Life Science Education

Solutions for Teaching
Real-Time PCR

Visit explorer.bio-rad.com for more information.

PREPARE YOUR STUDENTS FOR
THE FUTURE OF BIOTECHNOLOGY
Real-time PCR, also called quantitative PCR (qPCR), allows simultaneous
amplification and quantification of a target DNA molecule. It is an extremely
powerful technology for not only determining which DNA is present but also
how much.
The use of real-time PCR in research and diagnostics is accelerating, as it offersa streamlined, sensitive approach to determining levels of pathogens in tissues
and in wastewater, detecting aneuploidies, diagnosing other genetic diseases,
and monitoring microbial contaminants in environmental samples, among manyother applications. Teaching students this robust technology prepares them fora future in all areas of life science — basic research, biotechnology, medicine,
food analysis, diagnostics, and more.

Bio-Rad Can Help
An industry leader in the development of cutting-edge products for biologicalresearch and diagnostics, Bio-Rad expresses its commitment to scienceeducation through its Explorer program, which offers Bio-Rad products at a
discount for use in classroom laboratories. This ensures students learn modern
biotechnology techniques like real-time PCR using the same equipment theymight someday use in research and diagnostics labs. The Explorer program
also partners with teachers and industry experts to develop educational kits andtraining resources to help you achieve your teaching goals.

Educational Kits for Real-Time PCR
These interactive lab activities involve students in real-world scenarios and provide instruction on the principles and methods of real-time
PCR. By examining the effects of pipetting and other variables on sensitivity and accuracy, generating standard curves, and analyzing meltcurves, students gain a practical understanding of the unique benefits and applications of both real-time and conventional endpoint PCR.

Crime Scene Investigator PCR Basics
Real-Time PCR Starter Kit
An excellent starting point for novices, thiskit uses premade DNA samples to teach thebasics of real-time PCR. This eliminates thevariables associated with DNA extraction andsample preparation.

2 | BIO-RAD LABORATORIES, INC.

GMO Real-Time PCR Starter Kit
Teach the entire real-time PCR workflow,from DNA extraction from food items todata analysis, as you quantify the amount ofgenetically modified ingredients.

Research-Quality Instruments and Software for Real-Time PCR
CFX Real-Time PCR Systems
CFX Real-Time PCR Systems are used in research and diagnostics laboratories around the world. They offer the performanceand reliability you expect from Bio-Rad, packaged in sleek, modern instruments that fit well into any classroom laboratory.
The CFX Opus and CFX Duet Systems feature:■Quick system setup — easy installation and factory-calibrated optics■

Excellent thermal uniformity and accuracy■

Uniform optical system free of regular calibration requirementsCFX Opus 96 Real-Time PCR System

CFX Opus 384 Real-Time PCR System

CFX Duet Real-Time PCR SystemFeatures

For multiplex qPCR or when wirelessconnectivity is desired

For multiplex qPCR or when wirelessconnectivity is desired

For singleplex or duplex qPCR
applications when wireless connectivity
is not needed

Wells

9638496

Multiplex channels

5 plus FRET

4 plus FRET

2 plus FRET

Stand-alone operation

Yes

Yes

No; requires connection to a PC running
CFX Maestro Software (not included)

Wireless connectivity

Yes

Yes

NoReaction volume

1–50 µl1–30 µl1–50 µl

FRET, fluorescence resonance energy transfer.

CFX Maestro Software — Real-Time PCR Data Collection, Analysis, and Statistics
Use CFX Maestro Software to set up experiments, select reference genes, performstatistical analyses, and create high-resolution, publication-quality graphs from yourresults. This software tool is designed to work seamlessly with CFX Opus Systems
and is required to run the CFX Duet System.

Email
USB Drive

BR.io Cloud
Platform

Work When and Where You Want
Need flexibility? The CFX Opus Systems offer stand-alone operation in addition toEthernet and wireless network (WiFi) connectivity. Save your data to a USB memory
device, connected computer, or networked file folder. You can also program the systems
to send your data or any notifications directly to your email or to a local network for easy
access from other locations. The CFX Opus Systems also connect to the Bio-Rad BR.io
cloud platform, which allows you to set up experiments and retrieve data remotely — yourdata are stored directly in the cloud, where they can be viewed and analyzed anywhere
you have Internet access.
Visit BR.io for more information about the cloud platform.

NetworkedStorage

WiFi

BIO-RAD LABORATORIES, INC. | 3

Have Questions? Need a Quote?
Visit explorer.bio-rad.com to learn more about products and resources for educators.
Contact us at info.bio-rad.com/ExplorerContactUs with any other questions or to request a quote.
Ordering Information (Educator Discount)
The ordering information listed here is for purchases for educational use only.
Visit bio-rad.com/qPCR for information on ordering for research use.
Catalog #Description

Educational Kits for Real-Time PCR
1662660EDU
Crime Scene Investigator PCR Basics Real-Time PCR
Starter Kit, contains sufficient materials for 8 student workstations(2–4 students per workstation)1662560EDUGMO Real-Time PCR Starter Kit, contains sufficient materials for
8 student workstations (2–4 students per workstation)
CFX Real-Time PCR Systems
12016265EDU CFX Duet Real-Time PCR System, includes power cord, USBcable. Order CFX Maestro Software separately​
12011319EDUCFX Opus 96 Real-Time PCR System, includes CFX Opus
96 System and communication cables. Order CFX Maestro
Software separately
12011452EDU CFX Opus 384 Real-Time PCR System, includes CFX Opus
384 System and communication cables. Order CFX Maestro
Software separately
12013346EDU CFX Opus WiFi Adapter, enables WiFi connection for CFX Opus
Systems in North America and other specific countries
CFX Maestro Software
12013758EDU CFX Maestro Software Version 2.3, compatible with Windows
7 and 10, includes USB installation drive, video quick guides, PDF
instruction manual, license for qbase+ Software
PX1 PCR Plate Sealer
1814000EDU
PX1 PCR Plate Sealer, includes heat sealing instrument, plate
support block that holds 96-well and 384-well plates, sealing
frame, power cord
1814040EDUPCR Plate Heat Seal, foil, pierceable, 1 pkg of 100, foil seals
for use with PX1 PCR Plate Sealer

HSP9601EDU

Hard-Shell™ 96-Well PCR Plates, pkg of 50, low profile, thin wall,
skirted, rigid, white shell/clear well, 2-component design
Microseal™ 'B' Adhesive Seals, pkg of 100, optically clear seal
for PCR plates used in PCR and real-time PCR reactions

MSB1001EDU

Reagents for Real-Time PCR
1708880EDU

iQ SYBR Green Supermix, 2.5 ml (2 x 1.25 ml), 2x qPCR mix,
contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I,enhancers, stabilizers, fluorescein; for 100 x 50 µl reactions

1725270EDUSsoAdvanced Universal SYBR® Green Supermix,2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, Sso7d fusion
polymerase, MgCl2, SYBR® Green I, ROX normalization dyes; for200 x 20 µl reactions

Catalog #Description

Reagents for Real-Time PCR (cont.)
1725271EDU

SsoAdvanced Universal SYBR® Green Supermix, 5 ml (5 x 1 ml),
2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2,SYBR® Green I, ROX normalization dyes; for 500 x 20 µl reactions

1725280EDU

SsoAdvanced Universal Probes Supermix, 2 ml (2 x 1 ml),
2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2,stabilizers, ROX normalization dyes; for 200 x 20 µl reactions

1725281EDU

SsoAdvanced Universal Probes Supermix, 5 ml (5 x 1 ml),
2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2,stabilizers, ROX normalization dyes; for 500 x 20 µl reactions

1725282EDU

SsoAdvanced Universal Probes Supermix, 10 ml (10 x 1 ml),
2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2,stabilizers, ROX normalization dyes; for 1,000 x 20 µl reactions

Reagents for Reverse Transcription and One-Step RT-qPCR
1708890EDU

iScript cDNA Synthesis Kit, includes 5x reverse-transcription
reaction mix, iScript reverse transcriptase, nuclease-free water;for 25 x 20 µl reactions

1708891EDU

iScript cDNA Synthesis Kit, includes 5x reverse-transcription
reaction mix, iScript reverse transcriptase, nuclease-free water;for 100 x 20 µl reactions

1708896EDU

iScript Select cDNA Synthesis Kit, includes 5x reaction mix,
iScript reverse transcriptase, oligo(dT), random primer mix,
gene-specific primer enhancer solution, nuclease-free water;for 25 x 20 µl reactions

1708840EDU

iScript Reverse Transcription Supermix, 100 µl 5x supermix,contains reverse transcriptase, RNase inhibitor, dNTPs, primers,MgCl2, stabilizers; 50 reactions of no-RT control supermix;
for 25 x 20 µl reactions

1708898EDU

iScript RT-qPCR Sample Preparation Reagent, 10 ml, contains
RNA stabilizers; for 100 reactions

1725034EDU

iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 µl reactions,
100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor,dNTPs, primers, MgCl2, stabilizers; 200 µl no-RT control supermix;
12.5 µl DNase; 150 µl DNase buffer

12010176EDU

Reliance One-Step Multiplex RT-qPCR Supermix,1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains
Reliance Reverse Transcriptase, blend of high-performance DNApolymerases including Sso7d fusion polymerase, universal referencedye, stabilizer, and reaction buffer; for 200 x 20 μl reactions®

BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc. incertain jurisdictions. All trademarks used herein are the property of their respective owner.
© 2024 Bio-Rad Laboratories, Inc.
SYBR is a trademark of Thermo Fisher Scientific Inc. Bio-Rad Laboratories, Inc. is licensed
by Thermo Fisher Scientific Inc. to sell reagents containing SYBR Green I for use in real-time
PCR, for research purposes only.

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