ᚑくᄢቇℂᎿቇ⎇ⓥႎ๔
J. Fac. Sci. Tech., Seikei Univ.
Vol.46 No.㧝(2009) pp.19-24

ࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߦࠃࠆᴤ⢽ߩടᾲഠൻ㒐ᱛᴺߩ㐿⊒ᅏጊ ↱᪸ 㧘㑄↰ ᢥੱ 㧘ේ ▵ሶ Development of Prevention Method for Heat-deterioration of Oils
with Polyphenol Compounds
Yuri OKUYAMA*1 䋬Ayato KANDA*2 and Setsuko HARA *3
ABSTRACT :Prevention of heat deterioration of oils used in deep fat frying is important for the safety of frying foods.
Tocopherol, the most typical antioxidant for edible oils, is unstable at high temperature and causes
coloring of frying oils. Polyphenol compounds such as quercetin and epigalocatechin gallate have strong
antioxidative acitivities based on their radical-quenching activities. We have reported that theantioxidative activities of rooibos tea extracts which contain quercetin and lutin as major polyphenol
compounds for unsaturated oils are higher in the emulsifying system than in the bulk system. However, it
is not easy to add most polypenol compounds into oils directly because of their hydrophilic properties. In
the present study, we developed the novel method for utilization of antioxidative acitivities ofwater-soluble polypenol compound as an altanative for tocopherol by addition of these compounds intofrying foods, not into oils directly.
Keywords: polyphenol, heat-deterioration, deep fat frying, tocopherol, antioxidant, edible oils                          (Received March 16, 2009)1㧕 ᴤಽࠍ10㧑એ਄฽߻⩻ሶࠍኻ⽎ߣߒߡ㧘㉄ଔ(AV)߇

㧝㧚✜ ⺰

3ࠍ⿥߃㧘߆ߟㆊ㉄ൻ‛ଔ(PV)߇30meq/kgࠍ⿥߃ࠆ߽ߩ㧘ᴤ⢽ߪⓨ᳇ਛߩ㉄⚛ߣ෻ᔕߒߡ㉄ൻ৻ᰴ↢ᚑ‛ߢ޽ࠆ

޽ࠆ޿ߪAV߇5ࠍ⿥߃ࠆ߆㧘PV߇50 meq/kgࠍ⿥߃ࠆ߽

ࡅ࠼ࡠࡍ࡞ࠝࠠࠪ࠼ࠍ㧘㉄ൻੑᰴ↢ᚑ‛ߢ޽ࠆࠕ࡞࠺ࡅ
࠼㧘ࠤ࠻ࡦ㧘⢽⢌㉄ߥߤߩಽ⸃‛ߣฦ⒳㊀ว‛ࠍ↢ᚑߔߩߪ⽼ᄁߒߡߪߥࠄߥ޿‫ޕ‬2㧕 ᴤ⢽ߢಣℂߒߚහᏨ߼ࠎߦߟ޿ߡ㧘AV:3 એਅ㧘PV:30

ࠆ‫ޕ‬ടᾲ᧦ઙਅߦ߅޿ߡߪߎࠇࠄߩ㉄ൻ෻ᔕߪߐࠄߦଦmeq/kg એਅߢߥߌࠇ߫ߥࠄߥ޿‫ޕ‬ㅴߐࠇ㧘ഠൻ߇ᕆㅦߦㅴⴕߔࠆ‫ߡߞ߇ߚߒޕ‬㧘㘩↪ᴤ⢽

3㧕 ᑯᒰ࡮ߘ߁ߑ޿ߦߟ޿ߡ㧘ේ᧚ᢱߢ޽ࠆᴤ⢽ߩၮḰ߅ࠃ߮ᴤ⢽฽᦭㘩ຠߦ߅޿ߡ㧘ᴤ⢽ߩഠൻߪ㘑๧߿ᩕ㙃

୯߇ AV:1 એਅ㧘PV:10meq/kg એਅߢ޽ࠅ㧘200͠એਅଔߩૐਅࠍ߽ߚࠄߔߚ߼㧘ຠ⾰▤ℂ਄㧘ߘߩ㉄ൻ㒐ᱛ߇ߩ᷷ᐲߢ⺞ℂߒߡᴤ⢽㊂߇ 7㧑ᷫߞߚ႐วߦߪᣂߚߦ

ᄢ߈ߥ⺖㗴ߣߥࠆ‫ޕ‬㘩ຠⴡ↢ᴺߢߪฦ⒳ߩᴤ⢽฽᦭㘩ຠߩ቟ోၮḰߣߒߡ㧘ᴤ⢽ࠍ⵬లߔࠆ‫ߚ߹ޕ‬㧘ᴤ⢽ߩຠ⾰ഠൻߩၮḰࠍ⊒ᾍὐ:170͠㧘AV:2.5㧘ࠞ࡞ࡏ࠾࡞ଔ߇ 50 એਅߣߒ㧘ߎࠇ㧕㉄ൻഠൻᐲߦ㑐ߒߡએਅߩࠃ߁ߦቯ߼ࠄࠇߡ޿ࠆ ‫ޕ‬ࠍ⿥߃ߚ႐วߦߪᣂᴤߦ੤឵ߔࠆ‫ޕ‬*1㧦ᚑくᄢቇᄢቇ㒮Ꮏቇ⎇ⓥ⑼ඳ჻೨ᦼ⺖⒟ୃੌ↢ߘߎߢ㧘ᴤ⢽ࠍ቟ో߆ߟ᦭ലߦ೑↪ߔࠆߚ߼ߦ㧘ᴤ⢽ߩ㉄ൻഠൻࠍ㒐ᱛߔࠆᣇᴺߦߟ޿ߡߎࠇ߹ߢߦᄙߊߩᬌ

*2㧦ᚑくᄢቇℂᎿቇㇱ‛⾰↢๮ℂᎿቇ⑼ഥᚻ

⸛߇ߥߐࠇߡ߈ߚ‫ޕ‬ᴤ⢽ߩ㉄ൻഠൻ෻ᔕߪਥߦⓨ᳇ਛߩ*3㧦ᚑくᄢቇℂᎿቇㇱ‛⾰↢๮ℂᎿቇ⑼ᢎ᝼

㉄⚛ߣߩ࡜ࠫࠞ࡞ㅪ㎮෻ᔕ߅ࠃ߮ᵴᕈ㉄⚛ߦࠃࠆ㉄ൻ෻

(Professor, Dept of Materials and Life Science,e-mail: shara@st.seikei.ac.jp)ᔕߦࠃߞߡㅴⴕߔࠆ‫ޕ‬ߎࠇࠄߩ෻ᔕᯏ᭴ߦၮߠ߈㧘ᴤ⢽฽᦭㘩ຠߦ߅޿ߡߪ㧙㧙㩷એਅߦ␜ߔ⒳‫㉄ߩޘ‬ൻ㒐ᱛኻ╷߇⻠ߓࠄࠇߡ޿ࠆ1)‫ޕ‬Ԙ⸛ߔࠆߣߣ߽ߦ㧘ߘߩᕈ⁁ࠍᵴ߆ߒߚታ㓙ߩടᾲ⺞ℂߦ㉄⚛ሽ࿷㊂ߩૐᷫࠍ⋡⊛ߣߒߚ⓸⚛⟎឵㧘⌀ⓨ൮ⵝ㧘⣕߅ߌࠆ᦭ല೑↪ᴺߩ㐿⊒ࠍ⋡⊛ߣߒߚ‫ޕ‬㉄⚛೷ߩ૶↪ߥߤ㧘ԙశ㧘ᾲ㧘᡼኿✢ࠛࡀ࡞ࠡ࡯ࠍㆤᢿ
ߔࠆߚ߼ߩ൮ⵝᒻᘒߩᎿᄦ㧘಄⬿࡮಄ಓ଻ሽߥߤ㧘Ԛ㉄㧞㧚ታ㛎ᣇᴺൻଦㅴ૞↪ࠍ߽ߟࠢࡠࡠࡈࠖ࡞ߩࠃ߁ߥశჇᗵ೷ߩ♖⵾ ⹜ᢱߣ⹜⮎ߦࠃࠆ㒰෰㧘ԛ㊄ዻߩ⸅ᇦ૞↪ࠍᛥ೙ߔࠆߚ߼ߩ㊄ዻਇᵴᕈ೷ߩ૶↪㧘Ԝ࡜ࠫࠞ࡞ㅪ㎮෻ᔕࠍ஗ᱛߔࠆ㉄ൻ㒐ᱛࡐ࡝ࡈࠚࡁ࡯࡞ࠍ฽߻⹜ᢱߣߒߡ RTE7㧕㧘Pro7㧕ࠍ㧘ࡐ೷ߩ૶↪‫ߩࠄࠇߎޕ‬ᣇᴺߪߘࠇߙࠇ⚵ߺวߖߡታ㓙ߩ㘩࡝ࡈࠚࡁ࡯࡞ᮡḰ‛⾰ߣߒߡ㧘Ru㧘Qu㧘ࠛࡇࠟࡠࠞ࠹ࠠ

ຠߦ߅޿ߡ೑↪ߐࠇߡ޿ࠆ߇㧘ߎࠇࠄߩਛߢᦨ߽ലᨐ⊛ࡦࠟ࡟࡯࠻(EGCG)㧘ᴚ㘩ሶ㉄ࡊࡠࡇ࡞(PG)ࠍ↪޿ߚ‫ޕ‬ടߢ޽ࠆߩ߇Ԝߩ㉄ൻ㒐ᱛ೷ߩ೑↪ߢ޽ࠆ‫ޕ‬ᾲ⹜㛎ߩ⹜ᢱᴤ⢽ߣߒߡ㧘ਥ᭴ᚑ⢽⢌㉄߇࡝ࡁ࡯࡞㉄ߢ ㉄ൻ㒐ᱛ೷ߣߒߡ⃻࿷ᦨ߽ᄙߊ೑↪ߐࠇߡ޿ࠆ߽ߩߪ

޽ࠆᄢ⼺ᴤ(ᣣᷡࠝࠗ࡝ࠝࠣ࡞࡯ࡊࢃ⵾)ߣߎࠇࠍࠞ࡜ࡓ

߶ߣࠎߤߩᬀ‛ᴤߦ฽߹ࠇߡ޿ࠆᄤὼᚑಽߢ޽ࠆ࠻ࠦࡈࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ߦࠃࠅ♖⵾ߒߚ♖⵾ᄢ⼺ᴤߩ㧞⒳ࠍ

1)

ࠚࡠ࡯࡞㧔Toc㧕ߢ޽ࠆ ‫ޕ‬Toc ߪࡈࠚࡁ࡯࡞ᕈ᳓㉄ၮ߆↪޿ߚ‫ޕ‬ࠄ㧘⢽⾰ߩ㉄ൻߦࠃߞߡ↢ᚑߒߚ⢽⾰ࡍ࡞ࠝࠠࠪ࡜ࠫࠞ
࡞㧔LOO㨯㧕߳᳓⚛࡜ࠫࠞ࡞ࠍଏਈߒ㧘⥄ࠄ߇቟ቯߥ࡜ ࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩቯ㊂  ࠫࠞ࡞ߣߥࠆߎߣߦࠃߞߡ࡜ࠫࠞ࡞ㅪ㎮෻ᔕࠍ஗ᱛߔࠆ ࡈࠚࡁ࡯࡞ൻว‛ᬌ಴⹜⮎㧔Folin-Ciocalteu ⹜⮎㧕ࠍ૞↪ࠍ␜ߔ‫ޕ‬ㅢᏱ㧘ᴤ⢽ߦ 500-1000ppm ࠍᷝടߔࠆߣᴤ↪޿ߡ㧘RTE㧘Ru㧘Qu ߅ࠃ߮ Pro ߩࡐ࡝ࡈࠚࡁ࡯࡞฽᦭⢽ߩ㉄ൻ߇ലᨐ⊛ߦᛥ೙ߐࠇࠆ‫ߚ߹ޕ‬㧘ࠕࠬࠦ࡞ࡆࡦ㉄㊂ࠍๆశᐲᴺ(760nm)ߦࠃࠅ᷹ቯߒߚ‫ޕ‬ቯ㊂ߦ㓙ߒߡߪ㧘߿࡝ࡦ⢽⾰ߩ౒ሽߦࠃࠅ Toc ߩ㉄ൻ㒐ᱛലᨐߪ⋧ਸ਼⊛ߦ੍ ߼ ᮡ Ḱ ‛ ⾰ ߣ ߒ ߡ EGCG ࠍ ↪ ޿ ߡ ᬌ ㊂ ✢ะ਄ߔࠆߎߣ߽⍮ࠄࠇߡ޿ࠆ

㧔y=0.0122x+0.1027, R2=0.9836㧕ࠍ૞ᚑߒ㧘ߔߴߡߩࡐ࡝2,3㧕

‫ߒ߆ߒޕ‬㧘Toc ߪㆊ೾ߦᷝടߐࠇߚ႐วߦߪ㉄ൻଦㅴലᨐࠍ␜ߔߎߣ߇޽ࠆߚ߼㧘ࡈࠚࡁ࡯࡞ൻว‛㊂ࠍ EGCG ㊂ߦ឵▚ߒߡ᳞߼ߚ‫ޕ‬

ᷝട㊂ߦ⇐ᗧߔࠆߎߣ߇ᔅⷐߢ޽ࠆ‫ߚ߹ޕ‬㧘৻⥸⊛ߥട
ᾲ⺞ℂ᷷ᐲߢ޽ࠆ 160͠એ਄ߩ㜞᷷ߦߥࠆߣᕆㅦߦಽ⸃ ࡜ࠫࠞ࡞ᶖ෰⢻ߩ᷹ቯ 㧕ߒ㧘㉄ൻ㒐ᱛലᨐ߇ૐਅߔࠆߎߣ㧘ߐࠄߦ Toc ߩ㉄ൻ↢1.1-diphenyl-2-picrylhydrazil (DPPH)ࠛ࠲ࡁ࡯࡞ṁᶧࠍᚑ‛ߦࠃࠆᴤ⢽ߩ⌕⦡⃻⽎߇⷗ࠄࠇࠆߎߣߥߤ߇ႎ๔ߐ↪޿ߚᣇᴺߦࠃࠅ㧘ฦࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩ࡜ࠫࠞ࡞ࠇ㧘ᴤ⢽ߩടᾲഠൻࠍലᨐ⊛ߦ㒐ᱛߢ߈ࠆ㧘Toc ߦઍࠊᶖ෰⢻ࠍๆశᐲᴺ(517nm)ߦࠃࠅ⹏ଔߒߚ‫߅ߥޕ‬㧘ฦࡐ࡝ࠆᣂߚߥ㉄ൻ㒐ᱛ೷ߩ㐿⊒߇ᦸ߹ࠇߡ޿ࠆ‫ޕ‬ࡈࠚࡁ࡯࡞ߩ࡜ࠫࠞ࡞ᶖ෰⢻ߪ Toc ߩᶖ෰⢻ࠍ 1.00 ߣߔ ৻ᣇ㧘⨥⪲ߦ฽߹ࠇߡ޿ࠆࠞ࠹ࠠࡦ㘃ߥߤ㧘ᄤὼ‛ߦࠆ⋧ኻ୯ߢ␜ߒߚ‫ޕ‬฽߹ࠇࠆࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩ↢ℂᯏ⢻߇ᵈ⋡ߐࠇ㧘ߎࠇ߹ߢߦᄙߊߩࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩ࡜ࠫࠞ࡞ᶖ෰⢻ߦ㑐ߔࠆ⎇ⓥ߇ႎ๔ߐࠇߡ޿ࠆ

 %&/ ⹜㛎ߦࠃࠆ㉄ൻ㒐ᱛലᨐߩ᷹ቯ
Toc ࠍ㒰෰ߒߚ♖⵾ᄢ⼺ᴤߦ RTE㧘Pro㧘Ru㧘Qu㧘EGCG㧘

4-6㧕

‫ޕ‬

╩⠪ࠄߪߎࠇ߹ߢߦධࠕࡈ࡝ࠞ↥ߩ࡞ࠗࡏࠬ࠹ࠖ᛽಴

PG ࠍ Toc500ppm ߩ࡜ࠫࠞ࡞ᶖ෰⢻⋧ᒰ㊂ᷝടߒ㧘150͠

‛(RTE)ߦ฽߹ࠇࠆࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛㧘߅ࠃ߮㧞⒳ߩ

ߣ 180͠ߢ㉄ൻ቟ቯᕈ(CDM)⹜㛎ࠍⴕߞߚ‫ߚ߹ޕ‬㧘ടᾲ߱ߤ߁⒳ሶ᛽಴‛ߢ޽ࠆࡊࡠࠪࠫࡦ㧔Pro㧕ߣࠣ࡜ࡆࡁ࡯

ᴤߩࡐ࡝ࡈࠚࡁ࡯࡞ᱷሽ₸ߩ⚻ᤨᄌൻࠍ⺞ߴߚ‫ޕ‬࡞㧔Gra㧕ߩ㉄ൻ㒐ᱛലᨐߦߟ޿ߡᬌ⸛ߒ㧘RTE ߩਥߚࠆࡐ࡝ࡈࠚࡁ࡯࡞ᚑಽߪ࡞࠴ࡦ(Ru)ߣࠤ࡞࠮࠴ࡦ(Qu)ߢ

 ࡈ࡜ࠗടᾲ⹜㛎ߦࠃࠆ㉄ൻ㒐ᱛലᨐߩ᷹ቯ޽ࠅ㧘ࡐ࡝ࡈࠚࡁ࡯࡞㊂ᒰࠅߩ࡜ࠫࠞ࡞ᶖ෰⢻ߪ Toc ߦᲧセߒߡ㜞޿ߎߣࠍႎ๔ߒߡ޿ࠆ

7㧕ታ㓙ߩടᾲ⺞ℂࠍ⠨ᘦߒߡ㧘឴ߍ⒳ࠍ឴ߍ㧘ടᾲ㉄ൻ‫ߦࠄߐޕ‬㧘࡝ࡁ࡯࡞ߦട߃ߡട᳓ಽ⸃߽⿠ߎࠆ᧦ઙਅߦࡐ࡝ࡈࠚࡁ࡯࡞ൻว㉄ࡔ࠴࡞ߦኻߔࠆ㉄ൻ㒐ᱛലᨐࠍ 30͠ᥧᚲਅߢߩ㕖᳓‛ߩ㉄ൻ㒐ᱛ⢻ߦߟ޿ߡᬌ⸛ߒߚ‫ߜࠊߥߔޕ‬㧘Tocࠍ฽߹♽⹜㛎߅ࠃ߮੃ൻ♽⹜㛎ߢᬌ⸛ߒ㧘RTE ߪ੃ൻ♽ߢ㗼⪺ߥ㉄ൻ㒐ᱛലᨐࠍ␜ߒ㧘․ߦ᳓ጀߦ⊒↢ߔࠆ࡜ࠫࠞ࡞
ߩ᝝ᝒߦ᦭ലߢ޽ࠆߎߣࠍ᣿ࠄ߆ߦߒߡ޿ࠆ 8㧕‫ޕ‬ߥ޿♖⵾ᄢ⼺ᴤ100㨓ࠍၮ⾰ߣߒߡ㧘Ԙၮ⾰ (ࠦࡦ࠻ࡠ࡯࡞)㧘ԙ ၮ⾰ + Toc 500ppm㧘Ԛ ၮ⾰ + RTE 769.2ppm(࡜ࠫࠞ࡞ᶖ෰⢻ߣߒߡToc500ppm⋧ᒰ)㧘ԛ ၮ⾰ + Proߎࠇࠄߩ⚿ᨐߦၮߠߊߣ㧘ᴤ⢽ߩടᾲഠൻࠍ RTE ߦࠃ

161.3ppm(࡜ࠫࠞ࡞ᶖ෰⢻ߣߒߡToc500ppm⋧ᒰ)ߩ4⒳ߩ

ࠅ㒐ᱛߔࠆߎߣ߇ᦼᓙߐࠇࠆ‫⎇ᧄޕ‬ⓥߢߪᴤ⢽ߩടᾲഠ⹜ᢱᴤࠍ⺞⵾ߒߚ‫ޕ‬឴ߍ⒳ߦߪᄤ߲ࠄ☳(ᤘ๺↥ᬺࢃ⵾㧘ൻߦኻߔࠆࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩ㒐ᱛലᨐߦߟ޿ߡᬌ

એᓟ㧘☳ߣ⴫⸥) 4 gࠍࠗࠝࡦ੤឵᳓㧔એᓟ㧘᳓ߣ⴫⸥㧕

㧙㧙

㩷

6 gߢṁ߆ߒߚ߽ߩࠍ↪޿ߚ‫⹜ޕ‬ᢱᴤࠍ180͠ߢ24 hടᾲߒ㧘

2 hߏߣߦ឴ߍ⒳ࠍᢙಽ㑆឴ߍ㧘4 hߏߣߦ⹜ᢱᴤࠍណขߒ㧘ᭂᕈൻว‛㧔PC㧕㊂ࠍ⚻ᤨ⊛ߦ᷹ቯߒߚ‫ޕ‬
߹ߚ㧘RTEࠍ឴ߍ⒳ߦᷙߗߡ↪޿ߚ♽ߦߟ޿ߡߪ㧘ᄢ⼺ᴤ(Toc 508ppm฽᦭)100㨓ࠍ⹜ᢱᴤߣߒߡ㧘180͠ߢว⸘24 hടᾲߒߚ‫ޕ‬឴ߍ⒳ߦߪએਅߩ5⒳ࠍ↪޿㧘RTEᷝട࡯࡞ൻว‛ߩ࡜ࠫࠞ࡞ᶖ෰⢻ߪ㧘 ߩࡐ࡝ࡈࠚࡁ࡯࡞฽᦭₸ࠍ↪޿ߡࡐ࡝ࡈࠚࡁ࡯࡞㊂޽ߚࠅߦ឵▚ߒ㧘Toc
ࠍ 1.00 ߣߒߚ⋧ኻ୯ߣߒߡ⴫  ߦ␜ߒߚ‫ޕ‬RTE ߪ㧘ࡐ࡝ࡈࠚࡁ࡯࡞޽ߚࠅߢߪ Toc ߩ⚂ 2 ୚ߩᶖ෰⢻ࠍ␜ߒ㧘߹ߚ㧘RTE એᄖߩࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߪ Toc ߩ 2㨪4 ୚ߩఝࠇߚ࡜ࠫࠞ࡞ᶖ෰⢻ࠍᜬߟߎߣ߇⏕⹺ߐࠇߚ‫ޕ‬㊂ߣ⺞ℂ㑆㓒ߦߟ޿ߡᲧセᬌ⸛ߒߚ‫ޕ‬Ԙ㨇☳4g+᳓6g+RTE0.2g]12࿁ ✚RTE㊂㧦2.4g

⴫  ࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩ࡜ࠫࠞ࡞ᶖ෰⢻ԙ㨇☳4g+᳓6g+RTE0.3g]12࿁ ✚RTE㊂㧦3.6g

ࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛Ԛ㨇☳4g+᳓6g+RTE0.4g]12࿁ ✚RTE㊂㧦4.8g

Toc

DPPH ࡜ࠫࠞ࡞ᶖ෰⢻1.00RTEԛ㨇☳2g+᳓3g+RTE0.1g]24࿁ ✚RTE㊂㧦2.4g
Ԝ㨇☳1g+᳓1.5g+RTE0.05g]48࿁ ✚RTE㊂㧦2.4g
ߥ߅㧘⺞ℂ㑆㓒ߪԘ-Ԛߢߪ2 h㧘ԛߢߪ1㨔㧘Ԝߢߪ0.5

1.83

Pro

3.12

Qu

3.74

Ru㨔ߏߣߣߒߚ‫ޕ‬ടᾲ4 hߏߣߦ⹜ᢱᴤࠍណขߒ㧘PC㊂㧘
AV㧘ࠕ࠾ࠪࠫࡦଔ㧔AnV㧕
㧘㊀ว‛㊂㧘Tocᱷሽ₸㧘ࡐ࡝

2.57

EGCG

2.82

PG

ࡈࠚࡁ࡯࡞㊂ࠍ⚻ᤨ⊛ߦ᷹ቯߒߚ‫ޕ‬

2.67 ឴ߍ⒳ߩ⥄േ㉄ൻ⹜㛎ᄢ⼺ᴤ(Toc 508ppm฽᦭)ࠍ180͠ߢടᾲߒ㧘3⒳ߩ឴ߍ

⒳㧔RTEήᷝട㧔☳4g +᳓6g㧕㧘RTEᷝടԘ㧔☳4g+᳓

6g+RTE0.1g㧕
㧘RTEᷝടԙ㧔☳4g+᳓6g+ RTE0.2g㧕ࠍ឴ߍ㧘
឴ߍ⒳߆ࠄࠢࡠࡠࡎ࡞ࡓ࡮ࡔ࠲ࡁ࡯࡞(2:1)ࠍ↪޿ߡ᛽಴ߒߚᴤ⢽ࠍ30͠ᥧᚲߢ⥄േ㉄ൻߒ㧘PV9㧕ࠍ⚻ᤨ⊛ߦ᷹ቯ %&/ ⹜㛎ߦࠃࠅ᷹ቯߒߚ㉄ൻ㒐ᱛലᨐ ࿑  ߦ 150͠ߣ 180͠ߩ CDM ⹜㛎ߦ߅ߌࠆࡐ࡝ࡈࠚࡁ
࡯࡞ᷝട⹜ᢱᴤߩ⺃ዉᦼࠍ␜ߒߚ‫ޕ‬࿑  ߩฦ㉄ൻ㒐ᱛ೷ߩലᨐࠍᲧセߔࠆߣ 150͠ߢߪ㧘Qu㧪TocѳRTEѳProѳ
RuѳEGCGѳPG㧘180͠ߢߪ㧘Pro㧪RTE㧪Qu㧪Toc㧪Ruᵏᵓᵎḥߒߚ‫ޕ‬ᵎᵌᵒᵓᵎᵌᵓᵑ
ᵎᵌᵒᵗᵎᵌᵓᵎᵎᵌᵓᵏἅὅἚἿὊἽᵲᶍᶁ ㉄ൻഠൻᐲߩ᷹ቯ
⢽⾰ߩ㉄ൻഠൻߩᜰᮡߣߒߡ↪޿ߚPV9㧕㧘౒ᓎࠫࠛࡦᵰᵲᵣᵮᶐᶍ฽᦭₸㧘AnV㧘AVߪᣣᧄᴤൻቇળ✬ߩၮḰᴤ⢽⹜㛎ಽᨆᵰᶓߩᣇᴺߦၮߠ޿ߡ᷹ቯߒߚ‫ߚ߹ޕ‬㧘PC㊂ߪHaraᵯᶓ

10㧕ᴺ⸥タᵎᵌᵕᵒࠄߩᣇᴺߦᓥߞߞߡ᷹ቯߒߚ11㧕‫ޕ‬㊀ว‛㊂ߪᣣᧄಽశࢃ

ᵎᵌᵒᵗ
ᵎᵌᵓᵔ

ᵣᵥᵡᵥ

⵾ࠥ࡞ᶐㅘࠢࡠࡑ࠻ࠣ࡜ࡈPU-2080 Plusဳߦห␠⵾ࠞ࡜

ᵮᵥ

ࡓKF-802.5 C 508007 (8.0mm×300mm)ߣRI-2031 Plusဳᬌ

ᵎᵎᵌᵐ

ᵎᵌᵒ

ᵎᵌᵔ

ᵎᵌᵖᛔ‫ݰ‬஖ᵆᶆᵇ಴ེࠍធ⛯ߒ㧘࠹࠻࡜ࡅ࠼ࡠࡈ࡜ࡦࠍᵹㅦ0.7mL/minߢᵹߒߡቯ㊂ߒߚ12㧕‫ޕ‬ᵏᵖᵎḥᵎᵌᵏᵖἅὅἚἿὊἽᵎᵌᵐᵕᵲᶍᶁ㧟 ⚿ᨐߣ⠨ኤᵎᵌᵒᵕ

ᵰᵲᵣᵮᶐᶍᵰᶓᵎᵌᵐᵑ ࡐ࡝ࡈࠚࡁ࡯࡞฽᦭₸
RTE㧘Pro㧘Ru㧘Qu㧘
PG ߩࡐ࡝ࡈࠚࡁ࡯࡞฽᦭₸ࠍ EGCGᵣᵥᵡᵥ

឵▚ߢ▚಴ߒߚ⚿ᨐ㧘RTE㧦35.4%㧘Pro㧦99.3%㧘Ru㧦ᵮᵥ

ᵯᶓ48.0%㧘Qu㧦92.6%㧘PG㧦128.2%ߢ޽ߞߚ‫ޕ‬ᵎᵌᵑᵗᵎᵌᵐᵐᵎᵌᵐᵑᵎᵎᵌᵐᵎᵌᵒᵎᵌᵔᵎᵌᵖᛔ‫ݰ‬஖ᵆᶆᵇ &22* ࡜ࠫࠞ࡞ᶖ෰⢻
RTE㧘Pro㧘Ru㧘Qu㧘EGCG㧘PG ߩ࡜ࠫࠞ࡞ᶖ෰⢻ࠍ㧘

DPPH ࡜ࠫࠞ࡞ࠍ↪޿ߡ⹏ଔߒߚ‫߅ߥޕ‬㧘ฦࡐ࡝ࡈࠚࡁ࿑  %&/ ⹜㛎ߦ߅ߌࠆฦࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߩ㉄ൻ  㒐ᱛലᨐߩᲧセ㧙㧙㩷Toc ߢߪ PC ㊂ߩ਄᣹߇ᛥ೙ߐࠇ㧘㉄ൻ㒐ᱛലᨐ߇⷗ࠄ

ѳEGCGѳPG ߢ޽ߞߚ߇㧘޿ߕࠇߩ㉄ൻ㒐ᱛ೷߽ടᾲ᷷ᐲߩ਄᣹ߦ઻ߞߡ⺃ዉᦼߪ⍴❗ߐࠇߚ‫ߚ߹ޕ‬㧘150͠ߩട

ࠇߚߩߦኻߒ㧘RTE ߣ Pro ߢߪࠦࡦ࠻ࡠ࡯࡞ߣ߶ߣࠎߤ

ᾲ᷷ᐲߢߪ RTE ᷝട♽߅ࠃ߮ Pro ᷝട♽ߪ Toc ᷝട♽ߣᏅ߇ߥߊ㧘㉄ൻ㒐ᱛലᨐ߇ⷰኤߐࠇߥ߆ߞߚ‫ߪࠇߎޕ‬㧘ਔ♽ߣ߽ Toc ᷝട♽ߩ⚂ 2 ୚ߩ⺃ዉᦼࠍ␜ߒߚ‫⚿ߩߎޕ‬ࠆߚ߼㧘ᴤ⢽ߦߪṁߌߥ޿⁁ᘒߢ⹜ᢱᴤਛߦሽ࿷ߒ㧘᳓߶߷ห╬ߩ㉄ൻ቟ቯᕈࠍ␜ߒߚ߇㧘180͠ߩടᾲ᷷ᐲߢߪ㧘
ᨐࠃࠅ㧘RTE ᷝട♽߅ࠃ߮ Pro ᷝട♽ߪ᷷ᐲߩᓇ㗀ࠍฃToc ߇ᴤṁᕈߢ޽ࠆߩߦኻߒ㧘RTE ߣ Pro ߪ᳓ṁᕈߢ޽ࠍ฽߻឴ߍ⒳ࠍᛩ౉ߔࠆߣߘߩⷫ᳓ᕈߩߚ߼឴ߍ⒳ߦๆ

ߌߦߊߊ㧘㜞᷷ߦ߅޿ߡߪ㉄ൻ቟ቯᕈ߇ Toc ᷝട♽ࠃࠅ⌕ߐࠇ㧘឴ߍ⒳ߣߣ߽ߦ♽ᄖ߳ขࠅ㒰߆ࠇߚߚ߼ߣ⠨߃ఝࠇߡ޿ࠆߎߣ߇್᣿ߒߚ‫ޕ‬ࠄࠇߚ‫ޕ‬ ߹ߚ㧘180͠ടᾲᤨߩฦ⹜ᢱਛߩࡐ࡝ࡈࠚࡁ࡯࡞ᱷሽ₸ߣ Toc ᱷሽ₸ߪ࿑  ߦ␜ߒߚࠃ߁ߦ㧘RTE ߩࡐ࡝ࡈࠚࡁ

ᰴߦ឴ߍ⒳ߦ RTE ࠍᷝടߒߚ 5 ⹜ᢱࠍ↪޿ߡ㧘RTE ߩ㉄ൻ㒐ᱛലᨐߦߟ޿ߡᬌ⸛ߒ㧘ߘߩ⚿ᨐࠍ࿑  ߦ␜ߒߚ‫ޕ‬࡯࡞ᱷሽ₸ߪ Toc ߿ߘߩઁߩ㉄ൻ㒐ᱛ೷ߩᱷሽ₸ߦᲧߴߡ㜞ߊ㧘⠴ᾲᕈ߇⹺߼ࠄࠇߚߚ߼㧘ടᾲᴤߦኻߔࠆ㉄ൻ㪊㪋㪅㪐㪩㪫㪜ήᷝട㒐ᱛലᨐ߇㜞޿ߎߣ߇ផ᷹ߐࠇߚ‫ޕ‬㪉㪇㪅㪋㪩㪫㪜ᷝട㽲㪐㪅㪈㪈㪇㪅㪋㪈㪊㪅㪇㪩㪫㪜ᷝട㽳㪈㪇㪇ᱷሽ₸䋨䋦䋩㪩㪫㪜ᷝട㽴㪏㪇

㪩㪫㪜ᷝട㽵

㪍㪇㪩㪫㪜ᷝട㽶

㪉㪌㪅㪇
㪇㪅㪇

㪈㪇㪅㪇

㪉㪇㪅㪇

㪊㪇㪅㪇

㪋㪇㪅㪇

㪋㪇

㪧㪚㊂䋨䋦䋩
㪉㪇㪇
㪇

㪉㪇

㪋㪇
㪍㪇
ടᾲᤨ㑆㩿㫄㫀㫅䋩㪏㪇 Ԙ㨇☳ 4g+᳓ 6g+RTE0.2g]12 ࿁ ✚ RTE ㊂㧦2.4g

ԙ㨇☳ 4g+᳓ 6g+RTE0.3g]12 ࿁ ✚ RTE ㊂㧦3.6g

Ԛ㨇☳ 4g+᳓ 6g+RTE0.4g]12 ࿁ ✚ RTE ㊂㧦4.8g

㪫㫆㪺㪩㪫㪜㪧㫉㫆

㪨㫌

㪜㪞㪚㪞

㪧㪞

ԛ㨇☳ 2g+᳓ 3g+RTE0.1g]24 ࿁ ✚ RTE ㊂㧦2.4g

㪩㫌Ԝ㨇☳ 1g+᳓ 1.5g+RTE0.05g]48 ࿁ ✚ RTE ㊂㧦2.4g

  ࿑  ͠ടᾲߦ߅ߌࠆᱷሽ₸ߩᲧセ   ࿑  ឴ߍ⒳߳ᷝടߒߚ 46' ߩ㉄ൻ㒐ᱛലᨐ࿑  ߆ࠄ RTE ήᷝട♽ߦᲧセߒߡߔߴߡߩ RTE ᷝട ࡈ࡜ࠗടᾲ⹜㛎ߦࠃࠆ㉄ൻ㒐ᱛലᨐߩ᷹ቯ
߹ߕ㧘RTE ߅ࠃ߮ Pro ࠍᴤ⢽ߦᷝടߒߡടᾲߒ㧘PC ㊂
ߩ⚻ᤨ⊛ᄌൻࠍ࿑  ߦ␜ߒߚ‫ߦ࡞࡯ࡠ࠻ࡦࠦޕ‬Ყセߒߡ♽ߢ PC ㊂ߩ਄᣹߇ᛥ೙ߐࠇ㧘ᴤ⢽ߦ⋥ធᷝടߒߚ႐ว

ߦߪ⹺߼ࠄࠇߥ߆ߞߚ RTE ߩ㉄ൻ㒐ᱛലᨐ߇㧘ᢿ⛯⊛ߦ឴ߍ⒳ߣߣ߽ߦᷝടߔࠆߎߣߦࠃߞߡ⊒ើߐࠇࠆߎߣ߇㪏㪇್᣿ߒߚ‫ߚ߹ޕ‬㧘ᷝട㊂ߦߟ޿ߡߪԘ㨪ԚߩᲧセ߆ࠄࡈ㪎㪇࡜ࠗᴤ 100㨓ߦኻߒ㧘RTE ࠍ✚㊂ߣߒߡԙ3.6㨓ᷝടߒߚ㪍㪇㪧㪚㊂䋨䋦䋩♽߇ᦨ߽ലᨐ⊛ߢ޽ߞߚ‫ߚ߹ޕ‬㧘ᷝട㑆㓒ߩᓇ㗀ߦߟ޿㪌㪇ߡߪԘ㧘ԛ㧘ԜߩᲧセ߆ࠄ RTE ࠍ 1㨔ߏߣߦ឴ߍ⒳ߦᷙ

㪋㪇㪊㪇วߒߚԛ߇᦭ലߢ޽ߞߚ‫ߒ߆ߒޕ‬㧘ԙߣԛߩࡈ࡜ࠗᴤߩ
ࡐ࡝ࡈࠚࡁ࡯࡞฽᦭㊂ߪ޿ߔࠇ߽ 5-10ppm ߢ޽ߞߚߎߣ㪉㪇߆ࠄ㧘RTE ᷝട㊂ࠍჇ߿ߔࠃࠅ߽ᷝട㗫ᐲࠍ㜞߼ࠆߎߣ㪈㪇߇ࠃࠅ᦭ലߢ޽ࠆߣ⠨ኤߒߚ‫߅ߥޕ‬㧘Ԝߦ߅޿ߡߪ 1 ࿁㪇㪇㪈㪇㪉㪇㪊㪇ടᾲᤨ㑆䋨䌨䋩䉮䊮䊃䊨䊷䊦㪫㫆㪺

ߩᷝട㊂߇ዋߥߊ㧘⹜ᢱᴤߩࡐ࡝ࡈࠚࡁ࡯࡞฽᦭㊂߇਄᣹ߒߥ߆ߞߚߚ߼ߣ⠨߃ࠄࠇߚ‫ޕ‬㪧㫉㫆㪩㪫㪜࿑  ࡈ࡜ࠗᴤߦᷝടߒߚ 46' ߩ㉄ൻ㒐ᱛലᨐ

㧙㧙

㩷

 ឴ߍ⒳ߩ⥄േ㉄ൻ⹜㛎ߞߚ‫ߜࠊߥߔޕ‬㧘ࡈ࡜ࠗടᾲ⹜㛎Ԙߢߪ㧘♖⵾ᄢ⼺ᴤߦᄢ⼺ᴤࠍ 180͠ߢടᾲߒ㧘3 ⒳ߩ឴ߍ⒳㧔RTE ήᷝട㧦࡜ࠫࠞ࡞ᶖ෰⢻ߣߒߡ Toc 500 ppm ߦ⋧ᒰ㊂ᷝടߒߚ߽

ᷝടԙ㧔☳ 4g+᳓ 6g+ RTE0.2g㧕ࠍ឴ߍ㧘឴ߍ⒳߆ࠄࠢࡠ

ᴤߣߣ߽ߦ 180͠ߢ 24 h ടᾲߒ㧘ᄤ߲ࠄ☳ 4 g ࠍࠗࠝࡦ☳ 4g +᳓ 6g㧕㧘RTE ᷝടԘ㧔☳ 4g+᳓ 6g+RTE0.1g㧕㧘RTEࡠࡎ࡞ࡓ࡮ࡔ࠲ࡁ࡯࡞(2:1)ࠍ↪޿ߡ᛽಴ߒߚᴤ⢽ࠍ 30͠ᥧᚲߢ⥄േ㉄ൻߒ㧘PV ࠍ⚻ᤨ⊛ߦ᷹ቯߒߚ‫⚿ߩߘޕ‬ᨐ㧘࿑  ߦ␜ߒߚࠃ߁ߦ㧘RTE ήᷝടߩ឴ߍ⒳߆ࠄߩ᛽಴ᴤߦᲧセߒߡ㧘RTE ᷝട឴ߍ⒳߆ࠄߩ᛽಴ᴤߪ PV ߩ਄᣹ߩࠍ㧘Toc ࠍ฽߹ߥ޿♖⵾ᄢ⼺ᴤࠍ Toc 500 ppm ᷝടᄢ⼺੤឵᳓ 6 g ߢṁ߆ߒߚ឴ߍ⒳ࠍ 2 h ߏߣߦ឴ߍߡ⹜ᢱᴤߩ

㉄ൻഠൻ᜼േࠍᬌ⸛ߒߚ߇㧘RTE ߣ Pro ߩ޿ߕࠇߦ߅޿ߡ߽㉄ൻ㒐ᱛലᨐߪ⹺߼ࠄࠇߥ߆ߞߚ‫ ߪࠇߎޕ‬RTE ߣPro ߇ᴤߦṁߌߕߦ⹜ᢱᴤਛߦಽᢔߒߚ⁁ᘒߢ޽ࠆߚ߼㧘߇ᛥ೙ߐࠇ㧘RTE ߩ㉄ൻ㒐ᱛലᨐ߇⊒ើߐࠇߡ޿ࠆߎߣ

ࡈ࡜ࠗᤨߦ឴ߍ⒳ߦๆ⌕ߐࠇ㧘♽ᄖߦ឴ߍ⒳ߣߣ߽ߦข

߇ⷰኤߐࠇߚ‫⚿ߩߎޕ‬ᨐ߆ࠄ㧘RTE ࠍᷝടߒߚ឴ߍ⒳ߪ

ࠅ㒰߆ࠇߡߒ߹ߞߚߚ߼ߣ⠨߃ࠄࠇߚ‫ޕ‬㉄ൻ቟ቯᕈ߇ะ਄ߔࠆߎߣ㧘ߐࠄߦ឴ߍ⒳߆ࠄߩ᛽಴ᴤ

ߘߎߢ㧘ࡐ࡝ࡈࠚࡁ࡯࡞ࠍ឴ߍ⒳ߦᷙߗߡࡈ࡜ࠗടᾲ

ߪࡈ࡜ࠗᴤߣหߓᕈ⁁ࠍ߽ߟߎߣ߆ࠄ㧘ࡈ࡜ࠗᴤߩടᾲ

⹜㛎ԙࠍⴕߞߚ‫⹜ޕ‬ᢱᴤߣߒߡ Toc ࠍ 500ppm ⒟ᐲ฽᦭ᓟߩ଻ሽ቟ቯᕈ߽ะ਄ߒߡ޿ࠆߎߣ߽␜ໂߐࠇߚ‫ޕ‬ߔࠆᄢ⼺ᴤࠍ㧘ࠦࡦ࠻ࡠ࡯࡞ߦߪ♖⵾ᄢ⼺ᴤࠍ↪޿㧘RTEߩ✚ᷝട㊂ࠍ⹜ᢱᴤ 100㨓ߦኻߒߡ 2.4㧘3.6㧘4.8㨓㧘߹ߚ㧘឴ߍ⒳ߩ⺞ℂ㑆㓒ࠍ 2 h㧘1 h㧘0.5 h ߣߒߡ⹜㛎ߒߚ‫ޕ‬㪎㪇ߘߩ⚿ᨐ㧘RTE ࠍ឴ߍ⒳ߦᷙߗߡ↪޿ߚ႐วߦߪ RTE ߩ㪧㪭㩿㫄㪼㫈㪆㫂㪾㪀㪍㪇㉄ൻ㒐ᱛലᨐ߇ⷰኤߐࠇ㧘ᦨㆡߥ RTE ᷝട㊂ߪ⹜ᢱᴤ㪌㪇

100㨓ߦኻߒ㧘3.6g ߢ޽ߞߚ‫߅ߥޕ‬㧘ߎߩታ㛎ߦ߅޿ߡࡈ㪋㪇࡜ࠗᴤਛߩࡐ࡝ࡈࠚࡁ࡯࡞㊂ߪ⹜㛎ਛ 5㨪10 ppm ߢ৻ቯ㪊㪇㪉㪇ߢ޽ߞߚߩߢ㧘⺞ℂਛߩ឴ߍ⒳߆ࠄߩࡐ࡝ࡈࠚࡁ࡯࡞ṁ㪈㪇

಴㊂ߣಽ⸃࡮㊀วߥߤߦࠃࠆᷫዋ㊂߇ဋⴧߒߡ޿ࠆߣ⠨㪇߃ࠄࠇߚ‫ߦࠄߐޕ‬㧘⺞ℂ㑆㓒ߦߟ޿ߡߪ㧘1h ߇ᦨㆡߢ޽㪇㪉㪇㪇㪋㪇㪇

㪍㪇㪇ࠅ㧘ߎࠇߪ឴ߍ⒳ߦᷙߗߚࡐ࡝ࡈࠚࡁ࡯࡞ߩᛩ౉ߔࠆ㗫଻ሽᤨ㑆䋨䌨䋩㪩㪫㪜ήᷝട
㪩㪫㪜ᷝട㽳

㪩㪫㪜ᷝട㽲

ᐲ߇ 2㨔ࠃࠅ 1㨔ߩ߶߁߇㧘ࡈ࡜ࠗᴤਛߩࡐ࡝ࡈࠚࡁ࡯࡞㊂߇ᄙߊ଻ߚࠇࠆߚ߼ߣ್ᢿߐࠇߚ‫ޕ‬
ᰴߦ㧘RTE ࠍᷝടߒߡ⺞ℂߒߚ឴ߍ⒳߆ࠄᴤ⢽ࠍ᛽಴ߒ㧘30͠ᥧᚲߩᕡ᷷ᮏਛߢ⥄േ㉄ൻ⹜㛎ࠍⴕ޿㧘឴ߍ⒳࿑  46' ᷝട឴ߍ⒳߆ࠄߩ᛽಴ᴤߩ㉄ൻ቟ቯᕈ

ߩ㉄ൻ቟ቯᕈࠍᬌ⸛ߒߚ⚿ᨐ㧘ࠦࡦ࠻ࡠ࡯࡞ࠃࠅ߽⺞ℂᓟߩ⥄േ㉄ൻ߇㒐ᱛߢ߈ࠆߎߣ߇್᣿ߒߚ‫ߚ߹ޕ‬㧘឴ߍ㧠㧚✚ ᜝⒳ߦኻߒ RTE ࠍ 2%ᷝടߔࠆߣ 1%ᷝടߦᲧߴ᣿⏕ߥ㉄ൻ㒐ᱛലᨐ߇⏕⹺ߐࠇߚ‫ޕ‬એ਄ߩ⚿ᨐ߆ࠄ RTE ߪ᳓ṁᕈߢ޽ࠆߚ߼㧘ᴤ⢽ߦ⋥ធᾲ቟ቯᕈߦఝࠇߚ㉄ൻ㒐ᱛ೷ߣߒߡ㧘ᄤὼࡐ࡝ࡈࠚࡁ
࡯࡞ࠍ฽߻ RTE ߣ Pro ߦ⌕⋡ߒ㧘Toc ߦઍࠊࠆᣂⷙ㉄ൻᷝടߔࠆߩߢߪߥߊ㧘฽᳓♽ߩ឴ߍ⒳ߦᷙߗߡ↪޿ࠆߎ㒐ᱛ೷ߣߒߡߩ೑↪ߦߟ޿ߡᬌ⸛ߒߚ‫ࡈ࡝ࡐߩࠄࠇߎޕ‬ߣߢ㧘ࡈ࡜ࠗᴤߩ㉄ൻࠍലᨐ⊛ߦ㒐ᱛߒ㧘ߐࠄߦࡈ࡜ࠗࠚࡁ࡯࡞฽᦭㊂ߪ㧘RTE㧦35.4%㧘Pro㧦99.3%ߢ޽ࠅ㧘ࡐ㘩ຠߩ቟ోᕈࠍะ਄ߔࠆߎߣ߇ߢ߈ࠆߎߣ߇್᣿ߒߚ‫ޕ‬࡝ࡈࠚࡁ࡯࡞㊂޽ߚࠅߩ DPPH ࡜ࠫࠞ࡞ᶖ෰⢻ߪߘࠇߙ

ᧄᴺߪ᳓ṁᕈߩࡐ࡝ࡈࠚࡁ࡯࡞ൻว‛ߢ޽ࠆ RTE ࠍߘࠇ Toc ߩ⚂ 2 ୚ߣ㧟୚ߢ޽ߞߚ‫ߚ߹ޕ‬㧘࡜ࡦࠪࡑ࠶࠻ⵝ⟎ߦࠃࠆടᾲ㉄ൻ቟ቯᕈ⹜㛎ߩ⚿ᨐ㧘150͠ߩടᾲ᷷ᐲߢ

ߩᕈ⾰ߦวࠊߖߡ೑↪ߔࠆᣂⷙᴺߢ޽ࠅ㧘ᧄᴺߦࠃࠅ
RTE ࠍᴤ⢽ߩടᾲഠൻߦኻߔࠆ᦭ലߥᄤὼ㉄ൻ㒐ᱛ೷ߣߪ RTE ᷝട♽߅ࠃ߮ Pro ᷝട♽ߪ Toc ᷝട♽ߣ߶߷ห╬ߒߡ೑↪ߢ߈ࠆߎߣ߇᣿ࠄ߆ߦߥߞߚ‫ޕ‬♽ߩ⚂ 2 ୚ߩ㉄ൻ቟ቯᕈࠍ␜ߒߚ‫ߚ߹ޕ‬㧘ടᾲᤨߩࡐ࡝ෳ⠨ᢥ₂ᱷሽ₸ߪ RTE ߣ Pro ߢߪ Toc ߿ߘߩઁߩ㉄ൻ㒐ᱛ೷ߦᲧ1)

ߩ㉄ൻ቟ቯᕈࠍ␜ߒߚ߇㧘180͠ߢߪ㧘ਔ♽ߣ߽ Toc ᷝടࡈࠚࡁ࡯࡞ߣ Toc ᱷሽ₸ࠍᲧセߒߚ⚿ᨐ㧘㜞᷷ߦ߅ߌࠆᣣᧄᴤൻቇળ㧔2005㧕ߴߡ㜞ߊ㧘⠴ᾲᕈࠍ␜ߒߚ‫ߢߎߘޕ‬㧘ᴤ⢽ߩടᾲഠൻߦኻߔࠆ㒐ᱛലᨐ߇ᦼᓙߐࠇߚߚ߼㧘ࡈ࡜ࠗടᾲ⹜㛎ࠍⴕᣣᧄᴤൻቇળ✬㧘ᴤ⢽࡮⢽⾰ߩၮ␆ߣᔕ↪㧘p.260,2)

Z. REBLOVA, The effect of temperature on the㧙㧙㩷antioxidant activity of tocopherols, Eur. J. Lipid Res.
Vol. 108, 858-863 (2006)

3) J. POLORNY, Are natural antiozidants better and seferthan synthetic antioxidant?, Eur. J. Lipid Res. Vol. 109,629-642 (2007)4)ኹየ㗅ੑ㧘⧃↰ ဋ㧘ᯏ⢻ᕈࡐ࡝ࡈࠚࡁ࡯࡞㧘ൻቇ5)

I. LAMBROPOULOS and I.G. ROUSSIS, Anti-ߣ↢‛, Vol.44, 688-698 (2006)

oxidative activity of red wine phenolic extracts towards
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K. SOULTI and I.G.ROUSSIS, Inhibition of butter

oxidation by some phenolics, Eur. J. Lipid Res. Vol.109,706-709 (2007)7) S. HARA, R. FUKASAWA and Y. TOTANI; Evaluationof anti-oxidative activities of south-african herbal tea

and grapeseed extracts, J. Oleo Sci. Vol.54, 627-632(2005)

8) R. FUKASAWA, A. KANDA and S. HARA; Anti-oxidative effects of rooibos tea extract of autoxidation

9)and thermal oxidation of lipids, J. Oleo Sci., in press.

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TOTANI;

Modified

potentiometricmethods

withisooctane for peroxide value determination, J. Jpn Oil
Chem. Soc.,Vol 43,18-22 (1994)10) ᣣᧄᴤൻቇળ✬, ၮḰᴤ⢽ಽᨆ⹜㛎ᴺ㧘ᣣᧄᴤൻቇળ㧔2006㧕
11) S. HARA, E. OGAWA and Y. TOTANI; Evaluation ofheat-deteriorated oils –TLC-FID method for determining
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12) Y. KUGE, A. KANDA and S. HARA; Pursuit ofoxidation behavior for conjugated polyenoyl glycerols

and establishment of their novel oxidation prevention
method, J. Oleo Sci., in press㧙㧙㩷