Anti- CPE (Clostridium perfringens Enterotoxin) antibody, rabbit polyclonal
Product code

64-052Size

200 µgStorage-20℃

Concentration

4.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenHighly purified enterotoxin from Clostridium perfringens, strain NCTC8239(BioAcademia 01-509)

IsotypeRabbit IgG

Reactivity

Clostridium perfringens enterotoxin.(food-poisoning strains)

Special notes

N/A

Application

1. Western blot (1/1,000-1/2,000)
2. Dot blot (1/20,000- 1/100,000)
3. Immunoprecipitation (1/200-1/500)
4. ELISA (1/1,000)

Background

Clostridium perfringens enterotoxin (CPE) is a protein toxin produced bymajority of C. perfringens strains (type A, C, D and E strains) responsible forfood poisoning and gastrointestinal disease. CPE destroys cell membrane
structure of animals by its phospholipase activity after binding to the membraneof Claudin family proteins, which are components of tight junction of epithelial
cell membrane. CPE binds to Claudins 3, 4, 6, 7, 8 and 14, but not to Claudins
1, 2, 5 and 10.
For detail, refer toFujita K et al “Clostridium perfringens enterotoxin binds to the second
extracellular loop of claudin-3,a tight junction integral membrane protein.”

FEBS Lett 476: 258-261 (2000) PMID: 10913624

Data Link

UniProtKB P01558 (ELTB_CLOPE)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 64-052 Anti- CPE (Clostridium perfringens Enterotoxin) antibody, rabbit polyclonal

Fig.1 Western Blot of CPE in the culture supernatant of C. perfringens .
The proteins in the sample were separated on 12.5% SDS-PAGE.
Blotted at 15V, overnight in wet system, on PVDF membrane.
Blocked by shaking with 5% skim milk 1 h and washed with TBST buffer.
Anti-CPE antibody was used at 1µg/ml and incubated at room temperature for 1 h. The membrane
was incubated in TBST buffer for 5 min and washing was done 3 times with the same buffer.
Second antibody, goat anti-rabbit IgG H & L (HRP) (ab97051) was used at 1/20,000 and incubated for
1 h at room temperature. Washing was done 3 times as before with TBST buffer.
Visualized with BioRad ECL system.

Fig.2 Dot blot of CPE with anti-CPE antibody.
Indicated amounts of purified C.perfringence enterotoxin were spotted on nitrocellulose membraneand reacted with the indicated concentrations of anti-CPE antibody for 60 min. As the second antibody,goat anti-rabit IgG H & L (HRP) (ab97051) was reacted at 1/20,000 dilution for 60 min.
2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

ELISA data
3.000
2.500

Ｏ．Ｄ．

2.000
1.500
1.000
0.500
0.000
1000

2000

4000

8000

16000

32000

64000

DilutionFig.3. Titration of the anti-CPE antibody activity of the antibody by Indirect ELISA
Plate was coated with 0.2 μg of C.perfringens Enterotoxin (#01-509) per well and 100 μl of anti-C.perfringens Enterotoxin antibody at the indicated dilution was added to each well and incubated.
After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Colorwas developed with TMB as substrate. After washing, goat anti-rabbit-IgG conjugated with HRP(ab97051) was added as a secondary antibody. Color was developed with TMB as substrate. Opticaldensity (O.D.) was measures at 490 nm.

Fig.4. Titration of enterotoxin of C. perfringens by indirect ELISA using th anti-CPE antibody.
ELISA plate was coated with 100 μl of indicated concentration of C. perfringens enterotoxin per well.
The anti-CPE antibody was used at 1/1,000 dilution. ELISA was performed as in Fig.2.
3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/