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Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

IntegriSense™ 680
Product Number: NEV10645

DESCRIPTION: IntegriSense™ 680 is a targeted fluorescence imaging agent comprising a potent, selective non-peptide

small molecule integrin αvβ3 antagonist and an NIR fluorochrome. This agent has been developed to enable in vivo
visualization and quantification of integrin αvβ3 expression in neovasculature as well as in tumor cells, to monitor tumor
angiogenesis, growth and treatment efficacy. Half-life in tissue of IntegriSense 680 signal is >21 days.

MATERIAL (Needs to be reconstituted)

CONTENTS: Each vial contains 24 nmol of IntegriSense
680 lyophilized solid. The IntegriSense 680 solution has been
filtered through a 0.2 µm filter prior to lyophilization.
Reconstitute IntegriSense 680 with 1.2 mL of 1 x PBS before
injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice (weighing~25 grams each) when using the recommended dose of 2 nmol(100 µL) of IntegriSense 680 per mouse.

Table 1. IntegriSense 680 Characteristics
Property
Specification
MW1432 g mol-1
Fluorescence1
 Excitation
675 nm Emission
693 nmAbsorbance
675 ± 5 nm
Purity2>95 %Appearance
Dark blue solid

1. Absorbance and fluorescence maxima of IntegriSense 680 in 1x PBS.
2. As determined by RP-HPLC and measuring absorbance at 675 nm.

PROPERTIES: The physical characteristics of1IntegriSense 680 can be found inTable 1 and Figure 1.

Page 1 of 2A.U.

STORAGE & HANDLING:

0.8
0.6
0.4

Upon receipt, IntegriSense 680 should be
0.2
IMMEDIATELY STORED AT 2-8 oC AND
0PROTECTED FROM LIGHT.
400500600700800
When stored and handled properly, IntegriSense
680 is stable for up to twelve months in theWavelength (nm)lyophilized form.
Before opening the vial check to ensure that thelyophilized powder is present at the bottom of theFigure 1. Absorbance and fluorescence emission spectra ofIntegriSense 680 in 1x PBS.
vial.
After reconstituting with PBS, gently swirl thesolution to ensure that the lyophilized powder isfully in solution.
Once reconstituted with 1 x PBS, the solution is stable up to 10 days when stored at 2-8 ºC and protectedfrom light.

900

IN VIVO IMAGING AND APPLICATIONS:The generally recommended procedure for in vivo imaging with IntegriSense 680 is administration via intravenous
injection and imaging 3 – 48 hours post injection.
Imaging at earlier time points (~3 hrs) is recommended when imaging the vasculature
Imaging at later time points (24-48 hrs) is recommended when imaging tumors to reduce background.
IntegriSense 680 enables imaging of neovasculature and tumors in a range of oncology applications.

NOTES:
PerkinElmer’s IntegriSense 680 is intended for research purposes only and is not for human use. It must be used byor directly under the supervision of a technically qualified individual experienced in handling potentially hazardous
materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

PerkinElmer, Inc.
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P: (800) 762-4000 or (+1) 203-925-4602
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