TECHNICAL
DATA
SHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

MMPSense™ 750 FAST
FLOURESCENT IMAGING AGENT
Product Number: NEV10168

DESCRIPTION: MMPSense™ 750 FAST is the first in a family of activatable fluorescent imaging agents comprising anovel architecture termed F.A.S.T. (Fluorescent Activatable Sensor Technology), that confers an improved pharmacokinetic
profile with earlier imaging time points. This architecture offers higher target specific signal with reduced background whilealso reducing the optimal imaging time after injection.
MMPSense 750 FAST is a matrix metalloproteinase (MMP) activatable agent that is optically silent upon injection andproduces fluorescent signal after cleavage by disease related MMP’s. Activation can occur by a broad range of MMP’s
including MMP 2, 3, 7, 9, 12, and 13. MMP activity is involved in many disease-related processes including cancer
progression, invasion and metastasis, rheumatoid arthritis, pulmonary diseases and areas of cardiovascular disease. MMPSense
750 FAST may be used to monitor the progression of these diseases or to evaluate the potential therapeutic efficacy of drugs
targeting the underlying mechanisms involved in these diseases.

MATERIAL: (Needs to be reconstituted)

CONTENTS: Each vial contains 24 nmol of MMPSense
750 FAST in dry solid form. MMPSense 750 FAST has been
filtered through a 0.2 µm filter prior to drying.
Reconstitute MMPSense 750 FAST with 1.2 mL of 1 x PBS
before injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 2 nmol (100 µL) of MMPSense 750 FAST per mouse.

Table 1. MMPSense 750 FAST Characteristics
Property
Specification
MW~ 43,000 g mol-1
Fluorescence1
 Excitation
749 nm Emission
775 nmAbsorbance1750 nm (activated)
Purity2>95%
Appearance
Dark blue solid
1. Absorbance and fluorescence maxima of MMPSense 750
FAST in 1x PBS.
2. As determined by RP-HPLC and measuring absorbance at
750 nm.

PROPERTIES: The physical characteristics ofMMPSense 750 FAST can be found in Table 1and Figure 1.

STORAGE & HANDLING:
Page 1 of 2Upon receipt, MMPSense 750 FAST should be
IMMEDIATELY STORED AT
2-8 oC AND PROTECTED FROM LIGHT.
When stored and handled properly, MMPSense
750 FAST is stable for up to nine months in drysolid form.
Before opening the vial check to ensure that all of
the solid material is at the bottom of the vial.

Figure 1. Normalized absorbance (grey), excitation(blue) and fluorescence emission (red) spectra ofMMPSense 750 FAST in 1x PBS.



After reconstituting with PBS, gently swirl the solution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution is stable up to 10 days when stored at 2-8 ºC and protectedfrom light.

IN VIVO IMAGING AND APPLICATIONS:The generally recommended procedure for in vivo imaging with MMPSense 750 FAST is administration via
intravenous injection and imaging 6 hours post injection.
MMPSense 750 FAST will clear from tissues after approximately 96 hours. Repeat injection and imaging may be
performed every four days for longitudinal studies. It is recommended that a pre-injection baseline image be taken
prior to reinjection and imaging.
MMPSense 750 FAST enables imaging of tumors and inflammation in a broad range of oncology, pulmonary andcardiovascular applications.

NOTES:
PerkinElmer’s MMPSense 750 FAST is intended for research purposes only and is not for human use. It must be
used by or directly under the supervision of a technically qualified individual experienced in handling potentiallyhazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S. and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement withPerkinElmer.

PerkinElmer, Inc.
549 Albany StreetBoston, MA 02118 USA
P: (800) 762-4000 or (+1) 203-925-4602
www.perkinelmer.com/nenradiochemicals
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc.
All other trademarks are the property of their respective owners.