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immunofluorescence detection of Listeria
monocytogenes using a monoclonal antibody. A
monoclonal antibody directed against Listeria
monocytogenes was produced by fusion of SP2/0 mouse
myeloma cells with splenocytes from mice immunisedwith Listeria-infected murine spleen cells. This antibody,designated MHM11, had a different fine specificity thanother antibodies that were previously raised against thisorganism. MHM11 reacted with extracellular,
subterminal and intracellular Listeria, with organisms ineither the exponential or stationary phase of growth.
When used in an indirect immunofluorescence assay(IIF) in combination with an FITC-conjugated secondantibody, the antibody reacted specifically with thecytoplasmic phase of Listeria infection of macrophages
at a MOI of 10. The antibody was used successfully todetect L. monocytogenes in non-freeze-fixed or freshlymixed sample preparations. The sensitivity of themethod was tested using cells cultured on BHI agar andcolonies from brain-heart infusion agar that had beenplated on BHI agar for 5, 7 and 11 h at 37 degrees C. In

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each of the three incubation periods examined,fluorescent colonies were clearly detected using the IIF
assay, while only weakly fluorescent colonies wereobtained with a conventional standard tube agglutination
test (MAT). When the sensitivity of the IIF assay wascompared with the conventional tube MAT, using thesame numbers of colonies from liquid culture inoculated
onto agar, the former was superior in detecting small
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page 3 / 3Copper Point Of Sale 142 UNLATTEED On Hotfile