CRISPR gene knockout
Edit-R™ reagent selection guide

CRISPR based gene knockout requires two components: guide RNA and Cas9 nuclease. Horizon offers a diverse selection offormat options for both. The most appropriate formats will depend on your particular application and cell type. Use this guideto determine the best options for your experiment.

Guide RNA format selection

N ew

Synthetic
crRNA

Synthetic
sgRNA

LentiviralsgRNA*

Lentiviral Allin-one sgRNA*

Predesigned guide RNA guaranteed to edit
the target geneXXXXDNA-free - eliminates risk of DNA integration

X

X

Simplified editing - combine sgRNA + Cas9 nuclease
expression into a single vector

X

Recommended for difficult-to-transfect cells

X

X

Enrichment options

GFP

Delivery methods

DF

Single component guide format

DF

X

X

X

Predesigned guides for human and mouse genes

X

X

X

X

Custom designed guides available

X

X

X

X

Whole genome and gene family libraries available

X

X

X

Automation friendly for high throughput applications

X

X*Available as ready-to-use viral particles or renewable glycerol stocks.
GFP

GFP

ElectroporationDF

DharmaFECT transfection

Viral transduction

Puromycin

Edit-R guarantee
All formats of Edit-R predesigned guide RNA are fully guaranteed to edit the gene of interest. If the positive control works, but your experimental guide
does not, then Horizon will replace the guide with a different design at no cost.

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Cas9 nuclease format selection
Cas9 nuclease expression may be transient, constitutive, or even inducible.

Cas9protein

Cas9mRNA

DNA-free - eliminates risk of DNA integration

XX

Complex with synthetic guide RNA - no need to co-transfect

XLentiviralCas9 particles

Recommended for difficult-to-transfect cells

X

Inducible expression

X

Multiple promoter options

XCreates stable cell lines

X

Enrichment options
GFP

GFP

mKate2

mKate2GFP

GFP

mKate2Blasticidin

Edit-R knockout data
Functional protein knockouts in CD4+ T cells

Functional knockout of FUS in U2OS cells
Hoechst

FITC

CD28

Composite

CD3E

CXCR3

NTC

FUS wtHoechst

FITC

Composite

TargetIndividualsgRNAFUS KO

CD4
Lentiviral Cas9 was used to constitutively express Cas9 nuclease under the CAG
promoter and Edit-R pooled synthetic sgRNA was transfected using DharmaFECT
transfection reagent. Cells were then stained for FUS expression using FITC
conjugated antibody.

Primary human CD4+ T cells were nucleofected with Cas9 RNP using an individual
predesigned synthetic sgRNA per gene target. Functional knockout was assessed
as a percent of cells not expressing the target gene by FACS analysis.

Ready-to-go and customizable knockout cell lines
Focus on phenotype and leave the editing to us.
• Choose from over 7,500 ready-to-go HAP1 knockout models• Browse more than 300 Cancer related knockout and knock-in cell lines• Use our Custom Engineering service to generate a cell model specific to your application

CRISPR knockout for functional genomic screening
Screen your own knockout library or have the Horizon experts run a screen for you.
• Design and order a custom library of 20 or more genes with the Cherry-pick library tool
• Investigate entire gene families or biological pathways with predefined CRISPR libraries• Use our Functional Genomic Screening service and work with a dedicated team of experts for each screening project

Please contact Horizon or visit horizondiscovery.com for more informationt +44 (0) 1223 976 000 (UK) or +1 855 772 4252 (USA)f +44 (0)1223 655 581
e info@horizondiscovery.com
w www.horizondiscovery.com
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