Surveeyor® Mutatio
Mon Detection Kit for W
WAVE® aand WA
AVE HS System
msDiscoveer and Dete
ect All Typess of Genetic Variations
Surveyor Mutationn Detectionn Kits provid
de a simple,accuratte and cost‐‐effective means to scan DNA fragm
ments
for muttations withh unmatchedd sensitivityy and specifiicity.
Compleete Mutatioon Detectionn System: Contains
CSurrveyor
Nucleasse, all reageents and plaasmid controols.
Surveyoor Nuclease isa mismaatch‐specificendonucclease thatrecognizzes mismatches
in heterooduplexed DN
NAand cleaaves bothstrands on the 3’ sideemismatchof the mprovidin
ng specificinformattion onmutation location,orientattion and type.

Figuure 1. Detection of sequence varriant in mitochon
ndrial DNA using
gSurvveyor Nuclease aand the fragmen
nt analysis function of the WAVE
E HS
Systtem.

Designeed forMultiplle Mutationn Discovery Application
nsSommatic, germ‐‐line and mittochondrial mutations
Inseertions, deleetions, substtitutions andd SNPs
Low
w‐copy mutaations and pooledpDNA samplesin Four EaMutation Detection
Dasy Steps
Ampliffy Mutant and Reference DNAHybridize DN
NA Hetero- and Homoduplex Formation

AddA
SURVEYO
OR Nuclease

Figuure 2. WAVE HS aanalysis of Surveeyor Nuclease trreated TSC1 exon 20
PCR product from pprostate tumor. AAnalysis indicatees that a low
minoority mutation iss present in the sample, and thee mutation isapprroximately 50 bpp from one end of the 220‐bp PCR product.

Benefitts
Dettermines muutation loci
Saves time andd money– Streamlines DNA sequencing– Decrease
es sequencing backlog and analysis time
– Validates sequencinng results annd analysis– Decrease
es chromato
ogram analyysis by 90%
pNo post‐PCR orr post‐reaction clean‐uph throughpu
ut sensitivity and specifficity
HighFiguure 3. Surveyor ddetection of patient with hetero
ozygous germ‐line
muttation in the 7266‐bp amplicon sp
panning the coree promoter regio
on of
the PTEN gene – Cowden Syndromee.

Surveyor Mutation Detection Kit Components – All Optimized to Work Together• Surveyor Nuclease W• Surveyor Nuclease Enhancer W2• Surveyor Nuclease Enhancer Cofactor•Stop Solution•Control Plasmids and Primers• 0.15M Mg2Cl SolutionProducts

Surveyor Mutation Detection Kit for WAVE and WAVE HS Systems
Quantity
25 reactions
100 reactions
1,000 reactions

Catalog No.
706035706030706031

Shipping and Storage
Surveyor products are shipped frozen. Store product at ‐20 ºC in a non‐frost‐free freezer. Enzyme is guaranteed for aperiod of 6 months if stored as directed.

Quality Control
Every component is quality control tested as indicated on the certificate of analysis, included in each kit.

Selected References
1. DHPLC/Surveyor Nuclease: A Sensitive, Rapid and Affordable Method to Analyze BRCA1 and BRCA2 Mutations in Breast Cancer Families. Pilato, B.
et al. Mol. Biotechnol. 52, 8‐15. (2011).
2. A molecular analysis of mutations at the complex dumpy locus in Drosophila melanogaster. Carmon, A. et al. PLoS ONE 5, e12319. (2010).
3. Screening for mutations in kidney‐related genes using Surveyor nuclease for cleavage at heteroduplex mismatches. Voskarides, K. & Deltas, C. J. Mol.
Diagn. 11, 311‐318. (2009).
4. Novel method for genomic analysis of PKD1 and PKD2 mutations in autosomal dominant polycystic kidney disease. Tan, Y. et al. Hum. Mutat. 30,
264‐272. (2009).
5. Single nucleotide polymorphisms, apoptosis, and the development of severe late adverse effects after radiotherapy. Azria, D. et al. Clin. Cancer Res. 14,6284‐6288. (2008).
6. Rapid identification of unknown heteroplasmic mutations across the entire human mitochondrial genome with mismatch‐specific Surveyor Nuclease
Bannwarth, S. et al. Nat. Protoc. 1, 2037‐2047. (2006).
7. Mitochondrial DNA damage and repair in RPE associated with aging and age‐related macular degeneration. Lin, H. et al. Invest. Ophthalmol. Vis. Sci.
52, 3521‐3529. (2011).
8. Allelic dilution obscures detection of a biologically significant resistance mutation in EGFR ‐amplified lung cancer. Engelman, J.A. et al. J. Clin. Invest.
116, 2695‐2706. (2006).
9. A rapid and sensitive enzymatic method for epidermal growth factor receptor mutation screening. Jänne, P.A. et al. Clin. Cancer Res. 12, 751‐758.
(2006).
10. Temperature‐mediated heteroduplex analysis for the detection of drug‐resistant gene mutations in clinical isolates of Mycobacterium tuberculosis bydenaturing HPLC, Surveyor nuclease. Shi, R. et al. Microbes Infect. 8, 128‐135. (2006).

www.IDTDNA.com/Surveyor
For questions or additional assistance, please contact IDT Customer Care at:
USA & Canada
custcare@idtdna.com
1–800–328–2661

Europeeutechsupport@idtdna.com+32 (0) 16 28 22 60

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1800 092 259

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owners. For specific trademark and licensing information, see www.idtdna.com/trademarks.
Surveyor products are covered by multiple IDT patents and are sold by IDT under exclusive license to U.S. Patents 6,391,557, 5,869,245. This product is sold for non‐commercial, research use only. Any non‐clinical, commercial use, including without limitation the use of any Surveyor product in the manufacture of nucleic acidproducts of any type, requires a separate license from IDT. Any clinical use of any Surveyor product requires a separate license from Transgenomic, the exclusive licenseeof Surveyor products in the clinical and diagnostic fields. Surveyor is a registered trademark of IDT.