Bio-Plex Multiplex Immunoassay System

Bio-Plex™ Pro Multiplex Immunoassays:
Tools for Biomarker Discovery and Therapeutic Research and Development
Adding TM symbol per current guidelines.

Visit bio-rad.com/BioPlexBiopharma for more information.

Bio-Plex Pro Multiplex Immunoassays
Use Bio-Plex Pro Assays, Instruments, and Software in early drug discovery,
from target and biomarker screening all the way through to clinical trials.
Whether you want to cast a wide net with a large screening panel or narrow
down to only a handful of key assays, Bio-Plex products, based on Luminex
bead-based xMAP technology, are offered in flexible configurations verified
and validated for specificity, sensitivity, accuracy, and reproducibility, andwith a wide dynamic range.
■■■

 ave precious time and sample while generating
S
actionable results in <3 hours■

 treamline data analysis with Bio-Plex Manager
S
Software’s standard curve auto-optimization andassess relevant differences across multiple analytes andsample types quickly with Bio-Plex Data Pro Software
 ave confidence in your results with high-quality
HBio-Plex Pro Assays manufactured in an ISO
13485:2016–certified facility ■ ssess instrument performance with Installation
AQualification and Operational Qualification performed
by a field service engineer and validation andcalibration tools provided for routine laboratory use nable compliance with U.S. FDA 21 CFR Part 11
E
regulations with Bio-Plex Manager Software,
Security Edition

Increase Efficiency
Discover more in less time with multiplex immunoassays. Bio-Plex Assays utilize beads with unique spectral
addresses determined by their dye ratios. These beads are coupled to capture antibodies, then serially incubatedwith samples, detection antibodies, and a fluorescent reporter, enabling measurement of up to 100 different
proteins in one well in less than 3 hours.
Learn more at bio-rad.com/Bio-PlexAdvantages.
120300 min
311222830 min

57
990
3060 min123

1
2

Workflow overview for Bio-Plex Assays. Stopwatches show the time required for each step (hours. A full assay can be completed in less than three hours ( ).

BIO-RAD LABORATORIES, INC. | 2070 min
3115 min
112= IL-1b= IL-6

2857992) and time elapsed (= IFN-g= TNF-a), where numbers 0–3 represent

Bio-Plex
Multiplex System
196-well plate<3hours

What Would It Take to Measure:x
48 cytokines

Enzyme-Linked
Immunosorbent
Assay (ELISA)≤12.5 μl
serum or plasma48
96-well plates>106hours**

50 μl cell
culture supernatant=38 samples*

1,824 data points>1 ml***
serum or plasma>1 ml*** cell
culture supernatant

Samples run inrun
duplicate.
* * Samples
in duplicate.
** Calculated as 2.2 hours required per plate.
*****Calculated2.2usedhours
required per plate.
Assumes 50 μl ofassample
per well.
*** Assumes 50 μl of sample used per well.

Use Less Sample and Time
Measure multiple proteins using as little as 10 μl of
precious sample. This reduces the number of plates,
reagents, and overall time to results, thereby removing
confounding variables that affect data quality.
Learn more at
bio-rad.com/MultiplexImmunoassays.

Built-In Quality
Bio-Plex Assays are developed using SMARTS
design, which builds in quality from material
procurement to verification and validation of highly
specific, sensitive, and reproducible assays. Highsample detectability in relevant matrices enables
actionable results.
Learn more at bio-rad.com/Bio-Plexsmarts.

SMARTS

Sensitivity
Optimized todetect low-level
endogenous
analytes.

Multiplexibility
Rigorous crossreactivity testing
ensures panels
include only highly
specific assays.

Actionable
Results
Accurate andprecise assays withbiologically relevant
working ranges.

Reproducible
High intra-assay
and inter-assay
precision.

Time to Results
High-quality
antibodies enable
short time toresults for multiple
analytes.

Specificity
In-housedevelopment ofhighly specificantibodies formultiplex assays.

Multiplex Suspension Arrays

NF-kb p65

IL-19

TGF-b1

EGFR

c-Jun

Akt

IL-17A/F
Stat1

IFN-b

IGF-IR

IL-12p70

c-Jun

PDGFR-a

Bio-Plex Multiplex Immunoassays
TGF-b1Analyte Guide 2020

c-Jun
GSK-3a/b

JNK
Erk1/2p70 S6 K

x-Plex Assay

Express Assay

We MixYou Mix

Assay Search Function
Identify your assay of interest in different panels for human, mouse,rat, and multiple species studied in immunology, inflammation, cellsignaling, and disease research.
Learn more at bio-rad.com/FindMyAssay.
Analyte Guide
Reference assay and panel performance parameters along withsample data.
Learn more at bio-rad.com/AnalyteGuide.
Flexible Formats
Only want to measure 7 of those 48 targets? Enjoy the flexibility ofconfiguring your own set of targets in a panel. Build a custom assay orchoose ready-to-order singleplex assays to mix yourself.
Learn more at bio-rad.com/AssayBuilder.

BIO-RAD LABORATORIES, INC. | 3

Your Custom Assay Development Workflow Source
Couple Luminex Beads

Count Beads

Run AssayAmine
Coupling KitTC20 Automated
Cell Counter

Bio-Plex Pro Assay
Reagent Kit

Wash

Automated
Bio-Plex Pro
Wash Station

Increase Precision
Custom Assays
Do you have issues withDo you need to develop yourprecision? Magnetic washingown unique assays? We provide
and automation improve assay
all the tools you need to develop
reproducibility.
and run your assays, including
Learn more atamine coupling kit, antibodies,bio-rad.com/WashStation.
equipment, and an optimized
reagent kit.
Learn more at
bio-rad.com/AssayDevelopment.

Our professional, knowledgeable, andexperienced technical support and serviceengineers are committed to quality and service.
From research to controlled environments,
your Bio-Rad specialists will help care for yourinvestment and keep your projects in motion.
Learn more at bio-rad.com/ExpertCareService.

Manual
Bio-Plex Handheld
Magnetic Washer

ReadAnalyze

Bio-Plex Systems

Bio-Plex Software

Confidence in Your Results
Need confirmation that yourinstrument is performing within
specification? Use our readyto-go Calibration Kit daily andValidation Kit monthly. Bio-Plex
Manager Software, Security
Edition, enables U.S. FDA 21
CFR Part 11 compliance.
Learn more at
bio-rad.com/Bio-PlexManager.

Actionable Data
How do you make sense ofyour data? Quickly compare
samples and identify relevant
differences for targets with
Bio-Plex Data Pro Software.
Learn more at
bio-rad.com/DataPro.

Best-in-Class Service
Bio-Rad has more than two decades of experience with xMAP
technology. Our field service engineers perform IQ/OQ during
instrument installation. Choose from flexible service options tomaintain your instrument.
Experts in the Lab
Need training? Our field application scientists can assist you.
Excellent Support
Have an inquiry? Our technical support team is ready toanswer your questions about Bio-Plex Pro Assays, Instruments,and Software.
Ready to experience the benefits of Bio-Plex Pro Assays?
Learn more at bio-rad.com/Bio-Plextwas.

Visit bio-rad.com/BioPlexBiopharma for more information.
BIO-RAD and BIO-PLEX are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions. xMAP and Luminex are trademarks of Luminex Corporation. All trademarks used herein
are the property of their respective owner. © 2024 Bio-Rad Laboratories, Inc.
The Bio-Plex Suspension Array System includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

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