7th Asia-Pacific Conference on Plasma Physics, 12-17 Nov, 2023 at Port Messe Nagoya

Generation and Characterization of Cold Atmospheric Pressure Plasma Jetand Its Applications

H. B. Baniya1⁎, R. P. Guragain2, S. Dhungana3 and D. P. Subedi2
Department of Physics, Amrit Campus, Tribhuvan University, Kathmandu, Nepal.
2
Department of Physics, School of Science, Kathmandu University, Nepal.
3
Central Department of Physics, Tribhuvan University, Kirtipur, Kathmandu, Nepal
1⁎Corresponding Author: hombaniya@gmail.com/hom.baniya@trc.tu.edu.np

Cold atmospheric pressure plasma jet (CAPPJ)
has many applications in biomedicine and polymer
surface modification to enhance adhesion properties.
Using argon as a carrier gas, the gas temperature,remains at room temperature even after hours ofoperations. The plasma jet can be touched by hands anddirected manually by a user to bring in contact with
heat-sensitive objects and materials including skinwithout causing any heating or painful sensation. CAPPJ
has been generated by a high voltage power supply (0-20kV) at an operating frequency of 25 kHz.
Thedischarge has been characterized by electrical and opticalmethods. In order to characterize the plasma jet, its
electron temperature and electron density have been
determined by optical emissions spectroscopy. This paper
reported the antimicrobial and anticancer property ofcold atmospheric pressure plasma.
The result indicates that CAPPJ is capable ofkilling the microbial population in its exposed area.
Plasma jet was used to treat selected strains ofpathogenic bacteria for varying voltage (4-6 kV and 25kHz) and varying time duration.
Cold plasma used to treat normal and cancer celllines (at working voltage 13 kV and line frequency 50Hz) to show strong anticancer property of plasma. The
reactive species present in the cold plasma jet:high-energy electrons, ionized atoms and molecules, andUV photons are the key factors that cause an effective
reduction in the number of microorganisms and killed
cancer cells as well with compared to control cells. The
cold plasma treatment selectively killed cancer cells
without affecting normal cells in vitro. It has been
observed that the percentage viability of the cell lines
varies with the plasma treatment time along the best fitted
curve of a power function.
The curve is steeper for the cancer cells than for thenormal cells after plasma treatment. The faster decaying
curve signifies the selective killing of the cancer cells
compared to the normal cells within the exposure time.
This study indicates that the reactive oxygen species in theCAPP activate the apoptosis pathway in the cancer cells

2.

Baniya HB, Shrestha R, Guragain RP, Kshetri MB,
Pandey BP, Subedi DP. Generation andcharacterization of an atmospheric-pressure plasmajet (APPJ) and its application in the surface
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F, Brinckmann S, Steinborn E, Havenith M,Benedikt J, Bandow JE. Photons and particles
emitted from cold atmospheric-pressure plasma
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5. Baniya HB, Guragain RP, Baniya B, Subedi DP.
Cold atmospheric pressure plasma jet for theimprovement of wettability of polypropylene. Int JPolym Sci. 2020; 1-9.
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Figure xx

Key words:
Cold atmospheric pressure plasma jet, Antibacterial
property, Anticancer property of plasma, Efficacy,
Reactive oxygen and nitrogen (RON) species
References
1. Hong YC, Uhm HS. Microplasma jet atatmospheric pressure. Appl Phys Lett. 2006;
89(22):221504.

Experimental Setup