109

Center for Experimental Medicine and Systems Biology

Core Laboratory for Developing Advanced Animal Models
先進モデル動物作製コア
Professor
Visiting Professor
Associate Professor
Project Assistant Professor

Tomoji Mashimo, Ph.D.
Kimi Araki, Ph.D.
Manabu Ozawa, Ph.D.
Jumpei Taguchi, Ph.D.

教 授
客員教授
准教授
特任助教

博士（人間・環境学） 真博士（理学）荒博士（農学）
小博士（医科学）
田下 知 士
木 喜 美
沢学
口 純 平

The Core Laboratory for Developing Advanced Animal Models supports basic
sciences in the life science field by producing and providing gene-manipulated miceor rats such as human disease models or gene KO/KI models. Using cutting-edge
genome editing techniques, we make various types of gene-manipulated animals,
including indel mutation, large fragment deletion, SNPs, conditional Cre/loxP, drug
inducible gene expression/silencing, reporter gene KI, or gene conversion formaking humanized mice or rat models either by direct gene editing in zygote or
highly efficient ES cell-mediated gene targeting followed by chimera animal
productions.
https://www.ims.u-tokyo.ac.jp/cemsb/public_html/index.html

Laboratories that consist of the Core
‘Core Laboratory for Developing Advanced Animal Models’ was launched in 2020 to provide
gene-manipulated mouse or rat models to domestic
or international academic institutions. One division,
the Division of Genome Engineering, and two laboratories, the Laboratory of Reproductive Systems Biology and the Laboratory of Genetically Engineered
Mouse Research, all of which belong to the Center for
Experimental Medicine and Systems Biology, comprise the Core.
Cutting-edge genome editing techniques
For making indel mutants, large deletion, or short
DNA fragment KI such as SNPs, or peptide tags, weoffer direct genome editing using mouse or rat zygotes through NEPA electroporation systems (NEPAGene). In mice, embryos from C57BL/6J strain areroutinely served for genome editing, but other strains,such as C57BL/6N or BDF1, are also applicable if necessary. In the rat, F344/Jcl strain is served for zygote

genome editing. For large-size gene manipulations inmice, such as Cre/loxP conditional allele, fluorescein
reporters KI, gene conversion from mice to human, ordrug-inducible Tet-on/off system, we offer CRISPR/Cas9-assisted plasmid KI using ES cells through Neon
Electroporation system (ThermoFisher) followed byblastocyst injection for developing chimeric mice. EScells from C57BL/6J, C57BL/6N, 129, B6129F1, orBALB/c strains are available for chimera productions.
For producing large-size gene-manipulated rats, e.g.,reporter KI or humanized rat models, the direct zygote genome editing technique, termed Combi-CRISPR, is applicable.
Supporting gene-manipulated mouse or rat model
production through the core lab and AdAMS platform
We provide cutting-edge animal production techniques through our core lab and Advanced Animal
Model Support, AdAMS. Our core is a member of AdAMS, which belongs to the Committee on Promoting
Collaboration in Life Science, MEXT, and is an aca-110demic platform for producing gene-manipulated animals. Therefore, researchers earning KAKENHI,
Grant-in-Aid for Scientific Research, can apply to thisplatform.
Number of mice or rat strains we developed in2023

In 2023, our core provided 13 or 11 strains ofgene-manipulated mice through the core lab or AdAMS, respectively. In the rat case, 4 strains ofgene-manipulated rats have also been provided
through AdAMS.