Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18142-0807-04
Rockland, ME 04841 USA

DNA Marker 50-2,500 bpIntroduction

Product Safety:

DNA Markers range in size from 50bp to 2,500 bp for rapid†
size estimation of PCR products and restriction fragments.
Loading 5 μl per lane yields approximately 50 ng of DNA per
band. DNA can be visualized by ethidium bromide staining or32
kinased with radiolabeled P for detection by autoradiography.

For details regarding product safety, see Material Safety Data
Sheet (MSDS); call (800) 638-8174 for extra copies of theMSDS. Emergency after hours, call collect (303) 595-9048.

Warranty:
Because of the numerous factors affecting results, Lonza DNA
markers are sold with the understanding that purchasers will
make their own tests to determine the suitability of thesemarkers for their particular purposes. The use suggested by
Lonza is presented only to assist our customers in exploring
possible applications for this product. All information and data
presented are believed to be accurate and reliable but arepresented without the assumption of any liability by Lonza.

ContentsDNA Marker
250 μl-50 applications
Store at 4°C
Loading Buffer 6X (250 μl)
Contains: bromophenol blue
Store at 4°C or 25°C

References

Triple Dye Loading Buffer 6X (1.1 ml)
Contains: Orange G, bromophenol blue, xylene cyanol
Store at 4°C•Standard Procedure•

Mix 5 μl of DNA marker and 1 μl of 6X loading buffer.
Mix 5 parts of your sample to 1 part of 6X loading buffer.
Load DNA markers and samples onto an agarose gel.
Electrophorese, stain, and photograph following yourstandard protocol.
5. Estimate the size of the sample DNA by reading its
relative position to the closest marker.
NOTE: There is a higher concentration of dye material in theTriple Dye Loading Buffer. Therefore, we recommend it for
use in large (40 cm) gels, run for extended times, (18 hours ormore).
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4.

Procedure for 5΄ End Radiolabeling
NOTE: The marker can be labeled directly or for more efficientlabeling, ethanol precipitate first.

Ethanol Precipitation
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7.

Remove a 100 μl aliquot of the DNA marker.
Add 10 μl of 3 M potassium acetate, pH 7.4.
Add 300 μl of absolute ethanol.
Incubate at -70°C for 30 minutes.
Microcentrifuge at 4°C for 10 minutes.
Redissolve the pallet in 100 μl of distilled water.
Quantitate by reading the absorption at 260 nm and labelwith [γ-32P]-ATP using T4 polynucleotide kinase and astandard protocol. See Sambrook, et al., 5.68 (1989) or
Ausubel, et al. (1987).

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Ausubel, F.M., Brent, R., Kingston, R.E., Moore,D.D., Seidman, J.G., Smith, J.A., and Struhl, K.
Current Protocols in Molecular Biology,
John Wiley & Sons, New York 1987.
Sambrook, J., Fritsch, E.F., and Maniatis, T.
Molecular Cloning, A Laboratory Manual,
Second Edition, Cold Spring Harbor:
Cold Spring Harbor Laboratories 1989

Ordering Information
Catalog No.
50631

Description
DNA Marker 50-2,500 bp

Size
50 Applications

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®
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For more information contact Technical Service at(800) 521-0390 or visit our website at www.Lonza.com.

Manufactured for Lonza Rockland, Inc.
For Research Use Only.
Not for Use in Diagnostic Procedures.
†The PCR process may be covered by one or more third-party
patents.

GelStar and SeaKem are trademarks of FMC Corporation. Allother trademarks herein are marks of the Lonza Group or its
affiliates. GelStar is covered by U.S. patents 5,436,134. OtherU.S. and foreign patent pending. SYBR is a trademark ofMolecular Probes.
© 2007 Lonza Rockland, Inc.
All rights reserved.

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