DNA and RNA

Purification protocols for samples collected in
OMNIgene™•SALIVA DNA and RNA (OMR-610)
OMNIgene™•SALIVA DNA and RNA (OMR-610) purification protocol forhuman and bacterial nucleic acids using RNeasy® PowerMicrobiome® Kit
This laboratory protocol is used for the preparation of a saliva sample collected and stabilized in
OMNIgene™•SALIVA DNA and RNA (OMR-610) for subsequent extraction of human and bacterial
nucleic acids (DNA and RNA) and viral DNA using the RNeasy® PowerMicrobiome® Kit.1

Equipment and reagents••RNeasy PowerMicrobiome Kit (QIAGEN®, Cat No./ID: 26000-50)
Equipment and reagents listed as referenced in the RNeasy PowerMicrobiome Kit Handbook†– For a detailed extraction protocol, refer to the handbook included in the extraction kit.

Procedure
Sample preparation steps
1. Shake OMR-610 samples vigorously for 10 seconds once received in the lab.
2. Incubate the entire sample in the original vial at 50°C for 1 hour in a water bath or for 2 hours in anair incubator.
Note: Incubation may be performed any time between sample receipt and extraction.
This step does not need to be repeated for extraction of subsequent aliquots.
3. Add 100 µL phenol-chloroform-isoamyl alcohol (pH 6.5-8.0) to the PowerBead Tube.
4. Transfer 250 µL of sample into the Glass Bead Tube from the RNeasy PowerMicrobiome RNA Isolation Kit.
5. Continue with the RNeasy PowerMicrobiome RNA Isolation Kit instructions, starting at step 2.
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PD-PR-01363 Issue 1/2023-02© 2023 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved.

DNA and RNA

OMNIgene•SALIVA DNA and RNA (OMR-610) purification protocol for viral
RNA using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit
This laboratory protocol is used for the preparation of a saliva sample collected and stabilized inOMNIgene•SALIVA DNA and RNA (OMR-610) for subsequent extraction of viral RNA using the MagMAX™
Viral/Pathogen Nucleic Acid Isolation Kit.

Equipment and reagents••

MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Applied Biosystems™, Cat. No./ID: A42352)
Equipment and reagents listed as referenced in the MagMAX Viral/Pathogen Nucleic Acid Isolation
Kit User Guide– For a detailed extraction protocol, refer to the user guide included in the extraction kit.

ProcedureSample preparation steps
1. Shake OMR-610 samples vigorously for 10 seconds once received in the lab.
2. Incubate the entire sample in the original vial at 50°C for 1 hour in a water bath or for 2 hours in an
air incubator.
Note: Incubation may be performed any time between sample receipt and extraction.
This step does not need to be repeated for extraction of subsequent aliquots.
3. Continue with MagMAX Viral/Pathogen Nucleic Acid Isolation Kit User Guide, starting at step 1.

Technical support is available Monday to Friday (9:00 a.m.-5:00 p.m. ET):•••

Toll-free (North America): 1.866.813.6354, option 6
All other countries: 613.723.5757, option 6
Email: support@dnagenotek.com

1Formerly sold by MO BIO as PowerMicrobiome RNA Isolation Kit (26000-50)†RNeasy® PowerMicrobiome® Kit Handbook, HB-2276-003, January 2020. (www.qiagen.com)
Some DNA Genotek products may not be available in all geographic regions.
OMNIgene•SALIVA DNA and RNA (OMR-610) is for Research Use Only, not for use in diagnostic procedures.
OMNIgene and DNA Genotek are trademarks of DNA Genotek, Inc. All other brands and names contained herein are the property of their respective owners.
All DNA Genotek protocols, white papers and application notes are available in the support section of our website at www.dnagenotek.com.

Not available for clinical diagnostic use in the United States.
PD-PR-01363 Issue 1/2023-02© 2023 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved.