Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18106-0807-7
Rockland, ME 04841 USA

SeaKem® Gold Agarose
The fastest agarose available for separation of megabase DNA.
Precautions
Always wear eye protection when dissolving agaroseand guard yourself and others against scalding
solutions. Refer to Material Safety Data Sheet foradditonal safety and handling precautions.

Introduction
SeaKem® Gold Agarose is a unique, patented, very highgel strength, low EEO (<0.05) standard gellingtemperature agarose. This Genetic Technology Grade™
Agarose product was developed for rapid resolution ofDNA and PCR† products between 1 kb and 50 kb by
conventional electrophoresis.

Microwave lnstructions for Agarose Preparation

Analytical Specifications
Gelling temperature (1.5%)
Melting temperature (1.5%)
Gel strength (1%)
Gel strength (1.5%)

36°C ±1.5°C>90°C>1,800 g/cm²>3,500 g/cm²

1. Choose a beaker that is 2-4 times the volume of thesolution.
2. Add room temperature 1X or 0.5X electrophoresis
buffer and a stir bar to the beaker.
3. Slowly sprinkle in the agarose powder while thesolution is rapidly stirred.
4. Remove the stir bar if not Teflon® coated.
5. Weigh the beaker and solution before heating.
6. Cover the beaker with plastic wrap.
7. Pierce a small hole in the plastic wrap for ventilation.
8. Heat the beaker in the microwave oven on Highpower until bubbles appear.
9. Remove the beaker from the microwave oven.
Caution: Any microwaved solution may becomesuperheated and foam over when agitated.
10. GENTLY swirl the beaker to resuspend any settled
powder and gel pieces.
11. Reheat the beaker on HIGH power until the solution
comes to a boil.
12. Hold at boiling point for 1 minute or until all of theparticles are dissolved.
13. Remove the beaker from the microwave oven.
14. GENTLY swirl the beaker to thoroughly mix theagarose solution.
15. After dissolution, add sufficient hot distilled water toobtain the initial weight.
16. Mix thoroughly.
17. Cool the solution to 50°C-50°C prior to casting.

Applications• Preparative DNA and RNA electrophoresis• Analysis of Long PCR† reactions• Separation and further manipulation of DNA>1,000 bp• Analytical electrophoresis of DNA and RNA• Blotting of DNA and RNA
Suggested Agarose Concentrations
Size Range

Final Agarose Concentration (%)(Base Pairs)

1X TAE Buffer

5,000-50,000
1,000-20,000
800-10,000
400-8,000

0.3
0.5
0.8
1.0

E TBE Buffer is not recommended for separation of DNA >12,000 bp.

Dye Mobility Table
Migration of double-stranded DNA in relation toBromophenol Blue (BPB) and Xylene Cyanol (XC) in
SeaKem® Gold Agarose Gels.
1X TAE Buffer%

1X TBE Buffer

XC

BPBAgarose

XCBPB

24,80012,200
9,200
6,100
4,100
2,600
2,000
1,500

3,550
2,050
1,050
760
600
400
330
250

0.30
0.50
0.75
1.00
1.25
1.50
1.75
2.00

19,000
9,200
7,100
4,000
2,550
1,900
1,400
1,000

2,550
1,500
800
500
350
250
180100Hot Plate Instructions for Agarose Preparation
1. Choose a beaker that is 2-4 times the volume of thesolution.
2. Add room temperature electrophoresis buffer and astir bar to the beaker.

13. Slowly sprinkle the agarose powder while thesolution is rapidly stirred.
4. Weigh the beaker and solution before heating.
5. Cover the beaker with plastic wrap.
6. Pierce a small hole in the plastic wrap for ventilation.
7. Bring the solution to a boil while stirring.
8. Maintain gentle boiling until all the agarose isdissolved (approximately 10 minutes).
9. Add sufficient hot distilled water to obtain the initial
weight.
10. Mix thoroughly.
11. Cool the solution to 50°C-60°C prior to casting.

Ordering Information:
Catalog No.

Size

50150
50152

125 g
25 g

Related Products:

AccuGENE® 10X TAE Buffer
GelStar® Nucleic Acid Gel Stain
Megabase DNA Standards
InCert® Agarose†

The PCR process may be covered by one or more third-party
patents.

For Laboratory Use.
For more information contact Technical Service at(800) 521-0390 or visit our website atwww.Lonza.com

SeaKem and GelStar are trademarks of FMC Corporation. Teflon is atrademark of E.I. duPont de Nemours and Co. All other trademarks
herein are marks of the Lonza Group or its affilites.
© 2007 Lonza Rockland, Inc.
All rights reserved.

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