Comparison of separation selectivity among
YMC-Triart series
Separation of structural isomers by utilizing uniqueseparation characteristics of Triart Phenyl/PFP(F130201AE)

Specifications of Triart series for reversed phase chromatography
Triart C18
Functional group

Triart C8

Triart Phenyl

Si－C8H17Si

Triart PFP
F

Si－C18H37

FSi

FF

Base

organic/inorganic hybrid silica

Particle

5 m, 3 m, 1.9 m

Pore

12 nm

Endcapping

Multi-stage endcapping

pH range

1~12Upper limit oftemperature

70℃ for pH 1~7,50℃ for pH 7~12FNo
1~10

1~850℃

Specifications of other columns
YMC-Pack Ph

YMC-Pack CN

Conventional
Phenyl-Hexyl

ConventionalPFP

Functional groupFSiSiCNSiSiFFBaseSilicaFSilica

SilicaSilica5 m,3 mParticle

5 m, 3 m

5 m, 3 m

5 m,
3.5 m,
1.8 m

Pore

12 nm

12 nm

9.5 nm12 nm

Endcapping

Yes

Yes

Yes

Yes

pH range

2~7.5

2~7.5

2~82~7.5

Upper limit oftemperature

50℃

50℃

60℃

75℃

F

1

Comparison of Separation Selectivity
Among YMC Reversed Phase Columns
mAU
120

Triart C18

- interaction index

80k’(7) 9.95

3140Hydrophobicity index

(4/7) 0.42

2546789P120830N0mAU05120

Triart C8101520(4/7) 0.4880
1404, 56789P120828D0mAU0120

Triart Phenyl

5101580
2k’(7) 3.37

31654

79,8P121206A0mAU

0510min120

3,46 1,72
50895470Hmin

4,7OP120914L

05 min: 5 µm, 150 X 3.0 mmI.D.
: 20 mM KH 2PO 4-H 3PO 4 (pH3.1)/methanol (25/75)

Non-polarAcidicCH3CH3HO94. NaphthaleneH

3. Testosteronek’(7) 0.44

8 1

20

- interaction

CH3 H

(4/7) 0.98

40Neutral
CH3OH

3,56OH

2. 8-Quinolinol

Polar98

580

N

CH315 min

P120914B

120ColumnEluentN(4/7) 0.67

0YMC-Pack CN

CH3P121206Bk’(7) 1.23

40Coordination

1. Amitriptyline

1,280

mAU

10

3, 5, 6120

Basick’(7) 2.230mAU

Phenyl and PFP: interaction and polar interaction as wellas hydrophobic interaction contribute toseparation. Different separation selectivity
from Triart C18/C8.

(4/7) 0.90

8040

YMC-Pack Phmin(4/7) 0.58

40

Triart PFPmin

C8:
Similar selectivity to C18 and shorter
retention times than C18k’(7) 5.53

2 325CH3O

6. Propylbenzene

CH3

5. Ibuprofen

CH3

7. n-Butylbenzene

Planar recognition ability

Flow rate: 0.425 mL/min
Temperature : 40℃
Detection: UV at 265 nm
Injection: 4 µL

8. o-Terphenyl (Steric)9. Triphenylene (Planar)2① Hydrophobicity② Polarity

9.95

Triart C185.53

Triart C8
Triart Phenyl

3.37

Triart PFP

0.24

Triart C80.26
0.44

Triart Phenyl2.23

YMC-Pack Ph

Triart C180.90

Triart PFP
YMC-Pack Ph

1.23

YMC-Pack CN 0.44

0.80

YMC-Pack CN

0510150.74

0.2k‘ (n-Butylbenzene)

1.21

Triart C18

Triart C8

1.13

Triart C8

Triart PFP

0.58
0.90

YMC-Pack Ph

0.93

0.67
0.98

YMC-Pack CN

1.78
1.5

2.5

1.2

0.48

Triart PFP

3.06

YMC-Pack CN

1.00.42

Triart Phenyl

0.98

0.5

0.8

④  interaction

Triart C18

YMC-Pack Ph

0.6

(Testosterone/n-Butylbenzene)③ Planar recognition ability

Triart Phenyl

0.4

0.0

3.5

0.5

1.0(Triphenylene/o-Terphenyl)(Naphthalene/n-Butylbenzene)⑤ Basic compound⑥ Coordination compound

Triart C18

1.17

Triart C18

1.13

Triart C8

1.14

Triart C81.24

Triart Phenyl

1.08

Triart Phenyl

1.10
1.15

Triart PFP

1.17

Triart PFP

YMC-Pack Ph

1.18

YMC-Pack Ph

YMC-Pack CN

1.16

YMC-Pack CN

0.5

1.0

1.5

Tf (Amitriptyline)

2.0

0.0

2.16
4.86
1.0

2.0

3.0

4.0

Tf (8-Quinolinol)

5.0
3Separation Comparison of Terphenyl Positional Isomers
mAU

3, 240

Triart C18
120

80% methanol
0F121228K05101520min

mAU
40

Triart C83

Triart C8 which has higher bonding density
can separate p- and m- terphenyl while
Triart C18 cannot separate them.

2075% methanol
021Rs (1,2) = 4.83
Rs (2,3) = 1.61

F121228D

05101520min

mAU
40

Triart Phenyl

Resolution of aromatic positional isomers
is improved by  interaction of TriartPhenyl.

2075% methanol
0321

Rs (1,2) = 3.17
Rs (2,3) = 1.79

F121228G

05101520min

20min

mAU

2, 340

Triart PFP

12070% methanol0F130107B

051015mAU

340Elution order (peak 2 and 3) is different
compared to Triart Phenyl that has butylgroup as spacer.

YMC-Pack Ph
2065% methanol
021Rs (1,3) = 4.74
Rs (3,2) = 1.32

F130107D

05101520min

mAU
40

3

Conventional

Phenyl-Hexyl

2120

Rs (1,2) = 6.63
Rs (2,3) = 1.63

75% methanol
0F130107G

05101520min

20minmAU

340Conventional

PFP

122070% methanol

0F130108H

051015Terphenyl positional isomers
12ColumnEluent
Flow rate3Temperature
Detection
Injectiono-Terphenyl

p-Terphenyl

m-Terphenyl: 5 µm, 150 X 3.0 or 4.6 mmI.D.
: methanol/water: 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
: 30℃: UV at 254 nm: 2.0 µL for 3.0 mmI.D.
4.7 µL for 4.6 mmI.D.

4

Separation Comparison of Dinitrobenzene Positional Isomers
mAU

2120

1

Triart C18

360

0F121225M

02468

1012141120minRs (1,2) = 4.29

Triart C8

Rs (2,3) = 3.93

360

Triart C8, Phenyl and PFP canseparate m- and o-dinitrobenzene(peak2 2 and4 3) underthe same
68mobile phase condition.

00mAU
120F121226C

1000mAU
120

121416182

Triart Phenyl and PFP giveequivalent or longer retention ofdinitrobenzenes than Triart C18and C8 that are highly hydrophobicphase.
 4 interaction
or 8polar
26interaction between Phenyl andPFP and compounds would
contributetothisretention
behavior.

60

Triart PFP

182mAU

Triart Phenyl

16min

Rs (1,2) = 2.79
Rs (2,3) = 2.11

13F121226B

1012141618min

21

Rs (1,2) = 3.11
Rs (2,3) = 4.62360

0F130108J

0246810121416181012141618min

1, 2mAU

3120

YMC-Pack Ph

600F130108K

02

468min1, 3mAU

2120

Conventional

Phenyl-Hexyl

p- and o-dinitorobenzene are not separated well though
the retention of them is greater compared to Triart Phenyl.

600F130109A

02

46mAU

Conventional

1012

1120

PFP

8141618min

Rs (3,1) = 13.09
Rs (1,2) = 13.80

260

30F130109B

051015202530min

Dinitrobenznene positional isomers
1NO 22ColumnEluent
Flow rate

3NO 2NO 2NO 2NO 2

p-Dinitrobenzene

NO 2m-Dinitrobenzene

o-Dinitrobenzene

Temperature
Detection
Injection: 5 µm, 150 X 3.0 or 4.6 mmI.D.
: methanol/water (40/60): 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
: 30℃: UV at 254 nm: 2.1 µL for 3.0 mmI.D.
5.0 µL for 4.6 mmI.D.

5

Separation Comparison of Brilliant Blue FCF and Its Impurities
1mAU

Triart C1810045% methanol

500A, B

C10B0DEF

64810024681012141618min

CC1mAUTf (1) = 1.28A, B

E10100D
5004624681012141618minCTf (1) = 1.33

150

10B

A

002466810121416

1150

1881012141, C, B

10C, B50

E0406810F130117B

024681012141618min

mAU
150

Tf (1) = 1.70

1F18A

B

Elution order isdifferent fromthat on phenylor alkyl typestationaryphase.

1B

10C500CmAU

10055% methanol

16min

mAU

Tf (1) = －100

YMC-Pack Ph

EDF130116GmAU

45% methanol

B

C5001mAU

1100Triart PFP

8F130117A

045% methanol

F0B

mAU

Triart Phenyl

14115045% methanol

12F130116H

mAU

Triart C8C1mAU

Tf (1) = 1.56

150

DE

01012141618F130118A

024681012141618min

mAU

Tf (1) = 7.73

150

Conventional

Phenyl-Hexyl

Adsorption

100
5045% methanol

B0C

1F130117E

024681012141618min

Phenyl type phases provide preferable
retention and separation on brilliant blue
FCF, acidic triphenylmethane dye, and its
impurities.

mAU
150

Conventional

Tf (1) = 3.72

Adsorption

100PFP

15045% methanol

0F130118F024681012141618min

Brilliant blue FCF
1C 2 H5N

NaO 3 S

A–E
: Impurities in Brilliant blue FCF reagent

C 2H 5N+NaO 3SSO 3-Column
EluentFlow rate
Temperature
Detection
Injection: 5 µm, 150 X 3.0 or 4.6 mmI.D.
: 0.1% phosphoric acid/methanol: 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
: 40℃: UV at 630 nm: 2.0 µL for 3.0 mmI.D.
4.7 µL for 4.6 mmI.D.

Brilliant blue FCF
6Separation Comparison of Piperine cis-trans Isomers
mAU
40

Triart C18

1, 2, 420

30F121214C

0510152025min

25min

mAU
40

Triart C8

1, 2, 420

30F121214G

05101520mAU
40

1, 2, 4

Triart Phenyl

Triart PFP with high planar
recognition ability can separate
piperine cis-trans isomers that ishardly achieved with other reverse2025minphase columns. Various interactions
such as  interaction and dipoledipole interaction would contribute tothe high resolution.

3200F121214D

051015mAU
40Triart PFP

Rs (1,2) = 1.19
Rs (2,3) = 1.54

2001324F121213E

0510152025min

152025min

152025min

152025min

1, 2, 4mAU
40

YMC-Pack Ph

3200F121213D

0510mAU

Conventional

Phenyl-Hexyl

1, 2, 440

3200F121217B

0510mAU1, 240Conventional

PFP

32040F121213C

0510Piperine and its isomers
12HHHO

3O

HOO

HNO

HO

N

H

HHO
HO

ColumnEluent

4HHHOO

HOONH

H

N

Chavicine

Isopiperine

Isochavicine

Piperine

Z,Z-(cis-cis)-piperine

Z,E-(cis-trans)-piperine

E,Z-(trans-cis)-piperine

E,E-(trans-trans)-piperine: 5 µm, 150 X 3.0 or 4.6 mmI.D.
: 0.1% formic acid/acetonitrile(60/40)Flow rate: 0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
Temperature : 25℃
Detection: UV at 280 nm
Injection: 4.25 µL for 3.0 mmI.D.
10.0 µL for 4.6 mmI.D.

7