Instructions to Authors | 1

Index
Aims and scope
Editorial policy| General requirements | Publication ethics / response to possible misconduct | Retraction |Permissions | Authorship | Conﬂict of interest | Copyright | Use of human subjects or animals in
research | Disclaimer | Page charges | Color charges | Correction of English | Declaration ofGenerative AI in scientiﬁc writing |Submission to public databases| Nucleotide and amino acid sequences | Raw sequence reads by high-throughput sequencing |Structural determinations | Microarray data | Referencing data in your article through tagging
identiﬁers or accession numbers |Manuscript submission
Review and publication process| Review process | Notiﬁcation of acceptance | Proofs |Share Links | Reprints |Organization and format| Manuscript type | Errata | Style | Title and short title | Authors | Correspondent footnote | Author
contributions| Key words | Abstract | Graphical Abstracts | Introduction | Materials andMethods | Results | Discussion | Acknowledgments | References | Tables | Figures | Figure legends |Supplementary materials | Data in Brief | Highlights | AudioSlides | Nomenclature of Microorganisms|

Aims and scope
The Journal of Bioscience and Bioengineering (JBB) aims to contribute to the advancement and dissemination ofknowledge and technology in the ﬁelds of bioscience and biotechnology. JBB publishes papers on a broad rangeof topics in the areas of enzymology, physiology and biotechnology of microbes, plants, and animals; genetics,
molecular biology, and gene engineering; brewing and food technology; environmental biotechnology;
biochemical engineering; cell and tissue engineering; protein engineering; biomedical engineering; andbioinformatics. Genomics, systems biology, and structural biology, which hold much promise for the future, arealso within the scope of JBB.
The journal only considers submissions that report on the most signiﬁcant and fundamental advances in theﬁeld of bioscience and bioengineering. Manuscripts that describe optimizations related to phenomena of
narrow interest using conventional statistical approaches and/or describe exclusively computer simulation withconventional enzyme reaction models or molecular dynamics will be rejected without peer review.

Instructions to Authors | 2

The brewing and food technology section welcomes researches that provide fundamental advances on
fermentation, fermented foods and their process developments. Basic studies solely treating the chemical
and/or medicinal aspects of food derivatives prepared by conventional pretreatment techniques are notappropriate for JBB.
The cell and tissue engineering section covers research on cell culture engineering as it relates toregenerative medicine. Topics include the technologies for stem cell culture such as mass production,
diﬀerentiation control, and tissue reconstruction. Studies in the ﬁeld of pure cell biology and embryology are
not appropriate for JBB.
The biomedical engineering section covers topics related to bioprocess engineering in the ﬁeld of medicine,including biomaterials, scaﬀolds, artiﬁcial organs, drug delivery systems, microﬂuidics, and micro-fabrication,
but not deals with the topics mainly focusing on mechanical engineering. Studies in the ﬁeld of basic medicine,
pharmaceutical sciences, dentistry, and surgical technique are not suitable for JBB. The journal does notconsider pathological, clinical, and epidemiological researches.
Posting of preprints on preprint servers will not threaten the consideration of your manuscript for publication.
However, the authors should carefully check and follow the Elsevier’s guideline on preprint and show the DOI
number in your cover letter if applicable.
The journal accepts only those papers that are most signiﬁcant to the ﬁeld of bioscience and bioengineering.
Thus, manuscripts focusing only on optimization of some objective function based on a conventional statistical
approach will be rejected without peer review.
►Page Top

Editorial policy
General requirements
Manuscripts submitted to the journal must represent reports of unpublished original research, which have notbeen published in any language and are not under consideration for publication elsewhere in any medium.
Related works (i.e., articles describing related work by the authors or arising from the same research project,or with any overlap of content to the submitted manuscript) that have been published, or are in press, or
submitted, elsewhere must accompany the submission. The submission for publication must be approved by all
authors.
Publication ethics / response to possible misconduct
JBB expects authors to adhere to recognized ethical codes such as those set out in “Responsible research
publication: international standards for authors”. Similarly, it expects its editors to follow the international
standards for editors (see Responsible research publication: international standards for editors) and theCommittee on Publication Ethics (COPE) Core Practices. It expects peer reviewers to follow the COPE Ethical
Guidelines for Peer Reviewers.
JBB is a member of COPE and will follow its recommended procedures for handling cases of possible (i.e.,
alleged or suspected) misconduct. These are described in the COPE ﬂowcharts. Submissions to JBB may bechecked by CrossCheck, a text-matching system which can detect plagiarism and redundant publication. In

Instructions to Authors | 3

cases of possible serious misconduct (e.g., data fabrication, major plagiarism) the journal may contact theauthors’ institution and request an investigation.
Retraction
The editor(s) and publishers of a scholarly journal such as JBB are responsible for ensuring that it does notinclude misleading or fraudulent information. Therefore, JBB follows the COPE guidelines on retractions andwill retract articles that are unreliable, plagiarized or constitute redundant publication.
Permissions
The corresponding author must obtain permission from the copyright owner to reproduce ﬁgures or tables thathave been published elsewhere and credit the sources in the relevant ﬁgure legend or table footnote of themanuscript.
Authorship
Authorship credit should be based on substantial contributions to the overall design and execution of the work.
Our authorship policies conform to the guidelines of the International Committee of Medical Journal Editors(ICMJE). The ICMJE recommends that authorship be based on the following 4 criteria:
Substantial contributions to the conception or design of the work; or the acquisition, analysis, orinterpretation of data for the work; AND
Drafting the work or revising it critically for important intellectual content; AND
Final approval of the version to be published; AND
Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy
or integrity of any part of the work are appropriately investigated and resolved.
The corresponding author (the single person to whom all the correspondence on the manuscript should be
addressed) is responsible for ensuring that all contributors meet the authorship criteria and for informing all
authors of the manuscript’s status throughout the review and publication process.
The corresponding author is required to link his/her ORCID to the Editorial Manager proﬁle. For details
about ORCID, please visit https://orcid.org/.
The order of authorship on the byline should be a joint decision of the co-authors. Statements regarding equal
contributions by two or more authors (e.g., the ﬁrst two authors contributed equally to this work) arepermitted as footnotes to bylines. A change in authorship after ﬁrst submission requires an agreement signedby all authors. JBB will follow the COPE ﬂow charts on adding or removing authors.
Membership of the Society for Biotechnology, Japan (SBJ) is not a prerequisite for submission.
Deceased authors: it is permissible to include a deceased author if that person made a signiﬁcant contribution
to the work and it is the wish of the other authors to acknowledge this. The aﬃliation where the work wasdone should be listed. A footnote reporting the date of death should be added. Alternatively, the individual’scontribution may be mentioned in the Acknowledgment section. If possible, permission to include the deceased
author’s name or to mention them in the Acknowledgments should be obtained from their family.
Conﬂict of interest
Authors are requested at the time of submission to disclose any commercial association as well as consulting,stock ownership, equity interests, or patent-licensing arrangements that might pose a conﬂict of interest inInstructions to Authors | 4connection with their submitted work. Sources of any ﬁnancial support for the project must be credited in the
Acknowledgments section.
Copyright
JBB is copyrighted by the Society for Biotechnology, Japan (SBJ), and authors are considered to havetransferred their rights to SBJ upon acceptance. Requests for any reproduction or translation of material
published in JBB should be submitted to the SBJ Business Oﬃce (jbb@sbj.or.jp). This request must include astatement of intended use as well as explicit speciﬁcation of the material to be reproduced.
Use of human subjects or animals in research
Manuscripts reporting experiments on human subjects should clearly state in the article that the researchcomplied with the Helsinki Declaration of 1964, as revised in 2013 and with the ethical standards of the
responsible committee on human experimentation (institutional and national) and should state the name of thecommittee that approved the work. Experiments using animals should be conducted in accordance with therelevant institutional and national standards; authors are required to indicate whether the guidelines for thecare and use of laboratory animals were followed.
Disclaimer
SBJ assumes no responsibility for the statements and opinions advanced by the contributors to the Society’s
publications. Editorial views do not necessarily represent the position of SBJ. The use of trade names does not
imply endorsement by SBJ.
Page charges
Submissions from Japan are subject to page charges of 6,050 JPY incl. tax per printed page. An invoice will besent from the SBJ Oﬃce to the author once the article has been published in print. SBJ does not require page
charges for articles that meet the following conditions: 1) the research was conducted outside Japan AND 2)
the work was not funded by a Japanese governmental/non-governmental organization, private company or
academic institution.
Color charges
Color ﬁgures are published on the web at no additional charge, regardless of whether or not they are
reproduced in color in the printed version. Authors may choose to publish their ﬁgures in color or in black-andwhite in the printed version. The corresponding author will receive a request form for the color option from
SBJ after the article is accepted for publication. For color reproduction in print, authors will be charged 44,000
JPY incl. tax per printed page.
Correction of English
SBJ strongly recommends authors who are unsure of proper English usage to have their manuscript checked by alanguage editing service prior to submission. Manuscripts that do not conform to standard English usage may berejected without peer review and returned to the authors. If a reviewer or editor suggests that a manuscript
under review requires correction it may be sent for language correction at the editor’s discretion. In such case,the costs must be borne by the author. No waivers will be considered.
For authors who require information about language editing and copyediting services pre- and post-submission,
English Language Editing service is available from Elsevier’s WebShop or visit Elsevier’s customer support
site for more information. Please note SBJ neither endorse nor take responsibility for any products, goods orInstructions to Authors | 5

services oﬀered by outside vendors through their services or in any advertising.
Declaration of Generative AI in scientiﬁc writing
Where authors use generative artiﬁcial intelligence (AI) and AI-assisted technologies in the writing process,authors should:
Only use these technologies to improve readability and language, not to replace key researcher tasks suchas interpreting data or drawing scientiﬁc conclusions.
Apply the technology with human oversight and control, and carefully review and edit the result, as AI cangenerate authoritative-sounding output that can be incorrect, incomplete or biased.
Not list AI and AI-assisted technologies as an author or co-author, or cite AI as an author. Authorship
implies responsibilities and tasks that can only be attributed to and performed by humans, as outlined in
Elsevier’s AI policy for authors.
Disclose in their manuscript the use of AI and AI-assisted technologies in the writing process by following
the instructions below. A statement will appear in the published work. Please note that authors areultimately responsible and accountable for the contents of the work.
＜Disclosure instructions＞
Authors must disclose the use of generative AI and AI-assisted technologies in the writing process byadding a statement at the end of their manuscript in the core manuscript ﬁle, before the References list.
The statement should be placed in a new section entitled ‘Declaration of AI and AI-assisted technologies
in the writing process’.

Statement: During the preparation of this work the author(s) used [NAME TOOL / SERVICE] in order to[REASON]. After using this tool/service, the author(s) reviewed and edited the content as needed andtake(s) full responsibility for the content of the publication.
This declaration does not apply to the use of basic tools for checking grammar, spelling, references etc.
If there is nothing to disclose, there is no need to add a statement.
►Page Top

Submission to public databases
Nucleotide and amino acid sequences
Before publication, authors must deposit novel nucleotide and/or amino acid sequence data to one of themembers of the International Nucleotide Sequence Database Collaboration (INSDC): DNA Data Bank of Japan(DDBJ); European Nucleotide Archive (ENA); and GenBank, National Center for Biotechnology Information(NCBI). The accession number(s) should be listed in a separate paragraph at the end of the Materials andMethods section for Regular papers.
Raw sequence reads by high-throughput sequencing
Before publication, authors must deposit raw sequence reads in the Sequence Read Archive for the case of
amplicon, meta-‘omic, and genomic nucleic acid data. Authors are encouraged to comply with community
metadata standards, such as the “Minimal Information about any (X) Sequence” (MIxS) checklist, whensubmitting the raw data to one of the members of the International Nucleotide Sequence Database

Instructions to Authors | 6

Collaboration (INSDC). The accession number(s) should be listed as mentioned in the above section “Nucleotide
and amino acid sequences”.
Structural determinations
Papers describing new structures of macromolecules must include entry codes assigned by the Protein Data
Bank (https://www.rcsb.org/). The atomic parameters and structure-factor amplitudes should be released
when the manuscript is published.
Microarray data
Results based on microarray experiments should satisfy the Minimal Information About a Microarray
Experiment (MIAME) standard criteria and must be deposited in the appropriate public databases:
ArrayExpress, Gene Expression Omnibus (GEO) or Center for Information Biology Gene Expression Database(CIBEX). The accession number(s) should be listed in a separate paragraph at the end of the Materials andMethods section for Regular papers.
Referencing data in your article through tagging identiﬁers or accession numbers
Authors are encouraged to connect articles with external databases, giving their readers one-click access torelevant databases that help to build a better understanding of the described research. Please refer torelevant database identiﬁers using the following format in your article: Database: xxxx (e.g., TAIR: AT1G01020;
CCDC: 734053; PDB: 1XFN). See https://www.elsevier.com/databaselinking for more information and a fulllist of supported databases.
►Page Top

Manuscript submission
Authors are requested to submit their manuscripts electronically, using Editorial Manager ® . Detailed
instructions are available in Tutorials for Authors, Reviewers, and Editors (WORD/PDF). Video Library is alsoavailable, which allows Editors, Authors and Reviewers to familiarize themselves with Editorial Manager
through short learning blocks at their own pace, in their own time.
The online submission process must be carried out by the corresponding author. The corresponding author is
responsible for providing accurate data in the submission process. Registration using personal data of anotherperson including email address infringes publication ethics.
After registration, authors will be asked to upload their article, an extra copy of the abstract, associatedartwork, and information (email, position, and aﬃliation) of all coauthors. The submission tool will generate a
PDF ﬁle to be used for the reviewing process. The corresponding author and coauthors will receive an automatic
reply and a manuscript number for future correspondence. A cover letter and Author Agreement must be
submitted online together with the manuscript. The cover letter must address the following points:
1) all authors have read the submitted version of the manuscript and agree to submit the work to JBB,
2) the work has not been published before and is not being considered for publication by another journal,3) the novelty and signiﬁcance of the submitted work must be brieﬂy described,4) the transfer of copyright from the author to the Society for Biotechnology, Japan (SBJ) (if the article isInstructions to Authors | 7

accepted for publication),5) whether the manuscript has been checked by language editing services (or a native English speaker) prior to
submission (please attach certiﬁcate, if applicable).
If the manuscript is a resubmission, the former manuscript number should be indicated. Please note any use of apreprint server in the cover letter and include a link to the preprint.
►Page Top

Review and publication process
Review process
All manuscripts are considered to be conﬁdential and are reviewed by members of the Editorial Board, andqualiﬁed referees. Reviews are normally completed within 3 to 4 weeks from receipt of the manuscript. The
authors will be notiﬁed of the editor’s decision to accept, reject, or reconsider after revision. Note that
manuscripts may be editorially rejected without external peer review, on the basis of lack of conformity to thestandards set out in these instructions. If revisions are requested, authors must submit the revised version
along with a point-by-point response to each of the reviewers’ and editor’s suggestions. If the revision is not
submitted within 60 days any revised version will be considered as a new submission. Additional time forrevision may be granted upon request, at the discretion of the editor. Manuscripts that have been rejected orwithdrawn after being returned for modiﬁcation may be resubmitted with appropriate modiﬁcations based on
the editor’s and reviewers’ comments. In such cases, the revised version must be submitted as a new submission,accompanied by the former manuscript number and detailed response to the original reviewers, using thejournal’s online submission system, and should not be sent to the original editor directly.
Notiﬁcation of acceptanceOnce a decision has been made on an article, the author(s) will be notiﬁed. If the article is accepted, it will beentered into Elsevier’s production tracking system and the corresponding author will receive an
acknowledgement letter containing the production reference number, accompanied by an oﬀprint order form
and a copyright transfer form.
The production reference number along with the corresponding author’s last name can be used to track thestatus of the article at Track Paper.
Please note that the production reference number is diﬀerent from the editorial reference number assigned by
the Editor.
Proofs
The corresponding author will receive one set of page proofs in PDF format from Elsevier’s production
department. The email address for the return of corrected proofs will be indicated in the proof e-mail.
Share LinksJBB oﬀers Share Links. This is a customized link which authors receive for their newly-published article on
ScienceDirect. The link provides 50 days’ free access to their article – after that, the usual access rules apply.
Anyone clicking on the Share Link within the 50-day period will be taken directly to the article with no sign up
or registration required. Share Links are ideal for sharing articles via email and social media.

Instructions to Authors | 8

You can ﬁnd out more about Share Links on Elsevier.com.
Reprints
Prior to publication, authors will be provided with a Share Link – a personalized URL providing 50 days’ freeaccess to download the copy of their newly-published article on ScienceDirect. After publication, authors canorder paper oﬀprints via the Elsevier Author WebShop.
►Page Top

Organization and format
Manuscript type
Regular papers: Full-length papers describing the results of original research. Manuscripts should be written
to emphasize clearly and concisely the novel aspects of the information reported, and preferably should notexceed 25 double-spaced pages of text (ca. 25 lines per page, including references). Authors are encouraged torestrict ﬁgures and tables to essential data, preferably no more than 6 ﬁgures and/or tables combined.
Reviews: Reviews survey recent developments in a topical area of research and should be sharply focused andbalanced accounts of progress in ﬁelds of interest to readers of the journal. Reviews should not generally
exceed 35 double-spaced pages of text (limit of 10,000 words, including references and ﬁgure legends) with nomore than 70 references. Authorship is normally by invitation, although we are keen to receive proposals forprospective articles from authors. Authors considering submission of a review article must ﬁrst send a brief
synopsis (approximately 500 words), citing key references, to the Journal Oﬃce at (jbb@sbj.or.jp) (please do
not submit the completed manuscript prior to this consultation). If the proposal is accepted, authors may
submit the manuscript via Editorial Manager®.
Letters to the editor: Letters to the editor are conﬁned to discussions of articles that have appeared in JBB
within the previous three months. Although the format is ﬂexible, they are peer-reviewed, and are usually
substantially edited by JBB editors in consultation with the authors. Letters should not exceed 2 printed pages
including ﬁgures and tables.
ErrataErratum provides a means of correcting errors that occurred during the publication process, such as
misspelling, mislabeling in a ﬁgure, a missed word, and so on. Please contact the SBJ Business Oﬃce
(jbb@sbj.or.jp) before you send Errata.
►Page TopStyle
The whole manuscript (including references, ﬁgure legends, and table footnotes) must be typed in 12 point anddouble-spaced (a minimum of 6 mm between lines, ca. 25 lines per page). Times or Times New Roman font isrecommended. Format your documents for A4 size (210 × 297 mm). Number each page at the bottom, beginningwith the title page. Manuscript pages should have line numbers. Items should be provided in the following
order: Title page, Abstract, Introduction, Materials and Methods, Results, Discussion, Acknowledgments,
References, Figure legends, Figures, and Tables. Each ﬁgure and table ﬁle should be uploaded individually. For

Instructions to Authors | 9

technical reasons, symbols, shading, double underlines, and wavy lines may not be used in text body or tables.
Manuscripts that do not conform to these instructions may be rejected without review and returned to theauthors for reformatting. The Manuscript File size may not exceed 150 MB. The total size of your submission
ﬁles may not exceed 700 MB.
Title and short title
The title should be concise and informative. Complete sentences should be avoided. Numbered series titles are
not allowed, because the journal guarantees neither the publication of each title in the series nor the order ofpublication. The title should not contain any jargon or nonstandard abbreviations. Avoid starting the title with
A, An or The (as these are ignored for indexing purposes). Provide a short title of no more than 54 characters
including spaces for Regular papers and Reviews. The short title appears at the upper corner of publishedarticle pages.
Authors
The full name and the complete mailing address of each author should be given. Place superscript numbers after
the name of each author to indicate the aﬃliation. An asterisk should follow the name of the corresponding
author. Multiple corresponding authors are not allowed. State the aﬃliation where the research was performed(rather than the author’s current location). If an author has moved, the current address may be given in afootnote.
Correspondent footnote
A single e-mail address, a single telephone number for the corresponding author should be provided.
Author contributions
For transparency, we require corresponding authors to provide co-author contributions to the manuscript using
the relevant CRediT roles. The CRediT taxonomy includes 14 diﬀerent roles describing each contributor’s
speciﬁc contribution to the scholarly output. The roles are: Conceptualization; Data curation; Formal analysis;
Funding acquisition; Investigation; Methodology; Project administration; Resources; Software; Supervision;
Validation; Visualization; Roles/Writing – original draft; and Writing – review & editing. Note that not all roles
may apply to every manuscript, and authors may have contributed through multiple roles. More details and an
example.
Key words
A list of 5 to 10 key words that will be useful for indexing or searching must be included on the title page.
Avoid abbreviations. General terms, such as “activity”, “culture”, “enzyme” and so on, should not be used unlessqualiﬁed, e.g., “surface activity”, “fed-batch culture”, and “enzyme stability”. These may be edited andcorrected by the journal at the publication stage.
►Page Top

Abstract
An abstract should provide the context or background for the study and state the purpose of the research,
scope of the experiments, major ﬁndings, and principal conclusions. References in the abstract must be cited infull using the style “Inoue et al., J. Biosci. Bioeng., 117, 539-543 (2014)”. The abstract should be written in thepast tense as a single paragraph not exceeding 250 words for a regular article. Headings are not acceptable.

Instructions to Authors | 10

Graphical Abstracts
Although a graphical abstract is optional, its use is encouraged as it draws more attention to the online article.
The graphical abstract should summarize the contents of the article in a concise, pictorial form designed tocapture the attention of a wide readership. Graphical abstracts should be submitted as a separate ﬁle in the
online submission system. Image size: Please provide an image with a minimum of 531 × 1328 pixels (h × w) or
proportionally more. The image should be readable at a size of 5 × 13 cm using a regular screen resolution of 96
dpi. Preferred ﬁle types: TIFF, EPS, PDF or MS Oﬃce ﬁles. You can view Example Graphical Abstracts on
Elsevier’s information site.
Authors can make use of Elsevier’s Illustration Services to ensure the best presentation of their images and inaccordance with all technical requirements.
Introduction
Provide the context and suﬃcient background information for the study. Do not include data or conclusions
from the work being reported. Subheadings should not be used and an exhaustive review of the literature
should be avoided.
Materials and Methods
Provide suﬃcient technical information to allow the experiments to be reproduced. Previously publishedmethods should be cited, and truly novel procedures should be described in detail. If a manuscript concerns acommercial product, the manufacturer’s name and their location (city, state, and country) must be indicated inthis section. Do not number headings or subheadings and avoid third-level headings.
Results
The results of experiments and representative data should be stated clearly and precisely in the past tense. Do
not repeat experimental details previously provided in the Materials and Methods section. Subheadings can beused.
Discussion
Discussion should be restricted to interpretation of the results. Subheadings can be used. When appropriate,
you may combine the Results and Discussion section.
Acknowledgments
Acknowledgments should be brief in a single paragraph and should precede the references. Personalacknowledgments precede those of agencies and institutions. List all sources of funding. The complete name of
the funding agencies and the grant numbers should be given. The sentence should begin: This work was
supported by the Japan Society for the Promotion of Science (grant number xxxx).
►Page Top
References
All listed references must be cited in the text. Arrange the citations parenthetically in the order of theirappearance in the text and number consecutively.
The following types of reference may be cited in the References section:
Journal articles (both print and online)

Instructions to Authors | 11

Books (both print and online)
Book chapters (book title is required)
Published conference proceedings
Meeting abstracts (from published abstract books or journal supplements)
Scientiﬁc and technical reports
Patents
Dissertations and theses
Legal documents
Government/Organization reports
Company publications
Letters to the editor
In-press journal articles, books, and book chapters (publication title is required)Abbreviate journal names according to the PubMed Journals Database (National Library of Medicine, National
Institutes of Health). Use periods after abbreviations. One-word titles (e.g., Biochemistry) are neverabbreviated. In-press references should indicate author name(s), article title, journal title, volume and page
numbers and/or DOI, and online publication date. In-press articles should be submitted as the item type
‘Supporting File’.
The following types of references are not valid for listing:Unpublished data
Manuscripts submitted (but not yet accepted)
Unpublished conference presentations (e.g., poster or report)
Personal communications
Patent applications and patents pending
Computer software, databases, and websites
References to such sources should be made parenthetically in the text.
Examples for references cited in the text:… was observed (Murata, K. and Hashimoto, W., unpublished data).
… available in the GenBank database (http://www.ncbi.nlm.nih.gov/genbank/index.html).
List all authors when there are 12 or fewer; if more than 13, list the ﬁrst 10 names followed by “and other X
authors”. Follow the style shown in the examples below.
1. Takahashi, H. and Honda, H.: Prediction of peptide binding to major histocompatibility complex class II
molecules through use of boosted fuzzy classiﬁer with SWEEP operator method, J. Biosci. Bioeng., 101, 137–141(2006).
2. Ashiuchi, M. and Misono, H.: Poly-γ-glutamic acid, pp. 123–174, in: Fahnestock, S. R. and Steinbüchel, A.
(Ed.), Biopolymers, vol. 7. Wiley-VCH, Weinheim (2002).
3. Ivanova, N., Sorokin, A., Anderson, I., Galleron, N., Candelon, B., Kapatral, V., Bhattacharyya, A., Reznik,G., Mikhailova, N., Lapidus, A., and other 13 authors: Genome sequence of Bacillus cereus and comparative
analysis with Bacillus anthracis, Nature, 423, 87–91 (2003).

Instructions to Authors | 12

4. Ohtomo, M., Kimura, K., Watanabe, S., and Toeda, K.: Production of components containing γ-aminobutyricacid from rice bran by Lactobacillus brevis IFO12005, Seibutsu-kogaku, 84, 479–483 (2006) (in Japanese).
5. Yamazaki, H., Gotou, S., Ito, K., Kohashi, S., Goto, Y., Yoshiura, Y., Sakai, Y., Yabu, H., Shimomura, M.,
and Nakazawa, K.: Micropatterned culture of HepG2 spheroids using microwell chip with honeycomb-patterned
polymer ﬁlm, J. Biosci. Bioeng., http://dx.doi.org/10.1016/j.jbiosc.2014.03.006 (available online 16 April 2014).

♦EndNote output style ﬁles are available for Journal of Bioscience and Bioengineering.
⇒Download the Journal of Bioscience and Bioengineering style►Page Top
TablesTables should be numbered consecutively with Arabic numerals in order of appearance in the text. Type eachtable double-spaced on a separate page with a short descriptive title typed directly above and with essential
footnotes below. Tables must be made using the Microsoft Word table function.
Figures
It is the author’s responsibility to provide artwork for all illustrations, both line and halftone (i.e.,
photographs). Figures must be restricted to the minimum and numbered consecutively with Arabic numerals.
Multipanel ﬁgures should be labeled with upper-case, bold letters (A, B, C, etc.). Photomicrographs should haveinternal scale markers. Lettering should be clear and of adequate size to be legible after reduction. Consider
the printed page and column proportions when preparing ﬁgures.
Color ﬁgures will appear on the web at no additional charge, regardless of whether or not these illustrations
are reproduced in color in the printed version. For color reproduction in print, the corresponding author will
receive information regarding the costs from SBJ after the article has been accepted. As only one ﬁgure
caption may be used for both color and black and white versions of ﬁgures, please ensure that the ﬁgure
captions are meaningful for both versions, if applicable. Figures should be checked extremely carefully,
particularly after revisions. No changes to ﬁgures will be possible after acceptance of the manuscript. A
detailed guide on electronic artwork is available in Elsevier’s Artwork & media instructions.
The ﬁnal sizes of ﬁgures are as follows:
Maximum width for a 1-column ﬁgure: 8.5 cm
Maximum width for a 2-column ﬁgure: 17.5 cm
Maximum height: 23.2 cm
All ﬁgures must be submitted at their intended publication size.
Digital ﬁgure manipulation: The SBJ endorses the guidelines developed by the Journal of Cell Biology.
Figure legends
Figure legends should be typed double spaced at the end of the text, not on the ﬁgures, starting on a separate
page, with Arabic numerals corresponding to the ﬁgures. When symbols, arrows, numbers, or letters are used to
identify parts of the ﬁgures, identify and explain each one clearly in the legend. Do not insert symbols in theInstructions to Authors | 13

legend but describe using English terms such as “closed circles” or “open triangles”. Explain the internal scale
and identify the method of staining in photomicrographs.
Supplementary materials
JBB accepts electronic supplementary material to support and enhance your scientiﬁc research. Supplementaryﬁles oﬀer the author additional possibilities to publish supporting applications, movies, animation sequences,high-resolution images, background datasets, sound clips and more. Supplementary ﬁles supplied will be
published online alongside the electronic version of your article in Elsevier Web products, including
ScienceDirect. In order to ensure that your submitted material is directly usable, please ensure that data isprovided in one of our recommended ﬁle formats. Authors should submit the material in electronic format
together with the article and supply a concise and descriptive caption for each ﬁle. For ease of download, therecommended upper limit for the size of a single ﬁle is 10 MB. When the size of a single ﬁle is bigger than this,some users may experience problems when downloading the ﬁle. Supplementary ﬁgures and tables should belabeled as “Fig. S1” and “Table S1”. Supplementary materials may be supplied to peer reviewers but will not beedited by the journal.
For more detailed instructions please visit Elsevier’s artwork instruction pages.
Data in Brief
Authors are invited to submit a Data in Brief article alongside their research article. The Data in Brief article is
an attractive alternative to supplementary material. Data in Brief articles ensure that your data and themetadata to understand it, which is normally buried in supplementary material, is actively reviewed, curated,
formatted, indexed, given a DOI and publicly available to all upon publication. If your research article isaccepted, your Data in Brief article will also be published in the new, Open Access journal, Data in Brief. Please
use the Data in Brief template available through the below link: Data in Brief Template. The fee for Data in
Brief articles that are accepted for publication is $500, excluding tax and required by Elsevier.
For more information, please refer to Data in Brief FAQ.
Highlights
Highlights are a short collection of bullet points that convey the core ﬁndings and provide readers with a quick
textual overview of the article. These three to ﬁve bullet points describe the essence of the research (e.g.
results or conclusions) and highlight what is distinctive about it.
Highlights will be displayed in online search result lists, the contents list and in the online article, but will not(yet) appear in the article PDF ﬁle or print. Submission of highlights is optional.
For more detailed instructions please visit Elsevier’s Highlights instruction page.
AudioSlides
The journal encourages authors to create an AudioSlides presentation with their published article. AudioSlides
are brief, webinar-style presentations that are shown next to the online article on ScienceDirect. This gives
authors the opportunity to summarize their research in their own words and to help readers understand what
the paper is about. More information and examples are available at Elsevier’s AudioSlides instruction pages.
Authors will automatically receive an invitation e-mail to create an AudioSlides presentation after acceptanceof their paper.

Instructions to Authors | 14

Nomenclature of Microorganisms
The authors should use the correct names (i.e., genus and species) of all the microorganisms referenced in the
manuscript by conﬁrming with international rules of nomenclature, because the phylogenic reclassiﬁcation of
microorganisms has been reported frequently in recent years. The authors are encouraged to refer to thefollowing databases.
Prokaryotes:
International Code of Nomenclature of Prokaryotes (ICNP)
List of Prokaryotic names with Standing in Nomenclature (LPSN)
Viruses:International Code of Virus Classiﬁcation and Nomenclature (ICVCN)
Algae and fungi:
International Code of Nomenclature for algae, fungi, and plants
Protozoa:
International Code of Zoological Nomenclature
Yeasts:The Yeasts, a taxonomic study, 5th edition. Edited by C Kurtzman, JW Fell & T Boekhout. 2011,
Elsevier, Amsterdam►Page Top►JBB Top