Annex 5

FNCA MB Workshop Question & Answer Sheet
Reporter

Question / CommentAnswer / Response

Bangladesh

Dr. A.N.K. Mamun, I just would like to clarify if

Its land race grain is also black even after(Dr. Mamun)

the black rice that you collected recently is acooking.

pigmented rice. I presume that it is an uplandtraditional variety or landrace which is an
important

germplasm

material.

Iamwondering if the black color appears only on
the rice hull, but the grains are like your otheraccessions or the grains remain black evenafter cooking. (Mr. Aurigue, Philippine)
China1) I have a rice mutant line that I showed in1) Dear Dr. Hase, it will be an honor to work on(Prof. Shu)

my presentation, but because I have only one

your gene via genomic editing to reconstitute theallele, I am thinking of performing genome

mutant phenotype you observed. Please just let

editing to prove the responsible gene. Is itme know the gene, the mutation and thepossible to work together on this gene? I justphenotype.

want to check if another allele shows a similar

Thank you very much, Prof. Shu. It is very goodwithering phenotype. Thank you very muchof you to say so. I will write to you after making

for your consideration. (Dr. Hase, Japan)

sure the segregation ratio after backcrossing to2) Thank you very much for sharing with us

the wild type. (Dr. Hase, Japan)

your recent publication, Prof. Shu! Your report2) Dear Mr. Aurigue, the hybrid is a cross between

is very interesting, but I did not understandJiang 79S (japonica, an early flowering mutant)what you said about the hybrid rice,and DR610 (indica, a doubled haploid line).

especially the japonica/indica hybrids. Does
any of the parent of the hybrid, whether
japonica or indica, a product of mutation
breeding or possesses a mutated trait? (Mr.
Aurigue, Philippine)
Indonesia

I am wondering if you obtain other seed colors

Yes Mr. Fernando, we have brown soybeans(Dr. Puspitasari)(brown or red) of soybeans aside from blackmutantand white. Do the farmers or consumers likeyellow/white one.

black soybeans? I hope that seed quality

Black soybeans are needed mostly as raw

could also be evaluated to determine thematerial of soysauce in Indonesia. We use thevalue of mutant soybean varieties in tempe-

yellow one for tempe nowadays, but originallymaking, especially for the black mutant which

tempe was made from black soybeans.

is expected to have better nutritional value.

Thank you for your interest.

Thankyou,Dr.

Winda!

(Mr.

Aurigue,Philippine)

1from

gamma

irradiationoftheJapan

I appreciate your report, Dr. Hase!

You

Thank you very much for your comment, Mr.

(Dr. Hase)

spoke very clearly, and your discussion wasFernando. I do not know how it goes, but I am

easy to understand. It also shows thevery interested in the hyper-salt-tolerant mutant

importancederivedofmember states.

collaboration

withotherWe look forward to thedetermination of the mechanism for salt

fromthesalt

tolerant

variety

BRRIdhan47. I am glad if there is something we
can work together in future.

tolerance in mutant rice varieties if not due toa novel gene. (Mr. Aurigue, Philippine)
Korea

Thank you very much, Prof. Si-Yong Kang for

To Dear Mr. Aurigue. Thank you very much for(Prof. Kang)

sharing with us your two publications andyour interest and response to my presentationcomparison of the effects of proton beam andand hop research.

gamma rays! Your new research on mutation
breeding of hops is very interesting! (Mr.
Aurigue, Philippine)
MalaysiaYour report is so inspiring and gives us hope

Dear Mr Aurigue, thank you very much for your(Dr. Hussein)that we could succeed too. Thank you very

complimentmuch and congratulations, my Friend Sobri!

Philippine team did a very good job as well in(Mr. Aurigue, Philippine)

mutation breeding research. Congratulations toandkindwords.

Clearly,theyou too. Thank you to all FNCA members andCabinet Office of Japan for making this FNCA
project a fruitful one. It is my pleasure to be partof this excellent FNCA team.
Mongolia

The changes in seed color and spike type(Dr. Noov)observed at M2 would be most interesting toobserve in barley. I wish you great success,Dr. Noov, in developing a variety with newseed color or better nutritional value. (Mr.
Aurigue, Philippine)

The Philippines(Mr. Cabusora)

ThailandWe really hope that you will discover a newWe are now trying to prove that the mutant lines(Mr. Noenplab)

gene for submergence tolerance if it is notfor submergence tolerance carry the Sub1 allele

Sub1 gene. We wish you all the best! (Mr.

by comparing the gene sequence to non-mutant

Aurigue, Philippine)

varieties carrying the Sub1 allele. At the sametime,

wewilllookfor

QTLs

involving

submergence tolerance traits by QTL analysis of2

F2:3 populations from crosses between tolerantmutantlines

andnon-mutant

susceptible

genotypes. We hope to discover new QTLs forsubmergence tolerance in the mutant lines.
VietnamInstead of albino (white) variation in peanut, I

Dear Mr. Aurigue, many thanks for your comment.

(Dr. Le)

believe

chlorophyll

I also agree with the assessment that some are

mutation was xantha (yellow) variation. It

xantha, we will continue to evaluate thewould be interesting to note the chlorophyll

heritability of this trait in the next generation.

mutations in the succeeding generations. I

Regarding the red seed trait, which is a valuable

hope that you will also monitor the red seedtrait related to seed quality, we will continue tocoat color mutant, which might indicate higher

screen these lines along with the yield trait. We

anthocyanin level or enhanced nutritional

are hoping to be able to submit some newvalue, as well as the mutant lines with more

prospect lines for testing in 2022. Once again

seeds or larger pods that may contribute tothank you and best wishes to you and the FNCA

yield increase. Thank you very much, Dr. Le

members.

thattheobserved

Duc Thao! (Mr. Aurigue, Philippine)3