Diabetes・Metabolism Related Research ReagentsContents
Product Line for Obesity / Diabetes
GLP-1 ELISA Kit Wako, High Sensitive

1

LBIS Mouse/Rat HMW Adiponectin ELISA Kit

1

Glucagon ELISA Kit Wako (Sandwich method)2

Mouse/Rat PYY ELISA Kit Wako

3

LH ELISA Kit

3

Insulin ELISA Kit

4

Mouse/Rat Proinsulin ELISA Kit

5

C-peptide（U-type）series

5

LBIS Human Apo B-48 ELISA Kit

6

LBIS Rabbit Apo B-48 ELISA Kit

6

Orexin A ELISA Kit Wako

7

YNT-185 Dihydrochloride Hydrate

7Albumin (Mouse,Rat) ELISA Kit

8

Turbidimetric Immuno Assay Kit (TIA Kit)

8

Product Line for Metabolism
LabAssay™ Series
LabAssay™ Ammonia

9

LabAssay™Triglyceride

10

LabAssay™Phospholipid

11

LabAssay™ALP

11

LabAssay™Cholesterol

12

LabAssay™NEFA

12

LabAssay™Creatinine

13

LabAssay™Glucose

13

Compounds for Research of Diabetes
・Sulfonylureas compounds

14

GLP-1 ELISA Kit
GLP-1 is the gastrointestinal peptide hormone secreted from intestinal L cells, and shows the hypoglycemic action throughthe enhancement of insulin secretion and the suppression of glucagon secretion by the sugar stimulus. The report has
been issued that GLP-1 is useful for the treatment of type 2 diabetes as the new therapeutic agent for diabetes.

GLP-1 ELISA Kit Wako, High Sensitive（Total GLP-1assay）
GLP-1 ELISA Kit Wako ,High Sensitive, is a sandwich ELISA system for quantitative measurement of mouse/rat GLP-1.
[Kit Contents]
Amount1 plate1 bottle
60 mL/1 bottle
100 µL/1 vial
100 µL/1 vial
12 mL/1 bottle
12 mL/1 bottle
100 mL/1 bottle
4 sheets3

Abs. 450-620nm

1
2
3
4
5
6
7
8
9

[Standard Curve]

Components
Antibody-coated Plate
GLP-1 Standard
Buffer Solution
Biotinylated Anti GLP-1 Antibody Solution
Peroxidase-conjugated Streptavidin Solution
TMB Solution
Stop Solution
Wash Solution (x10)Plate Seal

2.5
2
1.5
1
0.5
0
0

[Performance]

10

20

30

GLP-1 (pmol/L)・Concentration of the standard solution : 0.94-30 pmol/L・Dynamic range : 4.7-150 pmol/L *Case of 10 μL
・Sample volume : 10 μL
・Measure time : 4 hour・Cross-reactivity : Mouse, Rat[References]
1) Harada, K. et al.: Biochem. Biophys. Res. Commun., 501, 1009(2018)2) Io, F. et al.: Eur. J. Pharmacol., 853, 136(2019)3) Harada, K. et al.: Biochem. Biophys. Res. Commun., 500, 723(2018)

Product Code299-75501

Product
GLP-1 ELISA Kit Wako, High Sensitive

Grade

Pack

for Diabetes Research

96 tests

Adiponectin ELISA Kit
LBIS Mouse/Rat HMW Adiponectin ELISA Kit
Adiponectin is one of cytokines secreted from adipocyte (adipocytokines, adipokines), and controls lipid metabolism andinsulin sensitivity. Adiponectin is an important substance showing anti-diabetic, anti-atherosclerotic, and anti-inflammatory
actions.
Mouse/Rat HMW Adiponectin ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse or rat High
Molecular Weight Adiponectin.
[Cross-reactivity][Kit Contents]
1
2
3
4
6
7
8
9

Antibody-coated Plate
Standard Adiponectin (2,000 ng/mL)
Buffer Solution
HRP-labeled anti-adiponectin antibody
TMB Solution
Stop Solution
Wash Solution (x10)Plate Seal

1 plate
200 μL/1 vial
60 mL/1 bottle
100 µL/1 vial
12 mL/1 bottle
12 mL/1 bottle
100 mL/1 bottle
3 sheets

Name

Mouse

Rat

Adiponectin (HMW)100

Adiponectin (Hexamer)<5

Adiponectin (Trimer)-

Adiponectin (Monomer)-

Adiponectin (HMW)100

Adiponectin (Monomer)-[Performance][References]

・Assay range：3.13～200 ng/mL
・Test Time：4 hours
・Sample volume：10 μL
・Samples：Mouse/Rat Serum, Plasma

1) Kim, TH. et al.: Eur. J. Pharmacol., 830, 95(2018)
2) Watanabe, K. et al.: Obes. Surg., 28, 1532(2018)3) Tobita, H. et al.: Mol. Med. Rep., 17, 6840(2018)

Product CodeMaker No.

634-13071

AKMAN-011

Product
LBIS Mouse/Rat HMW Adiponectin ELISA Kit

1

Cross-reactivity
（%）

Pack
96 tests

Glucagon ELISA Kit
Glucagon is a peptide hormone comprising 29 amino acids and secreted from α cells in the islets of Langerhans.
Physiologically, it acts on liver to degrade glycogen to glucose and increase blood glucose level. Together with insulin, it
plays an important role in maintaining blood glucose level.

Glucagon ELISA Kit Wako (Sandwich method)
This kit uses sandwich ELISA with a monoclonal antibody that recognizes N-terminal and a monoclonal antibody thatrecognizes C-terminal of glucagon. The assay with this kit shows little cross-reactivity, if any, to glucagon-related peptides
such as glicentin, oxyntomodulin, GLP-1 or GLP-2.
[Cross-reactivity]

Glucagon and glucagon-like peptide

Name
Glicentin(1-69) (human)

0.68

Glicentin(1-69) (rat)

0.96

Glicentin(1-69) (mouse)

0.97

Glicentin(1-61) (human)

0.95

Glicentin(1-61) (rat)not confirmed

Glicentin(1-61) (mouse)

not confirmed

Oxyntomodulin (human, rat and mouse)

0.64

Mini-glucagon (human, rat and mouse)

N.D.

[Standard Curve][Kit Contents]
1 Antibody-coated Plate
2 Glucagon Standard
3
4
5
6
7
8

Cross-reactivity（%）

HRP conjugated Anti Glucagon Antibody Solution
TMB Solution
Stop Solution
Buffer
Wash Solution (x20)
Plate Seal

1 plate0.287 pmoL
/ 1 vial
12 mL/1 bottle
12 mL/1 bottle
12 mL/1 bottle
12 mL/1 bottle
50 mL/1 bottle
2 sheets[Performance]
・Assay range：7.8～500 pg/mL・Test Time：20 hours
・Sample volume：10 μL
・Samples：Human/Mouse/Rat Serum, Plasma, Cell culture medium

Product Code292-80001

Product
Glucagon ELISA Kit Wako (Sandwich method)

2

Grade

Pack

for Obesity Research

96 tests

PYY ELISA Kit
PYY is a gastrointestinal peptide hormone consisting of 36 amino acid residues. It is mainly released from endocrine cells
localizing in the intestinal tract after food ingestion, and inhibits appetite through the vagus nerve and blood.

Mouse/Rat PYY ELISA Kit Wako
This ELISA kit is used for the quantitative determination of mouse and rat PYY in plasma and serum samples.
[Performance]
・Assay range：0.15～12.5 ng/mL ・Test Time：20 hours
・Sample volume：25 μL
・Samples：Mouse/Rat Serum, Plasma[Standard Curve]

1 Antibody-coated Plate
2 Mouse/Rat PYY Standard
3 Biotinylated Mouse/Rat PYY
4 Anti Mouse/Rat PYY , Rabbit
5 HRP Labeled Streptavidin Solution
6 Enzyme Substrate Solution
7 Stop Solution
8 Buffer
9 Wash Solution (concentrated)
10 Plate Seal

1 plate12.5 ng/ 1 vial
1 vial8.5 mL/1 bottle
12 mL/1 bottle
12 mL/1 bottle
12 mL/1 bottle
25 mL/1 bottle
50 mL/1 bottle
3 sheets

2.0
Absorbance(450nm)[Kit Contents]

1.5
1.0
0.5
0.0
0.1

[References]

1

10

100

PYY (ng/mL)

1) Watanabe, K. et al.: PLoS One., 13, e0202083(2018)
2) Nakatani, A. et al.: Biochem. Biophys. Res. Commun., 501, 955(2018)
3) Miyamoto, J. et al.: PLoS One., 13, e0196579(2018)

Product Code

Product

291-73501

Mouse/Rat PYY ELISA Kit Wako

Grade

Pack

for Obesity Research

96 tests

LH ELISA Kit
LH Rat ELISA Kit
LH(Luteinizing Hormone) is produced and stored in basophilic cells called gonadotrophs in the anterior pituitary gland. LH
is found in all vertebrates from fishes to mammals. Some reports indicated the presence of LH-like substance in the testis.
Rat LH ELISA Kit (S-type) is a sandwich ELISA system for quantitative measurement of rat LH.
[Performance]
・Assay range：1.565 ～ 50 ng/mL
・Test Time：4 hours
・Sample volume：10 μL
・Samples：Rat Serum, Plasma[References]
1) Satou, K. et al.: J. Reprod. Dev., 63, 199(2017)
2) Takeda, T. et al.: J. Toxicol. Sci., 42, 13(2017)3) Tomori, Y. et al.: Neurosci. Res., 117, 35(2016)

Product CodeMaker No.

636-23921

AKRLH-010S

Product
LBIS Rat LH ELISA Kit (S-type)3

Pack
96 testsInsulin ELISA Kit
Insulin is a peptide hormone secreted from B cells of islet of Langerhans in the pancreas with a molecular weight of about
5800 and pI 5.4. It is consisted of 2 chains, A and B. It has 3 disulfide bonds formed between A6 and A11, A7 and B7, andA20 and B19.
FUJIFILM Wako Shibayagi Corporation offers a variety of kits for the measurement of insulin for your purposes. Select a kitthat fits your researches.

Type

U typeU-E type

S type

RTU type

T typeH typeAnimal

Mouse

Rat

Mouse

Rat

Mouse

Rat

Mouse

Rat

Mouse

Rat

Test time

3 hours

3 hours

3 hours

2h 50m2h 50m2h 50m3 hours

3 hours

3 hours

3 hours

Concentrati
on(pg/mL)200,000

Assay
Range

●

●

100,000●●

12,000

●

●

●

●

●

●

●

●

●

10,000

●

●

●

●

●

●

●

●

5,000

●

●

●

●

●

●

●

●

●

●

2,500

●

●

●

●

●

●

●

●

●

●

500

●

●

●

●

●

●

●

●

●

●

●

●

●

●

●

●

●

●

●

156
100
78
39
30

■U type, U-E type: High sensitivity type. Suitable for low insulin concentration like after fasting period.
■S type: Reduce cross-reaction with proinsulin, specific detect of insulin.
■RTU type: Contains ６ concentration standard, antibody solution, POD・Avidin solution that are diluted and ready to use.
■T type: Normal type (standard kit for insulin analysis).
■H type: For analysis of highly concentrated insulin sample.

Product CodeMaker No.

633-03411

AKRIN-031

636-05581

Product

Assay Range

Package

LBIS Mouse Insulin ELISA Kit (U-type)39-2,500 pg/mL

96 tests

AKRIN-130

LBIS Rat Insulin ELISA Kit (U-E type)39-2,500 pg/mL

96 tests636-07281

AKRIN-011S

LBIS Mouse Insulin ELISA Kit (S-type)78-5,000 pg/mL

96 tests637-07191

AKRIN-010S

LBIS Rat Insulin ELISA Kit (S-type)0.1-10 ng/mL

96 tests639-23911

AKRIN-011RU

LBIS Mouse Insulin ELISA Kit(RTU)

100-12,000 pg/mL

96 tests636-24141

AKRIN-010RU

LBIS Rat Insulin ELISA Kit(RTU)

100-12,000 pg/mL

96 tests634-01481

AKRIN-011T

LBIS Mouse Insulin ELISA Kit

0.156-10 ng/mL

96 tests637-01471

AKRIN-010T

LBIS Rat Insulin ELISA Kit

0.156-10 ng/mL

96 tests630-10371

AKRIN-011H

LBIS Mouse Insulin ELISA Kit(H-type)0.5-100 ng/mL

96 tests633-10621

AKRIN-010H

LBIS Rat Insulin ELISA Kit(H-type)0.5-100 ng/mL

96 tests4

Proinsulin ELISA Kit
Proinsulin, known as a precursor of insulin, is produced in B-cells of islet of Langerhans in the pancreas.

Mouse/Rat Proinsulin ELISA Kit
Mouse/Rat Proinsulin ELISA Kit is a sandwich ELISA system for quantitative measurement of total mouse/rat proinsulin.
[Performance]
・Assay range：7.35～471.5 pg/mL・Test Time：5 hours
・Sample volume：10 μL
・Samples：Mouse/Rat Serum, Plasma[Cross-reactivity][Kit Contents]

Sample

1
2
3
4
5
6
7

Antibody-coated Plate
1 plateGLP-1 Standard
1 bottle
Buffer Solution
60 mL/1 bottle
Biotinylated Anti GLP-1 Antibody Solution 100 µL/1 vial
HRP-conjugated Streptavidin
100 µL/1 vial
TMB Solution
12 mL/1 bottle
Stop Solution
12 mL/1 bottle
100 mL
8 Wash Solution (x10)/1 bottle
9 Plate Seal
4 sheets

Mouse

Rat

[References]
1) Matsunaga, K. et al.: J. Cell. Sci., 130, 541(2017)2) Nishi, K. et al.: Diabetes., 65, 3015(2016)
3) Machida, M. et al.: Biol. Pharm. Bull., 38, 587(2015)

Product CodeMaker No.

636-23041

AKMPI-111

Substance

Cross reaction(%)

Proinsulin

100

Insulin－C-peptide－

Leptin－Proinsulin

100

Insulin－C-peptide－

Leptin－Proinsulin＋

Insulin－C-peptide

－

Human

Product
LBIS Mouse/Rat Proinsulin ELISA Kit

Pack
60 tests

C-peptide ELISA Kit
C-peptide is released into the blood in approximately equimolar to insulin by the processing of proinsulin, a precursor of
insulin. By the measurement of C-peptide concentration in the blood, it is possible to know the insulin secretory potency of
pancreatic β cells in the case during insulin administration or insulin antibodies existing.

C-peptide（U-type）seriesThis product is ELISA kit measurable specifically of C-peptide in the mouse or rat specimen.
[Performance]
・Assay range：46.9～3,000 pg/mL・Test Time：5 hours
・Sample volume：10 μL
・Samples：Mouse/Rat Serum, Plasma[References]
Mouse(U-type) (AKRCP-031)
1) Motoyama, H. et al.: PLoS One., 13, e0197175(2018)
2) Joo, E. et al.: Diabetes., 66, 868(2017)
Rat(U-type) (AKRCP-030)
1) Park, JH. et al.: J. Ethnopharmacol., 234, 172(2019)2) Park, C. et al.: Phytother. Res., 32, 2541(2018)3) Niibo, M. et al.: J. Dairy. Sci., 102, 997(2018)

Product CodeMaker No.

Product

631-07231

AKRCP-031

RBIS Mouse C-peptide ELISA Kit (U-type)

96 tests639-07271

AKRCP-030

RBIS Rat C-peptide ELISA Kit (U-type)

96 tests5

PackApolipoprotein B-48
Apolipoprotein B-48 is a specific structural protein in lipoproteins derived from the small intestine, such as chylomicron(CM) and chylomicron remnants. Since one role of CM is to transfer exogenous lipids derived from food to the liver or
peripheral tissues, the measurement of Apo B-48 is considered a best marker for observation of exogenous lipid
transportation after food intake.

LBIS Human Apo B-48 ELISA Kit[Performance]
・Assay range：2.5 ～ 160 ng/mL
・Test Time：2 hours 50 min
・Sample volume：50 μL(diluted)
・Samples：Human Serum,
Plasma[References][Kit Contents]
1
2
3
4
5
6
7
8
9

1) Blom,D. et al.: J Clin Lipidol., 12,1234(2018)2) Drouin-Chartier,J. et al.: J Lipid Res., 59,1501(2018)
3) Bjornshave,A. et al.: Eur J Nutr., 58,755(2018)
4) Schioldan,A. et al.: Eur J Nutr., 57,795(2018)
5) Bjornshave,A. et al.: Nutrients., 10,E122(2018)

Product CodeMaker No.

637-10641

AKHB48Anti-apoB-48 coated Plate
Standard human apo B-48
Buffer Solution
Biotinylated Anti Apo B-48 Antibody Solution
HRP-conjugated Streptavidin
TMB Solution
Stop Solution
Wash Solution (x10)Plate Seal

Product
LBIS Human Apo B-48 ELISA Kit

1 plate1 vial
60 mL/1 bottle
100 µL/1 vial
100 µL/1 vial
12 mL/1 bottle
12 mL/1 bottle
100 mL/1 bottle
4 sheetsPack
96 tests

LBIS Rabbit Apo B-48 ELISA Kit[Performance]
・Assay range：19.5 ～ 1250 ng/mL
・Test Time：2 hours 50 min
・Sample volume：50 μL(diluted)
・Samples：Rabbit Serum, Plasma

Product CodeMaker No.

628-04901

AKRB48[Kit Contents]
1
2
3
4
5
6
7
8
9

Anti-apo B-48 coated Plate
Standard rabbit apo B-48
Buffer Solution
Biotinylated Anti Apo B-48 Antibody
HRP-conjugated Streptavidin
TMB Solution
Stop Solution
Wash Solution (x10)Plate Seal

Product
LBIS Rabbit Apo B-48 ELISA Kit

6

1 plate1 vial
60 mL/1 bottle
100 µL/1 vial
100 µL/1 vial
12 mL/1 bottle
12 mL/1 bottle
100 mL/1 bottle
4 sheetsPack
96 testsOrexin A ELISA Kit
Orexin A ELISA Kit Wako
Given that the level of orexin A in the cerebrospinal fluid is significantly decreased in patients with narcolepsy, a type ofsleep disorder, orexin A is thought to play an important role in controlling sleep functions. The level of orexin A has beenmeasured conventionally using radioimmunoassay. However, due to the need for radioisotopes (RI), the measurements
could only be performed at dedicated institutions with proper equipment.
This product is an ELISA kit that enables simple measurements of orexin A from human cerebrospinal fluid, as well as rat
cerebrospinal fluid, serum, and plasma without the need for radioisotope.

[Performance][Standard Curve]

・Assay range：4.69 ～ 300 pg/mL・Test Time：About 20 hours
・Sample volume：25 μL
・Samples：human cerebrospinal fluid, rat cerebrospinal fluid,
rat plasma, rat serum[Reference]
Ono, T., et al.: Psychiatry Clin Neurosci., 72, 11, 849 (2018).

Product Code293-79801

Product
Orexin A ELISA Kit Wako

Grade

Pack

for Immunochemistry

96 tests

YNT-185 Dihydrochloride Hydrate
YNT-185·2HCl is non-peptide orexin receptor 2 (OX2R) agonist. Orexins are a family of neuropeptides, which are secreted
by orexin-producingnerve present in the hypothalamus. There are two variants, orexin receptors exist as two types ofreceptors, type 1 and type 2. In particular, the orexin 2 receptor is involved in sleep-wake regulation, and orexin deficiency
in the brain causes narcolepsy, a sleep disorder manifesting as an unbearable sleepiness during the day. Although it has
been reported that intraventricular administration of orexin leads to symptom alleviation in narcolepsy mouse models,
therapeutic effects cannot be expected unless it is administered into the brain, due to orexin being a peptide and being
unable to pass through the blood-brain barrier. Intraventricular or intraperitoneal administration of YNT-185·2HCl has beenshown to extend wake time, and has also been reported to improve the symptoms of narcolepsy mouse models.
[structural formula][References]
1) Nagahara, T., et al.: J. Med. Chem., 58, 7931 (2015).
2) Irukayama-Tomobe, Y., et al.: Proc. Natl. Acad. Sci. USA, 114(22), 573(2017).
3) Toyama, S., et al.: Anesthesiology, 128, 992 (2018).
4) Yamamoto, K., et al.: Naunyn Schmiedebergs Arch. Pharmacol. (2019). doi: 10.1007/s00210-019-01646-x.

Product Code

Product

254-00641

YNT-185 Dihydrochloride Hydrate

7

Grade

Pack

for Cellbiology

5mg

Albumin ELISA Kit
FUJIFILM Wako Shibayagi provides a unique lineup of ELISA kits for evaluation of urine, serum and plasma albuminfor rat and mouse model organism. For high throughput analysis TIA reagents for the application on chlinicalchemistry auto-analyser are available.

Albumin (Mouse, Rat) ELISA Kit[Performance]
・Assay range：50 ～ 1,000 ng/mL
・Test Time：2 hours 20 min
・Sample volume：5 μL(diluted)
・Samples：Mouse/Rat Serum, Plasma[References]
Albumin Mouse ELISA Kit
1) Mizukami, K. et al.: Naunyn. Schmiedebergs. Arch. Pharmacol.,391, 1319(2018)
2) Rhee, H. et al.: Kidney. Res. Clin. Pract., 37, 222(2018)
3) Ishikawa, H. et al.: Biosci. Biotechnol. Biochem., 82, 515(2018)

Albumin Rat ELISA Kit
1) Kanno, M. et al.: Nephrology., 23, 1046(2018)
2) Ishibashi, Y. et al.: Horm. Metab., 48, 613(2016)
3) Takeshige, Y. et al.: Hypertens., 39, 415(2016)

Product CodeMaker No.

Product

Pack

634-04301

AKRAL-121

LBIS Mouse Albumin ELISA Kit

96 tests631-04311

AKRAL-120

LBIS Rat Albumin ELISA Kit

96 tests

Turbidimetric Immuno Assay Kit (TIA Kit)
[Performance][Assay procedure]・Assay range：6.17 ～ 500 μg/mL
・Test Time：10 min
・Sample volume：6 μL
・Samples：Mouse/Rat Urine, Plasma

For Hitachi 7180 auto-analyzer*Note that operation of auto-analyzer is different depending on
the type of machines[References]
LBIS Mouse Urinary Albumin Assay Kit
1) Haku, S. et al.: Biomed. Res. Int., 2018, 2817045(2018)2) Iwashita, Y. et al.: Am. J. Physiol. Renal. Physiol., 310,
F1206(2016)
3) Zhu, K. et al.: Free. Radic. Biol. Med., 83, 21(2015)
LBIS Rat Urinary Albumin Assay Kit
1) Tatsuyori, M. et al.: Eur. J. Pharmacol., 756, 85(2015)
2) Ruixia, M. et al.: Int. J. Clin. Exp. Pathol., 8, 14063(2015)3) Sakai, M. et al.: Eur. J. Pharmacol., 761, 109(2015)

Product CodeMaker No.

Product

638-25561

AKRAL-021S

LBIS Mouse Urinary Albumin Assay Kit (S-type)

60 tests634-25301

AKRAL-020S

LBIS Rat Urinary Albumin Assay Kit (S-type)

60 tests

8

Pack

LabAssay™ Series
The LabAssay™ Series is a collection of biochemical assay kits for analyzing samples from humans, mice, and otheranimals. Since analyses are performed in microtiter plates, many samples can be concurrently evaluated, and only small
sample amounts are needed.

LabAssay™ Overview
Code No.

Maker No.

633-51761

LABNH3-M1 Ammonia nitrogen633-51021

LABALP-M1

ALP

635-50981

LABCHO-M1

Cholesterol636-51011 LABCREA-M1

Creatinine

638-50971 LABGLUC-M1

Glucose633-52001 LABNEFA-M1

NEFA

639-51001

Measuring factor

LABPLIP-M1

Phospholipid

632-50991 LABTRIG-M1

Triglyceride

Animalspecies

SampleStandard curve range

SampleamountMeasuring time

Pkg size

Serum, Plasma,
Culture supernatantSerum, Plasma,
Culture supernatantSerum, Plasma,
Culture supernatant

100～400 μg/dL

70μL

approx. 70 min

700 tests

0.0625～0.5 mmol/L

20μL

approx. 20 min

500 tests

50～592.2 mg/dL

2μL

approx. 10 min

500 tests

2.5～10 mg/dL

50μL

approx. 40 min

500 tests

50～500 mg/dL

2μL

approx. 10 min

500 tests

0.5～1.97 mEq/L※Oleic acid 1mEq=1mmol

4μL

approx. 20 min

500 tests

150～596.1 mg/dL

2μL

approx. 10 min

500 tests

100～888 mg/dL

2μL

approx. 10 min

350 tests

Human,
Mouse, Serum, Plasma, Urine
Rat,
Serum, Plasma,
Dog,
Culture supernatant
Cat
Serum, Plasma,
Culture supernatantSerum, Plasma,
Culture supernatantSerum, Plasma,
Culture supernatant

LabAssay™ Ammonia
Ammonia is mainly produced in the intestine or the kidney. Ammonia produced in the body is converted to urea
through a series of reactions known as the urea cycle in the liver and eliminated through urine.
Assay Principle (Modified Fujii-Okuda Method)
Ammonia is converted to Dioxydiphenylamine by the reaction of Phenol and Sodium Pentacyanonitrosyl ferrate(III). A
reaction of the Dioxydiphenylamine with Sodium Hypochlorite produces Indophenol, which pigments blue, under alkalineconditions. LabAssay™ Ammonia is a kit used for the quantitative determination of ammonia nitrogen in samples bymeasuring absorbance of the blue color.

[Kit Contents]
1

Deproteinizing

100 mL×1vial

2

Chromogen Reagent A

50 mL×1vial

3

Chromogen Reagent B

25 mL×1vial

4

Chromogen Reagent C

50 mL×1vial

5

Ammonia Standard Solution

15 mL×1vial

6

Dilute olution for Standard

20 mL×1vial[Performance]
・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength

Abs. (630nm)[Standard Curve]100~400 μg/dL(μg/100mL)
approx. 70 minutes
70 μL
630 nmAmmonia nitrogen (μg/dL: μg/100mL)[Reference]
1) Inokuma, K. et al.: Microb. Cell Fact., 17, 153 (2018).

Code No.
633-51761

Maker No.

Product name

Package size

Storage Condition

LABNH3-M1

TM

700 tests

Keep at 2-10℃

LabAssayAmmonia9

LabAssay™ Triglyceride
Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides,
cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

Assay Principle
Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is
produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative
condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine.
LabAssay™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue
color which is generated by the oxidative condensation reaction.
[Standard Curve][Kit Contents]
1 Buffer

105 mL×3vials

2 Chromogen Substrate
3 Standard Solution

for 105 mL×3vials
10 mL×1vial[Performance]
・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength

100~888 mg/dL(mg/100mL)
approx. 10 minutes
2 μL
600 nm(Main), 700 nm(Sub)
Triglyceride (mg/dL : mg/100mL)[References]
1) Gao, F. et al. :Evid. Based Complement. Alternat. Med., 2015, 801291(2015). ※Extraction liquid of rat kidney
2) Funakoshi, T. et al. : Biochem. Biophys. Rep., 13, 39(2018). ※Rat primary muscle satellite cell3) Moser, V. A. and Pike, C. J. : eNeuro, 4, e0077-17(2017). ※Mouse plasma4) Fujii, N. et al. : Aging Cell, 16, 508(2017). ※Rat plasma5) Oh, T-W., et al. : J. Exerc. Nutrition Biochem., 19, 247(2015). ※Rat plasma
6) Fan, Y. et al. : J. Biomed. Sci., 23, 56(2016). ※Mouse serum

Code No.
632-50991

Maker No.
LABTRIG-M1

Product name
TM

LabAssay

Triglyceride

10

Package size

Storage Condition

350 tests

Keep at 2-10℃

LabAssay™ Phospholipid
Phospholipids are known as not only as major component of cell membranes but also perform vital functions within the bodysuch as emulsification and absorption of fats or coagulation of blood.
Assay Principle (Choline Oxidase・DAOS method)
Phospholipids are hydrolyzed by Phospholipase D to release hydrogen peroxide. The formed hydrogen peroxide promotes
oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt（DAOS) with 4aminoantipyrine. LabAssay™ Phospholipid is a kit to determine Phospholipid concentration in samples by measuring
absorbance of a blue color which is generated by the oxidative condensation reaction.
[Kit Contents][Standard Curve]

1 Buffer

50 mL×8vials

2 Chromogen Substrate
3 Standard Solution

for 50 mL×8vials
10 mL×2vials[Performance]
・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength150~596.1 mg/dL(mg/100mL)
approx. 10 minutes
2 μL
600 nm(Main), 700 nm(Sub)
Phospholipid (mg/dL・mg/100mL)[References]
1) Tatematsu, Y. et al. : Biol. Pharma. Bull., 41, 319(2018). ※Liposome
2) Kuge, H. et al.: J. Biol. Chem., 289, 26783 (2014). ※Liposome
3) Kessler, E. C. et al.: J. Dairy. Sci., 97, 5481 (2014). ※Bovine
4) Xu, Q. et al.: Biosci. Biotechnol, Biochem., 77, 1390 (2013). ※Extraction liquid of mouse kidney

Code No.

Maker No.

Product name

Package size

Storage Condition639-51001

LABPLIP-M1

LabAssayTM Phospholipid

500 tests

Keep at 2-10℃※Not available for sale in the US.

LabAssay™ ALP
Alkaline Phosphatase (ALP) is an enzyme distributed in a variety of tissues such as the liver, bone and small intestines inanimals. Especially, it is used one of the Osteogenesis Markers in bone metabolism research area.
Assay principle (Alkaline Phosphatase activity assay with p-Nitrophenyl Phosphate as a substrate)
p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol in the presence of Alkaline Phosphatase(ALP). LabAssay™ ALP
is a kit designed to determine Alkaline Phosphatase activity in samples by measuring the amount of p-Nitrophenol
released by p-Nitrophenylphosphate as a phosphatase substrate.
[Standard Curve][Kit Contents]
1 Substrate Tablet

20 tablets

2 Buffer Solution

100 mL×1vial

3 Stop Solution

100 mL×1vial

4 Standard Solution

10 mL×1vial[Performance]

・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength

0.0625～0.5 mmol/L
approx. 20 minutes
20 μL
405 nmp-Nitrophenol(mmol/L)[References]1) Ito, S. et al. : J. Pharmacol. Exp. Ther., 333, 341(2010). ※Extraction liqiud of mouse kidney
2) Matsuyama, A. et al. : Clin. Exp. Pharmacol. Physiol., 45, 75(2018). ※MC3T3-E1cell, C2C12Ccell
3) Chiba, T. et al. : J. Atheroscler. Thromb, 23, 1099(2016). ※Mouse plasma4) Kohno, Y. et al. : Stem Cell Res. Ther., 8, 115(2017). ※Mesenchymal stem cell

Code No.
633-51021

Maker No.
LABALP-M1

Product name
TM

LabAssay

11

ALP

Package size

Storage Condition

500 tests

Keep at 2-10℃

LabAssay™ Cholesterol
Cholesterol, a major component of cell membranes and the starting material in steroid synthesis in many animals, is afactor behind arteriosclerosis and other vascular diseases.

Assay Principle (Cholesterol Oxidase・DAOS method)
Hydrogen peroxide produced by a reaction of cholesterol and cholesterol oxidase let N-Ethyl-N-(2-hydroxy-3-sulfopropyl)3,5-dimethoxyaniline sodium salt（DAOS）and 4-Aminoantipyrin oxidize and condensate. LabAssay™ Cholesterol is a kit
to determine total cholesterol by measuring absorbance of the blue color which is generated by the oxidative condensation
reaction.
[Kit Contents][Standard Curve]
1 Buffer

150 mL×2vials

2 Chromogen

150 mL×2vials

3 Standard Solution

10 mL×1vial[Performance]
・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength50~592.2 mg/dL(mg/100mL)
approx. 10 minutes
2 μL
600 nm(Main), 700 nm(Sub)

Cholesterol (mg/dL : mg/100mL)[References]
1) Kobayashi, Y. et al. : J. Pharmacogn. Nat. Prod., online(2015) doi: 10.4172/2472-0992.1000113※Extraction liquid of mouse kidney
2) Gao, F. et al. :Evid. Based Complement. Alternat. Med., 2015, 801291(2015). ※Extraction liquid of rat kidney
3) Yoshioka, H. and Onosaka, S. : Fundam. Toxicol. Sci., 3, 151(2016). ※Mouse plasma4) Fujii, N. et al. : Aging Cell, 16, 508(2017). ※Rat plasma

Code No.
635-50981

Maker No.

Product name

Package size

Storage Condition

LABCHO-M1

TM

500 tests

Keep at 2-10℃

LabAssay

Cholesterol※Not available for sale in the US.

LabAssay™ NEFA
NEFA (Non-esterified fatty acid) in the blood is transported complexed with an albumin to peripheral tissues. They areimportant sources of fuel for the peripheral tissues. The concentration of NEFA in the blood is regulated by a release fromthe adipose tissues, a consumption in the periphral tissues or a take up from the liver.

Assay Principle (ACS・ACOD method)
NEFA (Non-esterified fatty acid) forms Acyl-CoA in the presence of Acyl-CoA synthetase(ACS). Hydrogen peroxide, which
is produced by a reaction between the Acyl-CoA and Acyl-CoA oxidase(ACOD), promotes oxidative condensation of 3methyl-N-ethyl-N-(β-hydroxyethyl)-aniline（MEHA） with 4-aminoantipyrine. LabAssay™ NEFA can be used for thequantitative determination of NEFA in samples by measuring absorbance of a blue purple color which is generated by theoxidative condensation reaction.

[Kit Contents][Standard Curve]

1
2
3
4

Chromogen Reagent A
Solvent A
Chromogen Reagent B
Solvent B
NEFA Standard Solution
5（Oleic acid 1 mEq/L）[Performance]

for 10 mL×6vials
65 mL×1vial
for 20 mL×6vials
130 mL×1vial
10 mL×1vial※Oleic acid 1mEq=1mmol

・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength

0.5~1.97 mEq/Lapprox. 20 minutes
4 μL
550 nm

NEFA (mEq/L)[References]
1) Kobayashi, Y. et al. : J. Pharmacogn. Nat. Prod., online(2015) doi: 10.4172/2472-0992.1000113 ※Extraction liquid of mouse kidney
2) Gao, F. et al. :Evid. Based Complement. Alternat. Med., 2015, 801291(2015). ※Extraction liquid of rat kidney
3) Ogawa, K. et al. : Reprod. Med. Biol., online(2018). doi.org/10.1002/rmb2.12084 ※Follicular fluid derived from pig vesicular ovarian follicle4) Wang, F. et al.: J. Mol. Endocrinol., 52, 133 (2014). ※Mouse plasma

Code No.

Maker No.

633-52001

LABNEFA-M1

Product name
LabAssayTM NEFA

※Not available for sale in the US and Europe.

12

Package size

Storage Condition

500 tests

Keep at 2-10℃

LabAssay™ Creatinine
Creatinine is a metabolite which is produced by creatinine phosphate directly or dehydration of creatinine in muscle andnerve. It is excreted from the body through kidney glomerular filtration.

Assay Principle (Jaffé method)
LabAssay™ Creatinine can be used to measure the creatinine levels in samples by Jaffe's reaction where creatinine
produces quantitatively an orange color with picric acid in alkaline condition.
[Standard Curve][Kit Contents]
1 Depoteinizin Reagent

150 mL×2vials

2 Picric Acid Reagent

for 150 mL×2vials

3 0.75mol/L Sodium Hydroxide Solution

10 mL×1vial

4 Standard Solution

10 mL×1vial[Performance]

・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength

2.5~10 mg/dL(mg/100mL)
approx. 40 minutes
50 μL
520 nmCreatinine (mg/dL: mg/100mL)[References]
1) Kawamoto, T. et al. : Glycative Stress Res., 3, 15(2016). ※Human urine2) Guan, Y. et al. : J. Pharmacol. Sci., 135, 81(2017). ※Mouse urine、Mouse plasma3) Ito, K. et al. : Biol. Pharm. Bull., 38, 1169(2015). ※Rat urine、Rat plasma4) Tahara, Y. et al. : Med. Chem. commun., 8, 415(2017). ※Mouse serum

Code No.

Maker No.

Product name

Package size

Storage Condition

636-51011

LABCREA-M1

LabAssayTM Creatinine

500 tests

Keep at 2-10℃※Not available for sale in Europe.

LabAssay™ Glucose
Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism.
Glucose converges to a stable ratio of α-form and β-form in solutions.

Assay Principle (Mutarotase・GOD method)
α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between
β-D-Glucose and glucose oxidase(GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine
quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples
by measuring absorbance of a red color which is generated by the oxidative condensation reaction.

[Standard Curve][Kit Contents]
1 Buffer

150 mL×2vial

2 Chromogen Reagent

for 150 mL×2vials

3 Glucose StandardⅠ

10 mL×1vial

4 Glucose StandardⅡ

10 mL×1vial[Performance]

・ Standard curve range
・ Measurement time・ Amount of sample
・ Measurement wavelength

2.5~10 mg/dL(mg/100mL)
approx. 40 minutes
50 μL
505 nm(Main), 600 nm(Sub)

Glucose (mg/dL: mg/100mL)[References]
1) Yamashita, Y. et al.: Biosci. Biotechnol. Biochem., 77, 888 (2013). ※Mouse plasma2) Narita, T. et al. : Exp. Gerontol., 104, 127(2018). ※Rat plasma
3) Yamasaki, M. et al. : Food Sci. Technol. Res., 21, 827(2015). ※Mouse serum
4) Fan, Y. et al. : J. Biomed. Sci., 23, 56(2016). ※Mouse serum

Code No.
638-50971

Maker No.

Product name

Package size

Storage Condition

LABGLUC-M1

TM

500 tests

Keep at 2-10℃

LabAssay

Glucose13

Compounds for Research of Diabetes-Sulfonylureas compoundsProduct nameWako CodePackage Size

Chlorpropamide032-21482

25g

for Pharmacology Research

Product name
Glibenclamide
for Biochemistry

CAS No. 94-20-2
C10H13ClN2O3S=276.74[Specification Assay] 97.0+% (HPLC)[Solubility] Methanol[Overview] Sulfonylurea (SU) compounds
act by enhancing insulin release from the βislet cells of the pancreas.

Product name
Gliclazide
for Biochemistry

Wako CodePackage Size

071-04731

10g

Product nameTolbutamide
for Biochemistry

Wako CodePackage Size

209-09172

25g

Package Size

078-03881

5g

076-03882

25g

CAS No. 10238-21-8
C23H28ClN3O5S=494.00[Specification Assay] 98.0+% (Titration)[Solubility] N,N-Dimethylformamide[Overview] Sulfonylurea (SU) compounds
act by enhancing insulin release from the βislet cells of the pancreas.

Product name
Glimepiride
for Pharmacology Research

CAS No. 21187-98-4
C15H21N3O3S=323.41[Specification Assay] 98.0+% (Titration)[Solubility] N,N-Dimethylformamide[Overview] The pharmacological actions of
this product known as sulfonylurea (SU)include the action on the islands ofLangerhans. 1) Insulin secretion stimulation,2) somatostatin secretion stimulation, and3) glucagon secretion inhibition, etc. havebeen reported.

Wako Code

Wako CodePackage Size

071-05691

500mg

077-05693

5g

CAS No. 93479-97-1
C24H34N4O5S=490.62[Specification Assay] 97.0+% (HPLC)[Solubility] DMSO[Overview] Sulfonylurea (SU) compounds
act by enhancing insulin release from the βislet cells of the pancreas.

CAS No. 64-77-7
CH3C6H4SO2NHCONHCH2CH2CH2CH3=270.35[Specification Assay] 99.0+% (Titration)[Solubility] Ethanol[Overview] The pharmacological actions of
this product known as sulfonylurea (SU)include the action on the islands ofLangerhans. 1) Insulin secretion stimulation,2) somatostatin secretion stimulation, and3) glucagon secretion inhibition, etc. havebeen reported.

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