ABBOMAX, INC
Custom Services
Polyclonal antibody production
Monoclonal antibody production
Phosphospecific antibody production
Antibody purification and labeling
Custom assay development
Recombinant protein expression
Peptide synthesis and conjugation
Custom antibody screening & mapping
Kinetic data
Custom Synthesis Services
Custom Liposome Preparation
Order information:
Order may be place by phone, fax, email or website.
Custom service is available 8:00AM to 4:00PM (Pacific Time), Monday-Friday.

AbboMax, Inc
2528 Qume Drive, Suite 8
San Jose, California, 95131. USA
Tel: 408-573-1898
Fax: 408-573-1858
Email: info@abbomax.com
Website: www.abbomax.com

CONTETNTS
Custom Antibody Services:
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AbMAX Polyclonal peptide-antibody package
AbMAX Polyclonal protein-antibody production
AbMAX Monoclonal antibody package
AbMAX Peptide carrier protein conjugation
AbMAX Polyclonal phosphospecific/paired antibody package
AbMAX Monoclonal phosphospecific/paired antibody package
AbMAX Antibody purification
 AbMAX Epitope-affinity purification
 AbMAX Protein A purification
 AbMAX MAP peptide purification
 AbMAX Antibody Fab Fragmentation
 AbMAX Antibody F(ab)2 Fragmentation
 AbMAX Large-scale antibody production in vivo
 AbMAX Large-scale antibody production in vitro

60001
60002
60003
60004
60008
60009
60005
60005PC
60005PA
60005MA
60011ab1
60012ab2
60013
60014

Custom Assay Development Services: AsyMAX Immunoassay development
 AsyMAX Bioassay development
 AsyMAX Kinase assay development

60021
60022
60023

Custom recombinant protein service:RecMAX Molecular cloning and screening
RecMAX Transfection and cell line selection
RecMAX Recombinant protein expression & purification
RecMAX Large-scale protein purification

60031
60032
60033
60034

Custom peptide synthesis
 PepMAX Peptide synthesis

70001

Custom Antibody screening & Mapping

80001

Kinetic Data

80002

Shipping:
All shipping and handling charges are prepaid by AbboMax and added to the invoice along with the
shipment. If you would like to use your own carrier, please supply the account number when the order isplaced. Orders are taken before 12:00PM (Pacific Time) can be shipped same day for the next day
delivery domestically. All international orders will be shipped international priority using FedEx.

Product Warranty:
AbboMax Inc. warrants its services and products to original purchaser against defects in material andworkmanship under normal use and application for a period of one year from data of purchase. AbboMax
sole obligation under this warranty shall be to replace defective products that do not perform to our statedspecifications. In no case shall AbboMax be liable for incidental or consequential damages. This warranty
applies only to products in the original container that have been handled and stored in accordance with
AbboMax recommendations. This warranty is in lieu of all other warranties, express or implied, including
warranties of merchantability and fitness for a particular purpose.

2|PageCustom Antibody Services
AbMax polyclonal peptide-antibody package (Cat# 60001)
Antigenic Index:

Window = 7

1.0
0.8
0.5
0.2
0.0
-0.2
-0.5
-0.8
-1.0

AbboMax provides regular polyclonal
antibody package, the host can be any of
the following animals at your choice –rabbits, chicken, mice and other animals.
This package includes the following items

80
90
100
110
120
130
VR W N P N E KF I V E S RE N P A I N SS K L A P HP R L K V S KN T K L T LS P K L Y L CH D K H S EL C H N K T QQ F R Q R I

Kyte/Doolittle hydrophilicity:

Window = 7

4.0
3.0
2.0
1.0
0.0
-1.0
-2.0
-3.0
-4.0
80
90
100
110
120
130
VR W N P N E KF I V E S RE N P A I N SS K L A P HP R L K V S KN T K L T LS P K L Y L CH D K H S EL C H N K T QQ F R Q R I

Surface Probability:

Window = 7

1.0
0.8

 Consultation of epitope selection in terms ofantigenicity index, hydrophilicity and secondary
structure 10-19 mg of peptide (80% checked by HPLC, up to
15 mer); BSA/KLH conjugation
 2 rabbits (or other animals) with AbboMax
immunization protocol
 Pre-immune serum (2-5 ml/rabbit); 4 s.c.
immunizations; 2 bleeds with total 100 ml ofserum
 ELISA titration for all bleeds, Western Blot (Optional):
Dot Blot (Optional)

0.6
0.4
0.2
0.0
80
90
100
110
120
130
VR W N P N E KF I V E S RE N P A I N SS K L A P HP R L K V S KN T K L T LS P K L Y L CH D K H S EL C H N K T QQ F R Q R I

Chou Fasman 2º structure:
Helix

Window = 7
Sheet

Turn

1.5
1.2
1.0
0.8
0.5
0.2
0.0
80
90
100
110
120
130
VR W N P N E KF I V E S RE N P A I N SS K L A P HP R L K V S KN T K L T LS P K L Y L CH D K H S EL C H N K T QQ F R Q R I

Data will be provided with the project: Peptide: HPLC and Mass Spec
 Conjugation datasheet
 ELISA titration for all serum samples

AbMax polyclonal protein-antibody Package (Cat# 60002)*Customers will provide immunogen(s) for immunization
The package includes the following items:
 2 rabbits with AbboMax immunization protocol
 Pre-immune serum (2-5 ml/rabbit); Antiserum 100 ml
 ELISA titration for all bleeds
 Western blot (Optional); Dot blot (Optional)

AbMax monoclonal antibody package (Cat# 60003)
Deliver in 3 months; Confidentiality is assured by AbboMax, Inc.
The package includes the following items:
 5 mice with AbboMax standard immunization protocol,
 Phase 1—Pre-immune serum and antiserum (1 ml @ 1:100 dilution) with ELISA titration.
 Phase 2—Cell fusion and parental clone screening Phase 3—Subcloning and cryogenic vialing Phase 4—Large-scale monoclonal antibody production for a selected monoclone Phase 5 (Optional)—Protein A purification according to the monoclone isotype
Data will be provided with the project: Peptide: HPLC and Mass Spec if peptide is required KLH/BSA Conjugation datasheet
 ELISA titration for antisera, ELISA screening for all the clones
 Isotyping for the monoclones

3|PageAbMax peptide carrier protein conjugation (Cat# 60004)
The peptide sequence information should be available for protein chemistry determination.
Data will be provided with the project: KLH/BSA/GGG/THY/PPD (a selected carrier protein) conjugation datasheet
 Conjugation efficiency determination (optional)—the molar ratio of peptide/carrier protein, SDS-PAGE
verification

AbMax polyclonal phosphospecific/paired antibody package (Cat# 60008)
 Consultation ofphosphospecific site
prediction and epitope
selection
 10-20 mg of phosphospecificand non-phospho peptides(80% checked by HPLC, up
to 15 mer)
 BSA/KLH conjugation
 2 rabbits with AbboMax
immunization protocol forsite-modification antigen

 Pre-immune serum (2-5ml/rabbit); 4 s.c.
immunizations; 2 bleeds withtotal 100 ml of serum
 ELISA analysis for all bleeds
and antibodies
 Affinity purification forphosphospecific and paired
antibody production
 Dot blot to confirm thepurified antibodies

AbMax monoclonal phosphospecific/paired antibody package (Cat# 60009)
Deliver in 3.5 months; Confidentiality is assured by AbboMax, Inc.
Phase 1—Project initiation, mice immunization and titration
 Consultation of epitope selection and modification
 Phosphospecific peptide and non-phosphospecific peptide
synthesis, purification, conjugation
 5 mice with AbboMax standard immunization protocol
 Pre-immune serum and antiserum (1 ml @ 1:100 dilution) with
ELISA titration
Phase 2—Fusion and parental clone screening Three population of parental clones will be selected according to
ELISA Phosphospecific clones will be guaranteed among these three
population clones
Phase 3—Subcloning Limit dilution on parental clones confirmed by customer’s
selection
 Isotyping for all the subclones
Phase 4—Large-scale antibody production for a specificmonoclone 50 ml ascites, ELISA confirmation, protein concentration andisotypingPhase 5 (Optional)—Protein A purification according to themonoclone isotype

4|PageAbMax antibody affinity purification (Cat# 60005)
We provide the following purification according to customer requirements
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Peptide epitope affinity purification
Protein A/G affinity purification
MAP system affinity purification
All the purified antibody will be verified by antigen (protein/peptide) specific ELISA
Antibody enrichment and recovery efficiency

SDS-PAGE

Protein Spectrum

Dot Blot

ELISA Titration
SampsleO.D

Purified Antibodies

Prebleed

Test Bleed

AP Antibody

AP Antibody

Running ThroughProtein A

Mix of rabbit sera

Mix of Antisera

F1

F2

Waste

Purified Antibody

Dilution Test 1 Test 2 Test 1 Test 2 Test 1 Test 2 Test 1 Test 2 Test 1 Test 2
1:1K

0.309

0.312

F1

F2

0.659

0.667

1.114

1.124

1:5K

0.168

1:25k

0.061

0.169

0.11

0.107

0.613

0.617

0.066

0.084

0.077

0.177

1:125K

0.167

0.062

0.062

0.063

0.068

0.065

0.063

0.104

0.061

0.064

0.074

0.065

0.066

0.06

0.063

0.061

0.06

0.063

0.066

0.063

0.062

0.064

0.068

0.067

0.062

0.061

0.064

0.064

0.064

0.062

0.065

0.069

0.063

1.596

1.609

1.908

1.919

1.08

1.075

0.166

0.963

0.962

1.228

1.198

0.165

0.06

0.274

0.277

0.687

0.685

0.069

0.06

0.06

0.062

0.063

0.302

0.307

1:625K

0.06

0.058

0.059

0.059

0.106

1:3125K

0.057

0.06

0.059

0.06

0.061

1:15625K 0.058

0.06

0.061

0.058

0.062

0.067

1XPBS

0.059

0.059

0.058

0.064

0.065

0.056

AbMax Antibody Fab fragmentation (Cat# 60011ab1)
AbMax Antibody F(ab)2 fragmentation (Cat# 60012ab2)
To void antigen capping and internalization, antibody
fragmentation is usually employed. In the antibody proteolytic
fragmentation service, we provide the following items:
 The outline of antibody fragmentation process Antibody concentration and purity
 Results confirmed on electrophoresis.

5|PageAbMax Large-scale antibody production in vivo (Cat# 60013)
AbMax Large-scale antibody production in vitro (Cat# 60014)
Monoclonal antibodies can be produced in vitro or in vivo. AbboMax provides small or large
scaled antibody production at your request.
 Cell line maintenance and expanding Antibody collection and treatment
 Protein concentration and ELISA validation.

Custom Assay Development Service
AsyMax Immunoassay development (Cat# 60021)
AsyMax Bioassay development (Cat# 60022)
AsyMax Kinase activity assay development (Cat# 60023)
The scientists with AbboMax have been involving in immunology and cell biology research andproduct development for more than 15 years. With the state-of-art technology, we can help you to
co-develop immunoassays, bioassays related to immunology, kinase activity assay with
Phosphorylated protein measurement for your specific needs.
 Consultation of assay format selection
 Co-develop assays and finalize the parameters.
 Protocol writing, material and assay transferring.

Custom Recombinant Protein Service
RecMAX Molecular cloning and screening
RecMAX Transfection and cell line selection
RecMAX Recombinant protein expression & purification
RecMAX Large-scale protein purification

60031
60032
60033
60034

Molecular cloning and protein over-expression is an effective tool in preparing protein-antigens
when the information of a cloned gene is available. The other advantage of using the recombinant
protein is to recognize an effective affinity epitope involved in drug discovery. Dedicatedscientists at AbboMax are willing to assist you to achieve your goal.
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Primer design and expression system selection
Molecular cloning and cell line selection
Mini-prep of selected clones
Large-scale protein over-expression
Protein purity and concentration checking, SDS-PAGE confirmation
Western blot (optional)

6|PageCustom Peptide Synthesis (Cat#70001)
Peptide synthesis—AbboMax offers high quality, fast-turn-around custom peptide synthesis:
Fmoc solid phase peptide synthesis, purified
by reversed-phase HPLC
Overnight synthesis of 10 mer or less highquality crude peptides
Fastest turn-around-time (TAT) in theindustry
Friendly and professional customer support

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D-amino acid synthesis, MAP synthesis
Cyclization—disulfide bond 1, 2, and 3;
amide cyclization
Phosphorylation, methylation, acetylating
Peptide fluorescence/dye labeling: biotin
FITC/5-FAM, MCA, EDANS/DABCYL.

Free consultation services---We also offer free consultation services of epitope selection andphosphosphorylated Serine/Threonine, Tyrosin prediction.

Custom Antibody Screening & Mapping Services (Cat#80001)
HIGHLIGHTS






Simple step – simply add theinterested antibody sample into theloading well of pre-coated chips
Fast – within 2 minutes, the curve ofaddition different antibodies appear
indicating antibody pairs.
Sensitive – detect antibodies in T. C
super or ascites samples at 0.1-1µg/100 µl.

Specific – assay does not crossreact with any other non-specificproteins
Reliable – results are similar tostandard ELISA-based assays
Convenient-You only need to add
samples onto the chip-wells.



Mapping antibody pairs may be a tedious labor work when quantitative sandwich ELISA is needed. In
addition to our Services and Products listed above, AbboMax has the capability to support you directlywith label-free analyses* of your proteins.

Antibody screening and epitope mapping:
Screening : Anti TNF antibody binding to TNFLigands
Binding is specifichuman TNFglutathione S-transferase (GST)

Anti TNF antibody binding to TNF

2.5

Anti TNF antibody AS1-P
Mouse IgG1

3.0

Anti TNF antibody AS1-P (mouse IgG1)
Mouse IgG1

2.0

Two monoclonal antibodies AS1-P & AS3-P
bind to different epitopes

Ligand: human TNFAnti TNF antibody AS1-P
Anti TNF antibody AS3-P
Mouse IgG1

2.5
2.0

[nm]

[nm]

1.5

1.5

We canscreen yourto determine their binding characteristics and accelerate your
Bufferantibodies
wash
1.0
1.0
development efforts. Just speak to us (@408-321-9898)
about your specific needs and we will
0.5
Buffer wash
0.5
be happy to provide a quote on timing
andcosts.
Anti TNF antibody
Anti GST antibody

0.0
0

1000

2000

3000

4000

0.0
0

400

800

1200

1600

Time [s]* using LamdaGen Corporation’s LightPath System ™ (www.lamdgen.com)

7|PageKinetic Data Services (Cat# 80002)
Looking for kinetic data? We can provide kONS, kOFFS and kDS as per the following:
L ig a n d im m o b iliz e d o n th e s u rfa c e

C A 2 a n a ly te in je c te d in to th e L IP s ys te mCBS

2 .8  M

k O B S = (1 1 0 .7 1 ± 1 .9 1 ) * 1 0 -3 s -1

2 .0

(8 8 .4 2 ± 1 .5 4 ) * 1 0 -3 s -1
(2 3 .3 8 ± 2 .5 1 ) * 1 0 -3 s -1

8 .5  M

k O F F = (3 .6 2 ± 0 .0 1 ) * 1 0

-3

s -1

(3 .8 8 ± 0 .0 1 ) * 1 0

-3

s -1

1 .5

 [nm](5 7 .5 7 ± 1 .3 2 ) * 1 0 -3 s -1

6 .8  M

4 .2  M

2 .5(3 .4 4 ± 0 .0 1 ) * 1 0 -3 s -1
1 .0

(3 .5 4 ± 0 .0 1 ) * 1 0 -3 s -1

0 .5

0 .0
-1 0 0 0

0

1000

20 00

3000

4 000

5000

6 000

7000

8000

T im e [s ]F ro m k O B S , k O F F a n d th e c o n c e n tra tio n o f th e a n a lyte , C , w e c o m p u te k O N a n d K D v a lu e s :k OBS -

kON =

kO FF

k O FF

KD =

C

kON

K in e tic p a ra m e te rs d e te rm in e d fro m > 5 0 s e n s o rg ra m s fro m > 1 0 s e n s o rs a c q u ire d o n 4 d iffe re n t a n d in d e p e n d e n t m a ch in e sy ie ld a ve ra g e va lu e s w ith s ta n d a rd d e via tio n s o f:

k O F F = ( 3 .6 3 ± 0 .3 ) * 1 0 -3 s -1
k O N = ( 1 1 .0 2 ± 3 .1 ) * 1 0 -3 s -1  M -1
KD

= ( 0 .3 3 ± 0 .1 1 )  M

Quantitation is also possible as provided in the following example:
Quantitation : streptavidin / anti-streptavidin
Ligand : streptavidin
Assay : increasing amount of anti-streptavidin from whole serum(exact concentration of anti-streptavidin not known, here it is estimated)

0.6 nM

1.2 nM

2 nM

5 nM

0

500

1000

1500

10 nM 40 nM“Saturation level” as a function of protein concentration
A * [C]Fit model :sat“one site binding”
kD + [C]200 nM

3.0
2.5

1.5
1.0
0.5

kD = 23.76 ± 2.11 nM

0.0
2000

2500

3000

3500

1.0

Time [s]0.8Normalized  [nm] [nm]2.0

kD = 21.25 ± 3.00 nM

0.6

0.4

0.2

0.0
0

50

100

150

200

Concentration [nM]

Tel. 408-573-1898, Fax: 408-573-1858; e-mail: info@abbomax.com, www.abbomax.com
Copyright 2020 AbboMax Inc. All rights reservedCustom Synthesis Services (Cat#80003)
AbboMax provides chemistry support to biotech and pharmaceutical companies. Our expertise in the areaof organic/heterocyclic/combinational and medical chemistry enable us to assist your research in a fastdelivery and cost-effective manner. Please contact us if you haveany needs in bulk chemical compound synthesis.
Here are examples of our custom services:
Reference and standard compounds
Precursors and intermediates
Primary and focused libraries
Simple and complex peptides
Process development
N(CH2CO2K)2
OCH2CH2O

N(CH2CO2K)2
N(CH2CO2K)2
OCH2CH2O

N(CH2CO2K)2

Me
Me
NH

CO2K

CO2K

N(CH2CO2Me)2
OCH2CH2O

N(CH2CO2Me)2

CHO

Me

Custom Liposome Preparation (#80004)
AbboMax offers custom liposome preparation as well as a various preformed liposome reagents with alarge number of common lipid compositions to support the needs of research organizations involved inliposome drug delivery. Our high quality liposomes collection and custom services are performed byexperienced formulators using the highest quality lipids only from premier suppliers. Our products areeasy to use to save you time, cost and improve the productivity and reliability of your research. You cantrust our products for batch-to-batch consistency and reproducibility. All products are sterile filtered andproperly packaged in vials to ensure long-term stability when stored refrigerated.
Plain Non-PEG Liposomes
Plain PEGylated Liposomes
Liposomes with Ammonium Sulfate
Transfection Reagents: Cationic Liposomes
Please contact us for further information:
info@abbomax.com
www.abbomax.com

Tel. 408-573-1898, Fax: 408-573-1858; e-mail: info@abbomax.com, www.abbomax.com
Copyright 2020 AbboMax Inc. All rights reserved

ABBOMAX INC Custom Peptide/Antibody Order Form
Please fax, email or call AbboMax to place orders
Amino Acid Chart
A(Ala)

AlanineC(Cys) CysteineH(His)

Histidine

M(Met)

Methionine

T(Thr) Threonine

R(Arg)

Arginine

Q(Gln) Glutamine

I(Ile)

Isoleucine

F(Phe)

Phenylalanine W(Trp) Tryptophan

N(Asn)

Asparagine

E(Glu) Glutamic Acid

L(Leu)

LeucineP(Pro)

ProlineY(Tyr) Tyrosine

D(Asp)

Aspartic Acid

G(Gly) Glycine

K(Lys)

LysineS(Ser)

SerineV(Val) Valine

Date: ________________________________

PO#: _____________________________

Phone: ___________________________

Fax: ____________________________

Shipping Address:

Billing Address:

Attn Name:________________________

Attn Name:_______________________

Institute/Company:____________________

Institute/Company:__________________

Address:_______________________________

Address: ___________________________________________________________________________________________________________________________________________________________________________

PeptideNameSequences

Length ofaa

PurityQuantity

Modification

Antibody Production
Polyclone Monoclone

AbboMax, Inc
2528 Qume Drive, Suite: #8
San Jose, CA 95131
Tel: 408-573-1898
1-866-628-9898 (USA)Fax: 408-573-1858
info@abbomax.com

Tel. 408-573-1898, Fax: 408-573-1858; e-mail: info@abbomax.com, www.abbomax.com
Copyright 2020 AbboMax Inc. All rights reserved