Product Data SheetpBABE-Puro Retroviral Vector
CATALOG NUMBER:

RTV-001-PURO

STORAGE: -20ºC

QUANTITY AND CONCENTRATION: 10 µg at 0.5 µg/µL in TE
BackgroundRetroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Cell
Biolabs’ retrovirus vector is based on the pBABE vector system, which is derived from Moloney murine
leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing
elements, and a target gene. The viral env gene, produced by the package cell line, encodes the envelopeprotein, which determines the viral infectivity range. Transfection into a package cell line produces hightiter, replication-incompetent viruses. In addition to transfer and expression of exogenous genes inmammalian cells, recently, retroviruses are used to express silencing RNAs (siRNA) to decrease theexpression of target genes both in vitro and in vivo.
The vector contains the bacterial origin of replication, ampicillin-resistance gene, and puromycinresistance gene for the grwoth of infected mammalian cells to select stable cell lines (Figure 1).

Figure 1. pBABE-Puro Retroviral Vector Map

Safety Consideration
Remember that you will be working with samples containing infectious virus. Follow the recommended
NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips andwork under a biosafety hood.

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