Anti-DDX3X antibody, rabbit serum
Product code

70-450Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

ImmunogenPurified Human DDX3X fused with GST

IsotypeRabbit IgG

Reactivity

DDX3X of human monkey and rodents. Not tested with other species.

Special notes

N/A

Application

1. Western blotting (1/1,000~ 1/5,000)
2. Immunofluorescence staining (1/500)
3. Immunohistochemical staining (1/200)

Background

DDX3X (662aa, 73.2 kDa) is a member of DEAD box proteins, characterized by
the conserved motif Asp-Glu-Ala-Asp (DEAD) which are putative RNAhelicases. They are implicated in a number of cellular processes involving
alteration of RNA secondary structure such as translation initiation, nuclear
and mitochondrial splicing, and ribosome and spliceosome assembly. Based on
their distribution patterns, some members of this family are believed to beinvolved in embryogenesis, spermatogenesis, and cellular growth and division.
This gene encodes a DEAD box protein, which interacts specifically withhepatitis C virus core protein resulting a change in intracellular location. Thisgene has a homolog located in the nonrecombining region of the Y chromosome.
The protein sequence is 91% identical between this gene and the Y-linkedhomolog. Alternative splicing results in multiple transcript variants.

Data Link

UniProtKB

O00571 (DDX3X_HUMAN) GeneCards:DDX3X Gene

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-450 Anti-DDX3X antibody, rabbit serum

Fig.1 Western blot analysis of endogenous DDX3X in HeLa whole cell lysate
HeLa cells (10µg) with anti- DDX3X antibody at 1/1,000 dilution and as the second antibody, HRPconjugated goat anti-rabbit IgG was used at 1/20,000 dilution.

Fig.2 Immunostaing of HeLa cells with anti-DDX3X antibody. Paraformaldehyde-fixed HeLa cells
were stained with anti-DDX3X antibody at 1/500 dilution. DDX3X is localized in cytoplasm in theabsence of treatment, but once treated with LMB, a nuclear translocation inhibitor, it is localized not
only in cytoplasm but also in nuclei.

Fig.3 Immunostaining of Hela cells with anti-DDX3X antibody.
Hela cells were stained with anti-DDX3X antibody (red) at 1/500 dilution and as the secondary
antibody, Alexa Fluor 568-conjugated goat anti-rabbit antibody at 2 µg/ml. The nuclei were stainedwith Hoechst (blue).
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Fig.4 Immunohistochemical staining of DDX3X in mouse testis tissue.
Frozen section was stained with anti-DDX3X antibody at 1/200 dilution and secondarily labeled with
Cys-conjugated anti-rabbit IgG. Arrows indicate Leydig cells.
Reference : This product was described and used in the following publications.
1 .Sekiguchi T et al Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster
temperature-sensitive ET24 mutant cell line with a DDX3X mutation. Exp. Cell. Res. 300 : 213-222(2004) PMID: 15383328. IF, IHC-F (hu, ms, ham)
2. Sekiguchi T. et al. Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin
B/cdc2 in vitro. Biochem Biophys Res Commun. 2007 May 11;356(3):668-73. PMID: 17379183 WB(hu, ham)

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