Environmental
Environmental
Analytical
Chemistry
Analytical Chemistry1.

Dioxins Analysis

1-1. Presep® Series for Dioxins Clean up .................................................................. 3
Presep® Multilayer Silica Gel ......................................................................................4
Presep® Active Carbon-impregnated Silica Gel...........................................................5
Presep® Acitive Carbon-impregnated Silica Gel (Reverse Column) .............................5
Related products........................................................................................................6

1-2. Standard................................................................................................................... 6
2,2’,3-Trihydroxybiphenyl Standard.............................................................................6

1-3. Solvents for Dioxins Analysis............................................................................... 6

2.
3.

4.

PFCs Analysis

Presep®-C PFC(short), Presep®-C PFC-II................................................................. 8

Water Quality Testing

3-1. Anionic Surfactant Analysis (Linear Alkylbenzene Sulfonate : LAS).......... 10
3-2. Standards............................................................................................................... 13
3-3. Musty-Odor Standard Analysis......................................................................... 14

Food Analysis

4-1. Melamine Standards............................................................................................ 15
4-2. A Clean up of Aflatoxins...................................................................................... 16
AFLAKING; Immunoaffinity Colums........................................................................... 16

5.

Biocide6.

Metal Standard Solutions

7.

8.
9.

Dimethylfumarate (DMF) Standard............................................................................ 17

JCSS ....................................................................................................................... 18

Solvents

7-1. for Pesticide Residue and PCB Analysis.......................................................... 19
7-2. for LC/MS................................................................................................................ 20
7-3. for HPLC................................................................................................................. 20

Pretreatment Columns

Presep® Series............................................................................................................... 23

High-sealed Storage Bottles

High-sealed Storage Bottles...................................................................................... 24

Alphabetical Index
page
21
20
20
19
19
6
20
20
9
19
19
19
7
16
16
15
15
21
21
21
14
12, 13
20
19
19
20
19
19
20
20
19
19
20
7
19
19
21
6
21
7
19
19
19
21
14
7
19
19
7
17
7
21
21
7
21
19
21
19
19
7
19
19
21
7
20
21
20
14
14
9
9
9
9
9
21
21
7
19
19
21
24
15
15
18
7
20
9
19

A

B

C

D

E

F
G
H

I
M

Description

Acetic Acid (for HPLC)
Acetic Acid (for LC/MS)
Acetone (for HPLC)
Acetone 300
Acetone 5,000
Acetone (for Dioxin)
Acetonitrile (for HPLC)
Acetonitrile (for LC/MS)
Acetonitrile (for PFOS · PFOA)
Acetonitrile (for Tiuram)
Acetonitrile 300
Acetonitrile 5,000
Active Carbon-impregnated Silica Gel
AFLAKING 25
AFLAKING 50
Ammelide Standard
Ammeline Standard
1 mol / L Ammonium Acetate Solution (for HPLC)
1 mol / L Ammonium Dihydrogenphosphate Solution (for HPLC)
1 mol / L Ammonium Formate Solution (for HPLC)
AnilineAnionic Surfactants Mixture Standard Solution
Benzene (for HPLC)
Benzene 300
Benzene 5,000
1-Butanol (for HPLC)
t-Butyl Methyl Ether 300
t-Butyl Methyl Ether 5,000
t-Butyl Methyl Ether (for HPLC)
Chloroform (for HPLC)
Chloroform 300
Chloroform 5,000
Chloroform, Amylene added (for HPLC)
Copper, Reduced, Granular, 300-850µm(20-50mesh)
Cyclohexane 300
Cyclohexane 5,000
Cyclohexane (for HPLC)
Decane (for Dioxin)
o-Dichlorobenzene (for HPLC)
Dichloromethane (for Dioxin)
Dichloromethane 300
Dichloromethane 5,000
Dichloromethane 5,000, 2-Methyl-2-butene added
Dichloromethane (for HPLC)
2,4-Dichlorophenol Standard
Diethyl Ether (for Dioxin)
Diethyl Ether 300
Diethyl Ether 5000
Diethylene Glycol (for Dioxin)
Dimethyl Fumarate Standard
Dimethyl Sulfoxide (for Dioxin)
N,N-Dimethylformamide(for HPLC)
1,4-Dioxane(for HPLC)DIOXIN TRAP BEADSDistilled Water(for HPLC)Distilled Water, Hexane WashedEthanol (99.5) (for HPLC)
Ethanol 300
Ethanol 5,000
Ethanol(99.5) (for Dioxin)
Ethyl Acetate 300
Ethyl Acetate 5,000
Ethyl Acetate (for HPLC)
Fluorobenzene (for Dioxin)0.1vol% Formic Acid-Acetonitrile (for LC/MS)
Formic Acid (abt. 99%) (for HPLC)
Formic Acid (abt. 99%) (for LC/MS)
Geosmin Standard
Geosmin Standard Solution
1H,1H,2H,2H-Henicosafluoro-1-dodecanol Standard
Henicosafluoroundecanoic Acid Standard
Heptacosafluorotetradecanoic Acid Standard
Heptadecafluorononanoic Acid Standard
Heptafluorobutanoic Acid Standard
Heptane (for HPLC)
1,1,1,3,3,3-Hexafluoro-2-propanol (for HPLC)
Hexane (for Dioxin)
Hexane 300
Hexane 5,000
Hexane (for HPLC)
High-sealed Storage Bottle, Brown
Isocyanuric Acid Standard
Melamine Standard
Metal Standard Solutions (JSCC)
Methanol (for Dioxin)
Methanol (for LC/MS)
Methanol (for PFOS · PFOA)
Methanol 300

page
19
21
21
14
14
14
9
9
7
13
13
14
9
7
19
19
21
7
21
9
23
23
5
5, 23

M

N

P

5, 23
12, 23
23
23
23
23
23
23
23
4, 23
9, 23
23
23
23
23
23
23
12, 23
23
12, 2312, 23
23
23
23
23
23
23
23
9, 23
23
23
23
21
20
21
7
21
12
12
12, 1312, 13
12
12
12
21
21
7
19
19
21
14
9
9
21
21
6
21
20
9
9
16
12, 1312, 13
9
12

S

T

U
W

Description

Methanol 5,000
Methanol (for HPLC)
1-Methyl-2-pyrrolidone (for HPLC)
2-Methylisoborneol-Geosmin Mixture Standard Solution
2-Methylisoborneol Standard
2-Methylisoborneol Standard Solution
Nonadecafluorodecanoic Acid Standard
Nonafluoropentanoic Acid Standard
Nonane (for Dioxin)
Nonylphenol (mixture of isomers)
p-n-Nonylphenol Standardp-(1,1,3,3-Tetramethylbutyl) phenol Standard
Pentadecafluorooctanoic Acid Standard
Petroleum Ether (for Dioxin)
Petroleum Ether 300
Petroleum Ether 5,000
Phosphoric Acid (for HPLC)
Phthalocyanine Immobilized Silica Gel
0.25mol/L Potassium Dihydrogenphosphate Solution (for HPLC)
Potassium Nonafluorobutanesulfonate Standard
Presep® (Luer Lock) Diatomaceous Earth, Granular Type M
Presep® (Luer Lock) Polyamide C-200 Type M
Presep® Active Carbon-blended Silica Gel
Presep® Active Carbon-impregnated Silica Gel
Presep® Active Carbon-impregnated Silica Gel(Reverse
Column)Presep® Agri
Presep® C18(ODS)
Presep® C18(ODS) Type M
Presep® CM
Presep® DEA
Presep® Dehydration, 48WELL PLATE
Presep® Diatomaceous Earth, Granular
Presep® Florisil®
Presep® Multilayer Silica Gel
Presep® PFC-II
Presep® PolyChelate
Presep® QA
Presep® RPP
Presep® RPP-SAX
Presep® RPP-WAX
Presep® S
Presep®-C Agri (Short)
Presep®-C Alumina
Presep®-C C18 (ODS)
Presep®-C C18 (ODS) (Short)
Presep®-C DNPH
Presep®-C DNPH(Short)
Presep®-C Florisil®
Presep®-C Na2SO4
Presep®-C NH2
Presep®-C NH2 (Short)
Presep®-C Ozone Scrubber
Presep®-C PFC (Short)
Presep®-C RPP(Long)
Presep®-C RPP(Short)
Presep®-C Silica Gel
1-Propanol (for HPLC)
2-Propanol (for LC/MS)
2-Propanol (for HPLC)
10% Sodium Chloride Solution (for Dioxin)0.25 mol/L Sodium Dihydrogenphosphate Solution (for HPLC)
Sodium Decylbenzenesulfonate Standard Solution
Sodium Dodecylbenzenesulfonate Standard Solution
Sodium p-n-Octylbenzenesulfonate Standard
Sodium p-n-Octylbenzenesulfonate Standard Solution
Sodium Tetradecylbenzenesulfonate Standard Solution
Sodium Tridecylbenzenesulfonate Standard Solution
Sodium Undecylbenzenesulfonate Standard Solution
Tetrahydrofuran, Stabilizer Free (for HPLC)
Tetrahydrofuran, with Stabilizer (for HPLC)
Toluene (for Dioxin)
Toluene 300
Toluene 5,000
Toluene (for HPLC)
2,4,6-Trichloroanisole Standard
Tricosafluorododecanoic Acid Standard
Tridecafluoroheptanoic Acid Standard
Trifluoroacetic Acid
0.1vol% Trifluoroacetic Acid - Acetonitrile (for HPLC)
2,2',3-Trihydroxybiphenyl Standard
2,2,4-Trimethylpentane (for HPLC)
Ultrapure Water (for LC/MS)
Ultrapure Water (for PFOS · PFOA)
Undecafluorohexanoic Acid Standard
Wakopak® Wakosil 5NH2
Wakopak® Navi C18-5
Wakopak® Wakosil AS-Aqua
Wakopak® Wakosil-II3C18RS
Wakosil® AS-Aqua Eluent

F

Dioxins Analysis

1-1.

Dioxins Analysis

11.

Presep® Series for Dioxins Clean up

or analysis of dioxins, samples are treated for cleanup by multilayer silica
gel column chromatography or activated carbon silica gel
chromatography. These cartridge columns are filled with solid-phase carriers
for the treatment.

Features

Design conforming to JIS K 0311 (Method for determination of dioxins and coplanar PCB in exhaust gas) and JIS K 0312
(Method for determination of dioxins and coplanar PCB in industrial water and waste water)
1. The column plug and moisture-proof aluminum packaging bag are used to prevent quality deterioration during storage
to keep stable quality.
2. Suitability for analysis of dioxins * (Blank test for dioxins and coplanar PCB by high-resolution GC/MS has been
implemented.)
3. The use of eluent is reduced by the use of reverse elution method. (Presep® Active Carbon-impregnated Silica Gel
Reverse Column)* Except Presep® Multilayer Silica Gel

Dioxins analysis flow
Analytical sample
Soxhlet extraction
Concentration
Sulfuric acid treatment
Silica gel chromatography

Wakogel® DX(Wako cat. No. 238-01781)
see the page #4

Multilayer silica gel column
chromatography

Presep® Multilayer Silica Gel(Wako cat. No. 295-41651)
see the page #4

Concentration
Alumina column chromatographActivated carbon silica gel chromatography

Presep® Activated Carbon-impregnated Silica Gel(Wako cat. No. 293-41451)

see the page #5(Wako cat. No. 299-41551)

see the page #5(Wako cat. No. 297-43051)

see the page #5

Presep® Activated Carbon-blended Silica Gel
Presep® Activated Carbon-impregnated Silica Gel(Reverse Column)
Concentration/solvent substitution
GC/MS

Environmental Analytical Chemistry3Presep® Multilayer Silica Gel

1

A

Dioxins Analysiss one of the pretreatment processes of dioxins analysis, clean up using a
multilayer silica gel column with various kinds of chemically modified silica gellaminated is carried out in order to efficiently remove foreign substances such ascompounds containing sulfur, polycyclic aromatic hydrocarbons, coloring substances,
etc. which coexist in measured specimens. However, the packing operation of
preparing the multilayer silica gel column to be used for this analysis is extremely
troublesome. Wako has launched a product with various kinds of chemically modified
silica gel laminated in a glass column.

Column configuration

Column chromatography tube: Glass tube with inside diameter of 15 mm
Presep® Column Adapter (PTFE)

silica gel, 0.9g

silica gel, 0.9g
silica gel, 0.9g

Glass fiber filter
Sodium sulfate: 6g
10% silver
nitrate-impregnated
22% sulfuric acidsilica gel, 6g
44% sulfuric acid silica gel, 4.5g
2% potassium hydroxide silica gel, 3g
Glass fiber filter

Presep® Cock Adapter

Presep® Multilayer Silica Gel

Product Name

Package Size

Wako Cat. No.

5 units

295-41651

Example of clean up of soil extracted sample using Presep® Multilayer Silica Gel after treatment.
[Before treatment]
2[After treatment]

5

3

1

1. 2,4,6-Trichlorophenol
2. Propyzamide
3. Anthracene
4. N,N-Bis (1-methylethyl)-benzamide
5. Lenacil
6. Bis(2ethylhexyl)phthalate
7. Terpenes

6
4

7<Related Products>
Package Size

Wako Cat. No.

2% Potassium Hydroxide-impregnated Silica Gel

Product Name

100 g

167-19251

10% Silver Nitrate-impregnated Silica Gel

100 g

197-11611

22% Sulfuric Acid-impregnated Silica Gel

100 g

194-11621

100 g

191-11631

55% Sulfuric Acid-impregnated Silica Gel

100 g

197-13811

Sodium Sulfate

250 g

194-12221

Wakogel® DX

100 g

238-01781

44% Sulfuric Acid-impregnated Silica Gel

4

Environmental Analytical Chemistry

Gradefor Dioxins Analysis

Glass column tube

Example of use

Dioxins Analysis

Column configuration

1

Presep® Active Carbon-impregnated Silica Gel
Presep® Active Carbon-blended Silica Gel
Glass reservoir

Cotton ballLuer Stop Valve

Activated carbon-impregnated silica gelor activated carbon-blended silica gel
Glass ﬁber ﬁlter

or

Cotton ball

Product Name

Presep® Active Carbon-impregnated Silica Gel
Presep Active Carbon-blended Silica Gel®

Package Size

Wako Cat. No.

10 each293-41451

10 each299-41551

Presep® Active Carbon-impregnated Silica Gel (Reverse Column)
Column configuration

Example of use (Connection of drip funnel)
Drip funnel

PTFE seal
Columnar absorbent cotton
Activated carbon-impregnatedsilica gel, 1 g

Reducer (PTFE)

Columnar absorbent cotton

Carbon-impregnated Silica Gel(Reverse Column)

PTFE seal

Joint (TS 15 / 25)

Product Name

Presep® Acitive Carbon-impregnated Silica Gel (Reverse Column)

Package Size

Wako Cat. No.

5 each

297-43051

Examples of reagent blank of dioxins of active carbon silica gel
Dioxins
T4CDDsP5CDDsH6CDDsH7CDDsO8CDD
T4CDFsP5CDFsH6CDFsH7CDFs
OCDF

Active Carbon-impregnatedSilica Gel
0.2 ↓
0.2 ↓
0.2 ↓
0.5 ↓
2 ↓
0.2 ↓
0.2 ↓
0.2 ↓
0.5 ↓
2 ↓

Active Carbon-blended
Silica Gel
0.2 ↓
0.2 ↓
0.2 ↓
0.5 ↓
2 ↓
0.2 ↓
0.2 ↓
0.2 ↓
0.5 ↓
2 ↓

Dioxins
3,4,4',5-T4CB（#81）
3,3',4,4'-T4CB（#77）
2,3',4,4',5-P5CB（#118）
2,3,4,4',5-P5CB（#114）
2,3,3',4,4'-P5CB（#105）
3,3',4,4',5-P5CB（#126）
2',3,4,4',5-P5CB（#123）
2,3',4,4',5,5'-H6CB（#167）
2,3,3',4,4',5-H6CB（#156）
2,3,3',4,4',5'-H6CB（#157）
3,3',4,4',5,5'-H6CB（#169）

Active Carbon-impregnatedSilica Gel
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓

（unit : pg/g）

Active Carbon-blended
Silica Gel
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓
1 ↓

Fractionation Performance Test
[Presep® Active Carbon-impregnated Silica Gel]

[Presep® Active Carbon-blended Silica Gel]

Environmental Analytical Chemistry5

1<Related Products>

Dioxins Analysis

Product Name

Use

Presep® Column Adapter (TS15/25)

Package Size

Presep® Column Adapter (TS19/38)

Part for connecting Presep® Multilayer Silica Gel andseparating funnel with interchangeable ground joint

Presep® Column Adapter Packing

Refill Teflon packing (white) for Presep® Column Adapter(1) Joint to be fitted on the sample elution side of Presep
Active Carbon-impregnated Silica Gel (Reverse Column)(2) These joints are usable also as adapters on the eluent
injection side by fitting a separating funnel withinterchangeable ground joint.
®

Presep® Column Joint (TS15/25)
Presep® Column Joint (TS19/38)

Wako Cat. No.

1 each295-42251

1 each299-45951

5 each298-50051

1 each291-42851

1 each

297-42951

Presep® Column Reducer (PTFE)

Connection of Presep® Active Carbon-impregnated Silica
Gel (Reverse Column) and adapter on eluent injection side

1 each295-42751

Presep® Cock Adapter

Adapter with cock to be fitted on sample elution side (underchromatographic column) of Presep® Multilayer Silica Gel.

1 each299-42151

Presep® Cock Adapter Packing

Refill Teflon packing (brown) for Presep® Cock Adapter

5 each294-50151

Presep® Cylinder Adapter (PTEE)

Connection of Luer Stop Valve and reservoir (solvent
reservoir) in the case of use of Presep® Alumina, ActivatedDX, Presep® Active Carbonimpregnated Silica Gel or
Presep® Active Carbon-blended Silica Gel

5 each20 each291-41751
297-41753

1-2.

Standard

2,2’,3-TrihydroxybiphenylEnvironmental pollution caused by dioxins has spread worldwide and has become a serious social issue.
Environmental dioxins can be degraded by heat or chemical treatments, but there is a demerit because the treatment
of dioxins in soil or river sludge is very expensive.
Recently, degradation using microorganisms has been investigated as one of the dioxin treatments and the search formicroorganisms using dioxins as a carbon source is being carried out.
This product is used for substrates of dioxin-degrading microorganisms.

Reaction pathway
OH

O
Dibenzofuran

O

OH

O

OH

HO

OH
OH

OH

2,2',3-Trihydroxybiphenyl

HO

O

OH
OH

OH

OH
O

Product Name

2,2’,3-Trihydroxybiphenyl Standard

Package Size

Wako Cat. No.

100 mg208-15551

1-3. Solvents for Dioxins Analysis

These products are used to determine Dibenzo-p-dioxins, Dibenzofurans, and Coplanar PCBs by high resolution
GC/MS, and ensures a sufficiently low concentration.
Product Name

Acetone, 99.8+% (cGC)

Wako Cat.
No.
010-17831
016-17833

Specifications
Suitability for dioxins determination

PackageSize
1L
3L

Density (20℃): 0.789~0.792 g/mL

Dibenzo-p-dioxin(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Coplanar PCB

Decane, 99.5+% (cGC)

048-28543
042-28541

2 mL × 5
100 mL

Density (20℃): 0.727~0.735 g/mL

Dibenzo-p-dioxin

: ≤10
: ≤50

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤10
: ≤50

Environmental Analytical Chemistryfor other products, please visit the Wako Online Catalog

-

pg/L

: ≤10

(Cl: 4 ~ 6)
(Cl: 7 or 8)

Coplanar PCB

6-(unit)

fg/μL

: ≤10

https://labchem-wako.fujifilm.com/

[Methylene Chloride]99.5+% (cGC, except MeOH)
Stabilizer: Methanol [0.2~0.5%]

Diethylene Glycol, 99.0+% (cGC)

Diethyl Ether, 99.5+% (cGC)
Ethanol (99.5), 99.5+% (cGC)[Ethyl Alcohol (99.5)]

Fluorobenzene, 98.0+% (cGC)

Specifications
Suitability for dioxins determination

PackageSize048-26321
044-26323

1L
3L

040-28645

500 mL049-27451

1L

050-06661
056-06663

1L
3L

061-04551

200 mL

Density (20℃): 1.320~1.331 g/mL

Solubility in water: to pass test
Density (20℃): 1.116~1.123 g/mL

Density (20℃): 0.712~0.714 g/mL

Density (20℃): 0.789~0.792 g/mL

Density (20℃): 1.025~1.035 g/mL

Dibenzo-p-dioxin(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Dibenzo-p-dioxin(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤5
: ≤25

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤5
: ≤25

Coplanar PCB(2,3',4,4',5-PeCB)(Other Co-PCB): ≤100: ≤50

Dibenzo-p-dioxin(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Coplanar PCB

-

: ≤10

Dibenzo-p-dioxin(Cl: 4 ~ 7)
(Cl: 8)

: ≤5
: ≤10

Dibenzofuran(Cl: 4 ~ 7)
(Cl: 8)

: ≤5
: ≤10

-

Coplanar PCB

Hexane, 96.0+% (cGC)[n-Hexane]

Methanol, 99.8+% (cGC)[Methyl Alcohol]

Nonane, 98.0+% (cGC)[n-Nonane]083-07391
089-07393

1L
3L

Density (20℃): 0.658~0.662 g/mL

136-13461
132-13463

1L
3L

Density (20℃): 0.791~0.793 g/mL

148-07351
142-07354

2 mL × 5
100 mL

Density (20℃): 0.713~0.721 g/mL

Dibenzo-p-dioxin

: ≤1
: ≤5

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

-

Petroleum Ether
Content: 5~15 %

160-20231

1L

Boiling range (30~60℃): 90+vol%(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤5
: ≤10

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤5
: ≤10

-

10% Sodium Chloride Solution

192-12641

1L-(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Dibenzofuran(Cl: 4 ~ 6)
(7, 8 chlorinated)

: ≤1
: ≤5

-

: ≤10

Dibenzo-p-dioxin(Cl: 4)(Cl: 5 or 6)(Cl: 7 or 8)

: ≤1
: ≤2
: ≤5

Dibenzofuran(Cl: 4)(Cl: 5 or 6)(Cl: 7 or 8)

: ≤1
: ≤2
: ≤5

-

Coplanar PCB

Toluene, 99.7+% (cGC)

203-14141
209-14143

1L
3L

Density (20℃): 0.864~0.868 g/mL

Dimethyl Sulfoxide, 99.0+% (cGC)

041-29395

500 mL

Density (20℃):1.100 ~ 1.106 g/mL

fg/μL

pg/L

fg/μL

pg/L

pg/L

: ≤5

Dibenzo-p-dioxin(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

Dibenzofuran(Cl: 4 ~ 6)
(Cl: 7 or 8)

: ≤1
: ≤5

-

: ≤10

Dibenzo-p-dioxin(Cl: 4 ~ 7)
(Cl: 8)

: ≤5
: ≤10

Dibenzofuran(Cl: 4 ~ 7)
(Cl: 8)

: ≤5
: ≤10

Coplanar PCB

-

: ≤5

Coplanar PCB

pg/L

: ≤5

Dibenzo-p-dioxin

Coplanar PCB

pg/L

: ≤10

Dibenzo-p-dioxin

Coplanar PCB

pg/L

: ≤5

(Cl: 4 ~ 6)
(Cl: 7 or 8)

Coplanar PCB(unit)

Dioxins Analysis

Dichloromethane

Wako Cat.
No.

1

Product Name

pg/L

fg/μL

<Related Products>
Package Size

Wako Cat. No.

DIOXIN TRAP BEADS

Product Name200 g

040-27481

Active Carbon-impregnated Silica Gel

10 g

019-11941

Copper, Reduced, Granular, 300-850 µm (20-50 mesh)

50 g

032-19571

Phthalocyanine Immobilized Silica Gel

5g
25 g

160-21831
168-21832

Environmental Analytical Chemistry

7

2

2.

PFCs Analysis

PFCs Analysis

Presep®-C PFC (short)
Presep®-C PFC-II

A

ccumulation of perfluorocarbons in the body is aconcern because of high lipid-solubility anddegradation difficulty. Various environmental laboratories
have examined analytical methods since it was listed as
an item to be investigated by the Ministry of theEnvironment in fiscal 2002, in Japan.

Presep®-C PFC (short) and Presep®-C PFC-II are solid-phase extraction columns filled witha divinyl benzene-polymethacrylate resin-based filler. Presep® PFC-II is filled with a newpolymer developed for the purpose of highly efficient recovery of wide range of PFCs. Thesecolumns are used as pretreatment columns for PFCs, such as perfluorooctanoic acid(PFOA) and perfluorooctanesulfonic acid (PFOS).

Features

1. Trace PFCs in water can be highly efficiently recovered. Presep® PFC-II can recover 16 PFCs.
2. 16 components can be analyzed simultaneously and quickly by combining with Wakopak® Wakosil-II 3C18 RS.
3. Blank caused by solvent can be reduced by combining with a solvent for PFOS or PFOA analysis.

PFCs additive recovery test
● PFCs reference standard is added to purified water, and the recovery is measured. Analyzed components: 16 components

Solid-phase extraction conditions
Column conditioning :Column : Presep ® PFC-Ⅱ
CH3OH with 0.1 % NH4OH 10 mL
5 mL
CH3OH
H 2O
5 mL ※1

Results of recovery of additives in purified water
SampleName

Column : Presep ® -C PFC
CH3OH 10 mL
5 mL ※1
H2O

Collection/concentration operation
Water sample (additive recovery experiment: Addition of standard to 1 L of puriﬁed water)↓
Feed of water into column (ﬂow velocity: 10 to 20 mL/min)↓Drying (air purging or nitrogen gas purging)↓
Elution: Amount of solvent: 2 mL (Elution solvent) Presep® PFC-Ⅱ : CH3OH with 0.1 % NH4OH 2 mL↓
Presep® -C PFC : CH3OH
2 mL
LC/MS/MS measurement

Ion exchange water fed into unused Presep® cartridge which has been exposed to recovery experiment is used.
Ultrapure water for PFOs/PFOA analysis (212-01363) can be used.
Contamination from air can be prevented by connecting unused Presep® in the previous stage of the column
which has collected the sample and sucking air.
The eluate is concentrated by nitrogen gas purging according to the measurement sensitivity, and the quantity ismeasured.

PFBS
PFHxS
PFOS
PFDS
PFBA
PFPeA
PFHxA
PFHpA
PFOA
PFNA
PFDA
PFUnDA
PFDoDA
PFTeDA
PFHexDA
PFODA

Presep®
Presep®
PFC-Ⅱ
C-PFC
Rec overy (%) Rec overy (%)※The components indicated by boldface
are assayed in product test.

Chromatograms of PFCs additive recovery test (Presep® -C PFC (short))
2.4e4
2.2e4
2.0e4

③

1.8e4
1.6e4
1.4e4

⑧

②

1.2e4

④

1.0e4

⑤

①

8000.0① PFOA⑤ PFOS② PFHxS⑥ PFUnDA③ PFNA⑦ PFDoDA④ PFDA⑧ PFDS⑥

6000.0⑦4000.02000.0
0.0

8

6

7

8

9

10

11

Environmental Analytical Chemistry

12

13
14
Time, min

15

16

17

18

19

20

21

for other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

LC/MS/MS measurement of PFCs
2
PFCs Analysis

MS/MS conditions

HPLC analysis conditions

Solid-Phase Extraction Columns
Product Name

Presep -C PFC (Short)®

Package Size

Presep® PFC-II

Wako Cat. No.

10 each × 5

297-49651

10 each × 10

291-33441<Related Products>
HPLC Column
Product Name

Wakopak Wakosil-II3C18RS®

Size

Package Size

Wako Cat. No.

2.0 mm × 150 mm (D)
2.0 mm × 150 mm (W)

1 unit1 unit236-50431
232-50433(D); DuPont type, (W); Waters type

Solvents
Product Name

Ultrapure Water

Gradefor PFOS · PFOA
Analysis

Acetonitrile
MethanolPackage Size

Wako Cat. No.

1L
3L

216-01361
212-01363

1L

011-22251

1L

130-15941

Standards
Package Size

Wako Cat. No.

Heptacosafluorotetradecanoic Acid Standard

Product Name

100 mg080-08641

1H,1H,2H,2H-Henicosafluoro-1-dodecanol Standard

100 mg080-08761

Heptafluorobutanoic Acid Standard

100 mg087-08771

Heptadecafluorononanoic Acid Standard

100 mg088-08681

Henicosafluoroundecanoic Acid Standard

100 mg089-08611

Nonadecafluorodecanoic Acid Standard

100 mg144-08551

Nonafluoropentanoic Acid Standard

Gradefor Environment
Analysis

100 mg145-08581

Pentadecafluorooctanoic Acid Standard

500 mg

164-21851

Potassium Nonafluorobutanesulfonate Standard

100 mg169-24341

Tricosafluorododecanoic Acid Standard

100 mg204-17091

Tridecafluoroheptanoic Acid Standard

100 mg209-17041

Undecafluorohexanoic Acid Standard

100 mg212-01341

Environmental Analytical Chemistry9

3
Water Quality Testing3.

Water Quality Testing

3-1.

Anionic Surfactant Analysis

Complies with the revised Water Works Law (in Japan)W

ith water quality criteria and test methods being revised, so did the anionic surfactant analysis method; it has changed from flow
injection absorptiometry to high performance liquid chromatography. HPLC-fluorescence detection is adopted in the revised law.
With this method, a column packed with silica gel, which is chemically modified with octadecylsilyl group (ODS column) or a column withequivalent quality to ODS column, is used as the separating column. According to this method, when analysis of water is carried out
using Wakopak® Navi C18-5 (ODS column), numerous peaks may be detected.
On the other hand, the amount of anionic surfactant is prescribed a the total amount in the water quality criteria. Therefore, if these peaks
are reduced, it improves the detectability and simplifies the quantitative calculation. Wakopak ® Wakosil AS-Aqua is packed with the filler
which recognizes the number of carbon atoms and not the branched condition, and is the best column for simple analysis of anionicsurfactants.
According to the change in the analysis method, anionic surfactants of C10-C14 including branched alkyl chain are available as areference standard. Presep®-C C18, solid-phase extraction column, is also available for pretreatment of samples. This column is hardly
affected by the quality of water samples and good recovery results are obtained. The combined use of these products is recommended.

C10H21

C11H23
C16H25NaO3S

C12H25
C17H27NaO3S

SO3Na

C18H29NaO3S

SO3Na

SO3Na

C13H27

C14H29
C19H31NaO3S

C20H33NaO3S

SO3Na

SO3Na

Sample Pretreatment
Conforming to revised Waterworks Law (enforced on Apr. 1st, 2004 in Japan)

Solid-phase extraction conditions↓
Conditioning：① CH3OH 5 mL
5 mL
↓ ② H 2O
Sample: Water sample※） 1 L 20 mL/min.
↓Drying：Suction of air for 5 min(N2 gas purging for 5 min)↓Elution：CH3OH 5 mL↓
(Concentration: To 2 mL by N2 gas blowing)
Omitted in this process↓
HPLC analysis※）1 L of purified water + 0.1 mg of each reference standard

1 L of tap water + 0.1 mg of each reference standard

10

Environmental Analytical Chemistry

RecoveryComparison of recovery of additive (purified water)

Comparison of recovery of additive (tap water)

110

110

100

100

90

90

80

80

70

70

Recovery (%)

Presep®-C C18（ODS）
Presep®-Agri
Polymer-based SPE(competitor’s product)

Recovery (%)

Column：

60
50
40
30
20

60
50
40
30
20

10

10

0

C10

C11

C12

C13

C14

Anionic surfactant

Presep -C C18
Presep -Agri
Polymer-based SPE (competitor's product)
R

R

0

C10

C11

C12

C13

C14

Anionic surfactant

Presep -C C18
Presep -Agri
Polymer-based SPE (competitor's product)
R

R

Presep -C C18 is hardly affected by the quality of water sample and gives excellent recovery results.
Rfor other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

Analysis of standard solution for anionic surfactant test HPLC analysis conditions
Analysis of standard solution for anionic surfactant test HPLC analysis conditions

Injection ：each 10 mg/L（CH3OH）、10 μL
Sample ：Standard solution for anionic surfactant test
(sodium alkylbenzene sulfonate: C10, C11, C12, C13 and C14)

Column : Wakopak Navi C18-5, 4.6 mm × 250 mm (Flow rate : 1.0 mL/min.)R

Standard solution forwater quality test

Detergent

4

C12

C12

2

2

C13

C10 C11 LC12

LU

LU

LU
2

C11

LC11

4

4

C10

Mixture standard solutionof linear and branchedchain surfactants※

LC10

Water Quality Testing：CH3CN/H2O=65/35（v/v）、addition of 12.3 g of NaClO4 per 1000 mL

EluentDetection ：Fluorescence detection, ex. 221 nm, em. 284 nm※ Remarks
Linear Anionic Surfactant Standard Solutions (L) arecommercially available. They show behaviors different fromthose of the actual detergent components (the standard
solutions containing branched chains handled by Wako) in
HPLC analysis. Wakopak ® Wakosil AS-Aqua can recognize andseparate linear chains and branched chains.

3

HPLC analysis conditions

LC13

C13

LC14
C14

C14

0

0
0

10

20 Time (min)010

20 Time (min)

0

10

20

Time (min)Column : Wakopak Wakosil AS-Aqua, 4.6 mm × 250 mm (Flow rate : 0.7 mL/min.)
R

C14

4

4

2

Mixture standard solutionof linear and branchedchain surfactants※
LC10

C10

LC14

LU

LU

4

Detergent

LU

Standard solution for
C10
water quality test
C11
C12
C13
C14

2

2

0

0
0

10

20 Time (min)010

20 Time (min)

0

2

4

6

8

10

12

14 Time (min)Separation of anionic surfactant standard solutions and toluene

Although toluene may be contained in the extracted sample for measurement depending on the pretreatment method,
toluene and anionic surfactant are separately eluted in analysis using Wakopak® Wakosil AS-Aqua. It ensures analysis
without interference from toluene peak.
Sample: Addition of 1 μL/mL of toluene to 10 μg/mL of mixture standard solution (CH3OH), injection of 10 μL(Fig. 1) Fluorescence detection
8

Toluene

LU

6

C10
C11
C12

4

<Anionic surfactants>

C13

Sodium Decylbenzenesulfonate：C10
Sodium Undecylbenzenesulfonate：C11
Sodium Dodecylbenzenesulfonate：C12
Sodium Tridecylbenzenesulfonate：C13
Sodium Tetradecylbenzenesulfonate ：C14

C14

2

0

2

4

6

8

10

12

14
(min.)(Fig. 2) UV detection
C10
40

C12

mAU

ColumnToluene

C11

20：CH3CN/H2O=65/35（v/v）, addition of 12.3 g of NaClO4 per 1000 mL
Flow Rate ：0.7 mL/min. at 40℃
Detection ：(Fig. 1) Fluorescence detection, ex. 221 nm, em. 284 nm：(Fig. 2) UV detection, 255 nm
Injection ：10 mg/L
（CH3OH）、10 μL
Sample ：Standard solution for anionic surfactant test
(sodium alkylbenzene sulfonate: C10, C11, C12, C13 and C14)

C13
C14

0
0

2

4

6

8

10

：Wakopak® Wakosil AS-Aqua（4.6 mm × 250 mm）

Eluent12

14(min.)

Environmental Analytical Chemistry11GradePackage Size

Wako Cat. No.

Wakopak Wakosil AS-Aqua

4.6 mm × 250 mm (D)
4.6 mm × 250 mm (W)-

1 unit1 unit234-63281
230-63283

3

Wakopak® Navi C18-5

4.6 mm × 250 mm (D)
4.6 mm × 250 mm (W)-Water Quality Testing

HPLC Columns / Eluent
Product Name

Size

1 unit1 unit235-60531
231-60533

Wakosil® AS-Aqua Eluent

for HPLC

1L

238-02261

Package Size

Wako Cat. No.

10 each × 5

292-32251

10 each × 5

297-47451®

Solid-Phase Extraction Columns
Product Name

Presep®-C C18 (ODS)

Amount of filler(mg / cartridge)
900

Presep®-C C18 (ODS) (Short)
Presep Agri®

Gradefor Sample Pretreatment

470
500 / 6 mL

Presep®-C Agri (Short)for Pesticide Residue Analysis

220

50 each291-26851

10 each × 5

296-32651

Package Size

Wako Cat. No.

<Related Products>
Standards, Standard Solutions
Product Name

Anionic Surfactants Mixture Standard Solution

Grade

Sodium Decylbenzenesulfonate Standard Solution

Sodium Undecylbenzenesulfonate Standard Solution

Sodium Dodecylbenzenesulfonate Standard Solution
Sodium Tridecylbenzenesulfonate Standard Solution

for Water Analysis

Sodium Tetradecylbenzenesulfonate Standard Solution
Sodium p-n-Octylbenzenesulfonate Standard

Sodium p-n-Octylbenzenesulfonate Standard Solution

1 mL × 5A

013-20131

1 mL × 5A

195-13111

1 mL × 5A

192-13121

1 mL × 5A

199-13131

1 mL × 5A

196-13141

1 mL × 5A

193-13151

100 mg194-17101

1 mL

195-17131

Wako Cat. No.

Solvents (for LC / MS, for HPLC )Product Name

Grade

Package Size

for HPLC

25 mL

063-04192

Formic Acid (abt.99%)

for LC/MS

1 mL × 5A
50 mL

063-04533
067-04531

0.1vol% Formic Acid-Acetonitrile

for LC/MS

1L
3L

062-04721
068-04723

Acetonitrile

for HPLC

1L
3L

019-08631
015-08633

Acetonitrile

for LC/MS

100 mL
1L
3L

016-19854
012-19851
018-19853

Methanolfor HPLC

1L
3L

138-06473
132-06471

Methanolfor LC/MS

100 mL
1L
3L

132-14524
138-14521
134-14523

Distilled Water

for HPLC

1L
3L

046-16971
042-16973

Ultrapure Water

for LC/MS

1L
3L

214-01301
210-01303

Formic Acid (abt.99%)

12

Environmental Analytical Chemistryfor other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

3-2. Standards

Nonylphenol standards

Product Name

Grade

Nonylphenol (mixture of isomers)for Environment Analysis

p-n-Nonylphenol Standard

Package Size

Wako Cat. No.

100 mg148-09291

500 mg146-06791

Package Size

Wako Cat. No.

Water Quality Testing

onylphenol is an endocrine disruptor.
Addition of nonylphenol to environmental standards designed for protection of aquatic organisms (as part ofStandards based on the Basic Environment Law for Protection of Living Environment Related to Water Pollution (Japan)) iscurrently being discussed. “Solid-phase GC-MS” that can perform isomer-specific measurement is being considered as ananalysis technique recommended by the standards.

3

N<Related Products>
Product Name

Grade

1L
3L

011-19201
017-19203

1L
3L

043-28451
049-28453

Hexane 5,000

1L
3L

083-07911
089-07393

Sodium Sulfate

500 g

197-07125

Acetone 5,000
Dichloromethane 5,000

for Pesticide Residue & PCB Analysis

Linear Alkylbenzene Sulfonic acids and their salts (LAS)T

he Ministry of the Environment has issued their second reports on additional items for environmental regulation ofwater quality to protect aquatic organisms. (in Japan)
In this reports, the Ministry has announced that they are currently discussing adding environmental measures for linear
alkylbenzene sulfonic acids and their salts (LAS), in light of new data demonstrating the toxicity of these line of compounds.
Qualitative HPLC analysis has been proposed as the primary method of me, asuring LAS, and this product can be used as
an internal standard for such studies.

Example of analysis of Sodium p-n-Octylbenzenesulfonate

00

0

2

4

0

6

8

10 [min]＜HPLC 分析条件＞
Column：Wakopak® Navi C18-5 4.6mm×250mm
Eluent：0.1vol％HCOOH in CH3CN/
50mmol/L HCOONH4＝55/45(v/v)
Flow rate：1.0mL/min
Temperature ：40℃
Detection： UV 225nm
Injection：1mg/mL(CH3OH), 5μL

Standards
Product Name

Anionic Surfactants Mixture Standard Solution
Sodium p-n-Octylbenzenesulfonate Standard

Gradefor Water Analysis

Sodium p-n-Octylbenzenesulfonate Standard Solution

Package Size

Wako Cat. No.

1 mL × 5A

013-20131

100 mg194-17101

1 mL

195-17131

Analytical Columns
Product Name

Size

Package Size

Wako Cat. No.

Wakopak® Wakosil AS-Aqua

4.6 mm × 250 mm (D)
4.6 mm × 250 mm (W)

1 unit1 unit234-63281
230-63283

Wakopak® Navi C18-5

4.6 mm × 250 mm (D)
4.6 mm × 250 mm (W)

1 unit1 unit235-60531
231-60533

Environmental Analytical Chemistry13Aniline

Standard

Water Quality Testing3

Product Name

AnilineAssay

Package Size

Wako Cat. No.

99.0+% (Titration)

100 mL
500 mL

019-03991
019-03996

p-(1,1,3,3-Tetramethylbutyl)phenol (4-tert-Octylphenol)
Standard

Product Namep-(1,1,3,3-Tetramethylbutyl)phenol Standard

Grade

Package Size

Wako Cat. No.

for Environment
Analysis

500 mg208-14451

Grade

Package Size

Wako Cat. No.

for Environment
Analysis

500 mg049-26611

2,4-Dichlorophenol
Standard

Product Name

2,4-Dichlorophenol Standard

3-3. Musty-Odor Standard Analysis

Geosmin and 2-MethylisoborneolThe moldy odor produced by environmental pollution in rivers and lakes is due to metabolic products of various
abnormally proliferated actinomyces and algae. Geosmin in particular is considered to be most responsible foroffensive odors in tap water because it has a strong earthy odor (or moldy odor) in a trace amount. It is used as a reference
standard for analysis of geosmin.

Standards
Geosmin Standard

Product Name

Grade

Geosmin Standard Solution(0.1mg/ml Methanol Solution)

2-Methylisoborneol Standard

for Water Analysis

2-Methylisoborneol Standard Solution(0.1mg/ml Methanol Solution)

2-Methylisoborneol-Geosmin Mixture Standard Solution(each 0.1mg/ml Methanol Solution)

Package Size

Wako Cat. No.

20 mg

077-01911

1 mL

072-03421

20 mg

132-07071

1 mL

134-10581

1 mL

131-12431<Related Products>
Product Name

2,4,6-Trichloroanisole Standard
Sodium Chloride
Sodium Azide
Presep -C C18 (ODS)®

14

Environmental Analytical Chemistry

NotePackage Size

Wako Cat. No.

100 mg209-18901

500 g

192-10745

98.0+% (Titration)

25 g

195-11092

for Sample Pretreatment

10 each × 5

292-32251

for Water Analysis

for other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

4.

Food Analysis

4-1.

Melamine Standards

Structural Formula
Melamine

Cyanuric acid (isocyanuric acid)

NH2
N
H2N

OH

N

N
NH2

N

HO

N
OH

N

CAS No. : 108-78-1
C3H6N6=126.12

CAS No. : 108-80-5
C3H3N3O3=129.07

Ammeline

Ammelide

NH2
N
HO

Food Analysis

elamine occurs as monoclinic crystals and is used mainly as a raw material of melamine resin for decorative sheet,molded plateware, molded machines and electric parts, baking paint, and textile processing agent.
After the case of melamine contamination in pet foods in the U.S. in 2007, the Ministry of Health, Labour and Welfare has
instructed to perform a monitoring test for proteins (including gluten) made from rice from China and wheat and for flourand powder prepared using flour.

4

M

OH

N

N
NH2

N

HO

CAS No. : 645-92-1
C3H5N5O=127.10

N
N

NH2

CAS No. : 645-93-2
C3H4N4O2=128.09

Application using Wakosil 5NH2

GC/MS Application (TMS derivetized Melamin andthe related compounds)<HPLC Conditions>
Column: Wakosil 5NH2 (4.6×250 mm), 40℃
Eluent: CH3CN/5mM NaH2PO4 (pH7.0)=70:30 (v/v)
Flow Rate : 1.0mL/min.
Detection : UV210 nm
Sample: 1) Melamine
2) Isocyanuric Acid
3) Ammelide (1 mg/mL 0.1 M NaOH aq)
4) Ammeline (1 mg/mL 0.1 M NaOH aq) each 0.1mg/mL
Injection Volume : 10 μL<GC/MS Conditions>[GC]
Column: BPX-5 0.25 μm, 0.25 mm × 30 m
Column Temp : 75℃ (1 min) 15℃/min)
320℃ (2.67 min)Injection: 280℃
Carrier Gas: He 1.00 mL/min
Split Ratio: 1/30Injection Volume : 1 μL

[MS]
Ionization Mode : El
Interface: 290℃
Mode: SIM
Monitor Ion: 1) Isocyanuric Acid (m/z 345)
2) Ammelide (m/z 344)
3) Ammeline (m/z 328)
2) Melamine (m/z 342)

3
4

1.0
1

2

0.8

1

0.6
4

0.4
3
2

0.2

0.0
0

2

4

6

8

10

12

14

16

18

10.0

20 min19.0 minStandards of Melamine and the related compounds
Melamine Standard

Product Name

Isocyanuric Acid Standard
Ammeline Standard

Ammelide Standard

Grade

Package Size

Wako Cat. No.

for Food Analysis

100 mg132-15881

for HPLC

200 mg091-05311

for Food Analysis

100 mg012-22041

100 mg019-22051

Environmental Analytical Chemistry15

<Related Products>
Product Name

Note

Package Size

Wako Cat. No.

4.6 mm × 250 mm (D)
4.6 mm × 250 mm (W)

Size

-

1 unit1 unit

238-57691
234-57693

Diethylamine-

99.0+% (cGC)

25 mL
500 mL

047-01773
041-01776

Pyridine, Dehydrated-

99.5+% (cGC)

100 mL
500 mL161-18453
167-18455

Distilled Water--

500 mL2L

043-16785
047-16783

Acetonitrile 300-

for Pesticide Residue& PCB Analysis

1L
3L

015-11301
011-11303

2,4-Diamino-6-chloropyrimidine-

98.0+% (Titration)

25 g
100 g

043-22432
045-22431

Wakopak Wakosil 5NH2

Food Analysis

4®

4-2. A Clean up of Aflatoxins

A

FLAKING manufactured by HORIBA, Ltd. (Kyoto, Japan) has been developed
to extract aflatoxin from food such as nuts, grain and spices.
AFLAKING is an immunoaffinity column developed for cleanup of aflatoxins fromfood. With conventional immunoaffinity columns, it was required to dilute organic
solvent extracts to 2 % or so, and, as the result of this, turbidity was caused, andanalysis of spices, etc. was difficult. AFLAKING is resistant to the solvents of 20 %acetonitrile and 40 % methanol which are widely used for extraction and ensureseasy and quick cleanup of a wide range of food, such as nuts, cereals and spices.
It can clean up aflatoxins B1, B2, G1 and G2 simultaneously. In addition, it can clean
up aflatoxin M1. (M1 recovery: 87 %) It conforms to Notice No. 0728004 of
Pharmaceutical and Food Safety Bureau of the Ministry of Health, Labour andWelfare.

High resistance to organic solvents[Resistance to methanol]
Recovery (%)

Recovery (%)[Resistance to acetonitrile]
100
80
60
40
20
0

0

20

40

60

80

100
80
60
40
20
0

100

0

20

Acetonitorileconcentration

40

60

80

100

Methanol concentration (%)

B1

B2

G1

G2

RecoveryRoasted peanuts
Corn grits
Pearl barley
PaprikaB
B
G
G
Note) Recovery (%) in the case of addition of 16 ng/g of total aflatoxin
Description
AFLAKING

AFLAKING 25

Code No.

Product Name

AFLAKING 50

16

White sesame seeds

Environmental Analytical Chemistry

Red pepper

Turmeric

Coriander

Wako Cat. No. (Pkg. Size)

Package Size

Wako Cat. No.

25 unit308-34201

50 unit304-34203

for other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

5.

BiocideDimethylfumarate (DMF) Standard

A

Appearance
Solubility in acetone
Melting Point
Assay (cGC)

BiocideSpecification

5s of 1 May 2009 products containing a biocide Dimethylfumarate are prohibited from being placed or made available
on the European communities market.
Since 2007, sofas exported to the UK from China which contained dimethylfumarate (DMF), a biocide preventing moulds
that may deteriorate leather furniture or footwear during storage or transport in a humid climate have caused dermatitis in
consumers who have been in contact with these sofas.
Number of victims: several thousand (in the UK, France, Poland, Finland, Sweden, etc.)
DMF was most often contained in little pouches fixed inside the furniture or added to the footwear boxes. It thus
evaporated and impregnated the product, protecting it from moulds. However, it then also affected consumers who werein contact with the products. DMF penetrated through the clothes onto consumers’ skin where it caused painful skincontact dermatitis, including itching, irritation redness, and burns; in some cases, acute respiratory troubles are reported.
The dermatitis was particularly difficult to treat. The presence of DMF is thus a serious risk.
Method’s Quantification Limit (MQL): 0.1 mg/kg of the weight of the product or part of the product.

White, Crystals ~ crystalline powder
to pass test
101 ~ 105℃
99.0+%
Description

Dimethyl Fumarate Standard, 99.0+% (cGC)[IUPAC Name] Dimethyl (E)-butenedioate[CAS No.] 624-49-7; [Einecs No.] 210-849-0[Molecular Weight] 144.13[Molecular Formula] CH3OCOCH:CHCOOCH3

Grade

Package Size

Wako Cat. No.

for Household
Articles Test

100 mg041-31061

Reference: The EU regulation

http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2009:074:0032:0034:EN:PD

Environmental Analytical Chemistry

17

6.

Metal Standard Solutions

Metal Standard Solutions / JCSSW

Metal Standard
Solutions6

ako has been qualified as an institution for calibration (a licensed trader) of standard pH solutions, standard metal
solutions and standard ion solutions by the Minister of Economy, Trade and Industry in Japan and offers thestandard solutions traceable according to the National Metrology Standard. These products are provided with a calibration
certificate marked with JCSS to certify that it is traceable according to the National Metrology Standard and, at the sametime, attests to the supply of accurate and highly reliable reagent.

18

Product Name

Aluminium Standard Solution (Al 100)
Aluminium Standard Solution (Al 1000)
Antimony Standard Solution (Sb 100)
Antimony Standard Solution (Sb 1000)
Arsenic Standard Solution (As 100)
Arsenic Standard Solution (As 1000)
Barium Standard Solution (Ba 1000)
Bismuth Standard Solution (Bi 100)
Bismuth Standard Solution (Bi 1000)
Boron Standard Solution(B 1000)
Cadmium Standard Solution (Cd 100)
Cadmium Standard Solution (Cd 1000)
Calcium Standard Solution (Ca 100)
Calcium Standard Solution (Ca 1000)
Cesium Standard Solution(Cs 1000)
Chromium Standard Solution (Cr 100)
Chromium Standard Solution (Cr 1000)
Cobalt Standard Solution (Co 100)
Cobalt Standard Solution (Co 1000)
Copper Standard Solution (Cu 100)
Copper Standard Solution (Cu 1000)
Gallium Standard Solution (Ga 1000)
Indium Standard Solution (In 1000)
Iron Standard Solution (Fe 100)Iron Standard Solution (Fe 1000)
Lead Standard Solution (Pb 100)
Lead Standard Solution (Pb 1000)
Lithium Standard Solution (Li 1000)
Magnesium Standard Solution (Mg 100)
Magnesium Standard Solution (Mg 1000)
Manganese Standard Solution (Mn 100)
Manganese Standard Solution (Mn 1000)
Molybdenum Standard Solution (Mo 1000)
Nickel Standard Solution (Ni 100)
Nickel Standard Solution (Ni 1000)
Potassium Standard Solution (K 100)
Potassium Standard Solution (K 1000)
Rubidium Standard Solution (Rb 1000)
Selenium Standard Solution (Se 1000)
Sodium Standard Solution (Na 100)
Sodium Standard Solution (Na 1000)
Strontium Standard Solution (Sr 1000)
Tellurium Standard Solution (Te 1000)
Thallium Standard Solution (Tl 1000)
Tin Standard Solution (Sn 1000)
Vanadium Standard Solution (V 1000)
Zinc Standard Solution (Zn 100)
Zinc Standard Solution (Zn 1000)

Environmental Analytical Chemistry

Concentration (mg / L)

Solvent

Package Size

1,000

HNO3

100 mL

100
100

HNO3

100 mL

Wako Cat. No.
016-18271
016-15471

HCl

100 mL013-18281

100

1,000

HNO3 in Water,
pH 5.0 with HCl

100 mL013-15501

1,000
100

HNO3

HNO3

100 mL027-15321

1,000

Water

100 mL025-16581

1,000

HNO3

100 mL036-16171

1,000

1,000
100

HCl

HNO3

HNO3

100 mL

100 mL

100 mL
100 mL
100 mL013-15481

023-14201
021-12661
030-16211

100

HNO3

1,000

Water

100 mL030-21341

1,000

HNO3

100 mL030-16191

1,000
100

HNO3

HNO3

100

HNO3

100

HNO3

1,000

HNO3

1,000
1,000
1,000

HNO3
HNO3
HNO3

100

HNO3

100

HNO3

1,000

HNO3

1,000
1,000

HNO3
HNO3

100

HNO3

100

HNO3

1,000

HCl · HNO3

1,000

HNO3

1,000
1,000
100

HNO3
HNO3

HNO3

100 mL010-15491

100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL
100 mL036-17891
039-16161
037-16221
039-17901
033-16181
034-16231
033-16201
070-05781
092-05841
091-03851
094-03841
127-04301
124-04291
129-05221
136-13601
136-12121
139-12111
133-12131
130-14961
144-06471
147-06461

100

Water

100 mL162-19941

1,000

Water

100 mL

188-01951

1,000
1,000

Water
HNO3

100 mL
100 mL

100

Water

100 mL

1,000

HNO3

100 mL

1,000
1,000

Water

199-10831

199-13871

100 mL209-17921

HCl

100 mL202-16311

HNO3

100 mL

261-01431

HNO3

1,000

HCl · HNO3

1,000

HNO3

100

192-13861

191-12111

HCl

1,000
1,000

100 mL165-17471

100 mL
100 mL
100 mLfor other products, please visit the Wako Online Catalog

205-16301
221-01851
264-01421

https://labchem-wako.fujifilm.com/

7.
7-1.

Solvents
for Pesticide Residue and PCB Analysis

These products ensure safety as each contains no interfering substances in the 300-fold or 5000-fold concentrated
solution, and are ideal for the extraction of pesticides from the test substances and for purification.

Suitability for Pesticide Residue and PCB Analysis

Product Name

Grade

Acetone 5,000
Acetonitrile 300

for Pesticide Residue& PCB Analysis

Acetonitrile 5,000
Acetonitrile

for Tiuram Analysis

Benzene 300
Benzene 5,000
t-Butyl Methyl Ether 300
t-Butyl Methyl Ether 5,000
Chloroform 300
Chloroform 5,000
Cyclohexane 300
Cyclohexane 5,000

1L
for Pesticide Residue& PCB Analysis

Dichloromethane 5,000, 2-Methyl-2-butene added
Diethyl Ether 300
Diethyl Ether 5000-

Ethanol 5,000
Ethyl Acetate 300
Ethyl Acetate 5,000
Hexane 300

Methanol 5,000
Petroleum Ether 300
Petroleum Ether 5,000
Toluene 300
Toluene 5,000

1L
1L
3L
1L
1L
3L
1L
3L
1L
1L

Ethanol 300

Methanol 300

024-14351

1L

Dichloromethane 5,000

Hexane 5,000

1L

1L

Dichloromethane 300

Distilled Water, Hexane Washed

Wako Cat. No.
015-11281
011-11283
011-19201
017-19203
015-11301
011-11303
013-19401
019-19403
011-15281
017-15283
021-08631

for Pesticide Residue& PCB Analysis

1L
1L
3L
1L
3L
1L
1L
3L
1L
3L
1L
3L
1L
3L
1L
3L
1L
3L
1L
1L
1L
3L
1L
3L

Solvents

Acetone 300

Package Size
1L
3L
1L
3L
1L
3L
1L
3L
1L
3L
1L

7

The 300- and 5000-fold concentrated solution guarantee the followings :1 With the GC-ECD, the level of impurities does not exceed 1/2 of the peak of the organochlorine pesticide γ-BHC (20pg).
2 With GC-FPC, the level of impurities does not exceed 1/25 of the peak of the organophosphorous pesticide MPP (0.5ng)

028-14751
020-14831
039-11801
033-18641
038-16751
034-16753
036-18631
133-08841
139-08843
043-28451
049-28453
042-30011
050-04461

040-28461
040-28081
046-28083
056-04441
052-04443
053-07011
052-04421
058-04423
052-06981
058-06983
084-04761
080-04763
083-07911
089-07913
139-08821
135-08823
132-14161
138-14163
165-12971
162-20671
203-11601
209-11603
209-15581
205-15583

Environmental Analytical Chemistry

19

7-2.

for LC/MS

Liquid chromatography - mass spectrometry (LC/MS) is widely used in various fields including biological, food, andenvironmental analyses. In particular, recent breakthroughs in the development and upgrades of device interfaces have led
to the use of LC/MS in microanalyses of environmental pollutants and chemical metabolites, etc. Following products are
ideal LC/MS reagents to analyze trace components.
Product Name

Specifications

Package Size

Wako Cat. No.

1 mL × 5A
50 mL

014-20063
018-20061

Acetic Acid

· Suitability test for LC/MS analysis
performed· Reduced background noise

Assay (HPLC): 99.5+%
Absorbance (1 → 4,250 nm): max. 0.50
Absorbance (1 → 4,254 nm): max. 0.10
Fluorescence test: to pass test
Suitability for LC/MS analysis: to pass test

Acetonitrile· Suitability test for LC/MS analysis
performed· Guarantees noise level at
m/z 50~2,000· Use of aluminum caps
Reduced risks of slight amounts ofcontaminants from plastic caps.

Assay (cGC): 99.8+%
Density (20°C): 0.780 ~ 0.783 g/mL
Fluorescence test: to pass test
Suitability for LC/MS analysis: to pass test

100 mL
1L
3L

016-19854
012-19851
018-19853· Suitability test for LC/MS analysis
performed· Reduced background noise

Assay (HPLC): 99.5+%
Solubility in water: to pass test
Absorbance (1 → 4,254 nm): max.1.00
Fluorescence test: to pass test
Suitability for LC/MS analysis: to pass test

1 mL × 5A
50 mL

063-04533
067-04531

0.1vol% Formic
Acid-Acetonitrile· Suitability test for LC/MS analysis
performed· Ready-to-Use eluent

Absorbance (200-400 nm): to pass test
Fluorescence test: to pass test
Water: max. 0.05%

1L
3L

062-04721
068-04723

Methanol· Suitability test for LC/MS analysis
performed· Guarantees noise level at
m/z 50~2,000· Use of aluminum caps
Reduced risks of slight amounts ofcontaminants from plastic caps

Assay (cGC): 99.7+%
Density (20°C): 0.789~0.792 g/mL
Fluorescence test: to pass test
Suitability for LC/MS analysis: to pass test

100 mL
1L
3L

132-14524
138-14521
134-14523

2-Propanol· Suitability test for LC/MS analysis
performed

99.7+% (Capillary GC)
Density (20°C): 0.784~0.787 g/mL
Fluorescence test: to pass test
Suitability for LC/MS analysis: to pass test

1L
3L

168-25531
164-25533

Ultrapure Water· Decreased total organic carbon levels· Guarantees the absorbance andfluorescence tests· Use of specially processed glass
containers / aluminum caps

Density (20°C): 0.997 ~ 0.999 g/mL
Refractive index nD20: 1.332 ~ 1.334
Absorbance (210~400 nm): max. 0.01
Fluorescence test: to pass test
Total organic carbon (TOC): max. 4 ppb

1L
3L

214-01301
210-01303

7
Solvents

Features

Formic Acid(abt. 99%)

7-3.

for HPLC

Followings are high purity HPLC solvents. For improved reproducibility and detection sensitivity of chromatography, thestability of the analyte, stability of the mobile phase, clogging in the line and filter, sensitivity and stability of the detector
have been considered, and the products have guaranteed quality with respect to change in refractive index due to water,
peroxides, nonvolatile matters, or impurities in the solvent, UV absorption, and fluorescent substances, etc.
Product Name

20

Package Size

Wako Cat. No.

Acetone1L
3L

014-08681
010-08683

Acetonitrile

1L
3L

019-08631
015-08633

Benzene1L

025-06691

1-Butanol

1L

023-10801

t-Butyl Methyl Ether

1L

024-12771

Chloroform

1L
3L

033-08631
039-08633

Chloroform, Amylene added

1L
3L

031-20531
037-20533

Environmental Analytical Chemistryfor other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

Product Name

Package Size

Wako Cat. No.

o-Dichlorobenzene

1L

046-18671

Dichloromethane

1L
3L

136-06751
132-06753

N,N-Dimethylformamide

1L
3L

042-20621
048-20623

1,4-Dioxane

1L

042-16691

Distilled Water

1L
3L

046-16971
042-16973

Ethanol (99.5)

1L
3L

056-03341
052-03343

Ethyl Acetate

1L
3L

057-03371
053-03373

Heptane1L

085-03691

100 mL
500 mL085-06991
087-06995

Hexane1L
3L

084-03421
080-03423

Methanol

1L
3L

138-06473
132-06471

1-Propanol

1L

162-13461

2-Propanol

1L
3L

165-09161
161-09163

Tetrahydrofuran, with Stabilizer

1L
3L

200-19391
206-19393

Tetrahydrofuran, Stabilizer Free

1L
3L

209-06811
205-06813

Toluene1L

209-06791

0.1 vol% Trifluoroacetic Acid - Acetonitrile

1L
3L

206-16451
202-16453

2,2,4-Trimethylpentane

1L

207-06731

1-Methyl-2-pyrrolidone

1L

130-17641

1,1,1,3,3,3-Hexafluoro-2-propanol

Solvents

033-08511
039-08513

7

Cyclohexane

1L
3L

Solvents and pH Adjusting Reagents
Package Size

Wako Cat. No.

25 mL010-19112

25 mL

063-04192

Phosphoric Acid

25 mL

162-20492

Trifluoroacetic Acid

1 mL × 5A
5 mL × 5A

206-10731
206-10736

Package Size

Wako Cat. No.

100 mL011-21031

1 mol/L Ammonium Dihydrogenphosphate Solution

100 mL015-21051

1 mol/L Ammonium Acetate Solution

100 mL018-21041

0.25 mol/L Potassium Dihydrogenphosphate Solution

500 mL

168-19965

0.25 mol/L Sodium Dihydrogenphosphate Solution

500 mL

197-12135

Acetic Acid

Product Name

Formic Acid (abt. 99%)

Eluents for HPLC Analysis
Product Name

1 mol/L Ammonium Formate Solution

Environmental Analytical Chemistry

21

8.

Pretreatment Columns -Presep® SeriesT

he solid-phase extraction method for pretreatment of samples is usedwidely in various fields, including pharmaceutical, food and environmental
analyses, because the method is simple and uses smaller amounts of solvents.
In addition, the method has various advantages, for example, it can treat many
samples simultaneously in a short time.
Presep® series are categorized by their shapes; one is “Presep®-C type” which
has a cartridge shape with closed both ends, the other is “Presep® syringe type”
with one open and one closed ends.
Presep®-C

Pretreatment Columns

8(Short)Presep® Syringe Typewith one end open

Features

1. The columns can be used for solid-phase extraction by any of the compression and decompression methods.
(Presep®-C Type)
2. Some columns can be connected depending on the kind and amount of filler. (Presep®-C Type)
3. High flow velocity obtained by the sharp filler particle size distribution.
4. High recovery realized by the fillers designed for solidphase extraction.
5. High reproducibility ensured by strict quality control.

List of Fillers
Kind

FillerParticle size (μm)

C18 (ODS)

C18(ODS)-bonded silica gel

63 ~ 212

NH2

Aminopropylsilanized silica gel

38 ~ 63

Silica gel

Crushed silica gel

75 ~ 150

Alumina

Basic (pH 9) alumina

44 ~ 149

Florisil®

MgO3Si75 ~ 150

Na2SO4
Diatomaceous Earth, Granular

Sodium sulfate (anhydrous)
Granular diatomaceous earth

500 ~ 1400

Polyamide C-200

Polyamide resin

75 ~ 150

RPP

Styrene-divinylbenzene-methacrylate
polymer30, 60RPP-SAX40 ~ 48

RPP-WAX

Divinylbenzne-methacrylate polymer
anion-exchange group bonding

DEA
QA
CM

Diethylaminoethyl Cl type
Trimethylaminoethyl Cl type
Carboxymethyl Na type

S

Sulfonyl propyl Na type

PFC/PFC-Ⅱ

Divinylbenzene-methacrylate polymer

50

Agri

Styrene-divinylbenzene-methacrylate
polymer50

DNPH
Ozone scrubber
Multilayer silica gel
Activated carbon-blended
silica gel
Activated carbon-impregnatedsilica gel
PolyChelate
Dehydration

22

Presep®-C

Silica gel coated with
2,4-dinitrophenylhydradineHigh-purity potassium iodine
10% silver nitrate-silica gel, 22% sulfate
silica gel, etc.
Activated carbon-blended silica gel

60

45 ~ 90

Short:150 ~ 450
Long:75 ~ 150-

Uses and characteristics
Reversed phase partition : Separation ofhydrophobic substances in water-soluble
samples
Removal of acidic compounds, such asorganic acids and fatty acids
Normal phase adsorption: Separation oflow- to medium-polarity components from
nonaqueous solutions
Removal of interfering substances frompesticides and environment samples
Removal of lipid and pretreatment of pesticide
residue in food
Dehydration
Desolventization
Pretreatment of paeoniflorin in Kakkonto
extract
Trapping of high-polarity substances whichcannot be adsorbed by normal ODS
Pretreatment of biological samples
Selective extraction of acidic compounds andextraction of drug products and their metabolites from biological samples (urine and blood)
Polymer-based weakly basic anion exchange
Polymer-based strongly basic anion exchange
Polymer-based weakly acidic cation exchange
Polymer-based strongly acidic cationexchange
Pretreatment for analysis of organofluorinecompounds (PFCs)
Trapping of high-polarity substances whichcannot be adsorbed by normal ODS
Pretreatment for analysis of pesticide residue
Derivatization after adsorption of aldehydes
and ketones
Removal of ozone-

Pretreatment for analysis of dioxins-

Trapping of metal elements-

Dehydration

Activated carbon-impregnated silica gel
Chelate resin modified withcarboxymethylated polyethylenimine.
Hydrophobic Teflon membrane filter

Environmental Analytical Chemistryfor other products, please visit the Wako Online Catalog

https://labchem-wako.fujifilm.com/

Presep® Series ; Solid-Phase Extraction Columns
Product Name

Presep® (Luer Lock) Polyamide C-200 Type M
Presep®-C C18 (ODS) (Short)Amount of filler(mg/cartridge)

Use

Package Size

Wako Cat. No.

2000 / 25 mL

for Sample Pretreatment(crude drug)

10 each × 5

298-33571

10 each × 5

297-47451

470

Presep -C C18 (ODS)

900

10 each × 5

292-32251

Presep® C18 (ODS)

2000 / 25 mL

100 each296-34091

Presep® C18 (ODS) Type M

5000 / 25 mL

20 each100 each

293-48553
297-48556®

10 each × 5

299-48751

Presep -C NH2820

10 each × 5

295-48851

Presep®-C Silica Gel

800

50 each294-31851

Presep -C Alumina

1700

10 each × 5

290-32051

Presep®-C Florisil®

800

10 each × 5

290-31951

1000 / 6 mL

10 each × 5

291-44051

2300

10 each × 5

296-32151

100 each
100 each292-35051
298-35151

100 each291-33561
297-41851®

Presep Florisil®

Presep®-C Na2SO4
Presep® Diatomaceous Earth, Granular1000 / 6 mL2000 / 15 mL

Presep® (Luer Lock) Diatomaceous Earth,
Granular Type M

4500 / 25 mL

for Sample Pretreatment

Presep®-C RPP (Short)

190

10 each × 5

Presep®-C RPP (Long)360

10 each × 3

293-41951

Presep® RPP

60 / 3 mL
200 / 6 mL
500 / 6 mL

10 each × 5
10 each × 5
10 each × 5

294-36851
290-36951
290-37051

Presep® RPP-SAX

60 / 3 mL

10 each × 10

297-33301

Presep RPP-WAX

60 / 3 mL

10 each × 10

291-33941

Presep DEA

250 / 6 mL

10 each × 5

292-61701

Presep® QA

250 / 6 mL

10 each × 5

296-61601

Presep CM

250 / 6 mL

10 each × 5

298-61801

Presep® S

250 / 6 mL

10 each × 5

294-61901

Presep PFC-II

60 / 3 mL

10 each × 10

291-33441

10 each × 5

297-49651

®
®

®

®

Presep®-C PFC (Short)
Presep -C Agri (Short)®

Presep®-Agri

220
220
500 / 6 mL

Presep®-C DNPH (Short)

400

Presep®-C DNPH

800

Presep -C Ozone Scrubber

1300

®

for Sample Pretreatment(PFCs)
for Pesticide Residue
Analysisfor Collection of Aldehydes

Presep Multilayer Silica Gel®

Presep® Active Carbon-impregnated Silica Gel

1000

Presep Acitive Carbon-impregnated Silica Gel(Reverse Column)

1000

®

Presep® PolyChelate
Presep Dehydration, 48WELL PLATE®

for Dioxins Analysis

10 each × 5

296-32651

50 each291-26851

20 each291-43951

20 each290-34251

20 each293-40351

5 each295-41651

10 each293-41451

5 each

297-43051

250 / 3 mL

for Trapping of Metal
Elements

10 each × 5

296-33491

3 mL

for Organic Synthesis

10 each299-44351

Environmental Analytical Chemistry

Pretreatment Columns

400

®

8

Presep®-C NH2 (Short)

23

9.

High-sealed Storage Bottles

There high-performance hermetic container are ideal for storing volatile solvents, agrochemicals, dioxins,
and other types of standard solutions. Specially designed to be completely airtight, the Perfluoro O-ring
between the mouth and the cap ensures preventing swelling of the container affected by expansion andcontraction due to temperature fluctuations and organic solvent vaporization. This enables long storage ofvarious types of solvents at temperatures between ambient (room temperature) and the freezer (-20°C).

Featuresl Provides 99+% hermeticity when organic solvents are stored at room temperature or at freezer
temperature as low as -20°C, for up to three months.
l Comes in sizes of 1 mL, 2 mL, 5 mL, and 10 mLl A microsyringe is available for drawing samples directly from the specially designed upper extraction cap

Applicationsl Storage of commercial standard solutions removed from ampoules
l Long storage of reagent samples

Hermeticity tests for various solvents

100.0
99.9
99.8
99.7
99.6
99.5
99.4
99.3

0 weeks 1 weeks 2 weeks 3 weeks 1 month 2 months 3 months

Hexane hermeticity

100.1

Vaporization rate (%)

Acetone hermeticity

100.1

Vaporization rate (%)

Vaporization rate (%)

Used Wako’s high-performance hermetic containers (10 mL) and their equivalent products by Company A (10 mL). Added
acetone, hexane, and diethyl ether (10 mL each) respectively, sealed, and stored both at room temperature and at freezer
temperature (-20°C). Measured the mass of each content at various intervals from a week to 3 months after the seal.

100.0
99.9
99.8
99.7
99.6
99.5

0 weeks 1 weeks 2 weeks 3 weeks 1 month 2 months 3 months

Wako’s container, room temperature storage (n = 3)
Equivalent product by Company A, room temperature storage

High-performance hermetic design*1

To avoid lower hermeticity due to expansion andcontraction affected by temperature fluctuations, ascrew-top is equipped in the inner wall of the mouthcomponent.*2
In addition, solvent resistant
Perfluoro O-ring, which istolerant of temperaturefluctuations is used between themouth and the cap.

[Materials]

100.5

Diethyl ether hermeticity

100.0
99.5
99.0
98.5
98.0

0 weeks 1 weeks 2 weeks 3 weeks 1 month 2 months 3 months

Wako’s container, freezer temperature storage (n = 3)
Equivalent product by Company A, freezer temperature storage

Direct sample withdrawal with a microsyringe

The specially designed withdrawal cap allows direct sample withdrawal
with a microsyringe without compromising hermeticity.

[Recommended syringes]<Needle length>

Syringes with the needles of the following lengths willreach the bottom of the bottle.
l For glass barrel OD of ≤ 7.6 mm, needle length
should be ≥ 50 mm*3l For glass barrel OD of ≥ 7.6 mm, needle length
should be ≥ 70 mm*3

<Needle point>

Container body: Glass
Cap: Teflon
O-ring: Perfluoro and Vitonl LC tip (with a 90° angle cut)

*1: Japan Design Patent No. 1425874*2: Patent Publication Number 2012-192978 (Japan)*3:When glass barrel OD exceeding 7.6 mm are used, thesyringe cannot reach the end of the extraction cap. Pleaseconfirm the size of the OD when using syringes withvolumes of ≥25 μL.

Product Name

High-sealed Storage Bottle, Brown

Bottle Size
1 mL
2 mL
5 mL
10 mL

1 mL,
2 mL
V-shapedbottomPackage Size
1 bottle
1 bottle
1 bottle
1 bottle

5 mL,
10 mL
Flat
bottomWako Cat. No.
296-34731
293-34741
297-34761
294-34771■ Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.
■ Please visit our online catalog to search for other products from Wako : https://labchem-wako.fujifilm.com/■ This brochure may contain products that cannot be exported to your country due to regulations.
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