Annex 3. Session Summary

Session Summary ofFNCA 2017 Workshop on Mutation Breeding Project

Session 1Follow-up on Sub-project on Sorghum & Soybean, and Banana

Follow-up reports on the Sub-projects in Sorghum & Soybean and Banana were presented.
The summaries are as follows:
1. Sub-project on Drought Tolerance in Sorghum and Soybean(Dr. Sobrizal, BATAN, Indonesia)
So far, 3 sorghum and 10 soybean varieties have been released by BATAN, and they havegrown largely with total growing area in 2016 for Sorghum 1.600 Ha, for soybean 600 Ha.
Recently, soybean variety Mutiara 1 has been selected and used as the basis for national
self-sufficiency plan, which aims to increase food security in the country. To increase thedomestic production in coming years Mutiara 1 seeds are being mass-produced anddistributed to farmers. In addition, some sorghum mutant lines with high grain yield andbiomass production, and some soybeans mutant lines with drought tolerance and earlymaturity have been developed and will be submitted to national variety release committee
for evaluation.
2. Sub-project on Disease Resistance in Banana(Ms. Ana Maria S. Veluz, PNRI, the Philippines)
The project’s main objective aims to make “Lakatan” banana production more profitable tosmall farmers by reducing losses due to Banana Bunchy Top Virus (BBTV) infection by
20% through adoption of disease resistant cultivars. This was successfully attained as partof the integrated management strategies against BBTV through the development of BBTV
resistant Lakatan by gamma-irradiation. Based on several disease reaction parameters
used5

mutantlines

namelylines

9-28-2(SAGANA1),

9-28-3(SAGANA2),

13-30-2(SAGANA 3), 22-28-2(SAGANA 4) and 28-30-2(SAGANA 5) were selected andevaluated in multi-locations trial like Luzon, Visayan and Mindanao. These 5 mutant lines
called HIBRID LAKATAN or Highly Improved Banana through Radiation Induced
Technology were recommended for registration as new Lakatan variety with 5 lines to theNational Seed Industry Council of the Philippines (NSIC) and waiting for the release of its
recommendation anytime this year.

Session 2

Country Report on Application of Mutation Breeding of Rice for Sustainable
Agriculture

Nine member countries presented current progress and activity summary for 5 years on theapplication of mutation breeding of rice for sustainable agriculture. The brief summaries are-1-as follows:
Bangladesh (Dr. A.N.K. Mamun, BAEC)BINA Dhan -14 is becoming more and more popular to farmer in the different area of
Bangladesh and cultivation area is also increasing. Mutant line is irradiated with carbon
ion beam and selected as a mutant line from BRRI dhan 29 and named as BINA Dhan -18.
This high yielding early mature line is released as new mutant variety in 2016. Recently
another variety called BINA Dhan-19 is also released this year of 2017 from the parent
variety NARICA-10. This mutant variety is selected from the seeds of NERICA-10 varietyof rice and irradiated with 40 Gy carbon ion beams from QST Takashaki in 2013. This newvariety can be cultivated in rain-fed condition in both AUS and Aman season inBangladesh.
China (Dr. Huang Jianzhong, ZU)
By applying gamma-irradiation and related biotechnologies: we obtained ten mutants
including three early maturing mutants, two putative blast resistant mutants, two low
phytic acid mutants and one xantha revertant. An HRM-based TILLING system suitablefor fast and high-throughput screening of indel mutations was established by combining
HRM analysis and gamma mutagenesis. We identified three mutated genes and further
elucidated the functions and underlying mechanisms in the mutants: one epigenetic
mutation of the OsGUN4 and its reversion mutant OsGluTR, one lesion mimic mutation ofCYP81A1.
Indonesia (Dr. Sobrizal, BATAN)
Twelve promising mutant lines with high yields and early maturities have been developed
through a wide cross and mutation breeding program. The growth durations of thesemutant lines were ranging from 93.7 to 99.3 days from sowing to harvesting, significantly
shorter than those of original line SKI 88, national leading variety Ciherang and the earlymaturity national leading variety INPARI 13. Yield of most mutant lines were notsignificantly difference with that of original line, SKI 88 and national leading variety,Ciherang, but significantly higher than that of INPARI 13. To fulfill the requirement ofvariety release in Indonesia, other multi-location yield trials and other examinations suchas pests, diseases as well as other grain quality examinations should be conducted.
Japan (Prof. Hirokazu Nakai, Shizuoka University)
Breeding for adaptability to low input sustainable agriculture using mutant lines with highresistance to bacterial leaf blight has been continued for 13 years. In 2017, it wasconfirmed that some selected lines had significantly higher grain yield and better eating
quality as well as adaptability to the conditions of tropical Asian countries. In the crossbreeding in several place across in Japan, some mutant lines showed higher yield combinedwith good eating quality. It is expected that they will be irradiated by gamma-ray and ion
beams to remove unfavorable characters.
-2-Korea (Dr. Kang Si-Yong, KAERI)
A new mutant rice variety with salt tolerant, Wonhae-2, was derived from an in vitro
mutagenized population of Dongan induced by 70 Gy gamma ray irradiation. The mutant
with elevated salt tolerance was selected to salt stress through seedling, pot and reclaimed
soil test at various growth stage of M2-M4 generation. Tocomi-1’ and ‘Tocohongmi’, newjaponica rice cultivars derived from a 300 Gy gamma ray irradiation with high tocopherol
content and red- brown pericarp. Among them, ‘Tocomi-1’ was officially registered by theKorea Seed and Variety Service after 2-years field evaluation test and then transferred aprivate company, “Seedpia” for commercialization.
….
Malaysia (Dr. Sobri Bin Hussein, Nuclear Malaysia)
FNCA Mutation breeding project has been selected as one of the high impact projects withlow cost involved. The first meeting with secretary general (MOSTI) was held on 20 Feb
2017 and followed by technical meeting with Deputy Director General MARDI on 15 March2017. In the meantime, NMR 152 (PVBT 027/15) sample has been submitted to Malaysian
Agriculture Research and Development Institute MARDI on 18 Sep 2017 to initiate thelegalization/certification process.
The Philippine (Ms. Ana Maria S. Veluz, PNRI)This project aims to develop mutants with improved agronomic characteristics by gamma
irradiation that can adapt to organic farming. Gamma irradiation significantly reduced thenumber of days to flowering and increased the number of tillers/plant in both theirradiated plants (200Gy and 300Gy) of the a traditional rice varieties, Umangan andNative Borie, at M3 generation while reduction in plant height at maturity for Native Boriein both irradiated ones (200Gy and 300Gy) was observed. The addition of carrageenan asplant growth promoter for the 2 rice varieties and soaking in biofertilizer enhanced theflowering and production of tillers when applied at the right time and concentration.
Selected potential mutant lines from M3 generation are being grown as M4 generation andfurther evaluation and selection of promising mutant lines responsive to organic farming
will be undertaken for the M4 generation.
Thailand (Dr. Kanchana Klakhaeng, RD)
Three induced mutant rice varieties in Thailand are RD6, RD15 and RD10 which were still
well accepted by farmers and consumers worldwide. Promising lines of mutants rice wereidentified as submergence tolerance and anaerobic germination ability. More informationis needed from M5 plants for further conclusions.
Vietnam (Dr. Le Huy Ham, AGI)
During FNCA forum 2007-2017 have been released 10 rice mutant varieties. Some of them
became very popular variety like Khang Dan mutant, DT80. By using ion beam for-3-irradiation have been derived number of promising lines which are now are being tested in
the filed conditions. Combination of mutation and molecular marker for rice breeding
become routin in rice breeding, thus shorten time and improve selection efficiency.

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