Lonza Rockland, Inc.
www.lonza.com
scientific.support@lonza.com
Scientific Support: 800-521-0390
Document # 00525283-0911-01
Rockland, ME 04841 USA

ProSieve™ EX Running Buffer
Instructions for use
Introduction
Notes
ProSieve™ EX Running Buffer, a novel formulationof the classic Laemmli buffer, is a proprietary
solution to drastically increase the separation speed
without excessive heat generation affecting the gel.
The ProSieve™ EX Running Buffer is a direct
replacement for tris-glycine SDS running buffer.
Compatible with gels such as PAGEr™ Gold andother commercial precast or hand cast gels intended
to be used in a tris-glycine or Laemmli system.

Contents
Supplied as a 10X concentrate
Store at room temperature for up to 1 year from thedate of manufacture•

For best results use enough buffer in thechamber such that the outer chamber fills to justbelow the wells to help with cooling. Be sure tomonitor your run as the expected run time will bemuch shorter than that of standard TGS buffer.

•Buffer contains SDS and is designed for
denaturing gel electrophoresis. This buffer is notsuitable for running in a native environment.

•Follow with the ProSieve™ EX Transfer Buffer
for continued time savings into Western blot.

•For any particular gel concentration, theProSieve™ EX Running Buffer’s optimal size
range may shift slightly to favor smaller sizes
compared to gels run using standard tris-glycine
buffer.

Gel*
Size range forSize range forconcentration standard tris-glycine ProSieve™ EX
7.50%
50-325 kDa
20-250 kDa
10%30-180 kDa
15-140 kDa
12%
20-140 kDa
10-80 kDa
15%
12-100 kDa
5-70 kDa
4-12%
35-350 kDa
18-300 kDa
4-20%
13-300 kDa
10-225 kDa
8-16%
18-200 kDa
12-150 kDa
10-20%
10-120 kDa
5-100 kDa

ProtocolPrepare a 1X running buffer solution by diluting thestock buffer (10X solution) with distilled water.
Note: When using with PAGEr™ EX Gels use 0.67X
final concentration diluting the 10X stock 15-fold withdeionized water. (i.e. 67 ml 10X stock brought
to1000 ml with deionized water) and run according
to PAGEr™ EX Gel protocol.
Run gel under your normal conditions, between 50
and 250 V*Optimal size ranges based on PAGEr™ Goldtris-glycine precast gels, other commercial precast or
home-brew gels may have slightly different ranges.

Buffer concentration can be adjusted for faster
results:

Product Safety

MethodStandard

Bufferconc.
1XRunningvoltage200-250 V

For details regarding product safety, see material
safety data sheet (MSDS); call +1 (800) 638-8174
for extra copies of the MSDS. Emergency after
hours, call collect +1 (303) 595-9048.

Average running
time for minigel
18 – 35 min**Faster run times can be achieved under certain
conditions. Contact scientific.support@lonza.com for
more information.
All trademarks herein are marks of Lonza Groupor its subsidiaries.

1Ordering Information:
Catalog #00200307
00200308

Description
ProSieve™ EX Running Buffer
ProSieve™ EX Running Buffer

Size
1L4L

Related Products:
PAGEr™ Gold Tris-glycine Precast Gels
PAGEr™ Minigel Chamber and Blot Module Kit
ProSieve™ EX Transfer Buffer
ProSieve™ ProTrack™ Dual Color Protein Loading Buffer
ProSieve™ Blue Protein Staining Solution
ProSieve™ Protein Markers (stained and unstained)
AccuGENE™ Molecular Biology Grade Water

For research use only.
Not for use in diagnostic procedures.

Buffer technology licensed from DGel Electrosystem,
patents pending
2014 Lonza Rockland, Inc.
All rights reserved.

All trademarks herein are marks of Lonza Groupor its subsidiaries.

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