HyClone classical media
FORMULATIONRPMI 1640 Medium
Liquid media
Product code
SH30027, SH30313
Component description

mg/Lmmol/L

Product code
SH30091
mg/Lmmol/L

Product code
SH30096mg/L

mmol/L

Inorganic salts
Calcium-(NO3)2-4H20

100

0.4235100

0.4235100

0.4235

Potassium chloride

400

5.3655400

5.3655400

5.3655Magnesium sulfate (anhydrous)

48.84

0.405648.84

0.405648.84

0.4056

Sodium chloride

6000

102.66946000

102.66946000

102.6694

Sodium phosphate dibasic (anhydrous)

800

5.6354800

5.6354800

5.6354

L-Arginine

200

1.1481200

1.1481200

1.1481

Amino acids
L-Asparagine (anhydrous)

50

0.3784500.3784

500.3784

L-Aspartic acid200.1503

200.1503

200.1503

L-Cystine-2HCl

65.15

0.208

65.15

0.208

65.15

0.208
0.1359L-Glutamic acid200.1359

200.1359

20

L-Glutamine

300

2.0527300

2.052700Glycine100.1332

100.1332

100.1332

L-Histidine FB

15

0.0967150.0967

150.0967

Hydroxy-L-proline

200.1525

200.1525

200.1525

L-Isoleucine

500.3812

500.3812

500.3812

L-Leucine

500.3812

500.3812

500.3812

L-Lysine-HCl

400.219400.219400.219

L-Methionine

150.1005

150.1005

150.1005

L-Phenylalanine

150.0908

150.0908

150.0908

L-Proline

200.1737200.1737200.1737

L-Serine

300.2855

300.2855

300.2855

L-Threonine

200.1679

200.1679

200.1679

L-Tryptophan

50.024550.024550.0245

L-Tyrosine-2Na-2H2 028.83

0.110428.83

0.110428.83

0.1104

L-Valine

200.1707

200.1707

200.1707

cytiva.com

RPMI 1640 Medium
Liquid media
Product code
SH30027, SH30313
Component description

mg/L

mmol/LD-Biotin

0.2

Calcium D-pantothenate

0.25

Choline chloride
Folic acid
Myo-inositol
Niacinamide

Product code
SH30091

Product code
SH30096mg/L

mmol/L

mg/L

mmol/L

0.00080.2

0.00050.25

0.00080.2

0.00080.00050.25

0.000530.0215

10.002330.021530.0215

10.0023

10.0023350.1943

10.0082

350.1943

350.1943

10.0082

10.0082

Pyridoxine-HCl

10.0049

10.0049

10.0049

Riboflavin
Thiamine-HCl

0.2

0.00050.2

0.00050.20.0005

10.00310.003

1

Vitamin B12

0.003

0.005

0.000004

0.005

0.000004

0.005

0.000004

D-Glucose (anhydrous)

2000

11.10122000

11.10122000

11.1012

Vitamins

Other
para-Aminobenzoic acid10.0073

10.0073

10.0073

L-Glutathione (reduced)

10.0033

10.0033

10.0033

Phenol red-Na

5.3

0.01415.30.0141

5.30.0141

HEPES

000

000

2000

23.80672000

23.80672000

23.8067

Sodium bicarbonate

2RPMI 1640 Medium
Liquid media
Product code
SH30203
Component description

mg/L

Product code
SH30255

mmol/Lmg/L

mmol/L

Product code
SH30605
mg/L

mmol/L

Inorganic salts
Calcium-(NO3)2-4H20

100

0.42351000.4235

1000.4235

Potassium chloride

400

5.3655400

5.3655400

5.3655Magnesium sulfate (anhydrous)

48.84

0.405648.84

0.405648.84

0.4056

Sodium chloride

6000

102.66946000

102.66946000

102.6694

Sodium phosphate dibasic (anhydrous)

800

5.6354800

5.6354800

5.6354

Amino acids
L-Arginine

200

1.1481200

1.1481200

1.1481

L-Asparagine (anhydrous)

50

0.378450

0.378450

0.3784

L-Aspartic acid200.1503

200.1503200.1503

L-Cystine-2HCl

65.15

0.208

65.15

0.208

65.15

0.208

L-Glutamic acid200.1359

200.1359

200.1359

L-Glutamine

00300

2.052700Glycine100.1332

100.1332

100.1332

L-Histidine FB

150.0967

150.0967

150.0967

Hydroxy-L-proline

200.1525

200.1525

200.1525

L-Isoleucine

500.3812

500.3812

500.3812

L-Leucine

500.3812

500.3812

500.3812

L-Lysine-HCl

400.219400.219400.219

L-Methionine

150.1005

150.1005

150.1005

L-Phenylalanine

150.0908

150.0908150.0908

L-Proline

200.1737200.1737200.1737

L-Serine

300.2855

300.2855

300.2855

L-Threonine

200.1679

200.1679

200.1679
0.0245

L-Tryptophan

50.024550.0245

5L-Tyrosine-2Na-2H2 028.83

0.110428.83

0.110428.83

0.1104

L-Valine

200.1707

200.1707

200.1707

D-Biotin

0.2

0.00080.2

0.00080.2

0.0008

Calcium D-pantothenate

0.25

0.00050.25

0.00050.25

0.0005Choline chloride

30.021530.021530.0215

Folic acid10.0023

10.002310.0023

Myo-inositol

350.1943

350.1943

35

0.1943

Niacinamide

10.0082

10.0082

10.0082

Vitamins

Pyridoxine-HCl

10.0049

10.0049

10.0049

Riboflavin0.2

0.00050.2

0.00050.2

0.0005

Thiamine-HCl

10.00310.00310.003

Vitamin B120.005

0.000004

0.005

0.000004

0.005

0.000004

D-Glucose (anhydrous)

2000

11.10122000

11.10122000

11.1012para-Aminobenzoic acid10.0073

10.0073

10.0073

L-Glutathione (reduced)

10.0033

10.0033

10.0033
0OtherPhenol red-Na

5.3

0.0141

5.3

0.01410HEPES

5958

25.00215958

25.002100

Sodium bicarbonate

2000

23.80672000

23.80672000

23.80673

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