Presentation Guidance

SPDP WS 2023

General Presentation Guidance

Call for Papers• Oral Presentation
Research for / with nonhuman primates• CPC (Clinico Pathology Conference)
Discussion for spontaneous cases in nonhuman primates• Poster Presentation
Research for / with nonhuman primates

Abstract Submission• By

Fri, May 31, 2024

• To

SPDP O ce; spdp.itagaki@gmail.com

Abstract Format•

Essential: Title，Name of presenter and co-worker(s), A liation(s) of presenter and coworker(s), E-mail address of presenter, Description•

Languages:Japanese or English•

See the example abstract for details.
- Put “◦” on the name of presenter.
- Descriptions shall be concisely arranged with labels such as “Introduction”, “Materialsand Methods”, “Results” and “Discussions”.
- Document format such as fonts, font size, line height etc. shall be arranged by aneditor for the proceeding.
- Speci c characters may be replaced without changing of the original meanings.

• Upper limit:

Up to 2 pages in A4 size.

• Acceptable le format:
PDF, Plain text, Rich text, MS-Word, Apple Pages, ODF document

Presentation
Notice me the site of your presentation; ON SITE or ON LINE, also what device will you use;Your own device or the Venue prepared if you were on site.

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Presentation Guidance

Poster Presentation
1. Face to Face participant
Prepare a printed poster (P-poster) for the venue hall and its digital le for online
participants.
2. Online participant
Prepare a E-poster.
Presentation period•E-posterFri, July 5 to Sun, 7, 2024•

P-posterOn the day of Workshop.

Discussion / Q&A•

E-poster

On a mailing list for the participants.
Discussion can be continued directly with the presenter andthe questioner after the online presentation period.

•

P-poster

Anytime on the venue.

Preparation
1. P-poster• SizeA0, vertical position• Poster No.

A distributed poster No. shall be shown on the head of the poster title.

• Submission

Bring your own P-poster to the venue.

2. E-poster• File format

PDF• File size
Page format

Less than 30MB
A size, vertical position (W:H = 1 : √2)• Poster No.

A distributed poster No. shall be shown on the head of the poster title.

• Submission
To:

spdp.itagaki@gmail.com

Via:

Large les transfer service or File sharing on your cloudMore information / Contact
Iori ITAGAKIThe Society of Primate Diseases and Pathology.
spdp.itagaki@gmail.comfifi2

3D-Quanti cation of Abdominal Fat Using MRI in Cynomolgus Monkeys○ Iori ITAGAKI1, Hiromi OGAWA1, Hayato NARITA1, Hiroaki SHIBATA2
1 The Corporation for Production and Research of Laboratory Primates, 2 Tsukuba Primate

Research Center, National Institute of Biomedical Innovation
E-mail address: itagaki@primate.or.jp
Introduction: This study is to establish an analytical method for quanti cation ofabdominal fat tissue based upon actual three-dimensional (3D) con guration of
intraabodominal fat (IaF) and subcutaneous fat (ScF) in cynomolgus monkeys using magnetic
resonance imaging (MRI) technique. Excess calories and hypokinetic lifestyle commonly
result in obesity. It is also usual with this species of monkey, especially in husbandry. Obesemacaques are unique model of human obesity and occasionally die a sudden without priorindication of illness or following brief weight loss (Fatal fasting syndrome).
Materials and methods:
Ten female cynomolgus monkeys have been kept individually,
10 to 25 years old in age and 3.10 to 6.80 kg in body weight, were anesthetized by iso urane
inhalation with monitoring of vital signs. Then abdominal portion of these animals werescanned by Allegra 3.0T with syngo MR A30 for operation software (SIEMENCE, Germany)to get three types of spin-echo T1-weighed images (T1WI) in the following conditions;
T1WI-1:
T1WI-2:
T1WI-3:

Slice thickness 2.0mm, Repetition time 500ms, Echo time 15ms, in sagittal
sectionSlice thickness 2.0mm, Gap between slices 2.0mm, Repetition time
1,640ms, Echo time 9.5ms, without Water suppression, in coronal section
Slice thickness 2.0mm, Gap between slices 2.0mm, Repetition time
1,640ms, Echo time 9.5ms, with Water suppression, in coronal section

Tissues of IaF and ScF were quanti ed with ImageJ 64 (National Institute of Health, USA) on
sequencial 30 of T1WI-3 which were identi ed as upper than lumbosacral junction in referredwith T1WI-1. T1WI-2 were referred for anatomic understanding. The MR images wereconverted to 16-bit grayscale stacks in 768 x 768 pixel resolution (768 pixels = 210.00 mm),then following two types of image in 8-bit grayscale were abstracted using Binary command;(A)(B)

T1-high area of both IaF and ScF
An area formed by manually traced line roughly placed between IaF and ScF

IaF and ScF areas (mm^2) were measured on the images which were obtained by thefollowing image calculations;

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Image IaF = Binary image (A) - Binary image (B)Image ScF = Binary image (A) - Image IaF

Then the volumes (mm^3) were calculated by the following formulae;
Volume IaF (mm^3)
Volume ScF (mm^3)= 4 mm x ∑1-30 Area of IaF (mm^2)= 4 mm x ∑1-30 Area of ScF (mm^2)

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Results:
Using above mentioned technique, high-contrasted MR images of abdominal
fat tissues in cynomolgus monkeys were successfully obtained. They were easy to process andsuitable for 3D image analysis with an open-source image analyzing software. The total
abdominal fat volumes analyzed by this method, which is based upon actual anatomic
distribution of fat tissues, well correlated with the body weight (BW) values and body massindexes (BMIs) expediently computed from BW and crown-rump (C-R) length, and wereconsidered as accurate re ection of actual state of body fat accumulation of each monkey.
Discussions: This newly developed method can take place the current major way of body fat
quanti cation using Dual Energy Absorptiometry (DEXA) which is based upon twodimensional imaging in primate models for human obesity.