Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-638-8174
Customer Service: 800-638-3174
Document # 18155-1007-07
Rockland, ME 04841 USA

Latitude® Precast Gels
Precast agarose midigels that save you time.
Introduction
Latitude® Precast Agarose Midigels are manufactured
from high quality, Lonza molecular biology grade
SeaKem® and NuSieve® Agarose. The gels fit moststandard electrophoresis chambers and are stable atroom temperature for 6-12 months depending upon
formulation. For best results use the Latitude® MidigelChamber.

6.

7.
Read Before Following the Protocol•••••

Caution: These gels may contain 0.5 μg/ml
ethidium bromide, which is a powerful mutagen.
Gloves, lab coat and safety glasses should beworn when opening pouches and handling gelsand any solutions containing ethidium bromide.
Refer to MSDS for safety precautions.
Decontamination of ethidium bromide isdescribed in Sambrook et al., Molecular Cloning,
A Laboratory Manual (1989), 6.16-6.17.
Avoid skin and eye exposure to UV light.
Do not drop Latitude® Gels as they can crack atthe wells.
For best band resolution, add 0.5 μg/ml ethidium
bromide to the running buffer .
Latitude® Precast Agarose Midigels are designed
to be run in the tray using the TruBand® Anchor
for convenience, speed and optimal results.

8.
9.

the top of the anchor. It is better to have the buffer
level slightly below the top of the anchor than evenwith the top of the anchor.
Load DNA samples (10 μl-12 μl per well for optimalresolution) following standard procedures. The
midigels are configured for alternate-well loading
using 8 or 12 well multichannel pipettes.
Shift the TruBand® Anchor so that it is centered overthe gel and all wells are completely covered before
beginning electrophoresis. See diagram below.
Electrophorese the gel at 5–10 V/cm interelectrodedistance.
Remove the anchor, and remove the gel from thechamber. Carefully remove the gel from the tray,
place on the light box and photograph as usual. The
tray is not UV transparent and gels must be
removed from the tray for imaging with a UVtransilluminator. If smaller DNA fragments are notdetected, briefly stain the gel (10 to 15 minutes) in
0.5 μg/ml ethidium bromide.
LOAD®

With TruBandProtocol
1.
Cut open the gel pouch on one end and remove thegel. Exercise caution when removing the gel as
the pouch contains a small amount of liquid that
may contain ethidium bromide.
2.
Leave the gel in the tray and place directly on thechamber platform.
3.
Cover the gel with the appropriate buffer (1X TAE or
1X TBE) to a depth of 2 mm-3 mm over the gel.
4.
Place the appropriate size TruBand® Gel Anchor
over the tray so that the white gaskets fit snugly on
the flanges on the sides of the tray. Position theanchor so that the top and middle tiers of wells areexposed. See diagram right.
5.
Add buffer until the level is even with the top of theanchor. Very Important! Do not flood buffer overRUN®

AnchorWith TruBand Anchor

1Ordering Information
Gel Size: 10 x 15 cm 8 midigels per box
Catalog No.

Agarose Concentration

BufferRelated Products

DNA Size®

AccuGENE TAE, TBE and MOPS Buffer
DNA Markers
DNA Ladders
RNA Marker®
Latitude Midigel ChamberRange
1 x 20 well 1X TAE buffer + ethidium
bromide57200

1X

400 bp≥10 kb

TAE57201

100 bp≥3 kb®

57202

3.6% NuSieve Hi-Res

20 bp≥1 kb

2 x 20 well 1X TAE buffer + ethidium
bromide57210

1% SeaKem® LE Plus

1X

400 bp≥10 kb

TAE
®

57211

2% SeaKem LE Plus

57212

3.6% NuSieve Hi-Res

100 bp≥3 kb®

20 bp≥1 kb

6 x 18 well 1X TAE buffer + ethidium
bromide®
1% SeaKem LE Plus

57263

1X

400 bp≥10 kb

TAE
®

57265

2% SeaKem LE Plus

100 bp≥3 kb

®

57267

3.6% NuSieve Hi-Res

1 x 20 well 1X TBE buffer + ethidium
bromide®
1% SeaKem LE Plus

57220

1X

300 bp≥8 kb

TBE®

57221

2% SeaKem LE Plus

100 bp≥2 kb

®

57222

4% NuSieve 3:1 Plus

8 bp≥1 kb

2 x 20 well 1X TBE buffer + ethidium
bromide®
1% SeaKem LE Plus

57230

1X

300 bp≥8 kb

TBE
®

57231

2% SeaKem LE Plus

100 bp≥2 kb

®

57232

4% NuSieve 3:1 Plus

8 bp≥1 kb

6 x 18 well 1X TAE buffer + ethidium

For more information contact Technical Service at(800) 521-0390 or visit our website at www.Lonza.com

bromide®
1% SeaKem LE Plus

57264

1X

300 bp≥8 kbTBE®

57266

2% SeaKem LE Plus

100 bp≥2 kb

®

57268

4% NuSieve 3:1 Plus

8 bp≥1 kb®

Latitude Midigels Gel size: 10 x 15 cm gels
With 1X MOPS buffer for RNA analysis
Catalog No.

Gel Type

57238®

1.25% SeaKem Gold for RNA

57237

®

For Laboratory Use.

Description

1.25% SeaKem Gold for RNA

1 x 20 well

SeaKem is a trademark of FMC Corporation. All othertrademarks herein are marks of the Lonza Group or its
affiliates.

2 x 20 well®

TruBand Anchor for 10 x 15 cm gels
56989
56988

© 2007 Lonza Rockland, Inc.
All rights reserved.

For Standard chambers®

For Latitude Midigel Chamber2