Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18105-0807-7
Rockland, ME 04841 USA

SeaPlaque® GTG® Agarose
For reliable separation and recovery of DNA >1,000 bp.
Precautions
Always wear eye protection when dissolving agaroseand guard yourself and others against scalding
solutions. Refer to Material Safety Data Sheet for
additional safety and handling information.

Introduction
SeaPlaque® GTG® Agarose is a low melting
temperature agarose designed for the separation of DNA
fragments between 100 bp to 23,000 bp. SeaPlaque®
GTG® Agarose is ideally suited for direct enzymatic
manipulation of nucleic acids in remelted agarose.
SeaPlaque® GTG® Agarose is tested and certified forligation-transformation and restriction digestion using
remelted agarose protocols.
Analytical Specifications
Gelling temperature (1.5%)
Melting temperature (1.5%)
Gel Strength (1%)

Microwave Instructions for Agarose Preparation
1. Choose a beaker that is 2-4 times the volume of thesolution.
2. Add room temperature 1X or 0.5X electrophoresis
buffer and a stir bar to the beaker.
3. Slowly sprinkle in the agarose powder while thesolution is rapidly stirred.
4. Remove the stir bar if not Teflon® coated.
5. Weigh the beaker and solution before heating.
6. Cover the beaker with plastic wrap.
7. Pierce a small hole in the plastic wrap for ventilation.
8. Heat the beaker in the microwave oven on Highpower until bubbles appear.
9. Remove the beaker from the microwave oven.
Caution: Any microwaved solution may
become superheated and foam over whenagitated.
10. GENTLY swirl the beaker to resuspend any settled
powder and gel pieces.
11. Reheat the beaker on HIGH power until the solution
comes to a boil.
12. Hold at boiling point for 1 minute or until all of theparticles are dissolved.
13. Remove the beaker from the microwave oven.
14. GENTLY swirl the beaker to thoroughly mix theagarose solution.
15. After dissolution, add sufficient hot distilled water toobtain the initial weight.
16. Mix thoroughly.
17. Cool the solution to 50°C-60°C prior to casting.

26°C-30°C<65°C>200 g/cm²

Applications• Electrophoresis of DNA fragments >1,000 bp• Separation and recovery of megabase-sized DNAfragments with ß-Agarase• Direct enzymatic manipulation of DNA in remelted
agarose
Suggested Agarose Concentrations
Size Range

Final Agarose Concentration (%)(Base Pairs)

1X TAE Buffer

1X TBE Buffer

500-25,000
300-20,000
200-12,000
150-6,000
100-3,000
50-2,000

0.75
1.00
1.25
1.50
1.75
2.00

0.70
0.85
1.00
1.25
1.50
1.75

Dye Mobility Table
Migration of double-stranded DNA in relation toBromophenol Blue (BPB) and Xylene Cyanol (XC) in
SeaPlaque® GTG® Agarose gels.
1X TAE Buffer%

1X TBE Buffer

XC

BPB

AgaroseXCBPB

11,700
4,000
2,300
1,500
1,000
700
550
320

1,020
500
350
200
150
100
6030

0.50
0.75
1.00
1.25
1.50
1.75
2.00
2.50

6,100
2,850
1,700
1,000
700
500
400
250

400
280
180
100
7050
30
101Hot Plate Instructions for Agarose Preparation
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.

Choose a beaker that is 2-4 times the volume ofthe solution.
Add room temperature electrophoresis buffer
and a stir bar to the beaker.
Slowly sprinkle the agarose powder while thesolution is rapidly stirred.
Weigh the beaker and solution before heating.
Cover the beaker with plastic wrap.
Pierce a small hole in the plastic wrap forventilation.
Bring the solution to a boil while stirring.
Maintain gentle boiling until all the agarose isdissolved (approximately 10 minutes).
Add sufficient hot distilled water to obtain theinitial weight.
Mix thoroughly.
Cool the solution to 50°C-60°C prior to casting.

Ordering Information:
Catalog No.
50111
50110

Size
25 g
125 g

For more information on SeaPlaque® GTG® Agarose,contact Technical Service at (800) 521-0390 or visit ourwebsite at www.Lonza.com.

Related Products:
DNA Ladders
DNA MarkersGelStar® Nucleic Acid Gel Stain
AccuGENE® TBE and TAE Buffers
AccuGENE® Molecular Biology Buffers
ß-Agarase
For Laboratory Use.

GelStar is a trademark of FMC Corporation. Teflon is a trademark ofE.I. duPont de Nemours and Co. All other trademarks herein aremarks of the Lonza Group or its affiliates. GelStar is covered by U.S.
patent 5,436,134. Other U.S.and foreign patents pending.
© 2007 Lonza Rockland, Inc.
All rights reserved.