Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18691-1007-12
Rockland, ME 04841 USAReliant® Gel System
Precast agarose minigels that save you time.
Peel, Press and Pour, and you’re ready to load yoursamples.
1. Peel the paper backing from the adhesive strips on thebottom of the tray.
2. Peel off the lid. Leave the gel in the tray.
3. Place the tray directly onto the chamber platform. Alignthe wells so the DNA samples will run straight. Press thetray to stick to the chamber platform.
4. Pour electrophoresis buffer (TAE or TBE) into thechamber to a depth of 5mm over the flange of the tray.*
5. Load your samples (<15 µl volume).
6. Electrophorese the gels at no more than 10 V/cm(interelectrode distance) for 30 minutes; lower voltages forlonger times are acceptable.
7. For DNA fragments >5 kb, use 1 to 5 V/cm (interelectrodedistance) and increase the run time.
8. Remove the gel from the tray to photograph/document
and/or stain/destain (for gels without ethidium bromide).

Introduction®

The Reliant Gel System consists of precast agarose minigels
for rapid and reproducible resolution of DNA sizes from 8 bp to>10 kb in length. Reliant® Gels are available in a variety ofwell formats and concentrations with and without ethidium®
bromide. Reliant Gels fit most mini and medium
electrophoresis chambers. Optimal results are achieved whenused with the Reusable UV Transparent Tray.
•••

Reliable – Made with high quality molecular biology grade®
®
SeaKem or NuSieve Type Agaroses.
Easy to use – Remove the lid, peel away the tape, apply
tray to the electrophoresis chamber, flood with buffer, andload samples.
Fast – Results can be seen in 30 minutes or less.

Specifications

Tray dimension:
Gel dimension:
Gel thickness:
Number of wells:
Well volume:
Agarose:Gel performance:

6.8 x 10.2 cm
6.0 x 9.5 cm
5.5 mm8, 12, 18, 20 and 24<15 µlNo detectable DNase or RNase
Sharp DNA bands* For best band resolution, add 0.5 µg/ml ethidium bromide tothe running buffer.
Caution: Ethidium bromide is a mutagen. Use gloves, lab
coat and eye protection when handling solutions or gels
containing the dye. Avoid skin and eye exposure to UVlight. Refer to Material Safety Data Sheet for complete
safety and handling information.

Storage Conditions
18ºC-24ºC for 6-12 months depending upon agarose
concentration.

Dye Mobility
Agarose Type &Concentration

Electrophoresis Chambers
Reliant Gels in the portrait format (8,12, and 24 well) will fit
most minichambers, and all medium/large chambers.
For information on chambers for the landscape format, pleaserefer to the Lonza Catalog, or contact Lonza Technical Serviceat 800-521-0390 or visit our web site www.Lonza.com.

BromophenolBlueXylene
Cyanol760 bp250 bp40 bp

6,100 bp
1,500 bp
350 bp500 bp100 bp20 bp

4,000 bp1,000 bp
250 bp

1X TAE Buffer
1% SeaKem® Gold Agarose
2% SeaKem® Gold Agarose
4% NuSieve® 3:1 Plus
AgaroseIt is sometimes difficult to see all the wells through theback of the tray, don’t be alarmed. Some wells may haveair pockets and will be more visible while other wells maynot.

1X TBE Buffer
1% SeaKem® Gold Agarose
2% SeaKem® Gold Agarose
4% NuSieve® 3:1 Plus
AgaroseTechnical TipReliant® Gels are designed to be electrophoresed(10 V/cm for 30 minutes) in the packaging tray. By modifying
the tray as described in steps 1-4 and decreasing the amountof buffer overlay you can achieve similar resolution in less than
30 minutes!

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Instructions for Tray Modification
1.

2.
3.
4.
5.

Remove the gel from the tray.
If gel contains ethidium bromide or gels are for RNA
wear gloves.
Using sharp, heavy scissors, make one vertical cut in eachcorner of the tray.
Fold down the ends and cut across.
The modified tray has no lip and an edge <3 mm, enoughto hold a gel in place during electrophoresis.
Pour the buffer into the chamber to a depth of ~3 mm over
the top of the gel.

Instructions are for the 8-, 12-, or 24-well format.
For 18- and 20-well formats, make the cuts on the long sides of
the tray. The modified tray edge should be parallel with theelectrodes when placed in the chamber.
Lonza offers UV Transparent Trays that do not require theabove modifications, please see ordering information on
the back page, or contact Technical Service at(800) 521-0390 or biotechserv@Lonza.com for more
information.

Gel Running Protocol using modified Tray
1. Peel the paper backing from the adhesive strips positionedon the back of the midified tray.

2. Press the modified tray directly onto the chamber platform.
3. Each time you run a Reliant® Gel, remove the gel from its
tray, and place it in the modified tray holder.

4. Use a minimal amount of buffer over the top of the gel~3 mm.

5. Load your samples. Electrophorese at 10 V/cm,(interelectrode distance).

6. To avoid loss of small fragments, stop the electrophoresis
before the dye front reaches the edge of the gel.

7. Tray holder is good for several runs. Replace the tray
holder when adhesive backing starts to loosen from thechamber’s platform.

Related Products

AccuGENE® TAE, TBE, and MOPS Buffers
RNA Markers
DNA Markers
DNA Ladders
Latitude® Gels
Latitude™ Midigel Chamber®
FlashGel System

For more information contact Technical Service at(800) 521-0390 or visit our website atwww.Lonza.com
For Laboratory Use.

SeaKem is a trademark of FMC Corporation. All othertrademarks herein are marks of the Lonza Group or its
affiliates.
© 2007 Lonza BioScience Rockland, Inc.
All rights reserved.

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