07

Core-Shell Columns

Meteoric Core ----------------------------------------- 72-74
Ordering Information------------------------------------- 74

07

Core-Shell Columns

Meteoric Core
Meteoric Core is Core-Shell column with outstanding resolution for UHPLC & HPLC. Meteoric Core can be used across a wide pH
range and provides excellent peak shape for basic and coordination compounds compared to conventional columns or competitors’.
This feature enables smoother method development. Meteoric Core is ideal for ultrafast and high resolution analysis. Meteoric Corecan reduce its backpressure by half compared to sub-2 μm columns with the same resolution as this. Meteoric Core can be used withconventional HPLC as well as UHPLC.

Core-Shell columns with outstanding resolution for UHPLC & HPLCFeatures
Ultrafast analysis and excellent r esolution
Excellent peak shape on basic and c oordination compounds
Wide usable pH range
Low column bleeding and ideal for LC/MS

Porousshell layer

0.5 μmCore-Shell Columns

Core

1.7 μm

2.7 μm

0.5 μmMeteoric CoreParticle image structure

SpecificationsMeteoric Core C18Base

2.7

Pore size (Å)
2Specific surface area (m /g)

80

16080

150

90

150Bonding

Trifunctional
7Endcapping

72

Meteoric Core C8Core-Shell type silica gel

Particle size (μm)

Carbon content (%)

Meteoric Core C18 BIO

55Yes

Usable pH range

1.5-101.5-101.5-9

USP classification

L1L1L7

Advantages of Core-Shell column packing material
Van Deemter Curves : Correlation between linear velocity and column efficiency

Column Pressure : Correlation between linear velocity and column backpressure

0.025

Fully porous C18 5 μm

120Core-Shell type sub-2 μm
Brand G6100

Column pressure (MPa)

HETP (mm/plates)

0.020

Core-Shell type sub-2 μm
Brand I14
Pressure limit of sub-2 μm

0.015

Fully porous C18 3 μm
0.010

Meteoric Core C18 2.7 μm
Fully porous C18 sub-2 μm

0.005

Core-Shell type sub-2 μm
Brand I14

Core-Shell type sub-2 μm
Brand G6

Fully porous C18 sub-2 μm
80Pressure limit of Meteoric Core60

Pressure limit of conventional HPLC systems

4020

Meteoric Core C18 2.7 μm

Fully porous C18 3 μm

Fully porous C18 5 μm00.000
0100.1

20.2

30.3

4567
Interstitial liner velocity (mm/sec)

89100.4

0.9

1.0

1.1

0.5

0.6

0.7

0.8

111.2

0200.2

40.4

Flow rate (mL/min) for Meteoric Core C18 50 X 2.1 mmI.D.

60.6

8101214
Interstitial liner velocity (mm/sec)

16

180.8

1.8

2.0

1.0

1.2

1.4

1.6

Flow rate (mL/min) for Meteoric Core C18 50 X 2.1 mmI.D.

Meteoric Core C18 has high column efficiency which is almost equivalent to sub-2 μm columns over a wide
range of flow rate.
The operating pressure of Meteoric Core is one half to one fifths of sub-2 μm Core-Shell type columns.
High throughput analysis using Meteoric Core could be expected even with longer length columns since theusable maximum flow rate of it is higher than competitors’ sub-2 μm Core-Shell.

Column: 50 X 2.0 or 2.1 mmI.D.
Eluent: acetonitrile/water (60/40)
Temperature : 25°C
Sample: butyl benzoate

Ultrafast separation of Parabens: Typically difficult-to-separate geometric isomers

HPLCHPLC∙UHPLC

0.4 mL/min

mAU
1000123 45 613.0 MPa(1890 psi)

0H140116F

123456789 min

1. Methyl p-hydroxybenzoate
2. Ethyl p-hydroxybenzoate
3. Isopropyl p-hydroxybenzoate
4. Propyl p-hydroxybenzoate
5. Isobutyl p-hydroxybenzoate
6. Butyl p-hydroxybenzoate

Core-Shell Columns

Ultrafast analysis and excellent resolution

Conventional
fully porous C183 μm

202.2mAU

Meteoric Core C18
2.7 μm231000.4 mL/min
16.3 MPa(2360 psi)

00mAU

0.8 mL/min

H140116E12356 min

131000433.6 MPa(4870 psi)

0H140124A

123 minMeteoric Core C18 can shorten the analysis time by two thirds compared to the conventional fully porous
C18 column with the same column dimension and under the same analysis condition. Moreover, it maintains
the theoretical plate number at a two times faster flow rate. It allows us to decrease analysis time by one
thirds while maintaining resolution, and at an operating pressure less than 5000 psi.

Column: 150 X 3.0 mmI.D.
Eluent: acetonitrile/water (50/50)
Temperature : 30°CDetection: UV at 270 nm

73

07Core-Shell Columns
Peptide/Protein separation
Meteoric Core C18 BIO with wider pore size: Appropriate for separation of peptides/proteins whose molecularweight are up to 30,000
Peptides (MW 574 - 3,465)

Peptides/Proteins (MW 4,300 - 25,700)

mAU

mAU

80

80

60

240

12 3

613 457460

540

20

20

0H140303A

05

1015min

1. BAM-12P(MW 1,425)
2. [D-Ala2,Met5]-Enkephalinamide (MW 587)
3. Met-Enkephalin(MW 574)(MW 588)
4. [D-Ala2,Met5]-Enkephalin
5. α-Endorphin(MW 1,746)
6. γ-Endorphin(MW 1,859)
7. β-Endorphin(MW 3,465)

0H140219D

051015min

1. Cytochrome c(MW 12,400)
2. Insulin (bovine)(MW 5,700)
3. Amyloid β-protein(MW 4,300)
4. Lysozyme(MW 14,000)
5. α-Chymotrypsinogen A (MW 25,700)

Core-Shell Columns

Column: Meteoric Core C18 BIO 2.7 μm
150 X 2.1 mmI.D.
Eluent: A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
15-55%B (0-15 min), 55%B (15-17 min)Flow rate: 0.2 mL/min
Temperature : 40°C
Detection: UV at 220 nm
Pressure: 14.9-16.1 MPa (2160-2330 psi)

Column: Meteoric Core C18 BIO 2.7 μm
150 X 2.1 mmI.D.
Eluent: A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
25-70%B (0-15 min), 70%B (15-17 min)Flow rate: 0.2 mL/min
Temperature : 40°C
Detection: UV at 220 nm
Pressure: 12.8-16.1 MPa (1860-2330 psi)

Ordering Information –Columns–
Meteoric Core C18Phasedimension

Column I.D.
(mm)

80 Å2.7 μm

2.1
3.0
4.6Column length (mm)30
CAS08SQ7-03Q1PT
CAS08SQ7-0303PT
CAS08SQ7-0346PT

50
CAS08SQ7-05Q1PT
CAS08SQ7-0503PT
CAS08SQ7-0546PT

75
CAS08SQ7-L5Q1PT
CAS08SQ7-L503PT
CAS08SQ7-L546PT

100
CAS08SQ7-10Q1PT
CAS08SQ7-1003PT
CAS08SQ7-1046PT

150
CAS08SQ7-15Q1PT
CAS08SQ7-1503PT
CAS08SQ7-1546PT

100
CAW16SQ7-10Q1PT
CAW16SQ7-1003PT
CAW16SQ7-1046PT

150
CAW16SQ7-15Q1PT
CAW16SQ7-1503PT
CAW16SQ7-1546PT

100
COS08SQ7-10Q1PT
COS08SQ7-1003PT
COS08SQ7-1046PT

150
COS08SQ7-15Q1PT
COS08SQ7-1503PT
COS08SQ7-1546PT

Meteoric Core C18 BIO
Phase
dimension

Column I.D.
(mm)

160 Å
2.7 μm

2.1
3.0
4.6

Column length (mm)30
CAW16SQ7-03Q1PT
CAW16SQ7-0303PT
CAW16SQ7-0346PT

50
CAW16SQ7-05Q1PT
CAW16SQ7-0503PT
CAW16SQ7-0546PT

30
COS08SQ7-03Q1PT
COS08SQ7-0303PT
COS08SQ7-0346PT

50
COS08SQ7-05Q1PT
COS08SQ7-0503PT
COS08SQ7-0546PT

75
CAW16SQ7-L5Q1PT
CAW16SQ7-L503PT
CAW16SQ7-L546PT

Meteoric Core C874

Phase
dimension

Column I.D.
(mm)

80 Å
2.7 μm2.1
3.04.6

Column length (mm)75
COS08SQ7-L5Q1PT
COS08SQ7-L503PT
COS08SQ7-L546PT