Lonza Rockland, Inc.
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Document # 00522217-0911-02
Rockland, ME 04841 USA

ProSieveTM QuadColor™ protein marker, 4.6 kDa – 300 kDa
Instructions for use
Introduction

Contents

The ProSieve™ QuadColor™ protein marker is a
mixture of 12 recombinant, highly purified proteins
with molecular weights of 4.6, 10, 15, 25, 40, 55, 70,100, 140, 170, 250, and 300 kDa. The proteins areindividually prestained using four different dyes,
producing a brightly colored ladder with an easy-toremember pattern. The ProSieve™ QuadColor™
protein marker is ready-to-use: no heating, further
dilution or addition of a reducing agent is required
before use.

Cat. No. 00193837
ProSieve™ QuadColor™ protein marker, 2 x 250 µl(50 minigel applications @ 10 µl per well or 25 large
gel applications @ 20 µl per well)
Storage buffer
62.5 mM Tris-H3PO4 (pH 7.5), 1 mM EDTA,2% (w/v) SDS, 10 mM DTT, 1 mM NaN3 and
33% (v/v) glycerol.
Store at -20°C for 2 years or at 4°C for 3 months

ProSieve™ QuadColor™ protein marker is ideal forseveral applications.
•••Protocol

Monitoring of protein migration during SDSPAGE1.
Verifying Western transfer efficiency2-4.
Approximate sizing of proteins on SDSpolyacrylamide gels and Western blots.

1. Thaw the ladder at room temperature for a few
minutes to dissolve precipitated solids. Do notboil!
2. Mix gently, but thoroughly, to ensure that thesolution is homogeneous.
3. Load the following volumes of the marker on anSDS-polyacrylamide gel.
For GelFor BlotAnalysis Analysis
Minigel* 5-10 μl
5-10 μl
Large gel
20 μl10 μl

Typical band sizes*kDa
300
250
170
140

4. Use the same volumes for Western blotting.

10070* Optimal volume dependent on well size.

55
40NOTES

25

•

15
10

•

4.6
* Some band sizes may vary from lot to lot. Lot specificreference card is supplied with each marker.

••

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Low molecular weight proteins may migrate withthe dye front on low concentration gels (e.g.
7.5% & 10%).
Longer transfer times or higher transfer voltages
may be required for Western blotting of large(>100 kDa) proteins.
For precise protein molecular weight
determination use ProSieve™ unstained protein
markers.
Lot specific calculated apparent MW, kDa on 420% tris-glycine SDS-PAGEReferences
1. Laemmli, U.K. (1970). Cleavage of structuralproteins during the assembly of the head ofbacteriophage T4. Nature, 227: 680-685.
2. Burnette, W.N. (1981). "Western blotting":
electrophoretic transfer of proteins from sodium
dodecyl sulfate – polyacrylamide gels to
unmodified nitrocellulose and radiographic
detection with antibody and radioiodinatedprotein A. Anal. Biochem., 112 (2): 195-203.
3. Towbin, H., et al. (1979). Electrophoretic transferof proteins from polyacrylamide gels tonitrocellulose sheets: procedure and someapplications. PNAS, 76: 4350-4354.
4. Kurien, B.T. and Scofield, R.H. (2003). Protein
blotting: a review. J. Imm. Meth., 274: 1-15.
Product safety
For details regarding product safety, see material
safety data sheet (MSDS); call +1 (800) 638-8174
for extra copies of the MSDS. Emergency after
hours, call collect +1 (303) 595-9048.

Manufactured for Lonza Rockland, Inc.
For research use only.
Not for use in diagnostic procedures.
All trademarks herein are marks of the Lonza Group or its
subsidiaries.
2011 Lonza Rockland, Inc.
All rights reserved.

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