研究業績
原著論文
1. Takagi M, Kuzuyama T, Takahashi S and Seto H. A gene cluster for the mevalonate pathway from
Streptomyces sp. strain CL190. J Bacteriol. 182, 4153-4157 (2000)
2. Kuzuyama T, Takahashi S, Takagi M and Seto H. Characterization of 1-deoxy-D-xylulose 5-phosphate
reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalyticamino acid residues. J Biol Chem. 275, 19928-19932 (2000)
3. Takagi M, Kuzuyama T, Kaneda K, Watanabe H, Dairi T and Seto H. Studies on the nonmevalonate pathway:
Formationof2-C-methyl-D-erythritol

2,4-cyclodiphosphate

from2-phospho-4-(cytidine

5'-diphospho)-2-C-methyl-D-erythritol. Tetrahedron Lett. 41, 3395-3398 (2000)
4. Kuzuyama T, Takagi M, Kaneda K, Watanabe H, Dairi T and Seto H. Studies on the nonmevalonate pathway:
Conversion of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol to its 2-phospho derivative by 4-(cytidine
5'-diphospho)-2-C-methyl-D-erythritol kinase. Tetrahedron Lett. 41, 2925-2928 (2000)
5.

Kuzuyama

T,

TakagiM,

Kaneda

K,

Dairi

T

and

Seto

H.

Formationof

4-(cytidine

5'-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol
4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway. Tetrahedron Lett. 41, 703-706(2000)
6. Kuzuyama T, Takagi M, Takahashi S and Seto H. Cloning and characterization of 1-deoxy-D-xylulose
5-phosphate synthase from Streptomyces sp. strain CL190, which uses both the mevalonate and nonmevalonate
pathways for isopentenyl diphosphate biosynthesis. J Bacteritol. 182, 891-897 (2000)
7. Kakinuma K, Dekishima Y, Matsushima Y, Eguchi T, Misawa N, Takagi M, Kuzuyama T and Seto H. New
approach to multiply deuterated isoprenoids using triply engineered Escherichia coli and its potential as a tool
for mechanistic enzymology. J Am Chem Soc. 123, 1238-1239 (2001)
8. Kaneda K, Kuzuyama T, Takagi M, Hayakawa Y and Seto H. A new type of isopentenyl diphosphate
isomerase. Proc Natl Acad Sci USA. 98, 932-937 (2001)
9. Kasahara H, Hanada A, Kuzuyama T, Takagi M, Kamiya Y and Yamaguchi S. Contribution of the mevalonate
and methylerythritol phosphate pathways to the biosynthesis of gibberellins in Arabidopsis. J Biol Chem. 277,1

45188-45194 (2002)
10. Kishida H, Wada T, Unzai S, Kuzuyama T, Takagi M, Terada T, Shirouzu M, Yokoyama S, Tame JR and ParkSY. Structure and catalytic mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) synthase, an
enzyme in the non-mevalonate pathway of isoprenoid synthesis. Acta Crystallogr D Biol Crystallogr. 59, 23-31(2003)
11. Takagi M, Kaneda K, Shimizu T, Hayakawa Y, Seto H and Kuzuyama T. Bacillus subtilis ypgA gene is fni, anonessential gene encoding Type 2 isopentenyl diphosphate isomerase. Biosci Biotechnol Biochem. 68, 132-137(2004)
12. Tera M, Sohtome Y, Ishizuka H, Doi T, Takagi M, Shin-ya K and Nagasawa K. Design and synthesis oftelomestatin derivatives and their inhibitory activity of telomerase. Heterocycles. 69, 505-514 (2006)
13. Sato A, Takagi M, Shimamoto A, Kawakami S and Hashida M. Small interfering RNA delivery to the liverby intravenous administration of galactosylated cationic liposomes in mice. Biomaterials. 28, 1434-1442 (2007)
14. Ueda JY, Hashimoto J, Nagai A, Nakashima T, Komaki H, Anzai K, Harayama S, Doi T, Takahashi T,
Nagasawa K, Natsume T, Takagi M* and Shin-ya K New aureothin derivative, alloaureothin, from Streptomyces
sp. MM23. J Antibiot. 60, 321-324 (2007)
15. Sato A, Choi SW, Hirai M, Yamayoshi A, Moriyama R, Yamano T, Takagi M, Kano A, Shimamoto A andMaruyama A. Polymer brush-stabilized polyplex for a siRNA carrier with long circulatory half-life. J Control
Release. 122, 209-216 (2007)
16. Oishi M, Nagasaki Y, Nishiyama N, Itaka K, Takagi M, Shimamoto A, Furuichi Y and Kataoka K. Enhanced
growth inhibition of hepatic multicellular tumor spheroids by lactosylated poly(ethylene glycol)-siRNA
conjugate formulated in PEGylated polyplexes. ChemMedChem. 2, 1290-1297 (2007)
17. Ueda JY, Togashi T, Matukura S, Nagai A, Nakashima T, Komaki H, Anzai K, Harayama S, Doi T, Takahashi
T, Natsume T, Kisu Y, Goshima N, Nomura N, Takagi M* and Shin-ya K. A novel nuclear export inhibitor
JBIR-02, a new piericidin discovered from Streptomyces sp. ML55. J Antibiot. 60, 459-462 (2007)
18. Izumikawa M, Ueda JY, Chijiwa S, Takagi M* and Shin-ya K. Novel GRP78 molecular chaperone
expression down-regulators JBIR-04 and -05 isolated from Streptomyces violaceoniger. J Antibiot. 60, 640-6442(2007)
19. Ogata M, Ueda JY, Hoshi M, Hashimoto J, Nakashima T, Anzai K, Takagi M* and Shin-ya K. A novel
indole-diterpenoid, JBIR-03 with anti-MRSA activity from Dichotomomyces cejpii var. cejpii NBRC 103559.
J Antibiot. 60, 645-648 (2007)
20. Umeda Y, Furihata K, Sakuda S, Nagasawa H, Ishigami K, Watanabe H, Izumikawa M, Takagi M, Doi T,
Nakao Y and Shin-ya K. Absolute structure of prunustatin A, a novel GRP78 molecular chaperone
down-regulator. Org Lett. 9, 4239-4242 (2007)
21. Futami K, Takagi M, Shimamoto A, Sugimoto M and Furuichi Y. Increased chemotherapeutic activity ofcamptothecin in cancer cells by siRNA-induced silencing of WRN helicase. Biol Pharm Bull. 30, 1958-1961(2007)
22. Futami K, Kumagai E, Makino H, Goto H, Takagi M, Shimamoto A and Furuichi Y. Induction of mitotic cell
death in cancer cells by small interference RNA suppressing the expression of RecQL1 helicase. Cancer Sci. 99,
71-80 (2008)
23. Futami K, Kumagai E, Makino H, Sato A, Takagi M, Shimamoto A and Furuichi Y. Anticancer activity of
RecQL1 helicase siRNA suppression in mouse xenograft models. Cancer Sci. 99, 241-244 (2008)
24. Tera M, Ishizuka H, Takagi M, Suganuma M, Shin-ya K and Nagasawa K. Macrocyclic hexaoxazoles as
sequence- and mode-selective G-quadruplex binders. Angew Chem Int Ed Engl. 47, 5557-5560 (2008)
25. Ueda JY, Nagai A, Izumikawa M, Chijiwa S, Takagi M* and Shin-ya K. A novel antimycin-like compound,
JBIR-06, from Streptomyces sp. ML55. J Antibiot. 61, 241-244 (2008)
26. Izumikawa M, Komaki H, Hashimoto J, Takagi M* and Shin-ya K. Stawamycin analog, JBIR-11 from
Streptomyces viridochromogenes subsp. sulfomycini NBRC 13830. J.Antibiot. 61, 326-329 (2008)
27. Numajiri N, Takahashi T, Takagi M, Shin-ya K and Takayuki D. Total Synthesis of largazole and its
biological evaluation. Synlett. 16, 2483-2486 (2008)
28. Ueda JY, Chijiwa S, Takagi M* and Shin-ya K. A novel versipelostatin analogue, versipelostatin F isolated
from Streptomyces versipellis 4083-SVS6. J Antibiot. 61, 752-755 (2008)3

29. Izumikawa M, Ukai H, Takagi M,* Ueda HR and Shin-ya K. JBIR-26, a novel natural compound from
Streptomyces sp. AK-AH76, regulates mammalian circadian clock. J Antibiot. 61, 756-758 (2008)
30. Motohashi K, Hwang JH, Sekido Y, Takagi M* and Shin-ya K. JBIR-23 and -24, novel anticancer agentsfrom Streptomyces sp. AK-AB27. Org Lett. 11, 285-288 (2009)
31. Tera M, Iida K, Ishizuka H, Takagi M, Suganuma M, Doi T, Shin-ya K and Nagasawa K. Synthesis of apotent G-quadruplex-binding macrocyclic heptaoxazole. Chembiochem. 10, 431-435 (2009)
32. Komaki H, Izumikawa M, Ueda JY, Nakashima T, Khan ST, Takagi M and Shin-ya K. Discovery of apimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type I
polyketide synthase gene. Appl Microbiol Biotechnol. 83, 127-133 (2009)
33. Murakami R, Shinozaki J, Kajiura T, Kozone I, Takagi M, Shin-ya K, Seto H and Hayakawa Y. Ammocidins
B, C and D, new cytotoxic 20-membered macrolides from Saccharothrix sp. AJ9571. J Antibiot. 62, 123-127(2009)
34. Kozone I, Ueda JY, Watanabe M, Nogami S, Nagai A, Inaba S, Ohya Y, Takagi M* and Shin-ya K. Novel
24-membered macrolides, JBIR-19 and -20 isolated from Metarhizium sp. fE61. J Antibiot. 62, 159-162 (2009)
35. Ueoka R, Ito A, Izumikawa M, Maeda S, Takagi M, Shin-ya K, Yoshida M, van Soest RW and Matsunaga S.
Isolation of azaspiracid-2 from a marine sponge Echinoclathria sp. as a potent cytotoxin. Toxicon. 53, 680-684(2009)
36. Izumikawa M, Nagai A, Doi T, Takagi M* and Shin-ya K. JBIR-12, a novel antioxidative agent from
Penicillium sp. NBRC 103941. J Antibiot. 62, 177-180 (2009)
37. Motohashi K, Hashimoto J, Inaba S, Khan ST, Komaki H, Nagai A, Takagi M* and Shin-ya K. New
sesquiterpenes, JBIR-27 and -28, isolated from a tunicate-derived fungus, Penicillium sp. SS080624SCf1. J
Antibiot. 62, 247-250 (2009)
38. Zhao P, Ueda JY, Kozone I, Chijiwa S, Takagi M, Kudo F, Nishiyama M, Shin-ya K and Kuzuyama T. New
glycosylated derivatives of versipelostatin, the GRP78/Bip molecular chaperone down-regulator, from
Streptomyces versipellis 4083-SVS6. Org Biomol Chem. 7, 1454-1460 (2009)4

39. Ueda JY, Hwang JH, Maeda S, Kato T, Ochiai A, Isshiki K, Yoshida M, Takagi M* and Shin-ya K. JBIR-17,
a novel trichostatin analog from Streptomyces sp. 26634. J Antibiot. 62, 283-285 (2009)
40. Tanaka H, Yoshizawa A, Chijiwa S, Ueda JY, Takagi M, Shin-ya K and Takahashi T. Efficient synthesis of
the deoxysugar Part of versipelostatin by direct and stereoselective glycosylation and revision of the structure of
the trisaccharide Unit. Chem. Asian J. 6, 1114-1125 (2009)
41. Khan ST, Harayama S, Tamura T, Ando K, Takagi M and Shin-ya K. Paraoerskovia marina gen. nov., sp.
nov., a novel actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol. 59, 2094-2098 (2009)
42. Fujiwara T, Hwang JH, Kanamoto A, Nagai H, Takagi M* and Shin-ya K. JBIR-44, a new bromotyrosine
compound from a marine sponge Psammaplysilla purpurea. J. Antibiot. 62, 393-395 (2009)
43. Motohashi K, Inaba S, Takagi M* and Shin-ya K. JBIR-15, a new aspochracin derivative, isolated from asponge-derived fungus, Aspergillus sclerotiorum Huber Sp080903f04. Biosci Biotechnol Biochem. 73,
1898-1900 (2009)44. Hashimoto J, Watanabe T, Seki T, Karasawa S, Izumikawa M, Seki T, Iemura SI, Natsume T, Nomura N,
Goshima N, Miyawaki A, Takagi M* and Shin-ya K. Novel in vitro protein fragment complementation assay
applicable to high-throughput screening in a 1536-well format. J Biomol Screen. 14, 970-979 (2009)
45. Izumikawa M, Khan ST, Komaki H, Nagai A, Inaba S, Takagi M* and Shin-ya K. JBIR-37 and -38, novel
glycosyl benzenediols, isolated from the sponge-derived fungus, Acremonium sp. SpF080624G1f01. Biosci
Biotechnol Biochem. 73, 1898-1900 (2009)
46. Kozone I, Ueda JY, Takagi M* and Shin-ya K. JBIR-52, a new antimycin-like compound, from Streptomyces
sp. ML55. J Antibiot. 62, 593-595 (2009)
47. Mukai A, Komaki H, Takagi M* and Shin-ya K. Novel siderophore, JBIR-16, isolated from Nocardia
tenerifensis NBRC 101015. J Antibiot. 62, 601-603 (2009)
48. Ueda JY, Takagi M* and Shin-ya. Two novel decanedienamide derivatives, JBIR-07 and -08, from
Streptomyces versipellis 4083-SVS6. Nat Prod Res. 23, 1337-1341 (2009)5

49. Hashimoto J, Motohashi K, Sakamoto K, Hashimoto S, Yamanouchi M, Tanaka H, Takahashi T, Takagi M*
and Shin-ya K. Screening and evaluation of new inhibitors of hepatic glucose production. J Antibiot. 62, 625-629(2009)
50. Motohashi K, Gyobu Y, Takagi M* and Shin-ya K. JBIR-25, a novel antioxidative agent from Hyphomycetes
sp. CR28109. J Antibiot. 62, 703-704 (2009)
51. Mukai A, Nagai A, Inaba S, Takagi M* and Shin-ya K. JBIR-54, a new 4-pyridinone derivative isolated from
Penicillium daleae Zaleski fE50. J Antibiot. 62, 705-706 (2009)
52. Ueda JY, Takagi M* and Shin-ya K. Aminocaprophenone- and pyrrolidine-type alkaloids from the leaves ofFicus septica. J Nat Prod. 72, 2181-2183 (2009)
53. Izumikawa M, Khan ST, Komaki H, Takagi M* and Shin-ya K. JBIR-31, a new teleocidin analog, producedby salt-requiring Streptomyces sp. NBRC 105896 isolated from a marine sponge. J Antibiot. 63, 33-36 (2010)
54. Khan ST, Izumikawa M, Motohashi K, Mukai A, Takagi M* and Shin-ya K. Distribution of the
3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived
Actinobacteria. FEMS Microbiol Lett. 304, 89-96 (2010)
55. Fujiwara T, Nagai A, Takagi M* and Shin-ya K. JBIR-69, a new metabolite from Streptomyces sp. OG05. J
Antibiot. 63, 95-96 (2010)
56. Ueda JY, Kunoh T, Sekigawa M, Wada SI, Mukai Y, Ohta S, Sasaki R, Mizukami T, Takagi M* and Shin-ya
K. JBIR-14, a highly oxygenated ergostane, from Isaria sp. NBRC 104353. J Antibiot. 63, 139-141 (2010)
57. Izumikawa M, Khan ST, Takagi M* and Shin-ya K. Sponge-derived Streptomyces producing isoprenoids via
the mevalonate pathway. J Nat Prod. 73, 208-212 (2010)
58. Motohashi K, Takagi M* and Shin-ya K. Tetrapeptides possessing a unique skeleton, JBIR-34 and JBIR-35,
isolated from a sponge-derived actinomycete, Streptomyces sp. Sp080513GE-23. J Nat Prod. 73, 226-228 (2010)
59. Izumikawa M, Hashimoto J, Hirokawa T, Sugimoto S, Kato T, Takagi M* and Shin-ya K. JBIR-22, an
inhibitor for protein-protein interaction of the homodimer of proteasome assembly factor 3. J Nat Prod. 73,
628-631 (2010)6

60. Motohashi K, Takagi M* and Shin-ya K. Tetracenoquinocin and 5-iminoaranciamycin from a sponge-derived
Streptomyces sp. Sp080513GE-26. J Nat Prod. 73, 755-758 (2010)
61. Ueda JY, Khan ST, Takagi M* and Shin-ya K. JBIR-58, a new salicylamide derivative, isolated from amarine sponge-derived Streptomyces sp. SpD081030ME-02. J Antibiot. 63, 267-269 (2010)
62. Ueda JY, Hashimoto J, Inaba S, Takagi M* and Shin-ya K. JBIR-59, a new sorbicillinoid, from amarine-derived fungus Penicillium citrinum SpI080624G1f01. J.Antibiot. 63, 203-205 (2010)
63. Baumann S, Schoof S, Bolten M, Haering C, Takagi M, Shin-ya K and Arndt HD. Molecular determinants of
microbial resistance to thiopeptide antibiotics. J Am Chem Soc. 132, 6973-6981 (2010)
64. Takagi M,* Motohashi K, Hwang JH, Nagai A and Shin-ya K. New tensidols, JBIR-86 and JBIR-87, isolated
from Aspergillus sp. fJ80. J Antibiot. 63, 371-373 (2010)
65. Tanaka H, Yamaguchi S, Yoshizawa A, Takagi M, Shin-ya K and Takahashi T. Combinatorial synthesis of
deoxyhexasaccharides related to the landomycin A sugar moiety, based on an orthogonal deprotection strategy.
Chem Asian J. 5, 1407-1427 (2010)
66. Khan ST, Takagi M* and Shin-ya K. Diversity, salt requirement, and antibiotic production of Actinobacteria
isolated from marine sponges. Actinomycetologica. 24, 18-23 (2010)
67. Hayakawa Y, Yamazaki Y, Kurita M, Kawasaki T, Takagi M and Shin-ya K. Flaviogeranin, a newneuroprotective compound from Streptomyces sp. J Antibiot. 63, 379-380 (2010)
68. Izumikawa M, Hashimoto J, Takagi M* and Shin-ya K. Isolation of two new terpeptin analogs-JBIR-81 andJBIR-82-from a seaweed-derived fungus, Aspergillus sp. SpD081030G1f1. J Antibiot. 63, 389-391 (2010)
69. Takagi M*, Motohashi K and Shin-ya K. Isolation of 2 new metabolites, JBIR-74 and JBIR-75, from thesponge-derived Aspergillus sp. fS14. J Antibiot. 63, 393-395 (2010)
70. Takagi M*, Motohashi K, Khan ST, Hashimoto J and Shin-ya K. JBIR-65, a new diterpene, isolated from asponge-derived Actinomadura sp. SpB081030SC-15. J Antibiot. 63, 401-403 (2010)7

71. Takagi M,* Motohashi K, Nagai A, Hashimoto J and Shin-ya K. JBIR-83 and JBIR-84, new promothiocin
derivatives, isolated from Streptomyces sp. RI19. J Antibiot. 63, 405-408 (2010)
72. Khan ST, Tamura T, Takagi M and Shin-ya K. Streptomyces tateyamensis sp. nov., Streptomyces marinus sp.
nov. and Streptomyces haliclonae sp. nov., three novel species of Streptomyces isolated from marine sponge
Haliclona sp. Int J Syst Evol Microbiol.60, 2775-2779 (2010)
73. Motohashi K, Toda T, Sue M, Furihata K, Shizuri Y, Matsuo Y, Kasai H, Shin-ya K, Takagi M, Izumikawa M,
Horikawa Y and Seto H. Isolation and structure elucidation of tumescenamides A and B, two peptides producedby Streptomyces tumescens YM23-260. J Antibiot.63, 549-552 (2010)
74. Motohashi K, Takagi M,* Yamamura H, Hayakawa M and Shin-ya K. A new angucycline and a new
butenolide isolated from lichen-derived Streptomyces spp. J Antibiot. 63, 545-548 (2010)
75. Ueda JY, Takagi M* and Shin-ya K. New xanthoquinodin-like compounds, JBIR-97, -98 and -99, obtained
from marine sponge-derived fungus Tritirachium sp. SpB081112MEf2. J Antibiot. 63, 615-618 (2010)
76. Yoshida M, Takeuchi H, Ishida Y, Yashiroda Y, Yoshida M, Takagi M, Shin-ya K and Doi T. Synthesis,
structure determination, and biological evaluation of destruxin E. Org Lett. 12, 3792-3795 (2010)
77. Ueda JY, Hashimoto J, Yamamura H, Hayakawa M, Takagi M* and Shin-ya K. A new 16-membered tetraene
macrolide JBIR-100 from a newly identified Streptomyces species. J. Antibiot. 63, 627-629 (2010)
78. Takagi M,* Motohashi K, Nagai A, Izumikawa M, Tanaka M, Fuse S, Doi T, Iwase K, Kawaguchi A, Nagata
K, Takahashi T and Shin-ya K. Anti-influenza virus compound from Streptomyces sp. RI18. Org Lett. 12,4664-4666 (2010)
79. Takagi M,* Motohashi K, Izumikawa M, Khan ST, Hwang JH and Shin-ya K. JBIR-66, a new metabolite
isolated from tunicate-derived Saccharopolyspora sp. SS081219JE-28. Biosci Biotechnol Biochem. 74,
2355-2357 (2010)
80. Izumikawa M, Nagai A, Hashimoto J, Takagi M* and Shin-ya K. Isolation of 2 new naphthablin analogs,
JBIR-79 and JBIR-80, from Streptomyces sp. RI24. J Antibiot 63, 729-731 (2010)
81. Kunoh T, Noda T, Koseki K, Sekigawa M, Takagi M, Shin-ya K, Goshima N, Iemura S, Natsume T, Wada S,

8Mukai Y, Ohta S, Sasaki R and Mizukami T. A novel human dynactin-associated protein, dynAP, promotes
activation of Akt, and ergosterol-related compounds induce dynAP-dependent apoptosis of human cancer cells.
Mol Cancer Ther. 9, 2934-2942 (2010)
82. Komaki H, Ando K, Takagi M* and Shin-ya K. Dereplication of Streptomyces strains by automated southernhybridization with a polyketide synthase gene probe. Actinomycetologica. 24, 66-69 (2010)
83. Hwang JH, Takagi M,* Murakami H, Sekido Y and Shin-ya K. Induction of tubulin polymerization andapoptosis in malignant mesothelioma cells by a new compound JBIR-23. Cancer Lett. 300, 189-196 (2011)84. Doi T, Shibata K, Yoshida M, Takagi M, Tera M, Nagasawa K, Shin-ya K and Takahashi T. (S)-Stereoisomerof telomestatin as a potent G-quadruplex binder and telomerase inhibitor. Org Biomol Chem. 9, 387-393 (2011)85. Doi T, Numajiri Y, Takahashi T, Takagi M and Shin-ya K. Solid-phase total synthesis of (-)-apratoxin A and
its analogues and their biological evaluation. Chem Asian J. 6, 180-188 (2011)
86. Khan ST, Komaki H, Motohashi K, Kozone I, Mukai A, Takagi M* and Shin-ya K. Streptomyces associatedwith a marine sponge Haliclona sp.; biosynthetic genes for secondary metabolites and products. Environ
Microbiol. 13, 391-403 (2011)
87. Nagai A, Khan ST, Tamura T, Takagi M and Shin-ya K. Streptomyces aomiensis sp. nov., a novel species ofStreptomyces isolated from a soil sample using membrane filter method. Int J Syst Evol Microbiol. 61, 947-950(2011)
88. Motohashi K, Nagai A, Takagi M* and Shin-ya K. Two novel benzastatin derivatives, JBIR-67 and JBIR-73,
isolated from Streptomyces sp. RI18. J Antibiot. 64, 281-283 (2011)
89. Kozone I, Izumikawa M, Motohashi K, Nagai A, Yoshida M, Doi T, Takagi M* and Shin-ya K. Isolation of
new hexapeptides—JBIR-39 and JBIR-40—from a marine sponge-derived Streptomyces sp. Sp080513SC-24. J
Marine Sci Res Development. 1, 1000101 (2011)
90. Ueda JY, Izumikawa M, Mukai A, Nagai A, Hwang JH, Takagi M* and Shin-ya K. New angucycline
C-glycosides from Streptomyces sp. RI33. J Antibiot. 64, 367-372 (2011)
91. Fuse S, Okada K, Iijima Y, Munakata A, Machida K, Takahashi T, Takagi M, Shin-ya K, and Doi T. Total

9synthesis of spiruchostatin B aided by an automated synthesizer. Org Biomol Chem. 9, 3825-3833 (2011)
92. Ueda JY, Izumikawa M, Kozone I, Yamamura H, Hayakawa M, Takagi M* and Shin-ya K. A
phenylacetylated peptide, JBIR-96, isolated from Streptomyces sp. RI051-SDHV6. J Nat Prod. 74, 1344-1347(2011)
93. Motohashi K, Inaba K, Fuse S, Doi T, Izumikawa M, Khan ST, Takagi M,* Takahashi T and Shin-ya K.
JBIR-56 and JBIR-57, 2(1H)-pyrazinones from a marine sponge-derived Streptomyces sp. SpD081030SC-03. J
Nat Prod. 74, 1630-1635 (2011)
94. Takagi M* and Shin-ya K. New species of actinomycetes do not always produce new compounds with highfrequency. J Antibiot. 64, 699-701 (2011)95. Izumikawa M, Takagi M* and Shin-ya K. Isolation of a novel macrocyclic dilactone - JBIR-101 - from
Promicromonospora sp. RL26. J Antibiot. 64, 689-691 (2011)
96. Izumikawa M, Satou R, Motohashi K, Nagai A, Ohnishi Y, Takagi M,* Shin-ya K. Naphthoquinone-likepolyketide isolated from Streptomyces sp. RI-77 and its predicted biosynthetic pathway. J Nat Prod. 74,
2588-2591 (2011)
97. Kawahara T, Takagi M* and Shin-ya K. JBIR-124: a novel antioxidative agent from a marine sponge-derived
fungus Penicillium citrinum SpI080624G1f01. J Antibiot. 65, 45-47 (2012)
98. Izumikawa M, Hosoya T, Takagi M* and Shin-ya K. A new cyclizidine analog-JBIR-102-from
Saccharopolyspora sp. RL78 isolated from mangrove soil. J Antibiot. 65, 41-43 (2012)
99. Kawahara T, Izumikawa M, Otoguro M, Yamamura H, Hayakawa M, Takagi M* and Shin-ya K. JBIR-94
and JBIR-125, antioxidative phenolic compounds from Streptomyces sp. R56-07. J Nat Prod. 75, 107-110 (2012)100. Izumikawa M, Takagi M* and Shin-ya K. JBIR-78 and JBIR-95: Phenylacetylated peptides isolated fromKibdelosporangium sp. AK-AA56. J Nat Prod. 75, 280-284 (2012)
101. Hosoya T, Hirokawa T, Takagi M* and Shin-ya K. Trichostatin Analogues JBIR-109, JBIR-110, and
JBIR-111 from the marine sponge-derived Streptomyces sp. RM72. J Nat Prod. 75, 285-289 (2012)10

102. Ohnuki S, Kobayashi T, Ogawa H, Kozone I, Ueda JY, Takagi M, Shin-Ya K, Hirata D, Nogami S and Ohya
Y. Analysis of the biological activity of a novel 24-membered macrolide JBIR-19 in Saccharomyces cerevisiaeby the morphological imaging program CalMorph. FEMS Yeast Res. 12, 293-304 (2012)
103. Khan ST, Takagi M and Shin-ya K. Actinobacteria associated with the marine sponges Cinachyra sp.,Petrosia sp., and Ulosa sp. and their culturability. Microbes Environ. 27, 99-104 (2012)
104. Kawahara T, Takagi M* and Shin-ya K. Three new depsipeptides, JBIR-113, JBIR-114 and JBIR-115,
isolated from a marine sponge-derived Penicillium sp. fS36. J Antibiot. 65, 147-150 (2012)
105. Takahashi M, Takemoto Y, Shimazu T, Kawasaki H, Tachibana M, Shinkai Y, Takagi M, Shin-ya K, Igarashi
Y, Ito A and Yoshida M. Inhibition of histone H3K9 methyltransferases by gliotoxin and related
epipolythiodioxopiperazines. J Antibiot. 65, 263-265 (2012)
106. Takagi M,* Ueda JY, Hwang JH, Hashimoto J, Izumikawa M, Murakami H, Sekido Y and Shin-ya K.
Tyrosyl-DNA phosphodiesterase 1 inhibitor from an anamorphic fungus. J Nat Prod. 75, 764-767 (2012)107. Kawahara T, Hosoya T, Tsukamoto M, Okabe S, Yamamura H, Hayakawa M, Seimiya H, Takagi M* andShin-ya K. JBIR-120: a new growth inhibitor of hormone-refractory prostate cancer cells. J Antibiot. 65,
373-375 (2012)108. Maruyama C, Toyoda J, Kato Y, Izumikawa M, Takagi M, Shin-Ya K, Katano H, Utagawa T, Hamano Y. A
stand-alone adenylation domain forms amide bonds in streptothricin biosynthesis. Nat Chem Biol. 8, 791-797(2012)
109. Kawahara T, Nagai A, Takagi M, Shin-ya K. JBIR-137 and JBIR-138, new secondary metabolites from
Aspergillus sp. fA75. J Antibiot. 65, 535-538 (2012)110. Kawahara T, Izumikawa M, Takagi M, Shin-ya K. Relative configuration of JBIR-129, a cytotoxic
34-membered glycosidic polyol macrolide from Streptomyces sp. RK74. Org Lett. 14, 4434-4437 (2012)111. Kawahara T, Nagai A, Takagi M, Shin-ya K. A new furaquinocin derivative, JBIR-136, from Streptomyces
sp. 4963H2. J Antibiot. 65, 579-581 (2012)112. Kawahara T, Hwang JH, Izumikawa M, Hashimoto J, Takagi M, Shin-ya K. JBIR-129 and -139, Cytotoxic

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34-membered polyol macrolides of microbial origin. J Nat Prod. 75, 1814−1818 (2012)113. Hosoya T, Takagi M, Shin-ya K. New pyridone alkaloids JBIR-130, JBIR-131 and JBIR-132 from Isaria sp.
NBRC 104353. J Antibiot. 66, 235-238 (2013)114. Izumikawa M, Kawahara T, Hwang JH, Takagi M, Shin-ya K. JBIR-107, a new metabolite from themarine-sponge-derived actinomycete, Streptomyces tateyamensis NBRC 105047. Biosci Biotechnol Biochem. 77,
663-665 (2013)115. Komatsu M, Komatsu K, Koiwai H, Yamada Y, Kozone I, Izumikawa M, Hashimoto J, Takagi M, Omura S,
Shin-ya K, Cane DE, Ikeda H. Engineered Streptomyces avermitilis Host for Heterologous Expression of
Biosynthetic Gene Cluster for Secondary Metabolites. ACS Synth Biol. 2, 384-396 (2013)116. Fuse S, Inaba K, Takagi M, Tanaka M, Hirokawa T, Johmoto K, Uekusa H, Shin-ya K, Takahashi T, Doi T.
Design and synthesis of 2-phenyl-1,4-dioxa-spiro[4.5]deca-6,9-dien-8-ones as potential anticancer agents
starting from cytotoxic spiromamakone A. Eur J Med Chem. 66, 180-184 (2013)
117. Hirohama M, Kumar A, Fukuda I, Matsuoka S, Igarashi Y, Saitoh H, Takagi M, Shin-ya K, Honda K,
Kondoh Y, Saito T, Nakao Y, Osada H, Zhang KY, Yoshida M, Ito A. Spectomycin B1 as a Novel SUMOylation
Inhibitor That Directly Binds to SUMO E2. ACS Chem Biol. 8, 2635-2642 (2013)
118. Shibata K, Yoshida M, Takahashi T, Takagi M, Shin-ya K, Doi T. Synthesis of hepta-oxazole macrocyclic
analogues of telomestatin and evaluation of their telomerase inhibitory activities. Bull. Chem. Soc. Jpn 86,1453-1465 (2013)119. Satou R, Izumikawa M, Katsuyama Y, Matsui M, Takagi M, Shin-ya K, Ohnishi Y. Isolation, structural
elucidation and biosynthesis of 3-hydroxy-6-dimethylallylindolin-2-one, a novel prenylated indole derivativefrom Actinoplanes missouriensis. J Antibiot. 67, 231-236 (2014)120. Doi T, Yoshida M, Ohsawa K, Shin-ya K, Takagi M, Uekusa Y, Yamaguchi T, Kato K, Hirokawa T,
Natsume T. Total synthesis and characterization of thielocin B1 as a protein–protein interaction inhibitor of
PAC3 homodimer. Chem Sci. 5, 1860-1868 (2014)121. Muliandi A, Katsuyama Y, Sone K, Izumikawa M, Moriya T, Hashimoto J, Kozone I, Takagi M, Shin-ya K,
Ohnishi Y. Biosynthesis of JBIR-34. Biosynthesis of the 4-methyloxazoline-containing nonribosomal peptides,

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JBIR-34 and -35, in Streptomyces sp. Sp080513GE-23. Chem Biol. 21, 923-934 (2015)122. Fujiwara K, Tsukamoto H, Izumikawa M, Hosoya T, Kagaya N, Takagi M, Yamamura H, Hayakawa M,
Shin-ya

K,

Doi

T.

Total

synthesis

andstructure

determinationof

JBIR-108-A

2-Hydroxy-2-(1-hydroxyethyl)-2,3-dihydro-3(2H)-furanone Isolated from Streptomyces gramineus IR087Pi-4. J
Org Chem. 80, 114-132 (2015)
123. Yoshida M, Sekioka N, Izumikawa M, Kozone I, Takagi M, Shin-ya K, Doi T. Total synthesis and structure
elucidation of JBIR-39: a linear hexapeptide possessing piperazic acid and γ-hydroxypiperazic acid residues.
Chemistry. 21, 3031-3041 (2015)
124. Izumikawa M, Kozone I, Hashimoto J, Kagaya N, Takagi M, Koiwai H, Komatsu M, Fujie M, Satoh N,
Ikeda H, Shin-ya K. Novel thioviridamide derivative--JBIR-140: heterologous expression of the gene cluster forthioviridamide biosynthesis. J Antibiot. 68, 533-536 (2015)125. Ohno S, Katsuyama Y, Tajima Y, Izumikawa M, Takagi M, Fujie M, Satoh N, Shin-ya K, Ohnishi Y.
Identification and Characterization of the Streptazone E Biosynthetic Gene Cluster in Streptomyces sp.
MSC090213JE08. Chembiochem. 16, 2385-2391 (2015)
126. Izumikawa M, Kawahara T, Kagaya N, Yamamura H, Hayakawa M, Takagi M, Yoshida M, Doi T, Shin-ya
K. Pyrrolidine-containing peptides, JBIR-126, -148, and -149, from Streptomyces sp. NBRC 111228.
Tetrahedron Lett. 56, 5333–5336 (2015)
127．Ishigamia K, Kobayashia M, Takagi M, Shin-ya K, Watanabea H. Synthesis of marine oxylipin topsentolide
A1 and its stereoisomers, and determination of the absolute configuration of the natural product. Tetrahedron 71,
8436–8443 (2015)
128. Motohashi K, Izumikawa M, Kagaya N, Takagi M, Shin-ya K. JBIR-76 and JBIR-77, modified
naphthoquinones from Streptomyces sp. RI-77. J Antibiot. (2016)
128. Higa A, Hoshi H, Takagi M*. Differing responses of human stem cell-derived cardiomyocytes toarrhythmogenic drugs, determined using impedance measurements. Fundam. Toxicol. Sci. 3, 47-53 (2016)129. Katsuyama Y, Sone K, Satou R, Izumikawa M, Takagi M, Fujie M, Satoh N, Shin-ya K, Ohnishi Y.
Involvement of the Baeyer-Villiger Monooxygenase IfnQ in the Biosynthesis of Isofuranonaphthoquinone
Scaffold of JBIR-76 and -77. Chembiochem. 17, 1021-1028 (2016)13

130. Hoshi H, Hiyama G, Ishikawa K, Inageda K, Fujimoto J, Wakamatsu A, Togashi T, Kawamura Y, Takahashi
N, Higa A, Goshima N, Semba K, Watanabe S, Takagi M*. Construction of a novel cell-based assay for theevaluation of anti-EGFR drug efficacy against EGFR mutation. Oncol Rep. 37, 66-76 (2017)131. Ito S, Ogawa K, Takeuchi K, Takagi M, Yoshida M, Hirokawa T, Hirayama S, Shin-ya K, Shimada I, Doi T,
Goshima N, Natsume T, Nagata K. A small-molecule compound inhibits a collagen-specific molecular chaperone
and could represent a potential remedy for fibrosis. J Biol Chem., 292, 20076-20085. (2017)132. Higa A, Hoshi H, Yanagisawa Y, Ito E, Morisawa G, Imai JI, Watanabe S, Takagi M*. Evaluation system forarrhythmogenic potential of drugs using human-induced pluripotent stem cell-derived cardiomyocytes and gene
expression analysis. J Toxicol Sci., 42, 755-761. (2017)133. Tsutsumi H, Katsuyama Y, Izumikawa M, Takagi M, Fujie M, Satoh N, Shin-Ya K, Ohnishi Y.
Unprecedented cyclization catalyzed by a cytochrome P450 in benzastatin biosynthesis. J Am Chem Soc., 40,
6631-6639 (2018).
134. Tamura H, Higa A, Hoshi H, Hiyama G, Takahashi N, Ryufuku M, Morisawa G, Yanagisawa Y, Ito E, ImaiJI, Dobashi Y, Katahira K, Soeda S, Watanabe T, Fujimori K, Watanabe S, Takagi M*. Evaluation of anticancer
agents using patient-derived tumor organoids characteristically similar to source tissues. Oncol Rep., 40,
635-646, (2018)135. Ohsawa K, Yoshida M, Izumikawa M, Takagi M, Shin-Ya K, Goshima N, Hirokawa T, Natsume T, Doi T.
Synthesis and biological evaluation of thielocin B1 analogues as protein-protein interaction inhibitors of PAC3
homodimer. Bioorg Med Chem. 26, 6023-6034, (2018)
136. Takahashi N, Hoshi H, Higa A, Hiyama G, Tamura H, Ogawa M, Takagi K, Goda K, Okabe N, Muto S,
Suzuki H, Shimomura K, Watanabe S, Takagi M. An in vitro system for evaluating molecular targeted drugs
using lung patient-derived tumor organoids. Cells. 8, 481 (2019)137. Takahashi N, Higa A, Hiyama G, Tamura H, Hoshi H, Dobashi Y, Katahira K, Ishihara H, Takagi K, Goda K,
Okabe N, Muto S, Suzuki H, Shimomura K, Watanabe S, Takagi M. Construction of in vitro patient-derived
tumor models to evaluate anticancer agents and cancer immunotherapy. Oncol Lett. 5, 406 (2021)
138. Higa A, Takahashi N, Hiyama G, Tamura H, Hoshi H, Shimomura K, Watanabe S, Takagi M.

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High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids. J Vis Exp. 172 (2021)
139. Sasaki K, Suzuki M, Sonoda T, Schneider-Poetsch T, Ito A, Takagi M, Fujishiro S, Sohtome Y, Dodo K,
Umehara T, Aburatani H, Shin-Ya K, Nakao Y, Sodeoka M, Yoshida M. Visualization of the dynamic interaction
between nucleosomal histone H3K9 tri-methylation and HP1α chromodomain in living cells. Cell Chem Biol. 29,
1153-1161.e5. (2022)
140. Ishikawa K, Tamamura S, Takahashi N, Takagi M, Semba K, Watanabe S. Isolation of Reporter Cells That
Respond to Vitamin A and/or D Using a piggyBac Transposon Promoter-Trapping Vector System. Int J Mol Sci.
23, 9366. (2022)*は、corresponding authorを示す。

総説

葛山智久、高木基樹、瀬戸治男
イソプレノイド生合成研究の新展開：有用イソプレノイドの生産を目指して
バイオサイエンスとインダストリー、2001. 59: 455-458

高木基樹、瀬戸治男、葛山智久
イソプレンユニット生合成の多様性
蛋白質 核酸 酵素、2002. 47: 58-65
Takagi M,* Shin-ya K. Construction of a natural product library containing secondary metabolites produced byactinomycetes. J Antibiot. 65, 443-447 (2012)高木 基樹、新家 一男
海洋由来の放線菌が生産する二次代謝産物の新規薬剤探索用ライブラリーの構築
バイオサイエンスとインダストリー、2014. 72: 208-212

高木基樹
新型コロナウイルスに対す抗体を活用したマスクの開発
光が丘(福島県立医科大学医学部同窓会報)、2022. 51: 70-71

15

高木基樹
新型コロナウイルスに対す抗体を活用した薬や衛生用品の開発
福島の進路、2022. 5: 5-8

高木基樹
がん組織に近い培養細胞の開発 世界へ提供可能に
光が丘(福島県立医科大学医学部同窓会報) 2023. 52: 74-75.

高木基樹
新型コロナウイルスに対す抗体を活用した薬や衛生用品の開発
日本大学知財ジャーナル、2023. 16: in press.

著書
福島PDO®を用いた抗がん剤の評価、比嘉亜里沙、高木基樹、実験医学別冊「患者由来がんモデルを用いたがん研究実践ガイド CDX・スフェロイド・オルガノイド・PDX/PDOX を網羅 臨床検体の取り扱い指針から樹立プロトコールと入手法まで」、佐々木博巳/編、羊土社、東京、2019年10月

RNAi 療法：siRNA のがん治療薬への応用、高木基樹、嶋本顕、古市泰宏、RNAi 法とアンチセンス法、(株)講談社、東京、2005 年 6 月
RecQヘリカーゼを標的とした制癌剤治療、高木基樹、佐藤あゆみ、嶋本顕、古市泰宏、遺伝子医学MOOK「RNAと創薬」、株式会社メディカル ドゥ、大阪、2006年1月

特許
1. 放線菌由来のメバロン酸経路に関与する酵素の遺伝子、発明者：瀬戸治男 葛山智久 高橋俊二 高木
基樹、特願平11‐348375、国際出願 PCT/JP2000/008620
2. 癌細胞に対するアポトーシス誘導剤、発明者：高木基樹、嶋本顕、古市泰宏、佐藤あゆみ、特願
2003-140685、国際出願 PCT/JP2004/007145
3. アルキル化薬感受性を上昇させる方法、発明者：高木基樹、嶋本顕、古市泰宏、特願2003-197918、
国際出願 PCT/JP2004/010445

16

4. 染色体安定化に関する遺伝子を標的とする癌細胞特異的アポトーシス誘導剤、発明者：高木基樹、
二見和伸、嶋本顕、古市泰宏、特願2004-115404、国際出願 PCT/JP2005/006914
5. 癌細胞特異的細胞増殖抑制剤、発明者：高木基樹、嶋本顕、古市泰宏、佐藤あゆみ、特願2004-336742、
国際出願 PCT/JP2005/021099
6. siRNA含有組成物、発明者：佐藤あゆみ、平井美和、高木基樹、嶋本顕、丸山厚、チェソンウォン、
狩野有宏、特願2005－142420、国際出願 PCT/JP2006/309699
7. 抗癌剤増感剤、発明者：二見和伸、高木基樹、杉本正信、古市泰宏、特願2006-265988、国際出願
PCT/JP2007/068980

8. 肝指向性リポソーム組成物、発明者：佐藤あゆみ、高木基樹、嶋本顕、川上 茂、橋田 充、特許出願2005-308288

9. テロメラーゼ阻害剤、発明者：長澤 和夫、寺 正行、新家 一男、高木 基樹、特願2008－166494、
国際出願 PCT/JP2009/061569

10. 薬剤の心毒性評価方法及びそのための試薬又はキット、発明者：高木 基樹、他、特願2017-141689

その他、8件を出願。

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