TECHNICALDATASHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

OsteoSense® 800
Product Number: NEV11105

DESCRIPTION: OsteoSense® 800 is a fluorescent in vivo diphosphonate imaging agent. OsteoSense 800 images areas of
microcalcification and bone remodeling and enables imaging of bone growth and resorption.

MATERIAL (Needs to be reconstituted)

CONTENTS: Each vial contains 24 nmol of OsteoSense
800 in dried solid form. OsteoSense 800 solution has been
filtered through a 0.2 µm filter.
Reconstitute OsteoSense 800 with 1.2 mls of 1x PBS before
injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 2 nmols (100 µL injection volume) of OsteoSense 800 permouse.

Table 1. OsteoSense 800 Properties
Property
Specification
MW~ 1281 g mol-1
1Fluorescence Excitation
780 nm Emission
805 nmAbsorbance
790 nmPurity2>95 %Appearance
Dark blue-green
Solid1.
2.

Absorbance and fluorescence maxima of OsteoSense 800 in PBS.
As determined by SE-HPLC and measuring absorbance at 750 nm.

Fig 1.

PROPERTIES: The physical properties of OsteoSense
800 can be found in Table 1 and Figure1.

STORAGE & HANDLING:


Upon receipt, OsteoSense 800 should be STORED
AT 2-8 oC AND PROTECTED FROM LIGHT.
When stored and handled properly, OsteoSense 800 is
stable for up to 12 months in dry solid form.
Before opening the vial check to ensure that all of thesolid material is at the bottom of the vial.
After reconstituting with PBS, gently swirl thesolution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution isstable up to 10 days when stored at 2-8 ºC andprotected from light.

Absorbance and fluorescence emission spectra of OsteoSense 800 in 1x PBS.

IN VIVO IMAGING AND APPLICATIONS:The recommended procedure for in vivo imaging with OsteoSense 800 is administration via intravenous injection andimaging 24 hours post injection.
OsteoSense 800 signal half-life in bone is 7-12 days

Page 1 of 2Imaging Bone Growth: OsteoSense 800 can be used to measure the effects of therapeutic stimulation of bonegrowth.
Imaging Bone Remodeling: OsteoSense 800 can be used to characterize bone remodeling associated with animalmodels of arthritis.

SELECTED REFERENCES:
Zaheer, A., Lenkinski, R.E., Mahmood, A., Jones, A.G., Cantley, L.C., Frangioni, J.V.,
In vivo near-infrared fluorescence imaging of osteoblastic activity. Nature Biotechnology 19, 1148-1154 (2001).

NOTES:
PerkinElmer’s OsteoSense 800 is intended for research purposes only and is not for human use. It must be used byor directly under the supervision of a technically qualified individual experienced in handling potentially hazardous
materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

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