TECHNICAL
DATA
SHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

Cat B 680 FAST™ FLUORESCENT IMAGING AGENT
Product Number: NEV11112

DESCRIPTION: Cat B 680 FAST™ is a member of a family of activatable fluorescent imaging agents comprising a novel
architecture, termed F.A.S.T. (Fluorescent Activatable Sensor Technology) that confers an improved pharmacokinetic profile
with a broader range of early imaging time points. This architecture also offers higher target specific signal with reducedbackground.
Cat B 680 FAST is a Cathepsin B activatable agent that is optically silent upon injection and produces fluorescent signal aftercleavage by Cathepsin B produced by inflammatory cells and tumor cells. Cat B 680 FAST may be used to monitor
inflammation, tumor activity, progression of disease and the efficacy of therapeutic treatment in applications such as oncology,inflammation, cardiovascular disease and some neurological diseases.

CONTENTS: Each vial contains 24 nmol of Cat B 680
FAST in dry solid form. Cat B 680 FAST has been filteredthrough a 0.2 µm filter prior to drying.
Reconstitute Cat B 680 FAST with 1.2 mL of 1 x PBS before
injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 2 nmols (100 µL) of Cat B 680 FAST per mouse. Someapplications like Atherosclerosis imaging require a higher
dose. (see note below)

PROPERTIES: The physical characteristics of Cat B
680 FAST can be found in Table 1 and Figure 1.

STORAGE & HANDLING:


1. Absorbance and fluorescence maxima of activated
Cat B 680 FAST in 1x PBS.
2. As determined by RP-HPLC and measuring absorbanceat 675 nm.

A.U.

MATERIAL (Needs to be reconstituted)

Table 1. Cat B 680 Characteristics
Property
SpecificationMW
33,000 g mol-1
Fluorescence1
 Excitation
675 nm Emission
693 nmAbsorbance
675 nm (activated)
Purity2>95 %AppearanceBlue Solid

1
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0400

500

600

700

800

900

Upon receipt, Cat B 680 FAST should be STORED
Wavelength (nm)AT 2-8 oC AND PROTECTED FROM LIGHT.
When stored and handled properly, Cat B 680 FAST
is stable for up to 3 months in dry solid form.
Figure 1. Normalized absorbance (grey), excitation (blue) and fluorescence
Before opening the vial check to ensure that all of theemission (red) spectra of Cat B 680 FAST in 1x PBS.
solid material is at the bottom of the vial.
After reconstituting with PBS, gently swirl thesolution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution isstable up to 10 days when stored at 2-8 ºC and protected from light.

Page 1 of 2

IN VIVO IMAGING AND APPLICATIONS:Cathepsin B is a cysteine protease involved in the degradation of the extracellular matrix and may be one of the key
factors in cancer progression. Cathepsin B correlates with invasiveness and metastatic capabilities in many tumors. In
breast cancer specifically, high expression levels of cathepsin B have been linked to highly aggressive tumors andpoor clinical outcome. Cathepsin B is also upregulated in a variety of inflammatory cells (including eosinophils,neutrophils, and macrophages), and may provide an inflammation readout that indicates the potential vulnerability ofAtherosclerotic plaques in Cardiovascular disease.

The generally recommended procedure for in vivo imaging with Cat B 680 FAST is administration via intravenous
injection and imaging 6-24 hours post injection. Earlier and later time points may be appropriate for some diseasemodels, and the optimal imaging time point for any application should be determined empirically. Some applications
like imaging of atherosclerotic plaques may require a 4 nmol dose and may benefit from a later imaging timepoint.

Cat B 680 FAST enables imaging of Cathepsin B activity in applications including: Cardiovascular disease, Oncology
 Inflammation
 Certain neurological diseases

NOTES:
PerkinElmer’s Cat B680 FAST is intended for research purposes only and is not for human use. It must be used byor directly under the supervision of a technically qualified individual experienced in handling potentially hazardousmaterials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement fromPerkinElmer.

PerkinElmer, Inc.
549 Albany Street
Boston, MA 02118 USA
P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc.
All other trademarks are the property of their respective owners.