Rev Mar 2021

Table of Contents
1. Introduction ..............................................................................................4
2. Set up and Safety .....................................................................................5
3. Software Quick Guides ............................................................................7
Software Icons .........................................................................................7
Software Buttons .....................................................................................8
Software Navigation ................................................................................9Top Bar Overflow Functions ..................................................................10
4. Report and Graph Screens ....................................................................11
5. Basic Operation ......................................................................................14
Best Practices ........................................................................................16
6. Fluorescence: Standard Curve Method Apps .....................................17
7. Basic Fluorometer App ..........................................................................24
Excitation Selection and Emission Results ............................................25
8. Data Export and Print Options ..............................................................26
Export Options .......................................................................................26
Export Via Email ....................................................................................27
Export to a Network Folder ....................................................................28
USB .......................................................................................................30
Print Options ..........................................................................................32
9. Utility Apps and Functions ....................................................................34
Accounts App .........................................................................................34
Data App ................................................................................................36
Export vs Archive ...................................................................................37
Settings App ..........................................................................................39
Updater App ...........................................................................................42
10.Lab Tool Apps ........................................................................................432

Calculator App .......................................................................................43
Timer App ..............................................................................................44
11.Maintenance ...........................................................................................45
12.Diagnostics ............................................................................................46
13.Troubleshooting .....................................................................................47
Quick Help .............................................................................................47
Software Errors ......................................................................................48
14.Customer Support .................................................................................49

3

1. Introduction
Product Technology
DeNovix QFX Fluorometers are compact, stand-alone instruments with a highresolution touchscreen. EasyApps™ Software utilizes application specific icons
along with a custom operating system to provide an intuitive, easy-to-use
instrument for fluorescence measurements.

Key Features include:• Stand-Alone Design, no-PC required• Hi-Definition 7-inch Touchscreen Interface• Simple, Intuitive EasyApps™ Software• Four LED Excitation Options• WiFi, Ethernet and USB connectivity

4

2. Set up and Safety
1. Remove all packing materials including the protective cover on the screen.
2. Plug the unit into a 100-240 VAC, 50-60/Hz receptacle using the grounded
power supply (CUI MPN: ETSA120330U-P5P-SZ, Transformer Dest Top 12V 40W,
Lead free / RoHS Compliant) included with each unit.
3. For instruments with red LED power indicators, turn on the power switch
located in the back of the instrument. For instruments with green LED power
indicators, press and release the power switch.
The Android-based operating system will boot up in less than a minute.
4. Use the Shut Down icon (accessed from within each app or on the Home
screen) to do a soft power down. For instruments with green LED power
indicators, toggle the power switch to restart the instrument.

Cautions
DO NOT REMOVE COVER
No operator serviceable components inside. Refer servicing to qualified
personnel.
The QFX is designed for indoor use under the following conditions:Temperature: 15˚ to 35˚ C
Humidity:
35 to 65 %Use only the power supply provided with the instrument. Use of the
instrument in any manner not specified by the manufacturer may impair theprotection provided by the supplied power cord and power supply.
NE PAS OUVRIR L’APPAREIL.
Aucun composant réparable par un utilisateur est inclus. Confiez l'entretien à dupersonnel qualifié .
Le QFX est conçu pour une utilisation en intérieur dans les conditions suivantes :Température : 15° à 35˚C

5Installation and Safety

Humidité: ........35 à 65%
Utilisez uniquement le bloc d'alimentation inclus avec l'instrument. Utiliser l’appareil dansdes conditions non spécifiées par le fabricant peut compromettre la protection offerte parle cable d’alimentation et le bloc d'alimentation fournis avec l’appareil.

VCCI (Class B) compliance statement for users in Japan:
This is a Class B product based on the standard of the Voluntary Control Council
for Interference (VCCI) from Information Technology Equipment. If this is usednear a radio or television receiver in a domestic environment, it may cause radio
interference. Install and use the equipment according to the instruction manual.
This equipment has been tested for radio frequency emissions and has beenverified to meet CISPR 22.2 Class B.

Instrument Rear Panel
The QFX comes equipped with one Ethernet and three USB ports. The Ethernet
feature is provided for internet connectivity only. The USB ports are used tofacilitate data transfer to a FAT32 formatted USB storage device. In addition, theUSB ports support the use of selected DYMO printers* and an Opticon OPI 3601
USB barcode reader. USB mouse and keyboard devices may also be used with
the QFX.
Please refer to www.denovix for a list of supported Dymo printers.

6

3. Software Quick Guides
Software Icons
Add

Select All

ExportDeleteDeselect All

Sort

Edit

Copy

Info

Undo

Paste

Shut Down

The Overflow (top action bar) is used to access secondary features
such as Select Units, Screen Capture, User Guide and Exit.
Busy icons and progress bars are used to indicate the instrument isin the process of making a measurement. Please wait until the icon
has disappeared before tapping the screen to perform additional
actions.
The USB Flash Drive Eject icon appears in the bottom notification bar
when a drive is detected. The software will automatically safely
unmount the flash drive after a screen capture or a report is exported
to the drive. Tap the icon to remove a flash drive when nothing wasexported or saved.
The Quick Print icon is only displayed on Report or Graph screens
when a DYMO label printer is connected to the instrument.
Auto Export status icons are displayed at the top right of a Run
screen. Ensure WiFi is connected prior to making a measurement.

7

Quick Guides

Software Buttons
The fluorescence apps Standards Measure button is used tomeasure standards when building a standard curve.
The Samples Measure button is used to quantitate samples.
The fluorescence apps Replicates On/Off button is used to define if
the standards curve enables one or up to three replicates.
The fluorescence apps Basic Measure button is used to makesingle measurements not being read from a standard curve.

Fields with Drop-Down Menus are indicated by black or white
triangles located on the bottom right.
Fields with black underlines are editable by the user. Tapping on thefield will enable a keyboard, a numeric keypad or a dialog box.
Application specific editor options can be accessed using the List
Add/Edit button found to the right of some drop-down menus.
Options buttons are used for such purposes as change units ortrend line analysis parameters in the fluorescence apps.
An accept and return to previous screen button is found in the lower
right of some screens. All changes made to a selection are
immediate.

8Quick Guides

Software Navigation
Apps are launched using Home screen icons. Swipe the Home
page to the left for additional icons.
The app Run, Report and Graph screens are accessed either
by the tabs on the top bar or by swiping the screens left or
right.
Use the Current Account Tab drop-down menu on the top
action bar in the Run, Report and Graph screens to select a
specific account within an app. Use the Accounts app tocreate and manage accounts.
The bottom bar Back button is used to navigate to the mainlevel of the current app.
The bottom bar Home button is used to navigate to the Home
screen without closing the app.
The bottom bar Recents button is used to as a means ofdisplaying recently used apps. Swipe the app to the left fromthe Recents list will close the app.
The bottom bar Collapse button is used in place of the Backbutton whenever a keyboard is enabled. Use this button todismiss the keyboard.

Notification Bar
Information such as available updates, scheduled system backups reminders
and WiFi connectivity status are located on the bottom action bar adjacent to thedate and time display. Swipe the notification up for details. Tap outside thenotification box to minimize the information.

9

Quick Guides

Top Bar Overflow Functions

10

ScreenCaptureA .jpg may be exported as described in the chapter entitled"Screen Capture and Export" using this option.

ExportSelected
Samples

A .csv file of sample data may be may be exported as
described in the chapter entitled "Screen Capture andExport". Network printing is also enabled using this option.

ReportFormats

Customized report export formats are configured using thisoption.

Archive
Selected
Samples

The Archive function exports data as a .csv file to a network
folder or USB flash drive and permanently deletes it from the
instrument.

Clear
SamplesThis function saves and then clears all current sample
records from the Report and Graph screens.

User Guide

The User Guide may be accessed either from the Overflow
icon within any app or by launching the User Guide app.

About

The About feature brings up an information box displaying
the current app version number.

Exit

Use the Exit function to close an application.

4. Report and Graph Screens
The measurement apps generally use a three page structure consisting of theRun, Report and Graph screens. The various screens are accessed either byusing the top tabs or by swiping the touch screen left or right.
The Run screens for each type of assay will be described in the specific app
chapters.

Report Screen• The Report screen list includes application specific data fields for eachsample measured in the current measurement session.
• Tap rows to highlight and select data for export. The Select All /UnSelect All
icons are used to quickly select or deselect all rows.
• The change will be applied to the current Report and Graph screen rows aswell as to the sample information retrieved using the Data app.

11

Report and Graph Screens• Clear highlighted sample records from the both the Report and Graph screensusing the Delete icon. The data is removed from the current report but is stillavailable using the Data app.
• Highlighted sample data may be exported or printed using the top action bar
Overflow Screen Capture or Export Selected functions. Additional information
is provided in the chapter entitled "Screen Capture and Export".

Graph Screen• Tap rows to highlight and select data to display the spectra. The Select All /UnSelect All icons are used to quickly select or deselect all rows.
• Up to 24 samples may be overlaid on the graph. The data will be presented as
the concentration calculated from the standard curve trend line- not the
sample tock concentration which takes dilution factors in consideration.
• Color blocks are used as a legend convention.

12

Report and Graph Screens• Highlight a single row and then tap the Edit icon to add or modify a sample
name. The change will be applied to the Report and Graph screen rows.
• Clear highlighted sample records from the both the Report and Graph screensusing the Delete icon. The cleared data will be available using the Data app.
• Highlighted sample data may be exported or printed using the Overflow
Screen Capture or Export Selected functions. The data will will exported asa .csv file . Additional information is provided in the chapter entitled "Screen
Capture and Export".

13

5. Basic Operation
DeNovix Fluorometer software includes fluorescence EasyApps™ Software. The
fluorescence app icons will appear on the Home screen.
The table below lists the four LEDs included in the QFX. Users must select theappropriate LED for the assay that they choose for quantification.

LEDs

Excitation Filter Range

Emission Filter Range

UV361-389 nm

435-485 nm

Blue442-497 nm

514-567 nm

Green490-558 nm

565-650 nm

Red613-662 nm

665-740 nm

Fluorescence Measurements
1. Launch the app of interest from the Home screen.
2. Insert a micro tube pre-filled with the fluorescent solution. Close the coverand tap Measure.
Notes:• Use clear thin-walled 0.5 mL plastic PCR tubes only
• Measure 200 µl volume samples.

14

Basic Operation

Screen Saver• The backlight for the screen will automatically turn off after 10 minutes ofinactivity. Simply tap anywhere on the screen to resume the last function.
• Although the screen is turned off for the screen saver mode, functions suchas the Timer continue in the background. Always check that these apps are
not in use in the background prior to turning off the instrument.

Mechanism Busy Indicator• A progress bar will be displayed on the screen when the measurement
mechanism is busy.
• Please do not lift the arm, push software buttons or turn the instrument off
while a busy indicator is displayed.

Measurement Sessions• New measurement sessions begin each time an app is accessed from aclosed state or when the account is changed while the application is open.
• In addition, new sessions automatically begin when the number of sample
records on the Report screen reaches 9999.

15

Basic Operation

Best Practices• Ensure all sample concentrations in the thin walled assay tubes fall within thelimits of the assay or reagent kit for accurate results.
• Use 200 μL volumes in a clear thin walled PCR tube.
• Lower the cover before tapping the measure button.
• Avoid introducing air bubbles into the sample solution when mixing samples.
• Follow assay reagent manufacturers' recommendations regarding
temperature, incubation time, and protection from ambient light.
• Follow assay reagent manufacturers' recommendations regarding suggested
standard curve concentrations and data analysis.
• Ensure all samples and standards are treated identically in terms of
incubation times and temperature.
• Minimize assay tube and solution temperature fluctuations as these may
impact the accuracy of the assay. Samples at higher temperatures will
generally have lower fluorescence.
• Do not label the side of an assay tube as this could interfere with the sample
measurement.

16

6. Fluorescence: Standard Curve Method Apps
The EasyApps™ listed below are designed using the same basic architecture.
Each app includes a choice of four excitation LEDs (UV, Blue, Red, Green) and avariety of assay. The end user is responsible for selecting the proper choicebased on the analyte that will be used for quantification.

FluorodsDNA•DeNovix dsDNA Broad Range•DeNovix dsDNA High Sensitivity•DeNovix dsDNA Ultra High Sensitivity•Quant-iT™ PicoGreen•Quant-iT™ dsDNA HS•Quant-iT™ dsDNA BR

FluoroRNA•DeNovix RNA Assay•Quant-iT™ RiboGreen•Quant-iT™ RNA HS•Quant-iT™ RNA BR•Qubit™ RNA HS

FluorossDNA•Quant-iT™ OliGreen•Qubit™ ssDNA•Quantifluor ssDNA

FluoroProtein•Quant-iT™ Protein•Qubit™ Protein•Qubit™ dsDNA HS•Qubit™ dsDNA BR•Quantifluor dsDNA•Quantifluor One
dsDNA•Qubit™ RNA BR•Quantifluor RNA•Quant-iT™ microRNA•Qubit™ microRNA

Fluoro•All entries to be defined by the user
Std Assays

17

Standard Curve Methods

Quick Protocol
1. Launch the app from the Home screen.
2. Select the LED using the drop-down menu on the Run screen.
3. Generate a new curve:•

Insert the standard (200 uL in a thin walled PCR tube) into the sample
holder and lower the cover. Measure at least one replicate for aminimum of 2 standard concentrations•

Alternatively, import a saved curve or list of sample concentrations

4. Tap the Samples radio button. Enter a sample name.
• Optional: Enter a dilution factor to be applied to the reported
concentration.
5. Quantitate a sample using the Measure button.

Refer to the manufacturer of the assay reagent for assay specific instructions.

Note:•DeNovix and Qubit assays will have predefined standard concentrations and specified
units.

18

Standard Curve Methods

Run Screen- Assay and Curve Selections
Excitation Source (LED) and Assay Selection• Select an LED from the drop-down menu in the left panel. The user mustchoose the proper LED for the analyte in use (SW V3.52 and later).
• New assays may be entered using the List Add/Edit button.
• User defined assays may be used by all accounts but may be modified ordeleted only by the account owner.

Curve Options
Generate New Curve• Choosing this option will pop-up a dialog window used to enter theconcentration values for the standards.
• Verify the concentrations of the standards for the LED / Assay chosen. The
standard concentrations for all other assays are determined by the user at thetime of the assay.
• Trend line analysis options and concentration units used may be changed
using the advanced options button.
• Blank: If selected, the first row in the standards table is used to measure aBlank.The average RFU value of the blank measurements will be
subtracted from all standard and sample measurements. If the Blank is
not selected, the initial concentration for preconfigured assays will be set
to 0.00. This standard is typically the reagent/fluorophore solution without
any nucleic acid or protein.
• Trend Line: If selected, the trend line will be forced through the origin(0,0).

19

Standard Curve Methods

Use Previous Curve or Std Values• Choosing this option will pop-up a dialog window with a radio button selector
for saved standard curves and saved standard curve concentration lists.
• All modifications to a curve or list are effective immediately. It is important tosave the selection with a new name prior to making any changes if you do notwant to modify the original curve or standards list. Changes to a curve do notaffect previously measured samples.
• Although all saved lists and saved curves are available for use by all accounts,
only the account holder may modify the selection.

20

Standard Curve Methods

Run Screen- Standard Curve View

• The Stds button will pop-up the Edit Concentrations for Standard Curve
window.
• The Delete and Undo icons are used to manage the RFU values within thetable.
• The Save button will pop-up the Save Values window. Either concentration
values or concentration values with the associated RFU values may be saved.
• The New Curve button is used to generate a new curve or load a previously
saved curve. All sample data generated using the Samples radio button will
be automatically saved and accessible using the Data app.
• The Replicates arrow button enables the use of up to three replicate
measurements per standard concentration. The average of the replicates will
be used to generate the trend line.

21

Standard Curve Methods

Run Screen- Sample Results View

• Use the Samples radio button to display the measurement results view.
• The samples Measure button will be disabled until one replicate of at least
two standards are measured using the standard curve view.
• The Concentration from Curve results are calculated directly from thestandard curve trend line. Results are reported in terms of the units used toderive the trend line.
• The Optional Dilution Factor (or the µl Added value for Qubit™ assays) will beapplied as a multiplier to the Concentration from Curve value and the result
reported in the Sample Stock Concentration field.
• The Sample Stock Concentration results are reported in terms of the units
selected by the user. The value saved in Report will be displayed in terms of
the units in use at the time of the measurement.

22

Standard Curve Methods

New Standard Curve Assays/Methods
Each new method requires a unique name. In addition, the following parametersmust be defined:

Excitation Source• Use LED Radio buttons to select the excitation source.
• The LED Wizard may be used to determine a compatible excitation source
if not known. The software will make a measurement using each of thefour excitation sources and will report the associated RFU in the colorcoded boxes• Some fluorophores do not exhibit significant fluorescence unless boundto a protein or nucleic acid. It may be necessary to set up an assay
reaction to best use the LED Wizard.
• The standard curve assay methods use one excitation source todetermine the concentration of a sample based upon the RFU measured
using the corresponding emission filter set.

Advanced Options• Use the Advanced Option buttons to set both the Trend Line/Blank
selections as well as the standard curve units.
Although all accounts may use any saved method, only the method accountholder or an administrator may modify or delete the new method.

23

7. Basic Fluorometer App

Basic Fluorometer

This app enables the QFX instrument to be used as a versatile fluorometer for
applications such as dye QC checks and assay development. The app is
especially useful for fluorophores with significant Stoke shifts as it allows theuser to excite a sample with one LED and get RFU results from multiple emission
channels.

Quick Protocol
1. Launch the app from the Home screen.
2. Select an excitation LED using the radio buttons.
3. Insert the standard (200 μL in a thin-walled PCR tube) into the FX mode
sample holder.
4. Optional: Create and Save a used defined method (LED selection and user
defined assay name).
5. Optional: Enter a sample name.
6. Tap the Measure button.

24

Basic Fluorometer

Excitation Selection and Emission Results• Select an excitation source (LED) or LED plus fluorophore combination.
• The selected LED is the only excitation source used during a measurement.
However, the emission RFU will be collected for each longer wavelength
emission filter set.
• For example, if the blue LED is selected, the the emission results for theblue, green and red channels will be reported. If the green LED is selected,
only the green and red channel results will be reported.
• New fluorophores may be entered using the List Add/Edit button.
• The Add button will pop-up a window that includes a LED Wizard. Tapping
the Wizard button will result in the sample being excited by each LED and
the RFU measured by the corresponding detector. The LED that resulted in
the largest RFU at its detector will automatically be chosen as the LED forthe method.

25

8. Data Export and Print Options
Export Options
The data associated with highlighted samples on a Report or Graph screen may
be exported via email, sent to a network folder or saved to a FAT32 formatted
USB flash drive as .csv files.
• Data may be automatically exported to a network folder after every
measurement. Refer to the "Network Folder Set-up" section for more
information.
• To open an exported .csv file on a computer, the DeNovix instrument and thecomputer must both be set to use either period or comma number formats.
The QFX number format is determined by the language selected using theSettings app.

Export Formats
Standard Formats• The default .csv file format for each absorbance measurement app includes
all absorbance vs wavelength data for the app specific measurement
wavelength range as well as the calculated data displayed on the app Report
screen.
Custom Formats• User specific formats may be defined using the Export Formats option
accessed from the Report screen Overflow menu.
• The format used when exporting results is selected at the time of the export.
Accounts other than the General Account may set a user-defined format as
the default selection using the Accounts app.

26

Export and Print

Defining a New Format:• Provide a title for the format.
• Hold the arrow icon and drag a column up or down to change the order of thecolumns.
• Touch on the column name and swipe it left or right to exclude or include it in
the exported report.
• Tap on the input field to change the name of a column header in the exported
report.

Graph Screen Exports• Selected sample results exported from an absorbance app Graph screen
follow the same format as when exported for the Report screen. Graphs are
not exported as part of the .csv file.
• Use the Screen Capture option to export the current graph via email or to savethe .jpg image to a network folder or a FAT32 formatted USB flash.

Export Via Email• Screen captures as well as data .csv files may be exported via email.
• Use the Account app Overflow Address Book function to save frequently used
recipient email addresses.
• It is recommended that a new, dedicated POP3 Outlook account be used forall outgoing email from the QFX. This type of account works very well with the
instrument's operating system and prevents personal incoming email frombeing stored on the unit.

27

Export and Print

Email Set-up
1. Ensure that the instrument is connected to WiFi or Ethernet.
2. Swipe the Home screen to the left to navigate to the second page, launch the
Email app and follow the prompts using the Manual setup option.
3. Input Incoming Server Information:• Select the POP3 option on the Account Types screen. Do not use an IMAP
account as messages from IMAP accounts could occupy a significant
amount of the instrument's memory.
4. Set the inbox checking frequency to "Never" on the Account Options screen.
The QFX email should be used only to export data and screen captures.
5. Input Outgoing Server Information:• Use settings appropriate for the lab email server.
6. Enter a name to display on outgoing messages.
Refer to your local IT support group if additional assistance is required.

Export to a Network Folder• Screen captures, data report .csv files, as well as instrument backups anddata archive files may be sent to a selected network folder.
• The two supported transfer options include Server Message Block (SMB) andFile Transfer Protocol (FTP).
• Use the following apps to manage network folder options on the QFX:

28

Export and Print

Settings App• Permission to setup a network folder for the General Account as well as
standard user accounts are enabled or disabled by an administrator using
the Permissions option in the Settings app. "Enabled" is the default
selection.
Accounts App• Network folder paths are defined and saved on a per user account basis
from the Accounts app. The primary Administrator network folders areused only for the Backup and Restore function in the Settings app.

Network Set-up
1. Contact your local IT group to confirm that you have access to a local server
or FTP site. Ensure that the instrument is connected to WiFi or Ethernet.
2. Launch the Accounts app and select a user account.
3. Use the top drop-down menu in the right pane to select the Add New
Network Folder function.
4. Use the List Add/Edit button to add a network folder to the drop-down menu.
5. Select either the SMB or FTP option.
6. Enter a nickname for the network path. This is the name displayed in all
drop-down menus referencing the network folder.
7. Enter the server computer's host name. Contact your local IT group for
additional information.
8. Select log-in option. Contact your local IT group to request log-in credentials.
• Log in: Enter the network folder username and password required toaccess the selected network folder.

29

Export and Print• Anonymous: No user name or password required.
• File (SMB only): Upload a configuration file (file extension .prp ) from a
USB device. Note: If a username or password is entered that is different
from information contained in the config file, the manually typed
information will be used in validating the path access.Refer to http://
jcifs.samba.org/src/docs/api/overview-summary.html#scp for additional
guidance.
9. Use the Browse button to locate and select a specific folder path.
10. Use the Auto Report checkbox to enable automatic export of sample data
after every measurement.

USB• Screen captures as well as data .csv files may be saved to a FAT32 formatted
USB flash drive.
• The USB Flash Drive Eject icon appears in the bottom notification bar when adrive is detected. The software will automatically safely unmount the flash
drive after anything is written to the drive. If not removed, the software willautomatically remount the flash drive when another write operation is
performed.
• Please wait for the “OK to remove” toast message to appear before
removing the drive.Tap the icon to safely remove a flash drive whennothing was exported or saved. Failure to follow these instructions may
result in the flash drive becoming unrecognizable to the instrument.
• There are three USB ports on the back of the instrument although only one
storage device is recognized at a time. The two additional ports may be usedfor DYMO printers and barcode readers.

30Export and Print

Auto Export Icon
When the auto export feature is selected, a status icon will appear at the top
right of the screen after the first measurement.

Export in Progress

Export CompletedExport Failure• Automatic export of every sample measurement to a network folder is enabled
on a per user account basis.
• The auto export feature may only be selected for one saved network folder peruser account.
• Automatic exports will be stored in a single file per app type per user accountper day.
• When selected, the auto export will apply to all measurement apps used by theapplicable account.
Note: All sample data is saved to the instrument even if automatic exports faildue to server connectivity issues.

Troubleshooting Tips
• Confirm that there is WiFi or Ethernet connectivity. Use the Browser app on thesecond page of the Home screen to confirm connectivity or check theconnectivity status icon on the right of the bottom navigation bar.
• Confirm that the log in credentials are valid for the network folder option
selected and that the hostname is correct.
• For internet connectivity issues, network folder access failures and all otherserver related issues, contact your local IT support group.

31

Export and Print

Print Options
The QFX software supports printing to networked printers or to selected DYMO
printers*. All Dymo print-outs are in black and white.
*Please refer to www.denovix for a list of supported Dymo printers.

WiFi and Networked Printers
Screen shots as well as selected sample data and graphs may be printed to both
Wifi enabled and networked printers.
• Use the Overflow menu to select either Screen Capture or Export Selected
Samples to bring up a dialog box with Network Printer as a radio button
option.
• Selecting this option will display a preview of the print-out along with manycustomizable options.
• To select a printer, ensure that the instrument has WiFi or Ethernet
connection and then tap the first item listed under Printer Options. This willbring up a list of available printers. If no printers are listed, tap the Network
Printers icon in the left pane to search for nearby printers.
The current software supports approximately 90% of printers on the market.
DeNovix will continue to add new drivers to accommodate as many new printers
as possible in future software updates but cannot guarantee compatibility withall printers.
Please contact DeNovix or your local distributor for a list of recommended
printers if your current printer is not compatible with the software.

32

Export and Print

DYMO Printers• The software enables the use of three DYMO paper or label sizes:• 4x6 labels (DYMO 4XL only) and 2.5 inch paper rolls are used to print
screen captures and selected sample results from the Report or Graph
screens.
oReport and Graph print column formats will be as shown on the
instrument display.

• 1 x 0.5 inch labels are used to print sample names, stock sample
concentrations, units and the date on individual labels suitable forstandard microfuge tubes.
• For the basic fluorometer app- the label will include the excitation LED
and the associated channel RFU value.

• A Quick Print button will be displayed on the Run, Report and Graph screens
when a DYMO printer is detected. Label sizes may be selected by tapping theprinter icon displayed next to the date and time in the bottom notification bar.
• Although sample names may include up to 48 characters, the DYMO printed
label will print 2 rows of characters per sample name. The exact number ofsample name characters may vary depending on the language in use.

33

9. Utility Apps and Functions

Accounts

Data

Settings

Updater

Accounts App
User accounts manage default settings within specific apps as well as saved
sample and dye types, wavelength lists and user methods. Accounts are also
used as a means of segregating data when performing searches in the Data app.
The Add New Network Folder function is accessed from a drop-down menu on
the right side of the screen. The Address Book function accessed from theOverflow menu in this app allows users to save frequently used recipient email
addresses for easy reference.

System Accounts
There are two preconfigured system accounts built into the software:• Primary Administrator• This account is used to manage instrument settings. It is not used within
the measurement applications.
• The primary administrator account is not initially password protected. A
password may be applied at any time. Contact DeNovix if help is required
to reset the primary administrator account password.
• General Account• This account is used as the default selection for all apps each time an app
is opened. This account may not be password protected or deleted.

34

Utility Apps

User Added Accounts
All added administrator or standard user accounts may be password protected.
• Administrator Accounts• An administrator level account may only be created by anotheradministrator.
• Administrator accounts may modify or delete user entries of any sortcreated from within any account.
• Administrators may change the password for any account as well as
delete any account.
• Standard Accounts• Any user may add a standard level account.
• Standard accounts may only modify or delete sample or dye types,
wavelength lists or user methods created from within their account.
If an account is deleted, all associated sample and dye types, wavelength lists
and user methods are still available for general use unless deleted by anadministrator.
Contact the holder of an administrator account if a standard user accountpassword is forgotten. Contact DeNovix customer support or your local
distributor if the Primary Administrator password is forgotten. Please have theserial number of the instrument available when requesting help for the Primary
Administrator password reset.

App Default Selections
Some application specific default settings may be defined on a per accountbasis. Launch the Accounts app from the Home screen and then use the dropdown menus to select the account, the app and the setting of interest.
Note: The default selections for the General Account may not be modified.

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Utility Apps

Data App
This app allows the user to search for past data using either a Advanced Searchor Quick Search option. Data can then be added to the Report and Graph screens
for review and export. In addition, data may be archived from the Search Screen.

Search Options• Data is automatically filtered based upon the Account selected using the tabon the top action bar.
• The Advanced Search allows multiple filters to be applied in a single search.
The Quick Search option enables the user to filter by a single criteria in
addition to the automatic Account filter.
• Diagnostics results are not accessible using Quick Search.
• Once the filter criteria have been selected, tap the Search button at the bottomof the screen. Tap any column header to sort the results list.
• Highlight the sample(s) of interest and tap the Add to Report button to include
the samples on the Report and Graph screens. Blank measurements may be
included in the list but will not provide any absorbance data.

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Utility Apps

• All data must be from the same app type for the samples to be added to theReport or Graph screens.
• Note: The Data app does not include a Graph screen for fluorescence
apps.

Export vs Archive• All data is automatically saved to the QFX onboard computer database at thetime of measurement.
• The Auto Export feature is available when a network folder is set-up using
the Accounts app.
• The Export Selected Samples function available from the Report and Graph
screen overflow menus creates a .csv that can be emailed, sent to a network
folder or saved to a FAT32 formatted USB flash drive.
• When the export function is used, the data is not deleted from thedatabase and can be accessed using the Data app.

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Utility Apps

• The Archive Selected Samples function available from the Run and Report
screen overflow menus creates a .csv file with sample data that can beexported to a folder entitled ds11 on a FAT32 formatted USB flash drive or
saved to a network folder.
• When the archive function is used, the data is permanently deleted fromthe instrument database and cannot be restored to the instrument.
• The archiving privilege for standard accounts is enabled or disabled using theSettings app. When disabled, only administrative accounts may archive data.
• The storage capability of the QFX is sufficient for several years of data for alab with average use. Therefore, it is recommended that the archiving function
be used only as an infrequent method of deleting old data from the QFX
database. Although archiving a large amount of data may take a considerable
amount of time, the archive process continues to work even when theinterface goes to sleep after 10 minutes of no user activity. Touch the screen
to wake-up the user interface and update the progress message.
• Use the Cancel button to stop the archive button. Samples already copied up
to the stop point may be deleted from the instrument at the user's discretion.

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Utility Apps

Settings App
The Settings app may be found on the second Home screen page. Only
administrator accounts may access and change settings.
The app provides access to the following functions:

Backup and Restore• The backup process exports a copy of all user-defined app settings, methods,
dyes, sample types, email addresses, shared folder paths, user accounts andsample results to either a USB flash drive or a network folder.
• Backup files include a DeNovix specific extension and may not be opened on
a computer. The files may be restored back onto the QFX at any time.
• Automatic backup reminders may be enabled using the checkbox on the
Backup screen.
• The restore function is used to recover user defined app settings, methods,
dyes, sample types, email addresses, shared folder paths, user accounts andall sample results saved at the time of the backup. There are two options:• Recover All: Restores user defined app settings, user entered methods,
user added dyes and sample types, email addresses, shared folder paths
and saved user accounts as well as previous sample results. All new
methods, sample and dye types, emails addresses, network folders, user
app settings and data results accumulated since the time of the back-up
will be overwritten during the restore process.
• Recover User Info Only: Use this option to recover settings, methods,
dyes, sample types, email addresses, shared folder paths and user
accounts. This option will not restore sample data or overwrite new data.

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Utility Apps

Date/Time• Select manual or automatic date and time options.

Language• Although the Android system provides translations for some routine
functions in many languages, the majority of the app specific text will beavailable in a limited number of language options.
• The QFX number format is determined by the language selected.
• Exit all apps before changing languages.

Network Information• The WiFi and Ethernet media access control addresses can be foundunder this setting.

Permissions• This selection is used to enable the following options:
Archive: Use this selection to enable or disable all standard accounts,
including the General Account, to archive (delete) data from the
instrument. Refer to the Export vs Archive section for additional
information regarding archiving.
Automatic Updates: Use this selection to enable or disable automatic
updates of the software.
Network Folder: Use this selection to enable or disable the generalaccount and/or standard user accounts to set-up network folders fordata export/archive and system backups.

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Utility AppsSound
• Volume controls are available for media and timer alarms.

Storage• Displays storage statistics.

WiFi
To enter static IP addresses :1. Ensure the WiFi is on. Select WiFi from the Settings list.
2. Tap the Add icon on the right side of the top action bar.
3. Dismiss the keyboard using the chevron on the bottom action bar.
4. Select the Show Advanced Options checkbox.
5. Select Static using the IP settings drop-down.
6. Enter the appropriate information and tap Save.

Ethernet
To enter static IP addresses :1. Ensure the Ethernet cable is connected. Select Ethernet from theSettings list.
2. Tap the name of the connection. Use the drop-down menu to selectStatic. Enter the appropriate information and tap Apply.
Note: WiFi will take precedence over the Ethernet when both are connected.
Contact your local IT department if additional help setting up WiFi or Ethernet
static IP addresses is needed.

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Utility Apps

Updater App
This app is used to update all apps to the most current available versions. The
latest updates are available at www.denovix.com. For instruments without
internet access, a zip file with the latest updates may be downloaded from thewebsite onto a FAT32 formatted USB flash drive.

Procedure
If the QFX is connected to the internet via WiFi or Ethernet proceed to step 3.
1. Download the required package from the www.denovix.com website directly tothe root level of the flash drive. Do not unzip the file folders.
2. Shut down all open apps.
3. Swipe to page 2 of the Home screen and launch the Updater app.
4. Click OK if a message is displayed that indicates new firmware is required. Do
not turn off the power to the instrument during the 10- to 15 second firmware
update process.
Note: Some installed apps are used for important background system functions
and will not be visible on the Home screen.

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10.Lab Tool Apps

Calculators

Timer

Calculator App
Three calculators are included within the app. Use the tabs in the top action bar
to navigate between the options.

Oligo Calculator
This calculator analyzes a user-inputed ssDNA or RNA sequence of interest.
• Outputs include:• Length• Molecular Weight (g/mol)• GC Content• Mass Extinction Coefficient (µg/OD @260 nm): This result may be copiedand pasted into the Factor Input field used for nucleic acid concentration
calculations in both the ssDNA and the Microarray apps.
• Melting Temp: For sequences up to 13 nucleotides, the equation used is
Tm= (wA+xT) * 2 + (yG+zC) * 4 where w,x,y,z are the number of the bases
A,T,G,C in the sequence. For sequences longer than 13 nucleotides, theequation used is Tm= 64.9 +41*(yG+zC-16.4)/(wA+xT+yG+zC). Bothequations assume that the annealing occurs under the standard
conditions of 50 nM primer, 50 mM Na+, and pH 7.0.

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Lab Tools

Scientific Calculator
Use this calculator for standard mathematical and scientific functions.

Dilution Calculator
This calculator is a useful tool to calculate how much of a stock solution should
be added to a buffer solution to result in a final specified solution concentration.
• Both molar and mass units are available. However, conversions between the
two types of units are not enabled.

Timer App
This app is a lab tool that allows a user to set two independent timers.
• At the end of a specified period, an alarm will sound for a maximum of 90
seconds.
• Push the Off button to silence the alarm.
• The alarm can be acknowledged via the notification bar or by going back to
the Timer app.
• The Timer will work in the background while other measurement apps are in
use as well as when the screen is in the dark screen saver mode.

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11. Maintenance
Guidelines• Use only a dry, soft, lint-free cloth to clean the front screen.
• Do not use a spray bottle to apply water or any other solutions onto any
surface of the instrument as the liquid may damage internal components.

ScreenLocker App
Launch the ScreenLocker app from page 2 of the Home screen prior to cleaning
the touch screen. This will disable swiping motions from activating othersoftware activities. Tap the Droid icon three times to unlock the screen.

Fluorescence Mode Holder Cleaning
The tube holders may be cleaned of excess dust using canned air or a damp (notwet) lint free cotton swab.
• Do not allow any liquid to drip into the holders as it may damage the internal
components of the instrument.

Customer Support
Contact DeNovix Customer Support at 1-302-442-6911 or info@denovix.com if
additional help is required. Please include the serial number of your instrument
when contacting Customer Support by email.
Outside of the US, please contact your local distributor for assistance.

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12. Diagnostics

Fluorometer Diagnostics

The Fluor Diagnostics app is used to confirm that the LED excitation sourcesand detectors are functioning properly.

Procedure
1. Ensure sample measurement tube holder is empty and the lid is closed
2. Launch the FX Diagnostics app and push the Start button.
• The software tests that each LED is firing and each detector is functioning
properly. Results will be reported as Pass/Fail.

Technical Support
Contact DeNovix Customer Support if additional help is required. For US
customers, please email the results directly to info@denovix.com using the
Diagnostics app Overflow Tech Support function. Include your name, phone
number, institute/company, and return email address. Please note responses will
not be sent directly to the instrument email account.
The Tech Support email includes encrypted files that can only be opened by
DeNovix technical support team.
Outside of the US, please contact your local distributor for assistance.

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13. Troubleshooting
Quick Help
Most issues concerning accuracy and reproducibility are sample or techniquerelated and are resolved by following the suggestions below:• Ensure all solutions are homogenous and well-mixed prior to sampling.
• In the case of fluorescence quantitation assays, ensure sample
concentrations fall within the reagent kit limits as described by the reagent
manufacturer.
Fluorescence Mode
• Use 200 μL volumes in a clear thin walled PCR tube
• Avoid introducing air bubbles into the sample solution when mixing samples.
• Ensure all sample concentrations in the thin walled assay tubes fall within thelimits of the assay or reagent kit for accurate results.
• Follow assay reagent manufacturers' recommendations regarding
temperature, incubation time, and protection from ambient light.
• Follow assay reagent manufacturers' recommendations regarding suggested
standard curve concentrations and data analysis.
• Confirm that the correct LED excitation source was used.
• Confirm that standard concentrations and dilutions were performed correctly.
• Confirm that the correct concentration units for the standard curve and theunknown samples were used to calculate the stock concentrations.

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Troubleshooting• Ensure that the correct optional dilution factor (or uL added in the case of
Qubit® assays) is entered into the appropriate Run screen field before ameasurement is made.
• Ensure all samples and standards are treated identically in terms of
incubation times and temperature.
• Minimize assay tube and solution temperature fluctuations as these may
impact the accuracy of the measurement.
• Do not label the side of an assay tube as this could interfere with the sample
measurement.
Additional troubleshooting resources (technical notes and FAQs) are available atwww.denovix.com.

Software Errors
Pop-up messages will provide guidance for software-based errors or invalid user
actions. In most cases, simply relaunching the app will allow the user tocontinue to make measurements. Update the software using the Updater app if
experiencing a reoccurring software error.
Contact DeNovix Customer Support 302-442-6911 or info@denovix.com if
additional help is required. Please include the serial number of your instrument
when contacting Customer Support by email. The serial number may be foundon the bottom of the instrument.
Outside of the US, please contact your local distributor for assistance.

Printer and Barcode Scanner
The QFX is compatible with selected DYMO printers* and Opticon OPI 3601 USB
barcode scanners. For accessory product troubleshooting and warranty
information, please contact the manufacturer of the product. DeNovix support islimited to the specific use of the product with a QFX instrument.
*Please refer to www.denovix for a list of supported Dymo printers.

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14. Customer Support
DeNovix Inc.
3411 Silverside Road, Hanby Building
Wilmington, DE USA 19810
302-442-6911
info@denovix.com
www.denovix.com
Please include the serial number of your instrument when contacting Customer
Support by email. If sending Customer Support a Screen Capture or a report .csv
file using the QFX email feature, include your name, institute/company, phone
number and return email address. Please note responses will not be sent
directly to the email account used by the instrument. Outside of the US, pleasecontact your local distributor for assistance.

Warranty
All product(s) and accessories sold by DeNovix Inc. are under warranty against
manufacturing defects in parts.

Disclaimer
All information in this document is for reference purposes only. DeNovix Inc.
makes no claims that this document is complete or error-free and assumes noresponsibility and will not be liable for any errors, omissions, damage or loss
that might result from any use of this document.

Research Use Only
DeNovix Inc. products are for research use only.
"Android is a trademark of Google Inc.. Qubit, Quant-iT, PicoGreen and OliGreen are trademarks of Thermo Fisher
Scientific and its subsidiaries. QuantiFluor is a trademark of Promega Corporation."

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