Application Note
Wnt3a Alternative Peptide [Product ID: PG-008]
14days Stability in Cell Culture Medium at 37℃
Wnt proteins are secreted glycoproteins to control multiple processes of embryonicdevelopment and adult homeostasis and constitute a family of 19 molecular species [1, 2].
As one molecule in the family, Wnt3a is thought to play an important role in acting on
pluripotent stem cells and maintaining the integrity of embryonic and adult tissues [3].
In general, Wnt proteins are lipidated and highly hydrophobic, so they can be isolated onlyin the solution containing some detergents, which eliminate their utilization in various
biological tests. In addition, isolated and purified Wnt proteins become structurally unstableeven under detergent-containing conditions and tend to lose their biological activity within
24 hours under standard cell culture conditions at 37°C [4]. Therefore, for a long-time culture,
the culture should be replaced with fresh medium frequently depending on the used cells
type and experimental conditions.
All the growth factor alternative peptides from PeptiGrowth have unique cyclic structure
understood to be structurally stable and are expected to be stable in the cell culture media[5]. At this testing, time-dependent changes of Wnt3a alternative peptide "PG-008"
concentrations in a liquid medium under standard cell culture conditions (at 37°C) were
determined, demonstrating the PG-008 concentration was apparently maintained as over
80% even after 2 weeks (see below).

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Method: PG-008 (final concentration: 100 nM) was added into the liquid medium (commerciallyavailable DMEM/F-12) and maintained at 37°C for 14 days. Relative concentrations of PG-008
were measured by LC/MS system at several points (at 0, 1, 3, 7, and 14 days).

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Result: Concentrations were shown as maintained at 80% or higher even after 14 days undernormal cell culture conditions (N=3, mean ± SD, in the upper graph).
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Application Note⚫

References：
1) Logan CY, Nusse R. Annu Rev Cell Dev Biol. 2004; 20: 781-810.
2) Saito-Diaz K, et al. Growth Factors. 2013; 31: 1-31.
3) Lu J, et al. Proc Natl Acad Sci U S A. 2006; 103: 5688-5693.
4) Dhamdhere GR, et al. PLoS One. 2014; 9: e83650.
5) Nature Portfolio 2022. A growing market for synthetically produced peptides (nature.com)⚫

Precautions regarding the product：➢Please read the Safety Data Sheet (SDS) carefully prior to use the product.

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To use this product immediately after dissolving is recommended.

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This product is a reagent for research use only. Not to use for any purpose other thanresearch or investigational use.

➢Specifications, contents, appearances, etc. of this product may be changed without notice.

➢Contact us or our sales agents, if with any large amounts or other special specification.

Product lineup
Product code

Factor Name

PG-001

HGF alternative peptide

PG-002

TGFβ1 inhibitor

PG-003

BDNF alternative peptide

PG-004

Noggin-like peptide

PG-005

BMP7 selective inhibitor

PG-006

BMP4 selective inhibitor

PG-007

VEGF alternative peptide

PG-008Wnt3a alternative peptide

PG-009Synthetic EGF (human)

PG-010

TPO alternative peptide《Contact regarding PeptiGrowth’s products and application notes》
Contact in North America

MIFI Bioceuticals

Contact in the EU/UK

IVICT Europe GmbH

Hackensack, NJ, USA

Duesseldorf, GERMANY

TEL: 201-242-5104

TEL: +49-(0)172-204-4403

E-mail: PG@mifibioceuticals.com

E-mail: koji.okada@ivic-t.com

PeptiGrowth Inc. Tokyo, JAPAN, TEL : +81-(0)70-4503-1497, E-mail : contact@peptigrowth.com

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