Catalog

Port-a-Patch
Accessories and Add-ons

For quotations and orders email:orders@nanion.de1

QSG_04008_000_Pap_Catalog_Accessories_And_Add-Ons.docx

External Perfusion/Temperature Control
The External Perfusion System for the Port-a-Patch is comprised of a computer controlled magnetic
pinch valve panel and a dedicated flow chamber. Up to eight different solutions can be handled
by the perfusion panel. Solutions can either be switched manually or via the software. The latterallows synchronization of data acquisition with solution exchange, as well as tagging of the collectedtraces with relevant information such as compound and concentration applied. Temperature
Control is also available in combination with the External Perfusion System enabling a stable
maintenance of elevated temperatures.

Ordner Number

ProductDetails

02 1001

External Perfusion

including:with Laminar Flow

- Valve Control Panel (with analog, digital and USB-port)

Chamber- Perfusion System starter kit with 50 ml syringes, Reagent
Kit, tubing, connectors, waste bottle etc.
- Stand with syringe holder- Laminar Perfusion Chamber- Manifold with accessories

03 1002

Temperatureincluding:Control without- Perfusion Canulla with heater and sensor

Perfusion system- Chamber Upgrade including resistive heating element- Temperature Control Software for on-the-fly visualizationof applied temperature2

Internal Perfusion
Nanion’s planar patch clamp chips offer the unique possibility to perfuse the intracellular side of themembrane. This aspect is exploited with the Internal Perfusion System for the Port-a-Patch. Up to eight
different solutions can be perfused inside the cell, allowing dose response analysis of compounds
acting on the cytosolic portion of the ion channel. Second messengers and metabolites can also beadded internally to investigate how ion channel function is affected. Solutions are exchanged within
seconds, and the ion channel modulation can be continuously monitored during the solution
exchange.

Ordner Number

ProductDetails

02 2001

Internal Perfusion

including:with Perfusion- Valve Control Panel (with analog and digital USB-ports)

system- Internal Perfusion Chamber with housing- Internal Perfusion starter kit: electrodes, Reagent Kit,
tubings and other accessories- Perfusion System starter kit with 50 ml syringes, Reagent
Kit, tubing, connectors, waste bottle etc.
- Stand with syringe holder

02 2002Internal Perfusion

including:

System w/o- Internal Perfusion Chamber with housing

Perfusion System- Internal Perfusion starter kit: electrodes, Reagent Kit,
tubings and other accessories* Only compatible with Nanion's Perfusion System3

Low Capacitance holder
The Low Capacitance Holder for the Port-a-Patch has been developed to reduce the capacitanceof the recording system, thereby increasing the temporal resolution and the signal-to-noise ratio. This
reduction in capacitance is critical for experiments requiring increased resolution, e.g. recordings ofsmall conductance ion channels with fast gating properties in a planar lipid bilayer or in the cellattached mode. The capacitance of the whole system including a bilayer on the chip isapproximatey 2.5 pF, compared to 6.5 pF for the standard Port-a-Patch. This corresponds to Irms
values of 400 fA compared to 1.1 pA (gain: 1000 mV/pA, bandwidth: 3 kHz) for the Low Capacitance
Holder and the standard Port-a-Patch.

Ordner Number

ProductDetails

01 1012Ultra-low noise add-including HEKA EPC-10 headstage

on for the Port-aPatch4

Microscope slide kit
Nanion offers the Port-a-Patch MicroscopeSlidedevelopedfor

thesimultaneous

visualization and patch clamp recording of thecell. Fluorescence measurements using anuprightmicroscope

cantherefore

becombined with current recordings.

Order Number

ProductDetails

01 1010Port-a-Patch Microscope Slide- 3 Port-a-Patch Microscope Slides

Kit- 1 Electrode set, adaptors and cabling

Port-a-Patch Microscope Slide

1 Port-a-Patch Microscope Slide

01 10115

Kits and spare parts
Order

ProductDetails

Port-a-Patch Spare Part Kit

2 NPC®-1 chip holders

Number08 1005

2 External electrodes
4 Internal electrodes
Silver wire
Bleach and sand paper
Spare tubing for Suction Control
08 1003

Port-a-patch Electrode Service

10 external electrodes

Kit

10 internal electrodes
Silver wire
Bleach and sand paper

08 1020

Electrode Service Kit for Low- 10 pairs of electrodes (internal andNoise Unit

external)- 10 o-rings- 1 tubing (length ˜0.5 m; silicone ID 2 mm,OD 4 mm)

02 1003

Laminar Flow Chamber Kit- Laminar Flow Perfusion Chamber- Manifold and accessories

05 1002

Vesicle Prep Pro Electrode Kit

1 pair of ITO slides and O-rings

08 1004

Technical Service Kit Perfusion- tubing set pinch valve

System- waste removal lid including tubing- spare part set: manifold, nozzle,manifold plugs, O-rings and vacuum
grease08 10133m silver wire

05 1004

VesiclePrepProFormation

Chamber6

Reagent Kits
OrderProductDetails

Reagent Kit Port-a-Patch

1x blue Cryo Box: 81 Vials:

standard

27x External standard, 18x Seal, 18x

Number08 1001

Internal K, 18x Internal Cs
08 1016

Reagent Kit Port-a-Patch to beSpecified by customer

specified by customer
08 1019

02 100308 1015

Reagent Kit Perfusion System

1x Rack (20x 50ml): 12x External standard,

standard, external

8x SealReagent Kit Perfusion System

1x Rack (20x 50ml): 10x Internal CS, 10xstandard, internalInternal K

Reagent Kit Perfusion System toSpecified by customer

be specified by customer
08 1006

Reagent Kit Vesicles for Port-a-

1 red cryo box: 81 vials

Patch

18x 200 mM KCl, pH 7, 18x 200 mM KCl,
pH 4, 18x 200 mM NaCl, pH 7,18x 200 mM
NaCl, pH 4, 7x 1M Sorbitol,1 mM EGTA, pH
7, 1x GUVs, 1x 100 mM HCl

05 1003

Vesicle Prep pro GUV Kit

5 vials x 500 µl GUVs (separatepreparations)
13 x 100 mM HCL, 9 x 1 M Sorbitol, 1 mM
EGTA, 2 mM Hepes/ KOH pH 7, 13 x 200
mM KCl, 10 mM Hepes/ KOH, pH 7, 14 x
200 mM KCl, 10 mM Hepes/ KOH, pH 47

NPC®1 chips
Standard chips for bilayer and patch clamp recordings. Minimum order 100 pieces.

Order Number

Product06 1101
06 1102
06 1103
06 1104
06 1105
06 1106

NPC®-1 patch clamp chips 1-2 MOhm
NPC®-1 patch clamp chips 2-3.5 MOhm
NPC®-1 patch clamp chips 3-5 MOhm
NPC®-1 patch clamp chips 5-10 MOhm
NPC®-1 patch clamp chips 8-12 MOhmNPC®-1 patch clamp chips 10-15 MOhm

8ServiceOrderProductDetails

Port-a-Patch Service Contract

Annually including:Number
08 2002

Complementary experimental support
Complementary PatchControl® updates
09 1002

Platform-Specific Training

Platform specific training with own choice(Beginner - Advanced Level) atof platform, add-ons, cells, assay by

Nanion's Premises

electrophysiologists at Nanion.
Charge per day

09 1003

Platform Specific Training at

Platform specific training with own choice

Customer's Premisesof platform, add-ons, cells and assay by
Nanionelectrophysiologist

onsitebycustomer.
Dailycharge+coverageoftravel

expenses
08 1027

Port-a-Patch, refurbishment

component: TO BE SPECIFIED
renewed warranty on refurbished parts: 6months9