Lonza Rockland, Inc.
www.lonza.com
Scientific.suppport@lonza.com
Scientific Support: 800-521-0390
Customer Service: 800-638-8174
Document # 18914-0111-06
Rockland, ME 04841 USA

I.D.NA™ Agarose
A high gel strength fast running agarose for superior resolution of DNA fragments under DNA identity testing conditions

Microwave Procedure

Certificate of Performance

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2.

Weigh out agarose.
Tare beaker or wide-mouthed flask containing a magnetic
stir bar.
3. Add room temperature buffer (or distilled water) to thevessel. Be sure to choose a vessel that is 2-4 times thevolume of solution.
4. Slowly sprinkle the agarose powder into the buffer withconstant stirring.
5. Using a glass rod, break up and disperse any agarose
clumps and scrape down any powder adhered to the wallsof the flask. Stir for a minimum of 3 minutes. Remove
magnetic stir bar, if used.
6. Cover the flask with plastic wrap and pierce a small hole infrom the plastic as a steam vent.
7. Weigh the flask and its contents.
8. Heat covered flask in the microwave oven on high power
setting (100%) for 2 minutes.
9. Remove container from oven and gently swirl toresuspend settled powder and gel pieces.
10. Reheat on high for 2 minutes, until the solution comes to aboil. Hold at boiling point for 1 minute, until all particles
are dissolved.
11. Remove from microwave. Gently swirl to thoroughly mix
the agarose solution.
12. Remove the plastic wrap from the flask and adjust theweight of the solution to the desired final weight with hot
distilled water.
13. Mix and cool to 60°C before casting.

Chemical and Physical Specifications:
1.
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Gelling temp., 1.5%
Moisture:Sulfate:Gel Strength, (1%):
EEO (-mr):RNase:
DNase:DNA Binding:√√√√√√√√

36±1.5°C≤10%≤0.15%≥1300 g/cm²≤0.10
None detected
None detected
None detected™

DNA Markers, electrophoresed on a 1% I.D.NA Agarose gel,are finely resolved without smearing and quantitativelyretained.

References:
Budowle, B., Adams, D.E., and Allen, R.C.,“Fragment Length Polymorphisms for Forensic ScienceApplications,”
Methods in Nucleic Acid Research, 181-202, Edited byKaram, J.D., Chao, L, and Warr,G.W. Baton Rouge, Florida:
CRC Press, Inc., 1991. Budowle, B. and Baechtel, S.S.,“Modifications to improve RFLP Typing”,
Applied & Theoretical Electrophoresis, 1, 181-187.

Dissolving I.D.NA™ Agarose
Boiling Water Bath Procedure

Warranty

1.
2.
3.

Weigh out agarose.
Tare beaker containing a magnetic stir bar.
Add a measured volume of buffer (or distilled water) at
room temperature to the beaker.
4. Slowly sprinkle the agarose into the room temperaturebuffer with continuous stirring.
5. Allow the agarose particles to disperse completely.
6. Weigh the beaker and its contents (buffer, agarose, andstir bar.)
7. Cover the beaker with plastic wrap.
8. Punch a hole to the walls prevent pressure buildup.
9. Heat the solution to boiling while stirring, then maintaingentle boiling for 10 minutes.
10. Remove the plastic wrap from the beaker and adjust theweight of the solution to the desired final weight with hot
distilled water.
11. Mix and cool to 60°C before casting the gel.

Because of the numerous factors affecting results, Lonza
Agarose is sold with the understanding that purchasers will
make their own tests to determine the suitability of this productfor their particular purposes. The several uses suggested byLonza, are presented only to assist our customers in exploring
possible applications for this product. All information and data
presented are believed to be accurate and reliable but arepresented without the assumption of any liability by Lonza. All
products are sold subject to the standard terms and conditions
and limitations contained on our order acknowledgment.

Patent Position
Lonza does not warrant against infringements of patents ofthird parties by reason of any uses made of the product in
combination with other material or in the operation of anyprocess, and purchasers assume all risks of patent
infringement by reason of any such use, combination, oroperation.

1Product Safety
There are no demonstrated acute hazards for agaroses. A
Material Safety Data Sheet (MSDS) is available upon request;
call (800) 638-8174 or go to our website www.Lonza.com.
Emergency number after hours, call collect (202) 483-7616.

Ordering Information
Catalog No

Description

Size50170

I.D.NA Agarose

125 g™

Related Products
AccuGENE™ TBE Buffer

For Laboratory Use.
Derived from Agar.
All trademarks herein are marks of the Lonza Group or its affiliates.
© 2010 Lonza Rockland, Inc.
All rights reserved.

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