YMC USER'S MANUAL

Column Care and Use Instructions

Alcyon SFC Triart C18, Triart Diol, Triart PFP, CN, SIL
Supercritical Fluid Chromatography Column
1. Introduction
Thank you for purchasing an Alcyon SFC Column for Supercritical Fluid Chromatography (SFC).
Alcyon SFC Columns, which are manufactured under highly controlled conditions, must pass a series of strict tests before being
accepted for shipment (Please refer to the column inspection report). To ensure optimal performance and durability of the column,
please read these instructions carefully before using this column.

2. SpecificationsPackingmaterialParticlesize(µm)

Poresize(nm)Functional group

Triart C18C18

Triart Diol

Dihydroxypropyl

Triart PFP

512End capped

Usabletemperature range
Regular use(recommended)

Upper limit

20 – 40 ℃

50 ℃

Yes

Pentafluorophenyl

No

CN

Cyanopropyl

Yes

SIL－－

3. Precautions for use
・ The column endfitting is Waters style connection.
・ Tubing must have flat ends and must bottom out in the column endfitting. Tubing must be connected to the column correctly toavoid creating a void between the column frit and tubing, which can cause a leak and result in poor column performance (e.g.
peak tailing, loss of theoretical plate number).
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ Do not disconnect a column from the SFC system before the pressure drops to zero.
・ The column pressure limit and recommended flow rate are the following.
Particlesize

Pressure limit

5 µm

Column length of 150 mm : 20 MPa
Column length of 250 mm : 25 MPa
Inner diameter of 10 mm and larger : 10 MPa

Column I.D. and recommended flow rate
2.1 mmI.D.
4.6 mmI.D.
10 mmI.D.
20 mmI.D.

: 0.2 – 0.6 mL/min (Max. flow rate : 1.0 mL/min): 1.0 – 3.0 mL/min (Max. flow rate : 5.0 mL/min): 5 – 15 mL/min (Max. flow rate : 25 mL/min): 20 – 60 mL/min (Max. flow rate : 100 mL/min)・ Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
・ Recommendations of temperature for column use are shown in the specifications table in section 2.
・ In general, usage at higher temperatures, higher concentrations of additives can shorten the column lifetime.

4. Shipping solvent
100% 2-propanol. Replace with this solvent for storage.

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YMC USER'S MANUAL

5. Mobile phase and sample solvent
・ When a target compound is ionic, addition of additives listed below can improve peak shape and/or separation reproducibility.
High concentrations of additives can result in reducing column lifetime. Add/reduce the additives according to the notes in thetable. Additive concentration below is concentration for the entire mobile phase.
・ When possible, the sample should be dissolved in the same modifier as the mobile phase. Using a stronger solvent than mobile
phase for sample dissolution might result in distorted peak symmetry and degraded resolution.
・ In order to avoid blockage which can cause pressure increase, the sample solution should be filtered through a membrane filter(0.2 μm or smaller porosity).
【Recommended solvents】
Acidic compounds
Modifiers
Additives

Basic compounds

alcohols (methanol, ethanol, 2-propanol), acetonitrile, ethyl acetate,tetrahydrofuran (THF), dichloromethane, chloroform, methyl tert-butyl ether (MTBE), etc
0.1% (Upper limit 0.5%)
trifluoroacetic acid (TFA),
acetic acid, formic acid, etc

0.1% (Upper limit 0.5%)
diethylamine (DEA), butylamine,ethanolamine, etc

Composition ratio

6.

Non-Ionic compounds

None

CO2/modifiers (99/1 – 40/60)

Column cleaning (general method)・ Flush the column with solution containing a higher ratio of modifiers (for example, for CO2/methanol mobile phase,
concentration of methanol should be increased) for washing out the compounds that have a great capacity for retention in thecolumn. When further cleaning is required, flush with 100% ethanol is effective.
・ When a mobile phase containing acid or amine is used, replace with CO2/modifiers containing neither of them (at the sameratio as the mobile phase), then wash as above procedure. Storing a column with a mobile phase containing additive is notrecommended even for a short period of time.
・ The column needs to be replaced when these cleaning methods do not regenerate the column performance. To extend thecolumn lifetime, especially for samples containing large amount of impurities, we recommend a sample pretreatment conductedcarefully prior to introducing the sample to the column.

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Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp
E-Mail:sales@ymc.co.jp(UM150707AE) 2/2