GMP Human VEGF165 Protein
Catalog # GMP-VE5H23

Features

Sterility

Designed under ISO 9001:2015 and ISO 13485:2016
Manufactured and QC tested under a GMP compliance factory
Animal-Free materials
Beta-lactam materials free

The sterility testing was performed by membrane filtration method described in
CP<1101>, USP<71> and Eur. Ph. 2.6.1.

Mycoplasma
Negative.

PurityBatch-to-batch consistency
Stringent quality control tests>95% as determined by SDS-PAGE.

Formulation

SourceGMP Human VEGF165 Protein(GMP-VE5H23) is expressed from human 293
cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
Predicted N-terminus: Ala 27

Molecular Characterization

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.

Shipping
This product is supplied and shipped with blue ice, please inquire the shipping
cost.

Storage
This protein carries no "tag".
The protein has a calculated MW of 19.2 kDa. The protein migrates as 24 kDa±3
kDa when calibrated against Star Ribbon Pre-stained Protein Marker under
reducing (R) condition (SDS-PAGE) due to glycosylation.

Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:-20°C to -70°C for 5 years in lyophilized state;

Endotoxin-70°C for 12 months under sterile conditions after reconstitution.

Less than 10 EU/mg by the LAL method.

Host Cell Protein<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA<0.02 ng/μg of protein tested by qPCR.

SDS-PAGE

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9/18/2024

GMP Human VEGF165 Protein
Catalog # GMP-VE5H23

GMP Human VEGF165 Protein on SDS-PAGE under reducing (R) condition.
The gel was stained with Coomassie Blue. The purity of the protein is greaterthan 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-Bioactivity CELL BASE

GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) stimulates

The activity of GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) wasproliferation of 293F-NFAT/Luc-VEGFR2-3-7 cells. The specific activity ofhigher than other competing products.

GMP Human VEGF165 Protein is＞8.00 X 10^5 IU/mg, which is calibrated
against human vascular endothelial growth factor 165 WHO International
Standard (NIBSC code: 02/286) (QC tested).

Bioactivity-Stability

The Cell based assay shows that GMP Human VEGF165 Protein (Cat. No.

The Cell based assay shows that GMP Human VEGF165 Protein (Cat. No.

GMP-VE5H23) is stable at 37°C for 24 hours.

GMP-VE5H23) is stable after freezing and thawing 3 times.

www.acrobiosystems.com

9/18/2024

GMP Human VEGF165 Protein
Catalog # GMP-VE5H23

The Cell based assay shows that GMP Human VEGF165 Protein (Cat. No.
GMP-VE5H23) is stable at 4℃ for 180 days.

The Cell based assay shows batch-to-batch consistency between Acro's GMP
and PG VEGF165.

MANUFACTURING SPECIFICATIONSACROBiosystems GMP grade products are produced under a quality management system and in compliance with relevant guidelines: Ph. Eur General Chapter
5.2.12 Raw materials of biological origin for the production of cell-based and gene therapy medicinal products; USP<92>Growth Factors and Cytokines Used in Cell
Therapy Manufacturing; USP<1043>Ancillary Materials for Cell, Gene, and Tissue-Engineered Products; ISO/TS 20399-1:2018, Biotechnology - Ancillary
Materials Present During the Production of Cellular Therapeutic Products.

ACROBiosystems Quality Management System Contents：
Designed under ISO 9001:2015 and ISO 13485:2016, Manufactured and QC tested under a GMP compliance factory.
Animal-Free materials
Materials purchased from the approved suppliers by QA
ISO 5 clean rooms and automatic filling equipment
Qualified personnel
Quality-related documents review and approve by QA
Fully batch production and control records
Equipment maintenance and calibration
Validation of analytical procedures
Stability studies conducted
Comprehensive regulatory support files
Request For Regulatory Support Files（RSF）

ACROBiosystems provide rigorous quality control tests (fully validated equipment, processes and test methods) on our GMP grade products to ensure that they meetstringent standards in terms of purity, safety, activity and inter-batch stability, and each bulk QC lot mainly contains the following specific information:
SDS-PAGE
Protein content
Endotoxin level

www.acrobiosystems.com

9/18/2024

GMP Human VEGF165 Protein
Catalog # GMP-VE5H23

Residual Host Cell DNA content
Residual Host Cell Protein content
Biological activity analysis
Microbial testing
Mycoplasma testingIn vitro virus assay
Residual moisture
Batch-to-batch consistency

Background
VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells.
VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia,
inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to thereceptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance
VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role intumor angiogenesis in many cancers.

Clinical and Translational Updates

www.acrobiosystems.com

9/18/2024