, dichloromethane, chloroform, and ethanol are recommended for regular use.
・ Addition of dichloromethane, chloroform, and alcohol like ethanol to n-hexane is basic. Acetonitrile, methanol, tetrahydrofuran (THF),
dioxane etc. are also usable.

・ In case that elution of basic compounds is difficult, acid like acetic acid and trifluoroacetic acid can be added to enhance solubility of
the compounds. Triethylamine etc. are also available for additives.
【For all products】
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ Aqueous or non-aqueous solvent can be used as a mobile phase. Repetitive replacement among solvents with large difference inpolarities might degrade the column performance.
・ When the displacement from aqueous to non-aqueous solvent or vice versa, flush the column with more than 10 column volume ofcompatible solvent like 2-propanol and THF.
・ Recommendations of pH and temperature for column use are shown in the specifications table in section 2. Column lifetime isstrongly dependent on pH, temperature, and mobile phase composition. In general, high temperature and concentration of buffer oradditive, low organic solvent concentration will shorten column lifetime.

6. Column cleaning (general method)【Reversed phase】
・ Flush the column with solution containing a higher ratio of organic solvent for washing out the compounds that have a great capacity
for retention in the column after using mobile phases not containing buffer salts/additives. Usable concentration of organic solvent isup to 100%.
・ When using mobile phase containing buffer salts/additives, first replace with a water/organic solution containing no buffersalts/additives (A ratio of water to organic solvent should be set at the same proportions as a mobile phase). Then flush the column inaccordance with the method described above. Mobile phase containing about 50 mM or less in buffer salts/additives can be replaced
directly with 60% acetonitrile aqueous solution.
・ Analyte and salts are likely to remain in CD BR or PREP CD columns, compared with ODS column. Wash the column sufficiently(flushing more than 10 column volume) after usage.
【Normal phase】
・ Flush the column with 2-propanol etc.

・ Replace with the mixture of n-hexane and alcohol etc. for storage.

7. Other environments
・ The operating pressure should be kept under 20 MPa (2900 psi), for 10 mm I.D. column or more under 10 MPa (1450psi).
・ To prevent exposure of the column to excessive pressure, the sample solution should be filtered through a 0.2 μm membrane orsmaller to remove particulates. We recommend using a pre-column filter to prevent the column frit from being clogged withsamples.
・ Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
・ Adjust the flow rate appropriately because the pressure changes depending on the column length, temperature, types of organic
solvent etc.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM230123BE) 2/2