PD-1 Pathway
Recombinant Protein Collectionwww.acrobiosystems.com

ContentI.

Introduction

II. Biotinylated PD-1 Pathway Proteins

IIa. ELISA
IIb. FAC Sorting
IIc. Biopanning
III. HPLC-verified ProteinsIV. Full-length PD-1 Protein
V. FITC-labeled PD-1
VI. Full Product List

PD-1
PD-L1

B7-H2
PD-L2

CTLA-4
B7-1

B7-H5

Introduction
Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized
checkpoint proteins. The binding between PD-1 and its ligand PD-L1 and/or PD-L2 suppresses T-cell activation,
and allows cancer cells to escape from body’s immune surveillance. This mechanism prompts the idea thatneutralizing PD-1 pathway may help re-engage the immune system to fight against cancer.
In recent years, multiple lines of anti-PD-1 drugs have been developed, and have demonstrated enormous
potential in recent clinical trials. Patients with melanoma, renal cell carcinoma, non-small cell lung cancer(NSCLC), or hematological cancers all respond positively to this treatment.
ACROBiosystems provides a comprehensive series of PD-1 related products for researchers to investigate andtarget PD-1 pathway.

ELISA
ELISA is an assay technique routinely used tocharacterize protein-protein interaction. It’s often
used in initial high-throughput screening (HTS) of
compound library.
ACROBiosystems has developed a range of prebiotinylated PD-1 pathway proteins. Biotinylated
proteins can serve as two-way antibodies for both
capture and detection in ELISA assays. They
demonstrate higher specificity and detection
sensitivity than traditional antibodies.
Immobilized human PD-1 (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well)
can bind to biotinylated human PD-L1, His & Fc tag (Cat. No. PD1-H82F3)
with a linear range of 0.01-0.25 μg/ml.

Molecule Cat. No.
PD-1

PD1-H82F2

Product Desc.

Species

Biotinylated Human PD-1

Structure

PuritySize

PD-1 (25-167) Fc Avi His>95% SDS

25ug, 200ug

PD-1 (25-167) Fc Avi>92% SDS

25ug, 200ug

PD-L1 (19-238) Fc Avi His>95% SDS

25ug, 200ug

PD-L1 (19-238) Fc Avi>95% SDS

25ug, 200ug

Human
PD-1

PD1-M82F4

Biotinylated Mouse PD-1
Mouse

PD-L1

PD1-H82F3

Biotinylated Human PD-L1
Human

PD-L1

PD1-M82F5

Biotinylated Mouse PD-L1
Mouse

Suggested Pairs

Pair 1
Recommended for：Coat Human PD-L1
Detect Human PD-1

Pair 2

Product Desc.

Cat. No.

Species

Structure

Human PD-L1 / B7-H1 Protein, Fc Tag, lowendotoxin

PD1-H5258

Human

PD-L1 (19-238)

Fc

Biotinylated Human PD-1 / PDCD1, His Tag& Fc Tag, Avi Tag (AviTagTM)

PD1-H82F2

Human

PD-1 (25-167)

Fc Avi His

Mouse PD-L1 / B7-H1 Protein, Fc Tag

PD1-M5251

Mouse

PD-L1 (19-238)

Fc

Recommended for：Coat Mouse PD-L1
Detect Mouse PD-1

Biotinylated Mouse PD-1 / PDCD1, Fc Tag,Avi Tag (AviTagTM)

PD1-M82F4

Mouse

PD-1 (25-167)

Fc Avi

Pair 3

Human PD-1 / PDCD1 Protein, Fc Tag (HPLCverified), low endotoxin

PD1-H5257

Human

PD-1 (25-167)

Fc

Biotinylated Human PD-L1 / B7-H1, His Tag& Fc Tag, Avi Tag (AviTagTM)

PD1-H82F3

Human

PD-L1 (19-238)

Fc Avi His

Mouse PD-1 / PDCD1 Protein, Fc Tag

PD1-M5259

Mouse

PD-1 (25-167)

Fc

Biotinylated Mouse PD-L1 / B7-H1, Fc Tag,Avi Tag (AviTagTM)

PD1-M82F5

Mouse

PD-L1 (19-238)

Fc Avi

Recommended for：Coat Human PD-L1
Detect Human PD-1

Pair 4
Recommended for：Coat Mouse PD-L1
Detect Mouse PD-1

To further simplify your research, we’ve developed a PD-1[Biotin]: PD-L1 Inhibitor Screening Kit (Cat. No. EP101). This assay employs a simple colorimetric ELISA platform, which measures the binding between
immobilized human PD-L1 and in-house developed biotinylated PD-1 protein. This product is uniquely suitable
for rapid and high-throughput screening of putative PD-1 and PD-L1 inhibitors.

Principle of PD-1[Biotin]: PD-L1 Inhibitor Screening Kit

MoleculeCat. No.

Product Desc.

Species

PD-1 & PD-L1

EP-101PD-1[Biotin]: PD-L1 Inhibitor Screening Pair

Size
96 tests, 480 tests

Human

Immobilized PD-L1 at 2 μg/mL (100 μL/well) can bindbiotinylated human PD-1 with a linear range of 0.038 - 0.6
μg/mL when detected by Streptavidin-HRP.

Inhibition of PD-1-PD-L1 binding by an anti-PD-1 neutralizing
antibody is measured by the PD-1 [Biotinylated] : PD-L1
Inhibitor Screening ELISA Assay Pair (Cat. No. EP-101).

FAC Sorting
Biotinylated proteins can be used along withfluorophore-tagged SA to detect/isolate cells
expressing particular surface markers. In a newly
published US patent titled "ANTI-PD-1 ANTIBODIESAND METHODS OF USE THEREOF" (US patent
20160159905), the authors (from Pfizer Inc.) used
ACROBiosystems' biotinylated PD-L1 (Cat. No.
PD1-H82F3) and biotinylated PD-L2 (Cat. No. PD2H82F6) to compete with candidate antibodies in abinding assay against PD-1 expressing cells. This
assay provides measurement for the binding
activity of the corresponding antibodies.

Biotinylated PD-L1 and PD-L2 in FAC sorting

Biopanning
Biopanning has gradually become the mainstream approach for antibody development. The AviTagTM
biotinylated proteins can be used with SA-coated magnetic beads/surface in biopanning. It’s a much better
choice than non-biotinylated, or chemically biotinylated proteins because it offers higher coating density anduniformed antigen presentation.
Please note that the Fc tag is generally undesired for these experiments because it generates false positive
results. We have developed AviTagTM PD-1 pathway proteins with only His tag attached. These products arerecommended specifically for biopanning.

Molecule Cat. No.
PD-1

PD1-H82E4

Product Desc.

Species

Biotinylated Human PD-1

Structure

PuritySize

PD-1 (25-167)

Avi His>95% SDS

25ug, 200ug

PD-L1 (19-238)

Avi His>95% SDS

25ug, 200ug

PD-L2 (20-219)

Avi His>95% SDS

25ug, 200ug

Human
PD-L1

PD1-H82E5

Biotinylated Human PD-L1
Human

PD-L2

PD2-H82E8

Biotinylated Human PD-L2
Human

Biopanning Workflow with Biotinylated ProteinsHPLC-verified Proteins
The quality of recombinant proteins is essential in pharmaceutical assay development. In many situations,
SDS-PAGE alone is not sufficient for quality control purposes. To address this need, ACROBiosystems hasdeveloped a collection of HPLC-verified PD-1 and PD-L1 proteins.

The purity of Human PD-L1 Protein, mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) was more than
90% as determined by SEC-HPLC.

Molecule Cat. No. Product Desc.
PD-1

PD1-H5221

Species

Human PD-1

Structure

PD1-H5257

Human PD-1

His

>95% SDS>90% SEC-HPLC

100ug, 1mg

PD-1 (25-167)

Fc>95% SDS>90% SEC-HPLC

100ug, 1mg

PD-L1 (19-238)

His>98% SDS>90% SEC-HPLC

100ug, 1mg

PD-L1 (19-238) mFc>92% SDS>90% SEC-HPLC

100ug, 1mg

PD-L2 (20-219)>95% SDS>90% SEC-HPLC

100ug, 1mg

Human
PD-L1

PD1-H5229

Human PD-L1
Human

PD-L1

PD1-H52A3

Human PD-L1
Human

PD-L2

PD2-H5251

Human PD-L2
Human

Size

PD-1 (25-167)
Human

PD-1

PurityFcFull-length PD-1 Protein
ACROBiosystems newly added the world's first full-length human PD-1 protein. The full-length protein
demonstrates a much higher binding affinity against PD-L1 than the PD-1 ECD protein.

Molecule Cat. No.
PD-1

PD1-HC214

Product Desc.

Species

Full-length Human PD-1, Tag free

Structure

PuritySize

PD-1 (25-288 )>92% SDS

20ug

Human

Human PD-1, Full Length, Tag Free

Human PD-1, ECD, His Tag

Extracellular domain (25-170)

Intracellular domain(192-288)

Transmembrane domain (171191)

His TagProtein structures of full-length human PD-1 (Cat. No. PD1-HC214) andhuman PD-1 ECD protein (Cat. No. PD1-H5221).

Immobilized full-length human PD-1 (Cat. No. PD1-HC214, 20 ng/well) can bindhuman PD-L1 (Cat. No. PD1-H52A3) with a linear range of 1.2-78 ng/ml, which
is much higher than the PD-1 ECD protein (linear range 250 ng-1,250 ng/ml).

FITC-labeled PD-1
Biotinylated PD-1 can be used with fluorescence-labeled streptavidin in FACS and immunostaining
experiments. However, this process involves multiple rounds of incubation and washes, making it timeconsuming. ACROBiosystems offers an FITC-labeled PD-1 protein, which allows one-step fluorescence
detection.

Molecule Cat. No.
PD-1

PD1-HF257

Product Desc.

Species

FITC-labeled Human PD-1

StructurePD-1 (25-167)

Fc

PuritySize>95% SDS

25ug,200ug

Human

Immobilized Human PD-L1, Fc tag (Cat. No. PD1-H5258) at 10 μg/mL (100μl/well) can bind FITC-labeled Human PD-1, Fc tag (Cat. No. PD1-HF257) with alinear range of 0.3-5 μg/mL.

Full Product List
Molecule Cat. No. Product Desc.
PD-1

PD1-H82E4

Species

Biotinylated Human PD-1

Structure

PuritySize

PD-1 (25-167)

Avi His>95% SDS

25ug, 200ug

PD-1 (25-167)

Fc Avi His>95% SDS

25ug, 200ug>92% SDS

20ug

Human
PD-1

PD1-H82F2

Biotinylated Human PD-1
Human

PD-1

PD1-HC214

PD-1 (25-288 )Human PD-1, Tag Free, Full Length
Human

PD-1

PD1-H5221

Human PD-1

PD-1 (25-167)

His>95% SDS>90% SEC-HPLC

100ug, 1mg

PD-1 (25-167)

Fc>95% SDS>90% SEC-HPLC

100ug, 1mg

PD-1 (25-167)

Twin-Strep>95% SDS

25ug, 250ug

PD-1 (25-167)

Fc>95% SDS

25ug, 200ug

PD-1 (25-167)

Fc Avi>92% SDS

25ug, 200ug

PD-1 (25-167)

His>95% SDS

100ug, 1mg

PD-1 (25-167)

Fc>92% SDS

100ug, 1mg

PD-1 (25-167)

Fc>92% SDS

25ug, 200ug

PD-1 (21- 167) mFc>95% SDS

100ug, 1mg

PD-1 (25-167)

Fc>95% SDS

100ug, 1mg

PD-1 (25-167)

His>95% SDS

100ug, 1mg

PD-1 (25-167)

Fc>95% SDS

100ug, 1mg

PD-1 (25-167)

His>95% SDS

100ug, 1mg

Human
PD-1

PD1-H5257

Human PD-1
Human

PD-1

PD1-H5284

Human PD-1
Human

PD-1

PD1-HF257

FITC-labeled Human PD-1
Human

PD-1

PD1-M82F4 Biotinylated Mouse PD-1
Mouse

PD-1

PD1-M5228 Mouse PD-1
Mouse

PD-1

PD1-M5259 Mouse PD-1
Mouse

PD-1

PD1-M8259 Biotinylated Mouse PD-1
Mouse

PD-1

PD1-M52A4 Mouse PD-1
Mouse

PD-1

PD1-R5253

Rat PD-1
Rat

PD-1

PD1-R52H2

Rat PD-1
Rat

PD-1

PD1-RB5251 Rabbit PD-1
RabbitPD-1

PD1-R52H0

Rabbit PD-1
RabbitMolecule Cat. No. Product Desc.
PD-1

PD1-C5223

Species

Cynomolgus PD-1

Structure

PuritySize

PD-1 (25-167)

His>95% SDS

50ug, 1mg

PD-1 (25-167)

Fc>95% SDS

100ug, 1mg

PD-L1 (19-238)

Avi His>95% SDS

25ug, 200ug

PD-L1 (19-238)

Fc Avi His>95% SDS

25ug, 200ug

PD-L1 (19-238)

His>95% SDS

5ug, 20ug

PD-L1 (19-238)

His>98% SDS>90% SEC-HPLC

100ug, 1mg

PD-L1 (19-238) mFc>92% SDS>90% SEC-HPLC

100ug, 1mg

Cynomolgus
PD-1

PD1-C5254

Cynomolgus PD-1
Cynomolgus

PD-L1

PD1-H82E5

Biotinylated Human PD-L1
Human

PD-L1

PD1-H82F3

Biotinylated Human PD-L1
Human

PD-L1

PD1-H9229

C13 and N15-labeled, Human PD-L1
Human

PD-L1

PD1-H5229

Human PD-L1
Human

PD-L1

PD1-H52A3

Human PD-L1
Human

PD-L1

PD1-H5258

Human PD-L1

PD-L1 (19-238)

Fc>95% SDS

100ug, 1mg

PD-L1 (19-238)

Twin-Strep>95% SDS

25ug, 250ug

PD-L1 (19-238)

His>95% SDS

100ug, 1mg

PD-L1 (19-238)

Fc Avi>95% SDS

25ug, 200ug

PD-L1 (19-238)

Fc>95% SDS

200ug, 1mg

PD-L1 (19-238) mFc>95% SDS

100ug, 1mg

PD-L1 (19-238)

Fc>90% SDS

100ug, 1mg

PD-L1 (19-238)

His>95% SDS

100ug, 1mg

PD-L2 (20-219)

Fc>95% SDS>90% SEC-HPLC

100ug, 1mg

Human
PD-L1

PD1-H5282

Human PD-L1
Human

PD-L1

PD1-M5220 Mouse PD-L1
Mouse

PD-L1

PD1-M82F5 Biotinylated Mouse PD-L1
Mouse

PD-L1

PD1-M5251 Mouse PD-L1
Mouse

PD-L1

PD1-M52A2 Mouse PD-L1
Mouse

PD-L1

PD1-C5253

Cynomolgus PD-L1
Cynomolgus

PD-L1

PD1-C52H4

Cynomolgus PD-L1
Cynomolgus

PD-L2

PD2-H5251

Human PD-L2
Human

Molecule Cat. No. Product Desc.
PD-L2

PD2-H82E8

Species

Biotinylated Human PD-L2

Structure

PuritySize

PD-L2 (20-219)

Avi His>95% SDS

25ug, 200ug

PD-L2 (20-219)

His>95% SDS

100ug, 1mg

PD-L2 (20-219) mFc>95% SDS

100ug, 1mg

PD-L2 (20-219) mFc>95% SDS

100ug, 1mg

PD-L2 (20-219)

Fc>95% SDS

100ug, 1mg

PD-L2 (20-219)

His>95% SDS

100ug, 1mg

Human
PD-L2

PD2-H5220

Human PD-L2
Human

PD-L2

PD2-H52A5

Human PD-L2
Human

PD-L2

PD2-M52A5 Mouse PD-L2
Mouse

PD-L2

PD2-M5254 Mouse PD-L2 / B7-DC
Mouse

PD-L2

PD2-M52E3 Mouse PD-L2
Mouse

PD-1 & PD-L1

EP-101PD-1[Biotin]: PD-L1 Inhibitor
Screening Pair

96tests, 480tests
Human

B7-H5
PD-L2
PD-1
B7-H2

PD-L1

B7-1

CTLA-4

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PD1-EN02E01-OPE-2017-07-19