Preparative HPLC System

Specifications
LC-Forte/R-Ⅱ

Detector (selectable)
LC-Forte/R-Ⅱ

Flow rate range

0.1-80 mL/min

Accuracy of flow rate

±2% (over 5 mL/min)

System pressure limit

30 MPa（0.1-50 mL/min）

Wavelength

10.4 inch(on main body)

OS : Windows Embedded Standard 7 or

YRI-8830

UV

RI

200-400 nm−(3 Variable wavelengths）

20 MPa（50-80 mL/min）
LCD touch screen

YUV-3400

Windows 10 IoT

Source of lightD2 lampLED

Sensitivity/noise

1.3 X 10-4 AU

1.4 X 10-6 RIU

Drift

3.0 X 10-3 AU/h

7.9 X 10-6 RIU/hFlow cell−

8 µL

Saturated concentration−

Over 4 X 10-3 RIUSingle-beam transmissive-typeDeflectionControl

LCD touch screen or interlinked PCStacking injection

Suctioned by pump

Manual injection

By accompanying injector loop (5 mL as standard)

Manual/Auto-mode

Operation for injection, recycle and fraction

Auto-replay

Log-expanding auto separation method

Cleanup

Auto-flushing for flow line, column-flushing

RecycleBy switching of electromagnetic-valve

PC control (*optional)

By dedicated software, with LAN connection

Event marking

Any events (start, inject, fraction, etc.)

Timer for pump

Automatically start/stop at the input time

Fraction method

Replay, manual, peak (controlled by interlinked PC)

Pressure limiter

Available at any setting values

Number of fraction

Max. 65 fractions (standard rack: 21 fractions)

Display for chromatogram

On LCD

Rack size

φ18 X 65, φ40 X 21 (standard), φ105 X 6,

Storage of operation data

7 GB internal memory/flash device or interlinked PC

Port

LAN : 1, USB : 4

Dimension

300 (W) x 500 (D) x 300 (H) mmSensor for leaking

Installed

Power supply

AC100-240 V

Storage of detector

In the enclosure (ALL IN ONE）

Weight15 kg

Column holder

Installed as standard

Dimension

270（W）X 570（D）X 675（H）mm

Power supply

AC100-240 V（400 W）

WeightApprox. 40 kg

Measuring method
RI range−

1.00-1.75

Purge process−

Automatic

Compact & Multifunctional!
Suitable for Purification on Lab-scale!

* The standard YUV-3400 is downgradable to other detectors depending on theintended purpose.

Fraction Collector (optional)
Forte/FR

customizable for other size

Buffer Rack (optional)
Forte/RK
Column holder

Installed as standard

Dimension

400（W）X 500（D）X 420（H）mmWeight8 kgParts

Column holder

Drain port

LCD touch screen

Column port

Column switching valve
Manual injector

Solvent port
Suction injector

Pump

Before use (installation, operation, maintenance or check-up) of our products, instruction manuals should be carefully read and understood, and safety rules and precautions followed
as outlined in the manuals.

YMC America, Inc.

YMC Europe GmbHYMC Switzerland LLC

YMC Shanghai Rep. Officewww.ymcamerica.com

www.ymc.de

www.ymc-schweiz.ch

www.ymcchina.com

YMC India Pvt. Ltd.

YMC Korea Co., Ltd.

YMC Taiwan Co., Ltd.

YMC Singapore Tradelinks Pte. Ltd.

www.ymcindia.com

www.ymckorea.com

www.ymctaiwan.com

www.ymc.co.jp/en/Distributor

YMC Karasuma-Gojo Bldg., 284 Daigo-cho
Karasuma Nishiiru Gojo-dori, Shimogyo-ku
Kyoto, 600-8106, Japan
TEL:+81-75-342-4515 FAX:+81-75-342-4550
www.ymc.co.jp sales@ymc.co.jp
The above information is as of October, 2019 and is subject to change without notice. ©2019 YMC CO., LTD. All Rights Reserved.

http://www.ymc.co.jp
190925A

LF-0041E

Dedicated Software
LC system designed for both preparative HPLC andflash purification
User friendly operation with a touch screen
Intuitive method development by Preview Function
Multiple functions like recycling, auto-injection andauto-replay as a standard feature
Compact design installable in fume hoods

A wide touch screen (10.4 inch) with the pre-installed software offers friendly navigation for settings and operation of thesystem. Chromatograms can be displayed on the screen.

Ap60 pro
0 x.
mm

Approx. 700 mm

FeaturesApprox. 750 mm

For Purification from Several Milligrams to 10 Grams on Lab-scale
LC-Forte/R-Ⅱ is useful for lab-scale preparative purifications with HPLC columns, glass columns and flash chromatographycolumns. It is applicable for various modes such as reversed-phase, normal-phase, chiral and GPC.

Preview Function for Intuitive Method Development
LC-Forte/R-Ⅱ is equipped with Preview Function that displays recycling and fractioning positions in the chromatograms
acquired from the preliminary study. This function allows you to visually set and check the optimal separation conditions.
Thus, it enables efficient and reliable purification by intuitive understanding of the separation methods. It is especially effective
for complex conditions such as recycling HPLC that may cause sample loss due to the fraction collection setting errors.

HPLC Columns

For reversed- and normal-phase, chiral and GPC

Glass Columns

For purification with self-packing columns

Flash Chromatography Columns

TargetFor synthetic reaction products

10 mg

100 mg1g

Fractionation

LC-Forte/R-Ⅱ is equipped with a recycling function. Under recycling chromatographic separation, higher resolution can beachieved on a sample that is hardly separated, because the sample is repeatedly introduced into the column. Desired purity
can be achieved without changing mobile phase conditions or columns. The unique flow path for the recycling system adopted
in LC-Forte/R-Ⅱ enables to reduce the extra-column diffusion and achieves the high efficiency preparative separation.
Furthermore, consumption of solvent on purification is greatly reduced since no solvent is consumed during recycling.
Detector

Injector

Recycle valve

Fraction/Drain

RecycleMobile phase
1st recycling

Poor resolution

Fractionation

Preview Function enables to find and avoid error setting
for fraction collection easily.

Increasing Separation Efficiency by Recycling

PumpTarget

10 gPurification scaleColumnParameterchanges

After the change of method parameters, you can check if
the new fractionation window works.

Stacking Injection
With a suction injector LC-Forte/R-Ⅱ can inject the same sample repeatedly with high precision. Since this makes it possible
to perform the preparative purification continuously, LC-Forte/R-Ⅱ is useful for efficient separation of a large amount ofsample volume.
Separation time and solvent consumption canbe reduced by injecting the samples withoverlapping the elution peaks of the impurities(but not target compounds). Productivity isimproved dramatically by using the stacking
injection.

A-2
A-1

Singleinjection
12

3
B-1

A-1

Stacking
injection

B-2

1

Providing the resolution corresponding to three coupled columns by one column

B-2

A-2

2nd recycling

Good resolution

B-1

23

Dedicated Software
LC system designed for both preparative HPLC andflash purification
User friendly operation with a touch screen
Intuitive method development by Preview Function
Multiple functions like recycling, auto-injection andauto-replay as a standard feature
Compact design installable in fume hoods

A wide touch screen (10.4 inch) with the pre-installed software offers friendly navigation for settings and operation of thesystem. Chromatograms can be displayed on the screen.

Ap60 pro0 x.
mm

Approx. 700 mm

FeaturesApprox. 750 mm

For Purification from Several Milligrams to 10 Grams on Lab-scale
LC-Forte/R-Ⅱ is useful for lab-scale preparative purifications with HPLC columns, glass columns and flash chromatography
columns. It is applicable for various modes such as reversed-phase, normal-phase, chiral and GPC.

Preview Function for Intuitive Method Development
LC-Forte/R-Ⅱ is equipped with Preview Function that displays recycling and fractioning positions in the chromatograms
acquired from the preliminary study. This function allows you to visually set and check the optimal separation conditions.
Thus, it enables efficient and reliable purification by intuitive understanding of the separation methods. It is especially effective
for complex conditions such as recycling HPLC that may cause sample loss due to the fraction collection setting errors.

HPLC Columns

For reversed- and normal-phase, chiral and GPC

Glass Columns

For purification with self-packing columns

Flash Chromatography Columns

TargetFor synthetic reaction products

10 mg100 mg

1g

Fractionation

LC-Forte/R-Ⅱ is equipped with a recycling function. Under recycling chromatographic separation, higher resolution can beachieved on a sample that is hardly separated, because the sample is repeatedly introduced into the column. Desired purity
can be achieved without changing mobile phase conditions or columns. The unique flow path for the recycling system adopted
in LC-Forte/R-Ⅱ enables to reduce the extra-column diffusion and achieves the high efficiency preparative separation.
Furthermore, consumption of solvent on purification is greatly reduced since no solvent is consumed during recycling.
Detector

Injector

Recycle valve

Fraction/DrainRecycle
Mobile phase
1st recycling

Poor resolution

Fractionation

Preview Function enables to find and avoid error setting
for fraction collection easily.

Increasing Separation Efficiency by Recycling

Pump

Target

10 g

Purification scale

ColumnParameterchanges

After the change of method parameters, you can check if
the new fractionation window works.

Stacking Injection
With a suction injector LC-Forte/R-Ⅱ can inject the same sample repeatedly with high precision. Since this makes it possible
to perform the preparative purification continuously, LC-Forte/R-Ⅱ is useful for efficient separation of a large amount ofsample volume.
Separation time and solvent consumption canbe reduced by injecting the samples withoverlapping the elution peaks of the impurities(but not target compounds). Productivity isimproved dramatically by using the stacking
injection.

A-2
A-1

Singleinjection
1

2

3
B-1

A-1

Stacking
injection

B-2

1

Providing the resolution corresponding to three coupled columns by one column

B-2

A-2

2nd recycling

Good resolution

B-1

2

3

Preparative HPLC SystemSpecifications
LC-Forte/R-Ⅱ

Detector (selectable)
LC-Forte/R-Ⅱ

Flow rate range

0.1-80 mL/min

Accuracy of flow rate

±2% (over 5 mL/min)

System pressure limit

30 MPa（0.1-50 mL/min）

Wavelength

10.4 inch(on main body)

OS : Windows Embedded Standard 7 or

YRI-8830

UVRI

200-400 nm−(3 Variable wavelengths）

20 MPa（50-80 mL/min）
LCD touch screen

YUV-3400

Windows 10 IoT

Source of lightD2 lampLED

Sensitivity/noise

1.3 X 10-4 AU

1.4 X 10-6 RIU

Drift

3.0 X 10-3 AU/h

7.9 X 10-6 RIU/hFlow cell−

8 µL

Saturated concentration−Over 4 X 10-3 RIUSingle-beam transmissive-typeDeflection

Control

LCD touch screen or interlinked PC

Stacking injection

Suctioned by pump

Manual injection

By accompanying injector loop (5 mL as standard)

Manual/Auto-mode

Operation for injection, recycle and fraction

Auto-replay

Log-expanding auto separation method

Cleanup

Auto-flushing for flow line, column-flushing

RecycleBy switching of electromagnetic-valve

PC control (*optional)

By dedicated software, with LAN connection

Event marking

Any events (start, inject, fraction, etc.)

Timer for pump

Automatically start/stop at the input time

Fraction method

Replay, manual, peak (controlled by interlinked PC)

Pressure limiter

Available at any setting values

Number of fraction

Max. 65 fractions (standard rack: 21 fractions)

Display for chromatogram

On LCD

Rack size

φ18 X 65, φ40 X 21 (standard), φ105 X 6,

Storage of operation data

7 GB internal memory/flash device or interlinked PC

Port

LAN : 1, USB : 4

Dimension

300 (W) x 500 (D) x 300 (H) mm

Sensor for leaking

Installed

Power supply

AC100-240 V

Storage of detector

In the enclosure (ALL IN ONE）

Weight15 kg

Column holderInstalled as standard

Dimension

270（W）X 570（D）X 675（H）mm

Power supply

AC100-240 V（400 W）

WeightApprox. 40 kg

Measuring method
RI range−

1.00-1.75

Purge process−

Automatic

Compact & Multifunctional!
Suitable for Purification on Lab-scale!

* The standard YUV-3400 is downgradable to other detectors depending on theintended purpose.

Fraction Collector (optional)
Forte/FR

customizable for other size

Buffer Rack (optional)
Forte/RK
Column holder

Installed as standard

Dimension

400（W）X 500（D）X 420（H）mm

Weight8 kg

Parts

Column holder

Drain port

LCD touch screen

Column port

Column switching valve
Manual injector

Solvent port
Suction injector

Pump

Before use (installation, operation, maintenance or check-up) of our products, instruction manuals should be carefully read and understood, and safety rules and precautions followed
as outlined in the manuals.

YMC America, Inc.

YMC Europe GmbHYMC Switzerland LLC

YMC Shanghai Rep. Officewww.ymcamerica.com

www.ymc.de

www.ymc-schweiz.ch

www.ymcchina.com

YMC India Pvt. Ltd.

YMC Korea Co., Ltd.

YMC Taiwan Co., Ltd.

YMC Singapore Tradelinks Pte. Ltd.

www.ymcindia.com

www.ymckorea.com

www.ymctaiwan.com

www.ymc.co.jp/en/Distributor

YMC Karasuma-Gojo Bldg., 284 Daigo-cho
Karasuma Nishiiru Gojo-dori, Shimogyo-ku
Kyoto, 600-8106, Japan
TEL:+81-75-342-4515 FAX:+81-75-342-4550
www.ymc.co.jp sales@ymc.co.jp
The above information is as of October, 2019 and is subject to change without notice. ©2019 YMC CO., LTD. All Rights Reserved.

http://www.ymc.co.jp
190925A

LF-0041E