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Corynebacterium glutamicumῌ῍῎῏ῌ    ῍      ῎      ῏Ih5/@? #7Ic30>@) C. glutamicum  !"#$% &'() *+,-

YBxyOIz*Ea/0>$% &'yW3045) !"67# 4 $89%"%) :/0% &'{| I-. ¡jk}~" /) :9%( ;&<'=0>$? @,-(A BC)/@ MTG Oj¢LB/@? :£) C. gluta-./012)*7(D$E(./0FG+,@HI-..3micum t1.d0 MTG E 5 Oj#$r¤$% &'J/02D$? :@H) :1,K2ILLM ) C. glutamicum ¥S7D$ C. ammoniagenes Y

BC% &'"N34I1,#$56() M3M3781

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TUV ;WIU5) T37I<=X) Bacillus >YX)/) t-x¨ "eK#$I©S@? /L/ EB) MTGZ[) ?) @AYB \" /@% &'1,]E

(it(I 30>$@H) ª«. $T30^@? /L/ EB) T3B]">0J>_N3]7 1,() H7(C21,#$T.E`D % &'J/02Ea/0>/0> >?

M3M3E/0^@ C. glutamicum %&'Oj$? M3M3(TUV FGH"I/7Jbc#d2) J/1,]I4>0() E% &'1,].¬/) ./>% &'1,]"eK/) ,-6) D$>(FGLI0ui­®fg#$T."M4I"hS0^@?

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(2) C. glutamicum XY9I(% &'ObOREvSrlsm1,R"P9w$M47) TU1VG) 1x¢ 3C) % &'Oj]7FG. S0>$UG) yWG) XYZ \Iz#$/02{[4\|E]BV ) Ojw@M4% &'ObETB >? T30^@? ^) }4_`LB|$.) rlsm1,u~)3(¤S% &'"Oj1,#$V«7I.5M=rlsmab7D$% &'1,Iz#$c. S0>$?

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glutamicum EJS0>$) %&'. $rlsm

>de) TX" /rlsmt-1,"/0^@I4=$fgLB) TXIhM/) Cit./0 30>$H(3) Oj3@% &'( e"/0>$? T37IOjw@% &'() .\En%&'.qOR"/0>@? I) o dµ (IGF-1) "(qH.#$¶mn%&'

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ARrx+sh \"^T#' ./0) itOt712 30>$? @7() itOtu~7 <QRJ1q0^@T.LB) BIN34 1,56Eu30>@? /L/ EB) T37I\B30>@E% &'1,]">0J) h1,Z"v#$UV N3C> MTG 1,56"eK#$T.(
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y 14¹§ºq¦»y¼½#¨2 7r¶%$5¤29 CORYNEXῌ·3  !"#$ %
(4) C. glutamicum  50

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09' F5 CHO `?cd~e"2y<=>9':3 y"f2Qg( ?: @23 C. glutamicum 

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78 D] 0^9297" Uyz7{/ @u2' |85K9' ))78 :25_VD9;<!x%$ 5 0

Z"2 =>`?@AB;aCmUVH 053%A}~ H)2

Twin-aarginine
1D5Y8E Tat bF (T9' )) B%l)29translocation pathway) "cd9G2b0kD978_V;<F L>' 9 bF Sec bF"cNEXῌkD9 &GH=S3'

53d"L> CORY n d `?H5IJCKeL .9"%F5HUyz*y} /2 lH fMNgMO5`?P Qh2

z7{I>97 Z@29'PQhaCKeL& 9' 2?2%: G;i
Tat bF `?H5CKeL& ( B C. glutamicum  0

MO5`?PK 0"23% :

Qh29 O- 3P' B`?PQhjek2R9 a:%9V ¡¢@ £2  3%l )294 ST Sec bFm 0"2T0%"23 ))

UVH 0%?S  Tat bF D¤ 9"mUVH 05395%?"L>'"L>9'2¥¦4§¨539B43C. glutamicum N9_W n 1 Tat bF5YD9" ;i20QIZ@

oXY_W gp[2'micum n Tat bF C. gluta-0\]C4 F5 n v%wxH^k ¡ k©loB"ª?S" «2 ¬­oi©l$ ® 2 \FD' FUX45KSH?k2 ./q

¯a%F2 ¬i3F2¦*J(%rs5U ^ D9"539'

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