2015/12/15

Biomaterial Science（2015 Schedule）

EG60411

Biomaterial Science
Toshiharu Enomae

Professor, PhD, Paper Device and Eco-friendly materials

2G103, 10:10-11:25, Tuesday#DateContent

1

10/6

History of papermaking

210/13Pulps – Beating and fiber properties

3

10/20

Pulps – Additives and functions

410/27

Papermaking processes & interfiber bonding

511/10Paper– Structural properties

611/17

Paper– Surface properties

711/24Paper–Wetting and absorption properties

812/1

Paper– Mechanical and optical properties

912/8

Polysaccharide chemistry by Assoc Prof Akiko Nakagawa

1012/15

Recent research of paper science and technology

1112/22

Examination

Lecture information and contact
Paper-based sensors to detect
Cu2+ in drinking water Homepage of “Biomaterial Science (T.

Enomae)” http://www.enomae.com/Dye→ Handouts in lecture(講義資料)

Dye + 2 ppm Cu2+
Filter paperwith quinone
derivative dye E-mail address→ t@enomae.com
for any questions and visit to laboratory(Bio-Agr. Bldg. 生農C209 or 総合A618)

Cu2+ 2 ppm （maximum amount allowed by WHO）
Light scattering by paper enhances color difference.

Paper-based power generator
Fluorescence Intensity (a.u.)

Dye

Dye + 2 ppm Cu2+

3000

Paper-based water quality sensor

2500
2000
1500
1000
5000500

520

540560

Wavelength (nm)

Biomaterial Science by Toshiharu Enomae

580

600Blank
5 μM
10 μM
15 μM
20 μM
25 μM
30 μM
35 μM
40 μM
45 μM
50 μM

Paper-based bioassay

Oct 31, 2015

IPPC2015 @ Tokyo1

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Concept
Bacterial culture system using paper
substrate and ink jet printing

Human-operated system

Automatic system

Bacterial cells are dispensed
on media in paper containerusing inkjet printer

Conditions of bacterialgrowth on paper can beobserved by microscope

Tithimanan Srimongkon
Toshiharu Enomae

Oct 31, 2015

IPPC2015 @ Tokyo

48 hours after printing

Agar sheet medium

Agar sheet medium

Dot of E. coli colony

Preparation of paper substrates

Multi cartridge system printerCalcium chloride

Soaking paper in polystyrene(PS) solution for 1 hour anddrying
Toluene (with blue dyedissolved) printed byusing inkjet printerHydrophilic/hydrophobic
paper

Test of hydrophobicity
K. Abe, and et., Anal. Chem. 80 (2008): 6928-6934
Oct 31, 2015

IPPC2015 @ Tokyo

Biomaterial Science by Toshiharu Enomae

Oct 31, 2015

IPPC2015 @ Tokyo

Sodium alginate

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Amount of solution = %CMYK

Bacterial culture

Cyan = CaCl2 aq
Magenta = Yeast extract
Yellow = Bacto trypton
Black = sodium alginate
10 mGel that formed onhydrophilic areas

Oct 31, 2015

IPPC2015 @ Tokyo

Colonies of bacteria growing
on medium after 24 h

Oct 31, 2015

IPPC2015 @ Tokyo

Oct 31, 2015

IPPC2015 @ Tokyo

Conclusion
Hydrogel medium was successfully
formed by reaction on semihydrophilic areas of filter paper withmulti-cartridge ink-jet printer. The
growth of bacteria was successfully
observed with optical microscope.

Oct 31, 2015

IPPC2015 @ Tokyo

Influence of saltwater immersion onproperties of flood-damaged paper
Tunchira Bunyaphiphat
Akiko Nakagawa-Izumi
Toshiharu Enomae

Mold grows and• Characters cannot beread due to color• Bad smell• Health hazard
Flood-damaged paper and books • Artistic value lost
To inhibit mold growth
WashingDryingRestoration… Speedy action is difficult!

Immersion in saltwater

Biomaterial Science by Toshiharu Enomae

was proposed.

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International alliances
Tsukuba
Vocational college of paper
technology and electro mechanics

0%

2.0%

3.0%

• As NaCl
concentration
increases, bacteria
grew less (top), and• fibers swelled less(right).

3.5%
Pure water
NaCl aq. sol.

Vietnam Forestry University

Asian Institute ofTechnology, Thailand

Chulalongkorn
University, Thailand

Zhejiang
University ofScience andTechnology , China

University of Technical
Education Ho Chi Minh City
Royal University ofPhnom Penh, Cambodia
Center for Pulp andPaper, Indonesia

Thank you for attention.

Biomaterial Science by Toshiharu Enomae4