More Value from Milk
With Pall comprehensive fractionation assortment
FBMFMILKEN

More value from milk with
Pall comprehensive fractionation assortment
Pall® crossflow membranes and chromatography sorbents equip
process separation systems with new options for purifying milk
proteins and peptides for higher yields and faster profits.
Dairy manufacturers will benefit from extracting
higher profit proteins and peptides from milk.
Fractionation techniques have evolved
dramatically in recent years. Techniques
previously restricted to specialized laboratories,
can now be performed at an industrial level.
Chromatography technology is the ideal
complement for the separation of pure milk
into whey proteins, peptides, enzymes andgrowth factors.
Pall, the world’s leading filtration, separation,and purification company, has acquired
Biosepra. The Biosepra® product line provides
qualified chromatography sorbents to foodingredients production. With this acquisition,
Pall’s product portfolio comprises a uniqueoffering of preparative chromatography
solutions.

Pall comprehensive
fractionation assortment
Consistency
With total revenue of $2 billion and more than
30 years serving the Food and Beverageindustry, our reputation depends on
consistency of performance - in products, indocumentation, and in service and customer
support systems. This principle is embedded
in our culture.

Innovation
In the development of dairy fractionation
systems, it is necessary to think large and act
small. Being a large company allows substantial
knowledge and resources to be applied for
product development. It also enables timely
investment in the necessary validation andmanufacturing programs to bring new products
to market consistently and reliably. Pall knows
the value of small teams, working withcustomers and other innovators to bring new
ideas to fruition. The large product portfolio andthe number of highly skilled teams result innumerous solutions - recognizing that each milk
fractionation process is unique and makes
specific demands.

ServiceWith facilities and dedicated personnel in every
global region, whether it is at the start of a
project or after installation, a quick response isdelivered in every case. Pall specialists areexperienced and trained to reply to anyquestions on process development andoperation, including general purification andfiltration, instrumentation and documentation,
as well as chromatography sorbents andcrossflow membranes. Additionally, unique andvaluable technology services exclusive to Pall,such as purification development using protein
chips arrays, and column packing diagnostics,
help deliver further advantages in speed andeffectiveness of service.

Membralox® ceramic membranes and modules

Membralox ceramic membranes areasymmetric membranes composed of
porous support and filtering layers.
Membrane materials (alumina, zirconia,titania) provide a high resistance to a wide
range of pH, chemicals, temperature andpressure. High compactness of themulti-channel membrane combined withhigh permeability allow treatment of large
volumes with high throughput.

Applications

The Membralox GP membranes have alongitudinal permeability gradient whichallows for homogeneous and calibrated
permeation along the flow channel
resulting in marked improvement ofutilization of membrane surface withina stable microfiltration regime.

• Proven long operational life

• Microbial removal from milk and whey
• Defatting of milk and whey
• Casein separation from whey proteins• Protein standardization

Features• High flux
• High controlled selectivity• Wide chemical and pH (0-14) compatibility• Excellent thermal stability• Sanitizable and sterilizable• 100% bubble point integrity testing• Hydrodynamically optimized
GP membranes• Customized membrane configurations
can also be proposed for the mostdemanding applications

What does it mean for you?
More productivity• Significant increase in protein yields• Selective separations between casein andsoluble proteins• Selective fractionation of high value
milk components• Longer service life, longer processing times• Shorter and simpler cleaning cycles• Typical 6 Log reduction of bacteria

Membralox membranes

Membralox modules

Membralox modules

Pall Biosepra® chromatography sorbents

The dairy dedicated line of Pall Biosepra
products comprises of different sorbents
which are used to capture and purify
proteins such as alphalactalbumin,
betalactoglobulin, lactoferrin, andlactoperoxydase from milk and whey.
The Pall ceramic HyperD® range ofsorbents are rigid porous beads, which
are coated and permeated with afunctionalized cation and anion exchangehydrogels. This gives the beads
outstanding rigidity and flow performanceas well as exceptional mass transfer anddynamic properties.
The SPEC 70 SLS sorbent is aspherical semi-rigid microbead ofacrylic copolymers that generate low
back-pressures at typical low pressureliquid chromatography process flow rates.
The strongly-bound cation exchangegroups, which are an integral part of thepolymer structure, are found within its
three dimensional structure. Each
sub-unit of the polymer possesses
hydrophilic groups which ensure
biocompatibility and prevent
non-specific adsorption.

Applications• Dairy protein capture and purification• Dairy polypeptide and peptide purification

Features• High dynamic binding capacity athigh flow rates• Easy, simple cleaning withsodium hydroxide• Allow large volume processing• Not sensitive to fouling/clogging inviscous and/or lipidic solutions• Highly resistant to microorganisms

What does it mean for you?
Cost effectiveness andhigh resolution• High productivity, large throughput(up to 50000L/h of milk or whey)• High molecule purity (> 85%)
• Easy and cost effective regeneration• Long service life (typical 2 to 7 years)• Short and simple cleaning cycles

Sorbent

Microza* PES hollow fiber ultrafiltrationmodules for dairy applications
Microza hollow fiber ultrafiltration
modules comprise a unique
double-skinned proprietary polysulfone
membrane with dense internal layer.
Currently available in 4000, 6000 and
10000 molecular weight cut-off, themodules are used in a range ofapplications including pyrogen removalfrom water, chromatography buffer
solutions and protein concentration.

Application
Concentration of protein downstream
chromatography elution

Features• Double skin provides assurance ofremoval rating• Combination of high strength and high flow
due to unique membrane construction• High strength fibres allow reverse filtration
to reduce fouling• Can be hot water sanitized up
to 95°C (203°F)• Compatible with a wide range of cleaning
agents, up to 5000 ppm sodium
hypochlorite or 4% sodium hydroxide

What does it mean for you?
Ease of use and productivity• No recontamination of theconcentrated protein• High protein concentration andpurification yields• Long running time, low sensitivity to fouling• Short and easy cleaning cycles

Microza module

Microza hollow fibertransversal cut

Microza hollow fiberMore value from milk withPall comprehensive fractionation assortment
Pall Biosepra Chromatography Services
Supporting its global network of processing specialists, Pall also has additionalcustomized services.

On Protein Chip Purification
Development and Identification
Available in Pall laboratories around the world,Protein Chip technology, used with arrays ofsorbent chemistry provides a very fast method
to assess samples for binding and elution ofmolecules under different conditions. This
process development tool saves valuable timeand generates more valuable information thatconventional operation of small scouting
columns alone. Samples may be assessed in
our own laboratories and we can also work withcustomers to develop in-house capability. Inaddition to its use in purification development,
this service can be used for expression
monitoring and product analysis of protein.

Packing Studies
The consistency in performance of achromatography column is highly dependent
upon the effectiveness of the packing method.
Pall offers the ability to run trials, to optimize
packing methods and confirm bed stability on
repeated cycles.

Process Optimization
By using some of these tools in tandem withall conventional methods we are ready to offer
specific guidance where our own customer’s
resources may be stretched or where
additional data is required quickly. In the mostdemanding critical situations we can often
make the difference.

Training
Based on a broad process expertise, Pall has
developed training modules for many aspects ofpurification and separation technology including
filtration and chromatography. They are bothadvanced scientific and at basic operator
training levels. These services can be tailoredto specific customer needs.

Food Use Compliance
Please contact Pall for further information on
regulatory food use compliance.

What does it mean for you?
A perfect match of your process needs with a comprehensive
support and an optimized products assortment

Pall Food and Beverage

Visit us on the web at www.pall.com/foodandbev

Portsmouth - UK
+44 (0)23 9230 2269 telephone+44 (0)23 9230 2509 fax
processuk@pall.com

Pall Corporation has offices and plants throughout the world in locations including:
Argentina, Australia, Austria, Belgium, Brazil, Canada, China, France, Germany, India,Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico, the Netherlands, New Zealand,Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden, Switzerland,Taiwan, Thailand, United Kingdom, United States, and Venezuela. Distributors are locatedin all major industrial areas of the world.

New York - USA
866 905 7255
toll free+1 516 484 3600
telephone+1 516 625 3610
fax
foodandbeverage@pall.com

Because of developments in technology these data or procedures may be subject to change. Consequently
we advise users to review their continuing validity annually. Part numbers quoted above are protected by theCopyright of Pall Europe Limited.
, Pall, Biosepra, HyperD and Membralox are trademarks of Pall Corporation.
*Microza is a trademark of the Asahi Chemical Company.
Filtration. Separation. Solution. is a service mark of Pall Corporation.
® indicates a trade mark registered in the USA.
©2006, Pall Corporation.

January 2007. Printed in England.

FBMFMILK/0107