Cell Culture-Related Products

CatalogCell Culture-Related Products

The expected delivery price shown in this table is the

Table of Contents

expected delivery price excluding consumption tax.
*From April 1, 2016

Unified product
Code

Product
Code

Eagle’ s MEM Medium①(Sterilizable in Autoclave)302059003

05900

100 g

1 Year1

Eagle’ s MEM Medium① Pack

302081608

08160

For 1L x 10 Packages

2 Years

1

Eagle’ s MEM Medium② (PR-free)(Sterilizable in Autoclave)302059010

05901

100 g

1 Year2

Eagle’ s MEM Medium③ (KM and PR-free)(Sterilizable in Autoclave)302059027

05902

100 g

1 Year2

Dulbecco's Modified Eagle's Medium①

302059157

05915

100 g

1 Year4

Dulbecco's Modified Eagle's Medium②(Sterilizable in Autoclave)302059195

05919

100 g

1 Year5

RPMI 1640 Medium①

302059119

05911

100 g

1 Year

6

RPMI 1640 Medium②(Sterilizable in Autoclave)302059188

05918

100 g

1 Year7

199 Medium

302059096

05909

100 g

1 Year8

Ham F 12 Medium

302059102

05910

100 g

1 Year9

SFM -101 Medium

302059638

05963

For 1 L

1 Year10

Hanks' Solution①

302059058

05905

100 g

2 Years

11

Hanks' Solution (PR-Free)②

302059065

05906

100 g

2 Years11

Dulbecco PBS (-) Powder

302059133

05913

100 g

2 Years

12

Dulbecco PBS (-) Powder Pack

302081905

08190

For 1L x 10 Packs

2 Years

12

Dulbecco PBS (-) Powder Pack

30208192908192

For 10 L x 1 Pack

2 Years

12

Glutamine

302059089

05908

0.3 g

2 Years

12

Product Name

DesiredDelivery Price

Package

Expiration
Date *

Page

Powder Medium

Storage: Powder medium shall be stored in a sealing container and kept in a dry,cool and dark place (2 ~ 10 ° C).

*The Expiration Date indicates a period after manufacturing.

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

Autoclavable,
Kanamycin and Phenol Red Containing, Lglutamine and Sodium Bicarbonate-Free

Eagle’ s MEM Medium

05900

100 g

08160

For 1L x 10 Packs"AccuDia" ①

Eagle's MEM "AccuDia" ①

Eagle's MEM Medium"AccuDia" ① Pack Product Eagle's
MEM "AccuDia"① Pack ProductComposition
Per 9.4 g (1 L)
Sodium Chloride・・・・・・・・・・・・・・・・・・・・・6,800 mg
Potassium Chloride・・・・・・・・・・・・・・・・・・・・・400 mg
Calcium Chloride (Anhydrate)・・・・・・・・・・・・・・・・・200 mg
Magnesium Sulfate (Anhydrate)・・・・・・・・・・・・・・・・93.5 mg
Sodium Dihydrogen Phosphate (Dihydrate)・・・・・・・・・・・ 150 mg
Glucose・・・・・・・・・・・・・・・・・・・・・・・・・1,000 mg
L-Arginine Hydrochloride・・・・・・・・・・・・・・・・・・・126 mg
L-Cysteine Hydrochloride Hydrate・・・・・・・・・・・・・・・31.4 mg
L-Tyrosine・・・・・・・・・・・・・・・・・・・・・・・・・・36 mg
L-Histidine Hydrochloride Hydrate・・・・・・・・・・・・・・・・42 mg
L-Isoleucine・・・・・・・・・・・・・・・・・・・・・・・・・52 mg
L-Leucine・・・・・・・・・・・・・・・・・・・・・・・・・・52 mg
L-Lysine Hydrochloride・・・・・・・・・・・・・・・・・・・・ 73 mg
L-Methionine・・・・・・・・・・・・・・・・・・・・・・・・ 15 mg
L-Phenylalanine・・・・・・・・・・・・・・・・・・・・・・・ 32 mg
L-Threonine・・・・・・・・・・・・・・・・・・・・・・・・・48 mg
L-Tryptophan・・・・・・・・・・・・・・・・・・・・・・・・ 10 mg
L-Valine ・・・・・・・・・・・・・・・・・・・・・・・・・・ 46 mg
Succinic Acid ・・・・・・・・・・・・・・・・・・・・・・・・75 mg
Disodium Succinate (Anhydrate) ・・・・・・・・・・・・・・・・60 mg
Choline Tartrate・・・・・・・・・・・・・・・・・・・・・・・1.8 mg
Folic Acid ・・・・・・・・・・・・・・・・・・・・・・・・・・1 mg
myo-Inositol・・・・・・・・・・・・・・・・・・・・・・・・・ 2 mg
Nicotinamide ・・・・・・・・・・・・・・・・・・・・・・・・ 1 mg
Calcium Pantothenate・・・・・・・・・・・・・・・・・・・・・1 mg
Pyridoxal Hydrochloride ・・・・・・・・・・・・・・・・・・・・ 1 mg
Riboflavin ・・・・・・・・・・・・・・・・・・・・・・・・・ 0.1 mg
Thiamine Hydrochloride・・・・・・・・・・・・・・・・・・・・ 1 mg
D-biotin ・・・・・・・・・・・・・・・・・・・・・・・・・ 0.02 mg
Kanamycin Sulfate・・・・・・・・・・・・・・・・・・60 mg (potency)
Phenol Red ・・・・・・・・・・・・・・・・・・・・・・・・・ 6 mg

Usage
Dissolve 9.4 g of this powder in distilled water
to make a total volume up to 1,000 mL andsterilize the medium solution in an autoclave at
121 ° C for 15 minutes. After autoclavesterilization, the medium is cooled to room
temperature and then an appropriate amountof 10% aqueous sodium hydrogen carbonate
solution, which has been sterilized before by
autoclaving under an airtight condition, isadded to it (When 12.5 ~ 22.0 mL is added, thepH at 37 ° C under 5% CO 2 is 7.1 ~ 7.4.). If
not using immediately, the medium is stored ina sealing conatiner and kept in a cool darkplace (2 ~ 10 ° C). Before use, 0.292 g ofseparately filter-sterilized L-glutamine is added
to the medium. Sterile lyophilized glutamine"AccuDia" is convenient for it. An appropriate
amount of serum is added according to the
purpose.
CAUTIONAs this product contains 60 mg (potency) of
kanamycin sulfate/L, it is not necessary to add
antibiotics such as streptomycin or penicillin.

Description
Eagle’ s MEM medium "AccuDia" ① was
prepared following the formulation of MEM
medium published by Harry Eagle in 1959 andwidely used for cell culture. Traditionally, thistype of medium cannot be sterilized by
autoclaving due to degradation and alterationof its components, and has been generaly
sterilized by filtration using membrane filters.
By using a special method, our company has
succeeded in producing a powder medium thatcan be sterilized by autoclaving without
damaging any component of the medium andthe bacterial growth supporting ability.
The medium exhibits excellent
growth-promoting effects on HeLa cells, L cells,
and other cell lines, as well as on unestablishedcells that have been isolated from biologicaltissues.
Storage
This medium powder shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture. (2 Years for Pack
Product).

1

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

Autoclavable,
Kanamycin-Containing,
Phenol Red, L-Glutamine and Sodium

Usage
See Eagle’ s MEM medium "AccuDia" ①.
This medium exhibits excellent
growth-promoting effects on cell lines such as
HeLa cells and L cells, and also on on the cells
not isolated from living tissues.

Bicarbonate-Free

Eagle’ s MEM Medium

05901

100 g

"AccuDia" ②

Eagle's MEM "AccuDia" ②
Composition

Description

The composition of this medium is the samae as that of Eagle’ s MEM medium"AccuDia" ①w ithout phenol red.
For 1 liter = 9.4 g

Storage
This medium powder shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture..
[Representative cells that can be cultured withthis medium]
NIH3T 3/14 -1, WI -38, Vero, HeLa, V 79, RC4,
etc.

Product
Code

Product Name

PackageDesiredDelivery Price

Usage
See Eagle’ s MEM medium "AccuDia" ①．

Autoclavable,
Kanamycin-, Phenol Red, L-Glutamine andSodium Bicarbonate-Free

Eagle’ s MEM Medium"AccuDia" ③

05902

100 g

Eagle's MEM "AccuDia" ③
Composition
The composition of this medium is the samae as that of Eagle’ s MEM medium"AccuDia"① w ithout kanamycin sulfate and phenol red.
For 1 liter = 9.4 g

2

Description
This medium powder shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture.

3

Powder Medium
Product Name

Product
Code

PackageDesiredDelivery Price

L-Glutamine-Containing,
Sodium Bicarbonate-Free

Dulbecco's Modified
Eagle's Medium

05915

100 g

"AccuDia"①
Dulbecco's Modified Eagle
Medium "AccuDia" ①

Usage
Dissolve 10.0 g of this powder medium in
distilled water to make a total volume up to
1,000 mL.
An appropriate amount of sodium bicarbonate
is added to the prepared medium solution (thepH at 37 ° C under 5% CO2 gas is 7.1 ~ 7.4
when 1.0 ~ 1.8 g is added.) and sterilize thesolution by filtration just after complete
dissolution. Advance lowering pH with CO2 gas
before filtration can prevent excessive pH
increases. If not used immediately, theprepared medium solution is stored in a sealing
container and kept in a cool dark place (2 ~ 10
° C). Before use, add appropriate amount ofserum according to purpose.
CAUTION① This product contains L-glutamine and does
not contain sodium bicarbonate.
② This product cannot be sterilized by
autoclaving.

4

Composition

Description

Per 10.0 g (1 L)
Sodium Chloride・・・・・・・・・・・・・・・・・・・・・6,400 mg
Potassium Chloride ・・・・・・・・・・・・・・・・・・・・ 400 mg
Calcium Chloride (anhydrate) ・・・・・・・・・・・・・・・・200 mg
Magnesium Sulfate (Anhydrate)・・・・・・・・・・・・・ ・・97.7 mg
Sodium Dihydrogen Phosphate (Dihydrate) ・・・・・・・・ ・・・125 mg
Iron (III) Nitrate (Enneahydrate)・・・・・・・・・・・・・・・・ 0.1 mg
Glucose・・・・・・・・・・・・・・・・・・・・・・・・・1,000 mg
Sodium Pyruvate・・・・・・・・・・・・・・・・・・・・・・110 mg
L-Arginine Hydrochloride ・・・・・・・・・・・・・・・・・・・ 84 mg
L-Cystine Dihydrochloride・・・・・・・・・・・・・・・・・・62.6 mg
Glycine ・・・・・・・・・・・・・・・・・・・・・・・・・・ 30 mg
L-Histidine Hydrochloride Hydrate・・・・・・・・・・・・・・・ 42 mg
L-Isoleucine・・・・・・・・・・・・・・・・・・・・・・・104.8 mg
L-Leucine・・・・・・・・・・・・・・・・・・・・・・・・104.8 mg
L-Lysine Hydrochloride ・・・・・・・・・・・・・・・・・・ 146.2 mg
L-Methionine・・・・・・・・・・・・・・・・・・・・・・・・30 mg
L-Phenylalanine・・・・・・・・・・・・・・・・・・・・・・・66 mg
L-Serine・・・・・・・・・・・・・・・・・・・・・・・・・・42 mg
L-Threonine ・・・・・・・・・・・・・・・・・・・・・・・ 95.2 mg
L-Tryptophan・・・・・・・・・・・・・・・・・・・・・・・・16 mg
L-Tyrosine Disodium・・・・・・・・・・・・・・・・・・・・89.5 mg
L-Valine・・・・・・・・・・・・・・・・・・・・・・・・・93.6 mg
Choline Tartrate・・・・・・・・・・・・・・・・・・・・・・ 7.2 mg
Folic Acid・・・・・・・・・・・・・・・・・・・・・・・・・・4 mg
Nicotinamide・・・・・・・・・・・・・・・・・・・・・・・・ 4 mg
Calcium Pantothenate ・・・・・・・・・・・・・・・・・・・・ 4 mg
Pyridoxal Hydrochloride・・・・・・・・・・・・・・・・・・・・4 mg
Riboflavin・・・・・・・・・・・・・・・・・・・・・・・・・0.4 mg
Thiamine Hydrochloride・・・・・・・・・・・・・・・・・・・・4 mg
Myo-Inositol・・・・・・・・・・・・・・・・・・・・・・・・7.2 mg
Phenol Red・・・・・・・・・・・・・・・・・・・・・・・・・5 mg
L-Glutamine ・・・・・・・・・・・・・・・・・・・・・・・ 584 mg

Dulbecco's Modified Eagle Medium "AccuDia"① i s a powder medium prepared by ourcompany using a special method according to
the medium formula developed by R. Dulbecco
et al.
The advantage of this medium is thatneutiritions necessary for the growth ofcultured cells are rich by increasing the aminoacid concentration in the Eagle’ s MEM by
about twice and the vitamin concentration by
about four time. In particular, since the growth
can be maintained even after virus is applied, it
is suitable for use in research fields using
viruses. This medium is also widely used inprimary cultures of a variety of cells as an
improved Eagle’ s MEM containing thenon-essential amino acids glycine and serine,
pyruvate, an important component ofcarbohydrate metabolism, trace amounts ofiron ions,etc.
Storage
This powder medium shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture.

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

Autoclavable,
L-Glutamine and Sodium Bicarbonate-Free

Du Dulbecco's Modified
Eagle's Medium

05919

100 g

"AccuDia" ②

Dulbecco's Modified Eagle
M edium"AccuDia"②

Usage
Dissolve 9.5 g of this powder medium in
distilled water to make a total volume up to
1,000 mL. After dissolving on a magnetic stirrer
for about 30 minutes, the medium solution issterilize by autoclaving at 121 ° C for 15
minutes. After autoclaving, the medium iscooled to room temperature, ant then an
appropriate amount of 10% aqueous sodiumhydrogen carbonate solution, which has beensterilized before by autoclaving under airtightcondition, is added (When 12 ~ 20 mL isadded, the pH at 37 ° C under 5% CO 2 gas is
7.1 ~ 7.4.). If not using immediately, theprepared medium solution shall be stored in asealing container and kept in a cool dark place(2 ~ 10 ° C). Before use, 0.584 g of separately
filter-sterilized L-glutamine is added. An
appropriate amount of serum is added
according to the purpose.
CAUTION
This medium does not contain L-glutamine or
sodium bicarbonate.

Composition

Description

Per 9.5 g (1 L)
Sodium Chloride・・・・・・・・・・・・・・・・・・・・・6,400 mg
Potassium Chloride ・・・・・・・・・・・・・・・・・・・・ 400 mg
Calcium Chloride (Anhydrate) ・・・・・・・・・・・・・・・・ 200 mg
Magnesium Sulfate (Anhydrate)・・・・・・・・・・・・・・・ 97.7 mg
Sodium Dihydrogen Phosphate (Dihydrate) ・ ・・・・・・・・・ 140 mg
Iron (III) Nitrate (Enneahydrate) ・ ・ ・・・・・・・・・・・・・ 0.1 mg
Glucose ・ ・・・・・・・・・・・・・・・・・・・・・・・ 1,000 mg
Sodium Pyruvate ・ ・・・・・・・・・・・・・・・・・・・・ 110 mg
Succinic Acid・・・・・・・・・・・・・・・・・・・・・・・ 106 mg
Disodium Succinate (Anhydrate)・・・・・・・・・・・・・・・16.2 mg
L-Arginine Hydrochloride・・・・・・・・・・・・・・・・・・・84 mg
L - Cysteine Hydrochloride Hydrate・・・・・・・・・・・・・・70.3 mg
Glycine・・・・・・・・・・・・・・・・・・・・・・・・・・ 30 mg
L – Histidine Hydrochloride Hydrate ・・・・・・・・・・・・・・ 42 mg
L - Isoleucine ・・・・・・・・・・・・・・・・・・・・・・ 104.8 mg
L - leucine ・・・・・・・・・・・・・・・・・・・・・・・ 104.8 mg
L - Lysine Hydrochloride・・・・・・・・・・・・・・・・・・146.2 mg
L-Methionine・・・・・・・・・・・・・・・・・・・・・・・・30 mg
L-Phenylalanine・・・・・・・・・・・・・・・・・・・・・・・66 mg
L - Serine ・・・・・・・・・・・・・・・・・・・・・・・・・ 42 mg
L-Threonine ・・・・・・・・・・・・・・・・・・・・・・・ 95.2 mg
L – Tryptophan・・・・・・・・・・・・・・・・・・・・・・・ 16 mg
L-Tyrosine Disodium・・・・・・・・・・・・・・・・・・・・ 89.5 mg
L - Valine・・・・・・・・・・・・・・・・・・・・・・・・・93.6 mg
Choline Tartrate ・・・・・・・・・・・・・・・・・・・・・・ 7.2 mg
Folic Acid・・・・・・・・・・・・・・・・・・・・・・・・・・ 4 mg
Nicotinamide ・・・・・・・・・・・・・・・・・・・・・・・・ 4 mg
Calcium Pantothenate ・・・・・・・・・・・・・・・・・・・・ 4 mg
Pyridoxal Hydrochloride・・・・・・・・・・・・・・・・・・・・ 4 mg
Riboflavin・・・・・・・・・・・・・・・・・・・・・・・・・0.4 mg
Thiamine Hydrochloride・・・・・・・・・・・・・・・・・・・・4 mg
Myo – Inositol・・・・・・・・・・・・・・・・・・・・・・・ 7.2 mg
Phenol Red・・・・・・・・・・・・・・・・・・・・・・・・・5 mg

Dulbecco's Modified Eagle Medium"AccuDia"②i s an autoclavable powder
medium tha is prepared by our company using
a special process according to the medium
formulation developed by R. Dulbecco.
The advantage of this medium is that it is richin the neutritions necessary for the growth ofcultured cells by increasing the amino acidconcentration in the Eagle’ s MEM by about
twice and the vitamin concentration by aboutfour time. In particular, since the growth can be
maintained even after virus is applied, it issuitable for use in research fields using viruses.
The medium is used in cultures of a variety ofprimary cells and cell lines as an improved
Eagle’ s MEM containing the non-essential
amino acids pyruvate, iron ions, etc..
Storage
This powder medium shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture.

5

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

L-glutamine-Containing Sodium
Bicarbonate-Free RPMI 1640
05911

Medium"AccuDia" ①

100 g

RPMI 1640 Medium "AccuDia" ①

Usage
Dissolve 10.4 g of this powder medium in
distilled water to make a total volume up to
1,000 mL. If the prepared solution is turbid ordifficult to dissolve, lowering the pH to about
6.0 with a CO 2 gas makes the solution soluble
and become transparent. After dissolution, an
appropriate amount of sodium bicarbonate(When 0.8 ~ 1.6 g is added, the pH at 37 ° C
under a 5% CO 2 gas is 7.1 ~ 7.4) is added tothe medium, and the meidumu is sterilized
immediately by filtration. If not using
immediately, the prepared medium solution isstored in a sealing conatiner, and kept in a cooldark place (2 ~ 10 ° C). Before use, anappropriate amount of serum is added
according to purpose.
CAUTION① This product contains L-glutamine and does
not contain sodium bicarbonate.
② This product cannot be sterilized by
autoclaving.

Composition
Per 10.4 g (1 L)

Sodium Chloride・・・・・・・・・・6,000 mg
Potassium Chloride・・・・・・・・ ・400 mg
Calcium Nitrate (Tetrahydrate) ・・・・ 100 mg
Magnesium Sulfate (Anhydrate) ・・・48.84 mg
Disodium Hydrogen Phosphate(Dihydrate)

RPMI 1640 medium "AccuDia" ① was a powder
medium that is prepared by our company using
L-Tyrosine・・・・・・・・・・・・・・20 mg a special method in accordance with the
L-Valine・・・・・・・・・・・・・・・20 mg suspension culture medium developed by Dr.
D-Biotin ・ ・・・・・・・・・・・・・ 0.2 mg George E Moore, Director of the Roswell Park
Memorial Institute. This medium prevents
Calcium Pantothenate・・・・・・・・0.25 mg decomposition and deterioration of
Choline Chloride・・・・・・・・・・・・3 mg components, which are drawbacks ofFolic Acid・・・・・・・・・・・・・・・1 mg conventional liquid products, and it enables
long-term storage.
Myo-Inositol・・・・・・・・・・・・・ 35 mg With this medium, many people around the
Nicotinamide・・・・・・・・・・・・・ 1 mg world have made a breakthrough in the primaryρ-aminobenzoic Acid ・・・・・・・・・・1 mg culture and maintenance of strain passage ofmouse and human leukemia cells. This medium
Pyridoxine Hydrochloride ・・・・・・・・ 1 mg is also widely used in both basic and clinical
Riboflavin・・・・・・・・・・・・・・0.2 mg settings because it also applicable toThiamine Hydrochloride・・・・・・・・・1 mg suspension culture of nonleukemic cells.
[Representative cells that can be cultured with
Cyanocobalamin・・・・・・・・・・0.005 mg dhis medium]L-glutamine・・・・・・・・・・・・・300 mg Various monoclonal antibody producing strains,
Phenol Red・・・・・・・・・・・・・・5 mg primary cultures of human peripheral blood
lymphocytes, etc.

・・・・・・・・・・・・・・・・・1,004 mg
Glucose ・・・・・・・・・・・・・ 2,000 mg
L-Arginine・・・・・・・・・・・・・ 200 mg
L-asparagine (Monohydrate)・・・・・ 56.8 mg
L-Aspartic Acid ・ ・ ・・・・・・・・・ 20 mg
L-Cystine Dihydrochloride・・・・・ ・65.15 mg
L-Glutamic Acid ・・・・・・・・・・・ 20 mg
Glutathione ・・・・・・・・・・・・・ 1 mg
Glycine・・・・・・・・・・・・・・・ 10 mg
L-histidine・・・・・・・・・・・・・・15 mg
L-Hydroxyproline・・・・・・・・・・・ 20 mg
L-Isoleucine・・・・・・・・・・・・・50 mg
L-Leucine・・・・・・・・・・・・・・50 mg Other substances to be added
L-Lysine Hydrochloride ・・・・・・・・ 40 mg Sodium Bicarbonate・・・・・・・ As Needed
L-methionine ・・・・・・・・・・・・ 15 mg
L-Phenylalanine ・・・・・・・・・・・ 15 mg
L-proline ・・・・・・・・・・・・・・ 20 mg
L-Serine ・・・・・・・・・・・・・・ 30 mg
L-Threonine ・・・・・・・・・・・・・20 mg
L-Tryptophan ・・・・・・・・・・・・・5 mg

6Description

Storage
This medium powder shall be stored in asealing container and kept in dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacturing.

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

Autoclavable,
L-Glutamine- and Sodium Bicarbonate-Free

RPMI 1640 Medium "AccuDia"②

05918

100 g

RPMI 1640 Medium "AccuDia" ②

Usage
Dissolve 10.2 g of this powder medium in
distilled water to make a total volume up to
1,000 mL. After the medium has completely
dissolved, sterilize by autoclaving at 121 ° C for
15 minutes. After sterilization, the meidum iscooled to room temperature and an appropriate
amount of 10% aqueous sodium hydrogencarbonate solution, which has been sterilized
before by autoclaving under airtight condition,is added (When 18 ~ 30 mL is added, the pH at
37 ° C under 5% CO 2 gas is 7.1 ~ 7.4.). If notused immediately, the prepared medium
solution shall be stored in a sealing container
and kept in a cool dark place (2 ~ 10 ° C).
Before use, add appropriate amount of serum
according to purpose.
CAUTION
This product does not contain L-glutamine andsodium bicarbonate.

Composition
Per 10.2 g (1 L)

Sodium Chloride・・・・・・・・・・6,000 mg
Potassium Chloride ・・・・・・・・・ 400 mg
Calcium Nitrate (Tetrahydrate)・・・・・100 mg
Magnesium Sulfate (Anhydrate)・・・ 48.84 mg
Sodium Dihydrogen Phosphate (Dihydrate) 880 mg

Glucose・・・・・・・・・・・・・・2,000 mg
Succinic Acid・・・・・・・・・・・・・46 mg
Disodium Succinate (Anhydrate)・・・・98.85 mg
L-Arginine Hydrochloride・・・・・・・・240 mg
L-Asparagine (Monohydrate)・・・・・・56.8 mg
L-Aspartic Acid・・・・・・・・・・・・ 20 mg
L-Cysteine Hydrochloride Hydrate・・・・72.9 mg
L-Glutamic Acid ・・・・・・・・・・・・20 mg
Glutathione ・・・・・・・・・・・・・・ 1 mg
Glycine ・・・・・・・・・・・・・・・ 10 mg
L-Histidine Hydrochloride Hydrate・・・ 20.3 mg
L-Hydroxyproline・・・・・・・・・・・ 20 mg
L-Isoleucine ・・・・・・・・・・・・・ 50 mg
L-Leucine・・・・・・・・・・・・・・ 50 mg
L-Lysine Hydrochloride・・・・・・・・・40 mg

L-Methionine・・・・・・・・・・・・15 mg
L-Phenylalanine・・・・・・・・・・・ 15 mg
L-Proline・・・・・・・・・・・・・・20 mg
L-Serine・・・・・・・・・・・・・・ 30 mg
L-Threonine・・・・・・・・・・・・ 20 mg
L-Tryptophan・・・・・・・・・・・・ 5 mg
L-Tyrosine・・・・・・・・・・・・・ 20 mg
L-Valine・・・・・・・・・・・・・・ 20 mg
D-Biotin・・・・・・・・・・・・・・0.2 mg
Calcium Pantothenate・・・・・・・ 0.25 mg
Choline Chloride・・・・・・・・・・・3 mg
Folic Acid・・・・・・・・・・・・・・1 mg
Myo-Inositol・・・・・・・・・・・・ 35 mg
Nicotinamide ・・・・・・・・・・・・1 mg
ρ-Aminobenzoic Acid ・・・・・・・・・1 mg
Pyridoxine Hydrochloride ・・・・・・ ・ 1 mg
Riboflavin・・・・・・・・・・・・・0.2 mg
Thiamine Hydrochloride・・・・・・・・1 mg
Cyanocobalamin・・・・・・・・・0.005 mg
Phenol Red・・・・・・・・・・・・・5 mg

Description
RPMI 1640 medium "AccuDia" ②w as a
autoclavable powder medium prepared by ourcompany using a special method in accordancewith the suspension culture medium
formulation developed by Dr. George E Moore,
Director of the Roswell Park Memorial Institute.
This medium is used for primary cells and celllines of mouse and human leukemia cells, aswell as for the culture of KATO-III cells (signet
ring cell carcinoma) and many human cancer
cells.
Containers and storage
This medium powder shall be stored in asealing container and kept in dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture.

Other substances to be added

Sodium Bicarbonate・・・・・・・As Needed
L-Glutamine・・・・・・・・・・・・292 mg

7

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

Sodium Bicarbonate-Free

Medium 199

05909

100 g

"AccuDia"
Medium 199 "AccuDia"

Usage
Dissolve 9.5 g of this powder medium in
distilled water to make a total volume up to
1,000 mL (Do not heat.). An appropriate
amount of sodium bicarbonate is added (When
1.3 ~ 2.2 g is added, the pH at 37 ° C under a
5% CO 2 gas is 7.1 ~ 7.4) and dissolved
completely. Then the prepared medium solution
is sterilized immediately by filtration. Lowering
pH with CO2 gas before filtration can prevent
excessive pH increases. If not used
immediately, the medium solution shall be
stored in a sealing container and kept in a cooldark place (2 ~ 10 ° C). Before use, add
appropriate amount of serum according topurpose.
CAUTION
This product contains L-glutamine and does notcontain sodium bicarbonate.

Composition
Per 9.5 g (1 L)

Sodium Chloride・・・・・・・・・6,800 mg
Potassium Chloride ・・・・・・・・ 400 mg
Calcium Chloride (Anhydrate) ・・・・ 200 mg
Magnesium Sulfate (Anhydrate) ・・・97.7 mg
Sodium Dihydrogen Phosphate(Monohydrate)・・125 mg
Iron (III) Nitrate (Enneahydrate) ・・・ 0.72 mg
Sodium Acetate ・・・・・・・・・・ 50 mg
Adenosine 5 'Triphosphate Disodium ・・ 1 mg
Glucose・・・・・・・・・・・・ 1,000 mg
L-Arginine Hydrochloride・・・・・・・ 70 mg
L-Histidine Hydrochloride Hydrate ・・ 21.9 mg
L-Lysine Hydrochloride・・・・・・・・70 mg
L-Tryptophan・・・・・・・・・・・・10 mg
L-Phenylalanine・・・・・・・・・・・25 mg
L-Methionine・・・・・・・・・・・・15 mg
L-Serine・・・・・・・・・・・・・・25 mg
L-Threonine・・・・・・・・・・・ ・30 mg
L-Leucine・・・・・・・・・・・・・ 60 mg
L-Isoleucine ・・・・・・・・・・・・ 20 mg
L-Valine・・・・・・・・・・・・・・ 25 mg
L-Glutamic Acid・・・・・・・・・・ 66.8 mg
L-Aspartic Acid・・・・・・・・・ ・・ 30 mg
L-Alanine ・・・・・・・・・・・・・ 25 mg
L-Proline・・・・・・・・・・・・・・40 mg
L-Hydroxyproline・・・・・・・・・・・10 mg
Glycine・・・・・・・・・・・・・・ 50 mg
L-Glutamine・・・・・・・・・・・・100 mg
L-Cystine・・・・・・・・・・・・・ 20 mg
L-Tyrosine・・・・・・・・・・・・・ 40 mg
L-Cysteine Hydrochloride Hydrate ・・ 0.11 mg

8

ρ-Aminobenzoic Acid ・・・・・・・ 0.05 mg
D-Biotin・・・・・・・・・・・・・0.01 mg
Calcium Pantothenate ・・・・・・・0.01 mg
Folic Acid ・・・・・・・・・・・・ 0.01 mg
myo-inositol・・・・・・・・・・・ 0.05 mg
Nicotinic Acid・・・・・・・・・・ 0.025 mg
Nicotinamide・・・・・・・・・・ 0.025 mg
Pyridoxal Hydrochloride・・・・・・ 0.025 mg
Pyridoxine Hydrochloride ・ ・・・・ 0.025 mg
Riboflavin ・・・・・・・・・・・・ 0.01 mg
Thiamine Hydrochloride・・・・・・ 0.01 mg
Vitamin A Acetate ・・・・・・・・・ 0.1 mg
L-Ascorbic Acid・・・・・・・・・・ 0.05 mg
α-Tocopherol Disodium Phosphate・・0.01 mg
Calciferol・・・・・・・・・・・・・ 0.1 mg
2-Methyl -1, 4-Naphthokine・・・・・ 0.01 mg
Choline Tartrate・・・・・・・・・・ 0.9 mg
2-Deoxy-D-Ribose ・・・・・・・・・ 0.5 mg
Adenine・・・・・・・・・・・・・・10 mg
Guanine Hydrochloride ・・・・・・・ 0.3 mg
Hypoxanthin・・・・・・・・・・・・0.3 mg
Thymine・・・・・・・・・・・・・ 0.3 mg
Uracil ・・・・・・・・・・・・・・ 0.3 mg
Xanthine・・・・・・・・・・・・・0.3 mg
AMP -2 Na ・・・・・・・・・・・・ 0.2 mg
D-Ribose・・・・・・・・・・・・・ 0.5 mg
Glutathione ・・・・・・・・・・・ 0.05 mg
Cholesterol・・・・・・・・・・・・ 0.2 mg
Polysorbate 80 ・・・・・・・・・・・ 5 mg
Phenol Red・・・・・・・・・・・・・6 mg
Other substances to be added
Sodium Bicarbonate・・・・・・・As Needed

Description
Medium 199 "AccuDia" is a powder medium
prepared by our company using a special
preparation process following the formulationof a synthetic culture solution by Morgan,
Morton and Parker.
The medium contains a variety of amino acids,
vitamins, nucleic acid components and a small
number of intermediate metabolites andothers. Although this medium alone is notpromising for cell growth, it is widely used as acell maintenance medium in polio vaccines andother viral studies. This medium can be used as
an excellent growth medium for primarycultures when serum is added.
Storage
This medium powder shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture.

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

Sodium Bicarbonate-Free

Ham's F 12 Medium

05910

100 g

"AccuDia"
Ham's F 12 Medium "AccuDia"

Usage
Dissolve 10.6 g of this powder medium in
distilled water to make a total volume up to
1,000 mL. At this time, do not apply excessive
heat (37 ° C or higher) to the medium. An
appropriate amount of sodium bicarbonate isadded (When1.2 ~ 2.0 g is added, the pH at 37° C under a 5% CO 2 gas is 7.1 ~ 7.4) anddissolved completely, and then the preparedmedium solution is sterilized immediately byfiltration. Llowering pH with CO2 gas before
filtration can prevent excessive pH increases. If
not used immediately, the prepared medium
solution is stored in a sealing container andkept in a cool dark place (2 ~ 10 ° C). Before
use, add appropriate amount of serum
according to purpose.
CAUTION
This product contains L-glutamine and does notcontain sodium bicarbonate.

Composition
Per 10.6 g (1 L)

Sodium Chloride・・・・・・・・・ 7,599 mg
Potassium Chloride・・・・・・・・・224 mg
Calcium Chloride (Anhydrate)・・・ ・33.31 mg
Magnesium Chloride (Hexahydrate) ・・ 122 mg
Disodium Hydrogen Phosphate (Dihydrate)

・・・・・・・・・・・・・・・・177.97 mg
Copper Sulfate (II) (Pentahydrate)・・0.0025 mg
Zinc Sulfate (Heptahydrate) ・・・ ・ 0.863 mg
Iron (II) Sulfate (Heptahydrate) ・・・ 0.834 mg
Glucose・・・・・・・・・・・・・1,802 mg
L-Arginine Hydrochloride ・・・・・・ 211 mg
L-Alanine・・・・・・・・・・・・・8.91 mg
L-Asparagine (Monohydrate) ・・・・ 15.01 mg
L-Aspartic Acid ・・・・・・・・・・13.31 mg
L-Cysteine Hydrochloride Hydrate ・・ 35.12 mg
L-Glutamic Acid・・・・・・・・・・14.71 mg
L-Glutamine・・・・・・・・・・・・ 146 mg
Glycine・・・・・・・・・・・・・・7.51 mg
L-Histidine Hydrochloride Hydrate・・ 20.96 mg
L-Isoleucine・・・・・・・・・・・・3.94 mg
L-Leucine・・・・・・・・・・・・13.12 mg
L-lysine Hydrochloride ・・・・・・・ 4.48mg
L-Phenylalanine ・・・・・・・・・ 4.96 mg
L-proline・・・・・・・・・・・・ 34.53 mg
L-Serine ・・・・・・・・・・・・ 10.51 mg
L-Threonine・・・・・・・・・・・11.91 mg
L-Tryptophan・・・・・・・・・・・2.04 mg
L-Tyrosine・・・・・・・・・・・・ 5.44 mg
L-Valine・・・・・・・・・・・ ・ 11.71 mg

D-Biotin・・・・・・・・・・・・0.0073 mg
Choline Chloride ・・・・・・・・・・ 14 mg
Calcium Pantothenate ・・・・・・ 0.477 mg
Folic Acid・・・・・・・・・・・・ 1.32 mg
Hypoxanthin・・・・・・・・・・・ 4.08 mg
Myo-Inositol ・・・・・・・・・・・・18 mg
Putrescine Dihydrochloride ・・・・ 0.161 mg
Pyridoxine Hydrochloride ・・・・・ 0.062 mg
Riboflavin・・・・・・・・・・・・0.038 mg
Sodium Pyruvate・・・・・・・・・・110 mg
Thiamine Hydrochloride・・・・・・0.377 mg
Thymidine ・・・・・・・・・・・ 0.727 mg
Cyanocobalamin ・・・・・・・・・ 1.36 mg
Nicotinamide・・・・・・・・・・ 0.037 mg
α-Lipoic Acid ・・・・・・・・・・ 0.206 mg
Linoleic Acid ・・・・・・・・・・ 0.084 mg
Phenol Red ・・・・・・・・・・・ 1.24 mg

Description
Ham F 12 Medium "AccuDia" is a powder
medium prepared by our company using a
special preparation process following the liquidsynthetic medium formulation reported by
Richard G. Ham in 1965.
This medium is excellent for single-cell cultureof mammalian cells and is widely used for
culture of Chinese hamster cells. It also exhibits
excellent growth-promoting effects in primarycultures of various cells including human cells.
Storage
This medium powder shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
1 Year After Manufacture.

Other substances to be added

Sodium Bicarbonate・・・・・・・As Needed

9Powder Medium
Product Name

Product
Code

Package

Serum-Free Medium
Sodium Bicarbonate-Free

SFM – 101 Medium

05963"AccuDia"
SFM -101 "AccuDia"

For 1 L

DesiredDelivery Price

Usage
1. The total amount of one bottle of basic medium(12.5 g) is completely dissolved in about 900 mL of
distilled water. Lowering pH to about 6.0 with a CO 2
gas makes the product more soluble.
2.Ten(10) mL of distilled water is added toSupplement A and Supplement A is dissolved
completely.
3.Total amounts of Supplements A and B are added tothe prepared basic medium and an appropriate
amount of sodium hydrogen carbonate is added(When 1 ~ 1.5g is added to 1 L of medium, the pH of
the medium at 37 ° C under a 5% CO2 2 gas is 7.1~ 7.4). Then distilled water is added to the preparedmedium to make the total volume up to 1,000 mL.
4.After mixing thoroughly, the medium is sterilize
immediately by filtration.
5.When the medium is not used immediately after
preparation, the prepared medium solution is stored
in a sealing container and kept in a cool, dark place(2 ~ 10 ° C).
CAUTION① When sterilizing the medium by filtration, use aprotein low adsorption filter to avoid adsorption ofprotein components to the filter.
② If the dissolved supplement A cannot be used up, it
can be used later if stored at -20 ° C or below.
However, freeze-thaw cycle must be avoided.
③ The storage period for the prepared medium
should be no more than 2 months.

Composition

Description

HSFM -101 medium "AccuDia" is a serum-free[Contents of the Set]
synthetic medium prepared by adding insulin,
Basal Medium・・・・・・・12.5 g (For 1 L) x 1 L-Tryptophan・・・・・・・・・・・・ 7.5 mg transferrin, and monoethanolamine to a basic
Supplement A for 10 mL (For 1 L) x 1
L-Valine・・・・・・・・・・・・・・・48 mg medium, a mixture of equal amounts of RPMI 1640
Supplement B・・・・・・・・・mL (For 1 L) x 1 ρ-Aminobenzoic Acid・・・・・・・・・ 0.5 mg and Eagle’ s MEM to which several components
such as thymidine and selenium have been added
In 12.5g Basal Medium (For 1 L)
D-Biotin ・ ・・・・・・・・・・・・・ 0.2 mg and the amount of amino acids has also been
Sodium Chloride・・・・・・・・・・6,000 mg Choline Bitartrate ・・・・・・・・・・ 0.9 mg readjusted.
Potassium Chloride ・・・・・・・・・400 mg Choline Chloride ・・・・・・・・・・ 26.5 mg 1.
Features
Since this medium does not contain any protein
Calcium Chloride (Anhydrate) ・・・・・100 mg Folic Acid ・ ・・・・・・・・・・・・・ 1 mg
component other than insulin and transferrin, there
is no problem of fluctuation of cell proliferation
Calcium Nitrate (Tetrahydrate): 50.4 mg
myo-Inositol・・・・・・・・・・・・ 18.5 mg
capacity due to lot-to-lot variationf like FBS andMagnesium Sulfate (Anhydrate)・・・・・71 mg Nicotinamide・・・・・・・・・・・・・1 mg
albumin.
Sodium Dihydrogen Phosphate (Dihydrate)
Calcium Pantothenate ・・・・・・・・ 0.6 mg 2.Since the content of protein is low, a purification
procedure is very easy when recovering the cell・・・・・・・・・・・・・・・・ ・・ 75 mg Pyridoxal Hydrochloride・・・・・・・・0.5 mg
producing materials are recovered from the cell
Disodium Hydrogen Phosphate (Dihydrate)
Pyridoxine Hydrochloride ・・・・・・・ 0.5 mg
culture supernatant.
・・・・・・・・・・・・・・・・・・502 mg Riboflavin・・・・・・・・・・・・・0.15 mg 3.Although this medium is prepared for the purpose ofpromoting the proliferation of mouse hybridoma
Sodium Selenite・・・・・・・・・・0.002 mg Thiamine Hydrochloride ・・・・・・・・ 1 mg
cells and improving the antibody productivity, it can
L-Arginine ・・・・・・・・・・・・・ 100 mg Cyanocobalamin・・・・・・・・・・0.004 mg
also be widely applied to cells of a human
lymphocyte system.
L-Arginine Hydrochloride・・・・・・・・78 mg Glucose・・・・・・・・・・・・・ 2,000 mg
4.HAT selection is not necessary when hybridoma cells
L-Asparagine (Monohydrate) ・・・・・ 43.4 mg Sodium Pyruvate ・・・・・・・・・・ 110 mg
are produced using mouse myeloma P3U -1 and NS
L-Aspartic Acid・・・・・・・・・・・・10 mg Succinic Acid ・・・・・・・・・・・ 37.5 mg-1 cells as a parental line,. The hybridoma cells can
be cloned by using these cells as feeder cells.
L-Cysteine Hydrochloride Hydrate ・・・ 15.7 mg Disodium Succinate (Anhydrate) ・・・・ 50 mg
5.Preparation of the medium is easy because it
L-Cystine Dihydrochloride ・ ・・・・・ 32.5 mg Hypoxanthin ・・・・・・・・・・・ 0.025 mg
consists of a basal medium and supplements.
L-Glutamic Acid・・・・・・・・・・・・10 mg Thymidine ・・・・・・・・・・・・ 0.013 mg
CAUTION
L-Glutamine ・・・・・・・・・・・・ 600 mg Glutathione・・・・・・・・・・・・・0.5 mg This basal medium contains sodium selenite, but it is
Glycine・・・・・・・・・・・・・・・10 mg Putrescine Dihydrochloride・・・・・ 0.013 mg not classified as an extra-pharmaceutical toxic
L-Histidine ・・・・・・・・・・・・・ 7.5 mg Dihydroxyethylglycine ・・・・・・・ 1,800 mg substance because it’ s content is extremely low.
(0.00011% or Less).
L-Histidine Hydrochloride Hydrate・・・・ 21 mg Phenolred Sodium Salt・・・・・・・・・5 mg
Storage
L-Hydroxyproline・・・・・・・・・・・ 10 mg
Store in a cool and dark place (2 ~ 10 ° C).
L-Isoleucine・・・・・・・・・・・・・51 mg Supplement A (freeze-Dried Product)
L-Leucine・・・・・・・・・・・・・・51 mg
For 10 mL (For 1 L) Expiration Date
L-Lysine Hydrochloride ・・・・・・・・ 15 mg Insulin (Cattle)・・・・・・・・・・・・10 mg 1 Year After Manufacture.
L-Phenylalanine ・・・・・・・・・・ 23.5 mg Transferrin (Human Holo-Type)・・・・・ 10 mg
L-Proline ・・・・・・・・・・・・・・ 15 mg Supplement B (liquid)
L-Serine ・ ・・・・・・・・・・・・・ 30 mg
5 mL (For 1 L)
L-Threonine・・・・・・・・・・・・・ 49 mg Monoethanolamine・・・・・・・・・・20 mg
L-Tyrosine・・・・・・・・・・・・・・28 mg

10Powder Medium
Product Name

Product
Code

PackageDesiredDelivery Price

9.8 g of this powder medium is dissolved in
distilled water to make a total volume up to
1,000 mL. After complete dissolution, asuitable amount of sodium bicarbonate isadded to the medium solution (When 0.2 ~0.35 g of sodium hydrogen carbonate is added,
the pH is 7.3 ~ 7.6 at 37 ° C in the air phase.)and sterilize it by filtration immediately. If notusing immediately, the prepared medium
solution is stored in a sealing container andkept in a cool dark place (2 ~ 10 ° C).

Sodium Bicarbonate-Free

Hanks' Solution

05905

Usage

100 g

"AccuDia" ①

Hanks ’ Solution "AccuDia" ①

Composition

Description

Per 9.8 g (1 L)
Sodium Chloride・・・・・・・・・・・・・・・・・8,000 mg
Potassium Chloride ・ ・・・・・・・・・・・・・・・ 400 mg
Disodium Hydrogen Phosphate (Dihydrate) ・・・・・・・ 60 mg
Potassium Di-Hydrogen Phosphate (Anhydrate)・・・・・・60 mg
Magnesium Sulfate (Heptahydrate)・・・・・・・・・・ 100 mg
Magnesium Chloride (Hexahydrate)・・・・・・・・・・100 mg
Calcium Chloride (Anhydrate) ・・・・・・・・・・・・ 140 mg
Glucose ・・・・・・・・・・・・・・・・・・・・ 1,000 mg
Phenol Red・・・・・・・・・・・・・・・・・・・・・6 mg

Hanks' solution "AccuDia" □ is a physiologicalbuffered saline solution that contains the
inorganic salts essential for cells to become
isotonic with serum and tissue fluids and also
has a buffering capacity to minimize changes inpH during culture. And It aslo contains glucose,a basic energy source for cells. Furthermore,
phenol red is added as a pH indicator so thatusers can directly observe the decrease in pHof the prepared culture solution
Storage
This powder medium shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date :2 Years After Manufacture.

Product Name

Product
Code

PackageDesiredDelivery Pricephenol red- andsodium bicarbonate-free

Hanks' solution

05906

100 g

"AccuDia" ②Hanks ’ Solution "AccuDia" ②

Composition
Per 9.8 g (For 1 L)
Sodium Chloride・・・・・・・・・・・・・・・・・・・・8,000 mg
Potassium Chloride ・・・・・・・・・・・・・・・・・・・ 400 mg
Disodium Hydrogen Phosphate (Dihydrate) ・・・・・・・・・・ 60 mg
Potassium Di-Hydrogen Phosphate (Anhydrate) ・・・・・・・・ 60 mg
Magnesium Sulfate (Heptahydrate)・・・・・・・・・・・・・100 mg
Magnesium Chloride (Hexahydrate)・・・・・・・・・・・・・100 mg
Calcium Chloride (Anhydrate)・・・・・・・・・・・・・・・・140 mg
Glucose・・・・・・・・・・・・・・・・・・・・・・・・1,000 mg

Usage
Dissolve 9.8 g of this powder medium in
distilled water to make a total volume up to
1,000 mL. After complete dissolution, add asuitable amount of sodium bicarbonate to thesolution (When 0.2 ~ 0.35 g of sodiumhydrogen carbonate is added, the pH is 7.3 ~7.6 at 37 ° C in air phase.), and sterilize
prepared solution by filtration immediately. If
not using immediately, the medium solution
shall be stored in a sealing container and kept
in a cool dark place (2 ~ 10 ° C).

Description
Hanks' solution "AccuDia" ② has the same
composition as that of Hanks’ solution“AccuDia”① without phenol red and is used
for dialysis, immunization experiments, etc.
Storage
This powder medium shall be stored in asealing container and kept in a dry, cool anddark place (2 ~ 10 ° C).
Expiration Date
2 Years After Manufacture.

11

Powder Medium
Product
Code

Product Name

PackageDesiredDelivery Price

1. In case of PBS (-): Dissolve 9.6 g of PBS (-)
powder in distilled water to make a total
volume up to 1,000 mL, and sterilize theprepared medium solution by autoclaving at
121 ° C for 15 minutes or by filtration.
2. In case of PBS (+): Dissolve 9.6 g of PBS (-)
powder in distilled water to make the total
volume up to 800 mL. Two hundred (200)mL of a metal salt solution [Self-preparation:
100mg of Calcium chloride (anhydrrate) and
100mg of magnesium chloride (hexahydrate)
are dissolved in distilled water to make avolume up to 200 mL] is poured into the
prepared medium solution stepwisely whilestirring,. The medium solution thus obtained
is mixed and sterilize by filtration. When themedium solution is sterilized by autoclaving,each solution is sterilized separately andmixed aseptically after cooling sufficiently as
described above.

Dulbecco

PBS (-) powder

05913

100 g

08190

For 1L x 10 Packs

08192

For 10 L x 1Pack"AccuDia"
Dulbecco's PBS (-) "AccuDia"

Dulbecco

PBS (-) powder"AccuDia" Pack Product
Dulbecco's PBS (-) "AccuDia" Pack
Product

Usage

Composition

Description

Per 9.6 g (1 L)
Sodium Chloride ・・・・・・・・・・・・・・・・・・・・8,000 mg
Potassium Chloride・・・・・・・・・・・・・・・・・・・・200 mg
Disodium Hydrogen Phosphate (Anhydrate) ・・・・・・・・・ 1,150 mg
Potassiumdi-Hydrogen Phosphate ・・・・・・・・・・・・・・200 mg

Dulbecco PBS is a physiological buffer salt
solution that contains the inorganic salts
essential for cells to become isotonic withserum and tissue fluids and is prepared to havesufficient buffering capacity in the air phase ata pH suitable for the cells. Generally, PBS (-) isused as a solvent for enzyme such as trypsin ora cell washing solution before enzyme
treatment, and PBS (+) is used as awashingsolution for cells or diluent for drugs.

Note: 1)This medium does not contain Ca + and Mg +, but phosphate can be formed andprecipitated depending on the conditions of the water added.

Storage① PBS (-) powder "AccuDia" shall be stored in asealing container and kept in a dry, cool, anddark place (2 ~ 10 ° C).
② The prepared PBS (-) and PBS (+) shall be
stored in a sealing container and kept in acool dark place (2 ~ 10 ° C).
Expiration Date
2 Years after manufacture.

Product
Code

Product Name

Package

Sterile Freeze-Dried Glutamine "AccuDia"
Glutamine "AccuDia"

05908

0.3 g

DesiredDelivery Price

Usage
Dissolve one vial with 10 mL of sterile distilledwater. Then, required amount of the prepared
solution is added aseptically to a dissolved andsterilized autoclavable medium (ex. Eagle’ s
MEM medium "AccuDia" ①) .
CAUTION
The residual of the partially used L-glutamine
solution can be used later if being
freeze-preserved.

Composition
One vial of this productcontains 0.3 g of sterile-lyophilized L-glutamine.

Description
Storage① This powder product shall be stored in a cooland dark place (2 ~ 10 ° C).
② L-glutamine solution is usable for 6 months
at -20 ° C and for 7 days at 2 ~ 10 ° C.
Expiration Date
2 Years After Manufacture.

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