Cannabis Testing

Cannabis and Hemp Microbial Testing
Enrichment, Detection, Confirmation, Identification, andAutomation Solutions for Microbial Contaminant Analysis

Visit bio-rad.com/cannabis for more information.

Microbial Testing Using Real-Time PCR
Kits, Instruments, Software, and Reagents
iQ-Check™ Real-Time PCR Kits
Validated for a broad range of microbial targets and cannabis
matrices by AOAC, iQ-Check Real-Time PCR Assays have served
cannabis laboratories since the inception of testing regulations.
Key features:■

Real-Time PCR Instrument and Software
The CFX Opus Deepwell Real-Time PCR System is the next
evolution in qPCR from Bio-Rad.
CFX Opus Deepwell Real-Time PCR System

Sensitive 5-color multiplex performance
Flexible connectivity for data management and instrument control■ Open platform■

DNA primers and patented fluorescent double-stranded probes
to ensure specificity to target organism■

Internal amplification controls for PCR inhibitor monitoring■

iQ-Design Assays for optional speciation of Aspergillus spp.

■

CFX Manager Software IDE

Easy-to-use sample setup
Secure and customizable reports■ Ability to use open software for other laboratory tests■■

Easy-to-Use Protocol

12 3 4RESEARCH
INSTITUTE

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Deepw
Systeme PCR
CFX Opus
Real-Tim

12ABell
Deepw
Systeme PCR
CFX Opus
Real-TimC

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Enrich sample.

Extract DNA.

Set up PCR.

Run real-time PCR, view results.

Automation with the iQ-Check Prep System
The iQ-Check Prep System fully automates DNA extraction and PCR setup, detection,
and analysis with the most integrated PCR automation solution on the market.
Benefits:

Run a few or as many as 500 tests in an 8 hr shift
Reduce hands-on time with ready-to-use, load-and-go reagents■ Improve traceability, maximize reproducibility■ Avoid cross-contamination■■iQ-Check Prep System

iQ-Check Real-Time PCR Kit

Free DNA Removal
iQ-Check Free DNA Removal Solution (FDRS) is a
noncarcinogenic, enzymatic treatment to remove free
DNA from dead cells, which could otherwise generate
ambiguous PCR data. FDRS has been demonstrated tohave no impact on intact target cells and is part of theAOAC-approved iQ-Check workflow.

Free DNALive Cells

Free DNA Removal Solution

Free DNA RemovedWWW

PCR Inhibition Removal
iQ-Check Purification Reagent offers a polymer-mediated treatment of extracted DNA to sequester PCR-inhibitory
compounds such as polyphenols to provide a simple and effective approach for sample purification.

2 | BIO-RAD LABORATORIES, INC.

Microbial Testing Using Chromogenic MediaRAPID'Chromogenic Media

E. coli/Coliforms

E.coli O157:H7

Enterobacteriaceae

Listeria

Salmonella

Staphylococcus

RAPID'Chromogenic Media can be used for easy and economical detection of microbial contamination in cannabis. Alternatively,chromogenic media offers a convenient option to confirm the presence of viable microbial cells after a presumptive positive PCR result.

Detect with PCR

Confirm with chromogenic media

Detect with chromogenic media

Confirm with colony PCR

EnrichPerformance Specifications of Real-Time PCR Kits and Chromogenic MediaParameter

iQ-Check Real-Time PCRRAPID'Chromogenic Media

Assays available

Aspergillus*
E. coli O157:H7
Enterobacteriaceae
Listeria spp.
Listeria monocytogenes
Salmonella†
STEC VirX†STEC SerO II†

E. coliE. coli O157:H7
Enterobacteriaceae
Listeria
Salmonella
Staphylococcus

Principle

DNA-based detection of conserved genetic
targets using patented double-stranded probes

Chromogen-based colorimetric detection utilizing
bacterial enzymatic activity

Applications

Presumptive testing

Detection and/or confirmation post-PCR

Assay format

Qualitative real-time polymerase chain reaction,
ready-to-use reagents

Qualitative and quantitative culture media, readyto use and dehydrated

Time to results

2–2.5 hr post-enrichment

24 hr post-enrichment

Result interpretation

Automated using software

Visual interpretation of colored colonies

Reagent shelf life

2 years

3 months (ready to use)
3 years (dehydrated)

Cannabis and hemp flower, solvent- andnonsolvent-based extracts, gummies,chocolate, concentrates

As part of the AOAC-approved iQ-CheckSalmonella spp. method, RAPID'Salmonella
was evaluated as an alternative method forthe detection and confirmation of Salmonella
in cannabis and hemp flower, gummies,
chocolate, and concentrates†RESEARCH
INSTITUTE AOAC-approved
cannabis matrices* Aspergillus speciation kits available.
† Approved by AOAC Performance Tested Methods program for cannabis matrices.

BIO-RAD LABORATORIES, INC. | 3

Ordering Information
Catalog #DescriptioniQ-Check Kits
12010806
357811412003068
357812435781133578123
12013174
3578139

iQ-Check Aspergillus Kit, 96 reactions
iQ-Check E.coli O157:H7 Kit, 96 reactions
iQ-Check Enterobacteriaceae Kit, 96 reactions
iQ-Check Listeria monocytogenes II Kit, 96 reactions
iQ-Check Listeria spp. Kit, 96 reactions
iQ-Check Salmonella II Kit, 96 reactions
iQ-Check STEC SerO II Kit, 32 reactions
iQ-Check STEC VirX Kit, 96 reactions

iQ-Design Assays
12015337
iQ-Design Assay Aspergillus flavus, 100 reactions
12015260
iQ-Design Assay Aspergillus fumigatus, 100 reactions
12015336
iQ-Design Assay Aspergillus niger, 100 reactions
12018492
iQ-Design Assay Aspergillus terreus, 100 reactions
12015330
iQ-Design Assay Aspergillus Identification Positive Control
12018493
iQ-Design Assay Aspergillus + terreus Positive Control
Supplemental Reagents
3594970
iQ-Check Free DNA Removal Solution, 1 vial lyophilized
iQ-Check Free DNA Removal Reagent (to make 10 ml) and1 vial of iQ-Check 10x Activation Buffer (9 ml)
3578138
iQ-Check Lysis II Reagent
3578136
iQ-Check Lysis Beads
12012383
iQ-Check Purification Reagent, 1 vial to make 25 ml for 96 tests
Media for Sample Enrichment
3564684
Buffered Peptone Water Plus, dehydrated, 500 g
3554179
Buffered Peptone Water Plus, 225 ml x 6 bottles
3555795
Buffered Peptone Water Plus, 3 L x 4 bags
3555790
Buffered Peptone Water Plus, 5 L x 2 bags
12013259
Buffered Peptone Water Standard, dehydrated, 500 g
12013258
Buffered Peptone Water Standard, dehydrated, 5 kg
12013260
Buffered Peptone Water Standard, 5 L x 2 bags
3555703
Listeria Special Broth (LSB), 225 ml x 6 bottles3564703
Listeria Special Broth (LSB), dehydrated, 500 g
3564753
Listeria Special Broth (LSB), dehydrated, 5 kg
12017463
Listeria Special Broth II (LSB II), 225 ml x 6 bottles
12017388
Listeria Special Broth II (LSB II), dehydrated, 500 g
12017378
Listeria Special Broth II (LSB II), dehydrated, 5 kg
Chromogenic Media for Confirmation
3564748
RAPID'E.coli O157:H7 Agar, dehydrated, 100 g
3555299
RAPID'E.coli 2 Agar, ready to use, 100 ml x 6 bottles
3554012RAPID'Enterobacteriaceae Agar, ready to use, 200 ml x 6 bottles
3564744
RAPID'Listeria spp. Agar, dehydrated, 500 g
3563694
RAPID'L.mono Agar, 90 mm x 20 plates
3563963
RAPID'Salmonella Agar, 90 mm x 120 plates
3563960
RAPID'Staph Agar, ready to use, 90 mm x 20 plates
Instruments and Software
17007991
CFX Opus Deepwell Real-Time PCR System, 96 wells
3600037
CFX96 Touch Deep Well Real-Time PCR System, 96 wells
3594911
iQ-Check Prep System
3593893
CFX Manager Software, Industrial Diagnostic Edition (IDE)

Visit our website for more information and to see how our products
can be incorporated into cannabis testing workflows:
iQ-Check Real-Time PCR Kits — bio-rad.com/iQCheck
RAPID'Chromogenic Media — bio-rad.com/RapidMedia
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. IQ-CHECK is a trademark of Bio-Rad
Europe GmbH in certain jurisdictions. All trademarks used herein are the property of theirrespective owner. © 2024 Bio-Rad Laboratories, Inc.

“We use Bio-Rad’s iQ-Check Kits for their simplicity, ease ofuse, and scalability. The workflows are easily adaptable, which
is very important to our lab’s dynamic throughput, where wecould be testing one to a hundred samples a day. We also see
Bio-Rad as a partner in innovation, as their sales and technicalsupport teams work hand-in-hand with our lab to ensure ourmethods generate the highest quality data in the industry.”
NIKHIL KUMARCANALYSIS LABORATORIES“In a high-throughput environment like a cannabis testinglaboratory, having reliable and consistent automation isinvaluable. Partnering with Bio-Rad and utilizing theiQ-Check Prep automated platform has allowed us tovirtually eliminate operator error from our qPCR preparation.
With the iQ-Check Prep we are able to train even the newest
laboratory technicians to execute qPCR at an expert level
over the course of a few days.”

MICHAEL ESPOSITOMCR LABS“Choosing Bio-Rad is choosing reliability. Bio-Rad’s
iQ-Check Kits offer consistency, an intuitive workflow, andstraightforward data analysis to meet our high-throughput
needs as one of the largest cannabis testing labs in thecountry. What truly distinguishes Bio-Rad as our preferredpartner is the ongoing support from their knowledgeable
technical team and dedicated account managers.”
MAXWELL FAILINGAPOLLO LABS

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