List of Dissertation Abstract (Department of Natural Environment)
Name
Satsuki CHIBASupervisor
Kazuyuki

Title

Abstract

Characterization of artificial

Artificial transcription factors can be applied to plants toArabidopsis NPR1 gene promoter.

Therefore, artificial transcription factors are useful for

HIRATSUKA transcription factors targeted to thecontrol the expression of arbitrary endogenous genes.

constructing plant-based material production systems

and creating high-value-added plants.In order for an

artificial transcription factor to function effectively, it isnecessary to recruit the transcription factor to an optimalposition, but there is still little knowledge about thetarget sequence. In this study, we aimed to establish atranscriptional regulatory system applicable to various
gene promoters, examined target sequences for theArabidopsis thaliana NPR1 gene, and worked on efficienttranscriptional activation.

Yoko ARIMURA

Tomohiko

KIKUCHI

Effect of light environment on

In this study, we focused on the variation of the optical

phytoplankton mainly diatoms

diatoms, and aimed to understand the effect of changes

vertical distribution of

depth in the ocean and the vertical distribution ofin the light environment in water on the vertical
distribution of diatoms.

This time, 79 species of 32 genera were observed on theManazuru Peninsula st.M in Sagami Bay. Furthermore,
the relationship between the penetration depth andnutrients by genus Diatom was examined. The results

suggest that the appearance time, cell number density,and vertical distribution vary depending on the lightenvironment even in an environment where nutrients aresufficiently present.

Rie UCHIDA

HirokiOIKAWAA study on the respect for property

The “property rights respect clause” stipulates thatCultural Properties

individual acts. The purpose of this study is to examine

rights in the Act on Protection ofproperty rights must be “respected” in the application ofwhere the Diet falls in this debate and how it has handled
the addition of the property rights respect clause to theAct on Protection of Cultural Properties (APCP). The biggest problem in the discussion of theclause is that it does not take into account how it wasadded.It should be necessary to review the way in which

the law is criticized and refuted before deciding whether
the provision that respects property rights should beapproved.

Kouhei KANDA

TatsuoCell selectivity and magnetic

Magnetic hyperthermia treatment (MHT) is an attemptferrite nanoparticles

nanoparticles to yield thermal energy in AC magnetic

NAKAMURA hyperthermia effects of Mn-Zn

to make the cancer treatment by using the magnetic

field. Mn0.8Zn0.2Fe2O4 nanoparticles surrounded byhighly hydrophilic material of polyethylene glycol (PEG)
were prepared by One-pot solvothermal synthesis. For
the application of MHT, we modified glucose intonanoparticles surrounded by PEG and examined thethermal effects of nanoparticles in vitro experiments
using cancer cells.

Hiroshi KINO

Fumito

KOIKE

Vertical distribution of flying

arthropods in metropolitan area

Insect flight is an important behavior in life history andhas a three-dimensional distribution in horizontal andvertical directions. Investigating the spatial distributionof flying insects at high altitudes is difficult, especially inurban areas. In this study, we investigated three-dimensional insect distribution in urban space in thesouthern Tokyo metropolitan area. A total of 4437

individuals were collected on the 377 survey days. In

order level, Dipteral, and Hymenoptera, Hemiptera, infamily level, Chalcidoidea, and Chironomidae, Sciaridae(include Mycetophilidae) were collected. On thefirst floor of the building, Aleyrodidae were dominant,and the others were dominated by Chalcidoidea, andChironomidae.

Tomoya

KUROSE

Fumito

KOIKE

Relationship between walking routewith dog and green space

In this study, we focused on "walking dogs" and examined
the relationship between the walking route and the green
space environment. As a method, a questionnaire wasgiven to people walking dogs in Kanagawa Prefecture,and they filled out their usual walking route. The
preference of the environment was quantified bycomparing the environment of the walking route with theenvironment of the surrounding area. As a result, it was

found that the open green space environment was morethan twice as much favored as the urban area.

Kureha SUZUKI Akira MORI

The potential roles of alien species

The global forest restoration needs to occur in order to(Larix kaempferi)degraded forests. Alien species are regarded as threats toon forest restoration: Japanese larch enhance biodiversity and carbon sequestration innatural-forest restoration focusing on construction andcomposition of native tree species. In this study, I

evaluated the role of Larix kaempferi, which is a domestic
alien species in Hokkaido, on natural-forest restoration

within a restoration area in Shiretoko National Park in
Hokkaido. I found that this alien species had positiveeffects on native tree species. L. kaempferi plantations

promoted height growth of native species and provided

suitable habitats for regeneration of them. These resultssuggest that alien species could contribute to naturalforest restoration.

TomokiTAKATORI

Akira MORI

Responses of taxonomic and

One primary goal in community ecology is to identify theherbaceous plant communities toHere, I examined the response of plant community to thefunctional composition of
rainfall changes

community assembly process under changing climate.
rainfall manipulation at semi-natural grassland in

Hokkaido Japan. I collected seven functional traits andevaluated how taxonomic and functional composition

related to soil environmental variables. As a result, thesoil moisture increased by the irrigation, reducing species
evenness and functional redundancy, and increasing
functional diversity. These results suggest increased
rainfall cause competitive exclusion among similar

species of functional traits, decreasing stability of plantcommunity structure and increasing vulnerability toother environmental extreme changes and perturbations.

Hiyori

Shinichi

TAKABAYASHI OGATA

Reducing false negative results in

For animal welfare, it is necessary to ban animal

The human cell line activation test

process. In vitro skin sensitization test : human Cell Linean in vitro skin sensitization test :experiments related to the cosmetics manufacturing

Activation Test (h-CLAT ) Has been developed, but theissue of false-negative results remains. In this study,

expression of CD86 and CD54 was measured using realtime PCR to simplify the evaluation method and shorten

the evaluation time. We are investigating the detection ofa response that is more sensitive than surface antigens,and aim to improve false-negative results by improving

the evaluation sensitivity.
TaikiTACHIBANA

TakehiroSASAKI

Effects of plant diversity on seed

Human activities among the world decrease biodiversity,

visiting insects : evidence from aHowever, the relationship between plant diversity andproduction mediated by flowerplant removal experimentand as a result, the structure of ecosystem is changed.
multitrophic ecosystem functioning is not revealed.

Based on these matters, I investigated the relationships
between plant diversity, pollination and seedproductivity. According to the research in Inner

Mongolia, the relationship between plants and pollinators
is affected by plant diversity. Moreover, because of thischange, seed productivity has been changed.Mio TOMITA

KazuyukiCharacterization of a novel

Plants are exposed to various kinds of stress every day,signaling pathways.

themselves from these. One of these defense mechanisms

HIRATSUKA compound that act on multiple

and have their own defense mechanism to protect

is Induced Systemic Resistance (ISR). The chemicals thatenhance the ISR is called as Plant activator and they arewidely noticed by many researchers recent years,

however, it takes so much time and they are rarely

detected from among enormous amount of materials. We
have developed the high-throughput screening system(HTS) using luminescence reporter gene, then, I foundsome candidates for novel plant activator and theircharacterization through some tests.

Koretaka

NAKATANI

Ryuichi

MAJIMA

Sedimentary facies and sedimentary I researched lithography and paleocurrent of the Nojima
environment of Lower Pleistocene

Formation in the Kaiyama Park, along the Tokyo Bay inGroup along Tokyo Bay, Miura

Formation exposed in Kaiyama Park is 53 m. Lithofacies

Nojima Formation of the Kazusa
Peninsula.

northern Miura Peninsula. The thickness of Nojima

is composed mainly of muddy sand stone and interbedsandy mud stone, fine ~ course sand stone andconglomarates including shell fragment. Reunion

subchronozone, paleomagnetic boundary which is 2.142.15 Ma is included in Kaiyama Park. Paleocurrent

estimated arrangement sand grain showed north andsouth. I think that I need to consider that from now on.

Hiroki NAMBA

Hiroyuki

MATSUDA

Comparison of macroinvertebrate

In this study, we evaluated the ecological impacts ofreceiving mine drainage andfish fauna in rivers in northern Japan where treated mineand fish assemblages in a river
uncontaminated river

metal pollution by investigating macroinvertebrates andwastewater flows. Concentrations of Cu, Zn, Pb, and Cd

at downstream pollution sites were 0.1 ~ 1.5 times higher
than US EPA water quality standards, with little effect onmacroinvertebrates and fish populations. At two

contaminated sites 0.8 ~ 3.7 times higher than the water
quality standard, statistically significant decreases in themetrics of a few macroinvertebrates communities, such as
the richness of mayfly and the abundance ofHeptageniidae, were detected, but no significant effects
on the abundance and condition factors of 4 dominantfish species, including masu salmon, were detected.

These results suggest that richness and abundance ofmacroinvertebrates in this river are more sensitive tometal contamination than the abundance and condition
factors of fishes.

ShihoNISHIZAKA

Akiko
SAKAI

Spring leaf phenology variation of

Spring leaf phenology is important for carbon gain

individuals relating to verticalinvestigated leafing day of Fagus crenata trees in various

Fagus crenata inter and intra

foliage position in a forest, snowy
regionstrategy in temperate deciduous forests. In this study, Isizes, focusing on relative height of shoots in the tree

crown and relative height of the tree in the forest. For

foliage not affected by snow, smaller trees opened leaves

earlier, and flushing day also changed within a crown. For
foliage not affected by snow, flashing occurred earlier intaller trees and higher shoots within a crown, and shoots
under lower illuminance after F.crenata flushing opened
earlier. It was suggested that passive response againstsnow and strategic response to avoid shading are bothdrivers of spring leaf phenology, in the beech of snowyregion.

Miyu NITTA

FumitoKOIKE

Upper limit of riverside plant
distribution

The river has developed gravel riverbanks composed ofoligotrophic and gravel soils. In this study, we aimed toclarify the upper limit of the distribution of these plant
species. The survey sites are six water systems in

Kanagawa Prefecture, and run up from near the mouth ofeach river,a total of 17 native and 14 non-native species

were surveyed at 77 sites where the gravel basin wasdiscovered. From the analysis results, the upper limit ofthe distribution of the native species is clear, and theupper limit of the alien revegetation species is distributed
in rivers with a large water system, and the upper limit isunclear. It was suggested that the distribution could beexpanded by planting.

Mayuka MIWA

Shinichi
OGATA

A study of AtRad51D gene that will

AtRad51D is related to structural changes inchromosomes of Arabidopsis

response. Arabidopsis thaliana is widely used as a model

associate sith structural changes inthaliana

chromosomes, and may be a key player in plants immune
organism in plant biology. This plant has the AtRad51

protein family. This family proteins are related to searchfor homology and strand pairing stages of DNA

homologous recombination. And chromosome structurechanges at the same time. AtRad51D is a member of theprotein family, and its information is relatively poor. Soour big goal is to reveal function of this protein in detail.

NanamiYOSHIOKA

Ryuichi
MAJIMA

Molluscan fossils and sedimentary

I used Molluscan fossil for estimate the sedimentary

Pleistocene Nojima formation ofRyokuchi Park and the Nojima Park, exposed along theenvironments of the Lowerthe Kazusa Group, exposed along
Tokyo Bay of Miura Peninsula

environments of the Nojima Formation in the Kaiyama

Tokyo Bay in the northern Miura Peninsula. The water
depth at the time of sedimentation was estimated to beabout 50 m in the Kaiyama from the habitat depth of thesame species as the present species. In addition, many ofthe species produced showed cold sea conditions. Nojima
Park estimated that the water depth at the ti

me of sedimentation was 100-200 m. It is probable thatKaiyama was deeper than Kaiyama to Nojima Park
because it was lower than Nojima Park.