TECHNICAL
DATA
SHEET

Fluorescent Imaging Agent
Caution: For Laboratory Use. A product for research purposes only.

GastroSense™ 750
Product Number: NEV11121

DESCRIPTION: GastroSense™ 750 is a near-infrared, fluorescently-labeled macromolecule that may be used to monitor
the effects of disease or drugs on gastric motility and or gastric emptying. It may also be used as an anatomical marker for thegastrointestinal tract.

MATERIAL (Needs to be reconstituted)

CONTENTS: Each vial contains 3 nmol of GastroSense
750 in dry solid form. Reconstitute GastroSense 750 with 1.2mL of 1 x PBS before gavaging or feeding to animals. The
packaged material provides sufficient reagent for imaging
approximately 10 mice (weighing ~25 grams each) when using
the recommended dose of 0.25 nmol (in 100 μL of PBS) ofGastroSense 750 per mouse.

PROPERTIES: The physical properties of GastroSense

Table 1. GastroSense 750 Properties
Property
Specification
MW~40,000 g mol-1
1Fluorescence Excitation
750 ± 5 nm
 Emission
770 ±5 nm
Absorbance
750 ±5 nm
Purity2>95 %Appearance
Clear blue solution
1.
2.

Absorbance and fluorescence maxima of GastroSense 750 in 1x PBS.
As determined by SE-HPLC and measuring absorbance at 750 nm.

Fig 1.

750 can be found in Table 1 and Figure 1.

STORAGE & HANDLING:


Upon receipt, GastroSense 750 should be
IMMEDIATELY STORED AT 2-8 oC ANDPROTECTED FROM LIGHT.
When stored and handled properly, GastroSense 750
in its dry solid form is stable for up to six months.
Once reconstituted, GastroSense 750 is stable forup to 10 days when stored at 2-8 0C and protectedfrom light.
Allow GastroSense 750 imaging agent to equilibrateto room temperature before introducing into animals.

Absorbance and fluorescence emission spectra in 1x PBS.

IN VIVO IMAGING AND APPLICATIONS:GastroSense may be reconstituted with 1x PBS. A methylcellulose solution may also be used or it may bereconstituted with a liquid diet supplement solution.
The recommended procedure for in vivo imaging with GastroSense 750 is administration via gavage or in solid food(egg yolk) and then imaging at the desired time points beginning immediately after ingestion.

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GastroSense may also be administered as part of a solid diet by applying the reconstituted solution to egg yolk. Whenadded to a solid diet it may be easier to reconstitute the vial of GastroSense 750 with 300 µL of 1x PBS and use 25µL per mouse.
Clearance times will depend on the method of administration and the animal model used. For example when gavaged
as a liquid in normal healthy mice the average half-life in the stomach may be as little as 15-30 minutes though times
will vary significantly based on age, sex and strain of animal. When GastroSense 750 is administered as part of a solid
diet clearance half-lives typically increase.
GastroSense remains localized in the GI tract. It does not appear to be absorbed into the bloodstream.

APPLICATIONS:Imaging in Gastric Emptying: GastroSense 750 may be used to study the effect of drugs or disease progression on
gastric emptying or gastric motility.
Imaging as an anatomical marker: GastroSense 750 may be used as an anatomical reference marker for imaging thegastrointestinal tract.

NOTES:
PerkinElmer’s GastroSense750 is intended for research purposes only and is not for human use. It must be used byor directly under the supervision of a technically qualified individual experienced in handling potentially hazardous
materials. Please read the Material Safety Data Sheet (MSDS) provided for this product.



Several of PerkinElmer’s products and product applications are covered by U.S and foreign patents and patents
pending. Our products are not available for resale or other commercial uses without a specific agreement from
PerkinElmer.

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