令和元年度日本植物病理学会九州部会
平成３０年度地域貢献賞受賞者
講演要旨「沖縄県に発生する植物病の診断と防除に関する研究」
沖縄県農業研究センター 河野伸二 氏

座長：岩井 久（鹿児島大学）

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   kawanosh@pref.okinawa.lg.jp.
24℃ 74%

2368mm

AI
ELISA

RIPA

PCR
Agdia

AgriStrip BRA

30
ELISA
ELISA
ELISA
‘

’-6165--59 1984
62 1987
60 1987

8 1996-1-

ELISA

ELISA
ZYMV

Potyvirus

WSMoV Orthotospovirus
PRSV-W Potyvirus

MNSV Gammacarmovirus
1994

1995
CMV

ZYMV WSMoVPRSV-W

CMV Cucumovirus

WSMoV
2011

MYSV

Orthotospovirus

2011

MeMoV Potyviurs
TrMV Potyvirus

1993
1988

PLDMV-C Potyvirus

1997

PLDMV

TrMV

PLDMV-C

TrMV

PLDMV-C

ZYMV

2S142a6

PRSV-W

No.53
76.9%

4 -65

0-

1990

CyMV Potexvirus
23℃
CyMV

CyMV
CyMV

ELISA
RIPA

ELISA

1995

CyMV
-2-

CyMV
DTIBA
‘DIBA’ ‘TIBA’

‘IBA’

DTIBA

DTIBA
ELISA

Triton X-100
CyMV
80%

CyMV

CyMV
23℃
1996
CyMV

1992

RIPA

RIPA

1994
5

CyMV

RIPA
RIPA
RIPA
5A
A

Staphylococcus aureus
IgG

IgG

ZYMV PRSV-W
RIPA

1994CyMV--3-3-

DTIBA

RIPA

1988

Miyakawa & Tsuno 19891994
19971997

DNADNA

1997 Huang et al., 1999
Jagoueix et al. 1996

-PCR1999-Huanglongbing HLB
PCR
2HLB
HLB

2007

90%

HLB

HLB
al., 1999

Huang etPCR Jagoueix et al., 1996 Nakajima et al., 1998

Okuda et al., 2005

PCR
DNA

2009

ICAN

Urasaki et al., 2008

LAMP

PCR

PCR
DNA-4-Urasaki et al., 2007
Nishiwaki etal., 2007 Notomi et al., 2000
ICAN™

Cycleave
HLB
LAMP
ICAN
2009

HLB

PCR- 3

HLB
HLB
HLB

HLB
HLB

21 2009

HLB

HLB
HLBHLBJA

100- --2(1995)
61: 272
Huang, T-H., M-Z. Wu and H.-J. Su (1999) Detection of fastidious bacteria causing citrus greening disease
by nonradioactive DNA probes. Ann. Phytopathol. Soc. Jpn. 65: 140-146.
-5-(2012)5:

139-142
Jagoueix, S., J. M. Bove and M. Garnier (1996) PCR detection of the two Candidatus Liberobacter species
associated with greening disease of citrus. Mol. Cell. Probes 10: 43-50.
(1994)A(SpA-RIPA)
60: 739(1994)(CyMV)

60: 392(1997)
63: 256
Miyakawa, T. and K. Tsuno (1989) Occurrence of citrus greening in the southern islands of Japan. Ann.
Phytopath. Soc. Japan 55: 667-670.
Mukai, H., T. Uemori, O. Takeda, E. Kobayashi, J. Yamamoto, K. Nishiwaki, T. Enoki, H. Sagawa, K.
Asada and I. Kato (2007) Highly efficient isothermal DNA amplification system using three elementsof 5' -DNA-RNA-3' chimeric primers, RNaseH and strand-displacing DNA polymerase. J. Biochem.
142: 273-281.
Nakashima, K., Y. Ohtsu and P. M. P. Maitree (1998) Detection of citrus greening organism in citrus plants
and psylla Diaphorina citri in Thailand. Ann. Phytopathol. Soc. Jpn. 64: 153-159.
Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino and T. Hase (2000)
Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28: e63.
Okuda, M., M. Matsumoto, Y. Tanaka, S. Subandiyah and T. Iwanami (2005) Characterization of thetufB-secE-nusGrplKAJL-rpoB gene cluster of the citrus greening organism and detection byloop-mediated isothermal ampliﬁcation. Plant Dis. 89: 705-711.
(1997)
51: 565-570(1988)
1 Trichosanthes mottle virus

Watermelon mosaic virus 1

35: 1-15
1956

81979

31984

31984

42017

323-6-Potyvirus

2017
1984

31(1981)

Ragged Stunt

47: 697-699.
Kawano, S., E. Shikata, T. Senboku and I. Uyeda (1984) Particle structure and double-stranded RNA of rice
ragged stunt virus. J. Fac. Agric., Hokkaido Univ. 61: 408-418.
Shikata, E., S. Kawano (1984) Small virus-like particles isolated from the rice tarsonemid mites,Steneo-tarsonemus spinki Smiley (Acaria. Tarsonemide)

50: 368-374(1985)

51: 152-158.
(1994)

Cicadulina bipunctella (Matsumura) (Hemiptera:Cicadellidae )

15:51-57.
(1993).

40: 217-219.
(1993).

2Potyvirus.

40: 9-19.

(1994).

29: 9-15.

Maoka, T., S. Kawano and T. Usugi (1995) Occurrence of the P stain of papaya ringspot virus in Japan. Ann.
Phytopathol. Soc. Jpn 61: 34-37.
Yonaha, T., T. Toyosato and S. Kawano (1995) Pepper vein yellows virus, a novel luteovirus from bell pepper
plants in Japan. Ann. Phytopathol. Soc. Jpn 61: 178-184.
Furuya N., S. Kawano and A. T. Keiko (2005) Characterization and genetic status of Banana bunchy top virus
isolated from Okinawa, Japan. J. Gen. Plant Pathol. 71: 68-73.
(2006) Colletotrichum
gloeosporioides().

72: 25-27(2007).

73: 3-8.

Naito T., M. Tanaka, S. Taba, T. Toyosato, A. Oshiro, K. Takaesu, K. Hokama, T. Usugi and S. Kawano (2007)
Occurrence of chrysanthemum virescence caused by “Candidatus Phytoplasma aurantifolia” in Okinawa. J.
Gen. Plant Pathol. 73: 139-141,(2007)
PCR.

73: 25-28.

Urasaki, N., T. Uehara and S. Kawano (2007) Highly reliable polymerase chain reaction assay for citrus
huanglongbing (citrus greening disease) using the sucrose synthase gene as an internal control. J. Gen.
Plant Pathol. 73: 25-28.

Urasaki N., S. Kawano, H. Mukai, T. Uemori, O. Takeda and T. Sano (2008) Rapid and sensitive detection-7-

of “Candidatus Liberibacter asiaticus” by cycleave isothermal and chimeric primer-initiatedamplification of nucleic acids. J. Gen. Plant Pathol. 74: 151-155.
(2008) LAMPDNA.

74: 316-320.

(2009)

Candidatus

Liberibacter asiaticus
55: 62-67.
(2009)
Trichoderma.

3: 17-20.
(2009).

54: 249-251.
(2009)
PCR.

55: 68-75 .

Tomimura K., N. Furuya, S. Miyata, A. Hamashima, H. Torigoe, Y. Murayama, S. Kawano, M.itsuru Okuda, S.
Subandiyah, H.-J. Su and T. Iwanami (2010) Distribution of two distinct genotypes of citrus greeningorganism in the Ryukyu Islands of Japan. Jpn Agric. Res. Quart. 44: 151-158.
Andou T., A. Yamaguchi, S. Kawano, K. Kawabe, S. Ueda and M. Onuki (2010) Ageratum yellow vein virus
isolated from tomato plants with leaf curl on Ishigaki Island, Okinawa, J. Gen. Plant Pathol. 76: 287-291.
(2010).

4: 42-46.

Murakami R., N. Nakashima, N. Hinomoto, S. Kawano and T. Toyosato (2011) The genome sequence of peppervein yellows virus (family Luteoviridae, genus Polerovirus). Arch. Virol. 156: 921-923.
Matsumoto K., R. Yasaka, T. Setoyama, S. Kawano and K. Ohshima (2016) Chilli pepper rugose mosaic diseasecaused by Pepper veinal mottle virus occurs on Ishigaki Island, Japan. J. Gen. Plant Pathol. 82: 57-60.
Murakami R. and S. Kawano (2017) A natural host and diversity of Pepper vein yellows virus Japan. Jpn Agric.
Res. Quart. 51: 59-68.
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2015-8-2012