YMC USER'S MANUAL

Column Care and Use Instructions

YMC-Triart, Accura Triart Reversed-Phase Columns
HPLC: 5 µm, 3 µm / UHPLC: 1.9 µm
1. Introduction
Thank you for purchasing a YMC high-performance/ultra-high-performance liquid chromatography (HPLC/UHPLC) column. Triart
is a multipurpose reversed phase column using organic/ inorganic hybrid silica.
YMC-Triart and Accura Triart columns, which are manufactured under highly controlled conditions, must pass a series of strict
tests before being accepted for shipment (Please refer to the column inspection report). To ensure optimal performance anddurability of the column, please read these instructions carefully before using this column.

2. Packing materials Specifications
Packing materialParticle size(µm)

Pore size(nm)

Triart C1812

Triart C18 ExRS

8C18

Triart C8
Triart Phenyl
Triart PFP

UsablepH range

Functional group

Usable temperature range
Regular use(recommended)

Upper limit

1–1220–40°C

pH 1–7 : 90°C
pH 7–12 : 50°C

C8
1.9, 3, 512Phenylbutyl

1–1020–40°C50°C

Pentafluoro phenylpropyl

1–820–40°C50°C

C18

1–1220–40°C

pH 1–9 : 90°C
pH 9–12 : 50°C

C4

1–1020–40°C

pH 1–7 : 90°C
pH 7–10 : 50°C

Triart Bio C1830
Triart Bio C4

3. Column hardware specifications

Column tubing
Column frit

YMC-Triart

Accura Triart

YMC-Triart metal-free PEEK-lined

stainless steel

bioinert coated stainless steel

PEEK-lined stainless steel
PEEK

4. Recommendations for column connections
The "PT", “PTH”, "PTC", "PTP", or "WT" at the end of the product code indicates the style of column endfittings (see below fordetails).

Consideration of connector and endfittings
Tubing and connector

ColumnThe end ofthe product code

Port depth

Style of endfittings

PT/PTH/PTC/PTP

ca. 2 mm / 0.09 inch

Parker style

WTca. 3 mm / 0.13 inch

Waters style

Port depth

Wetted part (Inner surface of column tube) of “PTP” type column is PEEK. Its external tube is made of stainless steel. A special
care has to be taken to connect this “PTP” type column to your system. Refer to the “Instructions for column connection
YMC-Triart metal-free PEEK-lined”. https://www.ymc.co.jp/data/download/metal_free_column_connection_e.pdf

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM230912AE) 1/3

YMC USER'S MANUALTubing must have flat ends and must bottom out in the column endfitting. Tubing must be connected to the column correctly to
avoid creating a void between the column frit and tubing, which can cause a leak and result in poor column performance (e.g.
peak tailing, loss of theoretical plate number).
The 1.9 µm column for UHPLC gives high operating pressure compared to conventional 5 µm/3 µm columns. Particularattention should be devoted to the usable maximum pressure of tubing and LC system. Generally, a UHPLC system that has
60 MPa (8,700 psi) or higher pressure tolerance is recommended for use with a 1.9 µm column. We have a connector with are-adjustable ferrule, which provides flawless sealing at 137 MPa (20,000 psi). Please inquire for related details.

5. Shipping solvent
100% Acetonitrile. Replace with this solvent for storage. When replacing a mobile phase containing buffer salts/additives, extra
care must be taken to prevent salt precipitation.

6. Precautions for use
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ Do not disconnect a column from the LC system before the pressure drops to zero.
・ The column pressure limit and recommended flow rate are the following.
Particle size

1.9 µm

3 µm/5 µmColumn I.D.

Recommended flow rate
(Mobile phase condition: acetonitrile/aqueous solution)

Pressure limit

1.0 mm

0.05–0.2 mL/min

2.0/2.1 mm

0.2–0.8 mL/min

3.0 mm

0.4–1.6 mL/min

2.0/2.1 mm

0.2 mL/min

3.0 mm

0.4 mL/min

4.0 mm

0.7–0.8 mL/min

4.6 mm

1.0 mL/min

6.0 mm

1.7 mL/min

Column length of 150 mm or less : 20 MPa
Column length of 250 mm: 25 MPa

10 mm

4.7 mL/min

10 MPa100 MPa

45 MPa*1 Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
*2 Adjust the flow rate appropriately because the pressure changes depending on the column length, temperature, types of organic solvent etc.

・ Aqueous or non-aqueous solvents can be used as a mobile phase. Repetitive replacement among solvents with large
difference in polarities might degrade the column performance. In general, acetonitrile, methanol, and tetrahydrofuran (THF) arerecommended for regular use (Please also see the recommendation above under alkaline mobile phase conditions). Whenusing THF as a mobile phase, be mindful of the solvent resistance of your system or tubing (PEEK parts are especially
unsuitable for use with THF).
・ Recommendations of pH and temperature for column use are shown in the Packing materials specifications table in section 2.
In addition, please note the following.
 Column lifetime varies depending on conditions of use such as pH, temperature, and mobile phase composition. In general,
usage at higher temperatures, higher concentrations of buffer salts/additives, and lower concentrations of organic solvents
can shorten the column lifetime.
 When using the column under alkaline conditions for a long time, a column should be used with a low concentration (about1–10 mM) of organic buffer, such as triethylamine or glycine, at a lower temperature (less than 30°C). The mobile phase
containing methanol is expected to give a longer column lifetime under alkaline conditions than other organic solvents will.
・ Make sure of the miscibility among the organic solvents and take care to prevent the precipitation of buffer salts/additives toavoid overpressuring the column.
・ When possible, the sample should be dissolved in a solvent that is of the same composition as the initial mobile phase. Using astronger solvent than the initial mobile phase for sample dissolution might result in distorted peak symmetry and degraded
resolution.
・ To prevent exposure of the column to excessive pressure, the mobile phase and sample solution should be filtered through a

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM230912AE) 2/3

YMC USER'S MANUAL
0.2 µm membrane or smaller to remove particulates.
・ When using Triart C18 ExRS column, its extremely high hydrophobicity might cause difficulties of replacement or equilibration
with mobile phase containing low concentration of organic solvent. The mobile phase should be contained more than 15% of
methanol or 10% of other lower polar organic solvent. When replacing methanol aqueous solution with acetonitrile aqueoussolution, mobile phase containing less than 20% of acetonitrile may result in irregularities in retention time or peak shapes. In
these cases, first replace with 60% acetonitrile aqueous solution, and then replace with the mobile phase.
・ The extra column volume has a great impact on band spreading. To minimize the influence of band spreading on
chromatographic performance, especially when using columns in 2.0 mm I.D. and smaller, the LC system should be optimized
as described below.
 The shortest possible length of tubing with narrow inner diameters (tubing less than 0.15 mm, 0.006 inch I.D. isrecommended) should be used for the connection from the injector to the column and from the column to the detector.
Make sure not to have a void in the connection.
 Use a detector equipped with low-volume flow cell designed for the narrow bore column.
 Use an injector for the narrow bore column and a low-volume sample loop.
・ A sampling rate and a detector response (time constant) should be optimized to acquire more than 10 data points across a peak.
For UHPLC analysis using 1.9 µm columns, we recommend a sampling rate of about 10 points per second or higher and a
detector response of 0.1 s or faster to detect the earliest eluting sharp peak properly.

7. Column cleaning (general method)[After using mobile phase not containing buffer salts/additives]
・ Flush the column with solution containing a higher ratio of organic solvent for washing out the compounds that have a great
capacity for retention in the column.
・ Usable concentration of organic solvent is up to 100%. A cleaning solution containing THF might be effective when removing
highly hydrophobic (lipid-soluble) substances that are adsorbed onto the gel.
[After using mobile phase containing buffer salts/additives]・ First replace with a water/organic solution containing no buffer salts or additives (A ratio of water to organic solvent should be set
at the same proportions as a mobile phase). Then flush the column in accordance with the method described above.
[General proposals]・ Flushing with 100% water after using the column around the pH limit might shorten column lifetime. Flush the column withwater/organic solution as described above.
・ Once macromolecules such as proteins or polysaccharides are adsorbed onto the gel, they are hardly removed, even ifsolvents with high eluting capability are used. To avoid contamination of the column by them, conduct sample pretreatment
carefully before introduction into the column. Alternatively, use a guard column.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM230912AE) 3/3