Anti-Calreticulin-3 / CALR3 / Calsperin antibody, rabbit serum
Product code73-022

Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.1% sodium azidePurity

Rabbit antiserum

ImmunogenSynthetic peptide corresponding to C-terminal region of mouse CALR3,CMGKFHRHNHLSRFHRQGEL.

IsotypeRabbit IgG

Reactivity

Mouse.
Not tested with other species.

Special notes

N/A

Application

1. Western blotting (1/1,000 dilution))
2. Immunoprecipitation (1/100 dilution)
3. Immunohistochemistry (1/100 dilution)
Other applications have not been tested.

Background

CALR3 capacity for calcium-binding may be absent or much lower than that ofCALR. During spermatogenesis, may act as a lectin-independent chaperone for
specific client proteins such as ADAM3. Required for sperm fertility.
Molecular mass: 44,232 with 380 amino acids.

Data Link

uniprot/Q9D9Q6 mouse, Gene ID 73316 mouse

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 73-022 Anti-Calreticulin-3 / CALR3 / Calsperin antibody, rabbit serum

Fig. 1. Testeis specific expression of Calreticulon-3 as examined in various tissues by western blottingwith anti-CALR3 antibody.
The various tissues were excised and homogenized in lysis buffer containing 1% TritonX100 and thenplaced on ice for 1 h. These extracts were centrifuged, and the supernatants were collected andanalyzed by western blotting with anti-CALR3 antibody at 1/1,000 dilution.
1.Brain. 2. Lung. 3. Heart. 4. Thymus. 5. Liver. 6. Spleen. 7. Kidney. 8. Muscle. 9. Ovary. 10. Testis.
11. SpermFig.2. Identification of Calreticulon-3 protein by western blotting with anti-CALR3 antibody.
Embryonic fibroblast cells prepared from Calr3 -/- mouse were transfected with a plasmid expressing

Calr3. The cell lysate was analyzed by western blotting with anti-CALR3 antibody at 1/1,000 dilution.
1. Mock-infected cell lysate as a negative control.
2. Cell lysate transfected with a plasmid expressing Calr3.

2 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig.3. Analysis of Calrecticulon-3 protein in the extracts of mouse testis and sperm by western
blotting with anti-Calrecticulon-3 antibody.
Proteins in the extracts (10 µg protein) were separated on SDS-PAGE (10-20% gradient gel), electroblotted to PVDF membrane and reacted with anti-Calrecticulon-3 antibody at 1/1,000 dilution. As thesecond antibody, anti-rabbit IgG antibody conjugated with HRP (Abcam:ab97051) was used at
1/10,000 dilution. The numbers on the left are positions of protein size markers in kDa.

Fig.4. Immunoprecipitation of Calreticulon-3 protein with anti-CALR3 antibody.
Lysates of wild-type mouse testis were immunoprecipitated with anti-CALR3 antibody and theprecipitates were analyzed by western blotting with the same antibody.
1. Input testis lysate
2. Precipitated with preimmune serum
3. Precipitated with anti-CALR3 antibody

3 / 4BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig.5

Immunofluorescence staining of a testicular section with anti-CALR3 antibody.

Sequential sections were stained with hematoxylin and eosin (upper panel). CALR3 was detected inelongating spermatids (lower panel). Testis was collected from adult mouse and fixed in 4%
paraformaldehyde/PBS overnight at 4 °C, cryopreserved in graded 10–30% sucrose, and embedded.
Frozen sections (8 μm) were mounted on aminopropyltriethoxysilane-coated glass slides. Primaryantibody was used at 1/100 and as secondary antibody, Alexa Fluor 488 conjugated goat anti-rabbit
IgG was used. Scale bar is 200 μm.
Reference: This antibody was described and used in the following publication.
1. Ikawa M. et al (2011) Calsperin is a testis-specific chaperone required for sperm fertility. J Biol
Chem.18:5639-46. pubmed/21131354 WB, IP, IHC. Free article.

4 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/