TECHNICALDATASHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

OsteoSense® 680 EX
Product Number: NEV10020EX

DESCRIPTION: OsteoSense ® 680 EX is a fluorescent in vivo bisphosphonate imaging agent. OsteoSense 680 EX images
areas of microcalcifications and bone remodeling and enables imaging of bone growth and resorption.

MATERIAL: (Needs to be diluted)

CONTENTS: Each vial contains 24 nmol of OsteoSense 680 EX as a lyophilized solid. The solution has been filtered(0.2µ) prior to lyophilization. Upon dilution with 1.2 mL of 1 x PBS, this material provides sufficient reagent for imaging
approximately 10 mice (weighing ~25 grams each) when using the recommended dose of 2 nmol/100 µL 1xPBS ofOsteoSense 680 EX per mouse.
Table 1. OsteoSense 680 EX Properties
Property
Specification
MW(acidform)
1470.5g mol-1
PROPERTIES: The physical properties of OsteoSense
1Fluorescence
680 EX can be found in Table 1 and Figure 1.
 Excitation
668 ±10 nm
 Emission max
687 ±10 nm
2Absorbancemax
668 ±5 nm
STORAGE & HANDLING:
3Purity>95%
 Upon receipt, OsteoSense 680 EX should beoAppearanceBluesolid
IMMEDIATELY STORED AT 2-8 C AND
1.
AbsorbanceandfluorescencemaximaofOsteoSense
680 EX in PBS.
PROTECTED FROM LIGHT.
2. Based on concentration resulting in absorbance of 0.3 to 0.5 AU.
 When stored and handled properly, OsteoSense 680
3. As determined by RP-HPLC and measuring absorbance at 680 nm..
EX is stable for six months from date of shipment.
 Once reconstituted, the PBS solution is stable up toFig 1.
10 days when stored at 2-8°C and protected from1light.
0.8




The recommended procedure for in vivo imaging
with OsteoSense 680 EX is administration via tailvein injection and imaging 24 hours post tail vein
injection.
Imaging Bone Growth: OsteoSense 680 EX can beused to measure the effects of therapeutic stimulationof bone growth.
Imaging Bone Remodeling: OsteoSense 680 EX canbe used to characterize bone remodeling associatedwith animal models of arthritis.

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0.6

Normalized units

IN VIVO IMAGING & APPLICATIONS:

0.40.20400500600700800
Wavelength, nm900

Absorbance and fluorescence emission spectra in 1x PBS.

SELECTED REFERENCES: Zaheer, A., Lenkinski, R.E., Mahmood, A., Jones, A.G., Cantley, L.C., Frangioni, J.V. In vivo near-infrared fluorescenceimaging of osteoblastic activity. Nature Biotechnology 19, 1148-1154 (2001)

NOTES:

PerkinElmer’s OsteoSense 680 EX is intended for research purposes only and is not for human use. It must be usedby or directly under the supervision of a technically qualified individual experienced in handling potentiallyhazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S. and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement with PerkinElmer.

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