Lonza Rockland, Inc.
www.lonza.com
scientific.support@lonza.com
Scientific support: 800-521-0390
Customer service: 800-638-8174
Document # 18139-0911-07
Rockland, ME 04841 USA

Lonza SimplyLoad™ DNA ladders
Introduction

Contents

Lonza SimplyLoad™ DNA ladders are ready-to-load directly on
your gel to maximize your time. Each band has an ideal 50%
G-C content for rapid, precise sizing and sharp resolution. Ourladders are free from contaminating plasmid DNA so they haveminimal background.

SimplyLoad™ DNA ladders are supplied at ready-to-loadconcentrations in TE Buffer and 1X Ficoll® sample loading
buffer containing Bromophenol blue and Xylene cyanol FF.
500 µl total volume.

Description

Store at 2°C-8°C. Contact scientific support for shelf life
information

Storage50331 - SimplyLoad™ 20 bp DNA ladder: 40 ng/µl
25 fragments from 20 bp to 500 bp, in 20 bp increments.
The band at 200 bp stains brighter to aid identification.
50326 - SimplyLoad™ 20 bp ext range DNA ladder:
60 ng/µl 50 fragments from 20 bp to 1,000 bp, in 20 bpincrements. Bands at 200 bp and 500 bp stain brighter to aididentification.
50327 - SimplyLoad™ 100 bp DNA ladder: 32 ng/µl
10 fragments from 100 bp to 1,000 bp, in 100 bp increments.
50328 - SimplyLoad™ 100 bp Ext Range DNA ladder: 60 ng/µl
30 fragments from 100 bp to 3,000 bp, in 100 bp increments.
Bands at 1,000 bp and 3,000 bp stain brighter to aid identification.
50333 - SimplyLoad™ Tandem DNA ladder: 60 ng/µl
21 fragments from 100 bp to 12,000 bp, in 100 bp and 1,000bp increments. Bands at 1 kb and 5 kb stain brighter to aididentification.
50329 - SimplyLoad™ 500 bp DNA ladder: 60 ng/µl
16 fragments from 500 bp to 8,000 bp, in 500 bp increments.
The band at 5 kb stains brighter to aid identification.

Amounts to Load
Load 5 µl of SimplyLoad™ DNA ladder into a single lane.
For complete band resolution run marker the full length of thegel.

Use with Lonza MetaPhor™, NuSieve™ 3:1 andNuSieve™ GTG™ agarose

20 bp ladder

20 bp ext ladder

Use with Lonza SeaKem® LE, SeaKem®, GTG™
™
™
™
SeaPlaque and SeaPlaque , GTG agarose

100 bp ladderTandem ladder— 12 kb— 7 kb— 5 kb

— 1,000 bp— 500 bp
— 400 bp

— 500 bp
— 400 bp

— 300 bp

— 300 bp

— 200 bp

— 200 bp

— 140 bp

— 140 bp— 100 bp— 100 bp

— 60 bp— 60 bp

100 bp ext ladder— 1,000 bp— 800 bp
— 600 bp
— 400 bp

— 3 kb— 3 kb— 2 kb

— 2.5 kb
— 2 kb
— 1.5 kb

500 bp ladder— 8 kb— 5 kb
— 3.5 kb
— 2.5 kb
— 2 kb
— 1.5 kb

— 800 bp

— 1 kb— 800 bp

— 600 bp

— 600 bp

— 400 bp

— 400 bp

— 1 kb— 200 bp— 100 bp
— 20 bp— 20 bp

1— 200 bp— 100 bp

— 200 bp— 100 bp

— 1 kb— 500 bp

Ordering information
Cat No.
50331
50326
50327
50328
50333
50329

Description
SimplyLoad™ 20 bp DNA ladder
SimplyLoad™ 20 bp ext range DNA ladder
SimplyLoad™ 100 bp DNA ladder
SimplyLoad™ 100 bp ext range DNA ladder
SimplyLoad™ Tandem DNA ladder
SimplyLoad™ 500 bp DNA ladderSize
100 applications
100 applications
100 applications
100 applications
100 applications
100 applications

For more information contact scientific support at+1 (800) 521-0390 or visit our website atwww.lonza.com

SeaKem is a trademark of FMC Corporation. Ficoll is atrademark of GE Healthcare Bio-Sciences AB, Ltd. All othertrademarks herein are marks of the Lonza Group or its
affiliates.
For Research Use Only.
Manufactured for Lonza Rockland, Inc.
 2011 Lonza Rockland, Inc.
All rights reserved.

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