TwinSpin pre-ﬁlled with Density MediaDGM

Quantitymax. Vol.

Order No.

Leuko Spin

50 Tubes

6 ml

45-91006-10

PBMC Spin

50 Tubes

6 ml

45-92006-10

PBMC 24+

50 Tubes

6 ml

45-93006-10

PLT Spin

50 Tubes

6 ml

45-94006-10

Facts & Features

Fill-Spin-DropwinSpin
The NEW kind of Density Gradient Separation

 Combines density gradient cell separation withthe precision of pipetting✓ Enrich cells with high yield and a maximum viability✓ Usable with standard protocols for density
gradient centrifugation✓ Isolate from whole blood, buﬀy coat or cord blood✓ Enrichment of untouched speciﬁc cells in combination
with pluriSpin negative cell separation✓ Usable for sample preparation for magneticcell separation✓ No training or special equipment required

Pre-ﬁlled with Density Gradient Mediafor Cell Enrichment
Spin Medium

Enriched Cell Population

Leuko Spin Medium

All Leukocytes (PBMC, PMNC,granulocytes)

PBMC Spin Medium

Mononuclear cells (PBMC)

PBMC 24+ Spin Medium

Mononuclear cells from
8 - 48 hours old peripheral blood

PLT Spin Medium

Platelets

PBMC (peripheral blood mononuclear cells), PMNC (polymorphonuclear cells)

pluriSelect Life ScienceWorldwide
Deutscher Platz 5c04103 Leipzig, Germany

Pluriselect-USA, Inc
North-America
2030 Gillespie Way, Suite 103
El Cajon, CA 92020, USA

Phone: +49 341 333858-0
support@pluriselect.com

Phone: +1 619 202 4297
support@pluriselect-usa.com

www.pluriselect.com/products/twinspin.html

pluriSelect develops products optimized
for cell and particle separation for life sciences
and biomedical research.

Workﬂow

1,600,000
1,000,000

SSC - A

500,000

PBMC Spin

0500,000

1,000,000

Whole Blood0SSC - A

1,600,000

Data02,000,000

4,000,000

6,000,000

2,000,000

0FSC - A

1,600,000
1,000,000

SSC - ASpin 15 min 800xg, room
temperature, brake on

PLT Spin

0500,000

Close ﬁrmly with elasticcap (provided separately)

1,000,000

Remove stopper Pipettesample on top DGM

6,000,000

Fig. 2: Enriched PBMC with PBMC Spin Medium*

Leuko Spin

500,000

302

SSC - A

1,600,000

Fig. 1: Whole blood, major cell populations*

14,000,000

FSC - A

02,000,000

4,000,000

6,000,000

2,000,000

0FSC - A

4,000,000

6,000,000

FSC - A

Fig. 3: Enriched white blood cells with Leuko
Spin Medium*Fig. 4: Enriched platelets with PLT Spin Medium*

The major white blood cell populations of whole blood (Fig. 1) with interest for
research and development are lymphocytes (black), monocytes (pink), granulocytes (blue) and platelets (green). The usage of the Spin Media allows enriching
the diﬀerent cell populations for a wide range of downstream applications.

Collect target cells inseparate tube

Desired cells

PlasmaRBCs andunwanted cells

1,600,000

Advantages using TwinSpin
 Best Cost-Value-Ratio
 Minimum handling - minimum cell stress No shaky pipetting Ideal for small cell counts
 Customized pre-ﬁlling possible

00Density gradientmedium (DGM)

500,000

Push down on cap until
DGM drops out

PBMC 24+ Spin

1,000,000

Unscrew and separate
dropper

PBMC Spin
SSC - A

6

1,000,000

5500,000

4SSC - A

1,600,000

PBMC 24+ Spin Medium - access to old blood - improve your results

0

2,000,000

4,000,000

6,000,000

02,000,000

4,000,000

6,000,000

FSC - AFSC - A

Fig. 5: Enriched PBMC with PBMC Spin
Medium from blood 24 hours aﬅer blood donation*

Fig. 6: Enriched PBMC with PBMC 24+ Spin
Medium from blood 24 hours aﬅer blood donation*

Most density gradient media are limited for the use of fresh whole blood.
PBMC 24+ Spin Medium is recommended for the use of whole blood that is older
than 8 hours. This medium helps to reduce the time depended contamination ofthe PBMC fraction with granulocytes and debris (see Fig. 5 and Fig. 6).
*Flow cytometry analysis was gated on CD45+ cells.