Anti-Influenza B Virus M1 antibody, mouse monoclonal (6A11)
Product code65-155Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoriagroup.

Isotype

Mouse IgG1κ

Reactivity

Reacts with M1 proteins of all Influenza B viruses so far tested (100 strains) as
examined by ICC staining, including lineage Yamagata strains; Mie/1/1993,
JohanesBurg/5/1999, Florida/4/2006 and lineage Victoria strains; Lee/1940,Gif/21/1973, Shangdong/7/1997, Malasia/2506/2004, Massachustts/2/2012
No cross reactivity with influenza A viruses.

Special notes

N/AApplication

1. Western blotting (1/500~1/1,000 dilution)
2. Immunofluorescent and Immunocytochemical staining(1/100~1/200dilution)
3. Immunoprecipitation (1/200 dilution)
4. ELISA (assay dependent)

Background

Matrix protein 1 (M1) plays critical roles in virus replication, from virus entry anduncoating to assembly and budding of the virus particle. M1 binding toribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interactionof viral NEP with M1-RNP is thought to promote nuclear export of the complex,
which is targeted to the virion assembly site at the apical plasma membrane inpolarized epithelial cells. Interactions with NA and HA may bring M1, a non-raftassociated protein, into lipid rafts. Forms a continuous shell on the inner side of thelipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2ion channel acidifies the internal virion core, inducing M1 dissociation from theRNP. M1-free RNPs are transported to the nucleus, where viral transcription andreplication can take place.
M1 of Influenza B virus consists of 248 amino acids with molecular mass of 27,415.

Data Link

UniProtKB A4D3F9 Influenza B virus Matrix 1 protein

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-155 Anti-Influenza B Virus M1 antibody, mouse monoclonal (6A11)

Fig. 1 Detection of M1 protein in the crude extracts of MDCK cells infected with various Influenza B
virus strains by using 6A11 monoclonal antibody.
1. B/Florida/4/2006
2. B/Lee/1940
3. B/Malasia/2506/2004
4. B/Massachusetts/2/2012
First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG
antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left.
MockInfected

Fig.2 Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus,
using anti-Influenza B virus M1 antibody (clone 6A11).
Anti-Influenza B Virus M1 antibody (clone 6A11) efficiently detected the viruses in the infected
MDCK cells with B/Florida/4/2006 virus. The cells were ﬁxed with 4% paraformaldehyde inphosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The antibody(6A11) was used at 1/100 ditlution and the bound antibody was visualized by a further reaction withan Alexa Fluor 488-conjugated secondary antibody (green).
Image on the left is a negative control, mock-infected MDCK cells.
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References: This antibody was described and used in the following reference.
1. Nakagawa N. et al. Rapid detection and identification of two lineages of influenza B strains withmonoclonal antibodies. J Virol Methods. 1999;79:113-2 ICC, IP

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