PROTEINLABELING KIT

VivoTag™ 680XL
Protein Labeling Kit

Labeling kit contents:• 2 X 0.25 mg of VivoTag 680XL• 1 X 1 mL of 1M solution of sodium bicarbonate (pH 8.3)

PerkinElmer’s VivoTag 680XL Protein labeling kit isdesigned for preparing fluorescently labeled antibodies,
proteins or peptides for small animal in vivo imagingapplications. Each kit contains our superior in vivo
optimized VivoTag 680XL fluorophore and everything
you need to carry out the reaction and purify thelabeled product.

• 2 X Purification column• 4 X 15 mL conical collection tube
• 1 X PBS (50 mL)

VivoTag dyes are used in PerkinElmer's in vivo fluorescenceagents and have been optimized and validated for in vivo
imaging on PerkinElmer's comprehensive array of imaging
platforms. Label your proteins, antibodies or peptides withthe fluorescent dye that in part made PerkinElmer'sfluorescent agents the best-in-class for in vivo imaging.
VivoTag 680XL labeling kit offers key benefits for
effective in vivo labeling of your protein.
• Label any protein, peptide or antibody. One kit labelsup to 10 mg of protein offering greater flexibility inyour model.
• VivoTag dyes are bright and extremely photostable forextended periods, providing more efficient labeling, andare designed and optimized for in vivo use.
• Use the dyes that have been validated and optimizedon all of PerkinElmer’s world renowned in vivo
imaging systems.

Absorbance and fluorescence emission spectra in 1 x PBS.

Physical and Spectral Properties
PROPERTY

SPECIFICATION

MW of VivoTag 680XL

1856 g mol-1

Fluorescence Emission max

688 nmAbsorbance max

668 nmExtinction210,000 M-1 cm-1

Purity>95 %AppearanceBlue solid

IgG

IgG

Ab Conjugate

Ab Conjugate

IgG

Ab Conjugate

Tumor targeting of antibody conjugated with VivoTag 680XL utilizing the protein labeling kit. Nu/nu mice were injected with MDA-MB-231-luc2 orthotopically. Bioluminescenceimaging (BLI) on the top shows similar tumor growth in both mice. 10 μg of antibody specific to these tumors (n=3) and IgG (negative control) were conjugated with VivoTag 680XL.
VivoTag antibody conjugates were injected intravenously and imaged at various time points using the IVIS Spectrum and FMT instrument. Fluorescence imaging (FLI) shows specifictargeting to the tumor while no signal from the tumor is observed from IgG conjugated to VivoTag 680XL. The chart shows fluorescence signal expressed in total radiation efficiency
from the tumor with IgG and tumor specific antibody. Bottom panel shows FMT images in reflectance (left) and tomography (right) mode. The high liver signal is due to thedistribution kinetics of the antibody. Images shown are at 24 hours post injection of the antibody conjugate.

IVIS® Spectrum CTFMT® 4000

Maestro™ 2

Quantum FX μCT

VivoTag dyes are optimized for use on all PerkinElmer in vivo imaging systems.
PerkinElmer in vivo imaging reagents are intended for animal research and not for use in humans.

Learn more at www.perkinelmer.com/invivoreagents

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Copyright ©2012, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.
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Printed in USA

August 2012