Lumitester

ATP 冷光檢測僅需３步
操作 簡單連接專用軟體
自動完成數據分析

上傳雲端服務實現數據共享龜甲萬百歐凱米發株式會社

ATP+ADP+AMP
冷光素酶
PKATPAMP

ADP

PPDK
ADP
食物殘渣

A3 法 ! 世界首創 !
ATP＋ADP＋AMP!

食物中毒細菌

AMP

ATP組織胺過敏原簡單·快速（１０秒）·數值化 的衛生管理＆現場清洗，您 真 的 做 到 位 了 嗎 ？

使用客群·食品工廠、植物工廠、飲料工廠·中央廚房、餐飲連鎖店、飯店·政府機構、衛生所、學校·醫院、養老院、幼稚園
等

用途·確認現場清洗狀況
·改善清洗方法·監查、巡迴指導
·衛生教育、規範洗手方法等

Because better detection
equals better protection.



Food Service    

Lumitester

TMWithA3 Detection

ATP+ADP+AMP
More Reliable and Sensitive
A3 Finds What Others Miss

Rapid

Rapidly verify sanitation processes on-site
Can be used as on-site improvement tool

Simple & Easy

Easy to operate
Usable for anybody

Numerical

Result is displayed as numeric value
Easy to standardize

Accurate

Detects not only ATP, but also ADP and AMP
Finds what other methods missWhat is A Novel ATP Test？ - ATP+ADP+AMP Hygiene Monitoring System
Kikkoman has developed a novel ATP test, A3 technology by utilizing brewing technique of soy sauce.
Kikkoman's own A3 technology allows you to detect not only ATP but also ADP and AMP have been overlooked.
What is ATP,ADP,AMP?

Kikkoman A3 Technology

ATP (adenosine triphosphate) is a molecule found in all living cells and serves as
an indicator of overall surface hygiene and cleaning Efficacy. ADP (adenosinediphosphate) and AMP (adenosine monophosphate) are derived from ATP
during the processing, such as heat treatment and fermentation.

Kikkoman has forever improved the ATP test using Kikkoman’s A3 Technology that employs advanced chemistry to detect ATP+ADP+AMP to offer
higher sensitivity and better detection.

Object to be measured

PK: Enzyme for the conversion of ADP to ATP.
PPDK: Enzyme for the conversion of AMP to ATP.

Luciferase

ATP recycling enzyme

ATP, ADP, AMP
are present inbacteria, foodresidues

Before CleaningPK

After Cleaning

ATPADP

AMP
ADP

ATP

ADP

ATP

Enzyme for producing lightin the presence of ATP

PPDK

AMP

ADP

Dirt is a mixture of several types of contaminants.

LightAMP

ADP

ATP

Luciferase

AMP

Oxyluciferin

D-Luciferin

PK: pyruvat4e kinase
PPDK: pyruvate orthophoshate dilkinase

When A3-ATP+ADP+AMP level is low, other contaminants such as foodresidues, allergen, microorganisms, virus level are also considered to be low.

A3 Technology - Detects ATP+ADP+AMP / More Reliable and Sensitive*Dilution ratio of test sample

LuciPac A3 detecting
ATP+ADP+AMP, showedsuperior sensitivity andstability for detection offood residues andallergens compared toconventional ATP assays.

400

Milk (Raw)

2,500* X100Whole egg (Raw)* X100

6,000

2,000

300

Wheat flour (Heated)* X100Luminescence Intensity (RLU)

1,000

A3 vs. ATP in Food

04,000Tuna (Raw)* X1,000

0500,000ATP0A3

Shrimp (Raw)

16,000* X1,000

400,000

3,000

1,000
ATPA3Peanuts* X1,000

0

4,000

12,000

3,000

8,000

2,000

4,000

1,000

ATPA3Beer0A30

A3Chocolate* X1,000

3,000
2,000

100,000
ATPATP

4,000

200,000
1,000

0

5,000

* X10

300,000
2,000

Food residues which contain highlevel of ADP, AMP such as meat,
fish, processed food are detectable with high sensitivity.

* X1,000

2,000

500
A3

Chicken (Raw)

3,000

5,000

2,000

ATP

5,000
4,000

4,000200※ATP+ADP+AMP Hygiene Monitoring
System（A3 Assay）is named originallyby Kikkoman.

* X1,000

10,000

1,500

100

15,000Soybeans

0ATPA3ATPA301,000ATPA30ATPA3Reference: “Comparison of Detection Limits for Allergenic Foods between Total Adenylate (ATP+ADP+AMP) Hygiene Monitoring Test and Several Hygiene Monitoring Approaches” M. Bakke et al.., Vol. 83, No. 7

A3 Technology - Indicator of Hygiene Monitoring / Prevents Contamination
Sanitation is critical to food safety. A3 technology prevent ineffective cleaning by monitoring environmental contamination.
Many of the food processors use A3 technology as part of the sanitation program to verify the effectiveness of its processes.
Evaluation of cleaning(stainless surface)

1,000,000

Before cleaning

100,000

Rinse with water

Rinse with hot water

Cleaning with sponge Re-cleaning with sponge
and detergent+rinse and detergent + rinse

100,000

Highly contaminatedby bacteria

10,0001,000
100
10Clean area

High potential forcontamination

Not detectable

1101001,000

10,000100,000

Luminescence intensity by Lumitester(RLU)
Clean

Poor cleaning

A3 Assay
ATP Assay
10,0001,000

Benchmarkvalue

Luminescence intensity (RLU)

Bacterial count by culture procedure（CFU/ml ）Correlation between bacterial counts and ATP+ADP+AMP level

10010Before cleaning No good

Cleanliness level

GoodKikkoman A3 Technology (ATP+ADP+AMP) represents a novel ATP test
that can detect residues left behind by ineffective cleaning at lower levels.

Implementation Guide ̶ Case example of initial stage ̶     
Easy to use and provides immediate feedback and verification of sanitation processes.
Enables monitoring environmental contamination, including food residues andbacteria, and for detection of biofilms that can harbor pathogens and allergens.

Food & Beverage Processors
Evaluate the cleanliness of production line
Determination of test locations
The areas likely remain contaminated such as valves of tankoutlet or pipe-coupling packings
The surface of conveyor belts contact with products or raw
materials directly

Benchmark values and swabbing methods
200 RLU or lower: Smooth / direct contact / easy-to-cleansurfaces (e.g. stainless, glasses)
500 RLU or lower: Unsmooth / indirect contact / hard-to-cleansurfaces (e.g. resin products)
Large sample: Swab 10 cm square area you want to measurevertically and horizontally about 10times for each.
Small sample: Swab the entire area thoroughly

Food Service
Prevent secondary contamination by hygiene monitoring
Determination of test locations
The areas difficult to wash and likely remain contaminated
The areas contact with food that is not sterilized (raw food)Benchmark values and swabbing methods
200 RLU or lower: Smooth / direct contact / easy-to-cleansurfaces (e.g. stainless, glasses)
500 RLU or lower: Unsmooth / indirect contact / hard-to-cleansurfaces (e.g. resin products)
Large sample: Swab 10 cm square area you want to measurevertically and horizontally about 10times for each.
Small sample: Swab the entire area thoroughly

Hygiene Education and Feedback
Conduct hygiene education for employees
Encourage hand washing by training and feedback

Kikkoman Case Studies

LuciPac A3 can be used for daily evaluation ofcleaning, also allows for users can detect
especially contaminated locations.
The risk of presence of residual allergens can bedecreased by thorough washing.
Benchmark values

Swabbing method

Test points

lower/pass | upper/failFiller / Nozzle

200 | 400Tank

200 | 400

Pipe

200 | 400

ValveProcessing equipment

200 | 400

Swab the inner surface and make circles 2 to 3 times, while turning the cottonswab around
Inner side such as disk, seat(e.g. meat processing equipment)

500 | 1000

10 cm square around the center

Production line

500 | 1000

10 cm square around the center(e.g. conveyer)

Swab the inner surface and make circles 2 to 3 times, while turning the cottonswab around
10 cm square of the center bottom portion and top end portion of the inside※When you make a measurement, it needs to be performed after cleaning.

You can check if cleaning is properly done withon site measurement, then prevent the accident
with re-cleaning.
A numerical display of test results allows
effective cleanliness management, comparing
readings from different shops, sites, etc
Benchmark values Swabbing method

Test points

lower/pass | upper/failKnife
Cutting board
Counter / Table200 | 400
500 | 1000
200 | 400

Both sides of the entire blade, a handle, joint between the blade and the handle
Swab a 10 cm by 10 cm area at the center in the left-to right and up-to-down directions
Swab a 10 cm by 10 cm area at the center in both the left-to-right and up-and-down
directions

Refrigerator handle

500 | 1000

Swab the entire surface of the handle※When you make a measurement, it needs to be performed after cleaning.

Hand washing is primarymeasure of infection
prevention and control!

When do you measure?
Please use this kit after hand washing and before using
disinfectants.

Benchmark values and swabbing methods
2000 RLU or lower※: After hand washing.
Swab entire palm including fingertips and between fingers,etc.

Note Please note that benchmark RLU value depends on thecondition of the surface, and type of ingredients/materials
contacted on the surface, also risk management level of the area.
We recommend our customer to conduct self-validation andestablish own benchmark value.

Test points
Hand washing / Hand and Fingers

Benchmark values

lower/pass | upper/fail2000 | 4000

Swabbing method
Swab the entire palm of the hand over 5-10passes in the left-to-right andup-to-down directions as well as between fingers and the tips of fingers※90% of people can achieve this level when they conduct A3 test after hand washing.
Since the previous model, LuciPac Pen, detects ATP+AMP, the benchmark value is 1500 RLU.



AppLumitester

With the App, anyone can check cleanliness easily
Measure    Swab the sample and insert
the swab into main body

Shake well and insert into theLumitester

Measurements (RLU)
displayed within 10 seconds

RESULT

Knife 2

Understand     
Recorded data can be displayed in time-series byregistering with our specialized App. Inspection passrates are automatically graphed and improvements
can be visualized. Employees will be more consciousof hygiene, and will maintain a high standard ofenvironmental sanitation, which will help build
greater trust in your businessConnect 

   

Display time-series data for each inspection      

Visualize overall inspection scores through graphs  

Data will be stored in the cloud and can be accessed fromanywhere in the world. This will allow fast detection andresponse to any problems that may arise.

Rapid Hygiene Monitioring System

ATP+ADP+AMP Test

Lumitester Smart Product code: 61234

TMMeasurement time: 10 seconds
Data output: RLU (Relative Light Unit)
Power: Two AA alkaline or nickel hydride rechargeable batteries
Accessories: Two AA alkaline batteries, cleaning brush, USB cable,strap, Quick Manual※ Do not use this product for purposes other than hygiene monitoring.
※ It is not to be used for counting general living bacteria or detecting specific pathogens.

2-1-1 Nishi-Shinbashi, Minato-ku, Tokyo 105-0003 JAPANTEL : +81-3-5521-5481 FAX : +81-3-5521-5498
biochemifa@mail.kikkoman.co.jp
https://biochemifa.kikkoman.co.jp/e/https://biochemifa.kikkoman.co.jp/e/kit/atp/※ The information contained in this leaflet is subject to change without further notice.

© 2020 Kikkoman Corp. (1512C200801)

LuciPac A3 Surface
LuciPac A3 Water
TMTM

Learn about A3 Technology

LuciPac

TM

Product code: 60361 100 swabs/kit
Product code: 60365 100 swabs/kit

Storage Condition: 2-8°C (Do not freeze)
25°C: 14 days (Unopened)
30°C: 5 days (Unopened)
Expiry: 15 months after manufacturing date※ Use LuciPac A3 for Lumitester Smart , PD-30 or PD-20. Do not use it for other models.