KOSEN

Department of Bioresuorces Engineering
National Institute of Technology, Okinawa College

National Institute of Technology

Development and application of cell-free protein
synthesis systemName

ITO Masaaki

StatusProfessor

E-mailma-itou@okinawa-ct.ac.jp

Affiliations

Japan Society for Bioscience, Biotechnology, and Agrochemistry.
The Japanese Biochemical Society

Keywords

Cell-free protein synthesis system, insect, silkworm, polyphenol oxidase

TechnicalSupport Skills
Research Contents

・ Recombinant DNA experiment・ Screening, purification and analysis of enzymes

Development and application of insect cell-free protein synthesis system

Insect cell-free protein synthesis systemAvailable Facilities and Equipment
Spectrophotometer, DU800（Beckman Couｌter）

Microplate Reader, infinite M200（TECAN）

Spectrophotometer, DU800（Beckman Couｌter）

Microplate Reader, sunrise rainbow thermo（TECAN）

Chromatography system, AKTA avant（GE Healthcare）

Microchip Electrophoresis System, MultiNA（SHIMADZU）

Chromatography system, AKTA prime plus（GE Healthcare）
Cell disruptor, MULTI-BEADS SHOCKER（YASUI KIKAI）

KOSEN
National Institute of Technology

Name

Junsei Taira

Department Bioresources Technology
National Institute of Technology, Okinawa College

E-mailtaira@okinawa-ct.ac.jp

StatusProfessor
・The Pharmaceutical Society of Japan
Affiliations
・American Chemical Society
・Oxidative stress, Antioxidant, Cosmetics, Functional food
Keywords
TechnicalSupport Skills

・Evaluation of functional materials in bioresources
・Isolation of functional compounds

Research Contents
My research target is to find the active compounds as potential useful functional food, pharmaceuticalcosmetics and drugs in bioresources (plants1-4) and marine natural products5-6) and also make the aroma
profiles in beverages 7). The research flow indicated in the under figure and general evaluation is as follows.
Polyphenol content, antioxidant activity (ORAC method, ESR study), anti-inflammatory effect, antitumor
activity, apoptoic activity and inhibition of melanogenesis.
Recent research publications are as follows.
1) Oxidants and Antioxidants in Medical Science. 2, 21-28, 2013
2) Journal of Food Composition and Analysis. 29, 117-125, 2013
3) Journal of Agricultural and Food Chemistry. 62, 167−172, 2014
4) Food Chemistry. 166, 531-536, 20155）Marne Drugs. 10, 2741-2748, 20126) Tetrahedron Letters. 55, 1421-1423, 2014
7) Food Science and Technology Research. 18, 177 -181, 2012

Natural products

AssayAnalysis

Available Facilities and Equipment
Large scale headspace GC/MSLC/MS
LC for analysis and preparative isolation
DNA analyzer

Agilent Technologies
Agilent Technologies
Agilent Technologies
Agilent Technologies

FindDepartment of Bioresources Engineering
National Institute of Technology, Okinawa College

Research of the Okinawan traditional distilled liquor“AWAMORI”
Name

Yasutomo Yamaki

Status

Associate Professor

E-mailtamaki@okinawa-ct.ac.jp

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Affiliations

The society for Biotechnology, JapanThe society of Tropical Resources Technology

Keywords
TechnicalSupport Skills
Research Contents

Awamori, fermentation, microoganizms, Black koji-mold,・ The whole about the AWAMORI. / ・Aromatic component analysis.
・ Alchoholic beverages development of products. / ・Fermented food development.
Research of the microbe which has an influence on the quality of Awamori.

Koji

Moromi

Sampling

Elucidation of
the function

Preservation of
microoganisms

Isolation ofmicrooganisms

Identification ofmicrooganisms

Establishment ofbrewing method

Experimental brewing

Develotment of
new Awamori

Analysis of qualityof liquor

Awamori

Available Facilities and Equipment
名称・型番（メーカー）
Gas Chromatograph Mass Spectrometer （GC-MS : Shimadzu）

Headspace automatic sampler system （Perkin Elmer）

High Performance Liquid Chromatography （HPLC : Shimadzu）

Biophotorecorder （ADVANTEC）

Differential scanning calorimeter (DSC : Shimadzu)

Thermal Analysis (TA : Shimadzu)

Jar fermenter ( Takasugi)

Clean bench (AIRTEC)

Autoclave (Tomy)

Thermo-hygrostat (EYELA)KOSEN

Department of Bioresources Engineering
Okinawa National College of Technology

National Institute of Technology

Bioresources and Photochemistry
Name

HAMADA Taisuke

StatusProfessor

E-mailhamada@okinawa-ct.ac.jp

Affiliations

The Chemical Society of Japan, Japan Society of Coordination
Chemistry, The Electrochemical Society of Japan, Japanese Societyof Engineering Education

Keywords

Photochemistry, Physical chemistry, Coordination chemistry

TechnicalSupport Skills

・ Measurement of photochemical property
・ Supercritical fluid extraction
・ Photo energy conversion

Research Contents[Dye sensitized solar cell]
Solar energy have attracted much attentions because it is clean, and it is called green energy. Solar cellis device to convert photo-energy to electric energy. We have been studied a solar cell, which is called thedye sensitized solar cell (DSSC).
We have made DSSCs and studied the effect of the dye on the efficiency under irradiation of sunlight (AM1.5G) from the solar simulator (Fig. 1).
In this study, we have evaluated the photochemical andphotoelectrical properties of the dye. Therefore, we are able to measure and to evaluate the photochemical
property of various compounds in order to clarify a photochemical ability.
[Super critical fluid extraction]
We have been trying to obtain the useful compound such as poly-methoxy flavonoid (PMF) by means ofsuper critical CO2 extraction method. The merit of this method is that the temperature for the extraction
is relatively low and CO2 dose not remain in an extract.

Available Facilities and Equipment
Solar simulator (WACOM)
Supercritical CO2 extraction apparatus (AKICO)KOSEN

Department of Bioresuorces Engineering
National Institute of Technology, Okinawa College

National Institute of Technology

Search for biodiversity and function of marineorganisms and application to conservation
Name

Naoko Isomura

E-mailiso@okinawa-ct.ac.jp

StatusAssistant Professor
The Zoological Society of Japan, The Ecological Society of Japan,
Affiliations
Japanese Coral Reef Society
Keywords

Corals, Reproduction, Reproductive ecology, Genetic differentiation, Speciation

TechnicalSupport Skills

・ Genotyping using sequences and microsatellite marker
・ Collecting gametes, cross experiments and rearing juveniles of marine organisms
・ Collecting marine organisms by SCUBA・ Microbial community analysis by NGS (not main theme)

Research Contents
In my study, my purposes are to search biodiversity of marine organisms, especially scleractinian corals,
and to apply for conservation.
My purpose is to estimate the genetic diversity and its function of marine organisms in Okinawa. First, Iwill try to clear morphological and/or diversity of species and population. And then, after I search andclarify the endocrine system of organisms by physiological and molecular analyses, I will evaluate therelationship between these system and ecological characteristics of organisms. Moreover, I will investigate
conservation method for target organisms using my results.

Available Facilities and Equipment
Capillary Sequencer: CEQ8800 (Beckman Coulter)

Next Generation Sequencer MiSeq (illumine)KOSEN

Department of Bioresources Engineering
National Institute of Technology, Okinawa College

National Institute of Technology

Cloning of the gene for plant stressresponsiveness
NameSANMIYA Kazutsuka

Status

Associate professor

E-mailsanmiya@okinawa-ct.ac.jp

Affiliations

The Japanese Society of Plant Physiologists
The Molecular Biology Society of JapanKeywords

plant stress responsive gene, isoprenoid, heat shock protein, saponinTechnical
Support Skills

・ Gene cloning
・ Gene expression analysis
・ Transgenic plantResearch Contents

FPPS gene, heat shock protein gene, UGT gene, Rice yield● Cloning of the gene for FPPS
For the first time, the gene for chloroplast FPPS was cloned.
FPPS is the key enzyme for isoprenoid biosynthesis.
Isoprenoids play roles in plant stress response such as protection against sunlight.
● Heat tolerant transgenic tobacco
The gene for heat shock protein was used for the transformation (Fig).
● Cloning of the gene for UGTFor the first time, the gene for saponin biosynthesis was cloned.
Saponin protects the Brassicaceae crops, cabbage, radish, broccoli against pests.
● Rice yield increased to 124% by glass silica

wild typeheat toleranttobaccotransgenic tobacco

Available Facilities and Equipment
Electrophoresis apparatus Mupid (ADVANCE)
Gene expression analyser AE6933 (ATTO)
Thermal cycler TP450 (TaKaRa)KOSEN

Department of Bioresources Engineering
National Institute of Technology, Okinawa College

National Institute of Technology

Application of shock wave processing technology
to biological materials
NameTakemoto, Ayumi

Status

Associate Professor

E-mailtkmt@okinawa-ct.acjp

Affiliations

The Japan Society of High Pressure Science and Technology
The Japan Society of Cookery Science
The Materials Research Society of Japan

Keywords

Shockwave processing, Non-thermal sterilization, Hands-on Display

Technical
Support Skills

The Gemmological Society of JapanThe Museological Society of Japan・ Shock wave processing to biosystem
・ Non-thermal milling, Efficient extraction

Research Contents
The instantaneous high pressure, that is, the shock wave divides into the penetration wave and thereflected wave on the surface of the density difference. The shock wave divides into the penetration wave
and the reflected wave at the surface of the density difference. The reflected wave caused on a high densityside brings the pull stress from negative pressure. Because this high-speed destruction phenomenon , it isexpected the non-thermal sterilization, food processing, extraction, and so on.
Shock wave molding technology called "the Explography" is a metal molding technology that uses highpressure generated by a supersonic speed shock wave for plastic processing. By using the Explography, it ispossible to three-dimensionally form onto a material plate such as a plant which is difficult to use in staticpress processing. This molded article can be applied to a hands-on exhibition in a museum, a teachingmaterial in a blind school.

Rice flour milled by instantaneous high-pressure

Available Facilities and Equipment
Rheometer , RE2-33005C (YAMADEN co.,ltd)
Scanning Electron Microscope, JCM-6000 (JEOL Ltd.)The metal plant specimen by shock wave moldingKOSEN

Department of Bioresources Engineering,
National Institute of Technology, Okinawa College

National Institute of Technology

Application of Okinawan microorganisms
NameToshiaki TANABE

Status

Associate Professor

Affiliations
Keywords
TechnicalSupport Skills

E-mailtanabe@okinawa-ct.ac.jp

JSBBA, SBJ, JSMSB, JSCC, MSJ, and BSO
Chitin, Chitosan, White-rot fungi,
・ Component analysis of biomass
・ Screening of bacteria or enzymes for bioremediation or bioconversion

Research ContentsConstruction of Okinawan Soil library
The soil samples were collected from approximately 2,600 places including each remote island in Okinawa,and were constructed to the Okinawan soil library. Various kinds of microbes are isolating from this library.
They have various bioactive substances and enzymes.
Construction of the soil microbe library
The soil microbe library was constructed with each microbe group isolated from each soil sample. Thislibrary includes about 500 strains of bacteria utilizing alkane, 500 strains of siderophore-like materialproducing bacteria, 1,700 strains of Actinomyces, 500 strains of Basidiomycetes.
Application of biologically active substances
Bioactive substance library has been constructed from the extracts of the soil microbe. For example,
siderophore is an iron chelating agent which microbe produce. It is thought that siderophore is able to applyfor anticancer.
Application to bioremediation
The isolated bacteria utilizing alkane are able to use for cleaning of the soil polluted with oil. In addition,
the white-rot fungi degrading high selectively lignin are able to remediate the soil polluted by dioxin or PCB.

Available Facilities and Equipment
Bioshaker G B-200(Taitec)
Electrophoretic apparatus（GE Healthcare Japan）

;.ÛÅÑæÞâçëåßâÐw rÐÚÉØw»Õo»Biodiversity of Oribatid mites in Ryukyu
Islands
NameWataru HAGINO

E-mailhagino@okinawa-ct.ac.jp

StatusAssistant Professor
The Acarological Society of Japan, The Japanese Society of Soil
Affiliations
Zoology, Japanese Society of Systematic Zoology

Keywords

Soil fauna, Oribatid mites, Biological indicator, Ecology, Taxonomy

Technical
Support Skills

ð Taxonomical study of Oribatid mites (Order Acari; suborder Oribatida).
ð Development of the biological indicator used by soil organisms.
ð Experience-based program on biodiversity of our familiar environment.

Research Contents
Oribatid mites (Fig. 1) are small arthropods that are also called beetle mites, armored mites, or moss mites.
They comprise more than 10,000 species. Most oribatid mites inhabit the soil–litter system; often, they are
the dominant arthropods in temperate-forest soil.
å The ultimate goal of my research is to clarify the entire Japanese oribatid fauna. Toward this goal, I
performed a series of taxonomic studies targeting the Oribatid fauna of subtropical regions in Japan(=Ryukyu Islands), by using morphological and molecular biological methods.

(Fig. 1) Various Oribatid mites in Japan.

Available Facilities and Equipment