RIKEN DNA BankRIKEN BioResource Center

RDB2136 S-HA-pRc/CMV
T7 promoter
HindIIITTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCCATGGCCTACCCCTACGACGTGCCCGACAlaSerMetAlaTyrProTyrAspValProAsp
single HA tag
NotIXbaI ApaI <- SP6 promoter
TACGCCGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
TyrAlaAlaAlaAlaArgAlaCysIle***RDB2137 S-T7-pRc/CMV
T7 promoter
HindIIITTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCCATGGCCAGCATGACCGGCGGCCAGCAGAlaSerMetAlaSerMetThrGlyGlyGlnGln
single T7 tag
NotI
XbaI ApaI <- SP6 promoter
ATGGGCGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
MetGlyAlaAlaAlaArgAlaCysIle***RDB2138 D-T7-pRc/CMV
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC
HindIII
AAGCTTCCATGGCCAGCATGACCGGCGGCCAGCAGATGGGCGCGGCCATGGCCAGCATGACCGGCGGCCAGCAGATGGGC
AlaSerMetAlaSerMetThrGlyGlyGlnGlnMetGlyAlaAlaMetAlaSerMetThrGlyGlyGlnGlnMetGly
double T7 tag
NotI
XbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***

RDB2139 D-HA-pRc/CMV
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC
HindIII
AAGCTTCCATGGCCTACCCCTACGACGTGCCCGACTACGCCGCGGCCTACCCCTACGACGTGCCCGACTAC
AlaSerMetAlaTyrProTyrAspValProAspTyrAlaAlaAlaTyrProTyrAspValProAspTyr
double HA tag
NotI
XbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAAlaAlaAlaLerAsnGlyProIleLeu***www.brc.riken.jp/lab/dna/RIKEN DNA BankRIKEN BioResource Center

RDB2140 S-Myc-pRc/CMV
T7 promoter
HindIIITTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCCATGGAGCAGAAGCTGATCAGCGAGGAG
AlaSerMetGluGlnLysLeuIleSerGluGlu
single Myc tag
NotI
XbaI ApaI <- SP6 promoter
GACCTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AspLeuAlaAlaAlaArgAlaCysIle***RDB2141 D-Myc-pRc/CMV
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCCHindIII
AAGCTTCCATGGAGCAGAAGCTGATCAGCGAGGAGGACCTGGCGGCCGAGCAGAAGCTGATAAGCGAGGAGGACCTG
AlaSerMetGluGlnLysLeuIleSerGluGluAspLeuAlaAlaGluGlnLysLeuIleSerGluGluAspLeu
double Myc tag
NotI
XbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***www.brc.riken.jp/lab/dna/RIKEN DNA BankRIKEN BioResource Center

RDB 5954 pCMV_S-Myc
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC

EcoRI* NcoI*NcoI*AAGCTGAATTCACCATGGCAGCTTCCATGGAGCAGAAGCTGATCAGCGAGGAGGACCTG
AlaGluPheThrMETAlaAlaSerMetGluGlnLysLeuIleSerGluGluAspLeu
single Myc tag
NotIXbaI ApaI <- SP6 promoter
GCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaArgAlaCysIle***RDB 5955 pCMV_D-Myc
T7 promoter
EcoRI* NcoI*TTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGAATTCACCATGGCA
AlaGluPheThrMETAla
NcoI*GCTTCCATGGAGCAGAAGCTGATCAGCGAGGAGGACCTGGCGGCCGAGCAGAAGCTGATAAGCGAGGAGGACCTGAlaSerMetGluGlnLysLeuIleSerGluGluAspLeuAlaAlaGluGlnLysLeuIleSerGluGluAspLeu
double Myc tag
NotIXbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***

RDB 5956 pCMV_S-FLAG
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC

EcoRI* NcoI*
AAGCTGAATTCACCATGGACTACAAGGACGATGACGATAAG
AlaGluPheThrMETAspTyrLysAspAspAspAspLys
single FLAG tag
NotI
XbaI ApaI <- SP6 promoter
GCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAlaAlaAlaArgAlaCysIle****They are not unique.

www.brc.riken.jp/lab/dna/RIKEN DNA BankRIKEN BioResource Center

RDB 6083 pRSV_S-FLAG
3'end of RSV promoter|EcoRI* NcoI*ACATTGGTGTGCACCTCCAAGCTGAATTCACCATGGACTACAAGGACGATGACGATAAG
AlaGluPheThrMETAspTyrLysAspAspAspAspLys
single FLAG tag
NotI
XbaI|5' end of BGH poly(A) signal
GCGGCCGCTCGAGCATGCATCTAGAGCTCGCTGATCAGCCTCGACTGTGCCAlaAlaAlaArgAlaCysIle**** They are not unique.

www.brc.riken.jp/lab/dna/