YMC USER'S MANUAL

Column Care and Use Instructions

YMC-Pack Diol
1. Introduction
Thank you for purchasing a YMC high-performance liquid chromatography (HPLC) column. “YMC-Pack Diol” is a HPLC column forgel filtration chromatography column that the diol group was chemically bonded to the porous spherical silica gel. The functional group ischemically bonded with low nonspecific adsorption even in the separation of hydrophilic proteins.
YMC HPLC columns, which are manufactured under highly controlled conditions, must pass a series of stringent tests before being
accepted for shipment. (Please refer to the column inspection report). To ensure optimal performance and durability of the column,
please read these instructions carefully before using this column.

2. Specifications
ColumnParticlesize(µm)

Diol-60

Pore size(nm)

Functional group

UsablepH range

Temperature(Upper limit)

Dihydroxypropyl

5.0 – 7.5

40 °C

63, 5Diol-120

12Diol-200

202, 3, 5Diol-300

30

3. Recommendations for column connections
・ The "PTH" or "WT" at the end of the product code indicates the style of column endfittings (see below for details).

Consideration of connector and endfittings
Tubing and connector

ColumnPort depth

Particle size

The end of theproduct code

Port depth

Style of endfittings

2 µm

PTH

ca. 2 mm / 0.09 inch

Parker style

3 µm, 5 µm

WT

ca. 3 mm / 0.13 inch

Waters style・ Tubing must have flat ends and must bottom out in the column endfitting. Tubing must be connected to the column correctly to
avoid creating a void between the column frit and tubing, which can cause a leak and result in poor column performance (e.g.
peak tailing, loss of theoretical plate number).

4. Shipping solvent
The column is shipped in the 0.05% sodium azide aqueous solution. Flush with water sufficiently before replacing with the mobilephase.

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YMC USER'S MANUAL

5. Precautions for use
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ The column pressure limit and recommended flow rate are the following.
Particle size

The end of theproduct code

2 µm

PTH

3 µm, 5 µm

WT*1Pressure limit*245 MPa

30 MPa or less for regular use*220 MPa*1 Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
*2 Adjust the flow rate appropriately because the pressure changes depending on the column length, temperature, types of mobile phaseetc.

・ Aqueous mobile phase are basically used. Total salt concentration of mobile phase should be lesser than 0.7 M. Tris-HCl, citrate, etc.
are applicable as buffer solution. These buffer solutions are available with solutions containing buffer salt/additives such as sodium
chloride, sodium sulfate, and ammonium sulfate.
・ Aqueous solutions of urea and guanidine hydrochloride which are used for a denaturant of the protein can be used. Moreover, 0.1%
or less concentration of surface-active agents such as Tween80, SDS is also usable. When using these mobile phases, theequilibration of the column needs long time as compared to the general mobile phase.
・ Methanol or acetonitrile can be added to mobile phase. When using a mobile phase containing methanol or acetonitrile, be
mindful of the operation pressure rising by increasing viscosity and the precipitation of buffer salts/additives.
・ Recommendations of pH and temperature for column use are shown in the specifications table in section 2.
・ Column lifetime varies depending on conditions of use such as pH, temperature and mobile phase composition. In general, usage at
higher temperatures and higher concentrations of buffer salts/additives can shorten the column lifetime.
・ For storage except daily use, the column should be flushed thoroughly with water, replaced in aqueous solution of 0.05% of sodium
azide, which should be sealed the both ends tightly and stored in a location with minimal temperature change. In next time, flush withwater sufficiency before replacing with the mobile phase.

6. Column cleaning (general method)
In the case that some hydrophobic proteins or the hydrophobic materials are adsorbed or retained, flush the column with solvent
containing high salt concentration (approx. 0.5 M). At that time, be careful about usable pH.

7. Other environments
・ The operating pressure should be kept under 5 MPa (725 psi) for Glass column.
・ To prevent exposure of the column to excessive pressure, the sample solution should be filtered through a 0.2 μm membrane orsmaller to remove particulates. We recommend using a pre-column filter to prevent the column frit from being clogged with samples.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM170306AE) 2/2