The Platform for Therapeutic Oligonucleotides

AnalyzeandQuantify
Nucleic Acids“AQXeNA” is a powerful analysis platform for automated identification andquantification of XNA* from the large amounts of mass spectrometric dataobtained from LC-MS experiments. AQXeNA can provide the most accurate andconfident characterization of XNA more quickly than ever before, achieving
beneficial results in Chemical Manufacturing Control and Pharmacokinetics ofOligonucleotide therapeutics.
*XNA: Entire range of nucleic acids including natural DNA/RNA, synthetic nucleic acids and analogues.

Product features
Raw data from LC-MS or
LC-MS/MS

Identification andanalysis of XNA

Various sophisticated algorithm and interfaces are implemented for analysis of
XNA using mass spectrometry.

Definition of
XNA structures

Easily customizable
XNA structure
database

Registration oftarget XNA
sequence

User-friendly sequence
editor enables users todefine XNA sequences

XNA IdentificationandQuantification

High-throughput
peak detection anddeconvolution
algorithms

Analysis andreporting

Effective
reporting of XNA
results

Enabled devices and data formats• Thermo Fisher Scientific• Waters (.RAW)• Sciex (.WIFF)• Agilent (.D)
• Bruker (.D)
• Shimadzu (.LCD)• MzML (.mzML)(.RAW)

Ariadne: Algorithm for
Accurate XNA ID fromMS2 data

Please contact us for the latest information.

Fig. the workflow of AQXeNA

Analyze Identification
and Quantify
Xeno
Automated
SystemForNucleic
Lipidome Acids

AQXeNA includes two different technology• Ariadne: Accurate XNA Identification algorithm, which is the first in the world.
• Ion peak detection algorithm: We have accumulated our knowledge of ion peakdetection by development of LipidSearch*.
*LipidSearch: analysis software for lipidomics, which is used in more than 300 institutes of 30 countries.

Easily customizable XNA structure database
XNA structures such as nucleosides, linkers, 5′/3′ ends, modificationgroups, and MS/MS dissociation profiles generated not only by
CID/HCD but also from UVPD can easily be defined in the database.

User-friendly sequence editor✓✓

can define any nucleic acid sequence includinghydrosylates by sequence-specific endonucleases orexonucleases.
makes it easy to perform the characterization of longmRNAs for vaccines as well as metabolic analyses ofantisense and siRNA drugs.

High-throughput peak detection and deconvolution algorithm
AQXeNA provides high accuracy peak detection of charge state,monoisotopic m/z, and peak area using a sophisticated algorithm
based on a Gaussian fitting method from large data sets.

Ariadne: Accurate XNA identification algorithm✓✓

Ariadne, which has been developed by Dr. Nakayama of
RIKEN achieves more accurate identification of nucleic
acids using MS2 spectra.
It is patented in Japan and the US.

Effective reporting of results
AQXeNA supports reporting results such as
multivalent ion deconvolution and comparison
between Treatment and Control groups.

【 Contact 】※ Ariadne：Database search for RNA identification using tandem mass spectrometry data
（https://ariadne.riken.jp/）

Bioscience Div., Solution Knowledge Center
Atago Green Hills MORI Tower
2-5-1 Atago, Minato-ku, Tokyo, 105-6215 Japan
Tel +81-3-6376-1291 FAX +81-3-3435 -0522
E-Mail ： bio-psg-pro-dg@mki.co.jp
Corporate website : https://www.mki.co.jp/english/