Life Science Education

QX200 Droplet Digital PCR
System for the Classroom™

™

Next-Generation PCR Technology
Droplet Digital PCR (ddPCR™) is a unique digital PCR technologythat provides sensitive detection and absolute quantification oftarget DNA or RNA molecules without the use of standard curves.

Through its Explorer program, Bio-Rad™ offers products at a
discount to educators for use in teaching laboratories. In this way,
students learn modern biotechnology techniques like Droplet
Digital PCR using the same equipment they might someday use
in research and diagnostics labs.
ddPCR Technology and Workflow
Droplet Digital PCR makes PCR “digital” through sample
partitioning and the subsequent statistical analysis of target
detection across the partitions.
The Bio-Rad QX200 System consists of two instruments, theQX200 Droplet Generator* and the QX200 Droplet Reader, andtheir associated consumables. The QX200 Droplet Generator isused to partition ddPCR reaction mix into thousands of nanolitersized droplets. After PCR in a thermal cycler, droplets from eachsample are analyzed individually on the QX200 Droplet Reader.
PCR-positive and PCR-negative droplets are individually counted
to provide absolute quantification of target DNA in digital form.
Alternatively, amplified products can be extracted from droplets
following PCR for downstream applications, such as sequencing
or cloning.
An automated QX200 AutoDG™ Droplet Generator is also available.
Please contact us for ordering details.

Visit bio-rad.com/ExplorerContactUs to request more information.

SampleSimple and reliable, Droplet Digital PCR technology has enabledresearch breakthroughs in cancer biomarker discovery, infectious
diseases, genomic alterations, and gene expression. Its use isincreasing rapidly in the areas of pathogen detection, food testing,and environmental and wastewater monitoring.

OilOilDroplets

12

3 4 5 6 7
DG8™ Cartridge

1Prepare ddPCR
reaction mix

2Generate droplets

3

Perform PCR to amplify
targets in each droplet

4Read and analyze results

8

QX200 Droplet Digital PCR System for the Classroom

Droplet Digital PCR Resources
Visit bio-rad.com/ddPCR for videos, technical reports, published findings, and other resources describing Bio-Rad’sunique Droplet Digital PCR technology and the QX200 ddPCR System.
Ordering Information (Educator Discount)

Catalog #The ordering information listed here is for purchases for educational use only.
Visit bio-rad.com/ddPCR for information on ordering for research use.

ddPCR Reagents

Description

1863026EDU

ddPCR Supermix for Probes, 2 ml (2 x 1 ml), 200 x 20 μl reactions,
2x supermix

1863010EDU

ddPCR Supermix for Probes, 5 ml (5 x 1 ml), 500 x 20 μl reactions,
2x supermix

QX200 Droplet Digital PCR System, includes droplet generator, droplet
reader, computer, software, associated component consumables
QX200 Droplet Generator, includes droplet generator, 1 pkg of24 DG8 Cartridges, 1 pkg of 24 DG8 Gaskets, 2 cartridge holders,
1 power cord
QX200 Droplet Reader, includes droplet reader, ddPCR manual,
2 plate holders, USB cable, power cord
DG8 Cartridges and Gaskets, includes 5 pkg of 24 DG8 Cartridges,
5 pkg of 24 DG8 Gaskets
DG8 Cartridges for QX200 Droplet Generator, 1 pkg of24 cartridges

1863027EDU

ddPCR Supermix for Probes, 25 ml (5 x 5 ml), 2,500 x 20 μl reactions,
2x supermix

1863028EDU

ddPCR Supermix for Probes, 50 ml (10 x 5 ml), 5,000 x 20 μl reactions,
2x supermix1863023EDU

ddPCR Supermix for Probes (No dUTP), 2 ml (2 x 1 ml),
200 x 20 μl reactions, 2x supermix

1863024EDU

ddPCR Supermix for Probes (No dUTP), 5 ml (5 x 1 ml),
500 x 20 μl reactions, 2x supermix

1863025EDU

ddPCR Supermix for Probes (No dUTP), 25 ml (5 x 5 ml),
2,500 x 20 μl reactions, 2x supermix

1863009EDU

DG8 Gaskets for QX200 Droplet Generator, 1 pkg of 24 gaskets

1864021EDU

1863005EDU

Droplet Generation Oil for Probes, 70 ml (10 x 7 ml)

One-Step RT-ddPCR Advanced Kit for Probes, 2 ml (2 x 1 ml), 200 x 20 μl
reactions

1864005EDU

QX200 Droplet Generation Oil for EvaGreen®, 14 ml (2 x 7 ml)

1864022EDU

1864006EDU

QX200 Droplet Generation Oil for EvaGreen®, 70 ml (10 x 7 ml)

One-Step RT-ddPCR Advanced Kit for Probes, 5 ml (5 x 1 ml), 500 x 20 μl
reactions

1863004EDU

ddPCR Droplet Reader Oil, 2 x 1 L

1864033EDU

QX200 ddPCR EvaGreen® Supermix, 2 ml (2 x 1 ml), 200 x 20 μl reactions,2x supermix

1864034EDU

QX200 ddPCR EvaGreen® Supermix, 5 ml (5 x 1 ml), 500 x 20 μl reactions,2x supermix

1864035EDU

QX200 ddPCR EvaGreen® Supermix, 25 ml (5 x 5 ml), 2,500 x 20 μl
reactions, 2x supermix

1864036EDU

QX200 ddPCR EvaGreen® Supermix, 50 ml (10 x 5 ml), 5,000 x 20 μl
reactions, 2x supermix

1863052EDU

ddPCR Buffer Control for Probes, 9 ml (2 x 4.5 ml), 2x buffer

1864052EDU

QX200 Buffer Control for EvaGreen®, 9 ml (2 x 4.5 ml), 2x buffer

12005909EDU

ddPCR Multiplex Supermix, 1.2 ml, (2 x 0.6 ml), 200 x 20 μl reactions,4x supermix

12005910EDU

ddPCR Multiplex Supermix, 3 ml, (5 x 0.6 ml), 500 x 20 μl reactions,
4x supermix

ddPCR SMN1 Copy Number Determination Kit, 200 x 20 µl reactions,
includes 20x SMN1 assay, positive controls, and ddPCR Supermix for Probes(No dUTP) for copy number determination of the SMN1 gene using the QX200
Droplet Digital PCR System

12005911EDU

ddPCR Multiplex Supermix, 12.5 ml, (5 x 2.5 ml), 2,500 x 20 μl reactions,
4x supermix

1864037EDU

ddPCR Supermix for Residual DNA Quantification, 2 ml, (2 x 1 ml),
200 x 20 μl reactions, 2x supermix

ddPCR SMN2 Copy Number Determination Kit, 200 x 20 µl reactions,
includes 20x SMN2 assay, positive controls, and ddPCR Supermix for Probes(No dUTP) for copy number determination of the SMN2 gene using the QX200
Droplet Digital PCR System

1864038EDU

ddPCR Supermix for Residual DNA Quantification, 5 ml, (5 x 1 ml),
500 x 20 μl reactions, 2x supermix

1864039EDU

ddPCR Supermix for Residual DNA Quantification, 25 ml, (5 x 5 ml),
2,500 x 20 μl reactions, 2x supermix

1864040EDU

ddPCR Supermix for Residual DNA Quantification, 50 ml, (10 x 5 ml),
5,000 x 20 μl reactions, 2x supermix

12012171EDU

Dithiothreitol (DTT), 2 ml (2 x 1 ml)

Catalog #Description

QX200 Droplet Digital PCR System
1864001EDU
1864002EDU

1864003EDU
1864007EDU
1864008EDU

Thermal Cycler and Plate Sealer
1851197EDU

1814000EDU
1814040EDU
12001925EDU

C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module,
includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module,USB flash drive
PX1 PCR Plate Sealer, includes heat sealing instrument, plate support blockthat holds 96-well and 384-well plates, sealing frame, power cord
PCR Plate Heat Seal, foil, pierceable, 1 pkg of 100, foil seals for use withPX1 PCR Plate Sealer
ddPCR 96-Well Plates, 1 pkg of 25, clear well/clear shell semi-skirted plates

ddPCR Kits
12013743EDU

1863500EDU

1863503EDU

SARS-CoV-2 ddPCR Kit, 600 tests, includes 2019-nCoV CDC ddPCR Triplex
Assay, One-Step RT-ddPCR Supermix, reverse transcriptase, DTT, positivecontrols and negative controls

BIO-RAD, AUTODG, DDPCR, DROPLET DIGITAL, and QX200 are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.
© 2022 Bio-Rad Laboratories, Inc.
EvaGreen is a trademark of Biotium, Inc. Bio-Rad Laboratories, Inc. is licensed by Biotium, Inc. to sell reagents containing EvaGreen Dye for use in real-time PCR, for research purposes only.
Purchase of Digital PCR and/or Single-Cell NGS Sample Preparation products (the “Products”) from Bio-Rad Laboratories is subject to Bio-Rad Laboratories, Inc. Standard Terms and Conditions of Sale,
which can be accessed at https://www.bio-rad.com/en-us/terms-conditions. Unless we expressly state otherwise in additional Terms and Conditions, no rights are granted for you to distribute or resell theProducts. Unless we expressly state otherwise in additional Terms and Conditions, no rights are granted for the development or commercialization of diagnostic assays for use with the Products without alicense from Bio-Rad. The Products and/or their use are covered by U.S. and foreign patents and/or pending patent applications owned by or under license to Bio-Rad Laboratories, Inc. See https://www.
bio-rad.com/en-us/trademarks.

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