Storage recommendations for samples collected using OMNIgene™
products from DNA Genotek™
The preservation and storage of microbiome samples is an important consideration for any study design.
The table below summarizes recommendations for short-term handling and long-term storageof unprocessed samples collected in the various OMNIgene products offered by DNA Genotek.

OMNIgene•GUT(OMR-200, OM-200)

Room temperature storage(15°C l25°C/59°Fl77°F)

Freezer storage(-20°C or -80°C/-4°F or -112°F)

Storage at room temperature:
Fecal samples collected in OMNIgene•GUT(OMR-200, OM-200) can be stored at room
temperature for up to 60 days.

Storage at -20°C or -80°C (-4°F or -112°F):
Fecal samples collected in OMNIgene•GUT(OMR-200, OM-200) can be stored in their originaltube at -20°C or -80°C (-4°F or -112°F) for up to1 year. For longer storage, OMNIgene•GUTsamples should be vigorously mixed,
aliquoted into cryovials and stored at -80°C (-112°F).

IMPORTANT: Storing at 4°C (39°F) isNOT recommended for fecal samples
collected in OMNIgene•GUT.

Freeze-thaw cycles:
OMNIgene•GUT will preserve the microbialcommunity structure and maintain DNA
integrity for up to 6 freeze-thaw cycles.1

OMNIgene•GUTDNA and RNA(OMR-205)

Storage at room temperature:
Fecal samples collected in OMNIgene•GUT
DNA and RNA (OMR-205) can be stored atroom temperature for up to 10 days.
IMPORTANT: We recommend limiting time
exposure of collected samples to elevatedtemperatures above 37°C (98°F) since these
affect the quality of the RNA.

Storage at -80°C (-112°F):
We recommend storing fecal samples collected inOMNIgene•GUT DNA and RNA (OMR-205) in their
original tube at -80°C (-112°F) as soon as possible.
For long-term storage (> 6 months), we recommend
extracting nucleic acids and storing your extracted
samples as per your standard operating procedures.

Freeze-thaw cycles:
OMNIgene•GUT DNA and RNA will preserve
the microbial community structure andmaintain RNA and DNA integrity for up to3 freeze-thaw cycles.1

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OMNIgene•SALIVAfor DNA and RNA(OMR-610)

Room temperature storage(15°C l25°C/59°F l77°F)

Freezer storage(-20°C or -80°C/-4°F or -112°F)

Storage at room temperature:
Saliva samples collected inOMNIgene•SALIVA DNA and RNA(OMR-610) can be stored at roomtemperature for up to 21 days.

Storage at -20°C or -80°C (-4°F or -112°F):
Saliva samples collected in OMNIgene•SALIVA
DNA and RNA (OMR-610) should be incubated
at 50°C (122°F) for 1 hour in a water bath or
2 hours in a dry incubator, mixed well by inversion
and extracted within 21 days prior to storage at-20°C or -80°C (-4°F or -112°F).

IMPORTANT: Storing at 4°C (39°F)
is NOT recommended for saliva samples
collected in OMNIgene•SALIVA
DNA and RNA.

OMNIgene•ORALfor DNA (OM-501)

Storage at room temperature:
Saliva samples collected in
OMNIgene•ORAL (OM-501) can be stored
at room temperature for up to 1 year.
IMPORTANT: Storing at 4°C (39°F) isNOT recommended for saliva samples
collected in OMNIgene•ORAL.

Freeze-thaw cycles:
OMNIgene•SALIVA DNA and RNA will
maintain DNA AND RNA integrity forup to 3 freeze-thaw cycles.1
Storage at -20°C or -80°C (-4°F or -112°F):
Saliva samples collected in OMNIgene•ORAL(OM-501) should be incubated at 50°C (122°F)
for 1 hour in a water bath or 2 hours in a dryincubator, mixed well by inversion, aliquoted
into cryovials and stored at -20°C (-4°F) or-80°C (-112°F) within 1 year of collection.
Freeze-thaw cycles:
OMNIgene•ORAL will maintain DNA integrity
for up to 3 freeze-thaw cycles.1

OMNIgene•ORAL
for DNA and RNA(OM-505, OME-505)

Storage at room temperature:
Saliva samples collected inOMNIgene•ORAL (OM-505, OME-505)
can be stored at room temperature forup to 21 days.
IMPORTANT: Storing at 4°C (39°F) isNOT recommended for saliva samples
collected in OMNIgene•ORAL.

Storage at -20°C or -80°C (-4°F or -112°F):
Saliva samples collected in OMNIgene•ORAL(OM-505, OME-505) should be incubated at 50°C
(122°F) for 1 hour in a water bath or 2 hours in a
dry incubator, mixed well by inversion andextracted within 21 days prior to storage at-20°C or -80°C (-4°F or -112°F).

Freeze-thaw cycles:
OMNIgene•ORAL will maintain DNA and RNAintegrity for up to 3 freeze-thaw cycles.1

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OMNIgene•ORAL(OMR-110, OMR-120)

OMNIgene•VAGINAL(OMR-130)

Room temperature storage(15°C l25°C/59°F l77°F)

Freezer storage(-20°C or -80°C/-4°F or -112°F)

Storage at room temperature:Samples collected in OMNIgene•ORAL(OMR-110, OMR-120) and OMNIgene•VAGINAL(OMR-130) can be stored at room
temperature for up to 30 days.

Storage at -20°C or -80°C (-4°F or -112°F):
Samples collected in OMNIgene•ORAL(OMR-110, OMR-120) and OMNIgene•VAGINAL(OMR-130) should be treated with Proteinase K
and incubated,2 aliquoted into cryovials and stored
at -20°C or -80°C (-4°F or -112°F) within 30 days ofcollection.

IMPORTANT: Storing at 4°C (39°F) is NOT
recommended for samples collected inOMNIgene•ORAL or OMNIgene•VAGINAL.

IMPORTANT: Ensure samples are vortexed andthe swab has been pressed against the collection
tube’s inner wall to remove excess liquid prior
to aliquoting or if transferring into cryovials.

Freeze-thaw cycles:
OMNIgene•ORAL and OMNIgene•VAGINALwill preserve the microbial community
structure and maintain DNA and RNA
integrity for up to 3 freeze-thaw cycles.1OMNIgene•SKIN (OMR-140)

Storage at room temperature:Samples collected in OMNIgene•SKIN(OMR-140) can be stored at roomtemperature for up to 30 days.
IMPORTANT: Storing at 4°C (39°F) is NOT
recommended for samples collected inOMNIgene•SKIN.

Storage at -20°C or -80°C (-4°F or -112°F):
Samples collected in OMNIgene•SKIN(OMR-140) should be treated with Proteinase Kand incubated,2 aliquoted into cryovials and storedat -20°C or -80°C (-4°F or -112°F) within 30 days ofcollection.
IMPORTANT: Ensure samples are vortexed andthe swab has been pressed against the collection
tube’s inner wall to remove excess liquid prior
to aliquoting or if transferring into cryovials.

Freeze-thaw cycles:OMNIgene•SKIN kits will preserve themicrobial community structure and maintain
DNA integrity for up to 3 freeze-thaw cycles.1

1Freeze-thaw cycle: from -20°C to 30°C (-4°F to 86°F).
2Add 400 μg of Proteinase K to the 1 mL sample collection tube and vortex. Incubate at 50°C for 1 hour in a water bath or 2 hours
in a dry incubator. Ensure that the swab tip is immersed in the solution.
Recommended Proteinase K:•Epicentre® PK (Cat. No. MPRK092) — Add 8 μL of 50 mg/mL stock.
•
QIAGEN® Proteinase K (Cat. No. 19131 or 19133) — Add 20 μL of 20 mg/mL stock.
•Roche Proteinase K (Cat. No. 3115887001, 3115828001 or 3115844001) — Add 22 μL of 18 mg/mL stock.
Technical support is available Monday to Friday (9h00 to 17h00 ET):
Toll-free (North America): 1.866.813.6354, option 6
All other countries: +1.613.723.5757, option 6
Email: support@dnagenotek.com

Some DNA Genotek products may not be available in all geographic regions.
OMNIgene and DNA Genotek are trademarks of DNA Genotek Inc. All other brands and names contained herein are the property of their respective owners.
All DNA Genotek protocols, white papers and application notes are available in the support section of our website at www.dnagenotek.com.
PD-PR-01036 Issue 5/2023-02© 2023 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved.

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