Main publications
1. T. Yanagida, M. Nakase, K. Nishiyama and F. Oosawa: Direct observation of motion of single F-actin
filaments in the presence of myosin

Nature. 307, 58-60 (1984)

2. T. Yanagida, T. Arata and F. Oosawa: Sliding distance of actin filament induced by a myosin crossbridge
during one ATP hydrolysis cycle.

Nature. 316, 366-369 (1985)

3. Y. Harada, A. Noguchi, A. Kishino and T. Yanagida: Sliding movement of single actin filaments on
one-headed myosin filaments.

Nature. 326, 805-808 (1987)

4. A. Kishino and T. Yanagida: Force measurements by micromanipulation of a single actin filament by glass
needles.

Nature. 334, 74-76 (1988)

5. A. Ishijima, T. Doi, K. Sakurada and T. Yanagida: Sub-piconewton force fluctuations of actomyosin in vitro.
Nature. 352, 301-306 (1991)
6. T. Funatsu, Y. Harada, M. Tokunaga, K. Saito and T. Yanagida: Imaging of single fluorescent molecules andindividual ATP turnovers by single myosin molecules in aqueous solution.	  Nature. 374, 555-559 (1995)
7. R. D. Vale, T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada and T. Yanagida: Direct observation of single
kinesin molecules moving along

Nature. 380, 451-453 (1996)

8. A. Ishijima, H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka and T. Yanagida: Simultaneous
observation of individual ATPase and mechanical events by a single myosin molecule during interaction withactin.

Cell. 92, 161-171 (1998)

9. C. Shingyoji, H. Higuchi, M. Yoshimura, E. Katayama and T. Yanagida: Dynein arms are oscillating force
generators.

Nature. 393, 711-714 (1998)

10.H. Yokota, K. Saito and T. Yanagida: Single molecule imaging of fluorescently-labeled proteins on metal
by surface plasmons in aqueous solution.

Phys. Rev. Letter. 80(20), 4606-4609 (1998)

11. K. Kitamura, M. Tokunaga, A. H. Iwane and T. Yanagida: A single myosin head moves along an actin
filament with regular steps of ~5.3nm.

Nature. 397, 129-134 (1999)

12. Y. Sambongi , Y. Iko, M. Tanabe, H. Omote, A. Iwamoto-Kihara, I. Ueda, T. Yanagida, Y. Wada and M.
Futai: Mechanical Rotation of the c Subunit Oligomer in the ATP Synthase (FoF1): Direct Observation.
Science. 286, 1722-1724 (1999)
13. Y. Harada, T. Funatsu, K. Murakami, Y. Nonoyama, A. Ishihama, T. Yanagida,of RNA polymerase-DNA interactions in real time.

Single molecule imaging

Biophys. J., 76, 709-715 (1999)

14. Y. Sako, S. Minoguchi and T. Yanagida: Single-molecule imaging of EGFR signaling on the surface ofliving cells.

Nature Cell Biology 2, 168-172 (2000)

15. M. Ueda, Y. Sako, T. Tanaka, P. Devreotes and T. Yanagida: Single molecule analysis of chemotactic
signaling in Dictyostelium cells.

Science 294, 864-867 (2001)

16. M. Nishiyama, E. Muto, Y. Inoue, T. Yanagida, H. Higuchi, Substeps within the 8-nm step of the ATPase
cycle of single kinesin molecules,

Nature Cell Biology, 3, 425-428 (2001)

17. H. Tanaka, K. Homma, A. H. Iwane, E. Katayama, R. Ikebe, J. Saito, T. Yanagida and M. Ikebe: The motor
domain determines the large step of myosin-V.

Nature 415, 192-195 (2002)

18. M. Nishiyama, H. Higuchi and T. Yanagida: Chemomechanical coupling of the ATPase cycle to the1

forward and backward movements of single kinesin molecules.
Nature Cell Biology. 4, 790-797 (2002).
19. T. Murata, N. Matsui, S. Miyauchi, Y. Kakita, T. Yanagida, _Discrete stochastic process underlying
perceptual rivalry.

Neuroreport, 14, 1347-1352 (2003)

20. K. Kitamura, M. Tokunaga, S. Esaki, A. H. Iwane and T. Yanagida. Mechanism of muscle contraction
based on stochastic properties of single actomyosin motors observed in vitro.

Biophysics, 1, 1- 19 (2005)

21. Y. Taniguchi, M. Nishiyama, Y. Ishii, T. Yanagida. Entropy rectifies the Brownian steps of kinesin,
Nature Chem. Biol., 1, 346-351 (2005)
22. J. Kozuka, H. Yokota, Y. Arai, Y. Ishii, T. Yanagida, _Dynamic polymorphism of single actin molecules inthe actin filament,

Nature Chem. Biology, 2, 83-86 (2006)

23. Y. Komori, A. Iwane and T.Yanagida: Myosin-V makes two Brownian 90o rotations per 36 nm step.
Nature Str. Mol. Biol. 14（10）:968-973 (2007)
24. M. Nishikawa, H. Takagi, T. Shibata, A. H. Iwane, T. Yanagida, _Fluctuation Analysis of
Mechanochemical Coupling Depending on the Type of Biomolecular Motors,
Phys. Rev. Lett., 101(12), 128103 (2008)
25. M. Iwaki, A. H. Iwane, T. Shimokawa, R. Cooke, T. Yanagida. Brownian search-and-catch mechanism formyosin-VI steps.

Nature Chem. Biol., 5(6), 403-405 (2009)

26. Y. Tsuchiya, T. Komori, M. Hirano, T. Shiraki, A. Kakugo, T. Ide, J, -P. Gong, S. Yamada, T. Yanagida, S.
Shinkai,

A Polysaccharide-Based Container Transportation System Powered by Molecular Motors,Angew. Chem. Int. Ed. , 49, 724-727 (2010)27.	  S.	  Nishikawa,	  I.	  Arimoto,	  K.	  Ikezaki,	  M.	  Sugawa,	  H.	  Ueno,	  T.	  Komori,	  A.	  H	  Iwane,	  T.	  Yanagida.
Switch	  between	  large	  hand-­‐over-­‐hand	  and	  small	  inchworm-­‐like	  steps	  in	  myosin	  VI	  	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   	   Cell	  in	  press	  (2010)	  28. T. Fujii, A. H. Iwane, T. Yanagida, K. Namba. Direct visualization of secondary structures ofF-actin by electron cryomicroscopy.

Nature in press (2010)

Review and Book
1.

T. Yanagida, K. Kitamura, H. Tanaka, A. H. Iwane and S. Esaki: Single molecule analysis of theactomyosin motor.
Current Opinion in Cell Biology. 12, 20-25 (2000)
2.

Y. Ishii and T. Yanagida: Single Molecule Detection in Life Science.
Single Molecules. 1, 5-14 (2000)

3.

Y. Sako and Yanagida, T. (2003) :Single-molecule visualization in cell biology.
Nature Rev. Mol. Cell Biol. 4, ss1-ss6 (2003)

4.

A. Ishijima and T. Yanagida: Single Molecule Nano-Bioscience.
Trends in Biochemical Sciences.26, 438-444 (2001) SS1-5 (2003).

5.

Y. Ishii, Kitamura K, Tanaka H, Yanagida T: Molecular motors and single-molecule enzymology.
Methods in Enzymol. 361:228-245 (2003).

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6.

T. Yanagida and Y. Ishii (ed) Single Molecule Dynamics in Life ScienceBook(Wiley-VCH)(2009)

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