MATERIAL SAFETY DATA SHEETTransgenomic, Inc.
Effective Date: June 13, 2012
12325 Emmet Street
Document No.: MSD-70603X
Omaha, NE 68164
Revision No: 1
Emergency Phone (USA): 800-424-9300 (CHEMTREC 24hr)
Emergency Phone (UK/Europe): +44 (0)-870-190-6777 (CARECHEM24)

SECTION 1. Product Identification
Catalogue Number: 706030, 706031, 706035
Name: SURVEYOR® Mutation Detection Kit for WAVE & WAVE-HS

SECTION 2. Composition/Information on Ingredients
Substance Name: SURVEYOR® Mutation Detection Kit for WAVE & WAVE-HS
CAS#: None
SARA 313: No
The hazards identified with this kit are those associated with the following
components.
706053V, 706064V, 706065V, 706066V, 708025, 708030, 708033, 708042,708048, 708053, 708060, 708061
SECTION 3. Hazards Identification
Emergency Overview
CAUTION! MAY CAUSE IRRITATION TO SKIN, EYES, ANDRESPIRATORY TRACT. MAY AFFECT KIDNEYS. HARMFUL IF
SWALLOWED OR INHALED.
Potential Health Effects
Inhalation:
Due to the low vapor pressure, inhalation of the vapors at room temperaturesis unlikely. Inhalation of mist may cause irritation of respiratory tract.
Symptoms may include coughing, shortness of breath.
Ingestion:
Mild alkali. Causes irritation and reddening to the mucous membranes of themouth, esophagus, and gastrointestinal tract. Symptoms may include nausea,vomiting and diarrhea. Large oral doses may cause weakness, collapse, bloodclotting and coma. Estimated lethal dose: 50 grams of pure TRIS solid.

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Skin Contact:
May cause irritation.
Eye Contact:
May cause irritation.
Chronic Exposure:
May cause kidney injury. Chronic dermatitis may follow skin contact.
Aggravation of Pre-existing Conditions:
Persons with pre-existing skin disorders or eye problems or impaired liver orkidney function may be more susceptible to the effects of the substance.

SECTION 4. First Aid Measures
Inhalation:
Remove to fresh air. Get medical attention for any breathing difficulty.
Ingestion:
Induce vomiting immediately as directed by medical personnel. Nevergive anything by mouth to an unconscious person. Get medicalattention.
Skin Contact:
Immediately flush skin with plenty of water for at least 15 minutes.
Remove contaminated clothing and shoes. Wash clothing before reuse.
Thoroughly clean shoes before reuse. Get medical attention if irritation
develops.
Eye Contact:
Immediately flush eyes with plenty of water for at least 15 minutes,
lifting upper and lower eyelids occasionally. Get medical attention ifirritation persists.

SECTION 5. Fire Fighting Measures
Fire:
Flash point: 199C (390F) CC
Autoignition temperature: 370C (698F)
Slight fire hazard when exposed to heat or flame.
Explosion:
Above flash point, vapor-air mixtures are explosive within flammablelimits noted above.
Fire Extinguishing Media:
Use any means suitable for extinguishing surrounding fire. Water
spray, dry chemical, alcohol foam, or carbon dioxide extinguishers areeffective. Water spray may be used to extinguish surrounding fire andcool exposed containers. Water spray will also reduce fume and irritantgases.
Special Information:
In the event of a fire, wear full protective clothing and NIOSHapproved self-contained breathing apparatus with full facepiece
operated in the pressure demand or other positive pressure mode.

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SECTION 6. Accidental Release Measures
Ventilate area of leak or spill. Wear appropriate personal protective
equipment as specified in Section 8. Contain and recover liquid whenpossible. Collect liquid in an appropriate container or absorb with aninert material (e. g., vermiculite, dry sand, earth), and place in achemical waste container. Do not use combustible materials, such as
saw dust. Do not flush to sewer!

SECTION 7. Handling and Storage
Keep in a tightly closed container, stored in a cool, dry, ventilated area.
Protect against physical damage. Isolate from incompatible substances.
Containers of this material may be hazardous when empty since theyretain product residues (vapors, liquid); observe all warnings andprecautions listed for the product.

SECTION 8. Exposure Controls/Personal Protection
Airborne Exposure Limits:
For Glycerol Mist:- OSHA Permissible Exposure Limit (PEL):
Total Dust: 15 mg/m3 (TWA);
Respirable Fraction: 5 mg/m3(TWA).
- ACGIH Threshold Limit Value (TLV):
10 mg/m3
Ventilation System:
A system of local and/or general exhaust is recommended to keep
employee exposures below the Airborne Exposure Limits. Local
exhaust ventilation is generally preferred because it can control theemissions of the contaminant at its source, preventing dispersion of it
into the general work area. Please refer to the ACGIH document,
Industrial Ventilation, A Manual of Recommended Practices, mostrecent edition, for details.
Personal Respirators (NIOSH Approved):
If the exposure limit is exceeded and engineering controls are notfeasible, a half face piece particulate respirator (NIOSH type P95 orR95 filters) may be worn for up to ten times the exposure limit or themaximum use concentration specified by the appropriate regulatory
agency or respirator supplier, whichever is lowest. A full-face piece
particulate respirator (NIOSH type P100 or R100 filters) may be wornup to 50 times the exposure limit, or the maximum use concentration
specified by the appropriate regulatory agency, or respirator supplier,whichever is lowest. Please note that N filters are not recommended forthis material. For emergencies or instances where the exposure levels
are not known, use a full-face piece positive-pressure, air-supplied

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respirator. WARNING: Air-purifying respirators do not protect
workers in oxygen-deficient atmospheres.
Skin Protection:
Wear protective gloves and clean body-covering clothing.
Eye Protection:
Use chemical safety goggles. Maintain eye wash fountain and quickdrench facilities in work area.

SECTION 9. Physical and Chemical Properties
Appearance:
Clear oily liquid.
Odor:
Odorless.
Solubility:
Miscible in water.
Specific Gravity:
No Information FoundpH:
No Information Found% Volatiles by volume @ 21C (70F):
0Boiling Point:
219C (426F)
Melting Point:
18C (64F)
Vapor Density (Air=1):
No Information Found
Vapor Pressure (mm Hg):
No Information Found
Evaporation Rate (BuAc=1):
No information found.

SECTION 10. Stability and Reactivity
Stability:
Stable under ordinary conditions of use and storage.
Hazardous Decomposition Products:
Toxic gases and vapors may be released if involved in a fire. Glycerol
decomposes upon heating above 290C, forming corrosive gas(acrolein).
Hazardous Polymerization:Will not occur.
Incompatibilities:
Strong oxidizers. Can react violently with acetic anhydride, calcium
oxychloride, chromium oxides and alkali metal hydrides. Copper,brass, and aluminium.

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Conditions to Avoid:Heat, flames, ignition sources and incompatibles.

SECTION 11. Toxicological Information
Glycerol: oral rat LD50: 12,600 mg/kg. Investigated as a mutagen,reproductive effector.
Tris: oral rat LD50: 5900 mg/kg.
Cancer Lists
Ingredient
Glycerol (56-81-5)
TRIS (77-86-1)

Known
NoNo

NTP Carcinogen
Anticipated IARC Category
No
None
No
None

SECTION 12. Ecological Information
Environmental Fate:
When released into the soil, this material is expected to readily
biodegrade. When released into the soil, this material is not expected toevaporate significantly. When released into water, this material isexpected to readily biodegrade. This material is not expected tosignificantly bioaccumulate. When released into the air, this material
may be moderately degraded by reaction with photochemically
produced hydroxyl radicals. When released into the air, this material
may be removed from the atmosphere to a moderate extent by wetdeposition.
Environmental Toxicity:
This material is not expected to be toxic to aquatic life.

SECTION 13. Disposal Considerations
Whatever cannot be saved for recovery or recycling should bemanaged in an appropriate and approved waste disposal facility.
Processing, use or contamination of this product may change the wastemanagement options. State and local disposal regulations may differ
from federal disposal regulations. Dispose of container and unused
contents in accordance with federal, state and local requirements.

SECTION 14. Transport Information
Not regulated.

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SECTION 15. Regulatory Information
Chemical Inventory Status - Part 1
Ingredient
Glycerol (56-81-5)
TRIS (77-86-1)

TSCA EC Japan Australia
Yes Yes Yes
Yes
Yes Yes Yes
Yes

Chemical Inventory Status - Part 2CanadaKorea DSL NDSL Phil.
Yes Yes
No YesYes Yes
No YesIngredient
Glycerol (56-81-5)
TRIS (77-86-1)
Federal, State & International Regulations - Part 1-SARA 302
RQ TPQ
No No
No No

Ingredient
Glycerol (56-81-5)
TRIS (77-86-1)

SARA 313
List Chemical Catg.
No
No
No
No

Federal, State & International Regulations - Part 2
Ingredient
Glycerol (56-81-5)
TRIS (77-86-1)

CERCLA
No
No

RCRA
261.33
No
No

TSCA
8(d)
No
No

Chemical Weapons Convention: No
TSCA 12(b): No
CDTA: NoSARA 311/312:
Glycerol: Acute: Yes
TRIS:Acute: Yes
Reactivity: No

Chronic: Yes Fire: No Pressure: No
Chronic: No Fire: No Pressure: No

Australian Hazchem Code: None allocated.
Poison Schedule: None allocated.
WHMIS:This MSDS has been prepared according to the hazard criteria of theControlled Products Regulations (CPR) and the MSDS contains all of
the information required by the CPR.

SECTION 16. Other Information
NFPA Ratings: Health: 1 Flammability: 1 Reactivity: 0Label Hazard Warning:
CAUTION! MAY CAUSE IRRITATION TO SKIN, EYES, ANDRESPIRATORY TRACT. MAY AFFECT KIDNEYS. WARNING!
HARMFUL IF SWALLOWED OR INHALED. CAUSESIRRITATION TO SKIN, EYES AND RESPIRATORY TRACT.
Label Precautions:
Avoid breathing mist.
Avoid contact with eyes, skin and clothing.
Keep container closed.

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Use with adequate ventilation.
Wash thoroughly after handling.
Label First Aid:
If inhaled, remove to fresh air. Get medical attention for any breathingdifficulty. In case of contact, immediately flush eyes or skin withplenty of water for at least 15 minutes. Get medical attention ifirritation develops or persists. If swallowed, induce vomiting
immediately as directed by medical personnel. Never give anything by
mouth to an unconscious person. If inhaled, remove to fresh air. If notbreathing, give artificial respiration. If breathing is difficult, giveoxygen. In case of contact, immediately flush eyes or skin with plentyof water for at least 15 minutes. Remove contaminated clothing andshoes. Wash clothing before reuse. In all cases, get medical attention.
Product Use:Laboratory Reagent.

Disclaimer:
Transgenomic, Inc. provides the information contained herein in good faith but makes
no representation as to its comprehensiveness or accuracy. This document is intended
only as a guide to the appropriate precautionary handling of the material by a properly
trained person using this product. Individuals receiving the information must exercise
their independent judgment in determining its appropriateness for a particularpurpose. TRANSGENOMIC, INC. MAKES NO REPRESENTATIONS OR
WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING WITHOUT
LIMITATION ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A
PARTICULAR PURPOSE WITH RESPECT TO THE INFORMATION SETFORTH HEREIN OR THE PRODUCT TO WHICH THE INFORMATIONREFERS. ACCORDINGLY, TRANSGENOMIC, INC. WILL NOT BERESPONSIBLE FOR DAMAGES RESULTING FROM USE OF OR RELIANCE
UPON THIS INFORMATION.

Transgenomic, Inc.
12325 Emmet Street
Omaha, NE, USA 68164
Phone: (402) 452-5400
FAX: (402) 452-5401

Transgenomic, Ltd.
UK - Glasgow
40 Watt Road
Hillington Park
Hillington, Glasgow, UK G52 4RY
Phone: +44 (0)141 892 8800
FAX: +44 (0)141 883 5967

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