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Overseas Reports【Current Activities of a Former Participant】

Lu-san reported her current activities in China.

1) Course Leader:

Junji KAWASAKI2) Former Participant:

Ms. LU Jing

October 20th, 2015
Training Division

Kitakyushu International Techno-cooperative AssociationWe report on activities being run by former participants playing an active role intheir homeland after completing their JICA/KITA training course

This issue of Overseas Reports covers activities being run in their homeland by participants whoattended the training course, “Industrial Wastewater Treatment Techniques (A)”. Junji KAWASAKI,
the course leader who managed the course, introduced us their activity situation.

1. Introduction of the participant, who has given the report
Nickname

Name

Country

Period of JICA
Training Course

Ms. LU Jing

P. R. China

Jun. 16, 2013 ～Sep. 07, 2013

Photo

Lu - sanCourse leader
J.KAWASAKILu - sanLu-san after a lecture, with participants,
Mr. KAWASAKI and the coordinatorLu-san at a company visited inthe course

12. Message from Junji KAWASAKI, Course Leader
When we talk about environmental problems in China, we have plenty oftopics like air pollution by PM2.5 and cancer villages caused by industrial
wastewater. A former participant, Lu-san from China who work as auniversity lecturer, reported to me about her research of wastewater
treatment technology.
She is trying to apply the study of wastewater treatment using Chloroperoxidase as acatalyst, which is conducted partially in Japan. However, it is rare to apply to dyeing wastewater treatment because it is difficult to handle. She acquired a large amount of knowledge
about theory of dyeing wastewater treatment by site visit in Japan. In this report, she tried
to achieve advanced treatment.
She works at a university in Shaanxi Province which is famous for The Museum of Qin
Terra-cotta Warriors and Horses located in the middle of the Yellow River in the center ofinland areas of China. It is surprising that the advanced research is conducted in such areas,
and this shows the effort of solving the environmental problems of present China. I believethat the environmental pollution problems will be overcome by continuing the effort.
During the training in Japan, she always kept a smile and was kind to others. I
remembered her smile reading this report and felt the training in Japan blossomed into great
results where we do not know well.

3. Letter from Lu-sanI work as a part-time lecturer in Shaanxi University of Science and Technologyin China. I give lectures and do research in chemistry. Recently, I focus ondyeing industrial wastewater treatment and work hard with my colleagues todevelop new bio reactor which is very effective and high reliability at relatively
low cost as dyeing industrial wastewater treatment.

2Title of Report and Contents【 Immobilization of Chloroperoxidase on Materials andIts Application in Dye Decolorization 】

From 2014 to 2015, I have been doing scientific research on enzyme immobilization andits application in dye wastewater treatment.
With the development of printing and dyeing industry, the degradation of dyes in a
waste stream generated by textile photography industries is a main issue in wastewater
treatment. The dye wastewater is characterized with high value of CODcr complication oforganic components, and high colority, which is one of the main challenges for sewage
treatment, there are some defects in this current method for degradation, such asflocculation, adsorption and other physical methods and the Fenton reagent oxidation andother chemical methods, which physical methods cannot be completely degrading pollutants,
and can do nothing for dyes; a variety of chemical methods exist to varying degrees of poor
color removal, the treatment method is complicated and a variety of secondary pollution.
However, currently used in microbial degradation due to slow degradation rate and thedegradation objects is limited, the treatment pond is a large space and other factors.
Chloroperoxidase (CPO) is a heme sugar protease. It has a wide range of substrate
adaptability, and now becomes the most widely used enzyme in the family of peroxidase
which can be used to catalyze multiple types of reactions, such as halogenation, peroxidation,epoxidation, sulfoxidation, and hydroxylation, etc. CPO has good application prospects
because of its stability, which can keep activity for 2 ~ 3 years at 4 ℃. Our group hasreported the outstanding performance of CPO in degradation of water-soluble dye.
At the optimum reaction condition, the decolorization efficiency of 0.1 mM croceinorange G. reached 97.31% in 5 min at the initial CPO concentration of 0.4μM; 90% ofdecolorization in 5 min for Alizarin Red, Crystal Violet, Orange G、Orange Ⅳ and Alizarin
Yellow. In addition, 88% in 5 min for Orange Ⅱ, Metanil yellow, Congo Red. Therefore, CPO
is expected to be applied in the wastewater treatment industry.

3However, there are many difficulties in practice, such as temperature, pH ofwastewater and use of organic solvents. The enzyme is easy to lose its activity in extreme
conditions. At the same time, the free CPO cannot be isolated from the reaction system forreuse.
Immobilizing the enzyme molecules on a solid phase carrier is a common method tosolve this problem. The carrier and immobilization methods have a vital influence on thecatalytic activity of immobilized enzyme. Several materials such as mesoporous SiO2,
CaCO3/lentinan, graphene oxide have been selected for CPO immobilization carrier in ourstudy. The operational stabilities, including thermal stability, acid and alkali survivability,storage stability to H2O2 concentration, have been enhanced significantly.
At present, immobilized technology are often accompanied by aggregation of theenzyme and reducing the enzyme activity. To overcome this shortcoming, we attempt toprepare new mesoporous material as carrier which just match the size of enzyme molecule,avoiding aggregation of enzyme. We are looking forward a satisfactory result in the future.

4

Photo Album of the JICA/KITA Training Course

Closing ceremony of “Industrial Wastewater Treatment Techniques (A)”course, September 06th, 2013

Lu-san with Kawasaki course leader

Field study at the course

The participants on a field trip

Visit to Hot spring5