GMP Human VCAM-1 Protein, Fc Tag
Catalog # GMP-VC1H25

Features

Sterility

Designed under ISO 9001:2015 and ISO 13485:2016
Manufactured and QC tested under a GMP compliance factory
Animal-Free materials
Beta-lactam materials free

The sterility testing was performed by membrane filtration method described in
CP<1101>, USP<71> and Eur. Ph. 2.6.1.

Mycoplasma
Negative.

PurityBatch-to-batch consistency
Stringent quality control tests>95% as determined by SDS-PAGE.

Formulation

SourceGMP Human VCAM-1 Protein, Fc Tag(GMP-VC1H25) is expressed fromhuman 293 cells (HEK293). It contains AA Phe 25 - Glu 698 (Accession #P19320-1).

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.

Predicted N-terminus: Phe 25Shipping

Molecular Characterization

This product is supplied and shipped with blue ice, please inquire the shipping
cost.

Storage
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 100.7 kDa. The protein migrates as 126

Upon receipt, store it immediately at -20°C or lower for long term storage.

kDa±5 kDa when calibrated against Star Ribbon Pre-stained Protein Marker

Please avoid repeated freeze-thaw cycles.

under reducing (R) condition (SDS-PAGE) due to glycosylation.

This product is stable after storage at:-20°C to -70°C for 5 years in lyophilized state;

Endotoxin
Less than 10 EU/mg by the LAL method.

-70°C for 12 months under sterile conditions after reconstitution.

Protein A<5 ppm of protein tested by ELISA.

Host Cell Protein<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA<0.02 ng/μg of protein tested by qPCR.

SDS-PAGE

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9/18/2024

GMP Human VCAM-1 Protein, Fc Tag
Catalog # GMP-VC1H25

GMP Human VCAM-1 Protein, Fc Tag on SDS-PAGE under reducing (R)condition. The gel was stained with Coomassie Blue. The purity of the protein
is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA

Immobilized GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25)
at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with alinear range of 2-63 ng/mL (Routinely tested).

Bioactivity-SPR

GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) captured onProtein A Chip can bind Human ITGA4&ITGB1 Heterodimer Protein, His
Tag&Tag Free (Cat. No. IT1-H52W1) with an affinity constant between 1.00
nM - 50.0 nM as determined in a SPR assay (Biacore 8K) (QC tested).

www.acrobiosystems.com

9/18/2024

GMP Human VCAM-1 Protein, Fc Tag
Catalog # GMP-VC1H25

Bioactivity-Stability

The Cell based assay shows that GMP Human VCAM-1 Protein, Fc Tag (Cat.
No. GMP-VC1H25) is stable at 37℃ for 24 hours.

The Cell based assay shows that GMP Human VCAM-1 Protein, Fc Tag (Cat.
No. GMP-VC1H25) is stable after freezing and thawing 3 times.

The SPR based assay shows batch-to-batch consistency between Acro's GMP
and PG VCAM-1.

MANUFACTURING SPECIFICATIONSACROBiosystems GMP grade products are produced under a quality management system and in compliance with relevant guidelines: Ph. Eur General Chapter
5.2.12 Raw materials of biological origin for the production of cell-based and gene therapy medicinal products; USP<92>Growth Factors and Cytokines Used in Cell
Therapy Manufacturing; USP<1043>Ancillary Materials for Cell, Gene, and Tissue-Engineered Products; ISO/TS 20399-1:2018, Biotechnology - Ancillary
Materials Present During the Production of Cellular Therapeutic Products.

ACROBiosystems Quality Management System Contents：
Designed under ISO 9001:2015 and ISO 13485:2016, Manufactured and QC tested under a GMP compliance factory.
Animal-Free materials
Materials purchased from the approved suppliers by QA
ISO 5 clean rooms and automatic filling equipment
Qualified personnel
Quality-related documents review and approve by QA
Fully batch production and control records

www.acrobiosystems.com

9/18/2024

GMP Human VCAM-1 Protein, Fc Tag
Catalog # GMP-VC1H25

Equipment maintenance and calibration
Validation of analytical procedures
Stability studies conducted
Comprehensive regulatory support files
Request For Regulatory Support Files（RSF）

ACROBiosystems provide rigorous quality control tests (fully validated equipment, processes and test methods) on our GMP grade products to ensure that they meetstringent standards in terms of purity, safety, activity and inter-batch stability, and each bulk QC lot mainly contains the following specific information:
SDS-PAGE
Protein content
Endotoxin level
Residual Host Cell DNA content
Residual Host Cell Protein content
Biological activity analysis
Microbial testing
Mycoplasma testingIn vitro virus assay
Residual moisture
Batch-to-batch consistency

Background
Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin
superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, aswell as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears tofunction in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signaltransduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.

Clinical and Translational Updates

www.acrobiosystems.com

9/18/2024