The Journal of Poultry Science：Check List and Sample Manuscript
The Journal of Poultry Science

Manuscript Pre-edit Check List
To Authors
Please check your manuscript using the following items, and give marks in boxes to confirm it correct.
Sample manuscript is shown on the second and following pages.
Recent published papers: https://www.jstage.jst.go.jp/browse/jpsa/-char/en

Items
First page
Title, Name of Author(s), Affiliation and Address, Running Title, andCorresponding Author should be given.
The first and last names of each author should be written in full spelling. Middlename should be written as the initial.
Running title should be given within 6 words.
Corresponding author should be written (including institutional address and correcte-mail address).
Second page
Abstract should be written within 300 words.
Add keywords (three to six words) at the bottom of Abstract in an alphabeticalorder.
Third and the following pages
The manuscript should be written in the order of Introduction, Materials andMethods, Results, Discussion, Acknowledgment, Conflicts of Interest, References,Figure Legends, Figures and Tables.
References
References at the end of a sentence in the text should be written as:--- (Simkiss and Taylor, 1971).
If more than two references are cited at the same site in the text:--- (Yoshimura, 1995; Yoshimura and Tajima, 2000; Yoshimura et al., 2004).
If reference is cited at the beginning of sentence:
Yoshimura et al. (2004) reported ---.
The list of references must be in an alphabetical order of the first author's last namein the Reference list with the following styles.
Journal articles:
Hernandez AG and Bahr JM. Culture of chicken granulose cells from small
yellow follicle: A suitable culture system. Journal of Poultry Science 39:14-21.
2002.
Book:
Nalbandov AV. Advances in Neuroendocrinology. 2nd ed. University of Illinois
Press. Urbana. 1963.
Chapter in a book:
Simkiss K and Taylor TG. Shell formation. In: Physiology and Biochemistry of
the Domestic Fowl (Bell DJ and Freeman BM eds.). Vol.3. pp.1331-1343.
Academic Press. London. 1971.
Patent:Suzuki M. Method of in vitro fertilization. Japan Patent No.674562, 2008.
WEB page:
Tanaka M. J-Poultry Web. http://www.j-poult.com/topic20.htm.
Accessed on September 10, 2009.
All the cited papers in the text must be listed in the Reference list.
All the papers in Reference list must be cited in the text

Give marks

Papers not yet accepted for publication should not be listed in References (cite intext as “unpublished data”).
Tables and Figures
Table style and format should be per instructions for authors and recent issues.
Abbreviations used in a table must be defined in that table.
Figures to be printed in black and white should prepared in black and white.
Abbreviations used in the figure must be defined in the caption of that figure.
Supplemental Information
Please provide a one-sentence title and a short legend for each video/audio file. The
legend should be within 100 words and without citation of any reference.
Supplemental data except video and audio files are not accepted on the firstsubmission.
OthersLine numbers should be given on each page.

The Journal of Poultry Science：Check List and Sample Manuscript
1Page 1

2
3

Effects of Repeated Artificial Insemination on the Structure and Function of Oviducal Sperm Storage

4Tubules in Chickens, Gallus gallus

5Shubash C. Das1, Naohiro Nagasaka2 and Yukinori Yoshimura1

67891Graduate School of Biosphere Science, Higashi-Hiroshima 739-8528, Japan
2Animal Research Center, Kochi, Takaoka-Gun 739-1233, Japan

1011Running title: AI and Sperm Storage Tubule1213---------------------------------

14

Correspondence: Dr. Yukinori Yoshimura, Graduate School of Biosphere Science, Higashi-Hiroshima

15739-8528, Japan. (E-mail: yyosimu@hiroshima-u.ac.jp)
Title:Nouns should be written as a capital letters and no period should be put at the end. The scientific names ofanimals and plants, and Latin adverbs should be written in italics or underlined.
Authors:
The first and last names of each author should be written in full spelling. Middle name should be written as theinitials followed by putting periods.
Running Title:
It should be written within 6 words.
Corresponding author:
Name, institutional address, correct e-mail address should be given.

16171819

3

The Journal of Poultry Science：Check List and Sample Manuscript
1Page 2

2

The goal of this study was to determine the mechanism by which the fertility was declined ----

3Keywords: artificial insemination, chicken, fertility decline, oviduct, sperm storage tubules
Abstract should be written within 300 words or less on page 2.
Keywords should be given with three to six words at the bottom of abstract, and arranged in alphabeticalorder.

45Page 3 and following pages
Introduction

67In hens, spermatozoa are stored for prolonged periods after single artificial insemination (AI)8or mating within the sperm storage tubules (SST) which are located in the utero-vaginal junction (UVJ)

9and infundibulum, whereas the UVJ is the primary site for the sperm storage (Fujii and Tamura, 1963;

10Bobr et al., 1964; Lake, 1975). Yoshimura et al. (2005) reported ---------Reference citation in the text:
References in text should be written as last name of the first author, published year in parenthesis.
If the number of authors of one reference is two, last name of both authors should be indicated.
If the number of authors of one reference is more than three, only the last name of first author should beindicated and add “et al.”Plural references in a place should be divided by semicolons and written in order of published year (old tonew).

11Materials and Methods

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Experimental birds
Rhode Island Red hens regularly laying 5 or more eggs in a sequence ----

16Results

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Lymphocytes were distributed in the stroma and the SST epithelium (Fig. 1). Figure 2
shows the results of -----.
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The Journal of Poultry Science：Check List and Sample Manuscript
Discussion

1
2
3
4
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We are reporting the structures and sperm storage ability of SST of Rhode Island Red hens
whose fertility was declined by repeated AI with -------------------Do not make Conclusion section, whereas the study conclusion should be stated at
the end of Discussion.

6Acknowledgements

789This work was supported by Grant-in-Aid for Scientific Research from ----If the work is supported by any grants, Acknowledgment should touch that research grants.

101112131415
16
17Conflicts of Interest
The authors declare no conflict of interest.

Authors must identify and disclose any financial and personal relationships with other people or organizations
that could inappropriately influence their work. If there is no conflict of interest, please state "The authors declareno conflict of interest."
181920212223References

242526(In case of journal article)

27

Nakamura Y, Usui F, Atsumi Y, Otomo A, Teshima A, Ono T, Takeda K, Nirasawa K, Kagami H and28

Tagami T. Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial

29

germ cells in early chicken embryos. Journal of Poultry Science, 46: 127-135. 2009.

30(In case of a book)5

The Journal of Poultry Science：Check List and Sample Manuscript
1Nalbandov AV. Advances in Neuroendocrinology. 2nd ed. University of Illinois Press. Urbana. 1963.

2(In case of chapter in a book)3

Simkiss K and Taylor TG. Shell formation. In: Physiology and Biochemistry of the Domestic Fowl

4(Bell DJ and Freeman BM eds.). Vol.3. pp.1331-1343. Academic Press. London. 1971.

5(In case of WEB page)

6

Tanaka M. J-Poultry Web. http://www.j-poult.com/topic20.htm. Accessed on September 10, 2009.

78(In case of patent)
Suzuki M. Method of in vitro fertilization. Japan Patent No.674562, 2008.
References from journals should be written as:Name of authors (add “and” just before the last author), title, journal name with full spelling, volume,
first and last page, and publication year should be given.
Period should not be put at the initial of author’s name.
The issue number in a volume should be omitted.
Reference from books should be written as:
Name of authors, book name, edition, pp., publisher, published place and published year should begiven.
In case of a chapter in a book, “In: title of the chapter and name of editor(s)” should be given.
References should be listed in alphabetical order of the first author's last name.
Plural references by a same author should be listed by the published year (old to new).
Plural references by a same author published in same years should be listed in alphabetical order of thelast name of second and subsequent authors.
Publications by the same author(s) or by the same first author with the abbreviated coauthors in the text as "et al.'' in thesame year should be listed as 2010a, 2010b. Individual entries should be formatted as follows based on the type ofmaterials cited.

9101112136

The Journal of Poultry Science：Check List and Sample Manuscript
1Legends to figures

2Fig. 1. Structural analysis of sperm storage tubules and lymphocytes population in virgin and3

repeatedly inseminated hens. (a) outer diameter of ---- within each region of UVJ (P < 0.05).

4

Captions of the figures should be placed on one page separated from the figures.
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The Journal of Poultry Science：Check List and Sample Manuscript
1234
5

Examples for Tables and Figures (Prepare on separate pages from the text)
Table 1. Distribution of the reaction products of peroxidase activity in the surface epithelium of oviducts
in immature, laying and non-laying hens
Area of the reaction products distribution (µm2/10,000 µm2)

6Immature－0－0－vagina0a7Laying

00F003,689 ± 335b

Non-laying

00000620 ± 345a

8910Birds

infundibulum

magnumisthmusuterusUVJ

Values in the vagina are mean ± SE (n = 4 each), and those with different superscripts (a, b and c) aresignificantly different (P<0.01). (F) = The positive area was a few. (-) = not examined because theoviduct was not developed in immature hens. UVJ = utero-vaginal junction.

11121314151617181920212223242526278

The Journal of Poultry Science：Check List and Sample Manuscript
134
56
78
910

GAL-12/β-actin mRNA

22.0
1.6

b1.2
0.8

a0.4

aa0.0
0

0.5

12

LPS (mg/kg BW)

11Fig.1 Das et.al1213141516
17Figures (graphs, photographs and illustrations) should be prepared in final printed size in more than 300 dpi. Figures
should not exceed 80 mm (single-column) or 165 mm (double-column) horizontally and 190 mm vertically. Letteringmust be sufficiently large and clear (Century font in 8-point or more). The size of lines in illustrations should be 0.2 mm ormore. Authors will be charged an extra printing fee for the inclusion of color figures, but the expense will be reduced when
the authors submit both color figures for on-line version and black and white figures for printed version.

181920212223242526
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The Journal of Poultry Science：Check List and Sample Manuscript12Supplemental Information

3・Please provide a one-sentence title and a short legend for each video/audio file.
・The legend should be within 100 words and without citation of any reference.
When an editor suggests to add any supplemental figure(s) or table(s), please provide them as
per the instructions mentioned above.
4510

The Journal of Poultry Science：Check List and Sample Manuscript
12（In the case of submission within Japan）

3
ニワトリの卵管精子貯蔵細管の構造と機能に及ぼす高頻度人工授精の影響

45

シュバッシュ C. ダス 1・長坂直比路 2 ・吉村幸則 16781 広島大学大学院生物圏科学研究科

9２東広島市鏡山 739-8528，高知県畜産試験場 高知県高岡郡佐川町中組 789-1233

1011

ニワトリの卵管内で，精子は-------------------。

121314

キーワード：人工受精，受精率低下，ニワトリ，卵管，精子貯蔵管

1511