Joint Usage / Research Center for “Proteointeractome analysis（PRiME）”
Proteo-Science Center, Ehime University
Public Call for Applications for FY2024

The Proteo-Science Center (PROS), Ehime University, promotes the discovery and identification ofprotein interactions based on technologies such as wheat cell-free protein synthesis, protein arrays,
and proximal-dependent labeling enzymes (AirID). The PRiME Joint Usage / Research Center for“Proteo-interactome analysis” provides an integrated collaborative research platform for the analysisof proteins, verifying their functions at the proteome level. PRiME is now accepting applications forjoint use and collaborative research for FY2024 from researchers in Japan and overseas.

1. Publicly Advertised Items(A) Joint Usage (5-10 projects)
PROS/PRiME provides technical support using wheat cell-free protein synthesis technology tosynthesize more than 20,000 total human proteins, and arrays in which these proteins are on glass
slides or in multi-well plates. We can also provide support for analysis of interacting proteins at thecellular and individual level using AirID. We invite applications for joint use from Japan andinternationally, and in principle subsidize travel expenses. In addition, a maximum of 150,000 yen
per case per fiscal year will be subsidized, with any shortfall to be borne by the beneficiaries.
For further details, please contact the following persons;・Protein Arrays
Hiroyuki Takeda: takeda.hiroyuki.mk@ehime-u.ac.jp
Hirotaka Takahashi: takahashi.hirotaka.rn@ehime-u.ac.jp
・AirID
Satoshi Yamanaka: yamanaka.satoshi.ze@ehime-u.ac.jp
Akira Nozawa: nozawa.akira.my@ehime-u.ac.jp

(B) Joint Research (20-30 projects)
PROS/PRiME invites joint research proposals based on the applicant's research, from theunderstanding of basic molecular mechanisms of biology relating to protein interactomics to theelucidation of disease pathology and the development of diagnostic methods and treatments including
drug discovery. In principle, the maximum amount of joint research expenses is 300,000 yen per
proposal per fiscal year. For details, please consult with PROS faculty members.

In addition, this year we are inviting applications for joint use/joint research with the Institute forProtein Research (IPR), Osaka University, including analysis of protein structure, etc., as a special
program. In this special program, the research proposal that effectively utilize both centers will beselected on a priority basis, and preferential measures such as allocation of shared resources (e.g.,machine time) will be given to accepted special program proposals.

※Matching of proposed research with PROS faculty is also possible. If you wish to be matched for ameeting with a PROS faculty member prior to application, please contact Yuuki Imai: yimai@m.ehime-u.ac.jp

2. Eligibility
Researchers (including graduate students) affiliated with faculties or other research institutions atuniversities in Japan or internationally, and those who are approved by the center director. If thePrincipal Investigator is a graduate student, please submit a Letter of Consent (Form 2) from thesupervising professor of his/her laboratory. However, the principal investigator must be a researcher
outside of Ehime University, and a PROS faculty member (whether full-time or adjunct) must beincluded in the research organization as a research subproject leader. We especially support young
and female researchers, and respect diversity in terms of gender, age, nationality, race, etc.

3. How to Apply
After discussion with a PROS faculty member, please fill out the application form (Form 1-A: Joint
Usage or 1-B: Joint Research) and submit it as a PDF file by e-mail to 11 as below.

4. Schedule
Public offering period: December 28, 2023 ~ January 22, 2024 ※strict deadline
Decision on adoption: Late March 2024
Collaborative Research Period: April 1, 2024 ~ March 31, 2025

5. Selection and Notification
The PRiME Steering Committee, will make a decision on acceptance or rejection of the applicationand the amount of research funds to be allocated after comprehensively discussing the details of theapplication (necessity, originality, feasibility, etc. of the proposal). After the decision is made, theapplicant will be notified directly of the selected research proposal by the end of March 2024.

6. Reporting of Outcomes
Please submit the Research Results Report (Form 3) by e-mail to 11 below by March 31, 2025. The
contents of the report will be made public. If you need to temporarily suspend the publication of theresults because you have filed or plan to file a patent application, please submit a Request for
Postponement of Submission of Joint Research Report (Form 4).

7. Publication of Results
If you have published your results in an academic paper or other academic publication, please statein the paper that it is a joint research project of the Center, and submit one reprint (PDF isacceptable) of the published paper to 11 below. If the paper is written in English, please describe in
the "Acknowledgements" column thus:

This study was supported (partly) by the Joint Usage/Research Center for Proteo-Interactome(PRiME), the Proteo-Science Center, Ehime University

In addition, we may hold a debriefing session to report on the results and ask you to make apresentation.

8. Intellectual Property Rights
In the event that intellectual property is created as a result of this joint research, discussions will beheld between the researchers and the department in charge of intellectual property at theorganization to which the relevant joint research organization belongs regarding the applicability,
ownership, procedures, etc.

9. Safety and Security Output Management
If the research organization includes researcher outside Japan, we may request the submission ofmaterials for the relevant determination based on the Foreign Exchange and Foreign Trade Law(Foreign Exchange and Foreign Trade Law), if necessary, taking into consideration the country towhich the researcher belongs and the content of the joint research project in question.

10. Genetic Resources
Please note that with the implementation of the "Guidelines for the Fair and Equitable Sharingof Opportunities for the Acquisition of Genetic Resources and Benefits Arising from their
Utilization" from August 20, 2009, the acquisition of overseas biological samples (geneticresources) and research requires ABS procedures based on the Convention on BiologicalDiversity and the Nagoya Protocol.

11. Where to submit applications and for inquiriesApplicants must submit the application form (Form 1-A or 1-B) and the faculty advisor'sapproval letter (Form 2) as a PDF file by e-mail to the address below. Please download theapplication form and other forms from the PRiME website, below.

https://www.pros.ehime-u.ac.jp/prime

The Third Team, Research Support Division, Research Support Department, Ehime University
3 Bunkyo-cho, Matsuyama, Ehime 790-8577 JAPANemail: prime@stu.ehime-u.ac.jp
TEL: 089-927-9686
FAX: 089-927-8528