Life Science Education

Solutions for Teaching PCR

Visit bio-rad.com/explorer for more information.

TEACH PCR — THE CORE TECHNIQUE
OF MODERN MOLECULAR BIOLOGYPolymerase chain reaction (PCR) is a fundamental technique used to create copies
of specific bits of DNA. Teaching your students how to perform PCR equips themwith skills crucial to fields ranging from genetic engineering, diagnostics, andpersonalized medicine to evolutionary and conservation biology. By partnering withBio-Rad, you can ensure your students learn these skills using the same equipment
and reagents used in research labs around the world.

Bio-Rad Can Help
An industry leader in the development of cutting-edge products for biologicalresearch and diagnostics, Bio-Rad expresses its commitment to science education
through its Explorer program, which offers Bio-Rad products at a discount for
use in classroom laboratories. This ensures students learn modern biotechnology
techniques like PCR using the same equipment they might someday use in researchand diagnostics labs. The Explorer program also partners with teachers and industry
experts to develop educational kits and training resources to help you achieve yourteaching goals.

Educational Kits for PCR
Teach PCR with these hands-on lab activities that engage students with real-world contexts. Students amplify and analyze DNA fromforensic (simulated), human, agricultural, and environmental samples to explore the utility of PCR in crime scene investigation, medicine,food analysis, and ecology, among other applications.

Crime Scene Investigator PCR Basics Kit
Students use premade DNA samples tosimulate DNA profiling using short tandem
repeat (STR) analysis, as commonly used inforensic laboratories.

GMO Investigator Kit
Students extract DNA from food samples andthen apply PCR and DNA electrophoresis totest for the presence of two GMO-associated
DNA sequences.

PV92 PCR Informatics Kit
Students extract DNA from their cheekcells or hair follicles and then use PCR andelectrophoresis to fingerprint their own DNA.
Explore the phenomenon of Alu repeats andgive students a personal connection to theHardy-Weinberg equilibrium.

DNA Barcoding Kits
Students incorporate DNA extraction, PCR,DNA sequencing, and bioinformatics as they
genetically identify species of fish, mammals,
insects, or fungi using a DNA barcode.
Investigate local biodiversity or check to
see whether food fraud is occurring.

2 | BIO-RAD LABORATORIES, INC.

Research-Quality Equipment for PCR
T100 Thermal Cycler
A PCR workhorse in academic and industrial research labs, theuser-friendly T100 Thermal Cycler offers the reliability, durability,and industry-leading performance that you expect from Bio-Rad.
Capable of running up to 96 samples all at once, the T100 ThermalCycler is easy to program and accommodates standard PCR
tubes, tube strips, and plates.

PTC Tempo Thermal Cycler
For educators looking for more advanced features andflexibility, the PTC Tempo Thermal Cycler is available in various
configurations to meet different throughput needs. It offers LED
run status lights, a programmable thermal gradient, and advanced
connectivity options with WiFi, network drive access, and BR.io
cloud platform accessibility.

FeatureT100 Thermal Cycler
Robust and economical for routine PCR

Format(s)96-well

User interface
Performance

5.7" graphical touch screen■ Accuracy: ±0.5°C■ Uniformity: ±0.5°C well-to-well within 30 sec■ Max ramp rate: 4°C/sec■ Temperature range: 4–100°C
26 x 47 x 23 cm (10 x 18 x 9")—<500 protocols; unlimited with a USB drive

Size (W x D x H)
Connectivity
Memory capacity

PTC Tempo Thermal Cycler
For demanding applications and multiple users, offers
premium performance on a modern platform■ 96-well■ 48/48-well■ 384-well■ Deepwell8" graphical touch screen■ Accuracy: ±0.2°C■ Uniformity: ±0.4°C well-to-well within 10 sec■ Max ramp rate: up to 5°C/sec■ Temperature range: 4–100°C
28 x 50 x 26 cm (11 x 20 x 10")
WiFi, BR.io, USB A 2.0, Ethernet, barcode scanner<100,000 protocols; unlimited with a USB drive

Cloning and Sequencing Explorer Series

Teach a complete gene cloning workflow with this 6- to 8-week series of laboratory
activities that encompass nucleic acid extraction, PCR, cloning into a plasmid, DNA
sequencing, and informatics.

Options for Teaching PCR Applications without a Thermal Cycler
Virus Detection and Transmission Kit
Use preamplified DNA samples (no thermal cycler needed) and gel electrophoresis todeduce the mode of transmission and chain of infection of a virus throughout a restaurant.
Science of Opioid Dependence Kit
Have your students explore the environmental and genetic links to opioid dependence
as they design a human genetic research study using preamplified and predigested
DNA samples.

BIO-RAD LABORATORIES, INC. | 3

Have Questions? Need a Quote?
Visit explorer.bio-rad.com to learn more about products and resources for educators.
Contact us at info.bio-rad.com/ExplorerContactUs with any other questions or to request a quote.
Ordering Information (Educator Discount)
The ordering information listed here is for purchases for educational use only.
Visit bio-rad.com/PCR for information on ordering for research use.
Catalog #Description

Thermal Cyclers
1861096EDU
T100 Thermal Cycler, includes 96-well thermal cycler, power cord,
tube support ring12015382EDU PTC Tempo 96 Thermal Cycler, network-connected thermal
cycler for PCR, includes PTC Tempo 96-well instrument, cables
12015392EDU

12015394EDU
12015309EDU
12018650EDUPTC Tempo Deepwell Thermal Cycler, network-connected
thermal cycler for PCR, includes PTC Tempo Deepwell instrument,
cablesPTC Tempo 384 Thermal Cycler, network-connected thermalcycler for PCR, includes PTC Tempo 384-well instrument, cables
PTC Tempo 48/48 Thermal Cycler, network-connected thermal
cycler for PCR, includes PTC Tempo 48/48-well instrument, cables
PTC Tempo Thermal Cycler WiFi Adapter, U.S. only, WiFi
antenna enables WiFi connection for PTC Tempo Thermal Cycler inthe United States

12018509EDU

PTC Tempo Thermal Cycler WiFi Adapter, global, WiFi antenna
enables WiFi connection for PTC Tempo Thermal Cycler
Educational Kits for PCR
1662600EDU
Crime Scene Investigator PCR Basics Kit, contains sufficientmaterials for 8 student workstations (2–4 students per workstation)
1662100EDU
PV92 PCR Informatics Kit, contains sufficient materials for 8student workstations (2–4 students per workstation)
1662500EDU
GMO Investigator Kit, contains sufficient materials for 8 student
workstations (2–4 students per workstation)
17007432EDU Fish DNA Barcoding Kit, contains sufficient materials for 8 student
workstations (2 samples and 2–4 students per workstation)
17007366EDU

17007154EDU

17008261EDU

Catalog #Description

Reagents and Consumables for PCR
1665009EDU

Master Mix for PCR, 1.2 ml

1708874EDU

dNTP Mix, 0.2 ml

1708872EDU

MgCl2 Solution, 1.25 ml

1708870EDU

iTaq DNA Polymerase, 250 U

TBS0201EDU

0.2 ml 8-Tube Strips without Caps

TLS0801EDU

Low-Profile 0.2 ml 8-Tube Strips without Caps, natural

TLS0851EDU

Low-Profile 0.2 ml 8-Tube Strips without Caps, white

TCS0803EDU

Optical Flat 8-Cap Strips

TFI0201EDU

0.2 ml PCR Tubes with Flat Caps

TWI0201EDU

0.2 ml PCR Tubes with Domed Caps

BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. All trademarks used herein are theproperty of their respective owner. © 2023 Bio-Rad Laboratories, Inc.

Mammals, Insects, and Fungi DNA Barcoding Kit, contains
sufficient materials for 8 student workstations (2 samples and2–4 students per workstation)
Fish, Mammals, Insects, and Fungi DNA Barcoding Kit,
contains sufficient materials for 8 student workstations(2 samples and 2–4 students per workstation)
Virus Detection and Transmission Kit, contains sufficientmaterials for 8 student workstations (2–4 students per workstation)

17005316EDU

Science of Opioid Dependence Kit, contains sufficient materials
for 8 student workstations (2–4 students per workstation)

1665000EDU

Cloning and Sequencing Explorer Series, includes 8 modules
with lab activities from DNA extraction and cloning to computerbased sequence analysis, curriculum

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