TECHNICALDATASHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

MMPSense™ 645 FAST
Product Number: NEV10100

DESCRIPTION: MMPSense™ 645 FAST a member of a family of activatable fluorescent imaging agents comprising anovel architecture termed F.A.S.T. (Fluorescent Activatable Sensor Technology), that confers an improved pharmacokinetic
profile with earlier imaging time points. This architecture offers higher target specific signal with reduced background while
also reducing the optimal imaging time after injection.
MMPSense 645 FAST is a matrix metalloproteinase (MMP) activatable agent that is optically silent upon injection andproduces fluorescent signal after cleavage by disease related MMP’s. Activation can occur by a broad range of MMP’s
including MMP 2, 3, 7, 9, 12, and 13. MMP activity is involved in many disease-related processes including cancer
progression, invasion and metastasis, rheumatoid arthritis, pulmonary diseases and areas of cardiovascular disease. MMPSense
645 FAST may be used to monitor the progression of these diseases or to evaluate the potential therapeutic efficacy of drugs
targeting the underlying mechanisms involved in these diseases.

MATERIAL: (Needs to be reconstituted)
CONTENTS: Each vial contains 48 nmol of MMPSense
645 FAST in dry solid form. MMPSense 645 FAST has been
filtered through a 0.2 µm filter prior to drying.
Reconstitute MMPSense 645 FAST with 1.2 mL of 1 x PBS
before injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 4 nmol (100 µL) of MMPSense 645 FAST per mouse.

PROPERTIES: The physical characteristics ofTable 1. MMPSense 645 FAST Characteristics
PropertySpecification
MW~ 43,000 g mol-1Fluorescence1
 Excitation
649 nm Emission
666 nm1Absorbance
649 nm (activated)
Purity2>90%
AppearanceBlue solid
1. Absorbance and fluorescence maxima of MMPSense 645
FAST in 1x PBS.
2. As determined by RP-HPLC and measuring absorbance at645 nm.

MMPSense 645 FAST can be found in Table 1 and Figure 1.
1STORAGE & HANDLING:
Upon receipt, MMPSense 645 FAST should beIMMEDIATELY STORED AT
2-8 oC AND PROTECTED FROM LIGHT.
When stored and handled properly, MMPSense 645
FAST is stable for up to 6 months in dry solid form
from shipment date.
Before opening the vial check to ensure that all of thesolid material is at the bottom of the vial.
After reconstituting with PBS, gently swirl thesolution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution isstable up to 10 days when stored at 2-8 ºC andprotected from light.

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Figure 1. Normalized absorbance (grey), excitation(blue) and fluorescence emission (red) spectra ofMMPSense 645 FAST in 1x PBS.

IN VIVO IMAGING AND APPLICATIONS:The generally recommended procedure for in vivo imaging with MMPSense 645 FAST is administration via
intravenous injection and imaging 6-72 hours post injection.
MMPSense 645 FAST will clear from tissues after approximately 96 hours. Repeat injection and imaging may be
performed every four days for longitudinal studies. It is recommended that a pre-injection baseline image be takenprior to reinjection and imaging.
MMPSense 645 FAST enables imaging of tumors and inflammation in a broad range of oncology, pulmonary andcardiovascular applications.

NOTES:
PerkinElmer’s MMPSense 645 FAST is intended for research purposes only and is not for human use. It must beused by or directly under the supervision of a technically qualified individual experienced in handling potentiallyhazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S. and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement withPerkinElmer.

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