OligoSep™ Columns for Oligo Prep & Analysis
Access our Best-in-class Chromatography for your Oligo Pipeline!

With the advent of FDA-approved antisense and siRNA therapeutics, and parallel approvals of LNP
technology, your oligonucleotide candidates have a clear path to the clinic. ADS Biotec has quietly
supplied best-in-class analytical chromatography columns — OligoSep, RNASep, and DNASep — toenable critical proof of concept data without unwanted variables or contaminants. Recent “N of 1”
disease opportunities also match well with ADS Biotec’s small-scale preparative chromatography
products.
All ADS columns are supplied with the highest quality buffers on the market, which are used in quality
release prior to delivery. ADS is also honored to have our columns and buffers cited in the recent USP
analytical guidelines for mRNA (https://go.usp.org/mRNAVaccineQuality). ADS also has been audited
for GMP supply to several global CMOs, if scaleup is in your plans.
Following are a series of data figures showcasing the clean baselines and excellent purification
dynamics provided by ADS columns and buffers, available exclusively from ADS Biotec.

Contact us anytime for a confidential review of how our Oligo Column
and Buffers can assist you!
Email info@adsbiotec.com or call 800.402.3200
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Email: info@adsbiotec.com

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Duplex Short Oligo (siRNA) Performance – Either via Denaturing or Non-Denaturing Approaches

Figure 1. Analysis of duplex siRNA purified from 50 OD units of crude, annealed oligonucleotides (100 OD units total). Crudesense and antisense fire-fly luciferase siRNA oligonucleotides were annealed, and duplex siRNAs were purified using theoptimized siRNA purification protocol Purified duplex siRNA was dried, resuspended in water and 1 µg was analyzed on
the WAVE System 3500HT using the OligoSep Cartridge and WAVE Optimized TEAA Buffers under non-denaturing conditions
to evaluate duplex purity, and under fully-denaturing conditions to confirm absence of synthesis failures in purified duplexes.
Refer to the Materials and Methods section in our Application Note 121 Purification of Duplex siRNAs
Directly from Crude, Annealed Oligonucleotides for more information.

ADS Biotec Columns Offer Excellent Discrimination — Including Detection of N-1 Species
A

B

Figure 2. Analysis of fractions collected during separation of annealed Lamin A/C siRNA oligonucleotides under non-denaturingconditions. (A) Lamin A/C siRNA oligonucleotides (10 OD units total, 5 OD units of each crude oligonucleotide) were annealed
in deprotection milieu and separated on the OligoSep Prep HC Cartridge using WAVE Optimized HA Buffers. Individual fractions
were collected at the times shown, dried and resuspended in water. (B) Equal amounts (1 µg) of oligonucleotide from eachfraction were analyzed under fully-denaturing conditions with the WAVE System 3500HT, OligoSep Cartridge and WAVE
Optimized TEAA Buffers.
Refer to the Materials and Methods section in our Application Note 121 Purification of Duplex siRNAs
Directly from Crude, Annealed Oligonucleotides for more information.

Oligonucleotide Separation and Baseline Performance – 20/21mer Discrimination
Column: NUC-99-3550
Sample: Oligonucleotide Standard 20/21mer
Mobile phase: A-0.1 M TEAA in water B; – 0.1 M TEAA in 25% acetonitrile
System: 0.9 ml/min, 80ºC, 5 µL injection

Gradient

LoadingStart Gradient
Stop GradientStart Clean
Stop Clean
Start Equilibrate
Stop Equilibrate

Time05
10.5
10.6
11.1
11.2
12.1

%A9085
700090
90%B
1015301001001010Column: NUC-99-3560
Sample: Oligonucleotide Ladder 20-100 bases
Mobile phase: A-0.1 M HAA in 10% acetonitrile; B – 0.1 M HAA in 50% acetonitrile
System: 0.9 ml/min, 80ºC, 0.9 µg/µL

Gradient

LoadingStart Gradient
Stop GradientStart Clean
Stop Clean
Start Equilibrate
Stop Equilibrate

Time011111.1
12.1
12.2
14.2%A
49
44
200053
53%B
51
56
80
1001004747

Order Information
Catalogue
NumberProduct Name

Product Description

NUC-99-3550

OligoSep™ Column
4.6 mm x 50 mm

The OligoSep™ – HPLC Oligonucleotide analysis column uses
alkylated non-porous polystyrene-divinylbenzene (PS-DVB)
copolymer microspheres for high performance nucleic acidseparations. These versatile columns can be used with a varietyof HPLC systems for oligonucleotide QC and high resolution
ds/ssDNA analysis to 20 µg.

NUC-99-3560

OligoSep™ HC Column
4.6 mm x 50 mm

The OligoSep™ – High Capacity HPLC Oligonucleotide analysis
column uses alkylated macro-porous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres for high performance
nucleic acid separations. These versatile columns can be usedwith a variety of HPLC systems for oligonucleotide purificationor analysis of ds/ssDNA analysis. Typically with loading capacityof approximately 1,700 µg.

NUC-99-3860

OligoSep™ Prep HC Column The OligoSep™ Prep – High Capacity HPLC Oligonucleotide
purification column uses alkylated macro-porous polysty7.8 mm x 50 mm
rene-divinylbenzene (PS-DVB) copolymer microspheres for highperformance nucleic acid separations. These versatile columns
can be used with a variety of HPLC systems for Oligonucleotide
Synthesis Purification, up to 5,000 µg.

NUC-99-3870

OligoPrep™ HC3 Column
7.8 mm x 150 mm

The OligoSep™ Prep – High Capacity HPLC Oligonucleotide
purification column uses alkylated macro-porous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres for highperformance nucleic acid separations. These versatile columns
can be used with a variety of HPLC systems for Purification ofOligo Syntheses where the synthesis exceeds 1 µ mole.

RPC-99-3015

RNASep™ Semi-Prep –Ultra High Capacity RNA
Purification Column
30 mm x 150 mm

The RNASep™ Semi-Prep is an ultra high capacity HPLC RNA
purification column that uses alkylated non-porous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres for highperformance nucleic acid separations. These versatile columns
can be used with a variety of HPLC systems for RNA purification
up to 2 mg. They are also ideal for the development of effectivemRNA vaccines and other novel drug candidates.

RPC-99-2110

RNASep™ Semi-Prep –High Capacity RNA
Purification Column
21.2 mm x 100 mm

RNASep™ Semi-Prep is a high capacity RNA purification columnthat uses alkylated non-porous polystyrene-divinylbenzene(PS-DVB) copolymer microspheres for high performance nucleic
acid separations. These versatile columns can be used with avariety of HPLC systems for RNA analysis and purification. They
are also ideal for the development of effective mRNA vaccinesand other novel drug candidates.

™ 2023 ADS Biotec Inc. All specifications are subject to change without notice.

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