Instructions For UseTRM-IFU

Rev. Date: Feb. 22nd, 2022

Revision: 8

Page 1 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Trichrome Stain Kit (Modified Masson’s)
Description:

The Trichrome Stain Kit (Modified Masson’s) is intended for use in the histological visualization ofcollagenous connective tissue fibers in tissue sections. This kit may be used on formalin-fixed, paraffinembedded sections.
Tissue Component:
Collagen:Muscle Fibers:
Nuclei:

Uses/Limitations:

Positive
Control Tissue:

Staining Color/Pattern:
BlueRedDark Red to Black/Blue

Not to be taken internally.
For In-Vitro Diagnostic use.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.
Tissueue
Lung
Liver
Colon
StomachOrdering information regarding individual components
on back page!
Kit Contents:
Item #BNF125
HWI-A-125
HWI-B-125
BSU125
PPA125
ABP125
AAE125

Precautions:

Storage: 18° C

Components
Bouin’s Fluid
Weigert’s Iron, Hematoxylin (A)Weigert’s Iron, Hematoxylin (B)Biebrich Scarlet / Acid Fuchsin Solution-1 Kit
125 ml125 ml125 ml125 mlPhosphomolybdic/Phosphotungstic Acid Solution 125 ml
Aniline Blue Solution
125 mlAcetic Acid Solution (1%)
125 ml

-2 Kit-500 Kit
2 x 30 ml 500 ml
30 ml
500 ml30 ml
500 ml30 ml
500 ml30 ml
500 ml30 ml
500 ml30 ml
500 mlAvoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

25° C

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
435-755-9848
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Storage18-25°C
18-25°C
18-25°C
18-25°C
18-25°C
18-25°C
18-25°C

Instructions For UseTRM-IFU

Rev. Date: Feb. 22nd, 2022

Revision: 8Page 2 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Procedure:
1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Preheat Bouin’s Fluid in a water bath to 56° - 64° centigrade in a fume hood or very well-ventilated area.

3.

Place slides in preheated Bouin’s Fluid for 60 minutes followed by a 10-minute cooling period.

4.

Rinse slide in tap water until section is completely clear.

5.

Rinse once in distilled water.

6.

Mix equal parts of Weigert’s (A) and Weigert’s (B) and stain slide with working Weigert’s Iron Hematoxylin for 2-10minutes. Stain is alcoholic and prone to evaporation – monitor and add stain as necessary to ensure stain does not dry onslide. Dried stain may result in excess grey background.

7.

Rinse slide in running tap water for 2 minutes.

8.

Rinse slide in distilled water.

9.

Apply Biebrich Scarlet / Acid Fuchsin Solution to slide for 5-10 minutes.

10.

Rinse slide in distilled water.

11.

Differentiate in Phosphomolybdic/Phosphotungstic Acid Solution for 10-15 minutes.

12.

Without rinsing, apply Aniline Blue Solution to slide for 5-10 minutes.

13.

Rinse slide in distilled water.

14.

Apply Acetic Acid Solution (1%) to slide for 3-5 minutes.

15.

Dehydrate very quickly in 2 changes of 95% Alcohol, followed by 2 changes of Absolute Alcohol.

16.

Clear in Xylene or Xylene Substitute, and mount in synthetic resin.

Notes: If final background is a dark or dull red/blue, it may be caused by excess grey background from the Weigert’s IronHematoxylin. If this is the case, reduce staining time of Working Weigert’s Iron Hematoxylin for future slides (step 6). A standard
Acid Alcohol solution (not provided) may also be used to remove excess grey background immediately after step 6 as well.
References:
1.
2.
3.
4.

Sheehan, DC., Hrapchak, BB. Theory and Practice of Histotechnology; 1980, page 190.
A.F.I.P. Laboratory Methods in Histotechnology; 1992, pages 132-133.
Bekeredjian R., Walton C.B., MacCannell K.A., Ecker J., Kruse F., et al. Conditional HIF-1α Expression Produces a Reversible
Cardiomyopathy. PLoS ONE 5(7): e11693. Doi:10.1371/journal.pone.0011693. (2010)
Zwaans BM, Krueger S, Bartolone SN, Chancellor MB, Marples B, Lamb LE. Modeling of chronic radiation-induced cystitis in mice. Advancesin Radiation Oncology. 2016 Aug 1.

Storage: 18° C

25° C

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
435-755-9848
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For UseTRM-IFU

Rev. Date: Feb. 22nd, 2022

Revision: 8Page 3 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Bouin’s Fluid

BNF125
BNF500
BNF999125 ml500 ml1000 ml

Hematoxylin, Weigert’s Iron Kit(Two Components)

HWI-1
HWI-2
HWI-3

250 ml1000 ml2000 ml

Biebrich Scarlet / Acid Fuchsin
Solution

BSU125
BSU500
BSU999125 ml500 ml1000 ml

Phosphomolybdic/Phosphotungstic
Acid Solution

PPA125
PPA500
PPA999125 ml500 ml1000 ml

Aniline Blue Solution

ABP125
ABP500
ABP999125 ml500 ml1000 ml

Acetic Acid Solution (1%)

AAE125
AAE500
AAE999125ml
500 ml1000 ml

Storage: 18° C

25° C

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
435-755-9848
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands