New Material Proposal

Green Papaya

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Kanehide Bio Co., Ltd

What is Papaya
・ Papaya is a representative of tropicalfruits meanwhile is eaten as vegetable inOkinawa.

・It contains much Vitamins as like vitaminsC, vitamins A and minerals as like calcium
or irons.

・ Green papaya contains much papain( proteolytic enzymes).
Picture：Papaya fruits

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Kanehide Bio Co., Ltd

How to use papaya①

Not only special market where local
materials gather in, we can see papaya
in general super market in the season.

Stewed papaya

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Kanehide Bio Co., Ltd

How to use papaya②

We can see shredded papaya as generalvegetables in super market.

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Fried papaya

Kanehide Bio Co., LtdBenefits of green papaya
1.Rich in papain enzymes
Papain enzymes known as pronominal of papaya, confirmed that green papayas
contain much papain enzymes however ripe papayas don't contain it much.
2.Rich in minerals and vitamins
Papaya contains much vitamins C compared with other fruits. Green papaya isrich in minerals as like iron, potassium, calcium and phosphorus more than ripe
papayas. Moreover we can say green papaya is a vegetable have much nutrientsas like β－carotene, citric acids, malic acids and dietary fibers.
3.High antioxidative activity
Green papaya has much polyphenol as well, vitamins C and these polyphenol
are base of green papaya’s antioxidant power.

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Kanehide Bio Co., Ltd

Components of green papaya (minerals)
215

0.35

0.3

210

potassium contents（ｍｇ/100g）

iron contents（ｍｇ/100g)

0.3
0.25

0.2
0.2
0.15

0.1
0.0504036calcium contents （ｍｇ/100g）

35302520201510205200
195190190185180

green papaya

ripe papaya
18

phosphorus contents （ｍｇ/100g）

ripe papaya

210

green papaya
171614121110864205

ripe papaya

green papaya

0

ripe papaya

green papayaGraph) minerals contents in green papaya and ripe papaya
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Kanehide Bio Co., LtdProliferation of bifidobacterium with green
papayaCulturemediumGeneral culture
Culture added
papaya extract
powderBacteria count
6.8×107／ｍL
5.4×108／ｍLFigure) viable bacterial count after 24 cultured

bacterial count(10 million／ｍL）

As result of the measuring viable bacterial count, as below figure show that theculture medium added papaya extract powder promote proliferating
Bifidobacterium. This measurement compared between general culture medium
and culture medium added papaya extract powder after 24 hours cultivation.
6050403020100

general culture

culture added papaya extract
powderGraph．Comparison of viable bifidobacterium
count after 24 hours cultured
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Kanehide Bio Co., LtdConfirmation ACE inhibition activity ofgreen papaya
As result of the test to see ACE inhibition activity which is one of indication
for blood pressure elevation inhibitory, we confirm that both of papaya ( ripeand immature ) have high ACE inhibition activity. Further compared withother vegetables and fruits from Okinawa, papaya has much higher activity.
Kinds of activity(confirm items)

PartACE inhibition
Immature
activity(blood pressure
elevation inhibitory
Ripeactivity)

Activityvalue(inhibitionrate %）
93.3
72.2

Reported by Okinawa industrial technology centerAll rights reserved.

Kanehide Bio Co., LtdHypotension activity of green papaya

Compared between a control group
and an mixing papaya group, after 8weeks, shown low value withmixing papaya group and after 12weeks, and 13 weeks shown
significantly lower.
As these results, we confirmed thatgreen papayas work improving
systolic blood pressure which is an
initial symptom of hypertension.

Control group

Systolic blood pressure

we measured value change ofmaximum blood pressure(systolicblood pressure) in SHR rat whichhave been taking feeds contained
10% of green papaya powder.

Mixing papaya group

Testing terms (weeks)
Figure) influence of feed mixed papaya against systolicblood pressure of SHR （*p<0.05）
Reported by Okinawa industrial technology centerAll rights reserved.

Kanehide Bio Co., LtdLipid metabolism improving activity of green
papayaAs the result, in mixed papaya group, shown
T-cho significant decreasing compared withcontrol group and TG decreased.
As these things, we confirmed immature
papaya help lowering concentration ofplasma cholesterol. Moreover we can expect
lipid metabolism improving activity.

Control group
Mixing papaya group

Concentration

we measured neutral fat （ TG) and total
plasma cholesterol （T-cho） of SHR rats
which have been taking feed mixed 10% ofgreen papaya powder. we measured neutralfat（TG) and total plasma cholesterol （Tcho） of SHR rats which have been taking
feed mixed 10% of green papaya powder.

Figure) influence of feed mixed papaya against plasmacholesterol and neutral fat of SHR（*p<0.05）
Reported by Okinawa industrial technology centerAll rights reserved.

Kanehide Bio Co., Ltd