THE TRANSFECTION EXPERTS
Our work at Mirus Bio began with a passion for science. That passion continues today with a brand that reflects ourcommitment to deliver the best results, support and technologies to scientists worldwide. It highlights innovative
products that address the workflow and applications needed in today’s research. Expanding upon our expertise intransfection, Mirus has crafted a more comprehensive offering for nucleic acid delivery. With chemical transfection
reagents, electroporation products and virus production and transduction products, we can now provide the optimaldelivery systems for molecular and cell biology applications.
Highlighted within this brochure is our portfolio of delivery methods that include chemical transfection, electroporationand viral transduction to support relevant cell culture workflows with the best possible experimental results.

MOST RECENT BREAKTHROUGHS
2021: TransIT-VirusGEN® GMP Transfection Reagent and Kits– Large-scale virus production for late-phaseclinical trials and commercial manufacturing
2019: TransIT-VirusGEN® SELECT Transfection Reagent – Large-scale virus production for preclinical andearly phase clinical trials
2019: CHOgro® High Yield Expression System – High titer transient transfection for suspension CHO cells
2018: Ingenio® EZporator® Electroporation System – Easy-to-use and cost-effective electroporation system
for high efficiency transfection of mammalian cells
2017: TransIT-VirusGEN® – Ideal for recombinant adeno-associated virus and lentivirus production
2016: TransIT®-Lenti – Ideal for high titer recombinant lentivirus production
2014: TransIT®-Insect – Optimal insect cell transfection for transgene & baculovirus expression
2013: TransIT-X2® Dynamic Delivery System—Superior delivery of plasmid DNA, siRNA and/or Cas9 RNPOUR PROMISE TO YOU: SERVICE THROUGH EXPERTISE
Order Online 24/7 (Credit Cards or Purchase Orders)
www.mirusbio.com

Customer Service
Hours of Operation: 8:00 AM–4:00 PM Central Time, Monday–Friday
Customer Service Email: sales@mirusbio.com
Customer Service Phone: 888.530.0801 (toll free in the U.S.) or +1.608.441.2852
Fax: +1.608.441.2849

Technical Support
Hours of Operation: 8:30 AM–4:00 PM Central Time, Monday–Friday
Technical Support Email: techsupport@mirusbio.com
Technical Support Hotline: 844.MIRUSBIO (toll free in the U.S.) or +1.608.441.2852

International Distribution

For a complete list of our worldwide
distributors, please visit: www.mirusbio.com
and contact the distributor in your region.

Mail
Mirus Bio LLC
Attention: Customer Service
5602 Research Park Blvd, Ste 210
Madison, WI 53719 USA

Not sure where to start?
START WITH: Reagent Agent®
To determine the best reagent for your experiment,
view citations, customer feedback, and in-housetransfection data with the Reagent Agent®
Transfection Database: www.mirusbio.com/RA

PROVE IT TO YOURSELF: Request a FREE Sample
SAMPLE

Visit: www.mirusbio.com/sample -OREmail: techsupport@mirusbio.com

CONTENTSCHEMICAL TRANSFECTION
Ideal for: Multiple Nucleic Acid Formats Into A Broad Range of Cell Types
TransIT-X2® Dynamic Delivery System................................................................................2-7

Ideal for: Plasmid DNA Transfection

TransIT®-LT1 Transfection Reagent.....................................................................................8-9		
TransIT®-2020 Transfection Reagent..............................................................................10-11

Ideal for: Specific Cell Types

TransIT®-293 Transfection Reagent..................................................................................... 12
TransIT®-BrCa Transfection Reagent .................................................................................. 12
TransIT®-CHO Transfection Kit............................................................................................. 12		TransIT-HeLaMONSTER® Transfection Kit............................................................................. 12
TransIT®-Insect Transfection Reagent................................................................................. 13		TransIT®-Jurkat Transfection Reagent ................................................................................ 13
TransIT®-Keratinocyte Transfection Reagent ....................................................................... 13
TransIT®-Oligonucleotide Transfection Reagent .................................................................. 13

Ideal for: siRNA/miRNATransIT-TKO® Transfection Reagent................................................................................14-15
TransIT-siQUEST® Transfection Reagent.........................................................................14-15

Ideal for: Large RNA (Viral RNA, mRNA & CRISPR/Cas9)

TransIT®-mRNA Transfection Kit.....................................................................................16-17

Ideal for: Insect Cell Transfection & Baculovirus Production
TransIT®-Insect Transfection Reagent............................................................................18-19

Ideal for: Protein & Antibody Production

CHOgro® High Yield Expression System..........................................................................20-22
TransIT-PRO® Transfection Kit........................................................................................23-24

ELECTROPORATION
Ideal for: Plasmid DNA, RNA, siRNA, miRNA and RNP Delivery

NEW! Ingenio® EZporator® Electroporation Kit...................................................................... 25

Ingenio® Electroporation Kit...........................................................................................25-28
Ingenio® Electroporation Solution...................................................................................25-28		
Ingenio® Electroporation Accessories.............................................................................25-28

VIRUS PRODUCTION
Ideal for: Lentivirus and Adeno-associated Virus (AAV) Production

TransIT-VirusGEN® SELECT Transfection Reagent ..........................................................29-32
TransIT-VirusGEN® Transfection Reagent ............................................................................ 33
TransIT®-Lenti Transfection Reagent and the TransIT® Lentivirus System and Lentivirus
Packaging Mix Powered by MISSION®...................................................................34-36

mirusbio.com | Direct +1.608.441.2852

Multiple Nucleic Acid Types Into a Broad Range of Cell Types

TransIT-X2® DYNAMIC DELIVERY SYSTEM• High Efficiency—Exceptional broad spectrum
transfection

PRODUCT NO.

QUANTITYMIR 6003

0.3 ml• Versatile—Cutting edge delivery of plasmidDNA, siRNA/miRNA or ribonucleoprotein (RNP)
complexes

MIR 6004

0.75 ml

MIR 6000

1.5 ml• Technology—Novel, non-liposomal, polymericdeliveryMIR 6005

5 x 1.5 ml

MIR 6006

10 x 1.5 ml"

To inquire about bulk pricing, please call+1.608.441.2852

We recently tested the Trans IT-X2 ® Dynamic Delivery System head-to-head against Lipofectamine ®
2000 for DNA transfection of NIH-3T3 fibroblasts and the breast cancer cell line ZR-75-1. We
observed higher efficiency and less toxicity when using Trans IT-X2 ®. We are also pleased to hearthat Trans IT-X2 ® will be offered in similar volume configurations to Lipofectamine ® 2000.

"

Dr. Edwin Li, Assistant Professor
Saint Joseph’s University

Description
Achieve superior transfections with an innovative polymeric system that efficiently delivers
both DNA and RNA out of the endosome and into the cytoplasm, overcoming a critical barrier
to nucleic acid delivery.
‡ Cell types with >2-fold luciferase
expression in head-to-head comparisons.

TransIT-X2® Dynamic Delivery System
A549‡
AsPC-1
BHK-21‡
BJ‡
BT-20
Caco-2C2C12CFPAC-1‡
CHO-K1‡
DI-TNC1
DU-145
Hep G2‡
HCC1143‡
HCC38
HEK 293
HMEC (epithelial)‡

HUVEC‡
Immortalized Keratinocytes‡
FreeStyleTM 293-F
MDA-MB-468‡
MDCK‡
NHDF‡
AU565
NIH-3T3
BxPC-3
L929‡
COS-7
LNCaP‡HeLa
PC-12
HCT 116
RAW 264.7
MCF-7
T47D‡
MDA-MB-231
PC-3

28

8

Lipofectamine®
2000

5

Keratinocytes‡
MDA-MB-453
Neuro-2a
SH-SY5Y
SK-N-MC‡ Cell types with >2-fold luciferase expression in head-to-head comparisons.

FIGURE 1. The TransIT-X2® Dynamic Delivery System Enables Superior Gene Expression in a Variety of Cell Types.
The TransIT-X2® Dynamic Delivery System (Mirus Bio) and Lipofectamine® 2000 Transfection Reagent (Thermo Fisher
Scientific) were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA
ratios. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Head-tohead comparisons at optimized ratios illustrate superior or equal luciferase expression using TransIT-X2® (Mirus Bio) in
36 of 41 cell types; 17 cell types that had expression levels 2-fold higher are denoted with ‡.

2 Chemical Transfection | mirusbio.com

TransIT-X2® Dynamic Delivery System continued

A549

CHO-K1

Hep G2

PC-12

MDCK

HMEC*

NHDF*

-

FIGURE 2. Visualization of High GFP Expression Using the TransIT-X2® Dynamic Delivery System. The TransIT-X2®
Dynamic Delivery System (Mirus Bio) was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, Hep
G2, LNCaP, MDCK, PC-12, primary human mammary epithelial cells (HMEC) and primary normal human dermal
fibroblasts (NHDF). Transfections were performed in 35 mm dishes (MatTek) using 4-8 μl of TransIT-X2® (Mirus
Bio) to deliver 2 μg of DNA. Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100
inverted fluorescence microscope. *Indicates primary cell types.

100
90

EGFP Positive (%)

80
70
60
50
40
30
20
10
0
A549

CHO-K1 Hep G2

MDCK

LNCaP

PC-12

HMEC*

NHDF*

FIGURE 3. High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells Using the TransIT-X2® Dynamic
Delivery System. The TransIT-X2® Dynamic Delivery System (Mirus Bio) was used to transfect plasmid DNA encoding
EGFP into A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC) andprimary normal human dermal fibroblasts (NHDF). Transfections were performed in 96-well plates using 0.2-0.4 μl
of TransIT-X2® (Mirus Bio) to deliver 0.1 μg of DNA (2:1, 3:1 or 4:1 reagent:DNA ratio). Triplicate wells were assayed
48 hours post-transfection on a guava® easyCyte™ 5HT Flow Cytometer (MilliporeSigma). *Indicates primary cell
types.

CHEMICAL TRANSFECTION

LNCaP

TransIT-X2® Dynamic Delivery System continued
FIGURE 4. Functional Co-delivery of Plasmid DNA andsiRNA Using the TransIT-X2® Dynamic Delivery System.
The TransIT-X2® Dynamic Delivery System (Mirus Bio) wasused to transfect plasmid Cy®5 labeled DNA encoding
nuclear YFP and Cy ®3 labeled siRNA into HeLa cells.
Transfection was performed in a 6-well plate with PolyL-Lysine (PLL) coated coverslips using 4 μl of TransIT-X2®
(Mirus Bio) to deliver 2 μg of DNA (2:1 reagent:DNA ratio)
and 25 nM siRNA. Actin cytoskeleton was stained using
Alexa Fluor ® 350 Phalloidin (Thermo Fisher Scientific).
Image (63X) was captured at 24 hours post-transfection
using a Nikon A1R confocal microscope. Image key: yellow(nuclear YFP), blue (Cy ®5 labeled DNA), red (Cy ®3 labeledsiRNA), green (actin cytoskeleton).

"
"
"
"

We work on non-small cell lung cancer (NSCLC) which is an adherent cell
culture line. Previously, we have tested many transfection products fromseveral companies without much success, but the Trans IT-X2 ® Dynamic
Delivery System works very well with NSCLC using my protocol.
Dr. Luo Wang,
University of Michigan
Comprehensive Cancer Center""

The Trans IT-X2 ® Dynamic Delivery System outperformed all othertransfection reagents we have tested for DNA transfection of our C2C12mouse myoblast cell line. In addition, Trans IT-X2 ® was also less toxic.
Dr. G. Du, Assistant Professor
Texas Medical Center"We are pleased with the performance of the Trans IT-X2 ® Dynamic
Delivery System when transfecting our renal carcinoma cell line
786-0.
Sathish Padi,
North Dakota State University

The Trans IT-X2 ® Dynamic Delivery System performed
better than our regular transfection reagent (Polyjet)for delivering DNA into the hard to transfect A549 cellline. Trans IT-X2 ® was able to show protein expression
compared to Polyjet which failed to produce
detectable levels of protein containing V5 tag.

"

Jason Liggett and Kyung-Won Min, Baek Lab
University of Tennessee

4 Chemical Transfection | mirusbio.com

TransIT-X2® Dynamic Delivery System continued
100
90
80
Knockdown (%)

60
50
40
30
20
10
0

Transfection Reagent
siRNA Target

Lipofectamine® 2000

TransIT-X2®

TransIT-X2®

Lipofectamine® 2000
aha1

GAPDH

FIGURE 5. The TransIT-X2® Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine® 2000. The
TransIT-X2® Dynamic Delivery System (Mirus Bio) and Lipofectamine® 2000 Transfection Reagent (Thermo Fisher
Scientific) were used to transfect siRNA targeting endogenous proteins, GAPDH and aha1, or to deliver a nontargeting control in primary normal human dermal fibroblasts (NHDF). Cells were transfected in a 6-well plate using
4 μl of TransIT-X2® (Mirus Bio) or 6 μl of Lipofectamine® 2000 (Thermo Fisher Scientific) and 25 nM siRNA according
to each manufacturer's protocol. The amount of GAPDH or aha1 mRNA was measured relative to 18S rRNA levels
using qRT-PCR and then scaled to the mRNA levels of the negative control, 48 hours post-transfection. Error bars
represent the standard deviation of triplicate wells.

100

Knockdown of PTK9 mRNA (%)

90
80
70
60
50
40
30
20
10
0
Transfection Reagent
miRNA

TransIT-X2®

Lipofectamine® 2000

Pre-miR™ hsa-miR-1

TransIT-X2®

Lipofectamine® 2000

mirVana™ mimic, miR-1

FIGURE 6. Effective miRNA Delivery Using the TransIT-X2® Dynamic Delivery System Yields Decreased Levels of
PTK9 mRNA. The TransIT-X2® Dynamic Delivery System (Mirus Bio) and Lipofectamine® 2000 Transfection Reagent(Thermo Fisher Scientific) were used to transfect T47D cells with Pre-miR™ hsa-miR-1 miRNA Precursor (Thermo
Fisher Scientific) or mirVana™ miRNA mimic (Thermo Fisher Scientific), miR-1, both known to decrease PTK9 mRNAlevels. A Pre-miR negative control was transfected to assess baseline mRNA levels. Cells were transfected in a 12well plate using 3 μl of TransIT-X2® (Mirus Bio) or Lipofectamine® 2000 (Thermo Fisher Scientific) and 50 nM miRNAaccording to each manufacturer's protocol. The amount of PTK9 mRNA was measured relative to 18S rRNA levels
using qRT-PCR and then scaled to the mRNA levels of the negative control, 48 hours post-transfection. Error bars
represent the standard deviation of triplicate wells.

CHEMICAL TRANSFECTION

70

TransIT-X2® Dynamic Delivery System continued

CRISPR Gene Editing Workflow Using TransIT-X2®
DESIGN• Identify target site
• Design gRNA

TRANSFECTANALYZE• Deliver Cas9 + gRNA

• Enrich• Screen

TransIT-X2 ® Dynamic Delivery System
A novel, polymeric system for deliveryof multiple nucleic acids to mammaliancells. Ideal for delivery of CRISPR/Cas9
components in DNA, gRNA and RNP formats.

Plasmid DNA and Guide RNA Oligonucleotide Transfection
Cas9 protein and guide RNA can both be encoded by plasmid DNA for transfection. Alternatively,
Cas9 can be delivered as plasmid DNA, and guide RNA can be supplied as an RNA oligonucleotide.
Benefits of these approaches include:• Low Cost - Plasmid DNA is a renewable, cost-effective format• Flexibility - Cas9 and guide RNA plasmids are suitable for stable or transient transfection• Ease-of-use - Guide RNA oligonucleotide format enables simple retargeting of Cas9 todifferent loci
FIGURE 7. Efficient Genome Editing with Cas9
Plasmid DNA and Guide RNA Oligonucleotides.
HEK 293T/17, U2OS and NHDF cells were co-transfectedwith 0.5 µg of Cas9 encoding pDNA (MilliporeSigma)
and 50 nM PPIB targeting two-part gRNA (Dharmacon/
GE Healthcare) using The TransIT-X2® Dynamic Delivery
System (2 µl/well of a 24-well plate, Mirus Bio). A
T7E1 mismatch detection assay was used to measurecleavage efficiency at 48 hours post-transfection.

"

I was recently tasked with developing a CRISPR protocol for primary and bone-derived celllines. Trans IT-X2 ® was simple to use, 2-3 times better for transfection and much gentler
on my cells than other products! I feel I have hit the jackpot and have already passed thisexciting information on to my colleagues.
Joshua Chou, Ph.D.
Harvard School of Dental Medicine"

6 Chemical Transfection | mirusbio.com

TransIT-X2® Dynamic Delivery System continued

Cas9/gRNA Ribonucleoprotein (RNP) Transfection•••

High Efficiency Delivery - Deliver Cas9/gRNA complexes to multiple cell types,
including hard to transfect cells such as immune and stem cells
High Specificity - Pre-formed RNP complexes provide a rapid pulse of genome editing
activity
DNA Free - No risk of insertional mutagenesis

FIGURE 8. Trans IT-X2 ® Outperforms
Other Reagents for RNP Delivery.
Ribonucleoprotein(RNP)
complexes
composed of PPIB (cyclophilin B) targeting
2-part gRNA (IDT) and Cas9 protein (PNA
Bio) were delivered into (A) HEK 293T/17
and (B) U2OS cells using TransIT-X2®
Dynamic Delivery System (1 µl/well, Mirus
Bio) or Lipofectamine® CRISPRMAX™ (1.5µl/well and 1 µl/well of Lipofectamine®
Cas9 Plus™ Reagent, Thermo Fisher) or
Lipofectamine® RNAiMAX (1.5 µl/well,
Thermo Fisher) or Lipofectamine® 3000
(1.5 µl/well and 1 µl/well of P3000™
Reagent, Thermo Fisher) in a 24-wellformat according to the manufacturers’
protocol. Varying levels of gRNA (6 nM or
12 nM) were tested with 6 nM Cas9 protein(PNA Bio). A T7E1 mismatch detection
assay was used to measure cleavage
efficiency at 48 hours post-transfection.

A.

293T/17

Transfec�on Reagent:
Cas9 protein (nM):gRNA (nM):TransIT®-X2
6
6
12

CRISPRMAX™
6
6
12

61

11

6

RNAiMAX™
6
12

Lipofectamine® 3000
6612RNAiMAX™
6
12

Lipofectamine® 3000
6
6
12

uncut

cut

Cleavage eﬃciency (%)

66

B.

9

U2OS

Transfec�on Reagent:
Cas9 protein (nM):gRNA (nM):TransIT®-X2
6
6
12

CRISPRMAX™
6
6
12

53

14

6

uncut

cut

Cleavage eﬃciency (%)

62

8

FIGURE 9. Genome Editing in IPS Cells with Cas9 and Guide RNA
Ribonucleoprotein Complexes. The TransIT-X2® Dynamic Delivery
System was used to deliver Cas9 protein/guide RNA ribonucleoprotein(RNP) complexes in human induced pluripotent stem cells (iPSCs). A T7E1
mismatch assay was used to measure cleavage efficiency at 48 hours
post-transfection.

For more on CRISPR/Cas9 delivery, please see
Page 17 for gRNA ribonucleoprotein delivery with TransIT® -mRNA andPage 26 for RNP delivery with Ingenio ® Electroporation Solution.

CHEMICAL TRANSFECTION

Purified Cas9 protein can be combined with guide RNA to form an RNP complex to be delivered
to cells for rapid and highly efficient genome editing. Benefits of RNP-based genome editing
include:Plasmid DNA Transfection

TransIT®-LT1 TRANSFECTION REAGENT• Broad Spectrum DNA Delivery—Utilize one
transfection reagent and protocol for a varietyof cells• Low Cellular Toxicity—Maintain cell density
and reduce experimental biases• Deliver Single or Multiple Plasmids—
Suitable for many applications such as gene
expression, shRNA expression, virus production
and promoter analysis

PRODUCT NO.

QUANTITYMIR 2304

0.4 mlMIR 2300

1.0 mlMIR 2305

5 x 1.0 ml

MIR 2306

10 x 1.0 ml

To inquire about bulk pricing, please call+1.608.441.2852"

We routinely use Mirus Trans IT ® -LT1 Transfection Reagent for the delivery ofplasmid DNA to carry out immunoprecipitation experiments. Our lab recently
published using Trans IT ® -LT1 for this application to reveal a crucial
regulator (MCUR1) for calcium uptake in the mitochondria to regulate
cellular metabolism.” (Mallilankaraman, K et al. Nature Cell Biology. December2012).

"

Dr. Karthik Mallilankaraman,
Madesh Laboratory, Center for Translational Medicine, Temple University

Description
The TransIT®-LT1 (Low Toxicity) Reagent is a broad spectrum, high efficiency DNA transfection
reagent that is easy to use and exhibits minimal cellular toxicity. This reagent is a proprietary
formulation of polyamines and cationic lipids that efficiently transfects cells in the presenceof serum.
2,500,000

40
35

2,000,000

25

1,500,000

20
1,000,000

15

Cytotoxicity (%)

Luciferase (RLU)

30

10500,000

5
0

0
2:1
Cells
Alone

4:1
3:1
TransIT®-LT1

1.5:1

3:1 5:1 1.5:1 3:1:1 5:1:1 1.5:1 2:5:1 3:5:1
FuGENE® HD
Lipofectamine® 2000 Lipofectamine® LTX
and PLUS™

FIGURE 10. TransIT®-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other
Transfection Reagents. Hep G2 cells were transfected with a luciferase expression plasmid using the designatedreagents at the manufacturers' recommended reagent-to-DNA ratio indicated beneath each bar. Luciferase
expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours posttransfection. LDH levels are reported as percent cytotoxicity compared to cells alone. Experiments were performed
as per industry accepted testing protocols. FuGENE is a registered trademark of Fugent LLC.

8 Chemical Transfection | mirusbio.com

100
80
60
40
20

A5
49
CH
OK1
CO
SH
EK 7
29
3
H
eL
a
H
Ke ep
G
ra
tin 2
oc
yt
es
LN
Ca
N
eu P
ro
-2
a
N
IH
3T
3
PC
-3

0

FIGURE 11. The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using theTransIT®-LT1 Transfection Reagent (Mirus Bio), cells were transfected with the pEGFP-C1 expression vector, and thepercentage of EGFP expressing cells was determined 24-48 hours post-transfection by flow cytometry.

Luciferase Activity (RLU)

1.0E + 08

1.0E + 07

1.0E + 06

1.0E + 05
CHO-K1

COS-7

HEK 293®

TransIT -LT1

HeLa®

FuGENE 6

FIGURE 12. Comparable Luciferase Expression With TransIT®-LT1 Reagent and FuGENE® 6 in Multiple Cell Types.
The indicated cell lines were transfected in duplicate with 1 µg of a luciferase expression vector per well of a 12well plate using either 3 µl of TransIT®-LT1 (Mirus Bio) or FuGENE® 6 Reagents (Fugent LLC) according to industry
accepted testing protocols. Cells were harvested 24 hours post-transfection and assayed for luciferase activity.
FuGENE is a registered trademark of Fugent LLC.
FIGURE 13. Exceptional Transfection Efficiency in Human
Induced Pluripotent Stem Cells (iPSCs) via Reverse
Transfection with the TransIT®-LT1 Transfection Reagent.
The TransIT®-LT1 Transfection Reagent (Mirus Bio) wasused to reverse transfect 1.3 x 106 iPSCs with a ZsGreen
expressing plasmid (Clontech). Cells were visualized 48
hours post-transfection.

Data courtesy of Cellular Dynamics International (CDI), a FUJIFILM Company.

CHEMICAL TRANSFECTION

EGFP Expressing Cells (%)

TransIT®-LT1 Transfection Reagent continued

Plasmid DNA Transfection

TransIT®-2020 TRANSFECTION REAGENT• Broad Spectrum DNA Delivery—Achieve highexpression in many cell types, including hardto-transfect and primary cells• Outperforms Competitor Reagents—
TransIT®-2020 demonstrates higher protein
yield and less toxicity when compared to othertransfection reagents• Animal Origin Free—provides highperformance with maximum compatibility

PRODUCT NO.

QUANTITYMIR 5404

0.4 mlMIR 5400

1.0 mlMIR 5405

5 x 1.0 ml

MIR 5406

10 x 1.0 ml

To inquire about bulk pricing, please call+1.608.441.2852"

Using Trans IT ® -2020, we transfected HeLa cells in 6-well plates with 1.25
µg of the Zhang lab construct (pX330) from Addgene that harbors both a
specific guide RNA against a recognition sequence in our gene of choice,and 1.25 µg of a donor plasmid with 1 kb of 5’ and 3’ homology sequence.
We then selected the cells using puromycin and came across a populationthat harbored the modification we were interested in. Thank you so muchfor the sample of TransIT®-2020. Mirus has always been without exception
the gold standard for me and why anyone else would want to use anythingelse is just beyond me.

"

Aviva Joseph,
University of Massachusetts Medical School

Description
TransIT-2020® Reagent is a versatile transfection solution for broad spectrum DNA delivery
into mammalian cells. This reagent is animal component free allowing maximum compatibility
for all downstream applications while outperforming other reagents in many cell types.
FIGURE 14. High Performance Plasmid Transfection.
Primary Human Small Epithelial cells (HSAEpC) weretransfected using TransIT®-2020 (Mirus Bio) and an EGFP
expression plasmid (4:1 reagent-to-DNA ratio). Images
were taken 24 hours post-transfection using an inverted
fluorescence microscope (Zeiss Axiovert).

"

I recently tested Trans IT ® -2020 and Trans IT ® -LT1, and both reagents
worked well in terms of their efficiency at transfecting human-derived iPS
cells with CRISPR constructs and a fluorescent protein reporter. Throughvisual inspection, transfection efficiencies with Trans IT ® -2020 andTrans IT ® -LT1 were clearly higher than with Lipofectamine ® 3000.
Fedir Kiskin,
University of Cambridge

10 Chemical Transfection | mirusbio.com"

TransIT®-2020 Transfection Reagent continued

90
80
70
60
50
40
30
20
10
0
HUVEC

THP-1

TransIT®-2020

NT2

CHO-K1FuGENE® HD

HEK-293

Lipofectamine® 2000

FIGURE 15. Superior Gene Expression in a Broad Spectrum of Cell Types. The indicated cell types were transfected
in 96-well plates with a luciferase expression plasmid (0.1 μg/well) according to industry accepted testing
protocols. Reagent-to-DNA ratios were optimized for each cell type: TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE®
HD (Promega, 3.5:1), Lipofectamine® 2000 (Thermo Fisher Scientific, 1.5:1, 3:1 or 5:1). Luciferase activity wasmeasured 24 hours post-transfection. Values were normalized to TransIT®-2020 and presented as a percentage of
luciferase expression. FuGENE is a registered trademark of Fugent LLC.

900,000

70
60

700,000

50

600,000
500,000

40

400,000

30

300,000

20

200,000

10

100,000
0

Toxicity (%)

Luciferase (RLU)

800,000

2:1

3:1

4:1

TransIT®-2020

1.5:1 3:1

5:1

1.5:1:1 3:1:1 5:1:1

Lipofectamine®
2000

Lipofectamine®
LTX
and PLUSTM

0

FIGURE 16. TransIT®-2020 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other
Transfection Reagents. Human Umbilical Vein Endothelial Cells (HUVEC) were transfected with a luciferase
expression plasmid using the designated reagents at the indicated reagent-to-DNA ratios. Transfections were
performed in 96-well plates. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph)
were measured at 24 hours post-transfection. LDH levels are reported as percent cytotoxicity compared to cells
alone. Error bars represent the standard deviation of triplicate wells.

CHEMICAL TRANSFECTION

Normalized Luciferase Expression (%)

100

Cell Type Specific

TransIT® CELL TYPE SPECIFIC TRANSFECTION REAGENTS
TransIT® Cell Line Specific DNA Transfection Reagents are formulated to maximize transfection
efficiency while maintaining cellular health in many popular or hard-to-transfect cell types.
All of these reagents offer:• Optimized Formulations—Designed for each cell type
• Low Cellular Toxicity—Maintain cell density and reduce experimental biases due totoxicity-induced cellular changes• Serum Compatible—No media changes necessary or extensive optimization required,saving valuable research time
Applicable Cell Line(s)or Cell Type(s)Product*

Efficiency**

Product No. Quantity

TransIT®-293 Transfection Reagent
HEK 293,
HEK 293T,
and related

75–85%MIR 2704

0.4 mlMIR 2700

1.0 mlMIR 2705

5 x 1.0 ml

MIR 2706

10 x 1.0 ml

MIR 5504

0.4 mlMIR 5500
MIR 5505

1.0 ml5 x 1.0 ml

MIR 5506

10 x 1.0 ml

TransIT -BrCa Transfection Reagent®

MCF-7, MDA-MB-231, MDAMB-453, MDA-MB-468,
T47D

40–80%

TransIT®-CHO Transfection Kit (TransIT®-CHO Reagent & CHO Mojo Reagent)

50–60%

CHO-K1 and related

MIR 2174

0.4 mlMIR 2170
MIR 2175

1.0 ml5 x 1.0 ml

MIR 2176

10 x 1.0 ml

TransIT-HeLaMONSTER Transfection Kit (TransIT -HeLa Reagent and MONSTER Reagent)®

®

"

HeLa and related

50–60%MIR 2904

0.4 mlMIR 2900
MIR 2905

1.0 ml5 x 1.0 ml

MIR 2906

10 x 1.0 ml

Our lab has been satisfied with the routine use of the Trans IT-HelaMONSTER ®
Transfection Kit. Transfections exhibit high target protein expression withvery little cell toxicity. Cells remain viable post-transfection and can be readily
infected with virus without any problems.

"

Dr. Corine St. Gelais,
The Ohio State University − Center for Retrovirus Research

12 Chemical Transfection | mirusbio.com

TransIT® Cell Type Specific Transfection Reagents continued

Product*

Applicable Cell Line(s)or Cell Type(s)Efficiency**

Product No. Quantity–

High Five™, S2, Sf9

MIR 6104

0.4 mlMIR 6100
MIR 6105

1.0 ml5 x 1.0 ml

MIR 6106

10 x 1.0 ml

MIR 2124

0.4 mlMIR 2120
MIR 2125

1.0 ml5 x 1.0 ml

MIR 2126

10 x 1.0 ml

MIR 2804
MIR 2800
MIR 2805

0.4 ml
1.0 ml5 x 1.0 ml

MIR 2806

10 x 1.0 ml

TransIT -Jurkat Transfection Reagent®

Jurkat, Jurkat-E6, RAW
264.7, THP-1, K562, andother lymphoid cell lines

5-10%

TransIT -Keratinocyte Transfection Reagent®

Immortalized Keratinocyte

20–30%

To inquire about bulk pricing, please call +1.608.441.2852*

Single tube reagents contain the indicated transfection reagent. Transfection reagents with two components are named "Kits" and both components arelisted following the product name.

** Transfection efficiency determined by transfection of an EGFP expression vector followed by visual quantification of the percentage of cells expressing EGFP or via
flow cytometry.

Oligonucleotide

TransIT® REAGENTS FOR OLIGONUCLEOTIDE DELIVERY• Unique Formulation—Maximize DNA and RNA
oligonucleotide transfection efficiency in a wide
range of cells
Oligonucleotides Tested
phosphodiester DNA, phosphothioate DNA (sDNA),
phosphothioate RNA (sRNA), 2'OMe RNA, 2'OMe
RNA/sDNA Chimerics, and Morpholino/DNA
duplexes.

PRODUCT NO.

QUANTITYMIR 2164

0.4 mlMIR 2160

1.0 mlMIR 2165

5 x 1.0 ml

MIR 2166

10 x 1.0 ml

To inquire about bulk pricing, please call+1.608.441.2852

FIGURE 17. TransIT®-Oligo Reagent Achieves High
Transfection Efficiency. HeLa cells were transfectedwith Cy ®3 and fluorescein labeled phosphothioate
DNA oligos using TransIT®-Oligo Reagent and observed
24 hours post-transfection. The yellow punctate
fluorescence is the result of co-localization of thefluorescein and Cy ®3 labeled oligonucleotides after
transfection. The image was acquired using a confocal
microscope.

CHEMICAL TRANSFECTION

TransIT®-Insect Transfection Reagent

siRNA/miRNATransIT-TKO® & TransIT-siQUEST®
TRANSFECTION REAGENTS
TransIT-TKO® Transfection Reagent• High Knockdown Efficiency—Achieve
optimal gene silencing in a large percentage
of cells to ensure experimental success• Low Cellular Toxicity—Maintain cell density
and reduce experimental biases due toalterations in cellular health• Flexible Protocol—use with either standard
or reverse transfections

PRODUCT NO.		

QUANTITYMIR 2154		

0.4 mlMIR 2150		

1.5 mlMIR 2155		

5 x 1.5 ml

MIR 2156		

10 x 1.5 ml

TransIT-siQUEST® Transfection Reagent"

We have tried other transfection reagents, but
only the Trans IT-TKO ® reagent gives us a 100%transfection rate and gene knockdown without
toxicity in these cells (RAW 264.7).

"

Nature Protocols, 1: 508 - 517 (2006)

PRODUCT NO.		

QUANTITYMIR 2114		

0.4 mlMIR 2110		

1.5 mlMIR 2115		

5 x 1.5 ml

MIR 2116		

10 x 1.5 ml

To inquire about bulk pricing, please call+1.608.441.2852

Description
TransIT-TKO® and TransIT-siQUEST® small interfering RNA (siRNA and miRNA) Transfection
Reagents are broad spectrum reagents that are easy to use and exhibit minimal cellular toxicity.
Each reagent is uniquely formulated and exhibits distinct siRNA/miRNA transfection profiles.
Testing these two reagents allows users to identify a formulation which is optimal for achieving
knockdown in their particular cell line.
100

Luciferase Expression (%)

TransIT-siQUEST® Reagent
TransIT-TKO® Reagent

40

Lipofectamine® 2000

30

20

10

ro
Ve

7
26

4.

3
3T
IH

W
RA

-7
N

CF
M

G2

29

ep

3
H

7
S-

H
EK

CO

Re
a
Al gen
on t
e
CH
OK1

0

FIGURE 18. Knockdown Efficiencies Using TransIT-siQUEST®, TransIT-TKO® Reagents and Lipofectamine® 2000.
Firefly and sea pansy luciferase reporter vectors were co-transfected into various cell lines using the TransIT®LT1 Reagent (Mirus Bio). Subsequently, firefly luciferase expression was knocked down by transfection of 25 nM
anti-firefly luciferase siRNA using either TransIT-siQUEST® (red, (Mirus Bio)), TransIT-TKO® (tan, (Mirus Bio)) or
Lipofectamine® 2000 (gray, Thermo Fisher Scientific) Reagents. Bars indicate the percent of normalized firefly
luciferase expression as compared to each reagent alone control 24 hours post-transfection.

14 Chemical Transfection | mirusbio.com

TransIT-TKO® & TransIT-siQUEST® Transfection Reagents continued

TransIT-TKO® TransIT-siQUEST®
Knockdown
Knockdown
Efficiency
Efficiency

Luciferase*
MAPK1
MAPK3
Luciferase*
Luciferase*
Lamin A/C
HeLa (human cervix)
GAPDH
HeLa-luc (human cervix)
Luciferase*
Hepa-luc (mouse liver)
Luciferase*
HepG2 (human liver)
MAPK1
NIH 3T3-luc (mouse fibroblast) Luciferase*
MAPK1
NIH 3T3-L1
MAPK3
Secondary Human Astrocytes
Lamin A/C
ABC A1Lamin A/C
Primary Mouse Hepatocytes
PPAR-alphaNormalized GAPDH Expression (%)

A549-luc (human lung)
BNL CL.2(mouse liver)CHO-luc (hamster ovary)
HEK 293-luc (human kidney)

77%
80%
83%
86%
83%
80%
80%
84%
-80%
85%
70%
70%
80%
70%
81%
--

82%
--91%
77%
--82%
92%
-89%
-----82%

TABLE 1. Knockdown of Genes Using
TransIT‑TKO® or TransIT‑siQUEST®
Transfection Reagents. Cells weretransfected with siRNAs targeting
the indicated genes using theTransIT‑TKO® or TransIT‑siQUEST®
Reagents (Mirus Bio), and theknockdown
percentage
wasdetermined using quantitative RTPCR or luciferase assays.
*Firefly luciferase expression
vectors were stably integrated
into the parent cell lines andclonal lines constitutively
expressing firefly luciferase were
used.

100
80
60
40
20
0

qPCR Probe

GAPDH

GAPDH

GAPDH

GAPDH

GAPDH

GAPDH

siRNA Target

50nM Control
siRNA

50nM
siRNA

50nM Control
siRNA

50nM
siRNA

50nM Control
siRNA

50nM
siRNA

Transfection Reagent

TransIT-TKO® 3μl

TransIT-TKO® 4μl

TransIT-TKO® 5μl

FIGURE 19. High Efficiency Endogenous Knockdown in iCell® Cardiomyocytes. The TransIT-TKO® Transfection
Reagent (Mirus Bio) was used to transfect iCell® Cardiomyocytes (Cellular Dynamics International (CDI), a FUJIFILM
Company) plated at a density of 136,500 cells per well of a 12-well plate pre-coated with fibronectin. Seven days
post-plating, triplicate wells were transfected with TransIT-TKO® (3-5 µl per well, Mirus Bio) and non-targeting
control siRNA or GAPDH targeting siRNA (50 nM per well). Seventy-two hours post-transfection, the amount of
GAPDH mRNA was measured relative to 18S rRNA levels using qRT-PCR and then scaled to the expression level ofthe non-targeting control siRNA. Error bars represent the standard error of the mean (SEM) of three independent
complexes.

CHEMICAL TRANSFECTION

Endogenous
Transcript

Cell Line (Source)Large RNA (Viral RNA and mRNA)

TransIT®-mRNA TRANSFECTION KIT• High Efficiency Delivery—Ensures experimental
success by effectively transfecting RNA into alarge percentage of the cell population• Low Cellular Toxicity—Maintain cell density andreduce transfection induced toxicity• Serum Compatible— Perform transfections in thepresence of serum which eliminates the need for a
media change and maintains cellular health

PRODUCT NO.		

QUANTITYMIR 2225		

0.4 mlMIR 2250		

1.0 mlMIR 2255		

5 x 1 ml

MIR 2256		

10 x 1 ml

To inquire about bulk pricing, please call+1.608.441.2852• Deliver Various Sizes of RNA—Ideal
for specialized applications, such as viralproduction, protein expression from mRNAand stem cell reprogramming
Description
The TransIT®-mRNA Transfection Kit provides high efficiency transfection of large RNA
molecules such as mRNA or viral RNA. The kit is easy to use and minimizes cellular toxicity due
to its ability to transfect RNA in the presence of serum.

4,000% EGFP Positive
Cell Count

75

3,000

50

2,000

25

1,000

Cell Count (viability)

EGFP Positive Cells (%)

100

0

0
TransIT®-mRNA
1:1:1

RNAiMax™
4:1

Stemfect™
2:1

FIGURE 20. High Efficiency and Low Toxicity Transfection Following 14 Consecutive Transfections With the TransIT®mRNA Transfection Kit. Repeated daily transfections were performed in the same population of BJ fibroblasts using
three commercially available transfection reagents – the TransIT®-mRNA Transfection Kit (Mirus Bio), Lipofectamine®
RNAiMAX (Thermo Fisher Scientific) and Stemfect™ RNA Transfection Kit (Stemgent) - with a capped andpolyadenylated EGFP mRNA incorporating pseudouridine and 5mC modified bases (Trilink Biotechnologies). Multiple
reagent-to-RNA ratios were tested and the optimal ratio is represented. Transfections were performed in 12-wellplates using the indicated reagent-to-RNA ratios to deliver 1 µg of RNA. Transfection efficiency was measured
by flow cytometry on a guava® easyCyte™ 5HT Flow Cytometer (MilliporeSigma) following 14 consecutive dailytransfections (blue bars). Cell viability was determined using cell counts measured during flow cytometry (grey bars).
Error bars represent the standard deviation of triplicate wells.

16 Chemical Transfection | mirusbio.com

TransIT®-mRNA Transfection Kit continued

Please see pages 6-7 for CRISPR/Cas9 delivery with TransIT-X2 ® andpage 25 for Ingenio ® Electroporation Solution.
90. 80
GFP+ (%)

70
60
50
40
30

DC
BM
ne
uri
M
ary
im
Pr

e
Lin
ell
IC
SI
W
JA

No RNA Control

ine
ll L
Ce
2.4
C
D

FIGURE 22. Multiple Dendritic Cell Types Express GFP frommRNA Transfected Using the TransIT®-mRNA Transfection
Kit. Murine primary bone marrow derived dendritic cells(BMDC) and murine dendritic cells (JAWS II and DC 2.4)
were transfected with 1 μg of capped and polyadenlyatedmRNA encoding GFP using a TransIT®-mRNA Reagent(Mirus Bio): Boost: mRNA ratio of 1:1:1 (μl:μl:μg). All cells
were seeded (80,000 cells/well) overnight in 24-wellplates. Cells were assayed via flow cytometry 8 hours posttransfection. Error bars represent the standard deviation ofat least 3 separate experiments.
Data courtesy of Kyle Phua
(Principal Investigator: Kam W. Leong), Duke University.

MHV RNA Transfected

FIGURE 23. The TransIT -mRNA Transfection Kit Successfully Delivers Viral RNAs 32 kb Long. A 32 kb in vitrotranscribed RNA of the murine coronavirus, MHV, was transfected into DBT cells using the TransIT®-mRNA
Transfection Kit (Mirus Bio). Successful transfection assessed by the formation of syncytia 24-48 hours posttransfection. Syncytia were visualized by phase contrast microscopy.
Data courtesy of Mark Clemenz, Loyola University of Chicago.
®

CHEMICAL TRANSFECTION

FIGURE 21. Efficient Genome Editing with Cas9 mRNA and Guide RNA Oligonucleotides. HEK 293T/17, U2OS and
NHDF cells were co-transfected with 0.5 µg of Cas9 encoding mRNA, 5mC, (Trilink Biotechnologies) and 25 nM of
PPIB targeting two-part gRNA (Dharmacon/GE Healthcare) using TransIT®-mRNA Transfection Kit (0.5 µl/well of
24-well plate of both mRNA reagent and boost, Mirus Bio). A T7E1 mismatch detection assay was used to measurecleavage efficiency at 48 hours post-transfection.

Insect Cell Transfection and Baculovirus Production

TransIT®-INSECT TRANSFECTION REAGENT• Exceptional DNA Delivery—In insect cell
types including Sf9, High Five™ and S2PRODUCT NO.

QUANTITYMIR 6104

0.4 ml• High Baculovirus Production—Ideal forbaculovirus expression in insect cells

MIR 6100

1.0 ml• Serum Compatibility—Non-liposomal,
animal-origin free formulation that eliminates
the need for media change

MIR 6105

5 x 1.0 ml

MIR 6106

10 x 1.0 ml• Better Value—Less reagent required pertransfection

To inquire about bulk pricing, please call+1.608.441.2852

Description
Insect cell expression is a platform used to produce proteins with simple post-translational
modifications. Transient transfection and recombinant baculovirus production are commonlyused methods for insect cell expression. The TransIT®-Insect Transfection Reagent is an animal
origin free transfection reagent specifically optimized for high gene expression in a variety of
insect cell types.
A.

B.

Tra

Ce

lls

alo

ne
ns
IT ®
-In
Es
se
co
rt™ ct (2
:1)
IV
Ce
(8
llf
:
1
ec
tin ® )II (6:1)

FIGURE 24. Efficient Transfection of Baculovirus Genomic DNA Using The TransIT®-Insect Reagent. Transfections
were performed in 6-well plates with 5 x 105 Sf9 cells per well using the TransIT®-Insect Transfection Reagent(Mirus Bio) at the reagent-to-total DNA ratio of 3:1 (µl:µg). Cells were co-transfected with 0.5 µg of ProGreen™ (AB
Vector) baculovirus genomic vector DNA (AB Vector) encoding green-fluorescent protein (GFP) and 0.1 µg of pVL1393
transfer vector (AB Vector). (A) Fluorescence and phase contrast images were taken at 6 days post-transfection
using a Zeiss S100 fluorescent microscope. Merge shown in (B).

250
150
100
75
50

*

37
25
20
G-250

18 Chemical Transfection | mirusbio.com

FIGURE 25. Superior Recombinant Protein Expression in HighFive™ Cells Using TransIT®-Insect. High Five™ cells (Thermo
Fisher Scientific) were transfected in 6-well plates with 2.5 µg of
a GFP expression plasmid driven by an hr5 enhancer/IE1 promoter
using the designated reagent at the indicated reagent-to-DNA
ratios (µl:µg). Total soluble cell lysates were prepared from cells
72 hours post-transfection. Lysates from 100 µl culture wereanalyzed by SDS-PAGE and Coomassie blue staining; cells alone(untransfected) is shown as control. The highest level of expressionof GFP-6XHis-S-HSV (*, ~38 kDa) was detected in lysate from thecells that were transfected with TransIT®-Insect (Mirus Bio).

TransIT®-Insect Transfection Reagent continued
14,000,000,000

30,000,000,000

Sf9

12,000,000,000

20,000,000,000

Luciferase (RLU)

8,000,000,000
6,000,000,000
4,000,000,000

15,000,000,000
10,000,000,000
5,000,000,000

2,000,000,000
0

0
2:1

3:1

4:1

6:1

TransIT®-Insect

8:1

10:1

4:1

Cellfectin® II

6:1

2:1

8:1

ESCORT™ IV

3:1

4:1

6:1

8:1
Cellfectin® II

TransIT®-Insect

10:1

4:1

6:1

8:1

ESCORT™ IV

6,000,000,000

S2

Luciferase (RLU)

5,000,000,000
4,000,000,000
3,000,000,000
2,000,000,000
1,000,000,000
0
2:1

3:1

TransIT®-Insect

4:1

6:1

8:1
Cellfectin® II

10:1

4:1

6:1

8:1

ESCORT™ IV

FIGURE 26. TransIT®-Insect Outperforms Competitor Transfection Reagents. Insect cell lines Sf9, High Five™
(Thermo Fisher Scientific) and Drosophila S2 cells were transfected in 96-well plates with 0.1 µg of a luciferase
expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagentto-DNA ratios (µl:µg). Luciferase expression was measured at 48 hours post-transfection. Sf9 and High Five™
(Thermo Fisher Scientific) cells were cultured and transfected in serum-free media formulations; S2 cells were inserum containing medium. Error bars represent the standard error of the mean for triplicate wells.
35,000,000,000
24 hr
48 hr

30,000,000,000

72 hr

Luciferase (RLU)

25,000,000,000
20,000,000,000
15,000,000,000
10,000,000,000
5,000,000,000
0
Sf9

High Five™

S2

FIGURE 27. TransIT -Insect Yields Increased Protein Expression Over Time. Insect cell lines Sf9, High Five™
(Thermo Fisher Scientific) and Drosophila S2 were transfected in a 96-well plate with 0.1 µg of a luciferase
expression plasmid driven by an hr5 enhancer/IE1 promoter using the TransIT®-Insect Transfection Reagent (Mirus
Bio) at a reagent-to-DNA ratio of 2:1 (µl:µg). Luciferase expression was measured at three time points: 24, 48 and72 hours post-transfection. Sf9 and High Five™ (Thermo Fisher Scientific) cells were cultured and transfected inserum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard errorof the mean for triplicate wells.
®

"

Our lab successfully tested Trans IT ® -Insect Transfection Reagent for
generating recombinant baculovirus in insect cells. Using Trans IT ® -Insectwith multiple BEVS we were able to generate high-titer baculovirus that
resulted in consistently higher protein expression in High Five™ and Sf9
cells compared to Cellfectin ® II (Thermo Fisher Scientific).” (Kuo et al, Protein
Eng Des Sel. Oct 2012).

"

Dr. Linda Lua (Director), Protein Expression Facility
The University of Queensland

CHEMICAL TRANSFECTION

Luciferase (RLU)

High FiveTM

25,000,000,000

10,000,000,000

The Most Advanced System for Transient Transfection and Protein Production in Suspension CHO Cells

CHOgro® HIGH YIELD EXPRESSION SYSTEM• Efficient—Enables high protein titers withsimple workflow• Convenient—Quick adaptation to CHO celllineages• Optimized—High density growth with minimal
cell clumping post transfection• Worry-free—No commercial license required;animal origin freeDescription

The NEW CHOgro® High Yield Expression System is themost advanced and cost-effective transient transfection
system for high-yield protein production in suspension
CHO cells. Our second-generation system features the
CHOgro® Titer Enhancer, which provides rapid, industryleading protein yields.
•••

High Yield - Reach higher antibody titers in sevendays - faster than the ExpiCHO™ Expression System
Simple Workflow - Same-day transfection,enhancer addition and temperature shift
Worry Free - No commercial license required;animal origin freeComplete CHOgro® Expression System(System includes: CHOgro® Expression Media,
TransIT-PRO® Transfection Reagent, CHOgro®
Complex Formation Solution, CHOgro® Enhancer and
L-Glutamine Solution)

PRODUCT NO.

QUANTITYMIR 6270

1 System

Individual Components,
Available Separately
PRODUCTPRODUCT NO. QUANTITY

CHOgro® Transfection and Titer
Enhancer Kit(Kit includes: TransIT-PRO® Transfection Reagent,
1 ml, CHOgro® Enhancer, 20 ml)

MIR 6225

1 Kit

CHOgro Expression Medium
MIR 6200 1 Liter®

Liquid Polybag
CHOgro® Expression Medium
MIR 6202 10 Liters
Dry Powder
CHOgro® Expression Medium
MIR 6201 Prepares		
10 Liters
TransIT-PRO® Transfection Reagent
MIR 5740 1 ml
MIR 5750 10 ml
CHOgro® Complex Formation Solution
MIR 6210 100 ml

Complete CHOgro High Yield Expression System®

Poloxamer 188 Solution
MIR 6230

100 ml

L-Glutamine Solution
MIR 6240

100 mlAccessory, Sold Separately
Not Included with Kit
PRODUCTPRODUCT NO. QUANTITY

Human IgG1 Expression Control
MIR 6250 100 µg
To inquire about bulk pricing, please call+1.608.441.2852

20 Chemical Transfection | mirusbio.com

CHOgro® High Yield Expression System continued

Internal Control

800
700

60
40

Day 7

Day 140

Day 7 Day 14

CHOgro® High Yield

ExpiCHO

Cetuximab

Day 7 Day 14

CHOgro® High Yield

ExpiCHO0

Rituximab

700

700

500

IgG (mg/L)

IgG (mg/L)
Day 7 Day 14

ExpiCHO

400
300
200

0

Day 7 Day 14

Day 7 Day 14

CHOgro® High Yield

ExpiCHO

Trastuzumab

600
500
400
300
200
100

100
Day 7 Day 14

150
50

Day 7 Day 14

600

CHOgro® High Yield

250
200
100

20

100

IgG (mg/L)

350
300

80

IgG (mg/L)

IgG (mg/L)

IgG (mg/L)

300
200

Bevacizumab

Day 7 Day 14

Day 7 Day 14

CHOgro® High Yield

ExpiCHO0

Day 7 Day 14

Day 7 Day 14

CHOgro® High Yield

ExpiCHO

FIGURE 28. The CHOgro® High Yield Expression System Outperforms the ExpiCHO Expression System Using Multiple
Antibody Constructs. Six different IgG1 antibody constructs, including five therapeutically relevant constructs
synthesized in the same vector backbone were produced by transient transfection using either the TransIT-PRO®
Transfection Reagent (Mirus Bio) at a 1:1 reagent-to-DNA ratio (vol:wt) and 1 µg plasmid DNA per milliliter of culture
in FreeStyle™ CHO-S cells (Thermo Fisher Scientific) cultured in CHOgro® Expression Medium (Mirus Bio) at a celldensity of 4 x 106 cells/ml, or the Expifectamine CHO Transfection Kit (Thermo Fisher Scientific) at a 3.2:1 reagentto-DNA (vol:wt) and 1 µg plasmid DNA per milliliter of culture in ExpiCHO-S cells (Thermo Fisher Scientific) cultured
in ExpiCHO™ Expression Medium (Thermo Fisher Scientific) at a cell density of 6 x 106 cells/ml. For complete
experimental details, please visit: www.mirusbio.com/products/transfection/chogro-high-yield-expression-system.

250

800

200

600

30
25

Titer (mg/L)

1000

Titer (mg/L)

Titer (mg/L)

CHO-S Cells in CHOgro® Expression Medium
ExpiCHO™ Cells in CHOgro® Expression Medium

150

20
15

100

400

10
50

200

0

IgG1 Internal Control

0

5

0

Bevacizumab

Fc construct 1

FIGURE 29. FreeStyle™ CHO-S and ExpiCHO Cell Grown in CHOgro Expression Medium Yield Similar Titers.
FreeStyle™ CHO-S cells (Thermo Fisher Scientific) or ExpiCHO-S cells (Thermo Fisher Scientific) were cultured in
CHOgro® Expression Medium (Mirus Bio). Both cell lines were transiently transfected with plasmid DNA encoding
a human IgG1 internal control antibody, Bevacizumab or Fc-fusion construct using the TransIT-PRO® Transfection
Reagent (Mirus Bio) at a 1:1 reagent-to-DNA ratio (vol:wt) and 1 µg plasmid DNA per milliliter of culture in a
non-treated 6-well plate. All cultures were shifted to 32°C, shaking, immediately post-addition of the transfection
complexes and the CHOgro® Titer Enhancer to the culture. Day 14 supernatants were analyzed using a standard
sandwich human IgG ELISA. Error bars represent the standard deviation of triplicate technical replicates.
®

CHEMICAL TRANSFECTION

500
400

500
450
400
350
300
250
200
150
100
50
0

400

100

600

0

Alemtuzumab120

CHOgro® High Yield Expression System continued
12.0E + 06

100
90

10.0E + 06

80

9.0E + 06

70

8.0E + 06

60
50

7.0E + 06% Viable

Cells/ml

11.0E + 06

40

6.0E + 06

30
5.0E + 06

20

4.0E + 06

10

3.0E + 06

0
D1

D0

D2

D3

D4

D5

D6

D7

Days Post-transfection®
FIGURE 30. CHOgro
Titer Enhancer Does Not Adversely Affect Cell Growthand Viability Post-transfection. Triplicate
Viability, TransIT-PRO Alone
Counts, TransIT-PRO Alone
flasks of FreeStyle™
CHO-S cells adapted to CHOgro® Expression Medium
were transiently transfected with theViability, TransIT-PRO + CHOgro Titer Enhancer
Counts, TransIT-PRO + CHOgro Titer Enhancer
TransIT-PRO® Transfection Reagent (Mirus Bio) at a 1:1 reagent-to-DNA ratio (vol:wt) and 1 µg plasmid DNA per
milliliter of culture at 4 x 106 cells/ml in 125 ml Optimum Growth flasks (25 ml/flask, Thomson Instrument Company).
As indicated, CHOgro® Titer Enhancer was added and all of the cultures were shifted to 32°C immediately postaddition of the transfection complexes to the culture. Cell counts (solid line) and viability (propidium iodide staining,
dotted line) were measured daily post-transfection using a Guava® easyCyte™ 5HT flow cytometer (EMD Millipore).
®

®

®

®

®

®

1000
900
800

IgG (mg/L)

700
600
500
400
300
200
100
0
Vessel Size:

N/T 6-well

125 ml

1.6 L

5L

Culture Volume:

2 ml

20 ml

500 ml2000 ml

FIGURE 31. CHOgro® High Yield Expression System Enables 1,000-fold Scalability. Human IgG1 was produced bytransient transfection with the TransIT-PRO® Transfection Reagent (Mirus Bio) and 1 µg plasmid DNA per milliliterof culture at a 1:1 reagent:DNA ratio. Freestyle CHO cells (Thermo Fisher Scientific) were transfected at a density of
4 x 106 cells/ml in CHOgro® Expression Medium (Mirus Bio) at the following volumes/culture vessels: 2 ml/non-tissue
culture treated 6-well dish, 20 ml/125 ml Thomson flask, 500 ml/1.6 L Thomson flask, 2000 ml/5 L Thomson flask. All
cultures were shifted to 32°C, shaking, immediately post-addition of the transfection complexes and the CHOgro®
Titer Enhancer to the culture. Cultures were maintained at the appropriate shake speed for the remainder of theexperiment. Day 14 supernatants were analyzed using a standard sandwich human IgG ELISA. Error bars represent
the standard deviation of triplicate technical replicates.

22 Chemical Transfection | mirusbio.com

Large Scale Protein Production

TransIT-PRO® TRANSFECTION KITPRODUCT NO.

QUANTITYMIR 5700

1 ml• Easy to Use—Compatible with multiple media
formulations

MIR 5760

10 ml

To inquire about bulk pricing, please call+1.608.441.2852"• Total Cost Savings—Higher protein yieldtranslates to lower material and labor costs

We recently engineered a bispecific immunofusion for the treatment andelimination of leukemia stem cells. For this work we chose Trans IT-PRO ®
for antibody production of CHO-S cells based on the high protein yield weobtained. (Kuo et al, Protein Eng Des Sel. Oct 2012).

"

Jen-Sing Liu, Ph.D.,
Molecular Templates Inc.

Description
Decrease time to produce usable protein by maximizing target protein yields through transienttransfection. The TransIT-PRO® Transfection Kit uses animal origin free components designed forhigh and reproducible protein yield in suspension CHO and 293 derived cells. Since it is compatible
with varied media formulations, the same media can be used for both transient and stable
expression. TransIT-PRO® outperforms linear PEI in protein yield, while providing a cost-effective
alternative to FreeStyle™ MAX.
400

A.

Day 3
Day 5
Day 7

Human Fc (µg/L)

300
200
100
0

B.

TransIT-PRO® +PRO Boost Reagent—

PRO

25 kDa linear PEI

PEI

FS

S1

FreeStyle™ MAX

S2

S3

hIgG1

FIGURE 32. Achieve High Antibody Titers Using The TransIT-PRO® Transfection Kit in Suspension CHO Cells. IgG1 wasproduced by transient transfection using the TransIT-PRO® and PRO Boost Reagent (1:1:1, Mirus Bio), 25 kDa linear
PEI (6:1) or FreeStyle™ MAX (1:1, Thermo Fisher Scientific) transfection reagents according to the manufacturers' or
published protocol (reagent:DNA ratio). Transfections were performed using 1 µg plasmid DNA per milliliter of culture
and 0.5 x 106 cells/ml at the time of transfection. FreeStyle™ CHO-S cells (Thermo Fisher Scientific) were cultured
in 20 ml of FreeStyle™ CHO Expression medium (Thermo Fisher Scientific) in 125 ml shake flasks. (A) Day 3, 5 and 7
supernatants were clarified and analyzed using a human IgG-Fc sandwich ELISA. Error bars represent the standard
deviation of triplicate technical replicates for TransIT-PRO® and FreeStyle™ MAX, and duplicate technical replicatesfor kDa linear PEI. (B) Day 7 supernatants were clarified and analyzed by western blot. An IgG standard was included
for quantification estimate (S1= 1.6 mg/L, S2= 3.2 mg/L, S3 = 6.3 mg/L).

CHEMICAL TRANSFECTION• High Performance—Achieve high protein
yield in suspension CHO and 293 cell types

TransIT-PRO® Transfection Kit continued

1750
BD SelectTM CD1000 Medium

1500

BD SelectTM CHO Medium

1250

PowerCHO®2 CD Medium

Human Fc (µg/L)

FreeStyleTM CHO Expression Medium

ProCHOTM5 Medium

1000
750
500
250
0

TransIT-PRO® +PRO Boost Reagent

25 kDa Linear PEI

FreeStyleTM Max

FIGURE 33. TransIT-PRO® Provides High Performance Across Varied Media Formulations. FreeStyle™ CHO-S cells wereadapted to five representative growth media as noted in the graph. Cells were transfected with an IgG encoding plasmid using the TransIT-PRO® and PRO Boost Reagent (1:1:1, Mirus Bio), 25 kDa linear PEI (6:1, Polysciences) or FreeStyle™ MAX (1:1, Thermo Fisher Scientific) transfection reagents according to published protocol (reagent:DNA ratio).
Transfections were performed in 24-well deep well shaker blocks using 1 µg plasmid DNA per milliliter of culture and0.5 x 106 cells/ml at the time of transfection. Human IgG1 was quantitated from day 5 clarified supernatants andanalyzed by a human anti-Fc sandwich ELISA. Error bars represent the standard deviation of triplicate wells.

12

Yield (mg/L)

10
8
6
4
2
0
1

2

3

4

5

6

7

8

9

10

ProteinTransIT-PRO® Transfection Reagent (Mirus Bio)
293Fectin™ (Life Technologies)

FIGURE 34. Achieve High Protein Yields Using the TransIT-PRO® Transfection Kit in Suspension 293 Cells. Ten different
secreted (non‐antibody) proteins were transiently expressed in FreeStyle™ 293‐F cells (Thermo Fisher Scientific) using the TransIT‐PRO® (1.5:1, Mirus Bio) or 293fectin™ (2:1, Thermo Fisher Scientific) transfection reagents according
to manufacturers' protocol. Cells were grown in FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific) andtransfected at a density of 4 x 106 cells/ml. The scale of the transfection for each protein varied between 1‐6 L of
culture.
Data courtesy of a TransIT‐PRO® pharmaceutical customer.

24 Chemical Transfection & Electroporation | mirusbio.com

Plasmid DNA, RNA, siRNA and miRNA

INGENIO® EZPORATOR® AND ELECTROPORATION
KITS & SOLUTIONS• High Efficiency Electroporation—Deliver
DNA or RNA to hard-to-transfect, stem andprimary cells

Ingenio® EZporator ® Electroporation
SystemPRODUCT NO.

QUANTITY• Compatible with Most Conventional
Electroporation Devices—Use your existing
system including Lonza-Amaxa®, Bio-Rad® orHarvard BTX®

MIR 51000

1 System

Ingenio® Electroporation Kits for Amaxa®
Nucleofector ® II/2b Nucleofector Devices(solution, 0.2 cm cuvettes, cell droppers)

Description
Ingenio® Electroporation Solution facilitatesefficient and reliable delivery of nucleic acids toeukaryotic cells refractory to chemical transfection.
Ingenio® is a broad spectrum solution that supports
high efficiency electroporation with minimal toxicity
and replaces standard electroporation solutions
including phosphate buffered saline and serumfree media. Ingenio® Kits (include solution,
cuvettes and cell droppers) are compatible
with multiple instruments and facilitate a wide
range of applications requiring nucleic aciddelivery to cells. All Ingenio® products can bepurchased separately.

PRODUCT NO.

QUANTITYMIR 50112

25 RXNMIR 50115

50 RXNMIR 50118

100 RXN

Ingenio® Electroporation Kits for All
Other Electroporators, such as Bio-Rad®
and Harvard BTX®
(solution, 0.4 cm cuvettes, cell droppers)

PRODUCT NO.

QUANTITYMIR 50113

25 RXNMIR 50116

50 RXNMIR 50119

100 RXN

Ingenio® Electroporation Solution
PRODUCT NO.

QUANTITYMIR 50111

25 RXNMIR 50114

50 RXNMIR 50117

100 RXN

Ingenio® Electroporation Accessories
Cuvettes

The Ingenio® EZporator ® Electroporation System is designed to
be used with the Ingenio® Electroporation Solution which is ideal
for mammalian and insect cell transfection. Electroporation is
the method of choice for many hard-to-transfect cell types,
and the Ingenio® EZporator ® Electroporation System is a costeffective, straight-forward, open system that is perfect for any
lab seeking performance without breaking the bank.

PRODUCT NO.

SIZEQUANTITYMIR 50121

0.2 cm50 PK

MIR 50123

0.4 cm50 PK

Cell Droppers
PRODUCT NO.

QUANTITYMIR 50125

50 PK

To inquire about bulk pricing, please call+1.608.441.2852

ELECTROPORATION• Save Money and Reduce Research Costs
Without Sacrificing Performance—Ingenio®
Electroporation Solution is available as astand-alone solution or as part of a complete
kit with cuvettes and cell droppers

Ingenio® Electroporation Kits and Solutions continued

RNP:

+

+

+

K562

+

+

Jurkat

+

uncut
NSP

NSP

cut

Cleavageeﬃciency (%)

63

60

60

30

25

21

FIGURE 35. Efficient RNP Delivery with Electroporation Ingenio® Solution. K562 and Jurkat cells were electroporated
with a Cas9 protein/gRNA, ribonucleoprotein (RNP) complex, comprised of 750 nM Cas9 protein (EnGen® Cas9 NLS,
NEB) and 1500 nM pre-complexed two-part gRNA (IDT) targeting PPIB using the Ingenio® Electroporation Solution(Mirus Bio) and a Gene Pulser ® Xcell™ Eukaryotic System (Bio-Rad Laboratories, Inc.). Exponential pulse conditionsof 130V, 950 µF for K562 and 150V, 950 µF for Jurkat cells were applied to triplicate 0.2 cm cuvettes, 100 µl volume,
10 x 106 cells/ml +/- RNP complex. A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours
post-transfection. Non-specific bands (NSP) were observed in the negative control of both cell lines. Cleavage
efficiency was calculated based on the ratio of cleaved band intensities to the sum of cleaved and uncleaved band
intensities minus the average signal of the non-specific band(s) in negative control lanes.

A.

B.

26 Electroporation | mirusbio.com

FIGURE 36. High Efficiency Plasmid DNA
Electroporation of Human Induced Pluripotent
Stem (iPS) Cells using Ingenio®. The Ingenio®
Electroporation Kit (Mirus Bio) was used to transfect
2 x 106 iPS cells on the Amaxa® Nucleofector ® II/2b
Device (Lonza Group Ltd). Cells were electroporated
with 8 µg ZsGreen expressing plasmid (Clontech)in 100 µl and plated in 6-well plates at 0.33 x 106
cells/well. Cells were visualized 24 hours posttransfection and imaged under 4X objective with an
Olympus IX71® Inverted Microscope (Olympus
Corporation). Image is (A) green fluorescence. Cells
were also assayed 24 hours post-transfection on an
Accuri® Cytometer (Becton Dickenson and Company).
The histogram (B) shows unelectroporated cells(black line) compared to cells electroporated withplasmid using the Ingenio® Electroporation Kit(green line, Mirus Bio).
Data courtesy of Cellular Dynamics International.

Ingenio® Electroporation Kits and Solutions continued

EGFP Positive Cells (%)

100
80
60
40
20

*Ke
ratin
t Co
rtica ocyte
l Ne
uron
*ME
BHK F
-2
CHO 1
-K1
COS
HEK -7
-29
3
HeL
a
Hep
a-SE
A
Hep P
G2
HL60
HU
Jurk VEC
at E
6-1
K56
2
Imm
MCF
orta
7
lize
d K NIH-3T
erat
inoc 3
ytes
PC 12
SKBR3
SKN- M
C
THP
-1
Vero

0

*Ra

*Primary cell types

EGFP Positive Cells (%)

100
80
60
40
20
0

HL-60

K562

Jurkat E6-1

SK-N-MC

Ingenio® Solution using Gene Pulser XcellTM System
Ingenio® Solution using Amaxa Nucleofector® 2b Device
Amaxa Nucleofector® Solution V on Amaxa Nucleofector® 2b Device

FIGURE 38. The Ingenio® Solution Provides Comparable Efficiency on the Amaxa® Nucleofector ® II/2b Device.
Cells were electroporated in parallel with an EGFP reporter vector. Two electroporators were used with different
electroporation kits: the Ingenio® Electroporation Kit (Mirus Bio) was used in the Gene Pulser Xcell™ Eukaryotic
System (Bio-Rad Laboratories, Inc,) and the Amaxa® Nucleofector ® II/2b Device (Lonza Group Ltd); the Amaxa®
Nucleofector ® Kit V (Lonza Group Ltd) was used in the Amaxa® Nucleofector ® II/2b Device (Lonza Group Ltd), allaccording to manufacturers' recommendations. EGFP expressing cells were identified 24 hours post-electroporation
by flow cytometry and presented as a percentage of the live cell population. Experiments were performed in triplicate
on three separate days and the data averaged.

ELECTROPORATIONFIGURE 37. The Ingenio® Electroporation Kits are Ideal for Electroporation in Many Cell Types Using the Amaxa®
Nucleofector ® II/2b Device. Cells were assayed at 24 hours post-electroporation by flow cytometry and reported aspercentage of live cell population. Visit www.mirusbio.com/applications/electroporation for ideal pulse conditions.

Ingenio® Electroporation Kits and Solutions continued

EGFP Positive Cells (%)

100
80
60
40
20

*Ker

*HUV
EC
atino
cyte
*ME
A549 F
-Flpl
n
BHK21
CHO
-K1
COS7
HEK293
HeLa
Hep
G2
HL-6
0
Jurka
t E61
K562
MCF
-7
Imor
taliz
ed K NIH-3T3
erat
inoc
ytes
PC - 1
RAW 2
264.
7
SH-S
Y5Y
SKBR
3
SK-N
-MC
THP1
U937
Vero

0

*Primary cell types

FIGURE 39. The Ingenio® Electroporation Kits are Ideal for Electroporation in Many Cell Types Using the Bio-Rad®
GenePulser Xcell™ System. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry
and presented as a percentage of the live cell population. Visit www.mirusbio.com/applications/electroporation for
ideal pulse conditions.
A. 100
HL-60
K562
Jurkat E6-1
SK-N-MCEGFP Positive Cells (%)

80

60

40

20

0

Propidium Iodide Negative Cells (%)

B.

Ingenio®
Electroporation Solution

PBS

Gene Pulser®
Electroporation Buffer

100
HL-60
K562
Jurkat E6-1
SK-N-MC

80

60

40

20

0

Ingenio®
Electroporation Solution

PBS

Gene Pulser®
Electroporation Buffer

28 Electroporation & Virus Production | mirusbio.com

FIGURE 40. Ingenio® Kits Outperforms Other
Electroporation Solutions in Efficiency andViability. Cells were electroporated in parallelwith an EGFP reporter vector using either
Ingenio® Electroporation Solution (Mirus Bio),
PBS or GenePulser ® Electroporation Buffer(Bio-Rad Laboratories, Inc.) on the GenePulser
Xcell™ Eukaryotic System (Bio-Rad
Laboratories, Inc.). (A) EGFP expressing cells
were identified 24 hours post-electroporation
by flow cytometry and presented as apercentage of the live cell population. (B)Cells were assayed for viablility by propidium
iodide staining and flow cytometry analysis.
Error bars represent the standard deviation of
triplicate wells.

Ideal for Large-scale Virus Production for Preclinical and Early Phase Clinical Trials

TransIT-VirusGEN® SELECT TRANSFECTION REAGENT• Performance—Efficient DNA delivery forlarge-scale production of high-titer viral
vectors

PRODUCT NO.		

QUANTITYMIR 6730		

30 ml• Quality—Tested for performance, identity,
sterility, endotoxin and mycoplasma

MIR 6735		

150 ml

To inquire about bulk pricing, please call+1.608.441.2852• Reliability—Exceptional lot-to-lot
consistency• Flexibility—Compatible with different virus
production platforms and repeat filtration

IDEAL FOR USE IN BACULOVIRUS PRODUCTION

Description
The TransIT-VirusGEN® SELECT Transfection Reagent is designed for large-scale virus
production in preclinical and early-phase clinical trials and is identical in formulation to thefully synthetic and animal origin free, research-grade TransIT-VirusGEN® Transfection Reagent.
TransIT-VirusGEN® SELECT is tested for performance, identity, sterility, endotoxin andmycoplasma to streamline ancillary material qualification in viral vector manufacturing.
A.

B.

16.0E+06

Functional Titer (TU/ml of original culture)

Suspension Cell Culture

Functional Titer (TU/ml)

14.0E+06
12.0E+06
10.0E+06
8.0E+06
6.0E+06
4.0E+06
2.0E+06
0
3:1

4:1

2:1

4.5E+06
4.0E+06
3.5E+06
3.0E+06
2.5E+06
2.0E+06
1.5E+06
1.0E+06
5.0E+05
0
2:1

2:1

2:1

Adherent Cell CultureD.
6.0E+07

Functional Titer (TU/ml)

5.0E+07
4.0E+073.0E+072.0E+07
1.0E+07
0.0E+00
3:1

4:1

2:1

TransIT-VirusGEN®
PEIpro® A3
25 kDa PEI Competitor
SELECT

Functional Titer (TU/ml of original culture)C.

8.0E+07

6.0E+07

4.0E+072.0E+07

0.0E+00
2:1

4:1

2:1

TransIT-VirusGEN®
PEIpro® A3
25 kDa PEI Competitor
SELECT

FIGURE 41. TransIT-VirusGEN® SELECT Outperforms Competitor Reagents. For complete experimental details,
please visit: www.mirusbio.com/products/transfection/virus-production-transit-virusgen-select

VIRUS PRODUCTION

TransIT-VirusGEN®
SELECT
PEIpro® A3
25 kDa PEI Competitor

TransIT-VirusGEN®
PEIpro® A3
25 kDa PEI Competitor
SELECTTransIT-VirusGEN® SELECT Transfection Reagent continued
B.

1.4E+07
1.2E+07
1.0E+078.0E+06
6.0E+06
4.0E+06
2.0E+06
0.0E+00
Lot 1

Lot 2

Lot 3

Lot 4

Functional Titer (TU/ml of original culture)

Functional Lentivirus Titer (TU/ml)

A.

9.0E+06

6.0E+06

3.0E+06

0.0E+00
Lot 1

TransIT-VirusGEN® SELECT

Lot 2

Lot 3

Lot 4

TransIT-VirusGEN® SELECTFIGURE 42. Reliable Lot-to-Lot Consistency. (A) Lentivirus was produced using suspension FreeStyle™ 293-F
cells grown in FreeStyle™ F17 Medium and transfected with 3rd generation vectors pLKO.1-puro-CMV-TurboGFP™
transfer vector (Sigma) and ViraSafe™ Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, Cell Bio Labs)
at a 3:0.5:0.5:2 DNA ratio, 1 ug/ml total plasmid, using the TransIT-VirusGEN® SELECT Transfection Reagent (3:1,
vol:wt). Virus containing supernatant was used to transduce 293T/17 cells and GFP expression was measured at 72
hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer.
(B) AAV2 was produced using suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium and transfectedusing pAAV-hrGFP, pAAV-RC and pAAV-Helper plasmids (1:1:1 DNA ratio, 1.5 μg/ml, Agilent Technologies) using
TransIT-VirusGEN® SELECT Transfection Reagent (2:1, vol:wt). Harvested virus was used to transduce HT1080 cells
and GFP expression was measured 48 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer. For
both lentivirus and AAV, functional titers were measured from virus dilutions with less than 20% GFP positive cells.*
B.

1.0E+078.0E+06

6.0E+06

4.0E+06

2.0E+06
0.0E+00
Control +1 Filtration +2 Filtration +3 Filtration

TransIT-VirusGEN® SELECT

Functional Titer (TU/ml of original culture)

Functional Lentivirus Titer (TU/ml)

A.

1.2E+079.0E+06

6.0E+06

3.0E+06

0.0E+00
Control +1 Filtration +2 Filtration +3 Filtration

TransIT-VirusGEN® SELECTFIGURE 43. Compatible with Multiple Filtrations. (A) Lentivirus was produced using suspension FreeStyle™ 293-F
cells grown in FreeStyle™ F17 Medium and transfected with 3rd generation vectors pLKO.1-puro-CMV-TurboGFP™
transfer vector (Sigma) and ViraSafe™ Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, Cell Bio Labs)
at a 3:0.5:0.5:2 DNA ratio, 1 ug/ml total plasmid, using the TransIT-VirusGEN® SELECT Transfection Reagent (3:1,
vol:wt) that was filtered through a 0.22 µm polyethersulfone (PES) filter unit (Millipore Sigma) for the indicatednumber of times. Virus-containing supernatant was used to transduce 293T/17 cells and GFP expression wasmeasured at 72 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer.
(B) AAV2 was produced using suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium and transfectedusing pAAV-hrGFP, pAAV-RC and pAAV-Helper plasmids (1:1:1 DNA ratio, 1.5 μg/ml, Agilent Technologies) using
TransIT-VirusGEN® SELECT Transfection Reagent (2:1, vol:wt). Harvested virus was used to transduce HT1080 cells
and GFP expression was measured 48 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer. For
both lentivirus and AAV, functional titers were measured from virus dilutions with less than 20% GFP positive cells.**The error bars represent the range of triplicate wells. Suspension cells were at a density of 2 x106 cells/ml and adherent cells were approximately 85% confluent at the time of transfection.

30 Virus Production | mirusbio.com

TransIT-VirusGEN® SELECT Transfection Reagent continued

Compare TransIT-VirusGEN® Reagents
Therapeutic
Development Pipeline

Research &Development

Preclinical & Early
Phase Clinical Trial

Late Phase Clinical
Trial & Commercial
Manufacturing

Trans IT-VirusGEN®

Trans IT-VirusGEN® SELECT

Trans IT-VirusGEN® GMP

Trans IT-VirusGEN®
Reagents

Composition
AAV and LV Enhancer
Kits Available

Identical TransIT-VirusGEN® Reagent Formulation(ready to use, chemically defined, animal origin free in 77.5% ethanol)
Coming Soon

Available

R&D

Preclinical

GMP

Quality Control

Functional AAV titer assay• Functional AAV titer assay• Formulation identity• Appearance• Sterility: per USP <71>
• Bacterial endotoxin:
per USP <85>
• Mycoplasma: per USP <63>

• Validated formulation
identity assay• Appearance• Sterility: per USP <71>
• Bacterial endotoxin:
per USP <85>
• Mycoplasma: per USP <63>

Configuration

0.3 ml; 0.75 ml; 1.5 ml;
5 and 10 pack, 1.5 ml vials• 30 ml bottle• 150 ml bottle

150 ml bottle

Manufacturing

Research grade
manufacturing withaseptic filtration

Research grade
manufacturing withaseptic filtration

GMP validated manufacturing
process with sterile filtration

Quality

Raw Materials

Available
Documentation

Available
Analytical Assays

Research grade• Certificate of Analysis• Certificate of Origin
Includes: TSE/BSE
Statement• Certificate of Analysis• Certificate of Origin—

GMP grade criticalraw materials• Certificate of Analysis• Certificate of Origin
Includes: TSE/BSE
Statement• DMF Available in 2022 with
Quality Agreement• Validated identity assay• Residual reagent assay

All TransIT-VirusGEN® reagents are designed to enhance delivery of packaging and transfer vector DNA to suspension andadherent HEK 293 cell types in order to increase production of recombinant lentivirus and adeno-associated virus AAV.
TransIT-VirusGEN® SELECT Transfection Reagent is identical in formulation to our research-grade TransIT-VirusGEN®
and includes release testing and quality documentation to streamline the process of ancillary material qualification,
ensuring seamless transitions from discovery through large-scale manufacturing, making it the superior choice forlarge-scale virus production.

VIRUS PRODUCTION

Available

TransIT-VirusGEN® SELECT Transfection Reagent continued

Animal Origin Free and Quality Control Documentation
Below is an example of the Certificate of Origin and BSE/TSE Statement forTransIT-VirusGEN® SELECT Transfection Reagent documenting that it is chemically synthesized
and is not manufactured with any animal-derived components.
Also pictured is an example of the Certificate of Analysis for TransIT-VirusGEN® SELECT
Transfection Reagent that includes a functional assay and testing for: sterility, endotoxin andmycoplasma.

CERTIFICATE OF ORIGIN
Product Name:TransIT-VirusGEN® SELECT Transfection Reagent

Product Number:

MIR 6730

Lot Number:Animal Derived Components:

None

Type of Manufacture:

Chemical Synthesis

Country of Manufacture:

USA______________________________________________________________________________________________________________________________________________

This information is to be used for the purpose of determining animal origin only and not to be confused with“country of origin” for import/export purposes.

BSE TSE Statement
Product Name:TransIT-VirusGEN® SELECT Transfection Reagent

Product Number:

MIR 6730

Lot Number:
Issue Date:

This product is manufactured in Madison, WI (USA) entirely from material of non-animal origin. The manufacture,
packaging, storage and transportation of these materials do not involve the use of material of animal origin. Therefore, theseproducts have minimal risk of contamination with Bovine Spongiform Encephalopathy (BSE) or Transmissible Bovine
Encephalopathy (TSE).

CERTIFICATE OF ANALYSIS

Mirus Bio LLC does not have plans to change the production of this material in any way that would increase the risk of BSE
or TSE contamination.
Product Name:TransIT-VirusGEN® SELECT Transfection Reagent

Product Number:
Certified By:

Signed on behalf of Mirus Bio LLC By:

Susan Elder, Quality Control Supervisor

MIR 6730

Lot Number:Retest Date:

Storage-10fromto this-30°C
This product is sold to the Buyer with a limited license for research use and further manufacturing; not for administration
into humans.Condition:
This product, or parts
product, may not be re-packaged or re-sold without written permission from Mirus Bio LLC.
Rev. Example

Issue Date:

Susan Elder, Quality Supervisor

Quality Control Testing and Results

Test Description
Functional Assay1
Sterility Testing2
Endotoxin Testing3
Mycoplasma Testing4

Mirus Bio LLC│5602 Research Park Blvd, Ste 210│Madison, Wisconsin│53719│608.441.2852│FAX 608.441.2849│www.mirusbio.com

Specification> 500,000 TU/mL
No growth observed≤ 1 EU/mL
None detected

Result
Pass
Pass
Pass
Pass

References
1TransIT-VirusGEN® Transfection Reagent is tested for adeno-associated virus (AAV) production in suspension 293 cells using a
GFP encoding transfer vector. Functional virus titer is determined by transducing HT1080 cells and measuring GFP expression byflow cytometry.
2 Performed per USP <71> guidelines.
3

Performed per USP <85> guidelines.

4

Performed per USP <63> guidelines.

Mirus Bio LLC│5602 Research Park Blvd, Ste 210│Madison,
Wisconsin│53719│608.441.2852│FAX 608.441.2849│www.mirusbio.com
Certified By:

Susan Elder, Quality Control Supervisor
This product is sold to the Buyer with a limited license for research use and further manufacturing; not for administration into humans. This product, or parts from thisproduct, may not be re-packaged or re-sold without written permission from Mirus Bio LLC. For bulk inquiries, contact Mirus Bio LLC, 545 Science Drive, Madison, WI
53711. Email: sales@mirusbio.com
TransIT--VirusGEN® is a registered trademark of Mirus Bio LLC.

Mirus Bio LLC│5602 Research Park Blvd, Ste 210│Madison, Wisconsin│53719│608.441.2852│FAX 608.441.2849│www.mirusbio.com

32 Virus Production | mirusbio.com

Ideal for High Titer Virus Production

IDEAL FOR USE IN BACULOVIRUS PRODUCTION

TransIT-VirusGEN® TRANSFECTION REAGENT• Reliable—Consistent high titer virus
production

QUANTITYMIR 6703

0.3 ml• Scalable—Efficient across different formats

MIR 6704

0.75 ml• Flexible—Address different virus and cell
culture systems

MIR 6700

1.5 mlMIR 6705

5 x 1.5 ml

MIR 6706

10 x 1.5 ml

MIR 6720

30 ml

Description
TransIT-VirusGEN® Transfection Reagent is designed
to enhance delivery of packaging and transfer vectors
to adherent and suspension HEK 293 cell types toincrease recombinant adeno-associated virus (AAV)
and lentivirus production.
A.

PRODUCT NO.

To inquire about bulk pricing, please call+1.608.441.2852

AAV Production in Suspension 293-F Cell Culture B. AAV Production in Adherent 293T/17 Cell Culture
3.5E + 07

Functional Titer (TU/ml of original culture)

Functional Titer (TU/ml of original culture)

7E + 06
6E + 06
5E + 06
4E + 06
3E + 06
2E + 06
1E + 06

5E + 06

2 ml
6-well plate shaking

100 ml
250 ml flask shaking

2 ml
6-well plate

Culture Volume:
Culture Vessel:

AAV Production in Suspension 293-F Cell Culture

10 ml
10 cm dishA.
7.E + 07

4.E + 06

3.E + 06

2.E + 06

1.E + 06

5.E + 07
4.E + 07
3.E + 07
2.E + 07
1.E + 07
0.E + 00

0.E + 00
TransIT®-VirusGEN™

B.

Lentivirus Production in Suspension 293-F Cell Culture

6.E + 07

Lipofectamine
Competitor®B4
2000

Lipofectamine
Competitor®B5
3000

TransIT®-VirusGEN™

25 kDa PEI

AAV Production in Adherent 293T/17 Cell CultureB.
5.E + 07

Lipofectamine
Competitor®B4
2000

Lipofectamine
Competitor®B5
3000

25 kDa PEI

Lentivirus Production in Adherent 293T/17 Cell Culture

Functional Titer (TU/ml of original culture)

Functional Titer (TU/ml of original culture)

4.E + 07

3.E + 07

2.E + 07

1.E + 07

4.E + 07

3.E + 07

2.E + 07

1.E + 07

0.E + 00

0.E + 00
TransIT®-VirusGEN™

Lipofectamine
2000
Competitor®B4

Lipofectamine
3000
Competitor®B5

25 kDa PEI

FIGURE
45.
TransIT-VirusGEN®
Outperforms
Competitor Reagents in Suspension and Adherent AAVCell Cultures. For experimental details, please visit
www.mirusbio.com/virusgen

TransIT®-VirusGEN™

Lipofectamine
2000
Competitor®B4

Lipofectamine
3000
Competitor®B5

25 kDa PEI

FIGURE 46. TransIT-VirusGEN® Outperforms Competitor
Reagents in Suspension and Adherent Lentivirus
Cell Cultures. For experimental details, please visit
www.mirusbio.com/virusgen

VIRUS PRODUCTION

Functional Titer (TU/ml of original culture)

5.E + 06

10E + 06

Functional Titer (TU/ml of original culture)A.

2E + 07
1.5E + 07

0E + 00

0E + 00
Culture Volume:
Culture Vessel:

3E + 07
2.5E + 07

FIGURE 44. TransIT-VirusGEN® Enables
Broad Scalability (AAV). Suspension
FreeStyle™ 293-F cells grown in
FreeStyle™ F17 Medium (A) or adherent
293T/17 cells (B) were transfectedwith pAAV-hrGFP, pAAV-RC and pAAVHelper (1:1:1 ratio, 1.5 total µg/ml,
Agilent Technologies) using the TransITVirusGEN® Transfection Reagent (2:1
reagent-to-DNA ratio (vol:wt), Mirus) at
the indicated volumes per culture vessel.
For experimental details, please visit
www.mirusbio.com/virusgenIdeal for Recombinant Lentivirus Production

TransIT®-LENTI TRANSFECTION REAGENT• High Performance—Provide up to eight-foldhigher functional titers• Simple Protocol—No media change required,single harvest• Animal Origin Free—Regulatory friendly

Description
The TransIT®-Lenti Transfection Reagent isdesigned to enhance delivery of packaging andtransfer vectors to adherent HEK 293T cell types
and increase recombinant lentivirus production. The
TransduceIT™ Transduction Reagent enhances
recombinant lentivirus infection of target cells.

QUANTITYMIR 6603		

0.3 mlMIR 6604		

0.75 ml

MIR 6600		

1.5 mlMIR 6605		

5 x 1.5 ml

MIR 6606		

10 x 1.5 ml

TransduceIT™ Transduction Reagent
PRODUCT NO.

QUANTITYMIR 6620		

1 mlTransIT® Lentivirus System(System includes: TransIT-Lenti® Transfection
Reagent, TransduceIT™ Transduction Reagent,Lentivirus Packaging Mix Powered by MISSION©)

1.4E + 08
1.2E + 08

Functional Titer (TU/ml)

PRODUCT NO.		

1.0E + 08

PRODUCT NO.

QUANTITY

8.0E + 07

MIR 6650		

5 RXN

MIR 6655		

34 RXN

6.0E + 07
4.0E + 07
2.0E + 07
0
TransIT®-Lenti Lipofectamine
Competitor B4®
2000

Lipofectamine
Competitor B5®
3000

25 kDa PEI

CaPO4 ppt

FIGURE 47. High Functional Titers with the TransIT®-Lenti
Transfection Reagent. Adherent 293T/17 cells were transfected
in a 6-well plate with pLKO.1-puro-CMV-TurboGFP™ transfer
vector (Sigma-Aldrich, Inc. LLC) and the Lentivirus Packaging
Mix Powered by MISSION® (1:1 ratio, 2 µg/well) with thefollowing reagents: TransIT®-Lenti (3:1, vol:wt; Mirus Bio),
Competitor B4 (3:1), Competitor B5 (3:1:1), 25 kDa PEI (6:1)
or CaPO4 precipitation (4 µg pDNA/well). The supernatant was
harvested, filtered (0.45 µm), and titered using 293T/17 cells.
Lentivirus transductions were performed in the presence of 8 µg/
ml TransduceIT™ (Mirus Bio) and GFP expression was measured
72 hours post-transduction using guava® easyCyte™ 5HT Flow
Cytometer (MilliporeSigma). Error bars represent triplicate
transfection complexes titered individually. Functional titers were
calculated using virus dilutions with less than 20% GFP positive
cells.

34 Virus Production | mirusbio.com

Lentivirus Packaging Mix Powered by
MISSION®
PRODUCT NO.

QUANTITYMIR 6630		

5 RXN

MIR 6640		

34 RXNTo inquire about bulk pricing, please call+1.608.441.2852

IDEAL FOR USE IN BACULOVIRUS PRODUCTION

TransIT®-Lenti Transfection Reagent continued
100
90

GFP Positive (%)

80
70
60
50
40
30
20
10
0
TransIT®-Lenti

FIGURE 48. High Efficiency Transfection with the Trans IT ® -Lenti Transfection Reagent. Adherent
293T/17 cells were transfected in a 6-well plate format using MISSION® pLKO.1-puro-CMV-TurboGFP™ transfer
vector and Lentivirus Packaging Mix Powered by MISSION® (Sigma-Aldrich, Inc. LLC) using the TransIT®-Lenti
Transfection Reagent (3:1, vol:wt; Mirus Bio). GFP efficiency was measured at 48 hours post-transfection. Error
bars represent five transfection complexes. Images were captured at 48 hours post-transfection. The observed
cell rounding and cell-cell fusion is due to high expression of the vesicular stomatitis virus G protein (VSV-G) for
pseudotyping the recombinant lentivirus.

70,000,000

50,000,000
40,000,000
30,000,000
20,000,000
10,000,000
012-well plate

6-well plate

100mm dishTransIT®-Lenti

FIGURE 49. Lentivirus Production is Scalable. Adherent 293T/17 cells were transfected in a 12-well, 6-well or
100 mm plate format using the MISSION® vectors (pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus
Packaging Mix Powered by MISSION® at a 1:1 ratio; Sigma-Aldrich, Inc. LLC) and the TransIT®-Lenti Transfection
Reagent (3:1, vol:wt; Mirus Bio). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17
cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ (Mirus Bio) and GFP
expression was measured 72 hours post-transduction. Error bars represent triplicate transfection complexes titeredindividually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells

VIRUS PRODUCTION

Functional Titer (TU/ml)

60,000,000

TransIT®-Lenti Transfection Reagent continued
B.

A.
35

GFP Positive (%)

30
25
20
15
10
5
0

TransIT®-Lenti
Generated Virus

Lipofectamine® 2000
Generated Virus

FIGURE 50. High Transduction Efficiency with Unconcentrated Lentivirus Using Trans IT ® -Lenti. (A)Lentivirus was produced with the TransIT®-Lenti Transfection Reagent (3:1, vol:wt; Mirus Bio) or Lipofectamine®
2000 (Thermo Fisher Scientific) using the MISSION® vectors (pLKO.1-puro-CMV-TurboGFP™ transfer vector and theLentivirus Packaging Mix Powered by MISSION®, Sigma-Aldrich, Inc. LLC). The supernatant was harvested, filtered(0.45 µm) and frozen. Lentivirus transductions were performed 5 days post-plating with iCell® Motor Neurons (Cellular
Dynamics International (CDI, a FUJIFILM Company)). For both TransIT®-Lenti (Mirus Bio) and Lipofectamine® 2000
(Thermo Fisher Scientific), 1 µm of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency
was measured 72 hours post-transduction using guava® easyCyte™ 5HT Flow Cytometer (MilliporeSigma). Error
bars represent the SEM of duplicate wells. (B) iCell® Motor Neurons (Cellular Dynamics International (CDI, a FUJIFILM
Company)) were plated in 35 mm dishes (Ibidi) and transduced with lentivirus produced using the TransIT®-Lenti
Transfection Reagent (Mirus Bio) and MISSION® vectors (Sigma-Aldrich, Inc. LLC). Images were captured at 72 hours
post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.

CaPO 4Competitor B4

TransIT ® -Lenti

Concentrated
HIV CMVeGFP (1:100)

Concentrated
HIV CMVeGFP (1:1000)

FIGURE 51. Comparing Functionality of CaPO4, Competitor B4 or TransIT®-Lenti Generated Lentivirus. HIV CMVeGFP
virus was produced in HEK 293FT cells using either CaPO4, Competitor B4 or TransIT®-Lenti Transfection Reagent(Mirus Bio) per manufacturers' protocol. Lentivirus was collected 48 hours post-transfection and concentrated byultracentrifugation. HEK 293FT cells were infected with a 1:100 or 1:1000 dilution of each concentrated lentivirus.
Images (above) were captured 48 hours post-transduction.
Data courtesy of Jeremy Coffin, University of Iowa Viral Vector Core.

36 Virus Production | mirusbio.com

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PRODUCT LISTCHEMICAL TRANSFECTION

Transfection Into Any Cell Type With Any Nucleic Acid
Product
Product No.
TransIT-X2®
MIR 6003
Dynamic Delivery System
MIR 6004
MIR 6000
MIR 6005
MIR 6006
Plasmid DNA Transfection
Product
TransIT®-2020
Transfection Reagent

TransIT®-LT1
Transfection Reagent

Product No.
MIR 5404
MIR 5400
MIR 5405
MIR 5406
MIR 2304
MIR 2300
MIR 2305
MIR 2306

Quantity
0.3 ml0.75 ml
1.5 ml5 x 1.5 ml
10 x 1.5 ml
Quantity
0.4 ml1 ml
5 x 1 ml
10 x 1 ml
0.4 ml1 ml
5 x 1 ml
10 x 1 ml

Cell Line Specific			
Product
Product No.
Quantity
TransIT®-293
MIR 2704
0.4 mlTransfection Reagent
MIR 2700
1 ml
MIR 2705
5 x 1 ml
MIR 2706
10 x 1 ml
TransIT®-BrCa
MIR 5504
0.4 mlTransfection Reagent
MIR 5500
1 ml
MIR 5505
5 x 1 ml
MIR 5506
10 x 1 ml®
TransIT -CHO
MIR 2174
0.4 mlTransfection Kit*
MIR 2170
1 ml
MIR 2175
5 x 1 ml
MIR 2176
10 x 1 ml
TransIT-HeLaMONSTER®
MIR 2904
0.4 mlTransfection Kit*
MIR 2900
1 ml
MIR 2905
5 x 1 ml
MIR 2906
10 x 1 ml
Large-Scale Protein Production in Suspension CHO Cells
Complete System
Product No.
Quantity
CHOgro® High Yield
Expression System
MIR 6270
1 System
CHOgro® Expression System
MIR 6260
1 System
CHOgro® Components
CHOgro® Expression Medium
CHOgro® Liquid Polybag Format
CHOgro® Dry Powder Format
CHOgro® Transfection &Titer Enhancer Kit

Product No.
MIR 6200
MIR 6202
MIR 6201

Quantity
1 Liter
10 Liters
Prepares 10L

MIR 6225

1 Kit

Product
TransIT®-Jurkat
Transfection Reagent

Product No.
MIR 2124
MIR 2120
MIR 2125
MIR 2126
MIR 2804
MIR 2800
MIR 2805
MIR 2806
MIR 2164
MIR 2160
MIR 2165
MIR 2166

Quantity
0.4 ml1 ml
5 x 1 ml
10 x 1 ml
0.4 ml1 ml
5 x 1 ml
10 x 1 ml
0.4 ml1 ml
5 x 1 ml
10 x 1 ml

Insect Cell Transfection & Baculovirus Production
Product
Product No.
TransIT®-Insect
MIR 6104
Transfection Reagent
MIR 6100
MIR 6105
MIR 6106

Quantity
0.4 ml1 ml
5 x 1 ml
10 x 1 ml

TransIT®-Keratinocyte
Transfection Reagent

TransIT®-Oligo
Transfection Reagent

siRNA/miRNA
Product
TransIT-TKO®
Transfection Reagent

Product No.
MIR 2154
MIR 2150
MIR 2155
MIR 2156
MIR 2114
MIR 2110
MIR 2115
MIR 2116

Quantity
0.4 ml
1.5 ml5 x 1.5 ml
10 x 1.5 ml
0.4 ml
1.5 ml5 x 1.5 ml
10 x 1.5 ml

Large RNA (Viral and mRNA)		
Product
Product No.
TransIT®-mRNA
MIR 2225
Transfection Kit*
MIR 2250
MIR 2255
MIR 2256

Quantity
0.4 ml1 ml
5 x 1 ml
10 x 1 ml

TransIT-siQUEST®
Transfection Reagent

CHOgro® Components
TransIT-PRO®
Transfection Reagent
CHOgro® Complex
Formation Solution
Poloxamer 188 Solution
L-Glutamine Solution
Human IgG1 Expression Control

Product No.
MIR 5740
MIR 5750

Quantity
1 ml
10 ml

MIR 6210
MIR 6230
MIR 6240
MIR 6250

100 ml100 ml100 ml100 µgLarge-Scale Protein Production in Suspension CHO & HEK293 Cells
Product
Product No.
Quantity
TransIT-PRO®
MIR 5700
1 ml
Transfection Kit*
MIR 5760
10 ml

ELECTROPORATION

Product
Product No.
Size
Ingenio® Electroporation Kits
MIR 50112
25 RXN®
®
for Amaxa Nucleofector
MIR 50115
50 RXNII/2b Nucleofector Devices
MIR 50118
100 RXN(solution, 0.2 cm cuvettes, cell droppers)			
®
Ingenio Electroporation Kits
MIR 50113
25 RXN
for All Other Electroporators
MIR 50116
50 RXN
including EZporator®,
MIR 50119
100 RXN
Bio-Rad® and Harvard BTX®
(solution, 0.4 cm cuvettes, cell droppers)		

VIRUS PRODUCTION

Research-Grade Reagents and Kits
TransIT-VirusGEN®
Transfection Reagent

TransIT-VirusGEN® AAV Transfection Kit
TransIT-VirusGEN® LV Transfection Kit

SELECT-Grade Reagents and Kits
TransIT-VirusGEN® SELECT
Transfection Reagent
TransIT-VirusGEN® SELECT AAVTransfection Kit
TransIT-VirusGEN® SELECT AAVTransfection Kit
TransIT-VirusGEN® SELECT LV
Transfection Kit
TransIT-VirusGEN® SELECT LV
Transfection Kit

Product No.
MIR 6703
MIR 6704
MIR 6700
MIR 6705
MIR 6706
MIR 6720
MIR 6750
MIR 6760

Quantity
0.3 ml0.75 ml
1.5 ml5 x 1.5 ml
10 x 1.5 ml
30 ml
1 Kit
1 Kit

Product No.
MIR 6730
MIR 6735
MIR 6770
MIR 6730
MIR 6771
MIR 6775
MIR 6735
MIR 6771
MIR 6780
MIR 6730
MIR 6781
MIR 6782
MIR 6785
MIR 6735
MIR 6781
MIR 6782

Quantity
30 ml
150 ml
1 Kit
30 ml
1L
1 Kit
150 ml
1L
1 Kit
30 ml
1L
1L
1 Kit
150 ml1L
1L

Product
Ingenio® Electroporation
Solution

Product No.
Size
MIR 50111
25 RXN (6.25 ml)
MIR 50114
50 RXN (12.5 ml)
MIR 50117
100 RXN (25 ml)
Ingenio® Electroporation
MIR 50121
0.2 cm cuvettes (50 PK)
Accessories
MIR 50123
0.4 cm cuvettes (50 PK)
MIR 50125
Cell Droppers (50 PK)
			Ingenio® EZporator®
Electroporation System
MIR 51000
Complete System

GMP-Grade Reagents and Kits
TransIT-VirusGEN® GMP
Transfection Reagent
TransIT-VirusGEN® GMP
AAV Transfection Kit
TransIT-VirusGEN® GMP LV
Transfection Kit

Accessory Products
TransIT®-Lenti
Transfection Reagent

TransduceIT™
Transduction Reagent
TransIT® Lentivirus System
Lentivirus Packaging Mix
Powered by MISSION® Genomics

Product No.

Quantity

MIR 6845-GMP

150 mlMIR 6815-GMP
MIR 6845-GMP
MIR 6816-GMP
MIR 6825-GMP
MIR 6845-GMP
MIR 6826-GMP
MIR 6827-GMP

1 Kit
150 ml1L
1 Kit
150 ml1L
1L

Product No.
MIR 6603
MIR 6604
MIR 6600
MIR 6605
MIR 6606

Quantity
0.3 ml0.75 ml
1.5 ml5 x 1.5 ml
10 x 1.5 ml

MIR 6620
MIR 6650
MIR 6655
MIR 6630
MIR 6640

1 ml
5 RXN
34 RXN
5 RXN
34 RXNTO ORDER | Toll Free 888.530.0801 | Direct 608.441.2852 | www.mirusbio.com*TransIT Transfection Kits supplied with a transfection and boost reagent. To inquire about bulk pricing, please call +1.608.441.2852

052022