Anti-Influenza B Virus HA antibody, mouse monoclonal (10B8)
Product code65-165

Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerolPurity

Purified IgG fraction with protein A from hybridoma cell culture medium

Immunogen

Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.

Isotypemouse IgG2aκ

Reactivity

According to Ref.1 during epidemic in Osaka 1996-97, 10B8 antibody reacted with HA proteinof all Influenza B virus isolates belonging to Victoria group tested (73 strains) and none ofclinical 27 isolates belonging to Yamagata group as examined by PAP staining. It also reacts
with Victoria group vaccine strains; Shangdong/7/1997, Malasia/2506/2004. However, notethat HA changes during passages and may change reactivity to this antibody. By western
blotting, reactivity with B/Malasia/2506/2004 and B/Massachusetts/2/2012 was tested
positive. No cross reactivity with any strains of influenza A virus.

Special notes

N/A

Application

1. Western blotting (1/500~1/1,000 dilution)
2. Immunofluorescent and Immunocytochemical staining (1/100~1/200 dilution)
3. Immunoprecipitation (1/200 dilution)
4. Neutralization of infectivity (NT) (assay dependent)
5. Hemagglutination Inhibition (HI) (assay dependent)
6. ELISA (assay dependent)

Background

Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface, bringing
about the attachment of the virus particle to the cell. Plays a major role in the determinationof host range restriction and virulence. Class I viral fusion protein. Responsible forpenetration of the virus into the cell cytoplasm by mediating the fusion of the membrane ofthe endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce
an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide.
Several trimers are required to form a competent fusion pore.
Post-translational modificationi
HAo is consists of 584 amino acids with molecular mass of 63,275. In natural infection,
inactive HA is matured into HA1 and HA2. By the sequence similarity it is indicated to bepalmitoylated.

Data LinkUniProtKB P03460 Influenza B/Lee/1940 HA protein.

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-165 Anti-Influenza B Virus HA antibody, mouse monoclonal (10B8)

Fig.1

Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus,

using anti-Influenza B virus HA antibody (clone 10B8).
Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)
efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006
virus (Yamagata group) infected MDCK cells. The cells were ﬁxed with 4% paraformaldehyde in PBSand permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further
reaction with an Alexa Fluor 488-conjugated secondary antibody.
Images on the left are mock-infected MDCK cells as negative control.

Fig. 2 Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B
virus strains by western blotting using 10B8 monoclonal antibody.
1. B/Mie/1/1993
2. B/Johannes Burg/5/1999
3. B/Florida/4/2006
4. B/Lee/1940
5. B/Florida/4/2006
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6. Shandong/7/97
7. B/Malasia/2506/2004
8. B/Massachusetts/2/2012
First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG
antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone
10B8 recognizes an epitope on HA2 region.

References :This product has been used in the following publication
1.Nakagawa N. et al. Rapid detection and identification of two lineages of influenza B strains withmonoclonal antibodies. J Virol Methods. 1999;79:113-2 ICC, IP
2. Nakagawa N. et al. Heterogeneity of Influenza B Virus Strains in One Epidemic Season
Differentiated by Monoclonal Antibodies and Nucleotide Sequences. J Clin Microbiol. 2000;38:34679. HI, NT
3. Nakagawa N. et al. Variation of the Conserved Neutralizing Epitope in Influenza B Virus Victoria
Group Isolates in Japan. J Clin Microbiol. 2005 ;43:4212-4. HI
4. Nakagawa N. et al. Discovery of the neutralizing epitope common to influenza B virus victoriagroup isolates in Japan. J Clin Microbiol. 2006;44:1564-6. HI

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BioAcademia,Inc.

Tel. 81-6-6877-2335

Fax. 81-6-6877-2336

info@bioacademia.co.jp

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