Frontiers in Genome
Engineering 201925-27 November 2019
Kobe, Japan

fge2019.jp

WELCOME MESSAGE
We are pleased to announce the 3rd "Frontiers in Genome Engineering" International Conference
to be held at the Kobe Convention Center, Hyogo, Japan from November 25 to 27, 2019. The
topics of the conference will include but not limited to the use and development of new genome
editing technologies for human health as well as agriculture and bioindustry. Following thesuccessful meetings in 2017 and 2018, we are expecting presentations by the world's leading
scientists and intense discussions among highly active participants of over 300 people at homeand abroad.

KEYNOTE SPEAKERS

MEET THE EDITORSINVITED SPEAKERS

CAUTION!
Due to Hanshin Expressway closure by construction on 20th Nov. to 29th Nov., the operation ofLimousine Buses from KIX is scheduled for delay or suspension. Please come with plenty oftime or use other transportation systems (Bay Shuttle Ferry or train).

PROGRAM

Program
MONDAY, NOVEMBER 25, 201914:00-20:00

Arrival and Registration

17:00-18:30

Welcome reception

18:30-18:40

Welcome and opening remarks

Keynote lecture

18:40-19:30

19:30-21:30

Chair : Osamu Nureki, The University of Tokyo, Japan
Feng Zhang, MIT, USA
Harnessing Nature’s Diversity For Gene Editing and Beyond
DrinksTUESDAY, NOVEMBER 26, 2019
Session 1: Technology and Tools-1

9:00-9:15

9:15-9:45

9:45-10:15

Chair : Jin-Soo Kim, Seoul National University, Korea

Keiji Nishida, Kobe University, Japan
Base editing, gene conversion and local diversification without DNA double strand break
Tomoji Mashimo, Osaka University, Japan
Type I CRISPR-Cas3 mediated genome editing in human cells
Keiichiro Suzuki, Osaka University, Japan
Development of in vivo genome editing technologies and application for genome-editing therapy
Kazuki Nakamae, Hiroshima University, Japan

10:15-10:30

Analysis of genomic and epigenomic features affecting the efficacy of MMEJ-assisted knock-in usingmachiato

10:30-10:45

Coffee break and poster session

Session 2: Technology and Tools-2

10:45-11:15

11:15-11:45

Chair : Keiji Nishida, Kobe University, Japan

Baohui Chen, Zhejiang University, China
Live Imaging of the Cellular Genome Using CRISPR Cas9 S ystems
Jin-Soo Kim, Seoul National University, Korea
Genome-wide target specificity of CRISPR RNA-guided base editors

11:45-12:15

Dave Savage, University of California Berkeley, USAHigh-throughput approaches for engineering CRISPR-Cas proteins
Osamu Nureki, The University of Tokyo, Japan

12:15-12:30

Molecular mechanism of CRISPR and structure-based development of genome editing tool towards
medical applications

12:30-12:45

12:45-13:00

Pranam Chatterjee, MIT Media Lab, USA
Robust genome editing with broad targeting CAS9 variants
Nozomu Yachie, The University of Tokyo, Japan
Recording cellular events in DNA

Poster Session
13:00-14:00

Lunch and poster session

14:00-15:30

Poster Session and Coffee breakSession 3: Technology and Tools-3

15:30-16:00

16:00-16:30

16:30-17:0017:00-17:15

17:15-17:30

Chair : Caixia Gao, CAS, China

Alexis Komor, University of California, USA
Investigating the Chemical and Cellular Mechanisms of Base Editing
Benjamin Kleinstiver, Harvard Medical School, USA
Expanding Genome Editing with Engineered CRISPR Enzymes
Knut Woltjen, CiRA, Japan
Templated and template-free precision editing of human gene variants
Martin Pacesa, University of Zurich, Switzerland
Structural basis SPCAS9 off-target binding
Yuichiro Miyaoka, Tokyo Metropolitan Institute of Medical Science, Japan
Precise deletion mutagenesis by dual CAS12A DNA cleavage

17:30-18:15

Meet-the-editor and poster session

18:30-21:30

BanquetKobe porpia hotel“Kairaku”

WEDNESDAY, NOVEMBER 27, 2019
Keynote lecture

9:00-9:45

Chair : Akihiko Kondo, Kobe University, Japan
Virginijus Siksnys, Vilnius University, Lithuania
Harnessing bacterial immune system for targeted genome engineering

Session 4: Regenerative Biology

9:45-10:15

10:15-10:45

10:45-11:15

11:15-11:30

Hideyuki Okano, Keio University, Japan
Non-human primate brain science using genome editing
Kathy Niakan, The Francis Crick Institute, UK
Using genome editing and single cell approaches to study early lineage specification in human embryos
Prashant Mali, Jacobs School of Engineering, USA
Improving genome interpretation via genome engineering toolsets: new approaches and new challenges
Coffee break and poster session

Session 5: Genome wide screening

11:30-12:00

12:00-12:30

12:30-13:00

13:00-13:45

14:15-14:45

14:45-15:00

15:00-15:15

Chair : Steve Mao, Science

Kosuke Yusa, Kyoto University, Japan
Development and application of CRISPR-knockout screening
Hyongbum Kim, Yonsei University College of Medicine, Korea
Deep learning-based prediction of CRISPR-Cpf1 and Cas9 activities
Wensheng Wei, Peking University, China
Gene Editing: High-throughput Functional Genomics and Beyond
Lunch and poster session

Session 6: Animals and plants

13:45-14:15

Chair : Tomoji Mashimo, Osaka University, Japan

Chair : Markus Elsner, Nature Biotechnology

Hui Yang, Institute of Neuroscience, China
DNA base editing induces substantial DNA&RNA off-target mutations and eliminated by mutagenesis
Caixia Gao, CAS, China
Base editing and Crop Improvement
Hiroaki Saika, National Agriculture and Food Research Organization, Japan
Targeted deletion of rice retrotransposon
Lee Hickey, The University of Queensland

Express editing for crop improvement
Kenta Sumiyama, RIKEN, Japan
15:15-15:30

Triple-target CRISPR enabled production of complete bi-allelic double and triple gene knockouts at firstgeneration

15:30-15:45

Coffee break and poster session

Session 7: Therapeutics

15:45-16:15

16:15-16:45

16:45-17:15

Chair : Nozomu Yachie, The University of Tokyo, Japan

Izuho Hatada, Gunma University, Japan
Epigenome editing of animals
Jacob Corn, ETH Zurich, Switzerland
Genome editing at work in human cells
Debojyoti Charkraborty, CSIR-Institute of Genomics and Integrative Biology, India
Highly precise genome editing using an engineered orthogonal Cas9 protein
Yutaka Hanazono, Jichi Medical University, Japan

17:15-17:30

Feasibility Study of Homologous Recombination-Based Genome-Editing Therapy in a Swine Model ofX-SCID

17:30-18:15

Meet-the-editor and poster session
Closing remarks

Poster presentations
P01
Seiichi Hirano (The University of Tokyo)
STRUCTURAL BASIS FOR THE PROMISCUOUS PAM RECOGNITION BY C. DIPHTHERIAE CAS9P02
Hiroshi Nishimasu (The University of Tokyo)
STRUCTURE-BASED ENGINEERING OF CRISPR-CAS9P03
Nahye Kim (Yonsei University College of Medicine)
SPCAS9 ACTIVITY PREDICTION BY DEEPSPCAS9, A DEEP LEARNING-BASED MODEL WITH HIGH
GENERALIZATION PERFORMANCEP04
Ryoya Nakagawa (The University of Tokyo)
BIOCHEMICAL CHARACTERIZATION AND MOLECULAR ENGINEERING OF THE MINIMAL CAS9
FROM C.JEJUNIP05
Hui Kwon Kim (Yonsei University College of Medicine)
HIGH-THROUGHPUT COMPARATIVE ANALYSIS OF xCas9, SpCas9-NG, AND SpCas9 ACTIVITIES IN
HUMAN CELLS
P06
Hideto Mori (The University of Tokyo)
SPADE FOR EXPLORING PERIODIC SEQUENCE REPEATS AS POTENTIAL GENOME EDITINGMODULESP07
Keishi Osakabe (Tokushima University)
GENOME EDITING IN PLANTS BY USING A NOVEL GENOME EDITING TOOL, TIDP08
Nina Kurihara (The University of Tokyo)
BIOCHEMICAL ANALYSIS OF CAS12C
P09
Kazuto Yoshimi (Osaka University)
CHARACTERIZATION OF CRISPR-CAS3-MEDIATED GENOME EDITING IN MAMMALIAN CELLSP10
Naoyuki Miyashita (KINDAI University)
MOLECULAR DYNAMICS SIMULATION STUDY FOR THE DIFFERENCES BETWEEN THE DYNAMICS
OF CRISPR CAS3 WITH AND WITHOUT SHORT SINGLE-STRAND DNA

P11Satoru Takeda (The University of Tokyo)
THE FUNCTIONAL AND STRUCTURAL ANALYSIS OF MINIATURE CRISPR-Cas14aP12
Lapatrada Taemaitree (Suez University)
Split-and-Click sgRNAsP13
Atsushi Kunii (Hiroshima University)
POWERFULGENE

ACTIVATIONVIACRISPR-BASEDHIERARCHICAL

ACCUMULATIONOF

EFFECTOR DOMAINS
P14
Ha rim Shin (University of Ulsan College of Medicine)
INHIBITION OF HISTONE DEACETYLATION IMPROVE CRISPR-MEDIATED ADENINE BASE EDITINGEFFICIENCY
P15
Jonathan Strecker (Broad Institute of MIT and Harvard)
RNA-guided DNA insertion with CRISPR-associated transposases
P16
Takahiro Otabe (The University of Tokyo)
SPLIT-CPF1 SYSTEM AS AN INDUCIBLE GENOME EDITING AND EFFICIENT GENE ACTIVATION
P17Ji-Eun See (University of Ulsan College of Medicine)
Reporter-based drug screening to improve CRISPR-mediated adenine base editing efficiency
P18
Niels R. Weisbach (ETH Zurich)
MULTIPLEXED GENOME ENGINEERING BY CAS12A AND CRISPR ARRAYS ENCODED ON SINGLE
TRANSCRIPTSP19
Hiroyuki Konishi (Aichi Medical University School of Medicine)
TANDEM PAIRED NICKING USING CAS9 NICKASES PERMITS TARGETED KNOCK-IN WITHOUTSIGNIFICANT INHIBITION BY P53
P20
Yanpeng Wang (Chinese Academy of Sciences)CYTOSINE, BUT NOT ADENINE, BASE EDITORS INDUCE GENOME-WIDE OFF-TARGET MUTATIONSIN RICEP21
You Kyeong Joeong (Hanyang University)
Adenine base editors catalyze cytosine conversions in human cells
P22
Changyang Zhou (Chinese Academy of Sciences)
OFF-TARGET RNA MUTATION INDUCED BY DNA BASE EDITING AND ITS ELIMINATION BY
MUTAGENESIS
P23
Ju-Chan Park (Seoul National University)
Safe Scarless Cassette-free Selection of Genome-edited Human Pluripotent Stem Cells Using Temporary
Drug ResistanceP24
John Yu (Chang Gung Memorial Hospital)
B3GALT5 GENOMIC KNOCKOUT ALTERS GLYCOSPHINGOLIPID PROFILE AND FACILITATESTRANSITION TO HUMAN NAÏVE PLURIPOTENCY
P25
Marco Jost (University of California)
CRISPR-BASED GENETIC SCREENS IN PRIMARY HUMAN DENDRITIC CELLS TO DECIPHERMECHANISMS OF HOST-MICROBIOTA INTERACTIONSP26
Yan Zhou (Wellcome Sanger Institute)
MEASURING GERMLINE VARIATION OF HUMAN GENE ESSENTIALITY BY GENOME-WIDECRISPR/CAS9 SCREENING IN INDUCED PLURIPOTENT STEM CELLSP27
Gue-ho Hwang (Hanyang University)
WEB TOOLS FOR CRISPR: RGENOME.NETP28
Jun Teramoto (Kobe University)
A COMPUTATIONAL TOOL TO DESIGN UNIQUE GUIDE RNAS FOR GENOME-WIDE BASE EDITING BY
TARGET-AIDP29
Ko Ishihara (Epigeneron, Inc.)A NOVEL SCREENING METHOD FOR GENOME-EDITED CELLS BY OLIGORIBONUCLEOTIDEINTERFERENCE-PCR (ORNi-PCR)P30Xiaomin

Si (University of Chinese Academy of Sciences)

GENOME EDITING OF UPSTREAM OPEN READING FRAMES ENABLES TRANSLATIONAL CONTROLIN PLANTS
P31
Yuriko Osakabe (Tokushima University)
DEVELOPMENT OF IN PLANTA-REGENERATION SYSTEM FOR PLANT GENOME EDITINGP32
Rui Zhang (Chinese Academy of Sciences, Beijing)
GENERATION OF HERBICIDE TOLERANCE TRAITS IN RICE BY BASE-EDITING
P33
Ayako Okuzaki (Tamagawa University)
CRISPR/Cas9 mediated genome editing of the SP11 gene in Broccoli
P34
Tomoko Miyaji (Tokushima University)
GENOME EDITING OF THE MODEL STRAWBERRY ‘FRAGARIA VESCA’ USING PLANT-OPTIMIZEDCRISPR/CAS9 SYSTEM
P35
Takuya Tsubota (National Agriculture and Food Research Organization)
DEVELOPMENT OF GENOME EDITING TECHNOLOGIES IN THE SILKWORM
P36
Takaya Abe (RIKEN)
Harnessing the CRISPR/Cas9 System in Mouse Genome Engineering @ LARGE, RIKEN BDR
P37
Naoki Wada (Tokushima University)
Development of a novel genome editing tool, TiD system, for mammalian genome engineering
P38
Shinya Ayabe (RIKEN)
MUTANT MOUSE CREATION IN RIKEN BRC FOR THE INTERNATIONAL MOUSE PHENOTYPINGCONSORTIUM
P39Shuji Takada (National Research Institute for Child Health and Development)
SCREENING OF THE RESPONSIBLE SEQUENCES FOR DISORDERS OF SEX DEVELOPMENT BY
GENERATION OF A 70 KB DUPLICATION AND FINE MAPPING USING MOUSE AS A MODELP40
Ramu Gopalappa (Yonsei University College of Medicine)
ENBLOCANDSEGMENTAL

DELETIONS

OF

HUMAN

XISTREVEALX

CHROMOSOME

INACTIVATION-INVOLVING RNA ELEMENTS
P41
Gyeong-Min Gim (Seoul National University)
GERMLINE TRANSMISSION OF CAS9-TRANSGENIC CATTLE AND ITS APPLICATION
P42
Eugene Chung (Institute for Basic Science, Seoul, Republic of Korea)
GENERATION OF CANINE DYSTROPHINOPATHY MODEL BY NUCLEAR TRANSFER USINGCRISPR/CAS9-MEDIATED SOMATIC CELLSP43
Yoshiki Miyasaka (Osaka Univ.)
EFFICIENT AND SIMPLE KNOCK-IN METHODS EXPAND THE POTENTIAL OF ANIMAL MODELS.
P44
Choongil Lee (Seoul National University)
CRISPR-PASS: GENE RESCUE OF NONSENSE MUTATIONS USING ADENINE BASE EDITORS.
P45
Suvd Byambaa (Jichi Medical University)
PHENOTYPICAL CORRECTION OF X-SCID MICE AFTER CRISPR/CAS9-MEDIATED UNIVERSALGENOME-EDITING THERAPY OF HEMATOPOIETIC STEM CELLSP46
Sung-ah Hong (Hanyang University)
COL7A1 GENE EDITING IN PATIENT-DERIVED FIBROBLASTS FOR RECESSIVE DYSTROPHICEPIDERMOLYSIS BULLOSA (RDEB) RESCUE USING NG PAM TARGETABLE ADENINE BASE EDITOR
P47
Takafumi Hiramoto (Jichi Medical University)
THERAPEUTIC GENE CORRECTION OF HUMAN HEMOPHILIA B IPSCS BY BASE-EDITINGAPPROACH BASED ON AN ENGINEERED CAS9 WITH BROAD PAM FLEXIBILITY
P48
Christopher J. Vavricka (Kobe University)
GENOME-SCALE

PREDICTIONOFGENEONTOLOGYFROMYEASTKNOCKOUTFINGERPRINTS
P49
Hiromasa Hara (Jichi Medical University)
ESTABLISHMENT OF SIMULTANEOUS TRIPLE-REPORTER SYSTEM FOR HDR/NEHJ/DSB

MASS