Lonza Rockland, Inc.
www.lonza.com
scientific.support@lonza.com
Scientific Support: 800-521-0390
Customer Service: 800-638-8174
Document # 18137-0512-05
Rockland, ME 04841 USA

DNA Marker 1–10 kb
Introduction
DNA Markers range in size 1 kb to 10 kb for rapid size
estimation of PCR† products and restriction fragments.
Loading 5 l per lane yields approximately 50 ng of DNA per
band. DNA can be visualized by ethidium bromide staining or32
kinase with radiolabeled P for detection by autoradiography.

ContentsDNA marker
2 x 250 l – 100 applications
Store at 4C6X loading buffer (250 l)
Contains bromophenol blue
Store at 4C or 25C
NOTE: Mix small amounts as needed or mix a large amount,aliquot and store at 4C.

Procedure – Ethidium Bromide Staining
1. Mix 5 l of DNA marker and 1 l of 6X loading buffer.
2. Mix 5 parts of your sample to 1 part of 6X loading buffer.
3. Load DNA markers and samples onto an agarose gel.
4. Electrophorese, stain and photograph following yourstandard protocol.

5. Estimate the size of the sample DNA by reading its
relative position to the closest marker.

Procedure for 5΄ End Radiolabeling
NOTE: The marker can be labeled directly or for more
efficient labeling, ethanol precipitate first.

Ethanol Precipitation
1.
2.
3.
4.
5.
6.
7.

Remove a 100 l aliquot of the DNA marker.
Add 10 l of 3M potassium acetate, pH 7.4
Add 300 l of absolute ethanol.
Incubate at -70C for 30 minutes.
Microcentrifuge at 4C for 10 minutes.
Redissolve the pellet in 100 l of distilled water.
Quantitate by reading the absorption at 260 nm and label-32
with [  P ]-ATP using T4 polynucleotide kinase and astandard protocol. See Sambrook. 5.68 (1989) orAusubel, et al., 3.4.3 (1987).

1Product Safety:
For details regarding product safety, see Material Safety Data
Sheet (MSDS); call (800) 638-8174 for extra copies of theMSDS. Emergency after hours, call collect (303) 595-9048.

Warranty:
Because of the numerous factors affecting results, Lonza DNA
markers are sold with the understanding that purchasers will
make their own tests to determine the suitability of thesemarkers for their particular purposes. The use suggested byLonza, is presented only to assist our customers in exploring
possible applications for this product. All information and data
presented are believed to be accurate and reliable but arepresented without the assumption of any liability by Lonza.

References
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith,J.A., Seidman, J.G., and Struhl, K.
Current Protocols in Molecular Biology,
John Wiley & Sons, New York 1987.
Sambrook, J., Fritsch, E.F., and Maniatis, T.
Molecular Cloning, A Laboratory Manual,
Second Edition, Cold Spring Harbor:
Cold Spring Harbor Laboratories 1989

Ordering Information
Catalog No. Description

Size50471

100 Applications

DNA Marker 1-10 kb

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Reliant™ Gel System®
SYBR Green Gel Stains®
SeaKem LE Agarose™
™
SeaPlaque GTG Agarose™
SeaPlaque Agarose
For more information contact Scientific Support at(800) 521-0390 or visit our website at www.lonza.com.

Manufactured for Lonza Rockland, Inc.
For Research Use Only.
Not For Use In Diagnostic Procedures.
†The PCR process may be covered by one or more third-party
patents.
GelStar and SeaKem are trademarks of FMC Corporation. Allother trademarks herein are marks of the Lonza Group or its
affiliates. GelStar is covered by U.S. patent 5,436,134. Other
U.S. and foreign patent pending. SYBR is a trademark of
Molecular Probes.
2012 Lonza Rockland, Inc.
All rights reserved.

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