List of Dissertation Abstract （Environment and Natural Sciences
Name

Supervisor

Title
Synthesis and characterization of

Akiho GOTO

Takashi
AMEMIYA

silver nanoparticles with the useof natural fat-Green chemistry by using
natural oils and fats-

Life Sciences Course）

Abstract
Silver nanoparticles with the use of natural oils and fats were produced by non-aqueous heatdecomposition. By this experiment, it is thought that the silver particles were protected by a coverof fatty acid base. In the past, the silver nanoparticles were synthesized successfully with the use of
single fatty acids. In the present study, it was verified that the silver nanoparticles were composedwith natural fat foe example olive oil, palm oil canola oil. Specifically, it was observed high coatability and viscous property in silver nanoparticle with olive oil.
Gold nanoparticles (AuNPs) are used in various fields such as catalysts, medicals, and biosensors.
Synthesis of AuNPs by reduction in liquid creates by-products including oxidized forms of

Keita

Takashi

AKAZAWAAMEMIYAGold nanoparticle formation by

reductants. These oxidants have to be reduced in order to reuse them as reductants. Hydroquinone

photochemical reaction of(HQ) is suitable as a reusable reductant because benzoquinone (BQ: the oxidized form of HQ) canrecycling reductants

be reduced to HQ simply by photochemical reductions. We thus focused on HQ/BQ redox cycles. In
our system, the more AuNPs were introduced before the reaction, the smaller AuNPs were obtained.
In this study, we propose a new method of controlling the size of AuNPs.
Acidic extracellular pH (pHe) is a feature of tumor tissue. Extracellular acidosis improves cancer
growth and proliferation. Glycolytic oscillations reflect the feature of cancer metabolism. Therefore,

TakumaTakashiASANO

AMEMIYA

Extra cellular pH dependency ofrelationships between cancer metabolism and pHe were investigated by glycolytic oscillations. We

glycolytic oscillations in HeLa

found the ratio of oscillatory cells was changed by pHe.

cervical cancer cells.

7, and highest at pHe 7.2. However, the oscillatory ratio had high deviations. There’s no correlation

The oscillatory ratio was high around pHe

between the oscillatory ratio and the periods. Thus, we conclude that pHe does not influence onreaction rates but affects glucose uptakes.
dCas9/sgRNA system is known as a transcription regulation technique by DNA targeting of
Evaluation of transcription factor
Shuto ABE

CRISPR/Cas9. In this study, we applied dCas9/sgRNA system to transcription regulation in plant

Kazuyukiand systematic regulation of gene cells and discovered a new transcription activator which function on this system. In addition, we

HIRATSUKA

expression by dCas9/sgRNA

attempted transactivation of any genes and developed new techniques for expanding applicability ofsystem in plant cells

this system. Knowledge obtained by this study will contribute to planned modification techniques
for higher plants.

Plants are exposed to various kinds of pathogens at all times, but they have unique disease response
Characterization of Novel Plant
Momoko ABE

Kazuyuki

Activators Identified by High-HIRATSUKA

throughput Screening Using
Luminescence Reporter Genes

mechanism and Systemic Acquired Resistance (SAR) is one of the famous mechanisms. The
chemicals that enhance the SAR is called as Plant activator and they are widely noticed by many
researchers recent years, however, it takes so much time and they are rarely detected from amongenormous amount of materials. We have developed the high-throughput screening system (HTS)
using luminescence reporter gene, then, I found some candidates for novel plant activator and theircharacterization through some tests.
We developed the gene-expression monitoring system with luciferase reporter fused promoter resion

KazukiKazuyuki

Study on the screening system toof a target gene. This system is applied to high-throughput screening for new bioactive compounds.

ISHIDAHIRATSUKA

plant Biostimulants

However, it takes time and effort to produce transformed plants, so further higher throughput was
required. Therefore, in this research we tried to develop a new system using wild-type.
Diketo-prrolo-pyrrole (DPP) derivatives have been studied as a candidate for opto-electornicmaterials in addition to their commercial importance as an organic pigment. Some DPP derivatives
are also reported to have crystal polymorphs with different solid-state properties. Hydrogen bonds

Two crystal polymorphs of aand conformational flexibility are known as a parameter contributes to polymorphic occurrence ofShigetoShinyachlorinated diketo-pyrrolo-molecular crystals including organic dyes. Recently it has been found that modification withOSUMI

MATSUMOTO

pyrrole derivative with hexyl

chlorine and bromine is also expected to promote polymorphic expression . We made an attempt togroup on amino position

investigate polymorph occurrence on a chlorinated DPP derivative with a hexyl substituent on one
amino group. We obtained two types of crystals in different colors and confirmed that these are
crystal polymorphs by SCXRD. Their crystal structures and optical properties are reported in thispaper.
An intermolecular oxa-[4+2] cycloaddition reaction promoted by a thioxanthyliumorganophotoredox catalyst under irradiation with green light has been developed. The reaction ofOrganophotoredox promoted

ortho-quinone methides with styrenes smoothly affords the desired cycloadducts. In particular,

DaichiKiyoshi[4+2] cycloaddition of o-quinone

styrenes bearing electron-donating groups are efficiently transformed in this reaction. This method

OMATA

HONDA

methidesreprents a sustainable way to carry out oxa-[4+2] cycloaddition reactions using only a catalytic

under green light irradiation

amount of a photo-catalyst and visible light.

YuiTakashi

KAMEYAMA

AMEMIYA

Influence of alcohols on
glycolytic oscillations and theirsynchronization

Glycolytic oscillations are known to synchronize by acetaldehyde and so on. We found alcohol
promotes the synchronization of glycolytic oscillations. Therefore we quantitatively evaluated theinfluence of alcohol. Our results demonstrate that synchrony is changed by alcohol chain length andthe concentration.
We made an attempt to obtain polymorphs of N-monopropylated diketopyrrolopyrrole dye andJunna

ShinyaKAWAGUCHI MATSUMOTO

Occurrence of polymorphs andobtained eight different kinds of crystals using various solvent conditions in crystallization. The

pseudopolymorphs of a diketo-obtained crystals showed several differences in colour and shape. After analysing their crystal

pyrrolo-pyrrole derivative withstructures, we found three differently coloured polymorphs and five pseudopolymorphs with same

propyl group on amino position.

red colour. In this paper, physical properties of eight crystals were investigated based on the crystal
structures.

Localization of the exogenous

ATP is synthesized in the electron transport system in mitochondria in cells, and the subunit protein

Nobuyuki

Shinichisubunits of Complex IV ofof complex IV has an important role to control its activity. In order to analyze its function, this study

KAWACHI

OGATA

mitochondrial electron transport

explores the subcellular localization of the expressed exogenous subunit protein and the conditions

chain

necessary to show localization similar to the endogenous protein.
Bisphenol-S (BPS) derivatives have been used as color developer in high performance thermal

The investigation of the black
TakuyaShinyasolids prepared from fluoran dye,

KIKUCHIMATSUMOTO

bisphenol-S type developers andsensitizers

paper. The derivative 1 which has two allyl groups is known to have high color sensitivity andexcellent image stability as compared with those of BPS. We have studied crystal structure of 1 anda common fluoran dye ODB2 in order to interpret this developer characteristics in terms ofmolecular structure. We attempted to crystallized BPS with ODB2 and investigated amorphous blacksolids of these developers and ODB2. Furthermore, we focused on sensitizers in order to interpretthe influence of sensitizers on physicochemical properties of thermal paper.
Organic dyes are used as functional materials such as recording materials and electronic materials.
When used in these applications, organic dyes are often used in a thin film state. Therefore,

Yuko KISHI

Phase transition behavior of thin

examining the physical properties and structure of the dye in a thin film state is important for a

Shinyafilms of several 2,5-diamino-3,6-functional material. In this study, we have prepared vacuum deposited films of several

MATSUMOTO

dicyanopyrazine dyes having

monosubstituted benzyl derivatives of this dye. The prepared films of the tested derivatives weremonosubstituted benzyl groups

found to be amorphous. These amorphous films can be transformed to crystalline films by heat orsolvent vapor treatment. In this paper, we also report the structural features and spectroscopic
properties of these thin films.

Photoredox catalysts can be used to carry out a wide variety of organic transformations. Althoughthese attractive catalysts have efficiently used under visible light irradiation, they need to use highSynthesis of newenergy light source such as blue light (400-500 nm). Now, we report green light-driven radical

Mami

Kiyoshiorganophotoredox catlalysts andcation Diels-Alder reaction catalyzed by thioxanthlium-based organophotoredox catalysts. TheseKISHIMOTO

HONDA

their application to radical cation

catalysts present high excited-state reduction potentials, and they are able to efficiently activate

Diels-Alder reaction

dienophiles under green light irradiation afforded the targeted radicalcation Diels-Alder
cycloadducts in good yields. The present photoredox catalytic system provides a sustainable
synthetic process.
Our genes are constantly damaged by radiations and chemical substances. Since these damages

Norihito

Shinichi

KOWADA

OGATA

Attempt to isolate mutant related

affect life activities, we have various mechanisms against them. Now, we focused on a DNA double

to DNA repair process by

strand break (DSB) and homologous recombination (HR) as a repair mechanism for DSB. Althoughtransgenic Arabidopsis thaliana

HR is an important repair mechanism for DSB, the process from damages to HR is not completely
revealed. We try to reveal it by using transgenic Arabidopsis thaliana.
Plant defense activator attracting attention reacent years is a low toxicity and environmentally

Identification of a compound that friendly pesticide that is hard to appear drug resistant bacteria.I searched for plant defense activator
HideakiKazuyuki

induces Arabidopsis VSP1 genethat acts on JA signaling pathway. Especially, I used pliming efeect that plants stimulated compoundSAITO

HIRATSUKA

promoter using bioluminescenceor pathogen show stronger resistance to secondary stimulation. As a result, a candidate compoundmonitoring system

S1 acting on JA signaling pathway was discovered. Further, a detailed experiment was conducted on
the candidate compound S1.
Recently, the number of prostate cancer patients is sharply rising in Japan. There are several
treatments of prostate cancer, but each treatments has some kind of demerit. Development of

Basic research on arrest of
Rina

Takashi

SAKAMAKI

AMEMIYA

proliferation in prostate cancer
cells by suppression of gene
expression and inhibition of
catabolic pathway

effective treatments is needed currently. In this study, the effects of suppression of gene expression
and inhibition of catabolic pathway on prostate cancer cells (DU145) were investigated. We used the
KIF22 siRNA to suppress gene expression and exposed DU145 cells to oxamic acid to inhibit
catabolic pathway. Our results suggest that the combination of knocking down KIF22 gene andexposure of oxamic acid decrease the number of DU145 cells.

Chiral natural products containing 3-chromanol or coumaran core structures are well known

Masato SEKI

Synthesis of chiral hydroxamic

compounds, some of which exhibit various attractive bioactivities. Although several methods ofacids containing 1,1'-

preparing them have been reported, the progress of the study on asymmetric epoxidation of o-Kiyoshispirobiindane core and their

allylphenol, followed by ring opening-closing reaction for preparing 3-chromanols and coumarans

HONDA

application to catalytic

has been rather slow. we synthesized spiro-CHA and spiro-HA containing 1,1’-spirobiindane core.

asymmetric epoxidation of o-Furthermore, We performed that the asymmetric epoxidation of o-allylphenol catalyzed by HA-

allylophenols as ligands

metal complexes, followed by intramolecular cyclization under acid conditions, gave 3-chromanols
and their enantiomeric excesses were determined by chiral HPLC.
Two π-extended macrocyclic oligothiophene 6-mer, 6T4A-Ph and 6T4A-H, linked with four triple
and two double bonds were synthesized by using a modified McMurry reaction. In addition, I

Masataka

Hiroyuki

TAKASHIKAOTANI

Synthesis, structure, properties
and multifunction of p-expanded
cyclic oligothiophene 6-mers

investigated structure, physical properties, and function of those oligothiophene 6-mers. Phenyl
substituted 6-mer 6T4A-Ph has easily form polymorphs involving solvents. The yellow fibers
prepared from CS2/Acetone exhibited vapochromism and showed shape changes. Those functions
can be repeated several times by vapor-induced quasi-reversible. Furthermore, unsubstituted 6-mer
6T4A-H forms a p-dimer having a planar structure and shows organic field-effect transistor
behavior.
Compound X identified using the HTS system shows affinity for bacteria-derived SA receptors andStudies on Screening and

can induce resistance, as a plant activator such as acibenzolar-S-methyl and salicylic acid. It was

KeikoKazuyukiCharacterization of a Novel

found to have an interesting feature of inducing the expression of the PR-1a gene promoter. The

TANAKAHIRATSUKA

Rapid-response-type Plant

details of the gene expression induction pattern of Arabidopsis thaliana by compound X treatment

Activator

and the induction of the expression of defense response-related genes in rice leaf sheath were
investigated.
Tropolone forms chelate complexes with various divalent transition metal ions via the carbonyl oxygenand vicinal hydroxy group. Structural analysis and electronic structural analysis of D-A biphenyl type

Nao TAMAKI

Hiroyuki
OTANI

Synthesis, structure andtropolone dye, D-π-A tolan type tropolone dye, and D-π-A terphenyl type tropolone dye that hasproperties of the D-π-A tropolone thiophenyl as a spacer were carried out by DFT calculations. Comparing these results, the effect of the πdyes for dye sensitized solar cells spacer on the properties of the tropolone dye was demonstrated. In addition, two new type D-π-A πexpanded tropolone dyes having ethynylthiophenyl as a spacer were synthesized. Furthermore, I
investigated absorption properties, in solution and in the solid state, and redox behavior.

Anthracene cyclic dimer linked with two biphenylylens 1 was synthesized by using the electron-

Yuki

Hiroyuki

NAKAMURAOTANISynthesis, Structure andtransfer oxidation of Lipshutz cuprate, derived from 1, 8-bis(4-bromophenyl)-10-mesitylanthracene.

Properties of Anthracene Cyclic

In addition, anthracene cyclic dimer linked with two troponoid complexes 2 was prepared by mixing

Dimer Linked with

1,8-bis(2-dodecylaminotropon5-yl)ethynyl]-10-dodecylanthracene, as a precursor, with a divalent

Biphenylylene Unit or Troponoid transition metal ion (Cu2+ or Ni2+). Both anthracene cyclic dimer 1 and 2 can be employed inComplex Unit

constructing a macrocyclic framework having a small inner cavity. I investigated the structure,physical properties, and complex formation behavior of those anthracene cyclic dimers.
It has been reported that 1-cyano-trans-1, 2-bis- (4'-methylbiphenyl) ethylene as a 1-cyanostilbene
derivatives shows an unusual effect of aggregation induced emission (AIE). Herein, I synthesized
four new benzo-annulated 1-cyanostilbene derivatives, 1-naphtyl derivative 1, 2-naphtyl derivative

Synthesis, Structure and2, 9-anthryl derivative 3, and 9-phenanthryl derivative 4. In addition, I investigated structure, optical

Ryota

HiroyukiAggregation Induced Emission

properties, and polymorphs of those. Interestingly, molecules 1and 3 were non-emissive when

NAKAMURAOTANIof Benzo-Annulated

dissolved in a good solvent but became luminescent when aggregated in the crystal state.

Cyanostilbene derivatives

Furthermore, molecules 1 and 2 became highly luminescent when aggregated in the lamellar crystal
state. These results indicate that 1, 2, and 3 are molecules showing AIE effect. On the other hand,molecule 4 was higher luminescent when dissolved in a good solvent than when aggregated in the
crystal state. 4 was molecule showing aggregation-caused quenching (ACQ) effect.
Various plant viruses have the function of performing efficient gene expression while avoiding the

ShingoKazuyuki

HASHIMOTO HIRATSUKA

Activity evaluation of silencing

host defense response such as RNA silencing and they can be applied to the high efficiency of

suppressor derived from several

expression and expression of foreign genes in plant cells. Efficient utilization of IRES (Internal

plant viruses

Ribosomal Entry Site) and RNA Silencing Suppressor (RSS) derived from the plant virus-derived
factor, We created platform plants that are the basis of material production.

Studies on runnel drainage
derived sheath bacterium ATCC
Atsuo

ShinichiBAA-2725 strain and

FUJIWARA

OGATA

polysaccharide elimination
enzymes related to sheath formed
by ATCC BAA-2725 strain

Sphaerotillus is a filamentous bacterium found in the aquatic environment. Bacteria of the genus
Sphaerotillus form a microtube called a sheath outside the bacterial cells, and rod-like cells are containedin it. ATCC BAA-2725 strain is Sphaerotillus bacterium isolated from runway drainage system. For thisstrain having a special habitat environment, we investigated carbon source utilization and sheathelongation pattern and compared it with conventional Sphaerotillus bacteria. In addition, detailed analysis
was also performed on the substrate specificity of DssA, a polysaccharide elimination enzyme thatspecifically degrades the sheath formed by Sphaerotillus bacteria.

π-Extended macrocyclic oligothiophene 7mer 7T6A-14Bu and giant macrocyclic oligothiophene
Synthesis, Structure, andKeigo

Hiroyuki

Properties of π-extended

MISHIMAOTANIMacrocyclic Oligothiophene 7mer and 14-mer

14mer 14T12A-28Bu, containing 3,4-dibutylthiophene as a constituent, were synthesized using theMcMurry coupling reaction of linear oligothiophene 7mer dialdehyde. The molecular structure of
7T6A-14Bu and 14T12A-28Bu was revealed by X-ray crystal structure analysis. Interestingly,
14T12A-28Bu formed various polymorphs from solution, reflecting a disklike structure with partial
rigidly. Furthermore, 14T12A-28Bu formed CT complex with chloranil in solution and in the solidstate and gave complex with TCNQ in the solid state.