Instructions For Use

BSK-1-IFU
Rev. Date: 7/9/2018

Revision: 3

Page 1 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Bielschowsky’s Stain Kit (Modified)
Description:

The Bielschowsky’s Stain Kit (Modified) is designed for histological visualization of nerve fibers,
neurofibrillary tangles and senile plaques in Alzheimer’s disease.
Axons:
Neurofibrillary Tangles:
Senile Plaques:
Nuclei:Background:BlackBlackBlack
Dark Brown
Yellow to Light Brown

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

Cerebral cortex (cut 8-10µm)

Ordering Information and Current Pricing at www.scytek.com

Availability:
Item #Kit Contents

VolumeStorage

SNZ500
FRL008CAS008NAS008
STB125

Silver Nitrate Solution (20%)
Formalin Solution (20%)
Citric Acid Solution (Bielschowsky’s)
Nitric Acid Solution (Bielschowsky’s)
Sodium Thiosulfate Solution (5%)

500 ml8 ml Dropper
8 ml Dropper
8 ml Dropper
125 ml

2-8°C18-25°C
18-25°C
18-25°C
18-25°C

Required but Not Included:

Concentrated Ammonium hydroxide (25-30%).

Storage:

Mixed storage conditions. Store according to individual label instructions.

Precautions:

Avoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.
25° C

Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands

Instructions For UseBSK-1-IFU
Rev. Date: 7/9/2018

Revision: 3

Page 2 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Preparation of Reagents Prior to Beginning:
1.

Prepare working Ammoniacal Silver Solution (used in step 4) using chemically cleaned glassware in a chemical fume
hood as follows:
Pour 25-50ml of Silver Nitrate Solution (20%) into container (volume used is dependent on amount required to adequately
fill staining container). Add concentrated ammonium hydroxide (25-30%) (not included in kit); drop by drop, while swirlingthe flask continuously, until precipitate just dissolves and the reagent goes clear. NOTE: If a small excess of ammoniumhydroxide (25-30%) is added and solution will not go completely clear, filter the solution using a paper filter prior to use!
Note: Use extreme care in preparation and use of Ammoniacal Silver Solution. Use mixture once and dispose.
Dispose of waste observing all local, state and federal laws.

2.

Prepare Developer Solution (used in step 8) using chemically cleaned glassware immediately prior to use as follows:
Swirl carefully throughout mixing steps.
50 ml
Distilled Water
8 Drops Formalin Solution (20%)
8 Drops Citric Acid Solution (Bielschowsky’s)
4 Drops Nitric Acid Solution (Bielschowsky’s)

3.

Prepare working Ammonia Water (used in step 8) by mixing 320µl (8 drops) of concentrated Ammonium hydroxide (2530%) (not included) in 50 ml of distilled water.

Procedure:
Preheat waterbath to 40°C
1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Place a chemically cleaned staining jar containing 25ml of Silver Nitrate Solution (20%) in waterbath and allow temperature
to equilibrate for 10 minutes.

3.

Place slide in warmed Silver Nitrate Solution (20%) and incubate for 15 minutes at 40°C.

4.

During incubation place Ammoniacal Silver Solution in waterbath in allow temperature to equilibrate.

5.

Remove slide from Silver Nitrate Solution (20%) and rinse in 4 changes of distilled water.

6.

Place slide in warmed Ammoniacal Silver Solution and incubate for 10 minutes at 40°C.

7.

Remove slide from Ammoniacal Silver Solution, shake off excess and place directly into Developer Solution. Agitate gently
until tissue section takes on a yellow/brown hue (5-20 seconds).

8.

Remove slide from Developer Solution and immediately place in Ammonia Water for 30 seconds.

9.

Rinse in 4 changes of distilled water.

10.

Apply adequate Sodium Thiosulfate Solution (5%) to completely cover tissue section and incubate for 2 minutes.

11.

Rinse in 4 changes of distilled water.

12.

Dehydrate in 3 changes of absolute alcohol for 2 minutes each.

13.

Clear, and mount in synthetic resin.
25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands

Instructions For Use

BSK-1-IFU
Rev. Date: 7/9/2018

Revision: 3

Page 3 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CV
P EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands