CURRICULUM VITAEIDENTICAL INFORMATION
Name: Hisayoshi Hayashi, Ph D
E-mail: hayashih@u-shizuoka-ken.ac.jp
EDUCATION
April/1987-March/1991 School of Food and Nutritional Sciences,
University of Shizuoka
April/1995-March/1998
Graduate School of Nutritional and Environmental
Sciences, University of Shizuoka
Awarded the degree of PhD in food and nutritional
sciences for a thesis entitled "Cellular mechanisms ofsmall intestinal nutrient transport". Work supervised byProf. Yuichi Suzuki.
EMPLOYMENT
June/1991-May/1995

Research Assistant Professor, Laboratory ofPhysiology,School of Food and Nutritional Sciences,
University of Shizuoka (1991-1993 Prof. TakeshiHoshi,1993-1995 Prof. Yuichi Suzuki)

April/1998-present

Research Assistant Professor, Laboratory ofPhysiology School of Food and, Nutritional Sciences,
University of Shizuoka, (Prof. Yuichi Suzuki)

June/1999-March/2002

Research fellow, Cell Biology Program,
The Hospital for Sick Children, Canada,(Prof. Sergio Grinstein)

April/2002- Sept/2010

Research Assistant Professor, Laboratory of Physiology
School of Food and, Nutritional Sciences,
University of Shizuoka, (Prof. Yuichi Suzuki)

Oct/2010- March/2012

Assistant Professor, Laboratory of Physiology
School of Food and, Nutritional Sciences,
University of Shizuoka

April/2012- PresentAssociate Professor, Laboratory of Physiology
School of Food and, Nutritional Sciences,
University of Shizuoka

MEMBERSHIPSThe Japanese Society of Physiology
The Japanese Biochemical Society
The Japanese Society of Digestion and Absorption
The American Physiological Society
PUBLICATIONS
1. Nakamura C, Ishizuka N, Yokoyama K, Yazaki Y, Tatsumi F, Ikumi N, Hempstock W, Ikari
A, Yoshino Y and Hayashi H; Regulatory mechanisms of glucose absorption in the mouseproximal small intestine during fasting and feeding, Scientific Reports 13, 10838, 2023
2.

Hempstock W, Nagata N, Ishizuka N and Hayashi H;The effect of claudin-15 deletion on
cationic selectivity and transport in paracellular pathways of the cecum and large intestine,
Scientific Reports 13, 6799 ,2023

3.

Ishizuka N, Nagahashi M, Mochida Y, Hempstock W, Nagata N and Hayashi H; Na+dependent intestinal glucose absorption mechanisms and its luminal Na+ homeostasis
across metamorphosis from tadpoles to frogs, American Journal of Physiology, 324, R645R655, 2023

4.

Tanifuji K, Shiozaki Y, Koike M, Uga M, Komiya A, Miura M, Higashi A, Shimohata T,Takahashi A, Ishizuka N, Hayashi H, Ichida Y, Ohtomo S, Horiba N, Miyamoto K andSegawa H; Effects of EOS789, a novel pan-phosphate transporter inhibitor, on phosphatemetabolism : Comparison with a conventional phosphate binder. The Journal of Medical
Investigation, 70, 260-270, 2023

5.

Okamoto E, Matsuda S, Yoshino Y, Morikawa Y, Suenami K, Tabuchi Y, Matsunaga T,Hayashi H and Ikari A; Increase in Paracellular Leakage of Amino Acids Mediated byAging-Induced Reduction of Claudin-4 Expression, The Journal of Nutrition, 153, 33603372, 2023

6.

Furuse M, Nakatsu D, Hempstock W, Sugioka S, Ishizuka N, Furuse K, Sugawara T,Fukazawa Y and Hayashi H, Reconstitution of functional tight junctions with individual
claudin subtypes in epithelial cells. Cell Structure and Function, 48, 1-17, 2023

7.

Hempstock W, IshizukaN, HayashiH : Functional Assessment of Intestinal Tight Junction
Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique. J Vis Exp.,
171. 2021

8.

Hayashi H, Nagai H, Ohba K, Soleimani M and Suzuki Y:Segmental differences in
Slc26a3‑dependent Cl− absorption and HCO3− secretion in the mouse large intestine invitro in Ussing chamber., J Physiol. Sci., 71:5, 2021

9.

Hirota C, Takashina Y, Ikumi N, Ishizuka N, Hayashi H, Tabuchi Y, Yoshino Y,Matsunaga T, and Ikari A :Inverse regulation of claudin-2 and -7 expression by p53 andhepatocyte nuclear factor 4α in colonic MCE301 cells, TISSUE BARRIERS, 9, 1860409,
2021

10. Hempstock W, Sugioka S, Ishizuka N, Sugawara T, Furuse M, Hayashi H: Angulin2/ILDR1, a tricellular tight junction protein, does not affect water transport in the mouselarge intestine. Scientific Reports, 10, 10374 – 10374, 2020
11. Takashina Y, Ishizuka N, Ikumi N, Hayashi H, Manabe A, Hirota C, Tabuchi Y, Matsunaga
T, Ikari A: Upregulation of Claudin-7 Expression by Angiotensin II in Colonic Epithelial
Cells of Mice Fed with NaCl-Depleted Diets. Int J Mol Sci,. 21,1442 , 2020
12. Nakayama M, Ishizuka N, Hempstock W, Ikari A, Hayashi H.: Na+-Coupled Nutrient
Cotransport Induced Luminal Negative Potential and Claudin-15 Play an Important Role in
Paracellular Na+ Recycling in Mouse Small Intestine. Int J Mol Sci, 21, 376 , 2020
13. Ishizuka N, Hempstock W, Hisayoshi Hayashi H.: The Mode of Action of NHE3 Inhibitors
in Intestinal Na+ Absorption. Gastroenterology Medicine & Research 4, 297 - 301 , 2019
14. Ishizuka N, Nakayama M, Watanabe M, Tajima H, Yamauchi Y, Ikari A, Hayashi H.:
Luminal Na+ homeostasis has an important role in intestinal peptide absorption in vivo. AmJ Physiol, 296, G799-809 , 2018

15. Furukawa C, Ishizuka N, Hayashi H, Fujii N, Manabe A, Tabuchi Y, Matsunaga T, Endo S,Ikari A: Up-regulation of claudin-2 expression by aldosterone in colonic epithelial cells ofmice fed with NaCl-depleted diets. Sci Rep., 22, 12223 , 2017
16. Ikari A, Fujii N, Hahakabe S, Hayashi H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga
T, Sugatani J: Hyperosmolarity-Induced Down-Regulation of Claudin-2 Mediated byDecrease in PKCβ-Dependent GATA-2 in MDCK Cells. J Cell Physiol. 230, 2776-87,2015
17. Khan MR, Islam MT, Yazawa T, Hayashi H, Suzuki Y, Uwada J, Anisuzzaman AS,Taniguchi T: Muscarinic cholinoceptor-mediated activation of JNK negatively regulates
intestinal secretion in mice. J Pharmacol Sci. 127, 150-3, 2015
18. Ikari A., Tonegawa C., Sanada A., Kimura T., Sakai H., Hayashi H., Hasegawa H.,
Yamaguchi M., Yamazaki Y., Endo S., Matsunaga T., Sugatani J.: Tight junctional
localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells. J BiolChem., 289, 13112-13123, 2014
19. Khan RI, Yazawa T., Anisuzzaman AS, Semba S., Ma Y., Uwada J., Hayashi H., Suzuki
Y., Ikeuchi H., Uchino M., Maemoto A., Muramatsu I., Taniguchi T.: Activation of focal
adhesion kinase via M1 muscarinic acetylcholine receptor is required in restitution ofintestinal barrier function after epithelial injury. Biochim Biophys Acta., 1842, 635-645,
2014
20. Ikehara O, Hayashi H, Waguri T, Kaji I, Karaki S, Kuwahara A, Suzuki Y.: Luminal
trypsin induces enteric nerve-mediated anion secretion in the mouse cecum. J Physiol Sci.
64, 119-128, 2014
21. Ikari A, Atomi K, Yamazaki Y, Sakai H, Hayashi H, Yamaguchi M, Sugatani J.
Hyperosmolarity-induced up-regulation of claudin-4 mediated by NADPH oxidasedependent H2O2 production and Sp1/c-Jun cooperation. Biochim Biophys Acta. 1833,2617-27, 2013
22. Khan MRS, Anisuzzaman ASM , Semba S, Ma Y, Uwada J, Hayashi H, Suzuki Y, TakanoT, Ikeuchi H, Uchino M, Maemoto A, Ushikubi F, Muramatsu I, Taniguchi T. M1 is amajor subtype of muscarinic acetylcholine receptors on mouse colonic epithelial cells. J
Gastroenterology 48, 885-96, 2013
23. Inoue H, Kobayashi-Yamakawa K, Suzuki Y, Nakano T, Hayashi H, Kuwano T. A casestudy on the association of variation of bitter-taste receptor gene TAS2R38 with the height,
weight and energy intake in Japanese female college students. J Nutr Sci Vitaminol. 59 1621, 2013
24. Hayashi H, Tamura A, Krishnan D, Tsukita S, Suzuki Y, Kocinsky HS, Aronson PS,Orlowski J, Grinstein S, 9 and Alexander RT. Ezrin is Required for the Functional
Regulation of the Epithelial Sodium Proton Exchanger, NHE3. PLoS One 8, e55623, 2013
25. Ikehara O, Hayashi H, Waguri T, Kaji I, Karaki SI, Kuwahara A, Suzuki Y. Subepithelial
trypsin induces enteric nerve-mediated anion secretion by activating proteinase-activatedreceptor 1 in the mouse cecum. J Physiol Sci. 62.211-219, 2012
26. Ikari A, Atomi K, Takiguchi A, Yamazaki Y, Hayashi H, Hirakawa J, Sugatani J
Enhancement of cell-cell contact by claudin-4 in renal epithelial Madin-Darby canine
kidney cells. J Cell Biochem.113, 499-507, 2012

27. Hayashi H, Yamashita Y. Role of N-glycosylation in cell surface expression and protection
against proteolysis of the intestinal anion exchanger SLC26A3. Am J Physiol Cell
Physiol.302, C781-95, 2012
28. Tamura A, Hayashi H, Imasato M, Yamazaki Y, Hagiwara A, Wada M, Noda T, Watanabe
M, Suzuki Y, Tsukita S. Loss of Claudin-15, but Not Claudin-2, Causes Na+ Deficiency
and Glucose Malabsorption in Mouse Small Intestine. Gastroenterology, 140, 913-923,
2011
29. Koivusalo M, Welch C, Hayashi H, Scott CC, Kim M, Alexander T, Touret N, Hahn KM,Grinstein S. Amiloride inhibits macropinocytosis by lowering submembranous pH andpreventing Rac1 and Cdc42 signaling. J Cell Biol. 188, 547-563, 2010
30. Ikehara O, Hayashi H, Watanabe Y, Yamamoto H, Mochizuki T, Hoshino M, Suzuki Y.
Proteinase-activated receptors-1 and 2 induce electrogenic Cl- secretion in the mouse
cecum by distinct mechanisms. Am J Physiol. 299, G115-125, 2010
31. Hayashi H, Suruga K, Yamashita Y.Regulation of intestinal Cl-/HCO3- exchanger
SLC26A3 by intracellular pH. Am J Physiol, 296, C1279-90, 2009
32. Inagaki-Tachibana E, Tsukahara T, Kaji K, Eguchi R, Kanazawa H, Hayashi H, Suzuki Y.
Involvement of DNA fragmentation of enterocytes in mucosal injury to a mouse jejunum
incubated in ussing chambers. Nagoya J Med Sci, 71, 11-18, 2009.
33. Inagaki-Tachibana E, Hayashi H, Suzuki Y.The electrophysiological properties of themucosal barrier in the injured mouse jejunum in Ussing chambers. J Nutr Sci Vitaminol, 54,269-71 2008.
34. Hayashi H, Aharonovitz O, Alexander RT, Touret N, Furuya W, Orlowski J, Grinstein S.
Na+/H+ exchange and pH regulation in the control of neutrophil chemokinesis andchemotaxis. Am J Physiol. 294, C526-34, 2008.
35. Karaki S, Tazoe H, Hayashi H, Kashiwabara H, Tooyama K, Suzuki Y, Kuwahara A.
Expression of the short-chain fatty acid receptor, GPR43, in the human colon. J Mol Histol,39, 135-42, 2008.
36. Inagaki-Tachibana E, Natori Y, Hayashi H, Suzuki Y. In vitro diffusion barriers of themouse jejunum in ussing chambers. J Nutr Sci Vitaminol (Tokyo). 54, 30-8, 2008.
37. Tamura A, Kitano Y, Hata M, Katsuno T, Moriwaki K, Sasaki H, Hayashi H, Suzuki Y,Noda T, Furuse M, Tsukita S, Tsukita S. Megaintestine in claudin-15-deficient mice.
Gastroenterology, 134, 523-34, 2008.
38. Uchiyama H, Hayashi H, Tanji K, Sugimoto O, Suzuki Y. pH stat studies on bicarbonate
secretion in the isolated mouse ileum. Biomed Res. 28, 239-46, 2007.
39. Kawamata K, Hayashi H, Suzuki Y. Propionate absorption associated with bicarbonate
secretion in vitro in the mouse cecum. Pflugers Arch, 454, 253-62, 2007.
40. Kawamata K, Hayashi H, Suzuki Y. Chloride-dependent bicarbonate secretion in themouse large intestine. Biomed Res, 27, 15-21, 2006.
41. Uchiyama H, Hayashi H, Suzuki Y. Functional characterization of Cl-/HCO3- exchange invillous cells of the mouse ileum. Biomed Res. 27, 265-74, 2006.

42. Karaki S, Mitsui R, Hayashi H, Kato I, Sugiya H, Iwanaga T, Furness JB, Kuwahara A.
Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal
mast cells in rat intestine. Cell Tissue Res, 324, 353-60, 2006.
43. Ikari A, Matsumoto S, Harada H, Takagi K, Hayashi H, Suzuki Y, Degawa M, Miwa M.
Phosphorylation of paracellin-1 at Ser217 by protein kinase A is essential for localizationin tight junctions. J Cell Sci, 119, 1781-9, 2006.
44. Inagaki E, Natori Y, Ohgishi Y, Hayashi H, Suzuki Y. Segmental difference of mucosal
damage along the length of a mouse small intestine in an Ussing chamber. J Nutr SciVitaminol (Tokyo), 51, 406-12, 2005.
45. Tamura A, Kikuchi S, Hata M, Katsuno T, Matsui T, Hayashi H, Suzuki Y, Noda T,Tsukita S, Tsukita S. Achlorhydria by ezrin knockdown: defects in theformation/expansion of apical canaliculi in gastric parietal cells. J Cell Biol, 169, 21-8,2005.
46. Ikari A, Hirai N, Shiroma M, Harada H, Sakai H, Hayashi H, Suzuki Y, Degawa M,Takagi K. Association of paracellin-1 with ZO-1 augments the reabsorption of divalentcations in renal epithelial cells. J Biol Chem, 279, 54826-32, 2004.
47. Hayashi H, Szászi K, Coady-Osberg N, Furuya W, Bretscher AP, Orlowski J, Grinstein S.
Inhibition and redistribution of NHE3, the apical Na+/H+ exchanger, by Clostridium
difficile toxin B. J Gen Physiol, 123, 491-504, 2004.
48. Hayashi H, Suzuki T, Yamamoto T, Suzuki Y. Cholinergic inhibition of electrogenic
sodium absorption in the guinea pig distal colon. Am J Physiol, 284, G617-628, 2003.
49. Hayashi H, Szászi K, Coady-Osberg N, Orlowski J, Kinsella JL, Grinstein S. A slow pHdependent conformational transition underlies a novel mode of activation of the epithelial
Na+/H+ exchanger-3 isoform. J Biol Chem, 277, 11090-11096, 2002.
50. Wang C, Hayashi H, Harrison R, Chiu B, Chan JR, Ostergaard HL, Inman RD, Jongstra J,
Cybulsky MI, Jongstra-Bilen J. Modulation of Mac-1 (CD11b/CD18)-mediated adhesionby the leukocyte-specific protein 1 is key to its role in neutrophil polarization andchemotaxis. J Immunol, 169, 415-23, 2002.
51. Tsuchiya Y, Hayashi H, Suzuki Y. Na+-dependent recovery of intracellular pH from acidloading in mouse colonic crypt cells. Tohoku J Exp Med, 193, 1-11, 2001.
52. Sato Y, Hanai H, Nogaki A, Hirasawa K, Kaneko E, Hayashi H, Suzuki Y. Role of thevasopressin V-1 receptor in regulating the epithelial functions of the guinea pig distalcolon. Am J Physiol, 277, G819-828, 1999.
53. Koyama K, Sasaki I, Naito H, Funayama Y, Fukushima K, Unno M, Matsuno S, Hayashi
H, Suzuki Y. Induction of epithelial Na+ channel in rat ileum after proctocolectomy. Am J
Physiol, 276, G975-984, 1999.
54. Hayashi H, Suzuki Y. Regulation of intracellular pH during H+-coupled oligopeptide
absorption in enterocytes from guinea-pig ileum. J Physiol. 511, 573-586, 1998.
55. Kitaoka S, Hayashi H, Yokogoshi H, Suzuki Y. Transmural potential changes associatedwith the in vitro absorption of theanine in the guinea pig intestine. Biosci Biotechnol
Biochem, 60, 1768-1771, 1996.
56. Ikuma M, Hanai H, Kaneko E, Hayashi H, Hoshi T. Effects of aging on the regulation of
intracellular pH in the rat jejunum. J Gerontol A Biol Sci Med Sci, 51, B346-353, 1996.

57. Ikuma M, Hanai H, Kaneko E, Hayashi H, Hoshi T. Effects of aging on the microclimate
pH of the rat jejunum. Biochim Biophys Acta, 1280, 19-26, 1996.
58. Dougen M, Hayashi H, Yajima T, Suzuki Y : Stimulation of bicarbonate secretion byluminal short-chain fatty acid in the rat and human colon in vitro, Jpn J Physiol, 44, 519531, 1994.
59. Hayashi H, Hoshi T. Properties of active magnesium flux across the small intestine of theguinea pig. Jpn J Physiol, 42, 561-75, 1992.
Review
1. Hayashi H, Szászi K, Grinstein S. Multiple modes of regulation of Na+/H+ exchangers.
Ann N Y Acad Sci. 2002, 976:248-58.