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Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

Cat K™ 680 FAST
Product Number: NEV11000

DESCRIPTION: Cat K™ 680 FAST is a member of a family of activatable fluorescent imaging agents comprising anovel architecture, termed F.A.S.T. (Fluorescent Activatable Sensor Technology), that confers an improved pharmacokinetic
profile with earlier imaging time points. This architecture offers higher target specific signal with reduced background while
also reducing the optimal imaging time after injection.
Cat K 680 FAST is a Cathepsin K (Cat K) activatable agent that is optically silent upon injection and produces fluorescentsignal after cleavage by disease related Cathepsin K. Cat K is highly expressed in osteoclasts, suggesting a specialized role inbone resorption. Cat K is found extracellularly along the bone and cartilage and intracellularly in lysozomes of osteoclasts
attached to the bone. In addition to expression by osteoclasts, Cat K is also expressed by a broad range of other cell types
including ovarian cells, colonic tissue, alveolar and bronchial epithelial cells, synovial fibroblasts, breast cancer cells,macrophages.

MATERIAL (Needs to be reconstituted)

CONTENTS: Each vial contains 24 nmol of Cat K 680
FAST in dry solid form. Cat K 680 FAST has been filteredthrough a 0.2 µm filter prior to drying.
Reconstitute Cat K 680 FAST with 1.2 mL of 1 x PBS before
injecting into animals. The packaged material provides
sufficient reagent for imaging approximately 10 mice
(weighing ~25 grams each) when using the recommended doseof 2 nmol (100 µL) of Cat K 680 FAST per mouse.

Table 1. Cat K 680 FAST Characteristics
PropertySpecification
MW8500 g mol-1
1Fluorescence Excitation
675 nm Emission
693 nmAbsorbance
675 nm (activated)
Purity2>95 %Appearance
Dark Blue solid
1. Absorbance and fluorescence maxima of
Cat K 680 FAST in 1x PBS.
2. As determined by RP-HPLC and measuring absorbanceat 675 nm.

PROPERTIES: The physical characteristics of
Cat K 680 FAST can be found in Table 1 and Figure
1

STORAGE & HANDLING:


Upon receipt, Cat K 680 FAST should be
IMMEDIATELY STORED AT 2-8 oC ANDPROTECTED FROM LIGHT.
When stored and handled properly, Cat K 680 FAST
is stable for up to 1 year in dry solid form.
Before opening the vial check to ensure that all of thesolid material is at the bottom of the vial.
After reconstituting with PBS, gently swirl thesolution to ensure that the solid is fully in solution.

Page 1 of 2Figure 1. Normalized absorbance (grey), excitation (blue)and fluorescence emission (red) spectra of Cat K 680 FAST in
1x PBS.

Once reconstituted with 1 x PBS, the solution is stable up to 10 days when stored at 2-8 ºC and protected fromlight.

IN VIVO IMAGING AND APPLICATIONS:The generally recommended procedure for in vivo imaging with Cat K 680 FAST is administration via intravenous
injection and imaging 6 hours post injection.
Cat K 680 FAST enables imaging of Cathepsin K activity in Oncology applications involving metastasis to the bone
as well as a broad range of bone applications including osteoporosis and bone changes following arthritis

NOTES:
PerkinElmer’s Cat K 680 FAST is intended for research purposes only and is not for human use. It must be used byor directly under the supervision of a technically qualified individual experienced in handling potentially hazardous
materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.

Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

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