TECHNICALDATASHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

OsteoSense® 750 EX
Product Number: NEV10053EX

DESCRIPTION: OsteoSense® 750 EX is a fluorescent in vivo bisphosphonate imaging agent. OsteoSense 750 EX
images areas of microcalcifications and bone remodeling and enables imaging of bone growth and resorption.

MATERIAL: (Needs to be diluted)

CONTENTS: Each vial contains 48 nmol of OsteoSense 750 EX as a lyophilized solid. The solution has been filtered(0.2 µ) prior to lyophilization. Upon dilution with 1.2 mL of 1 x PBS, this material provides sufficient reagent for imaging
approximately 10 mice (weighing ~25 grams each) when using the recommended dose of 4 nmol/100 µL 1xPBS ofOsteoSense 750 EX per mouse.

PROPERTIES: The physical properties of OsteoSense
750 EX can be found in Table 1 and Figure 1.

STORAGE & HANDLING:


Upon receipt, OsteoSense 750 EX should be
IMMEDIATELY STORED AT 2-8 oC ANDPROTECTED FROM LIGHT.
When stored and handled properly, OsteoSense 750
EX is stable for six months from date of shipment.
Once reconstituted, the PBS solution is stable up to10 days when stored at 2-8°C and protected fromlight.

Table 1. OsteoSense 750 EX Properties
Property
Specification
MW (free acid)1101.1 g mol-1Fluorescence1
 Excitation
749 ±10 nm
 Emission max
770 ±10 nm
Absorbance max2749 ±5 nm
Purity3>95%
AppearanceBlue solid
1. Absorbance and fluorescence maxima of OsteoSense 750 EX in PBS.
2. Based on concentration resulting in absorbance of 0.3 to 0.5 AU.
3. As determined by RP-HPLC and measuring absorbance at 750 nm.

Fig 1.
1The recommended procedure for in vivo imaging
with OsteoSense 750 EX is administration via tailvein injection and imaging 24 hours post tail vein
injection.
Imaging Bone Growth: OsteoSense 750 EX can beused to measure the effects of therapeutic stimulationof bone growth.
Imaging Bone Remodeling: OsteoSense 750 EX canbe used to characterize bone remodeling associatedwith animal models of arthritis.

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0.8
0.6

Normalized units

IN VIVO IMAGING & APPLICATIONS:

0.40.2

0450

550

650
750
Wavelength, nm

850

Absorbance and fluorescence emission spectra in 1x PBS.

SELECTED REFERENCES: Zaheer, A., Lenkinski, R.E., Mahmood, A., Jones, A.G., Cantley, L.C., Frangioni, J.V.
In vivo near-infrared fluorescence imaging of osteoblastic activity. Nature Biotechnology 19, 1148-1154 (2001)

NOTES:
PerkinElmer’s OsteoSense 750 EX is intended for research purposes only and is not for human use. It must be usedby or directly under the supervision of a technically qualified individual experienced in handling potentiallyhazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S. and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement withPerkinElmer.

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