Niigata Dent. J. 45（2）：13 － 19,－原著－

2015

51

トレハロースタブレットの口腔保湿に対する効果の分析

川﨑真依子 1），鈴木亜夕帆 2），坂井裕次 3），相田美和 4），五十嵐敦子 5），渡邊智子 2）1）

新潟大学大学院医歯学総合研究科 生体歯科補綴学分野
2）

千葉県立保健医療大学健康科学部栄養学科

3）

株式会社ブルボン製品開発部 海外製品開発課

4）

新潟大学大学院医歯学総合研究科 口腔生化学分野
5）

新潟大学歯学部 口腔生命福祉学科

Analysis of Oral Moisturizing Effects of Trehalose Tablets
Maiko Kawasaki 1）, Ayuho Suzuki 2）, Yuji Sakai 3）, Miwa Sohda 4）, Atsuko Igarashi 5）,
Tomoko Watanabe 2）
1)

Divisions of Bio-Prosthodontics, Niigata University Graduate School of Medical and Dental Sciences.

2)

Department of Nutrition Faculty of Health Science, Chiba Prefectural University of Health Sciences.
3)
4)

Bourbon Corporation Product Development Center.

Divisions of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences.
5)

Department of Oral Health and Welfare, Faculty of Dentistry, Niigata University.
平成 27 年７月９日受付

平成 27 年８月６日受理

キーワード：トレハロース，保湿，加齢Key words: trehalose, moisturization, aging
Abstract
In recent years, the elderly population has been growing, and subsequently, the number of patients complainingof xerostomia symptoms has been increasing. In this study, we focused on the moisturizing effects of trehalose.
We developed a trehalose tablet as a novel supplement for xerostomia and compared it to a sucrose tablet forverification of its moisturizing effects. A total of 72 healthy female subjects were randomly assigned to receive
either the sucrose or trehalose tablet, followed by the other. Subjects received a 14-question survey soon after
each tablet had dissolved completely in the mouth. For objective evaluation of the tablets, compression andsolubility tests were performed. Based on sensory (subjective) evaluation, the trehalose tablets provided
significantly more moisturization in the mouth and a sense of salivation than the sucrose tablets. Therefore,
trehalose may confer a greater effect on moisturizing the mouth than sucrose. In addition, the trehalose tablets
were about 2/3 as soft and dissolved 2 to 3 times more quickly than the sucrose tablets. These subjective andobjective evaluations suggest that trehalose is a better supplement for maintaining oral moisture than sucrose,and may therefore be useful as a therapeutic supplement. The use of this convenient and safe therapeutic
supplement could help improve the quality of life of elderly individuals who are suffering from xerostomia byallowing them to better enjoy meals and conversation.
和文抄録
近年，高齢者の急激な増加に伴い，口腔乾燥症の症状を訴える患者も増加の一途を辿っている。本研究では，トレ
ハロースの保湿効果に着目し，口腔乾燥のための新しい治療補助食品として，トレハロースタブレットを開発した。
保湿効果の比較の為に蔗糖のタブレットも同時に開発し，効果について主観的，客観的に検証を行った。実験には72 名の健常な女性に参加して頂き，タブレットを試食して口腔内で完全に溶解して消失してから，その後 14 項目の－ 13 －

新潟歯学会誌

5245（2）：2015

アンケート調査を実施した。また，タブレットの物性について客観的評価として，圧縮試験及び溶解度試験も合わせて実施した。主観的評価結果から，トレハロースが蔗糖と比較して，有意に口腔内の保湿に影響を与えている可能性が示された。また，客観的評価より，トレハロースタブレットは，蔗糖タブレットと比較して 2 ／ 3 程度軟らかく，口腔内で 2 ～ 3 倍早く溶解することが示された。これらの結果より，トレハロースタブレットは，口腔内の保湿に寄与することが示唆された。このことから，口腔内の乾燥で悩む高齢者に対しても効果が期待でき，食事や会話への楽しみ，QOL の向上へと繋げていく可能性が示唆された。

we enrolled 72 healthy female subjects with a mean

Introduction

age of 30.28±7.87 years. Informed consent wasobtained from all subjects. Because this was a primary

In recent years, the elderly population has been

experiment, individuals who were currently suffering

growing rapidly, and accordingly, the number offrom dry mouth were excluded.

patients complaining of xerostomia symptoms has been

2. Methods

increasing. Xerostomia is an oral disease in which

Subjects were randomly assigned with a number todecreased saliva production results in oral dryness,determine the order of tablet sampling. The even-possibly as a result of adverse drug reactions, aging,numbered subjects were first directed to take adecreased muscular strength, the effects of diseases

sucrose tablet, followed by a trehalose tablet, while theodd-numbered subjects were first directed to have a1)

such as Sjögren’s syndrome, diabetes and stress.

Treatments for xerostomia have generally included

trehalose tablet followed by a sucrose tablet. Subjects

mouthwashes, lozenges, oral ointments, artificial saliva

were blinded to the ingredients of the tablets and theand oral drugs. However, these treatments fail toorder in which they were taken. The two samples

alleviate xerostomia symptoms resulting from multiple

were labeled “A” for sucrose and “B” for trehalosefactors in some elderly patients, causing much concern(Figure 1). Between sampling, subjects rested for 5 minover the appropriate response.

and rinsed to exclude bias based on the order ofTrehalose is a nonreducing sugar with two glucoses

sampling. To exclude the effects of differences in tablet

joined by an α,α-1,1-glucosidic bond that is not founddissolution time, subjects were asked to spit out thein starch. Furthermore, trehalose has anti-cariogenic

remaining portion of the tablets that had not dissolved

characteristics. With a sweetness 45% that of sugar,

in the mouth after 1 min. Subjects received atrehalose is more frequently being used in Japanese

14-question survey soon after the second tablet hadand Western confectionery and other types ofdissolved completely in the mouth (Table 1). Questions

2)

cooking.

3), 4)

It can also be used to protect proteins and1-4 evaluated both tablets (sucrose or trehalose) withcells against drying and freezing by replacing water.5), 6)
There has been a report that adding trehalose tobacteria 7) or transferring trehalose synthase into
human fibroblasts confers a tolerance to desiccation.8)Trehalose is therefore often used as a moisturizer incosmetics and other products.9), 10)
In this study, we focused on the moisturizing effectsof trehalose and developed a trehalose tablet as a new
supplement for treatment of xerostomia. We
concurrently developed a sucrose tablet for comparing
and verifying various aspects of these moisturizingeffects.

Materials and Methods
1. Subjects
As xerostomia is a common ailment among women,－ 14 －

A: Sucrose, B: Trehalose

Figure 1: Trehalose & Sucrose samples