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Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

ReninSense 680 FAST ™
Product Number: NEV11079

DESCRIPTION: ReninSense 680 FAST™ is a member of a family of activatable fluorescent imaging agents comprisinga novel architecture, termed F.A.S.T. (Fluorescent Activatable Sensor Technology), that confers an improved pharmacokinetic
profile with a broader range of early imaging timepoints. This architecture also offers higher target specific signal with reducedbackground.
ReninSense 680 FAST is a Renin activatable agent that is optically silent upon injection and produces fluorescent signal aftercleavage by renin produced in the kidneys. The Renin-Angiotensin System (RAS) is the hormone system involved inregulating blood pressure and fluid balance in the body. ReninSense 680 FAST is recognized and activated by rodent forms ofrenin in preclinical animal models. ReninSense 680 FAST may be used to monitor abnormal RAS function, progression of
disease and the efficacy of therapeutic treatment in disorders such as hypertension and cardiovascular disease and someneurological diseases.

MATERIAL (Needs to be reconstituted)

CONTENTS: Each vial contains 24 nmol of ReninSense
680 FAST in dry solid form. ReninSense 680 FAST has been
filtered through a 0.2 µm filter prior to drying.
Reconstitute ReninSense 680 FAST with 1.2 mL of 1 x PBS
before injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 2 nmol (100 µL) of ReninSense 680 FAST per mouse.

Table 1. ReninSense 680 FAST Characteristics
Property
SpecificationMW
43,000 g mol-1
1Fluorescence Excitation
675 nm Emission
693 nmAbsorbance
675 nm (activated)
Purity2>95 %Appearance
Dark Blue Solid
1. Absorbance and fluorescence maxima ofReninSense 680 FAST in 1x PBS.
2. As determined by RP-HPLC and measuring absorbanceat 675 nm.

PROPERTIES: The physical characteristics ofReninSense 680 FAST can be found in Table 1 andFigure 1.

STORAGE & HANDLING:


Upon receipt, ReninSense 680 FAST should be
STORED AT -20 oC AND PROTECTED FROM
LIGHT.
When stored and handled properly, ReninSense 680
FAST is stable for up to twelve months in dry solidform.
Before opening the vial check to ensure that all of thesolid material is at the bottom of the vial.

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Figure 1. Normalized absorbance (grey), excitation (blue)and fluorescence emission (red) spectra of ReninSense 680
FAST in 1x PBS.



After reconstituting with PBS, gently swirl the solution to ensure that the solid is fully in solution.
Once reconstituted with 1 x PBS, the solution is stable up to 7 days when stored at 2-8 ºC and protected fromlight.

IN VIVO IMAGING AND APPLICATIONS:Renin is a peptide hormone secreted by the kidney from granular cells of the juxtaglomerular apparatus and is the firststep in a cascade of events that lead to increased blood pressure. Renin is upregulated in response to decrease inarterial blood pressure, decrease in plasma sodium chloride levels, or sympathetic nervous system activity. Renin
cleaves angiotensinogen (produced in the liver) to yield angiotensin I, which is further converted to angiotensin II byangiotensin-converting enzyme (ACE). Angiotensin II constricts blood vessels, increases aldosterone secretion fromthe adrenals, and stimulates a thirst reflex, each leading to an increase in blood pressure. Kidney imaging ofReninSense 680 FAST can detect 3- to 4-fold increases in kidney renin activity in response to 2-3 days of low salt dietin C57BL/6 mice.

The generally recommended procedure for in vivo imaging with ReninSense 680 FAST is administration via
intravenous injection and imaging 24 hours post injection. Earlier and later timepoints may be appropriate for somedisease models, and the optimal imaging timepoint for any application should be determined empirically.

ReninSense 680 FAST enables imaging of Renin activity in applications including:cardiovascular disease, certain models of impaired renal function,
 chronic hyperthyroidism,
 hypertension, and some neurological diseases.

NOTES:
PerkinElmer’s ReninSense 680 FAST is intended for research purposes only and is not for human use. It must beused by or directly under the supervision of a technically qualified individual experienced in handling potentiallyhazardous materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

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