September 1, 2022
Notice Regarding Start of Sales of Liquid Nitrogen Program Freezers“Cryo Cell Master™ CM-300”
Taiyo Nippon Sanso Corporation (“TNSC”, President: Kenji Nagata) hereby announces thecommercialization and start of sales of the Cryo Cell Master™ CM-300 large-scale liquid nitrogenprogram freezer capable of stably freezing a large volume of cells for cellular medicine manufacturinglines.

1. Background
TNSC, the only liquid nitrogen type cryopreservation container manufacturer in Japan, hascommercialized many related devices and vessels for freezing, preserving and transporting cells up until
now. One of those, a liquid nitrogen type program freezer1 used by many universities, government andcorporate research institutes to prefreeze2 cells, has been delivered in Japan.
Liquid nitrogen type program freezers can prefreeze large volumes of cells at an appropriate freezing rate
because they have higher freezing performance and broader temperature control ranges than electricalprogram freezers.
As regenerative medicine transitions from the research stage to industrial use, TNSC has developed the Cryo
Cell Master™ CM-300, with an internal capacity three times larger than existing products of approximately
300 liters, responding to demand for larger volumes of cell batch prefreezing. The addition of this freezer tothe lineup will respond to a wide variety of customer requirements, from cellular medicine research
applications to prefreezing for pharmaceutical production lines.
*1 Program freezers: Equipment used in prefreezing when freezing cells, and that cancontrol freezing rates as desired.
*2 Prefreeze: A freezing process in which cells are frozen at a controlled freezing rate from room
temperature to about -80℃ to reduce damage to cells.
2. Features of the Cryo Cell Master™ CM-300
This freezer will be the large-size model of TNSC’s CM series standard lineup, and has the following
features:⚫ Easily and stably freeze in line with the freezing program compared to electric types by using the coldheat of liquified nitrogen (-196℃).
⚫ Batch processing of a maximum of 1,575 cryopreservation vials (10 cc); three times more than TNSC’s
existing freezers.
⚫ Equipped with an original freezing program to recreate stable freezing conditions.
⚫ Distribution of chamber temperature at the time of programmed freezing is achieved at a level
equivalent to existing freezers through optimizing the layout of such items as the stirring fan andliquefied nitrogen nozzle.
⚫ Due to its ability to process large volumes at once, compared to the CM-100 existing model capable ofprocessing the same volume, the CM-300 contributes to keeping down capital investment and spacesaving (by occupying an installation area approximately 40% smaller).
⚫ Internal temperatures are quickly recovered following completion of freezing operations through aninternal heater. By shortening the waiting time required until the next process it enables an increase in1

the daily processing frequency.

Chart 1: Comparison between the Liquid Nitrogen Program Freezer and Existing Models
CM-300CM‐100CM‐21BLFeatures(new model)(existing stationary(existingmodel)
tabletop model)
Body dimensions
1,910×810×925
1,010×810×925
535×530×450(W×D×H) [mm]Processing volume for
1,57552525
10 cc
Basicspecifications cryopreservation vials(vials)Power source
Three-phase AC
Three-phase AC
Single-phase AC
200V
200V
100V
3. Future Plans
Cryo Cell Master™ CM-300 sales will start from October 2022.
As the Gas Professionals, TNSC offers customers optimal solutions for gas utilization devices and equipment
as well as contributes to the further development of regenerative medicine by adding to the product lineup
this product, which is expected to be in demand in the manufacturing process of products for regenerative
medicine and more.

Fig. 1 New liquid nitrogen program freezer
Cryo Cell Master™ CM-300

Taiyo Nippon Sanso Corporation
Tnsc.Info@tn-sanso.co.jp

2