Data Sheet

Agilent 6546
Parameter

MeasureSpecification

MS Sensitivity, positive

Signal-to-noise (S/N) for 1 pg injection of reserpine, while maintaining maximum mass resolution>500:1 RMS in 10 replicates

MS/MS Sensitivity, positive

S/N for 1 pg LC/MS injection of reserpine, monitoring the most intense product ions (174, 195, 397,and 448 m/z) while maintaining maximum mass resolution>1,500:1 RMS in 10 replicates

MS Sensitivity, negative

S/N for 1 pg injection of chloramphenicol, while maintaining maximum mass resolution>500:1 RMS in 10 replicates

MS/MS Sensitivity, negative

S/N for 1 pg LC/MS injection of chloramphenicol, monitoring the most intense product ions(m/z 152, 176, 194, and 257) while maintaining maximum mass resolution>1,500:1 RMS in 10 replicates

MS Accuracy, positive

Measured at the (M+H)+ ion of reserpine (m/z 609.2807) using an internal mass reference<0.8 ppm RMS in 10 replicates

MS/MS Accuracy, positive

Product ion m/z 397.2122 for reserpine<2.0 ppm RMS in 10 replicates

MS Accuracy, negative-Measured at the (M-H) ion of chloramphenicol (m/z 321.0051) using an internal mass reference<0.8 ppm RMS in 10 replicates

MS/MS Accuracy, negative

Product ion m/z 152.0353 for chloramphenicol<2.0 ppm RMS in 10 replicates

Mass resolving power, positive

Full width half mass (FWHM) of spectral peak, with all instrument parameters set by autotune>30,000 FWHM at m/z 118>60,000 FWHM at m/z 2,722

Mass resolving power, negative

FWHM of spectral peak, with all instrument parameters set by autotune>30,000 FWHM at m/z 113>60,000 FWHM at m/z 2,834

Isotope fidelity

Relative isotopic abundance error (RIA error (%) = 100 × (RIAexp – RIAtheo)/ RIAtheo) of the RMS for
10 ESI calibrant ions in positive polarity<5 %Mass range

m/z 50–10,000

Temperature stability

Temperature: 15 to 35 °C (59 to 95 °F) at constant temperature

Maintain 1 ppm mass accuracy(variations <3 °C from calibrationtemperature)

Dynamic range

In spectrum dynamic range on coeluting components while maintaining maximum mass resolution

Up to five decades

Spectral acquisition rate, MSAcquisition speed while maintain maximum resolving power (mode dependent)

50 spectra/second

Spectral acquisition rate, MS/MS

Acquisition speed while maintain maximum resolving power (mode dependent)

30 spectra/second

Polarity switching

Complete cycle of positive and negative spectral acquisition

1.5 seconds

Agilent SWARM Autotune

Flexible automated optimization for different m/z ranges as well as fragile compounds

Data Analysis for the following workflows

Target/Suspect Screening in MassHunter Quantitative Analysis with LC Q-TOF Screener, Qualitative flux analysis in VistaFlux, Foodauthenticity analysis in Mass Profiler Professional (MPP) and MassHunter Classifier, Metabolomics and Lipidomics analysis with MPPand MassHunter Lipid Annotator

Integrated calibrant solution

Automated delivery of calibrant for tuning and mass calibration

Vent-free capillary removal

Isolation valve to allow source and sampling capillary maintenance while maintaining system vacuum.

All chemical sensitivity, chemical
accuracy, and resolution specifications
are achieved in manufacturing, andinstrument performance data aresupplied with shipment. All specification
values are achieved after autotune, anddo not require manual optimization.
These specifications are not standardinstallation specifications for the Agilent
Q-TOF. The Agilent high-resolution
accurate mass Q-TOF instruments
are tested and installed in accordancewith standard performance tests, as
described in the Agilent installation
manual.

www.agilent.com/chem
This information is subject to change without notice.
© Agilent Technologies, Inc. 2019
Printed in the USA, January 15, 20195994-0609EN