Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18102-0807-8
Rockland, ME 04841 USA

NuSieve® GTG® Agarose
The ideal choice for separation and manipulation of PCR products <1,000 bp.
Precautions
Always wear eye protection when dissolving agaroseand guard yourself and others against scalding
solutions. Refer to the Material Safety Data Sheet for
additional safety and handling information.

Introduction
NuSieve® GTG® Agarose is a unique low melting
temperature agarose for resolving DNA fragments from10 bp to 1,000 bp. Testing of GTG® Agarose ensurescompatibility with routine molecular biology techniques.
NuSieve® GTG® Agarose is tested and certified forreliable ligation and transformation of DNA directly inremelted agarose. Cloning procedures can be performed
directly in remelted agarose, eliminating costly and timeconsuming DNA extraction steps.
Analytical Specifications
Gelling temperature (4%)
Melting temperature (4%)
Gel strength (4%)

Microwave lnstructions for Agarose Preparation
1. Choose a beaker that is 2-4 times the volume of thesolution.
2. Add chilled 1X or 0.5X electrophoresis buffer and astir bar to the beaker.
3. Slowly sprinkle in the agarose powder while thesolution is rapidly stirred.
4. Remove the stir bar if not Teflon® coated.
5. Soak the agarose in the buffer for 15 minutes before
heating. This reduces the tendency of the agarose
solution to foam during heating.
6. Weigh the beaker and solution before heating.
7. Cover the beaker with plastic wrap.
8. Pierce a small hole in the plastic wrap for ventilation.
For agarose concentrations >4%, the following
additional steps will further help prevent theagarose solution from foaming during
melting/dissolution:
A. Heat the beaker in the microwave oven on
Medium power for 1 minute.
B. Remove the solution from the microwave.
C. Allow the solution to sit on the bench for 15
minutes.
9. Heat the beaker in the microwave oven on Medium
power for 2 minutes.
10. Remove the beaker from the microwave oven.
Caution: Any microwaved solution may
become superheated and foam over whenagitated.
11. GENTLY swirl the beaker to resuspend any settled
powder and gel pieces.
12. Reheat the beaker on HIGH power until the solution
comes to a boil.
13. Hold at boiling point for 1 minute or until all of theparticles are dissolved.
14. Remove the beaker from the microwave oven.
15. GENTLY swirl the beaker to thoroughly mix theagarose solution.
16. After dissolution, add sufficient hot distilled water to

<35°C
<65°C
>500 g/cm²Applications• PCR† product separation and manipulation• Electrophoresis of DNA fragments <1,000 bp• Preparative DNA and RNA electrophoresis• In-gel PCR†
• In-gel restriction digestion, ligation andtransformation• In-gel cycle sequencing
Suggested Agarose Concentrations
Size RangeFinal Agarose Concentration (%)(Base Pairs)

1X TAE Buffer

1X TBE Buffer

500-1,000
150-700
100-45070-30010-100
8-50

2.5
3.0
3.5
4.0
4.5
5.0

2.0
2.5
3.0
3.5
4.0
4.5

Dye Mobility Table
Migration of double-stranded DNA in relation toBromophenol Blue (BPB) and Xylene Cyanol (XC) inNuSieve® GTG® Agarose Gels.
1X TAE Buffer%

1X TBE Buffer

XC

BPBAgarose

XC

BPB

750
400
115
100
85

175
120
<20<20<202.50
3.00
4.00
5.00
6.00

460
210
150
8050

75
35
<20<20<201

obtain the initial weight.
17. Mix thoroughly.
18. Cool the solution to 50°C-60°C prior to casting.
Hot Plate Instructions for Agarose Preparation
1. Choose a beaker that is 2-4 times the volume of thesolution.
2. Add chilled electrophoresis buffer and a stir bar tothe beaker.
3. Slowly sprinkle the agarose powder while thesolution is rapidly stirred.
4. Weigh the beaker and solution before heating.
5. Cover the beaker with plastic wrap.
6. Pierce a small hole in the plastic wrap for ventilation.
7. Bring the solution to a boil while stirring.
8. Maintain gentle boiling until all the agarose isdissolved (approximately 10 minutes).
9. Add sufficient hot distilled water to obtain the initialweight.
10. Mix thoroughly.
11. Cool the solution to 50°C-60°C prior to casting.

Ordering Information:
Catalog No.
50081
50080
50084

Size
25 g
125 g
500 g

For more information on NuSieve® GTG® Agarose
Contact Technical Service at (800) 521-0390 or visit ourwebsite at www.Lonza.com.

Related Products:
DNA Ladders
DNA Markers
GelStar® Nucleic Acid Gel Stain
AccuGENE® TBE and TAE Buffers
AccuGENE® Molecular Biology Buffers
ß-AgaraseNuSieve® 3:1 Agarose
For Laboratory Use.
†The PCR process may be covered by one or more third-partypatents.

GelStar is a trademark of FMC Corporation. Teflon is atrademark of E.I. duPont de Nemours and Co. All othertrademarks herein are marks of the Lonza Group or its
affiliates. GelStar is covered by U.S. patent 5,436,134. other
U.S. and foreign patents pending.
©2007 Lonza Rockland, Inc.
All rights reserved.