Program
MONDAY, NOVEMBER 25, 201914:00-20:00

Arrival and Registration

17:00-18:30

Welcome reception

18:30-18:40

Welcome and opening remarks

Keynote lecture

18:40-19:30

19:30-21:30

Chair : Osamu Nureki, The University of Tokyo, Japan
Feng Zhang, MIT, USA
Harnessing Nature’s Diversity For Gene Editing and Beyond
DrinksTUESDAY, NOVEMBER 26, 2019
Session 1: Technology and Tools-1

9:00-9:15

9:15-9:45

9:45-10:15

Chair : Jin-Soo Kim, Seoul National University, Korea

Keiji Nishida, Kobe University, Japan
Base editing, gene conversion and local diversification without DNA double strand breakTomoji Mashimo, Osaka University, Japan
Type I CRISPR-Cas3 mediated genome editing in human cells
Keiichiro Suzuki, Osaka University, Japan
Development of in vivo genome editing technologies and application for genome-editing therapy
Kazuki Nakamae, Hiroshima University, Japan

10:15-10:30

Analysis of genomic and epigenomic features affecting the efficacy of MMEJ-assisted knock-in using
machiato

10:30-10:45

Coffee break and poster session

Session 2: Technology and Tools-2

10:45-11:15

11:15-11:45

Chair : Keiji Nishida, Kobe University, Japan

Baohui Chen, Zhejiang University, China
Live Imaging of the Cellular Genome Using CRISPR Cas9 S ystems
Jin-Soo Kim, Seoul National University, Korea
Genome-wide target specificity of CRISPR RNA-guided base editors

11:45-12:15

Dave Savage, University of California Berkeley, USA
High-throughput approaches for engineering CRISPR-Cas proteins
Osamu Nureki, The University of Tokyo, Japan

12:15-12:30

Molecular mechanism of CRISPR and structure-based development of genome editing tool towards
medical applications

12:30-12:45

12:45-13:00

Pranam Chatterjee, MIT Media Lab, USA
Robust genome editing with broad targeting CAS9 variants
Nozomu Yachie, The University of Tokyo, Japan
Recording cellular events in DNA

Poster Session
13:00-14:00

Lunch and poster session

14:00-15:30

Poster Session and Coffee breakSession 3: Technology and Tools-3

15:30-16:00

16:00-16:30

16:30-17:00

17:00-17:15

17:15-17:30

Chair : Caixia Gao, CAS, China

Alexis Komor, University of California, USA
Investigating the Chemical and Cellular Mechanisms of Base Editing
Benjamin Kleinstiver, Harvard Medical School, USA
Expanding Genome Editing with Engineered CRISPR Enzymes
Knut Woltjen, CiRA, Japan
Templated and template-free precision editing of human gene variants
Martin Pacesa, University of Zurich, Switzerland
Structural basis SPCAS9 off-target binding
Yuichiro Miyaoka, Tokyo Metropolitan Institute of Medical Science, Japan
Precise deletion mutagenesis by dual CAS12A DNA cleavage

17:30-18:15

Meet-the-editor and poster session

18:30-21:30

BanquetKobe porpia hotel“Kairaku”

WEDNESDAY, NOVEMBER 27, 2019
Keynote lecture

9:00-9:45

Chair : Akihiko Kondo, Kobe University, Japan
Virginijus Siksnys, Vilnius University, Lithuania
Harnessing bacterial immune system for targeted genome engineering

Session 4: Regenerative Biology

9:45-10:15

10:15-10:4510:45-11:15

11:15-11:30

Hideyuki Okano, Keio University, Japan
Non-human primate brain science using genome editing
Kathy Niakan, The Francis Crick Institute, UK
Using genome editing and single cell approaches to study early lineage specification in human embryos
Prashant Mali, Jacobs School of Engineering, USA
Improving genome interpretation via genome engineering toolsets: new approaches and new challenges
Coffee break and poster session

Session 5: Genome wide screening

11:30-12:00

12:00-12:30

12:30-13:00

13:00-13:45

14:15-14:45

14:45-15:00

15:00-15:15

Chair : Steve Mao, ScienceKosuke Yusa, Kyoto University, Japan
Development and application of CRISPR-knockout screening
Hyongbum Kim, Yonsei University College of Medicine, Korea
Deep learning-based prediction of CRISPR-Cpf1 and Cas9 activities
Wensheng Wei, Peking University, China
Gene Editing: High-throughput Functional Genomics and Beyond
Lunch and poster session

Session 6: Animals and plants

13:45-14:15

Chair : Tomoji Mashimo, Osaka University, Japan

Chair : Markus Elsner, Nature Biotechnology

Hui Yang, Institute of Neuroscience, China
DNA base editing induces substantial DNA&RNA off-target mutations and eliminated by mutagenesisCaixia Gao, CAS, China
Base editing and Crop Improvement
Hiroaki Saika, National Agriculture and Food Research Organization, Japan
Targeted deletion of rice retrotransposon
Lee Hickey, The University of Queensland

Express editing for crop improvement
Kenta Sumiyama, RIKEN, Japan
15:15-15:30

Triple-target CRISPR enabled production of complete bi-allelic double and triple gene knockouts at firstgeneration

15:30-15:45

Coffee break and poster session

Session 7: Therapeutics

15:45-16:15

16:15-16:45

16:45-17:15

Chair : Nozomu Yachie, The University of Tokyo, Japan

Izuho Hatada, Gunma University, JapanEpigenome editing of animals
Jacob Corn, ETH Zurich, Switzerland
Genome editing at work in human cells
Debojyoti Charkraborty, CSIR-Institute of Genomics and Integrative Biology, India
Highly precise genome editing using an engineered orthogonal Cas9 protein
Yutaka Hanazono, Jichi Medical University, Japan

17:15-17:30

Feasibility Study of Homologous Recombination-Based Genome-Editing Therapy in a Swine Model ofX-SCID

17:30-18:15

Meet-the-editor and poster session
Closing remarks