Plasma-SeqSensei™ Sample Report

Sample Information
Sample ID

Analysis Date

DNA Input*

Test

Flowcell ID

Analysis ID

Lot Number

MAF022021-09-30

7154 GEBC_RUO1H3KT7AFX3

210930 LAB

D21001*Genome Equivalents: number of amplifiable haploid genomic copies analyzed by the assay. 1 GE = 3.3 pg of DNA.
The DNA input is listed as not quantifiable if too much / not enough input DNA is present or due to failure of Positive Control.

Sample Validity
Run Validity

Sample Validity

Positive control:

No template control:

Sequencing depth:

Quantification:

For detailed information about validity parameters please refer to the QC part of this report.
Mutation calls will only be reported as valid, if all controls and sample performance criteria are met.
Otherwise, the mutation calls will be marked as invalid.

Sample Mutation Status
AKT1ERBB2

ESR1

KRAS

PIK3CA

TP533

201218Analysis: 210930 LAB (2021-09-30)
Sample ID: MAF02

For research use only.
Not for use in diagnostic procedures.

Page (1/6)

Plasma-SeqSensei™ Sample Report

Somatic Mutations DetectedMAF

MutantMolecules

0.112%

80.234%170.103%70.124%90.136%100.356%25

COSV55873239

0.122%

9COSV55873195

0.265%

190.095%

70.104%70.089%60.089%60.094%70.097%

7

COSV52678014

0.106%

8p.R175H

COSV52661038

0.276%

20

p.C242Afs*5

COSV52760886

0.369%

26c.734G>A

p.G245DCOSV52667838

0.262%

19

ENST00000269305

c.743G>A

p.R248Q

COSV52661580

0.369%

26

ENST00000269305

c.818G>A

p.R273HCOSV52660980

0.386%

28TP53

ENST00000269305

c.827C>T

p.A276VCOSV52704969

0.274%

20

TP53

ENST00000269305

c.848G>A

p.R283HCOSV52700134

0.089%

6TP53

ENST00000269305

c.851C>A

p.T284K

0.089%

6TP53

ENST00000269305

c.925C>A

p.P309T

0.085%

6

TP53

ENST00000269305

c.925C>T

p.P309S

0.094%

7

TP53

ENST00000269305

c.984C>A

p.F328L

0.104%

7GeneID

Transcript

Coding DNA
ChangeAmino Acid
ChangeAKT1

ENST00000554581

c.47-25G>T

p.?

AKT1

ENST00000554581

c.49G>A

p.E17K

AKT1

ENST00000554581

c.69G>T

p.R23=ERBB2

ENST00000269571

c.
2313_2324dup

p.Y772_A775dup

ERBB2

ENST00000269571

c.2360G>A

p.G787DKRAS

ENST00000256078

c.35G>A

p.G12DCOSV55497369

PIK3CA

ENST00000263967

c.1633G>A

p.E545KPIK3CA

ENST00000263967

c.3140A>G

p.H1047R

TP53

ENST00000269305

c.1002G>A

p.G334=

TP53

ENST00000269305

c.1017G>T

p.E339D

TP53

ENST00000269305

c.328C>T

p.R110C

TP53

ENST00000269305

c.336C>A

p.G112=

TP53

ENST00000269305

c.372C>T

p.C124=

TP53

ENST00000269305c.469G>A

p.V157ICOSV52676009

TP53

ENST00000269305

c.482C>T

p.A161V

TP53

ENST00000269305

c.524G>ATP53

ENST00000269305

c.723delTP53

ENST00000269305

TP53
TP53

COSMIC ID

COSV62571334

COSV54062409

COSV52684761

COSV52729434

Validity• No germline mutation calls detected for this sample.
For a complete listing of mutation calls (including invalidated calls), please refer to the VCF output.

Analysis: 210930 LAB (2021-09-30)
Sample ID: MAF02

For research use only.
Not for use in diagnostic procedures.

Page (2/6)

Plasma-SeqSensei™ Sample Report

Sequencing Run Quality
Category

Acceptance Range

Value

Sequencer ID-/-NDX550262_RUO

Sequencing Kit-/-Reads Passing Filter≥ 80%

Cluster Density[Clusters / mm2]

NextSeq™ 500/550:≤ 220kPercentage >Q30

≥ 80%

Analysis: 210930 LAB (2021-09-30)
Sample ID: MAF02

MiSeq™:≤ 1400k

For research use only.
Not for use in diagnostic procedures.

Page (3/6)

Plasma-SeqSensei™ Sample Report

Sample Validity
Sample Coverage

Sequencing depth per target region: green bars indicate sufficient coverage while orange bars indicate insufficient target coverage.

Positive Control
LimitsResultDepth of coverage

1.0>1Quantification

500.0 GE<x GE<1500.0 GE

1410 GE

Detection

10.0≥10If the quantification of the positive control fails, mutant molecule values (MM) will not be reported per sample.

No Template Control
NTC ID

Avg. UID threshold

Avg. UID result

NTC

150.0

Analysis: 210930 LAB (2021-09-30)
Sample ID: MAF02

For research use only.
Not for use in diagnostic procedures.

Page (4/6)

Plasma-SeqSensei™ Sample ReportSample Metrics
Read Overview
Category

ResultReads imported from sequencer

14,896,267

Reads passing quality filter

14,769,485

Usable read rate

99.15%

Reads with sufficient UID size

14,311,470

Read rate with sufficient UID size

96.9%

Raw error corrected reads

818,829

Filtered error corrected reads

818,289

Filtered error corrected read rate

99.93%FASTQ reads are initially filtered by the sequencing device (Illumnina NextSeq™ 500\550 or Illumina MiSEQ™). For the PlasmaSeqSensei™ assay, these reads are further inspected for low quality nucleotide calls in general and for the bar-code sequence (UID/ UMI) in particular.
From this, the usable read rate is determined. Reads are grouped according to their bar-code sequence and bar-coded groups withat least 4 members are further processed (reads with sufficient UID size).
Error corrected reads are generated from these groups with a certain conversion rate.

Sequencing Quality

Distribution of QPhred quality values in error corrected reads. Shaded area indicates QPhred quality values excluded from mutation
calling. A subset of 10,000 reads is used per FASTQ file of the sample to generate this plot.

Analysis: 210930 LAB (2021-09-30)
Sample ID: MAF02

For research use only.
Not for use in diagnostic procedures.

Page (5/6)

Plasma-SeqSensei™ Sample Report
Analysis Software and Database Information
Analyzed by Plasma-SeqSensei™ Software v1.1.0
Assay configuration BC_RUO1
Sequencing result annotation supported by:• COSMIC Databse v92 (https://cancer.sanger.ac.uk/cosmic)• The Ensembl database
The Plasma-SeqSensei™ Software is for research use only. Not for use in diagnostics procedures.

Date, Signature

Analysis: 210930 LAB (2021-09-30)
Sample ID: MAF02

For research use only.
Not for use in diagnostic procedures.

Page (6/6)