Application Note

Channel:Cells:
Tools:

INa, IK, ICa
human iPS 		
cardiomyocytesPatchliner®

Voltage and current clamp recordings ofCellartis® hiPS-CM on Nanion’s Patchliner®
The electrophysiology team at Nanion Technologies GmbH, Munich.
Cells kindly provided by Takara Bio Europe AB.

SummaryResults

Human induced pluripotent stem (iPS) cell-derived car-Figure 1 shows recordings in the current and voltage

diomyocytes have the potential to provide the ultimate

clamp mode of Cellartis® hiPS-CM. Current traces showmodel system for identifying potential anti-arrythmic ef-

exemplary NaV and CaV currents. In the same cell, re-fects of drugs during routine safety screening. Takara Bio

corded in the current clamp mode, action potentials

Europe AB is providing a human iPS cell-derived cardio-(AP) could be elicited. Figure 2 shows raw data traces

myocyte product line for use in testing the efficacy andand current-voltage relations of Na+ and K+ currents assafety of pharmaceutical therapies. The ability to char-recorded in the voltage clamp mode.

acterize the ion channel profile of these cells and recordaction potentials at a reasonable throughput is essential
to fully realise the potential of this kind of product line.

AB

Building on the success of recording stem cells on thePatchliner®1-3, Cellartis® hiPS-CM, human iPS cell-derived
cardiomyocytes, have now been characterized on thePatchliner® in the voltage and current clamp mode.

Pulse protocol

In this Application Note we present data using an 8-channel Patchliner®. In the voltage clamp mode, voltage-dependent Na (NaV), K (KV) and Ca++2+CD(Cav) channel cur-rents were recorded.
As expected, action potentials could be elicited in thecurrent clamp mode. Furthermore, spontaneous action
potentials could be recorded as well.
Seal Resistance

Cslow

Rs

1,1 ± 0,19 GW

18,6 ± 2 pF

7,8 ± 3 mV

Table 1:
Sucess rate of Cellartis® hiPS-CM (Takara Bio Europe AB) as recorded on an
8-channel Patchliner equipped with temperature control.

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Figure 1:
Currents recorded from Cellartis® hiPS-CM (Takara Bio Europe AB). A Elicited
AP as recorded in the current-clamp mode. B NaV and CaV current in thevoltage clamp mode. The inset shows the applied voltage pulse protocol.
C Zoom-in to Ca currents, corresponding current-voltage relation (D).

Application NoteA

Figure 3 shows action potentials from Cellartis® hiPS-CM

B

recorded in the current clamp mode. Here, the APs wereeither induced (arrow at x-axis represents timepoint ofstimulus) or occured spontaneously.
In conclusion, human induced pluripotent stem (iPS)
cell-derived cardiomyocytes from Takara Bio Europe AB
exhibit an ion channel profile (NaV, KV, CaV) as expected
in human cardiomyocytes. These ion channels expressedin conjunction contribute to the action potentials which
are elicited in these cells.

Figure 2:
A Current-voltage relation of Na channel mediated currents (circles) and K
outward current (triangles). B Raw data traces as recorded in the voltage
clamp mode showing Na+ and K+ currents.

The data provide evidence that the Patchliner® is well
suited to recording stem cell-derived cardiomyocytes ina higher throughput environment given its flexibility andreliability.
of TTX.

Figure 3:
Induced and spontaneous action potentials recorded from Cellartis® hiPS-CM (Takara Bio Europe AB) in the current clamp mode. Arrow in the x-Axis
represents timepoint of applied stimulus.

References
Electrophysiology

1. Becker et al., 2013, Jpharmtox, JPM-06117
2. Stoelzle et al., 2011. JBS. 16 (8): 910–916

Whole-cell recordings were conducted according to

3. Stoelzle at al., 2011. Front Pharmacol. 2 (76) doi: 10.3389/

Nanion’s standard procedure for the Patchliner®. Current-fphar.2011.00076

voltage recordings were made using voltage steps from-60 mV to 50 mV for 20 ms increasing in 10 mV steps, fromMethods

a holding potential of -120 mV (NaV); -40 mV to 50 mV for
200 ms increasing in 10 mV steps from a holding potential

Cellsof -100 mV (CaV), a 50 ms pre-pulse to -40 mV inactivatedhuman iPS cell-derived cardiomyocytes (Cellartis® hiPS-Na currents. APs were elicited using a 1 ms current pulseCM) were supplied by Takara Bio Europe AB.

(calculated for each cell individually).

Cell culture
Cells were cultured and harvested according toNanion’s standard cell culture protocol for stem cell-derived cardiomyocytes.

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