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Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
Date of issue for the 1st edition : 16/Oct/2013
Date of revision : 04/Jul/2023

Safety Data Sheet

Section 1. Identification of the substance/mixture and of the company/undertaking
Product identifier:Product name: Crystal Violet
Reference number(SDS):27200jis_E-4
Product type：
Reagent
Relevant identified uses of the substance or mixture and uses advised against
Relevant identified uses of the product: Research and Development
Uses advised against: Do not use for other purposes.
Details of the supplier of the safety data sheet
Manufacturer/Supplier: JUNSEI CHEMICAL CO., LTD.
Address: 1-6, Ohmano-cho, Koshigaya-shi, Saitama 343-0844, Japan
Division: Quality Assurance Department
Telephone number: +81-48-986-6161
FAX: +81-48-989-2787
e-mail address: shiyaku-t@junsei.co.jp

Section 2. Hazards identification
GHS classification and label elements of the product
Classification of the substance or mixture
HEALTH HAZARDS
Acute toxicity (Oral): Category 3
Serious eye damage/eye irritation: Category 2
Carcinogenicity: Category 1BSpecific target organ toxicity - repeated exposure: Category 2 (liver, reproductive organs (female))
ENVIRONMENT HAZARDS
Hazardous to the aquatic environment, short-term (acute): Category 1
Hazardous to the aquatic environment, long-term (chronic): Category 1(Note) GHS classification without description: Not classified/Classification not possible
Label elements

Signal word: Danger
HAZARD STATEMENT
H301-Toxic if swallowed
H319-Causes serious eye irritation
H350-May cause cancer
H373-May cause damage to organs through prolonged or repeated exposure
H400-Very toxic to aquatic life
H410-Very toxic to aquatic life with long lasting effects
PRECAUTIONARY STATEMENT
Prevention
Obtain special instructions before use.
Do not handle until all safety precautions have been read and understood.
Avoid release to the environment.
Do not breathe dust/fume/gas/mist/vapors/spray.

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Wash contaminated parts thoroughly after handling.
Wear eye protection/face protection.
Use personal protective equipment as required.
Do not eat, drink or smoke when using this product.
Response
Collect spillage.
Get medical advice/attention if you feel unwell.
IF exposed or concerned: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing.
If eye irritation persists: Get medical advice/attention.
IF SWALLOWED: Rinse mouth. Immediately call a POISON CENTER/doctor/physician.
Storage
Store locked up.
Disposal
Dispose of contents/container in accordance with local/national regulation.

Section 3. Composition/information on ingredients
Mixture/Substance selection:
Substance
Common name, synonyms: Hexamethylpararosaniline chloride; C.I. Basic Violet 3[CI-42555];
Dimethyl(4-{Bis[4-(dimethylamino)phenyl]methylidene}cyclohexa-2,5-dien-1-ylidene)
ammonium chloride
Ingredient name:Crystal Violet
Chemical formula:C25H30ClN3・nH2O(Extra Pure…n=9; Chemical Pure…n=ca. 1.5)
Chemicals No, Japan:4-873; 5-1971
CAS No.:548-62-9(anh)
MW:407.99(anh)
ECNO:208-953-6(anh)Note : The figures shown above are not the specifications of the product.
Components contributing to the hazard
Carcinogenic (Article 57a) in REACH SVHC candidate list
Crystal Violet

Section 4. First-aid measures
Descriptions of first-aid measures
General measures
Get medical advice/attention if you feel unwell.
Call emergency medical service.
IF INHALED
Remove person to fresh air and keep comfortable for breathing.
Give artificial respiration if victim is not breathing.
Administer oxygen if breathing is difficult.
Call a POISON CENTER/doctor/physician if you feel unwell.
IF ON SKIN (or hair）
Take off immediately all contaminated clothing. Rinse skin with water or shower.
If skin irritation or rash occurs: Get medical advice/attention.
Remove and isolate contaminated clothing and shoes.
IF IN EYES
Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy
to do. Continue rinsing.
If eye irritation persists: Get medical advice/attention.

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Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
IF SWALLOWED
Rinse mouth.
Immediately call a POISON CENTER/doctor/physician.
Call a POISON CENTER/doctor/physician if you feel unwell.
Most important symptoms and effects, both acute and delayedSpecific information on symptom and effect are unknown.
Indication of any immediate medical attention and special treatment needed
Ensure that medical personnel are aware of the material(s) involved, and take precautions
to protect themselves.

Section 5. Fire-fighting measures
Extinguishing media
Suitable extinguishing media
In case of fire, use water mist, foam, dry powder, CO2 to extinguish.
Unsuitable extinguishing media
Unsuitable extinguishing media data is not available.
Specific hazards arising from the substance or mixture
Containers may explode when heated.
Fire may produce irritating, corrosive and/or toxic gases.
Runoff from fire control or dilution water may cause pollution.
Advice for firefighters
Specific fire-fighting measures
Evacuate non-essential personnel to safe area.
Special protective equipment and precautions for fire-fighters
Wear fire resistant or flame retardant clothing.
Wear protective gloves/protective clothing/eye protection/face protection.
Firefighters should wear self-contained breathing apparatus with a full facepiece operated
in the positive pressure mode.

Section 6. Accidental release measures
Personnel precautions, protective equipment and emergency procedures
Keep unauthorized personnel away.
In case of contact with substance, immediately flush skin or eyes with running water for atleast 20 minutes.
Ventilate area until material pick up is complete.
Wear proper protective equipment.
Do not touch or walk through spilled material.
Environmental precautions
Avoid release to headsprings, rivers, lakes, ocean and groundwater.
Methods and materials for containment and cleaning up
Absorb or cover with dry earth, sand or other non-combustible material and transfer to containers.
With clean shovel place material into clean, dry container and cover loosely; movecontainers from spill area.
Preventive measures for secondary accident
Collect spillage.
Stop leak if you can do it without risk.
Prevent dust cloud.

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Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
Section 7. Handling and storage
Precautions for safe handling
Preventive measures(Exposure Control for handling personnel)
Do not breathe dust/fume/gas/mist/vapors/spray.
(Protective measures against fire and explosion)
Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
(Exhaust/ventilator)
Exhaust/ventilator should be available.
(Safety treatments)
Avoid contact with skin.
Avoid contact with eyes.
Safety Measures
Obtain special instructions before use.
Do not handle until all safety precautions have been read and understood.
Wear protective gloves/protective clothing/eye protection/face protection.
Use personal protective equipment as required.
When using do not eat, drink or smoke.
Any incompatibilities
Strong oxidizing agents should not be mixed with the chemicals.
Advice on general occupational hygiene
Wash contaminated parts thoroughly after handling.
Do not eat, drink or smoke when using this product.
Storage
Conditions for safe storage
Store in a well-ventilated place. Keep container tightly closed.
Keep cool. Protect from sunlight.
Store in accordance with local/national regulation.
Store locked up.
Container and packaging materials for safe handling data is not available.
Specific end use(s)See information in Section 7.1 and 7.2 for handling and storage recommendations. See
Section 8 for exposure controls and personal protection recommendations.

Section 8. Exposure controls/personal protection
Control parameters
Control value in MHLW is not available.
Adopted value
Adopted value in JSOH is not available.
Adopted value in ACGIH is not available.
Exposure controls
Appropriate engineering controls
Do not use in areas without adequate ventilation.
Eye wash station should be available.
Washing facilities should be available.
Individual protection measures
Respiratory protection
Wear respiratory protection.
Hand protection
Wear protective gloves. Recommended material(s): nitrile
Inspect before use and replace worn or damaged gloves.
Contact the glove manufacturer for specific advice on glove selection and breakthrough5/10
Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
times for your use conditions.
Eye protection
Wear chemical safety goggle.
Wear eye/face protection.
Skin and body protection
Wear impervious clothing and boots in case of repeated or prolonged treatment.

Section 9. Physical and Chemical Properties
Information on basic physical and chemical properties
Physical state: Crystals～crystalline powder
Color: Dark yellow～Dark green
Odor data is not available.
Odor threshold data is not available.
Melting point/Freezing point data is not available.
Boiling point or initial boiling point data is not available.
Boiling range data is not available.
Flammability (gases, liquids and solids) data is not available.
Lower and upper explosion limit/flammability limit data is not available.
Flash point data is not available.
Auto-ignition temperature data is not available.
Decomposition temperature: 215℃
Self-Accelerating Decomposition Temperature/SADT data is not available.
pH data is not available.
Dynamic viscosity data is not available.
Kinematic viscosity data is not available.
Solubility:
Solubility in water: Slightly soluble
Solubility in solvent data is not available.
n-Octanol/water partition coefficient data is not available.
Vapor pressure data is not available.
Vapor density data is not available.
Density and/or relative density data is not available.
Relative vapor density (Air=1) data is not available.
Relative density of the Vapor/air - mixture at 20℃ (Air = 1) data is not available.
Particle characteristics data is not available.
Other information
Critical temperature data is not available.
Evaporation rate data is not available.
VOC data is not available.

Section 10. Stability and Reactivity
Reactivity
Reactivity data is not available.
Chemical stability
Stable under normal storage/handling conditions.
Possibility of hazardous reactions
Possibility of hazardous reactions data is not available.
Conditions to avoid
Contact with incompatible materials.
Open flames. Heating.
Incompatible materials
Strong oxidizing agents

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Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
Hazardous decomposition products
Carbon oxides, Nitrogen oxides, Chlorides

Section 11. Toxicological Information
Information on toxicological effects
Acute toxicity
Acute toxicity (Oral)[Product]Category 3, Toxic if swallowed[Data for components of the product][GHS Cat. Japan, base data](Crystal Violet)
rat LD50=180mg/kg (JECFA FAS69, 2014)
Acute toxicity (Dermal)[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Acute toxicity (Inhalation)[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Irritant properties
Skin corrosion/irritation[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Serious eye damage/irritation[Product]Category 2, Causes serious eye irritation[Data for components of the product][GHS Cat. Japan, base data](Crystal Violet)
human : eyes irritation (HSDB, Access on May 2019)
Sensitization
Respiratory sensitization[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Skin sensitization[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Germ cell mutagenicity[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.

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Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
Carcinogenicity[Product]Category 1B, May cause cancer[Data for components of the product][GHS Cat. Japan, base data](Crystal Violet)
cat.1B; (JECFA FAS69, 2014 et al.)[IARC](Crystal Violet)
Group 2B : Possibly carcinogenic to humans[EU](Crystal Violet)
Category 2; Substances suspected human carcinogens
Reproductive toxicity[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Specific target organ toxicity (STOT)
STOT-single exposure[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
STOT-repeated exposure[Product]Category 2, May cause damage to organs through prolonged or repeated exposure[Data for components of the product][cat.2][GHS Cat. Japan, base data](Crystal Violet)
female genitalia, liver (JECFA FAS69, 2014 et al.)Aspiration hazard[Product]Classification not possible (Insufficient data available or no data available).
[Data for components of the product]
No data available.
Information on other hazards
Reference data : This product's anhydride (CAS No. 548-62-9)

Section 12. Ecological Information
Toxicity
Aquatic toxicity[Product]Category 1, Very toxic to aquatic life
Category 1, Very toxic to aquatic life with long lasting effects[Data for components of the product]
Hazardous to the aquatic environment, short-term (acute)[Company proprietary data](Crystal Violet) Fish(Oryzias latipes) LC50=100μg/kg/48hr (HSDB)

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Crystal Violet,JUNSEI CHEMICAL CO., LTD.,27200jis_E-4,04/Jul/2023
Water solubility[Data for components of the product][Company proprietary data](Crystal Violet) 4g/L(25℃) (HSDB; PHYSPROP Database)
Persistence and degradability
Persistence and degradability data is not available.
Bioaccumulative potential[Data for components of the product][Company proprietary data](Crystal Violet) log Pow=0.96 (PHYSPROP Database)
Other adverse effects
Ozone depleting chemical data is not available.
Additional information
Reference data : This product's anhydride (CAS No. 548-62-9)

Section 13. Disposal considerations
Description of waste residues and information on their safe handling and methods of disposal,
including the disposal of any contaminated packaging
Waste treatment methods
Avoid release to the environment.
Dispose of contents/container in accordance with local/national regulation.

Section 14. Transport Information
UN No., UN CLASS
UN Number or ID Number : 3077
UN Proper Shipping Name : ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.
Class or division (Transport hazard class) : 9
Packing group : III
ERG GUIDE No.: 171
IMDG Code (International Maritime Dangerous Goods Regulations)
UN Number or ID Number : 3077
UN Proper Shipping Name : ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.
Class or division (Transport hazard class) : 9
Packing group : III
IATA (Dangerous Goods Regulations)
UN Number or ID Number : 3077
UN Proper Shipping Name : ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.
Class or division (Transport hazard class) : 9
Hazard labels : Miscellaneous & Environmentally hazardous
Packing group : III
Environmental hazards
Marine pollutants (yes/no) : yes
Transport in bulk according to Annex II of MARPOL 73/78 and the IBC CodeNot applicable to Transport in bulk according to Annex II of MARPOL and the IBC CodeMARPOL Annex V - HME (Harmful to the Marine Environment)
Carcinogenicity: cat.1, 1A, 1B
Crystal Violet

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Section 15. Regulatory Information
Safety, health and environmental regulations/legislation specific for the substance or mixture
List of substances subject to authorisation (REACH, Annex XIV)/SVHC - candidate list
Carcinogenic (Article 57a)
Crystal Violet
U.S. Toxic Substances Control Act (TSCA) Inventory
Chemicals listed in TSCA Inventory548-62-9(anh)All components are listed or exempted.
Other regulatory information
We are not able to check up the regulatory information with regard to the substances inyour country or region, therefore, we request this matter would be filled by yourresponsibility.
Regulatory information with regard to this substance in your country or in your region
should be examined by your own responsibility.
Ensure this material in compliance with federal requirements and ensure conformity to local
regulations.
Regulatory information in this section are limited to intentional ingredient(s), but does
not contain information on non-intentional ingredients or impurities which are not informed
supplier(s).
Chemical safety assessment
Advice on safe handling for this product can be found in sections 7 and 8 of this SDS.

by

Section 16. Other information
GHS classification and labellingH301-Acute toxicity, Category 3: H301 Toxic if swallowed
H319-Serious eye damage/eye irritation, Category 2: H319 Causes serious eye irritation
H350-Carcinogenicity, Category 1B: H350 May cause cancer
H373-STOT - Repeated exposure, Category 2: H373 May cause damage to organs throughprolonged or repeated exposure
H400-Hazardous to the aquatic environment, short-term (acute), Category 1: H400 Very toxic
to aquatic life
H410-Hazardous to the aquatic environment, long-term (chronic), Category 1: H410 Very toxic
to aquatic life with long lasting effects
References and sources for data
Globally Harmonized System of classification and labelling of chemicals, UN
Recommendations on the TRANSPORT OF DANGEROUS GOODS 22nd edit., 2021 UN
IMDG Code, 2020 Edition (Incorporating Amendment 40-20)
IATA Dangerous Goods Regulations (64th Edition) 2023
2020 EMERGENCY RESPONSE GUIDEBOOK (US DOT)
2022 TLVs and BEIs. (ACGIH)
JIS Z 7252 : 2019
JIS Z 7253 : 20192022 Recommendation on TLVs (JSOH)
Notification No. 0111-1 (January 11, 2022), Chemical Hazards Control Division, Industrial
Safety and Health Department, Labour Standards Bureau, MHLW in Japan
Supplier's data/information
Chemicals safety data management system "GHS Assistant" Version 4.22 (https://www.asahi-ghs.com/)
NITE Chemical Risk Information Platform "NITE-CHRIP"(https://www.nite.go.jp/en/chem/chrip/chrip_search/systemTop)
GHS Classification Guidance for Enterprises 2019 Revised Edition (Ver. 2.0) （Mar. 2020, METI)

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Abbreviations and acronyms
SDS (Safety Data Sheet)
LD50 (Lethal Dose, 50％)
LC50 (Lethal Concentration, 50％)
IARC (International Agency for Research on Cancer)
ACGIH (American Conference of Governmental Industrial Hygienists)
EPA (US Environmental Protection Agency)
NTP (US National Toxicology Program)
JSOH (Japan Society for Occupational Health)EU (European Union)
EC50 (Effective Concentration, 50％)
NOEC (No Observed Effect Concentration)
BOD (Biochemical Oxygen Demand)
COD (Chemical Oxygen Demand)
BCF (Bioconcentration Factor)anh (anhydride)
General Disclaimer
This data sheet was created based on the information we currently have and may be revised
according to new information. In addition, the precautions apply only to normal handling,
and in the case of special handling, please make adequate countermeasure to maintain yoursafety.
The data given here is based on current knowledge and experience. The purpose of this
Safety Data Sheet is to describe the products in terms of their safety requirements. The
data does not signify any warranty with regard to the products' properties.
The GHS classification data given here is based on current Japan official data (NITE
published in 2021).
But the data are partially changed based on our judgement.