SENP1 Antibody
Rabbit Polyclonal
Antigen Affinity Purified

Protein ID

NP_055369.1

Catalog No.

A302-928A

GeneID

29843

Lot No.

A302-928A-1

APPLICATIONSIP

SPECIES REACTIVITY

Human

PRESUMED REACTIVITY

Based on 100% sequence identity, this antibody is predicted to react with Orangutan

AMOUNT

100 µlCONCENTRATION

1000 µg/ml

STORAGE/SHELF LIFE

2 - 8° C / 1 year from date of receipt

PHYSICAL STATE

LiquidBUFFER

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

ISOTYPE

IgG

ORIGINUSA

PRODUCTIONPROCEDURES

Antibody was affinity purified using an epitope specific to SENP1 immobilized on solid support.
The epitope recognized by A302-928A maps to a region between residue 150 and 200 of human
SUMO1/Sentrin Specific Peptidase 1 using the numbering given in entry NP_055369.1 (GeneID29843).
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4
equals 1.0 mg of IgG.

APPLICATIONSCentrifuge tube to remove product from lid. Optimal working dilutions should be determined
experimentally by the investigator. Prepare working dilution immediately before use.
Western Blot

Not recommended

Immunoprecipitation

2 - 5 µg/mg lysate

APPLICATION NOTES

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020),
Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE(link to IP-western blot protocol in Additional Info section below).

ADDITIONAL INFO

https://www.bethyl.com/product/A302-928A
Use the link above to view SDS, a current list of citations, and other product specific information.
IP-western blot protocol: https://www.bethyl.com/content/protocol_IP_WB

This document certifies that this product has met all of the quality control standards defined by Bethyl Laboratories, Inc.
Eric McIntush, PhD | Chief Scientific Officer
Date: June 21, 2019

Bethyl Laboratories, Inc. • 25043 West FM 1097 • Montgomery, TX 77356 •800.338.9579 • 936.597.6111 • 866.597.6105 (FAX) • www.bethyl.com • technical@bethyl.com

1

For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.

SENP1 Antibody

A302-928A

Detection of human SENP1 by western blot ofimmunoprecipitates. Samples: Whole cell lysate (1 mg forIP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity
purified rabbit anti-SENP1 antibody A302-928A used for IP
at 3 µg/mg lysate. SENP1 was also immunoprecipitated byrabbit anti-SENP1 antibody A302-927A, which recognizes
an upstream epitope. For blotting immunoprecipitated
SENP1, A302-927A was used at 0.4 µg/ml,. Detection:
Chemiluminescence with an exposure time of 30 seconds.

Bethyl Laboratories, Inc. • 25043 West FM 1097 • Montgomery, TX 77356 •800.338.9579 • 936.597.6111 • 866.597.6105 (FAX) • www.bethyl.com • technical@bethyl.com

2For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.