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Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

Neutrophil Elastase 680 FAST™
Product Number: NEV11169

DESCRIPTION: Neutrophil Elastase 680 FAST™ is a member of a family of activatable fluorescent imaging agentscomprising a novel architecture, termed F.A.S.T. (Fluorescent Activatable Sensor Technology) that confers an improved
pharmacokinetic profile with a broader range of early imaging time points. This architecture also offers higher target specificsignal with reduced background.
Neutrophil Elastase 680 FAST is a neutrophil elastase activatable agent that is optically silent upon injection and produces
fluorescent signal after cleavage by elastase produced by neutrophil cells. Neutrophil elastase is a key protease involved inacute lung injury, acute respiratory distress syndrome, as well as many other inflammatory processes such as emphysema,cystic fibrosis, COPD, wound healing, rheumatoid arthritis, ischemia-reperfusion and many others. Neutrophil Elastase 680
FAST is a selective neutrophil elastase-activatable agent designed for imaging of this serine protease both in vitro and in vivo.
The half life in plasma of Neutrophil Elastase 680 FAST is 4 hours.

MATERIAL (Needs to be reconstituted)

CONTENTS: Neutrophil Elastase 680 FAST: Each vial
contains 48 nmol of Neutrophil Elastase 680 FAST in dry solidform. Neutrophil Elastase 680 FAST has been filteredthrough a 0.2 µm filter prior to drying.
Reconstitute Neutrophil Elastase 680 FAST with 1.2 mL of 1 x
PBS before injecting into animals. The packaged material
provides sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 4 nmols (100 µL) of Neutrophil Elastase 680 FAST permouse.

Table 1. Neutrophil Elastase 680 FAST Characteristics
PropertySpecification
MW~43,000 g mol-1
Fluorescence1
 Excitation
675 nm Emission
693 nmPurity2>95 %AppearanceBlue Solid
1. Absorbance and fluorescence emission maxima of activatedNeutrophil Elastase 680 FAST in 1x PBS.
2. As determined by RP-HPLC and measuring absorbanceat 675 nm.

PROPERTIES: The physical characteristics ofNeutrophil Elastase 680 FAST can be found in Table 1 andFigure 1STORAGE & HANDLING:


Upon receipt, Neutrophil Elastase 680 FAST should
be STORED AT 2-8 oC AND PROTECTEDFROM LIGHT.
When stored and handled properly, Neutrophil
Elastase 680 FAST is stable for up to 6 months in dry
solid form.
Before opening the vial check to ensure that all of thesolid material is at the bottom of the vial.

Page 1 of 2Figure 1. Absorbance (blue) and fluorescence emission (red) spectra of
Neutrophil Elastase 680 FAST in 1x PBS.



After reconstituting with PBS, gently swirl the solution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution is stable up to 7 days when stored at 2-8 ºC and protected fromlight.

IN VIVO IMAGING AND APPLICATIONS:
The generally recommended procedure for in vivo imaging with Neutrophil Elastase 680 FAST is administration via
intravenous injection and imaging 4-8 hours post injection. Earlier and later time points may be appropriate for some diseasemodels, and the optimal imaging time point for any application should be determined empirically.
Neutrophil Elastase 680 FAST enables imaging of neutrophil elastase activity in applications including: Acute lung Injury Models
 Acute respiratory distress syndrome Emphysema
 Cystic Fibrosis
 COPD Wound Healing Rheumatoid Arthritis
 Ischemia-reperfusion

NOTES:
PerkinElmer’s Neutrophil Elastase 680 FAST is intended for research purposes only and is not for human use. It
must be used by or directly under the supervision of a technically qualified individual experienced in handlingpotentially hazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

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