Genotypes of Frequently Used Bacterial Strains
Genotypes of Frequently Used Bacterial Strains.

TECHNICAL REFERENCE

All genes in the bacterium are presumed to be in the wildtype state, except for those listed, which are mutant alleles carried by that bacterium. Genes listed on theF′ episome, however, represent wildtype alleles unless specified otherwise. Strains are λ– unless specified otherwise.
StrainBL21(DE3)*BL21(DE3)pLysS*BMH 71-18 mutS
C600 (1)C600hfl (1)DH1 (2)
DH10B

GenotypeF–, ompT, hsdS B (rB–, mB–), dcm, gal, λ(DE3)F–, ompT, hsdS B (rB–, mB–), dcm, gal, λ(DE3), pLysS (Cmr)thi, supE, ∆(lac-proAB), [mutS::Tn10(tetr)] [F′, traD36, proAB, lacI q Z∆M15]
thi-1, thr-1, leuB6, lacY1, tonA21, supE44
thi-1, thr-1, leuB6, lacY1, tonA21, supE44, hflA150::Tn10(tetr)
recA1, endA1, gyrA96, thi-1, hsdR17 (rK–, mK+), supE44, relA1
F–, mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15, ∆lacX74, deoR, recA1, endA1, araD139, ∆(ara, leu)7697, galU, galK, λ-,rpsL(strr), nupG
DH5α™	φ80dlacZ∆M15, recA1, endA1, gyrA96, thi-1, hsdR17 (rK–, mK+), supE44, relA1, deoR, ∆(lacZYA-argF) U169, phoA
DM1 (3)	F′, dam -13 ::Tn9 (Cmr) dcm, mcrB, hsdr-M+, gal1, gal2, ara-, lac-, thr-, leu-, ton R, tsx R, Su oES1301 mutS
lacZ53, thyA36, rha-5, metB1, deoC, IN(rrnD-rrnE), [mutS201::Tn5]*HB101 (4)thi-1, hsdS20 (rB–, mB–), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20(strr), xyl-5, mtl-1
JM101 (5)supE, thi, ∆(lac-proAB), F′ (traD36, proAB, lacI qZ∆M15)*JM109 (5)endA1, recA1, gyrA96, thi-1, hsdR17 (rK–, mK+), relA1, supE44, ∆(lac-proAB), [F′, traD36, proAB, lacI qZ∆M15]
JM109(DE3) (5)	endA1, recA1, gyrA96, thi-1, hsdR17 (rK–, mK+), relA1, supE44, ∆(lac-proAB), [F′, traD36, proAB, lacI qZ∆M15], λ(DE3)JM110 (5)	rpsL(strr), thr, leu, thi, hsdR17 (rK–, mK+), lacY, galK, galT, ara, tonA, tsx, dam, dcm, supE44, ∆(lac-proAB), [F′, traD36, proAB,lacI qZ∆M15]
KRX	[F′, traD36, ∆ompP, proAB +, lacl q, ∆(lacZ)M15] ∆ompT, endAI, recAI, gyrA96 (Nalr ), thi-I, hsdR17 (r k –, m k+), el4– (mcrA), relAI,supE44, ∆(lac-proAB), ∆(rhaBAD)::T7 RNA polymerase
KW251
supE44, galK2, galT22, metB1, hsdR2, mcrB 1, mcrA, [argA81::Tn10(tetr)], recD1014
LE392 (6)hsdR514, (rK–, mK+), supE44, supF58, lacY1 or ∆(lacIZY)6, galK2, galT22, metB1, trpR55
NM522 (7)supE, thi, ∆(lac-proAB), ∆hsd5 (rK–, mK–), [F′, proAB, lacI qZ∆M15]
NM538 (8)supF, hsdR (rK–, m K+), trpR, lacY
NM539 (8)supF, hsdR (rK–, m K+), lacY, (P2)*Single Step (KRX)	[F′, traD36, ∆ompP, proAB +, lacl q, ∆(lacZ)M15] ∆ompT, endAI, recAI, gyrA96 (Nalr ), thi-I, hsdR17 (r k –, m k+), el4– (mcrA), relAI,supE44, ∆(lac-proAB), ∆(rhaBAD)::T7 RNA polymerase
Stbl2™
F–, mcrA, ∆(mcrBC-hsdRMS-mrr ), recA1, endA1, gyrA96, thi-1, supE44, relA1, λ–, ∆(lac-proAB)
Stbl4™	mcrA, ∆(mcrBC-hsdRMS-mrr), recA1, endA1, gyrA96, thi-1, supE44, relA1, λ–, ∆(lac-proAB), gal, F′{proAB +, lacl q, Z∆M15,Tn10(tet R)}®
SURE 	e14–, (mcrA–) ∆(mcrCB-hsdSMR-mrr)171, endA1, supE44, thi-1, gyrA96, relA1, lac, recB, recJ, sbcC, umuC::Tn5 (kan r), uvrC,[F′ proAB, lacl q Z∆M15::Tn10 (tet r)]
TOP10	F–, mcrA, ∆(mrr-hsdRMS-mcrBC), φ80lacZ∆M15, ∆lacX74, deoR, recA 1, araD139, ∆(ara , leu)7697, galU, galK, rpsL (strR), endA1,nupG
TOP10F′F′{laclq Tn10 (tetR)}, mcrA, ∆(mrr-hsdRMS-mcrBC), φ80lacZ∆M15, ∆lacX74, deoR, recA1, araD139, ∆(ara-leu)7697, galU, galK,rpsL (strr), endA1, nupG
XL1-BluerecA 1, endA1, gyrA96, thi-1, hsdR17(rK–, mK+), supE44, relA1, lac, [F′, proAB, lacl qZ∆M15::Tn10(tetr)]
XL10-Gold®
Tetr, ∆(mcrA)183, ∆(mcrCB-hsdSMR-mrr)173, endA1, supE44, thi-1, recA1, gyrA96, relA1, lac Hte [F′ proAB lacl qZ∆M15, Tn10
(Tet r) Amy Camr]
Y1089 (9)∆(lacU169), proA+, ∆(lon), araD139, strA, hflA150, [chr::Tn10(tetr)], (pMC9)Y1090 (9)∆(lacU169), proA+, ∆(lon), araD139, strA, supF, rpsL(strr), [trpC22::Tn10 (tetr)], (pMC9), hsdR (rK–, mK+)
*Strains available from Promega as competent cells are indicated by an asterisk. Strains shown in bold are available from Promega as glycerol freezer stocks.

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MiscellaneousF′ host contains an F′ episome with the stated features.
λ(DE3) bacteriophage λ carrying the gene for T7 RNA polymerase isintegrated into the host genome.
pMC9 is pBR322 with lacI q inserted and confers ampicillin and tetracycline
resistance.

References
1. Jendrisak, J., Young, R.A. and Engel, J. (1987) In: Guide to Molecular
Cloning Techniques, Berger, S. and Kimmel, A., eds., Academic Press,
San Diego, CA.
2. Hanahan, D. (1983) J. Mol. Biol. 166, 557.
3. Lorow-Murray, D. and Bloom, F. (1991) Focus 13, 20.
4. Lacks, S. and Greenberg, J.R. (1977) J. Mol. Biol. 114, 153.
5. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene 33, 103.
6. Murray, N., Brammar, W.J. and Murray, K. (1977) Mol. Gen. Genet. 150, 53.
7. Gough, J. and Murray, N. (1983) J. Mol. Biol. 166, 1.
8. Frischauf, A. et al. (1983) J. Mol. Biol. 170, 827.
9. Huynh, T., Young, R.A. and Davis, R. (1985) In: DNA Cloning, Vol. 1, Glover,D., ed., IRL Press Ltd., Oxford, UK.