June 24, 2014

Biomaterial Science（Schedule）
#Date

14/15

History of papermaking
Pulps – Beating and fiber properties

ContentBio-Material Science

24/22

35/9, Fri Pulps – Additives and functions

Toshiharu Enomae

45/13

Papermaking processes & interfiber bonding

Professor, PhD, Paper Device and Eco-friendly materials

55/20

Paper– Structural properties

65/27

Paper– Surface properties

76/3

Polysaccharide chemistry by Assoc Prof Akiko Nakagawa

86/10

Paper–Wetting and absorption properties

96/17

Paper– Mechanical and optical properties

106/24

Recent trend of paper science and technology

EG60411

2G103, 10:10-11:25, Tuesday

Prospective future with
Paper devices andEco-friendly materials

 Paper device（Paper in future）• Paper electronics• Paper–based medical check-up sensor• Energy supply device• Paper-based bioassay system

Toshiharu EnomaeProfessor
Faculty of Life and Environmental Sciences
University of Tsukuba , JAPAN Paper cultural heritage（Paper in past）• Conservation Science Fundamental papermaking technology（Paper at present）• Paper coating, paper physics and chemistry, etc.

Development of Paper-based medical
check-up sensor and technology ofliquid transport in a micro-channel

Doctor in hospital
DataKento Maejima
Yinchao Xu
Toshiharu Enomae

Yinchao Xu

Biomaterial Science by Toshiharu EnomaeDiagnosis
Patient at home・Fewer patients in hospital for fewer days
→ Appropriate medical service by hospital

Affordable and simple device is required1June 24, 2014

Advantage of paper and improvement

Paper substrate• Cheap, disposable, portable, and flexible• Cotton linter pulp→ almost no impurities• Porous → micro-channel to transport liquid• Smaller pore size → Less ink bleeding• Capillary force due to porous structure• Liquid transport
designed channel
Lab-on-paper as a medical check-up sensor#Beating,
revolutions-PFI millDensity,
g/cm3

1100000.568

2200000.622

3300000.641
193

Fabrication Processes of electrodes

Microchannel Fabrication
IJ printing

Electrodes printed with PEDOT:PSS

UV irradiation

Paper

Prepared paperTopTop

Micro-channel printedwith UV-curable ink
BMP printed
Enzyme (Glucose oxidase) printed

Perspective
BackBackTop

EnzymeBack

Microchannel was successfully fabricatedCurrent (t=10 s) [mA]

Dispense195Potentiostat

Micro-channel
printed30 mm(4 μL)

Ink jet printer

1.2
y = 0.160 x + 0.203
R² = 0.979

0.60.3Lab-on-Paper was successfully fabricated

4 mm

Sample solution• 0.5 M KCl• 2 mM K3[Fe(CN)6]• 15 U/mL HRP• Glucose Conc.
(0-4.16 mM)
0.9

PEDOT:PSS (electrode)

3 mm

Citterio, D. et al., Proc. MicroTAS 2011, 2099-2101

BMP (binder&mediator)

n=30012
3
45
Glucose Concentration [mmol]

Glucose + O2 
Gluconic acid + H+ + eGlucose concentration
quantified successfullyBiomaterial Science by Toshiharu Enomae

Dimatix DMP-2831
Fuji Film, JAPANInk• Styrene-acrylate copolymer, CP-900, Harima Chemical, JAPAN• Neutral red (dye staff)

2June 24, 2014

Laboratory of Paper Devices and Eco-friendly Materials
Prof. Toshiharu Enomae, University of Tsukuba

Effect of beating

910000 Rev

0.1
0.08 2000 Rev

Effect of calendering
Conversion of vibrationenergy of paper toelectric energy

875000 Rev

6Volume (μL)

Volume/ Grammage (μL/g/m2)

0.12

0.06

5430.04

Uncalendered
0.5 MPa
1 MPa
3 MPa

20.02
1000200

4006008001000050Time (s)Lucas-Washburn’sequation: V ∝ rp

Beatingrp

Calendering rp

Moving-electrodeMoving-electrode

Electrode (Copper)
100Time (s)rpWatertransport rp

150200Paper

Irreversible
Reversible

Electret (Teflon)
Electrode (Copper)～

Electretii
RR

Static-electrodeVibration

Paper-made Electric Power Generator

Bacterial culture system using paper
substrate and ink jet printing
Tithimanan Srimongkon
Toshiharu Enomae

Application to:
Voice-activated posters

Ink jet printer dispenses

Stack of Petri dishes fordifferent conditions!!

• constant number ofcells,• same patterns, and• a few environments in
one medium.

Biomaterial Science by Toshiharu Enomae

Agar sheet medium

3June 24, 2014

Agar medium

48 hours after printing
Printing
Liquid agar medium

Dot of E. coli colony

Agar sheet medium

Hydrophilic
Hydrophobic

Bacteria

Observation

Bacteria printing

Influence of saltwater immersion onproperties of flood-damaged paper
Tunchira Bunyaphiphat

0 hr

12 hrs

24 hrsAkiko Nakagawa-Izumi
Toshiharu Enomae• Bacteria grew
successfully.
• Agar maintained
moisture for 24 h
48 hrs36 hrsMold grows and• Characters cannot beread due to color• Bad smell• Health hazard
Flood-damaged paper and books • Artistic value lost
To inhibit mold growth
WashingDrying

Restoration… Speedy action is difficult!

Immersion in saltwater

0%

2.0%

• As NaCl
concentration
increases, bacteria
grew less (top), and• fibers swelled less(right).

3.0%

3.5%
Pure water
NaCl aq. sol.

was proposed.

Biomaterial Science by Toshiharu Enomae

4June 24, 2014

International alliances
Tsukuba
Vocational college of paper
technology and electro mechanics
Vietnam Forestry University

Asian Institute ofTechnology, Thailand

Chulalongkorn
University, Thailand

Zhejiang
University ofScience andTechnology , China

Thank you for attention.

University of Technical
Education Ho Chi Minh City
Royal University ofPhnom Penh, Cambodia
Center for Pulp andPaper, Indonesia

Biomaterial Science by Toshiharu Enomae

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