࣭ၛఱ‫૽༹ڠ‬ȁఱिఱ‫ڠ‬๷୆໤ພࡄ‫ݪ‬ਫ਼

ĳıĲĳȽĳıĲĴ

࿒ষ

Contents‫ٽ͂ڟ״‬ါ

History & Outline

Ĳ

‫ࢹܥ‬

OrganizationĳႤయਫ਼ಿȟႤయ‫ޗ‬਎Former Directors & Professors

Ĵ

࿨૖֥൝

Department Heads

ĵࢹ଼֥

Faculty & StudentsĶࡄ‫ڰݪ‬൲͈‫ٽ‬ါResearch & Activitiesۜஅ‫ݪࡄࢹܥ‬໐࿝ȁȤȤȤȤȤȤȤȤȤȤȤȤȤȤȤȤ
୆ఘཡࢄࡄ‫ݪ‬໐࿝ȁȤȤȤȤȤȤȤȤȤȤȤȤȤȤȤȤ
۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝ȁȤȤȤȤȤȤȤȤȤȤȤȤȤȤȤȤ
ඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜȁȤȤȤȤȤȤȤȤȤȤȤ
֒ഥૂ༭৘ࡑΓϋΗȜȁȤȤȤȤȤȤȤȤȤȤȤȤȤȤ
ۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȁȤȤȤȤȤȤȤȤȤȤȤȤȤ

ۜஅછ‫ڠ‬྾‫ڠע‬ဏࣣίυΈρθଔૺ৒

ķ
Ĳĵ
ĳĵ
Ĵĳ
Ĵķ
ĵĳ

ĵĺ

Office of Combined Program on Microbiology and immunology඾ུȆΗͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜĶĲ

Thailand-Japan Research Collaboration Center on Emerging and Re-emerging InfectionsఱिġĮġζΪΡϋۜஅછΓϋΗȜĶĵMahidol-Osaka Center for Infectious Diseases

ΟϋΈχ·ΙϋȪिఱ๷୆໤ພࡄ‫ݪ‬ٛȫ‫ݪࡄ້ܙ‬໐࿝

ĶĶ

BIKEN Endowed Department of Dengue Vaccine Development

ΈυȜΨσńŐņίυΈρθ

Global COE ProgramĶķ້௺ࡄ‫ݪ‬ঔ୭ȟ‫ވ‬೒ࡄ‫ݪ‬ঔ୭

Research FacilitiesĶĺ྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜ

Immunology Frontier Research CenterķĶࠨॳȟົ౷ȟ࠺໤

Accounts & Building Area

ķĹ

մඤ଎

Map & Accessĸıນঞৢ૯ȁ࿤ോġฎ‫ٽ͂ڟ״‬ါȁHistory & Outline‫״‬‫ڟ‬ȁ઎გːාȪ1929ȫ൚শ͈ఱि֓‫ش‬ఱ‫ڠ‬Ȫ઎გ˒ාఱिೱ࣭ఱ‫ۯ֊ͅڠ‬ȫ‫ڠ‬ಿනུಿ२჊͂ळ޿ࠬୄ‫ޗڠ‬਎౓࢛ᦂඵ͉ȂఱिȂ૰࡫̦΋τρȆβΑΠ͈̈́̓‫ٸ‬ြഥஅພ͈૟ව࿝࡫̜̹̭͂̈́ͤ̾̾̽͂͂Ȃఱୃ 12 ා

͂
‫ٽ‬
ါ

͈‫۾‬൐ఱૼब͈‫̥ͣ߱ޗ‬Ȃ‫۾‬ୌȂඅͅఱिͅ๷୆໤ພͅ‫̳ͥ۾‬௙ࣣഎࡄ‫ͬ۾ܥݪ‬୭ၛ̳ͥຈါ଻ͬ୰̧Ȃ൚শ͈ఱिຸ౶মড়ന஝२჊͞ఱि़‫ފͅٮ‬ႁͬါ୏̱̹ȃ५࢛࡚൸ঙ͉Ȃ̭͈ါ୏ͅ؊̢̀ 20 ྔ‫̱ັܙͬ׫‬Ȃ̭ͦͤ͢ͅ઎გ˕ාȪ1934ȫˎ࠮Ȃఱिঌཤߊ൴ോୌ಴ˏ๔౷ͅࡄ‫ݪ‬ਫ਼ུ‫̹̱ࢥੋ̦܁‬ȃ൴ോ΅λϋΩᾼ͉Ȃ‫ͅܡ‬ಅ๶ࠫ‫ݪࡄړ‬ਫ਼Ȫಅ๶হֲ‫מ‬࿝ঙ͈‫ັܙ‬ȫ͂ఱिඅਂ๧ໂພࡄ‫ݪ‬ਫ਼Ȫඈ঎‫ັܙ͈ز‬ȫ̦̜ͤȂ‫ڰ‬อ̈́ࡄ‫ڰݪ‬൲࣐̞̹̦ͬ̽̀Ȃ̭ͦͣˎ‫ͬ۾ܥ‬໵̵̀Ȃ઎გ˕ාȪ1934ȫ˕࠮ 17 ඾ೂ႓ 270 ࣢ͤ͢ͅȂఱिೱ࣭ఱ‫້ڠ‬౾͈๷୆໤ພࡄ‫ݪ‬ਫ਼ۗଷ̦࢖ື̯ͦȂུࡄ‫ݪ‬ਫ਼̦อ௷̳ͥͅঢ̹̽ȃ̷͈ࢃ 30 ဒාͬࠐ̀Ȃࡄ‫ݪ‬໐࿝Ȃঔ୭͈௩‫ฺ̞ͅح‬൴ോ౷ߊ͉ষలͅ‫ޛ‬᳕̹͂̈́̽ȃ̹͘૧̱̞ࡄ‫ݪ‬‫ڰ‬൲ͅచ؊̱ංͥࡄ‫ݪ‬৒͂੨ঔ୭͈ା๵ਰ৘͈̹͛Ȃఱिఱ‫͈ڠ‬଑ന΅λϋΩΑൡْࣣ͈ࠗల֚ଈ̱͂̀Ȃ઎გ 42 ාȪ1967ȫ࡛ह౷ͅ֊ഢ̱̹ȃȁ໹଼ˑාȪ1993ȫ˔࠮֓‫ڠ‬໐້௺ພ͈֭଑ന΅λϋΩΑΰ͈֊ഢͬ‫ͅܥ‬Ȃ‫͈܈‬হၷȂࡄ‫́̓̈́ݪ‬ఉ̩͈৘ୡུ̧̜̬̹ͬ̀ࡄ‫ݪ‬ਫ਼້௺ພ͉֭֓‫ڠ‬໐້௺ພ֭͂ൡࣣȂࣣ໵̱Ȃ60 ා̹̥̱̞ͩͥܵͅႤঃ͈ྃͬ໾
̲̹ȃུࡄ‫ݪ‬ਫ਼͉́Ȃ̭ͦͬࠀ‫̱̀͂ܥ‬Ȃ๷୆໤ພȂ̦ͭ‫͍ݞ‬අ೰͈ඳহ৖ۛͅ‫ڠ̳ͥ۾‬ၑ‫̷͈͍ݞ‬؊ဥࡄ‫͈֚ݪ‬௄͈อജͬ଎ͤȂˎˍଲ‫ͬܮ‬ജབ̱̹૧̱̞ࡄ‫ݪ‬ఘଷͬ‫ږ‬ၛ̳͓̩Ȃ໹଼˒ාȪ1994ȫਲြ͈໐࿝ଷ
ͬഢ̱۟Ȃఱ໐࿝ଷ͈͒٨ழ࣐̹ͬ̽ȃ໹଼ 17 ාȪ2005ȫ͉ۜͅஅ‫ࢹܥ‬Ȃ୆ఘཡࢄȂ۪‫ޏ‬؊൞͈ 3 ໐࿝Ȫ15 ໦࿤ȫͅठ༎଼̳ͥ͂͂͜ͅȂඳহ଻ۜஅછ࣪໚͈̹͛ͅඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜȪ3 ໦࿤ȫ͂ۜஅછ࣭षࡄ
‫ݪ‬ΓϋΗȜȪ2 ໐࿝ 1 ৒ȫͬ૧୭ȃ̹͘ȂΊΦθૂ༭ٜଢ଼͈ࡄ‫ٳݪ‬อͬଔૺ̳ͥ֒ഥૂ༭৘ࡑΓϋΗȜȪ3 ໦࿤ȫͬ‫ڠ‬ඤ‫ވ‬൳‫ݪࡄ֗ޗ‬ঔ୭ུ̥ͣࡄ‫ݪ‬ਫ਼້௺ঔ୭̱͂̀ൡࣣȂ̯ͣͅȂ૧‫ޟ‬Ȇठ‫ۜޟ‬அછଷգ̫̹࣭࢜ͅषࡄ
‫ݶݪ‬തȶ඾ུȆΗͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜȷͬΗͼ࣭ၛထཡ‫מ‬୆ࡄ‫ݪ‬ਫ਼ඤͅ୭౾̱̹ȃ໹଼ 21 ාȪ2009ȫ
6 ࠮͉ͅȂ໲໐‫ڠش‬ఱ૳̥ͣ‫ވ‬൳၌ဥȆ‫ވ‬൳ࡄ‫ݶݪ‬ത̱͂̀෇೰ͬ਋̫Ȃ໹଼ 22 ාȪ2010ȫ4 ࠮̥ͣ‫ڰ‬൲ͬ‫ٳ‬ইȃ̯ͣͅȂ֒ഥঊ঩࡙͈‫ږ‬༗͂౶എਫ਼ခࡀ͈‫ږ‬༗ͬ࿒എ̱̹͂ȶ୆ఘ؊൞֒ഥঊٜଢ଼ΓϋΗȜȷͬ૧୭̱̹ȃȁུࡄ‫ݪ‬ਫ਼͉Ȃ஻ၛոြۜஅછ͈‫ܖ‬யഎࡄ‫̷͈͍ͣ̈́ͅݪ‬ଷգ̞̾̀ͅࡄ‫͛ૺͬݪ‬Ȃ૧̹̈́ພࡔ޿͞ພࡔ;ͼσΑ͈อࡉȂอພ͈ι΃ΣΒθ͈ٜྶȂχ·Ιϋ͞૷౯स͈‫ٳ‬อ̈́̓Ȃِ̦࣭͈ۜஅછ‫͍ݞ‬྾‫ڠע‬໦࿤́ఉఱ͈࣓ࡃ࣐ͬ̽̀ြ̹ȃ̹͘Ȃ̦ͭࡄ‫͈ݪ‬໦࿤̤̞̀͜ͅ઎გ 11 ාȪ1936ȫͅρΐ;θͬঀဥ̱̹ࡄ‫ٳͬݪ‬ই̳ͥ̈́̓Ȃఈ͈‫̦̫ͭ̀ߐ୶ͅ۾ܥ‬்͈‫ܢ‬อࡉ͂হၷ༹͈‫ٳ‬อ഼ͅႁ̳ͥ͂͂͜ͅȂ̦ͭอ୆͈ι΃ΣΒθ͈ࡄ‫ͬݪ‬ଔૺ̱̹ȃ̭͈࿂̤ͅ
̞̀͜Ȃଲ‫̹̫ߐ୶ͅٮ‬෽ူळ༾͈อ଼̦͈ͭࢗȂ̦ͭ֒ഥঊ̦ͭ͞;ͼσΑ͈อࡉ̈́̓ఉ଼̩͈‫ͬض‬̜̬Ȃ̦ͭࡄ‫͈ݪ‬อജͅఱ̧̩࣓ࡃ̱̹ȃ̹͘Ȃඳহ଻֒ഥঊ৖͈ۛࡄ‫̞̤֚̀͜ͅݪ‬໐ࡔ֦֒ഥঊ͈
๷୆໤ພࡄ‫ݪ‬ਫ਼୭ၛဇြൾโ‫ڣ‬Ȫུ‫۾࡚܁‬γȜσȫౙၗ̷͈͂‫ܥ‬ෝٜଢ଼̈́̓࿹̹ͦࡄ‫ૺ̦ݪ‬ജಎ̜́ͥȃ༷֚Ȃུࡄ‫ݪ‬ਫ਼́ड੝ͅอࡉ̯̹ͦळ༾ဏࣣ࡛
ય͉ఘळ༾֒ഥ‫͈ڠ‬อജ͞ౙ·υȜϋࢯఘ͈‫ٳ‬อ̈́५࢛࡚൸Ȫ̪̻̬̠ͭ̓͘͞ȫ࣓̓ͅࡃ̱Ȃ࡛య͈୆ྵ‫ܖ͈ڠش‬யͬಃ̞̹ȃĲĹķĴ ා֓঍͈ಿ౳̱͂̀๶ൽ́୆ͦͥ͘ȃĲĶ प́ఱिͅ੄̀Ȃ഼ႁ͈ྎ‫۾‬ୌͬయນ̳ͥ৘ު‫͂ز‬̈́ͥȃఱୃ˒ා͉ͅমު̥ͣ਀̧ͬ֨Ȃু఺́ಋൽ͂
૞ަ͈୆‫ͅڰ‬වͥȃু़͈ͣॲͬ࢖‫ވ‬মު͞২঳
͈͒‫́ࠁ̠̞͂ૺܙ‬২ٛ‫ࡓۺ‬ȃȁ̤̈́Ȃུࡄ‫ݪ‬ਫ਼͉́஠‫ڠུ̦֥ޗ‬ఱ‫ࡄࠏڠ֭֓ڠ‬‫شݪ‬Ȫ֚໐͉ၑ‫شݪࡄڠ‬Ȃ࿪‫شݪࡄڠ‬Ȇ୆ྵ‫ܥ‬ෝࡄ‫͜شݪ‬ȫฎআ‫ه‬೾͍̈́ͣͅਘআ‫ه‬೾͈‫ͬ֗ޗ‬౜൚̳ͥ͂‫଼̞̹̜͈ͥ̀̽֗ͅऺ૽ͅވ‬ȃ
Ĳ

‫ࢹܥ‬ȁOrganizationਫ਼ಿȁDirectorయ݈֥ٛȁDelegate Assembly‫ޗ‬਎ٛȁFaculty Meetingࡄ‫ݪ‬໐࿝ȁResearch Divisionۜஅ‫ݪࡄࢹܥ‬໐࿝ȁ໦ঊळ޿‫ڠ‬໦࿤ȁ;ͼσΑۜஅଷࢄ໦࿤
ȁ໦ঊ;ͼσΑ໦࿤ȁ࿪໤ၷ༹໦࿤
ȁۜஅພఠ໦࿤
୆ఘཡࢄࡄ‫ݪ‬໐࿝ȁ໦ঊ྾‫ע‬ଷࢄ໦࿤ȁ྾‫ע‬ະ஠৖ۛࡄ‫ݪ‬໦࿤ȁুட྾‫ڠע‬໦࿤ȁळ༾‫ܥ‬ෝ໦࿤ȁ྾‫ڠاע‬໦࿤۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝ȁ֒ഥঊ୆໤‫ڠ‬໦࿤ȁ໦ঊ֒ഥࡄ‫ݪ‬໦࿤ȁอ‫܈‬ଷࢄࡄ‫ݪ‬໦࿤ȁૂ༭ഥో໦࿤
ȁळ༾ଷࢄ໦࿤

ŅŪŷŪŴŪŰůġŰŧġŊůŧŦŤŵŪŰŶŴġŅŪŴŦŢŴŦŴ
ȁŅŦűŢųŵŮŦůŵġŰŧġŎŰŭŦŤŶŭŢųġŃŢŤŵŦųŪŰŭŰŨźġ
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ŅŪŷŪŴŪŰůġŰŧġŉŰŴŵġŅŦŧŦůŴŦ
ȁŅŦűŢųŵŮŦůŵġŰŧġŎŰŭŦŤŶŭŢųġŊŮŮŶůŰŭŰŨź
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ȁŅŦűŢųŵŮŦůŵġŰŧġŊŮŮŶůŰŤũŦŮŪŴŵųź
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ȁŅŦűŢųŵŮŦůŵġŰŧġńŦŭŭŶŭŢųġœŦŨŶŭŢŵŪŰů

້௺ঔ୭ȁSpecial Research Facilitiesඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜȁळ޿ۜஅ໦࿤
ȁ໦ঊࡔಖ‫ڠ‬໦࿤ȁ;ͼσΑ྾‫ע‬໦࿤֒ഥૂ༭৘ࡑΓϋΗȜȁ֒ഥঊ‫ܥ‬ෝٜଢ଼໦࿤
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ȁŅŦűŢųŵŮŦůŵġŰŧġŗŪųŰŭŰŨź
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łůŪŮŢŭġœŦŴŰŶųŤŦġńŦůŵŦųġŧŰųġŊůŧŦŤŵŪŰŶŴġŅŪŴŦŢŴŦŴ
ŅŏłĮŤũŪűġŅŦŷŦŭŰűŮŦůŵġńŦůŵŦųġŧŰųġŊůŧŦŤŵŪŰŶŴġŅŪŴŦŢŴŦŴ
ńŦůŵŦųġŧŰųġňŦůŦŵŪŤġłůŢŭźŴŪŴġŰŧġŃŪŰŭŰŨŪŤŢŭġœŦŴűŰůŴŦŴ

ۜஅછ‫ڠ‬྾‫ڠע‬ဏࣣίυΈρθଔૺ৒ȁOffice of Combined Program on Microbiology and immunologyࡄ‫ݪ‬ଔૺΈσȜί‫֗ޗ‬ଔૺΈσȜίœŦŴŦŢųŤũġőųŰŮŰŵŪŰůġňųŰŶű
ņťŶŤŢŵŪŰůġőųŰŮŰŵŪŰůġňųŰŶű

٬‫ݶݪࡄٸ‬തȁResearch Collaboration Center in Overseas඾ུȆΗͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜ ŕũŢŪŭŢůťĮŋŢűŢůġœŦŴŦŢųŤũġńŰŭŭŢţŰųŢŵŪŰůġńŦůŵŦųġŰůġņŮŦųŨŪůŨġŢůťġœŦĮŦŮŦųŨŪůŨġŊůŧŦŤŵŪŰůŴ
ȁळ޿ۜஅ໐࿝
ȁŔŦŤŵŪŰůġŰŧġŃŢŤŵŦųŪŢŭġŊůŧŦŤŵŪŰůŴ
ȁ;ͼσΑۜஅ໐࿝
ȁŔŦŤŵŪŰůġŰŧġŗŪųŢŭġŊůŧŦŤŵŪŰůŴ
ఱिȽζΪΡϋۜஅછΓϋΗȜŎŢũŪťŰŭĮŐŴŢŬŢġńŦůŵŦųġŧŰųġŊůŧŦŤŵŪŰŶŴġŅŪŴŦŢŴŦŴ
ΟϋΈχ·ΙϋȪिఱ๷୆໤ພࡄ‫ݪ‬ٛȫ‫ݪࡄ້ܙ‬໐࿝ġġġġġġġġġġġġġġġġġġġġġġġġġġġŃŊŌņŏġņůťŰŸŦťġŅŦűŢųŵŮŦůŵġŰŧġŅŦůŨŶŦġŗŢŤŤŪůŦġŅŦŷŦŭŰűŮŦůŵ

‫ވ‬೒ঔ୭ȁCommon Research Facilitiesಎ؇৘ࡑ৒༶ৣ଻൳պࡓள৘ࡑ৒
ۜஅછ‫ވ‬൳৘ࡑ৒଎੥৒ńŦůŵųŢŭġŊůŴŵųŶŮŦůŵŢŵŪŰůġōŢţŰųŢŵŰųź
œŢťŪŰŪŴŰŵŰűŦġōŢţŰųŢŵŰųź
ńŦůŵųŢŭġōŢţŰųŢŵŰųźġŧŰųġŃŪŰŭŰŨŪŤŢŭġŉŢŻŢųťŰŶŴġŎŪŤųŰţŦŴ
ōŪţųŢųź

মྩ໐ȁAdministration੢ྩ߸ٛࠗ߸ࡄ‫ފݪ‬ႁ߸

ňŦůŦųŢŭġłŧŧŢŪųŴġŔŦŤŵŪŰů
łŤŤŰŶůŵŪůŨġŔŦŤŵŪŰů
œŦŴŦŢųŤũġńŰŰűŦųŢŵŪŰůġŔŦŤŵŪŰů

ଲ‫ٮ‬ΠΛίτασ‫ݶ‬തȁWorld Premier International Research Center྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜĳŊŮŮŶůŰŭŰŨźġŇųŰůŵŪŦųġœŦŴŦŢųŤũġńŦůŵŦų

Ⴄయਫ਼ಿȟႤయ‫ޗ‬਎ȁFormer Directors & ProfessorsႤయਫ਼ಿȁFormer Directors੝యġ ࡣ໌ġ࿥অ჊ġȪ઎გ˕ා˕࠮ȡ઎გĲĶා˒࠮ȫˎయġ ࣽఆġ࣏౳ġ Ȫ઎გĲĶා˔࠮ȡ઎გĲĹා˓࠮ȫġˏయġ ౓࢛ġᦂඵġ Ȫ઎გĲĹා˓࠮ȡ઎გĴıාˏ࠮ȫːయġ ൥࿤ġࢭ२჊ġȪ઎გĴıාː࠮ȡ઎გĴĴාˏ࠮ȫˑయġ ‫ۀ‬൸ġਗ਼ఊ჊ġȪ઎გĴĴාː࠮ȡ઎გĴĺාˏ࠮ȫ˒యġ ഛ࿤ġࢭ‫ݛ‬ġ Ȫ઎გĴĺාː࠮ȡ઎გĵĴාˏ࠮ȫ˓యġ ؈࿤ġၻ૳ġ Ȫ઎გĵĴාː࠮ȡ઎გĵĸාˏ࠮ȫ˔యġ ཱġ२ೋຳġ Ȫ઎გĵĸාː࠮ȡ઎გĶĲාˏ࠮ȫ˕యġ ୼ྋġਜ਼֚ġ Ȫ઎გĶĲාː࠮ȡ઎გĶĶාˏ࠮ȫ
Ĳıయġ ‫ح‬൥ġঅ჊ġ Ȫ઎გĶĶාː࠮ȡ઎გĶĺාˏ࠮ȫ
ĲĲయġ ࣞ‫ޘ‬ġၑྶġ Ȫ઎გĶĺාː࠮ȡ઎გķĲාˏ࠮ȫĲĳయġ २ႊ౓ġੇຳġȪ઎გķĲාː࠮ȡ઎გķĴාˏ࠮ȫĲĴయġ ‫זڙ‬ġ໌ຳġ Ȫ઎გķĴාː࠮ȡ઎გķĴා˕࠮ȫĲĵయġ ൥๶ġ߼ġȪ઎გķĴාĲĲ࠮ȡ໹଼ˎාĲı࠮ȫĲĶయġ ཅോġ‫ݛ‬૯౳ġȪ໹଼ˎාĲĲ࠮ȡ໹଼ˑාĲı࠮ȫĲķయġ ֵாġྶġȪ໹଼ˑාĲı࠮ȡ໹଼˕ාĲı࠮ȫĲĸయġ ୌਕġ݅໌ġ Ȫ໹଼˕ාĲı࠮ȡ໹଼ĲĴාĲı࠮ȫĲĹయġ ུനġ໌ংġ Ȫ໹଼ĲĴාĲı࠮ȡ໹଼ĲĶාĲı࠮ȫĲĺయġ ࿐‫ئ‬ġΗυ;ġȪ໹଼ĲĶාĲı࠮ȡ໹଼ĲĺාĲı࠮ȫ
ĳıయġ ݊౓ġ૾ġȪ໹଼ĲĺාĲı࠮ȡ໹଼ĳĴාĲı࠮ȫ
ĳĲయġ ࿒‫ح‬നġ‫ם‬༟ġȪ໹଼ĳĴාĲı࠮ȡġġġġġġġġġġġġġġġġġġġȫ

ŚŢŴũŪųŰġŌŰŵŢŬŦĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺĴĵį˕ȽĲĺĵıįġķ
łųŢŰġŊŮŢŮŶųŢĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
Ĳĺĵıį˔ȽĲĺĵĴįġĸ
ŕŦůūŪġŕŢůŪŨŶŤũŪĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺĵĴį˓ȽĲĺĶĶįġĴ
ŕŴŶůŦŴŢţŶųŰġŇŶūŪůŰĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ ĲĺĶĶįːȽĲĺĶĹįġĴ
ŋŶůŵŢųŰġŌŢŮŢũŰųŢĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ ĲĺĶĹįːȽĲĺķĵįġĴ
ŕŴŶůŦũŪŴŢġłŮŢůŰĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ ĲĺķĵįːȽĲĺķĹįġĴ
ŚŰŴũŪŰŮŪġŐŬŶůŰĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺķĹįːȽĲĺĸĳįġĴ
ŎŪŵŴŶŰġŉŰųŪĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺĸĳįːȽĲĺĸķįġĴ
ŋŶůŪŤũŪġŌŢŸŢŮŢŵŢĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ ĲĺĸķįːȽĲĺĹıįġĴ
ŔũŪųŰġŌŢŵŰĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺĹıįːȽĲĺĹĵįġĴ
ŎŪŤũŪŢŬŪġŕŢŬŢũŢŴũŪĭŎįŅįĭġőųŰŧŦŴŴŰųġġ ĲĺĹĵįːȽĲĺĹķįġĴ
ŕŰŴũŪŰġŎŪŸŢŵŢůŪĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺĹķįːȽĲĺĹĹįġĴ
ŕŢŬŦŰġŌŢŬŶůŢŨŢĭġŅįőũŢųŮįĭġőųŰŧŦŴŴŰųġġ ĲĺĹĹįːȽĲĺĹĹįġĺ
ŉŢūŪŮŦġŇŶūŪŰĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĲĺĹĹįĲĲȽĲĺĺıįĲı
ŌŶŮŢŰġŕŰźŰŴũŪŮŢĭġŎįŅįĭġőųŰŧŦŴŴŰųġ ĲĺĺıįĲĲȽĲĺĺĴįĲı
łŬŪųŢġŉŢŬŶųŢĭġŅįŔŤįĭġőųŰŧŦŴŴŰųġġ
ĲĺĺĴįĲıȽĲĺĺĸįĲı
ŚŰŴũŪŵŢŬŦġŏŪŴũŪŮŶůŦĭŎįŅįĭġőųŰŧŦŴŴŰųġġĲĺĺĸįĲıȽĳııĲįĲı
ŕŢŬŦūŪġŉŰůťŢĭġŎįŅįĭġőųŰŧŦŴŴŰųġġ
ĳııĲįĲıȽĳııĴįĲı
ŕŢųŰũġŌŪůŰŴũŪŵŢĭġŅįŎŦťįŔŤįĭġőųŰŧŦŴŴŰųġġĳııĴįĲıȽĳııĸįĲı
ŉŪŵŰŴũŪġŌŪŬŶŵŢůŪĭġŎįŅįĭġőųŰŧŦŴŴŰųġ
ĳııĸįĲıȽĳıĲĲįĲı
ņŪŴŶŬŦġŎŦŬŢťŢĭġőũįŅįĭġőųŰŧŦŴŴŰųġ
ĳıĲĲįĲıȽ

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ȟ
Ⴄ
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ਫ਼
ಿ
ȟ
Ⴄ
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‫ޗ‬
਎

Ⴄయ‫ޗ‬਎ȁFormer Professors֓‫ڠ‬ฎআȁࡣ໌ġ࿥অ჊ġਫ਼ಿ֓‫ڠ‬ฎআȁ‫݌‬നġ೭ဎġ‫ܙ‬୆ಖ‫ڠ‬֓‫ڠ‬ฎാȁࣽఆġ࣏౳ġਫ਼ಿ֓‫ڠ‬ฎআȁऎ౓ġခ‫݌‬ġឦহၷࡄ‫ݪ‬໐֓‫ڠ‬ฎআȁ౓࢛ġᦂඵġਫ਼ಿ֓‫ڠ‬ฎআȁଲၻġࢡఊġळ޿‫ڠا‬໐֓‫ڠ‬ฎআȁଽ५ġၨඃġ‫܈‬হၷ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁఱ౓ġય໹ġळ޿‫ڠا‬໐֓‫ڠ‬ฎআȁ‫۾‬ġ೶অ჊ġཡ‫ڠע‬໐
֓‫ڠ‬ฎআȁଌനġୃྜġळ޿‫ڠا‬໐֓‫ڠ‬ฎআȁ૩‫ئ‬ġ߰ġ‫ܙ‬୆ಖࡔಖ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁ५࢛ġ਍ġႉ઄໐࿝֓‫ڠ‬ฎআȁ൥࿤ġࢭ२჊ġळ޿ࠬୄ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁչ൥ġଽ֚ġల֚ࠫ‫شݪࡄړ‬֓‫ڠ‬ฎআȁ‫ۀ‬൸ġਗ਼ఊ჊ġਆន;ͼσΑ໐࿝֓‫ڠ‬ฎআȁୌఆġ៍ඵġឦ໐࿝֓‫ڠ‬ฎআȁ‫ح‬൥ġ֢຃ġࠫ‫ړ‬ພၑ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁༀനġଽ຃ġࢯॸ޿୆ၑ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁঢ়ġ࿆ġႉ઄സ࿝֓‫ڠ‬ฎআȁಞ࿐ġୃ२ġ‫ܙ‬୆ಖࡔಖ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁཱġ२ೋຳġࠫ‫ړ‬ພၑ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁ؈࿤ġၻ૳ġཻ૮໐࿝֓‫ڠ‬ฎআȁ୞ષġਹ࣐ġႉ઄໐࿝֓‫ڠ‬ฎআȁഛ࿤ġࢭ‫ݛ‬ġ྾‫ڠاע‬໐࿝֓‫ڠ‬ฎআȁ୼ྋġਜ਼֚ġ‫ڠا‬ၷ༹໐࿝
֓‫ڠ‬ฎআȁ‫ؖ‬നġ஝ဎġ൲໤;ͼσΑ໐࿝ၑ‫ڠ‬ฎআȁು‫ݳ‬ġ࢕ဎġ྾‫ڠاע‬໐࿝֓‫ڠ‬ฎআȁ૬֔ġࢣ෗੩ġཡ‫ڠע‬໐࿝
֓‫ڠ‬ฎআȁ૩ġၨ౳ġࠫ‫ړ‬ພၑ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁչ൥ġ၌आఊ჊ġ ឦ໐࿝࿪‫ڠ‬ฎআȁ‫זڙ‬ġ໌ຳġอ‫֒܈‬ഥঊ໐࿝
֓‫ڠ‬ฎআȁ‫ح‬൥ġঅ჊ġۜஅພၑ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁಎႅġຮຳġࡔಖ‫ڠ‬໐࿝
֓‫ڠ‬ฎআȁ५෗ඤġࢣંġۜஅ൲໤৘ࡑঔ୭

֓‫ڠ‬ฎআȁ२ႊ౓ġੇຳġ ळ޿ࠬୄ‫ڠ‬໐࿝֓‫ڠ‬ฎআȁࣞ‫ޘ‬ġၑྶġ ཻ૮໐࿝֓‫ڠ‬ฎআȁ൥๶ġ߼ġ྾‫ڠاע‬໐࿝֓‫ڠ‬ฎআȁന࢛ġᲔ౳ġ ႉ઄໐࿝ၑ‫ڠ‬ฎআȁઐయġՔ२ġ ळ޿;ͼσΑ໐࿝ၑ‫ڠ‬ฎআȁಎനġඈ౳ġ ‫ڠا‬ၷ༹໐࿝֓‫ڠ‬ฎআȁ‫ؖ‬५ġฎ૽ġ ໦ঊ֒ഥࡄ‫ݪ‬໦࿤֓‫ڠ‬ฎআȁཅോġ‫ݛ‬૯౳ġ อ‫֒܈‬ഥঊ໐࿝֓‫ڠ‬ฎআȁ࿐౓ġચຳġ ႉ઄໐࿝֓‫ڠ‬ฎআȁࣞ֔ġ૧֚჊ġ ႉ઄໐࿝֓‫ڠ‬ฎআȁઐനġ৿ࢬġ ळ޿ඉள‫ڠ‬໦࿤֓‫ڠ‬ฎআȁߪఆġࠉġ;ͼσΑۜஅଷࢄ໦࿤
֓‫ڠ‬ฎআȁ५ୌġࢬ֚ġ ;ͼσΑ྾‫ע‬໦࿤ၑ‫ڠ‬ฎআȁֵாġྶġ‫ဲ܈‬ଷ֒ഥঊࡄ‫ݪ‬໦࿤Ȫ࠳ġਆន;ͼσΑ໦࿤ȫၑ‫ڠ‬ฎআȁਝ५ġഔġอ‫܈‬ଷࢄࡄ‫ݪ‬໦࿤֓‫ڠ‬ฎআȁாനġ‫ܧ‬ġ΀ζȜΐϋΈۜஅછࡄ‫ݪ‬ΓϋΗȜ֓‫ڠ‬ฎআȁષനġਹୀġ ૰ࠐ;ͼσΑ໦࿤֓‫ڠ‬ฎআȁോനġგങġ ֒ഥঊ৖ۛࡄ‫ݪ‬໦࿤֓‫ڠ‬ฎআȁॕ୼ġ୷ଂġ ळ޿ඉள‫ڠ‬໦࿤ၑ‫ڠ‬ฎআȁ଩࿤ġྶဎġ ֒ഥঊໝୋࡄ‫ݪ‬໦࿤֓‫ڠ‬ฎআȁୄ࿤ġࢤġ྾‫ڠاע‬໦࿤֓‫ڠ‬আȁȁୌਕġ݅໌ġ ۜஅ൲໤৘ࡑঔ୭
֓‫ڠ‬ฎআȁಏ࿤ġഔġ֒ഥঊ൲ఠࡄ‫ݪ‬໦࿤ၑ‫ڠ‬ฎআȁ຦୼ġ඾੄ຳġ ֒ഥঊ୆໤‫ڠ‬໦࿤ၑ‫ڠ‬ฎআȁനఆġᐤ֚ġ ;ͼσΑۜஅထཡ‫ݪࡄ້ܙ‬໐࿝֓‫ڠ‬ฎআȁઐനġൽ࣐ġ ૂ༭ഥో໦࿤
֓‫ڠ‬ฎআȁུനġ໌ংġ ळ޿ۜஅ໦࿤
֓‫ڠ‬ฎআȁ౓࢛ġೄ෗ġ ৖ۛൠङ‫ڠ‬Ȫ୆‫ުࢥڠا‬ȫ‫ݪࡄ້ܙ‬໐࿝֓‫ڠ‬ฎআȁ‫݌‬૩ġ༗ġळ༾ଷࢄ໦࿤
֓‫ڠ‬ฎআȁനᰧġგ᫜ġ ۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜၑ‫ڠ‬ฎআȁུࣽġ໲౳ġ ໦ঊ୆໤‫ݪࡄ້ܙڠ‬໐࿝֓‫ڠ‬ฎআȁߧΦ‫ޡ‬ġ੓ġ ۜஅພఠ໦࿤
֓‫ڠ‬ฎআȁఱ୞ġგඃġ ۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜ֓‫ڠ‬ฎআȁܹ‫ؖ‬ġୃങġ ඾ུȆΗͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜĴ࿨૖֥൝ȁDepartment Heads
໹଼24ා4࠮1඾࡛ह

ĵ

ਫ਼ಿ
໗ਫ਼ಿ

‫ޗ‬਎
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֓‫ڠ‬ฎআ
਷֓‫ڠ‬ฎআ

࿒‫ح‬ന ‫ם‬༟ઐ‫ ׆‬஝হ

ۜஅ‫ݪࡄࢹܥ‬໐࿝ȁ໦ঊळ޿‫ڠ‬໦࿤ȁ;ͼσΑۜஅଷࢄ໦࿤
ȁ໦ঊ;ͼσΑ໦࿤ȁ࿪໤ၷ༹໦࿤
ȁۜஅພఠ໦࿤

‫ޗ‬਎
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‫ޗ‬਎

෠‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
਷֓‫ڠ‬ฎআཱ࢛ հ຃‫؂‬ന ోဎઐ‫ ׆‬஝হ੎‫ޗ‬਎֓‫ڠ‬ฎআ५ུ ٗဉ୆ఘཡࢄࡄ‫ݪ‬໐࿝ȁ໦ঊ྾‫ע‬ଷࢄ໦࿤ȁ྾‫ע‬ະ஠৖ۛࡄ‫ݪ‬໦࿤ȁুட྾‫ڠע‬໦࿤ȁळ༾‫ܥ‬ෝ໦࿤ȁ྾‫ڠاע‬໦࿤

‫ޗ‬਎
‫ޗ‬਎
‫ޗ‬਎
‫ޗ‬਎
‫ޗ‬਎

֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ݊౓ ૾࿐‫ ئ‬Ηυ;
ૣၻ ୓౳࿒‫ح‬ന ‫ם‬༟࣏ଳ ં۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝ȁ֒ഥঊ୆໤‫ڠ‬໦࿤ȁ໦ঊ֒ഥࡄ‫ݪ‬໦࿤ȁอ‫܈‬ଷࢄࡄ‫ݪ‬໦࿤ȁૂ༭ഥో໦࿤
ȁळ༾ଷࢄ໦࿤

ȁ
‫ޗ‬਎
‫ޗ‬਎
‫ޗ‬਎
‫ޗ‬਎

ၑ‫ڠ‬ฎআ
ၑ‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
ၑ‫ڠ‬ฎআ࿤ോ ฎ‫ؖ‬ന ٗ૽
ࣞா ૝ࢨ
२࿐ ဉྶ

ඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜȁळ޿ۜஅ໦࿤
ȁ໦ঊࡔಖ‫ڠ‬໦࿤ȁ;ͼσΑ྾‫ע‬໦࿤֒ഥૂ༭৘ࡑΓϋΗȜȁ֒ഥঊ‫ܥ‬ෝٜଢ଼໦࿤
ȁΊΦθૂ༭ٜଢ଼໦࿤
ȁۜஅછιΗΊΦθࡄ‫ݪ‬໦࿤ۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȁࣞພࡔ଻ۜஅછࡄ‫ݪ‬໐࿝ȁȁႉ઄ۜஅછ‫ݪࡄڠ‬ΈσȜίȁȁۜஅळ༾୆໤‫ݪࡄڠ‬ΈσȜίȁȁ;ͼσΑࡄ‫ݪ‬ΈσȜίȁۜஅଷࢄ໐࿝
ȁȁΊΦθພࡔळ޿‫ݪࡄڠ‬ΈσȜίȁȁζρςͺ‫ݪࡄڠ‬ΈσȜίȁȁۜஅછ‫ڠ‬Ȇ྾‫ڠע‬ဏࣣࡄ‫ݪ‬ΈσȜίΓϋΗȜಿȆ‫ޗ‬਎ၑ‫ڠ‬ฎআ

ཱ֔ ੇࢤ

‫ޗ‬਎
‫ޗ‬਎
ΓϋΗȜಿȆ‫ޗ‬਎
‫ޗ‬਎
‫ޗ‬਎
ȁ
ΓϋΗȜಿȆ‫ޗ‬਎ȁၑ‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
ၑ‫ڠ‬ฎআ
࿪‫ڠ‬ฎআ
ၑ‫ڠ‬ฎআཱ֔ ੇࢤ୆ന გၻհ‫ ז‬ચဎ
‫ؖ‬໐ ੳհ‫ ז‬ચဎ

ၑ‫ڠ‬ฎআཱ֔ ੇࢤඅහ‫ޗ‬਎
අහ੎‫ޗ‬਎ȁඅහ‫ޗ‬਎

֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ൥‫ ז‬ဇ‫ج‬ঊ૩ന ‫ם‬঄֓‫ڠ‬ฎআ

๑ന ഓ࿟

੎‫ޗ‬਎

ၑ‫ڠ‬ฎআ‫ࢤ ֔ז‬ਏȁȁȁۜஅ൲໤৘ࡑঔ୭
ۜஅછDNAΙΛί‫ٳ‬อΓϋΗȜ୆ఘ؊൞֒ഥঊٜଢ଼ΓϋΗȜঔ୭ಿȆ‫ޗ‬਎ΓϋΗȜಿȆ‫ޗ‬਎ΓϋΗȜಿȆ‫ޗ‬਎࿪‫ڠ‬ฎআ
ၑ‫ڠ‬ฎআ
࿪‫ڠ‬ฎআ

‫ؖ‬໐ ੳ࿤ോ ฎ‫ؖ‬໐ ੳ

ۜஅછ‫ڠ‬྾‫ڠע‬ဏࣣίυΈρθଔૺ৒ȁࡄ‫ݪ‬ଔૺΈσȜίȁ‫֗ޗ‬ଔૺΈσȜίȁ඾ུȆΗͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜȁȁळ޿ۜஅ໐࿝
ȁȁ;ͼσΑۜஅ໐࿝
ȁ

৒ಿȆਫ਼ಿ
੎‫ޗ‬਎
੎‫ޗ‬਎

֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ
֓‫ڠ‬ฎআ

࿒‫ح‬ന ‫ם‬༟
ఆષ ၻঊ
൥֔ ༠ࣞ

ΓϋΗȜಿȆඅහ‫ޗ‬਎ ֓‫ڠ‬ฎআඅහ‫ޗ‬਎
ভ‫ڠ‬ฎআ
අහ‫ޗ‬਎
֓‫ڠ‬ฎআ

໌ന ೄგ
ຩന ࿆ࢨ
໌ന ೄგ

ఱिȽζΪΡϋۜஅછΓϋΗȜΓϋΗȜಿȆ‫ޗ‬਎අහ੎‫ޗ‬਎਷֓‫ڠ‬ฎআ
਷֓‫ڠ‬ฎআઐ‫ ׆‬஝হ
‫ؖ‬ႅ ۪ਏ

ΟϋΈχ·Ιϋ
Ȫिఱ๷୆໤ພࡄ‫ݪ‬ٛȫ‫ݪࡄ້ܙ‬໐࿝‫ޗ‬਎֓‫ڠ‬ฎআ

઀ୌ‫ם‬ඵ

ಎ؇৘ࡑ৒༶ৣ଻൳պࡓள৘ࡑ৒
ۜஅછ‫ވ‬൳৘ࡑ৒৒ಿȆ‫ޗ‬਎৒ಿȆ‫ޗ‬਎৒ಿȆ‫ޗ‬਎ၑ‫ڠ‬ฎআ
ၑ‫ڠ‬ฎআ
֓‫ڠ‬ฎআ

‫ؖ‬ന ٗ૽
‫ؖ‬ന ٗ૽
‫؂‬ന ోဎ

଎੥৒৒ಿȆ‫ޗ‬਎ၑ‫ڠ‬ฎআ

ཱ֔ ੇࢤ

মྩ໐

মྩಿ

ષദ ࣪ྜ

ࢹ଼֥ȁFaculty & Students૖֥ȁStaff໹଼24ා4࠮1඾࡛ह‫ޗ‬਎

Professor

16‫ݪࡄ້ܙ‬໐࿝‫ޗ‬਎Endowed Chair Professor1අහ‫ޗ‬਎

SA Professor

4

੎‫ޗ‬਎

Associate Professor

14‫ݪࡄ້ܙ‬໐࿝੎‫ޗ‬਎Endowed Chair Associate Professor0අහ੎‫ޗ‬਎SA Associate Professor

4

අහ࣒঍

SA Associate Professor

4

੩‫ޗ‬

Assistant Professor

30‫ݪࡄ້ܙ‬໐࿝੩‫ޗ‬Endowed Chair Assistant Professor1අහ੩‫ޗ‬SA Assistant Professor

10‫ྩޗ‬૖֥

Educational Support Staff

3

ܿ੅૖֥

Technical Staff

3

মྩ૖֥

Administrative Staff

21අහࡄ‫֥ݪ‬SA Researcher47মྩȆܿ੅༞ऎ֥

Part-time General & Technical staff48ȁࠗȁTotal

206

࿨
૖
֥
൝
ȟ
ࢹ
଼
֥

SA ȇ Specially Appointedఱ‫ڠ֭ڠ‬୆ȁGraduate Students໹଼24ා4࠮1඾࡛हฎআ‫ه‬೾

ਘআ‫ه‬೾

Doctor Course

Master Course֓‫شݪࡄࠏڠ‬Graduate School of Medicine

213ၑ‫شݪࡄڠ‬Graduate School of Science311࿪‫شݪࡄڠ‬Graduate School of Pharmaceutical Science01ভ‫شݪࡄڠ‬Graduate School of Dentistry00୆ྵ‫ܥ‬ෝࡄ‫شݪ‬Graduate School of Frontier Biosciences

8

4

ȁࠗ

ȁTotal32

19ɦ๷୆໤ພࡄ‫ݪ‬ਫ਼ͅ෻௺͈৪ͬࠗષ

ࡄ‫֥ݪ‬Ȇࡄ‫ݪ‬୆ȁResearch Fellows & Research Students໹଼24ා4࠮1඾࡛हඅ༆ࡄ‫ڠݪ‬୆Special research students

0

ࡄ‫ݪ‬୆

Research Students

7‫ݪࡄ૽࣭ٸ‬৪

Visiting Research Scholars

1඾ུ‫੅ڠ‬૦‫ޟ‬ٛඅ༆ࡄ‫֥ݪ‬ȪPDȫ

JSPS Research Fellows

3ȁࠗȁTotal11Ķࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅ‫ݪࡄࢹܥ‬໐࿝໦ঊळ޿‫ڠ‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁ෠‫ڠ‬ฎআȁཱ࢛ȁհ຃੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁ૰౓ȁਹਏ੩‫ޗ‬ȁၑ‫ڠ‬ฎআȁհ෼ȁဉਜ਼අහࡄ‫֥ݪ‬ȁΨͼ΂΍ͼ΀ϋΑฎআȁ໛֔ȁၑඅහࡄ‫֥ݪ‬ȁ୆‫ڠشڰ‬ฎআȁȁȁȁȁ࡫ൌȁ͌ͧ࿤ࡄ‫ݪ‬ඤယȇ൚໦࿤͉́Ȃळ޿͈ພࡔ֦ঊ̦਽৽ळ༾‫ܥ‬ෝͅ‫ͬޣג̳͖ݞ‬໦ঊτασٜ́ଢ଼̱̞̀ͥȃ࡛हૺ࣐ಎ͈
ࡄ‫هݪ‬ఴ͉ո‫͈ئ‬೒̜ͤ́ͥȃȪˍȫळ޿଻ΗϋΩ·ඉள͈ࢹ௮͂‫ܥ‬ෝ͈ٜଢ଼ȁ౷‫ݩ‬ષͅంह̳ͥඉ଻໤ৗ͈ಎ́Ȅळ޿଻ΗϋΩ·ඉள͈ඉ଻͉գുഎ̩ࣞͅȂ̷̹͈͘ैဥ̧͉ͩ͛̀ळ༾Ȇດഎ໦ঊඅ։എ̜́ͥȃ൚ࡄ‫ݪ‬৒͉́Ȅຐ඾‫޿ٹ‬ٟঘඉȂΩΑΜτρඉளȂ;΀σΏν޿΀ϋΞυΠ΅ΏϋȄఱಸ޿ळ༾ٟঘ֦ঊ̈́̓ਅș͈ळ޿ඉளͬऺၳͅဥ̞̀Ȃ൲໤Ȅழ૕Ȅळ༾Ȅ໦ঊτασ́ळ޿଻ΗϋΩ·ඉள͈ैဥ
‫ͬࢹܥ‬ٜଢ଼̱̞̀ͥȃ̹͘Ȃ‫ڎ‬ඉள͈‫ܥ‬ෝΡιͼϋ໦ືٜଢ଼̤͍͢ၛఘࢹ௮ٜଢ଼̳̳ͬ͛Ȅၰ৪଼͈‫ͬض‬໵̵̀ඉள͈ࢹ௮͂‫ܥ‬ෝ͈஠ఘ௨͈ၑٜͬ࿒ঐ̱̞̀ͥȃȪˎȫຐ඾‫ٹ‬ພఠ͈ٜଢ଼
ȁຐ඾‫̜ͥ́޿֦ࡔ͈ٹ‬ຐ඾‫͉޿ٹ‬ȂΪΠ͈ષ໐‫ܨ‬ൽۜͅஅ̱̀อै଻‫ٹ‬቞ͬ৽છે̳͂ͥພ‫̳̭ܳͬܨ‬ȃུ޿͉ΪΠ͈͙ͬ਽৽̳̦͂ͥȂ̭͈਽৽අ։଻ͬࠨ೰̳ͥ਽৽௰ါ֦͞ळ޿௰ါ֦͉ະྶ̜́ͥȃ̹͘อै଻‫ٹ‬቞͈อછι΃ΣΒθ͜ྶ̥̯̞̞ͣͦ̀̈́ͅȃ൚ࡄ‫ݪ‬৒͉́Ȃ̭͈ͦͣ͏̹͈̾݃࿚͈ٝ൞ͬං̹ͥ͛Ȃຐ඾‫̷͈͞޿ٹ‬႒‫ͬ޿׻‬ဥ̞̹൲໤ۜஅκΟσ̤̫ͥͅພఠٜͬଢ଼̱̞̀ͥȃ଎ˍȇఉद̈́ळ༾‫ܥ‬ෝͅ‫̳͖ݞͬޣג‬ळ޿ඉளळ޿ඉள͈ఉ̩͉਽৽ळ༾͈ਹါ̈́‫ܥ‬ෝͬਘૐ̳̭ͥ͂̽̀࢘͢ͅၚഎͅैဥͬอ‫̳ͥܞ‬ȃ̳̻̈́ͩळ޿ඉள͈ैဥ‫͈ࢹܥ‬ٜଢ଼͉ळ޿ۜஅພఠ̴͈͙̈́ͣ൲໤ळ༾‫ܥ‬ෝ͈ၑٜ͜ͅ࿨ၛ
̢̞̾͂ͥȃ

ķ଎ˎȇ;΀σΏν޿΀ϋΞυΠ΅Ώϋ͈ၛఘࢹ௮

ࡄ
‫ݪ‬
‫ڰ‬
൲
͈
‫ٽ‬
ါ

଎ˏȇຐ඾‫޿ٹ‬ȪBordetella pertussisȫ႒‫ۯܨ͈׻‬঑෴ࠬછ޿
ȪB. bronchisepticaȫ͂ ႒ ຐ ඾ ‫޿ ٹ‬ȪB. parapertussisȫ͈ Ί Φθ΍ͼΒ͂਽৽අ։଻ȃ5.3 Mbp ͈ΊΦθ΍ͼΒͬ঵̾‫ۯܨ‬঑෴ࠬછ޿͉ఉਅ႒͈൲໤ͬ਽৽ͅ঵̻Ȃ֒ഥঊ͈ഢպࠧ͞
৐́ΊΦθ΍ͼΒ͈઀̯̩̹̈́̽႒ຐ඾‫ۯܨ͞޿ٹ‬঑෴ࠬછ޿͉਽৽ํս͜‫̩ͥ̈́ޛ‬ȃड߃͈యນഎ̈́ა໲
1. Horiguchi, Y. "Swine Atrophic Rhinitis Caused by Pasteurella Multocida Toxin and Bordetella Dermonecrotic
Toxin." Curr Top Microbiol Immunol (2012).
2. Kitadokoro, K., K. Nishimura, S. Kamitani, A. Fukui-Miyazaki, H. Toshima, H. Abe, Y. Kamata, Y. Sugita-Konishi,
S. Yamamoto, H. Karatani, and Y. Horiguchi. "Crystal Structure of Clostridium Perfringens Enterotoxin Displays
Features of {Beta}-Pore-Forming Toxins." J Biol Chem 3;286(22) (2011):19549-55.
3. Kamitani, S., S. Ao, H. Toshima, T. Tachibana, M. Hashimoto, K. Kitadokoro, A. Fukui-Miyazaki, H. Abe, and Y.
Horiguchi. "Enzymatic Actions of Pasteurella Multocida Toxin Detected by Monoclonal Antibodies Recognizing the
Deamidated Alpha Subunit of the Heterotrimeric Gtpase Gq." Febs J 278, no. 15 (2011): 2702-12.
4. Kimura, J. , H. Abe, S. Kamitani, H. Toshima, A. Fukui, M. Miyake, Y. Kamata, Y. Sugita-Konishi, S.
Yamamoto, and Y. Horiguchi. "Clostridium Perfringens Enterotoxin Interacts with Claudins Via Electrostatic
Attraction." J Biol Chem 285, no. 1 (2010): 401-08.
5. Kamitani, S, K. Kitadokoro, M. Miyazawa, H. Toshima, A. Fukui, H. Abe, M. Miyake, and Y. Horiguchi.
"Characterization of the Membrane-Targeting C1 Domain in Pasteurella Multocida Toxin." J Biol Chem 285, no. 33(2010): 25467-75.
ĸࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅ‫ݪࡄࢹܥ‬໐࿝

;ͼσΑۜஅଷࢄ໦࿤
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ‫؂‬നȁోဎ੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁᗣ࿐ȁ੓֚੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁಎ५ȁ‫ם‬๼ȁِș͉΀ͼΒ൝͈;ͼσΑۜஅછ͈ພࡔ଻อ࡛͈໦ঊ‫͈ࢹܥ‬ٜྶͬ࿒ດ̱̞͂̀ͥȃߓఘഎ͉ͅո‫ͬݪࡄ͈ئ‬࣐̞̽̀ͥȃ
ˍȅࢯ HIV ֦ঊ͈ࡄ‫ݪ‬ȁHIV ͉ΙϋΩϋΐȜո‫ͅٸ‬΍σ͞઀൲໤ۜͅஅ଼̦ၛ̱̞̹̈́͛൲໤κΟσ̦ంह̵̴Ȃમळ̈́ພၑ‫ڠ‬എٜଢ଼̢̦࣐̞̈́ȃ΍σ̤̞͉̀ͅȂ;ͼσΑۜஅ͈੝‫ًܢ‬೾Ȫ‫ݙ‬ഢৢȫͬஷ‫֦̳ͥٺ‬ঊ Cyclophilin A ͞ 75,0Į̦ంह̳ͥȃ͉࡛ͩͦͩͦह́͘ͅ 75,0Į͈ࢯ;ͼσΑ࢘‫ ͅض‬C ྎ౤௰͈̞̩̥͈̾ͺηΦॸ̦ਹါ̜̭́ͥ͂Ȃ༷֚́;ͼσΑ͉ 75,0Į͈ດഎ̜́ͥ΃ίΏΡ N ྎ౤௰͈ࡠ̹ͣͦͺηΦॸͬ་։̵̯̀ 75,0Įͥۜ͢ͅஅဲଷ̥
ͣ൪๰̱̤̀ͤȂ;ͼσΑ͂਽৽֦ঊ 75,0Į̦ૺ‫ً͈ا‬೾́ᶕ̨̜̞̭̽̀ͥ͂ͬা̱̀ြ̹ ( ଎ˍ)ȃ̹͘Ȃୌͺές΃͈ HIV-2 ۜஅ৪̤̞͉̀ͅȂ΃ίΏΡ̴͈̥ͩˍͺηΦॸ͈֑̞̦ࠬಎ;ͼσΑၾ͂௖‫̥̭̳ͣ͂ͥ۾‬Ȃ
75,0Į̦୆ఘඤ́৘षͅ;ͼσΑۜஅଷࢄͅ‫ܙ‬ဓ̱̞̭̀ͥ͂ͬྶ̥̱ͣͅȂ;ͼσΑ͈෻Ⴅٜଢ଼ͥ͢ͅထࢃထ
௶ͬ‫خ‬ෝ̱̹ͅȃ̯ͣͅ΍σळ༾ۜͅஅ‫خ‬ෝ̈́་։ HIV-ˍ‫଼଼̱ࢗ͜ͅै͈ڼ‬Ȃ༷֚́΍σΊΦθٜଢ଼ͤۜ͢ͅ
அͅഐ̱̹ࡢఘ͈஖༆ͬ‫خ‬ෝ̱̹͂ȃࣽࢃ̧֨͜௽̧΍σۜஅκΟσࠏ͈‫ږ‬ၛͬ࿒ঐ̳༷֚́Ȃ75,0Į͂;ͼσΑ΃ίΏΡ͈ࢹ௮‫ܥ‬ෝٜଢ଼࣐̞ͬȂ૧̱̞ࢯ HIV ࿪‫ٳ‬อ͈͒؊ဥͬদ͙ͥ .

75,0Į͈ດഎ̜́ͥ;ͼσΑ΃ίΏΡ͈ˏষࡓࢹ௮κΟσȃPȂAȂ̜̞͉ͥ Q ́া̱̹ˍͺηΦॸ͈་‫ͤ͢ͅا‬σȜί͈ࢹ௮̦ఱ̧̩་
‫̳ͥا‬ȃ

75,0Į͈ C ྎ౤ႀ֖ȪSPRY ႀ֖ȫ͈२ষࡓࢹ௮
κΟσȃ;ͼσΑۜஅဲଷͅਹါ̈́ͺηΦॸ͉Ȃ൳̲໹࿂ષͅ໼͐ȃV1 V2 ͉Ȃਅ‫́ۼ‬་։ͅີ͚ႀ֖ȃ଎ˍȇ75,0ĮȪC ྎ౤ȫ͂΃ίΏΡ (N ྎ౤ ) ͈ࢹ௮ĹˎȅΊΦθ‫ݪࡄڠע‬ȁࢯ HIV ࿪ͥ͢ͅ໗ैဥ͈੄࡛͉ͅࡢఘओ̦ంह̳ͥȃۜஅ৪͈ఉ̞Ηͼ࣭͈͂‫ވ‬൳ࡄ‫́ݪ‬Ȃ໗ैဥ͈ઁ̞̈́࿪
໤ൎဓȪΞȜρȜιȜΡ֓ၷȫ͈‫ږ‬ၛͬ࿒ঐ̱̞̀ͥȃ̭ͦ́͘ͅ࿪૮͂ HLA-C ͈͂‫߸۾‬ȂࢯτΠυ;ͼσΑ࿪

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͈ࠬಎෛഽ͂ΙΠ·υȜθ֒ഥঊ͈͂‫̱̥ͣͅྶͬ߸۾‬Ȃ࡛ह͉ডৗయ৫։ુ͞ଅ‫ܥ‬ෝ։ુ͈໗ैဥͅ‫ͥͩ۾‬ఉ߿ͬౝ̱̞॑̀ͥȃˏȅHIV-1 ΊΦθ RNA ඵၾఘ‫̳ͥ۾ͅا‬ٜଢ଼
HIV-1 ͬ‫͚܄‬τΠυ;ͼσΑ͈εΐΞͻήུ֚ङ RNA ΊΦθ͉;ͼσΑၥঊඤ́๱‫ވ‬ခഎࣣ̱ࠫ̀ͅඵၾఘͬ
ࠁ଼̱̞̀ͥȃΊΦθඵၾఘ‫͉ا‬ΊΦθΩΛΉȜΐϋΈȂ‫ݙ‬ഢৢȂΊΦθழ̢۟̈́̓;ͼσΑ୆‫͈۪ڰ‬အș̈́౲‫ٴ‬́ਹါ̈́࿨‫̤̱̹ͤ̀ضͬڬ‬ȂHIV ΊΦθඵၾఘ‫ܥا‬੬͈ٜྶ͉ȂHIV ଷգ͈౤੣̠͂̈́ͤͥȃِș͉ HIV ၥঊ͈ۜஅ଻‫ڕ‬ංͅຈଌ̈́ၥঊ଼੃ً͈೾̷͈ͬ‫ڎ‬౲‫́ٴ‬೪গ̵̯Ȃၥঊඤ HIV-1 ΊΦθඵၾఘ‫ેا‬ఠٜͬଢ଼̱̹ȃ̷͈ࠫ‫ض‬Ȃၥঊ଼੃ૺ࣐͈‫ڎ‬౲‫ྀͅٴ‬ΊΦθඵၾఘ‫࣐̱࣐̀̽̀ૺ̴̱̾ઁ̦੃̷଼͈͂ا‬๭‫ݙ‬ഢৢෝ͈‫ڕ‬ංͅঢ̭̦ͥ͂ྶ̥̹ͣ̈́̽ͅȪ଎ 2Aȫȃ̹͘ȂΊΦθ RNA ષ͈ඵၾఘ‫ا‬ΏΈ΢σȪDLSȫ͈ຈါ਱໦ႀ֖ඤ໐͈‫ܖ؂‬చࠁ଼̞̾̀ͅඊু͈ٜଢ଼ࠏͬߐঀ̱̀મळٜ̈́ଢ଼࣐̞ͬȂಇୟ̱̹ΟȜΗͬࡓࠗͅॳ‫ ͤ͢ͅڠشܥ‬DLS ႀ֖͈
RNA ၛఘࢹ௮͈κΟςϋΈ࣐̹ͬ̽ࠫ‫ض‬Ȃ͉ࣽ́͂͘஠̩։̈́ͥȂΏνȜΡΦΛΠအࢹ௮ͬ‫̺ͭ܄‬૧̱̞ࢹ௮κΟσͬං̹ȃ̭͈ΏνȜΡΦΛΠအࢹ௮͉ DLS ஠ఘ͈ࢹ௮ͬ‫̩ܰޑ‬೰̱ȂΑΞθ͈α·Πσͬࢰ௵̳ͥ࿨‫ضͬڬ‬̹̱̞̞̀ͥͩ͊ DLS ͈ါ̜́ͤȂ̭ͦͬດഎ̱̹͂૧ܰࢯ;ͼσΑၷ༹͉ఱ̧̈́‫خ‬ෝ଻ͬ঵̞̽̀ͥ͂ࣉ̢ͣ
̹ͦȪ଎ 2Bȫȃ଎ˎȇHIV ΊΦθඵၾఘ‫ا‬ड߃͈యນഎ̈́ა໲
1. A proposal for a new HIV-1 DLS structural model. Sakuragi JI, Ode H, Sakuragi S, Shioda T, Sato H. Nucleic Acids
Res. In press.
2. Role of human TRIM5Į in intrinsic immunity. Nakayama EE, Shioda T. Front Microbiol.2012;3:97.
3. TRIM5Į and Species Tropism of HIV/SIV. Nakayama EE, Shioda T. Front Microbiol. 2012; 3:13.
4. The relationship between HIV-1 genome RNA dimerization, virion maturation and infectivity. Ohishi M, Nakano T,
Sakuragi S, Shioda T, Sano K, Sakuragi JI. Nucleic Acids Res. 2011 Apr;39(8):3404-17.
5. HLA-Cw*04 allele associated with nevirapine-indued rash in HIV-infected Thai patients. Likanonsakul S,
Rattanatham T, Feangvad S, Uttayamakul S, Prosithsirikul W, Tunthanathip P, Nakayama EE, Shioda T. AIDS Res
Ther. 2009 Oct 21;6:22.
ĺࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅ‫ݪࡄࢹܥ‬໐࿝໦ঊ;ͼσΑࡄ‫ݪ‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁȁȁ਷֓‫ڠ‬ฎআȁઐ‫׆‬ȁ஝হ੩‫ޗ‬ȁȁȁ֓‫ڠ‬ฎআȁȁ‫ུؖ‬ȁഔ੩‫ޗ‬ȁȁȁ֓‫ڠ‬ฎআȁȁ໛ࡔȁଢٚඅහ੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁȁ‫ࡔێ‬ȁဉഴඅහࡄ‫֥ݪ‬ȁ෠‫ڠ‬ฎআȁȁ઀࿤ȁ૥ঊඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁȁུఆȁܲ঎εΑΡ·ȁȁ਷֓‫ڠ‬ฎআȁ‫ح‬൥ȁఱ঎ࡄ‫ݪ‬ඤယȇ൚ࡄ‫ݪ‬৒͉́Ȃ˟߿۴‫ͼ;׶‬σΑ (HCV) ͈ۜ਋଻ळ༾͈͒૟වȂໝୋȂ̷̱̀อછພၑ͈໦ঊ‫ܥ‬੬͈ٜଢ଼͂Ȃ֒ഥঊহၷͅຈଌ̈́૧̱̞֒ഥঊ൵වα·ΗȜ͈‫ٳ‬อͬૺ̞͛̀ͥȃˍȅHCV ͈໦ঊ୆໤‫ڠ‬ȁଲ‫͈ٮ‬௙૽࢛͈ 3% ̦‫ ͅܡ‬HCV ۜͅஅ̱̀
̤ͤȂ̷͈࿩ 8 ‫଻ྕ̦ڬ‬঵௽ۜஅ͒͂֊࣐̱Ȃఉ̩̦۴ࣁ་ͬࠐ̀۴ळ༾‫ͬ܈‬อછ̳ͥȃِ̦࣭͉́ࣞۜഽ̈́ࡃ͈ࠬΑ·ςȜΣϋΈ༹̦൵ව̯ͦȂ࿶ࠬͥ͢ͅ HCV ۜஅ͉͕͖ଷգ
̯̹̦ͦȂ‫ ͅܡ‬200 ྔ૽͈ۜ͜அ৪̦ంह̳
ͥȃͼϋΗȜέͿυϋ (IFN) ͬಎ૤̱̹͂ࢯ
;ͼσΑহၷ࣐̠ͬ͜ 30% ೾ഽ͈ۛ৪͉́;ͼσΑͬક৐̵̧̯̭͉̞ͥ͂́̈́ȃ̷͈হၷ࢘‫ ͂ض‬IL28B ͈֒ഥঊఉ߿͞ IP-10 ͈อ࡛ၾ̦௖‫̦̭̳͂ͥ۾‬౶̞̦ͣͦ̀ͥȂ̷͈‫ܥ‬ࢹ͉ྶ̥̞ͣ́̈́ȃȁHCV ၥঊ͉ळ༾ນ࿂͈ၤॸఉൠ႒ͅ༛௴̯
ͦȂ΀ϋαυȜί౩ฒৗ̱ͬٚ̀਋ယఘ࢓༞໦ঊࣣ̱͂ࠫȂ΀ϋΡ΍ͼΠȜΏᾼ̽̀͢΀ϋΡΖȜθඤࣺ৾ͤͅ
ͦͥ͘ȃHCV ͉ఈ͈έρΫ;ͼσΑ‫ͼ;͈ش‬σΑ͂൳အͅȂ;ͼσΑΊΦθ̦ mRNA ̱͂̀൱̩ίρΑङ RNA;ͼσΆȂΊΦθ RNA ̥ͣ࿩ 3000 ͺηΦॸ͈ஜߐఘ౩ฒৗཱུ̦࿫̯ͦͥȃ;ͼσΑΊΦθ͈ 5’ ๱ཱུ࿫ႀ֖ͅ
͉ IRES(Internal Ribosomal Entry Site) ̦ంह̱Ȃ΅λΛί๱ջంഎཱུ̈́࿫ͬংͥȃ۴௫අ։എ̈́ microRNA ̜́ͥ
miR-122 ̦ 5’ ๱ཱུ࿫ႀ֖ͅೄ୪ࣣ̱ࠫȂ;ͼσΑΊΦθཱུ͈࿫ͬᄤૺ̱̳̭̦ͥ͂ȂHCV ۜஅ͈۴௫අ։଻ͅ‫۾‬ဓ̱̞̀ͥȃ
ȁHCV ࡄ‫͉ݪ‬৘ࡑ৒‫ ̜ͥ́ڼ‬JFH1 ‫( ڼ‬HCVcc) ͈ࢹಃͤ͢ͅ๲࿬എͅૺ̺̦ͭȂພࡔ଻ͬ༗঵̱̹࿤୆‫͈ڼ‬෽ူࠏ͉ྚ̺‫ٳ‬อ̧̞̞́̀̈́ȃड߃ȂmiR-122 ͬ‫ޑ‬ଷอ̵࡛̯̭ͥ͂̽̀͢ͅȂအș̈́ळ༾‫ ́ڼ‬HCVcc ͬ௩໙̧́
̭̦ͥ͂ྶ̥̹ͣ̈́̽ͅȃ૧̹ͅ‫ٳ‬อ̯̹ͦۜ਋଻ळ༾‫ͬڼ‬ဥ̞̀Ȃۛ৪ࠬୄဇြ͈ HCV ͈௩໙ͬদ͙̞̀ͥȃȁ࡛हȂ;ͼσΑ͈εςιρȜΔ͞ίυΞͺȜΔͅచ̳ͥஷ‫ٺ‬स͈‫ٳ‬อ̦ૺ࣐̱̞̦̀ͥȂ࿪सఛ଻;ͼσΑ͈੄࡛̦ఱ̧̈́࿚ఴ̞͂̈́̽̀ͥȃ;ͼσΑ౩ฒৗͬດഎ̳ͥͅ࿪सͅ๤͓̀Ȃໝୋͅຈଌ̈́਽৽֦ঊͬດഎ̱̹͂࿪स͈༷̦Ȃఛ଻;ͼσΑ͈੄࡛ၚ̦೩̞ത́ခ၌̜́ͥȃ̷̭́Ȃ
HCV ͈ໝୋ͞਽৽྾‫۬ע‬ণ‫͈̥ͣࢹܥ‬ٝ๰ͅ‫۾‬ဓ̳ͥ਽৽֦ঊͬ൳೰̱ȂC ߿ྕ଻۴‫͈׶‬૧̱̞஻࿪ΗȜΊΛΠͬ࠿̱̞॑̀ͥȃ̹͘ȂHCV ۜஅͥ͢ͅডཛ۴͞۴ळ༾‫͈܈‬อછ‫͈ࢹܥ‬ٜଢ଼ͬȂHCV౩ฒৗͬอ࡛̳ͥΠρϋΑΐͿΣΛ·ζ;Αͬဥ̞̀ૺ̞͛̀ͥȃĲıˎȅΨ΅νυ;ͼσΑα·ΗȜ͈‫ٳ‬อ
HCV ͈̠͢ͅ෽ူळ༾́ໝୋ̧̞́̈́;ͼσΑۜஅછ͈ࡄ‫͉ͅݪ‬Ȃ;ͼσΑα·ΗȜ̦ਹါ̈́໌‫ͥ̈́͂ܕ‬ȃ̹͘Ȃ୶౤֓ၷ͈ါ͂̈́ͥ֒ഥঊহၷ͉ͅȂհ஠́֒ഥঊ൵ව࢘ၚ̦̞ࣞȂ֒ഥঊ൵වα·ΗȜ͈‫ٳ‬อ̦ະ‫̜ͥ́ࠧخ‬ȃ

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ِș͉अಖ;ͼσΆ̜ͥΨ΅νυ;ͼσΑ Autographa californica nucleopolyhedrovirus (AcNPV) ͬ၌ဥ̱̹ఉ
‫ܥ‬ෝ;ͼσΑα·ΗȜ‫ٳ‬อͬૺ̞͛̀ͥȃAcNPV ͉ 134kbp ͈۪ે 2 ུङ DNA ͬΊΦθ̱͂̀঵̻Ȃۜஅळ༾͈ 30 ȡ 40ɓ̦ఉ‫ڙ‬ఘ౩ฒৗͅ౾̧͕͈۟ͩͥ̓‫ޑ‬ႁ̈́ఉ‫ڙ‬ఘίυκȜΗȜͬခ̱̞̀ͥȃ̭͈଻ৗͬ၌ဥ̱̀Ȃ
AcNPV ͉अಖळ༾ͬဥ̞̹ழ̢۟౩ฒৗ͈ॲ୆ࠏ̱͂̀၌ဥ̯̞ͦ̀ͥȃ̹͘ȂAcNPV ͉अಖळ༾̴͈͙̈́ͣȂࢩํ̈́ሶ඿൲໤ळ༾ͅȂ࢘ၚ̩͢‫ٸ‬ြ֒ഥঊͬ൵ව̧̭̦́ͥ͂฻ྶ̱Ȃ૧̱̞֒ഥঊ൵වα·ΗȜ̱͂̀‫ͬ࢕ݗ‬ဵ͍ͥম̹͂̈́̽ȃAcNPV ͉ఱ̧̈́‫ٸ‬ြ֒ഥঊͬழ͙ࣺ̺ͭழ̢۟;ͼσΑͬ۰ౙͅैୋ̧́Ȃ̱̥͜Ȃሶ඿൲໤ळ༾͉́஠̩ໝୋ̱̞̭̥̈́͂ͣȂͺΟΦ;ͼσΑα·ΗȜ൝́࿚ఴ͂̈́ͥুၛ௩૓;ͼσΑ͈੄࡛͞Ȃ;ͼσΑ౩ฒৗ͈อ࡛ͥ͢ͅခ‫̈́ٺ‬྾‫ע‬؊൞͈ည൵൝͈‫ܓ‬ᏼ̦̞̈́ಿਫ਼̦̜ͥȃ̹͘ȂAcNPV ͬ๽࣌ඤൎဓ̯̹ͦζ;Α̦Ȃ౿ঘၾ͈ͼϋέσ΀ϋΎ;ͼσΑ͈ࢲ̥ࠢͣۖ஠ͅཡࢄ̯̭̦ͦͥ͂༭̯࣬ͦȂळ༾ඤࣺ̹৾ͤͦ͘ͅ
AcNPV ΊΦθ̦ȂToll-like receptor 9(TLR9) ̱ͬٚ̀྾‫֒ע‬ഥঊ͈อ࡛ͬည൵̱̞̭̀ͥ͂ͬྶ̥̱̹ͣͅȃ଼̭͈ୡ͉ȂΨ΅νυ;ͼσΑ̦֒ഥঊ൵වα·ΗȜ̱̺̫̩͂̀́̈́Ȃ୪ਅࠐႹ͉̽̀͢ͅͺΐνΨϋΠ‫ͬ଻ڰ‬໵̵঵̹̽૧̱̞χ·Ιϋα·ΗȜ̱͈͂̀‫خ‬ෝ଻ͬাऐ̳͈̜ͥ́ͥ͜ȃड߃͈యນഎ̈́ა໲
1. Abe T, Fukuhara T, Wen X, Ninomiya A, Moriishi K, Maehara Y, Takeuchi O, Kawai T, Akira S, Matsuura Y.
CD44 participates in the IP-10 induction in cells replicating HCV RNA through an interaction with TLR2 andhyaluronan. J Virol. 2012.
2. Kataoka C, Kaname Y, Taguwa S, Abe T, Fukuhara T, Tani H, Moriishi K, Matsuura Y. Baculovirus
GP64-mediated entry into mammalian cells. J Virol. 2012 Mar;86(5):2610-20.
3. Kambara H, Fukuhara T, Shiokawa M, Ono C, Ohara Y, Kamitani W, Matsuura Y. Establishment of a novel
permissive cell line for the propagation of hepatitis C virus by expression of microRNA miR122. J Virol. 2012
Feb;86(3):1382-93.
4. Taguwa S, Kambara H, Fujita N, Noda T, Yoshimori T, Koike K, Moriishi K, Matsuura Y. Dysfunction of
autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 2011 Dec;85(24):13185-94.
5. Katoh H, Mori Y, Kambara H, Abe T, Fukuhara T, Morita E, Moriishi K, Matsuura Y. Heterogeneous nuclear
ribonucleoprotein A2 participates in the replication of Japanese encephalitis through an interaction with viralproteins and RNA. J Virol. 2011 Nov;85(21):10976-88.
ĲĲࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅ‫ݪࡄࢹܥ‬໐࿝ۜஅພఠ໦࿤
ࡄ‫ݪ‬ΈσȜί੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ५ུȁٗဉ੩‫ޗ‬ȁȁၑ‫ڠ‬ฎআȁॕ֔ȁ๼გȁ൚ࡄ‫ݪ‬৒͉́Ȃ༾ঊಖ႒ພࡔ଻‫ܙ‬୆ಖ̜́ͥΠ΅ΕίρΒζͬκΟσ̱͂̀‫ܙ‬୆ಖ͈਽৽྾‫ဲע‬ଷ‫ͅࢹܥ‬಍࿒̱̹‫ܙ‬୆ಖ྾‫ݪࡄڠע‬ȁ৾ͤͅழ̞ͭ́ͥȃˍȫΠ΅ΕίρΒζ͉͂‫̥ة‬ȉȁΠ΅ΕίρΒζછ͉Π΅ΕίρΒζࡔಖȪToxoplasma gondiiȫ͈ۜஅ̧̭̯ͤ֨ܳͦͥ͢ͅࡔಖພ̜́ͥȃ̷͈୆‫͉۪ڰ‬Υ΋ͬਞ਽৽̱͂̀ΪΠȆΥΒη̈́̓ͬ‫͚܄‬஠͈̀ࢭ‫أ‬Ȫ‫ࠬأ‬ȫ൲໤̦ಎ‫ۼ‬਽৽̭̥͂̈́ͥ͂ͣ๱ુͅࢩ
̞਽৽֖ͬ঵̻Ȃۜஅ̱̹Υ΋͈໯༒̦ంह̱̠۪ͥ‫΄͈́ޏ‬ȜΟΣϋΈۜ͞அ̱̞̀ͥ‫ز‬಄͈୆රͬ‫ح‬෎ੜၑ̵̴୫̳̭৾ͥ͂ͥ͢ͅȂ̴̞ͦ͜ࠐ࢛ۜஅ̽̀͢ͅΪΠۜͅஅ̳ͥȃΠ΅ΕίρΒζࡔಖ͉࠲ુ૽͉͕͈́͂ͭ̓
ાࣣۜஅ੝‫ۜͅܢ‬ཙအછે͞ςϋΩ୯͈ࠚ̞ਆಷ೾ഽ͈છેͬা̱ະࡐ଻ۜஅ͂̈́ͥ඾გࡉພࡔఘ̜́ͤȂ஠ଲ‫ٮ‬എ͉ͅଲ‫ ͈࢛૽ٮ‬3 ໦͈ 1 ̦ະࡐ଻ۜஅ̱̞̯̀ͥ͂ͦͥȃ਽৽྾‫ैͅુୃ̦ࠏע‬൲̱̞̀ͥાࣣ͉́஠̩࿚ఴ͂
͉̞̦̈́ͣ̈́Ȃ߃ා͈΀ͼΒȪࢃഛ଻ະ஠྾‫ע‬છ࢓߲ȫͬอછ̱̹ۛ৪̤̞̈́̓̀ͅ΃ςΣ෹‫́ͭͣ̈́͂׶‬Π΅ΕίρΒζෞછ̦ঘ֦͈৽ါ͈̈́ͬ͜୸͛ͥȃ̹͘ළૡশ༦ఘ͉։໤̜́ͥథ঱ͬ‫̹̞̱֚͛̈́ͅ୲ݵ‬ਅ͈྾‫ע‬ະ஠ેఠ̞̦͂̈́̽̀ͥȂ̷͈षͅළິ̦੝ۜஅ̜̹́̽ાࣣథ঱ͅΠ΅ΕίρΒζࡔಖ̦ۜஅ̱ၠॲȂ̜̞͉ͥۜஅ
̱̹͘͘૧୆঱̦କ൮છȆ઀ෞછͬ୶ഛഎͅ༴̢̹ેఠ́౪୆̷̱͈ࢃ͈ୈ૰อ֗Ȃಶ‫ܥژ‬ෝ೩‫୶ฺ̠ͬئ‬ഛ଻৖̧̭̳ۛͬ֨ܳΪΠਹါພࡔఘ̜́ͥ 2)ȃ̭͈̠͢ͅȂ;ͼσΑۜஅછȆळ޿ۜஅછȆ૯޿ۜஅછͅ‫ࡔ̢̀ح‬ಖȪ‫ܙ‬୆ಖȫۜஅછِ͜ș͈૸߃ͅంह̳ͥۜஅછ̜́ͥȪ଎ˍȫȃˎȫΠ΅ΕίρΒζ͉਽৽͈྾‫ဲͬࠏע‬ଷ̳ͥȁΠ΅ΕίρΒζࡔಖ͉֒ഥঊ߿͈֑̞̥ͣȂ৽̱͂̀ I ߿ȂII ߿̷̱̀ III ߿ࡔಖ̞̠͂ 3 ͈̾֒ഥঊ߿ͅ໦̫
ͣͦͥȃȶ୆̧̹ȷΠ΅ΕίρΒζࡔಖͬۜஅ̵̯ͥ͂ζ·υέ͹Ȝΐ͞ਏેळ༾̤̞̀ͅ‫׶‬છ଻΍ͼΠ΃ͼϋ͈ॲ୆͈̈́̓ুட྾‫ע‬؊൞̦ৼ̯̦ܳͦͥȂ̭͈ȶ୆̧̹ȷࡔಖͥ͢ͅুட྾‫ע‬؊൞ͅ‫ڼ‬අ։଻̦ంह̳̭̦ͥ͂া
ऐ̯̹ͦȃ̷͈ඤȂII ߿ࡔಖ͉́ I ߿͂ III ߿ࡔಖ͂։̈́ͤ਽৽ুட྾‫ע‬؊൞ͬဲଷ̧̞̭̦́̈́͂ոஜ̥ͣ౶ͣ
̞̹̦ͦ̀Ȃِș͉Π΅ΕίρΒζ̦ۜஅশͅດഎळ༾ඤͅ༶੄̳ͥ΀έͿ·ΗȜ໦ঊ͈̜֚̾́ͥ ROP16 ̞̠͂໦ঊ̦਽৽͈ࢯ‫׶‬છ଻͈ඤह଻ίυΈρθͬ‫̭̳́͂ͥا଻ڰ‬྾‫ע‬؊൞ͬဲଷ̳̭ͥ͂ͬা̱Ȃ̯ͣͅ ROP16͈֚ͺηΦॸ͈་։ͤ͢ͅ II ߿ࡔಖ̷͉͈́‫̦଻ڰ‬ಠ̱̩೩̞̭͂ͬࡉ੄̱̹ȁȪ଎ˎȫȃ̯ͣͅ III ߿ࡔಖ͉ I ߿͂ II ߿ࡔಖ͂๤͓̀ζ;Ὰ̤̞͉ͅಠ̱̩೩̞ພࡔ଻ͬခ̳̭̦ͥ͂༭̯̞̹̦࣬ͦ̀Ȃ̷͈໦ঊഎ‫ܥ‬੬ِ̞̾̀ͅș͉ ROP16 ͈έ͹ηςȜ໦ঊ͈̜֚̾́ͥ ROP18 ̦਽৽͈྾‫ע‬؊൞ͅ‫۾‬ဓ̳ͥഢৢ֦ঊ̜́ͥ $7)ȕͬດഎ̷̱͈͂̀໦ٜͬည൵̳̭ͥ͂́਽৽྾‫ע‬؊൞ͬဲଷ̳̭ͥ͂ͬࡉ੄̱̞̀ͥ ( ଎ˏ)ȃȁΠ΅ΕίρΒζ̦༶੄̳ͥ΀έͿ·ΗȜ໦ঊ͉ ROP16 ȟ ROP18 ͈ఈͅତ਱ਅ႒ంह̳̭̦ͥ͂ΊΦθχͼΡٜ̈́ଢ଼̥ͣাऐ̯̞̦ͦ̀ͥȂ̷͈਽৽ळ༾ඤಎ͈́΀έͿ·ΗȜ‫ܥ‬ෝ͉ͅະྶ̈́ത̦ఉ̩Ȃ̷͈ດഎ໦ঊ͉̽͂͜
ະྶ̜́ͥȃਲِ̽̀ș̷͉͈͈֚֚̾̾΀έͿ·ΗȜ࢓༞໦ঊ͈֒ഥঊࠧఅࡔಖͬै଼̱Ȃζ;ᾼ̤̫ͥພࡔ
଻͈ບ‫΀ͅࡓͬث‬έͿ·ΗȜ໦ঊ͈ࣆ̷ࣺ͙͈͂΀έͿ·ΗȜ‫͈ࢹܥ‬ٜྶ̥ͣ૧̱̞਽৽྾‫ͬࠏע‬ౝ̱̠̱॑͂͢
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଎ˍȁΠ΅ΕίρΒζ͉͂ȉȪAȫΠ΅ΕίρΒζࡔಖ͈࿅৆଎ȃ୶౤໐ͅ໦๥઀‫̜ͥ́ۗܕ‬υίΠςȜȪ୤̞໐໦ȫ̦ంह̳ͥȃȪBȫ਽৽ळ༾ͅ
ۜஅ̱̹Π΅ΕίρΒζࡔಖ͈ഩঊࡐ๷‫ޢ‬଎ȃۜஅळ༾ಎ͉́‫ܙ‬୆༾ඤȪକ૗࿦֣ȫͅంह̳ͥȃ̹͘Ȃ୶౤໐ͅྶ
̥ͣ̈́υίΠςȜ௨̦෇͛ͣͦͥȪႂ࿦֣ȫȃȪ&ȫۜஅळ༾ಎ͈́Π΅ΕίρΒζࡔಖ͈྾‫ע‬அ૗଎ȃΠ΅ΕίρΒζࡔಖ͈ळ༾྄౩ฒৗ *$3 ͬ୤૗́ȂROP18 ͬႂ૗́அ૗̱̹ȃંȂROP16 ͜൳အͅυίΠςȜͅంह̳ͥ΀έͿ·ΗȜ໦ঊ̜́ͥȃ଎ 2ȁROP16 ͈ಎ͈֚ͺηΦॸ་։̦྾‫ဲע‬ଷෝͬࠨ೰̱̞̀ͥȃ଎ˏȁ$7)ȕࠧఅζ;Α͉ ROP18 ࠧఅΠ΅ΕίρΒζࡔಖͅచȁȁȁ̳ͥࣞۜ਋଻͂̈́ͥ
‫ܖ‬ৗջంഎอ࢕౩ฒৗσΏέͿρȜΔͬอ࡛̳ͥ ROP18 ࠧఅࡔಖͬȂ࿤୆߿ζ;Α‫ ͍ݞ‬$7)ȕࠧఅζ;ᾼۜஅ̵̯̹ࢃ 8 ඾ࢃͅ୆ఘͼιȜΐϋΈ௡౾ͬঀ̽̀σΏέͿρȜΔ‫௶ͬ଻ڰ‬೰̱̹ȃ$7)ȕࠧఅζ;Ά͉σΏέͿρȜΔอ࢕‫ޑ‬ഽ̦‫̭̞ޑ‬̥͂ͣȂROP18 ࠧఅࡔಖ̦ࢩ̩‫ڐ‬८̱̞̭̦̀ͥ͂াऐ̯ͦͥȃड߃͈యນഎ̈́ა໲
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ĲĴࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities୆ఘཡࢄࡄ‫ݪ‬໐࿝໦ঊ྾‫ע‬ଷࢄ໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁȁ֓‫ڠ‬ฎআȁ݊౓ȁ૾੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁհ‫ݳ‬ȁܵ૽੩‫ޗ‬ȁȁ֓‫ڠ‬ฎআȁुࡔȁਘ໹අහࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁ૩നȁ࠲ఊ჊අහࡄ‫֥ݪ‬ȁ࿪‫ڠ‬ฎআȁධ౓ȁ໌੉අහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁŐŭŪŷŪŢġłįġŔŪŮŮŢ
අහࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁńũŢŪŶĮŚŶŢůŨġŕŴŢŪ

ˍȅςϋΩ‫ͬݩ‬̱̹ٚ྾‫ע‬؊൞଼ၛ͈໦ঊ‫ࢹܥ‬ȁT ळ༾͉ࢯࡔ೹াळ༾ષ͈ MHC ໦ঊ̽̀͢ͅ೹া̯̹ͦࢯࡔ໦ঊͬ෇ে̱ȂΰσΩȜ T ळ༾͞΀έͿ·ΗȜ T ळ༾͒͂໦‫̳ͥا‬ȃ༷֚Ȃࢯࡔঁࠣ͂ΰσΩȜ T ळ༾͈༞੩͈‫ئ‬ȂB ळ༾͉ࢯఘॲ୆ळ༾ܱ͞‫ ؛‬B ळ༾ͅ໦‫̳ͥا‬ȃ̭͈̠̈́͢ςϋΩ‫͈ݩ‬໦‫ًا‬೾͉ͅ T ळ༾Ȇࢯࡔ೹াळ༾‫ ͍ݞۼ‬T ळ༾ȆB ळ༾‫͈ۼ‬໤ၑഎ̈́௖ࡽैဥ̦ຈଌ̜́ͤȂ̭ͦͣ௖ࡽैဥ͉ CD40ȂCD40 ς΄ϋΡȂB7ȂCD28 ൝͈༞੩ঁࠣ໦ঊ̽̀͢ͅ౜̞ͩͦ̀ͥȃड߃ȂΓζέ΁ςϋέ͹
ηςȜͅ௺̳ͥ໦ঊ̦Ȃਅș͈྾‫ע‬؊൞̤̞̀ͅਹါ̈́࿨‫̧̹̥̀̽̈́ͣͅྶ̦̭̞̱̹͂ͥ̀ضͬڬ‬ȃ̹͘Ȃ
CD40 ͈ΏΈ΢σഥో໦ঊ TRAF3 ̷̤͍͈͢‫۾‬Ⴒ໦ঊ̦ȂB ळ༾୆ం͞໦‫ͅا‬ਹါ̜̭́ͥ͂͜ྶ̥̞ͣ͂̈́̽̀ͥȃ൚ࡄ‫ݪ‬໦࿤͉́Ȃ̭ͦͣςϋΩ‫ໍͬݩ‬ర̱͂̀߫ͤࢩ̬ͣͦͥအș̈́྾‫ע‬ଷࢄ໦ঊͥ͢ͅ྾‫ע‬฽؊಺୯‫ͬࢹܥ‬ٜଢ଼̱̞̀ͥȃˍȫ྾‫ע‬Γζέ΁ςϋͥ͢ͅ྾‫ע‬ଷࢄ‫ࢹܥ‬ȇȁΓζέ΁ςϋέ͹ηςȜ໦ঊ͉૰ࠐ͈৊ॐͅచ̱̀‫ڠا‬฽อ‫ڠا͞଻ڰ‬ည֦‫ͬ଻ڰ‬อ‫̱ܞ‬Ȃ̷͈૝ಿ༷࢜ͬࠨ೰̳
ͥ૰ࠐ৊॑΄ͼΘϋΑ֦ঊ̱͂̀౶̞̦ͣͦ̀ͥȂ൚໦࿤͈ࡄ‫̥ͣݪ‬Ȃତਅ႒͈Γζέ΁ςϋ໦ঊ̦྾‫ע‬฽؊͈အș̈́ΑΞΛί́‫ܥ‬ෝ̱̞̭̦̀ͥ͂ྶ̥̤ͣ̈́̽̀ͤͅȂ૧̹̈́྾‫ע‬ଷࢄ໦ঊέ͹ηςȜȪ྾‫ע‬Γζέ΁ςϋȫ̦ࠁ଼̯̜ͦ̾̾ͥȪ଎ˍȫȃ႕̢͊ȂSema4D ͉ B ळ༾͈‫֋଻ુࢭ͞ا଻ڰ‬঵ͅ‫ܙ‬ဓ̳ͥ͂͂͜ͅȂਏેळ༾͈‫ͬا଻ڰ‬̱ٚ̀ळ༾଻྾‫಺͈ע‬୯͜ͅ‫۾‬ဓ̱̞̀ͥȃ༷֚ȂSema4A ͉ೄ୪ T ळ༾ͅ൱̞̀ȂT ळ༾͈ίρͼηϋΈ͞ Th1 ໦‫ͅا‬ਹါ̈́࿨‫̳̹ضͬڬ‬ȃ̹͘ȂSema6D ̷͈͂਋ယఘ Plexin-A1 ͈௖ࡽैဥ͉Ȃਏેळ༾͈‫ا଻ڰ‬Ȃ෫ࣸळ༾͈໦‫ا‬ည൵ͅຈါ̜́ͥƢࢵͅȂ‫ ا଻ڰ‬T ळ༾ષ͈ Sema7A ̦ĮLQWHJULQ ̱ͬٚ̀ζ·υέ͹Ȝΐͬঁ̱ࠣ̀Ȃ‫׶‬છ฽؊͈֨
̧߄̩̭ͬ֨͂͜ྶ̥̞ͣ̈́̽̀ͥͅȪ଎ˎȫȃ଎ˍȅయນഎ̈́྾‫ע‬Γζέ΁ςϋ

଎ˎȅT ळ༾Ȟζ·υέ͹Ȝΐ‫ۼ‬ೄ୪௖ࡽैဥ
̤̫ͥͅ Sema7A ͂ĮLQWHJULQ ͈྾‫ע‬Ώ΢ίΑ͈͒ާਬˎȫB ळ༾໦‫ا‬Ȇ୆ంଷࢄ͈໦ঊ‫ࢹܥ‬ȇȁB ळ༾͉‫ٸ‬ြࢯࡔͅచ̱̀࢘‫ض‬എ̈́ࢯఘͬॲ୆̳̹ͥ͛ͅࢯఘॲ୆ळ༾ܱ͞‫ ؛‬B ळ༾ͅ໦‫̦̳ͥا‬Ȃ̷͈षͅ B ळ
BAFF-R ͅయນ̯ͦͥ TNF τΓίΗȜέ͹ηςȜ̥͈ͣঁࠣͬຈါ̳͂ͥȃ༾ນ࿂ષ͈ B ळ༾ࢯࡔτΓίΗȜ͞ CD40Ȃ̭͈ͦͣΏΈ΢σഥోࠐႹͅ‫۾‬ဓ̳ͥ໦ঊ‫ܥ‬ෝ̦൚໦࿤͈ࡄ‫̥ͦ̾ͥ̈́ͣͅͅྶͤ͢ͅݪ‬Ȃ‫଻ס‬྾‫଼ע‬ၛ‫̷͈͂ࢹܥ‬ୖ৻଻ͥ͢ͅ྾‫ע‬ພఠ͈อછ‫̦ࢹܥ‬ٜྶ̧̯̹ͦ̀ȃඅͅȂCD40ȂBAFFR ळ༾ඤႀ֖ࣣ̳ٛͥͅ TRAF3 ໦ঊ̦ȂBळ༾͈୆ంͅຈଌ̜̭̦́ͥ͂ྶ̥ͣ͂̈́ͥ͂͂͜ͅȂTRAF3 ࣣٛ໦ঊ̜́ͤḀ̑̾ B ळ༾ࢯࡔτΓίΗȜΏΈ΢σ͈‫ئ‬ၠͅంह̳ͥ PKC έ͹ηςȜ໦ঊȂPKNˍ̦ $NW ͈Υ΄Ξͻήଷࢄ໦ঊ̱͂̀ैဥ̱Ȃুࡨ฽؊଻ B ळ༾ੰ‫ݲ‬൝͈྾‫ۘע‬ယ଼ၛͅຈါ̜̭́ͥ͂͜ड߃ࡉ੄̧̯̹ͦ̀ȃˎȅ਽৽ - ພࡔఘ‫ۼ‬௖ࡽैဥͥ͢ͅ྾‫ע‬ພఠอછ͈໦ঊ‫ࢹܥ‬ȁ(SVWHLQ%DUU ;ͼσΑȪEBVȫ͉஠ଲ‫ͅํࢩ́ٮ‬ஆ໖ۜஅ̱̞̀ͥ B ळ༾ঐ࢜଻ΪΠ̦ͭΰσβΑ;ͼσΆ̜ͥȃ୆ఘඤ͉́ુͅ;ͼσΑໝୋ̷͈͂ෳੰͬ߫ͤ༐̱̀ۜஅ໹࣑ͬ༗̞̦̽̀ͥȂ‫ح‬ႢȂ௫‫֊ܕ‬૒ฺ̠ͅ྾‫ဲע‬ଷस͈ൎဓ‫ ͍ݞ‬HIV ۜஅ൝̽̀͢ͅȂ྾‫ע‬೩‫ેئ‬ఠ̦ৼ̯ܳͦͥ͂ EBV ͈ࢭુഎ̈́‫̦ا଻ڰ‬ည൵̯ͦͥȃ̷͈‫͉ا଻ڰ‬ΨȜ΅ΛΠςϋΩਆ͞γΐ΅ϋςϋΩਆ൝͈՛଻ςϋΩ‫ݩ‬௩૓৖ۛ͞஠૸଻΀ςΞζΠȜΟΑ͞ఉอ଻ࣁ‫ا‬છ൝͈ুࡨ྾
‫ע‬৖ۛͅ‫۾‬ဓ̳̭̦ͥ͂াऐ̯̞ͦ̀ͥȃِș͉ EBV ͈ஆ໖ۜஅ‫͞ࢹܥ‬਽৽྾‫͈͂ࠏע‬௖ࡽैဥͬ৽֒ͅഥঊ٨་
൲໤ͬဥ̞ٜ̀ଢ଼̱̞̀ͥȃ̹͘Ȃ΄ϋζΰσβΑ;ͼσΑ͈ζ;ΑۜஅκΟσ̱͂̀Ȃ0XULQHȖKHUSHVYLUXV(MHV68) ͬဥ̞̹̦ͭȆ྾‫ע‬ພอછ‫͈ࢹܥ‬ٜྶͬদ͙̞̀ͥȃडਞഎ̭͈ͦͣͅࡄ‫ͬݪ‬೒̲̀Ȃ;ͼσΑۜஅ଼ၛͅ
̤̫ͥ਽৽྾‫۬ע‬ণͬٝ๰̳ͥ໦ঊι΃ΣΒθٜͬྶ̳̭ͥ͂̽̀͢ͅȂ਽৽྾‫ͬ଻৻ୖ͈ע‬ၑٜ̳ͥ͂͂͜ͅȂ
EBV̦͂ͭ‫ࡨু͞ا‬྾‫ע‬৖͈ۛ͂‫۾‬Ⴒٜͬྶ̳̭ͥ͂̽̀͢ͅȂ࢘‫ض‬എ̈́EBV‫۾‬Ⴒ৖͈ۛহၷ࿪‫ٳ‬อͬ࿒ঐ̳Ȫ଎ˏȫȃ
ˍȫ(%9/DWHQWPHPEUDQHSURWHLQ ͥ͢ͅळ༾௩૓Ȇ໦‫ ͂ࢹܥا‬EBV ພఠ͈͒‫۾‬ဓ͈ٜྶȁEBV ۜஅ͉ΪΠ B ळ༾͞ષ๧ळ༾ͅచ̱̀‫̞ޑ‬ळ༾ࠁৗഢ۟‫͂଻ڰ‬ະঘ‫̦̭̳̹͂ͣͬ͜ا‬౶̞ͣͦ̀ͥȃ߃ාȂ
EBV ͉ B ळ༾͞ષ๧ळ༾̴͈͙̈́ͣȂT ळ༾͞ NK ळ༾ۜͅஅ̱Ȃྕ଻‫ڰ‬൲଻ EBV છ࢓߲ࠬ͞‫ݩ‬᭻૙છ࢓ฺ߲ͬ̽
̹๽଻ςϋΩਆ͈อછͅ‫۾‬ဓ̳̭̦ͥ͂াऐ̯̞ͦ̀ͥȃEBV ۜஅͥ͢ͅஆ໖ۜஅ֒ഥঊॲ໤͈ಎ́ /03 ͂Ĳĵ/03D ྄̞̹͂̽ΗϋΩ·͉̭͈ͦͣਆនͅ‫̞ޑ‬อ࡛ͬা̱̤̀ͤȂ̷͈ਆនอ୆ً೾̤̞̀ͅ‫̥ͣة‬࿨‫̱̹ضͬڬ‬B ळ༾̤̞̀ͅ /03 ͉ CD40 ΏΈ΢σ֚ͬ໐࿅༩̱Ȃ̞̀ͥ‫خ‬ෝ଻̦াऐ̯ͦͥȃ༷֚ /03D ͉ B ळ༾ࢯࡔτΓίΗȜΏΈ΢σͬࢭુഎ‫ ̵̭̯́͂ͥا଻ڰ‬B ळ༾ਆនࠁ଼ȂB ळ༾໦‫ا‬વ‫ͬٺ‬ည൵̳̭̦ͥ͂౶̞ͣͦ̀ͥȃ̱̥̱Ȃ̷͈ఈळ༾ਅ̤̫ͥͅਆនࠁ଼ෝȂळ༾௩૓Ȇ໦‫ا‬વ‫ͥ͢ͅٺ‬྾‫ע‬ΏΑΞθ෫౦‫۾̠͈̓͢ͅͅࢹܥ‬ဓ̱̞̥͉̀ͥྶ
̥̯̞̞ͣͦ̀̈́ͅȃ̱̹̦̽̀Ȃ൚ࡄ‫ݪ‬໦࿤͉́ /03/03D ͅ಍࿒̱Ȃळ༾ਅ̹͉͘ळ༾໦‫ا‬౲‫ٴ‬අ։എͅ/03 ͬอ࡛̳ͥζ;Αͬै଼̱Ȃ̷͈྾‫ڠע‬എٜଢ଼ͬদ͙̞̀ͥȃ ଎ː)ȃ

଎ˏȅEBV ͂਽৽྾‫ע‬ΏΑΞθEBV ͈ࠁৗഢ۟‫ۜ͂ࢹܥ‬அ଼ၛ‫͉ࢹܥ‬྾‫ע‬ΏΑΞθ͈ୖ৻଻͂ྟ୪ͅ‫۾‬Ⴒ̱̞̀ͥȃˍȅB ळ༾໦‫ا‬ਘૐȪ਽৽྾‫ע‬ळ༾୆ంΏΈ΢σ͈࿅༩ȫˎȅஅ૗ఘ͈ະհ೰‫ˏا‬ȅ྾‫ע‬۬ণ̥͈ͣٝ๰Ȫஆ໖ۜஅ֒ഥঊ͈ۖ஠ະ‫ا଻ڰ‬ȫ

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଎ːȅ(%9/03/03D ͈ࢹ௮Ƚ/03 ͉
6 ྄ٝ۹೒߿ΗϋΩ·́ळ༾ඤႀ֖ͅ CD40
͂൳အͅ TRAF ࣣࠫႀ֖ͬခ̱ȂNF-kB ͬ‫̳ͥا଻ڰ‬ȃ༷֚Ȃ/03D ͉ 12 ྄ٝ۹೒߿ΗϋΩ·́ȂNȽྎ౤ळ༾ඤႀ֖ͅ 6UF
έ͹ηςȜ‫ ͍ݞ‬6\N ΙυΏϋ΅΢ȜΔ͈͂
ࣣٛ‫ͬ଻ڰ‬ခ̱Ȃ$NW ͬ‫̳ͥا଻ڰ‬ȃˎȫ0XULQHȖKHUSHVYLUXV 0+9 ͬဥ̞̹;ͼσΑȽ਽৽‫ۼ‬௖ࡽैဥȁEBV ͈߃‫ͼ;͈׻‬σΆ̜ͥ MHV68 ͬζ;ᾼࠐ๽ۜஅ̵̯ͥ͂Ȃ֤൮͞ࡤ‫͈́ܕݟ‬௩૓ࢃȂB ळ༾̤̞̀ͅྲ;ͼσΑ͈ॲ୆ฺ̞ͬͩ̈́
ஆ໖ۜஅેఠͅ֊࣐̳ͥȃ̷͈ࢃȂMHV68 ͉Ȃ਽৽͈ఘඤ̷͈ͅ୆‫ͅپ‬̹ͩ̽̀঵௽ۜஅ̳ͥȃ̭͉ͦȂEBV ͈ΪΠ͈͒ۜஅ͂႒য̱̹൲ఠ́
̜ͤȂζ;ᾼۜஅ̱̞̈́ EBV ͈κΟσ̱͂̀ȂMHV68 ͉ခဥ̜́ͥ
͂ࣉ̢ͣͦͥȃ൚ࡄ‫ݪ‬໦࿤͉́ȂMHV68 ۜஅͅ؊൞̳ͥ਽৽͈အș̈́྾‫ע‬฽؊͞྾‫ע‬ພఠอછͬ໦ঊτασ́ၑٜ̳̭ͥ͂ͬ࿒എ̱͂̀ࡄ‫ͬݪ‬ૺ̞͛̀ͥȃ̷͈֚̾ͅȂِș͉஠૸଻΀ςΞζΠȜΟΑȪ6/(ȫͅ಍࿒̱̞̀ͥȃ6/( ͉ EBV ͈͂‫۾‬Ⴒ̦ࡣ̩̥ͣাऐ̯̞̦ͦ̀ͥȂ̷͈อછ‫ܥ‬੬͉ະྶ̜́ͥȃ‫ྙޟ‬૬̞̭͂ͅȂ߃࢐ࠏζ;Ὰ̤̞ͅ MHV68 ۜஅ́ুࡨࢯఘ̦ည൵̯̦ͦͥȂ̭͉ͦȂB ळ༾ঐ࢜଻;ͼσΑ̦ুࡨ฽؊଻ B ळ༾͈੄࡛ͬညอ̳ͥ‫خ‬ෝ଻ͬাऐ̱̞̀ͥȃ࡛हȂ൚ࡄ‫ݪ‬໦࿤͉́ȂMHV68 ۜஅζ;Αဇြ B ळ༾̤̫ͥͅুࡨ฽؊଻͂;ͼσΑۜஅ͈͂௖‫࠿ͬ۾‬൦̱̞̀ͥȃ̷͈ఈȂழ̢۟;ͼσΑ͈ैୋ࣐̞ͬȂΠς၅ฒͺσήηϋࠖ͞࢕ΗϋΩ·ৗͬழࣺ̺ͭ;ͼσΑ̈́̓ͬ‫ैͅܡ‬ୋ̱ ଎ˑȅMHV68 ͬဥ̞̹྾‫ע‬ଷࢄ‫͈ࢹܥ‬ٜྶȽ̞̀ͥȃ̭͈ͦͣ;ͼσΑͬဥ̞̭ͥ͂̽̀͢ͅ;ͼσΑȽ਽৽௖ࡽै ֒ഥঊ٨་ζ;Α͞ς΋ϋΫ΢ϋΠ MHV68 ͬဥȂඅͅȂ྾‫ע‬ΏΑΞθͅ։ુͬခ̳ͥΦΛ·ζ;Α൝͈́ۜஅ৘ࡑͅ ဥ̞̭ͥ͂̽̀͢ͅȂ਽৽྾‫ע‬؊൞Ȃۜஅ൲ఠͥ͢྾‫ע‬؊൞ȂMHV ۜஅ൲ఠ͈‫خ‬ণ‫ا‬Ȃ̯ͣͅࢯࡔඅ։എ̈́ςϋΩ‫خ ͈ݩ‬ণ‫ࡢ͍ݞا‬ఘτασ̤̫ͥͅࢯࡔඅ։എςϋ௩૓Ȇ໦‫̫̤ͥͅا‬ບ‫ث‬ΏΑΞθ൝Ȃin vivo ̤̫ͥͅ਽৽྾‫ע‬؊൞ଷࢄ Ω‫ݩ‬௩૓Ȇ໦‫࠿͈ا‬൦̦‫خ‬ෝ͂̈́ͥȃ‫͈ࢹܥ‬ၑٜ࣓ͅࡃ̳ͥ͂ࣉ̢̞̀ͥ ଎ˑ)ȃड߃͈యນഎ̈́ა໲
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֓‫ڠ‬ฎআȁஜനȁဉ༟੎‫ޗ‬਎Ȫ࠳ȫġ ֓‫ڠ‬ฎআȁఆષȁၻঊ੩‫ޗ‬ġฎআȪၑ‫ڠ‬ȫ൥നȁେ‫ݛ‬අහ੩‫ޗ‬ġฎআȪ֓‫ڠ‬ȫ૰ᚧȁํ࣐εΑΡ·Ȫ࠳ȫġ ֓‫ڠ‬ฎআȁȁؐȁ࿠൭ȁ൚ࡄ‫ݪ‬໦࿤͉́Ȃ୆ఘཡࢄ‫۾̦ࢹܥ‬ဓ̳ͥအș̈́୆໤‫ڠ‬എȂ֓‫ڠ‬എ࿚ఴͬ৾ͤե̞̽̀ͥȃ̩͂ͅȂGPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͈୆଼ࣣȆ࿶௣ࠐႹ͞୨౯Ȇဋၗ‫ݪࡄ͈ࢹܥ‬Ȃ̷͈ͦͣ։ુͥ͢ͅ৖͈ۛພఠȆอછ‫ܥ‬੬ͅ‫ݪࡄ̳ͥ۾‬Ȃळ༾ඤ΂σ΄Υρ͈ pH ಺୯̷͈͂୆ၑഎփ݅ͅ‫̞࣐ͥ̀̽ͬݪࡄ̳ͥ۾‬ȃˍȫGPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͈୆଼ࣣȆ࿶௣ȆςκΟςϋΈ‫ݪࡄ͈ࢹܥ‬ȁGPI ͺϋ΃Ȝ͉ȂγΑέ͹ΙΐσͼΦΏΠȜσͅΈσ΋΍ηϋȂζϋΦȜΑȂ΀ΗΦȜσͺηϋςϋॸ̦ࣣ̱ࠫ
̹ൠডৗ͈֚ਅ̜́ͥȃሶ඿൲໤̷̤̞͉̤̀͢ͅ 150 ਅ႒͈ΗϋΩ·ৗ̦ȂGPI ͺϋ΃Ȝ̱ͬٚ̀ळ༾྄ࣣࠫͅ
̱̤̀ͤȂ̭ͦͣ GPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͉ͅ୆ఘཡࢄ͞ळ༾‫ૂ͈ۼ‬༭ഥోͅਹါ̈́࿨‫͈̞̱̹ͥ̀͜ضͬڬ‬̦ఉ̩‫̥͕ͥͦ͘܄‬Ȃ;ͻσΑ͞ඉள͈਋ယఘ̱͂̀‫ܥ‬ෝ̱̞͈̜̀ͥͥ͜͜ȃ̹͘ GPI ͥ͢ͅਘૐ͉ΗϋΩ·ৗ͈ޫहȆΕȜΞͻϋΈΏΈ΢σ̱͂̀൱̞̞̀ͥȃِș͉઀༾ఘ̤̫ͥͅ GPI ͺϋ΃Ȝ͈୆଼ࣣͅ‫۾‬ဓ̳ͥ PIG
ȪPhosphatidylInositol Glycanȫ֒ഥঊ߲Ȃ઀༾ఘ଼ࣣ̯̹́ͦࢃ GPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͈ΕȜΞͻϋΈȆޫहً೾ͅ‫ ̳͖ݞͬޣג‬PGAPȪPost GPI-Attachment to Proteinsȫ֒ഥঊ߲͈൳೰Ȇ‫ܥ‬ෝٜଢ଼࣐̞ͬ̈́̽̀ͥȪ଎ˍȫȃ̭͈ͦͣࡄ‫ͤ͢ͅݪ‬Ȃ‫࡬ة‬ఉ̩͈ΗϋΩ·ৗ̦ GPI ͺϋ΃Ȝ́ਘૐ̯͈̥ͦͥȂ̷͈୆໤‫ڠ‬എփ݅ͬྶ̥̱ͣͅ
̹̞͂ࣉ̢̞̀ͥȃ଎ˍȇGPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͈୆଼ࣣȆ࿶௣ࠐႹGPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͉Ȃ઀༾ఘ́ GPI
ͺϋ΃Ȝ͂ΗϋΩ·ৗ̦ࣣ̳̭ࠫͥ͂ͤ͢ͅ
୆଼ࣣ̯ͦͥȃ଼ًࣣ೾́βσ΂΅ΏΕȜθဇြ͈ͺσ΅σ߿ডৗ̦ GPI ࣺ৾ͤͦͥ͘ͅȃ
GPI ͺϋ΃Ȝ͈୆଼ࣣ‫͍ݞ‬ΗϋΩ·ৗ͈ࣣ͒ࠫͅ‫۾‬ဓ̳͈͉ͥ PIG ֒ഥঊ߲̜́ͥȃ̷͈ࢃȂΌσΐఘͬࠐ̀ळ༾ນ࿂ͅ࿶௣̯ͦȂρέΠͅෛੀ̯ͦͥȃPGAP ֒ഥঊً߲̦̭͈
೾ͅ‫۾‬ဓ̳ͥȃ࡛हȂ֒ഥঊ൳೰̯̞ͦ̀ͥ
PGAP1ȂPGAP5 ͉ ઀ ༾ ఘ ͅ PGAP2ȂPGAP3
͉Όσΐఘͅంह̱ȂGPI ͺϋ΃Ȝ͈ൠङȆডৗςκΟςϋΈͅ‫۾‬ဓ̱̞̀ͥȃ̭͈ςκΟςϋΈ͉ GPI ͺϋ΃Ȝ͈໤ၑඅ଻ͬ་̢
GPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͈ޫहȂ࿶௣ͅ‫ג‬‫̦̭̳͂ͥޣ‬া̯̹ͦȃˎȫࢃഛ଻ GPI ࠧఅછȪอै଻࿡‫ࠬۼ‬૗ளෂછ PNHȫ͂୶ഛ଻ GPI ࠧఅછ͈อછ‫ܥ‬੬ȁȁPNH ͉ GPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ̜́ͥ༞ఘଷࢄ֦ঊ̦ࠧఅ̱̞̹̀ͥ͛ͅȂ୤ࠬ‫̦ݩ‬༞ఘͤ͢ͅ෫ٟ̯ͦ̀Ȃဣࠬ଻ຫ̧̹̳ࠬͬࠬ‫ס‬৖̜ۛ́ͥȃࢃഛഎͅ௮ࠬۚळ༾͈ GPI ୆଼ࣣͅຈଌ͈֒ഥঊ PIG-A ͅඏட་։̦̤̭ͤ GPI ࠧఅळ༾̹͂̈́̽ࢃȪΑΞΛίˍȫȂ໵อ̳ͥুࡨ྾‫ע‬എ‫ܥ‬੬̥ͣ൪ͦ̀·υȜϋ̦௩ఱ̱ȪΑΞΛίˎȫȂ̯ͣͅਆន଻௩૓ͬြ̳֒ഥঊ་։̦̭̭ܳͥ͂ͤ͢ͅພఠ଼̦̳ۖͥ ( ΑΞΛίˏ ) ͂ࣉ̢̤̀ͤȂ̭͈ΑΞΛίˏͅ‫۾‬ဓ̳ͥ࢓༞֒ഥঊ̱͂̀ HMGA2 ͬ൳೰̱̹Ȫ଎ˎȫȃ࡛ह HMGA2 ֒ഥঊ͈։ਫ਼଻อ࡛͈‫ܥ‬੬ͬྶ̥ͣ
̳̭ͥ͂ͬͅ࿒ঐ̱̞̀ͥȃȁِ̹͘ș͉‫͈࣭ם‬ΈσȜί͈͂‫ވ‬൳ࡄ‫ ͤ͢ͅݪ‬GPI ୆଼ࣣͅຈଌ͈֒ഥঊȂPIGM ͈։ુͥ͢ͅ୶ഛ଻ GPI ࠧఅછͬଲ‫́ٮ‬ই͛̀༭̱̹̦࣬Ȃ̷͈ࢃষଲయΏȜ·΀ϋ΍Ȝͬঀ̹ٜ̽ଢ଼൝ͤ͢ͅȂPIGVȂPIGNȂPIGA ͬୣහĲķ֒ഥঊ̳͂ͥ୶ഛ଻ GPI ࠧఅછ̦ࡉ̥̞̾̽̀ͥȃPIGV ࠧఅછ͉Ȃࣞͺσ΃ςγΑέ͹ΗȜΔࠬછḀ̑̀ͭͭȂ‫׋‬൲ୈ૰อో͈ಁతȂ܏ཞȂ਀ঐ͈։ુͬ৽છે̱͂Ȃਲြ Mabry syndrome ͂࡞̞ͩͦ̀ͥ৖͈ۛୣහ֒ഥঊ͈
̜̭̦֚̾́ͥ͂฻ྶ̱̹ȃȁGPI ͺϋ΃Ȝ߿ΗϋΩ·ৗ͈୆଼ࣣ͂࿶௣ͅ‫֒ͥͩ۾‬ഥঊ͉ 25 ࡢոષ·υȜΣϋΈ̯̤ͦ̀ͤ ( ଎ˍ )Ȃࣽࢃ̭͈ͦͣ֒ഥঊͬୣහ֒ഥঊ̱͂Ȃ൳အ͈છેͬ̾͜୶ഛ଻ GPI ࠧఅછ̦อࡉ̯ͦͥ
‫خ‬ෝ଻͉ఱ̧̩Ȃۛ৪͈ٜଢ଼ͬૺ̭͛ͥ͂ͤ͢ͅ૧̹̈́৖ۛౙպͬ‫ږ‬ၛ̱̹̞͂ࣉ̢̞̀ͥȃ

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଎ˎȇPNH อછ͈ι΃ΣΒθȪκΟσȫࣸଡಎ͈ˍࡢȪȡତࡢȫ͈ࠬ‫ۚס‬ळ༾͈ PIG-A ֒ഥঊͅ་։̦̭ܳͤ GPI ͺϋ΃Ȝ߿ΗϋΩ·ৗͬࠧఅ̳ͥ (STEPˍ )ȃࠬ‫ۚס‬ळ༾ͅచ̳ͥুࡨ྾‫ע‬എ̈́฽؊̦̭ܳͥ͂ GPI ͺϋ΃Ȝࠧఅळ༾̷͉͈ࢲ̥ࠢͣ൪ͦ̀·υȜϋ଻ͅ‫ڐ‬ఱ̳ͥ (STEPˎ )ȃ̯ͣ֒ͅഥঊ་։
̦‫̀̽ͩح‬ၻ଻ਆន଻ͅ௩૓̱ྎઑ͈ࠬఱ໐໦̦։ુळ༾́୸̠͛ͣͦͥ̈́ͥ͢ͅ (STEPˏ)ȃ

ˏȫळ༾ඤॸ଻΂σ΄Υρ͈ pH ಺୯̷͈͂୆ၑഎփ͈ٜ݅ྶ
ȁळ༾ඤ΂σ΄Υρ͉ডৗඵਹ྄̤̽̀͢ͅࡽ̞ͅ‫ڞ‬ၗ̯̞ͦ̀ͥ
ْ໦̜́ͤȂ̷͈‫ܥ‬ෝͬडఱࡠͅอ‫ࡥ̹̳͛ͥͅܞ‬ခ͈۪‫͞ޏ‬Ηϋ
Ω·ৗȆডৗ଼໦ͬ༗঵̱̞̀ͥȃ̷͈ਹါ۪̈́‫֦ޏ‬ঊ͈̦֚̾
pH ̜́ͥȃ໦๥ࠐႹ͞΀ϋΡ΍ͼΠȜΏΑࠐႹ͈΂σ΄Υρ͈ඤ͉࣌ࡥခ͈ॸ଻ pH ͅ༗̹̞ͦ̀̀Ȫ଎ˏȫȂ̷͈։ુ́ळ༾τασ͉́ȂΗϋΩ·ৗȆডৗ͈࿶௣ȆίυΓΛΏϋΈȆൠङਘૐવ‫ٺ‬͞΂σ΄Υρ͈ࠁఠ։ુ̦Ȃ̹͘ࡢఘτασ͉́အș̈́৖̦ۛอછ
̳̭̦ͥ͂౶̞ͣͦ̀ͥȃ̷͈ਹါ଻͜ͅࢰ̴̷͈ͣͦͣ։ુέͿΦΗͼί͈̭ܳͥι΃ΣΒθ̞͉͕̾̀͂ͭ̓ͅ฻̞̞̽̀̈́ȃ൚ΈσȜί͉ȂΌσΐ௡౾͈ pH ։ુͬা̳་։ळ༾‫ͬڼ‬੝͛̀ਏၛ
̱Ȃ̷͈ୣහΗϋΩ·ৗ͈൳೰Ȇ‫ܥ‬ෝٜଢ଼ͬ༭̱̹࣬Ȫ໲ࡃˑȫȃࣽࢃȂ̭͈ͦͣ་։ळ༾Ȇ֒ഥঊ͈၌ဥ໼͍ͅ૧̹̈́ pH ։ુͬা̳་։ळ༾‫͈ڼ‬ਏၛٜଢ଼ͬ೒̲̀Ȃळ༾ඤ΂σ΄Υρ͈ pH ͈಺୯‫̷͈͞ࢹܥ‬୆ၑഎփ̞݅̾̀ͤ͢ͅၑٜͬ૬͛ȂpH ։ુ̦ພ֦̜́ͥ৖ۛͅచ̳ͥ૧̹̈́஻࿪͈‫ٳ‬อ͈͒ඏ෫࢛̭͂̈́ͥ͂ͬ
࿒എ̱̞͂̀ͥȃ଎ˏȇळ༾ඤ΂σ΄Υρ͈ pH ಺୯໦๥ࠐႹ͞΀ϋΡ΍ͼΠȜΏΑࠐႹ͈΂σ΄Υρඤ̷̸͉࣌ͦͦࡥခ͈ॸ଻ pH ͅ಺୯̯̞ͦ̀ͥȃତল̷͉̤̤͈͢ pH ͬা̳ȃड߃͈యນഎ̈́ა໲
1. Murakami Y, Kanzawa N, Saito K, Krawitz PM, Mundlos S, Robinson PN, Karadimitris A, Maeda Y, Kinoshita T.
Mechanism for release of alkaline phosphatase caused by glycosylphosphatidylinositol deficiency in patients withhyperphosphatasia mental retardation syndrome. J Biol Chem. 2012 Feb 24;287(9):6318-25.
2. Fujita M, Watanabe R, Jaensch N, Romanova-Michaelides M, Satoh T, Kato M, Riezman H, Yamaguchi Y, Maeda
Y, Kinoshita T. Sorting of GPI-anchored proteins into ER exit sites by p24 proteins is dependent on remodeled GPI.
J Cell Biol. 2011 Jul 11;194(1):61-75.
3. Kanzawa N, Maeda Y, Ogiso H, Murakami Y, Taguchi R, Kinoshita T. Peroxisome dependency of alkyl-containing
GPI-anchor biosynthesis in the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17711-6.
4. Fujita M, Maeda Y, Ra M, Yamaguchi Y, Taguchi R, Kinoshita T. GPI glycan remodeling by PGAP5 regulates
transport of GPI-anchored proteins from the ER to the Golgi. Cell. 2009 Oct 16;139(2):352-65.
5. Maeda Y, Ide T, Koike M, Uchiyama Y, Kinoshita T. GPHR is a novel anion channel critical for acidification andfunctions of the Golgi apparatus. Nat Cell Biol. 2008 Oct;10(10):1135-45.
Ĳĸࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities୆ఘཡࢄࡄ‫ݪ‬໐࿝ুட྾‫ڠע‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎Ȫ࠳ȫġ֓‫ڠ‬ฎআȁૣၻȁ୓౳੎‫ޗ‬਎ġ
֓‫ڠ‬ฎআȁ‫ࣣع‬ȁఊ჊අහ੎‫ޗ‬਎Ȫ࠳ȫġ ֓‫ڠ‬ฎআȁ૒ઐȁ౺੩‫ޗ‬ġ֓‫ڠ‬ฎআȁḶ൥ȁోढඅහ੩‫ޗ‬ġ֓‫ڠ‬ฎআȁߧ౓ȁဎఊ჊අහ੩‫ޗ‬Ȫ࠳ȫġ ֓‫ڠ‬ฎআȁऎ൥ȁ௝ȁুட྾‫͉ע‬ळ޿͞;ͼσΑ̞̹͂̽ۜஅພࡔఘ͈੝‫෇ܢ‬ে̷͍͈̈́ͣͅࢃ͈‫׶‬છ฽؊͈ৼܳ͞‫ڕ‬ං྾‫͈ע‬ည൵ͅਹါ̈́࿨‫̞̱̹ͥ̀ضͬڬ‬୆ఘཡࢄι΃ΣΒθ̜́ͥȃ̷͈༷֚Ȃুட྾‫͈ע‬։ુ͉Ȃ྾‫ע‬ະ஠Ȃ෴ࠬછ଻ΏοΛ·Ȃুࡨ྾‫ע‬৖ۛ̈́̓အș̈́৖͈ۛࡔ֦͂̈́ͥȃুட྾‫ͬע‬ংͥळ༾̜́ͥζ·υέ͹Ȝΐ͞ਏેळ༾͉ພࡔఘࡥခͅ
ంह̳ͥࢹ௮ȪPathogen-associated molecular patterns:PAMPsȫͬ෇ে̳ͥΩΗȜϋ෇ে਋ယఘȪPattern-recognition
receptors:PRRsȫͬอ࡛̱̞̀ͥȃPRRs ̦ PAMPs ͬ෇ে̳ͥ͂‫ا଻ڰ‬ΏΈ΢σ̦ഥో̯ͦȂ֚Ⴒ͈ুட྾‫ע‬؊൞̦ည൵̯ͦͥȃ൚ࡄ‫ݪ‬໦࿤͉́Ȃুட྾‫ͥ͢ͅע‬୆ఘཡࢄଷࢄι΃ΣΒθ̞͈̾̀ͅࡄ‫̞࣐ͥ̀̽ͬݪ‬ȃˍȫPRRs ̳ͬٚͥૂ༭ഥోࠐႹͅ‫ͥͩ۾‬૧ܰ໦ঊ͈ౝ॑͂࿨‫͈ڬ‬ٜଢ଼ȁPRRs ̱͂̀ȂToll-like receptors (TLRs)ȂRIG-I-like receptors (RLRs)ȂNod-like receptors (NLRs) ̦౶̞ͣͦ̀ͥȃِș͉Ȃ໦ঊ୆໤‫ڠ‬എ਀༹͞Ψͼ΂ͼϋέ΁ζΞͻΛ·Αͬဥ̞̀ PRRs ͞‫ئ‬ၠૂ༭ഥోͅպ౾̳ͥ໦ঊ͈ౝ࣐̠॑ͬ
͂͂͜ͅȂΦΛ·ͺ;Πζ;Αͬैୋ̱Ȃ྾‫ע‬؊൞̤̫ͥͅ࿨‫̞̱̠̱̥ͥ̀͂ͣ͢ͅྶͬڬ‬ȃಎ́͜Ȃ;ͼσΑ͞ळ޿͈̾͜‫ͅॸړ‬ಕ࿒̱Ȃুட྾‫෇ॸړͥ͢ͅע‬েι΃ΣΒθͬྶ̥̱̠̱̞ͣ͂̀ͥ͢ͅȪ଎ˍȫȃ଎ 1ȁ‫෇ॸړ‬েͅ‫ͥͩ۾‬਋ယఘ͂ૂ༭ഥోࠐႹ
‫෇ॸړ‬েͅ‫ ͥͩ۾‬TLR3ȂTLR7ȂTLR9 ͉৽ͅ΀ϋΡΕȜθͅޫह̱̞̀ͥȃTLR3ȂTLR7Ȃ˰˨ˮ
9 ̷̸͉ͦͦ;ͼσΑဇြඵਹङ RNAȂRNA ;ͼ
σΑུ͈֚ङ RNAȂDNA ;ͼσΑ͞ळ޿͈̾͜
DNA ͬ෇ে̳ͥȃTLR3 ͉ TRIFȂTLR7 ͞ TLR9
͉ MyD88 ͬͺΘίΗȜ̱̱͂̀ٚ I ߿ͼϋΗȜέͿυϋ͞‫׶‬છ଻΍ͼΠ΃ͼϋॲ୆ͬည൵̳ͥȃ༷֚Ȃळ༾ৗඤ͉́ RLR (RIG-IȂMDA5ȫ̦ RNA;ͼσΑဇြ͈ RNA ͬ෇ে̱ȂͺΘίΗȜ IPS-1
̱ͬٚ̀ૂ༭ഥోͬ‫ٳ‬ই̳ͥȃ̹͘ȂDNA ෇েͅ‫ͥͩ۾‬ळ༾ඤΓϋ΍Ȝ̱͂̀ AIM2 ̦౶̞ͣͦ̀
ͥȃAIM2 ͉ͺΘίΗȜ ASC ̱ͬٚ̀৽ͅ ,/ȕ͈ॲ୆ͅ‫۾‬ဓ̳ͥȃ̧̺͉̱̞̞̦̽ͤ̀̈́͘Ȃ
STING ͂ࡤ͊ͦͥ໦ঊ͈ષၠ͉ͅ I ߿ͼϋΗȜέͿυϋॲ୆ͅ‫۾‬ဓ̳ͥ‫͈̥ͣة‬ळ༾ඤ DNA Γϋ΍Ȝ̦ంह̱̞̭̦̀ͥ͂াऐ̯̞ͦ̀ͥȃˎȫιϋήτϋΠρέͻΛ·ଷࢄ໦ঊͥ͢ͅুட྾‫ע‬؊൞ଷࢄ‫͈ࢹܥ‬ٜଢ଼ȁιϋήτϋΠρέͻΛ·͉ PRRs ̱̹ͬٚুட྾‫ע‬؊൞ͅ૬̩‫̦̭̞͂ͥ̀̽ͩ۾‬ఉ̩༭̯̞࣬ͦ̀ͥȃِș͉Ȃ΂ȜΠέ͹ΐȜ‫۾‬Ⴒ໦ঊ ATG16L1ȂATG9a ͞ͼϋΗȜέͿυϋည൵໦ঊ Viperin ͅ಍࿒̱Ȃ̭ͦͣ໦ঊ̦ PRRs ͥ͢ͅ‫׶‬છ଻΍ͼΠ΃ͼϋ͞˥߿ͼϋΗȜέͿυϋॲ୆ͬଷࢄ̱̞̭̀ͥ͂ͬྶ̧̥̱̹ͣ̀ͅȃ̭ͦͣͬ௷̦̥ͤ
̱͂̀Ȃ྾‫ע‬؊൞̤̫ͥͅιϋήτϋΠρέͻΛ·͈࿨‫͈̞̀̾ͅڬ‬મळͬྶ̥̱̠̱̞ͣ͂̀ͥ͢ͅȃ
3ȫুட྾‫ע‬ळ༾̤̫ͥͅഢৢଷࢄ֒͞ഥঊอ࡛ΥΛΠχȜ·͈ٜଢ଼ȁအș̈́ΦΛ·ͺ;Πζ;Α͈ਏેळ༾̤̫ͥ֒ͅഥঊอ࡛ͬ࿌္എͅ๤‫ڛ‬Ȇٜଢ଼̱ȂίυκȜΗȜ͈ࢹ௮ͬ಺͓
̭ͥ͂́ഢৢ֦ঊΥΛΠχȜ·̦͈̠̱̓̀͢ͅດഎ֒ഥঊ͈อ࡛ଷࢄ࣐̞̥ͬ̽̀ͥͬၑٜ̳̭ͥ͂ͬ࿒ঐ̱̀
̞ͥȃΪΑΠϋਘૐ൝͈ٜଢ଼࣐̞͜Ȃুட྾‫ע‬؊൞͞ুட྾‫ע‬ळ༾໦‫ا‬Ȫඅͅ M1Ȃ˩2 ζ·υέ͹Ȝΐ໦‫ا‬ȫ̤̫ͥͅ΀άΐͿΥΞͻΛ·̈́ଷࢄ͈࿨‫̞̱̠̱̥ͥ̀͂ͣ͢ͅྶ͜ڬ‬Ȫ଎ˎȫȃĲĹ଎ 2ȁJmjd3 ͥ͢ͅ M2 ߿ζ·υέ͹Ȝΐ͈‫ࠐا଻ڰ‬Ⴙ
ζ·υέ͹Ȝΐ͉‫ܥ‬ෝഎͅ։̈́ͥ M1 ͂ M2 ߿ͅ໦̧̫̭̦ͥ͂́
ͥȃM1 ߿ζ·υέ͹Ȝΐ͉৽ͅळ޿Ȅ;ͼσΑ͞૯޿႒͈ۜஅশͅ
‫༷̳֚ͥا଻ڰ‬ȂM2 ߿ζ·υέ͹Ȝΐ͉‫ܙ‬୆ಖۜஅȄͺτσΆȜ؊൞Ȅডཛయ৫Ȅ஻ੱহ࿴Ȅ̦ͭഢ֊̈́̓ͅ‫۾‬ဓ̱̞̀ͥȃJmjd3 ͉ΪΑΠϋ H3 ͈ΠςιΙσ‫ ̹̯ͦا‬27 ๔࿒͈ςΐϋॼ‫౎͈ܖ‬ιΙσ‫ا‬ࣖள̜́ͤȂζ·υέ͹Ȝΐ̤̞̀ͅ TLR ঁࠣ́อ࡛ည൵̯ͦͥȃ
Jmjd3 ̦ࠧఅ̱̹ેఠ͉́ȂM-CSF ঁࠣȄ΅ΙϋൎဓȄ‫ܙ‬୆ಖۜஅ͈ष͈ M2 ߿ζ·υέ͹Ȝΐ͈͒໦‫̞̭̦̈́ͣܳا‬ȃ̹͘ȂJmjd3 ͥ͢ͅ౎ιΙσ‫͈ا‬ೄ୪͈ΗȜΊΛΠ͈͉֚̾ IRF4 ֒ഥঊ̜́ͥȃ̭͈̭̥ͦͣ͂ͣȂJmjd3-IRF4 ࠐႹ͉‫ܙ‬୆ಖۜஅͅచ̳ͥ୆ఘཡࢄ؊
൞ͅਹါ̈́࿨‫̢ͥͦͣࣉ̞̱̹͂ͥ̀ضͬڬ‬ȃ

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4) ‫׶‬છ଻΍ͼΠ΃ͼϋ̈́̓ͬ΋ȜΡ̳ͥ mRNA ͈հ೰଻ଷࢄͅ‫ͥͩ۾‬໦ঊέ͹ηςȜ͈‫ܥ‬ෝٜଢ଼ȁ‫׶‬છ଻΍ͼΠ΃ͼϋ͞‫׶‬છ‫۾‬Ⴒ໦ঊ͉ഢৢͅ‫ ̢ح‬mRNA հ೰଻͈ଷࢄͤ͢ͅอ࡛τασ̦ഐ୨ͅ಺୯̯̞ͦ̀
ͥȃِș͉ TLR ͤ͢ͅอ࡛̦ည൵̯ͦͥ CCCH ߿ Zinc finger ౩ฒৗ Regnase-1 (Zc3h12a) ̦ IL-6 ͞ IL-12p40 ̞̹͂̽‫׶‬છ଻΍ͼΠ΃ͼϋ͈ mRNA ͬ໦ٜ̳ͥ RNase ̱͂̀‫ܥ‬ෝ̱̞̭̀ͥ͂ͬྶ̧̥̱̹ͣ̀ͅȃ̭͈໦ঊͬࠧఅ
̳ͥζ;Α͉ਹඈ̈́ুࡨ྾‫ע‬৖ۛͬুடอછ̳ͥȪ଎ˏȫȃ࡛हȂZc3h12a ̷͈͞έ͹ηςȜ໦ঊͥ͢ͅ‫׶‬છ‫۾‬Ⴒ֒ഥঊ͈ഢৢࢃ಺୯ι΃ΣΒθͅ‫̞࣐ͥ̀̽ͬݪࡄ̳ͥ۾‬ȃ

଎ 3ȁRegnase-1 ͥ͢ͅ‫׶‬છ؊൞ଷࢄ
Regnase-1 ͉ RNase ̜́ͤȂIL-6 ͈̈́̓‫׶‬છ଻΍ͼΠ΃ͼϋ͈֒ഥঊ mRNA ͈ະհ೰‫ͬا‬ଷࢄ̱̀‫׶‬છ଻৖ۛอછͬဲଷ̱̞̀
ͥȃӱࢭુഎͅอ࡛̱̞̀ͥ Regnase-1 ͉ྫঁࠣેఠ͉́ IL-6
mRNA ͬ໦ٜ̱̞̀ͥȃӲIKK ໝࣣఘ̦ TLR ঁࠣͤ͢ͅ‫ا଻ڰ‬̳ͥ͂Ȃ,ț%Į͂ Regnase-1 ͉ςϋॸ‫ͬا‬਋̫໦ٜ̯ͦͥȃӳ1)ț% ‫ ͤ͢ͅا଻ڰ‬IL-6 mRNA ͉ഢৢ̯ͦȂӴհ೰‫ ̞̱ͥ̀ا‬IL-6
mRNA ̽̀͢ͅ IL-6 ̦ॲ୆̯ͦͥȃӵRegnase-1 mRNA ̦ഢৢ̯ͦȂRegnase-1 ΗϋΩ·ৗ̦อ࡛̳ͥȃӶRegnase-1 ΗϋΩ·ৗ͉ळ༾ඤ͈ IL-6 mRNA ͬ໦ٜ̱ȂIL-6 ͈ॲ୆ͬဲଷ̳ͥȃड߃͈యນഎ̈́ა໲
1. Iwasaki H, Takeuchi O, Teraguchi S, Matsushita K, Uehata T, Kuniyoshi K, Satoh T, Saitoh T, Matsushita M,
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7/5,/5E\FRQWUROOLQJGHJUDGDWLRQRIUHJQDVHNat Immunol. 2011 Oct 30;12(12):1167-75.
2. Saitoh T, Satoh T, Yamamoto N, Uematsu S, Takeuchi O, Kawai T, Akira S. Antiviral protein Viperin promotes
Toll-like Receptor 7- and Toll-like Receptor 9-mediated type I interferon production in plasmacytoid dendritic cells.
Immunity. 2011 Mar 25;34(3):352-63.
3. Kawai T, Akira S. Toll-like Receptors and their crosstalk with other innate receptors in infection and immunity.
Immunity. 2011 May 27;34(5):637-50.
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Ĳĺࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities୆ఘཡࢄࡄ‫ݪ‬໐࿝ळ༾‫ܥ‬ෝ໦࿤
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁȁ֓‫ڠ‬ฎআȁ࿒‫ح‬നȁ‫ם‬༟੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ‫ུ܊‬ȁၮ੩‫ޗ‬ȁȁၑ‫ڠ‬ฎআȁକോȁۘ૽අහࡄ‫֥ݪ‬ȁΨͼ΂΍ͼ΀ϋΑฎআȁಎఆȁં঎ȁඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁȁȁȁȁȁȁກനȁ౺࿟ȁඅහࡄ‫֥ݪ‬ȁΨͼ΂΍ͼ΀ϋΑฎআȁऎ൥ȁ̴͙͕ȁ൚ࡄ‫ݪ‬໦࿤͉́Ȃळ༾‫ͅۼ‬ంह̳ͥळ༾௩૓֦ঊ͞ळ༾୪಍֦ঊ̱̹ͬٚळ༾͈‫ܥ‬ෝଷࢄ‫ͬݪࡄ̞̀̾ͅࢹܥ‬࣐̞̽̀ͥȃࡄ‫৽͈ݪ‬࿨͂̈́ͥ໦ঊ͉ȂHB-EGF ̞̠͂ EGF έ͹ηςȜ྄͈ࣣࠫ߿ळ༾௩૓֦ঊ͂ΞΠρΑΩΣ
ῧࡤ྄͊ͦͥːٝ۹೒߿ΗϋΩ·ৗ̜́ͥȃ̭͈ͦͣΗϋΩ·ৗ͉Ȃळ༾‫ٸ‬ζΠςΛ·Α໦ঊ̷͈͞ఈ྄ΗϋΩ·ৗȂ̜̞͉ͥळ༾ඤΏΈ΢σ໦ঊ͂ໝࣣఘͬࠁ଼̱̀Ȃळ༾௩૓͈಺୯Ȃࠁఠࠁ଼͞ழ૕͈֋঵Ȇਘ໘ͅ൱̞̀
̞ͥ͂൳শͅȂ̦ͭळ༾͈௩૓Ȇ૫੕Ȇഢ֊͜ͅ૬̩‫̞ͥ̀̽ͩ۾‬ȃˍȫHB-EGF ͈࿨‫ै͂ڬ‬ဥ‫͈ࢹܥ‬ٜଢ଼ȁHB-EGF ͉ EGF έ͹ηςȜ͈௩૓֦ঊ́ȂEGFR ͞ ErbB4 ࣣ̱ࠫͅȂ̭ͦͣͬ‫̳ͥا଻ڰ‬ȃHB-EGF ྄͉۹೒Ριͼϋͬ‫߿྄ࣣ̺ࠫͭ܄‬ळ༾௩૓֦ঊ଼̱ࣣ̯͂̀ͦȂ྄ࣣࠫ߿̦ळ༾ນ࿂́ίυΞͺȜΔ̽̀͢ͅ୨౯̯ͦ
ͥ͂Ȃ໦๥߿ HB-EGF ͬ୆̲ͥȃHB-EGF ͉Ȃਅș͈ழ૕Ȃळ༾ͤ͢໦๥̯ͦȂ૤௫‫ܥ‬ෝ֋঵͞૤௫༕ࠁ଼Ȃ࿒‫଼ࠁڀ‬Ȃ஻ੱহ࿴Ȃ෹༾ࠁ଼Ȃ਋ୈ၅͈಍઄Ȃນ๧๬ً࢚͈̈́̓೾̤̞̀ͅȂळ༾͈୆ంȂ௩૓ဲଷȂ֊൲Ȃ୪಍Ȃ௩૓௯ૺ̈́̓ఉद̈́‫ܥ‬ෝͬอ‫̞̱ͥ̀ܞ‬ȃ୆ఘඤً͈͕͈͂ͭ̓೾͉́໦๥߿ HB-EGF ̦‫ܥ‬ෝ̱̞̀ͥȃ྄̱̥̱ࠫ
ࣣ߿͉໦๥߿͈ஜߐఘ̜̥̩́ͥ͊ͤ́̈́Ȃ྄ࣣࠫ߿͈ેఠ́͜୆໤‫ͬ଻ڰ‬঵̞̭̥̽̀ͥ͂ͣȂ྄ࣣࠫ߿͈൱
̩୆ၑً೾͜ంह̳ͥ‫خ‬ෝ଻̦̜ͥȃ྄߿̥ͣ໦๥߿͈͒ഢ͉͈̠۟̓͢ͅଷࢄ̯̞͈̥ͦ̀ͥȂ྄߿͂໦๥߿
͈୆ၑഎ࿨‫ڬ‬Ȃ͈̠̓̈́͢‫́ࢹܥ‬ఉद̈́୆ၑ‫ͬ଻ڰ‬া̳͈̥Ȃ̯͉ͣͅພ‫ͤͩ۾͈͂ܨ‬൝͈࿚ఴͅ‫ͬݪࡄ̱̀۾‬ૺ̞͛̀ͥȃ
଎ˍȁ૤௫༕ࠁ଼̤̫ͥͅ HB-EGF ͈࿨‫ڬ‬૤௫༕͈ࠁ଼ً೾́ HB-EGF ͉૤ඤ྄ષ๧ͤ͢໦๥̯ͦȂ༕‫ۼ‬ৗळ༾ͅచ̷̱͈̀௩૓ͬဲଷ̳ͥ൱̧̳ͬͥȪऒ‫ئ‬଎ȫȃ̹͘༕‫ۼ‬ৗ͉ͅΰΩρϋၤॸίυΞ΂Ές΃ϋȪHSPGsȫ̦ཅີͅంह̱Ȫֲષ଎ȫȂ༕‫ۼ‬ৗळ༾ͅచ̱̀ HB-EGF ̦ୃુͅैဥ̳̹͉ͥ͛ͅȂ
HB-EGF ͉ HSPGs ࣣ̳͂ࠫͥຈါ̦̜ͥȃ̱̹̦̽̀ HB-EGF ࠧఅζ;Α (KO) ̺̫̩́̈́ȂHB-EGF ͈ΰΩςϋࣣࠫΡιͼϋ̩ͬࠧ་։߿ HB-EGF ͬอ࡛̳ͥΦΛ·ͼϋζ;Α ǻ+% ͉́Ȃ༕‫ۼ‬ৗळ༾ً͈௩૓̦̭ܳͤȂ૤௫༕̦։ુͅ๬࢚̱̹ࠁఠ̈́ͥͅȪֲ‫ئ‬଎ȫȃ(Iwamoto et al, 2010)ĳı

ˎȫ̦͈ͭ՛଻‫ ̫̤ͥͅا‬HB-EGF ͈࿨‫ڬ‬ȁHB-EGF ͉‫܈‬ळ༾͞‫܈‬ਔս͈‫ۼ‬ৗ́‫̩ޑ‬อ࡛̱Ȃ̦͈ͭ՛଻‫ͅا‬૬̩‫̞ͥ̀̽ͩ۾‬ȃ̦ͭळ༾͈௩૓Ȃ૫੕Ȃഢ֊̤̫ͥͅ HB-EGF ͈‫̳̹ض‬࿨‫ͬڬ‬મ̱̩ٜଢ଼̱Ȃ૧̹̈́‫܈‬হၷ༹͈‫ٳ‬อͬ࿒ঐ̱̞̀ͥȃ

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‫ڰ‬
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଎ˏȁ୆ఘͼιȜΐϋΈͥ͢ͅ‫܈‬ळ༾͈௩૓ͅచ̳ͥ஌
֋ٔळ༾͈࢘‫ض‬ٜଢ଼ȃȪaȫ‫܈‬ळ༾͂஌֋ٔळ༾ͬ൳শ֊
૒̱̹ાࣣȂ
Ȫbȫ‫܈‬ळ༾ͬౙඊ́֊૒̱̹ાࣣ (Murata etal, 2011)
଎ˎȁঊ‫ݠ‬ᴢ‫܈̫̤ͥͅ܈‬ळ༾͂‫ۼ‬ৗ஌֋ٔळ༾͈௖ࡽैဥ

ˏȫHB-EGF ͬ໦ঊດഎ̳͂ͥࢯ‫܈‬स͈‫ٳ‬อͬଔૺ̱ȂHB-EGF ಎგࢯఘ͞ΐέΞςͺඉள་։ఘ CRM197 ͬခ଼࢘໦̳͂ͥ၅௏̦ͭহၷ࿪͈๱ႉ઄দࡑȆႉ઄দࡑͬ৘ঔ̱̞̀ͥȃːȫΞΠρΑΩΣϋ໦ঊ͈ٜଢ଼
ȁΞΠρΑΩΣϋ͉Ȃඅಭഎ྄̈́ːٝ۹೒ࢹ௮ͬ঵̻Ȃఉळ༾୆໤̺̫ͅంह྄̳߲͈֚ͥΗϋΩ·ৗέ͹ηςȜ́ȂΪΠ͉́ˏˌਅ႒ոષȂΏο;ΐο;Ψ΀͞஌ಖ́͜ˎˌਅ႒ոષంह̳ͥȃ஌ಖͬဥ̞̀ΞΠρΑΩΣϋ͈໦ঊ‫ܥ‬ෝͬ֒ഥ‫ڠ‬എٜͅଢ଼̱̞̀ͥȃ଎ːȁ၅௏‫ࣞ́܈‬อ࡛̳ͥ HB-EGF( ऒ ) ͂‫ٳ‬อಎ͈၅௏‫܈‬হၷ࿪ BK-UMȪֲȫड߃͈యນഎ̈́ა໲
1. Miyamoto S, Iwamoto R, Furuya A, Takahashi K, Sasaki Y, Ando H, Yotsumoto F, Yoneda T, Hamaoka M, Yagi H,
Murakami T, Hori S, Shitara K, Mekada E. A novel anti-human HB-EGF monoclonal antibody with multiple
anti-tumor mechanisms against ovarian cancer cells. Clin Cancer Res. 2011 Nov 1;17(21):6733-41.
2. Murata T, Mizushima H, Chinen I, Moribe H, Yagi S, Hoffman RM, Kimura T, Yoshino K, Ueda Y, Enomoto T,
Mekada E. HB-EGF and PDGF mediate reciprocal interactions of carcinoma cells with cancer-associated fibroblasts
to support progression of uterine cervical cancers. Cancer Res. 2011 Nov 1;71(21):6633-42.
3. Iwamoto R, Mine N, Kawaguchi T, Minami S, Saeki K, Mekada E. HB-EGF function in cardiac valve development
requires interaction with heparan sulfate proteoglycans. Development. 2010 Jul;137(13):2205-14.
4. Ichise T, Adachi S, Ohishi M, Ikawa M, Okabe M, Iwamoto R, Mekada E. Humanized gene replacement in mice
reveals the contribution of cancer stroma-derived HB-EGF to tumor growth. Cell Struct Funct. 2010;35(1):3-13.
5. Mizushima H, Wang X, Miyamoto S, Mekada E. Integrin signal masks growth-promotion activity of HB-EGF in
monolayer cell cultures. J Cell Sci. 2009 Dec 1;122(Pt 23):4277-86.
ĳĲࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities୆ఘཡࢄࡄ‫ݪ‬໐࿝྾‫ڠاע‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁ֓‫ڠ‬ฎআȪ࠳ȫġ ࣏ଳȁં੩‫ޗ‬ȁ֓‫ڠ‬ฎআġྎ‫ז‬ȁ಑ࢩ
੩‫ޗ‬ȁ෠‫ڠ‬ฎআġࣝ५ȁٗঊඅහ੩‫ޗ‬ȁȁ༗࠲‫ڠ‬ฎআȁ໹հȁࢭࢨඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁȁ᠟൥ȁঃႹȁඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁȁؐȁ୓ȁ൚ࡄ‫ݪ‬໦࿤͉́Ȃພࡔఘ̦͈̠̓͢ͅ྾‫ע‬ΏΑΞθ̥͈ͣ൪๰‫ڕͬࢹܥ‬ං̧̱̹͈̥̀Ȃ༷֚Ȃ྾‫ע‬ΏΑΞθ͉Ȃਅș͈ۜஅછͅచ̱͈̠̀̓͢ͅ೷ࢯ଻ͬ‫ڕ‬ං̧̱̹̥̞ٜ̀̾̀ͅྶ࣐̞ͬ̽̀ͥȃඅͅȂ྾‫ע‬ळ༾̦อ࡛̳ͥ
ਅș͈‫ا଻ڰ‬ଷࢄτΓίΗȜ͈‫ܥ‬ෝٜଢ଼ͬ೒̱̀Ȃਅș͈ພࡔఘͅచ̳ͥ୆ఘཡࢄ‫͈ࢹܥ‬ٜྶͬ࿒ঐ̱̞̀ͥȃِș͈ࡄ‫ͤ͢ͅݪ‬Ȃఉ̩͈྾‫ע‬ळ༾͈อ࡛̳ͥဲଷ‫̥ͥ̈́ͣا଻ڰ͂ا‬βͺ߿τΓίΗȜ̦Ȫ଎ ĲȫȂພࡔఘ͂‫ૺͅވ‬‫̧̹̱̀ا‬τΓίΗȜ̜́ͥ‫خ‬ෝ଻͞Ȃ̯ͣͅȂ̭͈ͦͣτΓίΗȜ̦;ͼσΑ͈΀ϋΠςȜ͜ͅ၌ဥ̯̭ͦͥ͂
̦ྶ̧̥̹ͣ̈́̽̀ͅȃ̷̭́Ȃ̭͈ͦͣτΓίΗȜͅచ̳ͥ਽৽ς΄ϋΡ͞ພࡔఘς΄ϋΡ͈ٜྶͤ͢ͅȂ਽৽͈ۜஅ೷ࢯ଻͈̠͂̓̈́͢‫̥̜̦ͬͥ߸۾‬ٜྶ̳ͥȃུࡄ‫͉ݪ‬Ȃພࡔఘ͈྾‫ע‬൪๰‫͞ࢹܥ‬૟ව‫͈ࢹܥ‬ٜྶ͞୆ఘ͈
ۜஅ೷ࢯ଻ࠨ೰֦ঊ͈ٜྶͅਹါ̜́ͤȂχ·Ιϋ‫ٳ‬อۜ͞அછထཡ༹Ȃহၷ༹‫ٳ‬อ͈̹͈͛‫ܖ‬யࡄ‫ͥ̈́ͅݪ‬ȃȪĲĪ βͺ߿τΓίΗȜ͈෇ে‫͈ࢹܥ‬ٜྶȁ྾‫ע‬ळ༾͉Ȃဲଷ‫ا଻ڰ͂ا‬τΓίΗȜ଼̥ͣͥਅș͈βͺ߿τΓίΗȜͬอ࡛̱̞̀ͥȪ଎ˍȫȃဲଷ‫ا‬βͺ߿τΓίΗȜ͉ȂŎŉń ൝͈ুࡨ໦ঊͬ෇ে̳༷֚ͥȂ‫ا଻ڰ‬τΓίΗȜ͉ুࡨ໦ঊͬ෇ে̱̞̈́ȃ൚໦࿤͉́Ȃβͺ߿τΓίΗȜ͈̦֚̾΍ͼΠι΄υ;ͼσΑ͈;ͼσΑ໦ঊͬ෇ে̳̭ͥ͂ͬྶ̥̱ͣͅȂβͺ߿τΓίΗȜͥ͢ͅພࡔఘ෇ে̦Ȃۜஅ೷ࢯ଻ͬࠨ೰̳ͥષ́ਹါ̈́‫ܥ‬ෝͬ౜̞̭̽̀ͥ͂ͬྶ̥̱̹ͣͅȪ଎ˎȫȃ̷̭́Ȃ
;ͼσΑ͉̲ͬ͛Ȃࡔಖ͞ळ޿൝͈အș̈́ພࡔఘͬດഎ̱̀ͅȂ̭͈ͦͣβͺ߿τΓίΗȜ̦‫෇ͬة‬ে̱Ȃ͈̠̓̈́͢‫ܥ‬ෝͬ঵̞̥̽̀ͥͬྶ̥̳̭ͣͥ͂ͤ͢ͅͅȂβͺ߿τΓίΗȜ͈྾‫ע‬؊൞̤̫ͥͅ‫ܥ‬ෝȂ̯ͣͅȂພࡔఘ͂਽৽྾‫ͅۼ͈͂ע‬ంह̳ͥਅș͈௖ࡽैဥ͈ٜྶͬ࿒ঐ̳ȃ଎ˍȁβͺ߿τΓίΗȜβͺ߿τΓίΗȜ͉Ȃ๱ુͅ௖൳଻͈̞ࣞဲଷ‫ا‬τΓίΗȜ͂‫ا଻ڰ‬τΓίΗȜ̥ͣࢹ଼̯ͦͥȃဲଷ‫ا‬τΓίΗȜ͉ ITIM ̱ͬٚ̀ဲଷ‫ا‬ΏΈ΢σͬഥో̳༷֚ͥȂ‫଻ڰ‬‫ا‬τΓίΗȜ͉ ITAM ̱ͬٚ̀‫ا଻ڰ‬ΏΈ΢σͬഥో̳ͥȃĳĳ଎ˎȁဲଷ‫ا଻ڰ͍̤͢ا‬τΓίΗȜͥ͢ͅ΍ͼΠι΄υ;ͼσΑۜஅळ༾͈෇ে‫ࢹܥ‬;ͼσΑ͉ȂMHC ͈อ࡛ͬ೩‫΅̵̯ئ‬ρȜ T ळ༾ͥ͢ͅ෇েͬ൪ͦȂ̯ͣͅȂNK ळ༾͈ဲଷ‫ا‬τΓίΗȜ͈ς΄ϋΡ̱͂̀ MHC အ໦ঊͬอ࡛̳̭ͥ͂ͤ͢ͅȂNK ळ༾ͥ͢ͅळ༾વ‫̥ͣ͜଻ٺ‬൪̞ͦ̀ͥ
Ȫऒ଎ȫȃ༷֚Ȃۜஅ೷ࢯ଻͈ζ;Α͈ NK ळ༾͉́Ȃ਽৽ MHC අ։എ̈́ဲଷ‫ا‬τΓίΗȜͬอ࡛̳̭ͥ͂ͤ͢ͅȂMHC ͈อ࡛̱̹;ͼ
σΑۜஅळ༾ͬ։ુळ༾̱͂̀෇ে̧́ͥȃ̯ͣͅȂ;ͼσΑ͈
MHC အ໦ঊͅచ̳ͥ‫ا଻ڰ‬τΓίΗȜͬอ࡛̳̭ͥ͂̽̀͢ͅȂ;ͼσΑۜஅळ༾ͬୟޭഎͅવ‫̳ͥٺ‬Ȫֲ଎ȫ(Arase et al. Science 2002)ȃ

ĩĳĪ ;ͼσΑ͈ळ༾ඤ૟ව‫͈ࢹܥ‬ٜྶȁષܱ͈̠͢ͅȂਅș͈;ͼσΑȂඅͅȂ঵௽ۜஅ̳ͬͥ;ͼσΑ͉Ȃဲଷ‫ا‬τΓίΗȜ͈ς΄ϋΡͬอ࡛̱྾‫ע‬؊൞ͬဲଷ̳ͥȃ‫ྙޟ‬૬̞̭͂ͅȂ;ͼσΑ͈ಎ͉ͅဲଷ‫ا‬τΓίΗȜ͈͂௖ࡽैဥͬဥ̞̀਽৽ळ༾͈૟ව͜ͅ

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‫۾‬ဓ̱̞̭̦̀ͥ͂ྶ̧̥̹ͣ̈́̽̀ͅȪ଎ˏȫȃඅͅȂ;ͼσΑ௰̥͈ٜͣଢ଼͉ٜ́ྶ̯̥̹ͦ̈́̽໦ঊι΃ΣΒθ̦྾‫ע‬τΓίΗȜ͂;ͼσΑ໦ঊ͈ٜ͂ଢ଼̽̀͢ͅ฻ྶ̱̹ȃ̷̭́Ȃ൚໦࿤͉́Ȃਅș͈ພࡔఘ͈਽৽ळ༾
͈͒૟ව‫̞̀̾ͅࢹܥ‬Ȃ਽৽໦ঊ̤͍͢;ͼσΑ໦ঊ஼༷͈௰࿂̥͈ٜͣྶͬ࿒ঐ̳ȃ଎ˏȁ;ͼσΑ͈ळ༾ඤ΀ϋΠςȜ͈໦ঊ‫͈ࢹܥ‬ٜྶ
;ͼσΑ͉ဲଷ‫ا‬τΓίΗȜ͈ς΄ϋΡͬอ࡛̳̭ͥ͂ͤ͢ͅ྾‫ע‬؊൞ͬဲଷ̳ͥȃِș͉Ȃဲଷ‫ا‬βͺ߿τΓίΗȜ͈֚̾ 3,/5Į̦ౙ੗ΰσβΑ;ͼσΑ +HUSHVVLPSOH[YLUXV ۜஅळ༾ͅอ࡛̳ͥ *O\FRSURWHLQ% ͬ෇ে̳̭ͥ͂ͬอࡉ̱̹ȃ̯ͣͅȂ*O\FRSURWHLQ% ͉ HSV ͈ۜஅͅຈଌ̈́΀ϋαυȜί໦ঊ̜̭̥́ͥ͂ͣȂ3,/5Į͈ HSV1 ۜஅ̤̫ͥͅ‫ܥ‬ෝٜͬଢ଼̳ͥ͂Ȃ3,/5Į͉ౙ੗ΰσβΑ;ͼσΑ͈ळ༾ඤ΀ϋΠςȜͅ‫۾‬ဓ̱̞̭̦̀ͥ͂฻ྶ̱̹ȃ̹͘Ȃକ൛ఝેឃ૮;ͼσΑ (Varicella-zoster virus) ̞̾̀͜ͅ *O\FRSURWHLQ% ̦βͺ߿τΓίΗȜ͈̜֚̾́ͥ 0\HOLQDVVRFLDWHGJO\FRSURWHLQ 0$*6LJOHF ࣣ̱͂ٛȂ;ͼσΑ͈΀ϋΠςȜͅ‫۾‬ဓ̱̞̭̀ͥ͂ͬྶ̥̱̹ͣͅȃ̭͈အͅȂβͺ߿τΓίΗȜ͉྾‫ע‬؊൞͈ଷࢄͅ૬̩‫۾‬ဓ̳̥ͥ͊ͤ́
̩̈́Ȃ;ͼσΑ͈ळ༾ඤ૟ව͜ͅ‫۾‬ဓ̱̞̭̦̀ͥ͂ྶ̥̹ͣ̈́̽ͅȃड߃͈యນഎ̈́ა໲
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neural tissues. Biochem Biophys Res Commun 2011 Feb 18; 405(3): 428-433.
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 :DQJ-)DQ46DWRK7$ULL-/DQLHU//6SHDU3*.DZDJXFKL<$UDVH+%LQGLQJRIKHUSHVVLPSOH[YLUXVJO\FRSURWHLQ% J% WR3,/5ĮGHSHQGVRQVSHFLILFVLDO\ODWHG2OLQNHGJO\FDQVRQJ%J Virol. 2009 Dec ; 83(24):13042-5.
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Lanier LL, Arase H. PILRĮLVDKHUSHVVLPSOH[YLUXVHQWU\FRUHFHSWRUWKDWDVVRFLDWHVZLWKJO\FRSURWHLQ%Cell.
2008 Mar 21; 132(6): 935-44
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UHFRJQLWLRQRIPRXVH&'E\SDLUHGLPPXQRJOREXOLQOLNHW\SHUHFHSWRU 3,/5 J Immunol. 2008 Feb 1; 180(3):1686-93ĳĴࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝໦ঊ֒ഥࡄ‫ݪ‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ġၑ‫ڠ‬ฎআȁ࿤ോȁฎ੎‫ޗ‬਎ġ
֓‫ڠ‬ฎআȁᩊനȁ‫֚ܮ‬੩‫ޗ‬Ȫ࠳ȫġ ֓‫ڠ‬ฎআȁ؈ॄȁఱٚඅහࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁඤ൥ȁုঊȁࡄ‫ݪ‬ඤယȇ൚ࡄ‫ݪ‬໦࿤͉́‫͈܈‬՛଻‫ฺ̠ͅا‬அ૗ఘະհ೰଻͈ଷࢄ‫ͬࢹܥ‬Ȃळ༾ਔ‫ܢ‬ΙͿΛ·εͼϋΠଷࢄ͞ಎ૤ఘ଼੃͈։ુ̞̠͂۷ത̥ͣࡄ‫̱̀ݪ‬̞ͥȃ̩͂ͅಎ૤ఘͅޫह̳ͥ Ser/Thr ΅΢ȜΔ̜́ͥ Lats1/2 ̜̞͉ͥ GAK ̦ࢹ଼̳ͥໝࣣఘ͈ȂDNA ੱ‫͈̥ͣޏ۪̠̞͂ٺ‬ΑΠτᾼచ̳ͥ؊൞
͈ଷࢄ‫ͅࢹܥ‬എͬࣆٜ̽̀ଢ଼̱̞̀ͥȃ̭ͦͣˎ̾
͈ΞȜζ͉ Mdm2 ͞ p53 ̱ͬٚ̀ࡽ̞ͅྟ୪ͅႲࠒ
̱̞̀ͥȪ଎ˍȫȃ଎ˍȈLats ΈσȜί͂ GAK ΈσȜί͉ࡽ̞ͅႲࠒ̱̞̀ͥȃȪˍȫLatsȪLarge tumor suppressorȫΈσȜί
Lats1, Lats2 ͉ਅ‫́ۼ‬༗ం̯̹ͦಎ૤ఘͅޫह̳ͥ႒য̈́΅΢ȜΔ̜́ͥȪToji et al., Genes Cells, 2004ȫȃLats1/2 ͉‫ۗܕ‬΍ͼΒͬଷࢄ̳ͥ Hippo pathway ̤̞̀ͅಎଥഎ̈́࿨‫༷̳̹֚́ضͬڬ‬Ȃळ༾ਔ‫ܢ‬ΙͿΛ·εͼϋΠଷࢄ̤̞̀͜ͅਹါ̈́‫ܥ‬ෝͬ౜̞̽̀
ͥȃ̩͂ͅ Lats2 ͉‫ဲ܈‬ଷ֦ঊ p53 ͈ೄ୪എ̈́ഢৢດഎ̜́ͥ͂
͂͜ͅ p53 ͈໦ٜͬဲଷ̳̭ͥ͂ͤ͢ͅ໦ႨȪMȫ‫޳̫̤ͥͅܢ‬൝̈́அ૗ఘ໦෻ͬଷࢄ̱̞̀ͥȃِș͉ո‫࡛͈ئ‬યͬࡉ੄̧̱̀
̹ȃ❶Lats2 ͈֒ഥঊࠧ৐ȪLats2 KOȫζ;Αͬैୋ̱ٜଢ଼̱̹ࠫ‫ض‬Ȃ႒য֒ഥঊ Lats1 ͉͂։̈́ͤ᥷͈อ୆Ȇ໦‫ͅا‬ຈଌ̈́֒ഥঊ̜́̽

଎ 2ȈLats2 ࠧఅͥ͢ͅಎ૤ఘ͈౯༌‫ا‬ȃ̹ȃ❷Lats2 KO ͈᥷ဇြ஌֋ٔळ༾‫ڼ‬ȪLats2-/- MEFȫ͉́ಎ૤ఘ͈౯༌‫ا‬Ȃஅ૗ఘ͈ະାႥȂळ༾ৗ໦Ⴈ͈։ુ̦۷ख़̯̹ͦȃ̭͈ͦͣࠫ‫͉ض‬ȂLats2 ̦ୃુ̈́ M ‫࣐ͅૺܢ‬ຈଌ̈́΅΢ȜΔ̜́ͥ
̭͂ͬাऐ̱̞̀ͥȪ଎ˎ ; Yabuta et al., J. Biol. Chem., 2007ȫȃ❸ِș͉ Lats2 ̦ଷࢄ̳ͥˎ͈̾૧̹̈́ςϋॸ‫ا‬ΏΈ΢σࠐႹȪCLP ࠐႹȂALB ࠐႹȫͬࡉ੄̱̹ȃCLP ࠐႹ̤̞̀ͅ Lats2 ͉ট‫ٸ‬஌ચৣͥ͢ͅ DNA అੱΙͿΛ·εͼϋΠͅ؊൞̱Ȃ14-3-3ཱུ̱ͬٚ̀࿫ဲଷͅ‫ ͥͩ۾‬P-body (processing body) ͈ࠁ଼ͬଷࢄ
̳ͥȪ଎ˏȈGW182 ͉ P-body ͈ࢹ଼֦ঊȫȃȁ༷֚Ȃ❹ALB ࠐႹ͉́ M ‫ ̞̤࣐̀ͅૺܢ‬Lats2 ͉ Aurora-A ΅΢Ȝ଎ 3ȈLats2 ͉ UV ચৣࢃ͈ P-body ͈ࠁ଼ͅຈါ̜́ͥȃΔͤ͢ͅςϋॸ‫̯̀ͦا‬அ૗ఘ̤͍͢ΓϋΠρσΑάϋΡσ
ȪCSȫષͅޫह̱ Lats1 ͂ Aurora-B ΅΢ȜΔͬଷࢄ̱̀ୃ‫̈́ږ‬அ૗ఘ໦෻ͬ৘࣐̳ͥȪ଎ːȫȃ̯ͣͅȂ
❺Lats2 ͉ ASPP1-p53 ͬଷࢄ̳̭ͥ͂ͤ͢ͅఉ෼ఘȆ։ତఘ͈՛଻‫܈‬ळ༾̤̞̀ͅळ༾ঘͬ
ည൵̱Ȫა໲ 5ȫȂSnail ͬςϋॸ‫ ̭̳ͤ͂ͥ͢ͅا‬EMTȪષ๧‫ۼ‬ဩഢ۟ȫͬଷࢄ̱̞̀ͥȪა໲ 2ȫȃ

଎ 4ȈLats2 ͉ Aurora-B ͂ CS ́‫ޫވ‬ह̳ͥȃȪˎȫGAKȪcyclin G-associated kinaseȫİcyclin G ΈσȜίȁȁȁِș̦อࡉ̱̹ GAK ͉ෞ૰ࠐळ༾ո‫͈ٸ‬ळ༾ৗ́·ρΑςϋ๭ໞ઀༾͈౎๭ໞͬଷࢄ྄̳̭ͥ͂́࿶௣Ȫ΀ϋΡ΍ͼΠȜΏΑȫͅຈଌ̜̦́ͥȂ̭͈࡛ય͉ͅ΅΢ȜΔႀ֖͉ະါ̜́ͥȃȁِș͉ո‫࡛͈ئ‬યͬࡉ੄̳̭͂́ GAK ΅΢ȜΔ͈࿨‫ͬڬ‬ٜྶ̧̱̹̀Ȫ଎ˑȫȃ❶GAK ͉ 33$%¶Ṳ͍̑͢ĳĵ

cyclin GȪcyclin G1Ȃcyclin G2ȫ͂ໝࣣఘͬࠁ଼̳ͥȃGAK ͉ 33$%¶Ȗ͈ T104 ͬςϋॸ‫ ̱ا‬PP2A ͈౎ςϋॸ‫ا‬ࣖள‫ͬ଻ڰ‬ଷࢄ̱̞̀ͥȪNaito et al., Cell Cycle, 2012; ଎˒ȫȃ❷GAK ͉ळ༾ৗ͈͙̩́̈́ M ‫̞̤̀ͅܢ‬ಎ૤ఘ͞‫ړ‬ඤ͜ͅޫह̱Ȃಎ૤ఘ଼੃͂அ૗ఘާੀȆାႥͬଷࢄ̱̞̀ͥȃ❸GAK ͬΦΛ·Θ;ϋ̳ͥ͂Ȃஅ૗ఘାႥ։ુ
̭̱ͬܳȂ̷ͦͬۜ౶̱̹ΑάϋΡσΙͿΛ·εͼϋΠ͈‫̀̽͢ͅا଻ڰ‬ळ༾ਔ‫ ̦ܢ‬M ‫́ܢ‬೪গ̳ͥȪ଎˓ȫȃ
❹
GAK ͈΅΢ȜΔ‫ࠧͬ଻ڰ‬అ̵̯̹ KO ζ;ΑȪGAK-kdȫͬैୋ̱ٜଢ଼̱̹̭͂ͧȂ෹͈อ୆ͅ։ુͬြ̱੄୆ೄ
ࢃͅଚ৻̱̀ঘཌͅঢ̹̽Ȫ଎˔ȫȃ໦ঊດഎ࿪͈ΊέͻΙΣήȪgefitinibȫ͉ GAK ͜ EGFR ͂൳೾ഽͅஷ‫̳ͥٺ‬̭̥͂ͣȂ̭͈ࠫ‫ ͉ض‬GAK ͈ஷ‫ۼ̦ٺ‬ৗ଻෹‫̠̞͂׶‬ਹඈ̈́໗ैဥͬ୆̲̞̭̀ͥ͂ͬাऐ̳ͥȃ

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଎ 5ȈGAK ͂ auxilin ͈ࢹ௮͉ GAK ͈΅΢ȜΔႀ֖ո‫͉ٸ‬႒য̱̞̀ͥȃ଎ 6ȈGAK ͥ͢ͅςϋॸ‫ا‬໐պ T104 ͉ %¶Ȗ͈σȜίˎͅպ౾̱Ȃ‫଻ڰ‬ଷࢄͅਹါ̜̭̦́ͥ͂াऐ̯ͦͥȃ଎ 7ȈGAK ͬ siRNA (Ki9) ́ΦΛ·Θ;ϋ̳ͥ͂அ૗ఘ
։ુ̭̳ͬܳȃ(Shimizu et al., J. Cell Sci., 2009)଎ 8ȈGAK ͈΅΢ȜΔࠧఅ͉ζ;Αథॾ‫͈ܢ‬෹‫ܥ‬ෝ଼͈੃ͅ։ુͬြ̳ȃȪა໲ˍͤ͢ȫड߃͈యນഎ̈́ა໲
1. Tabara H, Naito Y, Ito A, Katsuma A, Sakurai MA, Ohno S, Shimizu H, Yabuta N, Nojima H. Neonatal lethality inknockout mice expressing the kinase-dead form of the gefitinib target GAK is caused by pulmonary dysfunction.
PLoS ONE. 2011, 6(10): e26034.
2. Zhang K, Rodriguez-Aznar E, Yabuta N, Owen RJ, Mingot JM, Nojima H, Nieto MA, Longmore GD. Lats2 kinasepotentiates Snail1 activity by promoting nuclear retention upon phosphorylation. EMBO J. 2011 Sep 27;31(1):29-43.
3. Yabuta N, Mukai S, Okada N, Aylon Y, Nojima H. The tumor suppressor Lats2 is pivotal in Aurora A and Aurora B
signaling during mitosis. Cell Cycle. 2011, 10(16):2724-36.
4. Okada N, Yabuta N, Suzuki H, Aylon Y, Oren M, Nojima H. A novel Chk1/2-Lats2-14-3-3 signaling pathway
regulates P-body formation in response to UV damage. J. Cell Sci. 2011 Jan 1;124(Pt 1):57-67.
5. Aylon Y, Ofir-Rosenfeld, Y., Yabuta N, Lap, E. Nojima H, Lu, X. and Oren M. The Lats2 tumor suppressor
augments p53-mediated apoptosis by promoting the nuclear proapoptotic function of ASPP1. Genes Dev. 2010 Nov
1;24(21):2420-9.
ĳĶࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝อ‫܈‬ଷࢄࡄ‫ݪ‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁȁ‫ؖ‬നȁٗ૽੎‫ޗ‬਎ȁྴനȁ࿆෗੎‫ޗ‬਎ȁ઀आ५ȁ୷पඅහࡄ‫֥ݪ‬ġġ‫ࡔک‬ȁ࠲ఊ჊අහࡄ‫֥ݪ‬ġġ‫ࡓڙ‬ȁ‫ޕ‬ঊȁȶ̦ͭȷ͉ȂΊΦθͅ୆̲ͥအș̈́་։̧ͬ֨߄̱͂̀อ୆̱Ȃૺ‫̱ا‬Ȃ̷̱̀՛଻‫̳ͥا‬ȃ̷ً͈೾̤̞̀ͅȂȶ̦ͭဲଷ֒ഥঊȷ͈‫ܥ‬ෝࠧఅ་։ͥ͢ͅळ༾͈ະঘ‫͞ا‬Ȃȶ̦ͭࡔ֒ഥঊȷ͈‫ا଻ڰ‬་։Ȫȶ̦ͭ֒ഥঊȷ͈͒་։ȫͥ͢ͅळ༾ࠁৗ͈ఱ̧̈́ഢ̦۟୆̲ͥȃະঘ‫̦ͭ̀̽͢ͅا‬ཡࢄ‫͈̱̀͂ࢹܥ‬ͺεΠȜΏΑ͞Ⴧ‫̦ا‬ٝ๰̯ͦȂࠁৗഢ۟̽̀͢ͅুၙഎ௩૓ෝ͈‫ڕ‬ංȂळ༾‫ۼ‬΋ηνΣΉȜΏοϋ͈෫౦Ȃळ༾ࠁఠ͈་‫ا‬Ȃ‫ܖ‬ৗ໦ٜࣖள͞௩૓֦
ঊ͈໦๥ᄤૺȂ૫੕Ȇഢ֊ෝ͈‫ڕ‬ං͈̦̈́̓ͭ՛଻‫ࠁا‬ৗ̦ည൵̯ͦͥȃ൚ࡄ‫ݪ‬৒͉́Ȃࢃ৪͈ࠁৗഢ۟ͅ‫ͥͩ۾‬ȶ̦ͭࡔ֒ഥঊȷུ͈ြ͈୆ၑ‫ܥ‬ෝ͂ଷࢄ‫̴ͬ͘ࢹܥ‬ၑٜ̱Ȃ̷͈‫ا଻ڰ‬་։̦ͥͭ͢ͅࠁৗอ࡛͈໦ঊ‫ܖ‬๕͈ٜྶ͂
૧̹̦̈́ͭহၷດഎ͈‫ͬఽٳ‬࿒ঐ̱̹ࡄ‫̞ͥ̀͛ૺͬݪ‬ȃ̭ͦ́͘ͅȂΪΠ͈̦ͭ͂͜૬̩‫ͥͩ۾‬ΙυΏϋ΅΢ȜΔ߿̦ͭࡔ֒ഥঊ c-Src ͈อ୆Ȇ໦‫ا‬Ȇழ૕ࢹಃ̤̫̈́̓ͥͅ୆ၑ‫ܥ‬ෝȂ̷̤͍͈͢಺୯‫̧̹̱̥̀ͣͅྶͬࢹܥ‬ȃ࡛ह͉Ȃ̦ͭ‫ا‬κΟσळ༾͞৘ष͈ΪΠ̦ͭळ༾̈́̓ͬဥ̞̀Ȃc-Src ̦ͥͭ͢ͅ՛଻‫͈ا‬ळ༾ඤࠐႹ̷͈͂ଷࢄ
‫͈ࢹܥ‬஠ယٜྶͬ࿒ঐ̱̹ࡄ‫̞ͥ̀͛ૺͬݪ‬ȃ
I. c-Src ͈‫ܥ‬ෝ͂ଷࢄ‫ࢹܥ‬ȁୃુळ༾͉́Ȃc-Src ͉අ։എ̈́ଷࢄ֦ঊ Csk ̽̀͢ͅςϋॸ‫̹̯ͦا‬ະ‫́߿଻ڰ‬ంह̱Ȃ௩૓֦ঊ͞ळ༾‫ٸ‬ζΠςΛ·Α̈́̓ఉအ̈́ळ༾‫ࠣͅঁٸ‬؊൞̱̹শͅ‫̳ͥا଻ڰ‬ȃ̷͈‫ئ‬ၠ́ȂMAPK ࠐႹ͈̈́̓‫ͬا଻ڰ‬̱ٚ̀֒ഥঊ͈อ࡛ͬည൵̱̹ͤȂRho GTPase ͬ‫̱̀ا଻ڰ‬ळ༾ࣸ‫͈ڒ‬ठ༎ͬည൵̳ͥȃ̷͈ࠫ‫ض‬Ȃळ༾୪಍Ȇ‫׋‬൲ෝ͈‫ا଻ڰ‬Ȃ໦‫ا‬Ȃ௩૓Ȃ୆ంȂ̯͉ͣͅࠁৗഢ͈۟ည൵̈́̓ఉ
အ̈́୆ၑ‫ܥ‬ෝͬอ‫̳ͥܞ‬Ȫ଎ˍȫȃΪΠ͈̦͉ͭ́ c-Src ֒ഥঊুఘ͉ͅ་։͉࠿੄̯̞̞̦ͦ̀̈́Ȃအș̦̈́ͭळ༾́
Src ͈౩ฒၾ͞‫̦଻ڰ‬ષઌ̱̞̭̦̀ͥ͂෇̞͛ͣͦ̀ͥȃ̷͈௩ఱ̱̹΅΢ȜΔ‫ ̀̽͢ͅ଻ڰ‬Src ͈ఉအ̈́‫ܥ‬ෝ̦௩໙̯̦ͦ̀ͭ՛଻‫ࠁا‬ৗ̦ည൵̯ͦͥ͂ࣉ̢̞ͣͦ̀ͥȃ߃ාِș͉Ȃc-Src ͈̦ͭ‫ا‬ෝ̦Ȃळ༾྄η·υΡιͼϋͅంह྄̳ͥͺΘίΗȜΗϋΩ·ৗ Cbp ̽̀͢ͅଷࢄ̯̭ͦͥ͂ͬࡉ
੄̱Ȃ̷͈ଷࢄΏΑΞθ͈෫౦̦͂ͭ‫۾͈͂ا‬Ⴒ଻̞̾̀ͅ
ٜଢ଼ͬૺ̞͛̀ͥȃII. c-Src ̦ͥͭ͢ͅࠁৗอ࡛‫͈ࢹܥ‬ٜଢ଼଎ˍȅc-Src ͈‫ܥ‬ෝ͂‫଻ڰ‬ଷࢄ‫ࢹܥ‬

ȁc-Src ̦ͥͭ͢ͅࠁৗอ࡛ͅঢͥळ༾ඤࠐႹͬඅ೰̳̹ͥ͛
ͅȂඊুͅ‫ٳ‬อ̱̹ c-Src ̦ͥͭ͢ͅ‫ا‬ည൵ࠏͬဥ̞̀࿌္
എٜ̈́ଢ଼ͬૺ̞͛̀ͥȃίυΞ΂η·Αٜଢ଼̥ͣȂ૧̹̈́
Src ‫ئ‬ၠ֦ঊ̱͂̀ Rho ͈‫ͬا଻ڰ‬ည൵̳ͥ Arhgef5 ͬ൳೰̱Ȃ̷͈ KO ζ;Αैୋ൝ͤ͢ͅ‫ܥ‬ෝٜଢ଼ͬૺ̞͛̀ͥȃ̹͘Ȃ
miRNA ͈ͺτͼٜଢ଼̥͉ͣȂc-Src ̦ͥͭ͢ͅ‫ࠁا‬ৗ͈อฺ࡛̽̀ͅ๤‫ڛ‬എࡠ̹ͣͦਅ႒͈ miRNA ͈อ࡛̦་൲̳̭ͥ
͂ͬࡉ̞̺̱Ȃ̷͈ͦͣດഎ֒ഥঊͬໝତਅ൳೰̳̭ͥ͂ͅ
଼̱̞ࢗ̀ͥȃ̷ͦͣ৽ါ̈́ດഎ֒ഥঊ̦ c-Src ͈ષၠ̤͢
͍‫ئ‬ၠ͈ΏΈ΢σ໦ঊȪFGFR3, mTOR, ILK ̈́̓ȫ̜̭̥́ͥ͂ͣȂc-Src ̦ miRNA ̱ͬٚ̀ু͈ͣΏΈ΢σഥోࠐႹͬ
ΙνȜΣϋΈ̳̭ͥ͂̽̀͢ͅȂ̦ͭ‫ࠁا‬ৗ͈อ࡛ͬεΐĳķ଎ˎȅmiRNA ̳ͬٚͥ Src ̦ͥͭ͢ͅࠁৗอ࡛ଷࢄ‫ࢹܥ‬Ξͻῄ΋ϋΠυȜσ̳̞̠ͥ͂૧̱̞ଷࢄΏΑΞθ͈ంहͬྶ̥̱̹ͣͅȪ଎ˎȫȃ
III. ࢃ‫΀ܢ‬ϋΡΕȜθȟςΕΕȜθ̳ͬٚͥ௩૓ΏΈ΢σ͈ٜଢ଼

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ȁc-Src ͈ດഎ໦ঊౝً͈॑೾́Ȃࢃ‫΀ܢ‬ϋΡΕȜθȟςΕΕȜθͅඅ։എͅޫह̳ͥ૧྄̹̈́ͺΘίΗȜ౩ฒৗ
p18 ͬ൳೰̱Ȃ̷͈‫ܥ‬ෝٜଢ଼ͬૺ̞͛̀ͥȃ̭ͦ́͘ͅȂ
p18 ̦ p14/MP1 ͂ ໝ ࣣ ఘ ͬ ࠁ ଼ ̳ ͥ ̭ ͂ ͅ ͢ ̽ ̀ȂmTORC1 ̦ࢃ‫΀ܢ‬ϋΡΕȜθȟςΕΕȜθષ́‫̳ͥا଻ڰ‬̹͛ͅຈଌ͂̈́ͥ௷ા̱͂̀‫ܥ‬ෝ̳̭ͥ͂ͬྶ̥̱ͣͅ
̹ȃ̹͘Ȃp18 KO ζ;Α̦ςΕΕȜθ଼͈੃ͅਹඈ̈́։
ુͬা̱̀థ୆౿ঘ̭̥͂̈́ͥ͂ͣȂςΕΕȜθ଼੃ً೾ͅ ̤ ̫ ͥ p18 ͈ ࿨ ‫ ̦ ڬ‬ಕ ࿒ ̯ ͦȂ̷ ͈ ष ͅ ‫ ܥ‬ෝ ̳ ͥ
p18-mTORC1 ࠐႹ͈ດഎ໦ঊ͈൳೰͂‫ܥ‬ෝٜଢ଼ͬૺ̞͛̀
p18-mTORC1 ࠐႹ̦Ȃळ༾͈௩૓଼͞ಿȂͥȪ଎ˏȫȃ̯ͣͅȂ‫ ͍ݞ‬Src ͈̦̈́̓ͭ֒ഥঊ̳̦ͬٚͥͭ‫̞̤̀͜ͅا‬ຈଌ͈࿨‫ͬڬ‬౜̠̭̥͂ͣȂ̷͈௩૓ΏΈ΢σࠐႹ̤̫ͥͅ‫ܥ‬଎ˏȅ૧̹̈́ࢃ‫΀ܢ‬ϋΡΕȜθ྄ͺΘίΗȜ౩ฒৗ p18 ͈‫ܥ‬ෝෝ̞̾̀͜ͅ໦ঊτασ͈ٜ́ଢ଼ͬૺ̞͛̀ͥȃड߃͈యນഎ̈́ა໲
1 : Takahashi Y, Nada S, Mori S, Soma-Nagae T, Oneyama C, Okada M. The late endosome/lysosome-anchoredp18-mTORC1 pathway controls terminal maturation of lysosomes. Biochem Biophys Res Commun. 2012 Jan
27;417(4):1151-7.
2 : Oneyama C, Morii E, Okuzaki D, Takahashi Y, Ikeda J, Wakabayashi N, Akamatsu H, Tsujimoto M, Nishida T,
Aozasa K, Okada M. MicroRNA-mediated upregulation of integrin-linked kinase promotes Src-induced tumor
progression. Oncogene. 2012 Mar 29;31(13):1623-35.
3 : Kuroiwa M, Oneyama C, Nada S, Okada M. The guanine nucleotide exchange factor Arhgef5 plays crucial roles in
Src-induced podosome formation. J Cell Sci. 2011 May 15;124(Pt 10):1726-38.
4 : Oneyama C, Ikeda J, Okuzaki D, Suzuki K, Kanou T, Shintani Y, Morii E, Okumura M, Aozasa K, Okada M.
MicroRNA-mediated downregulation of mTOR/FGFR3 controls tumor growth induced by Src-related oncogenic
pathways. Oncogene. 2011 Aug 11;30(32):3489-501.
5 : Oneyama C, Hikita T, Enya K, Dobenecker MW, Saito K, Nada S, Tarakhovsky A, Okada M. The lipid raft-anchoredadaptor protein Cbp controls the oncogenic potential of c-Src. Mol Cell. 2008 May 23;30(4):426-36.
ĳĸࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝ૂ༭ഥో໦࿤
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁࣞாȁ૝ࢨ੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁ࿐࡫‫ؚ‬ȁࢼࢫ੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁඤ൥ȁંൽඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁ‫܀‬ġġġ‫י‬璐අහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁ५୼ȁఱঃεΑΡ·ȁȁ֓‫ڠ‬ฎআȁ֏‫ڦ‬ȁဇ๼εΑΡ·ȁȁ࿪‫ڠ‬ฎআȁ࿉၌ȁ࿻๼ࡄ‫ݪ‬ඤယȇୃુழ૕Ȇ௫‫ܕ‬ȟ‫ࢹ͈ۗܕ‬ಃ̤̞͉̀ͅȂழ૕අ։എۚळ༾ͥ͢ͅழ૕ळ༾͈ॲ୆̦ຈଌ̜̦́ͥȂ൳শ̭͈ۚͅळ༾ͬಎ૤̱̹͂Ȃழ૕۪‫ࢹ͈ޏ‬ಃ̦̭ͦͣۚळ༾ΏΑΞθͬ֋঵̱̞̩̀ષ͉́ਹါ́
̜ͥȃழ૕۪‫͈ޏ‬ါள̱͂̀Ȃ‫̜ͤ́ۯ̦͈̳ࠬ̈́ͬڒུࣸܖ‬Ȃࠬ‫ࢹۯ‬ಃ̦̫͕͈̈́ͦ͊͂ͭ̓௫‫ܕ‬Ȇ‫଼͉ࠁ͈ۗܕ‬ஷ‫̯ͥͦٺ‬ȃِș͈ࡄ‫ݪ‬৒͉́Ȃ̭͈̠̈́͢ୃુ௫‫ܕ‬Ȇ‫ۯ̫̤ࠬͥۗͅܕ‬૧୆͂ழ૕ۚळ
༾͈ۚळ༾଻͈֋঵‫͈̞̀̾ͅࢹܥ‬໦ঊ‫ͬࢹܥ‬ٜྶ̱Ȃඅͅພఠࠁ଼͈͂‫͉̞̤̀ͤͩͅ۾‬ਆនͅಕ࿒
̱̀Ȃ̦ͭۚळ༾͈อ୆ȟ௩૓ȟ֋঵͈ι΃ΣΒθ͂Ȃ̷ͦͬ঑঵̳ͥ୆ఠ‫ڠ‬എഐਫ਼ȪΣΛΙȫٜͬଢ଼̱Ȃ̦ͭͬआহ̳ͥহၷ༹͈‫ٳ‬อ࣐̞ͬ̽̀ͥȃࡄ‫͉ݪ‬ఱ̧̩໦̫ո‫ࣜˎ͈ئ‬࿒ͤ͢ͅ৘ঔ̱̞̀ͥȃԅ. ୃુழ૕̤͍̦ͭ͢ழ૕̤̫ͥࠬͅ‫ۯ‬ςκΟςϋΈ͈໦ঊ‫ܥ‬੬͈ٜྶȁˍȫȁอٔഎࠬ‫ۯ‬૧୆͈໦ঊι΃ΣΒθȁ(Tie1, Tie2 ਋ယఘ͈‫ا଻ڰ‬Ȃະ‫͈ا଻ڰ‬໦ঊ‫) ࢹܥ‬ȁˎȫȁ଼ఘࠬ‫ۚۯ‬ळ༾͈อ୆͂୆ၑഎȟພഎࠬ‫۾଼͈͂ࠁۯ‬Ⴒȁˏȫȁࠬ‫͍̤͢ا੃଼ۯ‬൲୓ྤΩΗȜΣϋΈ͈ٜྶȪephrinB2/EphB4Ȃapelin/APJȫȁːȫȁࠬ‫ۯ‬අ։എ̈́ΡρΛΈΟςΨςȜΏΑΞθ͈ࢹಃ
Ԇ. ̦ͭۚळ༾̤͍͢ୃુழ૕ۚळ༾͈ழ૕ඤ֋঵‫͈ࢹܥ‬ٜྶȁˍȫȁۚळ༾͈͒ςίυΈρηϋΈ‫͈ࢹܥ‬ٜଢ଼ȁˎȫȁۚळ༾͈໦Ⴈͬଷࢄ̳ͥ DNA ໝୋ֦ঊ GINS ̤͍͢ Galectin-3 ͈‫ܥ‬ෝଷࢄȁˏȫȁ̦ͭۚळ༾֋঵̥̥ͩͥͅΣΛΙࢹ଼ळ༾͈൳೰͂ΣΛΙ͈෫౦଎ˍȁࠬ‫ۯ‬૧୆͂ࠬ‫ۯ‬ඤ๧ۚळ༾
ࠬ‫ۯ‬૧୆ً͈೾͉́Ȃอ̱̹ٔࠬ‫୶͈ۯ‬൮ͬ֊൲̱̀΄ͼΘϋΑ̱͂̀‫ܥ‬ෝ̳ͥ tip ळ༾Ȃ̷͈෸ࢃ̥ͣ௩૓
̱̀ࠬ‫ۯ‬૝ಿͅ‫ ͥͩ۾‬stalk ळ༾Ȃ̷̱̀डࢃͅ༃ळ༾‫ͅۯ̹̱ࠬ੃଼ฺ̠ͬا‬໦‫ ̵̯ͥا‬phalanx ळ༾̞̠͂Ȃઁ̩̈́͂͜ˏਅ͈ඤ๧ळ༾̦ంह̳̭̦ͥ͂ྶ̧̥̯̹ͣͦ̀ͅȃِș͉ඤ๧ळ༾͈ಎۚͅळ༾အळ༾
ȪspECȫͬอࡉ̱̹̭͂ͅၛ‫̱ݗ‬Ȃ̭͈̠̈́͢ඤ๧ळ༾͈։ਅ଻ळ༾໦‫̫̤ۚͥͅا‬ळ༾ΏΑΞθ͈୆ၑഎ̤
͍͢ພഎփٜ݅ͬྶ̱̞̀ͥȃĳĹ଎ˎȁ̦ͭۚळ༾͈ࠬ‫ۯ‬ΣΛΙِș͉Ȃুࡨໝୋಎ͈ۚळ༾ͅ‫ވ‬೒̱̀อ࡛̳ͥ DNA ໝୋ֦ঊȂPSF1 ͈อ࡛ͬ၌ဥ̱̦̀ͭۚळ༾ȪCSCȫͬ௴̢Ȃ
CSC ̦ਆនඤ଼੃ࠬ‫ͬۯ‬୆ఠ‫ڠ‬എഐਫ਼ȪΣΛΙȫ̱̞̭͂̀ͥ͂ͬࡉ̞̺̱̹ȃࣽࢃȂࠬ‫ۯ‬ΣΛΙ̤̫ͥͅȂ̦ͭۚळ༾͈ۚळ༾଻֋঵‫͈ࢹܥ‬ٜྶ̦ਹါ̜́ͥȃ

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଎ˏȁࠬ‫ࢹܥا੃଼͈ۯ‬ਅș͈ࠬ‫ۯ‬ພ͈হၷ̤̞̀ࠬͅ‫ͼ͈ا੃଼ۯ‬ϋΩ·Π͉ఱ̧̞ȃ௮ࠬۚळ༾͞༃
ळ༾̦໦๥̳ͥ angiopoietin-1ȪAng1ȫ͉ඤ๧ळ༾ષ͈ Tie2 ͈‫ ́ا଻ڰ‬apelin ͈໦๥ͬ௯ૺ̵̯Ȃඤ๧ळ༾ͅอ࡛̳ͥ˓྄ٝ۹೒߿͈ GPCR ̜́ͥ apelin ͈਋ယఘAPJ ͬ‫̱̀ا଻ڰ‬Ȃඤ๧ळ༾͈௩૓͂͂͜ͅȂඤ๧ळ༾͈ਬࣣͬည൵̱̀Ȃࠬ‫ۯ‬ࠂͬ‫ڐ‬ఱ̵̯ͥȃVEGF/VEGFR2 ΏΑΞθ́ࠁ଼̯ͦͥࠬ‫͉͂ۯ‬։̈́ͤȂࠬ‫ۯ‬൫ً଻͜ଷࢄ̯̤ͦ̀ͤȂࠬၠ͈௩ఱ̦ພఠ٨஝ͅίρΑ͂̈́ͥহၷͅ apelin ͉ခ̜࢘́ͥȃड߃͈యນഎ̈́ა໲
1. Naito H, Kidoya H, Sakimoto S, Wakabayashi T, Takakura N. Identification and characterization of a resident
vascular stem/progenitor cell population in preexisting blood vessels. EMBO J. 2011 Dec 16;31(4):842-55.
2. Kidoya H, Naito H, Takakura N. Apelin induces enlarged and non-leaky blood vessels for functional recovery fromischemia. Blood. 2010 Apr 15;115(15):3166-74.
3. Nagahama Y, Ueno M, Miyamoto S, Morii E, Minami T, Mochizuki N, Saya H, Takakura N. (2010). PSF1, a DNA
replication factor expressed widely in stem and progenitor cells, drives tumorigenic and metastatic properties.
Cancer Res. 2010 Feb 1;70(3):1215-24.
4. Ueno M, Itoh M, Sugihara K, Asano M, and Takakura N. (2009). Both Alleles of PSF1 are Required for
Maintenance of Pool Size of Immature Hematopoietic Cells and Acute Bone Marrow Regeneration. Blood. 2009 Jan
15;113(3):555-62.
5. Kidoya H, Ueno M, Yamada Y, Mochizuki N, Nakata M, Yano T, Fujii R, Takakura N. (2008). Spatial and temporalrole of the apelin/APJ system in the caliber size regulation of blood vessels during angiogenesis. EMBO J. 2008 Feb
6;27(3):522-34.
ĳĺࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities۪‫ޏ‬؊൞ࡄ‫ݪ‬໐࿝ळ༾ଷࢄ໦࿤
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁฎআȪၑ‫ڠ‬ȫȁ२࿐ȁဉྶ੩‫ޗ‬ȁฎআȪ֓‫ڠ‬ȫȁ५ॄȁఱ༟੩‫ޗ‬ȁฎআȪ࿪‫ڠ‬ȫȁ஑࡫ȁဢ࿸අහࡄ‫֥ݪ‬ȁฎআȪ୆ྵ‫ڠش‬ȫġ ષ଩ȁ‫ح‬ජ๼අහࡄ‫֥ݪ‬ȁฎআȪ࿪‫ڠ‬ȫġ໹നȁဈٚȁఉळ༾୆໤ͬࢹ଼̳͈֚֚ͥ̾̾ळ༾͉Ȃ౾̥̹۪ͦ‫ޏ‬ȪγσκϋȆ௩૓֦ঊ͈̈́̓ঁࠣ໤ৗȂਔս͈ळ༾͞‫ۼ‬ৗ͈͂௖ࡽैဥ̈́̓ȫͅഐ୨ͅ؊൞̳̹͈ͥ͛ΏΈ΢σഥోΏΑΞθͬခ̱̞̀ͥȃ̷͈෫౦͉Ȃ̦ͭळ༾ͅങ߿
എͅ۷ख़̯̠ͦͥ̈́͢ਔս͈͂಺გͬ֝౎̱̹ྫಉ੬̈́ळ༾௩૓̈́̓Ȃ̯̰̈́͘͘৖͈ۛࡔ֦͂̈́ͥȃজ̹̻͈ࡄ
‫ݪ‬৒͉́Ȃळ༾͈௩૓Ȃ໦‫ا‬Ȃ‫׋‬൲̈́̓Ȃ̯̰̈́͘͘ळ༾࡛યͬଷࢄ̳ͥΏΈ΢σഥో͈ॽழ͙̞̾̀ͅȂ໦ঊȆळ༾τασ̥ͣ஌ಖ͞ζ;Αͬဥ̞̹୆໤ࡢఘτασ́༫‫گ‬എٜ̈́ଢ଼ͬૺ̞͛̀ͥȃඅ࡛ͅह͉Ȃ(1) ౩ฒৗ͈‫خ‬‫ݙ‬എ̈́ॸ‫ا‬ਘૐͥ͢ͅॸ‫ا‬ΑΠτΑ؊൞ΏΈ΢σഥోȂ(2) τΡΛ·Α؊൞֦ঊ̦ͥͭ͢ͅഢ֊͈௯ૺ̤͍͢ळ༾
ඤζΈΥΏ;θଷࢄȂ͈ඵതͬ৽ါ̈́ࡄ‫هݪ‬ఴ̱͂̀৾ͤழ̞ͭ́ͥȃȪˍȫ౩ฒৗ͈‫ݙخ‬എ̈́ॸ‫ا‬ਘૐͥ͢ͅॸ‫ا‬ΑΠτΑ؊൞ΏΈ΢σഥోȁఉळ༾୆໤͈੝‫ܢ‬อ୆͞อ̦ͭ̈́̓ͅਹါ̈́ Wnt ΏΈ΢σഥో͈૧ܰଷࢄ֦ঊ̱͂̀ȂΙ΂τΡ΅Ώϋ႒‫׻‬౩ฒৗ͈Τ·τ΂τΡ΅ΏϋȪNRX: nucleoredoxinȫͬࡉ̫̹̾ȃNRX ͉ Wnt ΏΈ΢σഥోͅຈଌ͈ DishevelledȪDvlȫࣣ̱ࠫ̀ͅȂ̷͈‫ܥ‬ෝͬဲଷ̳ͥ൱̧ͬ̾͜ȃ̷͈༷֚́Ȃ̭͈ NRX-Dvl ͈ࣣ͉ࠫȂNRX ͈໦ঊඤ S-S ࣣ͈ࠫࠁ଼ͥ͢ͅ‫ݙخ‬എ̈́ॸ‫̀̽͢ͅا‬ଷࢄ̯̞̭ͦ̀ͥ͂͜ྶ̥̹ͣ͂̈́̽ȃ̾ͤ͘ȂNRX ͉τΡΛ·Αેఠ؊൞଻ͅ Wnt ΏΈ΢σഥోͬ಺୯̳ͥ‫ܥ‬ෝͬ౜̞̽̀ͥȪFig. 1ȫȃ̹͘ȂΙ΂τΡ΅Ώϋͬ၌ဥ̱̀Ȃळ༾ඤ́ S-Sࣣࠫͬࠁ଼̳ͥ౩ฒৗͬ࿌္എͅౝ̳॑ͥ৘ࡑࠏͬ‫ٳ‬อ̱̹ȃ̷ͦͬဥ̞̀Ȃ૧ܰ S-S ౩ฒৗ̱͂̀Γζέ΁ςϋ
ΏΈ΢σഥోͅ൱̩ CRMP2 ̈́̓ͬࡉ̫̞̾̀ͥȃΓζέ΁ςϋঁࠣ؊൞଻ًͅॸ‫ا‬କள̦ॲ୆̯ͦȂ̷̦ͦ
CRMP2 ͬॸ‫ ̱̀ا‬S-S ࣣ̹ࠫ́ࠫ͊ͦγκඵၾఘͬै̵̭ͣͥ͂ͬྶ̥̱̹ͣͅȃ̭͈‫ݙخ‬എ̈́ CRMP2 ॸ‫̦ا‬Γζέ΁ςϋঁࠣͥ͢ͅ৊॑฽อ؊൞ͬಏ̱̞̭ٚ̀ͥ͂ͬอࡉ̱̹ȪFig. 2ȫȃ

Fig. 1: Redox regulation of Wnt signaling by reversible oxidation of NRX(A) Developmental abnormality of bones in NRX-/- mice. (B) Schematic illustration of the mechanism of redox regulation of Wnt signalingE\15;15;LQKLELWVWKHIXQFWLRQRI'YO7KHSURWHLQFRPSOH[FRPSRVHGRI$3&$[LQDQG*6.LQGXFHVGHJUDGDWLRQRIȕFDWHQLQ:KHQ15;LVR[LGL]HG'YOEHFRPHVIUHHIURP15;DQGLQKLELWVWKHGHJUDGDWLRQRIȕFDWHQLQZKLFKLQGXFHVWKHH[SUHVVLRQRILWVWDUJHW
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Fig. 2: CRMP2 oxidation in Semaphorin signaling(A) Repulsive guidance of axons by Semaphorin 3A (Sema3A). (B) Increase of H2 O 2 in growth cones of neurons by Sema3A. (C)Schematic illustration of the mechanism of Semaphorin signaling via CRMP2 oxidation. Sema3A stimulation generates H 2O2 and oxidizes
CRMP2. Oxidized CRMP2 transiently forms protein complexes with TRX, which induces CRMP2 phosphorylation by GSK3.

ȪˎȫτΡΛ·Α؊൞֦ঊ̦ͥͭ͢ͅഢ֊͈௯ૺ̤͍͢ळ༾ඤζΈΥΏ;θଷࢄȁS-S ࣣࠫͬैͥ౩ฒৗ͈ౝ̥॑ͣȂΙυΏϋγΑέ͹ΗȜΔΡιͼϋͬ঵̾‫ܥ‬ෝྚ౶͈౩ฒৗ PRL ͬࡉ̫̹̾ȃ
PRL ͉ഢ֊଻ఱಸ̦ͭ̈́̓՛଻ഽ͈̞̦͈ࣞͭ́ࣞอ࡛̦෇̤͛ͣͦ̀ͤȂ̦ͭഢ֊ͬ௯̳֦ঊ̱͂̀౶ͣͦ̀
̞ͥȃজ̹̻͉ PRL ͈ࣣࠫ౩ฒৗ͈ౝ࣐̞॑ͬȂMagnesium-Exporting proteinȪMagExȫ͂ࡤ྄͐౩ฒৗͬ൳೰̱̹ȃ
MagEx ͉ळ༾ඤ͈ Mg2+ ͬෳ੄̳̭ͥ͂́ळ༾ඤ Mg2+ ͈ଷࢄ࣐̤ͬ̽̀ͤȂPRL ̷͈ࣣ͈͂ࠫ̽̀͢ͅ‫ܥ‬ෝ̦ဲଷ̯̭ͦͥ͂ͬࡉ̫̞̾̀ͥȃड߃͈యນഎ̈́ა໲
1. Morinaka A, Funato Y, Uesugi K, Miki H. Oligomeric peroxiredoxin-I is an essential intermediate for p53 toactivate MST1 kinase and apoptosis. Oncogene. 2011 Oct 6;30(40):4208-18.
2. Morinaka A, Yamada M, Itofusa R, Funato Y, Yoshimura Y, Nakamura F, Yoshimura T, Kaibuchi K, Goshima Y,
Hoshino M, Kamiguchi H, Miki H. Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 andgrowth cone collapse. Sci Signal. 2011 Apr 26;4(170):ra26.
3. Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H. Nucleoredoxin sustains:QWȕFDWHQLQVLJQDOLQJE\UHWDLQLQJDSRRORILQDFWLYHGLVKHYHOOHGSURWHLQCurr Biol. 2010 Nov 9;20(21):1945-52.
4. Hayashi T, Funato Y, Terabayashi T, Morinaka A, Sakamoto R, Ichise H, Fukuda H, Yoshida N, Miki H.
Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of flightless-I to myeloid
differentiation primary response gene (88). J Biol Chem. 2010 Jun 11;285(24):18586-93.
5. Yoshimura Y, Terabayashi T, Miki H. Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B toregulate axon formation. Mol Cell Biol. 2010 May;30(9):2206-19.
ĴĲࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜ‫ޗ‬਎ȁȁȁȁၑ‫ڠ‬ฎআġཱ֔ȁੇࢤઉ̞͒‫ޗ‬਎ȁၑ‫ڠ‬ฎআȆ֓‫ڠ‬ฎআġ നᰦȁგ᫜੩‫ޗ‬ȁȁȁȁฎআ ĩ ၑ‫ ڠ‬Īġခྎȁ૝ঊ੩‫ޗ‬ȁȁȁȁฎআ ĩ ֓‫ ڠ‬Īȁġ൐‫܅‬ȁහࢬ

අහ੩‫ޗ‬ȁȁฎআ ĩ ࢥ‫ ڠ‬ĪȁȁΣςͺϋȆζςȜȆΩρΛ·ΩΛ·ġŒඅහࡄ‫֥ݪ‬ȁฎআ ĩ ၑ‫ ڠ‬Īȁส࿐ȁୃങ໦ঊࡔಖ‫ڠ‬໦࿤ࡄ‫ݪ‬ΈσȜίȁଲ‫͈࢛૽ٮ‬࿩ 4 ‫̦ڬ‬ζρςͺၠ࣐౷֖ͅ‫ݳ‬ਯ̱Ȃා‫ ̷̤͢ͅۼ‬200 ྔ૽̦ঘཌ̳̹ͥ͛Ȃζρςͺ͉૽႒डఱ͈
ഌ͂ࡤ̞͊ͦ̀ͥȃ̹͘Ȃၠ࣐౷֖͈஠֖̤̞̀ͅȂ࿪सఛ଻ζρςͺ̦੄࡛̱̤̀ͤȂζρςͺచॐ̦ࣾඳ̈́ͅ
̜ͤ̾̾ͥȃਲ̽̀Ȃχ·Ιϋ͞ࢯζρςͺ࿪स͈‫ٳ‬อ͉޽‫هݪࡄ͈ݢ‬ఴ̜́ͥȃ൚ࡄ‫ݪ‬໦࿤͉́ζρςͺχ·Ιϋ͈‫ٳ‬อ࣐̠ͬ͂͂͜ͅȂ໦ঊळ༾୆໤‫ڠ‬എ਀༹ͬဥ̞̀Ȃζρςͺࡔಖ͈‫ܙ‬୆ഐ؊୽ၞ͈ٜଢ଼࣐̞ͬ̽̀ͥȃȪˍȫழ̢۟ SERA ౩ฒৗͥ͢ͅζρςͺχ·Ιϋ͈‫ٳ‬อȁ໦ঊࡔಖ‫ڠ‬໦࿤͉́෎ఝ෎ζρςͺࡔಖ͈ SERA ౩ฒৗ͈֚໐ͬఱಸ޿́อ̵࡛̯̹ழ̢۟౩ฒৗȂSE36 ͬဥ̞̹ζρςͺχ·Ιϋ͈৘ဥ‫ͬݪࡄا‬ଔૺ̱̞̀ͥȃၠ࣐౷֖͈ࡄ‫ݪ‬৪͈͂‫ވ‬൳ࡄ‫̥ͣݪ‬Ȃζρςͺ͈ࣞഽၠ࣐౷
֖ਯྦྷ̤̞̀ͅুடͅ‫ڕ‬ං̯ͦͥζρςͺ྾‫̱ࠬ̀͂৽͉ע‬ୄಎͅంह̳ͥࢯ SERA IgG3 ࢯఘ̜̭́ͥ͂ͬྶͣ
̥̱̹ͅȃ̹͘ȂΙϋΩϋΐȜͬ‫͚܄‬ఉ̩͈൲໤ͬ SE36 ́྾‫̳͂ͥע‬Ȃζρςͺࡔಖ͈௩૓ͬဲଷ̳ͥࢯఘ̦ည
൵̯̞̠ͦͥ͂ࠫ‫ͬض‬ං̞̀ͥȃ࡛हȂ( ़ ) िఱ๷୆໤ພࡄ‫ݪ‬ٛ͂‫ވ‬൳̱̀ SE36 দैζρςͺχ·Ιϋ͈ၾॲఘଷͬಃ̞̹ȃ໹଼ 17 ා࣭ͤ͢ඤ̤̞̀ͅ SE36 ζρςͺχ·Ιϋ͈ల I ௖ႉ઄দࡑͬ৘ঔ̱Ȃ̷͈հ஠଻͂྾‫ע‬ࡔ଻̦‫̹̯ͦ෇ږ‬ȃχ·Ιϋ୪ਅͬঔ̱̹δρϋΞͻͺ͈૽͉́ 100% ͈ࢯఘုഢၚͬা̱̹ȃ࡛ह͉ͺές΃͈;΄ϋΘ̤̞̀ͅల I ௖ b ႉ઄দࡑͬ৘ঔಎ̜́ͥȃུίυΐͿ·Π͉ ( ़ ) िఱ๷୆໤ພࡄ‫ݪ‬͈ٛ͂‫ވ‬൳‫ٳ‬อίυΐͿ·Π̱͂̀৘ঔ̱̞̀ͥȃȁ෎ఝ෎ζρςͺࡔಖ͈ SERA ౩ฒৗ͈‫ܥ‬ෝٜଢ଼͞อ࡛ٜଢ଼Ȃ̯ͣͅ SERA ͅచ̳ͥ਽৽͈྾‫ע‬؊൞͈̈́̓‫ܖ‬யࡄ‫͜ͅݪ‬ୈႁͬಕ̞̞́ͥȃ̹͘Ȃ२඾෎ζρςͺ͈χ·Ιϋ‫ٳ‬อ͜ͅ಍਀̱̤̀ͤȂૺ‫ࠏا‬ൡഎٜ̈́ଢ଼̥ͣχ·Ιϋ࢓༞ࢯࡔͬౝ̳॑ͥ͂͂͜ͅȂ;΄ϋΘȂΗͼȂͼϋΡΥΏͺȂΕυκϋ੨ോ͈̈́̓ၠ࣐౷֖͂͜‫ވ‬൳ࡄ‫ͬݪ‬࣐̞Ȃۛ৪΍ϋίσ͈ٜଢ଼࣐̞̽̀ͥ͜ȃ଎ˍȇ;΄ϋΘཤ໐ͺΩΛΙ̜ͥͅພ͈֭
ఞࣣ৒ȇਹછζρςͺ͈ঊ‫ͬރ‬Ⴒ̹ͦ༦૶
ో . ঘཌ৪͈ఱఉତ̦ 5 पո‫͈ئ‬ঊ‫ ރ‬.

଎ˎȇ෎ఝ෎ζρςͺ SERA ͈ίυΓΏϋΈ౯༌͂ SE36 ΗϋΩ·ৗ͈ࢹ௮

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଎ːȇSE36 ζρςͺχ·Ιϋহࡑୋस :Ȫ़ȫिఱ๷୆໤ພࡄ‫ݪ‬ٛ۷‫إ‬঳ࡄ‫ݪ‬ਫ਼̤̞̀ͅ
GMP ૄ࠯ͬਖ਼৿̱̀୆ॲ̯̹ͦ .

଎ˏȇ෎ఝ෎ζρςͺࡔಖ͈୆‫۪͂ڰ‬χ·Ιϋ࢓༞ࢯࡔȪˎȫζρςͺࡔಖ͈ૺ‫ًا‬೾͈ٜྶȁζρςͺࡔಖ͉Ȃ̞ࣞ਽৽අ։଻Ȃໝॠ̈́୆‫۪ڰ‬Ȃ਽৽྾‫ע‬؊൞͈ٝ๰‫̓̈́ࢹܥ‬Ȃࣞഽͅૺ‫̹̱ا‬ξΣȜ·̈́අಭͬତ
ఉ̩ခ̳ͥȃ̭͈ͦͣ‫ܖ‬๕͂̈́ͥ໦ঊ‫̹̳̥ͥͣͅྶͬࢹܥ‬͛ͅȂ‫ړ‬ΊΦθȂ΂σ΄ΥρΊΦθ͈ٜඋ͞ࢯࡔ໦ঊ͈ఉ߿
ٜଢ଼̈́̓Ȃ֒ഥঊ෻Ⴅૂ༭̥͈ͣͺίυȜΙ࣐̞ͬ̽̀ͥȃ̭͈ͦͣࡄ‫͉ض଼͈̥ͣݪ‬χ·Ιϋ‫ٳ‬อ͈࡛ા́͜ခ࢘ͅ၌
ဥ̯̞ͦ̀ͥȃ଎ˑȇͺά΋ίρΑΠΊΦθͅ΋ȜΡ̯̞ͦ̀ͥ 30 ֒ഥঊͥ͢ͅࠏൡਏ :ΪΠͬ਽৽̳͂ͥ 4 ਅ͈ζρςͺࡔಖ͉ౙࠏൡ߲ͬࠁ଼̱̞̈́ȃ਽৽ഢ̦۟ૺ‫ً͈ا‬೾̤̞̀ͅ‫ة‬ഽ͜୆̲̞̀ͥȃड߃͈యນഎ̈́ა໲
1. Arisue N, Hashimoto T, Mitsui H, Palacpac NM, Kaneko A, Kawai S, Hasegawa M, Tanabe K, Horii T. The
Plasmodium apicoplast genome: conserved structure and close relationship of P. ovale to rodent malaria parasites.
Mol Biol Evol. 2012 Mar 6. (in press)
2. Tanabe K, Arisue N, Palacpac NM, Yagi M, Tougan T, Honma H, Ferreira MU, Färnert A, Björkman A, Kaneko A,
Nakamura M, Hirayama K, Mita T, Horii T. Geographic differentiation of polymorphism in the Plasmodium
falciparum malaria vaccine candidate gene SERA5. Vaccine. 2012 Feb 21;30(9):1583-93.
3. Palacpac NM, Arisue N, Tougan T, Ishii KJ, Horii T. Plasmodium falciparum serine repeat antigen 5 (SE36) as amalaria vaccine candidate. Vaccine. 2011 Aug 11;29(35):5837-45.
4. Arisue N, Kawai S, Hirai M, Palacpac NM, Jia M, Kaneko A, Tanabe K, Horii T. Clues to evolution of the SERA
multigene family in 18 Plasmodium species. PLoS One. 2011 Mar 15;6(3):e17775.
5. Horii T, Shirai H, Li J, Ishii KJ, Palacpac NM, Tougan T, Hato M, Ohta N, Bobogare A, Arakaki N, Matsumoto Y,
Namazue J, Ishikawa T, Ueda S, Takahashi M. Evidences of protection against blood-stage infection of Plasmodium
falciparum by the novel protein vaccine SE36. Parasitol Int. 2010 Sep;59(3):380-6.
ĴĴࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜ

;ͼσΑ྾‫ע‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ୆നȁგၻ੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁࣱଌȁ࣠੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁളᰦȁဢ໹අහ੩‫ޗ‬ȁȁ༗࠲‫ڠ‬ฎআȁऎș࿐ȁୃఱඅහ੩‫ޗ‬ȁȁ֓‫ڠ‬ฎআȁȁஃ֔ȁ̜̯̿අහࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁȁ֔ષȁဎ঄අහࡄ‫֥ݪ‬ȁ਷֓‫ڠ‬ฎআȁ߄֔ȁဈఊඅහࡄ‫֥ݪ‬ȁ࿪‫ڠ‬ฎআȁȁୌఆȁ࢕ࢩȁ൚໦࿤͉́Ȃ྾‫ࠏע‬Ȃࡤ‫ۜͅࠏܕݟ‬அ̳ͥ;ͼσΑ͞૰ࠐࠏͬ։ુ‫̳ͥا‬ίς΂ϋ̞̾̀ͅȂۜஅ‫̥ͣࢹܥ‬ພఠٜଢ଼Ȃ̷͈ଷࢄ༹‫ٳ‬อȂ̯͉ͣࠬͅ‫ס‬ୋसͅईව̱̹̭ͦͣພࡔఘ͈ෳੰ༹͞;ͼσΑۜஅછ͈ଇ௸૷౯༹͈‫ٳ‬อ࣐̞ͬ̽̀ͥȃȪˍȫ྾‫ۜͅࠏע‬அ̳ͥ;ͼσΑȁΟϋΈ৖͉ۛ෎ఝȆՏ෎ఝ౷֖͈ड͜ਹါ̈́;ͼσΑۜஅછ́Ȃٍ෾ٚ଻ͅȂ྾‫͈ࠏע‬ळ༾ۜͅஅ̳ͥȃΟϋΈ
;ͼσΑ͉ˍ߿ȡː߿͈ࠬୄ߿ͅ໦̥ͦȂˎٝ࿒ͅˍٝ࿒͉͂։̈́ͥࠬୄ߿;ͼσΆۜஅ̱̹ાࣣ͉ͅΟϋΈ੄ࠬ෎͞ΟϋΈΏοΛ·છ࢓߲̈́̓ਹછ‫ͬا‬ઉ̩̭̦͂ఉ̞ȃۜஅ৪͉‫̞ޑ‬ಎგࢯఘͬॲ୆̳̦ͥȂ̭͈ࢯఘ͈ಎͅ
͉Ȃষۜͅஅ̱̹Ȃࠬୄ߿͈։̈́ͥ;ͼσᾼచ̱͉̀ۜஅ௩‫ͅ଻ޑ‬൱̩ࢯఘ͜ంह̳ͥ͂࡞̞ͩͦ̀ͥȃ̭͈‫ب‬୰ͤ͢ͅȂχ·Ιϋ‫ٳ‬อ̦ࣾඳণ̯̞ͦ̀ͥȃজ̹̻͉ȂΟϋΈ;ͼσΑ͈ۜஅ̧̭̯ͤ֨ܳͦͥ͢ͅພఠ‫ܥ‬੬͈ٜྶȂ̯ͣͅ;ͼσΑໝୋဲଷͅ൱̩Ȃခ࢘̈́হၷ࿪͈‫ٳ‬อͬૺ̞͛̀ͥȃ̷͈ບ‫̹͈͛ͅث‬ȂΟϋΈ;ͼσᾼۜ਋଻͈൲໤κΟσ͈‫ٳ‬อ͜ૺ̞͛̀ͥȃ̹͘ȂΟϋΈۛ৪ဇြ྾‫ע‬ळ༾ͬဥ̞̹ΪΠ߿ಎგ଻ౙ·υȜϋࢯఘͬैୋ̱Ȃ̭ͦͣͬဥ̞̹ພఠ‫ܥ‬੬ٜྶ͂‫ࢯͅވ‬ఘ֓࿪͈‫ٳ‬อ͜࿒ঐ̱̞̀ͥȃȪˎȫࡤ‫ۜͅࠏܕݟ‬அ̳ͥ;ͼσΑȁͼϋέσ΀ϋΎ;ͼσΑ͉Ȃങ߿എ̈́‫ۜܕݟࡤ͈଻ݢ‬அછ̧̭̳ͬ֨ܳȃ‫ܬ‬୯଻ͼϋέσ΀ϋΎ;ͼσᾼ‫̢ح‬Ȃ
2009 ාͅήῌဇြ̳ͥ૧߿ͼϋέσ΀ϋΎ;ͼσΑ̦૧̹ͅ੄࡛̱Ȃ࡛हȂࣞພࡔ଻ುͼϋέσ΀ϋΎ;ͼσᾼဇြ̳ͥ૧߿ͼϋέσ΀ϋΎ;ͼσΑ͈੄࡛̦࠼ැ̯ͦȂଲ‫ٮ‬എ̈́‫۾‬૤ম̞̈́̽̀ͥͅȃজ̹̻͉Ȃͺτ΅΍ϋΡςͺఱ‫ވ͂ڠ‬൳́Ȃ΀ΐίΠ́ၠ࣐̳ͥࣞພࡔ଻ುͼϋέσ΀ϋΎ;ͼσΑ H5N1 Տ߿̞̾̀ͅࡄ‫̀͛ૺͬݪ‬̞ͥȃজ̹̻͉Ȃ߃ා൳࣭́ၠ࣐̳ͥ H5N1 ;ͼσΑ͈ಎͅȂΪΠ߿τΓίΗȜൠङ̭̞ࣣͦ́ͤࣞࠫ͘͢ͅ૶გ
଻ͬ‫ڕ‬ං̱̹૧̱̞;ͼσΑ߲̦੄࡛̱̞̭̀ͥ͂ͬྶ̥̱̞ͣ̀ͥͅȃ΀ΐίΠ̤̫ͥͅ H5N1 ;ͼσΑဇြΩϋΟηΛ·;ͼσΑ੄࡛͈ஆह଻̷͈͂‫ܥ‬੬͈ٜྶͬদ͙̞̀ͥȃ̹͘Ȃͼϋέσ΀ϋΎ;ͼσᾼచ̱̀ಎგ‫ڰ‬଻ͬা̳ΪΠ߿ౙ·υȜϋࢯఘͬैୋ̱Ȃ̭ͦͣͬဥ̞̹ࢯఘ֓࿪͈‫ٳ‬อͬ࿒ঐ̱̞̀ͥȃ̯ͣͅȂࣝࢽ߿ͼϋέσ΀ϋΎ;ͼσᾼచ̱̀ࢩ̩ಎგ̳ͥౙ·υȜϋࢯఘ͈෇ে΀άΠȜίႀ֖̦ࣞ༗ం଻͈ࣞষࢹ௮ͬࠁ଼̳ͥႀ
֖̜̹̭̥́̽͂ͣȂ̭͈ࣞষࢹ௮ͬਹণ̱̹ষଲయ߿΀άΠȜίχ·Ιϋ͈‫ٳ‬อͬȂܑު͈͂‫ވ‬൳ࡄ‫ૺ̱̀͂ݪ‬̞͛̀ͥȃȪˏȫࠬ‫ס‬ୋस̱ͬٚ̀ۜஅഥ෥̳ͥ;ͼσΑȁࠬ‫סࠬ͞ס‬ୋस͈հ஠଻‫ږ‬༗͈̹͛ͅȂ̭ͦͣୋस̱ͬٚ̀ۜஅഥ෥͈ςΑ·͈̜ͥΩσδ;ͼσΑ B19ȂSARS ΋υ΢;ͼσΑȂE ߿۴‫ͼ;׶‬σΑȂίς΂ϋ൝ͅ‫̳ͥ۾‬ෳੰܑ༹̞̾̀ͅު͈͂‫ވ‬൳ࡄ‫࠿̱̀͂ݪ‬൦̱̞̀ͥȃȪːȫۜஅછ͈ଇ௸૷౯༹͈‫ٳ‬อȁ̯̰̈́͘͘;ͼσΑۜஅછ͈૷౯͉ͅȂࠖ࢕ࢯఘ༹ȂELISAȂ;ͿΑΗϋήυΛΠ༹̈́̓ͬဥ̞ͥࠬୄ‫ڠ‬എ਀༹͞ PCR ͬဥ̞ͥ໦ঊഎ਀༹̦न̞ͣͦ̀ͥȃজ̹̻͉Ȃۜஅછ͈ଇ௸૷౯༹̱͈͂̀ͼθΦ·υζΠ༹ͥ͢ͅ΅ΛΠ‫ٳ‬อܑͬު͈͂‫ވ‬൳ࡄ‫̞࣐̱ͥ̀̽̀͂ݪ‬ȃĴĵ

NS2BȪέτȜθࢹ௮ȫ͉ NS3Ȫႂȫ͈εΉΛΠ໐պ͂௖ࡽैဥ̱̤̀
ͤȂάϋ·໐໦͉Ȃজ̹̻̦૧̹ͅ
ࡉ੄̱̹೩໦ঊ͈͂௖ࡽैဥͅਹါ̈́ͺηΦॸॼ‫ͬܖ‬া̱̞̀ͥȃ

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‫ڰ‬
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͈
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ΟϋΈ;ͼσᾼུ͉̦̹֚̾̈́̽εςίυΞͼῧ̱̀ॲ୆̯ͦ
ͥȃ;ͼσΑၥঊ͈ࠁ଼͈̹͛ͅȂ10 ࡢ͈ΗϋΩ·ৗͅ୨౯̯ͦ
̭̦ͥ͂ຈଌ̜́ͥȃΟϋΈ;ͼσΑίυΞͺȜΔ NS3 ً͉̭͈೾ͅຈଌ͈ΗϋΩ·ৗ̜́ͤȂCofactor ̜́ͥ NS2B ͂ໝࣣఘͬࠁ
଼̱‫ͬ଻ڰ‬อ‫̳ͥܞ‬ȃਲ̽̀ȂNS3 ͂ NS2B ͈͂௖ࡽैဥͬஷ‫̳ٺ‬ͥ೩໦ঊ͉Ȃ;ͼσΑໝୋͬஷ‫̳ͥٺ‬൱̧̳ͬͥȃ

ΟϋΈ;ͼσΑۜஅ͉‫ࢯ̞ޑ‬ఘॲ୆ͬည൵̳ͥȃ̭ͦͣࢯఘ͉ۜͅ
அଷࢄͅ൱̩ಎგࢯఘ͈͙͉̩́̈́Ȃౙ‫ ݩ‬/ ζ·υέ͹Ȝΐͅอ࡛̱̞̀ͥ Fc τΓίΗȜ̱ͬٚȂࢯఘջం଻͈ۜஅ௩‫̧ܳ֨ͬ଻ޑ‬̭̳ࢯఘ͜ంह̳ͥ͂࡞̞ͩͦ̀ͥȃۜஅ৪ဇြ྾‫ע‬ळ༾ͬဥ̞̀
ैୋ̱̹ΪΠ߿ౙ·υȜϋࢯఘͬဥ̞̀Ȃࢯఘջం଻͈ພఠ‫ܥ‬੬͈ٜྶ৾ͤͅழ͚͂‫ͅވ‬Ȃ࢘‫ض‬എ̈́হၷဥࢯఘ͈‫ٳ‬อ͜࿒ঐ̱̞̀ͥȃ߃ා΀ΐίΠ́ၠ࣐̳ͥࣞພࡔ଻ುͼϋέσ΀ϋΎ;ͼσΑ H5N1
Տ߿͈ಎͅȂΪΠ͈‫ئ‬໐ࡤ‫ܕݟ‬ષ๧̭̞ͦ́ͤࣞ͘͢͜ͅ‫ݟ‬಍଻Ȇۜஅ଻ͬ‫ڕ‬ං̱̹;ͼσΑ߲̦੄࡛̱̞̀ͥȪৢ૯ȇ୤૗̦ΪΠ͈‫ۯܨ‬ષ๧ͅ‫ݟ‬಍̱̹΀ΐίΠၠ࣐‫ͬڼ‬া̳ȫȃ൳࣭̤̫ͥͅ H5N1;ͼσΑဇြΩϋΟηΛ·;ͼσΑ੄࡛͈ஆह଻̷͈͂‫ܥ‬੬͈ٜྶͬদ͙̞̀ͥȃ(a) ങ߿എ̈́ H5N1 ;ͼσΑ͈ۜஅࠁఠȃ;ͼσΑ̦‫ئ‬໐ࡤ‫́ܕݟ‬ໝୋ̳̭ͥ͂́੝͛̀ພࡔ଻̦อ‫̯ͥͦܞ‬ȃ(b) ߃ා΀ΐίΠ́ၠ࣐̱̞̀ͥ૧̱̞ H5N1 ;ͼσᾼ߲ͥۜ͢அ
ࠁఠȃ̭ͦ́ͤ͘͢ࢩํ̈́ࡤ‫ܕݟ‬໐պͅచ̱̀Ȃͤ͢ઁ̞̈́
;ͼσΑၾ́ۜஅ଼̦ၛ̱̠ͥ͂ࣉ̢ͣͦͥȃొ̱Ȃ̭ͦ́͘΀ΐίΠ̤̞̀ͅȂH5N1 ;ͼσΑ͈ࡢఘ‫ۼ‬ഥ෥͉‫̯෇ږ‬̞̞ͦ̀̈́ȃ

ड߃͈యນഎ̈́ა໲
1. Yasugi M, Nakamura S, Daidoji T, Ramadhany R, Yang C-S, Yasunaga T, Iida T, Horii T, Ikuta K, Takahashi K,
Nakaya T. Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in
Japan between 2009 and 2010. PLoS One 2012;7(2):e30946. Epub 2012 Feb 17.
2. Watanabe Y, Ibrahim MS, Suzuki Y, Ikuta K. The changing nature of avian influenza A virus (H5N1). Trends
Microbiol. 2012 Jan;20(1):11-20. Epub 2011 Dec 5.
3. Watanabe Y, Ibrahim MS, Ellakany HF, Kawashita N, Mizuike R, Hiramatsu H, Sriwilaijaroen N, Takagi T, Suzuki
Y, Ikuta K. Acquisition of human-type receptor binding specificity by new H5N1 influenza virus sublineages during
their emergence in birds in Egypt. PLoS Pathog. 2011 May:7(5):e1002068. Epub 2011 May 26.
4. Mizuike R, Sasaki T, Baba K, Iwamoto H, Shibai Y, Kosaka M, Kubota-Koketsu R, Yang CS, Du A, Sakudo A,
Tsujikawa M, Yunoki M, Ikuta K. Development of two types of rapid diagnostic test kits to detect the hemagglutinin
or nucleoprotein of the swine-origin pandemic influenza A virus H1N1. Clin. Vaccine Immunol. 2011 Mar;18(3):494-9. Epub 2011 Jan 12.
5. Kurosu T, Khamlert C, Phanthanawiboon S, Ikuta K, Anantapreecha S. Highly efficient rescue of dengue virus using
a co-culture system with mosquito/mammalian cells. Biochem Biophys Res Commun. 2010 Apr 2;394(2):398-404.
Epub 2010 Mar 7.
ĴĶࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities֒ഥૂ༭৘ࡑΓϋΗȜ֒ഥঊ‫ܥ‬ෝٜଢ଼໦࿤
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ġĩ ࠳ Īġ੎‫ޗ‬਎ġ
੎‫ޗ‬਎ġĩ ࠳ Īġ੩‫ޗ‬ġ࿪‫ڠ‬ฎআȁ‫ؖ‬໐ȁੳၑ‫ڠ‬ฎআȁ२ႊȁ‫ڡ‬঎࿪‫ڠ‬ฎআȁչ୼ȁୃ૽֓‫ڠ‬ฎআȁႱႊȁ‫ܧם‬੩‫ޗ‬ġ࿪‫ڠ‬ฎআȁȁȁ֔ષȁೄგ੩‫ޗ‬ġĩ ࠳ Īġ࿪‫ڠ‬ฎআȁȁȁ֙౓ȁիঊඅහ੩‫ޗ‬ġĩ ࠳ Īġ ୆ྵ‫ڠش‬ฎআȁऎ൥ȁဉ࢖ȁΪΠ͞ζ;Α͈ΊΦθίυΐͿ·Π̦֚؊͈ۖၭ̢̹࡛ͬࠞह͉́Ȃ૽ࢥഎ֒ͅഥঊͬௌै̱̹֒ഥঊழ̢۟൲
໤̦Ȃ৖ພ͈ࡄ‫ܖ͞ݪ‬யഎ̈́୆໤‫ͅݪࡄڠ‬ਹါ̈́࿨‫̞̠̳̹ͥ̀̽̈́͢ͅضͬڬ‬ȃِș͉ࡢఘτασ͈́֒ഥঊ‫ܥ‬ෝٜଢ଼ΜȜσͬ‫ٳ‬อ̱̀୆૓୆໤‫ڠ‬໦࿤͈́ࡄ‫̠࣐͂͂ͬ͜ͅݪ‬Ȃ֒ഥঊழ̢۟൲໤͈ैୋ঑‫̞࣐ͥ̀̽ͬ׳‬ȃࡄ‫ݪ‬ඤယ
ȁِș͉ଲ‫̫̀ߐ୶ͅٮ‬อ࢕΂χϋ·ρΊ͈ GFP ֒ഥঊͬழ͙ࣺ̺ͭࠖ࢕ζ;Αͬैୋ̳ͥ͂͂͜ͅȂ̷͈Φ;
Χ;ͬ୆̥̱̀਋ୈ͈ι΃ΣΒθ͞୆૓ळ༾଼͈ͤၛ̻ͬࡄ‫̞̱ͥ̀ݪ‬Ȫ଎ˍȫȃ̭ͦ́͘ͅୈ௏අ։എ̈́઀༾ఘ
Ώλβυϋ߲ȪCLGN, CALR3, PDILTȫ͈ΦΛ·ͺ;Πζ;Αͬैୋ̱Ȃ̷̦ͦͣୈঊ྄ΗϋΩ·ৗȪADAM3ȫ͈຦ৗ‫ۯ‬ၑͅຈါ̜̭́ͥ͂Ȃࠫ‫ ̱̀͂ض‬ADAM3 ͬ৐̹̽ୈঊ͉ঊ‫̥ͣݠ‬၅‫̧̹̞࣐͛̈́́ͅ֊ͅۯ‬ဎ଻ະළ̭͂̈́ͥ͂ͬྶ̥̱̹ͣͅȪ଎ˎȫȪˍȫȃȁ̭͂ͧ́Ȃ၅‫ͅۯ‬ൢో̱̹ୈঊ͉၅ঊͬ৾ͤս̞ͭ́ͥळ༾‫ٸ‬ζΠςΛ·ΑȪ൫ྶఝȫࣣ̱ࠫ̀ͅ୶ఘ฽؊̱Ȃ൫ྶఝͬ೒ً̳ͥ͂૞̲̞ͣͦ̀ͥȃ̱̥̱ȂADAM3 ͬ৐̽̀൫ྶఝ̧ࣣ̞ࠫ́̈́ͅୈঊ́͜၅‫ݚ‬ळ༾̦ంह̳
ͦ͊൫ྶఝͬ೒ً̱̀਋ୈ̧̭́ͥ͂͞Ȃ൫ྶఝͬ೒ً̱̀୶ఘ฽؊ͬਞ̢̹ୈঊ̦ठഽ൫ྶఝͬ೒ً̧̭́ͥ͂
̥ͣȪ଎ˏȫȪˍ , ˎȫȂِș͉୶ఘ฽؊ஜ͈ୈঊ͂൫ྶఝ͈ࣣ͉ࠫͅ཰̓փྙ̦̞̈́͂ࣉ̢̤̀ͤȂ਋ୈ͈ΩρΘͼθͬ੥̧̢͓̩۟ͥࡄ‫ͬݪ‬ਹ͇̞̀ͥȃఈ͜ͅΦΛ·ͺ;Πζ;Αͬဥ̞̹ٜଢ଼̥ͣȂୈঊ͈୶ఘඤ྄͈IZUMO1 ̦၅ঊ͈͂ဏࣣͅຈଌ̜̭́ͥ͂͞Ȫ଎ːȫȂSPESP1 ̦ဏࣣͅຈଌ̈́ୈঊ྄ࢹ௮͈հ೰‫ͅا‬ਹါ̜́ͥ
̭͂̈́̓ͬ༭̱̞࣬̀ͥȃ଎ˍȫ൮໐͈୶ఘ̦ GFPȂ๶໐͈ηΠ΋ϋΠςͺ̦ RFP ́ρασ̯̹ͦΠρϋΑΐͿΣΛ·ζ;Αୈঊȃ୶ఘ฽؊̳ͥ͂ୈঊ͉୶ఘඤ͈ GFP ͬ༶੄̱̀
ࠖ࢕ͬ৐̠ȃ

଎ˎȫADAM3 ̦ક৐̱̹ Pdilt ΦΛ·ͺ;Πζ;Α͈ୈঊ͉ঊ‫̥ͣݠ‬၅‫̴̧࣐́֊ͅۯ‬Ȃဎ଻ະළ͈ࡔ֦͂̈́ͥȃ଎ˏȫ૽ࢥഎ৾ͤͅ੄̱̹ս၅͈࣌ୈঊ͉Ȃ༆͈၅ঊ͈൫ྶఝͬ೒ً̱̀਋ୈ̧́ͥȃ଎ːȫIzumo1 ΦΛ·ͺ;Πζ;Α͈ୈঊ͉൫ྶఝͬ೒ً̱̀ս၅࣌ඤͅ૟
ව̧̦́ͥȂ၅ঊͅဏ̧ࣣ̞́̈́ȃĴķȁِș͉୆૓ࢥ‫ڠ‬Ȇอ୆ࢥ‫̹̱ঀߐͬڠ‬ͺίυȜΙ̥ͣ୆ྵ͈आ࡙ͅทͥΙλτϋΐϋΈ̈́ࡄ‫ͬݪ‬࿒ঐ̱̞̀ͥȃ
τϋΙ;ͼσΑα·ΗȜͬဥ̞̭ͥ͂́Ȃథ঱֒ͅഥঊͬ൵ව̳̭̩ͥ͂̈́థ๕͈͙֒ͅഥঊௌै̧༹༷́ͥͬࣉ
մ̳ͥ͂͂͜ͅȂథ๕අ։എͅ sFLT1 ͬอ࡛̳̭ͥ͂́ළૡࣞࠬգછ࢓߲͈κΟσζ;Αͬ‫ٳ‬อ̱̞̀ͥȪˏȫȃ

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ड߃͉Ȃඊুͅਏၛ̱̹ρΛΠ ES ळ༾ͬဥ̞̀ζ;Α↔ρΛΠ΅ιρ൲໤ͬဥ̞̹௫‫ܕ‬ठ୆֓‫͞ݪࡄڠ‬Ȫ଎ˑȫȪːȫȂmiRNA ࠧఅζ;Αͬဥ̞̹ non coding RNA ͈୆ఘ‫ܥ‬ෝٜଢ଼̈́̓৾ͤͅழ̞ͭ́ͥȃ଎ˑȫζ;Α͈᥷๕༾ͅρΛΠ ES ळ༾ͬಕව̱̀ैୋ̱̹ζ;Α↔ρΛΠ ΅ιρȃฒ૗໐໦̦ζ;ΑȂခ૗໐໦̦ρΛΠဇြ͈ळ༾̜́ͥȃࡄ‫ݪ‬঑‫׳‬ȁۜஅ൲໤৘ࡑঔ୭͂‫ވ‬൳̱̀Ȃ‫ڠ‬ඤ̴͈͙̈́ͣࢩ̩‫֒͜ͅٸڠ‬ഥঊழ̢۟൲໤͈ैୋ঑‫̞࣐ͥ̀̽ͬ׳‬ȃΦΛ·
ͺ ; Π ζ ; ΑȂΠ ρ ϋ Α ΐ Ϳ Σ Λ · ζ ; Α ͉ ̷ ͦ ̸ ͦ 100Ȃ300 ρ ͼ ϋ ͬ ಼ ̢ ͥ ै ୋ ৘ ୡ ̦ ̜ ͥȪhttp://www.tgko.biken.osaka-u.ac.jp/tgko/sum/indexȫȃ̯ͣ֒ͅഥঊழ̢۟൲໤ͬਹါ̈́ࡄ‫ݪ‬঩࡙̱͂̀༗ం̳ͥ࿒
എ́Ȃ਋ୈ၅͞ୈঊ͈ൄࠫ༗ం͜঑‫̞̱ͥ̀׳‬ȃࡄ‫ݪ‬ඤယȪ२ႊΈσȜίȫȁ੏۪‫ࠏܕ‬৖ۛͅ‫۾‬ဓ̳ͥ֒ഥঊ̞̾̀ͅ໦ঊ୆໤‫ڠ‬എٜଢ଼࣐̞ͬ̽̀ͥȃ૤ະ஠͈࿩฼ତͬ୸͛ͥ‫ڐ‬ಫະ஠͈ພ
ఠ͈ٜྶͬ૙‫ۜ؂‬਋଻ࣞࠬգΘȜσρΛΠκΟσैୋ̱Ȃ̷͈อછι΃ΣΒθٜͬଢ଼̱Ȃࣞࠬգۛ৪́௩‫̳ࠬͥح‬ୄඤ֦଻ΐΆΗςΑအ໤ৗ͈ Na+/Ca2+ exchanger ͅచ̳ͥ‫ޣג‬Ȫˑȫ͞૙‫ۜ؂‬਋଻֦ঊ̱͂̀΃σΣΙϋ̦૤ऒ৒஌֋‫ဲ͈ا‬ଷͥ͢ͅऒ৒‫ڐ‬ಫ‫ܥ‬ෝ͈٨஝ͤ͢ͅ‫ڐ‬ಫະ஠͈อછ̦ဲଷ̯̹̭ͦ͂ͬࡉ੄̱̞̀ͥȪ଎˒ȫȃ(a)Control

HFpEF

Carnitine଎˒ȫ20 ਩႓ΘȜσρΛΠ̤̫ͥͅऒ૤৒͈஌֋‫ا‬ȃ
EFpEF; ‫ڐ‬ಫະ஠ેఠȂCarnitine; ΃σΣΙϋͬൎဓࢃȃ

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ड߃͈యນഎ̈́ა໲
1. Tokuhiro K, Ikawa M, Benham AM, Okabe M. Protein disulfide isomerase homolog PDILT is required for quality
control of sperm membrane protein ADAM3 and male infertility. Proc Natl Acad Sci U S A. 2012 March 6; 109(10): 3850-3855.
2. Inoue N, Satouh Y, Ikawa M, Okabe M, Yanagimachi R. Acrosome-reacted mouse spermatozoa recovered from theperivitelline space can fertilize other eggs. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20008-11.
3. Kumasawa K, Ikawa M, Kidoya H, Hasuwa H, Saito-Fujita T, Morioka Y, Takakura N, Kimura T, Okabe M.
Pravastatin induces placental growth factor (PGF) and ameliorates preeclampsia in a mouse model. Proc Natl Acad
Sci U S A. 2011 Jan 25;108(4):1451-5.
4. Isotani A, Hatayama H, Kaseda K, Ikawa M, Okabe M. Formation of a thymus from rat ES cells in xenogeneic nude
PRXVHļUDW(6FKLPHUDVGenes Cells. 2011 Apr;16(4): 397-405.
5. Kamimura D, Ohtani T, Miwa T, et al.Ca2+ Entry mode of Na+/Ca2+ exchanger as a new therapeutic target forheart failure with preserved ejection fraction. European Heart J. 2011 Apr 13.
Ĵĸࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities֒ഥૂ༭৘ࡑΓϋΗȜΊΦθૂ༭ٜଢ଼໦࿤
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎ġၑ‫ڠ‬ฎআġհ‫ז‬ȁચဎ
‫ޗ‬਎Ȫ࠳ȫġ࿪‫ڠ‬ฎআġ
ࣞ࿐ȁో࿟
੩‫ޗ‬ġ
ฎআȪၑ‫ڠ‬ȫġ ࢃ൥ȁೄ‫ݛ‬੩‫ޗ‬ġฎআȪ࿪‫ڠ‬ȫġ ಎఆȁઌఊ੩‫ޗ‬Ȫ࠳ȫġ ฎআȪ࿪‫ڠ‬ȫġ ୼‫ئ‬ȁၑ඾૽අහࡄ‫֥ݪ‬ġ ฎআȪၑ‫ڠ‬ȫġ ५‫ئ‬ȁྶঃඅහࡄ‫֥ݪ‬ġ ฎআȪၑ‫ڠ‬ȫġ ŖįġńũŢůťŪŮŢŭġťŦġŔŪŭŷŢ

ȁ൚໦࿤͉́Ȃఱ߿ࠗॳ‫̱ঀߐͬܥ‬Ȃఱၾ͈֒ഥঊȆΊΦθૂ༭ͅచ̳ͥఱܰ࿅̥̾࿌္എٜ̈́ଢ଼̥ͣ୆ྵ࡛ય͞
୆໤͈ૺ‫͈ا‬ٜྶͬ࿒ঐ̳ࡄ‫̞࣐ͥ̀̽ͬݪ‬ȃ̹͘ȂΨͼ΂ͼϋέ΁ζΞͻ·Α͞໦ঊ୆໤‫ڠ‬ဥ͈ΕέΠ;Ϳͺ͈‫ٳ‬อ࣐̞ͬ̽̀ͥȃ̯ͣͅȂΊΦθૂ༭ٜଢ଼ဥ΋ϋάνȜΗΏΑΞθͬ‫׋‬ဥ̱Ȃ‫ڠ‬ඤ͈֒ഥঊȆΊΦθ‫ݪࡄ߸۾‬৪ࠗͅॳ‫ܥ‬঩࡙ͬ೹‫̳͂͂ͥ͜ͅރ‬ȂΊΦθૂ༭ٜଢ଼͞΋ϋάνȜΗΏΑΞθ͈၌ဥ༹༷̞͈̾̀ͅ‫ࡄ֗ޗ‬ਘ࣐̞ͬ̽̀ͥȃȪˍȫఱܰ࿅ΊΦθૂ༭ٜଢ଼ȁ࡛ह́͘ͅତఉ̩͈୆໤ਅ͈ΊΦθ෻Ⴅ̦ࠨ೰̯̞̦ͦ̀ͥȂ̭͈ͦͣΊΦθ෻Ⴅ̷ͦ͞ͅັଟ̳ͥ֒ഥঊȂΗ
ϋΩ·ৗȂRNA ͈̈́̓ૂ༭̞̾̀ͅȂΨͼ΂ͼϋέ΁ζΞͻ·Α͞໦ঊૺ‫̓̈́ڠا‬အș̈́਀༹ͬߐঀ̱̹࿌္എ
ٜ̈́ଢ଼࣐̞ͬ̽̀ͥȃ൳শͅȂఱܰ࿅ΊΦθૂ༭ٜଢ଼ͅຈါ̈́ΕέΠ;Ϳͺٜ͞ଢ଼ͺσΌςΒθ͈ࡄ‫ٳݪ‬อ࣐̞ͬ̽̀ͥȃِș͉༗ం෻Ⴅࠨ೰ΕέΠ;Ϳͺ CONSERV ͬ‫ٳ‬อ̱Ȃळ޿̥ͣΪΠͅঢͥအș̈́୆໤ 266 ਅ͈ΊΦθ෻Ⴅ͈ٜଢ଼̥ͣȂ͕̳͓͈͂ͭ̓̀୆໤͈ΊΦθͅ༗ం̯̞ͦ̀ͥະ་෻Ⴅͬࡉ੄̱̹ (Goto et al. 2007)Ȫ଎ˍȫȃ̹͘Ȃͼϋέσ΀ϋΎ;ͼσΑ͈ૺ‫ͬا‬࿌္എ̈́ΊΦθ෻Ⴅٜଢ଼́೏̞̽̀ͥȪ଎ˎȫȃ଎ˍȁ266 ਅ͈ΊΦθ෻Ⴅٜଢ଼ͤ͢ͅྶ̥̹ͣ͂̈́̽
஠୆໤͈ΊΦθ͈༗ం෻Ⴅ

ĴĹ଎ˎȁͼϋέσ΀ϋΎ;ͼσΑΊΦθ͈࿌္എ̈́ૂ༭ٜଢ଼

ȪˎȫষଲయΏȜ·΀ϋ΍Ȝͥ͢ͅΊΦθૂ༭ٜଢ଼ȁ߃ා৘ဥ‫̹̯ͦا‬ষଲయΏȜ·΀ϋ΍Ȝ͉́Ȃ1 ୆໤͈ΊΦθ஠ఘͬࠨ̭͛ͥ͂͜‫خ‬ෝ͕̈́̓ఱၾ͈‫ܖ؂‬෻Ⴅ֚ͬഽͅࠨ೰̧̳̭̦ͥ͂́ͥȃ൚ࡄ‫ݪ‬৒͉ষଲయΏȜ·΀ϋ΍Ȝ̦੄ႁ̳ͥཛྷఱ̈́ΟȜΗၾ͈ΊΦθૂ༭ٜଢ଼ͅచ

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‫ݪ‬
‫ڰ‬
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؊̳͓̩Ȃٜଢ଼ΕέΠ;Ϳͺ͈‫ٳ‬อ̤͍ٜ͢ଢ଼ΏΑΞθ͈ࢹಃ࣐̞ͬ̽̀ͥȃࢹಃ̱̹ٜଢ଼ΏΑΞθͬဥ̞̀Ȃພࡔ๷୆໤̈́̓အș̈́୆໤͈૧ܰΊΦθ෻Ⴅࠨ೰͞๤‫ڛ‬ΊΦθٜଢ଼ͬ๷୆໤ພࡄ‫ݪ‬ਫ਼ඤ͞‫ڠ‬ඤ‫ݪࡄڎ͈ٸ‬৒͂‫ވ‬൳࣐̞́̽̀ͥȪ଎ˏȫȃȪˏȫ‫ڠ‬ඤ‫ވ‬൳၌ဥ͈ΊΦθૂ༭ٜଢ଼ဥ΋ϋάνȜΗΏΑΞθ͈‫׋‬ဥȁΊΦθૂ༭ٜଢ଼ဥ͈ࠗॳ‫ܥ‬ΏΑΞθȪ଎ːȫͬ‫׋‬ဥ̱Ȃ‫ڠ‬ඤ͈၌ဥ৪͒೹‫̞̱ͥ̀ރ‬ȃུΏΑΞθ͉́Ȃ֒ഥૂ༭ٜଢ଼ͅຈଌ͈ଲ‫ٮ‬ಎͅ࢖‫̞̯ͥ̀ͦٳ‬အș̈́ΟȜΗαȜΑͬව਀̱Ȃુͅड૧͈ેఠͅু൲എͅ༗̹̠ͦͥ͢֋
঵‫ۯ‬ၑ̱̦̈́ͣ၌ဥ৪ͅ೹‫̞̱ͥ̀ރ‬ȃ଎ˏȁষଲయΏȜ·΀ϋ΍Ȝͥ͢ͅΊΦθٜଢ଼ȃ
1 ͈ٝΏȜ·΀ϋΏϋΈ́ΊΦθ͈͕͖஠ႀ֖ͬ
ٜඋ̳̭̦ͥ͂‫خ‬ෝ଎ːȁ֒ഥૂ༭৘ࡑΓϋΗȜࠗॳ‫ܥ‬ΏΑΞθ͈ٜଢ଼΍ȜΨȜȪऒȫ͂ఱယၾΑΠτȜΐȪֲȫड߃͈యນഎ̈́ა໲
1. de Silva UC, Tanaka H, Nakamura S, Goto N, Yasunaga T. A comprehensive analysis of reassortment in influenza A
virus. Biol Open. 2012 Feb 23;1(4):385-90.
2. Yamashita A, Kawashita N, Kubota-Koketsu R, Inoue Y, Watanabe Y, Ibrahim MS, Ideno S, Yunoki M, Okuno Y,
Takagi T, Yasunaga T, Ikuta K. Highly conserved sequences for human neutralization epitope on hemagglutinin of
influenza A viruses H3N2, H1N1 and H5N1: Implication for human monoclonal antibody recognition. Biochem
Biophys Res Commun. 2010 Mar 19;393(4):614-8.
3. Nakamura S, Yang CS, Sakon N, Ueda M, Tougan T, Yamashita A, Goto N, Takahashi K, Yasunaga T, Ikuta K,
Mizutani T, Okamoto Y, Tagami M, Morita R, Maeda N, Kawai J, Hayashizaki Y, Nagai Y, Horii T, Iida T, Nakaya
T. Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput
sequencing approach. PLoS One. 2009;4(1):e4219.
4. Yamashita A, Goto N, Nishiguchi S, Shimada K, Yamanishi H, Yasunaga T. Computational search forover-represented 8-mers within the 5'-regulatory regions of 634 mouse testis-specific genes. Gene. 2008 Dec 31;427(1-2):93-8.
5. Goto N, Kurokawa K, Yasunaga T. Analysis of invariant sequences in 266 complete genomes. Gene. 2007 Oct
15;401(1-2):172-80.
Ĵĺࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities֒ഥૂ༭৘ࡑΓϋΗȜۜஅછιΗΊΦθࡄ‫ݪ‬໦࿤ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎Ȫ࠳ȫġၑ‫ڠ‬ฎআȁཱ֔ȁੇࢤ‫ޗ‬਎Ȫ࠳ȫġၑ‫ڠ‬ฎআȁհ‫ז‬ȁચဎඅහ‫ޗ‬਎Ȫ࠳ȫġ ֓‫ڠ‬ฎআȁ๑നȁഓ࿟੩‫ޗ‬Ȫ࠳ȫȁฎআȪၑ‫ڠ‬ȫȁࢃ൥ȁೄ‫ݛ‬੩‫ޗ‬Ȫ࠳ȫȁฎআȪ࿪‫ڠ‬ȫȁಎఆȁઌఊࡄ‫ݪ‬ඤယ
ˍȅRAPID (Robotics Assisted Pathogen IDentification)ȁ໲໐‫ڠش‬જ͈ȶۜஅછࡄ‫࣭ݪ‬षΥΛΠχȜ·ଔૺίυΈρθȷඤ̤̞̀ͅȂࡔ֦ະྶۜஅછ႕͈޽‫૷ݢ‬౯ȶRAPIDȷ͈ఘଷͬၑࡄ΂ηΛ·Α‫ܖ‬๕ࡄ‫ݪ‬ႀ֖͂‫ވ‬൳́ࢹಃ̱̞̀ͥȃͺΐͺȆͺές΃͈ 8 ύ࣭ͅै̹ͣͦۜ
அછࡄ‫ݶݪ‬ത͂Ⴒࠈ̱Ȃ޽‫ͬݢ‬ါ̳ͥͺ;ΠήτȜ·ΉȜΑ̥͈ͣພࡔఘ࠿੄ͬদ͙̞̀ͥȃˎȅ‫ڎ‬ਅۜஅછ͈ιΗΊΦηΛ·૷౯အș̈́ۜஅછͬιΗΊΦηΛ·ٜଢ଼́૷౯̧̥̠̥́ͥ̓Ȃ༹༷აٜ͞ଢ଼༹ͬࡄ‫̞̱ͥ̀ݪ‬ȃ̹͘૽਷‫ވ‬೒ۜஅછ͈୶࣐ࡄ‫̱̀͂ݪ‬൲໤ဇြদၳ̥ͣພࡔఘͬౝ̱̞॑̀ͥȃˏȅۜஅછอછশ͈ಸඤळ޿஽ٜଢ଼
ಸඤळ޿஽̦အș̈́৖̤̞ۛ̀ͅਹါ̈́࿨‫̦̜̥ͥ̾̾ͤ̈́ͣͅྶ̦̭̞̱̹͂ͥ̀ضͬڬ‬Ȃِș͉‫ئ‬ၓછอછশ͈ಸඤळ޿஽ͬࡄ‫̞̱ͥ̀ݪ‬ȃ͈̠̓͢ͅಸඤळ޿஽̦၄ͦȂ͈̠̓ٝ͢ͅ໘̳͈̥ͥȂΪΠ͂ಸඤळ޿Ȃພࡔ
ఘ͈ 3 ৪‫ͬ߸۾͈ۼ‬ٜଢ଼̱̞̀ͥȃːȅ૧ܰພࡔఘ࠿੄༹͈‫ٳ‬อ̯ͣ̈́ͥࣞ࢘ၚ̥̾࿌္എ̈́૧ܰພࡔఘ࠿੄༹͈‫ٳ‬อͬ࿒ঐ̱ȂພࡔఘΊΦθͬ௩໙༹̳༷ͥ͞γΑΠΊΦθ͈ੰ‫ٳ༹ͬ̓̈́ݲ‬อ̱̞̀ͥȃ

DNA/RNA ಒ੄ষଲయΏȜΉϋ΍Ȝ଎ˍȇষଲయΏȜΉϋ΍Ȝͥۜ͢ͅஅછ͈ιΗΊΦηΛ·૷౯

ĵı

ΟȜΗٜଢ଼

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଎ˎȇ൚໦࿤ͅ൵ව̯̹ͦষଲయΏȜΉϋ΍Ȝȇ
Roche 454 GS Junior Bench Top System, illumina MiSeq Personal Sequencer, Pacific Biosciences RS System଎ˏȇ;ͼσΑۜஅ႕́࠿੄̯̹ͦ୆໤ਅ͈໦ື
଎ːȇRAPID ͈ດ੔ίυΠ΋Ȝσड߃͈యນഎ̈́ა໲
1. Yasugi M, Nakamura S, Daidoji T, Kawashita N, Ramadhany R, Yang CS, Yasunaga T, Iida T, Horii T, Ikuta K,
Takahashi K, Nakaya T. Frequency of D222G and Q223R Hemagglutinin Mutants of Pandemic (H1N1) 2009
Influenza Virus in Japan between 2009 and 2010. PLoS One. 2012;7(2):e30946.
2. Monira S, Nakamura S, Gotoh K, Izutsu K, Watanabe H, Alam NH, Endtz HP, Cravioto A, Ali SI, Nakaya T, Horii
T, Iida T, Alam M. Gut microbiota of healthy and malnourished children in bangladesh. Front Microbiol.
2011;2:228.
3. Nakamura S, Nakaya T, Iida T. Metagenomic analysis of bacterial infections by means of high-throughput DNA
sequencing. Exp Biol Med (Maywood). 2011;236(8):968-71.
4. Nakamura S, Yang CS, Sakon N, Ueda M, Tougan T, Yamashita A, Goto N, Takahashi K, Yasunaga T, Ikuta K,
Mizutani T, Okamoto Y, Tagami M, Morita R, Maeda N, Kawai J, Hayashizaki Y, Nagai Y, Horii T, Iida T, Nakaya
T. Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput
sequencing approach. PLoS One. 2009;4(1):e4219.
5. Nakamura S, Maeda N, Miron IM, Yoh M, Izutsu K, Kataoka C, Honda T, Yasunaga T, Nakaya T, Kawai J,
Hayashizaki Y, Horii T, Iida T. Metagenomic diagnosis of bacterial infections. Emerg Infect Dis. 2008 Nov;14(11):1784-6.
ĵĲࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȟࣞພࡔ଻ۜஅછࡄ‫ݪ‬໐࿝ႉ઄ۜஅછ‫ݪࡄڠ‬ΈσȜίࡄ‫ݪ‬ΈσȜίઉ̞͒‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁȁȁఱ୞ȁგඃඅහ࣒঍ȁȁ֓‫ڠ‬ฎআȁȁȁྶനȁࢨࢤඅහࡄ‫֥ݪ‬ȁ୆໤ࢥ‫ڠ‬ฎআȁಎ५ȁోढඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁśũŦůźŶġőŪŢŰ
අහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁಅඤȁ౭ࡄ‫ݪ‬Ȇ‫ڰ‬൲ඤယȁ൚ࡄ‫ݪ‬ΈσȜί͉́Ȃˍȫ૟ਥ଻෹‫ۜ޿ݩ׶‬அછȂ෹‫޿ݩ׶‬෹‫૷͈׶‬౯Ȇພఠٜଢ଼͂ထཡȂˎȫήΗႲङ‫ۜ޿ݩ‬அછ͈‫ڠע‬Ȇພఠࡄ‫ݪ‬ȂˏȫΟϋΈ;ͼσΑۜஅછ̤̫ͥࠬͅ઀โࡘઁ‫ܥ‬੬Ȃːȫພࡔळ޿͈ΗϋΩ·ৗ໦๥ι΃ΣΒθͅ‫ݪࡄ̳ͥ۾‬Ȃͬ৽ါ̈́ࡄ‫ݪ‬ΞȜζ̱̞̳͂̀͘ȃ̹͘Ȃ൚ࡄ‫ݪ‬ΈσȜί͉ଲ‫ٮ‬༗࠲‫( ۾ܥ‬WHO)/ Global
outbreak alert & response network (GOARN) ͈ιϋΨȜ̱͂̀ഴ჏̱̤̀ͤȂ૧‫ޟ‬Ȇठ‫ۜޟ‬அછͺ;Πήτͼ·చॐ࣓ͅࡃ̱̳͘ȃˍȫ૟ਥ଻෹‫ۜ޿ݩ׶‬அછȂ෹‫޿ݩ׶‬෹‫૷͈׶‬౯Ȃພఠٜଢ଼͂ထཡ
ˍȅ઀঱Ȃ଼૽̤̫ͥͅ૟ਥ଻෹‫ۜ޿ݩ׶‬அછȂ෹‫଻޿ݩ׶‬෹‫׶‬ȁِș͉̭ͦ́͘ͅͼϋέσ΀ϋΎχ·Ιϋ೰‫ܢ‬୪ਅ‫͈ࣞئ‬Ⴂ৪̤̫ͥͅ 23 ‫ث‬෹‫޿ݩ׶‬χ·Ιϋ͈෹‫׶‬ထཡ࢘‫ض‬Ȃ෹‫֓׶‬ၷ๯ॉࡘ࢘‫̹̱̥ͣͅྶͬض‬ȪVaccine 2010ȫȃ̹͘Ȃ࣭ඤ́อ୆̱̹઀঱૟ਥ଻෹‫ۜ޿ݩ׶‬அછȪIPDȫۛ৪͈ۜஅࠬୄ߿ͅచ̳ͥࠬୄಎ͈ࠬୄ߿අ։ IgG ࢯఘෛഽ͂΂ίΕΣϋ‫଻ڰ‬ȪOPAȫ͈௶೰ͬஜ̧࢜ࡄ‫̱̀͂ݪ‬৘ঔ̱̞̀ͥȃඅͅȂ࣭ඤ͈ 7 ‫ث‬΋ϋΐνΊȜΠχ·Ιϋ୪ਅஜȂ୪ਅࢃ͈઀঱ IPD છ႕͈ພఠٜଢ଼ͬ঑‫̞̱ͥ̀׳‬ȃ̹͘Ȃ଼૽̤̫ͥͅ IPDȂ෹‫଻޿ݩ׶‬෹‫̫̤ͥͅ׶‬ႉ઄࠿ఘ̥͈ͣ PCR ༹ͥࠬ͢ͅୄ߿ࠨ೰Ȃ෹‫޿ݩ׶‬අ։ࢯఘ͈ۜஅཡࢄᲷ౵ࠨ೰ͅ‫̳ͥ۾‬ఉঔ୭ஜ̧࢜ࡄ‫ͬݪ‬৘ঔ̱̞̀ͥȃˎȅPneumococcal surface protein AȪPspAȫχ·Ιϋ͈‫ٳ‬อȁPspA ͉̳͓͈̀෹‫޿ݩ׶‬ນ௄ͅంह̳ͥ΋ςϋࣣࠫ౩ฒৗ́Ȃِș͉̭ͦ́͘ͅ PspA ࠐ๽ු྄χ·Ιϋ୪ਅ̦ͼϋέσ΀ϋΎ;ͼσΑۜஅࢃ͈ඵষ଻෹‫׶‬κΟσ̤̫ͥͅခ࢘଻ͬྶ̥̱̹ͣͅ (Vaccine 2011)ȃ࡛हȂِș͉ͤ͢ࢩ֖̈́ PspA χ·Ιϋ͈‫ٳ‬อͬ࿒ঐ̱̞̀ͥȃˎȫΗͼ́ၠ࣐̳ͥήΗႲङ‫ۜ޿ݩ‬அછ͈ࡄ‫ݪ‬ȁήΗႲङ‫( ޿ݩ‬Streptococcus suis) ͉૽਷‫ވ‬೒ۜஅ޿̜́ͤȂଡ྄‫͈̓̈́׶‬૟ਥ଻ۜஅછͬৼ̳ܳͥȃِș͉̭ͦ
́͘ͅ 2006-8 ා͈Ηͼ࣭ඤ͈૟ਥ଻ۜஅછ 179 છ႕͈ࢃ̧ͧ࢜‫಺ڠע‬औ̥ͣȂΪΠછ႕͉ཤΗͼͅఉ̩อ୆̱Ȃࡔ֦޿͉́ఱ฼̦ࠬୄ߿ˎ (92.2%)Ȃࠬୄ߿ 14(6.7%) ̜̭̦́ͥ͂฻ྶ̱̹ȃ۴ࣁ་ۛ৪̭ͦ́͘ͅ༭͈̥࣬̈́̽
̹ࠬୄ߿ 5Ȅ24 ͥ͢ͅછ႕͜࠿੄̯̹ͦ (Lancet, 2011)ȃࠬୄ߿ 2 ͥ͢ͅ 158 ႕͈ພ߿ٜଢ଼͉́ 58.9% ̦ଡ྄‫́׶‬Ȃॼ̦ͤ෴ࠬછ̜̹̈́̓́̽ȃ৽ါ̈́ STˍ‫͂ڼ‬ȁST104 ‫̴̞ͦ͜ڼ‬෴ࠬછȂଡ྄‫̦̳̭ܳͬ׶‬ȂST1 ‫̦͙͈ڼ‬ອ้ͅଡ྄‫( ̹̥̦̭̳̭̽ͩ͂ܳͬ׶‬Emerg Infect Dis,2011)Ȫ଎ˍȫȃ̹͘ȂཤΗͼȂΩμ΂ࡇ̤̫ͥͅ 2010 ාུ͈છ͈ஜ̧࢜‫಺ڠע‬औ̤̞̀ͅ 31 છ႕̦࠿੄̯ͦȂ̷͈౿ྵၚ͉ 16.1% ̜̹́̽ȃΩμ΂ࡇ๊͈֚ਯྦྷ̜̹͈ͤᑋ
ۛၚ͉Ȃ6.2/100,000/ ා͂ॳ੄̯̹ͦȃ22 ႕Ȫ71%ȫͅ୆ගၳၑ͈୫૙Ⴄ̦̜ͤȂ୫૙ࢃ 2 ඾́૟ਥ଻ۜஅછͬอ
છ̱̞̹̀ (PLoS ONE,2012)ȃ࡛हȂِș͉Ηͼ༗࠲જ͂‫ފ‬ႁ̱Ȃ୆ගၳၑ୯૙ͅచ̳ͥಕփ‫̭̳́͂ͥͬܳۓ‬ཤΗͼུ̥͈ͣછ͈ෳੰͬ࿒ঐ̱̞̀ͥȃˏȫΟϋΈ;ͼσΑۜஅછ̤̫ͥࠬͅ઀โࡘઁ‫ܥ‬੬ȁΟϋΈ͉෎ఝ౷̤̫ͥͅਹါ̈́࢖ਤ‫מ‬୆ષ͈࿚ఴ̜́ͤȂ̷͈ࠬ઀โࡘઁ͈‫ܥ‬੬͉ྚ̺ະྶ̜́ͥȃέͻςάϋ
̤̫ͥͅႉ઄ࡄ‫̥ͣݪ‬Ȃِș͉̭ͦ́͘ͅ exo vivo ͈ࠏ́ۛ৪ဇြ͈ࠬ઀โ͈ζ·υέ͹Ȝΐͥ͢ͅ᭻૙·ςͺρϋΑᄤૺ̦ࠬ઀โࡘઁ͂ခփͅ௖‫( ̹̱̥ͣͅྶ̭̳ͬ͂ͥ۾‬Am J Trop Med Hyg, 2009)ȃࣽٝȂِș͉ྎઑࠬͺεΠȜΏΑࠬ઀โ̦୆ఘඤ͉́έ΁Αέ͹ΙΐσΓςϋ෇ে‫ͬࢹܥ‬̱ٚ̀ζ·υέ͹Ȝΐͤ͢ͅ᭻૙·ςͺρϋΑ̯̭ͦͥ͂ͬྶ̥̱̹ͣͅȪJ Infect Dis,2012ȫȃ‫ ͈ܢ଻ݢ‬thrombopoietin (TPO) ͥࠬ͢ͅ઀โॲ୆̦̭͈ͺεΠȜΏΑࠬ઀โ͈᭻૙·ςͺρϋΑͬ淩̱ٙȂࠬ઀โତ͉ୃુ‫̢̹ͦͣࣉ̳͂ͥا‬ȃĵĳːȫພࡔळ޿͈ΗϋΩ·ৗ໦๥ι΃ΣΒθͅ‫ݪࡄ̳ͥ۾‬ȁळ޿ۜஅ଼͈ၛ͉ͅȂພࡔळ޿͈ॲ୆̳ͥਅș͈ພࡔ֦ঊ̦ఱ̧̈́࿨‫ͬڬ‬౜̞̦̽̀ͥȂ̷ͦͣພࡔ֦ঊ͈ఉ̩
͉৽ͅळ޿̥ͣ໦๥̯ͦͥΗϋΩ·ৗ̜́ͥȃ̷͈̹͛ພࡔळ޿ΗϋΩ·ৗ໦๥‫͞ࢹܥ‬໦๥ΗϋΩ·ৗ͈‫ܥ‬ෝٜͬ

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ଢ଼̳̭͉ͥ͂ພࡔळ޿͈ۜஅι΃ΣΒθͬၑٜ̳ͥષ́ਹါ̜́ͥȃ൚ࡄ‫ݪ‬৒͉́૙ಎඉࡔ֦޿̜́ͥಸ‫׶‬Ϋής΂͞ࡤ‫ۜܕݟ‬அછࡔ֦޿̜́ͥ෹‫͈޿ݩ׶‬঵̾ΗϋΩ·ৗ໦๥௡౾̷ͦͣ̽̀͢͞ͅ໦๥̯ͦͥພࡔ֦ঊ͈‫ܥ‬ෝ̞̾̀ͅࡄ‫̤̭̤ͤ̀̽̈́ͬݪ‬Ȃ࡛हȂ̷ͦͣΗϋΩ·ৗ໦๥ͅຈଌ͈૧ܰΏλβυϋ͈൳೰࣐̞ͬ (FEMS
Microbiol Lett, 2011)Ȃ̹͘໦๥௡౾ͅັଟ̳ͥ ATPase ͈ΗϋΩ·ৗ໦๥͈͒‫۾‬ဓͬ࠿൦̱̞̀ͥȃ଎ˍȇΗͼ̤̫ͥͅήΗႲङ‫ۜ޿ݩ‬அછ͈໦ື͂ພఠ
(Emerg Infect Dis 2011)଎ˎȇΟϋΈ̤̫ͥࠬͅ઀โࡘઁ͂ͺεΠȜΏΑࠬ઀โ·ςͺρϋΑ (J Infect Dis, 2012)ड߃͈యນഎ̈́ა໲
1. Alonzo MTG, Lacuesta TLV, Dimaano EM, Kurosu T, Suarez LC, Mapua CA, Akeda Y, Matias RR, Kuter DJ,
Nagata S, Natividad FF, Oishi K. Platelet apoptosis and apoptotic platelet clearance by macrophages in secondary
dengue virus Infections. J Infect Dis 2012 Apr;205(8):1321-9.
2. Takeuchi D, Kerdsin A, Pienpringam A, Loetthong P, Samerchea S, Pakkinee Loetthong P, Khamisra K, Wongwan
N, Areeratana P, Chiranairadul P, Lertchayanti S, Petcharat S, Yowang A, Chaiwongsaen P, Nakayama T, Yukihiro
Akeda Y, Hamada S, Sawanpanyalert P, Dejsirilert S, Oishi K. Population-based Study of Streptococcus suis
Infection in Humans in Phayao Province in Northern Thailand. PLoS ONE. 2012; 7(2):e31265.
3. Kerdsin A, Dejsirilert S, Sawanpanyalert P, Boonnark A, Noithachang W, Sriyakun D, Simkum S, Chokngam S,
Gottschalk M, Akeda Y, Oishi K. Sepsis and spontaneous bacterial peritonitis in Thailand. Lancet 2011 Sep 3; 378(9794):960.
4. Kerdsin A, Dejsirilert S, Puangpatra P, Sripakdee S, Chumla K, Boonkerd N, Polwichai P, Tanimura S, Takeuchi D,
Nakayama T, Nakamura S, Akeda Y, Gottschalk M, Sawanpanyalert P, Oishi K. Genotypic profile of Streptococcus
suis serotype 2 and clinical features of infection in humans, Thailand. Emerg Infect Dis. 2011 May 17(5):835-42.
5. Akeda, Y, Kodama T, Saito K, Iida T, Oishi, K, Honda T. Identification of the Vibrio parahaemolyticus Type III
secretion system 2-associated chaperone VocC for the T3SS2-specific effector VopC. FEMS Microbiol Lett 2011
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ĵĴࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȟࣞພࡔ଻ۜஅછࡄ‫ݪ‬໐࿝ۜஅळ༾୆໤‫ݪࡄڠ‬ΈσȜίࡄ‫ݪ‬ΈσȜίඅහ‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁȁȁȁȁȁȁ൥‫ז‬ȁဇ‫ج‬ঊඅහ੩‫ޗ‬ȁΨͼ΂΍ͼ΀ϋΑฎআȁଫࡔȁရඅහ੩‫ޗ‬ȁ֓‫ڠ‬ฎআȁȁȁȁȁȁȁઐఆȁఽఱඅහࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁ࿳౓ȁٗࢩࡄ‫ݪ‬ඤယȇ൚ࡄ‫ݪ‬ΈσȜί͉́ळ޿ඉள͂਽৽ळ༾͈௖ࡽैဥͬࡄ‫ݪ‬చય̱̞͂̀ͥȃఉ̩͈ळ޿ඉள͉๷ၾ́਽
৽ͅ౿ঘ̈́̓ఱ̧̈́‫ͬޣג‬ဓ̢̞̠ͥ͂අಭͬ঵̾ȃळ޿ඉள̦̭͈̠̈́͢‫ޑ‬ႁ̈́ैဥͬอ‫̧ͥ́ܞ‬ၑဇ͈֚̾͂
๊̱֚̀എͅࣉ̢̞͈͉ͣͦ̀ͥȂఉ̩͈ळ޿ඉள̦๷ၾ́͜਽৽͈‫ܥ‬ෝ໦ঊͅඅ։എͅैဥ̳ͥࣖள̜̞́ͥ͂
̠̭̜͂́ͥȃ̠֚̾͜ਹါ̈́ळ޿ඉள͈අৗ̱͂̀Ȃैဥ̳ͥ‫ܖ‬ৗ࢘ͅၚ̩͢ΗȜΊΞͻϋΈ̳ͥ‫̻̳ͩ̈́ࢹܥ‬ࢦྥ̈́࿶௣‫̾ͬ͜ࢹܥ‬ાࣣ̦ఉ̞̭̦͂‫̬ͥͦͣݷ‬ȃ̷͈࿶௣‫͉ࢹܥ‬Ȃ͂͂͜͜ळ༾̦‫ུܖ‬എȆ୆ၑഎ̞̽̀͜ͅ
྄ͥ࿶௣ࠏ͞΂σ΄Υρ͈‫ܥ‬ෝ̠̩ͬ͘၌ဥ̱̞̀ͥાࣣ̦ఉ̞ȃਲ̽̀ळ޿ඉள͈࿶௣ࠐႹ͈ࡄ‫͉ݪ‬Ȃඉளͥ͢ͅພఠอ࡛‫͈ࢹܥ‬ٜྶ̞̠͂փ݅ͅ‫̢̀ح‬Ȃਲြ౶̞̥̹ͣͦ̀̈́̽਽৽ळ༾͈‫ུܖ‬എ́ਹါ̱̩͙̈́ͬྶ̥ͣͅ
̧́ͥ‫خ‬ෝ଻͜๩̞͛̀ͥȃ̭͈̠̈́͢ࡄ‫ٸ͉ݪ‬ြພࡔ֦ঊ͈૟වͅచ̳ͥ਽৽͈ཡࢄΏΑΞθͬၑٜ̱ଷࢄ̳ͥ
ષ́͜ਹါ̜́ͥȃ࡛ह൚ࡄ‫ݪ‬ΈσȜί͉́Ȃಸ‫ۯ‬ષ๧ळ༾Ψςͺͬ೒ً̱̀δΜςΤΑ૙ಎඉ̧̭̳ͬ֨ܳδΜςΤΑ૰ࠐඉளໝࣣఘ͈࿶௣ࠐႹ͈ٜྶͬಎ૤̱͂̀ࡄ‫ͬݪ‬ജ‫̞̱ͥ̀ٳ‬ȃδΜςΤΑ૰ࠐඉளໝࣣఘȪB ߿ȫ͈ಸ‫ݟۯ‬ਓ‫͈ࢹܥ‬κΟσ଎‫࣌ۯ‬௰͈δΜςΤΑ૰ࠐඉளໝࣣఘȪB ߿ 16S ඉளȫ͉Ȃӱtranscytosis ͤ͢ͅ‫ܖ‬ೲ྄௰͒֊࣐̳ͥȃӲ‫ܖ‬ೲ྄௰̥ͣ
ໝࣣఘಎ͈ HA ͉ E-cadherin ࣣ̱͂ࠫȂळ༾‫ۼ‬Ψςͺͬ෫ٟ̳ͥȃӳུैဥ̯ͤͣ͢ͅͅఉ̩͈ඉளໝࣣఘ̦ळ༾‫ࠤۼ‬̥ͣఘඤͅ૟ව̳ͥ͂ࣉ̢ͣͦͥȃȁड߃͈యນഎ̈́ა໲
1. Sugawara Y, Fujinaga Y. The botulinum toxin complex meets E-cadherin on the way to its destination. Cell Adh
Migr. 2011; 5(1): 34-36.Ȯ௙୰ȯ
2. Sugawara Y, Matsumura T, Takegahara Y, Jin Y, Tsukasaki Y, Takeichi M, Fujinaga Y. Botulinum HA disrupts theintercellular epithelial barrier by directly binding E-cadherin. J Cell Biol. 2010; 189 (4), 691-700
3. Jin Y1, Takegahara Y1, Sugawara Y, Matsumura T, Fujinaga Y. Disruption of the epithelial barrier by botulinum
hemagglutinin (HA) proteins - Differences in cell tropism and the mechanism of action between HA proteins oftypes A or B, and HA proteins of type C. Microbiology. 2009; 155(Pt 1): 35-45. 1 These authors are contributed
equally.
4. Matsumura T, Jin Y, Kabumoto Y, Takegahara Y, Oguma K, Lencer WI, Fujinaga Y. The HA proteins of botulinum
toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption. Cell Microbiol. 2008; 10(2):355-364.
5. Matsumura T1, Fujinaga Y1, Jin Y, Kabumoto Y, Oguma K. Human milk SIgA binds to botulinum type B 16S toxin
and limits toxin adherence on T84 cells. Biochem Biophys Res Commun. 2007; 352(4), 867-872.
ĵĵࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȟࣞພࡔ଻ۜஅછࡄ‫ݪ‬໐࿝;ͼσΑࡄ‫ݪ‬ΈσȜίࡄ‫ݪ‬ΈσȜίඅහ੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ૩നȁ‫ם‬঄

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ȁίρΑङ RNA ;ͼσΑ͈ఉ̩͉Ȃۜஅࢃ‫ًܢ‬೾ͅ਽৽ळ༾ඤ྄ࠏͬఱܰ࿅ͅठࢹಃ̱Ȃໝୋໝࣣఘ͂ࡤ͊ͦͥ૧ܰ΂σ΄Υρ̩ͬ̾ͥȃ̷͈ࢹ௮ఘ͉઀༾ఘ (ER) ߃ཌྷͅࠁ଼̯ͦȂ̷̭́;ͼσΑΊΦθ͈ໝୋ͂ၥঊࠁ଼̦
࣐̞ͩͦ̀ͥȃ̭͈ໝୋໝࣣఘࠁ଼͉Ȃအș̈́ळ༾ඤΑΠτΑ؊൞̥͈ͣٝ๰ͬ‫خ‬ෝ̱ͅȂಿ‫͈૸ু̹ͤͩͅۼܢ‬֒ഥঊͬໝୋ̵̯̹ͥ͛ͅຈଌ̈́ࢹ௮໤̜́ͥ͂ࣉ̢̞ͣͦ̀ͥȃ൚ࡄ‫ݪ‬ΈσȜί͉Ȃ;ͼσΑ̦ळ༾ৗͅ૧ܰ΂σ΄Υρͬࠁ଼̱ळ༾ඤͅȶ௏૙̠ȷ໦ঊ‫̭̳̥ͬ͂ͥͣͅྶͬࢹܥ‬࿒എ̱͂̀ࡄ‫ͬݪ‬ജ‫̞̱ͥ̀ٳ‬ȃड߃Ȃِș͉ C ߿۴‫ͼ;׶‬σΑ‫͍ݞ‬έρΫ;ͼσΑۜஅळ༾ඤ͈;ͼσΑໝୋໝࣣఘͬୈୋ̱೰ၾίυΞ΂η·Αٜଢ଼࣐̞ͬȂۜஅ̽̀͢ͅඅ։എͅς·σȜΠ̯̩ͦ̀ͥ਽৽֦ঊ߲ͬఉତ൳೰̱̹ȃِș͉Ȃ̭ͦͣ਽৽֦ঊ߲̦Ȃ͈̠̓͢ͅ;ͼσΑໝୋໝࣣఘࠁ଼͈‫ٳ‬ই͂֋঵ͅ‫ܙ‬ဓ̱̞̥̀ͥྶ̥̱ͣͅȂ૧̹̈́਽৽Ƚພࡔఘ͈௖ࡽջం‫ͬ߸۾‬࠿̳̭॑ͥ͂̽̀͢ͅȂ;ͼσΑ͂਽৽ळ༾̦‫ވ‬୆̳̭ͥ͂ͬ‫خ‬ෝ̵̯ͥͅ໦ঊ‫͈ࢹܥ‬ٜྶͬ࿒ঐ̱̞̀ͥȃ̹͘Ȃໝୋໝࣣఘ̷͈͈͈͜ࠁ଼‫̴͙͈ͣ̈́ࢹܥ‬Ȃ;ͼσΑၥঊࠁ଼྄̤̫ͥͅ൲ఠ‫ࢹܥ‬Ȃ;ͼσΑۜஅ̽̀͢ͅည൵̯
ͦͥ਽৽΂ȜΠέ͹ΐȜ‫͈ࢹܥ‬୆ၑ‫ڠ‬എ̈́փ̞ٜ݅̾̀͜ͅଢ଼࣐̞ͬȂඳহ଻ྌ͉૧‫ޟ‬Ȇठ‫ͼ;ޟ‬σΑ଻৖͈ۛଷ
ࢄ༹‫ٳ‬อͅࠒ̦ͥ໦ঊ‫ܖ‬๕͈‫ږ‬ၛͬ࿒ঐ̱̞̀ͥȃ଎ : ;ͼσΑໝୋໝࣣఘȁέρΫ;ͼσΑۜஅ Vero ळ༾͈ࠖ࢕ࡐ๷‫ޢ‬௨ (A-D) ͂ഩঊࡐ๷‫ޢ‬௨ (E,F)ȃ;ͼσΑۜஅ 24 শ‫ࢃۼ‬ͅळ༾ͬࡥ೰̱๱ࢹ௮౩ฒৗࢯఘ (anti-NS2BȂζΔϋΘ )Ȃࢹ௮౩ฒৗࢯఘ (anti-EȂΈςȜϋ ) ࢯఘ̤͍͢ DAPI( Ώͺϋ ) ́அ૗̱̹ȃۜஅळ༾͉́‫̷͈͊ͅړ‬ೄࠂ 5-10mm ͈୆ఘ྄́ս̹ͦ͘;ͼσΑࢯࡔု଻͈ࢹ௮ఘ̦࠿੄̯ͦͥȃໝୋໝࣣఘ͈
ਔ‫׻‬໐͉ͅ઀༾ఘအࢹ௮̦̜ͤȂඤ໐ͅ;ͼσΑအၥঊ͈ಇୟ̦۷ख़̯̹ͦȃड߃͈యນഎ̈́ა໲
1. Morita, E., Sandrin, V., McCullough, J., Katsuyama, A., Hamilton, IB., Sundquist, WI. ESCRT-III protein
requirements for HIV-1 budding. Cell Host Microbe. 2011, 3:235-42.
2. Matsunaga, K., Morita, E., Saitoh, T., Akira, S., Ktistakis, NT., Izumi, T., Noda, T., Yoshimori, T. Autophagy
requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L. J Cell Biol. 2010 190(4):511-21.
3. Morita, E., Colf, LA., Karren, MA., Sandrin, V., Rodesch, CK., Sundquist, WI. Human 5. ESCRT-III and VPS4
proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A. 2010 107(29):12889-94.
4. Bajorek, M., Morita, E., Skalicky, JJ, Morham, SG., Babst, M., Sundquist, WI. Biochemical analyses of human IST1
and its function in cytokinesis. Mol Biol Cell 2009 20(5):1360-73.
5. Kieffer, C., Skalicky JJ., Morita, E., De Domenico, I., Ward, DM., Kaplan, J., Sundquist, WI. A New Functionally
Important ESCRT-III Recognition Mode for the VPS4 ATPases. Dev. Cell 2008, 15(1):62-73.
ĵĶࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȟۜஅଷࢄࡄ‫ݪ‬໐࿝ΊΦθພࡔळ޿‫ݪࡄڠ‬ΈσȜίࡄ‫ݪ‬ΈσȜίඅහ‫ޗ‬਎ȁȁ֓‫ڠ‬ฎআȁ๑നȁഓ࿟අහࡄ‫֥ݪ‬ȁ࿪‫ڠ‬ฎআȁઐനȁਹܵඅහࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁ඾‫݌‬ȁఱܲȁ൚ࡄ‫ݪ‬ΈσȜί͉́ȂΪΠͅພ‫̳̭ܳͬܨ‬ळ޿̞̾̀ΊΦθഎͺίυȜΙͤ͢ͅࡄ‫̞࣐ͥ̀̽ͬݪ‬ȃˍȅພࡔळ޿͈ۜஅȆอພι΃ΣΒθ͈ࡄ‫ݪ‬ȁພࡔळ޿Ȃඅͅজ̹̻̦஠ΊΦθ෻Ⴅͬࠨ೰̱̹ಸ‫׶‬Ϋής΂͈ۜஅȆอພι΃ΣΒθ͈஠ཞͬ໦ঊτασٜ́ྶ̳ͥȃ̷͈षȂພࡔఘ͈̜ͥඅ೰͈ພࡔ֦ঊȪ̢̹͂͊ඉளȫ͈ٜଢ̴଼̺̫͂̓ͣ͘ͅȂζͼ·υͺτͼٜଢ଼͞ͼϋέ΁ζΞͻ·Αͬߐঀ̱ȂΊΦθಎ͈஠֒ഥঊ͈൲̧ͬᅛ១̳̭ͥ͂ͤ͢ͅȂພࡔఘ̦਽৽͂௖ࡽैဥ̳ͥष
͈ພࡔఘ͈֒ഥঊอ࡛͈Θͼ΢ηΒθͬྶ̥̱̞̩ͣ̀ͅȃ̭͈̠̈́͢ͺίυȜΙͤ͢ͅȂࡢș͈֒ഥঊȆ౩ฒ̺̫̭̺͈͉̞ͩͥ́̈́ͅȂ஠ΊΦθૂ༭ͬ‫ڰ‬ဥ̱̹૧̱̞ພࡔळ޿‫ڠ‬Ȇ୆໤‫ͬڠ‬࿒ঐ̳ȃ̹͘Ȃං଼ͣͦͥ‫ܖͅض‬̞̹̿૧ܰ̈́হၷ༹͞ထཡ༹Ȃଇ௸૷౯༹͞۰օ࠿औ༹͈‫ٳ‬อ͜ુͅণ࿤ͅව̞̩ͦ̀ȃ଎ 1. ພࡔळ޿͈ΊΦθٜଢ଼ . ಸ‫׶‬Ϋής΂͈஠ΊΦθ෻Ⴅͬࠨ೰̱̹ȃಸ‫׶‬Ϋής΂͉̲̳ͬ͛͂ͥ
Ϋής΂௺ळ޿͈ΊΦθ͉ˎࡢ͈۪ેஅ૗ఘ̭ͤ̈́ͥ͂ͬ͢ྶ̥̱̹ͣͅȃˎȅ΀ζȜΐϋΈۜஅછ͈੄࡛ι΃ΣΒθ͈ࡄ‫ݪ‬ȁ࡛हȂۜஅછͅ‫̱̀۾‬ଲ‫ٮ‬എͅಕ࿒̯̞ͦ̀ͥ࿚ఴ͈͉͌͂̾΀ζȜΐϋΈۜஅછ͈੄࡛̜́ͥȃ૧̹̈́ۜஅછ
̦੄࡛̱̩̀ͥࡔ֦͉ͅ২ٛഎȆࠐफഎ̈́ါ֦͂͂͜ͅȂພࡔఘ̷͈͈͈͜་‫̢ͥͦͣࣉ̦ا‬ȃ΀ζȜΐϋΈۜஅછ͈੄࡛ι΃ΣΒθ̞̾̀ͅ໦ঊτασٜ́ྶ̯̹ͦ႕͉ພࡔळ޿̤̞͉̺͕̩̀͂ͭ̓̈́͘ͅȂ̭ͦͬྶ̥ͣͅ
̱̞̩̭͉̀͂ࣽࢃ͈૧ܰ̈́΀ζȜΐϋΈۜஅછ͈੄࡛ͅ๵̢̹ͥ͛ͅਹါ̜́ͥȃজ̹̻͉΀ζȜΐϋΈۜஅછ͈੄࡛ι΃ΣΒθٜଢ଼͈౤੣̱͂̀Ȃ߃ාଲ‫ٮ‬എτασ́ၠ̵࣐͙̞ͬ̀ͥಸ‫׶‬Ϋής΂͈૧߿ၠ࣐‫̦ڼ‬ਲြ͈޿‫̥͈̠֑̠͈ͬ̓͂͢ͅڼ‬ΊΦθૂ༭ٜͬ͂͜ͅଢ଼࣐̞ͬ̽̀ͥȃĵķˏȅ୆̧໤̱͈͂̀ພࡔळ޿
ȁພࡔळ޿͈ࡄ‫͉̞̤̀ͅݪ‬Ȃळ޿̦ພ‫̠̞̳̭͂ܳͬܨ‬௰࿂̦අͅಕ࿒̯ͦͥȃ̱̥̱̦͈̈́ͣ͌͂̾୆̧໤͂
̱̀ࡉ̹ાࣣȂ਽৽͈͂௖ࡽैဥ̞͈̾̀ͅ౶ࡉ͞ΊΦθ̞͈̾̀ͅૂ༭̦ࣞഽͅಇୟ̯̞ͦ̀ͥ໦Ȃພࡔळ޿͉

ࡄ
‫ݪ‬
‫ڰ‬
൲
͈
‫ٽ‬
ါ

ྛႁഎ̈́ࡄ‫ऺݪ‬ၳ̢̜̞́ͥ͂ͥȃ̢̹͂͊Ȃಸ‫׶‬Ϋής΂͉ III ߿໦๥௡౾ͬ̾͜ȃIII ߿໦๥௡౾͉ळ޿̦૯‫ړ‬ळ༾͂ྟ୪ͅ௖ࡽैဥ̳̹͈ͬͥ͛ळ޿௰͈௡౾̜́ͥȃ૽ఘ͉ಸ‫׶‬Ϋής΂ུ͈ြ͈ୁ௳۪‫̥̞͉ͣ̈́́ޏ‬Ȃಸ‫׶‬Ϋής΂ུ͉ြ͈ୁ௳۪‫ޏ‬Ȫ٬ဢȫ́ III ߿໦๥௡౾ͬঀ̥͈̽̀̈́ͭͣ૯‫ړ‬୆໤͂ྟ୪̈́௖ࡽैဥ̱̞ͬ̀ͥ
̭̦͂ထே̯ͦͥȃ̭͈̠͢ͅȂ̭̤ͦ́͘͜ͅພࡔ଻ࡄ‫͈ݪ‬۷ത̥ͣಇୟ̧̯̹ͦ̀౶ࡉͬঝ࢛ͅȂພࡔळ޿͈
ুட۪‫̫̤ͥͅޏ‬୆‫̧̞̹̞̱̥̀ͣͅྶ۪ͬڰ‬ȃ଎ˎȅDNA ζͼ·υͺτͼͥ͢ͅພࡔळ޿͈֒ഥঊτΩȜΠς͈ٜଢ଼ȃːȅΊΦθૂ༭ͅ‫̩̿ܖ‬ळ޿ۜஅછ͈ଇ௸૷౯༹͈‫ٳ‬อȁळ޿ۜஅછ͈ଇ௸૷౯༹͈‫ٳ‬อͬ࿒ঐ̱̹ఱܰ࿅‫ܖ؂‬෻Ⴅٜଢ଼ͥ͢ͅພࡔळ޿͈ଇ௸൳೰ΏΑΞθ͈ࢹಃ࣐ͬ̽
̞̀ͥȃड߃͈యນഎ̈́ა໲
1. Hiyoshi H, Kodama T, Saito K, Gotoh K, Matsuda S, Akeda Y, Honda T, Iida T. VopV, an F-actin-binding type III
secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity. Cell Host Microbe. 2011 Oct
20;10(4):401-9.
2. Gotoh K, Kodama T, Hiyoshi H, Izutsu K, Park KS, Dryselius R, Akeda Y, Honda T, Iida T. Bile acid-induced
virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants.
PLoS One. 2010 Oct 13;5(10):e13365.
3. Matsuda S, Kodama T, Okada N, Okayama K, Honda T, Iida T. Association of Vibrio parahaemolyticus
thermostable direct hemolysin with lipid rafts is essential for cytotoxicity but not hemolytic activity. Infect Immun.
2010 Feb;78(2):603-10.
4. Dryselius R, Izutsu K, Honda T, Iida T. Differential replication dynamics for large and small Vibrio chromosomes
affect gene dosage, expression and location. BMC Genomics. 2008 Nov 26;9:559.
5. Nakamura S, Maeda N, Miron IM, Yoh M, Izutsu K, Kataoka C, Honda T, Yasunaga T, Nakaya T, Kawai J,
Hayashizaki Y, Horii T, Iida T. Metagenomic diagnosis of bacterial infections. Emerg Infect Dis. 2008 Nov;14(11):1784-6.
ĵĸࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȟۜஅଷࢄࡄ‫ݪ‬໐࿝ۜஅછ‫ڠ‬Ȇ྾‫ڠע‬ဏࣣࡄ‫ݪ‬ΈσȜίࡄ‫ݪ‬ΈσȜί੎‫ޗ‬਎ġ
ၑ‫ڠ‬ฎআġඅහ࣒঍ ĩ ુ޲ Īġ ฎআ ĩ ၑ‫ ڠ‬Īġ‫֔ז‬ȁࢤਏ
‫ཱݛ‬ȁ౺ঊ

ŋŔőŔ ‫૽࣭ٸ‬අ༆ࡄ‫֥ݪ‬ȁȁȁȁȁȁłůťųŦŦġŎŢųŪŦġŉŶţţŦųĭġőũįŅ
අහࡄ‫֥ݪ‬ȁఱாՏܲঊ

ȁພࡔ޿̦ພ‫̧͉̹̳̭͛ܳ֨ͬͅܨ‬Ȃळ޿̥ͣ਽৽ळ༾͒ȶ෻ోȷ̯ͦͥພࡔ֦ঊ߲͂Ȃ̷͈̹͈͛࿶௣ΏΑΞθ̦ಎ૤എ̈́࿨‫̳̱̹͘ضͬڬ‬ȃজో͉ΪΠͅ෹‫̧̳̭ܳ֨ͬ׶‬τΐ΂Υρ̞̠͂ພࡔ޿ͬκΟσ̱͂̀Ȃ࿶௣ΏΑΞθ̜́ͥ IV ߿໦๥௡౾ (T4SS) ͂Ȃພࡔ֦ঊ̜́ͥ΀έͿ·ΗȜΗϋΩ·ৗ͈൱̧ͬ໦ঊȆࡔঊτασ́ྶ̥̱̠̱̞̳ͣ͂̀͘͢ͅȃȪˍȫIV ߿໦๥௡౾͈ࢹ௮͂‫ܥ‬ෝȁτΐ΂Υρ͉ IV ߿໦๥௡౾ͬ၌ဥ̱̀Ȃτΐ΂Υρ஠ΗϋΩ·ৗ͈֚‫̠̞͂৻ڬ‬ཛྷఱ̈́ତ͈΀έͿ·ΗȜΗϋΩ·ৗͬ਽৽ळ༾ৗ
ಎ͒࿶௣̱̞̳̀͘ȃພࡔ଻ఱಸ޿͞΍σκΥρ̦̈́̓঵̾ III ߿໦๥௡౾͉͂։̈́ͤȂIV ߿໦๥௡౾͈৘ఘ͞໦๥ι΃ΣΒθ͉͕͂ͭ̓ྶ̵̥̯̞ͣͦ̀ͭ͘ͅȃِș͉ IV ߿໦๥͈໦ঊ‫͈ࢹܥ‬ٜྶͬ࿒ঐ̱̀Ȃ໦๥௡౾͈‫ܥ‬ෝȆࢹ௮ٜଢ଼ͬ೒̷̲͈̀৘ఘͅทͤ

଎ 1ȁDotD ͉ఉအ̈́ळ޿໦๥ࠏͅ༗ం̯ͦͥ
ࢹ௮κΙȜέͬ঵̾ȂT4SS ‫྄ٸ‬΋ͺΗϋΩ·ৗ̜̹̞́ͥ͂ࣉ̢̞̳̀͘ȃȪˎȫ΀έͿ·ΗȜ͈‫ܥ‬ෝ̷͈͂ଷࢄ
ȁ̭ͦ́͘ͅ౶̞ͣͦ̀ͥ΀έͿ·ΗȜΗϋΩ·ৗ͈̥͉̈́ͅȂ൳̲਽৽ΗϋΩ·ৗͬΗȜΊΛΠ̳͈͈͂ͥ͜Ȃୃ฽చ͈‫ܥ‬ෝͬ঵̠͈̜̳̾̈́ͤ͘͢͜͜ȃਲ̽̀Ȃ΀έͿ·ΗȜ͈਽৽ळ༾ඤ͈́‫ܥ‬ෝ͉Ȃ୲ྥ̈́শ‫ۼ‬എȆߗ‫ۼ‬എଷࢄͬ਋̫̞̀ͥ͂ࣉ̢̳ͣͦ͘ȃজో͉ఈͅ୶ߐ̫̀τΐ΂Υρ͉́ड੝͈΀έͿ·ΗȜ RalF ͬ൳೰̱̹ఈȂड߃͉́ E3 ξΫ΅Ιϋς΄ȜΔ̱͈͂̀‫ܥ‬ෝͬ঵̾ LubX

଎ 2ȁ΀έͿ·ΗȜͬଷࢄ̳ͥιΗ΀έͿ·
ΗȜ LubX

̦Ȃ৘͉༆͈΀έͿ·ΗȜ͈໅͈শ‫ۼ‬എଷࢄͬংͥȂ̭ͦ́͘ͅ႕
͙̞ͬ̈́Ηͼί͈΀έͿ·ΗȜΗϋΩ·ৗȂιΗ΀έͿ·ΗȜ̧̜̭̞̳́ͥ͂ͬ̾͂͛̀͘ȃड߃͈యນഎ̈́ა໲
1. Tomoko Kubori and Hiroki Nagai. Bacterial effector-involved temporal and spatial regulation by hijack of the hostubiquitin pathway. Front. Microbiol., 2011:2,145.
2. Hiroki Nagai and Tomoko Kubori. Dot/Icm type IVB secretion systems of Legionella and other gram-negative
bacteria. Front. Microbiol.,2011: 2,136.
3. Kubori T, Shinzawa N, Kanuka H, Nagai H. Legionella metaeffector exploits host proteasome to temporally regulate
cognate effector. PLoS Pathog. 2010;6(12),e1001216.
4. Nakano N, Kubori T, Kinoshita M, Imada K, Nagai H. Crystal structure of Legionella DotD: insights into therelationship between type IVB and type II/III secretion systems. PLoS Pathog. 2010;6(10),e1001129.
5. Kubori, T., Hyakutake, A. and Nagai, H. Legionella translocates an E3 ubiquitin ligase that has multiple U-boxes
with distinct functions. Mol. Microbiol. 2008;67(6),1307-1319.
ĵĹࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ‫ڠ‬Ȇ྾‫ڠע‬ဏࣣίυΈρθଔૺ৒ۜஅછ‫ڠ‬Ȇ྾‫ڠע‬ဏࣣίυΈρθଔૺ৒ࡄ‫ݪ‬ΈσȜίࡄ‫ݪ‬ଔૺΈσȜίȁ੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁఆષȁၻঊ‫֗ޗ‬ଔૺΈσȜίȁ੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ൥֔ȁ༠ࣞȁ൚ଔૺ৒͉́Ȃ๷୆໤ພࡄ‫ݪ‬ਫ਼͂྾‫ڠע‬έυϋΞͻͺΓϋΗȜ̷̸̞̠͂ͦͦۜஅછ‫ڠ‬Ȃ྾‫͈ڠע‬ΠΛίτασ
͈ࡄ‫ݪ‬ਫ਼̦໼ၛ̳ͥခ၌۪̈́‫ͬޏ‬डఱࡠͅ୆̥̱Ȃۜஅછ‫ڠ‬Ȃ྾‫͈ڠע‬ဏࣣࡄ‫͈ݪ‬௯ૺॐْܑͬȂ̷ͦͬ৘க̳ͥȃࡄ‫ݪ‬ଔૺުྩȇࡄ‫ݪ‬ଔૺΈσȜί͉́Ȃˍȅུࡄ‫ݪ‬ਫ਼͈৽टྀ́ා˕࠮ͅ‫ٳ‬ट̱̞̀ͥౠႹോۜஅછȆ྾‫ע‬έ΁Ȝρθ ( ࣭ष‫ڠ‬ٛ ) ْܑ͈Ȃ‫א׋‬ȃˎȅུࡄ‫ݪ‬ਫ਼̤̞̀ͅˍώ࠮֚ͅഽ࣐̞ͩͦ̀ͥࡄ‫ݪ‬อນٛȪਬ౴ٛȫْܑ͈Ȃ‫א׋‬ȃˏȅུࡄ‫ݪ‬ਫ਼̤̞̀ͅාˍ࣐̞ٝͩͦ̀ͥఱਬ౴ٛȆުୡอນْܑ͈ٛȂ‫א׋‬ȃːȅέρϋΑΩΑΜȜσࡄ‫ݪ‬ਫ਼Ȇ‫࣭܀‬஠ධఱ‫ڠ‬χ·ΙϋΓϋΗȜȆΗͼۜஅછ‫ވ‬൳࣭षࡄ‫ݪ‬ΓϋΗȜ͈͂࢐ၠমު
ْܑ͈Ȃ‫א׋‬ȃ൝͈‫ڰ‬൲࣐̞ͬ̽̀ͥȃ̭͈ͦͣުྩͬ೒̱̀Ȃ๷୆໤ພࡄ‫ݪ‬ਫ਼ඤ͈ࡄ‫ݪ‬৒‫ފݪࡄ͈ۼ‬ႁȂૂ༭࢐۟Ȃ૽ऺ࢐ၠͬ
௯ૺ̱Ȃࡄ‫ͬޏ۪ݪ‬ା̢Ȃۜஅછ‫ڠ‬Ȇ྾‫̠࣐ͬا଻ڰ͈ڠע‬

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‫֗ޗ‬ଔૺުྩȇ‫֗ޗ‬ଔૺΈσȜί͉́Ȃۜஅછ‫ڠ‬Ȇ྾‫͈ݪࡄڠע‬ဏࣣͬଔૺ̳̹ͥ͛ͅȂˍȅࡄ‫؍شݪ‬౯എ̈́ఱ‫֭ڠ‬໗ίυΈρθ͈ၛ̻ષ̬Ȃ̷͈̹͈͛΃ς΅νρθ͞΋ϋΞϋΜ͈ै଼Ȃ൳໗ίυΈρθ͈‫א׋‬
ˎȅఱ‫֭ڠ‬୆༡ਬ͈ࢩ༭Ȫ୰ྶ͈ٛ‫ٳ‬ट൝ȫȂව‫΂͈ࢃڠ‬ς΀ϋΞȜΏοϋˏȅۜஅછ‫ڠ‬Ȇ྾‫֗ޗڠע‬ίυΈρθȂඅུͅࡄ‫ݪ‬ਫ਼͈඾ུ Į Ηͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜͬဥ̞̹ఱ‫֭ڠ‬୆͈٬‫ٸ‬৘౷ࡄਘ
൝ْܑ͈‫͍ݞ‬৘ঔ൝࣐̠ͬȃȁ̭͈ͦͣުྩͬ೒̱̀Ȃ๷୆໤ພࡄ‫ݪ‬ਫ਼ͬಎ૤̱̹ͅఘࠏഎ̥̾ྛႁഎ̈́ۜஅછ‫ڠ‬Ȇ྾‫؍شݪࡄ͈ڠע‬౯എఱ‫ڠ‬֭‫͈֗ޗ‬იழ͙ै࣐̠ͤͬȃࡄ‫ݪ‬ΈσȜίࡄ‫ݪ‬ΈσȜίȁ੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁఆષȁၻঊȁ྾‫ע‬ະ஠৖ۛࡄ‫ݪ‬໦࿤ͬ࠳හ̱Ȃparoxysmal nocturnal hemoglobinuria (PNH) ΈσȜί͈ςȜΘȜ̱͂̀ո‫ࡄ͈ئ‬‫̞̱ͥ̀ͬݪ‬ȃȪમळ͉ષܱࡄ‫ݪ‬৒͈βȜΐ४ચȫˍȅࢃഛ଻ glycosylphosphatidylinositol (GPI) ࠧఅછȪอै଻࿡‫ࠬۼ‬૗ளෂછ PNHȫ͈อછ‫ܥ‬੬ˎȅ୶ഛ଻ GPI ࠧఅછ͈อછ‫ܥ‬੬ȁˏȅո‫͈ئ‬ζ;Αͬঀ̹̽ ňőŊ ͺϋ΃Ȝ߿౩ฒৗ͈‫ܥ‬ෝഎփ͈ٜ݅ྶ
ȁȁডৗ͈ςκΟςϋΈ͈વ‫ ́ٺ‬ňőŊ ͺϋ΃Ȝ߿౩ฒৗ̦ळ༾྄ષ͈ρέΠͅޫह̧̞́̈́་։ζ;Α͈྾‫ע‬ळ༾͈‫ܥ‬ෝٜଢ଼ȃȁȁࠬ‫ݩ‬අ։എ̈́ GPI ࠧఅζ;Α͈྾‫ע‬ळ༾͈‫ܥ‬ෝٜଢ଼ȃड߃͈యນഎ̈́ა໲
1. Murakami Y, Kanzawa N, Saito K, Krawitz PM, Mundlos S, Robinson PN, Karadimitris A, Maeda Y, Kinoshita T.
Mechanism for release of alkaline phosphatase caused by glycosylphosphatidylinositol deficiency in patients withhyperphosphatasia mental retardation syndrome. J Biol Chem. 2012 Feb 24;287(9):6318-25.
2. Murakami Y, Inoue N, Shichishima T, Ohta R, Noji H, Maeda Y, Nishimura J, Kanakura Y, Kinoshita T.
Deregulated expression of HMGA2 is implicated in clonal expansion of PIGA deficient cells in paroxysmal
nocturnal haemoglobinuria. Br J Haematol. 2012 Feb;156(3):383-7.
3. Murakami H, Wang Y, Hasuwa H, Maeda Y, Kinoshita T, Murakami Y. Enhanced response of T lymphocytes from
Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins. Biochem Biophys Res
Commun. 2012 Jan 27;417(4):1235-41.
4. Krawitz PM, Schweiger MR, Rödelsperger C, Marcelis C, Kölsch U, Meisel C, Stephani F, Kinoshita T, Murakami
Y, Bauer S, Isau M, Fischer A, Dahl A, Kerick M, Hecht J, Köhler S, Jäger M, Grünhagen J, de Condor BJ, Doelken
S, Brunner HG, Meinecke P, Passarge E, Thompson MD, Cole DE, Horn D, Roscioli T, Mundlos S, Robinson PN.
Identity-by-descent filtering of exome sequence data identifies PIGV mutations in hyperphosphatasia mental
retardation syndrome. Nat Genet. 2010 Oct;42(10):827-9.
5. Almeida AM*, Murakami Y*, Baker A, Maeda Y, Roberts IA, Kinoshita T, Layton DM, Karadimitris A. Targeted
therapy for inherited GPI deficiency. N Engl J Med. 2007 Apr 19;356(16):1641-7(* equally contributed).
ĵĺࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activitiesۜஅછ‫ڠ‬Ȇ྾‫ڠע‬ဏࣣίυΈρθଔૺ৒ۜஅછ‫ڠ‬྾‫ڠע‬ဏࣣίυΈρθଔૺ৒ȁ൥֔ΈσȜίࡄ‫ݪ‬ΈσȜί੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ൥֔ȁ༠ࣞ੩‫ޗ‬ȁȁၑ‫ڠ‬ฎআȁ൥നȁຮষ֒ഥঊजඅ։എ୆‫ڠا‬എ΀άΐͿΥΞͻ·Αٜଢ଼༹͈‫ٳ‬อ̷͈͂؊ဥ
ȁ΀άΐͿΥΞͻΛ·ଷࢄ͈໦ঊ‫͈ࢹܥ‬ٜଢ଼͉ȂਲြȂ৽֒ͅഥ‫ڠ‬എٜଢ଼͞দࡑ‫ۯ‬ඤ͈́୆‫ڠا‬എٜଢ଼̽̀͢ͅૺ
̧̹͛ͣͦ̀ȃ̱̥̱Ȃ̭̠̱̹਀༹͉́Ȃ໦ঊ‫͈ࢹܥ‬ٜྶ̦ࣾඳ̜̹́̽ͤȂ̜̞͉ͥȂ਱ාౙպ͈ಿ̞শ‫̦ۼ‬ຈါ̜̹́̽ȃ̭̠̱̹ે‫ͬޙ‬་̢̹ͥ͛Ȃඅ೰ΊΦθႀ֖·υζΙϋࢹ௮͈ non-bias ٜଢ଼ͬ࿒ঐ̱Ȃ૧༹༷ܰა
̱͂̀ insertional chromatin immunoprecipitation (iChIP) ༹ͬ‫ٳ‬อ̱̹ȃȁiChIP ༹͉Ȃ(i) ळ޿͈ DNA ࣣࠫ౩ฒ̜́ͥ LexA ͈ࣣࠫ෻ႥͬȂٜଢ଼చયΊΦθႀ֖߃ཌྷͅொව̱̹ळ༾ͬਏၛȂ(ii) ΗΈͬັ̫̹ LexA DNA ࣣࠫΡιͼϋ (LexA DB) ͈ષܱळ༾͈͒อ࡛Ȃ(iii) έ΁σθͺσΟΪΡ́·υΑςϋ·ࢃȂ಼‫إ‬෨ੜၑ̹͉͘ଷࡠࣖளੜၑͤ͢ͅΊΦθ DNA ͬ౯༌‫ا‬Ȃ(iv) ષܱΗΈͬ෇ে̳ͥࢯఘͥ͢ͅ྾‫ע‬ೆࣛͤ͢ͅȂLexA
DB ̦ࣣ̱̹ࠫ DNA- ౩ฒໝࣣఘͬౙၗȂ(v) ·υΑςϋ·̴͉̱ͬȂໝࣣఘಎ͈౩ฒȆDNAȆRNA ͬ൳೰Ȃ̞̠͂਀ਜ਼ͥ͢ͅȪ଎ȫȃiChIP ༹͉Ȃ(I) ٜଢ଼చયΊΦθႀ֖ࣣࠫͅ
̳ͥ໦ঊ̦ȂDNAȂRNAȂ౩ฒৗ൝Ȃ໦ঊਅͅ‫̴ͣͩ۾‬൳೰̧́ͥȂ(II) ೩΋άȜତ͈ΊΦθႀ֖ࣣ̳ࠫͥͅ໦ঊ͈൳
೰̦‫خ‬ෝ̜́ͥȂ̞̹͂̽‫ܡ‬ం༹͈༷͉ͅྫ̞࿹̹ͦඅৗͬ
঵̞̽̀ͥȃ৘षȂ̭ͦ́͘ͅȂiChIP ༹͂ৗၾ໦ଢ଼༹ͬழ
̵͙ࣣͩ̀ȂΊΦθಎ͈ႋ୪ႀ֖͈ȶ̧̱ͤȷ͈࿨‫ͬڬ‬঵̾ͼϋΑτȜΗȜ෻Ⴅࣣ̳ࠫͥͅ౩ฒৗ‫ ͍ݞ‬RNA ͈൳೰଼ͅ
̱̞ࢗ̀ͥȃ଼̭͈‫͉ض‬Ȃ୆ఘඤͅ߃̞ૄ࠯́Ȃ೩΋άȜତ͈අ೰ΊΦθႀ֖͈ࣣ̳ࠫͥ౩ฒৗͬ൳೰ْ̱̹‫ܢ‬എ଼̈́‫ض‬̜́ͥȃiChIP ༹ͬဥ̞̭ͥ͂́Ȃඅ೰ΊΦθႀ֖ࣣ̳ࠫͅ
ͥྚ౶͈໦ঊȪ౩ฒৗȂDNAȂRNAȂ̷͈ఈȫ͈࿌္എ൳
೰̦‫خ‬ෝ̜́ͥȃȁ࡛हȂiChIP ༹ͬဥ̞̀Ȃ(a) ςϋΩ‫ݩ‬໦‫ا‬ည൵Ȇ֋঵଎ . ொවഎ·υζΙϋ྾‫ע‬ೆ༹ࣛ(insertional chromatin immunoprecipitation: iChIP)(b) ቔ‫ژ‬਋ယఘ͈อ࡛ଷࢄ(c) ͼϋΑτȜΗȜ‫ܥ‬ෝ(d) DNA ඵਹङ୨౯͈෇ে‫̷͈͍ݞ‬ਘ໘(e) ΀άΐͿΥΞͻΛ·‫ܥ‬੬ͥ͢ͅ‫ဲ܈‬ଷ֒ഥঊ͈อ࡛ဲଷ
̞̹͂̽‫ྙޟ‬૬̩̥̾ਹါ̈́୆ྵ࡛ય͈໦ঊ‫͈ࢹܥ‬ٜྶͬ࿒ঐ̱̀ࡄ‫̞ͥ̀͛ૺͬݪ‬ȃड߃͈యນഎ̈́ა໲
1. Fujita T, Fujii H. Direct identification of insulator components by insertional chromatin immunoprecipitation. PLoS
One. 2011;6(10):e26109.
2. Hoshino A, Fujii H. Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions. J
Biosci Bioeng. 2009 Nov;108(5):446-9.
3. Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H. Regulation of Fas-mediated immune homeostasis by anactivation-induced protein, Cyclon. Blood. 2009 Aug 13;114(7):1355-65.
4. Singh AP, Buscaglia CA, Wang Q, Levay A, Nussenzweig DR, Walker J, Winzeler EA, Fujii H, Fonoura BMA,
Nussenzweig, V. Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite.
Cell. 2007 Nov 2;131(3):492-504.
5. Hoshino A, Hirst JA, Fujii H. Regulation of cell proliferation by interleukin-3-induced nuclear translocation of
pyruvate kinase. J Biol Chem. 2007 Jun 15;282(24):17706-11.
Ķı٬‫ݶݪࡄٸ‬തȁResearch Collaboration Center in Overseas
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໲໐‫ڠش‬જġȽۜஅછࡄ‫࣭ݪ‬षΥΛΠχȜ·ଔૺίυΈρθȽఱिఱ‫ۜڠ‬அછ࣭षࡄ‫ݶݪ‬ത඾ུȆΗͼۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜΓϋΗȜಿඅහ‫ޗ‬਎ȁ໌നȁೄგȁۜஅછ͉Ȃχ·Ιϋ͈‫ٳ‬อ͞ࢯ୆໤ৗ͈̈́̓‫ڠا‬ၷ༹͈อోͤ࣪͢ͅ໚̧̹́͂ࣉ̢̹ͣͦশ‫̹̜̦̽ܢ‬ȃ̱̥̱Ȃ߃ා૧̹ͅ੄࡛̱̹အș̈́ۜஅછ ( ૧‫ۜޟ‬அછ ) ͞Ȃ‫࣪ͅܡ‬໚̱̹͂ࣉ̢̞̹͈͈ͣͦ̀͜ठြ ( ठ‫ۜޟ‬அછ )ͬ௖ষ̞́ࠐࡑ̱Ȃۜஅછͅచ̳ͥ২ٛഎະհ̦ଲ‫ٮ‬എ̧̭̹ܳ̽̀ͅȃ̹͘Ȃ̦࣭͉ͩ́ۜஅછࡄ‫ݪ‬ႀ֖͈ࡄ‫ݪ‬৪ະ௷͂͜௖̽̀͘Ȃ֚௄‫̧̞̦ͥ̀̀̽ࣞۜ͘ܥܓ‬ȃ̯ͣͅఉ̩͈ۜஅછ͉࣭‫ͬޏ‬ယօͅ‫̢ק‬Ȅ‫ڐͅ௸ݢ‬ఱ̳ͥম͜ೇ̱̩̩̈́Ȃ࣭֚ౙඊ̷͉͈́૟ව͈ထཡ͞ଷࢄ͉ࣾඳ̜̭́ͥ͂͜ྶ̥̜ͣ́ͥȃ

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œńńĮņœŊ ̥ͣ༗࠲જ΅λϋΩΑͬབ͚ȃֲ௰ͅ ŏŊŉ ͈࠺໤̦ࡉ̢ͥȃȁ̭͈အ̈́෸ࠊ͈‫ئ‬Ȃ໹଼ 17 ාȪ2005 ාȫഽͅอ௷̱̹໲໐‫ڠش‬જ͈ȶ૧‫ޟ‬Ȇठ‫ۜޟ‬அછࡄ‫ݶݪ‬തࠁ଼ίυΈρθȷ̤̫ͥͅ٬‫ݶݪࡄٸ‬ത͈̱֚̾͂̀Ȃఱिఱ‫͉ڠ‬Ηͼ࣭ؐ༗࠲જ֓‫ފ͈ޫڠش‬ႁͤ͢ͅ඾ུȆΗͼ૧‫ޟ‬Ȇठ‫ۜޟ‬அછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜȪRCC-ERI: Research Collaboration Center on Emerging and Re-emerging Infectionsȫȷͬ୭౾̱̹ȃུίυΈρθ͉໹଼ 22 ාȪ2010 ාȫ̥ͣۜஅછࡄ‫࣭ݪ‬षΥΛΠχȜ·ଔૺίυΈρθȪJapan Initiative for Global
Research Network on Infectious Diseases ƣJ-GRIDƤ) ͒͂อജഎ̧֨ͅࠑ̦ͦల 2 έͿȜΒ̦ૺ࣐ಎ̜́ͥȃȁΨϋ΋·߃ࣕΦϋΗής̜ͥͅ༗࠲જȆ֓‫ޫڠش‬ȆΗͼ࣭ၛထཡ‫מ‬୆ࡄ‫ݪ‬ਫ਼ȪNIHȫඤ͈ RCC-ERI ͉ͅȂ࿩
600m2 ͈έυͺͅ P2ȆP3 τασ͈Ψͼ΂ΧΎȜΡచॐͬঔ̱̹৘ࡑ৒ͅ‫̢ح‬Ȃ‫ڎ‬ਅ৘ࡑ‫̦ܕܥ‬୭౾̯̞ͦ̀ͥȃ
RCC-ERI ͉́ NIH ͈ࡄ‫ݪ‬৪͂ྟ୪ͅႲࠈ̱Ȃ૧‫ޟ‬Ȇठ‫ۜޟ‬அછଷգͬ࿒ঐ̱̹ࡄ‫ͬݪ‬ജ‫ͅރ̳͂ͥٳ‬Ȃ̦࣭̤͍ͩ͢Ηͼ͈৹਀ۜஅછࡄ‫ݪ‬৪଼͈֗৾ͤͅழ̞ͭ́ͥȃRCC-ERI ͉ळ޿ۜஅછȂ;ͼσΑۜஅછ͈ 2 ໐࿝ఘଷ͢
ͤ̈́ͤȂ૧‫ޟ‬Ȇठ‫ۜޟ‬அછ͈੄࡛শ͉ͅཡ‫ע‬ષຈါ̈́ૂ༭͈อ૞Ȃহၷ࿪͞χ·Ιϋ‫ٳ‬อ͈̯̰̈́̓̈́͘͘చॐ̦
ଇ௸̢࣐ͥͅఘଷͬ‫ږ‬ၛ̱̞̀ͥȃȁ̯ͣͅࡄ‫͈ݪ‬ႊͬ߃ႋ͈ J-GRID ‫ݶ‬ത࣭͞ඤ‫ڎ‬ఱ‫͈ۜ۾ܥݪࡄ͞ڠ‬அછȟ๷୆໤‫ݪࡄڠ‬৪͜ͅࢩ̬͓̩ͥȂࡄ‫ݪ‬৪΋ϋΕȜΏͺθ͈ࠁ଼ͬ଎ͤȄఉ̩͈ఱ‫ݪࡄ͈ڠ‬৪͈ॷ൳ͬං̞̀ͥȃRCC-ERI ͉ΈυȜΨσͅഥ෥̳ͥۜஅ
છ͈ଷࢄ̫̹࢜ͅ‫ވ‬൳ஜ஌‫ܖ‬౷̱͂̀၌ဥ̱̞̹̺̩̭̦̀͂‫خ‬ෝ̞͂̈́̽̀ͥȃBSL-2 ৘ࡑ৒BSL-3 ৘ࡑ৒ĶĲ٬‫ݶݪࡄٸ‬തȁResearch Collaboration Center in Overseasळ޿ۜஅ໐࿝
ࡄ‫ݪ‬ΈσȜίඅහ‫ޗ‬਎ȁভ‫ڠ‬ฎআġඅහ࣒঍ȁฎআ ĩ ࿪‫ ڠ‬Īġඅහ੩‫ޗ‬ȁ֓‫ڠ‬ฎআġ

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‫ؖ‬നȁგ‫ݛ‬෩ࡍࡄ‫֥ݪ‬ȁၑ‫ڠ‬ਘআȁńũŦŵŴŢťŢġŃŰŰůŵũŪŮŢŵ
෩ࡍࡄ‫֥ݪ‬ȁၑ‫ڠ‬ਘআȁŘŪųŰůŨųŰůŨġŏŢŵŢŬŶŢŵũŶůŨ

ȁΗͼِ࣭̤͍̦࣭ؐͬ͢‫͚܄‬ͺΐͺ੨࣭̱̱́͊͊อ୆̳ͥळ޿ۜஅછͅਹത̤̞ͬ̀Ȃ‫ۜڎ‬அછ͈໦ঊ‫಺ڠע‬औȂ૷౯༹͈‫ٳ‬อȂ֓‫ڠ‬എȆ২ٛഎထཡ༹༷͈‫ٳ‬อ͂৘கͬȂΗͼ༗࠲જ֓‫ޫڠش‬ထཡ‫מ‬୆ࡄ‫ݪ‬ਫ਼͈ࡄ‫ݪ‬৪͂‫ފ‬ႁ̱̾̾Ȃࡄ‫ڰݪ‬൲ͬଔૺ̳ͥȃ
ȁ2010 ȡ 2014 ාഽ̥̫̀ͅ৘ঔಎ͈ȶۜஅછࡄ‫࣭ݪ‬षΥΛΠχȜ·ଔૺίυΈρθȷ̤̞͉̀ͅȄ৽̹ͥࡄ‫ݪ‬చયͬ΋τρ‫͍ݞ‬΋τρ޿̱͖̽̀ͅࡄ‫̞࣐̀̽ͬݪ‬ͥȃΗͼ͉́Ȅ෎ఝ̞̠͂౷ଽ‫ڠ‬എၑဇ̥ͣȄအș̈́ಸ‫ۜۯ‬அછ̦ອอ̱̤̀ͤȄࣽࢃ̷͈͂ͦͣ͜ਹါ଻͉௩̳ম͉̜̽̀͜ࡘͥম͉
̩̈́Ȃࣽࢃ͂͜อജഎͅࠑ௽̳ͥȃȁΗͼ́Ȅ̞ࣞঘཌၚͬা̳ۜஅછ̱͉͂̀
ࡣ̩̥ͣ෹‫׶‬Ȃࠫ‫ړ‬Ȃ‫ئ଻ݢ‬ၓછ൝̦‫̬ͣݷ‬̞ͦ̀ͥȃड߃͉́Ȅਲြພࡔ଻̦৻̞̯͂
̞̹ͦ̀ήΗτϋ΍‫̠͈͢ͅ޿ݩ‬૧̹̈́ພ߿
͂๤‫ڛ‬എ̞ࣞ౿ঘၚͬা̳૧‫ۜޟ‬அછ͈੄࡛
̦ಕ࿒̯̞ͦ̀ͥȃ̭͈ͦͣळ޿ۜஅછͅచ
̳ͥ࿒෻̱ͤͬ̾̾Ȃ̷͈ͦͣထཡ࣓ͅࡃ́
̧ͥࡄ‫ݪ‬ΞȜζͬ୭೰̱Ȃ‫ވ‬൳ࡄ‫͈ݪ‬৘ͬ‫ݷ‬̬̭ͥ͂ͬ࿒ດ̱̞͂̀ͥȃ৽̈́ࡄ‫هݪ‬ఴ˩˨˲˝߿̥ͣࡉ̹Ηͼ࣭ඤ̤̫ͥͅ
΋τρ޿ O1 ͈౷ၑഎ‫ڐ‬८͂ࠐশഎ་‫( ا‬2007 ා ~2010 ා )ˍȫଇ௸૷౯ LAMP ༹ͬߐঀ̱̹΋τρၠ࣐͈ଷࢄȇΗͼȆηλϋζȜ࣭‫̫̤ͥͅޏ‬΋τρࢡอ౷ఝ̤̫ͥͅদ͙
ˎȫ΋τρ޿ၠ࣐‫͈ڼ‬έ͹ȜΐΨς΀ȜΏοϋͅ‫̩̿ܖ‬૧ܰၠ࣐͈࿌္എٜଢ଼ͅ‫ݪࡄ̳ͥ۾‬ˏȫພࡔ଻τϋ΍‫͈޿ݩ‬۰օଇ௸૷౯༹͈‫ٳ‬อ͂؊ဥͅ‫ݪࡄ̳ͥ۾‬ːȫΗͼ̤̫ͥͅພࡔ଻τϋ΍‫ۜ޿ݩ‬அછ͈໦ঊ‫ڠע‬എࡄ‫ݪ‬ड߃͈యນഎ̈́ა໲
1. Okada K, Roobthaisong A, Nakagawa I, Hamada S, Chantaroj S. Genotypic and PFGE/MLVA analyses of Vibrio
cholerae O1: geographical spread and temporal changes of isolates during the 2007-2010 cholera outbreaks in
Thailand. PLoS One. 2012 7(1):e30863.
2. Takeuchi D, Kerdsin A, Pienpringam A, Loetthong P, Samerchea S, Luangsuk P, Khamisara K, Wongwan N,
Areeratana P, Chiranairadul P, Lertchayanti S, Petcharat S, Yowang A, Chaiwongsaen P, Nakayama T, Akeda Y,
Hamada S, Sawanpanyalert P, Dejsirilert S, Oishi K. Population-Based Study of Streptococcus suis Infection in
Humans in Phayao Province in Northern Thailand. PLoS One. 2012 7(2):e31265.
3. Okada K, Chantaroj S, Roobthaisong A, Hamada S, Sawanpanyalert P. A Cholera Outbreak of the Vibrio cholerae
O1 El Tor Variant Carrying Classical CtxB in Northeastern Thailand in 2007. Am J Trop Med Hyg. 2010 82(5):875-878.
4. Puiprom O, Chantaroj S, Gangnonngiw W, Okada K, Honda T, Taniguchi T, Sawanpanyalert P. Identification ofcolonization factors of enterotoxigenic Escherichia coli with PCR-based technique. Epidemiol Infect. 2010 138(4):519-524.
5. Okada K, Chantaroj S, Taniguchi T, Suzuki Y, Roobthaisong A, Puiprom O, Honda T, Sawanpanyalert P. A rapid,
simple, and sensitive loop-mediated isothermal amplification method to detect toxigenic Vibrio cholerae in rectal
swab samples. Diagn Microbiol Infect Dis. 2010 66(2):135-139.
Ķĳ٬‫ݶݪࡄٸ‬തȁResearch Collaboration Center in Overseas;ͼσΑۜஅ໐࿝
ࡄ‫ݪ‬ΈσȜίඅහ‫ޗ‬਎ȁȁ֓‫ڠ‬ฎআȁ໌നȁೄგඅහࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁ‫ئ܅‬ȁජೋঊ෩ࡍࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁŖŢŮűŰųůġŔŪųŪűŢůźŢűũŪůźŰ
෩ࡍࡄ‫֥ݪ‬ȁ֓‫ڠ‬ฎআȁŔŰŮűŰůŨġŔŢűŴŶŵŵũŪűŢŴ
෩ࡍࡄ‫֥ݪ‬ȁၑ‫ڠ‬ฎআȁŏŪŵŤũŢŬŢųůġŏŰųŢůŢŵŦ
෩ࡍࡄ‫֥ݪ‬ȁၑ‫ڠ‬ਘআȁőŪųŢűŰųůġŖŵŢŤũŦŦ
෩ࡍࡄ‫֥ݪ‬ȁၑ‫ڠ‬ਘআȁńũųŪŴġŗŦųŢŵũŢŮūŢŮųŢŴ
෩ࡍࡄ‫֥ݪ‬ȁၑ‫ڠ‬ਘআȁŖųŢůŢůġŕŶŮŬŰŴŪŵ

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ȁ;ͼσΑۜஅ໐࿝͉́ȂΗͼ̤͍̦࣭ͩͬ͢‫͚܄‬ͺΐͺ੨࣭́ۜஅ̦߫ͤ༐̯̞ͦ̀ͥࠬ‫ס‬෾ٚ଻ۜஅછٍ͂෾
ٚ଻ۜஅછͬࡄ‫ݪ‬చયͅ NIH ͈ࡄ‫ݪ‬৪͂‫ވ‬൳ࡄ‫ͬݪ‬ଔૺ̱̞̀ͥȃȁٍ෾ٚ଻ۜஅછ͉́Ηͼͬ‫͚܄‬෎ఝȂՏ෎ఝ౷֖ͅ྘‫̳ͥװ‬Ι·ϋΈϋμͬచય̱͂Ȃࡔ֦;ͼσΑ͈‫ڠע‬എȂ໦ঊ୆໤‫ڠ‬എȂ̤͍͢྾‫ڠע‬എ‫ܖ‬யࡄ‫ͬݪ‬ଔૺ̳ͥȃȁࠬ‫ס‬෾ٚ଻ۜஅછ͉́ HIV ۜஅછ / ΀ͼΒͬచય̱͂ȂΗͼͬই̳͛͂ͥ൐ධͺΐͺ੨࣭ͅ྘‫ ̳ͥװ‬HIV-1
CRF01_AE ‫ͼ;͈ڼ‬σΑ‫ڠ‬എඅಭȂ਽৽‫଻ס‬྾‫ע‬؊൞Ȃ࿪सఛ଻‫ڕ‬ං‫ܖ͈̞̀̾̓̈́ͅࢹܥ‬யഎࡄ‫ͬݪ‬ଔૺ̳ͥȃड߃͈యນഎ̈́ა໲
1. Boonchawalit S, Jullaksorn D, Uttiyoung J, Yowang A, Krathong N, Chautrakul S, Yamashita A, Ikuta K,
Roobsoong A, Kanitvittaya S, Sawanpanyalert P, Kameoka M. Molecular evolution of HIV-1 CRF01_AE Env inThai patients. PLoS One.2011 6: e27098.
2. Utachee P, Nakamura S, Isarangkura-Na-Ayuthaya P, Tokunaga K, Sawanpanyalert P, Ikuta K, Auwanit W,
Kameoka M. Two N-linked glycosylation sites in the V2 and C2 regions of human immunodeficiency virus type 1
CRF01_AE envelope glycoprotein gp120 regulate viral neutralization susceptibility to the human monoclonal
antibody specific for the CD4 binding domain. J Virol. 2010 84: 4311-20.
3. Soonthornsata B, Tian YS, Utachee P, Sapsutthipas S, Isarangkura-na-Ayuthaya P, Auwanit W, Takagi T, Ikuta K,
Sawanpanyalert P, Kawashita N, Kameoka M. Design and evaluation of antiretroviral peptides corresponding to the
C-terminal heptad repeat region (C-HR) of human immunodeficiency virus type 1 envelope glycoprotein gp41.
Virology 2010 405: 157-64.
4. Li YG, Chittaganpitch M, Waicharoen S, Kanai Y, Bai GR, Kameoka M, Takeda N, Ikuta K, Sawanpanyalert P.
Characterization of H5N1 influenza viruses isolated from humans in vitro. Virol J. 2010 7: 112.
5. Kanai Y, Boonsathorn N, Chittaganpitch M, Bai G, Li Y, Kase T, Takahashi K, Okuno Y, Jampangern W, Ikuta K,
Sawanpanyalert P. The impact of antigenic drift of influenza A virus on human herd immunity: Sero-epidemiologicalstudy of H1N1 in healthy Thai population in 2009. Vaccine 2010 28: 5437-44.
ĶĴ٬‫ݶݪࡄٸ‬തȁResearch Collaboration Center in Overseasఱि Į ζΪΡϋۜஅછΓϋΗȜȪŎŐńŊŅȫ
ࡄ‫ݪ‬ΈσȜί‫ޗ‬਎Ȫ࠳ȫġ ਷֓‫ڠ‬ฎআȁઐ‫׆‬ȁ஝হඅහ੎‫ޗ‬਎ġ ਷֓‫ڠ‬ฎআȁ‫ؖ‬ႅȁ۪ਏඅහࡄ‫֥ݪ‬ġ ֓‫ڠ‬ฎআȁȁॕ५ȁ๼‫ܮ‬ঊ෩ࡍࡄ‫֥ݪ‬ġ ֓‫ڠ‬ฎআȁȁőųŰŮŴŪůġŎŢŴųŪůŰŶŭ
෩ࡍࡄ‫֥ݪ‬ġ ၑ‫ڠ‬ਘআȁȁŐųŢűŪŮġőŶŪűųŰŮ
෩ࡍࡄ‫֥ݪ‬ġ ၑ‫ڠ‬ਘআȁȁőŢůūŢűŰųůġńũŢŪŤũŢůŢ

ȁ෎ఝ౷ٍ֖̤̫ͥͅ෾ٚ଻৖͉ۛȂ߃ා͈౷‫أݩ‬౰‫ฺ̞ͅا‬Ȃ̷͈ၠ࣐౷֖ͬ‫ڐ‬ఱ̱̞̀ͥȃΗͼ࣭ؐ́࿚ఴ͂
̞̈́̽̀ͥȂΟϋΈ෎ İ ΟϋΈ੄ࠬ෎͞Ι·ϋΈϋμ෎ٍ͈̈́̓෾ٚ଻͈;ͼσΑ଻৖ۛͅ‫̱̀۾‬Ȃଇ௸૷౯΅ΛΠ͈‫ٳ‬อȂΪΠٍ͍̤̫̈́ͣͥͅͅ;ͼσΑ໦ື಺औȂ̷̱̀อછι΃ΣΒθ͈ٜྶ̫̹࢜ͅࡄ‫̠࣐ͬݪ‬ȃඅͅႉ઄࠿ఘͬဥ̞̹ٜଢ଼ͬζΪΡϋఱ‫ڠ‬෎ఝ֓‫ڠ‬໐͂‫ވ‬൳́ଔૺ̳ͥȃ̭͈ͦͣ‫ވ‬൳ࡄ‫ͬݪ‬೒̱̀ȂζΪΡϋఱ‫ڠ‬෎ఝ֓‫ڠ‬໐ِ̤͍̦࣭͈ۜ͢அછࡄ‫ݪ‬৪଼͈֗͜ͅႁͬಕ̪ȃ৽̈́ࡄ‫هݪ‬ఴ1) Ηͼٍ̤̫̤͍ͥ͢ͅΪΠٍ̤̫ͥͅ෾ٚ଻;ͼσΑ͈૟֪ે‫಺͈ޙ‬औȃ2) ΟϋΈ;ͼσΑ͂Ι·ϋΈϋμ;ͼσᾼచ̳ͥΪΠ̤͍͢ζ;ΑκΦ·υȜ΢σࢯఘ͈ैୋ͂Ȃ̷͈଻ેٜଢ଼ȃ3) ैୋ̱̹κΦ·υȜ΢σࢯఘͬဥ̞̹૷౯΅ΛΠ‫ٳ‬อȃड߃͈యນഎ̈́ა໲
1. Co-existence of major and minor viral populations from two different origins in patients secondarily infected withdengue virus serotype 2 in Bangkok. Puiprom O, Yamashita A, Sasayama M, Limkittikul K, Boonha K,
Jittmitraphap A, Leaungwutiwong P, Kurosu T, Ramasoota P, Ikuta K. Biochem Biopys Res Commun. 2011, 143:
136-142.
2. Fab MAbs specific to HA of influenza virus with H5N1 neutralizing activity selected from immunized chicken
phage library. Pitaksajjakul P, Lekcharoensuk P, Uparagarin N, Barbas C, Ibrahim MS, Ikuta K, and Ramasoota P.
Biochem Biopys Res Commun. 2010, 395: 496-501.
Ķĵ٬‫ݶݪࡄٸ‬തȁResearch Collaboration Center in Overseas
ΟϋΈχ·ΙϋȪिఱ๷୆໤ພࡄ‫ݪ‬ٛȫ‫ݪࡄ້ܙ‬໐࿝ࡄ‫ݪ‬ΈσȜί‫ݪࡄ້ܙ‬໐࿝‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁȁȁ઀ୌȁ‫ם‬ඵ‫ݪࡄ້ܙ‬໐࿝੩‫ޗ‬ȁ༗࠲‫ڠ‬ฎআȁȁ५ಎȁකঃඅහࡄ‫֥ݪ‬ȁȁȁȁ༗࠲‫ڠ‬ฎআȁȁ߬ࡔȁ२გ

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ȁ़๊֚౬༹૽िఱ๷୆໤ພࡄ‫ݪ‬̥ٛͣఱिఱ‫ڠ‬๷୆໤ພࡄ‫ݪ‬ਫ਼͈͒‫້ͤ͢ͅܙ‬Ȃ2011 ාഽ̥ͣΗͼ͈ζΪΡϋఱ‫ڠ‬෎ఝ֓‫ڠ‬໐ඤͅȂΟϋΈχ·ΙϋȪिఱ๷୆໤ພࡄ‫ݪ‬ٛȫ‫ݪࡄ້ܙ‬໐࿝̦อ௷̱̹ȃȁΟϋΈ෎͉Ȃ෎ఝ౷֖ͅࢩ̩ၠ࣐̱Ȃˍ඾ͅ࿩ 30 ྔ૽͈૧̹̈́ۛ৪อ୆̦ଔ೰̯ͦͥडਹါٍ͈෾ٚ଻;ͼσΑ৖̜ۛ́ͥȃਹછ߿͈ΟϋΈ੄ࠬ෎͉Ȃഐ୨̈́হၷ̦࣐̫ͩ̈́ͦ͊౿ྵၚ̦ 20% ͅષઌ̳ͥȃ̱̥̱Ȃ෇‫خ‬χ·Ιϋ͉̩̈́අ։എࢯ;ͼσΑस͜‫ږ‬ၛ̯̞̞ͦ̀̈́ȃχ·Ιϋ͉ड͜ຽ༑എ̈́ထཡ਀౲̜́ͤȂ̷͈‫ٳ‬อ͉‫ݢ‬ྩ͈‫ه‬ఴ̜́ͥȃȁ൚ࡄ‫ݪ‬໐࿝͉́ȂΟϋΈ෎Ȇ੄ࠬ෎͈อછ‫ܥ‬੬ٜͬྶ̳ͥࡄ‫ݪ‬ȂΟϋΈ;ͼσΑ͈ඉ଻Ȃഥ෥͞ૺ‫ࡄ̳ͥ۾ͅا‬‫ͅވ͂ݪ‬Ȃਅș͈୽ၞͥ͢ͅΟϋΈχ·Ιϋ‫ٳ‬อ͈‫ܖ‬யࡄ‫̠࣐ͬݪ‬ȃρδ໓ࠊड߃͈యນഎ̈́ა໲
1. Eiji Konishi, Yuko Miyagawa: Balance of infection-enhancing and neutralizing antibodies induced by a dengue
tetravalent DNA vaccine in a mouse model. Microbes and Infection. 13(12-13):1091-8, 2011
2. Eiji Konishi, Mayu Konishi: Nonstructural Protein 1 Antibody-Based Epitope-Blocking Enzyme-Linked
Immunosorbent Assay to Differentiate Japanese Encephalitis Virus from Dengue Virus Infections in Humans. Jpn J
Infect Dis. 64(4):284-91, 2011
3. Atsushi Yamanaka, Kris C. Mulyatno, Helen Susilowati, Eryk Hendrianto, Amor P. Ginting, Dian D. Sary, Fedik A.
Rantam, Soegeng Soegijanto, Eiji Konishi: Displacement of the Predominant Dengue Virus from Type 2 to Type 1
with a Subsequent Genotype Shift from IV to I in Surabaya, Indonesia 2008-2010. PLoS One. 2011;6(11):e27322.
Epub 2011 Nov 7.
4. Kris C. Mulyatno, Atsushi Yamanaka, Ngadino, Eiji Konishi: Resistence of Aedes aegypti laevae to temephos
insecticide in Surabaya, Indonesia. Southeast Asian J Trop Med Public Health. 43:29-33. (2012).
5. Kris C. Mulyatno, Helen Susilowati, Atsushi Yamanaka, Soegeng Soegijanto, Eiji Konishi: First isolation andphylogeny of Chikungunya virus from Surabaya, Indonesia. Jpn J Infect Dis. 65, 92-94 (2012).
ĶĶΈυȜΨσ COE ίυΈρθȁGlobal COE ProgramΈυȜΨσ COE ίυΈρθ΂σ΄ΥρΥΛΠχȜ·֓‫ڠ‬஻଼ίυΈρθ
ȁ࡛ह͈֓‫ڠ‬Ȃ୆ྵ‫͈ڠش‬ड͜ਹါ̈́‫ه‬ఴ͈͉͌͂̾Ȃ୆ྵͬΏΑΞθ̱͂̀ၑٜ̱Ȃ̷͈ၑٜͅ‫̩̿ܖ‬৖͈ۛထཡ͞૧̹̈́হၷ༹͈̠ͬ̓͢ͅ‫̧̳̭̠̞̥̦̭̩̞̱́͂͂ͥ́͂̀ఽٳ‬ȃ̭͉ͦȂౙ֚֒ഥঊພ͈̠̈́̓͢ͅȂ
1 ͈̾֒ഥঊ་։́ည൵̯ͦͥౙ֦ঊ৖̴͈͙ۛ̈́ͣȂఉ̩͈֒ഥঊ۪͞‫ޏ‬ါ֦̦ໝॠͅ၁͙ࣣ̽̀อછ̳ͥȂ̞ͩͥ͠ఉ֦ঊ৖ۛ͞Ȃພࡔఘ̦ळ༾ඤ͈အș֦̈́ঊ͈͂௖ࡽैဥͬ‫ڰ‬ဥ̱̀਽৽ळ༾͈͒ۜஅ଼ͬၛ̵̯ͥۜஅછ
͈̈́̓Ȃໝॠ̈́৖͈ۛພఠٜྶȂহၷȂထཡ͒૧̹̈́ൽͬఽ̩̹͉͛ͅޭ͛̀ਹါ̈́ಯ୽̜́ͤȂ২ٛഎ͜ͅຈါ
଻͈̞ࣞΞȜζ̳́ȃ̭͈̠̈́͢෇েͅ‫̧̿ܖ‬Ȃུ‫ݶ‬ത͉́Ȃ̭ͦ́͘໦ঊΥΛΠχȜ·͈ၑٜͅၣ̞̽̀ͥ͘୆
ྵ‫ڠش‬Ȇ֓‫ͬݪࡄڠ‬อജ̵̯Ȃळ༾ͬࢹ଼̳ͥ‫ܥ‬ෝ໦ঊਬࣣఘ̱͂̀ड͜ਹါ̈́΂σ΄Υρͅ಍࿒̱Ȃအș̈́΂σ΄Υρ‫ܥ͈ۼ‬ෝഎΥΛΠχȜ·Ȫ΂σ΄ΥρΥΛΠχȜ·ȫ͈ٜྶͬ࿒ঐ̳͂͂͜ͅȂ̯͉ͣͅȂ̷͈ၑٜͅ‫̿ܖ‬̞̀ȂۜஅછȂ૰ࠐ་଻৖͈ۛ̈́̓ພఠུ͈ৗٜͬྶ̱Ȃ૧̹̈́হၷ୽ၞ͈͒ඏ෫࢛ͬఽ̩̭͂Ȫ΂σ΄ΥρΥΛΠχȜ·֓‫͈ڠ‬஻଼ȫͬ࿒എ̱̞̳͂̀͘ȃ̭͈࿒എͬో଼ུ̳̹ͥ͛ͅ‫ݶ‬ത̦ຝ̩ࡄ‫ࢹݪ‬ே͉ո‫͈ئ‬೒̳ͤ́ȃ
1. ΂σ΄ΥρΥΛΠχȜ·ٜྶ͈͒໦࿤ဏࣣഎࡄ‫͈ݪ‬ଔૺȁພࡔఘ̦ळ༾͈΂σ΄ΥρΏΑΞθͬࢦͅ၌ဥ̱̀਽৽ळ༾ۜͅஅ̳̞̠ͥ͂ۜஅછུ͈ఠͅ಍࿒̱Ȃۜஅ‫ࢹܥ‬ͬດഎ̱͂̀ळ༾୆໤‫ڠ‬എণത̥ٜͣଢ଼̳̭ͥ͂Ȃ̹͘Ȃအș̈́΂σ΄Υρ‫ܥ‬ෝͅྟ୪ͅ‫ͥͩ۾‬ൠङ΍ͼ·σͬచય̱ٜ̀ͅଢ଼̳̭ͥ͂ͬ೒̱̀Ȃ΂σ΄ΥρΥΛΠχȜ·ٜྶͬ࿒ঐ̱̳͘ȃ
2. ΂σ΄ΥρΥΛΠχȜ·͈ၑٜͅ‫̩ۜ̿ܖ‬அછȂ૰ࠐ་଻৖͈ۛ̈́̓ພఠ͈ൡࣣഎၑٜ͂হၷ୽ၞ
ȁ̭͈ͦ́͘ণത̥ͣ౎‫̱ݕ‬ȂΏΑΞθΨͼ΂υΐȜ༹͈༷̈́̓აͬୟޭഎ৾ͤͅවͦȂ΂σ΄ΥρΥΛΠχȜ·͈۷ത̥ͣȂۜஅછ͞૰ࠐ་଻৖͈ۛ̈́̓ໝॠ̈́৖ۛͬ૯ͅၑٜ̳̭ͥ͂Ȃ̯ͣͅȂ΂σ΄ΥρΥΛΠχȜ·͈ၑٜͅ‫̧̿ܖ‬Ȃ৖͈ۛພఠུ͈ৗͅ‫ͥͩ۾‬ΗϋΩ·ৗ͞ൠङਘૐͬࡉޭ͛Ȃ̷ͦͣͬດഎ̱̹ͅ૷౯༹͞૧ܰহၷͬ
‫ٳ‬อ̳̭ͥ͂ͬ࿒ঐ̱̳͘ȃ̹͘Ȃࣞഽ̈́ဏࣣࡄ‫ܖݪ‬๕ͬ঵̾‫ݶ‬തͬࠁ଼̳̹͉ͥ͛ͅȂ࣭षഎͅ‫ڰ‬࿬̧́ͥȂ࿹̹ͦ৹਀ࡄ‫ݪ‬৪͈૽ऺ଼̦֗ຈଌ̳́ȃུ‫ݶ‬ത͉́Ȃఱ‫֭ڠ‬୆͞৹਀ࡄ‫ݪ‬৪̦ςȜΘȜΏΛίႁȂ࣭षۜ‫ͬژ‬෽̢ͥ૽ऺ଼֗ίυΈρθͬ৘ঔ̳ͥ͂͂͜ͅȂଲ‫ٮ‬ΠΛίτασࡄ‫ݶݪ‬തȶ྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜȷȂ๷୆໤ພࡄ‫ݪ‬ਫ਼ۜஅછ࣭षࡄ‫ݪ‬ΓϋΗȜȂΗͼ࣭ۜஅછ‫ވ‬൳ࡄ‫ݪ‬ΓϋΗȜͬခ‫ܥ‬എ͍̫࣭ࠫ̾ͥͅषΥΛΠχȜ·‫ݶ‬ത̱͈͂̀࿨‫̹̳̹͛ضͬڬ‬Ȃ٬‫ڠ͈̥ͣٸ‬୆͞৹਀ࡄ‫ݪ‬৪ͬ਋̫වͦȂ࣭ͤ͢षഎ̈́‫֗ޗ‬ΏΑΞθͬࢹಃ̱̞̀
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মުଔૺ౜൚৪
ঙȁྴ࡛ह͈୺࿝Ȇ‫ڠ‬պਫ਼௺໐ޫȪ୺ࢲ൝ȫȆ૖ྴༀ ന ‫߼ ץ‬ळ༾୆໤‫ڠ‬Ȇ֓‫ڠ‬ฎআ୆ྵ‫ܥ‬ෝࡄ‫شݪ‬Ȫ୆ྵ‫ܥ‬ෝ୺ࢲȫȪ࠳ȫ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎࡫ ന ో ঃ֒ഥ֓‫ڠ‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆઉ̞͒‫ޗ‬਎೚ ུ ٖ ‫ם‬֒ഥ֓‫ڠ‬Ȇၑ‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎ಅ ന ȁ ࠩ྾‫ڠע‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎ಱ ࿤ გ ങۜஅછ‫ڠ‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎༌ ५ ణ ֚૰ࠐळ༾‫ڠ‬Ȇ֓‫ڠ‬ฎআႲࣣ઀঱อో‫شݪࡄڠ‬Ȫ઀঱อో‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎ಎ ఆ ຮ ֚୆‫ڠا‬Ȇၑ‫ڠ‬ฎআॲ‫ڠ‬Ⴒࠈུ໐Ȇඅහ‫ޗ‬਎ಅ ࡔ ഔ ჊ඤ‫ڠش‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎२ ஝ ‫ ם‬౶ႉ઄࠿औ‫ڠ‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ༗࠲‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎გ ന ༿ ೄ໦ঊພఠ‫ڠ‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆઉ̞͒‫ޗ‬਎൥ུ̥ͤ͠ခ‫ڠاܥ‬Ȇၑ‫ڠ‬ฎআၑ‫شݪࡄڠ‬Ȫ‫ࢲ୺ڠا‬ȫȆ੎‫ޗ‬਎౓ ࢛ ೄ ෗୆‫ڠا‬Ȇ֓‫ڠ‬ฎআၑ‫ݪࡄڠا‬ਫ਼ȪΏΑΞθൠङ୆໤‫ݪࡄڠ‬ΈσȜίȫȆΈσȜίΟͻτ·ΗȜ‫ ݌‬૩ ȁ ༗ळ༾୆໤‫ڠ‬Ȇ֓‫ڠ‬ฎআ୆ྵ‫ܥ‬ෝࡄ‫شݪ‬Ȫ୆ྵ‫ܥ‬ෝ୺ࢲȫȆ‫ޗ‬਎݊ ౓ ȁ ૾྾‫ڠע‬Ȇ֓‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫ୆ఘཡࢄࡄ‫ݪ‬໐࿝ȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫ࿒‫ح‬ന‫ם‬༟ळ༾୆໤‫ڠ‬Ȇ֓‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫ୆ఘཡࢄࡄ‫ݪ‬໐࿝ȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫઐ ‫ ׆‬஝ হ;ͼσΑ‫ڠ‬Ȇ਷֓‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫۜஅ‫ݪࡄࢹܥ‬໐࿝ȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫ‫ ؂‬ന ో ဎ;ͼσΑ‫ڠ‬Ȇ֓‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫۜஅ‫ݪࡄࢹܥ‬໐࿝ȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫཱ ࢛ հ ຃ळ޿‫ڠ‬Ȇ෠‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫۜஅ‫ݪࡄࢹܥ‬໐࿝ȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫ୆ ന გ ၻ;ͼσΑ‫ڠ‬Ȇ֓‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫཱ ֔ ੇ ࢤ‫ܙ‬୆ಖ‫ڠ‬Ȇၑ‫ڠ‬ฎআ๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫඳহۜஅછచॐࡄ‫ݪ‬ΓϋΗȜȫȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫૣ ၻ ୓ ౳྾‫ڠע‬Ȇ֓‫ڠ‬ฎআ྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫ࿐‫ئ‬Ηυ;྾‫ڠע‬Ȇ֓‫ڠ‬ฎআ྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫ࣏ ଳ ȁ ં྾‫ڠע‬Ȇ֓‫ڠ‬ฎআ྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜȆ‫ޗ‬਎ȁȪ‫ފ‬ႁ࣒जȇ֓‫ࢲ୺ڠ֓شݪࡄࠏڠ‬ȫߧ Φ ‫ ޡ‬੓྾‫ڠע‬Ȇ֓‫ڠ‬ฎআ֓‫شݪࡄࠏڠ‬Ȫ֓‫ࢲ୺ڠ‬ȫȆ‫ޗ‬਎ႝ ࿐ ȁ ‫ދ‬୆‫ڠا‬Ȇၑ‫ڠ‬ฎআၑ‫ݪࡄڠا‬ਫ਼ȪΏΑΞθൠङ୆໤‫ݪࡄڠ‬ΈσȜίȫȆΙȜθςȜΘȜ५ ࢛ ༿ ਏࢹ௮୆໤‫ڠ‬Ȇ࿪‫ڠ‬ฎআၑ‫ݪࡄڠا‬ਫ਼ȪΏΑΞθൠङ୆໤‫ݪࡄڠ‬ΈσȜίȫȆΙȜθςȜΘȜ࿨‫ڬ‬໦౜Ȫ੝ාഽ͈‫ݶ‬തࠁ଼ْ̤̫ࠗͥͅ໦౜মࣜȫ

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‫ݶ‬തࠁ଼͈ൡ‫΂͂گ‬σ΄ΥρΥΛΠχȜ·ٜྶ޼ΐΑΠυέͻȜ͈ൠङਘૐ̤͍͢૰ࠐ་଻৖ۛ
ळ༾ঘ͈‫΂͂ࢹܥ‬σ΄Υρুட྾‫଻ڰ͈ࠏע‬ଷࢄ‫͈ࢹܥ‬ٜଢ଼ࢯ޿‫଻ڰ‬ດഎ͈ٜଢ଼͂૧ܰࢯ޿࿪͈‫ٳ‬อ૰ࠐ‫ܥ‬ෝ։ુ͂΂σ΄Υρ
HGFͥ͢ͅ૰ࠐ་଻৖ۛ͞ଅ৖͈ۛ̈́̓হၷ୽ၞ
۴‫͈׶‬อછ‫͂ࢹܥ‬হၷ୽ၞൠङܿ੅ͬဥ̞̹Ψͼ΂ζȜ΃Ȝ͈‫ٳ‬อ̷͈͂‫ܥ‬ෝٜଢ଼ൠङٜଢ଼༹͈‫ٳ‬อ͂୶ഛ଻ൠङະ஠͈ٜଢ଼
ळ޿ဇြໝࣣൠৗ෇েͥ͢ͅळ޿૟ව࠿౶‫ࢹܥ‬ൠङȆΗϋΩ·ৗ͈‫ܥ‬ෝٜଢ଼ۜஅȆ྾‫̫̤ͥͅע‬ιϋήτϋΠρέͻΛ·͈࿨‫͈ڬ‬ٜଢ଼‫ڕ‬ං྾‫͈ע‬൲ఠͅ‫ݪࡄ̳ͥ۾‬ΐέΞςͺඉள͈ඉ଻อ࡛ͅ‫ͥͩ۾‬ळ༾௰֦ঊ͈ٜଢ଼
C߿۴‫ͼ;׶‬σΑ͈ۜஅ‫̷͈͂ࢹܥ‬ଷࢄ༹ͅ‫ݪࡄ̳ͥ۾‬HIVۜஅછͅ‫ͥͩ۾‬਽৽֦ঊ͈ࡄ‫ݪ‬ळ޿଻ພࡔ֦ঊ͈‫ܥ‬ෝ͂ࢹ௮͈ٜଢ଼
૧‫ͼ;ޟ‬σΑ͈ۜஅ̷͈͂ພఠ‫ܥ‬੬ͅ‫ݪࡄ̳ͥ۾‬ζρςͺχ·Ιϋ͈‫ٳ‬อ͂਽৽Ƚ‫ܙ‬୆ಖ௖ࡽैဥ͈ٜଢ଼
ুட྾‫ݪࡄ͈ע‬਽৽ພࡔఘ௖ࡽैဥ̤̫ͥͅGPIͺϋ΃Ȝ͈փ͈ٜ݅ଢ଼
ພࡔఘͥ͢ͅ྾‫ע‬ଷࢄ‫ݪࡄ͈ࢹܥ‬྾‫಺ע‬୯Ȇळ༾൲ఠଷࢄ໦ঊ͈ࡄ‫ݪ‬ဋၗൠङ̤͍͢ൠΗϋΩ·ৗ͈຦ৗ‫ۯ‬ၑ
NMRͥ͢ͅໝࣣൠৗ͈ࢹ௮ٜଢ଼

Ķĸࡄ‫ڰݪ‬൲͈‫ٽ‬ါȁResearch & Activities୆૓ळ༾ΈσȜίࡄ‫ݪ‬ΈσȜί੎‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ࿤ॄȁୃ๼ˍȅ୆૓ळ༾͈΀άΐͿΥΞͻ·Αȁୈ௏୆૓ळ༾අ։എ֒ഥঊ͈ఉ̩͉τΠυεΖΰȂ֒ഥঊඤ໐ͅ CpG-rich ႀ֖ͬ঵̾ȃ֒ഥঊඤ໐͈ιΙσ‫̦ا‬ఘळ༾͈́อ࡛ͬဲ̢Ȃ୆૓ळ༾͈́౎ιΙσ‫̦ا‬อ࡛ͅຈါ
̜̭́ͥ͂ͬྶ̥̱̹ͣͅȃ࡛हȂΪΑΠϋιΙσ‫ا‬ਘૐ͜‫͛܄‬Ȃ୆૓ळ༾໦‫΀̫̤ͥͅا‬άΐͿΥΞͻ΃σଷࢄ‫͈ࢹܥ‬ٜଢ଼࣐̞ͬ̽̀ͥȃˎȅୈঊ‫͈ړ‬ξΣȜ·̈́ࢹ௮ȁሶ඿൲໤ୈঊ‫͉ړ‬Ȃ฼ତఘΊΦθ̦ίυΗηϋ̽̀ࣞ͢ͅ
ഽͅާੀ̱̤̀ͤȂ֚໐ͅΪΑΠϋ̦ॼͥȃୈঊ·υζΙϋ଎ȇES ळ༾̥ͣ neural crest ͈໦‫ږ͈ࠏا‬ၛȃES ळ༾ͬྫࠬୄ෽ူ̳̭ͥ͂́Ȃ૰ࠐષ๧̥ͣ̈́ͥ sphere ࠁ଼࣐̞ͬȂ̯ͣͅ BMP4 ੜၑͤ͢ͅ neural crest ळ༾ͬ໦‫̵̯ا‬Ȃࣸٔळ༾͈໦‫ͬا‬া̱̹ȃ͈ಎ́Ȃఘळ༾߿͈ΪΑΠϋͬ঵̾ႀ֖͈ਹါ଻ٜͬଢ଼̱̞̀ͥȃˏȅES ळ༾ͬဥ̞̹ Neural Crest ۚळ༾͈ਏၛ͂ठ୆֓ၷ͈͒؊ဥȁES ळ༾̥ͣ neural crest ۚळ༾ͬ‫ږ‬ၛ̱Ȃအș̈́ळ༾ͅ໦‫̵̯ا‬Ȃठ୆֓ၷ͈͒؊ဥͬদ͙ͥȃड߃͈యນഎ̈́ა໲
Inoue H, Ohnishi Y, Nakajima M, Kakudo M, Nozaki M. A novel function of EpCAM in oral squamous cell carcinoma
cells under anchorage-independent conditions. Int J Oncol. 2011 Dec; 39 (6): 1401-5.

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ȁۜஅછ͉Ȃພࡔఘ̷͈͂ۜஅచય̜́ͥ਽৽͈͂௖ࡽ‫଼ͤ͢ͅ߸۾‬ၛ̳ͥȃ̱̹̦̽̀Ȃۜஅછ͈อછι΃ΣΒθ͂ພఠ̷̤͍͈͢হၷ༹ͬࡄ‫͉̹̳͛ͥͅݪ‬Ȃພࡔఘুఘ͈ٜଢ଼͂਽৽௰͈ཡࢄ‫͈ࢹܥ‬ٜଢ଼̦ਹါ̭͉̈́͂ুྶ
̜̦́ͥȂ̯ͣͅ൩͙ࣺͭ́ພࡔఘ͂਽৽ࡢఘ͈͂௖ࡽैဥٜͬଢ଼̳̭ͥ͂͜ຈါ̯͂ͦͥȃ̭͈̹͉͛ͅႉ઄́
͈ΟȜΗ͈ಇୟ͂͂͜ͅȂ൲໤৘ࡑٜͥ͢ͅଢ଼͂࠿બ͉Ȃഐ൚̈́యఢ৘ࡑ༹༷̦̞̹̈́͛Ȃະ‫خ‬๰͈͈̜́ͥ͜ȃ൚ࡄ‫ݪ‬ਫ਼͉́Ȃ୭ၛ൚੝ͤۜ͢அછࡄ‫͈ݪ‬ಎ͈́൲໤৘ࡑ͈ਹါ଻ͬ෇ে̳ͥ͂͂͜ͅȂ̷͈ͦͣ৘ࡑ̦հ஠́ୃ
‫̥̱͜ͅږ‬ഐୃ࣐̭̦ͩͦͥ͂ͅຈါ̜́ͥ͂ࣉ̢̞̀ͥȃུঔ୭͉Ȃ࣭ඤ́࿷͈֚ۜஅ൲໤৘ࡑঔ୭̱͂̀઎გ
42 ාͅ୭ၛ̯ͦȂশయͅ௲؊̱̹‫ͬא׋‬࿒ঐ̱̾̾Ȃۜஅછࡄ‫̞̤̀ͅݪ‬ఱ̧̈́࿨‫ͬڬ‬౜̞௽̫Ȃࣽ඾ͅঢ̽̀

ါ̞ͥȃȁঔ୭͉ఱ̧̩ 2 ͈̾ߊ֖Ȃӱۜஅব֗৘ࡑߊ֖ (BSL2, BSL3)ȂӲSPF ব֗৘ࡑߊ֖Ȃͅ໦̧̫̭̦ͥ͂́ͥȃۜஅব֗৘ࡑߊ֖͈͒໤ৗ͈੄̱ව͉ͦȂpass-through Ηͼί͈ࣞգ૊‫ܨ‬྽޿‫ͬܕ‬೒̢̱̱̥࣐̞̀̈́ΏΑΞθ̞̈́̽̀ͥͅȃ̹ۜ͘அব֗৘ࡑߊ֖͉Ȃߗ಺ۖ๵֮͂͂͜ͅգͅ༗̭̾͂́؄அ͈ςΑ·ͬड઀ࡠͅဲ̢Ȃ̯ͣͅෳ‫ܨ‬
͉ HEPA έͻσΗȜᚹً̯̭ͦͥ͂́Ȃ‫ٸ‬໐͈͒ພࡔఘ͈ၗ८ͬཡ̞̞́ͥȃષܱ͈ΏΑΞθͤ͢ͅȂۜஅ൲໤͈ব֗͂৘ࡑ̦հ஠̢࣐ͥͅঔ୭̞͂̈́̽̀ͥȃ৘ष͈ঔ୭ঀဥ̜̹͉̽̀ͅȂӱ‫߱֗ޗ‬ႯȂӲ൲໤৘ࡑْࠗ੥͈೹੄͂ૣऔȂӳ೰‫ܢ‬എ̈́๷୆໤‫ڠ‬എκΣΗςϋΈ൝ͤ͢ͅȂഐୃ̈́൲໤͈ব֗͂৘ࡑ഼̦࣐̠̞ͩͦͥ͛̀ͥ͢ȃȁঔ୭ࢹ଼A ൓ȇࣞഽհ஠ব֗৘ࡑߊ֖Ȫ଎ 1ȫȂۜஅব֗৘ࡑߊ֖ȂSPF ব֗৘ࡑߊ֖Ȃ;΍Άব֗৒B ൓ȇSPF ব֗৘ࡑߊ֖
ࡄ‫ٽݪ‬ါ
ȁۜஅછͅࡠ̴ͣ୆ྵ‫̞̤̀ͅݪࡄڠش‬Ȃ໦ঊτασ͈́ࡄ‫ͬض଼ݪ‬൲໤ࡢఘτασ́৘ࡑȆ࠿બ̳̹͈ͥ͛֒ഥ
ঊழ̢͙۟൲໤͈ਹါ଻̦ఱ̧̈́๤ਹͬ୸̠̞͛ͥ̈́̽̀ͥ͢ͅȃ൚ঔ୭͉́֒ഥૂ༭৘ࡑΓϋΗȜ͂‫ވ‬൳̱̀Ȃ୆૓ࢥ‫ڠ‬Ȇอ୆ࢥ‫ܖͬڠ‬๕̱̹͂֒ഥঊழ̢۟൲໤͈ैୋܿ੅͈ࡄ‫ݪ‬Ȇ‫ٳ‬อ࣐̠ͬ͂͂͜ͅȂӱΠρϋΑΐͿΣΛ
·൲໤͈ैୋȂӲΦΛ·ͺ;ΠȆΦΛ·ͼϋ൲໤͈ैୋȂӳࡐ๷਎ୈͥ͢ͅࠏൡ֋঵ȂӴ൲໤ࠏൡ͈ൄࠫ༗ం̈́̓Ȃड୶౤͈ܿ੅ͬဥ̞̹൲໤৘ࡑ͈̹͈͛ࡄ‫ݪ‬঑‫̞࣐ͥ̀̽ͬ׳‬Ȫນ 1ȫȃນĲĪġঔ୭̤̞̀ͅैୋȆ༗ం̯̹ͦζ;Α͈ࠏൡତ‫ۼܢ‬ĲĺĺĶĮĳıııĳııĲĮĳııĴ
ĳııĵĮĳııķ
ĳııĸĮĳııĺ
ĳıĲıĭĳıĲĲŵŰŵŢŭ଎ˍȫࣞഽհ஠൲໤ব֗৘ࡑ৒ȪA ൓ 1 ‫ٴ‬ȫΨͼ΂ΓȜέΞͻτασ 3 ͈ۜஅ৘ࡑ̢̦࣐ͥ
ࣞഽ‫ࡏܓ‬ພࡔఘ൲໤৘ࡑ৒̜́ͥȃུ৘ࡑ৒͈
၌ဥͤ͢ͅȂଅછ࢓଻੄ࠬ෎͈ພࡔఘౙၗ଼ࢗͅ ̱Ȃ· υ ͼ Μ έ Ϳ σ Π μ ΋ ή ພȂATLȂ
AIDS ͈̈́̓ພࡔ֦ঊͅ‫̳ͥ۾‬൲໤৘ࡑ̦հ஠
̥̾‫̢࣐ͥͅڲ׫‬ȃ

ŕŨζ;Α
ĳĳĹ
Ĳıĵ
ĵĴ
ĳĳ
ĴĲ
ĵĳĹ

ŌŐζ;Α
Ķı
Ķĸ
ķĺ
ĸĵ
ĸĺ
Ĵĳĺ

ൄࠫ༗ం
ĳķĲ
ĵĵĴ
ĴĴĲ
ĳĲķ
ĳĳı
ĲĵĸĲ

ŕňĭġΠρϋΑΐͿΣΛ·ļġŌŐĭġΦΛ·ͺ;ΠĶĺঔ୭‫ٽ‬ါȁResearch Facilities້௺ࡄ‫ݪ‬ঔ୭ȟ‫ވ‬೒ࡄ‫ݪ‬ঔ୭ۜஅછˠ˪˝ΙΛί‫ٳ‬อΓϋΗȜࡄ‫ݪ‬ΈσȜίΓϋΗȜಿġĩ ࠳ Ī ‫ޗ‬਎ġ ၑ‫ڠ‬ฎআȁ࿤ോȁฎ੩‫ޗ‬ġ֓‫ڠ‬ฎআȁ؈ॄȁఱٚ੎‫ޗ‬਎ġĩ ࠳ Īġ֓‫ڠ‬ฎআȁᩊനȁ‫֚ܮ‬ȁພࡔఘ͈঵̾ພࡔ଻֒ഥঊ̦ۜஅచય͈਽৽ळ༾̤̞̀ͅอ࡛̯̭ͦͥ͂̽̀ۜ͢ͅஅછ଼͉ၛ̳ͥȃۜஅછ͈อછ‫͂ࢹܥ‬ພఠ͈ၑٜ͉Ȃۜஅછ͈ཡࢄ༹͂হၷ༹͈‫ٳ‬อͅਹါ̜́ͥȃ̷͈̹͉͛ͅȂພࡔఘ͈֒ഥঊ̦਽৽͈
ळ༾ಎ́อ࡛̯ͦ̀ພࡔ଻ͬอ‫ॽ̳ͥܞ‬ழ͙̺̫̩́̈́Ȃ਽৽௰͈ཡࢄ‫̞֒̀̾͜ͅࢹܥ‬ഥঊτασٜ́ଢ଼̳̭ͥ
̦͂ຈါ͂̈́ͥȃ̳̻̈́ͩȂພࡔఘুఘ͈֒ഥঊȪΊΦθȫ͈͙̩́̈́Ȃ਽৽֒ഥঊȪΊΦθȫ͈ۜஅͅ؊൞̱̹
֒ഥঊอ࡛ΩΗȜϋ͈་൲ͬમळٜͅଢ଼̳̭̦ͥ͂‫ͥͦͣ͛ݥ‬ȃȁུΓϋΗȜ͉Ȃ̭͈࿒എͬో଼̳̹࣭ͥ͛ͅඤ́࿷͈֚ۜஅછͬచય̱̹͂ DNA ΙΛίΓϋΗȜ̱͂̀໹଼ 16ාഽͅอ௷̱̹ঔ୭̜́ͥȃུঔ୭͈́ࡄ‫ٳݪ‬อ͉ո‫̥༷͈ͣ࢜̾ˎ͈ئ‬ଔૺ̱̞̀ͥȃȪˍȫȁDNA ΙΛίȪDNA ζͼ·υͺτͼȫͬဥ̞̹֒ഥঊอ࡛͈༫‫گ‬എȆ࿌္എٜ̈́ଢ଼ȇȁུΓϋΗȜͅ୭౾̱̹ࣞྟഽ಼઀߿ DNA ͺτͼٜଢ଼ΏΑΞθͬߐঀ̱̀ΪΠȂζ;ΑȂۜஅఘ͈̈́̓֒ഥঊ͈
อ࡛ΩΗȜϋͬତྔࡢ͈֒ഥঊ͈อ࡛་൲ͬ൳শͅ۷ख़̱̦ٜ̈́ͣଢ଼
̳ͥȃ֚૗߿ DNA ζͼ·υͺτͼȪͺέͻιΠςΛ·Α২ȫ̤͍͢ඵ૗߿ DNA ζͼ·υͺτͼȪͺΐρϋΠ২ȫȂΐͿΦΩȜσ஖าͺτͼȪ२຅τͼπϋ২ȫ̴͈̞̞ٜͦ̾̀͜ͅଢ଼̦ଔૺ̧̠́ͥ̈́͢ఘ
ଷͬົ̞̞̀ͥȃΠρϋΑ·ςίΠȜθٜଢ଼̽̀͢ͅࡄ‫ݪ‬చય̱͂̀
ࣆࣺ̹ͤͦ֒͘ഥঊ̞͉̾̀ͅȂςͺσΗͼθ őńœ ٜଢ଼͞΢Φ΃;ϋΗȜ̽̀͢ͅࡢș͈দၳ̤̫ͥ֒ͅഥঊอ࡛ၾ͈་൲ͬમळٜͅଢ଼
̧́ͥΏΑΞθ͜๵̢̞̀ͥȃ༷֚ȂۜஅȆ྾‫ע‬ΏΑΞθ͈஖఼എΠρϋΑ·ςίΠȜθٜଢ଼༹͈‫ٳ‬อུ͜ΓϋΗȜ͈ࡄ‫ݪ‬ΞȜζ͈͌͂̾଎ˍȇࣞྟഽ಼઀߿ DNA ͺτͼٜଢ଼ΏΑΞθ̱͂̀ଔૺ̱̞̀ͥȃ֚႕̱͂̀ȂDNA ζͼ·υͺτͼٜଢ଼ͬ؊ဥ
̱̀Ȃ૧̹̈́ࠬ‫ ס‬RNA ૷౯ΏΑΞθͬࢹಃ̱Ȃ̷ͦͬඳহ଻ࠬ‫׶ۯ‬͈ພ֦֒ഥঊٜଢ଼͞૷౯ζȜ΃Ȝ൳೰̈́̓ͅ؊ဥ̳̞̠ͥ͂৘ဥഎ̈́
ࡄ‫͜ݪ‬ജ‫̞̱ͥ̀ٳ‬ȃȁȁȁȁ
Ȫˎȫȁৗၾ໦ଢ଼‫ͬܕ‬ဥ̞̹ΗϋΩ·ৗอ࡛͈༫‫گ‬എȆ࿌္എٜ̈́ଢ଼ȇȁ֒ഥঊอ࡛ͤ͢ͅॲ୆̯ͦͥΗϋΩ·ৗ͈༫‫گ‬എٜ̈́ଢ଼͜ਹါ̈́‫ه‬ఴ̜́ͥȃུΓϋΗȜͅ୭౾̱̹ MS/MS ߿ৗၾ໦ଢ଼௡౾ͬߐঀ̱̀
ΪΠȂζ;ΑȂۜஅఘ̦̈́̓ॲ୆̳ͥΗϋΩ·ৗ‫͈ۼ‬௖ࡽैဥ͞ਘૐ
൲ఠ͈ٜ̈́̓ଢ଼ͬଔૺ̳ͥȃड߃͉́Ȃৗၾ໦ଢ଼௡౾ͬဥ̞̹ۜஅછ
͈૷౯ܿ੅̦̈́̓ૺ̞࡛ͭ́ͥેͬ‫͙ۻ‬Ȃड୶౤͈ࡄ‫ͅݪ‬చ؊̧́ͥ
ΏΑΞθ͜๵̢̭ͥ͂́Ȃඊু̈́ۜஅఘ࠿౶ΏΑΞθ͈‫ٳ‬อ͜ণ࿤ͅ

଎ˎȇMS/MS ߿ৗၾ໦ଢ଼௡౾

වͦ̀ࡄ‫̞ͥ̀͛ૺͬݪ‬ȃड߃͈యນഎ̈́ა໲
1. Okuzaki D, Kimura S, Yabuta N, Oomine T, Nojima H. LeukoCatch, a quick and efficient tool for the preparation ofleukocyte extracts from blood. BMC Clin Pathol. 2011 Aug 17;11(1):9.
2. Okuzaki D, Fukushima T, Tougan T, Ishii T., Kobayashi S, Yoshizaki K, Akita T, and Nojima H. GenopalTM: anovel hollow fiber array for focused microarray analysis. DNA Res., 2010 Dec;17(6):369-79.
3. Tougan T, Okuzaki D, Nojima H. Chum-RNA allows preparation of a high-quality cDNA library from a single-cell
quantity of mRNA without PCR amplification. Nucleic Acids Res. 2008 Sep;36(15):e92.
4. Kobayashi S, Ito A, Okuzaki D, Onda H, Yabuta N, Nagamori I, Suzuki K, Hashimoto H, Nojima H. Expression
profiling of PBMC-based diagnostic gene markers isolated from vasculitis patients. DNA Res. 2008 Aug;15(4):253-65.
5. Tougan T, Onda H, Okuzaki D, Kobayashi S, Hashimoto H, Nojima H. Focused microarray analysis of peripheralmononuclear blood cells from Churg-Strauss syndrome patients. DNA Res. 2008 Apr 30;15(2):103-14.
ķıঔ୭‫ٽ‬ါȁResearch Facilities້௺ࡄ‫ݪ‬ঔ୭ȟ‫ވ‬೒ࡄ‫ݪ‬ঔ୭ঔ୆ఘ؊൞֒ഥঊٜଢ଼ΓϋΗȜ୭‫ٽ‬ࡄ‫ݪ‬ΈσȜίɃ֒ഥঊ٨་൲໤ै଼໐࿝Ʉ
ȁ‫ޗ‬਎ġ
࿪‫ڠ‬ฎআȁȁȁ‫ؖ‬໐ȁੳȁඅහ੎‫ޗ‬਎ġ෠‫ڠ‬ฎআȁȁȁ५ᣵȁ֚ຳȁ੩‫ޗ‬Ȫ࠳ȫġ֓‫ڠ‬ฎআȁȁȁႱႊȁ‫ܧם‬ȁඅහ੩‫ޗ‬ġ୆ྵ‫ڠش‬ฎআȁષനȁ੕Ƀ൲໤֋঵Ȇ‫ۯ‬ၑ໐࿝Ʉȁ‫ޗ֥ݖ‬਎ġ֓‫ڠ‬ฎআȁȁȁ५ఆȁࡄ֚ȁ੎‫ޗ‬਎Ȫ࠳ȫġ࿪‫ڠ‬ฎআȁȁȁչ୼ȁୃ૽ȁ੩‫ޗ‬Ȫ࠳ȫġ࿪‫ڠ‬ฎআȁȁȁ֙౓ȁիঊɃ‫ވ‬൳ࡄ‫ݪ‬ଔૺ໐࿝Ʉ
ȁ‫ޗ֥ݖ‬਎ġၑ‫ڠ‬ฎআȁȁȁ‫܊‬ாȁဢ֚჊ȁ‫ޗ֥ݖ‬਎ġ֓‫ڠ‬ฎআȁȁȁ‫݌‬നȁૺ઎ȁ੩‫ޗ‬Ȫ࠳ Īġၑ‫ڠ‬ฎআȁȁȁࢃ൥ȁೄ‫ݛ‬ȁඅහ੩‫ޗ‬Ȫ࠳ Īġ ୆ྵ‫ڠش‬ฎআȁऎ൥ȁဉ࢖ါɃ୆ఘ؊൞ٜଢ଼໐࿝Ʉ
ȁ‫ޗ‬਎Ȫ࠳ȫġ֓‫ڠ‬ฎআȁȁȁૣၻȁ୓౳ȁ‫ޗ‬਎Ȫ࠳ȫġ֓‫ڠ‬ฎআȁȁȁ࿐‫ئ‬ȁΗυ;ȁ‫ޗ‬਎Ȫ࠳ȫġ֓‫ڠ‬ฎআȁȁȁ࣏ଳȁંȁ‫ޗ‬਎Ȫ࠳ȫġ֓‫ڠ‬ฎআȁȁȁ݊౓ȁ૾ȁ‫ޗ‬਎Ȫ࠳ȫġၑ‫ڠ‬ฎআȁȁȁ‫ؖ‬നȁٗ૽ȁ‫ޗ‬਎Ȫ࠳ȫġ֓‫ڠ‬ฎআȁȁȁࣞாȁ૝ࢨȁ‫ޗ‬਎Ȫ࠳ȫġၑ‫ڠ‬ฎআȁȁȁ࿤ോȁฎȁ੩‫ޗ‬Ȫ࠳ȫġ࿪‫ڠ‬ฎআȁȁȁ֔ષȁೄგȁ୆ఘ͉ఉ̩͈֒ഥঊ͈൱̧̽̀͢ͅࢭુ଻̦༗̹̞͈֚ͦ̀ͥ̾ΏΑΞθ̜́ͤȂ֒ഥঊ͈‫ܥ‬ෝ։ુ̦ఉ̩͈৖
ພͅ‫۾‬ဓ̱̞̀ͥȃਲ̽̀Ȃ૧̹̈́হၷ༹͈‫ٳ‬อ͈̹͉͛ͅ৖ພͅ‫۾‬Ⴒ̳ͥ֒ഥঊ͈‫ܥ‬ෝ̷͈͂ͦͣ௖ࡽ‫ͬ߸۾‬౶̭̦ͥ͂ਹါ̜́ͥȃ̱̥̱Ȃྚ̺ఉ̩͈֒ഥঊ͈‫ܥ‬ෝ͉ະྶ̜́ͤȂ৖ພͅ‫۾‬ဓ̳ͥ֒ഥঊ͈࿨‫̦ڬ‬ఘࠏഎٜͅ
ଢ଼̯̹ͦ႕͉ઁ̞̈́ȃȁ֒ഥঊ‫ܥ‬ෝ͈ٜଢ଼͉֒ͅഥঊ٨་൲໤̦ޭ͛̀ခဥ̜́ͤȂ৖ພ͈อછ‫̱̥̀ͣͅྶͬࢹܥ‬ခ࢘̈́হၷ༹ͬ‫ږ‬ၛ̳̹͉ͥ͛ͅȂழ૕എ֒ͅഥঊ٨་൲໤ͬैୋ̳ͥ͂͂͜ͅȂِ̦࣭ඊু͈୆໤঩࡙ͬ‫ږ‬༗̳̭̦ͥ͂‫̜ͥ́ྩݢ‬ȃ̳́ͅ੨‫͉࣭́ٸ‬஻࿪ͅ‫̳ͥ۾‬౶എ़ॲࡀ͈‫ږ‬༗ͬැ൮ͅఱၾ͈֒ഥঊ٨་ζ;Α͈ैୋίυΐͿ·Π̦ই̽̀͘
̤ͤȂ்ِ̦࣭̤̞̀͜ͅ‫ͅݢ‬ఘࠏഎ̈́ఘଷͬା๵̱̫̞̈́ͦ͊̈́ͣ̈́ȃȁ̷̭́Ȃ̭ͦ́͘ͅఉ̩͈֒ഥঊ٨་ζ;Αͬैୋ̱̹৘ୡ͈ཅີ̈́ఱिఱ‫ڠ‬๷୆໤ພࡄ‫ݪ‬ਫ਼Ȫ๷ࡄȫȂ൐‫ނ‬ఱ‫ڠ‬֓‫ݪࡄڠش‬ਫ਼Ȫ֓‫ࡄش‬ȫȂߧུఱ‫ڠ‬୆ྵ঩࡙ࡄ‫ݪ‬Ȇ঑‫׳‬ΓϋΗȜȪ୆ྵ঩࡙ΓϋΗȜȫ̦ඊুͅ‫ٳ‬อ̱̹࿹̹ͦܿ੅
ͬ௖ࡽͅ೹‫̠ࣣ̱͂͂͜ͅރ‬Ȃచયͬࣆ̭͙̦ͤ̈́ͣ࢘ၚഎ̥̾ࠏൡഎ֒ͅഥঊ٨་ζ;Αͬैୋ̳ͥȃ̯ͣͅȂ̷̸͈ͦͦ̾͜࿹̹ͦພఠٜଢ଼ΏΑΞθͬ௖ࡽ၌ဥ̳̭ͥ͂́Ȃِ̦࣭͈୆ྵ‫͈ݪࡄ͈ڠش‬อജͅ঩̳ͥயͬಃ̩ȃȁ֓‫̦͉ͭ́ࡄش‬྾‫̳֒ͥ۾ͅע‬ഥঊ߲̞̾̀ͅȂ̷̱̀୆ྵ঩࡙ΓϋΗȜ͉́ඳহ৖ۛͅ‫̳֒ͥ۾‬ഥঊ߲̞̾ͅ
̀Ȃ̷̱̀๷ࡄ͉́୆૓։ુȆۜஅȆͺτσΆȜ྾‫ע‬ͅ‫̳֒ͥ۾‬ഥঊ߲̞̾̀ͅέ΁Ȝ΃Α̜ͬ̀̀ࡄ‫ͬݪ‬࣐̠ ( ଎ 1)ȃ̹͘‫͈̾͜۾ܥڎ‬ಿਫ਼̤ͬࡽ̞ͅ၌ဥ̱
̦̈́ͣȂ̷̸̦ͦͦඅ‫̹̱ا‬໦࿤͈֒ഥঊ٨་ζ;Α͈ैୋ࣐̠ͬȃං̹ͣͦ֒ഥঊ٨་ζ;Α͉‫̦۾ܥڎ‬Ⴒࠈ̱̹࢘ၚഎٜ̈́ଢ଼ΏΑΞθ̥̫̭ͥ͂ͤ͢ͅͅ৖
ພ͈͂Ⴒ‫̳̥ͥͣͅྶͬ۾‬ȃ̭͈̠̈́͢ࡄ‫ͬݪ‬೒̱̀Ȃ૧̹̈́হၷ༹͈‫ٳ‬อͅ঩̳ͥ‫ܖ‬யഎ̈́౶ࡉͬಇୟ̱Ȃ̯ͣͅ؊ဥ͚̫̹ͅΠρϋΑτȜΏο΢σࡄ‫̠࣐ͬݪ‬ȃ̹͘Ȃࠫ‫̱̀͂ض‬ఉ̩͈֒ഥঊ٨་൲໤ͬैୋ̳̭ͥ͂ͤ͢ͅȂِ̦࣭ඊু͈
֒ഥঊ঩࡙ͬ‫ږ‬༗̳ͥ͂͂͜ͅȂ৖ພ͈૧ܰহၷ༹͞
૧࿪͈‫ٳ‬อ̫̹࢜ͅ‫ܖ‬யࡄ‫̠࣐̈́ͬݪ‬ȃ଎ Ĳȁະළછ͈ٜྶ̫̹࢜ͅΑΠρΞΐȜड߃͈యນഎ̈́ა໲
1. Tokuhiro K, Ikawa M, Benham AM, Okabe M. Testis specific PDILT is required for quality control of sperm
membrane protein ADAM3 and male infertility. Proc Natl Acad Sci U S A. 2012 March 6; 109 (10): 3850-3855.
2. Inoue N, Satouh Y, Ikawa M, Okabe M, Yanagimachi R. Acrosome-reacted mouse spermatozoa recovered from theperivitelline space can fertilize other eggs. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20008-11.
3. Kumasawa K, Ikawa M, Kidoya H, Hasuwa H, Saito-Fujita T, Morioka Y, Takakura N, Kimura T, Okabe M.
Pravastatin induces placental growth factor (PGF) and ameliorates preeclampsia in a mouse model. Proc Natl Acad
Sci U S A. 2011 Jan 25;108(4):1451-5.
4. Isotani A, Hatayama H, Kaseda K, Ikawa M, Okabe M. Formation of a thymus from rat ES cells in xenogeneic nude
PRXVHļUDW(6FKLPHUDVGenes Cells. 2011 Apr;16(4): 397-405.
5. Ikawa M, Tokuhiro K, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M. Calsperin is atestis-specific chaperone required for sperm fertility. J Biol Chem. 2011 Feb 18;286(7):5639-46.
ķĲঔ୭‫ٽ‬ါȁResearch Facilities້௺ࡄ‫ݪ‬ঔ୭ȟ‫ވ‬೒ࡄ‫ݪ‬ঔ୭๷ࡄηνȜΐͺθ৒ȁȁಿࢩ༭տ֥ಿ ĩ ࠳ Ī ‫ޗ‬਎ȁၑ‫ڠ‬ฎআȁ࿤ോȁฎঔ୭‫ٽ‬ါ
ȁఱिఱ‫ڠ‬๷୆໤ພࡄ‫ݪ‬ਫ਼͉ಅ๶ࠫ‫ݪࡄړ‬ਫ਼͂ఱिඅਂ๧ໂພࡄ
‫ݪ‬ਫ਼ͬ໵̵̀઎გ˕ා (1934) ͅ୭ၛ̯̹ͦȃ஻ၛ 70 ਔාܱͬැ
̱̀๷ࡄηνȜΐͺθͬ୭౾ْ̳̭̦̯ͥ͂ࠗͦȂ2010 ා 12 ࠮
17 ඾͈‫܁ٳ‬৆ͬ̽̀͜ୃ৆ͅ‫̹̱܁ٳ‬Ȫ଎ˍȫȃ‫ڠ‬ඤ৪Ȃ‫ٸڠ‬৪͂͜ͅ໹඾͈ಱ 9:00~17:00 ́͘ুဇͅ੄ව̧ͤ́ͥȪවાྫၳȫȃ̹͘਋ັ́༿ྴನͅྴஜܱͬව̳ͦࣧ‫̈́ل‬ΩϋέτΛΠ̦ίτΔϋΠ̯ͦͥȃ̳́ͅ‫ˍ܁ٳ‬ාոඤ֚ͅ୷ྴոષ͈ြང৪͈ྴஜͬ
༿ྴನܱͅश̯̹ͦȃȁ๷ࡄ͈อટ͉઎გ 4 ාȪ1929ȫ̯̥͈͖ͥͅȃఱि֓‫ش‬ఱ‫ڠ‬Ȫ઎଎ˍȇ‫܁ٳ‬৆ஜ͈๷ࡄηνȜΐͺθ

გ 6 ාఱिೱ࣭ఱ‫ۯ֊ͅڠ‬ȫ͈නུಿ२჊Ȇ‫ڠ‬ಿ͂౓࢛ᦂඵȆळ޿ࠬୄ‫ޗڠ‬਎͉Ȃఱि͞૰࡫̦΋τρȆβΑΠ͈̈́̓‫ٸ‬ြഥஅພ͈૟ව࿝࡫̜̹̭͂̈́ͤ̾̾̽͂ͬ૤෻̱̞̹̀ȃఱୃ 12 ා͈‫۾‬൐ఱૼब͈‫̜̀̽߱͜ޗ‬Ȃ‫۾‬ୌȪඅͅఱिȫͅ๷୆໤ພͅ‫̳۾‬ͥ௙ࣣഎࡄ‫ͬ۾ܥݪ‬୭ၛ̳ͥຈါ଻ͬࢩ̩୰̞̀ٝͤȂ൚শ͈ఱिຸ౶মড়ന஝२჊͞ఱि़‫͉ͅٮ‬ୟޭഎ̈́‫ފ‬ႁͬါ୏̱̹ȃ५࢛࡚൸ঙ͉Ȃ̭͈ါ୏ͅ؊̢̀ 20 ྔ‫׫‬Ȫ࡛ह͈ତ‫ͅ׫ؙ‬௖൚ȫͬ‫̱ັܙ‬Ȃ̭ͦͤ͢ͅ઎გ 9 ාȪ1934ȫ2 ࠮Ȃఱिঌཤߊ൴ോୌ಴ 3๔౷ͅࡄ‫ݪ‬ਫ਼ུ‫̹̱ࢥੋ̦܁‬ȃ൴ോ΅λϋΩᾼ͉‫ͅܡ‬ಅ๶ࠫ‫ړ‬ࡄ‫ݪ‬ਫ਼Ȫಅ๶হֲ‫מ‬࿝ঙ͈‫ັܙ‬ȫ͂ఱिඅਂ๧ໂພࡄ‫ݪ‬ਫ਼Ȫඈ঎‫ັܙ͈ز‬ȫ̦̜ͤ‫ڰ‬อ̈́ࡄ‫ڰݪ‬൲࣐̞̹̦ͬ̽̀Ȃ̭ͦͣˎ‫۾ܥ‬ͬ໵̵̀઎გ 9 ාȪ1934ȫ9 ࠮ 17 ඾ೂ႓ 270 ࣢ͤ͢ͅȂఱिೱ࣭଎ˎȇಅ๶ࠫ‫ݪࡄړ‬ਫ਼͈ۭโ̤͍͢ಅ๶হֲ‫מ‬࿝ঙ͈ȁȁȁ‫ޝ‬௨Ȫऒȇల Ĳı యȂֲȇల ĲĲ యȫఱ‫້ڠ‬౾͈๷୆໤ພࡄ‫ݪ‬ਫ਼ۗଷ̦࢖ື̯ͦȂུࡄ‫ݪ‬ਫ਼̦อ௷̳ͥ
ͅঢ̹̽ȃȁ๷ࡄηνȜΐͺθ͈ಎ͉ͅಅ๶ࠫ‫ݪࡄړ‬ਫ਼͈୭ၛͅ଄ႁ̯̹ͦಅ๶হֲ‫מ‬࿝ঙȪऒȇల 10 యȂֲȇల 11 యȫ͈‫ޝ‬௨Ȫ଎ˎȫ͞ΡͼΜͤ͢‫̹̯ͦັܙ‬΋Λγ͈ࡐ๷‫ޢ‬Ȫ଎ˏȫȂ٬ဢ൴ୋȪඅಕȫ͈ͼϋέσ΀ϋΎȪ଎ːȫ͂
SARS ;ͼσΑȪ଎ˑȫ͈࿅߿̦̈́̓ജা̯̞ͦ̀ͥȃȁમळ͉γȜθβȜΐȪhttp://museum.biken.osaka-u.ac.jp/ȫͬ४ચ͈̭͂ȃ଎ˏȇ΋Λγ͈ࡐ๷‫ޢ‬ķĳ଎ːȇ٬ဢ൴ୋ͈ͼϋέσ΀ϋΎ;ͼσΑ͈࿅߿଎ˑȇ٬ဢ൴ୋ͈ ŔłœŔ ;ͼσΑ͈࿅߿ঔ୭‫ٽ‬ါȁResearch Facilities້௺ࡄ‫ݪ‬ঔ୭ȟ‫ވ‬೒ࡄ‫ݪ‬ঔ୭
ঔ

ಎ؇৘ࡑ৒

୭
‫ٽ‬

৒ȁȁಿ

‫ޗ‬਎ȁၑ‫ڠ‬ฎআȁ‫ؖ‬നȁٗ૽੩‫ޗ‬ȁ࿪‫ڠ‬ฎআȁ൐५ȁ૯ඵါȁಎ؇৘ࡑ৒͉઎გ 34 ාஜࢃȂ৘ࡑ‫̦ܕܥ‬ະ௷̱̞̹̀শ‫ͅܢ‬Ȃ‫ވ‬೒́ঀဥ̧́ͥ‫ݪࡄڎͬܕܥ‬৒̥ͣ঵̻‫ͤܙ‬Ȃ௖ࡽ͈༒ܽͬ଎
ͥ࿒എ́୭ၛ̯̹ͦȃ࡛ह͉́Ȃအș̈́ୈྟȆࣞ଻ෝ̈́ࡄ‫ܕܥݪ‬̦୭౾̯ͦȂ̞̾́͜ঀဥ‫خ‬ෝ̈́ેఠ̞̈́̽̀ͥͅȃ৽ါ̈́ࡄ‫ݪ‬‫͉̱̀͂ܕܥ‬Ȃ໦ၗဥ಼‫׿‬૤‫ܥ‬Ȃ൫ً߿̤͍͢௢औ߿ഩঊࡐ๷‫ޢ‬Ȃζͼ·υΘͼΓ·ΏοϋτȜΎȜࡐ๷‫ޢ‬ȂΓσΕȜΗȜȂίρΑηΡু൲໦ၗ௡౾ȂDNA ΏȜΉϋ΍ȜȂৗၾ໦ଢ଼௡౾̦̈́̓୭
౾̯̞ͦ̀ͥȃ̷ͦͅ‫̢̀ح‬Ȃ‫ס‬ఘಊள͈‫ুͬݯރ‬൲‫̹̱ا‬ఱ߿ळ༾༗ంΗϋ·৒Ȃඅ೰‫ڠا‬໤ৗͬ৾ͤե̠̹͈͛৘ࡑ৒̈́̓͜
ۖ๵̱̞̀ͥȃ౜൚͈ܿ੅৪͉‫͈ܕܥ‬༗৿Ȇ‫ۯ‬ၑ̺̫̩́̈́Ȃ૧වࡄ‫ݪ‬৪ͅచ̱͈̀‫֗ޗ‬Ȇ߱Ⴏͬ໦౜̱̞̀ͥȃ̹͘ȂΓσΕȜΗȜͥ͢ͅळ༾͈໦ْȂৗၾ໦ଢ଼௡౾ͥ͢ͅ౩ฒ
ৗ͈൳೰Ȃഩঊࡐ๷‫ͥ͢ͅޢ‬۷ख़Ȃ̤͍͢ȂDNA ΏȜΉϋ΍Ȝͥ͢ͅ‫ܖ؂‬෻Ⴅࠨ೰̞͉̾̀ͅȂ਋ుުྩ̱͂̀Ȃਫ਼ඤ͈ࡄ‫ݪ‬৪̥ͣջှ̯̹ͦ΍ϋίσ͈ٜଢ଼ͬయ࣐̱̞̀ͥȃࣽࢃȂ৘ࡑ‫ף͉ܕܥ‬șໝॠ‫̱ا‬Ȃࡄ‫ݪ‬৪ࡢ૽͉́ఉ
ਅ႒͈৘ࡑ‫ͬܕܥ‬ௌै̧̩̩̹́̈́̈́̽̀ͥ͛Ȃષܱ͈̠̈́͢਋ుުྩͬͤ͢ఉ̩͈‫ͬܕܥ‬చય̱̹͈͂͒͂͜‫ڐ‬ْ̬̦ͥࠗૺ̞ͭ́ͥȃ༶ৣ଻൳պࡓள৘ࡑ৒৒ȁȁಿ‫ޗ‬਎ȁၑ‫ڠ‬ฎআȁ‫ؖ‬നȁٗ૽੩‫ޗ‬ȁ࿪‫ڠ‬ฎআȁ൐५ȁ૯ඵȁ൚৘ࡑ৒͉֓‫ڠ‬എࡄ‫̞̤̀ͅݪ‬༶ৣ଻൳պࡓளͬဥ̞ͥ৘ࡑ࣐̠̹͈ͬ͛ঔ
୭̱͂̀Ȃࡄ‫ݪ‬ਫ਼͈଑ന΅λϋΩΑ֊ഢฺ̞ͅȂ઎გ 42 ාͅࡄ‫ݪ‬ਫ਼ུ‫߃ͅ܁‬୪̱ RI ‫ވ‬൳৘ࡑ৒̱͂̀୭౾̯̹ͦȃ઎გ 54 ා͉ͅཤ‫ވٴˍ܁‬൳ྫ޿ RI ৘ࡑ৒Ȃ઎გ 58 ාۜͅஅછ‫ވ‬൳৘ࡑ൓ RI ৘ࡑ৒Ȃ໹଼ 10 ාͅධ‫܁‬౷‫ٴˍئ‬Cs ΄ϋζ஌ચৣ৒Ȃ̯ͣͅ໹଼ 19 ා͉֒ͅഥૂ༭৘ࡑΓϋΗȜ RI ৘ࡑ৒

137̦‫̹̽ͩح‬ȃ̹͘Ȃ໹଼ 22Ȃ23 ාഽ͉ͅჇ‫ ̹͈͛اݤ‬RI ‫ވ‬൳৘ࡑ৒Ȃཤ‫ˍ܁‬‫ވٴ‬൳ྫ޿ RI ৘ࡑ৒Ȃ֒ഥૂ༭৘ࡑΓϋΗȜ RI ৘ࡑ৒ͬ෱গ̱Ȃ૧̱̩྾‫ע‬‫ڠ‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜ൓˕‫ ͅٴ‬RI ৘ࡑ৒ͬ୭౾̱̹ȃ̹͘Ȃධ‫͈܁‬٨ಃฺ̞ͅȂ137Cs ΄ϋζ஌ચৣ৒͉ཤ‫֊ͅٴˍ܁‬୭̱̹ȃ༶ৣ஌‫ۯ‬ၑߊ֖ඤͅ
͉༶ৣ଻൳պࡓளಡ௬৒Ȃ෱‫ܤ‬໤༗‫ۯ‬৒Ȃ૆‫ا‬୭๵‫ঀ͍ݞ‬ဥ̳ͥાਫ਼̜́ͥ৘
ࡑ৒͂‫ڎ‬ਅࡄ‫ݪ‬࿒എ̵̜̹ͩͅ༶ৣ஌௶೰‫ܕܥ‬৒Ȃ෽ူ৒൝̦୭̫̞ͣͦ̀ͥȃ༶ৣ஌‫ۯ‬ၑߊ֖͈͒වప৒͉ࡢ૽஌ၾࠗ๔࣢ͤ͢ͅਬಎ‫ۯ‬ၑ̯̤ͦ̀ͤȂ༶ৣ଻൳պࡓள͈ঀဥ͈ܱ჏൝͜΋ϋάνȜΗ‫ۯ‬ၑ̯ͦȂհ஠଻ͬ༗঵̱̞̀ͥȃ໹଼ 23 ාഽ͈ഴ჏̯̹ͦ༶ৣ஌ުྩਲম৪͉ 223 ྴ͈͖ͤͅȂ൚ࡄ‫ݪ‬ਫ਼̤̞̀ͅਹါ̈́࿨‫̞̱̹ͥ̀ضͬڬ‬ȃķĴঔ୭‫ٽ‬ါȁResearch Facilities້௺ࡄ‫ݪ‬ঔ୭ȟ‫ވ‬೒ࡄ‫ݪ‬ঔ୭ۜஅછ‫ވ‬൳৘ࡑ৒৒ȁȁಿ‫ޗ‬਎ȁ֓‫ڠ‬ฎআȁ‫؂‬നȁోဎȁ൚৘ࡑ৒͉ 1983 ාͅଅછ࢓଻੄ࠬ෎ȪHFRSȫ;ͼσΑͬ৾ե̠ঔ୭̱͂
̀࠺ಃ̯̹ͦȃΪΠ྾‫ע‬ະ஠;ͼσΑȪHIVȫͬ‫̀͛܄‬Ȃ‫ࡏܓ‬ഽ͈̞ࣞȪ·ρΑ 3ȫພࡔ๷୆໤ͬ৾եུ̠ࡄ‫ݪ‬ਫ਼͈́ࡄ‫͓̳͉̀ݪ‬൚৘ࡑ৒࣐́ͩͦ̀
̞ͥȃ൚৘ࡑ৒͉໹࿂ୟ 550 Ԧͬခ̳ͥˏ‫́࠺ٴ‬Ȃ୆໤‫ڠ‬എब‫ٺ‬ȪΨͼ΂ΧΎȜΡȫͬཡগ̳̠ͥ͢୭̯̞ࠗͦ̀ͥȃ‫ڎ‬৘ࡑ৒͉΀ͺυΛ·ͤ͢ͅ‫ٸ‬໐͂‫ڞ‬ၗ̯ͦȂ৘ࡑ৒ඤ͉́‫ٸ‬ĺඤ͈‫ܨ‬ၠͬ‫ږ‬༗̱̞̀ͥȃۜஅછ৘ࡑ
ௌै͉հ஠΅λΫΥΛΠඤ࣐̞́Ȃෳ‫଻͉ࣞܨ‬ෝέͻσΗȜ̽̀͢ͅᚹً
྽޿̯ͦͥȃ‫ڎ‬৒ͅ΂ȜΠ·τȜήͬ୭౾̱Ȃ৘ࡑঀဥ໤͉ۖ஠྽޿ͬঔ
̱̹ࢃͅ෱‫̞̱ͥ̀ܤ‬ȃఉအ̈́ພࡔఘͬ൳শ৾ͤͅե̢̠ͥ͢໹଼ 17 ාഽ
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଎ȁ੥ȁ৒
৒ȁȁಿ

‫ޗ‬਎ȁၑ‫ڠ‬ฎআȁཱ֔ȁੇࢤȁ๷ࡄ଎੥৒͉๷୆໤‫ڠ‬Ȇ྾‫ͬڠע‬ಎ૤ͅȂ‫۾‬Ⴒ̳ͥळ༾‫ڠ‬Ȃ֒ഥ‫ڠ‬Ȃழ૕‫ڠ‬Ȃอ୆‫ڠ‬Ȃ୆‫ڠا‬Ȃ࿪ၑ‫ڠ‬Ȃພၑ‫ڠ‬Ȃळ޿‫ڠ‬Ȃਆន‫ڠ‬൝͈଎੥Ȇ‫੅ڠ‬ॠধͬ৽ͅਓਬ̱̞̀ͥȃಎ́͜‫ܙ‬୆ಖ‫͈߸۾ڠ‬௬੥͉Ȃఈ‫̜ͤ́͘۾ܥ‬ਫ਼௬̯̞̞ͦ̀̈́
͈͜͜ఉ̩Ȃ‫ڠ‬ඤ‫͈ٸ‬ఉ༷࿂̥ͣ၌ဥ̯̞ͦ̀ͥȃȁဏࣣ߿୆ྵ‫ڠش‬௙ࣣࡄ‫ݪ‬൓૧‫ฺ̞ͅא‬Ȃ2007 ා 12 ࠮̥ͣධ‫ ܁‬1F ͅ‫ب‬଎੥৒ͬ‫ٳ‬৒̱̞̹̦̀Ȃ2010 ා 7 ࠮Ȃఛૼ٨ਘࢥম̦ਞၭུ̱̹‫ ܁‬1F ͅ૧୭̯̹ͦ଎੥৒͒֊ഢ̱̹ȃ‫ب‬଎੥৒͉๱ુͅ਀‫̹̹̺͛̽ޛ‬Ȃ൚ࡄ‫ݪ‬ਫ਼́อ࣐̯̹ͦո‫੅ڠ͈ٸ‬ॠধ̞͉̾̀ͅȂ1991 ාոࣛอ࣐໦̥͈ͣਫ਼௬̹͂̈́̽ȃ࡛ह͈௬੥ତ͉࿩ 1 ྔ 3 ୷ॗȂ೰‫ܢ‬എͅ਋ව̱̞̀ͥॠধ͉Ȃ؎໲ধ࿩ 34 ΗͼΠσȂგ໲ধ࿩ 25 ΗͼΠσ̜́ͥȃ૧಍ո‫͈ٸ‬ॠধ͉Ȃཤ‫ ܁‬1F
͈ாࡩͅภ‫̞̱ͥ̀ݑ‬ȃ଎੥৒́ਫ਼௬̱̞̀ͥ঩ၳ͉Ȃ້௺଎੥‫΂͈܁‬ϋρͼϋ࿒჏ΏΑΞθͅഴ჏̯̤ͦ̀ͤȂ࣭ၛૂ༭‫ݪࡄڠ‬ਫ਼͈ILLȪInter Library LoanȫΏΑΞθ̽̀͢ͅ஠࣭͈ఱ‫ڠ‬଎੥‫͈͂܁‬௖ࡽ၌ဥ̦‫خ‬ෝ̞̈́̽̀ͥͅȃ଎੥৒‫א׋‬տ֥̱͂̀Ȃ‫ޗ‬਎ 2 ྴȂ੎‫ޗ‬਎ 2 ྴȂ੩‫ ޗ‬1 ྴ̦‫͈א׋‬හͅ൚̹ͤȂ৘ष͈૖ྩ͉ 1 ྴ͈૖֥࣐̞ͤͩͦ̀ͥ͢ͅȃ൚ࡄ‫ݪ‬ਫ਼͈੄ๅ໤ȨAnnual Reports of the Research Institute for Microbial Diseases Osaka UniversityȩȪ2003 ාͤ͢ഩঊ
‫ا‬ȫ͈༎ਬȂͺΛίυȜΡ൝͈ैު͜଎੥৒࣐̞́Ȃ଎੥৒૖̷֥̦͈හͅ൚̹̞̽̀ͥȃķĵ‫۾‬Ⴒঔ୭ȁRelated Facility‫۾‬Ⴒଲ‫ٮ‬ΠΛίτασࡄ‫ݶݪ‬തঔ୭྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜɜུ‫ݶ‬ത͈අ૗͂փ݅
ȁ྾‫ڠע‬έυϋΞͻͺࡄ‫ݪ‬ΓϋΗȜȪImmunology Frontier ResearchCenter : IFReCȫ͉Ȃଲ‫ٮ‬ΠΛίτασ͈ȶ࿒ͅࡉ̢ͥ‫ݶ‬തࠁ଼ȷͬ࿒എ̱̹͂Ȃ໲໐‫ڠش‬જ͈ȶଲ‫ٮ‬ΠΛίτασࡄ‫ݶݪ‬തίυΈρθȷͅन఼̯ͦ໹଼19ා10࠮1඾ͅอ௷̱Ȃ྾‫͈ݪࡄڠע‬ల֚૽৪̜́ͥૣၻ୓౳‫ޗ‬਎̦‫ݶ‬തಿͅਖහ̱̱̹͘ȃȁ྾‫͉͂ڠע‬๷୆໤ۜஅِ̥ͣș͈ఘͬ৿ͥ୆ఘཡࢄ͈ι΃ΣΒθͬࡄ‫ڠ̳ͥݪ‬࿚ఘࠏ̳́ȃ྾‫ע‬ΏΑΞθ͉ۜஅ̱̹ພࡔఘ
ͬ਽৽̥ͣෳੰ̳ͥષ́ຈଌ̜́ͤȂ྾‫ע‬ΏΑΞθ͈։ુ͉Ȃুࡨ྾‫ע‬৖ۛȂ֊૒༌‫୲ݵ‬ȂͺτσΆȜ฽؊̞̹͂̽အș̈́৖͈ۛࡔ֦̳͂̈́ͤ͘ȃȁ྾‫͉ڠע‬Ȃ඾ུ̦ςȜΡ̧̱̹̀ႀ֖͈̜֚̾́ͤȂ̷͈ఉ̩ͬ५ఆဎ֚ࡓ௙ಿ͞‫ུ܅‬಑२ࡓ௙ಿ͉̲̳ͬ͛͂ͥఱिఱ‫ڠ‬͈ࡄ‫ݪ‬৪଼̦̱ଛ̧̬̱̹̀͘ȃIFReC͉Ȃ̭̠̱̹྾‫ࡄڠע‬‫ͤͬ͢ݪ‬อജ̵̯̹ͥ͛Ȃ྾‫ͼ͂ڠע‬ιȜΐϋΈȪْ௨‫ا‬ȫܿ੅Ȃ̯ͣͅΨͼ΂ͼϋέ΁ζΞͻ·ΑȪ୆ఘૂ༭‫ڠ‬ȫ͈͂ဏࣣࡄ‫ͬݪ‬೒̱̀Ȃ൲໤୆ఘඤȪin vivoȫ̤̫ͥͅ྾‫ע‬฽؊ͬ‫خ‬ণ‫ا‬Ȃ̜̞͉ͥထ௶̳̭ͥ͂ͥ͢ͅ྾‫͈ࠏע‬൲എ̈́஠ཞͬྶ̥ͣ
̳̭ͥ͂ͬͅ࿒എ̱̞̳͂̀͘ȃIFReĆ͉Ȃ20ྴոષ͈ଲ‫ٮ‬ΠΛίτασ͈৽හࡄ‫ݪ‬৪ͬಎ૤ͅȂଲ‫ͅٮ‬႒͈̞̈́ࡄ‫ݶݪ‬ത͈ࢹಃͬ࿒ঐ̱̞̳̀͘ȃࡄ‫ݪ‬৒྾‫ע‬ΈσȜί࣭षন࿚տ֥ٛ‫ݶ‬തಿ໗‫ݶ‬തಿ

‫א׋‬տ֥ٛ

య݈֥ٛুட྾‫ڠע‬Ȫૣၻ ୓౳ȫ৘ࡑ྾‫ڠע‬Ȫा࢛ ঎໲ȫൠङ྾‫ڠע‬Ȫ࿐‫ ئ‬Ηυ;ȫ྾‫ע‬ΏΈ΢σȪ୔൥ ၦȫۜஅພఠȪߧΦ‫ ޡ‬੓ȫ

໦‫ا‬ଷࢄȪࣱॄ ౶ฎȫ྾‫ڠاע‬Ȫ࣏ଳ ંȫςϋΩ‫ݩ‬໦‫ا‬ȪFritz Melchersȫ

྾‫ܥע‬ෝൡࢄ‫ڠ‬Ȫ‫ ུ܅‬಑२ȫક‫ۯا‬྾‫ڠע‬ȪMyoung Ho Jangȫ

྾‫ܥע‬ෝൡࢄ‫ڠ‬Ȫ٨ୃ ࢭࢫȫζρςͺ྾‫ڠע‬ȪCevayir Cobanȫ

྾‫ע‬อ୆‫ڠ‬

χ·Ιϋ‫ڠ‬Ȫ୞֔ ࠲ȫු྄྾‫ڠע‬Ȫಅന ࠩȫ྾‫ע‬ΥΛΠχȜ·Ȫ‫ل‬५ġႁ଼ȫ໦ঊ྾‫ע‬ଷࢄȪ݊౓ ૾ȫ྾‫ܙע‬୆ಖ‫ڠ‬Ȫ५ུ ٗဉȫͼιȜΐϋΈΈσȜί1ळ༾1໦ঊͼιȜΐϋΈȪ࿮ന ຮဎȫ‫ڠا‬໦ঊͼιȜΐϋΈȪ݊౻ გ࿟ȫ୆ఘ‫ܥ‬ෝͼιȜΐϋΈȪ‫ ؖ݌‬༿૶ȫ୆ఘέ΁ΠΣ·ΑȪNicholas Isaac Smithȫ

ळ༾൲ఠ‫ڠ‬Ȫ୞֔ ࿹ȫ྾‫ע‬؊൞Θͼ΢η·ΑȪႝ࿐ ֚ฎȫ‫ڠ֓ړ‬Ȫศᚧ ਜ਼ȫΨͼ΂ͼϋέ΁ζΞͻ·ΑΈσȜίૂ༭ΏΑΞθȪศ ཅȫ྾‫ע‬ΏΑΞθ‫ڠ‬ȪDaron M. Standleyȫ

Ψͼ΂ͼϋέ΁ζΞͻ·ΑΊΦη·ΑȪDiego Miranda-Saavedraȫ
ْܑ৒

মྩ໐࿝ಿ

௙ྩΓ·Ώοϋ
ٛࠗΓ·Ώοϋ

ķĶɜࡄ‫ݪ‬ඤယȆ‫ܢ‬ఞ଼̯ͦͥ‫ض‬ȁ̭ͦ́͘྾‫ͅ৽͉ݪࡄ͈ڠע‬Ȃఘඤͤ৾ͤ͢੄̱̹ळ༾͞෽ူ̱̹ळ༾ͬဥ̧̞࣐̱̹̀ͩͦ̀͘ȃ̭͈̠̈́͢
ࡄ‫༹͉༷ݪ‬Ȃ྾‫ͅڠע‬အș̈́౶ࡉ̹̱̱̹̦ͬͣ͘͜Ȃພࡔఘۜஅͅచ̳ͥ྾‫ע‬؊൞͈ࠫ‫ͬض‬ထ௶̱Ȃ྾‫ע‬؊൞͈஠ఘ௨ͬຝৢ̳ͥ౲‫̵̞̱ͭ̀͘ో͉́͘ͅͅٴ‬ȃ̭̠̱̹‫ه‬ఴͬ࣪໚̳̹͉ͥ͛ͅȂ྾‫ע‬ळ༾͈ͼιȜΐϋΈ͞໦ঊͼιȜΐϋΈ͈‫ٳ‬อ͂୆ఘૂ༭‫ڠ‬ȪΨͼ΂ͼϋέ΁ζΞͻ·Αȫ͈൵ව͉ຈါະ‫̳́ࠧخ‬ȃȁ̯ͣͅȂ୆ఘඤ(in vivo)͈́྾‫ע‬ΏΑΞθ͈‫ܥ‬ෝၑٜͬࡄ‫͉̹̳͛ͥͅݪ‬ȂͼιȜΐϋΈܿ੅͈࢜ષ̺̫́̈́
̩Ȃ໤ၑ‫ڠ‬Ȇ΋ϋάνȜΗ΍ͼ΀ϋΑ͈̈́̓͂྾‫͈ڠע‬ဏࣣࡄ‫̳ͦͣ͛͘ݥ̦ݪ‬ȃIFReĆ͉ଲ‫ٮ‬ΠΛί·ρΑ͈ࡄ‫ݪ‬৪ͬಎ‫̱͂ړ‬Ȃ྾‫ͼ͂ڠע‬ιȜΐϋΈܿ੅ƦΨͼ΂ͼϋέ΁ζΞͻ·Α͈͂‫ވ‬൳ࡄ‫ͬݪ‬೒̱̀Ȃ྾‫͈ע‬௖ࡽैဥȂ྾‫ע‬ळ༾‫͈ا଻ڰ‬Θͼ΢ηΒθͬၑٜ̱Ȃ૧̱̞୽ၞͅ‫̹̞ۜ̿ܖ‬அછχ·Ιϋ͈‫ٳ‬อ͞Ȃအș̈́ۜஅછ͞‫܈‬ͅచ̳ͥ྾‫ע‬ၷ༹͈΋ϋΓίΠ஻੄Ȃুࡨ྾‫ע‬৖͈ۛহၷ༹͈‫ٳ‬อͬ࿒ঐ̱̞̳̀͘ȃ

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࣭ၛ੏۪‫ܕ‬ພΓϋΗȜ߄၊ౣ‫ܢ‬ఱ‫ڠ‬ঢ‫ނ‬സ࣭ൽĲĸĲ࣢

࿻௙ٛພ֭

ཅ୼‫פ‬
֟࿐Ŋń

ঢ‫ނ‬സ

ఱिఱ‫ڠ‬๷୆໤ພࡄ‫ݪ‬ਫ਼૧ࢄ൴޼ঢ‫ࡔع‬಴

ఱिఱ‫ڠ‬ġ଑നġġ΅λϋΩΑ

ཤ୷ၖ

୷ၖಎ؇

ঢఱिߗࢽ

ྴ૰ࣞ௸ൽႹκΦτȜσ֟࿐ि‫୷ݢ‬ၖ஌

࣭ၛྦྷ௼‫ڠ‬ฎ໤‫܁‬࢖‫׬‬൐࢛

ಎ࣭ু
൲৬ൽ
५ന

ঢ෿ന

ঢౠႹ

ྔ

ฎ

֟࿐ঌ

ŋœ൐٬ൽ஌

ಎ؇۪ે஌ྔฎܱැ࢖‫׬‬ཤఱि‫࣐ݢ‬ঢ෿നिఱພ֭ஜ‫פ‬ि‫ނݢ‬സ஌

ྔฎܱැ࢖‫׬‬
‫ٸ‬
ਔൽ
Ⴙ

଑നŊń

ఱिκΦτȜσ
ֳ࿤༏

߃‫ܪ‬
ু൲৬ൽ

ධ֟࿐

ঢ෿ന

ঢ࿝૯ঌ

ঢઐࡔ

࢐೒մඤ
ঢदസୌ
ఱिఱ‫ڠ‬ȁ଑ന΅λϋΩΑཤ୷ၖྔฎܱැ࢖‫׬‬୷ၖಎ؇ಎ࣭ু൲৬ൽఱिߗࢽঢ૧૰࡫ঢ‫ނ‬സ ঢ‫ނ‬സ५നि‫୷ݢ‬ၖ஌

ཤఱि‫࣐ݢ‬

ঢ༯೓

िఱພ֭ஜ

ུ஌

ൽ
൐٬

ঢ࿝૯ঌ

౷‫ئ‬ഘ
ࢄ൴޼஌

ྴ૰ࣞ௸ൽႹۚ஌૧߃‫ুܪ‬൲৬ൽ

૧ఱि
ఱि
Ȫ෿നȫ

ঢ૰࡫

೧‫ޘ‬
ఱि۪ે஌

߃ഘජၻ஌

ঢජၻ

̈́ͭ͊
ŋœ
ධ٬஌

૧ۚ஌

ഛؐ঳
िგ஌

κΦτȜσ

ঢ‫۾‬ୌ࣭षߗࢽȆგ‫ظ‬५ɜഩȁȁȁ৬ȁि‫ݢ‬ഩ৬୷ၖ஌ȁཤ୷ၖ‫ئפ‬৬ȁര༜Ĳĳ໦ɜκΦτȜσȁఱिκΦτȜσदസ஌ȁिఱພ֭ஜ‫ئפ‬৬ȁര༜ĳı໦ɜΨȁȁȁΑȁि‫ݢ‬ΨΑȁ୷ၖಎ؇อȁȶ઀࿤ࡔ൐࣐ȷ
Ȃȶཅ୼‫࣐פ‬ȷྌ͉ȶີআ‫غ‬ब࣐ȷिఱ࢛‫ئ‬৬ȁര༜Ķ໦ȁȁȁȁȁȁȁि‫ݢ‬ΨΑȁ୷ၖಎ؇อȁȶिఱུ໐ஜ࣐ȷྌ͉ȶ֟࿐๼༠ώ‫࣐ݚ‬ȷȁȁȁȁȁȁȁ߃ഘΨΑȁि‫֟ݢ‬࿐ঌ‫פ‬อȶिఱུ໐ஜ࣐ȷȪŋœ֟࿐‫ࠐפ‬ဇȫिఱུ໐ஜ‫ئ‬৬ȁര༜Ĳĳ໦ġĸıմ଑ന΅λϋΩΑඤ଎๷୆໤ພࡄ‫ݪ‬ਫ਼΅λϋΩΑζΛί
Ĵı

ŏ

ཤ࿝

ĳĸ
ĳĹ

ĳĶ
ĲĴ

ĳĳ

ĳĵ
Ĳĳ

Ĳĵ

ĲĹ

ĳķ

Ĳĺ

ĳĺ

̧͊͘༗֗‫׬‬ĳĲ൐࿝߆շٛ‫܁‬

Ĺၑࢥ‫ڠ‬଎੥‫܁‬ňŔņ΋κϋ

ఱिκΦτȜσिఱພ֭ஜ‫פ‬

Ķ
ĲĶ

ĳĴ

୷ၖ࿝
ୌ࿝

Ĵ

Ĳķ
̹̫͈̭༗֗‫׬‬िఱ֓‫ڠ‬໐ஜΨΑ೪୆ྵ‫ڠش‬଎੥‫܁‬िఱུ໐ஜΨΑ೪

ķ

ĳı
ĲĲ

Ĳ

ĺ

ĳ

Ĳĸ
Ȫঢྔฎܱැ࢖‫׬‬ȫ

Ĳı
ĲĲĺ

ୃ࿝

΋ϋαϋΏοϋΓϋΗȜ

ĵ

Ĳĳı
ཤ
୷
ၖ
‫פ‬

ఘ֗‫܁‬

଑ന
΅λϋΩΑ

ྔฎ࢖‫׬‬
ĸ

ि
‫ݢ‬
ཤ
୷
ၖ
஌

िఱධ࢛ΨΑ೪ྔฎ࢖‫׬‬
Ĳĳĺྔฎ࢖‫׬‬ୌ
५ന‫פ‬
५
ന
‫פ‬

ಎ࣭
ু൲
৬ൽ
ఱि
Ⴙ
κΦ
τȜ
σ

Ψͼ΂‫۾‬Ⴒఉ࿒എࡄ‫ݪ‬ঔ୭ŕņōġ Ļ ıķĮķĹĸĳĮĹĳıı
ਯਫ਼ġĻɧĶķĶĮıĹĸĵ
ġġġġġġġġ଑നঌࡣࢺరķĮĳĮĴ

Ĳ ུ

໐

ম

ྩ

‫ܥ‬

ࢹ

଑നঌ५ന‫ݚ‬ȗĴ ֓ ‫ ش ݪ ࡄ ࠏ ڠ‬Ȇ ֓ ‫ ڠ‬໐Ȫ ֓ ‫ ش ڠ‬ȫȗĵ ֓ ‫ ش ݪ ࡄ ࠏ ڠ‬Ȇ ȁ ȗ ȁȪ ༗ ࠲ ‫ ش ڠ‬ȫ
ȗ
Ķ ֓
‫ڠ‬
໐
້
௺
ພ
֭
ȗ
ķ ভ‫شݪࡄڠ‬Ȇভ‫ڠ‬໐Ȇ൳້௺ພ֭ȗĸ ࿪ ‫ ش ݪ ࡄ ڠ‬Ȇ ࿪ ‫ ڠ‬໐ȗĹ ࢥ ‫ ش ݪ ࡄ ڠ‬Ȇ ࢥ ‫ ڠ‬໐
ȗ
ĺ ୆
ྵ
‫ܥ‬
ෝ
ࡄ
‫ݪ‬
‫ش‬
ȗ
Ĳı ૂ
༭
‫ش‬
‫ڠ‬
ࡄ
‫ݪ‬
‫ش‬
ȗ
ĲĲ ఱिఱ‫ڠ‬Ȇ߄ాఱ‫ڠ‬Ȇຩઐ֓‫ش‬ఱ‫ڠ‬Ⴒࣣ઀঱อో‫شݪࡄڠ‬
ȗ
Ĳĳ ๷
୆
໤
ພ
ࡄ
‫ݪ‬
ਫ਼
ȗ
ĲĴ ౩ ȁ ฒ ȁ ৗ ȁ ࡄ ȁ ‫ ݪ‬ȁ ਫ਼ȗĲĵ ೩ ȁ ‫ أ‬ȁ Γ ȁ ϋ ȁ Η ȁ ȜȗĲĶ ρ ΐ ΂ ͺ ͼΕΠ Ȝ ί ௙ ࣣ Γ ϋ Η ȜġȗȗĲķ ۪ ‫ ޏ‬հ ஠ ࡄ ‫ ۯ ݪ‬ၑ Γ ϋ Η Ȝ
ĳ ૽‫شݪࡄڠشۼ‬Ȇ૽‫ڠشۼ‬໐

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ĴġĮġĲ
ĴġĮġĳ
ĳġĮġĲ
ĳġĮġĵ
ĳġĮġĵ

Ĳĸ
ĲĹ
Ĳĺ
ĳı
ĳĲ
ĳĳ
ĳĴ
ĳĵ
ĳĶ
ĳķ
ĳĸ
ĳĹ
ĳĺ
Ĵı

ၣ
‫ڠ‬
୆
Γ
ϋ
Η
Ȝ
୆ ໤ ࢥ ‫ ࣭ ڠ‬ष ࢐ ၠΓϋ Η Ȝ୶ ౤ ‫ͼ ڠ ش‬Φα Ȝ Ώοϋ Γ ϋ Η Ȝႉ ઄ ֓ ࢥ ‫ ڠ‬ဏ ࣣ ࡄ ‫ ֗ ޗ ݪ‬Γ ϋΗ ȜΈυȜΨσ΋ρδτȜΏοϋΓϋΗȜ΍ΑΞͼ΢ΫςΞͻȆΟΎͼϋȆΓϋΗȜτȜΎȜ΀ΥσΆȜ‫ݪࡄڠ‬ΓϋΗȜ྾ ‫ ڠ ע‬έ υ ϋ Ξͻͺ ࡄ ‫ ݪ‬Γ ϋ Η Ȝ
ॲ
ު
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‫ڠ‬
ࡄ
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২
ٛ
ࠐ
फ
ࡄ
‫ݪ‬
ਫ਼
୪
ࣣ
‫ش‬
‫ڠ‬
ࡄ
‫ݪ‬
ਫ਼
಼ ࣞ գ ഩ ঊ ࡐ ๷ ‫ ޢ‬Γϋ Η Ȝ΍ ͼ Ψ Ȝ ι Οͻͺ Γ ϋ Η Ȝ‫ ړ‬໤ ၑ ࡄ ‫ ݪ‬Γ ϋ Η Ȝอ࣐ȁఱिఱ‫ڠ‬๷୆໤ພࡄ‫ݪ‬ਫ਼ࢩ༭տ֥ٛȁɧ565-0871 ଑നঌ५ന‫ݚ‬3๔1࣢
TELȪ06ȫ6877-5111ȪయນȫȁFAXȪ06ȫ6879-8266ȁURL http://www.biken.osaka-u.ac.jp଑നঌ५ന‫ݚ‬
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