Fc Receptor Family Proteins
The efficacy of a therapeutic antibody not only depends on the Fab fragment and its binding activity to the targetantigen, but also depends on the Fc fragment and its interaction with key Fc receptors. The binding affinity of the Fc
fragment towards FcRn (FCGRT&B2M) would predict the antibody’s half life, while that between the Fc fragment andFCGRIIIA (CD16a) would influence the antibody’s ability to elicit ADCC. Hence, screening for desired binding affinity tothese Fc receptors is an essential component in the development of a therapeutic mAb.
ACROBiosystems offers a comprehensive collection of recombinant Fc receptor proteins, including their commonvariants, to help expedite your mAb development. These products have the following features that make them uniquely
suitable for pharmaceutical research.

Biotinylated Proteins Available
The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use
biotinylated Fc receptors (marked in red in the table below). These proteins are produced using our in-house developedlabeling technique, which confers high bioactivity and minimal batch-to-batch variation.

Molecule

FcRnCat. No.

Species

Strep IIFCGRT (24-297)

His

B2M (21-119)

Strep IIFCGRT (24-297)

His

B2M (21-119)

Strep IIFCGRT (23-298)

His

B2M (21-119)

Strep IIFCGRT (24-297)

Avi

B2M (21-119)

Strep II

25ug, 200ug

FCM-C5284

50ug, 1mg

FCM-R5287

50ug, 1mg
Rat

FcRn
(Biotin-labeled)

B2M (21-119)

FCM-H8286

Cynomolgus / Rhesus
FcRn

His50ug, 1mg

Human
FcRn

FCGRT (24-297)

Size

FCM-H5286
Human

FcRn
(Biotin-labeled)

Structure

FCM-H82W4

His25ug, 200ug

HumanFc Receptor Family Proteins
MoleculeCat. No.

FcRn

FCM-M52W2

Species

StructureSize

FCGRT (22-297)

His

B2M (21-119)

Twin-StrepFCGRT (22-297)

Avi

B2M (21-119)

Twin-StrepFCGRT (24-297)

Avi

B2M (21-119)

Strep IIFCGRT (16-289)

His

B2M (21-118)

Strep IIFCGRT (23-298)

Avi

B2M (21-119)

Strep II

50ug, 1mg
Mouse

FcRn
(Biotin-labeled)

FCM-M82W6
Mouse

FcRn
(Biotin-labeled)

His25ug, 200ug

FCM-P5280

50ug, 1mgPorcine

FcRn(Biotin-labeled)

25ug, 200ug

FCM-C82W5
Cynomolgus / Rhesus

FcRn

His

His

FCM-R82W725ug, 200ug
Rat
F176

Fc gamma RIIIA

CD16a (17-208)

CDA-H5220

His100ug, 1mg

His100ug, 1mg

Human
V176

Fc gamma RIIIA

CD16a (17-208)

CD8-H52H4
Human

F176

Fc gamma RIIIA(Biotin-labeled)

Avi

His

25ug, 200ug

CD16a (17-208)

Avi

His

25ug, 200ug

CD16a (32-215)

His

100ug, 1mg

CD16b (17-200)His

100ug, 1mg

CD16b (17-200)

His100ug, 1mg

CD16a (17-208)

CDA-H82E8
HumanV176

Fc gamma RIIIA(Biotin-labeled)

CDA-H82E9
Human

Fc gamma RIIIA

CDA-M52H8
Mouse

Fc gamma RIIIB

CDB-H5222
Human

Fc gamma RIIIB

CDB-H5227
Human

Fc Receptor Family Proteins
MoleculeCat. No.

Fc gamma RIIlB(Biotin-labeled)

CDB-H82E4

Species

Structure

CD16b (17-200)

Size

His

Avi

25ug, 200ug

Human
H167

Fc gamma RIIA

CD1-H5223

CD32a (36-218)

His

100ug, 1mg

His

100ug, 1mg

Human
R167

Fc gamma RIIA

CDA-H5221

CD32a (36-218)
Human
H167

Fc gamma RIIA
(Biotin-labeled)

Avi

His25ug, 200ug

CD32a (36-218)

Avi

His

25ug, 200ug

CD32b (46-217)

His

CD32b (46-217)

Avi

CD32b (40-217)

His

100ug, 1mg

CD64 (16-288)

His

100ug, 1mg

CD64 (25-297)

Avi

CD64 (25-297)

His

100ug, 1mg

Avi

CD23 (48-321)

25ug, 200ug

CD32a (36-218)

CDA-H82E6
Human

R167

Fc gamma RIIA
(Biotin-labeled)

CDA-H82E7
Human

Fc gamma RIIBCDB-H5228

100ug, 1mg

Human
Fc gamma RIIB
(Biotin-labeled)

CDB-H82E0

His

25ug, 200ug

Human
Fc gamma RIIBCDB-M52H7
Mouse

Fc gamma RIFCA-H52H2
Human

Fc gamma RI(Biotin-labeled)CD4-M52E8

His25ug, 200ug

Mouse
Fc gamma RI

CD4-M5227
Mouse

CD23(Biotin-labeled)CD3-H82Q5

His
Human

Fc Receptor Family Proteins
Human Cell-expressed

Binding Activity Validated

All of our Fc receptor proteins are produced in
HEK293 cells, which ensures the authentic posttranslational modification essential for their binding
activities.

The binding activity of our Fc receptors has beenthoroughly evaluated by both SPR and ELISA analyses
prior to launch.

High Purity & High Quality
To meet the high purity requirement ofpharmaceutical applications, most of our proteins
have to go through both SDS-PAGE and HPLC
analyses. Only those meeting all requirements will
be issued a lot-specific certificate of assurance andbe released.

The purity of human
FcRn (Cat. No. FCMH5286) is greater than
95% as determined in a
HPLC analysis.

Immobilized
Herceptin(Human IgG1) at 5 μg/mL(100 μL/well) can bindhuman FcRn (Cat. No.
FCM-H5286) with a linear
range of 0.2-5 μg/ml as
determined in a functionalELISA.

Immobilized Human FCGR3A /CD16a (V176), His Tag (Cat. No.
CD8-H52H4) can bind Ipilimumabwith affinity constant around 171
nM in a SPR assay (Biacore 2000).
The data is generally provided by
Biaffin GmbH &Co KG, Germany.

+ 1 800-810-0816 (US/Canada)+ 86 400-682-2521 (Asia & Pacific)
techsupport@acrobiosystems.com
www.acrobiosystems.com
1 Innovation Way,
Newark, DE 19711
FCR-EN01E03-OPE-2017-07-07
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