MABSOL® Biotinylated Proteins
Bring pre-labeled biotinylated proteins directly to your benchwww.acrobiosystems.com

PD-1
TNF-alpha

HER2
CD3E

FcRn
BCMA

CD16a

ContentI.

Introduction

II. Application
III. Product Series
IV. Key Features

V. Case Studies
VI. PrecisionAvi Series Product List
VII. UltraLys Series Product List

VIII. Testimonials
CD19
EGFR
ROR1
OX40

PD-L1

TIGIT

CTLA-4

Introduction
Biotin is commonly used as a protein tag to facilitate the detection, purification, and immobilization of theproteins.
The bond between biotin and its binding partner Avidin (or Streptavidin)
is unique in the following ways:- Strong (Ka of 1015 M-1)
- Specific- Multi-moiety- Stable- Minimal interferenceWith characteristics mentioned above, biotin-avidin (streptavidin) system is now considered a versatile
independent technology in following applications.

Application
ELISA
Biotinylated proteins can be used in ELISA as two-way antibodies for both capture and detection with highspecificity and detection sensitivity.

FACS
Biotinylated proteins can be used along fluorophore-tagged SA to detect/isolate cells expressing particularsurface markers.

Biopanning
It is a technique often used for the selection of phage displays during antibody drug development.
Biotinylated proteins can be used with SA-coated magnetic beads/surface in biopanning with higher coating
density and uniformed antigen presentation.

SPR
It is a standard method used by pharmaceutical researchers to study protein binding kinetics. Biotinylated
proteins can be used along with Biacore Sensor Chip SA for SPR analysis with low baseline drift and noise,and low activity loss during surface regeneration cycle.

Immuno-capture and Enrichment
Biotinylated proteins can be used to isolate antibodies from plasma or other biological fluid for subsequent
analyses with high sensitivity, and the processed sample can be easily analyzed in quantitative mass
spectrometry.

Product Series
MABSOL® biotinylated protein collection includes two unique and complimentary product series, thePrecisionAvi series built upon the AviTagTM technology, and the UltraLys series produced using the in-housedeveloped chemical labeling method. These products are made with every attention to details.

PrecisionAvi Series
An exclusive collection of ready-to-use AviTagTM biotinylated proteins
The products in this series are exclusively produced using the AviTagTM technology. Briefly, a unique 15 aminoacid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction.
The single lysine residue in the Avi tag is enzymatically biotinylated by the E. coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many
advantages for commonly used binding assays. The biotinylation
happens on the lysine residue of Avi tag, and therefore does notinterfere with the target protein’s natural binding activities. In
addition, when immobilized on an avidin-coated surface, theprotein orientation is uniform because the position of the Avi tagin the protein is precisely controlled since biotin is labeled at single
lysine in the Avi tag.

UltraLys Series
A unique series of chemically labeled biotinylated proteins with ultra-sensitivity
The products in this series are produced using our in-house developed chemical labeling approach. The
primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated withbiotins.

Chemical labeling usually results in multiple biotin attachment on asingle protein molecule, which could potentially leads to higher
detection sensitivity.

Key Features
High Quality Authentic Human Proteins
High Bioactivity & Detection Sensitivity
Low Batch-to-Batch Variation

Case Studies
SPR: Antibody Optimization with Biotinylated FcRn
The half-life and efficacy of a therapeutic antibody largely
depends on its Fc fragment. To obtain candidate antibody
with desired pharmaceutical properties, the interaction
between Fc fragment and Fc receptors needs to beevaluated. SPR is a common assay used for determing
binding affinity between Fc and Fc receptors.
Efficient coating and chip regeneration present a major
challenge in this approach. As shown here, the combo of
ACROBiosystems’ AviTagTM biotin-labeled FcRn and GE’s
sensor Chip-SA delivers satisfactory result in a binding
assay against Herceptin.

Fig. 1 Biotinylated Human FcRn / FCGRT & B2M, Avi Tag(AviTag™) (Cat. No. FCM-H82W4) coupled to SA coatedsensor chip can bind Herceptin with an affinity constant of
2.24 μM as determined in a SPR assay (Biacore 2000). The
data is provided by Biaffin GmbH &Co KG, Germany.

Immuno-capture with Biotinylated TNF-alphaTNF-alpha antibody is widely used in treating autoimmune diseases. In order to optimize the administration,it’s very important to monitor the serum concentration of TNF-alpha antibody. Our collaborator AbSciex has
shown that, using ACROBiosystems’s biotinylated TNF-alpha (Cat. No. TNA-H821R), TNF-alpha antibody in theserum could be easily detected by immune-capture coupled with quantitative MS/MS.
Biotinylated TNF-alpha is first coupled tostreptavidin coated magnetic beads, and thencapture TNF-alpha antibody in the serum. After
that, MS/MS is applied to quantify TNF-alphaantibody. Immuno-capture with biotinylated
TNF-alpha significantly increases the sensitivity
by ten folds.

Fig. 2 Chromatograms spiked Adalimumab in human plasma: blank plasma,
5, 50, and 500 ng/ml

Inhibitor Screening with Biotinylated PD-1
ACROBiosystems has developed a PD1-PD-L1 inhibitor screening assay kit (Cat. No. EP-101) for rapid and highthroughput screening of candidate inhibitory antibodies or small molecules of the PD1 pathway.
This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new PD-1
pathway inhibitors. The assay takes advantage of our in-house developed binding of biotinylated human PD-1
to immobilized human PD-L1 in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA
platform. Briefly, we provide you with a human PD-1-Biotin protein, a human PD-L1 protein, an anti-PD-1
neutralizing antibody (as method verified Std.), and streptavidin-HRP reagent. Both biotinylated PD-1 and PDL1 proteins are expressed in the HEK293 cells.

Fig. 3 Immobilized human PD-L1 protein at 2 μg/mL (100μL/well) can bind biotinylated human PD-1 with a linear
range of 0.038 - 0.6 μg/mL when detected by StreptavidinHRP.

Fig. 4 Inhibition of PD-1-PD-L1 binding by Anti-PD-1
neutralizing antibody is measured using the PD-1
[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay
Pair (Cat. No. EP-101).

PrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

2B4

2B4-H82F0SpeciesStructureSize

2B4 (22-221)

FcAvi25ug, 200ug

4-1BB (24-186)

FcAvi

25ug, 200ug

4-1BB (24-186)

Avi

His

25ug, 200ug

B7-1 (35-242)

Fc

Avi

25ug, 200ug

B7-1 (35-242)

Avi

His

25ug, 200ug

B7-2(26-247)

Avi

His

25ug, 200ug

B7-2 (26-247)

Fc

Avi

25ug, 200ug

B7-H4(29-258)

Avi

His

25ug, 200ug

B7-H5 (33-194)

Avi

His

25ug, 200ug

BAFF (134-285)

25ug, 200ug

Human
4-1BB

41B-H82F7
Human

4-1BB

41B-H82E3
Human

B7-1

B71-H82F2
Human

B7-1

B71-H82E9
Human

B7-2

CD6-H82E2
Human

B7-2

CD6-H82F5
Human

B7-H4

B74-H82E2
Human

B7-H5

B75-H82E1
Human

BAFF

BAF-H82F3
Human

Avi

Fc

PrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

BAFF

BAF-H82Q2

Species

Structure

Size

Avi

BAFF (134-285)

25ug, 200ug

BAFFR (10-71)

Fc

Avi

25ug, 200ug

BCMA(1-54)

Fc

Avi

25ug, 200ug

BCMA(1-53)

Fc

Avi

25ug, 200ug

BTLA (31-134)

Fc

Avi

25ug, 200ug

CD155 (21-343)

Fc

Avi

25ug, 200ug

CD27 (21-192)

Fc

Avi

25ug, 200ug

CD28 (19-152)

Fc

Avi

25ug, 200ug

CD30 (19-379)

Avi

His25ug, 200ug

CD38 (43-300)

Avi

His

25ug, 200ug

CD40 (21-193)

Avi

His

25ug, 200ug

CD40 (21-193)

Fc

Avi

CD47 (19-139)

Fc

Avi

25ug, 200ug

CD47(19-139)

His

Avi

25ug, 200ug

His
Human

BAFFR

BAR-M82F0
Mouse

BCMA

BC7-H82F0
Human

BCMA

BCA-C82F4
Cynomolgus

BTLA

BTA-H82F8
Human

CD155

CD5-H82F6
Human

CD27

TN7-H82F6
Human

CD28

CD8-H82F2
Human

CD30

CD0-H82E6
Human

CD38

CD8-H82E7
Human

CD40

CD0-H82E8
Human

CD40

CD0-H82F2

His

25ug, 200ug

Human
CD47

CD7-H82F6
Human

CD47

CD7-H82E9Human

CD52

CD52 (25-36)

CD2-H82F3
Human

Fc

Avi

25ug, 200ug

PrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

CTLA-4CT4-H82F3Species

StructureSize

CTLA-4 (37-162)

Fc

Avi

25ug, 200ug

CTLA-4 (37-160)

Avi

His

25ug, 200ug

DNAM-1 (19-247)

Fc

Avi

25ug, 200ug

Human
CTLA-4CT4-C82E5
Cynomolgus

DNAM-1DN1-H82F9
Human

EGFR (vIII)

EGR-H82E0

EGFR(25-29)

EGFR (298-645)

Avi

His

25ug, 200ug

Human
EMMPRIN

CD7-H82E0

EMMPRIN (22-205)

Avi

His

25ug, 200ug

EpCAM (24-265)

Avi

His

25ug, 200ug

CD64 (25-297)

Avi

His

25ug, 200ug

Avi

His

25ug, 200ug

CD32a (35-217)

Avi

His

25ug, 200ug

CD32b (46-217)

Avi

His

25ug, 200ug

Avi

His

25ug, 200ug

Avi

His

Human
EpCAM

EPM-H82E8
Human

Fc gamma RI / CD64

CD4-M52E8
Mouse

H167

Fc gamma RIIA /CD32a

CDA-H82E6

Fc gamma RIIA /CD32a

CDA-H82E7

Fc gamma RIIB /CD32b

CDB-H82E0

Fc gamma RIIIA /CD16a

CDA-H82E8

Fc gamma RIIIA /CD16a

CDA-H82E9

Fc gamma RIIIB /CD16bCDB-H82E1

Fc gamma RIIIB /CD16b

CDB-H82E4

FcRn

FCM-H82W4

CD32a (36-218)
Human
R167

Human

Human
F176

CD16a (17-208)
Human
V176

Human

Human

CD16a (17-208)

25ug, 200ug

CD16b (NA2) (17-200)

Avi

His

25ug, 200ug

CD16b (NA1) (17-200)

His

Avi

25ug, 200ug

Human
FCGRT (24-297)

Avi

B2M (21-119)

Strep II

His
25ug, 200ug

HumanPrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

FcRn

FCM-M82W6

FcRn

Species

Mouse

FOLR1

Size

FCGRT (22-297)

Avi

B2M (21-119)

Twin-StrepFCGRT (23-298)

Avi

His
25ug, 200ug

His

FCM-R82W7

25ug, 200ug
Rat

FcRn

Structure

FCM-C82W5

Cynomolgus /Rhesus

B2M (21-119)

Strep IIFCGRT (24-297)

Avi25ug, 200ug
B2M (21-119)

Strep IIFOLR1 (25-232)

FO1-M82E9

His

Avi

His

25ug, 200ug

Mouse
G-CSF R

G-CSF R (25-621)

GCR-H82E4

Avi

His

Hemagglutinin (HA) (19-523)

Avi

His

25ug, 200ug

Hemagglutinin (HA) (19-338)

Avi

His

25ug, 200ug

Hemagglutinin (HA) (17-339)

Avi

His

25ug, 200ug

HGF R (25-932)

Avi

His

HVEM (39-202)

Fc

Avi

25ug, 200ug

Human
Hemagglutinin (HA)

HA1-V82E1
Influenza

Hemagglutinin (HA)

HA1-V82E2
Influenza

Hemagglutinin (HA)

HA1-V82E4
Influenza

HGF R

MET-H82E125ug, 200ug

Human
HVEM

HV4-H82F125ug, 200ug

Human
IGF-II

Avi

IG2-H82F9

Fc

IGF-II (25-91)

25ug, 200ug

Human
IgG Fc

IG1-H82E2

Human IgG1 Fc (100-330)

Avi

Mouse IgG2a Fc (98-330)

Avi

25ug, 500ug

IL-17 RA (33-320)

Fc

Avi

25ug, 200ug

IL-17 RA (33-320)

Avi

His

25ug, 200ug

His

25ug, 500ug

Human
IgG FcIGA-M8210
Mouse

IL-17 RAILA-H82F1
Human

IL-17 RAILR-H82E0
Human

IL-21

IL1-H82F7
Human

IL-21 (23-155)

Fc

Avi

25ug, 200ug

PrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

IL23A & IL12B

ILB-H82W6

Species

Structure
His

IL23A (20-189)
25ug, 200ug

Human
IL-4

Avi

Size

IL4-H82E0

IL12B (23-328)
IL-4 (25-153)

Avi

25ug, 200ug

His

Human
IL-4 R alpha

ILR-H82E9

IL-4 R alpha (26-232)

Avi

His

25ug, 200ug

IL-6 R alpha (20-365)

Avi

His

25ug, 200ug

IL-7 R alpha (21-236)

Fc

Avi

25ug, 200ug

LILRB1 (24-458)

Fc

Avi

25ug, 200ug

LILRB2 (22-461)

Fc

Avi

25ug, 200ug

LILRB3 (24-443)

Fc

Avi

25ug, 200ug

LILRB5 (24-458)

Fc

Avi

25ug, 200ug

M-CSF R (20-512)

Avi

His

25ug, 200ug

Mesothelin (296-580)

His

Avi

25ug, 200ug

Nectin-2 (32-360)

Fc

Avi

25ug, 200ug

Nectin-3 (58-400)

Fc

Avi

25ug, 200ug

NS1 (796-1148)

Avi

His

25ug, 200ug

OX40 (29-216)

FcAvi25ug, 200ug

Human
IL-6 R alphaCD6-H82E8
Human

IL-7 R alphaIL7-H82F8
Human

LILRB1

CDJ-H82F7
Human

LILRB2LI2-H82F5
Human

LILRB3

CDA-H82F6
Human

LILRB5

CDC-H82F8
Human

M-CSF R

CSR-H82E0
Human

Mesothelin

MSN-H82E9
Human

Nectin-2

CD2-H82F8
Human

Nectin-3

PV3-H82F3
Human

NS1

NS1-Z82E9
Zika virus

OX40

OX0-H82F7
HumanPrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

OX40

TN4-H82E4

Species

Structure

Size

OX40 (29-216)

Avi

His25ug, 200ug

PCSK9 (31-692)

Avi

His

25ug, 200ug

PCSK9 (31-692)

Avi

His

25ug, 200ug

PCSK9 (35-694)

Avi

His

25ug, 200ug

Human
PCSK9

PC9-H82E7
Human

D374Y

PCSK9

PCY-H82E7
Human

PCSK9

PC9-M82E1
Mouse

PD-1

PD1-H82E4

PD-1 (25-167)

Avi

His

PD-1 (25-167)

Fc

Avi

PD-1 (25-167)

Fc

Avi

PD-L1 (19-238)

Fc

Avi

PD-L1 (19-238)

Avi

His

25ug, 200ug

PD-L1 (19-238)

Fc

Avi

25ug, 200ug

PD-L2 (20-219)

Avi

His

25ug, 200ug

ROR1 (30-403)

Fc

ROR1 (30-403)

Avi

SCF (26-190)

Avi

His25ug, 200ug

CD33 (18-259)

Avi

His

25ug, 200ug

25ug, 200ug

Human
PD-1

PD1-H82F2

His25ug, 200ug

Human
PD-1

PD1-M82F425ug, 200ug

Mouse
PD-L1

PD1-H82F3
Human

PD-L1

PD1-H82E5

His25ug, 200ug

Human
PD-L1

PD1-M82F5
Mouse

PD-L2

PD2-H82E8
Human

ROR1

RO1-H82F4
Human

ROR1

RO1-H821y

Avi

25ug, 200ug

25ug, 200ug

Human
SCF

SCF-H82E1
Human

Siglec-3

CD3-H82E7
HumanPrecisionAvi Series (AviTagTM) Product List
MoleculeCat. No.

SIRP alpha

CDA-H82F2

SpeciesStructure

Size
Avi

Fc

SIRP alpha (31-370)

25ug, 200ug

Human
TIGIT

TIT-H82F1

TIGIT (22-141)

Fc

Avi

25ug, 200ug

TIGIT (22-141)

Avi

His

25ug, 200ug

Human
TIGIT

TIT-H82E5
Human

TNF-alpha(HPLC-verified)

TNA-H82E3

TNF-alpha(HPLC-verified)

TNA-M82E9

TRAIL R2

TR2-H82E6

TNF-alpha (77-233)

His

Avi

25ug, 200ug

TNF-alpha (80-235)

His

Avi

25ug, 200ug

Human

Mouse
DR5 (56-182)

Avi

VSIG8 (22-263)

Fc

VEGF R2 (20-764)

Avi

His

25ug, 200ug

Human
VSIG8

VS8-H82F2

Avi

25ug, 200ugHis

25ug, 200ug

Human
VEGF R2

KDR-H82E5
Human

VEGF121

VE1-H82E7

Avi

His

VEGF121 (27-147)

25ug, 200ug

His

Avi

VEGF164 (27-190)

25ug, 200ug

His

Avi

VEGF165 (27-191)

25ug, 200ug

Human
VEGF164

VE4-M82Q3
Mouse

VEGF165

VE5-H82Q0
HumanUltraLys Series (Chemical labeling) Product List
MoleculeCat. No.

B7-H4

B74-H8222

Species

StructureSize

B7- H4 (29-258)

His

25ug, 200ug

CD19 (20-291)

Fc

25ug, 200ug

Human
CD19

CD9-H8259

Human

CD3E & CD3D

Fc

His

CD3D (22-105)

Fc

Flag

25ug, 200ug
Human

CD3 epsilon

CD3E (23-126)

CDD-H82W0

CDE-H8223

CD3 epsilon (23-126)

His

25ug, 200ug

CX3CL1 (25-341)

His

25ug, 200ug

EphB4 (16-539)

His

25ug, 200ug

EpCAM (24-265)

His

25ug, 200ug

EpCAM (24-265)Fc

25ug, 200ug

ErbB3 (20-643)

His

25ug, 200ug

Human
CX3CL1CX1-H8221
Human

EphB4

EP4-H8229
Human

EpCAM

EPM-H8223
Human

EpCAM

EPM-H8254
Human

ErbB3

ER3-H8223
Human

FcRn

His

B2M (21-119)

Strep II25ug, 200ug
Human

FGF basic

FCGRT (24-297)

FCM-H8286

BFF-H8117

50ug, 500ug

FGF basic (143-288)

Human
GrowthHormone R

GHR-H8222

GM-CSF

GMF-H8214

Growth Hormone R (27-264)

His

50ug

Human
25ug, 200ug

GM-CSF (18-144)

Human
GPA33

GPA33 (22-235)

GP3-H8224
Human

His

25ug, 200ug

UltraLys Series (Chemical labeling) Product List
MoleculeCat. No.

Her2

HE2-H822R

Species

Structure

Size

Her2 (23-652)

His

25ug, 200ug

Human
IL-6

IL-6 (30-212)

25ug, 200ug

IL-1 alpha (113-271)

25ug, 200ug

IL6-H8218
Human

IL-1 alphaILA-H8213
HumanMesothelin

Mesothelin (296-580)

MSN-H8223

His

25ug, 200ug

Human
Mesothelin

Fc

MSN-H826x

Mesothelin (296-580)

25ug, 200ug

Human
PD-1

PD-1 (25-167)

PD1-M8259

Fc

25ug, 200ug

Mouse
Protein L

RPL-P814R

SCF

SCF-M8228

N/A

N/A

SCF (26-189)

500ug, 2mg

His

25ug, 200ug

SOST (24-213)

25ug, 200ug

Mouse
SOST

His

SOT-H8245
Human

TFPI

TFPI (29-282)

TFI-H8226

His

25ug, 200ug

HumanTransferrin R

His

TFR-H8243

Transferrin R (89-760)

25ug, 200ug

Human
TNF-alphaTNA-H8211

TNF-alpha (77-233)

25ug, 200ug

TNF-alpha (77-233)

25ug, 200ug

VEGF165 (27-191)

25ug, 200ug

Human
TNF-alphaTNA-H821R
Human

VEGF165

VE5-H8210

Human

Testimonials“I have been very satisfied with ACROBiosystems’ products and customer service. Their technical support
team has been extraordinarily proactive in making sure that our needs are being fully addressed. They always
work closely with us to help us find the products that suited our research the best. This includes offering anarray of samples for free for our preliminary testing. The demonstration of such awareness of subtle issues
surrounding our research was quite unexpected and highly impressive.
Vidal F.de la Cruz, Founder & Principal of Fomento Pharma, LLC“Rubicon Biotechnology considers ACROBiosystems a preferred vendor to supply us with high quality proteinsfor our drug development programs, including using their proteins in our in vivo studies. We have contractedwith ACROBiosystems to manufacture high purity custom proteins and they proposed, and met, aggressive
delivery times that allowed Rubicon to start some critical proof-of-concept studies. Acro’s customer service
was extremely knowledgeable and responsive and kept us up to date on their progress. We never felt out ofthe loop. We are extremely satisfied with ACROBiosystems and would recommend them to anyone in theprotein discovery and development fields.
Richard Richieri, Founding Partner & Chief Operating Officer, Rubicon Biotechnology“TNF-alpha immunocapture is easy to automate and offers a very specific sample preparation by isolating only
the compound of interest. Furthermore the high specificity generates very low and robust MS/MS signal tonoise ratios and has allowed us to gain a factor of ten in sensitivity. Thus our mass spectrometer which
hitherto was not sensitive enough, can now detect levels of concentration of our therapeutic monoclonal
antibody which are compatible with the clinical diagnosis.
Jean-François Jourdil, University Hospital of Grenoble“In my ten years of research experience, I have found that the protein products produced by ACROBiosystems
are equal to none. They are exceptional in their purity and activity and, as such, have greatly enhanced thequality of my research. It has been a pleasure to find a great multitude of proteins, most with a variety of tags,
all offered by a single company. In addition, the efficiency and cordiality of their customer service matches theexcellence of their products, resulting in one very satisfied customer!
Adam D. Friedman, University of North Carolina at Chapel HillTNF-alpha
FcRn
HER2
CD3E

CD16a

BCMA

PD-1

+ 1 800-810-0816 (US / Canada)+ 86 400-682-2521 (Asia & Pacific)
techsupport@acrobiosystems.com
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