GMP Human Flt-3 Ligand Protein
Catalog # GMP-FLLH28

Features

Sterility

Designed under ISO 9001:2015 and ISO 13485:2016
Manufactured and QC tested under a GMP compliance factory
Animal-Free materials
Beta-lactam materials freeThe sterility testing was performed by membrane filtration method described in
CP<1101>, USP<71> and Eur. Ph. 2.6.1.

Mycoplasma
Negative.

PurityBatch-to-batch consistency
Stringent quality control tests>95% as determined by SDS-PAGE.

Formulation

SourceGMP Human Flt-3 Ligand Protein(GMP-FLLH28) is expressed from human 293
cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1).
Predicted N-terminus: Thr 27

Molecular Characterization

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.

Shipping
This product is supplied and shipped with blue ice, please inquire the shipping
cost.

Storage
This protein carries no "tag".
The protein has a calculated MW of 18.0 kDa. The protein migrates as 24 kDa &27 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:Endotoxin-20°C to -70°C for 5 years in lyophilized state;Less than 10 EU/mg by the LAL method.

-70°C for 12 months under sterile conditions after reconstitution.

Host Cell Protein<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA<0.02 ng/μg of protein tested by qPCR.

SDS-PAGE

GMP Human Flt-3 Ligand Protein on SDS-PAGE under reducing (R)condition. The gel was stained with Coomassie Blue. The purity of the protein
is greater than 95%.

www.acrobiosystems.com

9/18/2024

GMP Human Flt-3 Ligand Protein
Catalog # GMP-FLLH28

Bioactivity-Bioactivity CELL BASE

GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28) stimulates

The activity of GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28)

proliferation of OCI-AML5 cells. The specific activity of GMP Human Flt-3

was higher than other competing products.

Ligand Protein is > 5.00 x 10^5 IU/mg, which is calibrated against WHO
Reference Reagent FMS-LIKE TYROSINE KINASE 3 LIGAND (NIBSC
code: 96/532) (QC tested).

Bioactivity-Stability

The Cell based assay shows that GMP Human Flt-3 Ligand Protein (Cat. No.

The Cell based assay shows that GMP Human Flt-3 Ligand Protein (Cat. No.

GMP-FLLH28) is stable at 37°C for 24 hours.

GMP-FLLH28) is stable after freezing and thawing 3 times.

The Cell based assay shows that GMP Human Flt-3 Ligand Protein (Cat. No.
GMP-FLLH28) is stable at 4℃ for 180 days.

www.acrobiosystems.com

9/18/2024

GMP Human Flt-3 Ligand Protein
Catalog # GMP-FLLH28

The Cell based assay shows batch-to-batch consistency between Acro's GMP
and PG Flt-3 Ligand.

MANUFACTURING SPECIFICATIONSACROBiosystems GMP grade products are produced under a quality management system and in compliance with relevant guidelines: Ph. Eur General Chapter
5.2.12 Raw materials of biological origin for the production of cell-based and gene therapy medicinal products; USP<92>Growth Factors and Cytokines Used in Cell
Therapy Manufacturing; USP<1043>Ancillary Materials for Cell, Gene, and Tissue-Engineered Products; ISO/TS 20399-1:2018, Biotechnology - Ancillary
Materials Present During the Production of Cellular Therapeutic Products.

ACROBiosystems Quality Management System Contents：
Designed under ISO 9001:2015 and ISO 13485:2016, Manufactured and QC tested under a GMP compliance factory.
Animal-Free materials
Materials purchased from the approved suppliers by QA
ISO 5 clean rooms and automatic filling equipment
Qualified personnel
Quality-related documents review and approve by QA
Fully batch production and control records
Equipment maintenance and calibration
Validation of analytical procedures
Stability studies conducted
Comprehensive regulatory support files
Request For Regulatory Support Files（RSF）

ACROBiosystems provide rigorous quality control tests (fully validated equipment, processes and test methods) on our GMP grade products to ensure that they meetstringent standards in terms of purity, safety, activity and inter-batch stability, and each bulk QC lot mainly contains the following specific information:
SDS-PAGE
Protein content
Endotoxin level
Residual Host Cell DNA content
Residual Host Cell Protein content
Biological activity analysis
Microbial testing
Mycoplasma testingIn vitro virus assay
Residual moisture
Batch-to-batch consistencywww.acrobiosystems.com

9/18/2024

GMP Human Flt-3 Ligand Protein
Catalog # GMP-FLLH28

BackgroundFMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple
hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries
approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1).
FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of thehematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immaturedendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 andIL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG iscrucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

Clinical and Translational Updates

www.acrobiosystems.com

9/18/2024