TECHNICAL
DATA
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Fluorescent Imaging Agent
Caution: For laboratory use only. This product is intended for animal research only and not for use in humans.

Name: VivoTrack 680 Sample Size
Product Number: NEV12001
DESCRIPTION
VivoTrack 680 is a near infrared fluorescent cell labeling agent that intercalates into the plasma membrane of primary cells and cell lines.
Cells are brightly labeled and retain excellent viability and function, with the near infrared wavelength of the fluorescence offering optimal invitro and in vivo detection.

CONTENTS Each vial contains 0.2 mg of labeling agent formulated inpolyethylene glycol and is in dry solid form.
 The packaged material in each vial provides sufficient reagent forup to 2 x 108 cells.

STORAGE & HANDLING Upon receipt, VivoTrack 680 should be IMMEDIATELY STORED
AT 4 °C AND PROTECTED FROM LIGHT.
 When stored and handled properly, VivoTrack 680 in its dry solidform is stable for up to three months.
 Before opening the vial check to ensure that all of the solid material
is at the bottom of the vial.
 After reconstituting with 1X PBS, gently swirl the solution to ensurethat the solid is fully in solution.
 Once reconstituted, VivoTrack 680 is stable for up to 14 days
when stored at 2-8 °C and protected from light.
 Allow reconstituted VivoTrack 680 imaging agent to equilibrate toroom temperature before introducing into animals.

APPLICATIONSPHYSICAL AND SPECTRAL PROPERTIES
Property
MWFluorescence emission1
Absorbance1
Purity2Appearance

Specification~1173 g mol-1
696 nm
676 nm>90 %Light blue solid

1. Absorbance and fluorescence maxima in ethanol.
2. As determined by RP-HPLC; measuring absorbance at 675 nm

Normalized absorbance (blue) and fluorescence emission (red) spectra of
VivoTrack 680 in ethanol Whole cell in vitro labeling of primary cells and cell lines for NIR
fluorescence microscopy.
 Whole cell in vitro labeling of primary cells and cell lines for in vivo tracking by either ex vivo flow cytometry or in vivo noninvasive NIR
fluorescence imaging.
 Whole cell in vitro labeling of tumor cells and cell lines for orthotopic implantation and in vivo noninvasive NIR fluorescence monitoring.

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NOTES Caution: For laboratory use only. This product is intended for animal research only and not for use in humans. It must be used by ordirectly under the supervision of a technically qualified individual experienced in handling potentially hazardous materials. Please read theMaterial Safety Data Sheet (MSDS) provided for this product.
 Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents pending. Our products
are not available for resale or other commercial uses without a specific agreement from PerkinElmer.

General Protocol for Labeling Cells with VivoTrack 680
Materials
 VivoTrack 680 cell labeling agent
 1x PBS
Procedure
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Wash the cells of interest once with sterile PBS or serum-free medium to remove serum proteins and lipids that may interfere withcell labeling.
Discard the supernatant and resuspend the cells (up to 250 x 106 cells/mL) in 2.0 mL of PBS in a 50 mL sterile conical tube.
Dissolve VivoTrack 680 (0.2mg of dye in 1g of PEG) in 1.3 mL of warm sterile PBS (37 °C) and mix by vortex until completelydissolved. This will yield 2.0 mL of the labeling agent.
Add 2.0 mL of the cell labeling solution to 2.0 mL of cells, and mix immediately by gentle vortexing. [Note: It may be required tooptimize dose depending on the cells to be labeled.]
Incubate the cells for 15 min at room temperature, protected from light.
Dilute the cells for washing by adding 15-20 mL sterile RT PBS containing 1% FBS or complete medium, depending on yourultimate use for the cells.
Wash the cells 3 times with RT PBS containing 1% FBS to remove excess cell labeling agent. A final resuspension with sterile
PBS alone can be used to decrease the FBS levels in the cell preparation.
Count, culture, or transfer cells as required by the application.
VivoTrack 680 is highly soluble in aqueous solution and, thus, does not need to transition from organic to aqueous solution upon
addition to the cell suspension. This allows quick dispersion into the cell medium, and very uniform cell labeling is generallyachieved.

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