05

Hybrid Silica Based Columns

YMC-Triart -------------------------------------------- 42-47
YMC-Triart [Metal free column] -------------------------- 48
YMC-Triart C18---------------------------------------- 49-51
YMC-Triart C18 ExRS------------------------------------- 52
YMC-Triart C8 -------------------------------------------- 53
YMC-Triart Phenyl --------------------------------------- 54
YMC-Triart PFP ------------------------------------------ 55
YMC-Triart Bio C4 ---------------------------------------- 56
YMC-Triart Diol-HILIC------------------------------------ 57
Ordering Information---------------------------------- 58-60

05

Hybrid Silica Based Columns

YMC-Triart
YMC-Triart is organic/inorganic hybrid silica based column, emphasizing versatility. The main features are superior durability, peak
shape across all kind of compounds and reproducibility.
Having the same selectivity across different particle sizes, smooth method transfer between UHPLC and HPLC can be performed.
Moreover, various bonded phases supplement performance of C18 phase, and allow separations which C18 columns cannot achieve.

Various product lineup enables wide range of separation from UHPLC to HPLC analysis andeven to preparative separation.
Features

Hybrid Silica Based Columns

l Effective for method screening with various chemistries
l Great chemical durability provided by hybrid particles
l Superior peak shapes for a wide range of compounds and in various conditions
l Smooth method transfer from UHPLC to HPLC analysis and even to HPLC purificationl Available in metal free column for separation of coordination compounds

Versatile hybrid base material
YMC-Triart is based on novel organic/inorganic hybrid particles. The particle combines high mechanicalstability and high efficiency derived from silica based packing material and high chemical stability derived
from polymer based packing material. The granulation process utilizing microreactor technology enables
continuous and highly controlled production of hybrid particles. The particle has uniform pore size
distribution and smooth surface as well as uniform particle size. This feature greatly contributes to excellent
peak shape and separation reproducibility.

Specifications
Bonded phase

Triart C18

Triart C18 ExRS

Separation
mode

Particle size(μm)

-C18H37(standard type)

Pore size
(Å)

C% *

120

20

Endcapping

UsablepH range

USP
Classification

L1-C18H3780(high density bonding)

25

1-12
Yes

Triart C8-C8H17

Triart Phenyl-(CH2)4

Triart PFP-(CH2)3-17

1.9, 3, 5

F

F

F

F

-C4H9

Triart Diol-HILIC-CH 2 CHCH 2
OH OH-

Triart Bio C4

-

120

F

*Containing carbon content of hybrid silica base material.

42

L7

Reversedphase

HILIC

17

1-10

L11

15

No

1-8

L43

300

-

Yes

1-10

L26

120

12

No

2-10

L20

Excellent durability【Durability in high pH】
Triethylamine (pH 11.5), 40℃

120%% of initial theoretical plate number% of initial theoretical plate number

Phosphate buffer (pH 11.5), 40℃
Triart C18 ExRS

100%

Triart C18

80%

Triart C860%

Hybrid based C18

40%

Silicabased C18

20%
0%

0

20

40

60

Time (hr)

80

120%

Triart C18
Triart C8

100%
80%
60%
40%

Silicabased C18

20%
0%

0

100

100

150

200

Time (hr)

250

300

Column: 5 μm, 150 X 4.6 mmI.D.
Eluent: 50 mM triethylamine (pH 11.5)/methanol (90/10)
Flow rate: 1.0 mL/min
Temperature : 40℃
Sample: benzyl alcohol

Hybrid Silica Based Columns

Column: 5 μm, 150 X 4.6 mmI.D.
Eluent: 50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)
Flow rate: 1.0 mL/min
Temperature : 40℃
Sample: benzyl alcohol50【Durability in high temperature】
pH 1, 70℃
120%

120%
100%
80%

Silica based C18(alkaline resistant type)

60%
40%

Silicabased C18

20%
0%Triart C18
Triart C18 ExRS

100%

Triart C18% of initial retention% of initial theoretical plate number

pH 6.9, 70℃

Silicabased C1880%

Hybridbased C18

60%
40%
20%

0

100

200

300

400

500

0%

600

Time (hr)Column: 5 μm, 50 X 2.0 mmI.D.
Eluent: 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (90/10)
Flow rate: 0.2 mL/min
Temperature : 70℃
Sample: phenolThe columns are kept in acetonitrile/water/TFA (10/90/1, pH 1)
at 70ºC, and tested for performance every 20 hours

0

20

40

Time (hr)

60

80

100

Test conditions Column: 5 μm, 50 X 2.0 mmI.D.
Eluent: acetonitrile/water (60/40)
Flow rate: 0.2 mL/min
Temperature : 37℃
Sample: butyl benzoate

With innovative surface modification on organic hybrid silica, Triart columns show great chemical durability and they can be used over a wide pH range. Even at
high-pH or high-temperature conditions, the lifetime of Triart C18, C18 ExRS and C8 is more than 10 times greater than that of conventional C18 columns anda few times greater than commercially available high alkaline-resistant C18 columns. When using under alkaline condition, organic buffers such as triethylamine
make the column life longer than phosphate buffer. In addition, Triart is ideally suited for preparative purifications of various compounds or peptide analysis in
the cases where trifluoroacetic acid (TFA) is frequently used, because it has high resistance to acids.

【Long column lifetime under chemically harsh conditions】
1

Barbiturates
1. Barbital
2. Phenobarbital
3. Hexobarbital
4. Pentobarbital
5. Secobarbital

2
3 4 51000th
800th
600th
400th
200th
1st
0

1

2

3

4

5

6

7

min

% of initial retention(%)

Continuous analysis with alkaline mobile phase (pH 9.5)

Secobarbital

100
80
60
40
20
0

0

200

400

600

Run number

800

1000

ColumnEluent: YMC-Triart C18 5 μm, 50 X 2.0 mmI.D.
: A) 20 mM HCOONH4-NH3 (pH 9.5)
B) methanol
0-90%B (0-7 min)Flow rate: 0.2 mL/min
Temperature : 25℃
Detection: UV at 240 nm
Injection: 1 μL
Triart shows great durability under alkaline mobile phase conditions, which
is difficult for conventional silica columns. This assures stable analysis
over a long period of time.

43

05

Hybrid Silica Based Columns
Great peak shapes without adsorption/peak tailing【Comparison of chromatographic behavior】
Acidic compounds

Basic compounds
H3C

O

Dextromethorphan
N

OH

1 2

3

Triart C18

Triart C8
1

Hybrid based C18
Brand I8

H3C

3

2

1

2

V110706G

2

O

3

1

Q090805B

3

2

V110704B

1

V110713C

3

2

3

Hinokitiol（Peak 1）HO

1
V110704G

2

2

CH3

（Peak 1）

O

CH3

1

Salicylic acidOH

（Peak 2）

Coordination compounds

2

V110705E2

1
1

Hybrid Silica Based Columns

Q090123F

1 2

Hybrid based C18
Brand G4

3

Q090806B

3

2

3

Silica based C18
Brand A2

Silica based C18
Brand C3
1

Silica based C18
Brand D5

1

2

Q090805C

1

3

3

1

2.5

5.0

7.5

10.0

12.5

15.0

Q081219C

2

1

Q090821C

02

Q090807B

3

2

Q090406C

1

3

2

Q090205E

2

Q081226A

2

1

Q090408F

1

Q090120A

2

1

1
Q080205F

2

1

Q090807C

3

2

Q090910E

17.5 minColumn: 5 μm, 150 X 3.0 mmI.D.
or 150 X 4.6 mmI.D.
Eluent: 20 mM KH2PO4-K2HPO4(pH 6.9)/acetonitrile (65/35)
Flow rate: 0.425 mL/min for 3.0 mmI.D.,
1.0 mL/min for 4.6 mmI.D.
Temperature : 40℃
Detection: UV at 235 nm
Sample: 1. Chlorpheniramine
2. Dextromethorphan
3. Propyl paraben (I.S.)

0

2.5

5.0

7.5

10.0

12.5

Q090820D

15.0 min0

Column: 5 μm, 150 X 3.0 mmI.D.
or 150 X 4.6 mmI.D.
Eluent: 10 mM CH3COOH-CH3COONH4(pH 4.2)/acetonitrile (75/25)
Flow rate: 0.425 mL/min for 3.0 mmI.D.,
1.0 mL/min for 4.6 mmI.D.
Temperature : 40℃
Detection: UV at 254 nm
Sample: 1. Salicylic acid
2. Methyl paraben (I.S.)
3. Cinnamic acid

2.0

4.0

6.0

8.0

10.0

12.0 minColumn: 5 μm, 150 X 3.0 mmI.D.
or 150 X 4.6 mmI.D.
Eluent: acetonitrile/0.1% H3PO4(40/60)Flow rate: 0.425 mL/min for 3.0 mmI.D.,
1.0 mL/min for 4.6 mmI.D.
Temperature : 40℃
Detection: UV at 254 nm
Sample: 1. Hinokitiol
2. Methyl benzoate (I.S.)

Superior peak shapes across various mobile phases【Peak shape comparison of basic compound】
10 mM phosphate buffer (pH 6.7)/organic solvent
Methanol
Triart C18

Silica based C18
Brand D5
Silica based C18
Brand I6

Tf 1.03

Tf 1.16

Tf 1.14

Tf 1.88

Tf 1.22

Tf 1.78

Tf 1.21

Tf 1.56

Tf 1.13

Tf 1.43

F090727H07

F090727I07

F090727F07

F090803G07

Tf 0.93

Tf 2.83

Tf 1.05

Tf 2.21F090717H07

F090717I07

F090714F07

F090714G07

F090818I07

H 3C
N

O

Cl

44

min

Tf 1.13

F090722I07

6.0

7.0

8.0

min

F090731G07

Tf 1.88

F090722F07

8.0

9.0

10.0

: 5 μm, 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D.
: A) 10 mM KH2PO4-K2HPO4 (pH 6.7) or 10 mM CH3COONH4 (pH 6.7)
B) methanol or acetonitrile
5-90%B (0-10 min), 90%B (10-15 min)Flow rate: 0.2 mL/min
Temperature : 25℃
Detection: UV at 230 nm

minF090722G07

6.0

7.0

8.0

min

ColumnEluentCH3

Clemastine

12.0

F090817G07

F090731F07

Tf 3.87

F090722H07

11.0

F090818F07

F090731I07

Tf 1.03
10.0

Acetonitrile

Tf 1.11

F090731H07

Silica based C18
Brand C1

MethanolTf 0.97
F090818H07

Hybrid based C18
Brand I8

10 mM CH3COONH4 (pH 6.7)/organic solvent

Acetonitrile

Clemastine is a well known basic compound
which can easily tail on conventional ODS
columns. Triart C18 can analyze clemastine
without any peak deterioration with any
kinds of buffer/solvent combinations.

Comparison of separation selectivity among YMC-TriartmAU
120

π-π interaction index

80

Triart C18

2

1

40

Hydrophobicity indexα(4/7) 0.42

3ｋ’(7) 9.95

654

Shape recognition ability index
α(9/8) 1.21

7

8

0
0

5

10

15

9

20

25

P120830N

minmAU

α(4/7) 0.48

120

1

40

2 3

4,5

6

7

8

9

0
0

Triart C8 shows similar selectivity to
Triart C18, but shorter retention times
than C18.

α(9/8) 1.13

5

P120828D

10

15

min

ｋ’(7) 3.37α(9/8) 0.98

mAU
120

α(4/7) 0.58

80

Triart Phenyl

2

40
0

3

1

54

0

6

7

On Triart Phenyl and Triart PFP, π-π interaction
and polar interaction as well as hydrophobic
interaction contribute to separation. Theyshow different separation selectivity from
Triart C18 or Triart C8.

9,8P121206A

5

10

min

mAU

1. Amitriptyline (basic compound)
2. 8-Quinolinol (coordination compound)
3. Testosterone (polar compound)
4. Naphthalene (π-π interaction)
5. Ibuprofen (acidic compound)
6. Propylbenzene (hydrophobicity)
7. n-Butylbenzene (hydrophobicity)
8. o-Terphenyl (shape recognition ability)
9. Triphenylene (shape recognition ability)120α(4/7) 0.90

80

Triart PFP

2

40

5

3,4
6 1,7

ｋ’(7) 2.23α(9/8) 3.06

8

9

0
0

5

10

9.95

Triart C8

5.53

Triart Phenyl

0.24

Triart C8

0.26

5

10

15

20

0.90

0.0

25

Triart C8

Triart Phenyl

Triart PFP

3.06

0.5

1.5

2.5

3.5

1.5

A mixture that consists of compounds withvarious characteristics is analyzed with reversedphase Triart columns. In addition to hydrophobic
interaction, secondary interactions such as π-πinteraction and polar interaction are different
from column to column. Those parameters
have great impact on retention capacity (k’) andseparation factor (α). By utilizing the difference
in separation characteristics, wide range ofcompounds can be well-separated with Triart.

0.48

Triart Phenyl

0.98

1.0

0.42

Triart C8

1.13

0.5

π-π interaction α(4/7)
Triart C18

1.21: 5 μm, 150 X 3.0 mmI.D.
: 20 mM H3PO4-KH2PO4 (pH 3.1)/
methanol (25/75)
Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 265 nm
Injection: 4 μL

0.44

Shape recognition ability α(9/8)
Triart C18

ColumnEluentTriart PFP

2.23

0

Triart C18

Triart Phenyl

3.37

Triart PFP

P121206B

15 minPolarity α(3/7)

Hydrophobicity k’(7)Triart C18

Hybrid Silica Based Columnsｋ’(7) 5.53

80

Triart C8

0.58

Triart PFP

0.90

0.0

0.5

1.0

1.5

45

05

Hybrid Silica Based Columns
Ideal for UHPLC analysis【Correlation between linear velocity and column efficiency】
Van Deemter curves
0.030

Triart C18 50 X 2.0 mmI.D.

HETP (mm/plates)

0.025

5 µm

0.020

0.015

3 µm

0.010

1.9 µm

0.005

0.000

Eluent: acetonitrile/water (60/40)
Temperature : 25℃
Sample: butyl benzoate

0

2

4

6

8

10

Triart 1.9 µm columns exhibit higher efficiency and maintain efficiency overa wide range of flow rate compared to 5 µm and 3 µm columns.

12

Hybrid Silica Based Columns

Interstitial linear velocity (mm/sec)

X axis : Interstitial linear velocity (Obtained by dividing column length by dead time (t0); the larger number means faster flow rate.)
Y axis : height equivalent of a theoretical plate (HETP; Obtained by dividing theoretical plate number by column length; the smaller number means higher column efficiency.)【Method transfer between HPLC and UHPLC】
Agrichemicals

mAU

HPLC method

1

20

1.

5%B (0-3 min)
5-30%B (3-10 min)30-45%B (10-55 min)
0.2 mL/min

Triart C18
150 X 2.0 mmI.D. 5 µm

4.5 MPa(650 psi)

15

7
2

3
＊

0

4

5

O

H3C

20

50

40

30

UHPLC method
mAU

Triart C18
50 X 2.0 mmI.D.

1

NH

55

20

3.

N

S

Analysis time
1/9

7
15

8 9

4

0

1

2

3

4

6

5

0
0

6

10

20

30

: A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
Temperature : 40℃
Detection: UV at 240 nm
Injection: 1 μL (5 μg/mL)

4050＊Impurity derived from thiram

min

NH
O

Siduron

8.
O
OH

H3C

H3C

O

N

NH
N

N

Triclopyr

J110121B

0

NH

O

Cl

5
5

CH3

CH3

4.

Cl

6

＊

7.
N

Thiram

Cl

3

2
10

S

O

5

N
O

CH3

CH3

10

S

O

O

Flazasulfuron

S

CH3

H3C

F
NH

NH

O

S

15

N
N

Asulam39 MPa(5660 psi)

20

mAU

O

O

H3C

S

F

F

O

min

5%B (0-0.3 min)
5-30%B (0.3-1.0 min)30-45%B (1.0-6.0 min)
0.6 mL/min

1.9 µmMecoprop

6.

O

10

O
CH3

Cl

NH2

U101229A

0

O

2.

8 9

6

OH
O

N

Oxine-copper(Copper 8-quinolinolate)

10

5

Cu

N

5.
CH3

O

NH
O

O

N

N

Cl

S
O

O

O

CH3

CH3

Halosulfuron-methyl

9.
min

N

N
O

O
CN

H3C

O

O

CH3

O

Azoxystrobin

Eluent46

A 90% decrease of analysis time is achieved by transferring analysis method fromconventional HPLC using 5 µm particle to UHPLC using 1.9 µm particle at three times
faster linear velocity. Also, a method developed with UHPLC can easily be transferredto HPLC.

Quality control【Excellent reproducibility】
Packing material
Triart C18 exhibits excellent batch-to-batch reproducibility for all types of compounds including basic and coordination compounds
that often exhibits peak tailing or adsorption onto packing material.
Basic compounds

Coordination compound
1

2.5

5.0

J091201Y

1

3

7.5

10.0

1. Chlorpheniramine
3. Propyl paraben（I.S.）

12.5

15.0

17.5

2

Batch E

Batch E

Batch D

Batch D

Batch C

Batch C

Batch B

Batch B

Batch A

0.0

min

2.0

4.0

J091201X

6.0

8.0

10.0

12.0

Batch A

14.0 min1. Hinokitiol
2. Methyl benzoate（I.S.）

2. Dextromethorphan

Column: YMC-Triart C18 5 μm, 150 X 3.0 mmI.D.
Eluent: 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 235 nm

Column: YMC-Triart C18 5 μm, 150 X 3.0 mmI.D.
Eluent: acetonitrile/0.1% H3PO4 (40/60)
Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 254 nm

Packed column

Hybrid Silica Based Columns

0.0

2

20000

2.5

Theoretical plate number
15000

2.0

10000

1.5

Tailing factor
5000

Tailing factor

Theoretical plate number

Rigorous control of theoretical plate number (N) and tailing factor (Tf) is performed on Triart C18 packed column.

1.0

0

10

20

30

40

Column number

50

0.5

Column: YMC-Triart C18 5 μm
150 X 4.6 mmI.D.
Eluent: acetonitrile/water (60/40)
Flow rate: 1.0 mL/min
Temperature : ambient
Sample: butyl benzoate

Effective for high-sensitive analysis using LC/MS【Low bleeding】
LC/MS

Corona* CAD*
Scan ES+
TIC

60－ Triart C18－ No column

4.0E+08－ Triart C18－ C18 column A－ C18 column B

40pA

8.0E+0820

00.0E+00

ColumnEluent

0

2

4

6

8

10

0

12 min: 5 μm, 50 X 2.0 mmI.D.
: A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-1 min), 5-100%B (1-5 min), 100%B (5-10 min),100-5%B (10-10.1 min), 5%B (10.1-12.5 min)Flow rate: 0.4 mL/min
Temperature : 40℃
Detection: ESI positive, TIC (Mass Range: 50-1000)

6

12

18

24

30 minColumnEluent: 5 μm, 250 X 4.6 mmI.D.
: A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-5 min), 5-100%B (5-20 min), 100%B (20-30 min)Flow rate: 1.0 mL/min
Temperature : 40℃
Detection: Corona* CAD*On Triart column, very low level of bleeding (leaching) is achieved thanks to the improvement of production procedure and of durability. Background noise of
Triart C18 on LC/MS (TIC) is almost the same as blank run with no column. Also, baseline is almost stable on Corona CAD (Charged Aerosol Detector). These
results prove that there is little bleeding from Triart C18 column. Very low background noise and high S/N ratio even with high-sensitive detectors are expectedon Triart columns.
*Corona and CAD are registered trademarks of Thermo Fisher Scientific.

47

05

Hybrid Silica Based Columns

YMC-Triart [Metal free column]
l YMC-Triart materials packed in a “metal free” column hardware that consists of PEEK-lined stainless steel tube and PEEK fritl Suitable for analysis of coordination compoundsl High pressure tolerance guaranteed

Ideal for high sensitive analysis
YMC-Triart [Metal free column] consists by a double-layered structure: PEEK-lined inner surface and stainless steel outer tube. YMCTriart [Metal free column] is suitable for analysis of coordination compounds.

Hybrid Silica Based Columns

Packing material

PEEK

Wetted part (liner) : PEEK
Outer tube
: Stainless steel
Frit: PEEK

Stainless steel

Improved sensitivity on coordination compounds analysis
Metal free column

Standard column

mAU

mAU

8

3

8

2

6

6

1

4

4

2

2

0
0

1

2

3

4

NH2

1.
N
HO

P
OH

P
OH

O

O

P
OH

O

OH

N

min

1

N

ATP

: YMC-Triart C18 (3 µm, 120 Å)
50 X 2.1 mmI.D.
Eluent: 5 mM HCOONH4
Flow rate: 0.21 mL/min
Temperature : 25℃
Detection: UV at 265 nm
Injection: 1.0 µL (10 µg/mL)

1

NH2
N

HO

P

O

P

O

O

OH

OH

ADP

N

2

3

N

min

NH2
N

N

O
HO

P
OH

OH

4

3.

N

O

O

OH
OH

F161226C

0

2.

N

O

O
O

2

0

F161226A

O

3

O

O

OH

N

N

OH

AMP

Column48

Metal coordination compounds which have phosphate group in their structure tend to showpoor peak shape by interacting with metal, such as stainless steel column. On nucleotides
analysis shown above, better peak shape was obtained with the metal free column.
The metal free column is effective for highly-sensitive analysis using LC/MS, as well.

YMC-Triart C18
l Superior peak shapel Usable over wide range of pH and temperaturel Usable with 100% aqueous mobile phase

Pore size : 120 Å
Carbon content : 20%■ Usable pH range : 1-12
■ USP L1■■

Highly durable column suitable as a first choice
Main features of YMC-Triart C18 are great chemical durability and outstanding peak shape. YMC-Triart C18 can be used underconditions of wide range of pH or high temperature. Preferable balance of surface hydrophobicity and hydrogen bonding capacity areachieved by the optimization of density of C18 bonded phase. This feature enables YMC-Triart C18 a first-choice column suitable forvarious separations. YMC-Triart C18 also performs well with 100% aqueous mobile phase and superior retention and reproducibility
can be obtained.

Hybrid Silica Based Columns

Flexibility in method d evelopment【Efficient mobile phase screening for ionic compounds】
10 mM formate aqueous solution/acetonitrile

10 mM formate aqueous solution/
methanol1
2

pH 2.9

3

1

4 5

2

3

4

5

F090819B

F090819A

F090819D

F090819C

pH 6.1

pH 9.9F090820C

F090820D

0

1.

2.

O

S
O

H3 C

2

4

6

Saccharin（Acidic compound）
pKa=2.2

10

0

min

2

N
CH3

Dextromethorphan（Basic compound）
pKa=8.3: YMC-Triart C18 5 μm
50 X 2.0 mmI.D.
Eluent: A) 10 mM HCOOH for pH 2.9
10 mM HCOONH4 for pH 6.1
10 mM HCOONH4-NH3 for pH 9.9B) organic solvent
5-90%B (0-10 min), 90%B (10-15 min)Flow rate: 0.2 mL/min
Temperature : 25℃
Detection: UV at 230 nm

68

Amitriptyline（Basic compound）
pKa=9.4

10

12

CH3

min

5.
O

CH3
N

4

4.

3.

O

NH
O

8

CH3
O

HO

n-Butylparaben（Weakly acidic compound）
pKa=8.3

CH3

HO

CH3
O

CH3

Ibuprofen（Acidic compound）
pKa=4.4

ColumnOn reversed-phase HPLC, pH and organic solvent are the most important factors to control
retention and selectivity. Triart C18 with wide usable pH range offers significant advantage inselection of mobile phase condition. Triart C18 delivers symmetrical peak shapes for all typesof compounds. Moreover, this feature is independent from mobile phase pH and mobile phasecondition. Chromatographers can choose the most optimal condition by combining various mobile
phase conditions such as mobile phase pH, and types of organic solvent/buffer system.

49

05

Hybrid Silica Based Columns
Effective for an analysis of highly polar compounds using 100% aqueous condition【Retention stability under 100% aqueous mobile phase】～Image of C18 surface～
Nucleic bases

1.

NH2

2.

N
O

3.

O
HN
O

N
H

Cytosine

4.

O

HN
H2N

N
H

O

GuanineUracil

CH3

HN

NH

N

5.

O

N

NH2

H2O

2

N

H2O

NH

N

H2O

C18 packing
material

H2O

AdenineThymine

H2O

2

N

N
H

100% aqueous
H O
mobile phase H O

H2O
H2O

Image of C18 surface
hydration

Triart C18

Hybrid Silica Based Columns

Beforepump
stopping

2

1

tR5 = 14 min

3

H2

O

H2

O

H2

O

H2

O
H2

O

5

4

B111219E

No change in
retention time

98.0%
of initialretention time

After
pump
stopping

H2

O

H2

O
H2

O

Column: 5 μm, 150 X 4.6 mmI.D.
Eluent: 20 mM KH2PO4-K2HPO4 (pH 6.9)
Flow rate: 1.0 mL/min
Temperature : 37℃
Detection: UV at 254 nm

B111220A

0

4

8

12

16

min

The surface of Triart C18 is well-hydrated even after stopping pump.
This provides longer and stable retention time of polar nucleic bases.

Standard type C18
Brand I8
2

Beforepump
stopping

C18 for hydrophiliccompounds
Brand I931

4

tR5 = 7 min

5

1

2

3

B120110M

H2

O

H2

Remarkabledecrease inretention time82.2%
of initialretention time

63.5%
of initialretention time

O

H2

O
H2

O

H2

O

H2

O
H2

O

B120111A

B120112A

0

O

5

4

Remarkable decrease
in retention time
After
pump
stopping

H2

tR5 = 14 min

Poor retention

B120111I

Image of C18 surface
hydration

4

8

12

16

min

0

4

8

12

16

min

The surface of packing material is not fully hydrated. Compounds are not partitioned between
mobile phase and stationary phase, and therefore its retention becomes shorter.
Under the 100% aqueous mobile phase, conventional C18 columns generally show poorer performance (retention and peak shape) due to low surface
hydration caused by repulsion between aqueous mobile phase and hydrophobic bonded phase. There are several C18 columns that are compatible with
100% aqueous mobile phase in the market. Such columns exhibit excellent reproducibility and good retention ability of polar compounds achieved by sufficientsurface hydration. On the other hand, classical silica base resin and bonded phase are easily degraded under such highly aqueous condition. Those aqueouscompatible columns tend to have short lifetime.
To overcome the shortcomings of classical silica-based columns designed for highly aqueous compatibility, Triart C18 is a highly durable C18 column
with trifunctional bonding C18 phase on the organic/inorganic hybrid silica. Triart C18 is designed to retain both moderate hydrogen bonding capacity andhydrophobicity on the surface by optimizing bonded density of C18 phase. Its versatility is ideal for the first choice ODS column, and also applicable to analysesof polar compounds with 100% aqueous mobile phase condition.

50

Suitable for high sensitive LC/MS analysis【Analysis of tetracycline antibiotics using LC/MS】
Scan ES+

2

1

%

X 105
3

TIC

3

1.00e7

4

peak area

100

0

100

Scan ES+m/z 461%

1.80e6

Scan ES+00

m/z 479
1.80e6
0

1.

2

H3C
CH3 OH

HO

N

4

2.

CH3

HO

CH3

H 3C

N

O

OH

OH

O

Oxytetracycline(MW 460.4)

Cl HO

CH3

H3C

N

NH2

O

OH

O

OH

OH

O

CH3

H3C
OH

N

CH3
OH

NH2

O

Tetracycline(MW 444.4)

4.

CH3

OH

O

OH

OH

O

Triart C18 with its low bleeding characteristics is ideal
for high sensitivity analysis using LC/MS. In addition,
Triart C18’s surface inertness to basic compounds
and coordination compounds offers excellent andreproducible peak shape for quantitating difficult tochromatograph compounds.

min

OH

OH
NH2

OH

3.

CH3

OH

6

O

NH2
OH

Chlortetracycline(MW 478.8)

O

OH

OH

O

Doxycycline(MW 444.4)

1.0

O

Hybrid Silica Based Columns

Scan ES+%

0.5
concentration (µg/mL): YMC-Triart C18 5 μm
50 X 2.0 mmI.D.
Eluent: acetonitrile/water/formic acid (15/85/0.1)
Flow rate: 0.4 mL/min
Temperature : 40℃
Detection: ESI positive mode
Injection: 10 μL

1.80e6100

1Columnm/z 445%

R2 = 0.99993

2

0
0.0

0

100

Calibration curve of tetracycline(LC/MS m/z 445)

Minimizing strong solvent/sample loading effects【Improvement of loadability】
Sample dissolving solvent

Influence of injection volume on peak shapeacetonitrile

1

2

Injection volume
1.0 µL
1.5 µL2.0 µL3.0 µL

Triart C18

ColumnEluent1: 5 μm, 50 X 2.0 mmI.D. or 2.1 mmI.D.
: A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-0.5 min), 5-100%B (0.5-2.5 min)Flow rate: 0.4 mL/min
Temperature : 40℃
Detection: UV at 275 nm

Hybrid based C182

1

2

Silica based C18
Peak anomaly at 1.5 µL

1.

2.
H 3C
OH
O

H
N

H 3C

2.0

2.5

min

O

CH3
CH3

1.5

CH3

O

O

Propranolol (50 µg/mL)

N
H 3C

N
CH3

O

CH3

CH3

verapamil (50 µg/mL)

Triart C18 can tolerate larger injection volumes of samples containing solvents that have strong eluting ability (e.g., acetonitrile) while allowing for better peak
shape than conventional columns. This can be important for a sample pretreated with higher concentrations of organic solvent, crude reaction samples andpoorly soluble samples.

51

05

Hybrid Silica Based Columns

YMC-Triart C18 ExRSl C18 phase with high density bonding on organic/inorganic hybrid silica gell Excellent selectivity of isomers and structural analogsl Superior chemical durability

Pore size : 80 Å
Carbon content : 25%■ Usable pH range : 1-12
■ USP L1■■

Alternative selectivity to standard C18 columns
YMC-Triart C18 ExRS is C18 phase with high density bonding on organic/inorganic hybrid silica particles. In the case of
YMC-Triart C18 ExRS, hydrophobicity is high due to the high carbon loading (25%). This makes YMC-Triart C18 ExRS suitable for use withhydrophobic isomers and structural analogs. Given the superior chemical and physical durability of YMC-Triart C18 ExRS, chromatographers
are afforded additional flexibility in choosing separation conditions for both method development and routine column usage.
mAU

Hybrid Silica Based Columns

Comparison of120fundamental
separation
Hydrophobicityselectivity
index
Polarity indexα(3/ 7) 0.24mAU
80

Triart C18

k’(7) 9.95

2
3Polarity index
16α(3/547) 0.24
80
2
0 P120830N
3
1
40 0
10
5
6
54

Triart C18

0

Triart C18 ExRS

1. Amitriptyline (basic compound)
2. 8-Quinolinol (coordination compound)
3. Testosterone (polar compound)
4. Naphthalene (π-π interaction)
5. Ibuprofen (acidic compound)
6. Propylbenzene (hydrophobicity)
7. n-Butylbenzene (hydrophobicity)
8. o-Terphenyl (shape recognition ability)
9. Triphenylene (shape recognition ability)

k7’(7) 9.95

8 Shape9 recognition ability indexα(9/ 8) 1.21

20

15

7

25

8

9

20

25

min

P120830N

mAU 0

5

120

α(3/ 7) 0.13

mAU
80
120

Triart C18 ExRS

Shape recognition ability index(9/ 8) 1.21
Hydrophobicityα
index

120
40

2

3

1

40
80

2

0 F140929B
1
40 0

10

α(3/ 7) 0.13

3mink’(7) 18.18

4

5

6

10

4

7
20

6

ColumnEluent: 5 μm, 150 X 3.0 mmI.D.
: 20 mM KH2PO4-H3PO4(pH 3.1)/methanol (25/75)
Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 265 nm
Injection: 4 μLα(9/ 8) 1.56
8k’(7) 18.18
5

0

15

30

7

9

α(9/ 8) 1.56
40

min

8

9

F140929B

0

10

20

30

40

min

A mixture that consists of compounds with various characteristics is analyzed with Triart C18 and Triart C18 ExRS. Triart C18 ExRS has lower polarity andhigher hydrophobicity than the standard Triart C18 column. It also shows improved planar cognitive ability.

Ideal for separations of structural analogs
Vitamin D₂ / Vitamin D₃
1.

mAU
40

1

30
mAU
20
40
10
30
0
F1 4 0 9 1 6 A
20
0
10

Triart C18
Triart C18

0

H

2.

CH3

H

CH3

H3C

H3C

Rs (1, 2) = 0.87

1

2

H

H

4

6

8

10

Rs (1, 2) = 0.87

12

CH2

14 minHO

H
H

F1 4 0 9 1 6 A

2

4

6

8

1

30
mAU
20
40
10
30
0 F1 4 0 9 2 2 E
20
0
10

1

Rs (1, 2) = 1.59

2

4 Rs (1, 2)
6 = 1.59 8

2
10

12

Good resolution
2

Good resolution
10

12

14 min

HO

CH3

H

CH3

H3C
H
CH3

CH3

Vitamin D2(Ergocalciferol)

CH2
HO

H
H

CH3

Vitamin D3(Cholecalciferol)

CH2

CH2

14 min

H

H
CH3

H

2

CH3

H3C
H
CH3

CH3

CH3

2.

CH3

H

40

Triart C18 ExRS

H

Vitamin D₂ / Vitamin D₃
1.
CH3

mAU 0

Triart C18 ExRS

2

Vitamin D3
Vitamin D:25 μm, 150 X 3.0 mmI.D.
Column(Ergocalciferol)(Cholecalciferol)
Eluent: THF/acetonitrile (10/90)
Flow rate: 0.425 mL/min
Temperature : 30℃
Detection: UV at 265 nm
Injection: 4.25 μL (10 μg/mL)
HO

H

H

Triart C18 ExRS is effective
0 for separating of structural analogs. This feature is especially useful for separating pharmaceuticals with structurally similar
impurities.
0
2
4
6
8
10
12
14 minF1 4 0 9 2 2 E

pH 11.5, 40℃% of initial plate
theoreticalplate number% of initial theoreticalnumberImproved durability
120%% of initial theoretical plate number

Phosphate buffer (pH 11.5), 40℃

52

120%
100%
80%
60%
40%

100%
60%

Triart C18

Silica based C18

60%
20%

0%

Triart C18
Triart C8

Triart Hybrid
C8
based C1880%
40%

based20%
C18

0Triart C18 ExRS
Triart C18
TriartTriart
C18C8
ExRS
Triart C18 ExRS

120%
80%

40%
0%
Silica 0

20%
0%

pH
100% 11.5, 40℃

Column: 5 μm, 150 X 4.6 mmI.D.
Eluent: 50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)
Flow rate: 1.0 mL/min
Temperature : 40℃
Sample: benzyl alcohol

Hybrid based C18
Hybrid based C18
20

40

Silica based C18

60

80

100

Time (hr)High density bonding of C18 greatly contributes to improved chemical durability.
0

20

20
40

Time (hr)

40
60

60
80

Time (hr)

80
100

100

YMC-Triart C8
l Alternative to the more widely-used C18
l Usable over wide range of pH and temperaturel Ideal for separations of isomers or structural analogs

Pore size : 120 Å
Carbon content : 17%■ Usable pH range : 1-12
■ USP L7■■

Effective for fast analysis of compounds with low polarity or for separation of isomers
YMC-Triart C8 is a versatile column with excellent chemical durability that is equivalent to YMC-Triart C18. YMC-Triart C8 is suitablefor fast analysis of samples containing hydrophobic compounds that are strongly retained on C18 columns or samples containing
compounds with large difference in hydrophobicity.
In addition, its high bonded density provides high cognitive ability to separate compounds with structural differences. YMC-Triart C8 isalso ideal for the separation of isomers and structural analogs.

Hybrid Silica Based Columns

Comparable versatility to C18【Analysis of drugs】
Losartan potassium / hydrochlorothiazide

1

mAU
400

2

2.

1.

Cl
300

O
H2N

200

O
S

O

Cl

S

O

CH3

N
N

HO

NH

K
N

N
H

N
N

N

100

Hydrochlorothiazide

D111117A

0
0

ColumnEluent

5

10

15

20

25

Losartan potassium

min

Triart C8 has good chemical durability and peak shapes as goodas Triart C18. It is useful in various fields including pharmaceuticalproducts, food and natural products.

: YMC-Triart C8 5 μm, 150 X 4.0 mmI.D.
: A) phosphate buffer (pH 6.7)*/acetonitrile (93/7)
B) acetonitrile
0-8%B (0-12 min), 8-62%B (12-28 min)* Dissolve 1.25 g of KH2PO4 and 2.01 g of Na2HPO4・12H2O in 1000 mL of water

Flow rate: 1.0 mL/min
Temperature : 35℃
Detection: UV at 280 nm
Injection: 20 μL(The United States Pharmacopeia 34th; Assay)

Ideal for separations of isomers or structural analogs【Separation of positional isomers】
Triart C18

3

1
F130301E

0

5

10Triart C8

15

20

2

1

25

30

2
35

Baseline resolution in
shorter analysis time

3

F121228D

0

Column: 5 μm, 150 X 3.0 mmI.D.
Eluent: methanol/water (75/25)
Flow rate: 0.425 mL/min
Temperature : 30℃
Detection: UV at 254 nm

5

10

15

Terphenyl isomers
1.

o -Terphenyl

20

min

Triart C8 provides superior
resolution of Terphenyl isomers
to Triart C18. The higher bonded
density of C8 contributes torecognition of small difference
in structure though the elution
profile is similar between C18
and C8. Additionally, C8 phaseoffers shorter retention time thanC18 phase thanks to the lowhydrophobicity. These uniquecharacteristics are effective forfast analysis of isomers andcompounds with low polarity.

min

3.

2.

p -Terphenyl

m -Terphenyl

53

05

Hybrid Silica Based Columns

YMC-Triart Phenyll Unique selectivity due to π-π interactionl Ideal for separations of aromatic compounds or compounds having long conjugated systeml Excellent resolution without adsorption and tailing

Pore size : 120 Å
Carbon content : 17%■ Usable pH range : 1-10
■ USP L11■■

Effective for separation of compounds having long conjugated system by utilizing π-π interaction
YMC-Triart Phenyl is a phenylbutyl group bonded phase. Well balanced hydrophobic interaction and π-π interaction that is unique tophenyl group has been achieved by optimization of bonded density and spacer chain length (C4). Especially, compounds with aromaticring or long conjugated system tend to have strong retention. YMC-Triart Phenyl is ideal for separations of such isomers or structural
analogs. The surface modification common among YMC-Triart provides high durability and excellent peak shape without absorption.

Hybrid Silica Based Columns

Unique selectivity due to π-π interaction and superior peak shape without adsorption【Ideal for aromatic compounds and compounds having long conjugated system】
1

mAU

Triart C18

100
50
0

0

F130116H

2

C

mAU

Tf (1) = 1.56

150

C

10

B

0

1

A, B
D
6

4

4

6

8

10

12

14

16

18

Triart Phenyl

1

100
50

B

0

0

F130116G

2

mAU

4

6

C

0

10

12

14

16

18

A

F130117B

0

2

4

6

8

10

12

14

16

18

14

1

8

10

12

14

E
16

F
18

min

0

0

12

Good resolution with long retention

1, C, B
E

10

50

10

D

mAU

Tf (1) = -

8

B
6

8

C, B

100

C

mAU
10

1

150

Triart PFP

Tf (1) = 1.33

F

min

mAU
150

E

4

6

8

10

min

mAU

Tf (1) = 7.73

150

Silica based

Phenyl-Hexyl

Adsorption

100
50

C

B

0

0

F130117E

2

4

6

8

10

12

1
14

16

18

min

Brilliant Blue FCF and its impurities
1.

NaO3S

C2H5

C2H5

N

N

+

NaO3S

Brilliant Blue FCF
A - F : Structural analogs in Brilliant Blue FCF reagent

54

ColumnEluentFlow rate-

SO 3: 5 μm, 150 X 3.0 or 4.6 mmI.D.
: methanol/0.1% H3PO4 (45/55): 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
Temperature : 40℃
Detection: VIS at 630 nm

Brilliant Blue FCF of acidic triphenylmethane dye and its impurities (presumed to be byproducts having similar structure) can not be separated well with Triart C18. On the other
hand, they are retained well on Triart Phenyl, and excellent separation and peak shape
are obtained. Strong adsorption and poor resolution is observed on a commerciallyavailable phenylhexyl column. When it comes to separations of aromatic compounds orcompounds with long conjugated system, Triart Phenyl is more suitable than C18 due tostrong retention by π-π interaction.

YMC-Triart PFPl Alternative selectivity to C18/C8 due to unique polar interactionl Superior shape recognition ability/steric selectivity
l Ideal for separations of polar compounds or isomers

Pore size : 120 Å
Carbon content : 15%■ Usable pH range : 1-8■ USP L43■■

Effective for separation of polar compounds or isomers provided by unique polar interaction
YMC-Triart PFP is a pentafluorophenyl group bonded phase. The selectivity is unique due to various interactions such ashydrophobic, π-π, and dipole-dipole. YMC-Triart PFP is effective especially for improving separation of aromatic compounds, nitro
compounds, and compounds with halogen because the selectivity is very different from other columns.

Effective for separation of p
 olar compounds or isomers
mAU
40

Triart C18

1, 2, 420

0

3F121214C

0

5

10

15

20

25

min

20

25

min

mAU
40

Triart Phenyl

1, 2, 4320

0
0F121214D

5

10

mAU

Rs (1,2) = 1.19
Rs (2,3) = 1.54

20

0

5

10

mAU

2

3

4

15

20

25

min

15

20

25

min

1, 2

40

PFP

1

F121213E

0

Silica based

15

Good resolution

40

Triart PFP

Hybrid Silica Based Columns【Unique separation provided by various interactions】

3

20

4

0
0

F121213C

5

10Piperine cis-trans isomers
1.

2.

H
H

O
O

H

O

O

Chavicine(Z,Z-form)
ColumnEluentFlow rate: 5 μm, 150 X 3.0 or 4.6 mmI.D.
: acetonitrile/0.1% formic acid (40/60): 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
Temperature : 25℃
Detection: UV at 280 nm

3.

H
H

O
N

H

H

H

Isopiperine(Z,E-form)

H

O

O

N

4.

H

O

H

H
O

Isochavicine(E,Z-form)

N

H

H

O

O
O

N
H

H

Piperine(E,E-form)

Since the differences in hydrophobicity of cis-trans isomers of piperine, which is a pungent component
contained in pepper, are small, commonly used reversed-phase columns are not able to separate
them. However, Triart PFP can work well because Triart PFP can recognize minor charge localization
in a molecule due to various interactions such as π-π and dipole-dipole. It shows high selectivity forcompounds with small structural difference.

55

05

Hybrid Silica Based Columns

YMC-Triart Bio C4mAU

34 silica
l Wide400 pore columns with organic/inorganic
2 hybridand oligonucleotidesl Suitable for separation of biomolecules such as proteins
5
300 for separation of intact antibodies under high temperaturesl Useful6
l Good peak shapes with mobile phase suitable for LC/MS
200

Pore size : 300 Å
Usable pH range : 1-10
■ USP L26■■

1

Effective
for separation of biomolecules
100

YMC-Triart Bio C4 is an organic/inorganic hybrid silica based wide pore column suitable for separation of proteins. YMC-Triart Bio C40
V180125B33-49under high temperature. YMC-Triart Bio C4 achieves
can separate
proteins with molecular weight of up to 150,000 such as antibodies
2 peak shape35
6 phase 7containing
8 formic acid.
9min#excellent
even4 when using
mobileTherefore
it is effective for the high sensitive LC/MS
analysis. YMC-Triart Bio C4 is also useful for separation of peptides, oligonucleotides, and small molecules with high hydrophobicity
which are difficult to be separated with C18 columns.

Hybrid Silica Based Columns

Useful for separation of intact antibodies under high temperature
ColumnmAU
50
40
V180731F2

30

NIST mAb

V180731E2

20

Bevacizumab

V180731D2

10Adalimumab

V181107E2

Rituximab

V181107D2

0
0

1

Triart Bio C4 provides good peak shapes of intact
monoclonal antibodies of high molecular weights
under high temperature.

Trastuzumab
2

3

4

5

6

7

8

: YMC-Triart Bio C4 1.9 μm
50 X 2.1 mmI.D.
Eluent: A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
25-45%B (0-10 min)Flow rate: 0.4 mL/min
Temperature : 80℃
Detection: UV at 280 nm
Injection: 2 μL (0.5 mg/mL)

min#

9

Good peak shapes with mobile phase suitable for LC/MS
1. Cytochrome c (Horse heart)
2. Insulin (Bovine pancreas)
3. Transferrin (Human)
4. BSA
5. β-Lactoglobulin A (Bovine)
6. α-Chymotrypsinogen A (Bovine pancreas)

mAU

3
400

4

2

5

300

6

200

Column1

100
0V180125B33-49

2

3

4

5

6

7

8

9

min#

: YMC-Triart Bio C4 3 μm
150 X 3.0 mmI.D.
Eluent: A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
10-95%B (0-15 min)Flow rate: 0.4 mL/min
Temperature : 40℃
Detection: UV at 220 nm

Good peak shapes were obtained when analyzing proteins using Triart Bio C4 even with mobile phase containing formic acid.
The result shows that Triart Bio C4 is ideal for the high sensitive analysis using LC/MS.

Excellent chemical durability
pH 1 (1% TFA), 90℃
Triart C18

mAU
100% of initial retention

5090

Triart Bio C480
40
V180731F2

70
30
60
20
50

10
40
0

56

umber

0

NIST mAb

Silica based wide pore C4
V180731E2

Bevacizumab

The columns are kept in acetonitrile/water/TFA
V180731D2(10/90/1,
pH 1) at 90℃, and tested
for performance every 20 hours

Generally, improvement of peptide and protein separation is expected under

Adalimumaba high temperature condition using a mobile phase containing TFA, although

V181107E2

0
20
V181107D2

1

2

40

60

80

shorter lifetime of conventional columns becomes a problem under such a
Rituximab

100

4

5

6

7

8

condition. Since Triart is based on organic/inorganic hybrid particle, which has
its innovative surface modification, it keeps excellent durability even underharsh conditions such as 1% TFA and/or 90℃ .

Trastuzumab

Time (hr)3Test conditions
Column: 5 μm, 150 X 3.0 mmI.D. for C4
5 μm, 50 X 2.0 mmI.D. for C18
Eluent: acetonitrile/water (60/40)
Flow rate: 0.4 mL/min for 3.0 mmI.D.
0.2 mL/min for 2.0 mmI.D.
Temperature : 37℃
Sample: butyl benzoate

9

min#

YMC-Triart Diol-HILICl Ideal for separations of highly polar compounds, which are hardly retained ona reversed-phase columnl Superior durability and usable under wide range of mobile phase conditionsl Excellent reproducibility with less ionic adsorption

Pore size : 120 Å
Carbon content : 12%■ Usable pH range : 2-10
■ USP L20■■

Effective for separation of highly polar compounds
YMC-Triart Diol-HILIC is a HILIC (hydrophilic interaction chromatography) column based on an organic/inorganic hybrid particle
synthesized with dihydroxypropyl group. YMC-Triart Diol-HILIC is ideal for a separation of polar and hydrophilic compounds which
are not retained on reversed-phase (C18, C8, and others) chromatography. YMC-Triart Diol-HILIC based on organic/inorganic hybrid
particle provides excellent durability and is usable across a wide pH range. Low nonspecific adsorption provided by ionically neutral
dihydroxypropyl group offers quantitative analysis with high reproducibility.

【Comparison of reversed-phase and HILIC separations】
Reversed-phase mode

Triart C18

mV

1.

HILIC mode

Triart Diol-HILIC
mV

20 mM KH2PO4-H3PO4 (pH 2.5)

HO H
HO
HO

100 mM HCOOH-HCOONH4 (pH 3.3)/acetonitrile (10/90)

O

O
OH

Erythorbic acid
(D-Isoascorbic acid)

2.

HO H
HO
HO

O

O
OH

L-Ascorbic acid

2

1

200

Changing
separation mode

1

2

100

100

0

Column: 5 μm, 150 X 3.0 mmI.D.
Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 254 nm
Injection: 4 μL200

0F120425A

0.0

2.5

5.0 min

B120427L

0.0

2.5

5.0

7.5

10.0 minHybrid Silica Based Columns

Ideal for separation of highly polar compounds which are hardly retained on a reversed-phase column

Triart C18 (reversed-phase) shows very weakretention and poor resolution of L-ascorbic acidand its stereoisomer (erythorbic acid) even with a
100% aqueous mobile phase. On the other hand,
Triart Diol-HILIC shows strong retention and better
resolution of these compounds with a mobile phase
containing 90% organic solvent.

Excellent durability and reproducibility in wide range of conditions【Extended lifetime in chemically challenging condition】
ColumnEluentDurability in high pH（pH 11, 50℃）
Triart Diol-HILIC

100%% of initial retention

Cytosine
200 hr80%

150 hr60%

100 hr

40%

0%

50 hr

Silica based Diol
for HILIC

20%
0

50

100

150

Time (hr)initial

200

4

5

6

7

min

Application (F121012A)
Application
Water-soluble vitamins

4

7. L-Ascorbic acid8. Nicotinic acid
9. 2-O-α-D-Glucopyranosyl-L-ascorbic acid
3. Pyridoxine hydrochloride
1. Caffeine 4. Riboflavin
7. L-Ascorbic
acidacid 2-glucoside)(Ascorbic
2. Nicotinamide
8. Nicotinicacid hydrochloride
5. Orotic acid
10. Thiamine
6. Erythorbic
acid (D-Isoascorbic 9.
acid)
11. Cyanocobalamin
3. Pyridoxinehydrochloride
2-O-α-D-Glucopyranosyl-L-ascorbicacid
1. Caffeine

2. Nicotinamide
Water-soluble
vitamins

9

4

mAU

150

1

2

9

11

100

150

1

5

2

7
50

100

6

5
7

8

11

10

4. Riboflavin(Ascorbic acid 2-glucoside)
5. Oroticacid
10. Thiamine
Column: YMC-Triart Diol-HILIC(5 μm, 120hydrochlorideÅ), 150 X 3.0 mmI.D.
6. Erythorbicacid) 11.
Eluentacid (D-Isoascorbic: A) acetonitrile/200
mMCyanocobalamin
HCOOH-HCOONH4 (pH 3.6)/water (90/5/5)

3

8

0

50

6

0

2

10
4

6

8

10

12

10

12

14

16

18

14

16

3
0
0

2

4

6

8

Triart Diol-HILIC provides highly reproducible
separations even in high pH (pH 11) and at hightemperature (50℃). Triart Diol-HILIC shows
extremely long column lifetime even in suchchemically harsh condition compared to conventional
silica-based Diol column.

(F121012A)

Application (F121012A)
mAU: 5 μm, 150 X 4.6 mmI.D.
: acetonitrile/water/NH3(90/10/0.1) pH 11.3
Flow rate: 1.0 mL/min
Temperature : 50℃
Sample: cytosine

20 min#18B) acetonitrile/200 mM HCOOH-HCOONH4 (pH 3.6)/water (50/5/45)
0-75%B (0-20 min)Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 254 nm
Injection: 4 μL (50 μg/mL)

20 min#57

05

Hybrid Silica Based Columns
Ordering Information –Columns–
Pressure limit : 100 MPa for 1.9 μm, 45 MPa for 3 μm and 5 μm; Style of endfitting : Parker style (UPLC compatible)
Particle Column size
size inner diameter(μm) X length (mm)1.9

Hybrid Silica Based Columns
3

5

Triart C18(120 Å)Triart C18 ExRS
(80 Å)

Triart C8(120 Å)Triart PFP(120 Å)Triart Bio C4(300 Å)Triart Diol-HILIC(120 Å)2.0 X 20

TA12SP9-0202PT

TAR08SP9-0202PT

TO12SP9-0202PT

TPH12SP9-0202PT

TPF12SP9-0202PT——

2.0 X 30

TA12SP9-0302PT

TAR08SP9-0302PT

TO12SP9-0302PT

TPH12SP9-0302PT

TPF12SP9-0302PT——

2.0 X 50

TA12SP9-0502PT

TAR08SP9-0502PT

TO12SP9-0502PT

TPH12SP9-0502PT

TPF12SP9-0502PT—

TDH12SP9-0502PT

2.0 X 75

TA12SP9-L502PT

TAR08SP9-L502PT

TO12SP9-L502PT

TPH12SP9-L502PT

TPF12SP9-L502PT—

TDH12SP9-L502PT
TDH12SP9-1002PT

2.0 X 100

TA12SP9-1002PT

TAR08SP9-1002PT

TO12SP9-1002PT

TPH12SP9-1002PT

TPF12SP9-1002PT—

2.0 X 150

TA12SP9-1502PT

TAR08SP9-1502PT

TO12SP9-1502PT

TPH12SP9-1502PT

TPF12SP9-1502PT——

2.1 X 20

TA12SP9-02Q1PT

TAR08SP9-02Q1PT

TO12SP9-02Q1PT

TPH12SP9-02Q1PT

TPF12SP9-02Q1PT

TB30SP9-02Q1PT—

2.1 X 30

TA12SP9-03Q1PT

TAR08SP9-03Q1PT

TO12SP9-03Q1PT

TPH12SP9-03Q1PT

TPF12SP9-03Q1PT

TB30SP9-03Q1PT—

2.1 X 50

TA12SP9-05Q1PT

TAR08SP9-05Q1PT

TO12SP9-05Q1PT

TPH12SP9-05Q1PT

TPF12SP9-05Q1PT

TB30SP9-05Q1PT

TDH12SP9-05Q1PT

2.1 X 75

TA12SP9-L5Q1PT

TAR08SP9-L5Q1PT

TO12SP9-L5Q1PT

TPH12SP9-L5Q1PT

TPF12SP9-L5Q1PT

TB30SP9-L5Q1PT

TDH12SP9-L5Q1PT

2.1 X 100

TA12SP9-10Q1PT

TAR08SP9-10Q1PT

TO12SP9-10Q1PT

TPH12SP9-10Q1PT

TPF12SP9-10Q1PT

TB30SP9-10Q1PT

TDH12SP9-10Q1PT

2.1 X 150

TA12SP9-15Q1PT

TAR08SP9-15Q1PT

TO12SP9-15Q1PT

TPH12SP9-15Q1PT

TPF12SP9-15Q1PT

TB30SP9-15Q1PT—

3.0 X 50

TA12SP9-0503PT

TAR08SP9-0503PT

TO12SP9-0503PT

TPH12SP9-0503PT

TPF12SP9-0503PT

TB30SP9-0503PT

TDH12SP9-0503PT

3.0 X 75

TA12SP9-L503PT

TAR08SP9-L503PT

TO12SP9-L503PT

TPH12SP9-L503PT

TPF12SP9-L503PT

TB30SP9-L503PT

TDH12SP9-L503PT

3.0 X 100

TA12SP9-1003PT

TAR08SP9-1003PT

TO12SP9-1003PT

TPH12SP9-1003PT

TPF12SP9-1003PT

TB30SP9-1003PT

TDH12SP9-1003PT

3.0 X 150

TA12SP9-1503PT

TAR08SP9-1503PT

TO12SP9-1503PT

TPH12SP9-1503PT

TPF12SP9-1503PT

TB30SP9-1503PT—

2.1 X 20

TA12S03-02Q1PTHTAR08S03-02Q1PTH

TO12S03-02Q1PTH

TPH12S03-02Q1PTH

TPF12S03-02Q1PTH

TB30S03-02Q1PTH

TDH12S03-02Q1PTH
TDH12S03-H3Q1PTH

2.1 X 33

TA12S03-H3Q1PTHTAR08S03-H3Q1PTH

TO12S03-H3Q1PTH

TPH12S03-H3Q1PTH TPF12S03-H3Q1PTH

TB30S03-H3Q1PTH

2.1 X 50

TA12S03-05Q1PTHTAR08S03-05Q1PTH

TO12S03-05Q1PTH

TPH12S03-05Q1PTH

TPF12S03-05Q1PTH

TB30S03-05Q1PTH

TDH12S03-05Q1PTH

2.1 X 75

TA12S03-L5Q1PTHTAR08S03-L5Q1PTH

TO12S03-L5Q1PTH

TPH12S03-L5Q1PTH

TPF12S03-L5Q1PTH

TB30S03-L5Q1PTH

TDH12S03-L5Q1PTH

2.1 X 100

TA12S03-10Q1PTHTAR08S03-10Q1PTH

TO12S03-10Q1PTH

TPH12S03-10Q1PTH

TPF12S03-10Q1PTH

TB30S03-10Q1PTH

TDH12S03-10Q1PTH

2.1 X 150

TA12S03-15Q1PTHTAR08S03-15Q1PTH

TO12S03-15Q1PTH

TPH12S03-15Q1PTH

TPF12S03-15Q1PTH

TB30S03-15Q1PTH

TDH12S03-15Q1PTH

3.0 X 50

TA12S03-0503PTH

TAR08S03-0503PTH

TO12S03-0503PTH

TPH12S03-0503PTH

TPF12S03-0503PTH

TB30S03-0503PTH

TDH12S03-0503PTH

3.0 X 75

TA12S03-L503PTH

TAR08S03-L503PTH

TO12S03-L503PTH

TPH12S03-L503PTH

TPF12S03-L503PTH

TB30S03-L503PTH

TDH12S03-L503PTH

3.0 X 100

TA12S03-1003PTH

TAR08S03-1003PTH

TO12S03-1003PTH

TPH12S03-1003PTH

TPF12S03-1003PTH

TB30S03-1003PTH

TDH12S03-1003PTH

3.0 X 150

TA12S03-1503PTH

TAR08S03-1503PTH

TO12S03-1503PTH

TPH12S03-1503PTH

TPF12S03-1503PTH

TB30S03-1503PTH

TDH12S03-1503PTH
TDH12S03-H346PTH

4.6 X 33

TA12S03-H346PTH

TAR08S03-H346PTH

TO12S03-H346PTH

TPH12S03-H346PTH

TPF12S03-H346PTH

TB30S03-H346PTH

4.6 X 50

TA12S03-0546PTH

TAR08S03-0546PTH

TO12S03-0546PTH

TPH12S03-0546PTH

TPF12S03-0546PTH

TB30S03-0546PTH

TDH12S03-0546PTH

4.6 X 75

TA12S03-L546PTH

TAR08S03-L546PTH

TO12S03-L546PTH

TPH12S03-L546PTH

TPF12S03-L546PTH

TB30S03-L546PTH

TDH12S03-L546PTH

4.6 X 100

TA12S03-1046PTH

TAR08S03-1046PTH

TO12S03-1046PTH

TPH12S03-1046PTH

TPF12S03-1046PTH

TB30S03-1046PTH

TDH12S03-1046PTH

4.6 X 150

TA12S03-1546PTH

TAR08S03-1546PTH

TO12S03-1546PTH

TPH12S03-1546PTH

TPF12S03-1546PTH

TB30S03-1546PTH

TDH12S03-1546PTH

4.6 X 250

TA12S03-2546PTH

TAR08S03-2546PTH

TO12S03-2546PTH

TPH12S03-2546PTH

TPF12S03-2546PTH

TB30S03-2546PTH

TDH12S03-2546PTH

2.1 X 20

TA12S05-02Q1PTHTAR08S05-02Q1PTH

TO12S05-02Q1PTH

TPH12S05-02Q1PTH

TPF12S05-02Q1PTH

TB30S05-02Q1PTH

TDH12S05-02Q1PTH
TDH12S05-H3Q1PTH

2.1 X 33

TA12S05-H3Q1PTHTAR08S05-H3Q1PTH

TO12S05-H3Q1PTH

TPH12S05-H3Q1PTH TPF12S05-H3Q1PTH

TB30S05-H3Q1PTH

2.1 X 50

TA12S05-05Q1PTHTAR08S05-05Q1PTH

TO12S05-05Q1PTH

TPH12S05-05Q1PTH

TPF12S05-05Q1PTH

TB30S05-05Q1PTH

TDH12S05-05Q1PTH

2.1 X 75

TA12S05-L5Q1PTHTAR08S05-L5Q1PTH

TO12S05-L5Q1PTH

TPH12S05-L5Q1PTH

TPF12S05-L5Q1PTH

TB30S05-L5Q1PTH

TDH12S05-L5Q1PTH

2.1 X 100

TA12S05-10Q1PTHTAR08S05-10Q1PTH

TO12S05-10Q1PTH

TPH12S05-10Q1PTH

TPF12S05-10Q1PTH

TB30S05-10Q1PTH

TDH12S05-10Q1PTH

2.1 X 150

TA12S05-15Q1PTHTAR08S05-15Q1PTH

TO12S05-15Q1PTH

TPH12S05-15Q1PTH

TPF12S05-15Q1PTH

TB30S05-15Q1PTH

TDH12S05-15Q1PTH

3.0 X 50

TA12S05-0503PTH

TAR08S05-0503PTH

TO12S05-0503PTH

TPH12S05-0503PTH

TPF12S05-0503PTH

TB30S05-0503PTH

TDH12S05-0503PTH

3.0 X 75

TA12S05-L503PTH

TAR08S05-L503PTH

TO12S05-L503PTH

TPH12S05-L503PTH

TPF12S05-L503PTH

TB30S05-L503PTH

TDH12S05-L503PTH

3.0 X 100

TA12S05-1003PTH

TAR08S05-1003PTH

TO12S05-1003PTH

TPH12S05-1003PTH

TPF12S05-1003PTH

TB30S05-1003PTH

TDH12S05-1003PTH

3.0 X 150

TA12S05-1503PTH

TAR08S05-1503PTH

TO12S05-1503PTH

TPH12S05-1503PTH

TPF12S05-1503PTH

TB30S05-1503PTH

TDH12S05-1503PTH

4.0 X 150

TA12S05-1504PTH

TAR08S05-1504PTH

TO12S05-1504PTH

TPH12S05-1504PTH

TPF12S05-1504PTH

TB30S05-1504PTH

TDH12S05-1504PTH

4.0 X 250

TA12S05-2504PTH

TAR08S05-2504PTH

TO12S05-2504PTH

TPH12S05-2504PTH

TPF12S05-2504PTH

TB30S05-2504PTH

TDH12S05-2504PTH

4.6 X 33

TA12S05-H346PTH

TAR08S05-H346PTH

TO12S05-H346PTH

TPH12S05-H346PTH

TPF12S05-H346PTH

TB30S05-H346PTH

TDH12S05-H346PTH

4.6 X 50

TA12S05-0546PTH

TAR08S05-0546PTH

TO12S05-0546PTH

TPH12S05-0546PTH

TPF12S05-0546PTH

TB30S05-0546PTH

TDH12S05-0546PTH

4.6 X 75

TA12S05-L546PTH

TAR08S05-L546PTH

TO12S05-L546PTH

TPH12S05-L546PTH

TPF12S05-L546PTH

TB30S05-L546PTH

TDH12S05-L546PTH

4.6 X 100

TA12S05-1046PTH

TAR08S05-1046PTH

TO12S05-1046PTH

TPH12S05-1046PTH

TPF12S05-1046PTH

TB30S05-1046PTH

TDH12S05-1046PTH

4.6 X 150

TA12S05-1546PTH

TAR08S05-1546PTH

TO12S05-1546PTH

TPH12S05-1546PTH

TPF12S05-1546PTH

TB30S05-1546PTH

TDH12S05-1546PTH

4.6 X 250

TA12S05-2546PTH

TAR08S05-2546PTH

TO12S05-2546PTH

TPH12S05-2546PTH

TPF12S05-2546PTH

TB30S05-2546PTH

TDH12S05-2546PTH＊ See p.114 for preparative columns other than those listed above.

58

Triart Phenyl(120 Å)Ordering Information –Columns–
Pressure limit : 10-25 MPa, depending on dimensions; Style of endfitting : Waters (W) style
Particle
size
(μm)

3

Triart C18(120 Å)Triart C8(120 Å)Triart Phenyl(120 Å)Triart PFP(120 Å)Triart Diol-HILIC(120 Å)2.0 X 20

TA12S03-0202WT

TO12S03-0202WT

TPH12S03-0202WTTPF12S03-0202WT

TDH12S03-0202WT

2.0 X 30

TA12S03-0302WTTO12S03-0302WTTPH12S03-0302WTTPF12S03-0302WT

TDH12S03-0302WT

2.0 X 50

TA12S03-0502WT

TO12S03-0502WT

TPH12S03-0502WTTPF12S03-0502WT

TDH12S03-0502WT

2.0 X 75

TA12S03-L502WT

TO12S03-L502WT

TPH12S03-L502WTTPF12S03-L502WT

TDH12S03-L502WT

2.0 X 100

TA12S03-1002WT

TO12S03-1002WT

TPH12S03-1002WT

TPF12S03-1002WT

TDH12S03-1002WT

2.0 X 150

TA12S03-1502WT

TO12S03-1502WT

TPH12S03-1502WTTPF12S03-1502WT

TDH12S03-1502WT

3.0 X 50

TA12S03-0503WT

TO12S03-0503WT

TPH12S03-0503WTTPF12S03-0503WT

TDH12S03-0503WT

3.0 X 75

TA12S03-L503WT

TO12S03-L503WT

TPH12S03-L503WTTPF12S03-L503WT

TDH12S03-L503WT

3.0 X 100

TA12S03-1003WT

TO12S03-1003WT

TPH12S03-1003WT

TPF12S03-1003WT

TDH12S03-1003WT

3.0 X 150

TA12S03-1503WT

TO12S03-1503WT

TPH12S03-1503WTTPF12S03-1503WT

TDH12S03-1503WT

4.6 X 35

TA12S03-H546WTTO12S03-H546WT

TPH12S03-H546WTTPF12S03-H546WT

TDH12S03-H546WT

4.6 X 50

TA12S03-0546WTTO12S03-0546WT

TPH12S03-0546WTTPF12S03-0546WT

TDH12S03-0546WT

4.6 X 75

TA12S03-L546WTTO12S03-L546WT

TPH12S03-L546WTTPF12S03-L546WT

TDH12S03-L546WT

4.6 X 100

TA12S03-1046WT

TO12S03-1046WT

TPH12S03-1046WTTPF12S03-1046WT

TDH12S03-1046WT

4.6 X 150

TA12S03-1546WTTO12S03-1546WT

TPH12S03-1546WTTPF12S03-1546WT

TDH12S03-1546WT

4.6 X 250

TA12S03-2546WTTO12S03-2546WT

TPH12S03-2546WTTPF12S03-2546WT

TDH12S03-2546WT

2.0 X 20

TA12S05-0202WT

TO12S05-0202WT

TPH12S05-0202WTTPF12S05-0202WT

TDH12S05-0202WT

2.0 X 30

TA12S05-0302WTTO12S05-0302WTTPH12S05-0302WTTPF12S05-0302WT

TDH12S05-0302WT

2.0 X 50

TA12S05-0502WT

TO12S05-0502WT

TPH12S05-0502WTTPF12S05-0502WT

TDH12S05-0502WT

2.0 X 75

TA12S05-L502WT

TO12S05-L502WT

TPH12S05-L502WTTPF12S05-L502WT

TDH12S05-L502WT

2.0 X 100

TA12S05-1002WT

TO12S05-1002WT

TPH12S05-1002WT

TPF12S05-1002WT

TDH12S05-1002WT

2.0 X 150

TA12S05-1502WT

TO12S05-1502WT

TPH12S05-1502WTTPF12S05-1502WT

TDH12S05-1502WT

3.0 X 50

TA12S05-0503WT

TO12S05-0503WT

TPH12S05-0503WTTPF12S05-0503WT

TDH12S05-0503WT

3.0 X 75

TA12S05-L503WT

TO12S05-L503WT

TPH12S05-L503WTTPF12S05-L503WT

TDH12S05-L503WT

3.0 X 100

TA12S05-1003WT

TO12S05-1003WT

TPH12S05-1003WT

TPF12S05-1003WT

TDH12S05-1003WT

3.0 X 150

TA12S05-1503WT

TO12S05-1503WT

TPH12S05-1503WTTPF12S05-1503WT

TDH12S05-1503WT

4.0 X 150

TA12S05-1504WTTO12S05-1504WT

TPH12S05-1504WTTPF12S05-1504WT

TDH12S05-1504WT

4.0 X 250

TA12S05-2504WTTO12S05-2504WT

TPH12S05-2504WTTPF12S05-2504WT

TDH12S05-2504WT

4.6 X 35

TA12S05-H546WTTO12S05-H546WT

TPH12S05-H546WTTPF12S05-H546WT

TDH12S05-H546WT

4.6 X 50

TA12S05-0546WTTO12S05-0546WT

TPH12S05-0546WTTPF12S05-0546WT

TDH12S05-0546WT

4.6 X 75

TA12S05-L546WTTO12S05-L546WT

TPH12S05-L546WTTPF12S05-L546WT

TDH12S05-L546WT

4.6 X 100

TA12S05-1046WT

TO12S05-1046WT

TPH12S05-1046WTTPF12S05-1046WT

TDH12S05-1046WT

4.6 X 150

TA12S05-1546WTTO12S05-1546WT

TPH12S05-1546WTTPF12S05-1546WT

TDH12S05-1546WT

4.6 X 250

TA12S05-2546WTTO12S05-2546WT

TPH12S05-2546WTTPF12S05-2546WT

TDH12S05-2546WT

6.0 X 150

TA12S05-1506WTTO12S05-1506WT

TPH12S05-1506WTTPF12S05-1506WT—

6.0 X 250

TA12S05-2506WTTO12S05-2506WT

TPH12S05-2506WTTPF12S05-2506WT—

10 X 150

TA12S05-1510WTTO12S05-1510WTTPH12S05-1510WTTPF12S05-1510WT—

10 X 250

TA12S05-2510WTTO12S05-2510WTTPH12S05-2510WTTPF12S05-2510WT—

Hybrid Silica Based Columns

5

Column size
inner diameter X
length (mm)＊ See p.114 for preparative columns other than those listed above.

59

05

Hybrid Silica Based Columns
Ordering Information –Columns–
Metal free columns
Pressure limit : 100 MPa for 1.9 μm, 45 MPa for 3 μm and 5 μm; Style of endfitting : Parker style (UPLC compatible)
Particle Column size
size inner diameter(μm) X length (mm)1.9

3Hybrid Silica Based Columns

5

Triart C18(120 Å)Triart C18 ExRS
(80 Å)

Triart C8(120 Å)Triart Phenyl(120 Å)Triart PFP(120 Å)Triart Bio C4(300 Å)Triart Diol-HILIC(120 Å)2.1 X 50

TA12SP9-05Q1PTPTAR08SP9-05Q1PTPTO12SP9-05Q1PTPTPH12SP9-05Q1PTPTPF12SP9-05Q1PTPTB30SP9-05Q1PTPTDH12SP9-05Q1PTP

2.1 X 100

TA12SP9-10Q1PTPTAR08SP9-10Q1PTPTO12SP9-10Q1PTPTPH12SP9-10Q1PTPTPF12SP9-10Q1PTPTB30SP9-10Q1PTPTDH12SP9-10Q1PTP

2.1 X 150

TA12SP9-15Q1PTPTAR08SP9-15Q1PTPTO12SP9-15Q1PTPTPH12SP9-15Q1PTPTPF12SP9-15Q1PTPTB30SP9-15Q1PTPTDH12SP9-15Q1PTP

2.1 X 50

TA12S03-05Q1PTPTAR08S03-05Q1PTPTO12S03-05Q1PTPTPH12S03-05Q1PTPTPF12S03-05Q1PTPTB30S03-05Q1PTPTDH12S03-05Q1PTP

2.1 X 100

TA12S03-10Q1PTPTAR08S03-10Q1PTPTO12S03-10Q1PTPTPH12S03-10Q1PTPTPF12S03-10Q1PTPTB30S03-10Q1PTPTDH12S03-10Q1PTP

2.1 X 150

TA12S03-15Q1PTPTAR08S03-15Q1PTPTO12S03-15Q1PTPTPH12S03-15Q1PTPTPF12S03-15Q1PTPTB30S03-15Q1PTPTDH12S03-15Q1PTP

4.6 X 50

TA12S03-0546PTPTAR08S03-0546PTPTO12S03-0546PTP

TPH12S03-0546PTPTPF12S03-0546PTPTB30S03-0546PTP

TDH12S03-0546PTP

4.6 X 100

TA12S03-1046PTPTAR08S03-1046PTPTO12S03-1046PTP

TPH12S03-1046PTPTPF12S03-1046PTPTB30S03-1046PTP

TDH12S03-1046PTP

4.6 X 150

TA12S03-1546PTPTAR08S03-1546PTPTO12S03-1546PTP

TPH12S03-1546PTPTPF12S03-1546PTPTB30S03-1546PTP

TDH12S03-1546PTP

2.1 X 50

TA12S05-05Q1PTPTAR08S05-05Q1PTPTO12S05-05Q1PTPTPH12S05-05Q1PTPTPF12S05-05Q1PTPTB30S05-05Q1PTPTDH12S05-05Q1PTP

2.1 X 100

TA12S05-10Q1PTPTAR08S05-10Q1PTPTO12S05-10Q1PTPTPH12S05-10Q1PTPTPF12S05-10Q1PTPTB30S05-10Q1PTPTDH12S05-10Q1PTP

2.1 X 150

TA12S05-15Q1PTPTAR08S05-15Q1PTPTO12S05-15Q1PTPTPH12S05-15Q1PTPTPF12S05-15Q1PTPTB30S05-15Q1PTPTDH12S05-15Q1PTP

4.6 X 50

TA12S05-0546PTPTAR08S05-0546PTPTO12S05-0546PTP

TPH12S05-0546PTPTPF12S05-0546PTPTB30S05-0546PTP

TDH12S05-0546PTP

4.6 X 100

TA12S05-1046PTPTAR08S05-1046PTPTO12S05-1046PTP

TPH12S05-1046PTPTPF12S05-1046PTPTB30S05-1046PTP

TDH12S05-1046PTP

4.6 X 150

TA12S05-1546PTPTAR08S05-1546PTPTO12S05-1546PTP

TPH12S05-1546PTPTPF12S05-1546PTPTB30S05-1546PTP

TDH12S05-1546PTP

Ordering Information –Guard Cartridge Columns–
EXP ® Guard Cartridge Columns (3/pack)
Particle Column size
size inner diameter(μm) X length (mm)1.9

Triart C18(120 Å)Triart C18 ExRS
(80 Å)

Triart C8(120 Å)Triart Phenyl(120 Å)Triart PFP(120 Å)Triart Bio C4(300 Å)2.1 X 5TA12SP9-E5Q1CC

TAR08SP9-E5Q1CC

TO12SP9-E5Q1CC

TPH12SP9-E5Q1CC

TPF12SP9-E5Q1CC

TB30SP9-E5Q1CC

3.0 X 5TA12SP9-E503CC

TAR08SP9-E503CC

TO12SP9-E503CC

TPH12SP9-E503CC

TPF12SP9-E503CC

TB30SP9-E503CC*EXP®Guard cartridge holder required, part no. XPCHUHP.
*EXP is registered trademark of Optimize Technologies, Inc.

Guard Cartridge Columns
Particle Column size
size inner diameter /pack(μm) X length (mm)2.1 X 103

3.0 X 10

5

4.0 X 10
2.1 X 10

5

3.0 X 10

5

4.0 X 10
10 X 10

2

Triart C18(120 Å)Triart C18 ExRS
(80 Å)

Triart C8(120 Å)Triart Phenyl(120 Å)Triart PFP(120 Å)Triart Bio C4(300 Å)Triart Diol-HILIC(120 Å)TA12S03-01Q1GC

TAR08S03-01Q1GC

TO12S03-01Q1GC

TPH12S03-01Q1GC

TPF12S03-01Q1GC

TB30S03-01Q1GC

TDH12S03-01Q1GC

TA12S03-0103GC

TAR08S03-0103GC

TO12S03-0103GC

TPH12S03-0103GC

TPF12S03-0103GC

TB30S03-0103GC

TDH12S03-0103GC

TA12S03-0104GC

TAR08S03-0104GC

TO12S03-0104GC

TPH12S03-0104GC

TPF12S03-0104GC

TB30S03-0104GC

TDH12S03-0104GC

TA12S05-01Q1GC

TAR08S05-01Q1GC

TO12S05-01Q1GC

TPH12S05-01Q1GC

TPF12S05-01Q1GC

TB30S05-01Q1GC

TDH12S05-01Q1GC

TA12S05-0103GC

TAR08S05-0103GC

TO12S05-0103GC

TPH12S05-0103GC

TPF12S05-0103GC

TB30S05-0103GC

TDH12S05-0103GC

TA12S05-0104GC

TAR08S05-0104GC

TO12S05-0104GC

TPH12S05-0104GC

TPF12S05-0104GC

TB30S05-0104GC

TDH12S05-0104GC

TA12S05-0110CC

TAR08S05-0110CC

TO12S05-0110CC

TPH12S05-0110CC

TPF12S05-0110CC

TB30S05-0110CC—*Guard cartridge holder required, part no. XPGCH-Q1 for 2.1-4.0 mmI.D. and XPCHSPW1 for 10 mmI.D.

60