March 21st, 2024
PeptiGrowth Inc.

PeptiGrowth Inc. is Launching a Novel Synthetic Peptide
TPO-alternative peptide (TPOR agonist)
PeptiGrowth Inc. (Headquarters: Chiyoda-ku, Tokyo, President: Jiro Sugimoto) has successfully
developed a novel synthetic peptide called “TPO alternative peptide (TPOR agonist)” which has
equivalent function to recombinant TPO (Thrombopoietin). This product will be on the market at the endof March 2024.

Development of Synthetic Peptide Growth Factors by PeptiGrowth
Conventional growth factors and cytokines used in the manufacturing of regenerative medicine and celltherapy products face various quality challenges such as lot-to-lot variation, potential contamination withbiological impurities, low stability, and high cost. PeptiGrowth has been working on the development ofa series of synthetic peptides that can address these challenges while maintaining equivalent function to
the conventional growth factors and cytokines on the market. Our peptides are completely chemicallysynthesized and animal component-free, enabling Xeno-free and chemically defined cell culture media.

About TPO alternative peptide (TPOR agonist) [Product code: PG-010]➢ Mechanism of activating thrombopoietin receptor (TPOR) by the TPO alternative peptide (PG-010)
The TPO alternative peptide (PG-010) is a dimer peptide composed of cyclic peptides thatbind to thrombopoietin receptor (TPOR). Similar to TPO (thrombopoietin), it induces
dimerization of TPOR and demonstrates agonistic activity towards the TPO signaling pathway
through activation of JAK2 (Janus Kinase 2) (see diagram below).

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TPO signaling pathway activation is similar to recombinant TPO

We conducted a comparative study of the functions of TPO alternative peptide (PG-010) andcommercially available recombinant TPO. We confirmed that the TPO alternative peptide has the ability
to activate ERK, located downstream of the TPO signaling pathway similar to recombinant TPO (upperleft figure). Furthermore, we evaluated activation of the JAK2-STAT5 signaling pathway using a reporter
assay, which showed that the TPO alternative peptide can activate the JAK2-STAT5 signaling pathway
similar to TPO (upper right figure). Additionally, as a functional evaluation against human hematopoietic
stem cells (HSCs), the proliferative ability and undifferentiated maintenance ability of CD34 + cells wereevaluated. The results confirmed that the TPO alternative peptide has similar proliferative activity andundifferentiated maintenance ability for CD34+ cells as TPO.
We anticipate that the TPO alternative peptide (PG-010) can be utilized in regenerative medicine andcell therapy fields, particularly in applications such as proliferation and maturation of megakaryocytes forplatelet production, differentiation of HSCs from pluripotent stem cells, proliferation of HSCs, andmanufacturing of allogeneic CAR-T cells.

➢Product Overview

Product name:

TPO alternative peptide (TPOR agonist)

Product code:

PG-010

Product form:

Lyophilized

Storage conditions:-20°C or lessPurity:≥95% by HPLC

Molecular weight:

7148.02 (Acetate)Size:

25 μg (volume per glass vial)*This product can be provided with an Animal Component Free (ACF) certificate.
*This product is for testing and research use only (RUO).
*There is a possibility that the specifications can change.

2➢Purchase Our Product
If you would like to purchase our products, please contact us using the information below.

For customers who would like to purchase GMP compliant products as well as the above PG products inbulk scale, please contact us using the information below.

Development of other peptides from PeptiGrowth
We have developed alternative peptides targeting numerous growth factors and cytokines. To date, wehave launched 10 products and plan to launch several additional growth factor alternative peptides
targeting PDGF-AA, FGF2, KGF, etc. from the 2nd quarter of 2024 to the 1st half of 2025. Details will be
updated on our website. If you are interested, please contact us using the following contact information.

Contact Information
PeptiGrowth Inc.
Tel : + 81-(0)70-4503-1497
E-mail: contact@peptigrowth.com

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