Instructions For Use

APS-1-IFU
Rev. Date: April 24, 2013

Revision: 5

Page 1 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Alcian Blue – PAS Stain Kit
Description:

The Alcian Blue - PAS Stain Kit is intended for use in the simultaneous histological visualization of
sulfated and carboxylated acid mucopolysaccharides, sulfated and carboxylated sialomucins(glycoproteins) and neutral mucins.
Acidic Sulfated Mucosubstances:
Hyaluronic Acid:
Sialomucins:
Neutral Mucins:
Mixtures of Acidic and Neutral Mucins:

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

Small or large Intestine
AppendixColonBlueBlueBlue
MagentaBlue - Mauve depending on dominant entity.

Ordering information regarding individual components
on back page!

Kit Contents:
Item #AAG125
ANC250
PAQ250
SRF250
HMM125

Precautions:

Kit Contents
Acetic Acid Solution (3%)
Alcian Blue Solution, pH 2.5
Periodic Acid Solution (1%)
Schiff’s Solution
Hematoxylin, Mayer’s (Lillie’s)

Volume125 ml x 2
250 ml
250 ml
250 ml125 ml x 2

Avoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

Storage18-25°C
18-25°C
2-8°C
2-8°C
18-25°C

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands

Instructions For UseAPS-1-IFU
Rev. Date: April 24, 2013

Revision: 5

Page 2 of 2P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Procedure (Room Temperature):
1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Apply Acetic Acid Solution (3%) to tissue section for 2 minutes.

3.

Remove excess Acetic Acid Solution (3%) and without rinsing apply Alcian Blue (pH 2.5) solution for 15-20 minutes.

4.

Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.

5.

Apply Periodic Acid Solution to tissue section for 5 minutes.

6.

Rinse slide in 2 changes of distilled water.

7.

Apply Schiff’s Solution to tissue section for 10-20 minutes.

8.

Rinse for 2 minutes in warm running tap water followed by 2 changes of distilled water.

9.

Apply Hematoxylin, Mayer’s (Lillie’s Modification) to tissue section for 2 minutes.

10.

Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.

11.

Dehydrate through graded alcohols.

12.

Clear, and mount in synthetic resin.

References:
1.
2.
3.
4.

Lillie, R.D. 1977, H.J. Conn’s Biological Stains, 9th Edition. Williams & Wilkins, Baltimore. Pages 452-455.
Sheenan, D.C., Hrapchak, B.B. Theory and Practice of Histotechnology, 2nd Edition. Battelle Press, Columbus, OH. Pages 172-173.
Churukian, C.J., 1989, Manual of Special Stains Laboratory, 4th Edition. University of Rochester, Rochester, New York. Pages 55-56.
Carson, F.L., 1996, Histotechnology; A Self-Instructional Text, 2nd Edition. ASCP Press, Chicago, IL. Pages 117-121.

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Acetic Acid Solution (3%)

AAG500
AAG999500 ml1000 ml

Alcian Blue Solution, pH 2.5

ANC250ANC500
ANC999ANC3800

250 ml500 ml1000 ml
1 Gallon

Periodic Acid Solution (1%)

PAQ250
PAQ500
PAQ999250 ml500 ml1000 ml

Schiff’s Solution

SRF250
SRF500
SRF999250 ml500 ml1000 ml

Hematoxylin, Mayer’s(Lillie’s Modification)

HMM500
HMM999
HMM3800

500 ml1000 ml
1 Gallon

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands