Solvents for Liquid
Chromatography

・ for HPLC・・・ p.2・ for LC/MS・・・ p.3

・ for QTofMS・・・ p.5・ for GPC

・・・ p.7

・ for JP General Tests

・・・ p.8

・ GalloTect™

・・・ p.9

LC solvents of FUJIFILM Wako Pure Chemical Corporation
As various chromatograph detectors become more improved, the quality
demanded in the mobile phase (solvent) has become higher and more diversified.
We have a lineup of solvents for high-performance liquid chromatograph (HPLC) thatcan be used in LC analysis in general, solvents for high-purity LC/MS, and solventsfor QTofMS, which is suitable for ultrahigh sensitivity MS analysis. We also offer
optimal solvents for use in GPC, and solvents that can be used for JapanesePharmacopoeia tests.

List of testing requirement for LC solvents

The proof test items vary even for the same solvent, depending on the detector inwhich it is expected to be used or the application.
The solvents under each standard have passed the testing requirement that aresuited to the “expected applications,” in addition to the quality test as chemicalsubstances.
Requirement

GradeHPLC

Absorbance

Gradient test

Fluorescence
test

LC/MS

Absorbance

Gradient test

Fluorescence
test

Particles

Suitability for
LC/MS analysis

QTofMS

Absorbance

Gradient test*

Fluorescence
test

Particles

Suitability for
QTofMS analysis

GPC

Absorbance

Gradient test

Suitability for
GPC analysis

the JP General
Tests

JP method

USP-NF method

EP method*The gradient test for QTofMS was conducted as a QTofMS-compatible test.

Description of testing requirement
AbsorbanceA test to confirm that the peak hight that interferes with the analysis is lower thana certain standard level at a specific wavelength.

Gradient testA test to confirm that the fluctuation of the baseline is lower than at the standard
level when it is used in gradient elution.

Fluorescencetest

A test that compares the quantity of impurities with fluorescence that cause the backgroundnoise or deterioration in detection sensitivity when it is used in fluorescence measurementwith the reference sample by the fluorescence intensity.

Particles1

A test to confirm the number of particles that may affect the column life.

Suitability for
LC/MS analysis

A test to confirm that the background noise is suppressed to a low level when it is used inanalysis using a mass spectrometer.

Suitability for
QTofMS analysis

A test to confirm that that baseline fluctuation is lower than the standard level by connecting
a column to UHPLC and conducting gradient measurement using QTofMS.

Suitability for
GPC analysis

A test to confirm the absorbance after heating with consideration of solvent discoloration
caused by heating during polymer dissolution and analysis.

for High Performance Liquid Chromatography （HPLC）
Absorbance

Gradient test

Fluorescencetest

We offer various solvents of quality appropriate for use in high-performance liquid chromatograph.

Product list (Solvents for HPLC)
Product Number
014-08681
010-08683
019-08631
015-08633
025-06691
023-10801
024-12771
033-08631
039-08633
031-20531
037-20533
033-08511
039-08513
042-16691
046-18671
042-20621
048-20623
046-16971
042-16973
136-06751
132-06753
056-03341
052-03343
057-03371
053-03373
085-06991
087-06995
084-03421
080-03423
085-03691
138-06473
132-06471
162-13461
165-09161
161-09163
200-19391
206-19393
209-06811
205-06813
207-06731
209-06791

Product Name
AcetoneAcetonitrileBenzene
1-Butanol
t-Butyl Methyl Ether
Chloroform
Chloroform, Amylene added
Cyclohexane
1,4-Dioxane
o-Dichlorobenzene
N,N-Dimethylformamide
Distilled Water
Dichloromethane
Ethanol (99.5)
Ethyl Acetate
1,1,1,3,3,3-Hexafluoro-2-propanol
HexaneHeptaneMethanol
1-Propanol
2-Propanol
Tetrahydrofuran, with Stabilizer
Tetrahydrofuran, Stabilizer Free
2,2,4-Trimethylpentane
ToluenePackage Size
1L
3L
1L
3L
1L
1L
1L
1L
3L
1L
3L
1L
3L
1L
1L
1L
3L
1L
3L
1L
3L
1L
3L
1L
3L
100 mL
500 mL
1 L
3L1L
1L
3L
1L
1L
3 L
1L
3L
1L
3L
1L
1L
2

for LC/MS
Absorbance

Gradient test

Fluorescence
test

Particles

Suitability forLC/MS analysis

These are dedicated solvents for LC/MS for which LC/MS compatibility has been confirmed.
They are also recommended for ultrahigh-performance liquid chromatograph (UHPLC), as they
are guaranteed for particles.
A glass bottle with special treatment to suppress the elution of impurities is used as thecontainer*.
*Patent No. WO/2005/047884

Point for high recommendation 1
The cap material for LC/MS solvents was changed from aluminum to a special resin.

While the conventional aluminum caps posed a risk of aluminum dust adhering to the bottle
mouth and contaminating the solvent, the new resin cap has no dust generation orcomponent elution from the material. It maintains the high-quality state of the solvent.

Point for high recommendation 2
The quality was improved further by a review on the manufacturing method.
The baseline is suppressed at an even lower level compared to the conventional products.

Comparison of baselines for acetonitrile (comparison of our products)

Before improvement →After improvement →3Example of product specifications (acetonitrile)
REQUIREMENTSPECIFICATION

Assay（％）
Density（20℃）[g/mL]
Refractive index nD２０
Water [％]
Residue after evaporation [％]Acidity（as CH3COOH） [％]Ammonium（NH4） [ppm]Peroxides（as H2O2）[ppm]Substances reducing permanganate (as O)
Gradient test

min. 99.9
0.780～0.783
1.343～1.346
max. 0.03
max. 0.001
max. 0.001
max. 0.3
max. 5to pass test
to pass test

Particles（0.5μm or more)[ number of particles per mL ]Absorbance

200nm
210nm
220nm
230nm
240nm
Fluorescence test
Suitability for LC/MS analysis

max. 100
max. 0.04以下
max. 0.03以下
max. 0.02以下
max. 0.01以下
max. 0.005以下
to pass test
to pass test

Product list (Solvents for LC/MS)
Product Number
012-19851
018-19853
050-09221
056-09223
062-04721
068-04723
138-14521
134-14523
168-25531
164-25533
214-01301
210-01303

Product Name
Acetonitrile
Ethanol (99.5)
0.1vol% Formic Acid-Acetonitrile

Package Size
1L
3L
1L
3L
1L
3L
1L
3L
1L
3L
1L
3L

Methanol
2-Propanol
Ultrapure Water

Product list ( Mobile phase additives for LC/MS)
Product Number014-20063
018-20061
063-04533
067-04531

Product Name
Acetic Acid
Formic Acid (abt. 99%)

Package Size
1 mL×5A
50 mL
1 mL×5A
50 mL

4

for high-resolution MS (for QTofMS)
Absorbance

Gradient test*

Fluorescence
test

Particles

Suitability for
QTofMS analysis*The gradient test for QTofMS was conducted as a QTofMS-compatible test.

Since a high-performance liquid chromatograph-mass
spectrometer (LC-MS) has excellent characteristics, including thewidth of the scope of measuring objects and selectivity, it has
become popular in a wide range of fields. Mass spectrometerswith higher sensitivity and resolution have become popularizedthese days, and they are applied in research of metabolomics,proteomics, etc.
This product is a high-purity solvent optimal for measurements
using high-sensitivity and high-resolution MS✓ Testing requirement for compatibility with QTofMS• A wide mass range by full scan is guaranteed. (mass range: 50–3000)• Quality assurance with utilization of multivariate analysis-principal
component analysis.
✓ Gradient measurement by column connection (UHPLC measurement)✓ Particle assurance✓ Content and container that ensure that the solvent can be used inhigh-purity conditions at any time
• 500 mL or 1 L content optimal for using up• Glass bottles with special treatment that suppresses the elution of impurities
are used (Patent No. WO/2005/047884)

Low background noise

Company ACompany B

for QTofMS
Comparison of background noise between our solvent for QTofMS andLC/MS solvents of other companies (Companies A and B)

5

We reviewed the manufacturing processes
from various points so that it can be used inhigh-performance MS.
This product showed a lower backgroundnoise compared to the LC/MS solvents ofother companies (Companies A and B) in
quality confirmation using QTofMS.
[Total ion chromatogram]
Green: Company APurple: Company B
Red: for QTofMS (Fujifilm Wako)Small packaging for usability
Once opened, solvents become contaminated due to entry of air in laboratory, etc.
To prevent contamination after opening, this product comes in 500 mL or 1 L content
that can be used up.

These are the chromatograms immediately
after opening (green) and 1 week afteropening (red). Since it is highly likely thatthe increase in background noise would causeadverse effects on the measurement results inhigh-performance mass spectrometers, it isrecommended that the mobile phase is usedup each time.

1 week later

Immediately
after opening

Product list (for QTofMS)
Product Number018-26225
016-26221
130-18545
138-18541

Product Name
Acetonitrile
Methanol

164-27515

2-Propanol

212-01601

Ultrapure Water

Package Size
500 mL１L
500 mL1L
500 mL
1LConditions and equipment for data acquisition
Conditions for data acquisition
HPLC

ACQUITY UPLC H-Class (Waters)

ColumnWakopak® Ultra C18-2, 2.1mmφ×50mm

Column temperature 40℃±0.3℃
EluateAcetonitrile : Water [Gradient]

Detector

UV 210nm

Mass Spectrometer

Time-of-Flight Mass Spectrometer (Waters Xevo G2-XS)

Ionization

ESI

Mass Range

m/z 50～3000

Polarity

positive

Waters Xevo G2-XS
6

for GPC
Gradient test

AbsorbanceSuitability for
GPC analysis

GPC solvents are solvents for which it has been confirmed that there is no coloring under
heating for polymer dissolution or analysis. They are also guaranteed moisture, peroxides,
non-volatile substances or the less changes in refractive index or ultraviolet absorption
caused by impurities. This grades are optimal for preparation of GPC eluents.
Example of product specifications (1,1,1,3,3,3-Hexafluoro-2-propanol)
REQUIREMENTSPECIFICATION

Appearance

Colorless clear liquid

Density (20℃) (g/mL)

1.617-1.624

Absorbance

200 nmmax. 0.05

220 nmmax. 0.03

240 nmmax. 0.02

254-400 nm

max. 0.01

Suitability for GPC test

to pass test

Water (%)

max. 0.03

Residue after evaporation (%)

max. 0.001

Acidity (as HF) (%)

max. 0.001

Peroxides (as H2O2) (ppm)

max. 5

Assay (cGC) (%)

min. 99.5

Product list (for GPC)
Product Number
036-24481
032-24483
039-24493
043-33841
049-33843
045-33921
041-33923
046-33831
042-33833
048-33911
044-33913
082-10311
084-10315
134-18521
130-18523
205-20071
201-20073
209-20091
205-20093

７

Product Name
Chloroform
Chloroform, Amylene added
o-Dichlorobenzene
N,N-Dimethylacetamide
N,N-Dimethylformamide
Dimethyl Sulfoxide
1,1,1,3,3,3-Hexafluoro-2-propanol
1-Methyl-2-pyrrolidone
Tetrahydrofuran, Stabilizer Free
Tetrahydrofuran, with Stabilizer

Package Size
1L3L
3L
1L3L1L
3L
1L
3L
1L
3L
100 mL
500 mL
1L
3L
1L
3L
1L
3L

for the Japanese Pharmacopoeia General Tests
JP method

USP-NF method

EP method

The solvents for the Japanese Pharmacopoeia general tests (liquid chromatography) are HPLC
solvents for which compliance with the Japanese Pharmacopoeia (JP) reagent standards, theUnited States Pharmacopoeia (USP) reagent standards, and the European Pharmacopeia (EP)reagent standards is guaranteed, and for which some testing requirement of our company’s
own have been added.
Example of product specifications (Acetonitrile)

Example of product label

REQUIREMENT
Description

Indicates the expiration dateJP method（*added requirementby us)

USP-NF method

EP method

Colorless clear liquid Colorless clear liquid Colorless clear liquid

Color (APHA)-

max. 10-Density

0.780 - 0.783 g/ml*-

Sp. Gr. (20/20℃)
about 0.78

Refractive ｎD20

1.343～1.346*-

about 1.344

Absorbance

-

-

-

190 nm-max. 1.00-

200 nmmax. 0.07 (0.05*）-

max. 0.10

210 nmmax. 0.046 (0.03*）--

Indicates theguaranteed items*

220 nmmax. 0.027 (0.02 ）max. 0.05-

230 nmmax. 0.014 (0.01*）--

240 nm max. 0.009 (0.005*）--

254 nm-max. 0.01

250-280 nm-

max. 0.01-

255-420 nm--

max. 0.01--

max. 0.01

Gradient test

240-800 nm

to pass test*

to pass test-

Residue after
evaporation

0.001 ％*

0.005%以下-*

Titrable acid

Acidity 0.001 ％8 μeq/g以下

Titrable base-

0.6 μeq/g以下

-

Water

max. 0.05 ％*

0.003

-

pH (100g/L)--

Neutral to litmus
paperDistillation range(80-82 ℃)--

min. 95 %Ammonium (NH4)0.3 ppm*------*

Peroxides (H2O2)

5 ppm

Substances reducing
permanganate

to pass test**Fluorescence test

to pass test

Assay

min. 99.8 ％*--min. 99.5 ％min. 99.9 %Product list (Solvents for the Japanese Pharmacopoeia General Tests)
Product Number

Product Name019-21691
015-21693

1L
Acetonitrile

019-21696085-08711
081-08713

Hexane

1L
3L
1L

Methanol

136-15666
206-20981

3L
3 L×2

136-15661
132-15663

Package Size

3L
3 L×2

Tetrahydrofuran, Stabilizer Free

1L8Protective Jacket Dedicated to Gallon Glass bottle

GalloTect™

GalloTect™ is a protective jacket dedicated to gallon
glass bottle. FUJIFILM Wako has developed GalloTect™
with the desire to add safety to daily testing andresearch activities, based on user feedback such as"when lifted the gallon glass bottle, it smashed andcracked."

Body material：Polypropylene (PP)SIZE (mm)：W 175×D 175×H 231.5✔ Carry out solvent resistance test with 11 kinds of solvents!
Solvent resistance of body material has been confirmed for a week with various solvents.
Tested solvents: Acetone, Acetonitrile, Benzyl Alcohol, 1-Butanol, DMF, DMSO, Ethanol, Glycerin, Methanol, 2-Propanol, Water✔ A durability test was performed with a 10 kg weight!
Weight test was performed at 10 kg for 1 hour.
✔ The lid was opened and closed 200 times to confirm that there is no loosening!
✔ All-plastics not requiring waste separation!
A resin rivet was adopted for the screw portion.
When discarding, a troublesome separation is not necessary!
✔ Just fit your reagent shelf!
We focused on the design and eliminated the bulkinessthoroughly. The conventional reagent shelf can be stored gallon
glass bottle with GalloTect™.

How to use

1. Place the gallon bottle and set the lid.

Product Number
293-36321

2. Turn the lid in the direction of thearrow until you hear a click.

Product Name
Gallo Tect™

3. Compleate

Package Size
1

21Z00学01