TECHNICAL
DATA
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Fluorescent Imaging Agent
Caution: For laboratory use only. This product is intended for animal research only and not for use in humans.

Name: GFR-Vivo 680
Product Number: NEV30000

DESCRIPTION
GFR-Vivo 680 is a near infrared fluorescent inulin based agent that, in combination with fluorescence tomography heart imaging, enables
quantitative assessment of renal Glomerular Filtration Rate, as an indicator of renal toxicity or injury.

CONTENTS Each vial contains 24 nmol of GFR-Vivo 680 in dry solid form.
GFR-Vivo 680 has been filtered through a 0.2 µm filter prior todrying.
 Reconstitute GFR-Vivo 680 with 1.2 mL of 1X PBS before
administering to animals.
 The packaged material provides sufficient reagent for imaging
approximately 10 mice (weighing ~25 grams each) when using therecommended dose of 2 nmol (in 100 μL of PBS) of GFR-Vivo 680
per mouse.

PHYSICAL AND SPECTRAL PROPERTIES
Property
MWFluorescence emission1
Absorbance1
Purity2Appearance

Specification~6,000 g mol-1
690 nm
670 nm
>90 %Blue solid

1. Absorbance and fluorescence maxima in 1x PBS.
2. As determined by RP-HPLC; measuring absorbance at 680 nm.

STORAGE & HANDLING Upon receipt, GFR-Vivo 680 should be IMMEDIATELY STORED
AT 2-8 °C AND PROTECTED FROM LIGHT.
 When stored and handled properly, GFR-Vivo 680 in its dry solid
form is stable for up to three months.
 Before opening the vial check to ensure that all of the solid material
is at the bottom of the vial.
 After reconstituting with 1X PBS, gently swirl the solution to ensurethat the solid is fully in solution.
 Once reconstituted, GFR-Vivo 680 is stable for up to 14 days
when stored at 2-8 °C and protected from light.
 Allow GFR-Vivo 680 imaging agent to equilibrate to room
temperature before introducing into animals.

Normalized absorbance (blue) and fluorescence emission (red) spectra of GFRVivo 680 in 1x PBS

IN VIVO IMAGING AND APPLICATIONS The generally recommended procedure for in vivo imaging with GFR-Vivo 680 is administration via either tail vein intravenous injection or
retro orbital injection and imaging the chest area immediately at various times points (1, 5, 15, 30, and 45 min) following injection.
 The blood half-lives of GFR-Vivo 680 in normal mice are approximately 0.5 and 8.0 min, respectively, for T1/2 Alpha and T1/2 Beta. Repeat
injection and heart imaging may be performed as early as 24 hours for longitudinal studies, although there is longer term retention in thekidneys.
 GFR-Vivo 680 enables FMT heart imaging kinetics to predict blood concentrations of inulin and to calculate GFR in SKH-1E mice.
 Please refer to the application note for step by step protocol on using this agent on an FMT.

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NOTES Caution: For laboratory use only. This product is intended for animal research only and not for use in humans. It must be used by ordirectly under the supervision of a technically qualified individual experienced in handling potentially hazardous materials. Please read theMaterial Safety Data Sheet (MSDS) provided for this product.
 Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents pending. Our products
are not available for resale or other commercial uses without a specific agreement from PerkinElmer.

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