GUIDELINE OF NGS SAMPLE PREPARATION & ORDER SUBMISSION
This document provides guidelines on how to prepare, quantify, and submit Next-Generation Sequencing (NGS) samples to Axil Scientific.
Whether you are submitting DNA or RNA sample, it is essential that the appropriate instructions be followed to enable the successful completion ofyour project.

I.

SAMPLE REQUIREMENTS

Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, we have extensive sample quality control
procedures to ensure submitted samples conform to requirements for downstream processing.

To guarantee the normal processing of your project, samples should meet the standards given below. If your samples do not meet these standards, or
if you are unable to produce higher-quality samples, please consult our Customer Care Department at ngs@axilscientific.com before shipping yoursamples.

Notes:
1. Input quantity should be determined by Qubit® instead of NanoDrop™, and the final quantity and concentration should conform to our
specifications.
2. Samples that do not meet these specifications will be classified as “sequencing at risk”, and will be subjected to full charges regardless of data
output. Please consult us for details.

1
1.1

Genome Sequencing
Human Whole Genome Sequencing
Library Type

Human Whole
Genome Library(350bp)Sample Type

Amount(Qubit®)VolumeConcentration

Purity(NanoDrop™ & Agarose Gel Assessment)

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

Genomic DNA(PCR-free)≥ 1.5 μg

≥ 20 μl≥ 20 ng/μl*FFPE DNA

≥ 800 ng--

Fragments should be longer than 1500 bp.

OD 260/280 = 1.8-2.0;
no degradation, no contamination.

*FFPE: Formalin-Fixed, Paraffin-Embedded.

1.2

Exome Sequencing/ Target Region Capture
Library TypeDNA Library(Human Exomeor Human Target
Region)Library Type

Mouse Exome
Library

Sample Type

Amount(Qubit®)VolumeConcentration

Purity(NanoDrop™ & Agarose Gel Assessment)

Genomic DNA≥ 400 ng

≥ 20 μl≥ 20 ng/μl

OD 260/280 = 1.8-2.0;
no degradation, no contamination.

*FFPE≥ 800 ng--

Fragments should be longer than 1000 bp.

cfDNA/ctDNA≥ 50 ng--

Fragments should be in multiples of 170 bp,
no genomic contamination.

Sample Type

Amount(Qubit®)VolumeConcentration

Purity(NanoDrop™ & Agarose Gel Assessment)

Genomic DNA≥ 400 ng

≥ 20 μl≥ 20 ng/μl

OD 260/280 = 1.8-2.0;
no degradation, no contamination.

*FFPE≥ 800 ng--

Fragments should be longer than 1000 bp.

*FFPE: Formalin-Fixed, Paraffin-Embedded.

1.3

Plant & Animal Genome Sequencing
Library Type

Plant and Animal
Whole Genome Library(≤ 500 bp)

1.4

Sample Type

Amount(Qubit®)VolumeConcentration

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

Genomic DNA(PCR-free non-350bp)≥ 5 μg≥ 20 μl≥ 30 ng/μl

Genomic DNA(PCR-free 350bp)≥ 1.5 μg

≥ 20 μl≥ 20 ng/μl

Purity(NanoDrop™ & Agarose Gel Assessment)

OD 260/280 = 1.8-2.0;
no degradation, no contamination.

Microbial Genome Sequencing
Library Type

Sample Type

Amount(Qubit®)VolumeConcentration

Metagenomics Library

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

PCR Free Library(Amplicon)

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

Microbial Whole Genome
Library (350bp)

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

Microbial Whole Genome
Library (PCR-free 350bp)

Genomic DNA≥ 1.5 μg

≥ 20 μl≥ 20 ng/μl

Purity(NanoDrop™ & Agarose Gel Assessment)

OD 260/280 = 1.8-2.0;
no degradation, no contamination.

1.5

PCR Product Sequencing
Library Type

Sample Type

Amount(Qubit®)VolumeConcentration

PCR Free Library

PCR Product≥ 1.5 μg

≥ 20 μl≥ 50 ng/μl

Purity(NanoDrop™ & Agarose Gel Assessment)

OD 260/280 = 1.8-2.0;
no degradation, no contamination.
Library (with PCR)

1.6

PCR Product≥ 200 ng

≥ 20 μl≥ 10 ng/μl

Sample Type

Amount(Qubit®)VolumeConcentration

Purity(NanoDrop™ & Agarose Gel Assessment)*HMW Genomic DNA(Animal and Plant)≥ 8 μg≥ 50 μl≥ 80 ng/μl

Fragment size: Majority above 40k bp;
OD 260/280=1.8-2.0; OD 260/230=1.5-2.6;**Nc/Qc=0.95-3.00;

PacBio Sequencing
Library TypePacBio Sequel
II DNA
CLR Library

PacBio Sequel
II DNA
Hifi Library*HMW Genomic DNA(Bacteria)≥ 5 μg≥ 50 μl≥ 80 ng/μl

Fragment size: Majority above 20k bp;
OD 260/280=1.8-2.0; OD 260/230=1.5-2.6;**Nc/Qc=0.95-3.00;*HMW Genomic DNA(Fungus)≥ 8 μg≥ 50 μl≥ 80 ng/μl

Fragment size: Majority above 20k bp;
OD 260/280=1.8-2.0; OD 260/230=1.5-2.6;**Nc/Qc=0.95-3.00;*HMW Genomic DNA≥ 15 μg≥ 50 μl≥ 80 ng/μl

Fragment size: Majority above 30k bp;
OD 260/280=1.8-2.0; OD 260/230=1.5-2.6;**Nc/Qc=0.95-3.00;*HMW: High Molecular Weight.
**Nc/Qc: NanoDrop™ concentration/Qubit concentration.

1.7

Nanopore Sequencing

Library Type

Amount(Qubit®)VolumeConcentration

DNA Integrity Number(Agilent 2200)

Purity(NanoDrop™ & Agarose Gel Assessment)*HMW Genomic DNA(Animal and Plant)≥ 10 μg≥ 100 μl

≥ 100 ng/μl≥ 7.5, with flat base line.

Fragment size: Majority above 30k bp;
OD 260/280=1.8-2.0; OD 260/230=1.5-2.6;**Nc/Qc=0.95-3.00;*HMW Genomic DNA(Bacteria and Fungus)≥ 10 μg≥ 100 μl

≥ 100 ng/μl≥ 7.5, with flat base line.

Fragment size: Majority above 20k bp;
OD 260/280=1.8-2.0; OD 260/230=1.5-2.6;**Nc/Qc=0.95-3.00;

16s Full Length
Amplicon Library

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

Not applicableOD 260/280=1.8-2.0; OD 260/230=1.5-2.6;
no degradation, no contamination.

Rapid Barcoding
Library

Plasmid DNA≥ 1 μg≥ 20 μl

Normalize to50 ng/μl

Not applicableOD 260/280=1.8-2.0; OD 260/230=1.5-2.6;
no degradation, no contamination.

Pooled Amplicons(≤ 1 kb)≥ 500 ng

≥ 50 μl≥ 10 ng/μl

Not applicableno degradation, no contamination.

Pooled Amplicons(≥ 1kb)≥ 1 μg≥ 100 μl

≥ 10 ng/μl

Not applicableno degradation, no contamination.

Amount(Qubit®)VolumeConcentration

Titration of the Targeted Virus(qPCR)

Total RNA Contains
SARS-CoV2 Virus

Not applicable≥ 40 μl

Not applicable≤ 25.0gDNA Contains
African Swine Flu Virus (ASFV)≥ 2 µg≥ 50 μl

Nanopore DNA
Library

Native Barcoding
Library

Sample Type

*HMW: High Molecular Weight.
**Nc/Qc: NanoDrop™ concentration/Qubit concentration.

1.8

Nanopore Virus Whole Genome Sequencing
Library Type

Rapid Barcoding or
Native Barcoding Library

Sample Type≥ 40 ng/μl≤ 25.02.

RNA Sequencing

2.1

Transcriptome Sequencing
Library TypeEukaryotic mRNA
Library(Poly A Enrichment)

Eukaryotic
Directional mRNA
Library(Poly A Enrichment)

Sample Type

Amount(Qubit®)VolumeConcentration

RNA Integrity Number(Agilent 2100)

Total RNA
(Animal)≥ 400 ng

≥ 20 μl≥ 20 ng/μl≥ 6.8, with flat base line.

Total RNA
(Plant and Fungus)≥ 400 ng

≥ 20 μl≥ 20 ng/μl≥ 6.3, with flat base line.

Total RNA(Blood)≥ 800 ng

≥ 20 μl≥ 20 ng/μl≥ 6.8, with flat base line.

Amplified cDNA
(double strand)≥ 100 ng

≥ 10 μl≥ 10 ng/μl

Fragments distributing
between 400bp-5000bp,
with the main peak at~2000bp;

Total RNA
(Animal)≥ 800 ng

≥ 20 μl≥ 20 ng/μl≥ 6.8, with flat base line.

Total RNA
(Plant and Fungus)≥ 800 ng

≥ 20 μl≥ 20 ng/μl≥ 6.3, with flat base line.

Total RNA(Blood)≥ 800 ng

≥ 20 μl≥ 20 ng/μl≥ 6.8, with flat base line.

Prokaryotic RNA
Library

Total RNA≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 6.0, with flat base line.

Meta-transcriptome
Library

Total RNA≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 6.5, with flat base line.

Purity(NanoDrop™ & Agarose Gel
Assessment)

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

OD 260/230 ≥ 2.0;
no degradation, no contamination.

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.
OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

2.2

Eukaryotic Small RNA Sequencing
Library Type

Sample Type

Small RNA
Library(with 18~40 bp
insert)Total RNA
(Animal)≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 7.5, with smooth baseline.

Total RNA
(Plant and Fungus)≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 7, with smooth base line.

-

Fragments distributing
between 25-200nt (byhigh sensitive Agilent 2100
Bioanalyzer), FU> 10, withno peak > 2000nt.

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

Purity(NanoDrop™ & Agarose Gel
Assessment)

Exosome RNA

2.3≥ 20 ng

VolumeConcentration

RNA Integrity Number(Agilent 2100)

Purity(NanoDrop™ & Agarose Gel
Assessment)

Amount(Qubit®)≥ 10 μl

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

Eukaryotic Long Non-coding RNA Sequencing
Library Type

Directional RNA
library(rRNA removal)

Sample Type

Amount(Qubit®)VolumeConcentration

RNA Integrity Number(Agilent 2100)

Total RNA
(Animal)≥ 500 ng

≥ 10 μl≥ 50 ng/μl≥ 6.5, flat base line.

Total RNA
(Plant and Fungus)≥ 500 ng

≥ 10 μl≥ 50 ng/μl≥ 6, flat base line.

-

Fragments distributing
between 25-200nt (byhigh sensitivity Agilent
2100 Bioanalyzer), FU> 10,with no peak > 2000nt.

Exosome RNA≥ 20 ng≥ 10 μl

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

2.4

Eukaryotic CircRNA Sequencing
Library Type

CircRNA Library

2.5

2.6

Sample Type

Amount(Qubit®)VolumeConcentration

RNA Integrity Number(Agilent 2100)

Total RNA
(Animal)≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 7, flat base line.

Total RNA
(Plant and Fungus)≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 6.5, flat base line.

Purity(NanoDrop™ & Agarose Gel
Assessment)
OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

Ribosome Profiling
Library Type

Sample Type

AmountVolumeConcentration

Main Peak

Purity(Agarose Gel Assessment)

Ribo Library

RPF Sample≥ 2 μg≥ 10 μl≥ 200 ng/μl

25-38 nt

No degradation, no contamination.

Concentration

RNA Integrity Number(Agilent 2100)

Purity(NanoDrop™ & Agarose Gel
Assessment)≥ 7, flat base line.

OD 260/280=1.8-2.2;
OD 260/230=1.3-2.5;*Nc/Qc ≤ 2.5;

PacBio Iso-seq
Library TypePacBio Sequel II
RNA library

Sample Type

Total RNA

Amount≥ 2 μg*Nc/Qc: NanoDrop concentration/Qubit concentration

Volume≥ 50 μl≥ 30 ng/μl

2.7

Nanopore Direct RNA Sequencing
Sample Type

AmountVolumeConcentration

RNA Integrity Number(Agilent 2100)

Purity(NanoDrop™ & Agarose Gel Assessment)

Total RNA
(Eukaryotes)≥ 2 μg≥ 20 μl≥ 50 ng/μl≥ 7, flat base line.

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

Poly(A)-tailed RNA(Prokaryotes)≥ 200 ng

≥ 20 μl≥ 20 ng/μl≥ 7, flat base line.

OD 260/280 ≥ 2.0;
OD 260/230 ≥ 2.0;
no degradation, no contamination.

Library Type

Direct RNA
Library3.

Epigenetics Sequencing
Library Type

Sample Type

AmountVolumeConcentration

Purity(NanoDrop™ & Agarose Gel Assessment)

Whole Genome
Methylation (WGBS) Library

Genomic DNA≥ 200 ng

≥ 20 μl≥ 10 ng/μl

0 < OD260/230 < 3;
no degradation, no contamination.

Reduced Representation
Bisulfite Sequencing (RRBS)
Library

Genomic DNA≥ 1.5 μg

≥ 20 μl≥ 20 ng/μl

0＜OD260/230＜3;no degradation, no contamination.

ChIP-seq Library

Enriched DNA≥ 50 ng≥ 20 μl≥ 2 ng/μl

Main peak of 100 bp-500 bp.

RIP-seq Library

Enriched DNA≥ 100 ng

≥ 20 μl≥ 3 ng/μl

Without fragmentation;
fragments should be longer than 1000 bp.

4.

Pre-made Library Sequencing

4.1

Volume Requirement

PE150-HiSeq
Data Amount

Volume Requirement*

Lane Sequencing≥ 20 μl (additional 10 μl for one more lane).

Data Amount

Volume Requirement*＜20 Gb≥ 15 μl

20 Gb ≤ X ≤ 100 Gb≥ 25 μl

100 Gb < X < 400 Gb≥ 50 μl

Lane Sequencing≥ 50 μl (additional 40 μl for one more lane).

PE150-NovaSeq

NovaSeq PE250 & SE50 & PE50
Data Amount

Volume Requirement*

X ≤ 20M reads≥ 15 μl

20M reads＜X ≤ 50M reads≥ 25 μl

50M reads＜X＜150M reads*≥ 50 μl

Lane Sequencing≥ 100 μl (additional 100 μl for one more lane).

*Notes:High concentration libraries should be diluted before shipping.
Pre-made libraries should be colorless.

4.2

Library Concentration≥ 0.5 ng/μL, quantified by Qubit® 2.0 (Life Technologies).
2 nM-30 nM, quantified by qPCR.

4.3

Library Size
Library size: insert + adapters (120 bp) ± 50 bp (this size estimation does not apply to small RNA library).
Single main peak, no multiple peaks, no adapter contamination, and no primer dimers.
Sequencing Strategy
SE 50/ PE 50
PE 150PE 250Lane Sequencing

Library Size (insert + adaptors (120 bp)+/- 50 bp for Optimal Results
130 bp~650 bp
320 bp~520 bp
400 bp~650 bp≥ 100 μl (additional 100 μl for one more lane).

II. OVERVIEW OF SAMPLE QUALITY CONTROL (QC)
Before order submission, we encourage customers to examine the sample quality using one of the following methods: Qubit®, NanoDrop™, agarose
gel electrophoresis, or Agilent 2100.
We recommend samples to go through a quick check using Qubit®/ PicoGreen® / Agarose gel electrophoresis (with quantity indicator), so thatthe result will correspond more closely to our QC results.
If NanoDrop™ is used to estimate the sample quantity before order submission, we strongly recommend that you send more DNA/ RNA forprocessing than the amount given above.
For gel electrophoresis, the following conditions are recommended:
DNA: 1.0% agarose gel; 1x TAE buffer; 100V for 60 min
RNA: 1.0% agarose gel; 1x TAE buffer; 100V for 60 min

Note:
Different electrophoresis conditions may generate a different and potentially misleading QC report on your samples. Therefore, before ordersubmission, it is highly recommended that you provide us the agarose gel photo of your samples by adhering to the conditions recommended above.

III. EXAMPLE OF QUALIFIED DNA & RNA SAMPLES
1.

Information of DNA Markers

The following molecular size DNA markers (in bp) were used in our original sample QC.

Fig. 1. (A) Trans2K™ Plus DNA Marker; (B) λ/HindIII DNA Marker; (C) 1st BASE 100bp DNA Marker; (D) 1st BASE 1kb DNA Marker

2.

Example of Qualified DNA Samples

2.1

Comparison of Qualified DNA Sample

A qualified DNA sample is compared with some common types of unqualified DNA samples (Fig. 2):

Fig. 2. Comparison of DNA sample quality. Red boxes denote areas of contamination.
(A)

Trans2K™ Plus DNA Marker;(B)

Qualified DNA Sample;(C)

Degraded DNA Sample;(D)

DNA Sample contaminated with RNA;(E)DNA Sample contaminated with protein.

2.2

DNA Samples with Degradation

The gel picture illustrates DNA samples with degradation. Severe degradation can impact the quality of the prepared library and subsequentbioinformatics analysis (Fig. 3):

Fig. 3. DNA samples with degradation. Panels A, B, and C demonstrate increasing levels of DNA degradation. M-1, Trans2K™ Plus DNA Marker.

2.3 DNA Samples with RNA Contamination
RNA contamination of DNA samples (Fig. 4) can impede the library construction process. It is strongly recommended to clean your DNA samples
with RNase during the process of DNA extraction.

Fig. 4. DNA samples contaminated with RNA. Panels A – D demonstrate increasing levels of RNA degradation. Red boxes denote areas ofcontamination. M-1 = Trans2K™ Plus DNA Marker.

2.4

DNA Samples with Protein Contamination

DNA samples can be contaminated by proteins (Fig. 5) during the process of DNA extraction from raw materials. The protein-contaminated DNA
samples can be purified using affinity column, though it will lead to some loss of DNA.

Fig. 5. DNA samples contaminated with protein. Panels A – C demonstrate increasing levels of protein contamination. Red boxes denote areasof contamination. M-1, Trans2K™ Plus DNA Marker.

3.

Example of Qualified RNA Samples

3.1

Comparison of Qualified RNA Sample
A qualified RNA sample is compared with some common types of unqualified RNA samples (Fig. 6):

Fig. 6. Comparison of RNA sample quality. Red boxes denote areas of contamination. DNA Marker = Trans2K™ Plus DNA Marker.
(A) Qualified RNA sample;(B) RNA Sample with protein contamination;(C) RNA Samples with degradation;(D) RNA samples with genomic DNA contamination.

3.2

RNA Samples with Protein Contamination

Fig. 7. RNA samples with protein contamination. Red boxes denote areas of contamination. DNA Marker = Trans2K™ Plus DNA Marker.
Panels A to D demonstrate increasing levels of protein contamination.

3.3

Comparison of RNA samples on Agarose Gel and Agilent 2100 Analysis

Fig. 8. An example of gel electrophoresis (left),and Agilent 2100 results (right), for a qualified
RNA sample.

Fig. 9. An example of gel electrophoresis (left), and Agilent
2100 results (right), for a degraded RNA sample.

Fig. 10. An example of gel electrophoresis (left), and Agilent

Fig. 11. An example of gel electrophoresis (left), and2100 results (right), for a RNA sample with solvent/ salt

Agilent 2100 results (right), for a RNA sample withcontamination (high viscosity).

protein contamination. The RNA Labchip would notable to show DNA contamination.

IV. LABELING OF YOUR SAMPLE TUBES
1. It is important to have a good labelling on sample tubes. The label on tubes must always tally with the sample name indicated in the order form. Usewaterproof permanent marker pen for labelling to keep labels from fading upon receipt. You should label on both sides of the tube lid and its tube body.
2. According to the tube label, tally match the sample name in the provided NGS Order Form before submission.

V. PACKING OF YOUR ORDER
1. For DNA and RNA samples, use 1.5 mL or 2.0 mL screw-cap DNase and RNase-free micro-centrifuge tubes. Use Parafilm to seal each tube
before packaging. We do not recommend shipping samples in organic solvents (such as absolute ethanol or isopropanol) because the solvents willleak during shipping, which could cause cross-contamination between samples, as well as losing sample from further process.
2. In order to minimize the micro-centrifuge tubes from damage during shipping, we recommend placing the sample tubes in a container, such as a
50-ml Falcon tube. Cotton/ absorbent papers/ dunnage can be used to prevent tubes from moving around inside the container.
3. We recommend dry ice shipment for majority of NGS samples. Genomic DNA is optional to ship with blue ice (4°C). Saliva samples that require
DNA extraction services before sequencing must be shipped at room temperature.
4. 96-well plate and PCR strips tubes are NOT accepted for shipping (See picture below).

Fig. 12. Recommended and prohibited
tubes for sending samples.

5. Any raw materials that require DNA/ RNA extraction services before sequencing, please confirm with us the sample submission method (e.g. amountand size of raw materials, replicates and shipping temperature) before arranging the shipment to us. Other information can be found athttps://base-asia.com/downloads/products/NGS/Sample-Submission-Guidelines-gDNA-RNA-Extraction.pdf

VI. COMPLETING THE ORDER FORM
✓ Before submitting the order, the ordering information must be completed using NGS Order Form.
✓ Send us the ELECTRONIC COPY of the completed order form via email.
✓ Kindly label your NGS Project ID on the parcel or the Ziplock bag that secures your samples.
✓ Remember to provide a copy of gel photo or Bioanalyzer trace if available.

VII. SUBMITTING THE ORDER
1. Ensure all samples follow our quality standards and they are prepared and packaged according to the guidelines given above.
2. Before submitting and ship the order, notify us at ngs@axilscientific.com and attached the required order form and gel photo.
3. Sample transportation options:

DNA

RNA

1.

Lyophilize the DNA to ship on ambient temperature

2.

Pack with ice packs/blue ice (2-8 °C) *Recommended3.

Use the cold-chain transportation system (2-8 °C) of the courier

4.

DNAstable (Liquid format, Biomatrica) and ship on 2-8 °C

5.

Pack in dry ice (-60 °C – -80 °C)

1.

Lyophilize the RNA to ship on dry ice

2.

Suspend RNA in 75% ethanol and ship on dry ice

3.

RNAstable (Biomatrica) and ship on 2-8 °C

4.

Pack in dry ice (-60 °C – -80 °C) *Recommended

4. Email NGS Order Form to ngs@axilscientific.com before ship or submit the order.
5. Once the parcels reach our facility, all samples will be stored in -80°C ultra-low temperature freezer before QC. Our Customer Care Specialist
will provide a timely update to you about the progress of your order.

Qubit is a trademark of Life Technologies and Thermo Fisher Scientific.
NanoDrop is a trademark of NanoDrop Technologies LLC.
Agilent 2100 Bioanalyzer or Agilent 2200 TapeStation is a trademark of Agilent Technologies.
Trans2K Plus is a trademark of TransGen Biotech.
1st BASE is a trademark of Axil Scientific Pte. Ltd.