Ion S5™/PGM™

Run PCR with a
LymphoTrack Dx
Assay

Purify amplicons

Quantify& prepare
library

PrepareDNAsamples

Unparalleledworkflow simplicity
LymphoTrack Dx Assays put lg/ TCR
next-generation sequencing at your fingertips.

Load & run
Ion S5TM/PGMTM

Analyze

Process datawith included
bioinformatics

These are in vitro diagnostic products, and are not available for sale or use within North America.

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Invivoscribe, Inc.
Tel +1 858.224.6600 | Fax +1 858.224.6601
sales@invivoscribe.com
10222 Barnes Canyon Rd., Bldg. 1
San Diego, CA 92121 | USA

Invivoscribe Technologies, SARL
Tel +33 (0)4.42.01.78.10 | Fax +33 (0)4.88.56.22.89
sales-eu@invivoscribe.com
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Ion S5™/PGM™

Workflow Summary	Using gloved hands, remove the Master Mixes
01
from the freezer. Allow the tubes to thaw;

07	Purify the PCR products using the Agencourt® AMPure® XPPCR Purification system. Add 90 µL of particles to each
50 µL reaction; elute DNA in 40 µL TE buffer.

then gently vortex to mix.	In a containment hood or dead air box, pipette 45 µL
02
of Master Mix into individual wells of a PCR plate(use a different indexed Master Mix for each sampleand control).

08	
Quantify amplicons with an appropriate method(e.g. 2100 Bioanalyzer® or LabChip® GX).

09	Add 0.2 µL Taq DNA polymerase to each Master Mix.
03	Add 5 µL of DNA (at a minimum concentration of04
10 ng/µL) from the unknown and 5 µL of control	Create the library by combining an equal amount of eachamplicon in a single tube (do not include the no templatecontrol).	Add 5 µL of molecular biology grade water to the05
well containing the respective Master Mix for the	Prepare templates using the OT2 or Ion Chef system.

samples to wells containing the respective Master Mix
reactions, then pipette up and down 5-10 times to mix.

no template control, then pipette up and down 5-10times to mix.	Seal the plate and amplify target DNA using the06
following thermal cycler program:Temperature

1TimeCycle95 oC

7 minutes

1

2

95 oC

45 seconds

360 oC

45 seconds

4o72 C

90 seconds

572 oC

10 minutes

1615 C∞

1o

11	Initialize the S5 or PGM. Load S5 [Ion 520, Ion 530] or PGM
12 chip [316 v2 BC, 318 v2 BC].

1314 Start the Ion S5 or Ion PGM run.	Analyze and visualize the acquired data using the15 LymphoTrack Dx Software for the Ion S5/PGM.	Create a Planned Run using the Torrent Browser.

Standardized Program
Step

10 Dilute the library.

29x

NOTE: Always follow the applicable instructions for use (IFU) when
testing samples.

Ordering Information
CATALOG #PRODUCTS

QUANTITY

9-121-0057

LymphoTrack® Dx IGH FR1/2/3 Assay - S5TM/PGM™

12 + 12 + 12 indices - 5 sequencing reactions each

9-121-0007

LymphoTrack® Dx IGH FR1 Assay - S5TM/PGM™

12 indices - 5 sequencing reactions each

9-121-0037

LymphoTrack Dx IGH FR2 Assay - S5 /PGM™

12 indices - 5 sequencing reactions each

9-121-0047

LymphoTrack Dx IGH FR3 Assay - S5 /PGM™

12 indices - 5 sequencing reactions each

9-122-0007

LymphoTrack® Dx IGK Assay - S5TM/PGM™

12 indices - 5 sequencing reactions each

9-227-0007

LymphoTrack® Dx TRG Assay - S5TM/PGM™

12 indices - 5 sequencing reactions each

9-500-0007

LymphoTrack Dx Software - S5 /PGM™

1 CD complimentary with purchase

®

TM®

®

TM

TM

Storage Conditions: -85 °C to -65 °C (DNA controls may be separated from kits and stored at 2 °C to 8 °C).
These are in vitro diagnostic products, and are not available for sale or use within North America.
©2021 Invivoscribe, Inc. (Invivoscribe®). All rights reserved. The trademarks mentioned herein are the property of Invivoscribe and/or its affiliates, or (as to the trademarksof others used herein) their respective owners. Ion S5TM and Ion PGM™, HI-Q™, ONETOUCH™, ION CHEFTM, ION 316TM, ION 318TM, ION 510TM, ION 520TM and ION 530TM aretrademarks of Thermo Fisher Scientific or its subsidiaries. AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies, Inc. AGENCOURT® is a registeredtrademark of Beckman Coulter, Inc. LABCHIP® is a registered trademark of Perkin Elmer.

M-0044 Rev 01 | May 2021