Data Sheet FBDSGDLEN

GeneDisc® Technologies
For an easy, rapid and specificdetection of Listeria in food andenvironmental samples
Listeria monocytogenes is an opportunistic foodborne pathogenthat can affect a wide range of food products and can cause
severe infections with high mortality rate to the elderly,
immunodepressed persons and pregnant women. Since L.
monocytogenes has the ability to grow slowly at refrigerated
temperatures, it is a major concern for food companies.
Two factors have also raised interest for Listeria spp. detection.
Cases of listeriosis related to other Listeria spp. have beenreported. Furthermore, evidence suggests the presence ofother non-pathogenic Listeria spp. can be an early indicationof L. monocytogenes contamination.
Pall GeneDisc Technologies provides solutions for foodprocessors in need of a reliable control of L. monocytogenes
and/or Listeria spp. risk.

GeneDisc System Benefits
Rapid — Accelerate your controls workflow and achieve fastreleases of short shelf life products and testing of raw materials.
While other methods such as immunoassays or culture
methods (chromogenic media) require up to 3 days to get aresult, Pall’s GeneDisc method allows a detection of Listeria in
as fast as 20 hours.
Easy to use — GeneDisc solutions are designed for routine
use. Implementing PCR (Polymerase Chain Reaction) has
never been this easy.
Modular — System modularity fits your throughput needs: up
to 96 samples can be analyzed in a one hour PCR run.

A Solution Designed For Food Industries
In line with MLG 8, BAM 10, OMA 993.12 and ISO 11290 –NF VALIDATION and AOAC certified method.
Adaptive to testing needs – Simultaneous or individual
testing solutions for L. monocytogenes and Listeria spp. areavailable with same hands-on time and enrichment.

Listeria monocytogenes Identification
Bacteria

Gram + bacilli

Food Vehicle

Large variety of food especially dairy, raw meatand ready-to-eat productsDiseaseListeriosis – can cause severe, invasive infections(e.g., sepsis, meningitis, fetal death)

Incidence of Cases(per 100,000 population)

0.28 (FoodNet, 2010)

Notification Rate(per 100,000 population)

0.35 (EFSA, ECDC, 2010)

Related Outbreaks

3 (strong evidence) (EFSA, ECDC, 2010)
7 (CDC, 2010)

Related Recalls

102 (FDA & FSIS, 2012)

Related Alerts / Information

106 (RASFF, 2011)

How the System Works

Validations
3ionactxtr
AeDNScanbaGe rcod
ne e o
Dis f tc p helate

2

Validation

MatrixTime to Result

AOAC

Variety of foods and selectedenvironmental surfaces

26 h

NF VALIDATION

Food products and environmental
samples

26 h

Raw milk

20 hOrdering Information
1

Enrichment

Load DNA samples

4

Part Number

Description

Samples/packEquipment

Resultsislysna
rt aSta6

Technical Information
Down to 18 hours

Sample Preparation Time< 1 hour for 48 samples

PCR Cycle Time< 1 hour

GeneDisc Ultra-Lyser-

EGDCV3A

GeneDisc Cycler Base Unit

-EGDSV3A

GeneDisc Cycler Sub Unit-

PFOOD1100

Extraction Pack Food 1100

GLISMON206006
GLISMON212006

GeneDisc Listeria monocytogenes

3672

GLISSPP106006
GLISSPP112006

GeneDisc Listeria spp.

3672

GLISDUO106006
GLISDUO112006

GeneDisc Listeria DUO(Listeria monocytogenes and Listeria spp.)

36
72

Consumables
5

Enrichment Time

EGDUL1A230 (EU)EGDUL1A120 (US)

Total Turnaround Time

Down to 20 hours

Hands On Time

About 45 minutes for 48 samples(<1 min/sample)

Limit Of Detection

1 bacteria in 25 g of food sample and inenvironmental samples

Specificity

Wide range of strains tested for inclusivity andexclusivity

Internal Positive Control Per
Sample Analysis

Detects presence of inhibitors in DNA extract
sampleWe also offer a full product range for pathogen detection in foodand water and for spoilage organisms in beverage.
Quantitative tests for pathogens in water (Legionella, E. coli,Enterococcus…) are also available.
For more information including part numbers please contact us.

GEN 25/08 – 07/10, GEN 25/07 – 07/10
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR certificationwww.afnor-validation.org

Visit us on the Web at www.pall.com/genedisc

Pall GeneDisc Technologies
25 Harbor Park Drive
Port Washington, NY 11050+1 516 484 3600
telephone+1 866 905 7255
toll free US

International Offices
Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria,Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico,
the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden,Switzerland, Taiwan, Thailand, the United Kingdom, the United States, and Venezuela. Distributors in all majorindustrial areas of the world. To locate the Pall office or distributor nearest you, visit www.pall.com/contact.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.

genedisc@pall.com© 2014, Pall Corporation. Pall,, and GeneDisc are trademarks of Pall Corporation. ® indicates a trademark
registered in the USA and TM indicates a common law trademark. Filtration.Separation.Solution.SM is a service
mark of Pall Corporation.

FBDSGDLEN

January 2014