Instructions For Use

FLS-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4

Page 1 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Fite’s Stain Kit(For Leprosy and Nocardia)
Description:

The Fite’s Stain Kit (For Leprosy and Nocardia) is intended for use in the histological visualization ofmycobacterium Lepra bacillus and Nocardia. This kit may be used on formalin-fixed, paraffin-embedded
sections.
Lepra bacillus:
Nocardia:
Background:

RedRedBlue

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

TCS0009-5 (5 Slides)
TCS0009-25 (25 Slides)
Any well fixed paraffin embedded
Nocardia or Lepra bacillus infectedtissue.

Ordering information regarding individual components
on back page!
Kit Contents:
Item #XPO125
CFZ125
AAM500
MBS125

Precautions:

Kit Contents
Xylene-Peanut Oil Solution
Carbol Fuchsin Solution
Acid Alcohol Solution (1%)
Methylene Blue Solution

Volume125 ml
125 ml500 ml125 mlAvoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

25° C
Storage: 18° C
Doc: IFU-TemplateMixedStoragerev2

Storage18-25°C
18-25°C
18-25°C
18-25°C

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CV

P
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For Use

FLS-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4

Page 2 of 3P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Lepra bacillus Procedure (Standard):
1.

Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.

2.

Air dry slide for 15 minutes “without” removing oil film covering tissue section. Remaining film prevents de-staining of
Lepra bacillus during differentiation.

3.

Rinse slide in several changes of distilled water.

4.

Incubate slide in Carbol Fuchsin Solution for 15 minutes.

5.

Rinse slide in several changes of distilled water.

6.

Differentiate section in Acid Alcohol Solution (1%) until background is pink.

7.

Rinse slide in distilled water and check by microscope for correct differentiation.

8.

Rinse in running tap water for 1 minute followed by 1 rinse in distilled water.

9.

Dip slide 2-3 times in Methylene Blue Solution.

10. Dip slide quickly in distilled water and check by microscope for correct staining.
11. Air dry slide at room temperature.
12. Dip slide several times in Xylene or Xylene Substitute.
13. Mount in synthetic resin.

Nocardia Procedure:Preparation of Reagents Prior to Beginning:
1.

Prepare Diluted Acid Alcohol Solution by mixing 25ml of Acid Alcohol Solution (1%) with 25ml of Distilled Water.

Procedure:
1.

Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.

2.

Air dry slide for 15 minutes “without” removing oil film covering tissue section. Remaining film prevents de-staining ofLepra bacillus during differentiation.

3.

Rinse slide in several changes of distilled water.

4.

Incubate slide in Carbol Fuchsin Solution for 15 minutes.

5.

Rinse slide in several changes of distilled water.

6.

Dip slide 2-3 times in Diluted Acid Alcohol Solution.

7.

Rinse slide in distilled water and check by microscope for correct differentiation. Avoid decolorizing the Nocardiaorganism.

8.

Rinse in running tap water for 1 minute followed by 1 rinse in distilled water.

9.

Dip slide 2-3 times in Methylene Blue Solution.

10. Dip slide quickly in distilled water and check by microscope for correct staining.
11. Air dry slide at room temperature.
12. Dip slide several times in Xylene or Xylene Substitute.
13. Mount in synthetic resin.
25° C
Storage: 18° C
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands

Instructions For Use

FLS-1-IFU
Rev. Date: Dec. 7, 2018

Revision: 4Page 3 of 3

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

References:1.

Echeverri, C., et al. Fite Stain Positivity in Rhodococcus equi: Yet Another Acid-Fast Organism in Respiratory Cytology – A Case Report.
Diagnostic Cytopathology; April 2001, Volume 24, Issue 4, pages 244-246.

2.

Crowder, C., Taylor, HW., Modified Fite Stain for Demonstration of Mycobacterium Species in Tissue Sections; Journal ofHistotechnology; 1996, Volume 19; 2: pages 133-134.

3.

Mallory, Pathological Technique; page 275.

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Xylene-Peanut Oil Solution

XPO125
XPO500
XPO999125 ml
500 ml1000 ml

Carbol Fuchsin Solution

CFZ125
CFZ500
CFZ999125 ml500 ml1000 ml

Acid Alcohol Solution (1%)

AAM500
AAM999500 ml1000 ml

Methylene Blue Solution

MBS125MBS500
MBS999125 ml500 ml1000 ml

25° C
Storage: 18° C
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP
Emergo Europe
Prinsessegracht 202514 AP The Hague, The Netherlands