Scientific Program17:00-

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17:30-17:35

Opening remarks (Prof. Daisuke TAKATA)First Session (S.TAIRA as chairman)17:35-17:55

Dr. ShannonCornett (Bruker Daltonik GmbH)'Imaging mass for Food Analysis'17:55-18:20

Prof. Dimitri Heintz (University of Strasbourg)'Plant Imaging Mass Spectrometry (PIMS) '18:20-18:35

Coffee Break

Second Session (K.YOSHINAGA as chairman)18:35-19:00

Prof. Naohiro Gotoh,(Tokyo University of Marine Science and Technology)'Imaging of administrated DHA and arachidonic acid in mouse brain'19:00-19:25

Prof. Laura Sanchez (University of Chicago Rockford)'Imaging mass spectrometry in plant microbial pathogens reveals chemicalcrosstalk '19:25-19:30 Closing address (Prof. Shu Taira)Simultaneous translator：He Min

'Imaging mass for Food Analysis'

Dr. Shannon CornettBruker Daltonics, USMALDI imaging provides a fast and reliable screening tool for direct analysis fromtissue and allows the histological mapping of compounds such as proteins, peptides,lipids and metabolites without the need for molecular probes. The technique is fullycompatible with conventional histological techniques and gives a unique molecularinsight in tissue sections. This 2nd and 3rd dimension data provides promising tools inthe exciting fields of biomarker evaluation in drug development and as a molecularvisualization tool for agricultural analyst.

Plant Imaging Mass Spectrometry (PIMS)Université de Strasbourg, France

Dimitri Heintz PhD(please sendit directly inthe email(JPEGformat)

Our team is specialized in the development of experimental design aimed to theidentification of small bioactive molecules coming from plants mainly but alsofrom animals, microbes and human. The identification of small molecules is made by means ofanalytical tools using chromatography, mass spectrometry and mass spectrometry imaging (MSI).
One of our major topic is “Environmental Metabolomics». The aim of that project is to understandhow plants cope with a polluted environment. The challenge is to be able to identify a huge amountof different micro-pollutants like pesticides, drugs, and many other toxics present in waste water,polluted soil or in plants that are able to make phytoremediation (elimination) of micro-pollutants.
Using MSI technic on poplar leaves we can determine the spatial and temporal distribution of micropollutants in the different plant tissues and better understand how the plant copes with pollution.

Head of the Plant Imaging Mass Spectrometry (PIMS) lab.
Specialized in the development of experimental design aimedto the identification of small bioactive molecules in plantsmainly but also in animals, microbes and human. PIMSis located in France in the city of Strasbourg atthe Institute of Plant Molecular Biology (IBMP)which is the biggest center of academic plant research in FranceEducation2000 : Master in Microbiology, University of Bordeaux (France)2004 : PhD, “Plant phosphoproteomics”, University of Freiburg (Germany)2007: Assistant professor & Head of PIMS at the IBMP (France)2012 : Research Habilitation (HDR), University of Strasbourg (France)Activities2005 : Postdoc Harvard medical School “Phospho-proteomics” (USA)2007 : Postdoc, company Sanofi-Aventis Pharma, (France)2007- present : Team Leader, PIMS, IBMP (France)2008- present: Lecturer, “Metabolomics” at the University of Strasbourg (France)

Scientific interestsMetabolomics, Lipidomics, & Mass spectrometry ImagingDevelopment of methodologies based on chromatography and mass spectrometry for theidentification of small bioactive molecules.

Imaging mass spectrometry in plant microbial pathogens reveals chemical crosstalkSanchez.LauraThe University of Illinois at ChicagoRalstonia solanacearum is a soil-borne plant pathogenic bacterium and has been associatedwith a number of plant- and soil-associated fungi. We sought to determine if R. solanacearumchemical communication directs symbiotic development of polymicrobial consortia. Usingimaging mass spectrometry, we identified an undescribed lipopeptide, ralsolamycin, producedby an R. solanacearum non-ribosomal peptide synthetase-polyketide synthase hybrid. We alsodiscovered in further screening that there is a conserved antagonistic communication betweenRalstonia solanacearum and plant-pathogenic fungi from disparate genera, Fusarium andBotrytis. Exposure of Fusarium fujikuroi to the bacterial lipopeptide ralsolamycin resulted inproduction of the antibacterial metabolite bikaverin specifically in fungal tissues invaded byRalstonia. Remarkably, ralsolamycin induction of bikaverin was conserved in a Botrytis cinereaisolate carrying a horizontally transferred bikaverin gene cluster. These results indicate thathorizontally transferred gene clusters may carry regulatory prompts that contribute to conservedfitness functions in polymicrobial environments.

BioDr. Sanchez was born and raised in Northern California. She attended Whitman College inWalla Walla, WA where she obtained a Bachelor of Arts degree in Chemistry (2007) and was adodgeball champion (05-07). Laura decided to pursue a Ph.D. in Chemistry at the University ofCalifornia, Santa Cruz in Prof. Roger Linington as an NSF graduate research fellow. In the Fallof 2012, she joined Prof. Pieter Dorrestein's lab at UC San Diego as an NIH IRACDA Fellow.
Since 2015, she has been in her independent position at UIC and her NIH and NSF fundedresearch program utilizes a variety of mass spectrometry techniques to probe how cells andmicrobes use chemistry to coordinate activities in a variety of biological systems.

‘Imaging of administrated DHA and arachidonic acid in mouse brain’Naohiro GotohTokyo University of Marine Science and Technology, JapanIt is well known that main components of brain lipids are docosahexaenoic acid(22:6n-3, DHA) and arachidonic acid (20:4n-6, AA). Mammals cannot synthesize thesefatty acids in the body and have to take them from foods. However, it is not well knownhow DHA and AA migrate in the body and accumulate in the brain. To resolve thismatter, we synthesized stable isotope (SI) labeled DHA and AA to distinguish fromnatural DHA and AA because their molecular are different from natural ones. Thesynthesized SI labeled DHA and AA were administrated to mouse and their distributionwas observed. The distribution of SI labeled DHA and AA in mouse brain wassuccessfully monitored with imaging mass spectrometry method (IMS). Furthermore,distribution of elongated fatty acid from AA, docosatetraenoic acid (22:4n-6), was alsoconfirmed. The combination of SI labeled fatty acid and IMS would become strong toolto reveal the role of DHA and AA in brain.