Anti-Vero Toxin / Shiga Toxin subunit A antibody, mouse monoclonal (vero-01)
Product code64-027Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium

Immunogen

Culture supernatant of E. coli 0111 ( EHEC strain)

Isotype

Mouse IgG1

Reactivity

Reacts with subunit A of VT1, VT2 and Stx

Special notes

N/AApplication

1. Western blotting (1/500~1/1,000 )2. ELISA (assay dependent)
3. Immunochromatography (assay dependent)
Other applications have not been tested.

Background

Vero toxin (VT) is produced by Enterohaemorrhagic strains of Escherichia coli
(E. coli, VTEC, EHEC) and has lethal activity to Vero cells. EHEC strains
produce either one or both of the VT1 and VT2. Both VT1 and VT2 consist of one
subunit A and five copies of subunit B.

The primary structure of VT1 isidentical or nearly identical to Shiga toxin (Stx) produced by Shigelladysenteriae serotype 1 and also called Slt 1 (Shiga-like toxin 1). Subunit A
causes inhibition of protein synthesis and subsequent cell death, whereas
subunit B is presumed to bind specifically to the Vero cell-surface receptor andmediate uptake of the toxins. Some E. coli strains produce both Slt1 and Slt2,
and they share sequence identity of 55 %, but they are immunologically distinct.

Data Link

UniProtKB P08026 (Shiga-like toxin 1 A),
UniProtKB P09385 (Shiga-like toxin 2 A), Q9FBI2 (Shiga toxin A)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 64-027 Anti-Vero Toxin / Shiga Toxin subunit A antibody, mouse monoclonal (vero-01)

Fig.1. Detection of VT1 and VT2 by Western blotting with monoclonal antibody (vero-01).
1.

Culture medium of E. coli O157:H7

2.

Purified VT1

3.

Purified VT2

4.

VT non-producing E. coli.

Arrow shows subunit A (32kDa) of VT1 and VT2.

Fig

2.

Reactivity of the antibody (vero-1) with various food poisoning bacteria in

immunochromatographic strip test.
Rabbit anti-VT/Stx serum (BioAcademia, 64-025) was coated onto a specific area (test line) of anitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (controlline) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with themonoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted.
(1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6)Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains,
O26, O111 and O157:H7, but not with other food poisoning bacteria.
OLE1 migrated slightly faster than the predicted mass of 18.6 kD.
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Fig.3. Titration of antibody reactivity of the monoclonal antibody by indirect ELISA using crude
extract of E.coli O157:H7
The wells of plate were coated with crude extract of E. coli O157:H7 (100 µl, 1 μg/ml). After blocking
with 5% skim milk, 100 µl of antibody at the indicated dilution was added to the each well. HRPconjugate goat anti-mouse IgG (100μl、x2000 dilution) was added. Color was developed with OPD(orthophenylenediamine) as substrate. Optical densities (OD) measured at 490nm.

Reference: There has been no publication using this antibody. Please let us know when yourresearch using this antibody is published. We will offer one vial of our antibody as compliment.

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/