The Next StageThe new Mastercycler® X50

Eppendorf Mastercycler X50 3»We cannot solve our problems
with the same thinking we used
when we created them.«
Albert Einstein, Physicist

The new Mastercycler X50 is the elegant synthesis of flexibility for research
applications and standardization for routine applications such as food testing etc.
A new highly intuitive touch screen concept puts all of those benefits always at
your fingertips.

OptimizationEppendorf once again pioneers
in the field of PCR optimization.
Our innovative 2D-Gradient allows
you to optimize two temperatures(e.g. denaturation and annealing)in one run while using Eppendorf’s
trusted SteadySlope® technology.

Speed
Take your PCR to the next level.
10 °C/s heating on an open blockformat gives you unexpected
speed. Your run times will neverbe the same. How fast do you
dare to go?

Standardization
Temperature verification tointernational standards, block
temperatures you can trust fromwell to well and run to run. These
are among the many benefits
we can offer, allowing your PCR
assays to contribute to a trusted
or even validated workflow.

> More information: page 6–7> More information: page 8–9> More information: page 9

4 Eppendorf Mastercycler X50

Eppendorf Mastercycler X50 5

The Next Stage of Engineering
Speed and enhanced optimization functions like the 2D-Gradientmake the Mastercycler X50 the ideal tool for advanced researchin molecular biology. The excellent block temperature control
and regulation give rise to the next stage of optimization, whereas
the adaptable user management and profound documentation
capabilities give peace of mind to laboratories working or
conforming to set standards.
A highly intuitive touch display, low noise levels, low power
consumption, and the versatile flexlid lid concept complete theproduct to be a powerful yet discrete tool. Up to 10 units can be
combined – ideal for high throughput applications or labs witha high number of users running different assays. Should you feelyou need more flexibility or throughput, up to 50 units can be
combined in a computer-controlled network.

Product Features:> Innovative 2D-Gradient for advanced PCR optimization> Heating rate: up to 10 °C/s
> Wide selection of blocks from a fast silver block to 384> Intuitive touch display> Connect up to 10 units to a network> flexlid® concept: automatic height adjustment ofthe lid allows you to use all types of consumables> Small footprint> 2 year warranty

Applications:> Fast PCR> PCR optimization> Standard PCR> Incubation> Cycle sequencing

Network like a pro
Control up to 10 cyclers withouta PC or additional hardware andup to 50 cyclers using a PC.

2D-Gradient
Two gradients in the samerun allow you to optimize theannealing and the denaturation
temperature in parallel. See ourApplication Note 387 for details.

Touch screen interfaceIntuitive and quickprogramming fromthe beginning

Fast ramping
With block heating rates of up to10 °C/s, you will have unrivalled
speed at your disposal. Use it
for the advancement of science.
See our Application Note 274
for details.

Quiet like a whisper
The Mastercycler X50 isdesigned to be impressively
quiet – even during extensive
cooling steps.

Dynamic homogeneity
Individual control of all six
peltier elements allows
dynamic homogeneity
regulation in real-time.

Small footprint
Ventilation from front to backcontributes to the small footprint of the Mastercycler X50.
No extra space is needed forventilation on either side ofthe cycler.

> Further information available at: www.eppendorf.com /mastercycler

6 Eppendorf Mastercycler X50

Eppendorf Mastercycler X50 7

The Next Stage of PCR Optimization
Optimizing the annealing temperature to improve PCR results isa routine task, so why not optimize as far as you can? More and more
findings indicate that optimization of the denaturation temperature
is worthwhile as well. A high denaturation temperature will harm theenzyme and other biomolecules in your assay. A low denaturationtemperature may result in inefficient splitting of the complimentary
DNA strands, as is often found with GC-rich templates or templates
prone to form »hairpin structures«.
Eppendorf’s new 2D-Gradient allows optimization of the annealing andthe denaturation temperature in a single run – taking less time thanever before. This gives you the ideal set of temperatures for your PCR
assay – quickly, conveniently, reliably.

99

98.5

97.3

95.6

93.4

72.3

71.7

70.5

68.5

65.9

63.2

60.8

58.2

55.4

53.5

TA

52.3

TD

51.9

Cross-Examination
PCR optimization of β-actin Gene with
2D-Gradient technique.
2D-Gradient allows you to optimize both thedenatura­tion temperature (bottom to top) and theannealing temperature (left to right) during thesame run. Higher denaturation temperatures canhave the advantage of increased specificity while
lower denaturation temperatures reduce stress on
biomolecules and can lead to increased yield. Assaysthat struggle to work reliably at a 95 °C denaturation
temperature could benefit significantly from theoptimization of the denaturation temperature.

91.7

90.6

90.2

> For technical details, please see our Application Note 387.

Eppendorf Mastercycler X50 98 Eppendorf Mastercycler X50

The Next Stage of Speed
PCR should be faster. That gives you quicker results,throughout the day, and a faster transition to optimal
temperatures. The Mastercycler X50 heats withmax. 10 °C/s and cools with max. 5 °C/s. Make the speedof the Mastercycler X50 your research advantage.

Thermal cycler

Total run time
[hh:mm:ss]

Mastercycler X50s
Mastercycler X50l
PeqSTAR 96X
Biorad® C1000
Agilent SureCycler® 8800
Proflex® (96-well)*
Mastercycler nexus gradientApplied Biosystems® Veriti Fast
SimpliAmp®*
Biorad T100*

00:39:29
00:45:02
00:47:10
00:49:18
00:50:33
00:50:54
00:51:15
00:56:13
00:56:44
01:03:52

Ramp rate according
to technical data[°C/s]
10555663544Make your PCR a standardized routine
The better you can control the reproducibility of yourPCR, the easier it is to run it consistently and to document the reproducibility of your workflow. Excellent block
homoge­neity, accuracy and precision as well as regulartemperature verification, stringent user management andadvanced connectivity are cornerstones of a validated PCR
workflow. The Mastercycler X50 supports your needs forinstrument qualification and method validation with thefollowing features:> Excellent block homogeneity (±0.2 °C at 20–72 °C)> Excellent block accuracy and precision (±0.15 °C)> Fast temperature verification possible> Adjustable verification settings – according to
your audit needs> Advanced documentation capabilities> Adjustable user management – from flexible to strict.
> Transparent performance data

There ia a large variety of cyclers available
with different ramp rates published.
If you compare the corresponding run times
by performing the same protocol using
different cyclers, you see that ramp rates
do not tell the full story about whether acycler is fast or not.

* Performed in high profile twin.tec plates because the cyclers cannot accommodate low profile plates.

Temperature verification with a multi-probe
system allows fast and reliable assessment of thecycler’s performance. The generated certificates
can support instrument qualification for yourquality management system.

> For technical details, please see our Application Note 274:
»Comparative run time evaluations of PCR thermal cyclers«.

10 Eppendorf Mastercycler X50

Eppendorf Mastercycler X50 11

Technical Specifications
DescriptionMastercycler® X50s

Mastercycler® X50a

Mastercycler® X50h

Mastercycler® X50i

Mastercycler® X50l

Mastercycler® X50t

Thermoblock

SilverAluminum

Aluminum

SilverAluminum

Aluminum

■

■

■

■

■

■

■

■

High pressure lid
96-well plate■384-well plate0.1/0.2 mL tubes

■

■

■

Temperature
control rangeof the block

4–99 °C

4–99°C

Temperature
control mode

Fast, Intermediate, Standard, SafeFast, Intermediate, Standard, Safe

Six peltier elements

Six peltier elements

Heating technologyof the block
2D-Gradient block

over 12 columns /over 8 rows

over 24 columns /over 16 rows

over 12 columns /over 8 rows

over 24 columns /over 16 rows

Gradient range

1–30 °C

1–30 °C

Gradient
temperature range

30–99 °C

30–99 °C

Lid temperaturerange

37–110 °C

37–110 °C

Lid descentflexlid

flexlid

Block homogeneity:
20 °C–72 °C
95 °C≤ ±0.2 °C≤ ±0.3 °C≤ ±0.2 °C≤ ±0.3 °C± 0.15 °C± 0.15 °C

Block temperatureaccuracy
Heating rate

max. 10 °C/s

max. 5 °C/s

max. 10 °C/s

max. 5 °C/s

Cooling rate

max. 5 °C/s

max. 2.3 °C/s

max. 5 °C/s

max. 2.3 °C/s

Interfaces

Ethernet, USB

Ethernet, USB

Dimensions(W × D × H)

27.5 × 43 × 33 cm

27.5 × 43 × 33 cm

Weight11.5 kg

10.7 kgPower supply

110–230 V, 50–60 Hz

110–230 V, 50–60 Hz

Max. power
consumption

850 W

850 WOrdering information
Mastercycler® X50s, silver block, 96-well plate or 0.1/0.2 mL tubes
Mastercycler® X50a, aluminum block, 96-well plate or 0.1/0.2 mL tubes
Mastercycler® X50h, aluminum block, 384-well plate, high pressure lid
Mastercycler® X50i*, silver block, 96-well plate or 0.1/0.2 mL tubes
Mastercycler® X50l*, aluminum block, 96-well plate or 0.1/0.2 mL tubes
Mastercycler® X50t*, aluminum block, 384-well plate, high pressure lid

International Order no.
6311 000 010
6313 000 018
6316 000 019
6301 000 012
6303 000 010
6306 000 010

North America Order no.
6311000010
6313000018
6316000019
6301000012
6303000010
6306000010Accessories
Ethernet cable, 5 m

6313 070 040

6313070040* to operate this unit, it needs to be connected to a Mastercycler X50 s,a,p, or h. Up to 9 units can be connected to a Mastercycler X50 s,a,p, or h.

Sign up for free!
Real-Time MonitoringMonitor your PCR run from anywhere and track
the parameters, e.g. runtime or device status

Alarm Management
Track all alarm acknowledgements with
time-stamped data logs

Instant Notifications
Receive alarm or event notifications,e.g. finished program or error messages
to react quickly

Response-Based Alarm EscalationD
 efine escalation scheme to individual
or multiple recipient groups based onlab presence

Device Connectivity
Connect and monitor all your Eppendorf
PCR cyclers*Task Management
 lan and assign device-related tasks, e.g. clean
P
block or schedule maintenance service. Tasks
can be tracked retrospectively via Task History

Easy Data AccessExport parameter, event data and any
user interactions* The VisioNize box is required for Eppendorf Mastercycler nexus models.
** Additional device require extra device licences. Hardware and software upgrades are not included.

Connect up to three cyclers for free**!
Contact us for more information: www.eppendorf.com/visionize

Your local distributor: www.eppendorf.com/contact
Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com

www.eppendorf.com/mastercycler
BIO-RAD® C1000 is a registered trademark of Bio-Rad® Laboratories, Inc., USA. Agilent SureCycler® 8800 is a registered trademark of Agilent Technologies, Inc., USA.
Proflex® is a registered trademark of Life Technologies Corp., USA. Applied Biosystems® Veriti Fast is registered trademark of Applied Biosystems LLC, USA. SimpliAmp® is a registered trademark of Life Technologies Corp., USA.
Eppendorf®, the Eppendorf Brand Design, Mastercycler®, SteadySlope® and flexlid® are trademarks of Eppendorf SE, Germany. All rights reserved, including images and graphics.
Order No.: A631112020/EN4/1123/MCP Copyright © 2023 by Eppendorf SE.