Laboratory
Filtration
Products

Table of Contents

03

Table of Contents
Filtration and ultrafiltration are essential process steps in nearly all environmental,
chemistry and bioscientific laboratory applications.
Sartorius supplies a wide range of individual filter papers, microporous membranes, filtration devices, ultrafiltration units and protein purification devices tosuit these applications. This catalog provides a condensed overview of theSartorius Lab Filtration product range. Please contact us directly for specialty
catalogs – available for in-depth ­technical information.

Ultrafiltration
Protein Concentration
DNA Concentration
Protein Purification
Virus Purification and Concentration

10
50
57
59

Filtration Devices
Minisart® Standard Syringe Filters
Medical Device CE-Minisart® Syringe Filters
Claristep® Syringeless Filtration 
NEW! Sartolab® P20 Pressure Filter Units 
Sartolab® RF 50
NEW! Sartolab® RF | BT
Sartolab® Multistation
Sartoclear Dynamics® Lab P15
NEW! Sartoclear Dynamics® Lab V
Chemical Compatibility

66
76
83
89
91
93
96
98
100
104

Basic Filtration
Filter Papers
Glass and Quartz Microfiber Filters
Membrane Filters
Blotting | Chromatography Papers & Blotting Membranes
Filtration Equipment
Combisart® Manifolds

110
115
120
129
131
141

05

Ultrafiltration
Table of Contents

Membrane Selection Guide8

Protein Concentration
Vivaspin® 500Vivaspin® 2
Centrisart® 1
Vivaspin® Turbo 4 PESVivaspin® 6
Vivaspin® 15R
Vivaspin® Turbo 15 PESNEW! Vivaspin® Turbo 15 RC
Vivaspin® 20
Vivaclear Centrifugal Filters
Vivacell 100Vivaflow® 50
Vivaflow® 50RVivaflow® 200Vivapore® Solvent Absorption Concentrators
Ultrafiltration Membrane Filters

10
12
15
17
19
21
23
25
27
30
31
35
38
41
44
46

DNA Concentration
Vivacon® 500Vivacon® 2

50
53

Protein Purification
Vivapure® Ion Exchange Protein Purification Products

57

Virus Purification and Concentration
Vivapure® Virus Purification and Concentration Kits
Adenovirus Purification with AdenoPACK Kits
Lentivirus Purification with LentiSELECT Kit

59
60
62

06

Ultrafiltration

Introduction

Introduction
Ultrafiltration is a convective process using anisotropic
semi-permeable membranes to separate macromolecularspecies and solvents – primarily on the basis of size.
By allowing solvents and salts to pass the ultrafiltration
membrane, macromolecules which are hindered to passit, are concentrated. Ultrafiltration can also be applied insolvent exchange applications. Multiple concentration andrefilling steps will progressively lead to a buffer exchange,
replacing lengthy techniques like dialysis. Althoughultrafiltration is not a standard method for separating andfractionating macromolecules, it can be used as such if themacromolecules differ at least 10 times in size. Ultrafiltration
is a gentle, non-denaturing method that is more efficientand flexible than other processes.
Ultrafiltration Methods
Sartorius offers you a comprehensive range of ultra­filtration
process methods for the concentration of your biologicalsamples.
	Centrifugal Concentration (0.1 to 100 mL starting volume)
Pressure Ultrafiltration (5 to 100 mL starting volume)	Crossflow (Tangential Flow) (0.1 to 5 L starting
volumes)
Static Absorption (3 to 20 mL starting volume)-------

Further information about the operational methods canbe found on page 8.
Typical Applications for Ultrafiltration
	Concentration | desalting of proteins, enzymes, DNA,
monoclonal antibodies, immunoglobulins, viruses andnanoparticles
	Bence Jones Protein concentration from urine samples
prior to capillary electrophoresis
	Forensic DNA sample concentration prior to sequencing
reaction	Peptide fractionation in FASP (filter-aided sample preparation)
	Free drug | hormone assays	Removal of primers from PCR amplified DNA
	Removal of labeled amino acids and nucleotides
	Deproteinization of samples
	General purpose laboratory concentration and desaltingof proteins, enzymes, DNA, biomolecules, viruses, antibodies and immunoglobulins---

Membrane Performance Characteristics
Sartorius offers an extended range of membranes to cover
the great majority of ultrafiltration requirements.
	Polyethersulfone (PES)	Cellulose Triacetate (CTA)	Hydrosart®

Further information about the properties of the different
membrane types can be found on page 9.
Process Optimization
When the highest recoveries are crucial, particularly
with solute quantities in the microgram range, Sartorius
recommends considering the following tips for optimal
ultrafiltration results:	Select the lowest MWCO membrane that suits
your application. For the highest recovery, choose amembrane MWCO which is at least half of the molecularweight of the solute to be retained.
	Avoid over-concentration. The smaller the final
concentrate volume, the more difficult it is to achievecomplete recovery. If feasible, rinse the device with one ormore drops of buffer after the first concentration cycle andthen recover it again.
	Pretreat the device overnight in distilled water with apassivation solution such as 5 % SDS, Tween 20 or
Triton X-100. Rinse thoroughly before use.

-

Solute concentration for diagnostics
Ultrafiltration devices can be used in the clinical setting
for the concentration and separation of disease markers,
such as Bence Jones protein from urine for the diagnosis ofacute multiple myeloma, and others from clinical samples,such as blood, serum, urine and cerebrospinal samples.
Use of devices for these applications require dedicated invitro Diagnostic (IVD) registered devices. IVD devices are
only available in registered countries, according to country
specific regulations. Please contact Sartorius for more
information on registered countries and availability.

08

Ultrafiltration

Membrane Selection Guide

Membrane Selection Guide
Ultrafiltration Methods
Sartorius offers you a comprehensive range of ultra­filtration process methods
for the concentration of your biological samples. The guide below will help you
select the most suitable device according to sample volume and available equipment, as well as your desired filtration speed and process control.

Centrifugal Concentration (0.1 to 100 mL Starting
Volume)Driven by the centrifugal force, ultrafiltration can be used
to purify and concentrate proteins, nucleic acids, viruses,
macromolecules and nanoparticles. This gentle process
is quick to set up and offers fast filtration speeds for mostsolutions. Sartorius offers seven Vivaspin® devices and theVivacell® 100 and Centrisart® 1 for protein concentration,
as well as Vivacon® devices for DNA and peptide
concentration | fractionation.
Pressure Ultrafiltration (5 to 100 mL Starting Volume)
Pressurized air or inert gas provide the filtration vector
for ultrafiltration. To speed up the filtration process, thepressurized Vivacell® 100 or Vivaspin® 20 can be placedon an orbital laboratory shaker. Agitation helps to prevent
membrane blocking and ensures high filtration speed.
Vivaspin® 20 and Vivacell® 100 can be run with gas pressure.
The Vivaspin® 20 can also be run by pressure-fugation, a
unique Sartorius method combining gas and pressure withcentrifugation. This is the fastest of all methods, providing
process times that are typically 30 to 50 % faster thancentrifugation.
Crossflow (Tangential Flow) (0.1 to 5 L Starting Volumes)
The sample is pumped across an ultra­filtration membrane
and then returned to the original reservoir, with pressurebuilt up in the device by a flow restrictor. The solution isprogressively concentrated as solvent and micromolecules
pass through the membrane into a separate filtrate vessel.
Reusable Vivaflow® 50R and Vivaflow® 200, as well as
disposable Vivaflow® 50, are offered for your dedicatedlaboratory crossflow filtration.
Static Absorption (3 to 20 mL Starting Volume)
This technique uses an absorbent cellulose pad mounted
behind the ultrafiltration membrane to draw solvents andmicro­molecules through the membrane. The retained
macromolecules thus concentrate at the bottom of thesample container. No additional equipment is needed.
These devices are ideal for clinical applications like urine
concentration prior to further analysis. Both Vivapore® 5
and Vivapore® 20 offer this procedure.

Ultrafiltration

Membrane Selection Guide

09

Membrane Performance Characteristics
Sartorius offers an extended range of membranes to cover
the great majority of ultra­filtration requirements. To selectthe most appropriate membranes for your application
please refer to the following guide. Please note howeverthat membrane behavior and ultimate performance, largely
depends on the specific characteristics of the solution
being processed. Where available, alternative membranes
should be tested to optimize the process performance.

Hydrosart®
Concentration | desalting of column eluates, Hydrosart®
membrane evaluation prior to upscaling. Hydrosart®
demonstrates the same properties as regenerated
cellulose, but with the added benefit of enhanced
performance characteristics and extremely low protein
binding, making it the membrane of choice for applications
such as concentration and desalting of immunoglobulin
fractions.

Polyethersulfone (PES)
Concentration | desalting of column eluates, cell culture
supernatants, etc. This is a general purpose membranethat provides excellent performance with most solutions
when retentate recovery is of primary importance. PES
membranes are usually preferred for their low foulingcharacteristics, exceptional flux and broad pH compatibility.

Regenerated Cellulose (RC)This is a hydrophillic membrane suitable for general
samples, wiith ultra-low protein adsorption and highchemical compatability. The membrane is especially well
suited to oligonucleotides and peptides. This Sartorius
RC membrane has been developed uniquely for the lab
ultrafiltration devices and applications, ensuring
optimal performance.

Cellulose Triacetate (CTA)
Free | bound drug studies and whenever the filtrate is
being analyzed. High hydrophilicity and very low nonspecific binding characterize this membrane. Cast without
any membrane support that could trap or bind passing
micro solutes, these membranes are preferred for sample
cleaning and protein removal, and when high recovery fromthe filtrate solution is of primary importance.

Membrane Selection Guide
The molecular weight cut-off (MWCO) is the molecularweight of molecules (e.g. globular proteins) which areretained by the membrane to an extent of 90 %. Therefore,
to ensure the highest recovery, select a membrane with a
MWCO that represents up to half the molecular weight ofthe solute to be retained.
Sartorius Vivaspin® ultrafiltration units are designed toconcentrate protein solutions. Therefore, the membranes
in the devices are tested for the retention of proteins andnot the passage of proteins into the filtrate vessel. PES andHydrosart® membranes have support structures, whichmight lead to some loss of protein after passage throughthe membrane.

Recommended MWCO (Da):Application
Bacteria
DNA fragments
Enzymes
Extracellular Vesicles
Growth factors
mAB
Nucleic acids
Oligonucleotides
Peptides
Virus
Yeast

< 5,000--

10,000--30,00050,000

--

--

-

-

----

100,000-> 300,000-

10Ultrafiltration

Protein Concentration

Vivaspin® 500
100 to 500 µL Samples
Vivaspin® 500 centrifugal filter units offer a simple, one-step procedure forsample preparation. They can effectively be used in fixed-angle rotors accepting
2.2 mL ­centrifuge tubes.
The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high-speed­concentrations – even with particle-loaded solutions.

Specifications
Vivaspin® 500

Please use a fixed angle rotor for
2 mL reaction vials.

Concentrator capacity

Swing bucket rotor
Fixed angle rotor

do not use
500 µL

Dimensions

Total length
Width
Active membrane area
Hold-up volume,
membrane and support
Dead-stop volume

50 mm
11 mm
0.5 cm2

Body
Filtrate vessel
Concentrator cap
Membrane

Polycarbonate
Polypropylene
Polycarbonate
Polyethersulfone

Materials of construction< 5 µL
5 µL

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]

50

11

Rotor

Fixed angle

Centrifugal force

12,000 g

Start volume

500 μLMin.

Rec.

Aprotinin 0.25 mg/mL (6,500 MW)
3,000 MWCO PES

30

96 %

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
10,000 MWCO PES
30,000 MWCO PES

15
5
5

96 %
96 %
96 %

IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES
50,000 MWCO PES
100,000 MWCO PES

10
10
10

96 %
96 %
96 %

Ultrafiltration

Protein Concentration

11

Ordering Information
Vivaspin® 500 Polyethersulfone

Qty./Pkg.

Prod. No.

3,000 MWCO

25

VS01913,000 MWCO

100

VS01925,000 MWCO

25

VS01115,000 MWCO

100

VS0112

10,000 MWCO25

VS0101

10,000 MWCO

100

VS010230,000 MWCO

25

VS0121

30,000 MWCO

100

VS012250,000 MWCO25

VS013150,000 MWCO

100

VS0132100,000 MWCO

25

VS0141

100,000 MWCO

100

VS0142300,000 MWCO

25

VS0151300,000 MWCO

100

VS0152

1,000,000 MWCO

25

VS01611,000,000 MWCO

100

VS01620.2 µm25

VS0171

0.2 µm100

VS0172-----Visit us at www.sartorius.com/Vivaspin500 to get additional info.
Find instructions on how to use Vivaspin® 500 for	Desalting and buffer exchange	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses	Urine protein concentration
	Concentration to a predefined volume
Concentration of diluted samples with increased recovery

12Ultrafiltration

Protein Concentration

Vivaspin® 2
0.4 to 2 mL Samples
The Vivaspin® 2 bridges the gap between the 500 µL and 4 mL centrifugal­concentrators. This device combines the speed of the classic Vivaspin® productswith low internal surface and membrane area for superior recoveries from very
dilute solutions.
Available with a choice of PES, Cellulose Triacetate or Hydrosart® membranes,
Vivaspin® 2 offers the highest flexibility for process optimization.
Also unique to Vivaspin® 2 is the choice of directly pipetting the concentratefrom the dead-stop pocket built into the bottom of the concentrator, or
alternatively reverse spinning into the concentrator cap. Both methods result innear total ­concentrate recoveries.

Specifications
Vivaspin® 2

10,000
MWCO

Concentrator capacity

Swing bucket rotor
Fixed angle rotor

3 mL
2 mL

Dimensions

Total length
Width
Active membrane area
Hold-up volume
of membrane
Dead-stop volume

126 mm
17 mm
1.2 cm2< 10 µL
8 µL

Body
Filtrate vessel
Concentrator cap
Membrane

Polycarbonate
Polycarbonate
Polycarbonate
PES, CTA, HY

2.0
1.5
1.0

126

0.5

0.2

Materials of construction

0.1
20 µl

Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]

17
Rotor

Fixed angle

Swing bucket

Centrifugal force

8,000 g4,000 gStart volume

2 mL
Min.

Rec.

Aprotinin 0.25 mg/mL (6,500 MW)
3,000 MWCO PES

50

96 %

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
5,000 MWCO CTA
5,000 MWCO Hydrosart®
10,000 MWCO PES
10,000 MWCO CTA
10,000 MWCO Hydrosart®
20,000 MWCO CTA
30,000 MWCO PES
30,000 MWCO Hydrosart®

12
50
22
8
10
12
5
8
5

98 %
98 %
98 %
96 %
98 %
97 %
97 %
95 %

Ultrafiltration

Protein Concentration

13

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]Rotor

Fixed angle

Centrifugal force

5,000 gStart volume

2 mL
Min.

Rec.

6
10
10
8

97 %
96 %
96 %
95 %

Vivaspin® 2 Polyethersulfone

Qty./Pkg.

Prod. No.

3,000 MWCO25

VS02913,000 MWCO

100

VS02925,000 MWCO25

VS02115,000 MWCO

100

VS0212

10,000 MWCO25

VS0201

10,000 MWCO100

VS020230,000 MWCO25

VS0221

30,000 MWCO

100

VS022250,000 MWCO25

VS023150,000 MWCO100

VS0232100,000 MWCO25

VS0241

100,000 MWCO

100

VS0242300,000 MWCO25

VS0251300,000 MWCO

100

VS0252

1,000,000 MWCO25

VS02611,000,000 MWCO

100

VS02620.2 µm25

VS0271

0.2 µm100

VS0272

IgG 0.25 mg/mL (160,000 MW)
20,000 MWCO CTA
30,000 MWCO PES
50,000 MWCO PES
100,000 MWCO PES

Ordering Information

14

Ultrafiltration

Integral deadstop
avoids risk ofconcentrating
to dryness

PES, CTA, or Hydrosart®
­membranes; Filtrate container fits
standard 15 mL tube ­carriers

Direct pipette recovery or choiceof reverse spinning concentrator
into sample cap

Protein Concentration

Vivaspin® 2 Cellulose Triacetate

Qty./Pkg.

Prod. No.

10,000 MWCO

25

VS02V1

10,000 MWCO

100

VS02V220,000 MWCO

25

VS02X1

20,000 MWCO

100

VS02X2

Vivaspin® 2 Hydrosart®

Qty./Pkg.

Prod. No.

2,000 MWCO

25

VS02H91

2,000 MWCO

100

VS02H92

5,000 MWCO

25

VS02H115,000 MWCO

100

VS02H12

10,000 MWCO

25

VS02H01

10,000 MWCO

100

VS02H02

30,000 MWCO

25

VS02H2130,000 MWCO

100

VS02H22---

Ordering Tips
	Choose a membrane pore size at least 50 % smaller than the size of the molecule to be retained.
	It is usually best to select polyethersulfone membranes to achieve thefastest concentrations.
	Usually choose cellulose triacetate for protein removal or ultrafiltrate recovery.
	Usually choose Hydrosart® membranes for the highest recovery with Ig fractions.

-----

Visit us at www.sartorius.com/Vivaspin2 to get additional info.
Find instructions on how to use Vivaspin® 2 for	Desalting and buffer exchange	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses	Urine protein concentration
	Concentration of diluted samples with increased recovery	Sample preparation for radio immunoassay

Ultrafiltration

Protein Concentration

15

Centrisart® 1
Centrisart® 1* is a ready-to-use unit for small-volume, centrifugal ultrafiltration
to separate proteins from low molecular weight substances in biological samples.
Centrisart® 1 features a unique design that enables ultrafiltration in the direction
opposite to centrifugal force. This is so effective in preventing premature
blockage of the filter that even whole blood samples can be deproteinized.
The ultrafiltrate is collected in the floatering filtrate tube, where it is readily
accessible without disassembly.

----

Centrisart® 1 is ideal for the following ­applications:	Drug binding studies
	Determination of metabolites in serum	Protein removal from blood samples
	Cleaning of liposomes	Virus removal

Specifications

Direction ofultrafiltration

Centrisart® 1
Concentrator capacity

Swing bucket rotor
Fixed angle rotor

2.5 mL
2.5 mL

Dimensions

Ultrafiltrate iscollected in the floating
filtrate tuber, where it isreadily accessible
without disassembly.

Total length
Width
Active membrane area
Hold-up volume of membrane
Dead-stop volume

93 mm
14 mm
0.79 cm2< 5 µL100 µL

Materials of construction

Centrifuge tube
Floater tube
Cap
Membrane

Polystyrene
Cellulose propionate
Polyethylene
CTA, PES

Ultrafiltrate

Typical Performance Characteristics

Ultrafilter

Time to filter
50 % of sample
volumeTime to filter
90 % of sample
volumePassage oftest molecule

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO
10,000 MWCO
20,000 MWCO

300 min35 min9 min

N |A
80 min20 min0%
2%2%

IgG 0.25 mg/mL (160,000 MW)
100,000 MWCO

13 min35 min3%

IBlue Dextran 0.1 mg/mL(2,000,000 MW)
300,000 MWCO

9 min

25 min28 %Retentate

Centrifugal force

2.5 mL samples were loaded into each device. The devices were centrifuged at 2,000 g until the required filtrate volumes
had been reached.
Devices can be used in conical or flat bottom centrifuge adaptors.
* Centrisart is a registered ­trademark in the U.S. and the European Union

16

Ultrafiltration

Protein Concentration

Ordering Information
Qty./Pkg.

Prod. No.

5,000 MWCO CTA

1213229----------E

10,000 MWCO CTA

1213239----------E

20,000 MWCO CTA

1213249----------E

100,000 MWCO PES

1213269----------E*

300,000 MWCO PES

1213279----------E*IVD device article code available only in IVD registered countries, according to country specific regulations

References
P. Nebinger and M. Koel:
Determination of acyclovir by
ultrafiltration and high-performanceliquid chromatography.
J. Chromatography 619, 342-344 (1993)
F. da Fonseca-Wollheim, K.-G. Heinze,
K. Lomsky and H. Schreiner:
Serum ultrafiltration for the eliminationof endogenous interfering substancesin creatinine determination.
J. Clin. Chem. Clin. Biochem. 26,
523-525 (1988)

R. H. Christenson, S. D. Studenberg,
S. Beck-Davis and F. A. Sedor:
Digoxin-like immunoreactivity
eliminated from serum by centrifugal
ultrafiltration before fluorescencepolarization immunoassay of digoxin.
Clinical Chemistry 33, 606-608 (1987)

Visit us at wwww.sartorius.com/en/products/lab-filtration-purification/
ultrafiltration-devices to get additional info.
Find instructions on how to use Centrisart® I for the high recovery of
cationised protein.

Ultrafiltration

Protein Concentration

17

Vivaspin® Turbo 4 PES
2 to 4 mL Samples
Vivaspin® Turbo 4 PES is the newest member of the ultrafiltration family andallows the fastest sample concentration with the highest recoveries. This device
can handle up to 4 mL sample volumes in swing bucket and fixed angle rotorsthat accept 15 mL conical bottom centrifuge tubes.
The Vivaspin® Turbo 4 PES optimized design and sleek internal profile ensure
maximum process speeds all the way down to the last few microliters, resulting in
more than 100-fold concentration.
The UV joining technology ensures smooth joint transition between themembrane and the plastic housing – allowing removal of the entire concentrated
sample from the unique, pipette tip-friendly dead-stop pocket.

VIVASPIN® TURBO 4
10,000 MWCO

Specifications
Vivaspin® Turbo 4 PES
Concentrator capacity

Swing bucket rotor
Fixed angle rotor

4 mL
4 mL

Dimensions

Total length
Width
Active membrane area
Hold-up volume of membrane
Dead-stop volume
swing bucket rotor
Dead-stop volume fixed angle
rotor (25°)

122.5 mm
17 mm
3.2 cm2< 10 µL
40 µL

Body
Filtrate vessel
Concentrator cap
Membrane

Styrene butadiene copolymere
Polypropylene
Polypropylene
Polyethersulfone

122

Materials of construction

17

30 µL

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]Rotor

Swing bucket

Fixed angle (25°)

Centrifuge speed

4,000 g*7,500 g*Start volume

4 mL

4 mL

Min.

Rec.

Min.

Rec.

Cytochrome c (12,400 MW)
3,000 MWCO PES
5,000 MWCO PES

60
40

98 %
95 %

80
50

96 %
94 %

Lysozyme (14,300 MW)
3,000 MWCO PES
5,000 MWCO PES

65
50

95 %
94 %

70
60

93 %
92 %

α-Chymotrypsin (25,000 MW)
10,000 MWCO PES

10

95 %

8

95 %

BSA (66,000 MW)
10,000 MWCO PES
30,000 MWCO PES

10
8

98 %
96 %

7
6

97 %
97 %

* 3,000g for 100K MWCO devices in swing bucket centrifuge, 5,000 g for 100K devices in fixed angle centrifuge.

18

Ultrafiltration

Protein Concentration

Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]IgG (160,000 MW)
30,000 MWCO PES
50,000 MWCO PES
100,000 MWCO PES*

Min.

Rec.

Min.

Rec.

18
16
17

94 %
93 %
94 %

13
12
13

92 %
90 %
92 %

* 3,000 g swing-out | 5,000 g fixed angle

Ordering Information
Vivaspin® Turbo 4 Polyethersulfone

Qty./Pkg.

Prod. No.

3,000 MWCO

25

VS04T91

3,000 MWCO

100

VS04T92

5,000 MWCO

25

VS04T115,000 MWCO

100

VS04T12

10,000 MWCO

25

VS04T01

10,000 MWCO

100

VS04T02

10,000 MWCO

25

VS04T01IVD**

10,000 MWCO

100

VS04T02IVD**

30,000 MWCO

25

VS04T2130,000 MWCO

100

VS04T2250,000 MWCO

25

VS04T3150,000 MWCO

100

VS04T32

100,000 MWCO

25

VS04T41

100,000 MWCO

100

VS04T42** IVD device article codes available only in IVD registered countries, according to country specific regulations----

Visit us at www.sartorius.com/VivaspinTurbo4 to get additional info.
Find instructions on how to use Vivaspin® Turbo 4 PES for	Desalting and buffer exchange	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses	Urine protein concentration
	Separation of proteins and metabolites for disease detection

Ultrafiltration

Protein Concentration

19

Vivaspin® 6
2 to 6 mL Samples
Vivaspin® 6 concentrators have been developed to offer increased volume
flexibility and performance.
Vivaspin® 6 can process an impressive 6 mL in either swing bucket or fixed angle
rotors accepting standard 15 mL conical bottom centrifuge tubes.
The Vivaspin® 6 features twin vertical membranes for unparalleled filtration
speeds and more than 100-fold concentration. The remaining volume is easy
to read off the printed graduations on the side of the ­concentrator and themodified dead-stop pocket further simplifies direct pipette recovery of the final
concentrate.

Specifications
Vivaspin® 6

122

Concentrator capacity

Swing bucket rotor
Fixed angle rotor

6 mL
6 mL

Dimensions

Total length
Width
Active membrane area
Hold-up volume of membrane
Dead-stop volume

122 mm
17 mm
2.5 cm2< 10 µL
30 µL

Materials of construction

Body
Filtrate vessel
Concentrator cap
Membrane

Polycarbonate
Polycarbonate
Polypropylene
Polyethersulfone

17
Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]Rotor

Swing bucket

Fixed angle [25°]Centrifuge speed

4,000 g8,000 g*Start volume

6 mL
Min.

6 mL
Rec.

Min.

Rec.

Cytochrome c 0.25 mg/mL(12,400 MW) 5,000 MWCO PES –

–

90

97 %

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
10,000 MWCO PES
30,000 MWCO PES

20
13
12

98 %
98 %
98 %

12
10
9

98 %
98 %
97 %

IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES
50,000 MWCO PES
100,000 MWCO PES

18
17
15

96 %
96 %
91 %

15
14
12

95 %
95 %
91 %

Latex beads 0.004 % in DMEM
+ 10 % FCS (0.055 µm)
300,000 MWCO PES

–

–

25

99 %

Latex beads 0.004 % in DMEM
+ 10 % FCS (0.24 µm)
1,000,000 MWCO PES

–

–

4

99 %

Yeast 1.0 mg/mL (S. Cerevisiae)
0.2 µm PES

4

97 %

3

97 %

* 6,000 g for 100K MWCO devices

20

Ultrafiltration

Protein Concentration

Ordering Information
Vivaspin® 6 Polyethersulfone

Qty./Pkg.

Prod. No.

3,000 MWCO25

VS06913,000 MWCO

100

VS06925,000 MWCO

25

VS06115,000 MWCO

100

VS0612

10,000 MWCO25

VS0601

10,000 MWCO100

VS0602

10,000 MWCO25

VS0601IVD*

10,000 MWCO100

VS0602IVD*

30,000 MWCO25

VS0621

30,000 MWCO

100

VS062250,000 MWCO25

VS063150,000 MWCO

100

VS0632100,000 MWCO25

VS0641

100,000 MWCO

100

VS0642300,000 MWCO25

VS0651300,000 MWCO

100

VS0652

1,000,000 MWCO25

VS06611,000,000 MWCO

100

VS06620.2 µm25

VS0671

0.2 µm100

VS0672*IVD device article codes available only in IVD registered countries, according to country specific regulations----

Visit us at www.sartorius.com/Vivaspin6 to get additional info.
Find instructions on how to use Vivaspin® 6 for	Desalting and buffer exchange	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses	Urine protein concentration
Concentration of diluted samples with increased recovery

Ultrafiltration

Protein Concentration

21

Vivaspin® 15R
2 to 15 mL Samples
Vivaspin® 15R is designed for initial sample volumes of 2 to 15 mL and featuresa modified ­regenerated cellulose ­membrane; H­ ydrosart®. This membrane is ideal
where extremely high recovery with very low adsorption is needed. Examples ofthese applications include desalting and ­concentration of Ig fractions.

----

Advantages
	Ultimate recovery with low adsorption (95 – 98 %)	Exceptionally fast concentration time (30 x in 15 min.)	Convenient application protocol with easy handling	Easy scale-up to 0.1 to 5 L with Vivaflow® 50R or 200 with Hydrosart®
membranes	Very low hold-up volume (< 20 µL)
15
10
5Specifications
Vivaspin® 15R

116

30

Concentrator capacity

Swing bucket rotor
Fixed angle rotor

15 mL
12.5 mL

Dimensions

Total length
Width
Active membrane area
Hold-up volume of membrane
Dead-stop volume

116 mm
30 mm
3.9 cm2< 20 µL
30 µL

Materials of construction

Body
Filtrate vessel
Concentrator cap
Membrane

Polycarbonate
Polypropylene
Polypropylene
Hydrosart®

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]Rotor

Swing bucket

Fixed angle [25°]Centrifuge speed

3,000 g6,000 gStart volume

15 mL

12.5 mL

Min.

Rec.

Min.

Rec.

Aprotinin 0.1 mg/mL* (6,500 MW)
5,000 MWCO
47

95 %

45

95 %

Cytochrome c 0.25 mg/mL*(12,400 MW)
5,000 MWCO
10,000 MWCO

45
25

96 %
94 %

45
18

96 %
94 %

α-Chymotrypsin 0.25 mg/mL*(25,000 MW)
5,000 MWCO
10,000 MWCO

50
25

98 %
98 %

45
18

98 %
98 %

Ovalbumin 1.0 mg/mL*(45,000 MW)
10,000 MWCO
30,000 MWCO

20
15

98 %
94 %

14
12

98 %
94 %

Spin

Recover22

Ultrafiltration

Protein Concentration

Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]
Min.

Rec.

Min.

Rec.

BSA 1.0 mg/mL* (66,000 MW)
30,000 MWCO

18

98 %

15

98 %

IgG 0.1 mg/mL* in DMEM
(160,000 MW)
30,000 MWCO

30

98 %

25

96 %

* proteins other than IgG made up in 50 mM potassium phosphate, 150 mM sodium chloride, pH 7.4

Ordering Information
Vivaspin® 15R Hydrosart®

Qty./Pkg.

Prod. No.

2,000 MWCO

12

VS15RH91

2,000 MWCO

48

VS15RH92

5,000 MWCO

12

VS15RH115,000 MWCO

48

VS15RH12

10,000 MWCO

12

VS15RH01

10,000 MWCO

48

VS15RH02

30,000 MWCO

12

VS15RH21

30,000 MWCO

48

VS15RH22----

Visit us at www.sartorius.com/Vivaspin15R to get additional info.
Find instructions on how to use Vivaspin® 15R for	Desalting and buffer exchange
Preparation of biological nanoparticles and medical nanocarriers
Concentration and purification of viruses
Urine protein concentration
Concentration of diluted samples with increased recovery

Ultrafiltration

Protein Concentration

23

Vivaspin® Turbo 15 PES
4 to 15 mL Samples
Vivaspin® Turbo 15 enables the fastest ­sample concentration with the highest
recoveries. This device can handle a sample volume of up to 110 or 15 mL in fixed
angle or swing bucket rotors that accept 50 mL conical bottom centrifuge tubes.
The optimized design and sleek internal profile of Vivaspin® Turbo 15 ensure
maximum process speeds all the way down to the last few microliters, which
results in more than 100-fold concentration.
The UV joining technology ensures smooth joint transition between themembrane and the plastic housing – allowing removal of the entire sample
concentrated in the unique, pipette-friendly dead-stop pocket.

Specifications
Vivaspin® Turbo 15 PES

118

Concentrator capacity

Swing bucket rotor
Fixed angle rotor (25 °)Dimensions

Total length (concentrator insert) 77 mm
Total length (in tube with cap) 118 mm
Diameter (concentrator insert) 27 mm
Active membrane area
7.2 cm2
Hold-up volume of membrane < 10 μL
Dead-stop volume forswing-bucket rotor
100 μL
Dead-stop volume for
fixed-angle rotor
60 μL

Materials of construction

Body

27

Filtrate vessel
Concentrator cap
Membrane

15 mL
9 mL

Styrene butadiene
copolymer
Polypropylene
Polypropylene
Polyethersulfone (PES)

Typical Performance Characteristics
Time to concentrate up to 20× [min.] at
20 °C and solute recovery [%]Rotor

Swing bucket

Fixed angle [25°]Centrifuge speed

4,000 g*4,000 g*Start volume

15 mL

9 mL

Min.

Rec.

Min.

Rec.

Cytochrome c* (12,400 MW)
5,000 MWCO PES

30

98 %

50

98 %

Lysozyme* (14,300 MW)
5,000 MWCO PES

33

96 %

50

96 %

α-Chymotrypsin** (25,000 MW)
10,000 MWCO PES
10

95 %

10

95 %

BSA** (66,000 MW)
10,000 MWCO PES
30,000 MWCO PES

99 %
98 %

10
10

99 %
98 %

*2,000 g for 100K MWCO devices

10
8

24

Ultrafiltration

Protein Concentration

Typical Performance Characteristics
Time to concentrate up to 20× [min.] at
20 °C and solute recovery [%]IgG** (160,000 MW)
30,000 MWCO PES

Min.

Rec.

Min.

Rec.

23

95 %

17

95 %

* 0.25 mg/mL ** 1 mg/mL

Ordering Information
Vivaspin® Turbo 15 Polyethersulfone

Qty./Pkg.

Prod. No.

3,000 MWCO

12

VS15T91

3,000 MWCO

48

VS15T92

5,000 MWCO

12

VS15T115,000 MWCO

48

VS15T12

10,000 MWCO

12

VS15T01

10,000 MWCO

48

VS15T02

10,000 MWCO

12

VS15T01IVD***

10,000 MWCO

48

VS15T02IVD***

30,000 MWCO

12

VS15T2130,000 MWCO

48

VS15T2250,000 MWCO

12

VS15T3150,000 MWCO

48

VS15T32

100,000 MWCO

12

VS15T41

100,000 MWCO

48

VS15T42*** IVD device article codes available only in IVD registered countries, according to country specific regulations------

Visit us at www.sartorius.com/VivaspinTurbo15 to get additional info.
Find instructions on how to use Vivaspin® Turbo 15 for	Desalting and buffer exchange	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses	Urine protein concentration
	Concentration of diluted samples with increased recovery
	Concentration to a predefined volume	Depyrogenation of the ultrafiltration devices
	Concentration of mammalian cell culture supernatants

Ultrafiltration

Protein Concentration

25

Vivaspin® Turbo 15 RC
4 to 15 mL Samples
Vivaspin® Turbo 15 RC allows fastest sample concentration with highest
recoveries. This device can handle up to 11 or 15 mL sample volumes in fixed
angle or swing bucket rotors accepting 50 mL centrifuge tubes.
The Vivaspin® Turbo 15 RC optimized design and sleek internal profile ensure
maximum process speeds right the way down to the last few micro litres
leading to more than 100-fold concentration. The hydrophillic regenerated
cellulose (RC) is suitable for general samples, with ultra-low protein absorbtion
and high chemical compatability. The membrane is especially well suited
to oligonucleotides and peptides and has been developed uniquely for lab
ultrafiltration applications.
The solvent free heat weld technology allows for a smooth transition between
the membrane and plastic housing, providing complete sample recovery
from the unique pipette friendly dead stop pocket. Combined with the PES
counterpart the Vivaspin® Turbo range offers the best membrane, whatever the
sample.
118

Specifications
Vivaspin® Turbo 15 PES

27

Concentrator capacity

Swing bucket rotor
Fixed angle rotor (25 °)Dimensions

Total length (concentrator insert) 77 mm
Total length (in tube with cap) 118 mm
Diameter (concentrator insert) 27 mm
Active membrane area
8.1 cm2
Hold-up volume of membrane < 10 μL
Dead-stop volume forswing-bucket rotor
100 μL
Dead-stop volume for
fixed-angle rotor
60 μL

Materials of construction

Body
Filtrate vessel
Concentrator cap
Membrane

15 mL
9 mL

Styrene butadiene
copolymer
Polypropylene
Polypropylene
Regenerated Cellulose (RC)26

Ultrafiltration

Protein Concentration

Typical Performance Characteristics
Time to concentrate up to 20× [min.] at
20 °C and solute recovery [%]Rotor

Swing bucket

Fixed angle [25°]Centrifuge speed

4,000 g***6,000 gStart volume

15 mL

11 mL

Min.

Rec.

Min.

Rec.

Cytochrome c* (12,400 MW)
5 MWCO RC

23

94 %

37

92 %

Lysozyme* (14,300 MW)
5 MWCO RC

23

94 %

37

89 %

α-Chymotrypsin** (25,000 MW)
10 MWCO RC
7

93 %

9

92 %

BSA** (66,000 MW)
10 MWCO RC**
30 MWCO RC*

8
4

94 %
96 %

10
4

98 %
93 %

Gamma Globulin (160,000 MW)
50 MWCO RC**
17
100 MWCO RC**
18

95 %
89 %

11
12

96 %
89 %

* 0.25 mg/mL ** 1 mg/mL *** 3,000 g for 100K MWCO devices

Ordering Information
Vivaspin® Turbo 15 Regenerated Cellulose

Qty./Pkg.

Prod. No.

5,000 MWCO

12

VS15TR11

5,000 MWCO

48

VS15TR12

10,000 MWCO

12

VS15TR01

10,000 MWCO

48

VS15TR02

30,000 MWCO

12

VS15TR21

30,000 MWCO

48

VS15TR22

50,000 MWCO

12

VS15TR31

50,000 MWCO

48

VS15TR32

100,000 MWCO

12

VS15TR41

100,000 MWCO

48

VS15TR42-----

Visit us at www.sartorius.com/VivaspinTurbo15 to get additional info.
Find instructions on how to use Vivaspin® Turbo 15 for	Desalting and buffer exchange	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses	Urine protein concentration
	Concentration of diluted samples with increased recovery
	Concentration to a predefined volume	Depyrogenation of the ultrafiltration devices

Ultrafiltration

Protein Concentration

27

Vivaspin® 20
5 to 20 mL Samples
Vivaspin® 20 centrifugal concentrators have been developed to offer increased
volume flexibility and performance.
Vivaspin® 20 handles up to 140 or 20 mL in fixed angle or swing bucket rotorsthat accept 50 mL c
­ onical bottom centrifuge tubes. Featuring twin verticalmembranes for unparalleled filtration speeds, the Vivaspin® 20 can achieve
more than 100-fold ­concentrations. The remaining volume is easy to read off theprintedgraduations on the side of the ­concentrator and the modified dead-stop
pocket further simplifies direct pipette recovery of the final concentrate.
More Process Flexibility
Vivaspin® 20 is available with unique ­accessories and operating methods thatare designed to provide more process flexibility and further time savings.

Air pressure controller, VCA002

Gas Pressure Filtration
When an appropriate centrifuge is unavailable or for single sample processing,Vivaspin® 20 can be filled with up to 15 mL and then pressurized for bench-top
concentration. For even faster processing, gas pressure can be combined with­cen­trifugal force. “Pressure-fugation” is ­particularly suitable for difficult or
viscous samples, such as serum, or for use of a low process temperature, which
reduces filtration speed, and generally when ­minimum process time is essential.

Specifications
Vivaspin® 20

116

Concentrator capacity

Swing bucket rotor
Fixed angle rotor
With pressure head

20 mL
14 mL
15 mL

Dimensions

Total length
Width
Active membrane area
Hold-up volume of membrane
Dead-stop volume

116 mm
125 mm with pressure head
30 mm
6.0 cm2< 20 µL
50 µL

Body
Filtrate vessel
Concentrator cap
Pressure head
Membrane

Polycarbonate
Polycarbonate
Polypropylene
Acetal | Aluminum
Polyethersulfone

Materials of construction
30

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]ModeCentrifuge

Centrifuge

Bench top

Rotor

Swing bucket

Fixed angle [25°] Pressure

Swing bucket

Centrifugal speed | pressure

4,000 g*6,000 g

4 bar

3,000 g* +4 barStart volume

20 mL

14 mL

10 mL

10 mL

* 3,000 g for 100K devices in swing bucket centrifuge, 2,000 g for pressure-fuge devices in swing bucket

Press-fuge28

Ultrafiltration

Protein Concentration

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at
20 °C and solute recovery [%]
Min.

Rec.

Min.

Rec.

Min.

Rec.

Min.

Rec.

Cytochrome c 0.25 mg/mL(12,400 MW)
3,000 MWCO PES

110

97 %

180

96 %

60

96 %

–

–

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
10,000 MWCO PES
30,000 MWCO PES

23
16
13

99 %
98 %
98 %

29
17
15

99 %
98 %
98 %

50
32
32

98 %
97 %
97 %

14
8
8

98 %
97 %
97 %

IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES
50,000 MWCO PES
100,000 MWCO PES

27
27
25

97 %
96 %
91 %

20
22
20

95 %
95 %
90 %

46
46
42

94 %
93 %
88 %

13
13
12

97 %
96 %
94 %

Latex beads 0.004 % in DMEM
+ 10 % FCS (0.055 µm)
300,000 MWCO PES

20

99 %

35

99 %

10

99 %

–

–

Latex beads 0.004 % in DMEM
+ 10 % FCS (0.24 µm)
1,000,000 MWCO PES

4

99 %

12

99 %

4

99 %

–

–

Yeast 1.0 mg/mL (S. Cerevisiae)
0.2 µm PES

15

95 %

5

95 %

20

95 %

2

95 %

Ordering Information
Vivaspin® 20 Polyethersulfone

Qty./Pkg.

Prod. No.

3,000 MWCO12

VS20913,000 MWCO48

VS20925,000 MWCO

12

VS20115,000 MWCO

48

VS2012

10,000 MWCO

12

VS2001

10,000 MWCO48

VS2002

10,000 MWCO

12

VS2001IVD*

10,000 MWCO48

VS2001IVD*

30,000 MWCO

12

VS2021

30,000 MWCO

48

VS202250,000 MWCO

12

VS203150,000 MWCO

48

VS2032100,000 MWCO

12

VS2041

100,000 MWCO

48

VS2042300,000 MWCO

12

VS2051300,000 MWCO

48

VS2052

1,000,000 MWCO

12

VS20611,000,000 MWCO

48

VS20620.2 µm12

VS2071

0.2 µm48

VS2072* IVD device article codes available only in IVD registered countries, according to country specific regulations

Ultrafiltration

Protein Concentration

29

Vivaspin® 20 Accessories

Qty./Pkg.

Prod. No.

Air pressure controller (APC)

1

VCA002

Charge valve for pressure head1

VCA005

Diafiltration cups

12

VSA005

Female coupling1

VCA010Male coupling1

VCA011

Replacement extension line
(4 mm pneumatic tubing, 3 m)

1

VCA012

Vivaspin® 20 pressure head1

VCA200eitherpressurize
up to 5 bar with
air or inert gas

spin
up to 3,000 gorthen recoverstand in rack
Using the Vivaspin® 20 pressure head-----

Visit us at www.sartorius.com/Vivaspin20 to get additional info.
Find instructions on how to use Vivaspin® 20 for
Desalting and buffer exchange
Preparation of biological nanoparticles and medical nanocarriers
Concentration and purification of viruses
Urine protein concentration
Concentration of diluted samples with increased recovery
The workflow in protein research laboratories

30

Ultrafiltration

Protein Concentration

Vivaclear Centrifugal Filters
Vivaclear centrifugal filters are disposable microfiltration devices for the fast andreliable clarification | filtration of biological samples in the range 100 to 500 µL.
They can be used in fixed angle rotors accepting 2.2 mL centrifuge tubes.

---

Product Features	High-flux polyethersulfone
membrane
0.8 µm pore size
Low hold-up volume (< 5 µL)
Fast and reproducible performance

Specifications---

Applications	Clarification of samples before
loading in Vivapure® protein purification spin columns
Removal of particles and precipitates
Filtration of plasma and serum
Filtration of cells or cell debris

Vivaclear Centrifugal Filters
Rotor

40 – 45° fixed angle rotor

Pore size

0.8 µmDimensions

Total length
Filtrate collection tube ∅Active membrane area
Hold-up volume,
membrane plus support
Maximum RCF

43 mm
11 mm
0.34 cm2

Body
Membrane
Filtrate collection tube

Polypropylene
Polyethersulfone
Polypropylene

Materials of construction< 5 µL2,000 gOrdering Information
Vivaclear Mini 0.8 µm PES

Qty./Pkg.

Prod. No.

100

VK01P042

Ultrafiltration

Protein Concentration31

Vivacell 100
20 to 100 mL Samples
Vivacell 100 is a unique and innovative concentrator for volumes from
20 to 100 mL, and utilizes pressure, centrifuge or pressure-shake to rapidly
concentrate even samples with very high particle loads.
Vivacell 100 is designed for centrifugal concentration of samples up to 100 mL,
which makes it the largest centrifugal device available. At the same time, its
design allows for a maximum centrifugal force of 2,000 g to be used for evenfaster concentration. The patented vertical membrane design ensures thehighest performance and unmatched flexibility.

---

Vivacell 100 Utilizes:
Pressure
Centrifuge
Pressure-shake
Vivacell 100, when used as a centrifugal device, fits swng bucket rotors that
accept 250 mL bottles.

Air pressure controller, VCA002

Vivacell 100 units can also be used for single or extremely sensitive samples in
the pressurized mode only and left on a bench or placed on an orbital­laboratory
shaker for faster concentration. It can also be kept in a pressurized mode in the
refrigerator. Pressurized handling is facilitated by the use of quick connectors.
In ­whichever mode Vivacell 100 is used, the vertical membrane design inhibits­membrane ­fouling, while the built in dead-stop impedes concentration to dryness and loss of sample.

Specifications
Vivacell 100

Patented vertical membrane design

Concentrator capacity

Swing bucket rotor
With pressure head

90 mL
98 mL

Dimensions

Total length

123 mm centrifugal
197 mm with pressure head
62 mm
23.5 cm2< 250 µL
350 µL

Width
Active membrane area
Hold-up volume of membrane
Dead-stop volume

Quick release connector
Santoprene seal

Operating requirements

Rotor type
Rotor cavity

Maximum speed
Maximum pressure

Swing-bucket
To fit 250 mL (62 mm)
centrifuge bottles (maximum
cavity depth 105 mm)
2,000 g5 bar (75 psi)

Body
Filtrate vessel
Concentrator cap
Pressure head seal
Pressure head
Membrane

Polycarbonate
Polycarbonate
Polypropylene
TPE-V
AcetalPolyethersulfone

Pressurehead
Concen­trator cap

Graduations
Concen­trator

Twinverticalmembranes

Filtrate container fits standard
250 mL rotors

Materials of construction

32

Ultrafiltration

Protein Concentration

Typical Performance Characteristics
Time to concentrate up to 30× [min.] at 20 °C
90 mL Start volume

Centrifuge
Pressure (4 bar)(swing bucket,
2,000 g)No ­Orbitalagitation
shake

Soluterecovery

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
10,000 MWCO PES
30,000 MWCO PES

22
16
16

75
60
60

25
20
20

96 %
96 %
94 %

IgG 0.25 mg/mL (160,000 MW)
50,000 MWCO PES
100,000 MWCO PES

20
20

70
85

30
30

94 %
90 %

Latex beads 0.004 % in DMEM
+ 10 % FCS (0.055 µm)
300,000 MWCO PES

35

–

120

99 %

Latex beads 0.004 % in DMEM
+ 10 % FCS (0.24 µm)
1,000,000 MWCO* PES

4

5

4

99 %

%

* 2,000 g in centrifuge, 2 bar (29 psi) pressure

Ordering Information
Vivacell 100 Polyethersulfone
with Polypropylene Concentrator Cap

Qty./Pkg.

Prod. No.

5,000 MWCO

2

VC10115,000 MWCO

10

VC1012

10,000 MWCO

2

VC1001

10,000 MWCO

10

VC100230,000 MWCO

2

VC1021

30,000 MWCO

10

VC102250,000 MWCO

2

VC103150,000 MWCO

10

VC1032100,000 MWCO

2

VC1041

100,000 MWCO

10

VC1042300,000 MWCO

2

VC1051300,000 MWCO

10

VC1052

1,000,000 MWCO

2

VC10611,000,000 MWCO

10

VC10620.2 µm2

VC1071

0.2 µm10

VC1072

Ultrafiltration

Protein Concentration

33

Vivacell 100 Accessories

Qty./Pkg.

Prod. No.

Air pressure controller (APC), complete withpressure gauge, regulator, over-pressure
safety valve, female connector, 1 m extension line
(4 mm pressure tubing) with male andfemale connectors and 1 m of 6 mm inlet tubing

1

VCA002

Female coupling1

VCA010Male coupling1

VCA0114 mm pressure tubing (3 m)

1

VCA012

Replacement extension line
(4 mm pneumatic tubing, 3 m)

10

VCA014

Vivacell 100 pressure head with seals (5x)1

VCA800---Centrifuge
use with polypropylene concentrator
cap in swing out rotor
	Process convenience	Low shear, non-foaming
Less visual control--

Pressure
use with pressure head VCA800
	Simplicity and the ­highest processcontrol	Ideal for refrigerated use
	Slower concentrations--

Pressure-Shakeuse with pressure head VCA800
	Speed and process control
	Ideal for single samples

Ultrafiltration

Protein Concentration

35

Vivaflow® 50
0.1 to 3 L Samples
The unique, patented Vivaflow®* 50 ­system provides ease of use, performance,­flexibility and economy that are unrivaled by any laboratory or pilot-scale
filtration system on the market.

---

Unique Features	The thin-channel, flip-flow
recirculation path provides highcrossflow velocities with minimum
pump requirements
	No need for pressure holders
	Crystal clear for simple checking of
the concentrate and ­membrane	Unique interlocking modules withseries connectors for easy scale up
	Disposable--

Unique Performance	A single 50 cm2 module will typicallyreduce 500 mL to less than 15 mL
in under 50 minutes
	Less than 10 mL minimum system
recirculation for the highest­concentration factors	Less than 500 µL non-recoverable
hold-up volume
	Near total recoveries achievable witha single 10 mL rinse

Unique “flip-flow” thin channel flow path results in high turbulence and linear
velocity for exceptional flux, even at high concentrations.
Flip-flow recirculation path

Specifications
Vivaflow® 50

Multiple cassettes
Filtrate vessel

Sample|diafiltration
reservoir (VFA006)

Dimensions

Overall L | W | H
Channel W | H
Active membrane area
Hold-up volume (module)
Minimum recirculation volume
Non-recoverable hold-up

25 | 107 | 84 mm
15 | 0.3 mm
50 cm2
1.5 mL< 10 mL< 0.5 mL

Operating conditions

Pump flow
Maximum pressure
Maximum temperature

200 to 400 mL/min
3 bar (45 psi)
60 °C

Materials of construction

Main housing
Flow channel
Membrane support
Seals and O-rings
Pressure indicator
Flow restrictor
Fittings
TubingPolycarbonate
TPX (PMP)TPX (PMP)
Silicone
Polypropylene, SS spring
Polypropylene
Nylon
PVC (medical grade)

Single cassette* Vivaflow® is a registered trademark in the U.S., Japan and the European Union.

36

Ultrafiltration

Protein Concentration

Performance Characteristics
Time to concentrate up to 20× [min.] at 3 bar inlet pressure,
20 °C
Single device
250 mLstart volume

Three devices
1L
start volume

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
10,000 MWCO PES
10,000 MWCO RC
30,000 MWCO PES
50,000 MWCO PES

34
22
38
22
20

γ Globulins 1.0 mg/mL(160,000 MW)
100,000 MWCO PES
100,000 MWCO RC
Yeast 1.0 mg/mL(S. Cerevisiae)
0.2 µm PES

Solute recovery %DirectAfter 10 mL
rinse

49
32
55
32
29

96 %
94 %
96 %
92 %
92 %

> 99 %> 99 %> 99 %
99 %
98 %

43
40

62
58

92 %
92 %

98 %
98 %

33

47

92 %

98 %

Ordering Information
Vivaflow® 50*

Qty./Pkg.

Prod. No.

3,000 MWCO PES

2

VF05P95,000 MWCO PES

2

VF05P1

10,000 MWCO PES

2

VF05P030,000 MWCO PES

2

VF05P250,000 MWCO PES

2

VF05P3100,000 MWCO PES

2

VF05P41,000,000 MWCO PES

2

VF05P60.2 µm PES

2

VF05P7100,000 MWCO RC

2

VF05C4* Vivaflow® 50 cassettes are supplied with feed, retentate and filtrate tubing, 0.6 mm flow restrictor and luer fittings.

Vivaflow® 50 Complete Set of Accessories
Pump (230 V), Easy Load pump head (size 16),
tubing, 500 mL sample | diafiltration reservoir,cassette stand, pressure indicator, T-connectors,
series interconnectors

1

VFS502

Pump (115 V), Easy Load pump head (size 16),
tubing, 500 mL sample | diafiltration reservoir,cassette stand, pressure indicator, T-connectors,
series interconnectors

1

VFS504

Ultrafiltration

Protein Concentration

37

Accessories

Qty./Pkg.

Prod. No.

Masterflex economy drive variable speed
peristaltic pump (230 V)

1

VFA004Masterflex economy drive variable speed
peristaltic pump (115 V)

1

VFA009500 mL sample and | or diafiltration reservoir

1

VFA006Masterflex Easy-Load pump head (size 16)

1

VFA031

Vivaflow 50 stand

1

VFA032Pressure indicator (1 - 3 bar)

1

VFA034

PVC Tubing and Fittings

Qty./Pkg.

Size 16 PVC pump tubing and Luer fittings(3 m, 3.2 × 1.6 mm)

1

VFA004

Flow restrictor set, 2 each of 0.4, 0.6 and 0.8 mm

6VFA009T-connectors for running 2 stacks

2

VFA030

Series interconnectors

6

VFA031

Female luer fittings

10

VFA032

VF50 tubing kit (2 × 1 m size 16 PVC tubing withluer fittings, 2 × 50 cm size 16 PVC tubing with
0.6 mm flow restrictors, 1 x series interconnector)

1

VFA034

Flow restrictor, 0.6 mm

6VFA035®

---

Visit us at www.sartorius.com/Vivaflow50 to get additional info.
Here you can find instructions on how to use Vivaflow® 50 for
Measurement of soluble trace metals in seawaterPreparation of biological nanoparticles and medical nanocarriers
Concentration and purification of viruses

38

Ultrafiltration

Protein Concentration

Vivaflow® 50R
0.1 to 1 L Samples
Concentrate 100 mL to under 20 mL in just a few minutes or concentrate one
liter 50-fold in less than 60 minutes. Alternatively, speed up your process byusing two Vivaflow® 50R units in parallel and ­concentrate 1 L in under 30 min.
Vivaflow® 50R is a plug-and-play laboratory crossflow cassette for concentrating
up to 1 L aqueous samples. The active membrane area per device is 50 cm2.
One unit comes with all the necessary accessories for running the device with alaboratory pump and a size 16 pump head. For speeding up concentration, two­cassettes can be run simultaneously.

Return----	Fast and easy protein sample concentration
	Reusable	Concentrates volumes from 0.1 L to 1 L	Optimal for concentration of culture supernatants and viruses
	The most compact crossflow cassette with a premium Hydrosart® membrane

Sample |
Feed

Pump

Filtrate

Specifications

Vivaflow® 50R – Single cassetteVivaflow® 50R
ReturnSample |
Feed

Pump

Dimensions

Overall L | W | H
Channel W | H
Active membrane area
Hold-up volume (module)
Min. recirculation volume
Non-recoverable hold-up

24 | 100 | 100 mm
7.5 | 0.4 mm
50 cm2
1.7 mL10 mL< 0.5 mL

Operating conditions

Pump flow
Maximum pressure
Maximum temperature

200 to 400 mL/min
4 bar (60 psi)
60 °C

Materials of construction

Main housing
Flow channel
Membrane support
Seals and O-rings
Pressure indicator
Flow restrictor
Fittings
TubingAcrylic
Acrylic
Polypropylene
Silicone
Polypropylene, SS spring
Polypropylene
Nylon
PVC (medical grade)

Filtrate

Vivaflow® 50R – Two cassettes

Ultrafiltration

Protein Concentration

39

Performance Characteristics
Time to concentrate up to 20× [min.] at 3.0 bar inlet | 2.5 baroutlet pressure, 20 °C
Start volume
250 mL

Average flux
[mL/min]Directrecovery [%]Recovery after
25 mL rinse [%]Lysozyme 0.25 mg/mL(14,000 MW)
5,000 MWCO Hydrosart®
10,000 MWCO Hydrosart®

70
23

3.4
10.3

96 %
94 %

98 %
96 %

BSA 1.0 mg/mL (66,000 MW)
10,000 MWCO Hydrosart®
30,000 MWCO Hydrosart®

24
15

9.9
15.8

98 %
97 %

> 99 %> 99 %

γ Globulins 1.0 mg/mL(150,000 MW)
100,000 MWCO Hydrosart®

46

5.2

97 %

> 99 %

Performance Characteristics
Time to concentrate up to 20× [min.] at 3.0 bar inlet | 2.5 baroutlet pressure, 20 °C
Start volume
250 mL

Average flux
mL/minRecovery %Direct25 mL rinse

Start volume 1 L(one Vivaflow® 50R at 3 bar)
10,000 MWCO Hydrosart®
BSA 1.0 mg/mL

95

10.0

98 %

> 99 %Start volume 1 L(two Vivaflow® 50R
in parallel at 3 bar)
10,000 MWCO Hydrosart®
BSA 1.0 mg/mL

48

19.8

98 %

> 99 %

40

Ultrafiltration

Protein Concentration

Ordering Information
Vivaflow® 50R*
5,000 MWCO Hydrosart®

10,000 MWCO Hydrosart

Qty./Pkg.

Prod. No.

1

VF05H11

VF05H0®

1

VF05H2100,000 MWCO Hydrosart

1

VF05H4®

30,000 MWCO Hydrosart®

* Vivaflow® 50R and filtrate tubing, 0.6 mm flow restrictor, luer fittings and a pressure indicator.

Vivaflow® 50R Complete Set of Accessories
Pump (230 V), Easy-Load pump head (size 16), tubing,
500 mL sample | diafiltration reservoir

1

VFS202

Pump (115 V), Easy-Load pump head (size 16), tubing,
500 mL sample | diafiltration reservoir

1

VFS204Masterflex economy drive variable speed peristaltic pump (230 V) 1

VFP001Masterflex economy drive variable speed peristaltic pump (115 V)

1

VFP002500 mL sample and | or diafiltration reservoir

1

VFA006Masterflex Easy-Load pump head (size 16)1

VFA012Size 16 tubing and Luer fittings (3 m, 3.2 × 1.6 mm)

1

VFA004

T-connectors for running 2 cassettes

2

VFA030Flow restrictor set, 2 each of 0.4, 0.6 and 0.8 mm

6VFA009Female luer fittings, size 16

10

VFA032

Flow restrictors, 0.6 mm

6VFA035

Accessories

Tubing and Fittings--

Visit us at www.sartorius.com/Vivaflow50R to get additional info.
Here you can find instructions on how to use Vivaflow® 50R for	Preparation of biological nanoparticles and medical nanocarriers	Concentration and purification of viruses

Ultrafiltration

Protein Concentration

41

Vivaflow® 200
0.5 to 5 L Samples
Concentrate 250 mL to under 20 mL in just a few minutes or concentrate one
liter 50-fold in less than 30 minutes. Alternatively, use two Vivaflow® 200 units inparallel and ­concentrate 5 L in under 75 minutes.
Nearly total sample recoveries can be expected with most solutions.

Pressure
indicator

Feed line

Waste

Each cassette is supplied complete with tubing, pressure indicator, flow restrictor
and high-pressure pump tubing. All you need is a peristaltic pump capable ofhandling 6.4 mm OD (size 16) tubing. Should your pump head require larger
tubing, just use the interconnecter provided to attach your own peristaltic tubing
to this standard product.
To operate two cassettes in parallel, the only additional accessories needed are a
Y-connector and size 15 pump head.

Specifications
Feedreservoir

SealedPump
diafil­tration headreservoirFlowrestrictor

Vivaflow® 200
Dimensions

Overall L | W | H
Channel W | H
Active membrane area
Hold-up volume (module)
Min. recirculation volume
Non-recoverable hold-up

38 | 126 | 138 mm
10 | 0.4 mm
200 cm2
5.3 mL< 20 mL< 2 mL

Materials of construction

Main housing
Flow channel
Membrane support
Seals and O-rings
Pressure indicator
Flow restrictor
Fittings
TubingAcrylic
Acrylic
Polypropylene
Silicone
Polypropylene,
SS spring
Polypropylene
Nylon
PVC (medical grade)

Pump flow
Maximum pressure
Maximum temperature

200 to 400 mL/min
4 bar (60 psi)
60 °C

Vivaflow® 200 setup for­diafiltration

Operating conditions

42

Ultrafiltration

Protein Concentration

Performance Characteristics
Time to concentrate up to 20× [min.] at
3 bar inlet pressure, 20 °C
Start volume
1L

Average flux
[mL/min]Directrecovery [%]Recovery after
25 mL rinse [%]Lysozyme 0.25 mg/mL(14,000 MW)
2,000 MWCO Hydrosart®
3,000 MWCO PES

160
180

6
5

97 %
97 %

> 99 %> 99 %

BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
5,000 MWCO Hydrosart®
10,000 MWCO PES
10,000 MWCO Hydrosart®
30,000 MWCO PES
30,000 MWCO Hydrosart®
50,000 MWCO PES

29
70
23
35
25
20
22

33
14
41
27
38
48
43

98 %
98 %
96 %
98 %
96 %
96 %
96 %

γ Globulins 1.0 mg/mL(average 160,000 MW)
100,000 MWCO PES

54

18

96 %

99 %

Yeast 1.0 mg/mL(S. Cerevisiae)
0.2 µm PES

11

86

92 %

98 %

Dilute solute concentration,
start volume 1 L at 3 bar,
10,000 MWCO PES
BSA 0.001 mg/mL
BSA 0.01 mg/mL
BSA 0.1 mg/mL

18
20
21

52
47
45

90 %
92 %
94 %

98 %
98 %
99 %

Start volume 5 L(two Vivaflow® 200
in parallel at 3 bar)
10,000 MWCO PES
BSA 1.0 mg/mL (66,000 MW)

67

70

97 %

> 99 %----> 99 %> 99 %> 99 %> 99 %99 %> 99 %
98 %Visit us at www.sartorius.com/Vivaflow200 to get additional info.
Find instructions on how to use Vivaflow® 200 for	The measurement of soluble trace metals in seawater
The workflow in protein research laboratories
Preparation of biological nanoparticles and medical nanocarriers
Concentration and purification of viruses
Concentrating hybridoma supernatants prior to to affinity chromatography

Ultrafiltration

Protein Concentration

43

Ordering Information
Vivaflow® 200*

Qty./Pkg.

Prod. No.

3,000 MWCO PES

1

VF20P95,000 MWCO PES

1

VF20P1

10,000 MWCO PES

1

VF20P030,000 MWCO PES

1

VF20P250,000 MWCO PES

1

VF20P3100,000 MWCO PES

1

VF20P40.2 µm PES

1

VF20P72,000 MWCO Hydrosart®

1

VF20H95,000 MWCO Hydrosart®

1

VF20H1

10,000 MWCO Hydrosart®

1

VF20H030,000 MWCO Hydrosart®

1

VF20H2100,000 MWCO Hydrosart®

1

VF20H4* Vivaflow® 200 cassettes are supplied with feed, retentate and filtrate tubing, 0.6 mm flow restrictor, luer fittings and a pressure
indicator.

Vivaflow® 200 Complete Set of Accessories**
Pump (230 V), Easy-Load pump head (size 16),
tubing, 500 mL sample | diafiltration reservoir

1

VFS202

Pump (115 V), Easy-Load pump head (size 16),
tubing, 500 mL sample | diafiltration reservoir

1

VFS204* * VFS202 and VFS204 are suitable only for operation of a single Vivaflow® 200 cassette. To operate 2 cassettes in parallel, an
Easy-Load size 15 pump head is required.

Accessories

Qty./Pkg.

Prod. No.

Masterflex economy drive variable speed
peristaltic pump (240 V)

1

VFP001Masterflex economy drive variable speed
peristaltic pump (115 V)

1

VFP002500 mL sample and | or diafiltration reservoir

1

VFA006Masterflex Easy-Load pump head (size 16)1

VFA012Masterflex Easy-Load pump head (size 15)

1

VFA013Tubing and Fittings

Qty./Pkg.

Prod. No.

Size 15 tubing and Luer fittings(3 m, 4.8 mm × 2.6 mm)

1

VFA003Size 16 tubing and Luer fittings(3 m, 3.2 mm × 1.6 mm)

1

VFA004

Y-connector (size 15 to 2 × size 16 for running 2
cassettes in parallel)

1

VFA005

Flow restrictor set, 2 each of 0.4, 0.6 and 0.8 mm

6VFA009Female luer fittings, size 16

10

VFA032

Flow restrictors, 0.6 mm

6VFA035

Female luer fittings, size 15

10

VFA036

44

Ultrafiltration

Protein Concentration

Vivapore® Solvent Absorption Concentrators
3 to 20 mL Samples
With no need for additional equipment, pressure or vacuum, solvent absorption
is the most economic and user-friendly concentration technique available to theclinician and research scientist.
Just fill the unit with the solution to be concentrated, wait for the desiredconcentration level to be achieved and then pipette the concentrated sample
from the bottom of the device.
Vivapore® is ideal for general-purpose laboratory concentration and purification
prior to further analysis. It is particularly suited for labile solutions that can­denature with alternative shear- or pressure-­inducing methods or that require
processing in a cold room environment.
Vivapore® concentrators extend the solvent absorption technique to a totallynew level of performance, application potential and ease of use.

Vivapore® 5

Vivapore® solvent absorption concentrators are IVD registered devices. Article
codes are only available in IVD registered countries, according to country
specific regulations. Please contact Sartorius for more information on registeredcountries and availability.

Specifications

Vivapore® 10 | 20

Vivapore® 5

Vivapore® 10 | 20

Membrane material

PES

PES

Membrane MWCO

7,500

7,500
2

28 cm2

Membrane surface area

20 cm

Reservoir material

SAN

SAN

Volume range

1 to 5 mL

2 to 10 mL | 20 mL*

Minimum concentrate volume

50 µL

50 µL

Vivapore® overall dimensions
Width (mm)Height (mm)

42
82

46
100

* to concentrate 20 mL please use the 10 mL expansion reservoir (VPA006)

Visit us at www.sartorius.com/en/products/lab-filtration-purification/
diagnostic-sample-prep to get additional info.
Here you can find instructions on how to use Vivapore® Solvent Absorption
Concentrators and Vivaspin® products for the ­concentration of urine samples.

Ultrafiltration

Protein Concentration

45

Typical Performance Characteristics
Time to concentrate up to 10× [min.]Concentrate recovery [%]Product

VP5

VP10 | 20

VP10 | 20*

VP5

VP10 | 20

VP10 | 20*

Start volume

5 mL

10 mL

20 mL

5 mL

10 mL

20 mL

Cytochrome c (12,600 MW)

0.25
mg/mL

0.25
mg/mL

0.1
mg/mL

0.25
mg/mL

0.25
mg/mL

0.1
mg/mL

7,500 MWCO PES

35

75

150

90 %

90 %

92 %

BSA (66,000 MW) 7,500 MWCO PES

30

55

115

92 %

92 %

92 %

IgG (160,000 MW) 7,500 MWCO PES

40

70

160

75 %

77 %

78 %

* with additional reservoir

Performance Characteristics
Time to concentrate up to 50× [min.]Concentrate recovery [%]Product

VP5

VP10 | 20

VP10 | 20*

VP5

VP10 | 20

VP10 | 20*

Start volume

5 mL

10 mL

20 mL

5 mL

10 mL

20 mL

Cytochrome c (12,600 MW)
7,500 MWCO PES

65

70

160

91 %

88 %

90 %

BSA (66,000 MW) 7,500 MWCO PES

45

50

105

90 %

90 %

92 %

IgG (160,000 MW) 7,500 MWCO PES

50

65

140

53 %

65 %

74 %

* with additional reservoir

Ordering Information
Vivapore® 5*

Qty./Pkg.

Prod. No.

7,500 MWCO PES

4

VP0503**7,500 MWCO PES

30

VP0501**

100

VP0502**7,500 MWCO PES

4

VP2003**7,500 MWCO PES

30

VP2001**100

VP2002**

6

VPA002**

Plastic recovery pipettes (Vivapore 10 | 20)

100

VPA005**

10 mL expansion reservoir (Vivapore 10 | 20)

10

VPA006**

Plastic recovery pipettes (Vivapore 5)

100

VPA007**

Requires Stand
7,500 MWCO PES
Vivapore® 10 | 20*

Requires Stand
7,500 MWCO PES
Requires Stand
Disposable stands for 4 units®

®

®

* includes stand and recovery pipettes** For In Vitro Diagnostic (IVD) applications only, available in registered countries only.

46

Ultrafiltration

Protein Concentration

Ultrafiltration Membrane Filters
PES 146, CTA 145 and Hydrosart® 144

Polyethersulfone (PES)This is a general purpose membrane that provides excellent performancewith most solutions when retentate recovery is of ­primary importance. PES
membranes exhibit no hydrophobic or hydrophilic interactions and are u­ sually
preferred for their low fouling ­characteristics, exceptional flux and broad pH
compatibility.
Cellulose Triacetate (CTA)
High hydrophilicity and exceptionally low non-­specific binding characterize
this membrane. Cast without any membrane support that could trap or bindpassing micro­solutes, these membranes are ideal for sample cleaning andprotein removal, and when high recovery from the ­filtrate solution is of primary
importance.
Hydrosart®
These membranes are also highly hydrophilic and are often preferred fortheir high protein recovery when ­processing very dilute solutions. ­Resistance
to autoclaving, ease of ­cleaning and extended chemical resistance also­characterize this type of membrane.

Specifications
Specifications for Polyethersulfone, Type 146
Thickness

120 µmpH range

1 – 14

Water flux

MWCO 10,000

0.2 mL/min/cm2

Protein retention

Cytochrome C

95 %Specifications for Cellulose Triacetate, Type 145
Thickness

120 µmpH range

4–8

Water flux

MWCO 10,000

0.11 mL/min/cm2

Protein retention

Cytochrome C

90 %Specifications for Cellulose Triacetate, Type 145
Thickness

180 µmpH range

1 – 13

Water flux

MWCO 10,000

0.08 mL/min/cm2

Protein retention

Cytochrome C

99 %Ultrafiltration

Protein Concentration

47

Ordering Information
Polyethersulfone Membrane Filters, Type 146∅ in mmMWCO

Qty./Pkg.

Prod. No.

47

1,000

10

14609--47------D

63

1,000

10

14609--63------D

76

1,000

10

14609--76------D

25

5,000

10

14629--25------D

47

5,000

10

14629--47------D

63

5,000

10

14629--63------D

76

5,000

10

14629--76------D

25

10,0001014639--25------D

63

10,0001014639--63------D

76

10,0001014639--76------D

150

10,0001014639-150------D

25

30,0001014659--25------D

63

30,0001014659--63------D

76

30,0001014659--76------D

25

50,0001014650--25------D

47

50,0001014650--47------D

76

50,0001014650--76------D

25

300,000

1014679--25------D

47

300,000

1014679--47------D

76

300,000

1014679--76------D

Cellulose Triacetate Membrane Filters, Type 145∅ in mmMWCO

Qty./Pkg.

Prod. No.

25

5,000

10

14529--25------D

47

5,000

10

14529--47------D

25

10,0001014539--25------D

47

10,0001014539--47------D

50

10,0001014539--50------D

25

20,0001014549--25------D

43

20,0001014549--43------D

47

20,0001014549--47------D

47

20,00010014549--47------N

63

20,0001014549--63------D

48

Ultrafiltration

Protein Concentration

Hydrosart® Membrane Filters, Type 144∅ in mmMWCO

Qty./Pkg.

Prod. No.

25

5,000

10

14429--25------D

44

5,000

10

14429--44------D

63

5,000

10

14429--63------D

76

5,000

10

14429--76------D

25

10,0001014439--25------D

47

10,0001014439--47------D

63

10,0001014439--63------D

76

10,0001014439--76------D

25

30,0001014459--25------D

47

30,0001014459--47------D

63

30,0001014459--63------D

76

30,0001014459--76------D

50

Ultrafiltration

DNA Concentration

Vivacon® 500

For DNA Sample Desalting and Concentration
Reproducible DNA and Protein Sample Desalting and Concentration
Vivacon® 500 centrifugal concentrators offer the optimal solution for DNA andprotein concentration and buffer exchange applications. For optimal performancewith highly dilute samples, e.g. forensic samples, Vivacon® 500 incorporates
the patented regenerated cellulose membrane, Hydrosart®. High recoveries
and excellent r­ eproducibilities are combined with c­ onvenience offered by themolecular weight cutoff printed on the individual Vivacon® 500 units.
As Vivacon® 500 can be reverse spun after sample processing, this ensurescomplete concentrate recovery, which is especially important when working withlow sample concentrations.
Vivacon® 500-PCR Grade
To use DNA amplification ­technologies, any traces of DNA originating from theequipment need to be eliminated.
Vivacon® 500-PCR Grade units are treated with ethylene oxide (EtO) ina validated process to denature all traces of DNA that might interfere withsubsequent amplification procedures.
Reference: K. Shaw et al., Int. J. Legal Med. (2008) 122: 29 – 33

Specifications
Vivacon® 500
Concentrator capacity

Fixed angle rotor

0.5 mL

Dimensions

Total length (concentration)
Total length (backspin)
Width
Active membrane area
Hold-up volume of membraneand support
Dead-stop volume (40° rotor)

45 mm
47.5 mm
12.4 mm0.32 cm2

Body
Filtrate vessel
Membrane

Polycarbonate
Polypropylene
Hydrosart®,
Cellulose Acetate

Materials of construction< 5 µL
5 µL

Conversion Table for Hydrosart® MWCO to Nucleotide Cutoff
Membrane

MWCO

Double-Stranded
Nucleotide Cutoff (bp)Hydrosart®

2,000

> 10

Hydrosart®

10,000> 30

Hydrosart

30,000> 50

Hydrosart

50,000> 300

Hydrosart

100,000> 600

Cellulose Acetate

125,000

> 650

®
®
®

Ultrafiltration

DNA Concentration

51

Performance Characteristics for DNA
Start volume 0.5 mL, sample concentration 50 mg/mL.
Molecule
size (bp)Time to concentrate
up to 30× [min.]at 20 °C

Concentrate
recovery [%]

RCF
(g)

2,000 MWCO

1060 min.

93 %

7,500

10,000 MWCO30

25 min.

94 %

7,500

30,000 MWCO50

18 min.

88 %

5,000

50,000 MWCO300

18 min.

91 %

5,000

100,000 MWCO600

10 min.

87 %

3,000

125,000 MWCO650

12 min.

85 %

2,000

125,000 MWCO

900

9 min.

94 %

3,000

Performance Characteristics for ProteinsStart volume 0.5 mL, sample and concentration of proteins as specified in table.
Test moleculeTime to concentrate
up to 30× [min.]at 20 °C

Concentrate
recovery [%]

RCF
(g)

2,000 MWCO

0.25 mg/mL
cytochrome c

30 min.

95 %14,00010,000 MWCO

0.25 mg/mL
cytochrome c

15 min.

92 %14,00030,000 MWCO

1.0 mg/mL BSA

10 min.

95 %14,00050,000 MWCO

1.0 mg/mL BSA

10 min.

92 %14,000100,000 MWCO

1.0 mg/mL
bovine IgG

11 min.

90 %

8,000

125,000 MWCO

1.0 mg/mL
bovine IgG

10 min.

81 %

8,000

Vivacon® 500

Qty./Pkg.

Prod. No.

2,000 MWCO

25

VN01H91

2,000 MWCO

100

VN01H92

10,000 MWCO

25

VN01H01

10,000 MWCO

100

VN01H02

30,000 MWCO

25

VN01H2130,000 MWCO

100

VN01H2250,000 MWCO

25

VN01H3150,000 MWCO

100

VN01H32

100,000 MWCO

25

VN01H41

100,000 MWCO

100

VN01H42

125,000 MWCO

25

VN01H81

125,000 MWCO

100

VN01H82

Ordering Information

52

Ultrafiltration

DNA Concentration

Vivacon® 500-PCR Grade

Qty./Pkg.

Prod. No.

30,000 MWCO

100

VN01H22ETO

30,000 MWCO

500

VN01H23ETO

50,000 MWCO

100

VN01H32ETO

100,000 MWCO

100

VN01H42ETO

100,000 MWCO

500

VN01H43ETO

125,000 MWCO

100

VN01H82ETO

125,000 MWCO

500

VN01H83ETO

125,000 MWCO

100

VN01H82ETO

Accessories

Qty./Pkg.

Prod. No.

Collection tube

100

VNCT01

Visit us at www.sartorius.com/en/products/lab-filtration-purification/
ultrafiltration-devices/centrifugal to get additional info.
Find instructions on how to use Vivacon® 500 for--	Primer removal after a PCR reaction	Filter aided sample preparation (FASP) for proteomic ­analysis
by mass spectrometry

Ultrafiltration

DNA Concentration

53

Vivacon® 2

For DNA Sample Desalting and Concentration
Reproducible DNA Sample Desalting and Concentration
Vivacon® 2 centrifugal concentrators offer the optimal solution for DNA andprotein concentration and buffer exchange applications. For optimal
performance with highly dilute samples, e.g. forensic samples, Vivacon® 2
incorporates the patented regenerated cellulose membrane Hydrosart®. High
recoveries and excellent ­reproducibilities are combined with the convenience
provided by the volume ­graduation and molecular weight cutoff printed on theindividual Vivacon® 2 units.
As Vivacon® 2 can be reverse spun after ­sample processing, this ensurescomplete ­concentrate recovery, which is especially important when working withlow sample concentrations.
Vivacon® 2-PCR GradeVivacon® 2-PCR Grade units are treated with ethylene oxide (EtO) in a validated
process to denature all traces of DNA that might interfere with subsequent
amplification procedures.

Specifications
Vivacon® 2
Concentrator capacity

Fixed-angle rotor

2 mL

Dimensions

Total length (concentration)
Total length (backspin)
Width
Active membrane area
Hold-up volume membraneand support
Dead-stop volume (25° rotor)

125 mm
115 mm
16 mm
0.95 cm2

Body
Filtrate vessel
Backspin vial
Concentrator cap
Membrane

Polycarbonate
Polypropylene
Polypropylene
Polypropylene
Hydrosart®, ­Cellulose Acetate

Materials of construction

10 µL
55 µL

Conversion Table for Hydrosart® MWCO to Nucleotide Cutoff
Membrane

MWCO

Double-Stranded
Nucleotide Cutoff (bp)Hydrosart®

2,000

> 10

Hydrosart

10,000> 30

Hydrosart

30,000> 50

Hydrosart

50,000> 300

Hydrosart®

100,000> 600

Cellulose Acetate

125,000

> 650

®
®
®

54

Ultrafiltration

DNA Concentration

Performance Characteristics for DNA
Start volume 2 mL, sample concentration 50 mg/mL.
Molecule size
(bp)Time to concentrate
up to 30× [min.]at 20 °C

Concentrate
recovery [%]

RCF
(g)

2,000 MWCO

10

120 min.

92 %

7,500

10,000 MWCO30

60 min.

94 %

5,000

30,000 MWCO

50

60 min.

95 %

2,500

50,000 MWCO300

45 min.

96 %

2,500

100,000 MWCO600

30 min.

93 %

2,500

125,000 MWCO650

30 min.

88 %

2,500

125,000 MWCO

900

30 min.

89 %

2,500

Performance Characteristics for ProteinsStart volume 2 mL, sample and concentration of proteins as specified in table.
Test moleculeTime to concentrate
up to 30× [min.]at 20 °C

Concentrate
recovery [%]

RCF
(g)

2,000 MWCO

0.25 mg/mL
cytochrome c

120 min.

95 %

7,500

10,000 MWCO

0.25 mg/mL
cytochrome c

90 min.

96 %

5,000

30,000 MWCO

1.0 mg/mL BSA 40 min.

96 %

5,000

50,000 MWCO

1.0 mg/mL BSA 30 min.

94 %

5,000

100,000 MWCO

1.0 mg/mL
bovine IgG

30 min.

92 %

5,000

125,000 MWCO

1.0 mg/mL
bovine IgG

27 min.

81 %

5,000

Ultrafiltration

DNA Concentration

55

Ordering Information
Vivacon® 2

Qty./Pkg.

Prod. No.

2,000 MWCO

25

VN02H91

2,000 MWCO

100

VN02H92

10,000 MWCO

25

VN02H01

10,000 MWCO

100

VN02H02

30,000 MWCO

25

VN02H2130,000 MWCO

100

VN02H2250,000 MWCO

25

VN02H3150,000 MWCO

100

VN02H32

100,000 MWCO

25

VN02H41

100,000 MWCO

100

VN02H42

125,000 MWCO

25

VN02H81

125,000 MWCO

100

VN02H82

Vivacon® 2-PCR Grade

Qty./Pkg.

Prod. No.

30,000 MWCO

100

VN02H22ETO

30,000 MWCO

500

VN02H23ETO

50,000 MWCO

100

VN02H32ETO

50,000 MWCO

500

VN02H33ETO

100,000 MWCO

100

VN02H42ETO

100,000 MWCO

500

VN02H43ETO

125,000 MWCO

100

VN02H82ETO

125,000 MWCO

500

VN02H83ETO

Ultrafiltration

Protein Purification

57

Vivapure®

Ion Exchange Protein Purification Products
Fast and Easy-to-Use Spin Columns
Vivapure® Ion Exchange (IEX) spin columns are centrifugal devices
that incorporate ­Sartobind® membrane adsorber technology as theirchromatography matrix. Vivapure® IEX spin columns make protein ­purification
as easy as filtration. The devices are ready to use and eliminate the risk of r­ unningdry. For many protein purification ­applications, they replace time-consuming andtedious column chromatography.

Chromatography gel beads (right)
are shown on top of a membraneadsorber in this SEM. The
membrane adsorber pores are
more than 50 times larger thanbead pores.

The rapid Vivapure® IEX bind-wash-elute protocol especially lends itself toscreening applications, where many different samples are processed in parallel.
The Sartobind® Membrane Adsorber Matrix
Sartobind® IEX membrane adsorbers are based on stabilized regenerated
cellulose and exhibit a microporous structure with a pore size of > 3 µm, which isorders of magnitude larger than conventional chromatographic gel materials.
This allows molecules to be transported to the ligands immobilized on themembrane adsorber by convective flow, resulting in ­exceptionally high flow
rates.
By contrast, gel chromatography is slowed down due to diffusion limitations, as
the molecules need to enter the small bead pores in order to be bound by theligands. The porous membrane adsorber enables fast, reproducible and scalable­protein purification.

---

Fast and Simple-to-Use Spin
Columns	Devices are ready to use	They make protein purification
as simple as filtration

Reproducible Results	No column packing necessary as
the devices are ready to use	Membrane adsorber spin columns
cannot crack or run dry

Spin

Original
sample-Centrifugal Devices
	Offer the possibility of working
in parallel

Low Bed Volume
	Allow working with lower buffer
volumes, resulting in concentrated­elate fractions
Scalable Product Range
	Process-scale modules are available
with the same S­ artobind® IEX
membrane adsorber matrices

Spin

Protein is boundto membrane

Wash

Elute

Fast and easy protein purification with Vivapure® spin columns

Pure
protein58

Ultrafiltration

Protein Purification

Specifications
Available Formats----

Vivapure® IEX Products

Application

Vivapure® Mini spin columns

Sample fractionation Purification condition scouting
Small-scale purification

Vivapure® Maxi spin columns
Vivapure® Mini: 400  µL
Binding capacities: 1 to 4 mg

 Large-scale sample fractionation One-step protein purification | concentration Polishing of His-tagged protein

Membrane Availability

Vivapure® Maxi-19
Binding capacities: 15 to 80 mg

Functional Groups

Ion Exchanger Type

Sulfonic acid (S)Strong acidic cation exchanger: R-CH2-SO3-Na+

Quaternary ammonium (Q)Strong basic anion exchanger:

R-CH2-N+-(CH3)3Cl-

Diethylamine (D)Weak basic anion exchanger:

R-CH2-NH+-(CH2H5)2

Performance Characteristics
Vivapure® Spin Columns

Protein Binding
Capacity* (mg)Max. Volume perCentrifuge Run
Using a Swing-Out
Rotor (mL)Vivapure® Mini H

4

0.4

Vivapure Maxi H

60 to 8019®

Max. Volume per­Centrifuge Using a
Fixed-Angle
Rotor Run (mL)

10.5

* Actual yields depend on specific protein sample and selected pH and salt conditions.
Yields established using 1 mg/mL BSA in 25 mM Tris/HCL pH 8.0 with Vivapure® Q & D spin columns and 1 mg/mL cytochrome cin 25 mM sodium acetate buffer pH 5.5 with Vivapure® S spin columns.

Ordering Information
Vivapure® Mini Ion Exchange Spin Columns (up to 0.5 mL)
Description

Spin Columns

Centrifuge Tubes

Prod. No.

Vivapure® Mini S&Q H starter kit

16

32

VS-IX01SQ16

Vivapure® D Mini H

24

48

VS-IX01DH24

Vivapure® Q Mini H

24

48

VS-IX01QH24

Vivapure® S Mini H

24

48

VS-IX01SH24

Vivapure® Maxi Ion Exchange Spin Columns (up to 20 mL)
Vivapure® D Maxi H

816

VS-IX20DH08®

Vivapure Q Maxi H

816

VS-IX20QH08

Vivapure S Maxi H

816

VS-IX20SH08®

Ultrafiltration Virus Purification and Concentration

59

Vivapure® Virus Purification
and Concentration Kits
Recombinant virus vectors are the ­preferred method for a wide range of genedelivery applications. Especially ­adenovirus type 5 and VSV-G pseudotyped lentivirus are two frequently utilized viral vectors for in vitro and in vivo applications.
Recombinant Adenovirus ­Vectors
Recombinant adenovirus ­vectors are ­versatile tools in research and therapeutic
applications for gene transfer and protein expression in cell lines that have low­transfection efficiency with liposomes. After entering cells, the virus remains
epichromosomal – i.e., does not integrate into the host chromosome, leaving
the host genome unaffected. The delivery of RNAi into cells is becoming a major
application for adenovirus vectors.
Lentivirus Vectors
Lentivirus vectors are frequently used in gene transfer studies, due to their abilityof gene transfer and integration into ­dividing and non-dividing cells. The­pseudotyped envelope with vesicular ­stomatitis virus envelope G (VSV-G) protein broadens their target cell range. Lentiviral vectors have been shown to deliver genes into cell types (e.g. neurons, lymphocytes and macrophages) which
other retrovirus vectors could not be used for. The lentivirus vector is increasingly
used to integrate siRNA efficiently in a wide variety of cell lines and primary cells,
both in vitro and in vivo.
Rapid Virus Purification by Membrane Chromatography
The Sartobind® ion exchange membrane adsorber technology used in AdenoPACK and LentiSELECT is unique in its capability to efficiently and rapidly
capture and recover large virus particles. Compared with chromatography media, membrane adsorbers provide large 3,000 nm pores, allowing unrestricted
access and recovery of virus from the charged adsorber surface. Convective flow
through the syringe filter devices provides high-speed separations not possible
with traditional ­chromatography, cesium chloride density gradients and ultracentrifugation methods.
Sartorius membrane adsorbers with porous matrices, high capacities, low­differential pressures, high flow rates and low ­unspecific adsorption show excellent ­performance in small-scale virus ­purification. In addition, these syringe
filter devices are scalable and comply with cGMP requirements for large-volume,
high-performance separation, reducing final process time ten-fold.

60

Ultrafiltration Virus Purification and Concentration

Adenovirus Purification with Vivapure®
AdenoPACK Kits
AdenoPACK 20 | 100 | 500
The AdenoPACK adenovirus purification and concentration kits offer
researchers who need to recover up to 3 × 1013 purified recombinant adenovirus
particles for in vitro transfection a fast, safe and easy-to-use solution. The kits
include all reagents and devices necessary for clarification, purification andconcentration of a
­ deno­virus type 5 from HEK293 cell ­cultures – all within just
two hours. These straightforward kits replace time-­consuming and laborintensive 48-hour CsCl density gradients.
AdenoPACK kits are offered as AdenoPACK 20, AdenoPACK 100 and
AdenoPACK 500 for the purification and concentration of adenovirus type5 from 20 to 500 mL cell cultures, resulting in 1 × 1011 to 3 × 1013 ­purified viralparticles. For each sample ­volume, the most convenient handling method isprovided for ultimate ­convenience.
To this end, preparations using AdenoPACK 20 are supplied in a spin column
format for centrifuges. AdenoPACK 100 is a manually operated kit in a syringe
filter format* and ­AdenoPACK 500 is a pump-driven kit.

AdenoPACK Advantages--

Fast and Easy Virus Purification
	Purification completed injust 2 hours
	Convenient, over 10 × faster alternative to CsCl density gradient
Quantitative Yields
	In contrast to CsCl density gradient,
the complete cell culture is used forvirus p­ urification and not only theviral pellet-

Flexible Product Range	Applicable from initial construct­screening to large-scale virus
production

Complete Kit
	Including filtration devices, AdenoPACK units for virus purification,
Vivaspin® and all buffers
Low Endotoxin Levels
	High cell viability and infection
rates due to endotoxin levels of< 0.025 EU/mL* Vivapure® AdenoPACK 100 can be alternatively be operated with a ­laboratory pump or an infusion pump, for which protocols are­provided on our web page at www.sartorius-stedim.com. ­Additionally, the tubes and adaptors needed for these operating modes
can be ordered.

Ultrafiltration Virus Purification and Concentration

61

Specifications
Adenovirus Purification Kit Specifications
Product

AdenoPACK 20

AdenoPACK 100

AdenoPACK 500

Sample size

20 mL cell culture

20 to 200 mLof cell culture

500 mL of cell culture

Number of
purifications

6 × 20 mL

2 × 20 to 60 mL
1 × 200 mL

1 × 500 mL

Virus particles (VP) per mL

Typically up to
1 × 1011-1012

Typically up to
1 × 1013

Typically up to
3 × 1013

VP/IU

50 to 100

20 to 50

20 to 50

Processing time

Typically one hour

Typically two hours

Endotoxin level< 0.025 EU/mL< 0.025 EU/mL

Ordering Information
Vivapure® AdenoPACK 20
Vivapure® AdenoPACK 20

VS-AVPQ020

Vivapure AdenoPACK 20 RT*

VS-AVPQ022®

* AdenoPACK 20 RT does not contain Benzonase **®

Vivapure® AdenoPACK 100
Vivapure® AdenoPACK 100

VS-AVPQ101

Vivapure AdenoPACK 100 RT*

VS-AVPQ102®

AdenoPACK 100 Accessories
Pump tubing set for Vivapure® AdenoPACK 100

VS-AVPA001* AdenoPACK 100 RT does not contain Benzonase®**Vivapure® AdenoPACK 500
Vivapure® AdenoPACK 500

VS-AVPQ501

Vivapure® AdenoPACK 500 RT*

VS-AVPQ502* AdenoPACK RT-kits do not contain Benzonase®**** Benzonase® is a registered trademark of Merck< 0.025 EU/mL

62

Ultrafiltration Virus Purification and Concentration

Lentivirus Purification with the Vivapure®
LentiSELECT Kit
LentiSELECT 40 | 500 | 1000
The LentiSELECT kits for lentivirus purification and concentration offer researchers who need to recover up to 5 × 109 infective lentivirus particles per mL for invitro t­ ransfection or animal studies a fast and easy-to-use solution.
These straightforward kits replace time-consuming ultracentrifugation protocols, which typically take approximately one day for large sample volumes.
Vivapure® ­LentiSELECT thus reduces purification time to just a few hours.
LentiSELECT kits are offered as LentiSELECT 40, LentiSELECT 500 and
LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped
lentivirus from 40 to 1,000 mL cell cultures, resulting in 8 × 108 to 1 × 1010 purified
infective p­ articles. The most convenient handling method is provided for eachsample ­volume. To this end, 40 mL sample volumes are processed manually with
LentiSELECT 40, while LentiSELECT 500 and 1000 are pump-driven kits.

LentiSELECT Advantages-

Fast and Easy Virus Purification
	Purification completed in lessthan one to six hours, dependingon sample volume	Kit is as easy to use as filtration
No Need for Expensive Instruments
	Lentivirus purification with
LentiSELECT is independent ofequipment, such as ultracentrifuges
High Virus Purity	Achieve pure virus based on a­chromatographic method for your­experiments instead of a crude and­variable cell culture supernatantpellet-Optimal for Multiple Virus
Construct Screening	With LentiSELECT 40, four
purification runs can be conductedin parallel with one kit

Complete Kits
	Including LentiSELECT units forvirus purification, Vivaspin® units for­concentration | buffer exchange andall buffers and syringes necessary
Low Endotoxin Levels
	High cell viability and infection
rates due to endotoxin levels of< 0.025 EU/mL* Vivapure® AdenoPACK 100 can be alternatively be operated with a ­laboratory pump or an infusion pump, for which protocols are­provided on our web page at www.sartorius-stedim.com. ­Additionally, the tubes and adaptors needed for these operating modes
can be ordered.

Ultrafiltration Virus Purification and Concentration

63

Specifications
Product

LentiSELECT 40

LentiSELECT 500

LentiSELECT 1000

Sample size

40 mL cell culture

500 mL ofcell culture

1,000 mL ofcell culture

Number of ­purifications

4 × 40 mL

1 × 500 mL

1 × 1,000 mL

Virus particles (VP) per mL

Typically up to
3 × 109

Typically up to2 – 5 × 109

Typically up to4 – 6 × 1013

VP/IU

5 to 15

5 to 15

20 to 50

Processing time

Typically up to
45 minTypically up to
3 hours

Typically up to
6 hours

Endotoxin level< 0.025 EU/mL< 0.025 EU/mL< 0.025 EU/mL

Ordering Information
Vivapure® LentiSELECT 40
Vivapure® LentiSELECT 40

VS-LVPQ040

Vivapure® LentiSELECT 500
Vivapure® LentiSELECT 500

VS-LVPQ500

Vivapure® LentiSELECT 1000
Vivapure® LentiSELECT 1000

VS-LVPQ1000

65

Filtration Devices
Table of Contents

Filtration Device Portfolio Introduction
Minisart® Standard Selection Guide
Minisart® PP Standard Syringe Filter
Minisart® NML Standard Syringe Filters Medical Device CE-Minisart® Syringe Filters
Claristep® Syringeless Filtration 
Sartolab® P20 and Sartolab® P20 Plus 
Sartolab® RF | BT 
NEW! Sartolab® RF 50 
NEW! Sartolab® Multistation 
Sartoclear Dynamics® Lab P15 
NEW! Sartoclear Dynamics® Lab V 
Chemical Compatibility 

66
67
68
74
80
83
87
91
94
96
98
100
104

66

Filtration Devices

Introduction

Introduction
Syringe filters are used for many routine preparation steps
in laboratories all over the world. They are ­convenient,
ready-to-use disposables for sterile filtration of liquids andremoval of particles from solutions and gases. Dependingon the reagents filtered, syringe filters have to fulfill
certain requirements to best serve ­customer’s application.
­Sartorius offers Minisart® syringe filters and filters optimized
for a wide range of relatively large volumes. The filters arereliably remove particles with no leakage. If you need torely on the quality of your filtrate – whether it needs to besterile prior to use or ­particulate-free before analysis – fieldproven, high-quality Sartorius filter syringes are the No. 1
choice for reliable, convenient preparation steps.

Sartorius has developed a new, easy-to-use andstraightforward filtration setup. The manually operated
Claristep® Filtration System consisting of a station and filter
units offers a novel way for clarifying your samples prior to
analysis.

Our Product Range
For clarification and sterilization of liquids, filtration is the
optimal method. It removes microorganisms and particles
reliably, without any effects on the ingredients due toadsorption or decomposition. For optimal results, Minisart®
NML Standard syringe filters with an MBS housing provide
a choice of membranes with pore sizes ranging from 0.1
μm to 5 μm for high flow rates and the low adsorption
characteristics. The effective filtration area of 6.2 cm2 for the
fast filtration is the largest among premium syringe filters
available, and the MBS housing is color- coded for easy
pore size identification. For a list of the types offered, pleasesee page 74.

Claristep® syringeless filter units with RC membranes are
optimized for solvents and aqueous solutions. They provide
maximum chemical compatibility and exceptionally low
non-specific binding of analytes.

Elimination of particles from your samples prior to HPLCor other chromatographic analysis is essential in order tomaintain the integrity of your chromatography column andto maximize its operating lifetime. Minisart® PP Standard
syringe filters optimized for sample preparation consistof a polypropylene housing and membrane componentsfeaturing maximum chemical compatibility and minimum
extractables to ensure excellent results. Due to the typicalrange of volumes from less than 1 mL to 100 mL, thesefilters are available in three different diameters with aneffective filtration area of 0.07 cm2, 1.7 cm2 and 4.8 cm2.
For a selection guide, please see page 67..
The Sartorius medical device CE-Minisart® syringe filter
with a hydrophilic (surfactant-free) cellulose acetate andhydrophobic poly­tetrafluoroethylene (PTFE) are theperfect choice for pharmacy admixture applications likesterile filtration and | or clarification of low volume solutions
in a laboratory environment before use for patient care. The
CE-Minisart® syringe filters are manufactured by Sartorius
in a facility whose Quality Management System is certified
for compliance with EN ISO 13485 (see page 80).
Minisart® Standard Syringe Filters are not for medical use.

Claristep® Filter units are processed without syringe and aremade of the purest materials. Another major benefit is thatthe contact time of the samples with the filters and the caps
is extremely short, ensuring optimal, contamination-free
results. The Claristep® Station consists of a base, a lid andan exchangeable tray for easy and accurate positioning ofsample vials and Claristep® Filter units.

Sartolab® vacuum filtration devices with 0.1 µm and 0.22 µm
PES membranes for convenient filtration of 150 mL up to
1 L are ready to use and sterile. ­Sartolab® RF is a completesys­tem that includes a receiver flask. Sartolab® BT Sterile
is a bottle top filter without a receiver flasks. This enables
customers to use a receiver bottle of their choice and toeven expand filtration capacity, depending on the particle
load of the filtered liquid by filling more than one receiverflask. Sartolab® 150V is a disposable vacuum filter with apleated 0.22 µm PES membrane, which is suitable for up
to 15 L of liquid.
Sartolab® P20 pressure filtration devices with a 0.2 µm and0.45 µm PES membrane are available with or without a glass
fiber prefilter, depending on your needs. Sartolab P20 isdesigned for up to 3 L volumes and can also be used in-line.
The polycarbonate housing and membrane components
are ideal for filtering liquids. The glass fiber prefilter types are
ideal for filtering environmental samples that have a highparticle load prior to analyzing such samples.

---

Typical Applications for Filtration Devices
	Sterile filtration of liquids and gases with virtually no effecton the ingredients	Particle removal from liquids and gases prior to
downstream processes
	Venting of vials, bottles, containers, bags and bioreactors
and fermenters	Removal of precipitates and coagulates from solutions
prior to use

Filtration Devices

Minisart® Syringe Filters

67

Minisart® Standard Selection Guide
Please refer to Minisart® RC, NY or SRP for the highest chemical compatibility on page 71.
Please refer to Minisart® NML or Minisart® High Flow on page 74.

Sample Composition

Aqueous

Aqueous | Solvents

All Aqueous Solutions
Buffers, Protein Analysis

All Aqueous Solutions
Tissue Culture Media

Aqueous | Solvent
Mixtures | Solvents

Solvent Mixtures |Solvents

Solvents | Gases |Acids | BasesCA
Cellulose Acetate

PES
Polyethersulfone

RC
Regenerated
CelluloseNY
Polyamide, Nylon

PTFE
Polytetrafluoro­
ethylene

Hydrophilic

Sample Volumes

Pore Sizes

Sterilization

Hydrophobic

Sample Preparation | Clarification | Particle Removal

Prefiltration

Small
Bacteria
Mycoplasma
Colloids> 0.1

UHPLC, etc.
(Columns< 3 µmParticles)
Bacteria

HPLC, etc.
(Columns> 3 µmParticles)
Particles

Particles
Yeast
Cells

Particles
Yeast
Cells

Particles
Yeast
Cells
Platelets

Large
Particles
Cells

Glass Prefilter
Glass+Membrane
HighlyParticle-laden
Samples

0.1 µm

0.2 µm0.45 µm

0.65 µm

0.8 µm

1.2 µm

5 µm

GF (Glass Fiber)

1 mL to 200 mL

1 mL to 100 mL

0.5 mL to 15 mL

0.05 mL to 1 mL

28 mm for up to 200 mL

25 mm for up to 100 mL

15 mm for up to 15 mL

4 mm for up to 1 mL

68

Filtration Devices

Minisart® Syringe Filters

Minisart® PP Standard Syringe Filter Sample
Preparation for Analytics

4 mm packages are color-coded

Male Spike Outlet

Male Luer Slip Outlet

Minisart® RC 15 mm

Minisart® NY 15 mm

Minisart® SRP 15 mm

Minisart® RC 25 mm

Minisart® NY 25 mm

Minisart® SRP 25 mm

Reliable Removal of Particles from Liquids and Gases
Particle removal by filtration before ­analysis substantially increases the lifetimeof your columns. Minisart® RC is optimized for aqueous liquids and solventsand is compatible with DMSO, other ­amides, ketones, esters and ethers. Minisart® NY is exceptionally pure ­compared with other common polyamide (=nylon)
filters and competitor products. For this product raw materials are used which do
not interfere with standard ­analytical methods.
Our coating-free hydrophobic PTFE membrane used in Minisart® SRP is suitablefor venting applications.

---

Minisart® Features	Low adsorption of analytes	Maximum chemical compatibility
	Minimum extractables

Filtration Devices

Minisart® Syringe Filters

HPLC Certification
mAU
70

Methanol60

Filtered with Minisart® SRP
Filtered with Minisart®NY
Filtered with Minisart® RC
Unfiltered Methanol

50
40
30
20
10
0

-10

0

10

20

30

mAU
70

40

50

60 minAcetonitrile

60

Filtered with Minisart® SRP
Filtered with Minisart®NY
Filtered with Minisart® RC
Unfiltered Acetonitrile

50
40
30
20
10
0
-10

0

10

20

30

mAU
70

40

50

60 minWater

60

Filtered with Minisart® PES
Filtered with Minisart® NML
Unfiltered Water

50
40
30
20
10
0
-10

0

10

20

30

40

50

60 minHPLC Procedure
Column C18: 250 × 4.6 mm, Flow Rate: 1 mL/min, Wavelength: 220 nm
HPLC Injection Volume: 20 µL, Analysis Time: 65 min, Temperature: 40 °C,
Mobile Phases: A) Acetonitrile | B) Water, Gradient: Hold 60 % A for 10 min,
60 % to 95 % A in 20 min, 95 % to 100 % A in 35 min

69

70

Chapter Devices
Filtration

Minisart® Syringe Filters

Minisart® with Polypropylene Housing

Specifications
Minisart® RC | SRP | NY | PES with 4 | 15 | 25 mm ∅ Membrane Filtration Area
Housing material

Polypropylene (PP)

Membranes

RC = Regenerated Cellulose | NY = Polyamide |SRP = Hydrophobic PTFE = Polytetrafluoroethylene | PES = Polyethersulfone |PES - = hydrophobic PES

Glass fiber prefilter

NY Plus: Ultrapure quartz, 0.7 µm particle retention

Max. operating pressure

4.5 bar | 65 psi
Minisart® PES - : 2.0 bar | 29 psi (IN - OUT) or 0.5 bar | 7 psi (OUT - IN)

Housing burst pressure≥ 7 bar | 102 psi

Max. temperature

60 °C

Sterilization

Non-sterile Minisart® can be autoclaved or sterilized by ethylene oxide (EO)

Minisart® Membrane Types

RC 0.2 µm

RC 0.2 µm

RC 0.45 µm

SRP 0.2 µm

SRP 0.45 µm

Non-sterile packs: 50 (K), 200 (S),500 (Q), 1000 (R) | sterile packs:
individually packaged, 50 (ACK)

K|S|Q|R

ACK

K|S|Q|R

K | S | Q | ACK

K|S|Q

Bubble point (≥)

With water
3.0 bar | 44 psi

With water
4.6 bar | 67 psiWith water
2.0 bar | 29 psi

With ethanol
1.1 bar | 16 psiWith ethanol
0.9 bar | 13 psi----

Flow rate ((≥) mL/min), 4 mm ∅ = 0.07 cm2 filter area | Hold-up volume1: ≤ 10 µL
For water at 1 bar

0.5

–

1.5

–3

–3

For methanol at 1 bar

1.5

–

3.0

2.0

4.5

For air at 0.1 bar

–2

–

–2

30

60

Flow rate ((≥) mL/min), 15 mm ∅ = 1.7 cm2 filter area | Hold-up volume1: ≤ 100 µL
For water at 1 bar

20

10

40

–3

–3

For methanol at 1 bar

55

25

105

55

150

For air at 0.1 bar

–2

–2

–2

800

1,600

Flow rate ((≥) mL/min), 25 mm ∅ = 4.8 cm2 filter area | Hold-up volume1: ≤ 200 µL
For water at 1 bar

80

50

160

–3

–3

For methanol at 1 bar

160

90

325

60

260

For air at 0.1 bar

–2

–2

–2

1,800

3,000

Water penetration point3 (≥)

–

–

–

4.0 bar | 58 psi

3.0 bar | 44 psi

Sterile filtration capability5 acc.
to the bacteria challenge test

No

Yes

No

Yes

No

Non-pyrogenic according
to the USPFiltration Devices

Minisart® Syringe Filters

71

Minisart® Membrane Types

NY 0.2 µm

NY 0.45 µm

NY Plus 0.2 µm

NY Plus 0.45 µm PES 0.2 µm

PES -0.2 µm

Non-sterile packs: 50 (K), 200 (S),500 (Q), 1000 (R) | sterile packs:
individual packaged, 50 (ACK)K | Q | R | ACK

K | Q | R | ACK

K|Q

K|Q

K | Q | ACK

K|Q

Bubble point (≥)

With water
3.0 bar | 44 psi

With water
2.0 bar | 29 psi

With water
3.0 bar | 44 psi

With water
2.0 bar | 29 psi

With water
3.2 bar | 46 psiWith ethanol
0.95 bar | 14 psi----

Flow rate ((≥) mL/min), 4 mm ∅ = 0.07 cm2 filter area | Hold-up volume1: ≤ 10 µL
For water at 1 bar

–

–

–

–

1.5

–

For methanol at 1 bar

–

–

–

–

–4

–

For air at 0.1 bar

–

–

–

–

–2

–

Flow rate ((≥) mL/min), 15 mm ∅ = 1.7 cm2 filter area | Hold-up volume1: ≤ 100 µL
For water at 1 bar

20

40

–

–

40

–

For methanol at 1 bar

40

110

–

–

–4

–

For air at 0.1 bar

–2

–2

–

–

–2

–

Flow rate ((≥) mL/min), 25 mm ∅ = 4.8 cm2 filter area | Hold-up volume1: ≤ 200 µL
For water at 1 bar

50

100

50

100

100

–

For methanol at 1 bar

70

200

70

200

–4

–4

For air at 0.1 bar

–2

–2

–2

–2

–2

1,200

Water penetration point3 (≥)

–

–

–

–

–

2.0 bar | 29 psi

Sterile filtration capability5 acc.
to the bacteria challenge text

Yes

No

Yes

No

Yes

Yes

Non-pyrogenic according
to the USP
1 Hold-up volume after air purge
2 Hydrophilic membranes can filter dry air or gas but become impermeable to air or gas when wetted!
3 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point or pre-wet them using
an organic solvent (e.g. ethanol).
4 PES is suitable for solutions only containing up to 30 % MeOH.
5 According to the bacterial challenge test (BCT) with ≥ 1 × 107 cfu/cm2 Brevundimonas diminuta. Non-sterile RC Minisart® types are optimized for sample ­preparation and are not suitable for sterile
filtration according to the bacteria challenge test. All other non-sterile Minisart® types with 0.2 µm pore size can be sterilized by autoclaving or EO before use for sterile filtration.
6 For sterile packs ACK.
Minisart® Standard Syringe Filters are not for medical use.

72

Filtration Devices
Chapter
Minisart® Syringe Filters

Sample Preparation for Chromatography

Ordering Information
Minisart® RC (Regenerated Cellulose)∅ in mm | EFA1

Membrane

Housing

Pore Size

Connector Outlet

Color | Printing

Sterile*Qty./Pkg.

Order No.

25 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

Yes

50

17764--------ACK

25 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

No

5017764----------K

25 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

No

200

17764----------S

25 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

No

500

17764----------Q

25 mm

RC

PP

0.45 µm

Male Luer SlipWhite, Printed

No

5017765----------K

25 mm

RC

PP

0.45 µm

Male Luer SlipWhite, Printed

No

200

17765----------S

25 mm

RC

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

17765----------Q

15 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

Yes

50

17761--------ACK

15 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

No

5017761----------K

15 mm

RC

PP

0.2 µmMale Luer SlipWhite, Printed

No

500

17761----------Q

15 mm

RC

PP

0.45 µm

Male Luer SlipWhite, Printed

No

5017762----------K

15 mm

RC

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

17762----------Q

4 mm

RC

PP

0.2 µmMale Luer SlipBlue Tray

No

50

17821----------K

4 mm

RC

PP

0.2 µmMale Luer SlipBlue Tray

No

500

17821----------Q

4 mm

RC

PP

0.45 µm

Male Luer Slip

Yellow Tray

No

50

17822----------K

4 mm

RC

PP

0.45 µm

Male Luer Slip

Yellow Tray

No

500

17822----------Q

Minisart® SRP (Hydrophobic PTFE)
25 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

Yes

50

S7575------FXOSK

25 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

No5017575----------K

25 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

No

200

17575----------S

25 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

No

500

17575----------Q

25 mm

PTFE

PP

0.2 µmHose Barb

White, Printed

No

500

1757A----------Q

25 mm

PTFE

PP

0.45 µm

Male Luer SlipWhite, Printed

No5017576----------K

25 mm

PTFE

PP

0.45 µm

Male Luer SlipWhite, Printed

No

200

17576----------S

25 mm

PTFE

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

17576----------Q

15 mm

PTFE

PP

0.2 µmMale SpikeWhite, Printed

No5017558----------K

15 mm

PTFE

PP

0.2 µmMale SpikeWhite, Printed

No

500

17558----------Q

15 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

Yes

50

17573--------ACK

15 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

No5017573----------K

15 mm

PTFE

PP

0.2 µmMale Luer SlipWhite, Printed

No

500

17573----------Q

15 mm

PTFE

PP

0.45 µm

Male SpikeWhite, Printed

No5017559----------K

15 mm

PTFE

PP

0.45 µm

Male SpikeWhite, Printed

No

500

17559----------Q

15 mm

PTFE

PP

0.45 µm

Male Luer SlipWhite, Printed

No5017574----------K

15 mm

PTFE

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

17574----------Q

4 mm

PTFE

PP

0.2 µmMale Luer SlipBlue Tray

No

500

17844----------Q

4 mm

PTFE

PP

0.45 µm

Male Luer Slip

Yellow Tray

No

50

17820----------K

4 mm

PTFE

PP

0.45 µm

Male Luer Slip

Yellow Tray

No

500

17820----------Q

Filtration Devices

Minisart® Syringe Filters

73

Minisart® NY (Nylon) and NY25 Plus (Glass Fiber 0.7 µm2 + Nylon)∅ in mm | EFA1

Membrane

Housing

Pore Size

Connector Outlet

Color | Printing

Sterile*Qty./Pkg.

Order No.

25 mm

Nylon

PP

0.2 µmMale Luer SlipWhite, Printed

Yes

50

17845--------ACK

25 mm

Nylon

PP

0.2 µmMale Luer SlipWhite, Printed

No

500

17845----------Q

25 mm

Nylon

PP

0.45 µm

Male Luer SlipWhite, Printed

Yes

50

17846--------ACK

25 mm

Nylon

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

17846----------Q

15 mm

Nylon

PP

0.2 µmMale Luer SlipWhite, Printed

No

50

1776B----------K

15 mm

Nylon

PP

0.2 µmMale Luer SlipWhite, Printed

No

500

1776B----------Q

15 mm

Nylon

PP

0.45 µm

Male Luer SlipWhite, Printed

No

50

1776C----------K

15 mm

Nylon

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

1776C----------Q

25 mm

GF+NylonPP

0.2 µmMale Luer SlipWhite, Printed

No

50

1784B----------K

25 mm

GF+NylonPP

0.2 µmMale Luer SlipWhite, Printed

No

500

1784B----------Q

25 mm

GF+Nylon

PP

0.45 µm

Male Luer SlipWhite, Printed

No

50

1784C----------K

25 mm

GF+Nylon

PP

0.45 µm

Male Luer SlipWhite, Printed

No

500

1784C----------Q

Minisart® PES (Polyethersulfone) Aqueous Filtration
15 mm

PES

PP

0.22 μm

Male Luer Slip

White

Yes

50

1776D--------ACK

15 mm

PES

PP

0.22 μm

Male Luer Slip

White

No

500

1776D----------Q

Minisart® PES- (Hydrophobic PES) Venting & Gas Filtration, Gamma Stable
25 mm

PES

PP

0.2 μmMale Luer SlipWhite, Printed

No

50

1757H----------K

25 mm

PES

PP

0.2 μmMale Luer SlipWhite, Printed

No

500

1757H----------Q

25 mm

PES

PP

0.2 μmHose Barbs3

White, Printed

No

50

1757G----------K

PES

PP

0.2 μmHose Barbs3

White, Printed

No

500

1757G----------Q

25 mm* Sterile Minisart syringe filters are individually packaged. If not stated otherwise, Minisart units have been sterilized by ethylene oxide.
Non-presterilized Minisart® units: RC, PTFE and nylon can be sterilized by autoclaving at 121 °C for 30 min. or by using ethylene oxide (EO).
1 Diameter of EFA – Effective Filtration Area
2 0.7 µm = GF particle retention ≠ pore size!
3 Hose barbs, inlet and outlet, stepped 4.4–6 mm diameter®

®

Minisart® Standard Syringe Filters are not for medical use.

For technical product specifications, please see page 70.

74

Filtration Devices

Minisart® Syringe Filters

Minisart® NML Standard Syringe Filter
Clarification and Sterilization by Filtration
0.1

0.45

0.22

Filtration is the Optimal Method for Clarification and Sterilization of Liquids
and Gases
Sterilization by filtration is the fastest method for removal of bacterial cells fromliquids, while minimizing the effects on ­ingredients. Minisart® NML with (surfactant-free) cellulose acetate (SF)CA is the best choice for all aqueous solutions
with a pH of 4 to 8. It combines fast flow rates and is available in many d­ ifferent
pore sizes – also for the removal of larger particles. Minisart® High Flow with polyethersulfone (PES) is optimal for delivering the highest flow rates and for a broad
pH compatibility range from 1 to 13. Due to the asymmetric membrane structure,
the PES surface almost behaves like a prefilter.

Minisart® High Flow with PES

Both Minisart® types – NML and High Flow – are available pre-sterilized byethylene oxide (EO) or gamma irradiation. Hydrophobic PTFE filters like Minisart®
SRP are suitable for venting purposes and are additionally available in special
formats with activated carbon.
5.0

1.2

0.8

0.65

0.45

0.2

Minisart® NML with (SF)CA

0.2

Minisart® HY with PTFE--

Minisart® Features
Largest effective filtration
area (EFA) of 6.2 cm2	Low adsorption
High flow rate

28 mm EFA
33 mm housing diameter(for NML and High Flow)---	High total throughput
	Low hold-up volume
	Gamma-irradiated or EO-sterilized

75

76

Filtration Devices

Minisart® Syringe Filters

Minisart® Standard Syringe Filters with MBS Housing

Specifications
Minisart® High Flow | NML | NML Plus with 28 mm accessible membrane filtration area diameter, ≤ 150 µL hold-up volume1
Minisart® HY | Acticosart with 26 mm accessible membrane filtration area diameter, ≤ 150 µL hold-up volume1
Minisart® Air with 15 mm accessible membrane filtration area diameter, ≤ 100 µm hold-up volume1
Housing material

Methacrylate butadiene styrene (MBS)MembranesHigh Flow: PES = Polyethersulfone
NML: (SF) CA = (Surfactant-free) Cellulose Acetate
NML Plus: (SF) CA = (Surfactant-free) Cellulose Acetate
HY | Acticosart | Air: Hydrophobic PTFE = Polytetrafluoroethylene

Glass fiber prefilter

NML Plus: Binder-free GF, 0.7 µm particle retention

Max. operating pressure

High Flow: 6.0 bar | 87 psi
NML, NML Plus, HY, Air: 4.5 bar | 65 psi
Acticosart: 1 bar | 14.5 psi

Housing burst pressure≥ 7 bar | 102 psi (not determined for Acticosart)Max. temperature

60 °C

Sterilization

Non-sterile Minisart® High Flow, NML and NML Plus can be or sterilized by ethylene oxide (EO)
or by gamma irradiation.
Non-sterile Minisart® HY, Acticosart, Air* can be sterilized by ethylene oxide (EO).

Minisart® Membrane Types

PES
0.1 µmPES
0.2 µm

PES
0.45 µm

SFCA
0.2 µm

SFCA
0.45 µm

CA0.65 µm

CA
0.8 µmCA
1.2 µmCA
5.0 µmNon-sterile packages:
500 (Q, HYQ), 1000 (R),sterile packs: individually packaged:
50 (K, GUK, HYK, HNK)

K

K|
GUK |
Q

K|
GUK |
Q

K|
GUK |
Q

K|
GUK |
Q

K

K|
GUK |
Q

K|Q

K|Q

Bubble point (≥)

With water With water With water With water With water With water With water With water With water
5.0 bar |3.2 bar |2.0 bar |3.2 bar |2.0 bar |1.3 bar |0.8 bar |0.7 bar |0.4 bar |73 psi46 psi29 psi46 psi29 psi19 psi12 psi10 psi
6 psi

Flow Rate for 2 | 3 (≥ mL/min)
28 mm ∅ for water at 1 bar

40

140

220

60

160

250

400

500

600

15 mm ∅ for air at 0.1 bar

–

–

–

–

–

–

–

–

–

26 mm ∅ for air at 0.1 bar

–

–

–

–

–

–

–

–

–

Water penetration point3 (≥)

–

–

–

–

–

–

–

–

–

Sterile filtration capability4acc. to the bacteria challenge test

Yes

Yes

No

Yes

No

No

No

No

No

Non-pyrogenic according
to the USP

Yes5

Yes5

Yes5

Yes5

Yes5

Yes5

Yes5

Yes5

Yes5

Filtration Devices

Minisart® Syringe Filters

77

Minisart® Membrane Types

GF + SFCA
0.2 µmGF + SFCA
0.45 µm

GF + CA
1.2 µmGF
0.7 µmPTFE
0.2 µmPTFE
1.0 µmActicosart

PTFE (Air)0.2 µmNon-sterile packages:
500 (Q, HYQ), 1000 (R),sterile packs: individually packaged,
50 (K, GUK, HYK, HNK)

K|Q

K|Q

Q

K|Q

K|Q

HYQ

Q

Q | HNK

Bubble point (≥)

With water
3.2 bar |46 psiWith water
2.0 bar |29 psiWith water
0.7 bar |10 psiWith water
0.5 bar |7 psiWith ethanol With ethanol With ethanol With ethanol
1.4 bar |0.5 bar |0.9 bar |1.0 bar |20 psi
7 psi
13 psi14 psi

28 mm ∅ for water at 1 bar

60

160

350

450

–

–

–

–

15 mm ∅ for air at 0.1 bar

–

–

–

–

–

–

–

800

26 mm ∅ for air at 0.1 bar

–

–

–

–

2,000

4,000

2,300

–

Water penetration point3 (≥)

–

–

–

–

4.0 bar |58 psi

1.5 bar |22 psiN.a.

3.2 bar |44 psi

Sterile filtration capability4
according to thebacteria challenge test

Yes

No

No

No

Yes

No

N.a.

Yes

Flow rate for 2 | 3 (≥ mL/min)

Non-pyrogenic according
to the USP

Yes5

1 Hold-up volume after air purge
2 Hydrophilic membranes can filter dry air or gas but become impermeable to air or gas when wetted!
3 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point.
4 According to bacterial challenge test (BCT) with 1 × 107 cfu/cm2 Brevundimonas diminuta. All non-sterile Minisart® types listed above can be sterilizedaccording to the method recommended in this table.
5 For sterile packs K | GUK*Minisart® Air can be sterilized by Gamma irradiation according to the following parameters: Range 25 – 40 kGy (validated with 50 kGy).
Standard Minisart® Syringe Filters are not for medical use.

78

Filtration Devices

Minisart® Syringe Filters

Preparation of Aqueous Liquids

Ordering Information
Minisart® High Flow (PES – Polyethersulfone)∅ in mm | EFA1

Membrane

Housing

Pore Size

Connector Outlet

Color | Printing

Sterile*Qty./Pkg.

Order No.

28 mm

PES

MBS

0.1 µmMale Luer Lock

Dark Red

Yes

50

16553----------K

28 mm

PES

MBS

0.22 µm

Male Luer Lock

Royal Blue

Yes#

50

16532--------GUK

28 mm

PES

MBS

0.22 µm

Male Luer Lock

Royal Blue

Yes

50

16532----------K

28 mm

PES

MBS

0.22 µm

Male Luer SlipRoyal Blue

Yes

50

16541----------K

28 mm

PES

MBS

0.22 µm

Male Luer Lock

Royal Blue

No

500

16532----------Q

28 mm

PES

MBS

0.22 µm

Male Luer SlipRoyal Blue

No

500

16541----------Q

28 mm

PES

MBS

0.45 µm

Male Luer Lock

Amber

Yes

50

16537----------K

28 mm

PES

MBS

0.45 µm

Male Luer Lock

Amber

No

500

16537----------Q

28 mm

PES

MBS

0.45 µm

Male Luer Slip

Amber

Yes#

50

16533--------GUK

28 mm

PES

MBS

0.45 µm

Male Luer Slip

Amber

Yes

50

16533----------K

28 mm

PES

MBS

0.45 µm

Male Luer Slip

Amber

No

500

16533----------Q

Minisart® NML ((SF)CA – (Surfactant-free) Cellulose Acetate)
28 mm

SFCA

MBS

0.2 µmMale Luer Lock

Blue

Yes

50

S6534------FMOSK

28 mm

SFCA

MBS

0.2 µmMale Luer Lock

Blue

Yes#

50

S6534------FMGUK

28 mm

SFCA

MBS

0.2 µmMale Luer Lock

Blue

No

500

S6534------FM--Q

28 mm

SFCA

MBS

0.2 µmMale Luer Slip

Blue

Yes

50

S7597------FXOSK

28 mm

SFCA

MBS

0.2 µmMale Luer Slip

Blue

No

500

S7597------FX--Q

28 mm

SFCA

MBS

0.45 µm

Male Luer Lock

Yellow

Yes

50

S6555------FMOSK

28 mm

SFCA

MBS

0.45 µm

Male Luer Lock

Yellow

Yes#

50

S6555------FMGUK

28 mm

SFCA

MBS

0.45 µm

Male Luer Lock

Yellow

No

500

S6555------FM--Q

28 mm

SFCA

MBS

0.45 µm

Male Luer Slip

Yellow

Yes

50

S7598------FXOSK

28 mm

SFCA

MBS

0.45 µm

Male Luer Slip

Yellow

No

500

S7598------FX--Q

28 mm

CA

MBS

0.65 µm

Male Luer Slip

Pink

Yes

50

16569----------K

28 mm

CA

MBS

0.8 µmMale Luer Lock

Green

Yes

50

16592----------K

28 mm

CA

MBS

0.8 µmMale Luer Lock

Green

Yes#

50

16592--------GUK

28 mm

CA

MBS

0.8 µmMale Luer Lock

Green

No

500

16592----------Q

28 mm

CA

MBS

1.2 µmMale Luer Lock

Red

Yes

50

17593----------K

28 mm

CA

MBS

1.2 µmMale Luer Lock

Red

No

500

17593----------Q

28 mm

CA

MBS

5 µm

Male Luer Lock

Brown

Yes

50

S7594------FMOSK

28 mm

CA

MBS

5 µm

Male Luer Lock

Brown

No

500

17594----------Q

Filtration Devices

Minisart® Syringe Filters

79

Minisart® NML Plus (Glass Fiber 0.7 µm2 + SFCA)∅ in mm | EFA1

Membrane

Housing

Pore Size

Connector Outlet

Color | Printing

Sterile*Qty./Pkg.

Order No.

28 mm

GF+SFCAMBS

0.2 µmMale Luer Lock

Blue

Yes

50

17823----------K

28 mm

GF+SFCAMBS

0.2 µmMale Luer Lock

Blue

No

500

17823----------Q

28 mm

GF+SFCAMBS

0.45 µm

Male Luer Lock

YellowYes

50

17829----------K

28 mm

GF+SFCAMBS

0.45 µm

Male Luer Lock

Yellow

No

500

17829----------Q

28 mm

GF+CA

MBS

1.2 µmMale Luer Lock

Red

No

500

17825----------Q

28 mm

GF

MBS

0.7 µm2

Male Luer Lock

White

No

50

17824----------K

28 mm

GF

MBS

0.7 µm2

Male Luer Lock

White

No

500

17824----------Q

Minisart® HY (hydrophobic PTFE), for Venting and Gas Filtration
26 mm

PTFE

MBS

0.2 µmMale Luer Lock

Clear

Yes

50

S6596------FMOSK

26 mm

PTFE

MBS

1 µm

Male Luer Lock

Clear

No

500

1659A--------HYQ

26 mm

PTFE

MBS

0.2 µmMale Luer Lock

Clear

No

500

S6596------FM--Q

Minisart® High Flow (PES – Polyethersulfone) Aqueous Filtration
28 mm

PES

MBS

0.1 µmMale Luer Lock

Dark Red

Yes

50

16553----------K

Minisart® Air (Hydrophobic PTFE) Venting
15 mm

PTFE

MBS

0.2 µmMale Luer Slip

Yellow

No

500

1751A----------Q

15 mm

PTFE

MBS

0.2 µmMale Luer Slip + Needle Yellow

No

500

1751A----------Q

No

500

17840----------Q

Minisart® Acticosart with Dome Reservoir + Hydrophobic PTFE Venting & Ultracleaning of Gases
26 mm

Active carbon

MBS0.45 µm

Male Luer SlipBlue

* Sterilized Minisart® units are individually packaged. If not stated otherwise, Minisart® are sterilized by ethylene oxide.
#-mark indicates sterilization by gamma irradiation.
Non-presterilized Minisart® units: High Flow, NML, NML Plus and HY can be sterilized by ethylene oxide; High Flow, NML and NML Plus can also be sterilized by gamma irradiation
1 Diameter of EFA – Effective Filtration Area2  0.7 µm = GF particle retention ≠ pore size!
Minisart® Standard Syringe Filters are not for medical use.

For technical product specifications, please see page 76.

80

Filtration Devices

Minisart® Syringe Filters

CE-Minisart® Syringe Filters

5.0

0.45

0.2

0.2

For medical use – Sterile Filtration and Sterile Venting
The medical device CE-Minisart® NML and Ophthalsart with(surfactant-free) cellulose acetate ((SF)CA), and CE-Minisart® HY and
SRP with hydrophobic PTFE are frequently used for sterile filtrationand | or clarification of aqueous and oily liquids and other medical
applications. CE-Minisart® NML with a 5 µm cellulose acetate (CA)membrane removes particulates or coagulates offering high total
throughput under sterile conditions. Hydrophobic PTFE filters are
suitable for venting purposes. All CE-Minisart® filters are intended to be
used in a laboratory environment before use for patient care.

---

Minisart® Features	Low adsorption	Gamma-irradiated or EO-sterilized	Biocompatible acc. to ISO 10993-1

Filtration Devices

Minisart® Syringe Filters

81

CE-Minisart® Syringe Filters

Specifications
CE-Minisart® NML / Ophthalsart with 28 mm accessible membrane filtration diameter, ≤ 150 µL hold-up volume1
CE-Minisart® HY with 26 mm accessible membrane filtration diameter, ≤ 200 µL hold-up volume1
CE-Minisart® SRP with 25 mm accessible membrane filtration diameter, ≤ 200 µL hold-up volume1
Housing material

NML/Ophthalsart/HY: Methacrylate butadiene styrene (MBS)
SRP: Polypropylene (PP)

Membranes

NML /Ophthalsart: (SF)CA = (Surfactant-free) Cellulose Acetate
NML (5 µm): CA = Cellulose Acetate
HY: Hydrophobic PTFE = Polytetrafluoroethylene
SRP: Hydrophobic PTFE = Polytetrafluoroethylene

Max. operating pressure

4.5 bar | 65 psi

Housing burst pressure≥ 7 bar | 102 psi

Max. temperature

60 °C

Sterilization

Non-sterile Minisart® NML can be sterilized by ethylene oxide (EO) or gamma irradiation.
Non-sterile Minisart® HY can be sterilized by ethylene oxide (EO)

Minisart® type with regards
to membrane

SFCA
0.2 µm

SFCA
0.45 µm

CA
5.0 µmPTFE
0.2 µmPTFE
0.2 µmNon-sterile packages:
500 (Q, HYQ),
sterile packs: individually packaged:
50 (K, GUK, HYK, ACK)

K|
GUK |
Q

K|
GUK |
Q

K

HYK |
HYQ

ACK

Bubble point (≥)

With water
3.2 bar |46 psiWith water
2.0 bar |29 psiWith water
0.4 bar |6 psiWith ethanol
1.4 bar |20 psiWith ethanol
1.1 bar |16 psi

28 mm ∅ for water at 1 bar

60

160

600

–

–

25 mm ∅ with ethanol at 1 bar

–

–

–

–

60

25 mm ∅ for air at 0.1 bar

–

–

–

–

1,800

26 mm ∅ for air at 0.1 bar

–

–

–

2,000

–

Water penetration point2 (≥)

–

–

–

4.0 bar |58 psi4.0 bar |58 psi

Sterile filtration capability3
acc. to the bacteria challenge test

Yes

No

No

Yes

Yes

Non-pyrogenic

Yes

Yes

Yes

Yes

Yes

Biocompatible

acc. to ISO 10993-1

Flow Rate for 2 (≥ mL/min)

1 Hold-up volume after air purge
2 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point.
3 According to the bacterial challenge test (BCT) with ≥ 1 × 107 cfu/cm2 Brevundimonas diminuta. All non-sterile Minisart® types listed above can be sterilizedaccording to the sterilization recommendation in this table.

82

Filtration Devices

Minisart® Syringe Filters

CE-Minisart® Syringe Filters for Medical Use

Ordering Information
Minisart® NML ((SF)CA – (Surfactant-free) Cellulose Acetate) Aqueous Filtration∅ in mm | EFA1

Membrane

Housing

Pore Size

Connector Outlet

Color | Printing

Sterile*Qty./Pkg.

Order No.

28 mm

SFCA

MBS

0.2 µmMale Luer Lock

Blue

Yes

50

16534----------K*

28 mm

SFCA

MBS

0.2 µmMale Luer Lock

Blue

Yes#

50

16534--------GUK*

28 mm

SFCA

MBS

0.2 µmMale Luer Lock

Blue

No

500

16534----------Q*

28 mm

SFCA

MBS

0.2 µmMale Luer Slip

Blue

Yes

50

17597----------K *

28 mm

SFCA

MBS

0.2 µmMale Luer Slip

Blue

No

500

17597----------Q*

28 mm

SFCA

MBS

0.45 µm

Male Luer Lock

Yellow

Yes

5016555----------K*

28 mm

SFCA

MBS

0.45 µm

Male Luer Lock

Yellow

Yes#

5016555--------GUK*

28 mm

SFCA

MBS

0.45 µm

Male Luer Lock

Yellow

No

500

16555----------Q*

28 mm

SFCA

MBS

0.45 µm

Male Luer Slip

Yellow

Yes

50

17598----------K*

28 mm

SFCA

MBS

0.45 µm

Male Luer Slip

Yellow

No

500

17598----------Q*

28 mm

CA

MBS

5 µm

Male Luer Lock

Brown

Yes

50

17594----------K*

Pink

Yes

50

17528----------K*

Clear

Yes

50

16596--------HYK*

Clear

No

500

16596--------HYQ*

Clear

No

500

16599--------HYQ*

White, Printed

Yes

50

17575--------ACK*

Minisart® Ophthalsart (SFCA – Cellulose Acetate) Aqueous Filtration
28 mm

SCFA

MBS

0.2 µmMale Luer Slip

Minisart® HY (Hydrophobic PTFE), for Venting and Gas Filtration
26 mm

PTFE

MBS

0.2 µmMale Luer Lock

26 mm

PTFE

MBS

0.2 µmMale Luer Lock

26 mm

PTFE

MBS

0.2 µmMale Luer Locka

Minisart® SRP (Hydrophobic PTFE) Venting & Gas Filtration
25 mm

PTFE

PP

0.2 µmMale Luer Slip

* Article numbers are only available in: EU/EEA and in registered countries.
** Sterilized Minisart® units are individually packaged. If not stated otherwise, Minisarts are sterilized by ethylene oxide.
#-mark indicates sterilization by gamma irradiation
Non-presterilized Minisart® units: (SF)CA can be sterilized by ethylene oxide or gamma irradiation. PTFE can be sterilized by ethylene oxide.
aConnector inlet: Male Luer Slip (all other Minisart® have Female Luer Lock inlet(s)).
1 Diameter of EFA – Effective Filtration Area

For technical product specifications, please see page 81.

Filtration Devices

Claristep® Filtration System

83

Claristep® Filtration System
The Power of Simplicity
Preparing samples by clarification is an essential step prior to nearly all analyticaltechniques, such as high pressure liquid chromatography (HPLC). This filtration
step to eliminate particles is crucial for maintaining the integrity of chromatography columns and for maximizing their operating life time.
In addition, as the sensitivity of automated analytical instruments continues toimprove, they increasingly require less volume to operate in order to maximize
throughput. Therefore, fast clarification of small volumes that does not add
leachables or extractables to the original sample is indispensable for achieving
the best analytical results.
The Claristep® Station consists of a base,a lid and an exchangeable tray for easy andaccurate positioning of sample vials andClaristep® Filter units.
The patent-pending design fea­tures uniquegrooves in the station’s lid and matching guideridges on Claristep® Filter units to enableintuitively correct alignment and convenient
handling of the system.

The grooves automatically guide the filter unit
caps into the correct positions for simultaneousand accurate cap closure.

To meet these requirements, Sartorius has developed a new, easy-to-use andstraightforward filtration setup. The manually operated Claristep® Filtration
System consisting of a station and filter units offers a novel way for clarifying yoursamples prior to analysis.

----	Up to 8 samples are processed simultaneously	No syringe required	No need for a vacuum source or a power supply	For low sample volumes ranging from 60 µL to 600 µL
	Hold-up volume < 30 µL

Claristep® Filter units are made of the purest materials. Another major benefit is that the contact
time of the samples with the filters and the caps is extremely short, ensuring optimal, contamination-free results. Filtered liquids are collected in any 12 × 32 mm outer diameter vials of your choicebased on the analytical method to be performed.

84

Filtration Devices

Claristep® Filtration System

Sample Preparation for Analytics
Use the Most Ergonomic Clarification Solution
Filter 8 samples simultaneously – without needing any power supply or a vacuum | pressure source.
Simply place the filters on your vials, gently close the station and press on the station lid to filter – that’s it!

1. Close the station lid. The
grooves align the caps automatically, securely sealing every single Claristep®
Filter unit for the mostconvenient processing.

2. Apply slight uniform pressure with your hand to
start sample clarification.
You will feel a certain
resistance while liquid ispressed through eachmembrane.

3. Press down on the station
lid so that the left andright corners touch thebase plate. Hold the lid
in place for 3 seconds toensure all sample liquid isfiltered through.

Claristep® Filter units
press liquid through eachmembrane by an air pocketthat forms over each filter
unit when the station lid isclosed. This air pocket isreleased when you stop
holding down the lid – youwill feel it in your fingertips!

Before clarification, the samples are pipetted in the filter reservoire.

After clarification, the filtrates are collected in sample vials.

Filtration Devices

Claristep® Filtration System

85

Reliable Removal of Particles
Filter Samples Without Adding Extractables and Leachables
Claristep® Filter units with RC membranes are optimized for solvents and aqueous solutions. They provide maximum chemical compatibility and exceptionally
low non-specific binding of analytes.

HPLC Certification

mAU
70
60

Filtered with Claristep® RC 0.2 µm
Filtered with Claristep® RC 0.45 µm
Unfiltered Methanol

Methanol

50
40
30
20
10
0
-10

0

10

20

30

40

50

60 minmAU
70
60

Acetonitrile

50

Filtered with Claristep® RC 0.2 µm
Filtered with Claristep® RC 0.45 µm
Unfiltered Acetonitrile

40
30
20
10
0
-10

0

10

20

30

40

50

60 minmAU
70
60Filtered with Claristep® RC 0.2 µm
Filtered with Claristep® RC 0.45 µm
Unfiltered Water

Water

50
40
30
20
10
0
-10

0

10

20

30

40

50

60 minHPLC Procedure
Column: C18: 5 µm × 250 mm × 4.0 mm, Flow Rate: 1 mL/min, Wavelength: 220 nm
Injection Volume: 20 µL, Analysis Time: 65 min, Temperature: 40 °C,
Mobile Phases: A) Acetonitrile | B) Water, Gradient: Hold 60 % A for 10 min, 60 %to 100 % A in 20 min, 100 % A for 30 min

86

Filtration Devices

Claristep® Filtration System

Sample Preparation Techniques
Choose the Best Solution for Your Needs
Do you process dozens of samples each day? A syringeless solution will help
you reduce time, effort and waste – and minimize hand stress. If you need toanalyze only a few samples a day, you will benefit from our proven combinationof a syringe and syringe filter. The choice is all yours!

Claristep®
Filtration Systemor

Syringe Filters
for processing one sample at a time

8 × fold

Analytical Sample Volumes Run Small
Get the Particle-free Volume You Really NeedIf you need to fill only 12 × 32 mm vials, a syringeless solution will help you savetime and reduce sample loss!

Syringeless Solution

orDifferent Diameters
for different sample volumes

1 – 200 mL
0.06 – 0.6 mL

1 – 100 mL

0.5 – 15 mL

0.05 – 1 mLFiltration Devices

Claristep® Filtration System

87

Ordering Information
Claristep® Filters∅ mm | EFD1 Membrane

Housing

Pore Size

SterileQty | Pk

Order No.

9.7 mmRC

PP

0.2 µmNo

96

17C07FT---96

9.7 mmRC

PP

0.2 µmNo

480

17C07FT--480

9.7 mm

RC

PP

0.45 µm

No96

17C06FT---96

9.7 mm

RC

PP

0.45 µm

No

480

17C06FT--480

Qty./Pkg.

Prod. No.

Claristep Station complete

1

17C--M8

Claristep Single Tray

1

17C--S1

1 Effective Filtration Diameter
RC = Regenerated Cellulose

Claristep® Filters are availabe
in a choice of two pore sizes
Claristep® System
Name
®
®

The Tray can be removed and exchanged

Additional Components Needed
The free choice of 12 × 32 mm sample vials and lids is enabeling you to chose theright vial for your particular sample and application, e.g. for light sensitive substances you can use brown glass. For small sample volumes you can use vessels
with inlays. You can use glass or plastic, screw caps and | or slid lids – whatever
you prefer.

12 × 32 mm sample vials

88Filtration Devices

Sartolab® P20 Pressure Filter Units

89

Sartolab® P20 Pressure Filter Units
Compact Design for the Filtration of Large Volumes

Sartolab® P20 devices are ready-to-use pressure filter units for the clarificationand sterile filtration of media and aqueous solutions in batches from 100 mL
to 10 L. Sartolab® P20 Plus with an incorporated prefilter is recommended fordifficult-to-filter solutions like, for example, media that contains serum.
Membrane of Choice
Polyethersulfone (PES) is the membrane of choice for the Sartolab® P20
pressure filter units, as it combines very low protein binding properties with thehighest flow rates. The Sartolab® P20 pressure filter unit is available either with
0.2 μm or 0.45 μm PES membranes, with or without a prefilter made of highpurity quartz microfibers. An additional version containing quartz microfiber onlyis also available for clarification purposes.
Compact Design
Sartolab® P20 pressure filter units have been designed to filter batches from
100 mL to 10 L, either using a syringe or in-line with a peristaltic pump, or apressure vessel.Sartolab® P20 pressure filter units are available in different
configurations, with or without PTFE automatic venting, with or without a fillingbell (including cover) on the outlet and with a combination of different inlet andoutlet connectors to meet the needs of most applications.

---Benefits
	Highest flow rates with a large surface of filtration (20 cm²)
No loss of protein with a low binding membrane
Low dead volume due to an optimized membrane support
Versions available with a prefilter for high particle load solutions

90

Filtration Devices

Sartolab® P20 Pressure Filter Units

Specifications
Different Filter Materials

0.2 µm polyethersulfone
0.45 µm polyethersulfone
High purity binder-free quartz microfibers

Housing Material

Transparent polycarbonate

Filter Diameter

61 mm

Filtration Area

20 cm²

Holdup Volume

Sartolab® P20: 1 mL
Sartolab® P20 Plus: 1.2 mL
Sartolab® P20 Prefilter: 1 mL

Filtration Range

Sartolab® P20: 100 mL to 5 L
Sartolab® P20 Plus: 100 mL to 10 L
Sartolab® P20 Prefilter: 100 mL to 10 L

Recommended Max. Inlet Pressure

4 bar

pH Range

1 – 10

Housing Burst Pressure> 5 bar

Autoclavable

121°C

Ordering Information
Sartolab® P20
Order Number

Filter Material

Inlet

OutletPTFE
Venting

Filling
Bell

Sterilization(EO)

Qty./Pack

18064----------D

0.2 µm PES

Female Luer-LockMale Luer-Lock

yes

no

yes

10

18075----------D

0.2 µm PES

Female Luer-Lock

Hose barb

no

no

yes

10

18075--------UPN

0.2 µm PES

Female Luer-Lock

Hose barb

no

no

no

100

18089----------D

0.2 µm PES

Hose barb

Hose barb

yes

yes

yes

10

18090----------D

0.2 µm PES

Female Luer-LockMale Luer-Lock

no

no

yes

10

Order Number

Filter Material

Inlet

OutletPTFE
Venting

Filling
Bell

Sterilization(EO)

Qty./Pack

18068----------D

Quartz microfibers& 0.2 µm PES

Female Luer-Lock

Hose barb

yes

yes

yes

10

18076----------N

Quartz microfibers& 0.45 µm PES

Hose barb

Hose barb

no

no

no

100

18091----------D

Quartz microfibers& 0.2 µm PES

Hose barb

Hose barb

yes

yes

yes

10

18092----------D

Quartz microfibers& 0.2 µm PES

Female Luer-LockMale Luer-Lock

no

no

yes

10

Order Number

Filter Material

Inlet

OutletPTFE
Venting

Filling
Bell

Sterilization(EO)

Qty./Pack

18072----------D

Quartz microfibers

Female Luer-Lock

Hose barb

no

no

no

10

Sartolab® P20 Plus

Sartolab® P20 Prefilter

Filtration Devices

Sartolab® RF 50

91

Sartolab® RF 50

Vacuum filtration unit for volumes of up to 50 mL
Sartolab® RF 50 vacuum filtration units are single-use units, designed for thefiltration of sample volumes of up to 50 mL. They are available either with a
0.22 or a 0.45 µm polyethersulfone membrane which have been developed andmanufactured by Sartorius at its own facilities. Both membranes provide fastest
flow rates due to their asymmetric structure and ensure lowest protein binding
as well as low extractables. The 0.22 µm version is ideal for sterile filtration ofcell cultures, buffers and aqueous solutions; the 0.45 µm for clarification.
Sartolab® RF 50 are available either individually packed and sterile or in bulk
non-sterile.
Sartolab® RF 50 vacuum filtration unit is composed of a funnel, with dust
cover, a 50 mL conical tube with graduation and writing field as well as a tubing
connector for vacuum connection (sterile versions only) and a screw cap to store
your filtrate in the conical tube. The design of the yellow adapter connecting thefunnel to the conical tube ensures a vacuum-tight seal and enables the filtration
unit to be used on the Sartolab® MultiStation for filtration of up to 6 samples inparallel with one vacuum source.
Sartolab® RF 50 can also be used alone when connecting the tubing connector
delivered with each unit to your vacuum source (sterile versions only). The tubing
connector and the screw cap for the conical tube are individually wrapped tomaintain sterility until needed.

----

User Benefits	Highest flow rates with an asymmetric membrane and a large surface area	No loss of protein with a very low protein binding membrane	Low dead volume thanks to an optimized membrane support
	Minimized risks of contamination with a complete ready-to-use unit
(no further transfer of liquid for storage necessary)	Designed as standalone system or for a parallel filtration of up to 6 samples
with Sartolab® MultiStation

92

Filtration Devices
Chapter
Sartolab® RF 50

Filtration of 50 mL cell media + 10 % FBS in a 50 mL vacuum filtration unit (0.22 µm)
7s

Sartolab® RF 50

17 s

Brand D

22 s

Brand C

14 s

Brand B

30 s

Brand A

0

5

10

15

20

25

30

Materials
Funnel with dust cover

Polystyrene (PS)Membrane filter

0.22 μm polyethersulfone (order no. 180E01)
0.45 µm polyethersulfone (order no. 180F01)

Funnel adapter

High Density Polyethylene (HDPE)

Tubing connector for vacuum connection

High Density Polyethylene (HDPE)

Conical tube

Polypropylene

Conical tube cap

High Density Polyethylene (HDPE)

Specifications
Membrane ∅58.5 mm

Effective filtration area

21 cm²

Hold-up volume

1.2 mL

Filtration capacity

50 mL

Size of the 50 mL conical tube

Height: 115.5 mm, External/internal diameter:29.5 mm/27.48 mm

Autoclavable

No

Sterilization method

E-Beam (beta) irradiation

Storage temperature of the conical tube

4 °C to 30 °C (short-term: –80 °C to max. 100 °C)

Packaging

Single-packaged, sterile

Operating pressure–350 to –700 mbar

Ordering Information
Description

Quantity

Order No.

Sartolab RF 50, 0.22 µm, PES

24 units

180E01---------2

Sartolab RF 50, 0.45 µm, PES

24 units

180F01---------2

Sartolab RF 50, 0.22 μm, PES, non-sterile

96 units

180E01––––––––E8

Sartolab RF 50, 0.45 μm, PES, non-sterile

96 units

180F01––––––––E8

®
®
®
®

35

Filtration Devices

Sartolab® RF | BT

93

Sartolab® RF | BT
Vacuum Filtration Units

Sartolab® RF | BT vacuum filtration units are convenient filtration units designed
for research purposes and, therefore, for the filtration of small volumes from> 50 mL to 1 L. Sartolab® RF as a complete system includes a receiver flask to
the filtration funnel. Sartolab® BT is a bottle top filter (filtration funnel) withouta receiver flask, enabling customers to use their own receiver flasks and/or to
expand the filtration capacity, depending on the particle load of the filteredliquid, by filling more than one receiver flask.
Membrane of Choice
Polyethersulfone is the membrane of choice for the Sartolab® RF | BT vacuum
filtration units as it combines very low protein binding properties and highest
flow rates. The 0.22 μm polyethersulfone membrane belongs to the best
asymmetric membrane in the market.
The Sartolab® RF | BT vacuum filtration units are available in 3 different pore sizes
to meet most of the applications: 0.1 μm for mycoplasma removal
 0.22 μm for the sterile filtration of cell culture, media, buffers, and reagents
 0.45 μm for the clarification of aqueous and viscous solutions
Ergonomic Design
Sartolab® RF | BT vacuum filtration units have been designed to maximallyfacilitate the user’s daily work.
 Ergonomic design of the 150 mL to 1 L bottles for easy grip with one hand anddesignated writing field on the back for clear labeling of samples
 Engraved graduations on the funnels and the bottles ensure accuracy andhighest readability
 The footprint of the bottles gives good stability for the unit during filtration
 No extra tightening of the funnel before filtration required (vacuum-tightsealed) The funnels and bottles are stackable to save space not only in the refrigerator
but also in the bin The design of the yellow adapter connecting the funnel to the bottles enables
the filtration unit to be used on the Sartolab® Multistation for filtration of up to
6 samples in parallel with one vacuum source The ergonomic soft blister packaging is not only easy to open but its design
facilitates the transportation of several units with one handState-of-the-Art Production
 Sartolab® RF | BT vacuum filtration units are manufactured in an ISO 13485
certified plant and ISO
 Class 8 cleanroom to assure the highest level of purity
 All fluid path materials used in the production of the Sartolab® RF | BT vacuum
filtration units are medical graded for highest quality, without any animal origin
 All products are sold sterilized and guaranteed endotoxin-free All fluid path component materials meet the requirements for United States
Pharmacopeia (USP) Class VI Biological Test for Plastics, latest volume
 The fluid path component materials are determined to be non-cytotoxic in
accordance to ISO 10993

94

Filtration Devices

Sartolab® RF | BT

Best Engineering Optimized membrane support for lowest hold-up volumes and for the
reducing of foam formation and thus a denaturation of proteins Delivered with a vacuum tube connector for stand-alone filtration
 For the Sartolab® RF versions, the screw caps of the bottles are delivered extra
packed to maintain sterility up to the end of filtration
 The 45 mm neck thread of the Sartolab® units ensures a vacuum-tight seal tobottles with this standard thread The risk of contamination is minimized with the complete ready-to-use unit
Sartolab® RF versions

Technical Specifications
Material
Membrane filter

0.1 µm polyethersulfone (Cat. No. 180D*)
0.22 µmpolyethersulfone (Cat. No. 180E*)
0.45 µm polyethersulfone (Cat. No. 180F*)

Funnel, lid, and bottle

Polystyrene (PS)

Tubing connector, funnel adapter, andscrew cap

High Density Polyethylene (HDPE)

Packaging

PET|PE and PE|PA multilayer films

Specifications
Membrane diameter

80 mm for 150 mL and 250 mL volumes
100 mm for 500 mL and 1,000 mL volumes

Effective filtration area

43 cm² for 150 mL and 250 mL volumes
69 cm² for 500 mL and 1,000 mL volumes

Bottle neck size

45 mm

Autoclavable

No

Sterilization method

E-Beam (beta) irradiation (SAL 10-6)

Transportation and storage
temperatures-20° C to + 60° C

Operational temperatures

0° C to 70° C

Packaging

Single-packaged, soft blister, sterile

Operating pressure-350 to -750 mbar

Hold up volumes (for water)

2.7 mL for 150 mL and 250 mL versions
4.1 mL for 500 mL and 1,000 mL versions

Comparison of Filtration Times [s] for 500 mL Cell Media + 10% FBS in Six 0.22 μm 500 mL Vacuum Filtration Units

68

41

37

32
24

Brand A

Brand B

Sartorius

Brand C

Brand D28

Brand E

Filtration Devices

Sartolab® RF | BT

95

Ordering Information
Sartolab® RF

The Sartolab® RF vacuum filtration unit iscomprised of: A graduated funnel with apolyethersulfone (PES) membrane, avacuum adapter and a lid A bottle, with graduation and writingfield A tube connector for vacuum
connection (for stand-alone filtration)
 A screw cap for storage of the filtrate(individually wrapped to maintainsterility)

Order Number

Description

Membrane Type

Pore
Size
(µm)

180E02–––––––––E

Sartolab® RF 150

Asymmetric PES

0.22

150

150

12

180F02–––––––––E®

Sartolab RF 150

PES

0.45

150

150

12

180D03–––––––––E

Sartolab RF 250

PES

0.1

250

250

12

180E03–––––––––E

Sartolab RF 250

Asymmetric PES

0.22

250

250

12

180F03–––––––––E

Sartolab RF 250

PES

0.45

250

250

12

180E04–––––––––E

Sartolab® RF 500

Asymmetric PES

0.22

500

500

12

180F04–––––––––E

Sartolab® RF 500

PES

0.45

500

500

12

180D05–––––––––E

Sartolab® RF 1,000

PES

0.1

1,000

1,000

12

180E05–––––––––E

Sartolab® RF 1,000

Asymmetric PES

0.22

1,000

1,000

12

180F05–––––––––E

Sartolab RF 1,000

PES

0.45

1,000

1,000

12

Order Number

Description

Membrane Type

Pore Size(µm)

FunnelVolume
(mL)

Pkg.
Unit

180E12–––––––––E

Sartolab® BT 150

Asymmetric PES

0.22

150

12

180E13–––––––––E®

Sartolab BT 250

Asymmetric PES

0.22

250

12

180E14–––––––––E

Sartolab® BT 500

Asymmetric PES

0.22

500

12

180E15–––––––––E

Sartolab® BT 1,000

Asymmetric PES

0.22

1,000

12

180F15–––––––––E

Sartolab® BT 1,000

PES

0.45

1,000

12

®
®
®

®

FunnelVolume(mL)

BottleVolume
(mL)

Pkg.
Unit

Sartolab® BT

The Sartolab BT bottle top filter iscomprised of: A graduated funnel with apolyethersulfone (PES) membrane,
a vacuum adapter, and a lid A tubing connector for vacuum
connection (for use as stand-alone)®

Accessories and ConsumablesMultistation
For hands-free parallel filtration of up to six samples
Order Number

Description

Pkg. Unit

SDLC01

Sartolab Multistation

1®

Sartolab® Bottle
Delivered sterile, for filtration and storage
Order Number

Description

Volume (mL)

Pkg.Unit180-22–––––––––E

Sartolab bottle 150 mL

150

12

180-23–––––––––E

Sartolab bottle 250 mL

250

12

180-24–––––––––E

Sartolab bottle 500 mL

500

12

180-25–––––––––E

Sartolab bottle 1,000 mL

1,000

12

®
®
®
®

Binder-Free Glass Microfiber Prefilters
High purity prefilters to prevent the clogging of the membrane when filtering viscous or particulateloaded solutions
Order Number

Description

Filter Diameter (mm)Pkg. Unit

FT-3-1101-080

Binder-free glass microfiber filter discs,
grade MGA, for 150 and 250 mL funnels

80

100

FT-3-1101-100

Binder-free glass microfiber filter discs,
100
grade MGA, for 500 and 1,000 mL funnels

100

96

Filtration Devices

Sartolab® Multistation

Sartolab® Multistation

For hands-free parallel filtration of up to 6 samples
Sartolab® MultiStation is a stand specially designed to hold 1 to 6 vacuum filtration units, allowing simultaneous filtration of up to 6 samples.
The MultiStation is permanently connected to your vacuum source. Easily installyour vacuum filtration units in the MultiStation for quick and easy filtration ofsamples without the need for installation of extra connectors and time-consuming stabilization.
Sartolab® MultiStation works with all Sartolab® RF | BT vacuum filtration units;
the funnel adapter of these units is designed to fit perfectly in the bracket of
the MultiStation. With one click, connect the filtration unit to the device, assuring perfect filter stability. With a second click, engage the vacuum automaticallyand begin filtering.
Easily manipulate your samples with the rotating, multi-directional head, andeasily keep track of your samples during filtration with the numbered brackets---

User Benefits	Single vacuum source enables simultaneous filtration of up to 6 samples
	Time-saving (no installation time for each filter unit before use)	Hands-free filtration

Filtration Devices

Sartolab® Multistation

97

The MultiStation advantage: Using the Sartolab® MultiStation to filter
6 x 50 mL samples saves handling time compared to standalone filtration

Filtration of 50 mL cell media + 10 % FBS in a 50 mL vacuum filtration unit (0.22 µm)
450
400

60

350
5

15

4

1

300

2

250
200

360

150

300

100

6x

50
0
Standalone(Filtration of 6 Samples in succession)Multistation(Filtration of 6 Samples in parallel)

Preparation (before and after the filtration) in s
Filtration time in sSpecifications
Material (visible parts)

ABS | Aluminum | Stainless steel

Dimensions (Length × Width × Height)

307 × 348 × 281 mm

Weight4.6 kgTubing connector

Designed for tubing with an inner diameter
between 4 and 10 mm and with a wall thickness ofminimum 3 mm

Ordering Information
Description

Quantity

Order No.

Sartolab MultiStation

1 unitSDLC01®

98

Filtration Devices

Sartoclear Dynamics® Lab P15

Sartoclear Dynamics® Lab P15

Clarification and Sterile Filtration of up to 15 mL Mammalian Cell Culture
in One Step
Sartoclear Dynamics® Lab P15 is a kit for single step harvesting of 15 mL animal
cell cultures with even high cell densities. With this kit, the clarification andsterile filtration of mammalian cell culture is performed in a single pressure filtration step. Inspired by the plasma industry, Sartoclear Dynamics® is based on theprinciples of body feed filtration.
This ready to use kit combines a 20 mL syringe pre-filled with a 0.5 g filter aid andan integrated filter* for sterile filtration. The filter aid facilitates filtration throughthe sterile filter while allowing complete protein recovery.
A convenient filling tube can be connected to the syringe, for the easy recovery
of samples from 50 mL Falcon tubes or ambr 15 bioreactors.
As a result, this method replaces centrifugation and subsequent sterile filtration
steps, leading to clarified and sterilized cell culture harvest in minutes. Your cell
culture harvest will be available for following sample concentration by ultrafiltration and downstream analytics in no time.

---

Sartoclear® Dynamics Lab P15 Features:	Single step mammalian cell culture harvest	Designed for cell densities up to 20 × 106 cells and even more	Fast and effortless filtration
	Optimized for cell culture harvest from ambr® 15 bioreactors* The sterile filter included in the kit contains a 0.2 µm polyethersulfone membrane and a pre­filter made of 100 % high-purity quartz:
The choice of these materials, along with the larger surface area of the filter, enables higher flow rates.

Quick and Easy Filtration

1. Fill the syringe with cell culture broth.

2. S hake the syringe to mix the broth withthe filter aid.

3. C
 onnect the sterile filter to the syringe
and filter.

Typical Results
Cell Type

Cell Density

Viability

Mab concentration
before filtration

Mab concentration
after filtration

Recovery Rate

CHO DG44

16 × 106 cells/mL

78 %6.02 g/L

5.77 g/L

96 %CHO DG44

38 × 105 cells/mL

48 %0.43 g/L

0.43 g/L

100 %Filtration Devices

Sartoclear Dynamics® Lab P15

99

Specifications
DE Syringe
Syringe material

Syringe barrel and plunger rod: polypropylene;
stopper: latex-free elastomer

Syringe Cap

Polyamide

Filling tube material

Polypropylene

Filter aid

0.5 g highly pure diatomaceous earth(Celpure® C300 – pharmaceutical-grade*)

Sterile Filter
Housing material

Polycarbonate

Prefilter material

100 % high-purity quartz, binder-freeFilter material

0.2 µm polyethersulfone

Filter ∅61 mm

Filtration area

20 cm²Connector inlet

Female Luer-Lock

Connector outlet

Male Luer-Lock

Hold-up volume

Approx. 2.5 mL

Housing burst pressure> 5 bar | 72.5 psi

Packaging

Individually packed

Sterilization

EO sterilization

Ordering Information
Product Name

Number of units per box

Sartoclear Dynamics®
Lab P15 6 × 20 mL syringes, pre-filled SDLP--0015-----C
with 0.5 g DE, including caps
and filling tubes
6 × 0.2 µm PES sterile filters* Celpure® is a trademark of Advanced Minerals

Order No.

100

Filtration Devices

Sartoclear Dynamics® Lab V

Sartoclear Dynamics® Lab V

Clarification and Sterile Filtration of 50 mL up to 1 L Mammalian Cell Culture
in One Step
Sartoclear Dynamics® Lab V kits enable clarification and sterile filtration to be
performed in a single step. These kits simplify the cell harvesting process by fully
eliminating the centrifugation step otherwise needed for clarification. As a result,they enable cell cultures to be efficiently clarified and sterilized in minutes –quickly and easily.
Sartoclear Dynamics® Lab kits have been designed and optimized for harvesting
mammalian cell cultures, such as CHO, HEK, hybridomas and many others, withcell densities of up to 20 × 106 cells/mL.
Each kit provides filter aid pouches for clarification and Sartolab® RF vacuum
filtration units for sterile filtration. The filter aid used in Sartoclear Dynamics® Lab
products is made of highly-pure diatomaceous earth (DE) that is insoluble andinert. It is packed in ready-to-use pouches in pre-wetted condition to preventthe release of dust particles. The DE pouches are gamma-irradiated to rule out
any contamination.

---

Sartoclear® Dynamics Lab V Features:
Single step mammalian cell culture harvest
Designed for cell densities up to 20 × 106 cells
Fast and effortless filtration

Typical Results
Cell Type

Cell Density

Viability

Mab concentration
before filtration

Mab concentration
after filtration

Recovery Rate

Turbidity

CHO

14.46 ×106 cells/mL

85.2 %

5.2 g/L

5.15 g/L

99 %18 NTU

HEK

8 ×106 cells/mL

70 %0.035 g/L

0.034 g/L

97 %

8 NTU

Filtration Devices

Sartoclear Dynamics® Lab V

101

Specifications
Clarification
Pouches of Filter Aid
Diatomaceous Earth (DE)

1 g, 5 g or 10 g highly pure diatomaceous earth,(Celpure® C300 – pharmaceutical grade)*, mixed with waterin a ratio of 1 DE: 1.25 ultrapure water

Packaging | Sterilization

Dust-free, gamma irradiated pouches

Filtration
Vacuum filtration units with receiver flasks (Sartolab® RF 150 – 1000)
Funnel, dust cover,receiver bottles

Polystyrene (PS)

Filter adapter,tubing connector, cap

High Density Polyethylene (HDPE)

Filter material

0.22 µm polyethersulfone

Packaging | Sterilization

Single-packaged, sterile

Filtration
Vacuum filtration units with conical tube (Sartolab® RF 50)
Funnel, dust cover

Polystyrene (PS)

Filter adapter,tubing connector, cap

High Density Polyethylene (HDPE)

Conical tube

Polypropylene

Filter material

0.22 µm polyethersulfone (order no. 180E01)
0.45 µm polyethersulfone (order no. 180F01)

Packaging

Single-packaged, sterile* Celpure® is a trademark of Advanced Minerals

Each Sartoclear Dynamics® Lab V kit is comprised of pouches of filter aid andSartolab® RF vacuum filtration units that match your needs. Find the right kit
in just two easy steps:
1. Determine the volume range of your sample to be filtered.
2. Then reference it to the cell density of your cell culture.
VolumeCell density*< 5 million cells/mL

5 – 10 million cells/mL

10 – 20 million cells/mL≤ 50

SDLV-0050-01E0-2

SDLV-0050-02E0-2> 50 – 150 mL

SDLV-0150-02E0-E

SDLV-0150-05E0-2

150 – 250 mL

SDLV-0250-05E0-2

SDLV-0250-10E0-2

250 – 500 mL

SDLV-0500-05E0-2

SDLV-0500-10E0-2

SDLV-0500-20E0-E

500 – 1,000 mL

SDLV-1000-10E0-2

SDLV-1000-20E0-E

SDLV-1000-40E0-E* Tested with CHO cell lines with a cell viability of approx. 85 %

102

Filtration Devices

Sartoclear Dynamics® Lab V

Ordering Information
Sartoclear Dynamics® Lab V50 Kits – 0.22 µm PES
Sartoclear Dynamics Lab V, 50 mL, 1 g
Description

Qty.
of Units

Order No.

Filtration of up to 50 mL with 1 g of DE per unit
Contents: 1 × 180E01----------2(24 × Sartolab® RF 50; 0.22 µm; PES)
1 × SDLKG-01.0------2(24 × pouches of filter aid, 1 g)

24

SDLV-0050-01E0-2

24

SDLV-0050-02E0-2

24

SDLV-0050-01F0-2

24

SDLV-0050-02F0-2

12

SDLV-0150-02E0-E

24

SDLV-0150-05E0-2

Sartoclear Dynamics Lab V, 50 mL, 2 g
Filtration of up to 50 mL with 2 g of DE per unit
Contents: 1 × 180E01----------2(24 × Sartolab® RF 50, 0.22 µm PES)
2 × SDLKG-01.0------2(48 × pouches of filter aid, 1 g)

Sartoclear Dynamics® Lab V50 Kits – 0.45 µm PES
Sartoclear Dynamics Lab V, 50 mL, 1 g
Filtration of up to 50 mL with 1 g of DE per unit
Contents: 1 × 180F01----------2(24 × Sartolab® RF 50, 0.45 µm PES )1 × SDLKG-01.0------2(24 × pouches of filter aid, 1 g)
Sartoclear Dynamics Lab V, 50 mL, 2 g
Filtration of up to 50 mL with 2 g of DE per unit
Contents: 1 × 180F01----------E(24 × Sartolab® RF 50, 0.45 µm PES)
2 × SDLKG-01.0------2(48 × pouches of filter aid, 1 g)

Sartoclear Dynamics® Lab V150 Kits
Sartoclear Dynamics® Lab V, 150 mL, 2 g
Filtration of up to 150 mL with 2 g of DE per unit
Contents: 1 × 180E02---------E(12 × Sartolab® RF 150, 0.22 µm PES)
1 × SDLKG-01.0------2(24 × pouches of filter aid, 1 g)
Sartoclear Dynamics® Lab V, 150 mL, 5 g
Filtration of up to 150 mL with 5 g of DE per unit
Contents: 2 × 180E02---------E(24 × Sartolab® RF 150, 0.22 µm PES)
1 × SDLKG-05.0------2(24 × pouches of filter aid, 5 g)Filtration Devices

Sartoclear Dynamics® Lab V

103

Sartoclear Dynamics® Lab V250 Kits
Sartoclear Dynamics® Lab V, 250 mL, 5 g
Description

Qty.
of Units

Order No.

Filtration of up to 250 mL with 5 g of DE per unit
Contents: 2 × 180E03---------E(24 × Sartolab® RF 250, 0.22 µm PES)
1 × SDLKG-05.0------2(24 × pouches of filter aid, 5 g)

24

SDLV-0250-05E0-2

24

SDLV-0250-10E0-2

24

SDLV-0500-05E0-2

24

SDLV-0500-10E0-2

12SDLV-0500-20E0-E

24

SDLV-1000-10E0-2

24

SDLV-1000-20E0-E

12SDLV-1000-40E0-E

Sartoclear Dynamics® Lab V, 250 mL, 10 g
Filtration of up to 250 mL with 10 g of DE per unit
Contents: 2 × 180E03---------E(24 × Sartolab® RF 250, 0.22 µm PES)
1 × SDLKG-10.0------2(24 × pouches of filter aid, 10 g)

Sartoclear Dynamics® Lab V500 Kits
Sartoclear Dynamics® Lab V, 500 mL, 5 g
Filtration of up to 500 mL with 5 g DE per unit
Contents: 2 × 180E04---------E(24 × Sartolab® RF 500, 0.22 µm PES)
1 × SDLKG-05.0------2(24 × pouches of filter aid, 5 g)
Sartoclear Dynamics® Lab V, 500 mL, 10 g
Filtration of up to 500 mL with 10 g DE per unit
Contents: 2 × 180E04---------E(24 × Sartolab® RF 500, 0.22 µm PES)
1 × SDLKG-010.0------2(24 × pouches of filter aid, 10 g)
Sartoclear Dynamics® Lab V, 500 mL, 20 g
Filtration of up to 500 mL with 20 g DE per unit
Contents: 1 × 180E04---------E(12 × Sartolab® RF 500, 0.22 µm PES)
1 × SDLKG-010.0------2(24 × pouches of filter aid, 10 g)

Sartoclear Dynamics® Lab V1000 Kits
Sartoclear Dynamics® Lab V, 1,000 mL, 10 g
Filtration of up to 1 L with 10 g of DE per unit
Contents: 2 × 180E05---------E(24 × Sartolab® RF 1000, 0.22 µm PES)
1 × SDLKG-10.0------2(24 × pouches of filter aid, 10 g)
Sartoclear Dynamics® Lab V, 1,000 mL, 20 g
Filtration of up to 1 L with 20 g of DE per unit
Contents: 1 × 180C3----------E(12 × Sartolab® RF 1000, 0.22 µm PES)
1 × SDLKG-10.0------2(24 × pouches of filter aid, 10 g)
Sartoclear Dynamics® Lab V, 1,000 mL, 40 g
Filtration of up to 1 L with 40 g of DE per unit
Contents: 1 × 180E05---------E(12 × Sartolab® RF 1000, 0.22 µm PES)
2 × SDLKG-10.0------2(48 × pouches of filter aid, 10 g)

104

Filtration Devices

Chemical Compatibility

Chemical Compatibility

Pre-Filter

GF

Housing Material

MBS

PP

Minisart® RC

Minisart® NY

Minisart® NY Plus

Minisart® SRP

Minisart® PES

PES (SF)CA (SF)CAMinisart® HY
Minisart® Air

Minisart® NML GF

Minisart® NML Plus

Minisart® NML
Ophthalsart

Minisart® HighFlow

Housing PP

Housing MBS

Nylon membranePA

GF depth filter

RC membrane
RC

SFCA membrane

PES (SF)CA PTFEPES membrane
Filter Membrane

Minisart® Types
PTFE membrane

Material

PTFE

RC

PA

PA

PTFE

PES

-

-

GF

GF

-

-

-

GF

-

-

MBS

MBS

MBS

MBS

MBS

PP

PP

PP

PP

PP
++

Sterilization
Ethylene oxide

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

Gamma irradiation

++

++

-1

++

-

++

++

-

++

++

++

++

-1

-

-

-

-

-

Autoclaving 121 °C, 30 min

++

++

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

++

Solvents
Acetone

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

Acetonitrile

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

Benzene

+

+

-

++

++

++

-

++

-

-

-

-

-

++

++

++

-

+

Benzyl alcohol

+

+

++

++

++

++

-

+

-

-

-

-

-

++

++

++

++

+

n-Butyl acetate

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

n-Butanol

++

++

++

++

++

++

+

++

+

+

+

+

+

++

++

++

++

++
+

Cellosolve

+

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

Chloroform

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

Cyclohexane

-

-

++

++

++

++

+

+

-

-

-

-

+

+

+

+

+

-

Cyclohexanone

-

-

++

++

++

++

-

+

-

-

-

-

-

+

+

+

+

-

Diethylacetamide

-

-

-

++

++

++

-

++

-

-

-

-

-

++

++

++

-

-

Diethyl ether

-

+

-

++

++

++

-

++

-

-

-

-

-

++

++

++

-

-

Dimethyl formamide

-

-

++

+

+

++

-

++

-

-

-

-

-

+

+

+

++

-

Dimethylsulfoxide

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

Dioxane

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

++

Ethanol, 98 %

++

++

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

Ethyl acetate

-

-

++

++

++

++

-

+

-

-

-

-

-

+

+

+

+

-

Ethylene glycol

++

+

++

++

++

++

+

++

+

+

+

+

+

++

++

++

++

++

Formamide

++

-

+

+

++

++

++

++

++

-

-

-

+

+

++

++

++

++

Glycerin

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

++

n-Heptane

+

+

+

++

++

++

++

+

+

+

+

+

+

+

+

+

+

+

n-Hexane

+

+

+

++

++

++

++

+

+

+

+

+

+

+

+

+

-

+

Isobutanol

++

+

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

++

Isopropanol

++

++

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

++

Isopropyl acetate

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

Methanol, 98 %

+

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

+

Methyl acetate

-

-

++

++

++

++

-

+

-

-

-

-

-

+

+

+

+

-

Methylene chloride

-

-

-

++

++

++

-

++

-

-

-

-

-

++

++

++

-

-

Methyl ethyl ketone

-

+

++

++

++

++

-

+

-

-

-

-

-

+

+

+

+

-

Methyl isobutyl ketone

-

-

++

++

++

++

-

+

-

-

-

-

-

+

+

+

+

-

Monochlorobenzene

+

+

-

++

++

++

-

+

-

-

-

-

-

+

+

+

-

+

Nitrobenzene

-

-

++

++

+

++

-

+

-

-

-

-

-

+

+

+

+

-

n-Pentane

++

++

-

++

++

++

+

+

+

+

+

+

+

+

+

+

-

+

Perchloroethylene

-

-

-

++

++

++

-

+

-

-

-

-

-

+

+

+

-

-

Petroleum ether

+

++

-

++

++

++

+

++

+

+

+

+

-

++

++

++

-

+

Filtration Devices

Chemical Compatibility

Prefilter

GF

Housing Material

MBS

PP

Minisart® RC

Minisart® NY

Minisart® NY Plus

Minisart® SRP

Minisart® PES

PES (SF)CA (SF)CAMinisart® HY
Minisart® Air

Minisart® NML GF

Minisart® NML Plus

Minisart® NML
Ophthalsart

Minisart® HighFlow

Housing PP

Housing MBS

Nylon membranePA

GF depth filter

RC membrane
RC

SFCA membrane

PES (SF)CA PTFEPES membrane
Filter Membrane

Minisart® Types
PTFE membrane

Material

105

PTFE

RC

PA

PA

PTFE

PES

-

-

GF

GF

-

-

-

GF

-

-

MBS

MBS

MBS

MBS

MBS

PP

PP

PP

PP

PP

Solvents (continued)
Pyridine

-

-

++

++

++

++

-

++

-

-

-

-

-

++

++

++

++

-

Tetrahydrofuran

-

-

-

++

++

++

-

++

-

-

-

-

-

++

++

++

-

-

Toluene

-

+

-

++

++

++

-

+

-

-

-

-

-

+

+

+

-

-

Trichloroethylene

-

+

++

++

++

++

-

+

-

-

-

-

-

+

+

+

+

-

Xylene

-

+

-

++

++

++

-

+

-

-

-

-

-

+

+

+

-

+

Acids
Acetic acid, 25 %

+

+

++

++

-

++

+

++

+

+

+

+

+

++

-

-

++

Acetic acid, 80 %

-

-

++

+

-

++

-

+

-

-

-

-

-

+

-

-

+

-

Hydrofluoric acid, 50 %

+

-

++

+

-

++

-

+

-

-

-

-

-

+

-

-

+

+

Perchloric acid, 25 %

-

-

++

-

-

++

-

+

-

-

-

-

-

-

-

-

+

-

Phosphoric acid, up to 10 %

+

+

++

-

-

++

+

+

+

+

+

+

+

-

-

-

+

+

Phosphoric acid, 86 %

+

+

++

-

-

++

-

+

-

-

-

-

-

-

-

-

+

+

Nitric acid, 30 %

+

-

++

-

-

++

+

+

+

-

-

-

+

-

-

-

+

+

Nitric acid, conc.

-

-

++

-

-

++

-

-

-

-

-

-

-

-

-

-

-

-

Hydrochloric acid, 20 %

++

-

++

-

-

++

+

+

+

-

-

-

+

-

-

-

+

+

Sulfuric acid, 25 %

+

-

++

+

-

++

++

++

+

-

-

-

++

+

-

-

++

+

Sulfuric acid, 98 %

-

-

++

-

-

++

-

-

-

-

-

-

-

-

-

-

-

-

Trichloroacetic acid, 25 %

-

-

++

++

-

++

-

+

-

-

-

-

-

+

-

-

+

-

Ammonia, 1N

++

+

++

+

++

++

+

++

+

+

+

+

+

+

++

++

++

++

Ammonium hydroxide, 25 %

+

+

++

+

++

+

-

+

-

-

-

-

-

+

+

+

+

+

Potassium hydroxide, 32 %

++

-

++

-

+

+

-

++

-

-

-

-

-

-

+

+

++

++

Bases

Sodium hydroxide, 1N

++

-

-

+

++

+

-

++

-

-

-

-

-

+

++

+

-

++

Sodium hydroxide, 32 %

++

-

-

-

+

-

-

+

-

-

-

-

-

-

+

-

-

+

Aqueous solutions
Formaldehyde, 30 %

+

++

++

+

++

++

+

+

+

+

+

+

+

+

+

+

+

+

Sodium hypochlorite, 5 %

++

-

++

-

-

++

+

+

+

-

-

-

+

-

-

-

+

+

Hydrogen peroxide, 35 %

++

-

++

-

-

++

+

++

+

-

-

+

+

-

-

-

++

++

pH range
pH 1 to 14

-

-

++

-

-

++

-

++

pH 1 to 13

++

-

++

-

-

++

-

++

pH 3 to 14

+

-

++

+

++

++

-

++

pH 3 to 12

++

-

++

++

++

++

+

++

pH 4 to 8

++

++

++

++

++

++

++

++

The chemical compatibility guide could be confirmed either by a literature review or by laboratory tests. Please consider that compatibilities can be influenced by various factors.
Therefore, we recommend that you confirm compatibility with the liquid you want to filter by performing a trial filtration run before you start your actual filtration.

Legend++ High compatibility+ Limited compatibility- Not compatible
1 Gamma irradiation feasible for Minisart® Air

107

Basic Filtration
Table of Contents

Filter Papers 
Glass and Quartz Microfiber Filters
Membrane Filters
Blotting | Chromatography Papers & Blotting Membranes
Filtration Equipment
Combisart® Manifolds 

112
117
122
131
133
143

108

Basic Filtration

Introduction

Introduction
Filters are indispensable for your routine work in laboratory
and industrial applications. Sartorius supplies you with abroad range of filters for a myriad of ­filtration tasks and supports you with all your filtration challenges.

----

Our Product Range Covers:
	Filter papers
	Glass and quartz microfiber filters
	Membrane filters
	Blotting & chromatography papers & membranes
	Filtration equipment
Quality Assurance and Quality Control
Sartorius pays particular attention to continuous ­in-process
quality control. Regular checks and exact analyses of the
raw materials and each finished product assure constanthigh quality and product uniformity.
We meet the requirements set forth by the ISO 9001 quality management system and the ISO 14001 ­environmental
management system.

How Do Filter Papers Work?
Filter papers are depth filters. Their efficiency is influenced
by various parameters: the mechanical particulate retention,
adsorption, pH, surface properties, thickness and strengthof the filter paper as well as the shape, density and quan­tity of particles to be retained. The precipitates depositedon the filter form a “cake layer” which – depending on its
density – increasingly affects the progress of an ongoing
filtration and decisively affects the retention capability.
Therefore, it is essential to select the perfect filter paper toensure the best filtration results. This choice depends on thefiltration method as well as on the amount and propertiesof the medium to be filtered, the size of the particulate solids
to be removed and the required degree of clarification.
How Do Membrane Filters Work?
Membrane filters retain particles larger than their poresizes. Smaller particles pass through the membrane or are
captured in the membrane. Such filters are used for the filtration of smaller particles and for critical applications suchas sterility testing. The choice of the right membrane type
depends on the specifications of the solution to be filtered.
The most important parameters for this are adsorption,
chemical compatibility and the particle size to be retained.

109

110

Basic Filtration

Filter Papers

Ash-free Filter Papers

For Quantitative and Gravimetric Analyses
These filter papers are used for quantitative and gravimetric analyses as well as for pressure or vacuum filtration. They are
made out of 100 % cotton linters with an α-cellulose content of > 98 % and are acid-washed to make the papers ashless andachieve high purity.

Typical Values
Grade

Weight(g/m2)

Thickness(mm)

Particle retention(μm)

Filtration(s)

Precipitates

Properties

388

84

0.21

12 – 15

10

Coarse crystalline

Wide-pore, loose structure, fast filtering

389

84

0.19

8 – 1220

Medium-fine crystalline

Medium- to wide-pore, medium fast filtering

392

84

0.17

5–8

50

Fine crystalline

Medium dense, medium fast filtering

390

84

0.16

3–5

100

Fine crystalline

Narrow-pore, dense, slow filtering

391

84

0.15

2–3

180

Very fine crystalline

Fine-pore, dense, very slow filtering

393

100

0.18

1–2

300

Very fine crystalline

Very fine-pore, very dense, very slow filtering

Ordering Information			Filter Discs, 100 pieces∅ in mm

Grade 388

Grade 389

Grade 390

Grade 391

Grade 392

Grade 393

55

FT-3-101-055

FT-3-102-055

FT-3-103-055

FT-3-104-055

FT-3-105-055

FT-3-127-055

70

FT-3-101-070

FT-3-102-070

FT-3-103-070

FT-3-104-070

FT-3-105-070

FT-3-127-070

90

FT-3-101-090

FT-3-102-090

FT-3-103-090

FT-3-104-090

FT-3-105-090

FT-3-127-090

110

FT-3-101-110

FT-3-102-110

FT-3-103-110

FT-3-104-110

FT-3-105-110

FT-3-127-110

125

FT-3-101-125

FT-3-102-125

FT-3-103-125

FT-3-104-125

FT-3-105-125

FT-3-127-125

150

FT-3-101-150

FT-3-102-150

FT-3-103-150

FT-3-104-150

FT-3-105-150

FT-3-127-150

185

FT-3-101-185

FT-3-102-185

FT-3-103-185

FT-3-104-185

FT-3-105-185

FT-3-127-185

240

FT-3-101-240

FT-3-102-240

FT-3-103-240

FT-3-104-240

FT-3-105-240

FT-3-127-240

			Folded Filters, 100 pieces∅ in mm

Grade 388

Grade 389

Grade 390

Grade 391

Grade 392

110

FT-4-101-110

FT-4-102-110

FT-4-103-110

FT-4-104-110

FT-4-105-110

125

FT-4-101-125

FT-4-102-125

FT-4-103-125

FT-4-104-125

FT-4-105-125

150

FT-4-101-150

FT-4-102-150

FT-4-103-150

FT-4-104-150

FT-4-105-150

185

FT-4-101-185

FT-4-102-185

FT-4-103-185

FT-4-104-185

FT-4-105-185

240

FT-4-101-240

FT-4-102-240

			

FT-4-104-240

Sheets in 580 × 580 mm, 100 pieces

Grade 388

Grade 389

Grade 390

Grade 391

Grade 392

Grade 393

FT-2-101-580580

FT-2-102-580580

FT-2-103-580580

FT-2-104-580580

FT-2-105-580580

FT-2-127-580580

Other dimensions are available on request

Basic Filtration

Filter Papers

111

Wet-strengthened Filter Papers
For Qualitative Analyses

These qualitative filter papers are essentially used for analytical purposes and routine analyses, whenever no gravimetricanalyses are required. They are wet-strengthened and can be used for pressure and vacuum filtration. They are made ofrefined pulp and linters with an > 95 % α-cellulose content and are very pure with an ash content ≤ 0.1 %.

Typical Values
Grade

Weight(g/m2)

Thickness(mm)

Particle retention(μm)

Filtration(s)

Precipitates

Properties

1288

84

0.21

12 – 15

10

Coarse crystalline

Wide-pore, loose structure, fast filtering

1289

84

0.21

8 – 1220

Medium-fine crystalline

Medium- to wide-pore, medium fast filtering

1292

84

0.17

5–8

20

Fine crystalline

Medium dense, medium fast filtering

1290

84

0.15

3–5

100

Fine crystalline

Narrow-pore, dense, slow filtering

1291

84

0.15

2–3

180

Very fine crystalline

Fine-pore, dense, very slow filtering

293

80

0.15

1–2

300

Very fine crystalline

Very fine-pore, very dense, very slow filtering

Ordering Information			Filter Discs, 100 pieces∅ in mm

Grade 1288

Grade 1289

Grade 1290

Grade 1291

Grade 1292

Grade 293

55

FT-3-206-055

FT-3-207-055

FT-3-208-055

FT-3-209-055

FT-3-210-055

FT-3-211-055

70

FT-3-206-070

FT-3-207-070

FT-3-208-070

FT-3-209-070

FT-3-210-070

FT-3-211-070

90

FT-3-206-090

FT-3-207-090

FT-3-208-090

FT-3-209-090

FT-3-210-090

FT-3-211-090

110

FT-3-206-110

FT-3-207-110

FT-3-208-110

FT-3-209-110

FT-3-210-110

FT-3-211-110

125

FT-3-206-125

FT-3-207-125

FT-3-208-125

FT-3-209-125

FT-3-210-125

FT-3-211-125

150

FT-3-206-150

FT-3-207-150

FT-3-208-150

FT-3-209-150

FT-3-210-150

FT-3-211-150

185

FT-3-206-185

FT-3-207-185

FT-3-208-185

FT-3-209-185

FT-3-210-185

FT-3-211-185

240

FT-3-206-240

FT-3-207-240

FT-3-208-240

FT-3-209-240

FT-3-210-240			Folded Filters, 100 pieces∅ in mm

Grade 1288

Grade 1289

Grade 1290

Grade 1291

Grade 1292

110

FT-4-206-110

FT-4-207-110

FT-4-208-110

FT-4-209-110

FT-4-210-110

125

FT-4-206-125

FT-4-207-125

FT-4-208-125

FT-4-209-125

FT-4-210-125

FT-4-211-125

150

FT-4-206-150

FT-4-207-150

FT-4-208-150

FT-4-209-150

FT-4-210-150

FT-4-211-150

185

FT-4-206-185

FT-4-207-185

FT-4-208-185

FT-4-209-185

FT-4-210-185

FT-4-211-185

240

FT-4-206-240

FT-4-207-240

FT-4-208-240

FT-4-209-240

FT-4-210-240

FT-4-211-240			Grade 293

Sheets in 580 × 580 mm, 100 pieces

Grade 1288

Grade 1289

Grade 1290

Grade 1291

Grade 1292

Grade 293

FT-2-206-580580

FT-2-207-580580

FT-2-208-580580

FT-2-209-580580

FT-2-210-580580

FT-2-211-580580

Other dimensions are available on request

112

Basic Filtration

Filter Papers

High-Purity Filter Papers
For Qualitative Analyses

These paper grades are used for analytical purposes that require a low ash content. Grades 292 and 292a are especiallysuitable for soil analyses because they are low in nitrogen. For phosphate or sodium determination, we recommend
grades 131 and 132.

Typical Values
Grade

Weight(g/m2)

Thickness(mm)

Particle retention(μm)

Filtration(s)

Material

292

87

0.18

5–8

45

Cotton linters, low-nitrogen and nitrates, ash content ≤ 0.06 % according
to DIN 54370

292a

97

0.19

4–7

60

Cotton linters, low-nitrogen and nitrates, ash content ≤ 0.06 % according
to DIN 54370

132

80

0.17

5–7

55

Cotton linters and refined pulp, low-phosphate and low-potassium,
ash content < 0.02 % according to DIN 54370

131

80

0.16

3–5

100

Cotton linters and refined pulp, low-phosphate and low-potassium,
ash content < 0.02 % according to DIN 54370

Ordering Information			Filter Discs, 100 pieces∅ in mm

Grade 131

Grade 132

Grade 292

Grade 292a

55

FT-3-329-055

FT-3-205-055

FT-3-215-055

70

FT-3-329-070

FT-3-205-070

FT-3-215-070

90

FT-3-329-090

FT-3-205-090

FT-3-215-090

110

FT-3-329-110

FT-3-205-110

FT-3-215-110

FT-3-329-125

FT-3-205-125

FT-3-215-125

150

FT-3-329-150

FT-3-205-150

FT-3-215-150

185

FT-3-329-185

FT-3-205-185

FT-3-215-185

240

FT-3-329-240

FT-3-205-240

FT-3-215-240

125

			

FT-3-351-125

Folded Filters, 100 pieces∅ in mm

Grade 131

Grade 132

Grade 292

Grade 292a

110

FT-4-351-110

FT-4-329-110

FT-4-205-110

FT-4-215-110

125

FT-4-351-125

FT-4-329-125

FT-4-205-125

FT-4-215-125

150

FT-4-351-150

FT-4-329-150

FT-4-205-150

FT-4-215-150

185

FT-4-351-185

FT-4-329-185

FT-4-205-185

FT-4-215-185

FT-4-329-240

FT-4-205-240

FT-4-215-240

240

			

Sheets in 580 × 580 mm, 100 pieces

Grade 292

Grade 292a

FT-2-205-580580

FT-2-215-580580

Other dimensions are available on request

Basic Filtration

113

Filter Papers

Filter Papers

For Qualitative-Technical Analyses
These filter papers are used for routine analyses like clarification, determination of substances, but also as discs with acenter hole for technical applications. Grades with a wet burst resistance > 30 kPa are referred to as wet-strengthened andare ­therefore suitable for pressure or vacuum filtration. They are made of refined pulp and linters with an > 95 % α-cellulose­content, are very pure with an ash content between < 0.1 to 0.15 %. Below you will find an overview of the most commonlyused grades.

Typical Values
Grade

Surface

Weight(g/m²)

Thickness(mm)

Particle
Retention (µm)

Filtration(s)

Wet Burst
Resistance (kPa)

Properties

3 hw

Smooth65

0.14

8 – 1220

40

Medium fast filtering, filter paper for ­routine
work in the lab

4b

Smooth75

0.15

8 – 1222> 15

Medium fast filtering, filtration of coarse
precipitates, wick paper for seed testing

603/NCrêped

75

0.25

> 15

8

≥ 50

Fast filtering, filtration of sugar solutions

6

Smooth80

0.17

10 – 1315

30

Fast filtering, degassing beer before analysis,
clarification of spirits

100/N

Smooth

85

0.18

6–8

30

80

Medium fast filtering, ash content < 0.1 %,low potassium and sodium content,
determination of the sugar content

5 H/NCrêped

85

0.28

> 40

3

≥ 40

Very fast filtering, wide-pore, filtration of
essential oils

3 S/hSmooth

200

0.36

5–7

55

15

Medium fast to slow filtering, narrow-pore,re-wet test for diapers

114

Basic Filtration

Filter Papers

Ordering Information			Filter Discs∅ in mm

Grade 3 hw
(100 Pieces)

Grade 4 b(100 Pieces)55

FT-3-303-055

70

Grade 6(100 Pieces)

Grade 100/N(100 Pieces)

FT-3-309-055

FT-3-312-055

FT-3-328-055

FT-3-303-070

FT-3-309-070

FT-3-312-070

FT-3-328-070

90

FT-3-303-090

FT-3-309-090

FT-3-335-090

FT-3-312-090

FT-3-328-090

110

FT-3-303-110

FT-3-309-110

FT-3-335-110

FT-3-312-110

FT-3-328-110

125

FT-3-303-125

FT-3-309-125

FT-3-335-125

FT-3-312-125

FT-3-328-125

FT-3-423-125

FT-3-307-125

150

FT-3-303-150

FT-3-309-150

FT-3-335-150

FT-3-312-150

FT-3-328-150

FT-3-423-150

FT-3-307-150

185

FT-3-303-185

FT-3-309-185

FT-3-335-185

FT-3-312-185

FT-3-328-185

FT-3-423-185

FT-3-307-185

240

FT-3-303-240

FT-3-309-240

FT-3-335-240

FT-3-312-240

FT-3-328-240

FT-3-423-240

FT-3-307-240			Grade 603/N(100 Pieces)

Grade 5 H/N(100 Pieces)

Grade 3 S/h(50 Pieces)
FT-3-307-055

FT-3-423-090

FT-3-307-090
FT-3-307-110

Folded Filters, 100 pieces∅ in mm

Grade 3 hw

Grade 4 b

Grade 603/N

Grade 6125

FT-4-303-125

FT-4-309-125

FT-4-335-125

FT-4-312-125

150

FT-4-303-150

FT-4-309-150

FT-4-335-150

FT-4-312-150

185

FT-4-303-185

FT-4-309-185

FT-4-335-185

FT-4-312-185

240

FT-4-303-240

FT-4-309-240

FT-4-335-240

FT-4-312-240

FT-4-328-240

FT-4-423-240

270

FT-4-303-270

FT-4-309-270

FT-4-335-270

FT-4-312-270

FT-4-328-270

FT-4-423-270

320

FT-4-303-320

FT-4-309-320

FT-4-335-320

FT-4-312-320

FT-4-328-320

FT-4-423-320			Grade 100/N

Grade 5 H/N
FT-4-423-125

FT-4-328-150

FT-4-423-150
FT-4-423-185

Sheets in 580 × 580 mm, 100 pieces

Grade 3 hw

Grade 4 b

Grade 603/N

Grade 6

Grade 100/N

Grade 5 H/N

FT-2-303-580580

FT-2-309-580580

FT-2-335-580580

FT-2-312-580580

FT-2-328-580580

FT-2-423-580580

Other dimensions are available on request

Basic Filtration

Glass and Quartz Microfiber Filters

115

Glass Microfiber Filters
Without Binder

Binder-free glass microfiber filters are recommended for analytical and gravimetric analyses and also as prefilters. These filters combine fast flow rates withhigh load capacity and the retention of very fine particles; they are biologicallyinert, are resistant to most chemicals and withstand temperatures up to 500 °C(grade 550-HA up to 550 °C).

Typical Values
Grade

Weight(g/m2)

Thickness(mm)

Penetration
0.3 µm (%)*

Particle retention inliquids (µm)

Filtration speed(mL/min)Fulfills the requirements in
EN 872:2005 (weigh loss)

MGA

54

0.23

< 0.001

1.6

510

Yes

MGB

143

0.70

< 0.001

1.0

210

MGC

54

0.24

< 0.001

1.2

335

MGD

120

0.47

< 0.1

2.7

920

MGF

75

0.38

< 0.001

0.7

110

MGG

65

0.27

≤ 0.001

1.5

600

13440

88

0.44

0.7

120

MG 160

75

0.35

1.2

400

MG 550-HA

65

0.27

1.5

500

< 0.002

Yes

Yes* Measurement according to EN 143 (0.3 μm, 5.3 cm/s, paraffin oil)

Ordering Information
Filter Discs∅ in mm

MGA (100 pieces)

MG 160 (50 pieces)

21

MGB (50 pieces)

MGC (100 pieces)

MGD (50 pieces)

FT-3-1102-025

FT-3-1103-025

FT-3-1104-025

FT-3-1102-021

25

FT-3-1101-025

37

FT-3-1101-037

FT-3-01110-037

47

FT-3-1101-047

FT-3-01110-047

FT-3-1102-047

FT-3-1103-047

FT-3-1104-047

50

FT-3-1101-050

FT-3-01110-050

FT-3-1102-050

FT-3-1103-050

FT-3-1104-050

55

FT-3-1101-055

FT-3-1102-055

FT-3-1103-055

70

FT-3-1101-070

FT-3-01110-070

FT-3-1102-070

FT-3-1103-070

FT-3-1104-070

80

FT-3-1101-080

90

FT-3-1101-090

FT-3-01110-090

FT-3-1102-090

FT-3-1103-090

FT-3-1104-090

100

FT-3-1101-100

FT-3-01110-100

FT-3-1102-100

FT-3-1103-100

FT-3-1104-100

110

FT-3-1101-110

FT-3-01110-110

FT-3-1102-110

FT-3-1103-110

FT-3-1104-110

125

FT-3-1101-125

FT-3-1102-125

FT-3-1103-125

FT-3-1104-125

150

FT-3-1101-150

FT-3-1102-150

FT-3-1103-150

FT-3-1104-150

293

FT-3-1104-293

116∅ in mmBasic Filtration

MGF (100 pieces)

MGG (100 pieces)

24
25

Glass and Quartz Microfiber Filters

MG 550-HA (100 pieces)13440*

FT-3-01147-024
FT-3-1105-025

FT-3-1106-025

4213440--42------Q

44

13440--44------Q

47

FT-3-1105-047

FT-3-1106-047

FT-3-01147-047

13440--47------Q

50

FT-3-1105-050

FT-3-1106-050

FT-3-01147-050

13440--50------Q

55

FT-3-1105-055

FT-3-1106-055

FT-3-01147-055

70

FT-3-1105-070

FT-3-1106-070

FT-3-01147-070

90

FT-3-1105-090

FT-3-1106-090

FT-3-01147-090

100

13440-100------K

110

FT-3-1105-110

FT-3-1106-110

FT-3-01147-110

125

FT-3-1105-125

FT-3-1106-125

FT-3-01147-12513013440-130------K

150

FT-3-1105-150

293

FT-3-1105-293* Q = 500 pieces | K = 50 pieces
Other dimensions are available on request

FT-3-1106-150

13440-150------K
13440-293------K

Basic Filtration

Glass and Quartz Microfiber Filters

117

Glass Microfiber Filters
With Binder

These filters are mostly used either for monitoring air and gas or as a prefilter.
They are manufactured with synthetic binding agents to ensure that the filter has
a defined strength. They are mechanically and chemically stable, have a temperature resistance up to 180 °C and – depending on the binding agent used –are either hydrophobic or hydrophilic.

Typical Values
Grade

Weight(g/m2)

Thickness(mm)

Penetration
0.3 μm (%)*

Pressure drop
5.3 cm/s (Pa)Binding agent

MG 227/1/60

60

0.32

< 0.5

260

Hydrophobic

13430

220

1.25

0.02

360

Hydrophilic

13400

73

0.39

0.015

363

Hydrophilic

MG 400 XA

75

0.35

< 0.001

425

Hydrophobic

MG 1387/1

90

0.38

≤ 0.003400

Hydrophilic* Tested and classified according to the Standard EN 143

118

Basic Filtration

Glass and Quartz Microfiber Filters

Ordering Information
Filter Discs∅ in mm

MG 227/1/60(100 pieces)13430**

13400**MG 1387/1(50 pieces)

13

13400--13------S

20

13400--20------S

25

13400--25------Q

42

13400--42------Q

44

13400--44------Q

45

13400--45------Q

FT-3-01125-045

13400--47------Q

FT-3-01125-047

13400--50------Q

FT-3-01125-050

4713430--47------S

50
55

FT-3-01125-055

8013400--80------N

100

13430-100------K

13400-100------K

110

FT-3-01125-11012013400-120------K

124

13400-124------K

125

FT-3-01125-12512713430-127------K

13400-127------K

13013430-130------K

13400-130------K

142

13430-142------K

13400-142------K

150

FT-3-01124-150

293** K= 50 pieces, N= 100 pieces, Q = 500 pieces, S= 200 pieces
Other dimensions are available on request

13400-150------K
13430-293------K

13400-293------K

FT-3-01125-130

Basic Filtration

Glass and Quartz Microfiber Filters

119

Quartz Microfiber Filters
The quartz microfiber material of the Sartorius pre-heated filters, grade Q3400,
is made of high-purity quartz microfibers without any addition of glass microfibers or binding agents. In addition, the Q3400 filter grade is tempered toremove all chemically combined water and to give the filters excellent weightand dimensional stability. Sartorius filters are especially suitable for emissions
monitoring at temperatures of up to 900 °C and wherever filters of the highest
purity are needed.

Typical Values
Grade

Material

Weight(g/m²)

Thickness(mm)

Penetration,
0.3 μm 15 cm/s*

Temperature
Resistance

Q3400

100 % Quartz
microfiber silicium
dioxide (SiO2)

85

0.43

< 0.002

up to 900 °C* Tested and classified according to the Standard EN 143

Ordering Information			Filter Discs∅ in mm

Q3400

20Q3400--20------G

25

Q3400--25------G

30

Q3400--30------G

37

Q3400--37------G

45

Q3400--45------G

47

Q3400--47------G

50

Q3400--50------G

82

Q3400--82------N

90

Q3400--90------N

142

Q3400-142------K

150

Q3400-150------K* G = 25 pieces, K = 50 pieces, N = 100 pieces
Other dimensions as well as sheets are available on request

120

Basic Filtration

Membrane Filters

Membrane Filtration – Quick Selection Guide

SampleHighly Particleladen Solutions

Prefiltration
Depth filters (glass microfiber filters | filter papers)
Membranes, pore-sizes 8 – 0.8 μm
(→ CN, CA)Solvents

Aqueous

Air		 RC
Regenerated Cellulose		Particle Removal

		CN
		 Cellulose Nitrate		Particle Retention and Cell Capture		PTFE		Polytetra-fluoroethylene
		Sterile Venting

		PA		 Polyamide | Nylon
		Particle Removal

		CA
		 Cellulose Acetate		Particle Retention and -Removal

		CN
		 Cellulose Nitrate		Particle- and Cell Retention

		PTFE		Polytetra-fluoroethylene
		Particle Removal

		RC
		 Regenerated Cellulose		
General Purpose Filtration

		CA		 Cellulose Acetate		Particle Retention

		PES
		Polyethersulfone
		Cell Culture Media, Buffers

		PC-TEM
		 Polycarbonate Track-etched
Membrane		
Air Monitoring

Solvent-Mixtures
		PES
		Polythersulfone
		
Pharmaceutical Solutions
		RC
		 Regenerated Cellulose		Particle Removal, De-gassing		PC-TEM
		 Polycarbonate Track-etched
Membrane		Particulate- and Cell Analysis

		PA
		Polyamide|Nylon
		Particle Removal, Alkaline Solutions
		PC-TEM
		 Polycarbonate Track-etched Membrane		Particulate Analysis
		PTFE		Polytetra-fluoroethylene
		Particle Removal

		 polar = hydrophilic		non-polar = hydrophobichydrophobic membranes need to be
pre-wetted with ethanol or methanol
before the filtration of aqueous solutions

Basic Filtration

Membrane Filters

121

Cellulose Nitrate (Mixed Cellulose Ester)
Cellulose nitrate membrane filters are indicated for many general laboratory
applications where a membrane with a high non-specific adsorption is suitable.
They are hydrophilic, have high flow rates thanks to their symmetric structure
and are ­compatible with aqueous solutions (pH 4 to 8), hydrocarbons andseveral other organic solvents. The cellulose nitrate ­membranes are available indifferent pore sizes from 0.2 µm to 8 µm.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Water Flow Rate(mL/min/cm2/bar)

Burst Pressure
(bar)

11327

0.2

130

4.2

25

≥ 0.35

11306

0.45

130

2.4

70

≥ 0.3

11305

0.65

130

2

130

≥ 0.25

11304

0.8

130

1.4

200

≥ 0.2

11303

1.2

130

1

200

≥ 0.2

11302

3

130

0.5

430

≥ 0.2

11342

5

130

0.5

570

≥ 0.15

11301

8

130

0.3

750

≥ 0.1

** Measurement according to EN 143 (0.3 μm, 5.3 cm/s, paraffin oil)

Ordering Information
Filter Discs∅ in mm11301 (8 µm)*

11302 (3 µm)*

11303 (1.2 µm)*

11304 (0.8 µm)*

1311301--13------N

11302--13------N

11303--13------N

11304--13------N

20

11304--20------N

25

11301--25------N

37

11301--37------N

4711301--47------N

11302--47------N

11303--47------N

11304--47------N

50

11301--50------N

11302--50------N

11303--50------N

11304--50------N

7011301--70------G
11302--90------G

11303--90------G

11304--90------G

11302-100------G

11303-100------G

11304-100------G

90
100

11301-100------N

11302--25------N

11303--25------N

11304--25------N
11304--37------N

122

Basic Filtration

Membrane Filters∅ in mm

11305 (0.65 µm)*

11306 (0.45 µm)*

11327 (0.2 µm)*

11342 (5 µm)*

1311305--13------N

11306--13------N

11327--13------N

11342--13------N

11327--25------N

11342--25------N

11327--47------N

11342--47------N

20
25

11306--20------N
11305--25------N

37

11306--25------N
11306--37------N

4711305--47------N

11306--47------N

50

11305--50------N

11306--50------N

11342--50------N

8511306--85------N

9011306--90------N

11342--90------G

11306-100------N

11342-100------G

100

11305-100------N

110* G = 25 pieces, N = 100 pieces
Other dimensions and packaging units are available on request

11306-110------N

Basic Filtration

Membrane Filters

123

Cellulose Acetate
Cellulose acetate membranes combine high flow rates and thermal stability withvery low adsorption characteristics, and are therefore excellently suited for use
in pressure filtration devices. They are hydrophilic, have high flow rates thanks totheir symmetric structure and are compatible with aqueous solutions (pH 4 – 8),
oils, alcohols and other organic solvents. The 0.2 µm membrane is the filter ofchoice for sterile filtration of aqueous solutions, such as nutrient media, buffers
and sera. The cellulose acetate membranes are available in different pore sizes
from 0.2 to 5 µm.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Water Flow Rate(mL/min/cm2/bar)

Burst Pressure
(bar)

11107

0.2

120

2.9

24

0.8

11106

0.45

120

1.9

69

0.7

11105

0.65

120

1.5

115

0.7

11104

0.8

120

1

200

0.5

12303

1.2

140

0.8

320

0.4

12342

5

140

0.4

570

0.25

Ordering Information
Filter Discs∅ in mm11104 (0.8 µm)*

1311104--13------N

25

11104--25------N

11105 (0.65 µm)*

11105--25------N

30
3711104--37------N

11106 (0.45 µm)*

11107 (0.2 µm)*

11106--13------N

11107--13------N

11106--25------N

11107--25------N

11106--30------N

11107--30------N

12303 (1.2 µm)*

12342 (5 µm)*

12303--25------N

12342--25------N

12342--47------N

11106--37------N

45
47

11104--47------N

11105--47------N

11106--47------N

11107--47------N

12303--47------N

50

11104--50------N

11105--50------N

11106--50------N

11107--50------N

12303--50------N

7011106--85------N

85
9011104--90------N

11105--90------G

11106--90------G

11107--90------G

100

11106-100------N

11107-100------N

11011106-110------N* G = 25 pieces, N = 100 pieces
Other dimensions and packaging units are available on request

12303-100------G

124

Basic Filtration

Membrane Filters

Regenerated Cellulose
The very low adsorption membranes are hydrophilic, solvent-resistant (pH 3 – 12)
and therefore suited for the particle removal from solvents. The membrane isreinforced with nonwoven cellulose. They are available in two pore sizes: 0.45 µm
and 0.2 µm.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Water Flow Rate(mL/min/cm2/bar)

18407

0.2

170

4.4

15

18406

0.45

170

2.9

30

Ordering Information
Filter Discs∅ in mm

18406 (0.45 µm)*

18407 (0.2 µm)*

1318406--13------N

18407--13------N

25

18406--25------N

18407--25------N

47

18406--47------N

18407--47------N

50

18406--50------N

18407--50------N

90

18406--90------G

100

18406-100------G

18407-100------G

142

18406-142------G

18407-142------G

293

18406-293------G

18407-293------G* G = 25 pieces, N = 100 pieces
Other dimensions and packaging units are available on request

Basic Filtration

Membrane Filters

125

Polyethersulfone
Polyethersulfone (PES) membrane filters are hydrophilic, have high flow rates, alow non-specific protein adsorption and are chemically resistant over a pH rangeof 1 – 14. They are therefore recommended for the filtration of aqueous solutions
as well for protein filtration. Furthermore, the low level of extractables makes
them suitable for environmental analysis.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Water Flow Rate(mL/min/cm2/bar)

Burst Pressure
(bar)

15458

0.1

150

3.8

10

≥ 0.6

15407MI

0.2

150

3.5

25

≥ 0.5

15406

0.45

150

2.6

46

≥ 0.5

Ordering Information
Filter Discs∅ in mm

15406 (0.45 µm)*

15407MI (0.2 µm)*

15458 (0.1 µm)*

2515406--25------N

15407--25----MIN

15458--25------N

47

15406--47------N

15407--47----MIN

15458--47------N

50

15406--50------N

15407--50----MIN

15458--50------N

90
142
293

15407--90----MIK
15406-142------G

15407-142----MIG

15458-142------G

15407-293----MIG

15458-293------G* G = 25 pieces, K = 50 pieces, N = 100 pieces
Other dimensions and packaging units are available on request

126

Basic Filtration

Membrane Filters

Polyamide
Polyamide membrane filters are hydrophilic and chemically resistant to alkaline
solutions and organic solvents. They are therefore recommended for the particle
removal from aqueous solutions and solvents for analytical determination suchas HPLC, for the sterile filtration of these liquids as well as for applications wherea membrane with a relatively high non-specific adsorption is suitable.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Water Flow Rate(mL/min/cm2/bar)

Burst Pressure
(bar)

25007

0.2

115

3.2

15

≥ 0.25

25006

0.45

115

2.3

35

≥ 0.23

Ordering Information
Filter Discs∅ in mm

25006 (0.45 µm)*

25007 (0.2 µm)*

1325006--13------N

25007--13------N

25

25006--25------N

25007--25------N

47

25006--47------N

25007--47------N

50

25006--50------N

25007--50------N

90

25006--90------G

25007--90------G

142

25006-142------N

25007-142------N

293

25006-293------N

25007-293------N* G = 25 pieces, N = 100 pieces
Other dimensions and packaging units are available on request

Basic Filtration

Membrane Filters

127

Hydrophobic PTFE
The main application of these membrane filters is the filtration of air, gases or
chemicals. They are made of PTFE (polytetra-fluorethylene) only and are therefore permanently hydrophobic. Unlike other (hydrophilic) filter types, they are
not wetted by air humidity, allowing ­unhindered passage of air at low ­differential
pressures as well. PTFE ­membrane filters have an excellent ­chemical compatibility (pH 1 to 14), so that they are also used for the filtration of solvents and acids,
to which other filter types are not resistant. Due to their hydrophobic characteristics, they must be pre-wetted with ethanol or methanol before the filtration of
aqueous media.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Isopropanol Flow Rate(mL/min/cm2/bar)

11807

0.2

65

1.4

11

11806

0.45

80

0.9

20

11803

1.2

100

0.45

80

11842

5

100

0.10

250

11842 (5 µm)*

Ordering Information
Filter Discs∅ in mm

11803 (1.2 µm)*

11806 (0.45 µm)*

11807 (0.2 µm)*

1311803--13------N

11806--13------N

11807--13------N

25

11803--25------N

11806--25------N

11807--25------N

37

11803--37------N

11806--37------N

4211842--25------N

11842--42------N

47

11803--47------N

11806--47------N

11807--47------N

11842--47------N

50

11803--50------N

11806--50------N

11807--50------N

11842--50------N

90

11803--90------G

11806--90------G

11807--90------G

100

11803-100------G

11806-100------G

11807-100------G

11842-100------G

142

11803-142------G

11806-142------G

11807-142------G

11842-142------G

293

11803-293------G

11806-293------G

11807-293------G

11842-293------G* G = 25 pieces, K = 50 pieces, N = 100 pieces
Other dimensions and packaging units are available on request

128

Basic Filtration

Membrane Filters

Polycarbonate Track-Etched
Those white and hydrophilic polycarbonate track-etched filters are manufactured from high grade polycarbonate film using track-etch technology. Their
capillary pore structure is uniform and precise, with a narrow pore size distribu­
tion to retain particles on their surface. Track-etched filters are an excellent
choice for accurate fractionation of particulates because of their precise poresize. Track-etch technology offers the user distinct performance advantages when
excellent surface capture and high sample visibility are required. Their main applications are particulate analysis, epifluorescence microscopy, fluid clarification,
cytology, cell biology, bioassays, water microbiology and environmental analysis.

Typical Values
Type

Pore Size(µm)

Thickness(µm)

Bubble Point(bar)

Water Flow Rate(mL/min/cm2/0.7 bar)

Burst Pressure
(bar)

23058

0.1

25

7.0

≥ 0.5

≥ 0.7

23007

0.2

25

3.5

≥ 10

≥ 0.7

23006

0.4

25

2.0

≥ 30

≥ 0.7

23004

0.8

25

0.6

≥ 40

≥ 0.7

Ordering Information
Filter Discs, 100 Pieces∅ in mm23004 (0.8 µm)

23006 (0.4 µm)

2523004--25------N 23006--25------N 23007--25------N

23058--25------N

47

23006--47------N 23007--47------N

23058--47------N

50

23007--50------N

Other dimensions and packaging units are available on request

23007 (0.2 µm)

23058 (0.1 µm)

Basic Filtration

Blotting | Chromatography Papers

129

Blotting | Chromatography Papers
These papers are made of cotton linters only with α-cellulose content of > 98 %.
These highly pure papers are not only ideal for blotting and chromatography,
but also for a wide range of absorption applications like those common in the life
sciences and ­diagnostics. Below you will find an overview of the most commonlyused grades.

Typical Values
Grade

Weight(g/m²)

Thickness(mm)

Capillary Rise
(mm/30 min)Capillary Rise
(mm/10 min)

Properties

FN 4

125

0.24

95

Chromatography paper, ash content < 0.04 %

FN 7

150

0.32

145

Chromatography paper, ash content < 0.04 %

FN 30

320

0.90

240

Chromatography paper, ash content < 0.04 %,paper for antibiotic test strips

FN 100

195

0.35

115

70

The most commonly used chromatography andblotting paper

BF 3

330

0.76

30

130

Blotting paper to increase and maintain thetransport of liquids

Ordering Information
Sheets in 580 × 600 mm
Grade FN 4(100 Sheets)

Grade FN 7
(50 Sheets)

Grade FN 30(25 Sheets)

Grade FN 100(50 Sheets)

Grade BF 3
(50 Sheets)

FT-2-504-580600N

FT-2-507-580600K

FT-2-526-580600G

FT-2-527-580600K

FT-2-520-580600K

Other dimensions and packaging units are available on request

130

Basic Filtration

Blotting | Chromatography Papers

Nitrocellulose Membrane for Blotting
Sartorius nitrocellulose membranes are available in two pore sizes, 0.22 μm and0.45 μm. Both versions combine the advantages of high protein binding capacitywith low background and high membrane stability, which ensures easy handling.
Due to its large surface area, the 0.22 μm membrane version is recommended
for small proteins. Sartorius blotting membranes are ideal for western blotting,
DNA blotting as well as dot or slot blots. They have been optimized for all protein
blotting systems, such as electrotransfer, semi-dry or simple capillary blotting.

Typical Values
0.22 µm0.45 µm

Material

Cellulose nitrate

Cellulose nitrate

Thickness

130 μm130 μmWater flow rate

27 mL/(min. cm2 bar)

70 mL/(min. cm2 bar)

Bubble point

4.4 bar

2.4 barWettability in water

≤1s

≤1s

Extractable content in water

≤1%

≤1%

Burst pressure

0.8 bar

0.2 barBinding capacity for IgG

200 µg/cm2

200 µg/cm2

Ordering Information
Roll Size

Order No.

NC 0.22 µm

30 cm × 3 m

11327------41BL

NC 0.45 µm

30 cm × 3 m

11306------41BL

All indicated data to be understood as typical average values

Basic Filtration

Filtration Equipment

131

Re-usable 13 mm Syringe Filter Holders
For the Ultracleaning of Small Volumes Up to About 10 mL

PTFE Holder for Solvents and Chemicals
Made completely of PTFE, this holder is unaffected by chemicals and contains
no trace elements which could be released into the liquid being filtered. It istherefore extremely well suited for particle removal from samples and reagentsfor analytical methods, such as NMR samples. Other benefits of this application
are the low hold-up volume, the easy cleaning and the drying at a temperatureof 180 °C. The construction of the holder ensures leak proof sealing without asealing ring, and avoids twisting of the membrane filter when the top is tightened
onto the base.

Specifications
Connectors

Female Luer Lock inlet, luer slip outlet

Chemical compatibility

As for PTFE

Filtration area

0.5 cm2

Materials

PTFE top and bottom parts

Max. operating pressure

5 bar | 500 kPa | 72.5 psi

Membrane filter ∅13 mmSterilization

By autoclaving (max. 134 °C) or by dry heat (max. 180 °C)

Hold-up volume

Less than 0.03 mL after overcoming the bubble point (0.3 mL before)

Ordering Information
Description

Order No.

13 mm PTFE Syringe Filter Holder

16574

Polycarbonate Holder for Aqueous Solutions
This inexpensive filter holder is made of clear, autoclavable polycarbonate.
The silicone gasket enables a leak-free ­filtration at pressures of up to 7 bar by­simply screwing it together manually. Filter supports in the top and bottom parts
allow filtration in either direction.

Specifications
Connectors

Female Luer Lock inlet, luer slip outlet

Chemical compatibility

As for polycarbonate and silicone

Filtration area

0.5 cm2

Materials

Polycarbonate top and bottom part,silicone gasket

Max. operating pressure

7 bar | 700 kPa | 101.5 psi

Membrane filter ∅13 mmSterilization

By autoclaving at 121 °C

Hold-up volume

Less than 0.2 mL after overcoming the bubble point (0.3 mL before)

Ordering Information
Description

Order No.

13 mm PTFE Syringe Filter Holder

16574

132

Basic Filtration

Filtration Equipment

Re-usable 25 mm Syringe Filter Holders

For the Ultracleaning and Sterilizing Filtration of Volumes of Up to About 100 mL
Stainless Steel Holder for Solvents and Chemicals
Made of stainless steel, this holder is heat-resistant, and the chemical compatibility depends only on the inserted filter type. The top part can easily be mounted
on the bottom part using the enclosed tightening tool. Filter supports in the top
and bottom parts allow filtration in either direction.

Specifications
Connectors

Female Luer Lock inlet, luer slip outlet

Chemical compatibility

As for stainless steel

Filtration area

3 cm2

Materials

Stainless steel (1.4305) top and bottom parts

Max. operating pressure

7 bar | 700 kPa | 101.5 psi

Membrane filter ∅25 mmSterilization

By autoclaving (max. 134 °C) or by dry heat (max. 180 °C)

Hold-up volume

Less than 0.1 mL after overcoming the bubble point (0.3 mL before)

Ordering Information
Description

Order No.

25 mm Stainless Steel Holder

16214

Tightening tool, Polyman 24/5

6980595

Polycarbonate Holder for Aqueous Solutions
This inexpensive filter holder is made of clear, autoclavable polycarbonate. The
silicone gasket enables a leak-free filtration at pressures of up to 7 bar by simply­screwing it together manually. Filter supports in the top and bottom parts allow
filtration in either direction.

Specifications
Connectors

Female Luer Lock inlet, luer slip outlet

Chemical compatibility

As for polycarbonate and silicone

Filtration area

3 cm2

Materials

Polycarbonate top and bottom parts, silicone gasket

Max. operating pressure

7 bar | 700 kPa | 101.5 psi

Membrane filter ∅25 mmSterilization

By autoclaving at 121 °C

Hold-up volume

Less than 0.3 mL after overcoming the bubble point (0.6 mL before)

Ordering Information
Description

Order No.

25 mm Polycarbonate Syringe Filter Holder, pack of 1216517----------E

Silicone gasket, 20.5 × 26.5 × 0.5 mm, pack of 106980570

Basic Filtration

Filtration Equipment

133

25 mm Glass Vacuum Filter Holder

For Hybridization Tests, Particle Testing and Clarification
This filter holder is available in two versions differing from each other only in thetype of the filter support. The filter with glass frit ensures uniform distribution ofretained particles and is therefore recommended when the residue on the filter­surface is of interest. Because it is easy to clean, the device with the PTFE-coated screen support is preferable when the filtrate is required, or when liquids
difficult to remove from glass frits must be examined.
The PTFE ring, which holds the glass frit and the screen support, allows for the
autoclaving of the devices with a filter in position and protects the edge of theglass frit from breakage and potential leakage. It has a rim around the upper edge
to simplify the positioning of the membrane filter when inserted and a silicone
O-ring in the underside for a leak-proof seal on the filtrate side. The funnel-shaped
top part ­simplifies filling in the sample.

Specifications
Outlet spout

12 mm ∅

Parts and materials

Borosilicate glass funnel and base
PTFE | glass filter support (type 16306) or
PTFE | stainless steel filter support, coated withPTFE (type 16315)
Silicone O-ring 25 × 3 mm
Anodized Aluminium clamp

Chemical compatibility

As for glass, PTFE and silicone. The silicone O-ring can be
replaced by a fluoroelastomer O-ring (order no. 00118)

Funnel capacity

30 mL

Filtration area

3 cm2

Max. operating pressure

Only for vacuum

Suitable membrane filter ∅25 mm (or 24 mm)

Sterilization

By autoclaving (max. 134 °C) or by dry heat (max. 180 °C)

Ordering Information
6980115
Glass funnel
30 mL

6983008
Small clamp

17148
PTFE ring withglass frit and asilicone O-ring

17149
PTFE ring with
PTFE-coatedunderdrain
screen and support
screen including asilicone O-ring

6980116
Glass base6980117
Silicone O-Ring

Note: PTFE rings in sets 17148 and 17149 have different dimensions and are not interchangeable.

Description

Order No.

Glass vacuum filtration holder for 25 mm(or 24 mm) ­membrane filter, with glass frit­filter support

16306

Glass vacuum filtration holder for 25 mm
16315(or 24 mm) m­ embrane filter, with PTFE-coatedscreen filter support
Replacement parts are shown in the diagram.

134

Basic Filtration

Filtration Equipment

50 mm Glass Vacuum Filter Holder

For Particle Testing or Clarification and Sterile Filtration
This filter holder is available in two versions differing from each other only in thetype of the filter support. The device with glass frit ensures uniform distributionof retained particles and is therefore recommended, when the residue on thefilter surface is of interest. Because it is easy to clean, the device with the PTFE-­
coated screen support is preferable when the filtrate is required, or when liquids
difficult to remove from glass frits must be examined.
The PTFE ring, which holds the glass frit and the screen support, allows the autoclaving of the devices with a filter in position and protects the edge of the glass
frit from breakage and potential leakage. It has a rim around the upper edge
to simplify the positioning of the membrane filter when inserted and a silicone
O-ring in the underside for a leak-proof seal on the filtrate side.

Specifications
Outlet spout

15 mm ∅

Parts and materials

Borosilicate glass funnel and base
Silicone caoutchouc lid
PTFE | glass filter support (type 16307) or PTFE | stainlesssteel filter support, coated with PTFE (type 16316)
Silicone O-ring 45 × 3 mm
Anodized Aluminium clamp

Chemical compatibility

As for glass, PTFE and silicone. The silicone O-ring can be
replaced by a fluoroelastomer O-ring (order no. 00124).

Funnel capacity

250 mL

Filtration area

12.5 cm2

Max. operating pressure

Only for vacuum

Suitable membrane filter ∅50 mm (or 47 mm)

Sterilization

By autoclaving (max. 134 °C) or by dry heat (max. 180 °C)

Ordering Information
6980121
Lid6980119
Glass funnel,
250 mL6983006
Glass frit

17146
PTFE ring withglass frit and asilicone O-ring

6980120
Glass base6983009
Large clamp

6983007
PTFE-coatedsupport screen
6983010
Drain screen
6980117
Silicone O-Ring
17147
PTFE ring with
PTFE-coatedunderdrain
screen and support
screen including
a silicone O-ring

Note: PTFE rings in sets 17146 and 17147 have different dimensions and are not interchangeable.

Description

Order No.

Glass vacuum filtration holder
for 50 mm (or 47 mm) membrane filter,with glass frit filter support

16307

Glass vacuum filtration holder
for 50 mm (or 47 mm) membrane filter,with PTFE-coated screen filter support

16316

Replacement parts are shown in the diagram.

Basic Filtration

Filtration Equipment

135

All-Glass Vacuum Filter Holder

For Analytical Determinations, Particle Removal from Solvents
All areas, where liquid and device can come into direct contact, are made ofglass or PTFE. The device, in combination with ­solvent-resistant, hydrophilic
RC-membranes, is therefore ideal for ultracleaning and degassing solvents andsolvent mixtures for HPLC, GC and AA.
Convenience of handling is ensured by several beneficial features. A 6 mm wide
non-ground rim above the ground glass neck of the suction flask prevents thefiltrate from contacting grease on the ground glass surface and so avoids its
contamination while being poured out of the flask. The hose nipple connector
is made of polypropylene for safe connection of the vacuum hose. The filtrateoutlet spout ends well below the entrance to this hose nipple.

Specifications
Outlet spout

Borosilicate glass funnel, base and flask, ­sintered glass frit ina PTFE ring and fluoroelastomer O-ring (45 × 3 mm)
underneath, anodized ­aluminium clamp

Parts and materials

As for glass and PTFE

Chemical compatibility

250 mL

Funnel capacity

1 liter

Filtration area

12.5 cm2

Max. operating pressure

Only for vacuum

Suitable membrane filter ∅50 mm (or 47 mm), 40 or 42 mm prefilter

Sterilization

By autoclaving (max. 134 °C) or by dry heat (max. 180 °C)

Ordering Information
6980119
Glass funnel
6983006
Glass frit00124
Fluorelastomer
gasket6983009
Large clamp

6983004
Completefilter support
6983003
Polypropylene
connector
incl. gasket
69830056983002
Glass baseDescription

Order No.

All-glass vacuum filter holder
for 50 mm (or 47 mm) membrane filter,with vacuum-resistant flask, capacity 1 liter

16309

Replacement parts are shown in the diagram.

136

Basic Filtration

Filtration Equipment

Polycarbonate In-Line Filter Holder

For the Filtration of Liter Volumes of Aqueous Solutions
This holder is made of stable, autoclavable polycarbonate. This practical holder issuitable for many simple laboratory filtrations. It can be connected to a peristaltic
pump or a pressure container. The bell-shaped base protects the filtrate fromrepeated contamination while flowing in a receiver.
The holder is characterized by an excellent resistance to pressure and density
setting by simple hand-tightening. The transparent top part allows the visual
control of the correct fit of the O-ring. The hose nipples can be replaced by luer
connectors to use it as a large area syringe filter holder.

Specifications

6985004
connector

Chemical compatibility

As for polycarbonate, polypropylene andsilicone

Filtration area

12.5 cm2

Weight83 g

Threads for connectors

M 12 × 1 female thread

Materials

Polycarbonate top part, base part and hosenipple, polypropylene filter support, silicone
O-ring (40 × 5 mm)

Max. operating pressure

7 bar | 700 kPa | 101.5 psi

Suitable membrane filter ∅50 mm (40 or 42 mm prefilter)

Sterilization

By autoclaving at 121 °C
The material withstands repeated cycles,
provided aggressive cleaning agents are­completely washed off and that the boiler water
does not contain anti-corrosive or anti-scaling
additives.

00423
upper part

6980232
filter support
6980110
silicone O-ring6980232
filter support

6980383base part

6985004
connector

Ordering Information
Description

Order No.

Polycarbonate in-line filter holder for 50 mm
membrane filter, pack of 5.

16508----------B

Replacement parts are shown in the diagram.

Basic Filtration

Filtration Equipment

137

25 mm Stainless Steel Filter Holder
For In-Line Filtration

The G¼ connection threads with density barrel, guarantee leak-proof sealing of
the hose nipple and the holder without sealing rings. Other connectors, available
as accessories, fit the holder onto reducing valves or pumps with G¼ female
thread (order no. 01030) or G⅜ female thread order no. 01029) or onto pressuretanks with G⅜ male thread (order no. 00177).

Specifications

6980176
Connector

6981031
Top part
6981033
SiliconeO-ring6981032
Base part

6981034
Closing ring6980176
Connector

00177
G3/8 malethread01030
G1/4 female
thread01029
G3/8 female
threadConnectors

Hose nipples DN10

Filtration area

3 cm2

Weightca. 170 g

Materials

Stainless steel, except silicone
O-ring (21 × 2 mm) and aluminium closing ring

Max. operating pressure

5 bar | 500 kPa | 72.5 psi

Suitable membrane filter

25 mm (20 mm prefilter for the filtrationof liquids only)

Sterilization

By autoclaving (max. 134 °C) or
by dry heat (max. 180 °C)

Ordering Information
Description

Order No.

Stainless steel pressure filter holder for
25 mm ∅ membrane filter.

16251

Replacement parts are shown in the diagram.

138

Basic Filtration

Filtration Equipment

47 mm Stainless Steel Filter Holder
For In-Line Filtration

The filter holder is suitable for a pressure of up to 20 bar. The inlet side valve isconvenient for the intermittent run-off of waste water. Other connectors, available as accessories, fit the holder onto reducing valves or pumps with G⅜ female
thread (order no. 17089) or onto pressure tanks with G⅜ male thread (order no.
17069) or on taps with G¾ male thread (order no. 17068).

Specifications
Connectors

Hose nipples DN10

Connection thread

M12 × 1

Filtration area

13 cm2

Weightca. 490 g

Materials

Stainless steel, except silicone O-ring(42 × 3 mm), PTFE and fluoroelastomer
valve seals

6980717
fluoroelastomer
O-ring, 3 × 1.5 mm

Max. operating pressure

20 bar | 2,000 kPa | 290 psi

Suitable membrane filter

47 mm (40 or 42 mm prefilter)

6982005
closing ring

Sterilization

By autoclaving (max. 134 °C) or
by dry heat (max. 180 °C)

6980801
Stainless Steel nipple for 10 mm hose
6980722
Vent valve
6980656
PTFE flat gasket

6982003
top part
6980721
PTFE-coated backpressure screen

Ordering Information

6980178
silicone O-ring,
42 × 3 mm

Description

Order No.

Stainless steel filter holder for 47 mm membranefilter (with adapter M12 × 1 male thread to hosebarb DN10, Mat. 316, ref. 6980801)– Replacement parts are shown in the diagram

16254

Stainless steel filter holder for 47 mm membranefilter (with adapter M12 × 1 male thread to hosebarb DN 4 to 5, Mat. 316, ref. 6981132)

16278

Stainless steel back pressure screen

6980721-----1

Stainless steel filter support screen

6980180-----1

Stainless steel underdrain screen

00181

Stainless steel connector M12 × 1 male thread
to hose barb DN 4 – 56981132

Adapter Quick connect nipple length 60 mm
male part to male thread M12 × 1, Mat 316

17090-----1

6980180
PTFE-coated filter
support screen
6980737
PTFE-coatedunderdrain screen
6982006base plate6980801
Stainless steel nipplefor 10 mm hose

Diagram for 16254

Basic Filtration

Filtration Equipment

139

Stainless Steel Pressure Filter Holder
For the Filtration of Up to 5 L Volumes

A practical filter holder for many laboratory filtrations. It can be attached to atripod with the help of a steel rod which can be screwed in. The hose nipple isscrewed into the side of the top part, leaving room for a large filling opening.
This makes pouring in the sample easier, and the sample can be refilled without
removing the tube connection to the pressure source.
Leak-proof sealing is achieved by hand-tightening the closing ring. For thefiltration of small volumes (up to about 200 mL of soil samples or viscous liquids,
such as oils), the holder is connected directly to a pressure source. For the filtration of up to 5 L volumes of relatively easily filterable liquids (e.g. buffer solutions,solutions for cell counters and tissue culture solutions), it is used in combination
with a pressure tank.

Specifications

6986055PTFE stopperfor 6980151
6986084
top part
6982020
siliconeO-ring,41 × 2 mm
6982005
closing ring6986083
barrel(200 mL)

6980737
PTFE-coatedunderdrain
screen6980151
Filling port
closure, complete withPTFE stopper

6981288
Steel rod

Chemical compatibility

As for stainless steel, PTFE and silicone.
If required, the silicone O-ring in the filter
support can be replaced by a fluoroelastomer
O-ring 00179 or a PTFE O-ring 17038(by reducing the max. operating pressure
to 4 bar | 58 psi); the silicone O-ring in the top
part can be replaced by a fluoroelastomer
O-ring 17145.

Filtration area

13 cm2

Weight960 g

Threads for connectors

M 12 × 1 female thread

Materials

Top part, barrel, base part, corrugated iron,closing ring, closure cap, back pressure screen
and stainless steel hose nipples 1.4401 (AISI
316), PTFE-coated stainless steel filter support,
silicone O-rings, 41 × 2 mm (top part) and42 × 3 mm (filter support), PTFE-sealing (cap).

Max. operating pressure

10 bar | 1,000 kPa | 145 psi

Suitable membrane filter ∅47 mm (40 or 42 mm prefilter)

Sterilization

By autoclaving (max 134 °C) or
by dry heat (180 °C)

6980801
Hose nipple

6980178
siliconeO-ring,42 × 3 mm
6980180
PTFE coatedfilter support
screen6982006base part6980801
Hose nipple

Ordering Information
Description

Order No.

Stainless steel pressure filter holder

16249

Stainless steel pressure filter holder
with double jacket

16249-----3

Replacement Parts
Description

Order No.

Fluoroelastomer O-ring, 42 × 3 mm

00179

PTFE O-ring, 42 × 3 mm

17038

Fluoroelastomer O-ring for upper part, 41 × 2 mm

17145

Other replacement parts are shown in the diagram or on page 138.

140

Basic Filtration

Filtration Equipment

Chemical-resistant PTFE Filter Holder
For the Filtration of Aggressive Liquids

The holder hinders the release of trace elements into the filtrate and is resistantto almost all chemicals. The fluoroelastomer O-ring in the top part allows easy
hand tightening, and can be replaced by a PTFE O-ring, order no. 17039. The
6 mm outlet nipple is an integral part of the base, the 10 mm inlet hose nipple canbe replaced by a G⅜ connector, order no. 17051.

Specifications

6985002PTFE
connector

6985011fluoroelastomer
O-ring,39 + 3.5 mm

17051

Chemical compatibility

As for PTFE and fluoroelastomer

Filtration area

12.5 cm2

Thread for inlet connector

M 14 × 1.5 male thread

Materials

Top part, barrel, base part: corrugated iron,hose nipples and filter support with40 × 3.5 mm O-ring: PTFE,
locking rings: aluminium
39 × 3.5 mm fluoroelastomer O-ring (top part)Max. operating pressure

5 bar | 500 kPa | 72.5 psi

Suitable membrane filter ∅47 mm

Sterilization

By autoclaving (max 134 °C) or
by dry heat (180 °C)

Ordering Information

6985000
PTFE O-ring,
40 + 3.5 mm
6985001
PTFE filter
supportscreen,47 mm

Description

Order No.

PTFE pressure filter holder, 47 mm,with 200 mL capacity.

16579

Replacement Parts
Description

Order No.

PTFE O-ring, 39 × 3.5 mm

17039

Other replacement parts are shown in the diagram.

Basic Filtration

Combisart® Manifolds

141

Combisart® Manifolds
1-, 3- and 6-Branch

Made of high-grade stainless steel (B.S. 304S3 | AISI 304); accommodates any
type of vacuum funnel. Stainless steel three-way valves (taps) allow the vacuum
for each filter holder to be individually controlled and each holder to be sterilely
vented. The low height of the manifold ports is particularly advantageous forworking on a clean bench.

Ordering Information
Combisart® Manifolds, without Base Support and Frit

Order No.

Combisart 1-branch manifold

16844

Combisart 3-branch manifold

16842

Combisart 6-branch manifold

16843

Combisart® Sets, Stainless Steel Capacity

Order No.

1-branch 1 × 100 mL

16844-CS

1-branch 1 × 500 mL

16845-CS

3-branch 3 × 100 mL

16824-CS

3-branch 3 × 500 mL

16828-CS

6-branch 6 × 100 mL

16832-CS

6-branch 6 × 500 mL

16831-CS®
®
®

In each set stainless steel funnels with lids are preassembled.

Accessories and Replacement Parts
Description

Pack Size

Order No.

Plug, conical, to close the venting hole beside the
3-way valve

106980225

Silicone O-ring for manifold female threads

36980235

Rubber tubing, 1 m

1

16623

142

Basic Filtration

Combisart® Manifolds

Glass Filter Holders; 30, 250 mL
For Particle Counting

Glass Filter Holders
Two compact vacuum filter holders for easy particulate ana­lysis. Both the top
and bottom part of the filter holders are easily and securely fastened together
using the metal clamp. The centering rim on the filter support ensures correct
positioning of the membrane filter. The glass frit filter support guarantees uniform distribution of retained particles on the filter surface.

Ordering Information
Description
Glass filter holder
Filter ∅Filtration area
Capacity
OutletGlass filter holder
Filter ∅Filtration area
Capacity
OutletOrder No.
30 mL
25 mm (or 24 mm)
Prefilter, 20 mm
3 cm2
30 mL
12 mm outer ∅16306

250 mL
47 mm (or 50 mm)
Prefilter, 40 mm
12.5 cm2
250 mL
15 mm outer ∅16307

Adapter, 16836 | Adapter, 16837
For use of a glass filter holder, 16306 or 16307, on a Combisart® stainless steel
manifold.

Ordering Information
Description

Order No.

 dapter with 11 mm opening in stopper;A
for using filter holder 16306 on a Combisart® manifold

16836

Replacement stopper for 16836

00280

 dapter with 14 mm opening in stopper;A
for using filter holder 16307 on a Combisart® manifold

16837

Replacement stopper for 16837

00281

Basic Filtration

Combisart® Manifolds

143

Polycarbonate Filter Holders
For Particle Counting

Polycarbonate Filter Holder, 250 mLThis reusable, practical filter holder made of autoclavable plastic is ideal for
analytical testing outside the laboratory. For use with 47 mm membrane filters.
Outlet: TR 20 × 2 mm male thread

Ordering Information
Description

Order No.

Polycarbonate filter holder without receiver flask

16511

Polycarbonate filter holder with receiver flask

16510

Hand vacuum pump with gauge and 60 cm PVC tubing

16673

144

Basic Filtration

Combisart® Manifolds

Ready-to-Use Biosart® 250 Funnels
For Particle Counting

250
200
150
100
40

Biosart® 250 Funnel
The Biosart® 250 Funnel has been specially designed for analytical quality
assurance. The sterile 250 mL plastic funnel guarantees fast filtration and highsample throughputs during routine testing. Its large inner ­diameter allows highflow rates, and the tapered inner walls permit thorough ­flushing of the funnel,
after filtration.

Ordering Information
Description

Order No.

Biosart 250 Funnel, 50 units, sterile-packaged

16407-25-ALK®

Single Support, 16840
For adapting a Biosart® 250 Funnel for use on a Combisart® stainless steel
manifold.

Ordering Information
Description

Order No.

Stainless steel filter support for stainless steel manifold.

16840

Replacement Parts
Description

Order No.

Stainless steel frit for 50 mm membrane filters

6980102

Stainless steel frit for 47 mm membrane filters

6980103

Silicone flat gasket underneath the frit6980124

PTFE flat gasket underneath the frit6980104

Silicone O-ring for 16840 male thread

6980274

145

Germany
Sartorius Lab Instruments GmbH & Co. KG
Otto-Brenner-Strasse 20
37079 Goettingen
Phone +49 551 308 0

For further information, visit
www.sartorius.com

Specifications subject to change without notice.
Copyright Sartorius Lab Instruments GmbH & Co. KG.
Version 08 | 2021

USA
Sartorius Corporation
565 Johnson Avenue
Bohemia, NY 11716
Phone +1 631 254 4249
Toll-free +1 800 635 2906