TECHNICAL
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Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

ProSense® 750 FAST IN VIVO FLUORESCENT IMAGING AGENT
Product Number: NEV11171

DESCRIPTION: ProSense® 750 FAST is part of a family of activatable fluorescent imaging agents comprising a novel
architecture termed F.A.S.T. (Fluorescent Activatable Sensor Technology) that confers an improved pharmacokinetic profile
with earlier imaging time points. This architecture offers higher target specific signal with reduced background while also
reducing the optimal imaging time after injection.
ProSense 750 FAST is a protease-activatable, pan-cathepsin fluorescent in vivo imaging agent that is activated by key diseaseassociated proteases such as Cathepsin B, L, S, K, V and D. ProSense 750 FAST is not cleaved by trypsin or plasmin.
Cathepsins are produced by inflammatory and tumor cells. ProSense 750 FAST is optically silent in its un-activated state andbecomes highly fluorescent following protease-mediated activation. ProSense 750 FAST has a half life in plasma of 30
minutes. ProSense 750 FAST may be used to study disease onset and progression in animal models of oncology, inflammation
and atherosclerosis.

MATERIAL (Needs to be reconstituted)

CONTENTS: ProSense 750 FAST: Each vial contains 48
nmol of ProSense 750 FAST in dry solid form. ProSense 750
FAST has been filtered through a 0.2 µm filter prior to drying.
Reconstitute ProSense 750 FAST with 1.2 mL of 1 x PBS
before injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 4 nmol (100 µL) of ProSense 750 FAST per mouse.

Table 1. ProSense 750 FAST Properties
Property
Specification
MW~22,500 g mol-1
Fluorescence1
 Excitation
750 nm Emission
770 nmPurity2>95 %AppearanceBlue Solid1.
2.

Absorbance and fluorescence emission maxima ofProSense 750 FAST in 1 x PBS.
As determined by HPLC and measuring absorbance at 750 nm.

Fig 1.

PROPERTIES: The physical properties of ProSense 750
FAST can be found in Table 1.

STORAGE & HANDLING:


Upon receipt, ProSense 750 FAST should be
IMMEDIATELY STORED AT 2 – 8 ºC ANDPROTECTED FROM LIGHT.
When stored and handled properly, ProSense 750
FAST is stable for up to twelve months.
Before opening the vial check to ensure that all of thesolid material is at the bottom of the vial.
After reconstituting with PBS, gently swirl thesolution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution isstable up to 7 days when stored at 2 – 8 ºC andprotected from light.

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Absorbance (blue) and Emission(red) spectra of ProSense 750 FAST in 1 x PBS

Allow ProSense 750 FAST imaging agent to equilibrate to room temperature before injecting into animals.

IN VIVO IMAGING AND APPLICATIONS:
The recommended procedure for in vivo imaging with ProSense 750 FAST is intravenous administration (for example via tailvein injection) and imaging 6-24 hours post injection.
Imaging in Oncology: ProSense 750 FAST can be used as a marker for tumor progression in animal tumor models.
Optimal signal may be found in 4T1 tumors from 6-24h with extremely high target to background ratios. Washout ofsignal in tissue occurs by 72h.
Imaging in Inflammation: ProSense 750 FAST can be used as a marker for the inflammatory response due toincreased macrophage activity in animal models. In mouse models significantly higher ProSense 750 FAST signal in
the inflamed footpad of DO11.10 mice was found to be associated with the presence of inflammatory cells.

SELECTED REFERENCES:

Weissleder, R., Tung, C.H., Mahmood, U., Bogdanov, A. In vivo imaging of tumors with protease-activated nearinfrared fluorescent probes. Nature Biotechnology 17, 375-378 (1999)

NOTES:
PerkinElmer’s ProSense 750 FAST is intended for research purposes only and is not for human use. It must be usedby or directly under the supervision of a technically qualified individual experienced in handling potentiallyhazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.



Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

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