ࣂ࢖࣑࢜ ࢿࣛࣜࢮ࣮࣮ࢩࣙࣥࢆ฼฼⏝ࡋࡓᆅᆅ⇕㈨※ࡢ᭷᭷ຠ฼⏝ᇶᇶ┙ࡢ๰ᡂ◊◊✲௦⾲⪅஑ᕞ኱Ꮫ኱Ꮫ㝔㎰ᏛᏛ◊✲㝔 ⏕࿨ᶵ⬟⛉Ꮫ⏕Ꮫ㒊㛛 ㅮᖌᖌ ᅵᒃඞᐇᐇ

1. ⥴ゝ
⥴ ㏆ᖺ
ᖺࠊCO2 ᤼ฟᢚไ࡟㈨ࡍࡿ㠉᪂ⓗⓗᢏ⾡ࡢ๰ฟฟࡀႚ⥭ࡢㄢ
ㄢ㢟࡜࡞ࡗ࡚࡚࠸ࡿࠋCO2 ᤼ฟᢚไࡢࡓࡵ࡟ࡣ
ࡣࠊ͆෌⏕ྍ⬟⬟࢚ࢿࣝࢠ࣮࣮͇࠾ࡼࡧ͆㠉᪂ⓗ࡞࡞࢚ࢿࣝࢠ࣮
࣮㧗ᗘ฼⏝ᢏ
ᢏ⾡͇ࡀ୙ྍḞ࡞ㄢ㢟㢟࡛࠶ࡿࠋ᪂࢚࢚ࢿࣝࢠ࣮฼
฼⏝➼ࡢಁ㐍㐍࡟㛵ࡍࡿ≉ูᥐ⨨ἲἲ㸦RPS ἲ㸧࡛ࡣࠊ࡛෌⏕ྍ⬟࢚ࢿࣝ
ࣝࢠ࣮࡜ࡋ࡚
࡚ኴ㝧ගࠊ㢼ຊຊࠊᆅ⇕ࡲࡓ
ࡓࡣࣂ࢖࣐࢜
࣐ࢫ࡞࡝ࡀᣦ
ᣦᐃࡉࢀ࡚࠸
࠸ࡿࠋⓎ㟁ࡣ෌⏕⏕ྍ⬟࢚ࢿࣝ
ࣝࢠ࣮ࡢ୰࡛
࡛ࡶ CO2 ᤼ฟฟ㔞ࡀ 1KWWh ࠶ࡓࡾ 155g ࡛࠶ࡾ㸦㸦ⅆຊ;
ᆅ⇕Ⓨ742gࠊኴ㝧ග; 53gࠊ㢼ຊ;5229g㸧
ࠊᡃࡀᅜᅜࡢ⥲ᆅ⇕㈨㈨※㔞ࡣ 2,347 ୓ KWhh㸦࠺ࡕᅜ❧❧බᅬಖㆤᆅ༊࣭≉ูᆅᇦ௨௨እ࡛ࡢᆅ⇕⇕㈨※㔞ࡣ 4254 ୓ KWhh㸧࡛࠶ࡿࠋࡲࡓࠊᆅ⇕⇕ࡣ௚≉ูಖࡢ⮬↛࢚ࢿࣝࢠࢠ࣮࡜ẚࡋ࡚࡚ࠊ✌ാ⋡ࡀࡀ㧗ࡃ㸦70%%ࠊኴ㝧ග; 12%ࠊ㢼ຊ;120%㸧ࠊᆅ⇕⇕※ࡀᅜෆ࡟୓㐢࡞ࡃᏑᅾࡍࡿࡓࡓࡵࠊ᪥↷㔞ࡸࡸ㢼㔞ࡢᑡ࡞࠸ᆅᇦ࡛࡛ࡶⓎ㟁ྍ⬟⬟࡞Ⅼ࡞࡝⣧⣧ᅜ⏘࢚ࢿࣝࣝࢠ࣮࡜ࡋࡋ࡚᭷ᮃ࡛࠶࠶ࡿࠋࡋ࠿ࡋ࡞࡞ࡀࡽࠊࡇࢀࢀࡲ࡛ࡢᅜࡢࡢ࢚ࢿࣝࢠ࣮࣮ᇶᮏᡓ␎࡛࡛ࡣࠊᆅ⇕ࡣ᪂࢚ࢿࣝࣝࢠ࣮࡟ྵࡲ
ࡲࢀࡎࠊRPSS ἲ࡛ㄆᐃࡉࡉࢀࡿ࡟⮳ࡗࡗ࡚ࡶ᪋⾜௧௧ୖ࡛ࠊࠕ⇕⇕Ỉࢆⴭࡋࡃῶᑡࡉࡏࡏ࡞࠸ࡇ࡜ࠖࠊࠕࣂ࢖ࢼ࣮ࣜⓎ㟁࡟㝈㝈ࡿࠖ࡜࠸ࡗࡗࡓไ㝈ࡀㄢㄢࡉࢀ࡚࠸ࡿࡿࠋࡇࡢ⌮⏤࡜ࡋ࡚ࠊ ἨỈᯤῬ࡬ࡢᠱᛕࡀ
ࡀᣲࡆࡽࢀࡿࡿࡓࡵࠊⓎ㟁㟁࡟౑⏝ࡋࡓࡓ⇕Ỉࡣᆅୗୗ࡟㑏ඖࡉࢀࠊ෌฼⏝ࡉࢀࡿࠋ ࡜ ࡇࢁࡀࠊᆅᆅ㈓␃ᒙ୰ࡢ⇕⇕
ୗ㈓Ỉ࡟ ࡣ㧗⃰ᗘࡢࡢࢩ ࣜ ࢝ (SiO2) ࡀྵࡲ ࢀ࡚࠾ࡾࠊࢀࡀᆅ⇕Ⓨ㟁㟁ࡇࢀࡢ࣎ ࢺࣝࢿࢵࢡࢡ࡚ࡢ୍ ࡘ࡜࡞ࡗ࡚ࡣ࠸ࡿ ࠋࢩࣜ࢝ࡣẆ୰࡟᭱ࡶከከᆅẆࡃᏑᏑᅾࡍࡿ≀㉁㉁࡛࠶ ࡾࠊỈ࡟ࡣࡣࢣ࢖ 㓟࡜ࡋ࡚⁐⁐ゎࡋࠊࡑࡢ㣬࿴࿴⃰ᗘࡣᗘᗘ࣭ᅽຊ࣭pHH ࡟౫Ꮡࡍࡍࡿࠋࢩࣜ࢝ࡣ㸫㸫ᗘ࣭ᅽᅽຊࡢపୗ࡟࡟ࡼࡾ㐣㣬࿴࡟࡞ࡿ࡜⬺Ỉ⦰ྜࡋࠊ࣏࣐࣮ࣜࢆᙧᡂࡍࡿࡇ࡜࡛ࢥࣟ࢖ࢻ࡜࡞ࡾỈ⁐ᾮ୰࡟Ᏻᐃ࡟Ꮡᅾࡍࡿࠋࡼࡗ࡚㐣㣬࿴ࢩࣜ࢝⁐ᾮ࠿ࡽᅛయ≧ࢩࣜ࢝ࡣỿ㝆ࡋ࡟ࡃ࠸ࠋ࡜ࡇࢁࡀᆅ⇕ᖏ࡛ࡣࠊ㧗 ࣭㧗ᅽୗ࡛ࢩࣜ࢝ࡀ㣬࿴ࡋࡓ⇕Ỉࡀᄇฟࡋࠊᆅ⾲௜㏆ࡢࢣ࢖㓟⃰ᗘࡣ㐣㣬࿴࡟㐩ࡍࡿࠋࡇࡢࡼ࠺࡞ࢩࣜ࢝ࡢ໬Ꮫ≧ែࡢࡓࡵࠊᆅ⇕Ⓨ㟁ᡤࡢࣃ࢖ࣉࣛ࢖ࣥ୰࡛ࡣࢩࣜ࢝ỿẊ≀࡛࠶ࡿࢩࣜ࢝ࢫࢣ࣮ࣝࡀᛴ㏿࡟ᙧᡂࡉࢀࠊ⇕ຠ⋡ࡢపୗࡸࣃ࢖ࣉࡢ㛢ሰࢆㄏ㉳ࡋࠊⓎ㟁ຠ⋡ࡢపୗࠊ࣓ࣥࢸࢼࣥࢫ㈝⏝ࡢ㧗㦐ࠊ⇕Ỉࡢ෌฼⏝ࡢጉࡆ࡜࡞ࡗ࡚࠸ࡿ㸦ᅗ
1㸧ࠋࢩࣜ࢝ࢫࢣ࣮ࣝᙧᡂࡣᅽຊࡸᗘࠊ௚ࡢඹᏑ㔠ᒓ࢖࢜ࣥ࡜ࡢ┦஫స⏝ࢆ⪃៖ࡋࡓ↓ᶵ໬Ꮫⓗ࡞ᙧᡂ࣓࢝ࢽࢬ࣒࡛ࡣㄝ᫂୙ྍ⬟࡛ࠊࢩࣜ࢝ࢫࢣ࣮ࣝᙧᡂᶵᵓࡣᮍࡔᮍゎ࡛᫂࠶ࡿࠋࡲࡓࠊࢩࣜ࢝ࢫࢣ࣮ࣝࡣ㕲㗰ᴗ࡞࡝ࡢ㏦Ỉタഛ࡛ࡶ⇕஺᥮ჾࠊ㓟Ὑࣛ࢖ࣥࠊ⇕ᘏຍ⇕⅔࡞࡝࡛Ⓨ⏕ࡋࠊ໬Ꮫ⸆ရ࡟ࡼࡿ㝖ཤࡀ⾜ࢃࢀ࡚࠸ࡿࡀࠊ⸆ရởᰁ࡟ࡼࡿ⎔ቃ㈇Ⲵ
ࡀᠱᛕࡉࢀࠊࡼࡾ⎔ቃ࡟ࡸࡉࡋ࠸ᑐฎἲࡀồࡵࡽࢀ࡚࠸ࡿࠋ ࡜ࡇࢁࡀࠊࡇࡢࡼ࠺࡟῝้࡞㞀ᐖࢆࡶࡓࡽࡍࢩࣜ࢝ࢫࢣ࣮࡛ࣝ࠶ࡿࡀࠊࡑࡢᙧᡂᶵᵓ࡟ࡘ࠸࡚ࡢ▱ぢࡣṤ࡝࡞࠸ࠋ㏆ᖺࠊ⏕≀ࡀ↓ᶵ㔠ᒓ࢖࢜ࣥࢆ㞟✚ࡋ㖔≀໬ࡍࡿࣂ࢖࣑࢜
ࢿࣛࣜࢮ࣮ࢩࣙࣥ (biomineralization; ⏕≀㖔໬⌧㇟)ࡀὀ┠ࡉࢀ࡚࠾ࡾࠊࢩࣜ࢝ࢫࢣ࣮ࣝᙧᡂ࡟ࡶᚤ⏕≀ࠊ≉࡟⪏⇕ᛶᚤ⏕≀ࡢ㛵୚ࡀ᥎ࡉࢀࡓࠋࡲࡓࠊᚤ⏕≀ࢆ⏝࠸ࡓ㈨※ᅇ཰ἲ࡜ࡋ࡚ࣂ࢖࣮࢜ࣜࢳࣥࢢ࡞࡝ࡢᡭἲࡀ◊✲ࡉࢀ࡚ࡁ࡚࠾ࡾࠊᚤ⏕≀࡜㔠ᒓඖ⣲࡜ࡢ㛵㐃ࡣᛴ⃭࡟ὀ┠ࢆᾎࡧࡿࡼ࠺࡟࡞ࡗࡓࡀࠊࡑࡢ┦஫స⏝ࠊ≉࡟⏕⫱ᚲ㡲ඖ⣲࡛࡞࠸㔠ᒓ࡜⏕≀࡜ࡢ㛵㐃࡟ࡘ࠸࡚ࡣࠊᮍࡔᮍゎ᫂࡞Ⅼࡀከ࠸ࠋࡑࡇ࡛ᮏ◊✲࡛ࡣࠊᆅ⇕Ⓨ㟁ᡤ࡛⏕ࡌࡿࢩࣜ࢝ࢫࢣ࣮ࣝࢆᑐ㇟࡜ࡋࡓ㧗ᗘዲ⇕ᛶ⣽⳦࡟ࡼࡿࣂ࢖࣑࢜ࢿࣛࣜࢮ࣮ࢩࣙࣥࡢᙧᡂᶵᗎࢆ᫂ࡽ࠿࡟ࡍࡿ࡜ඹ࡟ࠊࡇࢀࡽࢆ⏝࠸ࡓᆅ⇕㈨※ࡢ฼ά⏝ἲࡢ㛤Ⓨࢆᶍ⣴ࡋࡓࠋ

2. ᐇ㦂᪉ἲ
2. 1. ౑⏝⳦ᰴ࠾ࡼࡧᇵ㣴᮲௳ ᮏᐇ㦂࡛ࡣࠊ◊✲௦⾲⪅ࡢࢢ࣮ࣝࣉࡀᆅ⇕Ⓨ㟁ᡤࡼࡾ༢㞳ࡋࡓ㧗ᗘዲ⇕ᛶ⣽⳦
Thermus thermophilus TMY (JCM 10668)1) ࠊ࠾ࡼࡧࠊࢤࣀ࣒㓄ิࡀゎㄞࡉࢀ࡚࠸ࡿ T.
thermophilus ࡢᇶ‽ᰴ HB8 ࢆ୺࡟⏝࠸ࡓࠋࡇࢀࡽ⳦ᰴࢆ TM ᾮయᇵᆅ࠾ࡼࡧ TM ᅛయᇵᆅࢆ⏝࠸ࠊ70Υ࡛ᇵ㣴ࢆ⾜ࡗࡓࠋᾮయᇵ㣴ࡣ᣺࡜࠺㸦150 rpm㸧ᇵ㣴ࢆ⾜࠸ࠊቑṪ᭤⥺ࡣ OD660 ࡢ್ࢆ

ᐃࡋ࡚స〇ࡋࡓࠋࡲࡓࠊ⣽⳦࡟ᑐࡍࡿࢩࣜ࢝ࡢᙳ㡪ࢆ᳨ࡍࡿ㝿ࡣࠊ

TM ᾮయᇵᆅ࡟࣓ࢱ⌛㓟ࢼࢺ࣒ࣜ࢘ࢆ㐺ᙜ࡞⃰ᗘ࡜࡞ࡿࡼ࠺ῧຍࡋࡓࡶࡢࢆ⏝࠸ࡓࠋ
2. 2. ⳦య࠿ࡽࡢ⏕య㧗ศᏊࡢศ㞳࣭⢭〇 ⳦య࠿ࡽࡢ DNA ࡢᢳฟ࣭⢭〇ࡣࠊᑐᩘቑṪᮇᚋᮇࡢ⳦యࢆ㐲ᚰศ㞳ᶵ࡟࡚㞟⳦ᚋࠊ㸫㸫ᖖἲ࡟ᚑ࠸⾜ࡗࡓࠋᚓࡽࢀࡓࢤࣀ࣒ DNA ࡣ PCR ཯ᛂࡢ㗪ᆺ࡜ࡋ࡚฼⏝ࡋࡓࠋࡲࡓࠊ
⳦య࠿ࡽࡢ඲ RNA ࡢᢳฟ࣭⢭〇ࡣࠊᇵ㣴᫬㛫ẖ࡟⳦యࢆᅇ཰ᚋࠊ࣍ࢵࢺࣇ࢙ࣀ࣮ࣝἲ
࠾ࡼࡧ RNeasy kit㸦࢟࢔ࢤࣥ♫〇㸧ࢆే⏝ࡋ࡚⾜ࡗࡓࠋᚓ⳦యࢱࣥࣃࢡ㉁ࡣࠊᅇ཰ࡋࡓ⳦యࢆ㉸㡢Ἴ◚○ᚋࠊ㉸㐲ᚰศ㞳࡟࡚ศ㞳ࡋࡓࡶࡢࢆ⏝࠸ࠊSDS-PAGE ࡲࡓࡣ 2D-PAGE➼࡟ࡼࡾศ⏬ࡋࡓࠋ
2. 4. PCR ቑᖜ࡜ቑᖜ᩿∦ࡢࢡ࣮ࣟࢽࣥࢢ ᢳฟ࣭⢭〇ࡋࡓࢤࣀ࣒ DNA ࢆᇶ࡟┠ⓗࡢ᩿∦ࢆቑᖜࡍࡿࡓࡵ࡟タィࡋࡓࣉࣛ࢖࣐࣮ࢭࢵࢺࢆ⏝࠸ࠊLA Taq ࣏࣓࣮ࣜࣛࢮ㸦ࢱ࢝ࣛࣂ࢖࢜♫〇㸧࡟ࡼࡾቑᖜࡋࡓࠋቑᖜࡋࡓ᩿∦ࢆ pTA2 ࣋ࢡࢱ࣮㸦ᮾὒ⣳〇㸧࡟⤌ࡳ㎸ࡳࠊ኱⭠⳦ JM109 ࡟ᙧ㉁㌿᥮ࡋࡓࠋᚓࡽ
ࢀࡓᙧ㉁㌿᥮య࠿ࡽࣉࣛࢫ࣑ࢻࢆᢳฟࡋࠊᤄධ DNA ᩿∦ࡢሷᇶ㓄ิࢆ 3130 GeneticAnalyzer 㸦Applied Biosystems ♫〇㸧ࢆ⏝࠸࡚ゎᯒࡋࡓࠋ
2. 5. ㌿෗ゎᯒ ⢭〇ࡋࡓ RNA ࢆ⏝࠸ࠊRT-PCR ࡟ࡼࡾ cDNA ࣛ࢖ࣈ࣮ࣛࣜࢆస〇ࡋࡓࠋᮏࣛ࢖ࣈࣛ
࣮ࣜࢆᶆ㆑ࡋࠊThermus thermophilus ⏝ DNA Chip㸦Affymetrix ♫〇㸧ࢆ⏝࠸ࡓ DNA ࣐࢖ࢡࣟ࢔ࣞ࢖࡟ࡼࡾࠊ඲㑇ఏᏊࡢ㌿෗㔞ࡢኚືࢆ⥙⨶ⓗ࡟ゎᯒࡋࡓࠋࡲࡓࠊࣉࣛ࢖࣐࣮
ఙ㛗ἲཬࡧ 5’-RACE ἲ࡟ࡼࡿ sip ㌿෗㛤ጞⅬࡢỴᐃ࡜ࠊࣀ࣮ࢨࣥࣁ࢖ࣈࣜࢲ࢖ࢮ࣮ࢩࣙ
ࣥཬࡧ LightCycler DX400㸦ࣟࢩ࣭ࣗ࢔ࣉࣛ࢖ࢻ࣭ࢧ࢖࢚ࣥࢫ♫〇㸧ࢆ⏝࠸ࡓ qRT-PCR
࡟ࡼࡿ sip ㌿෗ࡢᐃ㔞ゎᯒࢆ⾜ࡗࡓࠋ
2. 6. ⤌᥮࠼ࢱࣥࣃࢡ㉁࡟ࡼࡿࢩࣜ࢝ỿẊ ⤌᥮࠼ sip 㑇ఏᏊࢆ PCR ࡟࡚ቑᖜࡋࠊpET21a Ⓨ⌧࣋ࢡࢱ࣮࡟ᤄධࡋࡓࠋ⤌᥮࠼ࣉࣛࢫ࣑ࢻࢆ኱⭠⳦ Rosseta ᰴ࡟ᙧ㉁㌿᥮ࡋࠊ⤌᥮࠼యࢆస〇ࡋࡓࠋ⤌᥮࠼኱⭠⳦ࢆ LB ᇵᆅ࡟᥋✀ࡋࠊ๓ᇵ㣴⳦యࢆ᪂㩭࡞ LB ᇵᆅ࡟⛣᳜ࡋࠊIPTG ࡟ࡼࡿㄏᑟࢆ⾜ࡗ࡚⤌᥮࠼Sip ࢱࣥࣃࢡ㉁ࢆ⏕⏘ࡋࡓࠋࢱࣥࣃࢡ㉁⏕⏘኱⭠⳦ࢆ㉸㡢Ἴ◚○ࡋࠊ⇕ฎ⌮㸦70Υࠊ30 min㸧ᚋࠊࢽࢵࢣ࣮ࣝ࢟ࣞࢺ࣒࢝ࣛࢆ⏝࠸ࠊ⢭〇ࡋࡓࠋ⢭〇ࢱࣥࣃࢡ㉁ࢆࢩࣜ࢝⁐ᾮ࡟ῧຍࡋࠊ⤒᫬ⓗ࡟ᅇ཰ࡋࠊୖΎ࡟ྵࡲࢀࡿࢩࣜ࢝㔞ࢆ ICP Ⓨගศගศᯒ⿦⨨㸦࢚ࢫ࢔࢖࢔࢖࣭ࢼࣀࢸࢡࣀࣟࢪ࣮♫〇㸧࡟ࡼࡾᐃ㔞ศᯒࡋࡓࠋ
2. 7.

SEM ࠾ࡼࡧ XRD ゎᯒ ࢩࣜ࢝ࢫࢣ࣮ࣝ࠾ࡼࡧᚤ⏕≀ࡢᙧែࢆほᐹࡍࡿࡓࡵࠊ㉮ᰝᆺ㟁Ꮚ㢧ᚤ㙾㸦JEOL
JXA-733㸹᪥ᮏ㟁Ꮚᰴᘧ఍♫〇㸧ࣂ࢖࣑࢜ࢿࣛࣝࡢᙧែ࡜ࢩࣜࢥࣥࡢศᕸࢆ᳨ウࡍࡿࡓ㸫㸫ࡵࠊ㟁㟁Ꮚ⥺࣐࢖ࢡࣟ࢔ࢼࣛ࢖ࢨゎᯒࢆࢆ⾜ࡗࡓࠋEPMA ࡣ JEOL JCXAA-733㸦᪥ᮏᮏ㟁⾜ࡗࡓࠋᏊᰴᘧ఍♫〇㸧ࢆ⏝࠸࡚⾜⤖ᐹ
3. ⤖ᯝཬࡧ⪃ᐹ
 3. 1. ࢩࣜ࢝ࢫࢣ࣮ࣝᙧᙧᡂ࡟㛵୚ࡍࡍࡿ࠾ࡼࡧᛶ≧ゎゎᯒᚤ⏕≀ࡢ༢㞳࠾ ࢩ ࣜ࢝ࢫࢣ࣮࣮ࣝࡢᚤ⏕≀≀ྀࢆ㟁ᏊᏊ㢧ࡋࡓ࡜ࡇࢁࠊከᩘࡢࢩࣜ࢝ᚤ㙾࡟࡚ほᐹࡋࡓᱰ≧ࡢᚤ⏕⏕≀ࡀከᩘᩘほ⌫࡟ ໟᇙࡉࢀࡓ㸧ࠋḟ࠸࡛ࠊᆅ⇕Ⓨ㟁ᡤᡤ⁫ᐹࡉࢀࡓ㸦ᅗ 2㸧ᵴ࡟ᾐₕࡋࡓࡓ㖡ᯈ࡟ᙧᡂᡂࡋࡓࢩࣜ ࢝␃ᵴࢫࢣ࣮ࣝࢆ⤒᫬᫬ⓗ࡟ᅇ཰࣭ᦶ○ࡋࠊDNNAࢆᢳฟࡋࡓ࡜ࡇࢁࠊ୧⪅ࡢࡢ㛫࡟ࡣ᭷ពព࡞┦㛵㛵ಀࡀㄆࡵࡽࢀࡓ㸦ᅗ 3㸧
ࠋࡇࡢ DNNAᇦࢆ PCR ቑᖜࡋࠊቑሷᇶᇶ㓄ࡢ 166S rDNA 㡿ᇦิࢆゎゎᯒࡋࡓ࡜ࡇࢁࠊ㧗ᗘᗘዲ⇕⳦࡛࠶࠶ࡿࢢ࣒ࣛ㝜ᛶࡢ Thhermus ᒓ⣽⣽⳦ࡀ୺せ⳦⳦⩌ศ࠿ࡗࡓࠋ࡛࠶ࡿࡇ࡜ࡀศ ࡑࡇ࡛ࠊࢩࣜ࢝ࢫࢣ࣮ࣝࣝᦶ○≀ࢆ TMTኳᇵ ᆅ࡟ሬᕸᕸࡋ ࡚ 70 Υ࡛ᇵ㣴ࡋΥࡋࠊᐮኳ
Thermmus ᒓ⣽⳦ࡢศ㞳ࢆヨヨࡳࡓࠋࡑࡢ
ࡢ⤖ᯝࠊ㯤ᶳⰍࢆ࿊࿊ࡍࡿࢥࣟࢽࢽ࣮ࢆศ㞳ࡋࡋࠊY ᰴ࡜࿨ྡࡋࡋࡓ㸦ᅗ 4㸧ࠋᮏᰴࡣ⏕⌮⌮࣭
TMY⏕໬Ꮫヨ㦂ࡸ 166S rRNA 㑇ఏᏊሷᇶ㓄㑇㓄ิ࡞ ࡝ ࠿ ࡽ Theermus therm
mophilus TMMY(JCMM 10668)࡜ྠ
ྠᐃࡋࡓ 1)ࠋ

㸫㸫3. 2.

T. thermoophilus ࡟ࡼࡿࣂ࢖࢜ࢩࢩࣜ࢝ỿẊ ศ㞳ᰴ TMY ࢆ㐣㣬࿴ࢩࢆࣜ࢝ྵ᭷ TMM ᾮయᇵᆅᆅ࡛᣺࡜࠺ᇵᇵ㣴ࡍࡿ࡜ࠊᑐᩘቑṪᮇᮇ୰ᮇ௨㝆ࠊྵ᭷ࡉࢀ࡚࡚࠸ࡿࢩࣜ࢝࢝⃰ᗘࡀῶᑡᑡࡋࠊᑐᩘቑቑṪᮇᚋᮇ࡟࡟ῶᑡ㔞ࡀ᭱᭱኱࡟㐩ࡋࡓࡓ㸦ᅗ㸧ࠋࡇࡢ㝿ࠊࢩࣜ࢝ྵ᭷᭷ࡢ᭷↓࡟࠿࠿࠿ࢃࡽࡎࠊ⳦ࡢቑṪṪࡣኚ໬↓࠿࠿ࡗࡓࡓࡵࠊࢩࣜ
5(A)㸧
࢝ࡀ⳦ࡢቑṪ࡟࡟ᙳ㡪ࢆཬࡰࡰࡉ࡞࠸ࡇ࡜࡜ࡀ♧ࡉࢀࡓࡓࡀࠊᇵᆅ୰୰ࡢࢩࣜ࢝⃰⃰ᗘࡢῶᑡ࡟࡟క࠸ࠊࢤࣝ≧ࡢỿẊẊࡀ⏕ࡌࡓࠋࡇࡢỿẊ≀≀ࢆࣂ࢖࢜ࢩࢩࣜ࢝࡜࿨ྡྡࡋࠊࡑࡢᛶᛶ≧ࢆヨ㦂⟶ᗏ㒊࡟ࢤ
XRDD ࡟ࡼࡾゎᯒᯒࡋࡓ࡜ࡇࢁࢁࠊᆅ⇕Ⓨ㟁㟁ᡤ࡛ᙧᡂࡉࢀࡿࢩࣜ࢝ࢫࢣ࣮ࣝࣝ࡜ྠᵝࠊ㠀㠀⤖ᬗ㉁ࢩ࡛ࣜ࢝࠶ࡿࡇ࡜ࡀ♧ࡉࡉࢀࡓ㸦ᅗ 5(B)㸧
5ࠋࡇࡢࡇ࡜࠿ࡽࠊᆅ⇕Ⓨ㟁ᡤᡤ࡛ᙧᡂࡉࢀࢀࡿࢩ࡟ࡣࠊ㧗ᗘዲዲ⇕⳦ T. therrmophilus ࡀ㛵୚ࡍࡿࡇࡀࡇ࡜ࡀ♧၀ࡉࡉࢀࡓ 2)ࠋ
ࣜ࢝ࢫࢣ࣮ࣝ࡟
3. 3.

T. thermoophilus ࡢࢩࣜ࢝ㄏᑟᛶ
ᛶࢱࣥࣃࢡ㉁㉁㸦Silica-indduced proteinn; Sip㸧࠿ࡽࠊT. thermmophilus ࡀࢩࣜ࢝ࢫࢣ࣮ࣝᙧᡂ࡟࡟㛵୚ࡍࡿࡇࡇ࡜ࡀ᥎ᐹ࡛࡛ࡁࡓ ୖグࡢ⤖ᯝ࠿࠺࡟ヱ⳦ࡣࢩࢩࣜ࢝ࢆ⣽ࡀࠊᅗ 2 ࡢࡼ࠺ࡿࡢ࡛ࡣ࡞ࡃ
ࡃࠊ⣽⬊⾲⬊ෆ࡟྾཰ࡍࡿ┦஫స⏝ࡍࡿࡿ࡜⪃࠼ࡽᒙ࡛ࢩࣜ࢝࡜┦࡟ࡑࡢ㘽ࡀࢀࡓࠋࡑࡇ࡛ࠊ⣽⬊⾲ᒙ࡟࠶ࡿ࡜᥎࢝⃰ᗘࢆኚࡋࠊྵ᭷ࢩࣜ࢝ື ࡉ ࡏ ࡓ TM ᇵ ᆅ ࡛ ᇵ 㣴 ࡋ ࡓ T.
mophilus TMMY ᰴࡢ⣽⬊⬊⾲ᒙࢱࣥtherm
ࣃࢡ㉁ࢆᢳฟࡋ
ࡋࠊSDS-PAGGE ࡟౪ࡋࡽࢀࡿࡼ࠺
ࡓࠋࡑࡢ⤖ᯝࠊᅗ 6 ࡟ぢࡽ࡟ࠊ㐣㣬࿴ࢩࣜ࢝ྵ᭷ᇵᆅ
ᆅ࡛ᇵ㣴ࡋ㸫㸫ࡓ⳦య⾲ᒙࢱࣥ
ࣥࣃࢡ㉁࡟ࡢ
ࡢࡳࠊ᪂つࡢ
ࡢࢱࣥࣃࢡ㉁㉁ࡀ᳨ฟ࡛ࡁ
ࡁࡓࠋ ᮏࢱࣥࣃࢡ㉁㉁ࡢᛶ㉁ࢆ஧
஧ḟඖ㟁ẼὋ
Ὃື࡟᳨࡚ࡋ
ࡋࡓ࡜ࡇࢁࠊᮏࢱࣥࣃ
ࣃࢡ㉁ࡣศᏊ
Ꮚ㉁㔞
35kD
DaࠊpI9.5 ࡛ࠊࢩࣜ࢝↓࡛↓ῧຍ᫬࡟ẚẚࡋ࡚ 20 ಸ⛬ᗘࡢ⏕⏘ಸ⏘㔞ࡀㄆࡵࡽ
ࡽࢀࡓ㸦ᅗ 7㸧7 3)ࠋ ᮏ ࢱ ࣥ ࣃ ࢡ ㉁ ࡜ ྠ ᵝ ࡢ ࢱ ࣥ ࣃ ࢡ ㉁ ࡣ ࠊ TMYY ௨ እ ࡢ Thermus ᒓ ⣽ ⳦ ࡛ ࠶ ࡿ T.
therm
mophilus HB8ࠊT. thermoophilus AT-662ࠊT. aquatiicus YT-1 ࡞࡝࡛ࡶ᳨ฟ࡞
ฟࡉࢀࠊ㐣㣬㣬࿴ࢩࣜ࢝࡟ᛂ⟅ࡍࡿ Thermus ᒓ⣽⳦࡟≉᭷ᒓ᭷࡞ࢱࣥࣃࢡ
ࢡ㉁࡛࠶ࡿ࡜
࡜᥎ࡋࡓࠋࡑࡇ࡛ࠊࡇࡇࢀࡽࡼࡗ࡚⏕⏘ㄏㄏᑟࡉࢀࡿ࠿࠿ྰ࠿ࢆ᳨ウウࡋࡓ㸦ᅗ 88㸧ࠋࢱࣥࣃࢡ㉁ࡀࠊࢩࣜ࢝࡟ࡼ

㸫㸫 ࡑࡢ⤖ᯝࠊࢱࣥࣃࢡ㉁ࡣࢩࣜ࢝ῧຍ
ຍᚋ 1 ᫬㛫௨௨ෆ࡟⏕⏘ࡀࡀ㛤ጞࡉࢀࠊ↓ῧຍࡢሙሙྜࡣ⏕⏘ ࡉࢀ࡞࠿ࡗࡗࡓࠋᚑࡗ࡚
࡚ࠊᮏࢱࣥࣃ
ࣃࢡ㉁ࢆࢩࣜ࢝ㄏᑟᛶ
ᛶࢱࣥࣃࢡ㉁㉁㸦Silica-induced
protein; Sip㸧࡜࿨࿨ྡࡋࡓࠋࢩ
ࢩࣜ࢝࡟ࡼࡗ࡚⏕⏘ࡀㄏᑟࡉࢀࡿ
ࡿࢱࣥࣃࢡ㉁㉁ࡣࠊୡ⏺ึึࡢⓎぢ࡛࠶ࡗࡓ 3)ࠋ
࣮ࢻࡍࡿ㑇ఏ
ఏᏊࡢྠᐃ࡜
࡜ゎᯒ
3. 4. Sip ࢆࢥ࣮ࡍࡿ㑇ఏᏊࢆࢆ᥈⣴ࡍࡿࡓ
ࡓࡵࠊTMY ᰴ࠿ࡽᮏࢱࣥࣃࢡ㉁ࢆ
ࢆศ㞳࣭⢭〇ࡋࡓࠋ Sipp ࢆࢥ࣮ࢻࡍ࡜ࡇࢁࡀࠊ⢭〇〇ࢱࣥࣃࢡ㉁㉁ࡢ࢔࣑ࣀᮎ
ᮎ➃ࡣࣈࣟࢵࢵࢡࡉࢀ࡚࠾࠾ࡾࠊᖖἲ࡛
࡛࠶ࡿ N ᮎ➃ゎᮎศゎ㓝⣲࡟ࡼࡿศゎ࡛⏕⏕ࡌࡓ᩿∦ࢆࢆ⏝࠸ᯒࡣ୙ྍ⬟࡛࠶ࡗࡓࠋࡑࡇ࡛ࠊྛ✀ࢱࣥࣃࢡ㉁ศ
㓟㓄ิࢆỴᐃᐃࡋࡓࠋࢱࣥ
ࣥࣃࢡ㉁ศゎゎ㓝⣲ Asp-NN ࢆ⏝࠸࡚࡚⏕ࡌࡓ᩿∦∦ࡢ࢔ࡓෆ㒊࢔࣑ࣀ㓟Ỵᐃࡋࠊ᪤࡟ࢤࣀ࣒㓄ิิࡢỴᐃࡉࢀࢀ࡚࠸ࡿ HB88 ᰴ࡜ࡢ┦ྠྠᛶ᳨⣴ࡢ⤖⤖ᯝࠊ࣑ࣀ㓟㓄ิࢆỴᛶ ABC ࢺࣛࣛࣥࢫ࣏࣮ࢱࢱ࣮࡜᭷ព࡞࡞┦ྠᛶࢆ♧♧ࡋࡓ㸦ᅗ 9㸧9 3)ࠋ
ᮏࢱࣥࣃࢡ㉁ࡣࠊFe3+⤖ྜᛶ ᮏ㑇ఏᏊࡣࠊ㕲㕲㣚㣹᮲௳ୗ࡛ࡢ 3 ౯ࡢ㕲࢖࢜ࣥ౯ࣥྲྀ㎸ࡳ࡟ᶵ
ᶵ⬟ࡍࡿࡇ࡜࡜ࡀ᥎ᐹࡉࢀࢀࠊ࠸⳦ࡀឤᰁࡲࡓ
ࡓࡣ⑓ཎᛶࢆ
ࢆⓎ㟢ࡍࡿ㝿㝿࡟ᚲせ࡞㕲
㕲࢖࢜ࣥࢆࢆ⣽⬊ෆ࡟ྲྀྲྀࡾ㎸ࡃࡘ࠿ࡢ⑓ཎ⳦
ࡴࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋ࡜ࡇࢁࡀࠊᮏ⳦ᰴࡣ⑓⑓ཎᛶ⳦࡛ࡣࡣ࡞ࡃࠊࡲࡓࡓࠊ㕲࢖࢜ࣥࣥ࡜ࢩࣜ࢝࡜࡛ࡣࠊࡑࡢ㟁Ⲵࡶᶵᶵ⬟ࢆ኱ࡁࡃ␗࡞ࡿࠋࡋࡋࡓࡀࡗ࡚ࠊᮏ㑇ఏᏊࡀࡀ࡞ࡐࠊ㐣㣬㣬࿴ࢩ
ࡳⓎ⌧ࡍࡿ࠿
࠿ࡣ୙࡛᫂࠶
࠶ࡗࡓ㸦ᅗ 10㸧
1 ࠋࣜ࢝ῧຍ᫬ࡢࡳ

㸫㸫3. 5. sip 㑇ఏᏊᏊࡢ㌿෗ゎᯒᯒ ୖ グࡢ␲ၥࢆ
ࢆゎỴࡋࠊࢩ
ࢩࣜ࢝ࢫࢣ ࣮ࣝᙧᡂࡢࡢᘬࡁ㔠ࢆ᥈᥈⣴ࡍࡿࡓ ࡵࠊࢩࣜ࢝
࢝⃰ᗘ
㸦200ppmࠊ400pppmࠊ600pppm㸧ࢆኚ໬ࡉࡏࡓࠊࡲࡓࡣ࣮࣮࢟ࣞࢺᶞ⬡࡟ࡼࡼࡾ㕲࢖࢜ࣥ
ࣥࢆ㝖ᆅ୰࡛ᇵ㣴ࡋ
ࡋࡓ T. thermoophilus HB8
8 ᰴ࠿ࡽ RNNA ࢆศ㞳࣭⢭〇ࡋࠊࡇࡇࢀࡽཤࡋࡓᾮయᇵᆅࢆ㗪ᆺᆺ࡜ࡋࡓ RT-PCRRTἲ࡟ࡼࡗ࡚ྛ㑇㑇ఏᏊࡢ㌿෗෗㔞ࢆᐃ㔞ゎゎᯒࡋࡓ㸦ᅗ
ᅗ 11㸧ࠋ ࡑࡢ⤖ᯝࠊTTTHA1628㸦ssip㸧ࡢࡳࡀࠊ㐣㣬࿴ࢩࢩࣜ࢝࡟ᛂ⟅⟅ࡋ࡚㌿෗㔞㔞ࡀቑ኱ࡋࡓࡓࠋࡲᮏ㑇ఏᏊࡣ㣬㣬࿴௨ୗࡢ⃰ᗘ࡛ࡣࠊ㌿෗㔞ࡣ 2 ಸ⛬ᗘ࡛ࡋࡋ࠿↓ࡃࠊ㕲㕲㣚㣹≧ែ࡛࡛ࡣ㌿ࡓࠊᮏ෗㔞ࡣ 10 ಸ௨ୖୖ࡟ቑຍࡋࡓࡓ 4)ࠋࡇࢀࡽ
ࡽࡢ⤖ᯝࡣࠊDNA ࣐࢖ࢡࣟ࢔ࣞ࢖࢖ࡢ⤖ᯝ࡜ࡶࡶྜ⮴㣹ࡲࡓࡣ㐣㣬㣬࿴ࢩ่⃭ࣜ࢝࡟ᛂ⟅⟅ࡋ࡚Ⓨ⌧ࡉࡉࢀࡿࢱࣥࣃ
ࣃࢡ㉁ࡋࡓࡇ࡜࠿ࡽࠊsip ࡣ㕲㣚㣹᥎ᐹࡉࢀࡓࠋ࡛࠶ࡿࡇ࡜ࡀ᥎ࡅࡿࢩࣜ࢝ࢫࢣ࣮ࣝᙧ
ᒓᙧᡂࡢ࣓ࣜࢵ
ࢵࢺ
3. 6. Thermus ᒓ⣽⳦࡟࠾⣽⳦࡟࡜ࡗ࡚
࡚ Sip ࢱࣥࣃࢡ㉁ࢆ⏕⏘⏘ࡋ࡚ࢩࣜ࢝ࢫࢣ࣮ࣝࢆ
ࢆᙧᡂࡍࡿ࣓
࣓ࣜࢵ Thermus ᒓ⣽ࡲࡎࡣࠊ⮬↛↛ࡢᆅ⇕⎔ቃቃ୰࡟ぢࡽࢀࡿࢩࣜ࢝ỿẊ࡛࠶ࡿࡿࢩࣥࢱ࣮ࢆࢆほᐹࢺࢆ⪃ᐹࡋࡓࠋࡲ‪ฟᏍ㏆㎶࡟࡟ᙧᡂࡉࢀ࡚࡚࠸ࡿࠋࡲࡓ
ࡓࠊThermus ᒓ࡞࡝ࡢ㧗㧗ᗘዲ⇕⳦ࡢࡢ⏕⫱ࡍࡿ࡜ࠊ⇕Ỉ‪࡜ᖜᗈࡃ࡞࠸࠸ࡇ࡜࠿ࡽࠊヱ⳦ࡣ⮬㌟ࡢ⏕⫱࡟࡟㐺ࡍࡿ
ᗘࡣ 70 ๓ᚋ࡜㊊ሙ࡜ࡋ࡚฼฼⏝ࡋ࡚࠸ࡿࡿࡇ࡜ࡀ⪃࠼࠼ࡽࢀࡿࠋࡵࠊࢩࣜ࢝ࢆ㊊㸫㸫ᗘᗘᇦ࡟࡜࡝ࡲࡲࡿࡓ ࡲࡓࠊࢩࣜ࢝ࡀᢠ⏕≀㉁㉁࡟ᑐࡍࡿ⪏⪏ᛶࢆྥୖࡉࡉࡏ࡚࠸ࡿࡇࡇ࡜ࡶุࡗࡓࡓ 5)ࠋ࢔ࣥࣆ
ࣆࢩ࣮࣒ࣜ࡟స⏝ࡋ࡚ࢱࣥࣃࢡ㉁ྜᡂࢆ㜼ᐖࡍࡿ⸆⸆๣ࢆࢩࣜ࢝
࢝ῧຍࡋࡓ TMT ᾮࣥ࡞࡝ࠊࣜ࣎ࢯ࣮
ࡋ࡚ࠊT. thermmophilus HBB8 ࢆᇵ㣴ࡋ࡚ࡶࠊࢩࣜ࢝↓ῧຍ᫬᫬࡜ྠᵝࠊቑቑṪࡀయᇵᆅ࡟ῧຍࡋ
ࡢࡢ㸦ᅗ 12((A)㸧ࠊ⣽⬊⭷⭷ᙧᡂ㜼ᐖ࡟㛵୚ࡍࡿࣂࢩࢺࣛࢩࢩࣥࡸࢥࣜࢫ
ࢫࢳࣥ㜼ᐖࡉࢀࡿࡶࡢࡣࠊࢩࣜ࢝ῧῧຍ᫬࡟ቑຍ
ຍࡋࡓ㸦ᅗ 12(B)㸧ࠋࡉࡽ
ࡽ࡟ࠊT. therrmophilus HBB8 ࢤ࡟ᑐࡍࡿ⪏ᛶࡣ
᤼ฟ࣏ࣥࣉ㑇
㑇ఏᏊࡢⓎ⌧⌧ࢆ RT-PCRR ࡟᳨࡚ウࡋࡋࡓࡀࠊ඲࡚
࡚ࡢ㑇ࣀ࣒ୖ࡟᥎ᐃࡉࢀࡿ⸆๣᤼
࠾ࡼࡧ⸆๣ࡢ
ࡢ᭷↓࡟࠿࠿ࢃࡽࡎ㌿㌿෗㔞ࡢ᭷ពព࡞ቑῶࡣ☜☜ㄆ࡛ࡁ࡞࠿࠿ࡗࡓఏᏊࡀࠊࢩࣜ࢝࠾⏕⌮ࠊ≉࡟ࢱࣥࣃࢡ㉁⏕⏕ྜᡂࢆಖㆤㆤࡍࡿ㸦ᅗ 13㸧ࠋࡇࢀࡽࡢ⤖ᯝ࠿ࡽࠊࢩࣜ࢝ࡣᮏ⳦ࡢ⏕࠶ࡓ࠿ࡶࠕ㙚㙚ࠖࡢࡼ࠺࡟࡟㔜࡞ࡾࠊ⣽⣽⬊⾲ᒙࠊ≉≉࡟⭷ࡢሀ∼∼ᛶࢆࡢ࡛ࡣ࡞ࡃࠊ⣽⬊⾲ᒙ࡟࠶᥎ᐹ࡛ࡁࡿࠋ㧗ࡵ࡚࠸ࡿ࡜᥎㸫㸫㸫㸫3. 7. Sip ࢆ⏝࠸࠸ࡓࣂ࢖࣮࢜ࣜࣜࢳࣥࢢ ௨ୖࡢ⤖ᯝࢆ⧳ࡵࡿ࡜ࠊSip ࡣ㐣㣬࿴࿴ࢩ่่⃭ࣜ࢝ࡲࡓࡣࠊ㈇࡟ᖏ㟁ࡋࡋࡓ㐣㣬࿴ࢩࢩࣜ࢝࡜ṇ㟁Ⲵࢆᖏࡧࡧࡓ㕲࢖࢜ࣥࣥࡀ⤖ྜࡍࡿࡿࡇ࡜࡟ࡼࡾࡾࠊ౑⏝࡛ࡁࡿ㕲࢖࢜ࣥࣥࡀᯤῬࡍࡿࡿࡇ࡜࡛㕲㣚㣹≧ែ࡟࡟ᛂ⟅ࡋ࡚ㄏㄏᑟⓎ⌧ࡋࠊ⣽⬊⾲ᒙ࡟࡟ⴭ㔞⏕⏘ࡉࡉࢀࠊࡇࡢࡼ࠺࡞≧ែ࡟࡟࡞ࡗࡓ⣽⬊⬊ࡀࢩࣜ࢝࡜┦஫స⏝ࡋࡋ࡚ࢩࣜ࢝ࢫࢫࢣ࣮ࣝࢆᙧᡂࡍࡿࡇ࡜ࡀ᥎ᐹࡉࢀࢀࡓࠋࡋࡓࡀࡗ࡚ࠊᮏࢱࣥࣃࢡ㉁ࡀ
ࡀ⣽⬊⾲ᒙ࡛ࢩࣜ࢝࡜⤖⤖ྜࡍࡿࡢ࡛࡛ࡣ࡞࠸࠿࡜᥎᥎ᐹࡋࡓࠋ ࡑࡇ࡛ࠊྠᐃ
ᐃࡋࡓ sip 㑇ఏᏊ⏘㑇࡛኱㔞Ⓨ⌧ࡉࡉࡏࠊᚓ≀ࢆ኱⭠⳦ෆ࡛ࡽࢀࡓ⤌᥮࠼ Sip ࢆ⏝࠸࡚࡚ࣂ࢖࢜ヨࡳࡓ㸦ᅗ 144㸧6)ࠋ
࣮ࣜࢳࣥࢢࢆヨ᥮࠼ Sip ࢆ⢭⢭〇ࡋ㐣 ⏕⏘ࡋࡓ⤌᥮ᾮ࡟ΰ࿴ࡋࡓࡓ࡜ࡇࢁࠊ㣬࿴ࢩࣜ࢝⁐ᾮࡋࡓሙྜࡢࡳ
ࡳࠊࢩࣜ
70Υ࡟࡚ຍ⇕ࡋẊࡀ᳨ฟ࡛࡛ࡁࡓ㸦ᅗ 155㸧ࠋ࢝ỿẊ ࡇࡢỿẊࢆヲヲ⣽࡟ศᯒࡍࡍࡿ࡜ࠊᚤ⣽⣽࡞⌫ࡀከᩘᩘᙧᡂࡉࢀ࡚࡚࠾ࡾࠊࡇࡢࡢ⌫ࡣࢩࣜ࢝࢝ࢆ㧗㸫㸫⃰ᗘྵ᭷ࡋ࡚࠸ࡿࡇ࡜ࡀุࡗࡓࠋࡋࡓࡀࡗ࡚ࠊSip ࡀࢩࣜ࢝࡟┤᥋⤖ྜࡋࠊ⣽⬊⾲ᒙ࡟ࢩࣜ࢝ࢆỿ╔ࡋ࡚࠸ࡿࡇ࡜ࡀ⪃࠼ࡽࢀࡓࠋ ࡇࡢ⤖ᯝ࠿ࡽࠊᮏ Sip ࢱࣥࣃࢡ㉁ࢆ฼⏝ࡍࡿࡇ࡜࡛ࠊ௵ព࡟ࢩࣜ࢝ࢆỿẊࡉࡏࡿࡇ࡜ࡀྍ⬟࡜࡞ࡿࠋࡇࢀࡣࠊࢩࣜ࢝ࡢࣂ࢖࣮࢜ࣜࢳࣥࢢࡔࡅ࡛࡞ࡃࠊᚤ⣽ᵓ㐀ᙧᡂࡀᚲせ࡜࡞ࡿࢼࣀࢸࢡࣀࣟࢪ࣮࡬ࡢᛂ⏝ᒎ㛤ࡶྍ⬟࡛࠶ࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿ 7)ࠋ௒ᚋࠊࡉࡽ࡟ຠ
⋡ⓗ࠿ࡘྍረᛶࡢ࠶ࡿࢩࣜ࢝ỿẊࢆ┠ᣦࡋࡓ࠸ࠋ≉࡟ Sip ࡢ❧యᵓ㐀ゎᯒ࠿ࡽࠊࢱࣥࣃࢡ㉁࡜ࢩࣜ࢝࡜ࡢ⤖ྜ㒊఩ࢆྠᐃࡋࠊᮏ㒊఩ࢆ༢㞳ࡲࡓࡣಟ㣭ࡋ࡚ࠊࡉࡽ࡟㧗ᶵ⬟࡞Sip ࡢస〇ࢆヨࡳࡓ࠸࡜⪃࠼࡚࠸ࡿࠋࡇࢀࡽࢆ฼⏝ࡍࡿࡇ࡜࡛ᆅ⇕⇕Ỉ࠿ࡽࢩࣜ࢝ࡢࡳࢆ㝖ཤࡲࡓࡣ༢㞳ࡋࠊ㧗⣧ᗘỈ࢞ࣛࢫ࡞࡝ࡢㄪ〇ࡸࠊࢩࣜ࢝㝖ཤ⇕Ỉ࠿ࡽࡢࣜࢳ࣒࢘ࡢᅇ཰࡟⧅ࡆ࡚࠸ࡁࡓ࠸࡜⪃࠼࡚࠸ࡿࠋ

4. ㅰ㎡ ᮏ◊✲ࡣࠊJFE 21 ୡ⣖㈈ᅋ 2009 ᖺᗘ ᢏ⾡◊✲ຓᡂ࡟ࡼࡾᐇ᪋ࡉࢀࡓࡶࡢ࡛ࠊJFE 21ୡ⣖㈈ᅋ㛵ಀྛ఩࡟῝⏒࡞ࡿឤㅰࢆ⏦ࡋୖࡆࡲࡍࠋࡲࡓࠊඹྠ◊✲⪅࡛࠶ࡿ஑ᕞ኱Ꮫ኱Ꮫ㝔⌮Ꮫ◊✲㝔ᩍᤵᶓᒣᣅྐඛ⏕ࡢࡈᑾຊ࡟ㅰពࢆ⏦ࡋୖࡆࡲࡍࠋᮏ◊✲ࡢ୍㒊ࡣࠊ஑ᕞ኱Ꮫ኱Ꮫ㝔⏕≀㈨※⎔ቃ⛉Ꮫᗓ༤ኈᚋᮇㄢ⛬ ⸨㔝ὈᐶẶ㸦⌧ ஑ᕞ኱Ꮫ㧗➼ᩍ⫱㛤Ⓨ᥎㐍ࢭࣥࢱ࣮ຓᩍ㸧ࡢ༤ኈㄽᩥ◊✲ࡢෆᐜࢆྵࢇ࡛࠾ࡾࠊ⸨㔝༤ኈ࡟ࡶᚰ࠿ࡽឤㅰ⏦ࡋୖࡆࡲࡍࠋ

5. ཧ⪃ᩥ⊩1) Y. Fujino, R. Kawatsu, F. Inagaki, A. Umeda, T. Yokoyama, Y. Okaue, S. Iwai, S. Ogata, T.
Ohshima and K. Doi, Thermus thermophilus TMY isolated from silica scale taken from ageothermal power plant, J. Appl. Microbiol., 104, 70-78 (2008).
2) Doi, K., Fujino, Y., Ohshima, T. and Yokoyama, T.; Characterization of a Silica-InducedProtein in Thermus Thermophilus Related to Biosilicification, Goldschmidt Conference
Abstracts, A238 (2010).
3) K. Doi, Y. Fujino, F. Inagaki, R. Kawatsu, M. Tahara, T. Ohshima, Y. Okaue, T. Yokoyama, S.
Iwai and S. Ogata; Stimulation of Expression of a Silica-Induced Protein (Sip) in Thermus
thermophilus by Supersaturated Silicic Acid, Appl. Environ. Microbiol., 75, 2406-2413(2009).
4) Fujino, Y., Ohshima, T., Yokoyama, T and Doi, K.; Transcriptional Analysis of the Responseto Supersaturated Silicic Acid in Thermus Thermophilus, Goldschmidt Conference Abstracts,A309 (2010).

㸫㸫5) S. Iwai, K. Doi Y. Fujino, T. Nakazono, K. Fukuda, Y. Motomura and S. Ogata, Silica
deposition and phenotypic changes to Thermus thermophilus cultivated in the presence ofsupersaturated silica, ISME J., 4, 809–816 (2010).
6) M. Farahat, T. Hirajima, K. Sasaki, K. Doi; Adhesion of Escherichia coli onto quartz,
hematite and corundum: Extended DLVO theory and flotation behavior, Colloid. Surface B,
74, 140-149 (2009)7) ᅵᒃඞᐇ㸸ࠕ㧗ᗘዲ⇕ᛶ⣽⳦ࢆ⏝࠸ࡓࣂ࢖࣮࢜ࣜࢳࣥࢢࡢᇶ♏࡜ᛂ⏝ࠖࠊྜྷ⏣࿴ဢࠊụ㐨ᙪࠊ᳜⏣඘⨾┘ಟ࣓ࠗࢱࣝࣂ࢖࢜ࢸࢡࣀࣟࢪ࣮࡟ࡼࡿ⎔ቃಖ඲࡜㈨※ᅇ཰࠘ࠊp. 115-120㸦2009㸧ࠊࢩ࣮࢚࣒ࢩ࣮ฟ∧㸫㸫