Hybrid silica based C18 column that is stable under the use of 100% aqueous
mobile phase - YMC-Triart C18 Q130215AE
Retention stability under 100% aqueous mobile phase
Under the 100% aqueous mobile phase, conventional C18 columns generally show poorer
performance (retention and peak shape) due to low surface hydration caused by repulsion
between aqueous mobile phase and hydrophobic boned phase. Several columns that arecompatible with 100% aqueous mobile phase in the market. Such columns exhibit
excellent reproducibility and good retention ability of polar compounds achieved bysuﬃcient surface hydration. On the other hand, classical silica base resin and bonded phaseare easily degraded under such highly aqueous condition. Those aqueous compatible
columns tend to have short lifetime.
YMC-Triart C18 is a highly durable C18 column with polymerically bonded C18 phase on
the organic/inorganic hybrid silica. YMC-Triart C18 is designed to retain both moderatehydrogen bonding capacity and hydrophobicity on the surface by optimizing bonded
density of C18 phase. Its versatility is ideal for the ﬁrst choice ODS column, and also
applicable to analyses of polar compounds with 100% aqueous mobile phase condition.

Image of C18 surface
100 % aqueousmobile phase

H2O

H2O

H2O

H2O
H2O
H2O
H2O

Hybrid silica
based C18

H2O
H2O

Excellent retention stability of Triart C18

1Afterpumpstopping

O
H2O
H2O
H2O
H2O
H254

B1112 19E

O
H2

tR 5 = 1432

Beforepumpstopping

Nucleic bases

Image of C18 surface hydration

YMC-Triart C18

No change
in retention time!

OH2O
H2

98.0% ofinitial retention
B1122 0A

51510minThe surface of YMC-Triart C18 is well-hydrated even after stopping pump.
This provides longer and stable retention time of polar nucleic bases.

ODS for hydrophilic compounds
Brand I9Standard type ODS
Brand I8Beforepumpstopping

311tR 5 = 754O
H2322

Image of C18 surface hydration

OH2ColumnO
H2O
H282.2% of
initial retention

63.5% ofinitial retention
B1201 11A

B1201 12A0O
H2O
H25B1201 10M

AfterpumpstoppingO
H2tR 5 = 144B1201 11IOH251015: 5 µm, 150 X 4.6 mmI.D.
or 5 µm, 150 X 3.0 mmI.D.
Eluent: 20 mM KH2PO4-K2HPO4 (pH 6.9)
Flow rate: 1.0 mL/min for 4.6 mmI.D.
0.425 mL/min for 3.0 mmI.D.
Temperature : 37℃Detection: UV at 254 nm

051015

In contrast to Triart C18, Brand I8 shows shorter retention and poor retention stability
after stopping pump. Brand I9, which is designed for polar compounds and claimed
that it can be used with 100% aqueous mobile phase, also exhibits poor retention
stability after stopping pump. This phenomenon is caused by poor hydration of thosephases. Polar compounds cannot be well-distributed between mobile phase andstationary phase.

YMC Co., Ltd.

http://www.ymc.co.jp

Reproducibility on Triart C181234Retention stability of Adenine

(%)
120

5500 thAdenine

100
80400 th60
40300 th20200 th0Nucleic bases

681012141618200

300

400

500

Column: YMC-Triart C185 µm, 150 X 3.0 mmI.D.
Eluent: 20 mM KH2PO4-K2HPO4 (pH 6.9)
Flow rate: 0.425 mL/minTemperature : 37℃Detection: UV at 254 nm

1. Cytosine
2. Uracil
3. Guanine
4. Thymine
5. Adenine

1 st4100

Number of analysis

100 thJ121101A
02020 minNo change is found in separation characteristics of Triart C18 including retention time even after 500 runs.

Water soluble vitamins
Comparison of three YMC phases that can be used with 100% aqueous
2mAU
80

YMC-Triart C1860

53104

176

40

1198

1213

20U120222A300

2.5mAU80

Hydrosphere C18512.5

15104

140

1052360

7.567817.5

min11912 13

20U120224A30mAU

YMC-Pack ODS-AQ0

2.55

7.5

2380
60

1012.517.5

min9, 1054115640711812 13200

Water soluble vitamins

U120224C30

1. L-Ascorbic acid 2-glucoside
2. L-Ascorbic acid
3. D-Isoascorbic acid
4. Nicotinic acid
5. Nicotinamide
6. D-(+)-Pantothenic acid calcium salt
7. Pyridoxal hydrochloride
8. Pyridoxine hydrochloride
9. Foric acid
10. Thiamine hydrochloride
11. Cyanocobalamin
12. D-Biotin
13. Riboflavin

2.55

7.5

10

12.5

15

17.5

minColumnEluent: 3 µm, 150 X 3.0 mmI.D.
: A) 20 mM KH2PO4-H3PO4 (pH 2.8) containing 5 mM CH3(CH2)5SO3Na
B) 20 mM KH2PO4-H3PO4 (pH 2.8)/acetonitrile (80/20) containing 5 mM CH3(CH2)5SO3Na
0%B (0-3 min), 0-75%B (3-13 min), 75%B (13-20 min)FLow rate: 0.425 mL/min
Temperature : 40℃
Detection: 210 nmRetention behavior of water soluble vitamins on three YMC ODS phases that can beused with 100% aqueous mobile phase is compared. Retention time and elution orderof Folic acid (peak 9), Thiamine hydrochloride (peak 10), and Cyanocobalamin (peak 11)
are diﬀerent among three phases. This is because the balance of hydrophobicity andhydrogen bonding capacity diﬀers from column to column.

YMC Co., Ltd.

http://www.ymc.co.jp

Organic acids
Comparison of three YMC phases that can be used with 100% aqueous
2, 3mAU40

15 6308910

1. Oxalic acid
2. Tartaric acid
3. Glycolic acid
4. Formic acid
5. L-Malic acid
6. Malonic acid
7. Lactic acid8. Acetic acid
9. Maleic acid
10. Citric acid
11. Succinic acid
12. Fumaric acid
13. Acrylic acid14. Propionic acid1311720Organic acids

YMC-Triart C183 µm, 150 x 3.0 mmI.D.

12414100U111212Q5244303208126, 72mAU

61581310minHydrosphere C183 µm, 150 x 3.0 mmI.D.

10 11914Eluent: 20 mM H3PO4: 0.425 mL/min
FLow rate
Temperature : 37℃: 220 nmDetection

100U111207Q42mAU

4682, 34013041256 82010

YMC-Pack ODS-AQ
3 µm, 150 x 3.0 mmI.D.

1310 9 , 117min

14100U111209Q72468min10Retention behavior of organic acids on three YMC ODS phases that can be used with 100% aqueous mobile phase is compared.
Retention time and elution order of Oxalic acid (peak 1) and Maleic acid (peak 9), which have relatively high acidity, are diﬀerent
among three phases. This is attributed to weak anion exchange eﬀect on Hydrosphere C18 and ODS-AQ.

Method transfer between UHPLC and HPLC
YMC-Triart C185 µm, 250 x 3.0 mmI.D.

2, 3mAU35304125125 6201578910131114
P=8 MPa

1050U111220B012.53 µm, 150 x 3.0 mmI.D.

57.5

1012.51517.5mAU20 min

403020P=11 MPa

1001.9 µm, 100 x 3.0 mmI.D.

U111221B012mAU
60

46812 min

1050
40
30
201001mAU

Flow rate: X20.85 mL/min

P=28 MPa

U111216B02

23456750

8 min

4030
20100P=54 MPa

U111221D20.5

11.5

22.5

33.54 minIdentical chromatographic performance including peak shape and selectivity of Triart C18 column across diﬀerent particle sizes
provides mutual method transfer between UHPLC and HPLC. This feature is ideal for ultrafast method development, and following
method transfer to HPLC.

YMC Co., Ltd.

http://www.ymc.co.jp