Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18159-1007-5
Rockland, ME 04841 USALatitude® HT Precast Gels
Ready-to-use agarose gels for high-throughput DNA electrophoresis.
Instructions
1. Cut the pouch open on one end and remove thegel. Exercise caution when removing the gel as
the pouch contains a small amount of bufferthat may contain ethidium bromide.

Introduction
Latitude® HT Precast Gels are designed for fast,accurate high-throughput DNA analysis. Latitude® HTGels are manufactured from high-quality, Lonza
Rockland, Inc. molecular biology grade SeaKem® andNuSieve® Agarose and are precision cast for
reproducible results every time.

2. Leave the gel in the tray and place directly on thechamber platform.

®

Latitude HT Gels fit most large electrophoresis
chambers. We recommend the following systems:••••

3. Cover the gel with the appropriate buffer (1X TAEor 1X TBE) to a depth of 2 mm-3 mm over the gel
surface.

OWL Millipede™ Horizontal SystemOWL Centipede™ Horizontal System(24 x 14 cm gels only)
Bio-Rad® Sub-Cell™ Models 96 and 192 systems
FisherBiotech™ Wide-Format Horizontal System
FB-SB-2318

4. Place the appropriate size Lonza TruBand® Anchor
over the tray so that the white gaskets fit snugly on
the flanges on the short ends of the tray. Position
the anchor so that both the top and middle tiers ofwells are exposed.

Read Before Following the Protocol•LOAD
with TruBand® Anchor

CAUTION!: These gels may contain 0.5 μg
ethidium bromide/ml. Ethidium bromide is a
powerful mutagen. Wear suitable protective
clothing and safety glasses, and refer to MSDS forsafety precautions. Always wear gloves when
opening pouches and handling gels and any solutions
containing ethidium bromide. Decontamination ofethidium bromide is described in Sambrook et al.,
Molecular Cloning, A Laboratory Manual (1989),
6.16-6.17.
Avoid skin and eye exposure to UV light.

•••

5. Add buffer until the level is even with the top of theanchor. Very Important! Do not flood buffer
over the top of the anchor. It is better to have thebuffer level slightly below the top of the anchor thaneven with the top of the anchor.

Do not drop Latitude® HT Gels as the wells willcrack.
For best band resolution, add 0.5 μg ethidium
bromide per ml to the running buffer.
Latitude® HT Gels must be used with theTruBand® Anchor to achieve optimal results.
See diagram on right. Some chambers such as theOWL Millipede A6, the OWL Gator™ A3-1 and theFisherBiotech FB-SB-2318 require a narrow
TruBand® Anchor. Lonza can also provide
platform extensions to improve the gel fit for yourexisting chamber. Contact Lonza Technical
Service for details (800) 521-0390.

6. Load DNA samples following standard procedures.
The gels are configured for alternate orconsecutive-well loading using an 8 or 12 tip multichannel pipette.

1

7. Shift the TruBand® Anchor so that it is centeredover the gel and all wells are completely
covered before beginning electrophoresis.
Related products

AccuGENE® TAE and TBE Buffers
DNA Markers
DNA Ladders

RUNwith TruBand® Anchor
8. Electrophorese the gel at 5-10 V/cm interelectrodedistance, until the bromophenol blue is about 1 cm
from the bottom of the gel or from any row of wells.
9. Remove the anchor, and remove the gel tray fromthe chamber. Carefully remove the gel from thetray and place on the UV light box. Photograph asusual. If the smaller DNA fragments are notdetected, briefly stain the gel (10 to 15 minutes) in
0.5 µg/ml ethidium bromide.

Product Information
Call Lonza Technical Service at (800)-521-0390 for
ordering information or visit www.Lonza.com.
Gels contain 0.5 µg/ml ethidium bromide and are offered
5 gels/box in the following configurations:•1X TBE or 1X TAE
1%, 2%,3.6% or 4% agarose•24x14 cm, 2x50 wells, 10-12 µl/well, alternate well•multichannel pipette loading
24x14 cm, 4x50 wells, 10-12 µl/well, alternate well•multichannel pipette loading
24x20 cm, 6x50 wells, 10-12 µl/well, alternate well•multichannel pipette loading•
24x14 cm, 4x25 wells, 25 µl/well, consecutive wellmultichannel pipette loading•
24x14 cm, 8x25 wells, 25 µl/well, consecutive wellmultichannel pipette loading
Catalog No.

Chamber

For Laboratory Use.

®

TruBand Anchor for 24x20 cm gels
56998
For Owl Millipede™
56992
For Other standardchambersSeaKem is a trademark of FMC Corporation. Centipede, Millipede
and Gator are trademarks of Owl Separation Systems.
FisherBiotech is a trademark of Fisher Scientific. Bio-Rad andSub-cell are trademarks of Bio-Rad Laboratories, Inc. All othertrademarks herein are marks of the Lonza Group or its affiliates.
U.S. patent pending.

TruBand® Anchor for 24x14 cm gels
56991
Owl Millipede
Shelton JBS-96
Fisher SB-2318
56993© 2007 Lonza Rockland, Inc.
All rights reserved.

Other standardchambers2