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Cell Analytics
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Accelerate
your research[Cell Analytics

Next level live-cell kinetic andphenotypic assays
AtlaZ
AtlaZ accelerates cellular research by enabling theinvestigation of a large variety of effects in cells overtime. It offers label-free and real-time monitoring
capabilities. It can simultaneously or independently
record data from up to six 96-well plates.
To obtain a comprehensive understanding ofyour cells, it is essential to employ continuous andreal-time monitoring, which provides access tokinetic and phenotypic information. The system's
methodology relies on electrical impedance
spectroscopy, which coupled with its impressive
throughput, delivers an unprecedented quantityand quality of information from your cellularexperiments.
When the cells adhere, proliferate or die, they
gradually prevent or enable the flow of electric
current through the electrodes integrated in theculture plate, leading to a change in impedance.
It has been proven to be an excellent method formonitoring cell adhesion, cell specific structuralchanges, GPCR research, cancer research (e.g.
breast cancer, immuno-oncology), proliferation andcytotoxicity (e.g. cardiotoxicity or hepatoxicity).

compound-induced effectcell detachment

Cell Signal

cell confluency

loss of tight junctions
loss of membrane integrity

cell proliferation
cell adhesion

Time (h)Electrode3

Electric current

Suspended cell

Adherent cell

AtlaZ

Choose your quantitativecell analytics solution:• Record from 6 x 96 well plates simultaneously or independently in real-time and label-free• Select various recording modes for each plate.
• Cytolysis Mode allows for investigations into cell-killing kinetics.
• Cell Monitoring Mode facilitates the assessment of GPCR distal effects.
• TEER signal Mode enables the examination of barrier integrity.
• Unique Cell Signal at all available frequencies (full spectrum from 0.1 kHz - 100 kHz) allows thedetection of a multitude of physiological effects in cells.

AtlaZ offers simplicity
you will love

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Cell Analytics

Sophisticated solutions
for cardiac research
CardioExcyte 96
Fundamental insights on the electrical andmechanical dynamics of the human heart canuncover the complexity of cardiac disease.
CardioExcyte 96 offers a unique experimental
constellation, uniting electrical and impedance
readouts from the same cells, such as human iPSC
derived cardiomyocytes. As a hybrid system it gives
valuable insights into both electrophysiologicaland mechanical cellular phenotypes. In addition,
the base impedance readout is continuously andautomatically monitored as a measure of acute andchronic cell activity.

No subtleties of cytotoxic responses are missed,
this includes non-contractile cell types such as
hepatocyte-like cells or cancer cells as wellas contractile cardiac cells. Impedance andextracellular field potential measurements are
performed at high resolution, are non-invasive, andlabel-free. The CardioExcyte 96 is an automateddevice, recording from 96 wells at a time.
Standardized recording and analysis protocols for
CardioExcyte 96 recordings are derived from theefforts of the Comprehensive In vitro Proarrythmia
Assay (CiPA) myocyte working group.

Electrophysiology

Impedance

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Cell analytics

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Combine contractility,
electrophysiology,
and cell viability inone instrument.

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Cell Analytics

Contractility and maturation underphysiological conditions
FLEXcyte 96
The FLEXcyte 96 provides a unique andadvantageous physiological environment forstudying cardiac cells, free from labeling or artificialinterventions. This innovative technology greatly
enhances the evaluation of drug candidates in
cardiac safety, tox, and efficacy studies or basicresearch.
The system transforms traditionally limited cardiac
contractility approaches of the Langendorff Heart
into a cutting-edge modern high-throughput
technique operating on a unique flexible substrate,
mimicking an in vivo-like environment. It enables

Langendorff Heart
Adult cardiac phenotype

Target species

Throughput

Measured parameters

Genetic disease model

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real-time measurements of cardiac contractility,
allowing researchers to observe and analyze thedynamic changes in cell behavior.
Additionally, the system accurately quantifies
the force-frequency relation, providing valuable
insights into the relationship between contractile
force and heart rate. Furthermore, it is capable ofdetecting inotropic (changes in contractile strength)
and chronotropic (changes in heart rate) effects,
facilitating comprehensive investigations into theeffects of various compounds or interventions on
cardiac function.

FLEXcyte 96

FLEXcyte 96

Long-term recordings
can be performed ona lab benchtop, as theincubation system takes
care of the optimal
temperature, CO2 gas mix,
and humidity. The system´soptional hypoxia variation,
enables additional
regulation of N2 levels.

FLEXcyte 96 uses aflexible, promaturation
substrate for recording
cardiac cells. This unique
technology allows forrecordings in a nativelike environment close tomechanical conditions ofthe heart.

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1450

121040830

620

41020002040
Days in culture

Contractile force (mN/mm2)

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Beat rate (bpm)Mature cardiac
phenotype
Flexibility is key for a pro-maturation
effect on cultured human iPSCderived cardiomyocytes. TheFLEXcyte 96 system provides bothbasic prerequisites needed to record
mature cardiac force (mN/mm2).

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Cell analytics

Throughput, flexibility, pro-maturation

Throughput is essential when working with precious
materials, such as patient cells or when running
multiple drug assays. The CardioExcyte 96 andFLEXcyte 96 use standard format 96-well plates
in various configurations to ensure optimal
experimental flexibility, data reliability and speed.
In addition, AtlaZ offers an option to record from 6 x
96 well plates, thereby significantly increasing dataoutput.

9Flexibility is key for a pro-maturation effect on
cultured human iPSC-derived cardiomyocytes.
The flexible, native like environment mimics
the mechanical conditions of real biologicaltissue thereby inducing pro-maturation effects.
Additionally, electrical, optical and pressure
stimulation options for the CardioExcyte 96 or theFLEXcyte 96 offer the perfect tool for contractility
research in the pre-clinical field.

Cell analytics

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How well do
you know
your cells?

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“We recently purchased the CardioExcyte 96 / FLEXcyte 96 system throughthe NIH shared instrument grant (S10) program to be used by a diverse groupof researchers who use cultured heart cells to study genetic and other cardiac
disorders or who need to test lead compounds for cardiac toxicity. We havebeen thoroughly satisfied by the performance of the instrument and havebeen pleased with the great support from Nanion. The 96-well format enables
critically important replication and the ability to conduct multiple simultaneousexperiments. We are now exploring use of the instrument to interrogate othertypes of contractile cells. Given the enthusiasm from multiple investigators, weare going to purchase a second instrument.
Prof. Al George, Northwestern University, Chicago, IL, USA“Electrical Impedance Spectroscopy will become just as relevant for adherent
cells as flow cytometry is relevant for cells in suspension. The methodology
promises a huge potential for future scientific discoveries.”
Prof. Dr. Joachim Wegener, University of Regensburg“My experience with Nanion´s technology was great, achieving highly
reproducible data with the AtlaZ platform from day 1 wasconvincing for me.”Prof. Dr. Percy Knolle, Technical University of Munich (TUM)“ Human iPSC-derived cardiomyocytes are the future of safe and efficient
drug development. The FLEXcyte technology provides a tool for functional
cardiac toxicology and efficacy screening on these cells. Force-frequency
relation are the subject of current scientific discussions and require an assay
technology that can accurately quantify them and we meet this need with
the FLEXcyte 96.”
Peter Linder, CTO, Innovitro (CRO, FLEXcyte service provider)11

accelerate your researchnanion Europe
info@nanion.dephone: +49 89 219 095 0
nanion USA
info@naniontech.com
phone: 1-888-9-NANION
nanion China
andy.di@nanion.cn
phone: +86 10 82 17 6388
nanion Japan
info@nanion.jp
phone: +81 3 6457 8773
www.nanion.de

To find your nearest Nanion
representative, visit our website:www.nanion.de