Daisuke Fujinami
Department of Food and Nutritional
Sciences, University of Shizuoka, Shizuoka, Yada 52-1, Sugura-ku,Shizuoka 422-8526, Japan
E-mail:dfujinami@u-shizuoka-ken.ac.jp2023/02/16

Education
2011

Bachelor of Science, Kyushu University, Japan
Advisor: Prof. Daisuke Kohda

2017

Ph.D., Kyushu University, Japan
Advisor: Prof. Daisuke Kohda

Positions since final degree
2017

Postdoctoral Fellow, Medical Institute of Bioregulation, Kyushu University,
Japan
Advisor: Prof. Daisuke Kohda

2019

Postdoctoral Fellow, Department of Chemistry, University of Illinois at UrbanaChampaign, Illinois
Advisor: Prof. Wilfred A. van der Donk

2021

Postdoctoral Fellow, Graduate school of Pharmaceutical Sciences, The Universityof Tokyo, Japan
Advisor: Prof. Ikuro Abe

2022

Assistant professor, Department of Food Science and Biotechnology, University ofShizuoka, Japan

Affiliations
The Nuclear Magnetic Resonance Society of Japan

Fellowships and Awards
Uehara Memorial Foundation postdoctoral fellowship (2020)
Grant for Studying Overseas from The Naito Foundation (2019)

Publications
Kohda D, Hayashi S, Fujinami D. Residue-based quadratic free energy relationship is amathematical formulation of the consistency principle of protein folding. bioRxiv
doi:10.1101/2022.10.24.513469

Fujinami D, Hayashi S, Kohda D. Retrospective study for the universal applicability of
the residue-based linear free energy relationship in the two-state exchange of protein
molecules. Sci Rep. 2022 Oct 7;12(1):16843.

Fujinami D, Hayashi S, Kohda D. Residue-Specific Kinetic Insights into the Transition
State in Slow Polypeptide Topological Isomerization by NMR Exchange Spectroscopy. JPhys Chem Lett. 2021 Nov 4;12(43):10551-10557.

Fujinami D, Garcia de Gonzalo CV, Biswas S, Hao Y, Wang H, Garg N, Lukk T, NairSK, van der Donk WA. Structural and mechanistic investigations of protein Sglycosyltransferases. Cell Chem Biol. 2021 Dec 16;28(12):1740-1749.e6.

Inoue N, Terabayashi T, Takiguchi-Kawashima Y, Fujinami D, Matsuoka S, Kawano M,Tanaka K, Tsumura H, Ishizaki T, Narahara H, Kohda D, Nishida Y, Hanada K. The
benzylisoquinoline alkaloids, berberine and coptisine, act against camptothecinresistant topoisomerase I mutants. Sci Rep. 2021 Apr 8;11(1):7718.

Kawasaki Y, Ariyama H, Motomura H, Fujinami D, Noshiro D, Ando T, Kohda D. TwoState Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase
Observed in Real-Time by High-Speed AFM. J Mol Biol. 2020 Nov6;432(22):5951-5965.

Fujinami D, Motomura H, Oshima H, Mahin AA, Elsayed KM, Zendo T, Sugita Y,
Sonomoto K, Kohda D. Mosaic Cooperativity in Slow Polypeptide Topological
Isomerization Revealed by Residue-Specific NMR Thermodynamic Analysis. J PhysChem Lett. 2020 Mar 5;11(5):1934-1939.

Fujinami D, -Mahin AA, Elsayed KM, Islam MR, Nagao JI, Roy U, Momin S, Zendo T,Kohda D, Sonomoto K. The lantibiotic nukacin ISK-1 exists in an equilibrium between
active and inactive lipid-II binding states. Commun Biol. 2018 Sep 25;1:150.

Fujinami D, Taguchi Y, Kohda D. Asn-linked oligosaccharide chain of a crenarchaeon,Pyrobaculum calidifontis, is reminiscent of the eukaryotic high-mannose-type glycan.
Glycobiology. 2017 Aug 1;27(8):701-712.

Taguchi Y, Fujinami D, Kohda D. Comparative Analysis of Archaeal Lipid-linked
Oligosaccharides That Serve as Oligosaccharide Donors for Asn Glycosylation. J BiolChem. 2016 May 20;291(21):11042-54.

Fujinami D, Nyirenda J, Matsumoto S, Kohda D. Structural elucidation of an
asparagine-linked oligosaccharide from the hyperthermophilic archaeon,Archaeoglobus fulgidus. Carbohydr Res. 2015 Sep 2;413:55-62.

Fujinami D, Matsumoto M, Noguchi T, Sonomoto K, Kohda D. Structural elucidation ofan asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus
furiosus. Carbohydr Res. 2014 Mar 31;387:30-6.