TECHNICALDATASHEET

Fluorescent Imaging Agent
Caution: For laboratory use only. This product is intended for animal research only and not for use in humans.

BombesinRSense™ 680
Product Number: NEV10090
DESCRIPTION
BombesinRSense™680 is a fluorescently-labeled peptide designed to bind to bombesin receptors involved in cancer cell stimulation ofproliferation.

 Each vial contains 24 nmol of BombesinRSense 680 in dry solidform. BombesinRSense 680 has been filtered through a 0.2µm
filter prior to drying.
 Reconstitute BombesinRSense 680 with 1.2 mL of 1X PBS before
administering to animals.
 The packaged material provides sufficient reagent for imaging
approximately 10 mice (weighing ~25 grams each) when using therecommended dose of 2 nmol (in 100 μL of PBS) ofBombesinRSense 680 per mouse.

STORAGE & HANDLING Upon receipt, BombesinRSense 680 should be IMMEDIATELYSTORED AT 2-8 °C AND PROTECTED FROM LIGHT.
 When stored and handled properly, BombesinRSense 680 in its
dry solid form is stable for up to three months.
 Before opening the vial check to ensure that all of the solid material
is at the bottom of the vial.
 After reconstituting with 1X PBS, gently swirl the solution to ensurethat the solid is fully in solution.
 Once reconstituted, BombesinRSense 680 is stable for up to 7
days when stored at 2-8 °C and protected from light.
 Allow BombesinRSense 680 imaging agent to equilibrate to room
temperature before introducing into animals.

IN VIVO IMAGING AND APPLICATIONSPHYSICAL AND SPECTRAL PROPERTIES
Property
MW
Fluorescence emission1
Absorbance1
Purity2Appearance

Specification~24,000 g mol-1
694 nm674 nm>95 %Blue solid

1. Absorbance and fluorescence maxima in 1x PBS.
2. As determined by RP-HPLC; measuring absorbance at 670 nm.
1.20

Normalized Intensity

CONTENTS

1.00
0.80
0.60
0.40
0.20
0.00
400

500

600

700

800

900

Wavelength nm

Normalized absorbance (blue) and fluorescence emission (red) spectra ofBombesinRSense 680 in 1x PBS

 The generally recommended procedure for in vivo imaging with BombesinRSense 680 is administration via intravenous injection andimaging 24-48 hours post injection.
 The tissue half-life of BombesinRSense 680 is approximately 48 hours. Repeat injection and imaging may be performed every 7 days forlongitudinal studies. It is recommended that a pre-injection baseline image be taken prior to re-injection and imaging if repeat injections
are to be performed less than1 week apart.
 BombesinRSense 680 enables imaging of HT-29 tumors implanted subcutaneously in nude mice.

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NOTES BombesinRSense 680 is intended for research purposes only and is not for human use. It must be used by or directly under thesupervision of a technically qualified individual experienced in handling potentially hazardous materials. Please read the Material Safety
Data Sheet (MSDS) provided for this product.
 Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents pending. Our products
are not available for resale or other commercial uses without a specific agreement from PerkinElmer.

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