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Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

Annexin-Vivo™ 750
Product Number: NEV11053

DESCRIPTION: Annexin-Vivo™ 750 is a targeted fluorescence imaging agent comprising a selective protein, Annexin
A5 and a near-infrared (NIR) fluorochrome. This agent has been developed to enable in vivo visualization and quantification of
the membrane-bound phospholipid, phosphotidylserine, exposed in the outer leaflet of the cell membrane lipid bilayer during
the early stages of apoptosis.
MATERIAL (Ready to use: No dilution required)

CONTENTS: Each vial contains 10 mouse doses ofAnnexin-Vivo 750 in solution. Annexin-Vivo 750 is supplied in1000 µL of 25 mM Hepes/NaOH, 140 mM NaCl, pH 7.4.
The packaged material provides sufficient reagent for imaging
approximately 10 mice (weighing ~25 grams each) when using
the recommended dose of 100 µL of Annexin-Vivo 750 permouse.

Table 1. Annexin-Vivo 750 Characteristics
Property
SpecificationMW
35,000 g mol-1
Fluorescence1
 Excitation
755 nm Emission
772 nmAbsorbance
748 nmPurity2>95 %AppearanceBlue solution

1. Absorbance and fluorescence maxima of Annexin-Vivo 750 in water
2. As determined by RP-HPLC and measuring absorbance at 750 nm.

PROPERTIES: The physical characteristics of AnnexinVivo 750 can be found in Table 1 and Figure 1.

STORAGE & HANDLING:
Upon receipt, Annexin-Vivo 750
should be IMMEDIATELY STORED AT
2-8 oC AND PROTECTED FROM LIGHT.

When stored and handled properly, Annexin-Vivo 750
is stable for up to six months.

Before opening the vial check to ensure that thesolution is present at the bottom of the vial.

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Figure 1. Absorbance and fluorescence emission spectra ofAnnexin-Vivo 750 in 1x PBS.

IN VIVO IMAGING AND APPLICATIONS:The generally recommended procedure for in vivo imaging with Annexin-Vivo 750 is administration via intravenous
injection and imaging 2 hours post injection.
Annexin-Vivo 750 will clear from tissues after ~3 days after which repeat injection and imaging may be
performed for longitudinal studies. However it is recommended that a pre-injection baseline image be taken
prior to reinjection and imaging.
Annexin-Vivo 750 enables imaging of therapeutic response in oncology models, and has applications in cardiovasculardisease, transplant rejection, and pancreatitis models.

NOTES:
PerkinElmer’s Annexin-Vivo 750 is intended for research purposes only and is not for human use. It must be used byor directly under the supervision of a technically qualified individual experienced in handling potentially hazardous
materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement fromPerkinElmer.

PerkinElmer, Inc.
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P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
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