Instructions For Use

LBC-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4

Page 1 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Luxol Fast Blue Stain Kit
Description:

The Luxol Fast Blue Stain Kit is designed for staining myelin/myelinated axons and Nissil substance on
formalin fixed, paraffin-embedded tissue. This product is used for identifying the basic neuronal
structure in brain or spinal cord sections.
Myelinated Fibers:
Nissil Substance:Nerve Cells:

BlueViolet
VioletUses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

Cerebral Cortex
Spinal Cord

Ordering information regarding individual components
on back page!
Kit Contents:
Item #CEA125
LFB125
LCQ500EAS500Precautions:

Kit Contents
Cresyl Echt Violet Solution
Luxol Fast Blue Solution
Lithium Carbonate Solution (0.05%)
Alcohol, Reagent (70%)

Storage
2-8° C
18-25°C
18-25°C
18-25°C

Avoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

Volume125 ml
125 ml500 ml500 ml

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CV

P
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For Use

LBC-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4Page 2 of 2P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Procedure:
1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Incubate slide in Luxol Fast Blue Solution for 24 hours at room temperature or 2 hours at 60°C.

3.

Rinse thoroughly in distilled water.

4.

Differentiate section by dipping in Lithium Carbonate Solution (0.05%) several times (up to 20 seconds).

5.

If needed, continue differentiation by repeatedly dipping in Alcohol, Reagent (70%) until gray-matter iscolorless and white-matter remains blue.

6.

Rinse slide in 2 changes of distilled water.

7.

Incubate slide in Cresyl Echt Violet (0.1%) for 2-5 minutes.

8.

Rinse quickly in 1 change of distilled water.

9.

Dehydrate quickly in 3 changes of absolute alcohol.

10.

Clear as desired and mount in synthetic resin.

References:1.

Sheenan, D.C., Hrapchak, B.B. Theory and Practice of Histotechnology, 2nd Edition. Battelle Press, Columbus, OH. Page 262-264. 1980

2.

Kluver, H., Barrera, E.A. A Method for the combined staining of cells and fibers in the nervous system. Journal of Neuropathology andExperimental Neurology, 1953, 12: pages 400-403.

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Cresyl Echt Violet Solution

CEA125
CEA500
CEA999125 ml500 ml1000 ml

Luxol Fast Blue Solution

LFB125
LFB500
LFB999125 ml500 ml1000 ml

Lithium Carbonate Solution (0.05%)

LCQ500
LCQ999500 ml
1000 ml

Alcohol, Reagent (70%)

EAS500
EAS999500 ml
1000 ml

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands