Workflow Guide

freeslate

Biologics formulation
Freeslate takes on your entire biologics formulation development process and automates it end-to-end. It’ll tackle
buffer prep, buffer exchange, pH checks, sample prep andanalysis. Get your accelerated stability studies done withintegrated sample incubation and automated freeze/thaw
systems. Do it all using a single, totally configurable platform and crank through more candidates and formulations
than ever.

Applications• Rapidly characterize a wide range of protein formulations
with limited material

freeslate configured for biologics formulation• Perform developability and preformulation screens• Evaluate formulation robustness• Easily implement DOE to find your optimal formulation• Prepare and analyze formulations for pH, viscosity,turbidity and visible particles• Perform automated agitation and temperature stressstudies• Manage and track formulations and analytical results tofacilitate rapid scientific decisions

Key features

Visual inspection
stationMulti-channel pH
probesViscosity station• Versatile liquid handling for viscous and non-viscous
solutions
1• Compatible with a wide range of plates and vials• Automated plate-based buffer exchange• Automated protein stressing — heat, cool, stir and shake2

formulations

4• On-deck visible particle, turbidity and color analysis• High-throughput pH and viscosity measurements

35678• Low-bioburden enclosures with HEPA filters

91011

12

Example freeslate for biologics formulation deck layout
12
34
5
6

4-tip pH probe
Volume check
Buffer exchange module
Viscosity station
Visual inspection station
Vial capping and decappingstation

7 Vortexing station
8 Cooled storage bay
9 Heating/stirring station
10 Tip/rack holder
11 Wash station
12 Tip/rack holder

Workflow GuideAvailable options
pH measurement

Visual inspection station (VIS) analyses

Configuration: Single or 4-channel probe
Measurement time per 96-well plate: ~ 34 minutes
Range: pH 1–13
Resolution: 0.01 pH units
Repeatability: ±0.1 pH units

Includes:• Visual particle analysis• Turbidity• Color measurement

Multi-channel liquid dispenser
6-channel variable volume liquid dispense
Disposable tips: For no washing, no sample carry-overAutomated variable pitch: For reformatting between plates
and vials

Viscous liquid dispense
Technology: Positive Displacement Tip (PDT)
Disposable tips: 10 µL to 10,000 µL from Eppendorf and Rainin
Viscosity: 1 cP to 1,000 cP

Vial/plate gripper
Plate size: Standard microtiter
Vial size: 1–125 mL
Total mass: Up to 3 kg

Vortexing station
Orbital: Up to 2000 rpm max
Maximum vortexing mass: 860 g

Heating/cooling/stirring station
Temperature range: -20–180 °C
Mixing: Up to 750 rpm
Mixing type: Magnetic tumble stirring

Rack/plate carousel• Additional storage for samples, solutions, buffers and tips
• Plate transfer between robotic systems enabling
full integration

Viscosity station
Measurement range: 2–100 cP
Accuracy: ±0.5 cP + 10% of the actual viscosity
Repeatability: StDev <0.5 cP + 5% of mean
Sample volume: 100 μL
Minimum volume in well: 200 μL
Temperature range: 4–40 °C
Temperature accuracy: ±1 °C
Measurement time: 6 min/sample
Throughput: 10 samples/h

Vial capping/de-capping station
Vial range: 2 mL to 125 mL

Vial size: 2–20 mL
Recommended sample volume: 1 mL in 2 mL serum vial
Measurement time: 2–3 min per vial

Suspended visible particle detection
Minimum particle size detected: 80 μm
Maximum solution viscosity: Dependent on vial configuration• 2 mL vial: 30 cP• 20 mL vial: 35 cP
Particle count accuracy:• No particles: 0 particles detected• 1–3 particles: Detect at least 1 particle• 4–9 particles: Actual particle count ±2 particles• 10–25 particles: Actual particle count ±5 particles

Turbidity
Measurement range: 10–1,000 NTU
Measurement accuracy: ±5 NTU (for non-absorbing samples)
Repeatability: ≤3 NTU for 10 consecutive samples

Color measurement
Color: Correct match of Euro Pharmacopeia BY1–BY7 standards

Buffer exchange
Compatibility: Ultrafiltration microtiter plates optimized forMillipore’s MultiScreen Ultracell plates
Pressure range: 0–60 psig
Vortexing: Up to 1600 rpm; speed and duration fully
programmableOn-deck third-party instrument physical integration• DLS• UV-Vis plate reader• Centrifuge• Plate sealer• Shaking incubator • Incubators• Freeze/thaw blocks
Other systems are available for on-deck integrations.
Please contact Unchained Labs for a full list of systems.

Off-deck third-party instrument virtual integration• HPLC• cIEF
Other systems available for virtual integration.
Please contact Unchained Labs for a full list of systems.

Facilities requirements
Physical: 243.3 cm W x 152.4 cm D x 257.1 cm H, 647 kg
Electrical:
freeslate:
208–230 V ±10 %, 50–60 Hz, 16–20 A
Computer:
US: 115 V ±10 %, 60 Hz, 10 A
EU: 220–230 V ±10 %, 50 Hz, 16 A
Compressed dry air: 0.5–0.9 MPa (70–130 PSI), 4L/min

Unchained Labs
6940 Koll Center Pkwy, Suite 200
Pleasanton, CA 94566
Phone: 1.925.587.9800
Toll-free: 1.800.815.6384
Email: info@unchainedlabs.com© 2016 Unchained Labs. All rights reserved. Freeslate is a trademark
and Unchained Labs is a registered trademark of Unchained Labs.
All other brands or product names mentioned are trademarks
owned by their respective organizations.
Rev B