YMC USER'S MANUAL

Column Care and Use Instructions

YMC-Actus Series
1. Introduction
Thank you for purchasing a YMC-Actus series column. YMC-Actus series is designed for preparative HPLC with excellent
durability even under ballistic gradient conditions causing large pressure fluctuation.
YMC-Actus series, which are manufactured under highly controlled conditions, must pass a series of stringent tests before being
accepted for shipment. (Please refer to the column inspection report). To ensure optimal performance and durability of the column,
please read these instructions carefully before using this column.

2. Specifications
Particlesize (µm)

Poresize (nm)C%

Triart C18

51220

Triart C18 ExRS

5825

Triart C851217

Triart Phenyl

51217

1–10

Triart PFP

51215

1–8

Triart Bio C18

530－

1–12

Triart Bio C4

530

－

1–10

Pro C18

51216

Hydrosphere C18

51212

Pro C18 RS

5822

1–10

Pro C8

51210

2–7.5

ODS-A

51217

ODS-AQ

51214

Packing material

UsablepH rangeMax. Temp. (°C)

1–12

pH 1–7 ： 90
pH 7–12 ： 50

Max.
Pressure (MPa)50
pH 1–9 ： 90
pH 9–12 ： 50
pH 1–7 ： 90
pH 7–10 ： 50

20, 30 mmI.D.

：3050 mmI.D.

：20

2–850

2–7.5
＊Refer to the Care & Use Instructions of corresponding product/brand if yours is not listed above.

Inquire us the pressure rating of such product.

3. Shipping solvent
Indicated in the COLUMN INSPECTION REPORT. Replace with this solvent for storage.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM240216AE) 1/2

YMC USER'S MANUAL

4. Mobile phase
・ The correct direction of the solvent flow is indicated by an arrow on the column identification label.
・ Aqueous or non-aqueous solvent can be used as a mobile phase. Repetitive replacement among solvents with large differencein polarities might degrade the column performance. In general, acetonitrile, methanol and tetrahydrofuran (THF) arerecommended for regular use. When using THF as a mobile phase, be mindful of the solvent resistance of your system or tubing(PEEK parts are especially unsuitable for use with THF).
・ Recommendations of pH for column use are shown in the specifications table in section 2. When using the column at pH near
the upper or lower limit, a mobile phase containing 10% concentration of organic solvent should be used. The column lifetime will
shorten under certain conditions by temperature and mobile phase composition.
・ When using Triart C18 ExRS and Pro C18 RS column, its extreamly high hydrophobicity might cause difficulties of replacement
or equilibration with mobile phase containing low concentration organic solvent. Please organic solvent ratio is a guide to thefollowing. When replacing methanol aqueous solution with acetonitrile aqueous soluiton, mobile phase containing less than 20%of acetonitrile may result in irregularities in retention time or peak shapes. In these cases, first replace with 60% acetonitrile
aqueous solution, and then replace with the mobile phase.
［Concerning organic solvent ratio］
Triart C18 ExRS

methanol : more than 15% , lower polar organic solvent : more than 10%

Pro C18 RS

methanol : more than 10% , lower polar organic solvent : more than 5%

5. Column cleaning ( general method )・ Flush the column with solution containing a higher ratio of organic solvent for washing out the compounds that have a great
capacity for retention in the column after using mobile phases not containing buffer salts/additives. Usable concentration oforganic solvent is up to 100%. A cleaning solution containing THF might be effective when removing highly hydrihobic(lipid-soluble) substances that are adsorbed onto the gel.
・ When using mobile phase containing buffer salts/additives, first replace with a water/organic solution containing no buffer
salts/additives (A ratio of water to organic solvent should be set at the same proportions as a mobile phase). Then flush thecolumn in accordance with the method described above. Mobile phase containing about 50 mM or less in buffer salts/additives
can be replaced directly with 60% acetonitrile aqueous solution.
・ Flushing with 100% water after using the column around the pH limit might shorten the column lifetime. Flush the column withwater/organic solution as described above, such as 60% acetonitrile aqueous solution.
・ Once macromolecules such as proteins or polysaccharides are adsorbed onto the gel, they are hardly removed, even if solventswith high eluting capability are used. To avoid contamination of the column by them, conduct sample pretreatment carefully
before introduction into the column.

6. Other environments
・ The upper limit of column pressure is about 30 MPa (4350 psi).
・ Avoid using a column repeatedly near the pressure limit or abrupt change in pressure to prevent shortening of the column life.
・ Adjust the flow rate appropriately because the pressure changes depending on the column length, temperature, types of organicsolvent etc.
・ The upper limit of column temperature is shown in the specifications table in section 2. However, we recommend using thecolumn between 20 – 40 °C, because some conditions of usage such as pH of the mobile phase, might shorten the column
lifetime in particular at the upper temperature limit of the column. When using the column long term under alkaline conditions, werecommend using a low concentration (about 1 – 10 mM) of organic buffer solution with mobile phase (methanol isrecommended) at lower temperature (less than about 30 °C).
・ When using the semi-preparative columns above ambient, irregularities in peak shapes such as peak broadenings and peak
splits might happen, because temperature in the column is not kept uniformly. To avoid those phenomenons, we recommend
preheating the mobile phase.

YMC Karasuma-Gojo Bldg., 284 Daigo-cho, Karasuma Nishiiru Gojo-dori, Shimogyo-ku,
Kyoto 600-8106, Japan
TEL +81-75-342-4515 FAX +81-75-342-4550
URL:www.ymc.co.jp E-Mail:sales@ymc.co.jp(UM240216AE) 2/2