Product Catalog

Life Science Research

2023

Visit bio-rad.com for more information.

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Overview
Life Science Research 2023
Workflows for Translational Researchii

COVID-19 Solutions

xvi

New Products

xviii

Sample Preparation

1

Next-Generation Sequencingi

19

Sample Quantitation

27

Cell Counting, Enumeration, and Enrichment

33

Flow Cytometry

39

Cell Imaging

79

Chromatography: Laboratory and Process Separationsi83

Electrophoresis and Blotting

179

Antibodies

271

Imaging Instruments, Analysis, and Software

283

Bio-Plex Multiplex System

291

Microplate Systems

323

Transfection

327

DNA Amplification/PCR

341

Plastic Consumables and General Laboratory Equipment

417

Bio-Rad Explorer Educational Products

433

Appendices445

Note: Due to varying product timelines, some new items in this catalog may not be available for immediate purchase. Some older products may have been discontinued since thiscatalog’s creation. For more information, contact your local Bio-Rad sales representative.

Workflows for Translational Research
The journey of every therapeutic is unique. Bio-Rad offers a suite of customizable products
and services that ensure efficiency, reproducibility, and reliability at each step of discovery,
development, and manufacturing. With more than 70 years of experience as a global
leader in life science research and clinical diagnostics, Bio-Rad provides the highest-quality
integrated platforms and solutions so that you can focus on your science. Products are
manufactured in ISO 13485–certified production facilities and an experienced global support
team is available to partner with you to provide technical and regulatory audit support.

More than products: A trusted global partner
Bio-Rad’s commitment to your success does not end with your purchase. Our global
support and service team is dedicated to limiting downtime and maximizing your productivity.

Bio-Rad headquarters

Therapeutic development workflows — Bio-Rad understands the power of an optimized
workflow: systems, reagents, and software that work better together to enhance productivity
and promote confidence in your science. Over the next few pages, explore examples of thevarious solutions Bio-Rad has to offer.

ii | Workflows for Translational ResearchCustomizable products and services fromdiscovery to approval and releaseTargetDiscovery

Lead
Discovery

PreclinicalResearch

ClinicalResearch

Process
Development

Manufacturing &Quality Control

VaccineDevelopment

Protein-Based
Immunotherapy
Development

Biosimilar
Development

Cell and
Gene Therapy
Discovery

Workflows for Translational Research | iii

Gene Editing1

2

TRANSFECTION

ENRICHMENT AND
SINGLE-CELL ISOLATION

Successful gene editing starts with delivering theclustered regularly interspaced short palindromic
repeats with associated protein 9 (CRISPR-Cas9)
system to your cells of interest, whether animalor human, tumor-derived or wild-type. From
the Gene Pulser Xcell electroporation system toTransFectin lipid reagent, Bio-Rad offers industryleading solutions for transfection of even themost sensitive cell lines. Automate cell counting
with the TC20 automated cell counter and use
the ZOE fluorescent cell imager to monitor cell
health right on your bench.

Speed up your workflow, reduce passaging,and minimize unnecessary spending on
reagents and consumables by enriching yourcells of interest. With the ZOE fluorescent cell
imager, visualization is easily performed at thebench. The Genesis cell isolation system andCelselect Slide technology enable the enrichment
and retrieval for downstream applications orenrichment and staining for enumeration.

Gene Pulser Xcell
electroporation system,p. 332

TC20 automated cell counter,p. 34

Related Products

Genesis system,
p. 36

ZOE fluorescent cell imager,
p. 80

Related Products

Helios gene gun system,p. 336

Gene Pulser electroporationbuffer, p. 331

TC20 automated cell counter,p. 34

Celselect Slide technology
p. 37

Gene Pulser/MicroPulser
electroporation cuvettes,
p. 334

ZOE fluorescent cell imager,
p. 80

Cell counting slides for TC20
cell counters, p. 35

Flow cytometry antibodies,pp. 272, 281

TransFectin lipid reagent,p. 330Genesis system, p. 36

ReadiLink antibody labelingkits, p. 52

iv | Workflows for Translational Researchbio-rad.com

Gene Editing

3

4

CONFIRMATION OF EDITSDOWNSTREAM ANALYSIS

Confirmation of successful gene editing isa key step in any CRISPR-Cas9 workflow.
Bio-Rad offers a spectrum of solutions for direct
detection of edits by high-fidelity PCR, real-time
PCR, and high resolution melt analysis. For
unmatched precision and sensitivity, leverage
Droplet Digital PCR (ddPCR) to identify mutations
present even at frequencies ≤0.5%. For
confirmation at the protein level, the Stain-Free
western blotting workflow offers fast andaccurate quantitation.

Bio-Rad provides tools to study your newly
generated cell lines at the cellular, genomic, andproteomic levels. The flexible ZE5 cell analyzer
can be run by novices and experts alike to study
the effects of your edits at the cellular level.
Save time by analyzing up to 100 biomolecules
in a single sample with the Bio-Plex 200 system.

Stain-Free western blotting
workflow,
p. 232

QX600 Droplet Digital
PCR system,
p. 342

Related Products

Bio-Plex assays,p. 299ZE5 cell analyzer,
p. 45

Related Products

ddPCR genome edit
detection assays, p. 356

Precision melt supermix,
p. 390

CFX Opus real-time PCRsystem, p. 370

ReadiLink antibody labelingkits, p. 52

ddPCR enhancer reagent A,
p. 363

PrecisionAb validated
western blotting antibodies,p. 273

QX ONE Droplet Digital PCR
system, p. 344

PrecisionAb validated
western blotting antibodies,p. 273

CFX Opus real-time PCRsystem, p. 370

Antibodies, p. 272

PX1 PCR plate sealer,p. 400StarBright Dye labeled
antibodies, p. 276

CFX Maestro software,
p. 373

C1000 Touch thermal cycler,p. 365

PrimePCR assays, p. 376

PX1 PCR plate sealer,p. 400

iTaq universal supermixes,
p. 383

Flow cytometry antibodies,pp. 272, 281

PrimePCR assays, p. 376

iProof HF master mix, p. 398

PureZOL RNA isolation
reagent, p. 11

SsoAdvanced universal
probes supermix, p. 381

GelDoc systems, p. 287

Reliance one-step multiplex
RT-qPCR supermix, p. 385

Bio-Plex 200 system, p. 293

QX600 Droplet Digital PCR
system, p. 342

Stain-Free western blotting
workflow, p. 232

EveryBlot blocking buffer,
p. 245

bio-rad.com

Aurum total RNA kits, p. 10

Antibodies, p. 272

HuCAL® antibodies, p. 272

Workflows for Translational Research | v

Vaccine Development

1

2

DISCOVERYPROCESS DEVELOPMENT

Evaluating the efficacy of a candidate vaccine
requires analysis of diverse proteins and cell
types over time. With Bio-Plex assays, you
can easily monitor multiple proteins at once
to determine whether the immune system
is responding appropriately. For cell-based
analysis, Bio-Rad offers the automation-friendly
ZE5 cell analyzer, which can measure up to 30
parameters at once and detect rare events andcell types.

Method development is required to create
and optimize a purification process that
produces a vaccine candidate with sufficient
purity and yield. Bio-Rad offers a varietyof chromatography resins scalable fromdiscovery to production. These resins provide
purification benefits spanning a range of
biomolecules used in vaccine development,
including new selectivity and high capacity to
improve recovery and purity. Additionally, theNGC chromatography platform can be used
to automate purifications from discovery toprocess development.

Bio-Plex assays,p. 299ZE5 cell analyzer,
p. 45

Related Products
Antibodies, p. 271
Bio-Plex cytokine assays,p. 300Resins,pp. 85, 172

NGC chromatographysystems, p. 150

Related Products
Flow cytometry antibodies,pp. 272, 281

Stain-Free western blotting
workflow, p. 232

Foresight Pro columns,
pp. 132, 175

ReadiLink antibody labelingkits, p. 52

ChemiDoc imaging systems,
p. 285

Stain-Free gels,
pp. 196, 203

Foresight chromatography
resin screening tools,
p. 173

EconoFit columns, p. 127

Process chromatography
resins, pp. 172, 173

vi | Workflows for Translational Researchbio-rad.com

Vaccine Development

3

4

PURITY ANALYSISQUALITY CONTROLAs you develop a purification scheme, youwill need to assess the purity of the vaccine.
The GS-900 calibrated densitometer is designed
for use in regulated environments and has
IQ/OQ and U.S. FDA 21 CFR Part 11 compliant
software tools. To ensure that the correct proteinsare expressed at the desired levels, Bio-Rad
offers premade conventional and custom humanrecombinant antibodies for quantitative enzymelinked immunosorbent assays (ELISAs).

Host cell contamination is an area of primaryconcern in vaccine manufacturing. The QX200
and QX ONE Droplet Digital PCR systems
provide semi-automated and automatedmethods for easy quantification of host cellDNA. Unlike quantitative PCR (qPCR), ddPCR
does not require a standard curve and provides
an absolute readout, which is preferred for
reduction of interanalyst variability in quality
control (QC). The Stain-Free western blotting
workflow can be used to confirm vector identityand expression of surface proteins.

GS-900 calibrated
densitometer,p. 288

HuCAL custom antibodies,p. 272

Related Products
Stain-Free western blotting
workflow, p. 232
Stain-Free gels,pp.196, 203

QX ONE Droplet Digital
PCR system,
p. 344

Related Products
TMB peroxidase substratekits, p. 325

ddPCR supermix for residual
DNA quantification, p. 351

CFX Maestro software,
p. 373

Vericheck ddPCR HEK293
res DNA quant kit, p. 361

PrecisionAb validated
western blotting antibodies,p. 273

Vericheck ddPCR HEK293
res DNA size kit, p. 362

TidyBlot reagent, p. 280

Vericheck ddPCR
Mycoplasma detection kit,p. 363Clarity and Clarity Max ECL
western blotting substrates,
p. 248

Vericheck ddPCR residual
DNA quantification kits,
p. 361

QX200 Droplet Digital PCR
system, p. 345

CFX Opus real-time PCRsystem, p. 370

bio-rad.com

Stain-Free western blotting
workflow,
p. 232

EveryBlot blocking buffer,
p. 245

Workflows for Translational Research | vii

Cell and Gene Therapy Development

1

2

FOUNDATIONAL BIOLOGYPRECLINICAL/CLINICAL

Developing new cell or gene therapies starts
at the bench. The automation-friendly ZE5cell analyzer is capable of measuring up
to 30 parameters at once, enabling deep
characterization of cellular populations. Forprotein-based assays, Bio-Rad offers premade
antibodies and antibody sets. When off-theshelf antibodies don’t meet your needs, try
Bio-Rad’s custom, fully human monoclonal
antibodies. Custom antibodies from our phagedisplay libraries produce highly specific reagentsand can be generated in as little as eight weeks,saving valuable time.

Assessing the safety and efficacy of new
therapies requires analyses at the cellular, nucleic
acid, and protein levels. Leverage the powerof the QX ONE Droplet Digital PCR system
for absolute vector quantitation in prepared
therapies, treated cells, or treated patients. At
the cellular level, the ZE5 cell analyzer can beused to monitor changes in specific immune cell
populations over time. Detect adverse effects
with Bio-Plex assays, including predesigned
panels for simultaneous quantification ofcytokines and other immune-related proteins.

ZE5 cell analyzer,
p. 45

HuCAL antibodies,p. 272

Related Products

QX ONE Droplet Digital
PCR system,
p. 344

Bio-Plex 200 system,p. 293

Related Products

Flow cytometry antibodies,pp. 272, 281

CFX Opus real-time PCRsystem, p. 370

QX200 Droplet Digital PCR
system, p. 345

Reliance one-step multiplex
RT-qPCR supermix, p. 385

ReadiLink antibody labelingkits, p. 52

PrimePCR assays, p. 376

CFX Opus real-time PCRsystem, p. 370

Reliance Select cDNA
synthesis kit, p. xix, 394

ReadyPrep protein extraction
kit, p. 2

Aurum total RNA kits, p. 10
PureZOL RNA isolation
reagent, p. 11

ZE5 cell analyzer, p. 45

Bio-Plex assays, p. 299

PX1 plate sealer, p. 400

HuCAL antibodies, p. 272

Reliance Select cDNA
synthesis kit, p. xix, 394

PrimePCR assays, p. 376

Flow cytometry antibodies,pp. 272, 281

Stain-Free western blotting
workflow, p. 232
QX ONE Droplet Digital PCR
system, p. 344

viii | Workflows for Translational Research

Reliance one-step multiplex
RT-qPCR supermix, p. 385

SsoAdvanced universal
supermixes, pp. 380, 381

ddPCR cell and gene therapy
assays, p. 357

bio-rad.com

Cell and Gene Therapy Development

3A

3B

MANUFACTURING/QC —GENOMIC ASSAYS

MANUFACTURING/QC —ANTIBODY-MEDIATED ASSAYSAs a new therapy moves toward regulatory
approval, manufacturing processes must be
developed and validated for reproducibility
while maintaining product safety and efficacy.
Measuring viral titers for gene therapy or vector
copy number for gene-modified cell therapy withhigh accuracy and precision is a challenge withreal-time PCR. Use the QX ONE Droplet Digital
PCR system, which provides absolute
quantification, to deliver a reliable count ofvector genome copies. In addition, Bio-Rad
offers ddPCR residual DNA quantification kits
for checking purity with maximum sensitivity.

High-quality antibody reagents are vital for
determining the percentage of transfected
T cells prior to therapy administration and for
differentiating CAR T cells from other cells inpatient samples using flow cytometry. Bio-Rad'scustom antibody services use in vitro phagedisplay to generate highly specific antibodies
for these critical assays. These reagents canbe reproduced synthetically for batch-to-batch
uniformity and long-term security of supply.
Additionally, Bio-Rad's incorporation of SpyTagtechnology enables production of custom
antibodies in multiple formats for use in a variety
of applications.

QX ONE Droplet Digital
PCR system,
p. 344

Vericheck ddPCR
Mycoplasma detection kit,p. 363

Related Products

Custom Antibody Service,p. 272

HuCAL antibodies,p. 272

Related Products

QX200 Droplet Digital PCR
system, p. 345

Vericheck ddPCR HEK293
res DNA size kit, p. 362

Vericheck ddPCR residual
DNA quantification kits,
p. 361

Vericheck ddPCR
Mycoplasma detection kit,p. 363ddPCR supermix for residual
DNA quantification, p. 351

Vericheck ddPCR HEK293
res DNA quant kit, p. 361

ddPCR cell and gene therapy
assays, p. 357

C1000 Touch thermal cycler,p. 365

PrecisionAb validated
western blotting antibodies,p. 273
Antibodies, p. 271

Flow cytometry antibodies,pp. 272, 281
StarBright Dye labeled
antibodies, p. 276

Process-scalechromatography resins,
pp. 172, 173

ddPCR enhancer reagent A,
p. 363

bio-rad.com

Workflows for Translational Research | ix

Protein Immunotherapy Development

1

2

IMMUNE CELL ANALYSISIMMUNE PROTEIN MONITORING

To fully understand the effects of an
immunotherapy, researchers must often monitor
multiple pathways and cell types, which requires
analytical tools that support multiparameter
experiments. The fast and versatile ZE5 cell
analyzer can measure up to 30 parameters per
sample and can process a full 96-well plate inless than 15 minutes. You can easily assess thefull range of effects of your therapeutic using
Bio-Rad's extensive catalog of premade
conventional and custom human recombinant
antibodies.

Gaining insight into the roles proteins, such as
cytokines, play in the immune system drives thedevelopment of the most effective treatments.
Monitoring these proteins with tools that offer
multiplexing, accuracy, and specificity allows you
to pursue complete answers. Bio-Plex assays
and systems can analyze many cytokines andother critical proteins in a single experiment.
Design a custom Bio-Plex assay panel foryour unique needs, or save time with premade
panels. For even more flexibility, browse Bio-Rad’s
antibody library, including custom humanrecombinant antibodies.

ZE5 cell analyzer,
p. 45

Antibodies,p. 271

Related Products
HuCAL antibodies, p. 272
Cell health products,
pp. 56, 70
StarBright Dye labeled
antibodies, p. 276
Flow cytometry antibodies,pp. 272, 281

x | Workflows for Translational Research

Bio-Plex Pro Human
Immunotherapy 20-Plex panel,p. 301

Antibodies,p. 271

Related Products
ReadiLink antibody labelingkits, p. 52
Pioneer antibody discovery
platform, p. 279

Bio-Plex assays, p. 299
Stain-Free western blotting
workflow, p. 232
HuCAL antibodies, p. 272
StarBright Dye labeled
antibodies, p. 276
PrecisionAb validated
western blotting antibodies,p. 273

ReadyPrep protein extraction
kit, p. 2
TidyBlot reagent, p. 280
Clarity and Clarity Max ECL
western blotting substrates,
p. 248EveryBlot blocking buffer,
p. 245

bio-rad.com

Protein Immunotherapy Development

3

4

GENOMIC ANALYSIS AND
BIOMARKER DISCOVERYMANUFACTURING ANDQUALITY CONTROL

Genomics plays an important role in development,
testing, and monitoring of immunotherapeutics.
The CFX Opus real-time PCR system can beused with custom-built or predesigned PrimePCR
assays to measure multiple genes from many
samples at once. If absolute quantification isdesired, Droplet Digital PCR provides a solution
without the need for a standard curve.

For therapeutics built using novel scaffolds orwith modifications, identifying the best resin may
be complex. Bio-Rad’s chromatography resins
are designed for a wide range of applications
and are available in multiple formats to take youfrom development to commercial production.
Testing purity as manufacturing is scaled up
requires trusted, compliance-friendly tools.
The GS-900 calibrated densitometer delivers
protein-based purification electrophoresis data
within a regulatory framework with IQ/OQ and
U.S. FDA 21 CFR Part 11 compliant software.

CFX Opus real-time
PCR system,p. 370

QX600 Droplet Digital
PCR system,
p. 342

Related Products
PrimePCR assays, p. 376
ddPCR multiplex mutation
screening kits, p. 359
ddPCR copy number
determination assays,p. 356
PX1 plate sealer, p. 400
SingleShot cell lysis RT-qPCR
kits, p. 389

bio-rad.com

Resins,pp. 85, 172

GS-900 calibrated
densitometer,p. 288Related Products
Reliance Select cDNA
synthesis kit, p. xix, 394

Foresight chromatography
resin screening tools, p. 173

QX200 Droplet Digital PCR
system, p. 345

Reliance one-step multiplex
RT-qPCR supermix, p. 385

Process chromatography
resins, pp. 172, 173

SsoAdvanced universal
supermixes, pp. 380, 381

Foresight Pro columns,
pp. 132, 175

Vericheck ddPCR residual
DNA quantification kits,
p. 361

QX600 Droplet Digital PCR
system, p. 342

QX ONE Droplet Digital PCR
system, p. 344

Image Lab software, p. 289

Workflows for Translational Research | xi

Biosimilar Development

1

2

PURIFICATION

IMPURITIES

Biosimilar developers are challenged withproducing a product at a cost lower than thatof the original biologic while sourcing a reliable
stream of workflow components. Bio-Rad's
NGC chromatography systems and selectionof chromatography resins enable fast and easy
screening of methods and components when
designing a purification strategy for biosimilars.
NGC chromatography systems allow addition of
new capabilities as you move from discovery toprocess development. Expedite your analyses
using Stain-Free technology to visualize
biomolecules in your gels and confirm proteintransfer during western blotting.

To ensure the safety and efficacy of abiopharmaceutical, contaminants must be
identified and removed. Bio-Rad offers marketleading technologies for sensitive and robust
detection of host cell DNA and proteins. The
QX ONE Droplet Digital PCR system provides
maximum sensitivity and absolute quantificationof host cell DNA without the need for a DNA
extraction step, all in an automated system. For
the detection of protein contaminants, western
blotting is faster and more flexible than everbefore with the Stain-Free western blotting
workflow solutions.

NGC chromatographysystems,
p. 150Chromatography resinsand columns,
pp. 85, 172, 173

Related Products

QX ONE Droplet Digital
PCR system,
p. 344

Related Products

Foresight chromatography
resin screening tools, p. 173

StarBright Dye labeled
antibodies, p. 276

ddPCR supermix for residual
DNA quantification, p. 351

Process chromatography
resins, pp. 172, 173

ChromLab software, p. 159

Foresight Pro columns,
pp. 132, 175

Stain-Free gels,pp.196, 203

Vericheck ddPCR
Mycoplasma detection kit,
pp. 363

ChemiDoc imaging systems,
p. 285

Image Lab software, p. 289

EconoFit columns, p. 127

Vericheck ddPCR HEK293
res DNA size kit, p. 362

Antibodies, p. 271

Vericheck ddPCR HEK293
res DNA quant kit, p. 361

PrecisionAb validated
western blotting antibodies,p. 273

StarBright Dye labeled
antibodies, p. 276

xii | Workflows for Translational Research

Vericheck ddPCR residual
DNA quantification kits,
p. 361

Antibodies, p. 271

PrecisionAb validated
western blotting antibodies,p. 273
HuCAL antibodies, p. 272
Stain-Free western blotting
workflow, p. 232
ChemiDoc imaging systems,
p. 285
Trans-Blot Turbo transfer
system, p. 235
Membranes, p. 242

bio-rad.com

Biosimilar Development

3

4

COMPARABILITY

CHARACTERIZATION

Biosimilars must show comparability to their
originator biologic across multiple factors,
including structure, function, and efficacy.
Designing a cell-based assay to measurecomparability requires an understanding of theunderlying mechanism of action of the originatorand the biosimilar. The ZE5 cell analyzer
enables analysis of up to 30 parameters from asingle sample so that you can monitor multiple
cell types and lineages at once. Then easily
image cells right at your bench with the ZOE
fluorescent cell imager.

The biosimilar must be compared to thereference product to ensure that there are noclinically meaningful differences in terms ofsafety, efficacy, and immunogenicity. Reagent
quality in this testing is key, because results
form part of the totality of evidence required byregulatory agencies. Bio-Rad’s antibiotherapeutic
antibodies are developed using a guided
selection strategy enabling the generationof fully human, highly specific, inhibitory andnoninhibitory anti-idiotypic antibodies andspecialized drug-target complex binders.

ZE5 cell analyzer,
p. 45

ZOE fluorescent cell imager,
p. 80

Related Products
Bio-Plex 200 system, p. 293
Bio-Plex assays, p. 299
CFX Opus real-time PCRsystem, p. 370
PrimePCR assays, p. 376

HuCAL antibodies,p. 272Anti-idiotypic antibodies,p. 271

Related Products
ReadiLink antibody labelingkits, p. 52
Flow cytometry antibodies,pp. 271, 281

Stain-Free western blotting
workflow, p. 232

Bio-Plex 200 system, p. 293
Bio-Plex assays, p. 299

Antibodies, p. 271

PX1 plate sealer, p. 400

bio-rad.com

Workflows for Translational Research | xiii

Biomarker Discovery

1

2

CELLULARPROTEOMIC

The ability to uncover novel and rare cell
types through multiparameter analysis allows
researchers to characterize cell populations
in greater detail and identify subpopulations
that can serve as markers for disease. With theZE5 cell analyzer, up to 30 parameters can beanalyzed simultaneously to distinguish subtly
different cell populations. Reliably isolate rarecells with the Genesis cell isolation system, andperform traditional immunocytochemistry andhistochemistry studies for biomarkers in live or
fixed cells with the benchtop ZOE fluorescentcell imager.

Interrogating the proteomic profile of samples
can identify a myriad of potential biomarkers.
The Stain-Free western blotting workflow is avalidated and streamlined process that allows fast
and accurate quantitation of protein expression.
Antibody selection and specificity are also crucial
to the performance of your proteomic assays. For
newly identified biomarkers, Bio-Rad’s HuCAL
custom antibody technology can generate highly
specific custom human antibodies in only 8 weeks.
For plate-based analysis, the Bio-Plex 200 system
can be used with predesigned or customized
biomarker assay panels for multiplexed
protein analysis.

ZE5 cell analyzer,
p. 45

Genesis system,p. 36

Related Products
Antibodies, p. 271
ReadiLink antibody labelingkits, p. 52
StarBright Dye labeled
antibodies, p. 276

xiv | Workflows for Translational Research

Stain-Free western blotting
workflow, p. 232

Bio-Plex Pro Human
Immunotherapy 20-Plexpanel, p. 301

Related Products
ZOE fluorescent cell imager,
p. 80

Bio-Plex assays, p. 299

Genesis system, p. 36

StarBright Dye labeled
antibodies, p. 276

Celselect Slide technology,
p. 37

Antibodies, p. 271

PrecisionAb validated
western blotting antibodies,p. 273

ReadyPrep protein extraction
kits, p. 2
TidyBlot reagent, p. 280
Clarity and Clarity Max ECL
western blotting substrates,
p. 248
HuCAL antibodies, p. 272

bio-rad.com

Biomarker Discovery

3

4

GENOMICSOLUTIONS BEYOND DISCOVERY

Gene expression levels and the presence ofspecific genetic sequences are used as biomarkers
for many diseases. Bio-Rad provides powerfultools for analyzing gene expression, including
the QX600 Droplet Digital PCR system and the
CFX Opus real-time PCR system. CFX real-timesystems provide top performance, usability, andease of data acquisition and analysis. Reduce
hands-on time by pairing predesigned PrimePCR
panels available for over 100 pathways. For
applications challenged with low expression levels
or small sample sizes, ddPCR provides a powerfulsolution with accuracy and precision and makes
advanced multiplexing simple and robust.

Each biomarker’s proposed downstream
application defines the extent to which it must be
validated before it can move forward. Whatever
your validation pathway or downstream need,
you must have access to a reliable stream ofassay components and quality controls. Bio-Rad
partners with you on your biomarker journey,
providing solutions from early discovery to theclinic and beyond, and meeting your needs forsecurity of supply for critical materials.

QX600 Droplet Digital
PCR system,
p. 342

CFX Opus real-time
PCR system,p. 370Related Products
SsoAdvanced universal
supermixes, pp. 380, 381

Droplet Digital PCR assays,p. 356

SEQuoia Complete stranded
RNA library prep kit, p. 20

PrimePCR panels, p. 376

SEQuoia dual-indexed primer,
p. 22

Reliance Select cDNA
synthesis kit, p. 394
Reliance one-step multiplex
RT-qPCR supermix, p. 385

bio-rad.com

HuCAL antibodies,p. 272

Related Products

QX200 Droplet Digital PCR
system, p. 345

SingleShot cell lysis RT-qPCR
kits, p. 389

Bio-Plex 200 system,p. 293

SEQuoia RiboDepletion kit,
p. 21

Bio-Plex assays, p. 299
ddPCR multiplex mutation
screening kits, p. 359
Quality control products,
bio-rad.com/translationalQC
Diagnostic antibodies,bio-rad.com/translationalDA

SEQuoia Express stranded
RNA library prep kit, p. 21
QX600 Droplet Digital PCR
system, p. 342

Workflows for Translational Research | xv

COVID-19 Solutions
Bio-Rad continues to support the worldwide effort to fight severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), the virus associated with coronavirus disease 2019 (COVID-19),
by developing the needed tools for diagnosis, surveillance, and vaccine efficacy.

IgM

RNA
IgG
IgA

Active Infection
Molecular Test

Postinfection
Antigen Test

Serology Test

Diagnosis and Surveillance
Bio-Rad testing solutions for SARS-CoV-2 span the full course of the viral infection stages,
with options from active infection testing to postinfection antibody testing. Some products
have limited regional availability.
In addition, Bio-Rad provides researchers the tools needed to detect SARS-CoV-2 inwastewater, study population seroprevalence, and characterize how new SARS-CoV-2
variants spread over time. Simultaneous profiling of antibody response against SARS-CoV-2
nucleocapsid, receptor binding domain, spike 1, and spike 2 viral antigens can be useful inseroprevalence studies.

Vaccine Efficacy
With SARS-CoV-2 infections still occurring, the virus continues to evolve into more infectious
and transmissible variants, such as the Delta variant. Therefore, researchers are consistently
assessing the efficacy of COVID-19 vaccines and therapeutics against emerging strains.
Measuring the ability of neutralizing antibodies to inhibit infection by the arising variants ofconcern is crucial in assessing the efficacy of COVID-19 vaccines and comparing effectivenessof naturally acquired or vaccine-induced antibody response in postmarket surveillance studies.
The multiplex neutralization antibody assays measure SARS-CoV-2 neutralizing antibodies
against two wild types and 11 variants of receptor binding domain and spike 1 protein subunits.

xvi | COVID-19 Solutions

Bio-Rad solutions for COVID-19 diagnosis, surveillance, and vaccine efficacy. Bio-Rad has launched a series of products, such as the Reliance
SARS-CoV-2 RT-PCR assay kits, the PREvalence ddPCR SARS-CoV-2 Wastewater Quantification kit, and the Bio-Plex Pro Human SARS-CoV-2 Variant
Neutralization Antibody 11-Plex panel, to assist researchers by providing the needed tools to investigate and combat the COVID-19 pandemic.

We'll solve it. Together.
Bio-Rad has joined countless healthcare professionals, diagnostic laboratories, andresearchers to help combat the COVID-19 global pandemic. We are proud to be your partner indelivering the tools needed to identify individuals exposed to SARS-CoV-2 while also providing
resources for research and surveillance.
Bio-Rad Solution
Reliance SARS-CoV-2 RT-PCR Assay Kits, p. 392
SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit, p. 353
PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, p. 357
PrimePCR SARS-CoV-2 Variant Assays for RT-qPCR, p. 393
Bio-Plex Pro Human IgA SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, p. 317
Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, p. 317
Bio-Plex Pro Human IgM SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, p. 317
Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody
11-Plex Panel, p. 316
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel,p. 316
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay
Developer Kit, p. 316

Diagnosis

Surveillance

VaccineEfficacy

✓
✓
✓
✓
✓
✓
✓
✓

✓
✓
✓
✓
✓
✓
✓

✓
✓

Visit bio-rad.com/COVID19 for more information.

COVID-19 Solutions | xvii

bio-rad.com

New Products
Next-Generation Sequencing
SEQuoia Express Stranded RNA Library Prep Kit
A high-performance RNA-Seq library prep kit that enables customers to
overcome the challenge of a laborious library prep workflow by providing a costeffective 3-hour, 3-tube, automation-friendly solution for constructing robust
RNA libraries that efficiently capture mRNA and long non-coding RNA transcripts(>200bp). Page 21.

24 rxn and 96 rxn Kits Available

Omnition Analysis Software
Omnition analysis software version 1.0.0 (ATAC module) is a streamlined analysis
pipeline that can QC, analyze, and report single-cell ATAC-Seq data generated
with the SureCell® ATAC-Seq assay and ddSEQ isolator–deployed combinatorial
indexing ATAC-seq assays. Page 24.

SureCell ATAC-Seq Kit andOmnition Analysis Software Workflow

Cell Counting, Enumeration, and Enrichment
Genesis Cell Isolation System
The Genesis system supports the Celselect Slide technology, which captures
circulating tumor cells (CTCs) and rare cells for either recovery or on-slide staining
for downstream imaging on an automated microscope. The Genesis system
is an automated instrument that supports method development or protocol
optimization for customized applications with the Protocol Builder software tool.
Page 36.
Genesis System

Celselect Slide Techology
Celselect Slides are used with the Genesis system to gently capture CTCs
and rare cells based on their size, utilizing microfluidics and 56,400 individual
microchambers. Captured cells can be stained for immunofluorescent applications
such as enumeration and FISH, or recovered for downstream analysis such as
next-generation sequencing (bulk or single-cell), digital PCR, FISH, flow cytometry,or cells cultured for further in vitro and in vivo analysis. After staining, the cells arecounted on an automated microscope. Page 37.

xviii | New Products

llustration of a Celselect Slide with a blood sample
showing capture of CTCs while smaller blood
components (<8 µm) flow through.

In the U.S., call toll free at 1-800-424-6723

New Products
bio-rad.com

Chromatography: Laboratory andProcess Separations
NGC High-Flow Kit
Tubing kit for operating NGC 100 chromatography systems at high flow rates.
Recommended for operation of NGC 100 chromatography systems at flow rates
greater than 40 ml/min. Page 155.

NGC High-Flow Tubing Kit

Antibodies
Pioneer Antibody Discovery Platform
Bio-Rad has developed the Pioneer antibody library, a highly curated humanantibody library for biotherapeutic antibody discovery. It is 1000 times bigger
than the normal human immune repertoire and is designed to offer maximum
functional diversity and affinity with excellent developability. The Pioneer antibody
discovery platform delivers a greater selection of candidates with diverse binding
activity, and hence a significantly improved opportunity to identify a superior
therapeutic antibody. Page 279.

Pioneer Antifbody Discovery Platform

DNA Amplification/PCR
Reliance Select cDNA Synthesis Kit
This reverse transcription kit contains a novel chimeric enzyme that offers superior
performance with degraded samples such as formalin-fixed paraffin-embedded(FFPE) samples, inhibitors, and targets with high GC content. The 10 min reaction
protocol quickly and easily completes cDNA synthesis. The kit’s tolerance tocommon PCR inhibitors allows consistent RNA quantification, even with inhibitor
carryover, resulting in higher reproducibility. The kit is thermostable at 55ºC, so
secondary structures can be synthesized. Page 394

Reliance cDNA Synthesis Kit

PTC Tempo Thermal Cycler
The PTC Tempo thermal cycler represents the next generation of conventional
PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performancethat delivers accurate results, the PTC Tempo thermal cycler offers the flexibility toscale with your laboratory. The design features a refreshed, intuitive user interface
and flexible connectivity options for streamlining protocol management.
A motorized lid enables automation, taking your PCR to the next level withmodern instrument control. Page 365

In the U.S., call toll free at 1-800-424-6723

The PTC Tempo Thermal Cycler

New Products | xix

New Products
bio-rad.com

DNA Amplification/PCR (continued)
QX600 Droplet Digital PCR System
The QX600 Droplet Digital PCR (ddPCR) system delivers the same best-in-classperformance you’ve come to expect from Bio-Rad’s ddPCR systems, with theaddition of more channels for multiplexing. The system provides precise andsensitive multiplexing of more targets per sample with accurate and reproducible
quantification. The QX600 ddPCR system includes the new QX600 droplet reader,enabling six-color detection for multiplexing and allowing the clear discriminationof multiple targets with assays that can port over from the QX200 ddPCR system.
Page 342.

QX600 Droplet Digital PCR System

QX Manager Software, Premium Edition
QX Manager software, Premium Edition is a powerful, user friendly software
package for the acquisition and analysis of your Droplet Digital PCR data.
QX Manager software, Premium Edition provides the necessary functionality
to run and analyze ddPCR experiments, including support for locked assay
protocols and traceability for 21 CFR Part 11 compliance. Page 347.

QX Designer
QX Designer software is a powerful companion utility to QX Manager software,
Premium Edition that gives users the ability to author Assay Protocol Files that canbe locked, versioned, and deployed for use with QX Manager software, Premium
Edition. QX Designer software gives Assay Protocol authors fine-grain control
over how plates must be configured and processed, and results calculated anddisplayed. Page 348.

Bio-Rad Explorer Educational Products
Virus Disease Detection and Transmission Kit
Put your students into the roles of pathologist and epidemiologist as they
investigate the spread of a novel virus strain through a restaurant. Based on
published virus transmission case studies, this activity challenges students to use
molecular biology techniques to analyze patient samples and combine their datawith patient information to determine the mechanism of disease spread.
Use one of four scenarios to engage students and highlight important detailsof viral biology, pathophysiology, molecular diagnostic techniques, andtransmission modes. Page 434.

xx | New Products

Virus Disease Detection and Transmission Kit

In the U.S., call toll free at 1-800-424-6723

Sample Preparation
Protein Sample Preparation
Protein Extraction
Protein Sample Cleanup
Protein Fractionation
Protein Depletion

2
2
4
5
7

Nucleic Acid Sample Preparation
RNA Isolation
DNA Isolation
DNA Cleanup

9
9
13
15

Protein Sample Preparation
Magnetic Beads and Racks for Immunoprecipitation

bio-rad.com/immunoprecipitation

Protein Sample Preparation
Protein Extraction
Protein extraction tools such as cell lysis and extraction kits, as well as mini grinders, are available for extracting proteinsfrom cultured cells and tissues.

ReadyPrep Protein Extraction Kit
The ReadyPrep protein extraction kit (total protein)provides a simple, rapid, and reproducible method for
preparation of total cellular protein extracts from a wide
variety of biological samples. Use of this kit generates
protein samples that can be applied directly to a variety
of methods, including IEF and 2-D gel electrophoresis.
For More Information
Web: bio-rad.com/proteinextraction

Ordering Information
Catalog #Description

1632086

ReadyPrep Protein Extraction Kit (Total Protein), 20 preps

2 | Sample Preparation

Price, U.S. $190.00In the U.S., call toll free at 1-800-424-6723

Protein Sample Preparation
bio-rad.com/proteinsampleprep

Protein Extraction

See Also

Kits for Cell Lysis

Protein assay kits:
page 28.

Bio-Plex Cell Lysis Kit
This cell lysis kit is used to prepare cell lysates for western
blot analysis. Its protein extraction procedure yields western
blotting results similar to those generated by routine cell
lysis and protein extraction protocols.
For More Information
Web: bio-rad.com/proteinextraction and /bioplexcelllysis
Download bulletins: 3033 and 3034

ReadyPrep Mini Grinders
For grinding small biological samples for high recovery ofproteins (and nucleic acids), each mini grinder includes a
1.5 ml grinding tube containing a grinding resin and amatching pestle. The grinding resin is a neutral abrasive
material made of high tensile strength microparticles that
do not bind to proteins or nucleic acids. ReadyPrep mini
grinders are disposable and are nuclease- and proteasefree. The mini grinder tubes fit conveniently in mostbenchtop centrifuges.
For More Information
Web: bio-rad.com/proteinextraction

Ordering Information
Catalog #Description

1632146

ReadyPrep Mini Grinders, includes 20 mini grinders, sufficient for twenty 100 mg extractions

Price, U.S. $125.00

Bio-Plex Cell Lysis Kits
171304011
Bio-Plex Cell Lysis Kit, 1 x 96-well, includes cell lysis and wash buffers, factor 1 and factor 2171304012
Bio-Plex Cell Lysis Kit, 10 x 96-well, includes cell lysis and wash buffers, factor 1 and factor 2

180.001,510.00

In the U.S., call toll free at 1-800-424-6723

Sample Preparation | 3

Protein Sample Preparation
Protein Sample Cleanup

bio-rad.com/proteinsampleprep

Protein Sample Cleanup
See Also
Bio-Spin gel
filtration columns:
page 16.
Micro Bio-Spingel filtration
columns: page 16.

General-purpose cleanup kits and columns are available for the removal of salts and other contaminants.
Protein Sample Cleanup Kit Selection Guide
Kit Type and			Catalog Number
Applications
ProcedurePreparation		
Time		

Number ofPreps

Salt Removal
ReadyPrep 2-D cleanup
kits (#1632130, #1632140)

Cleanup of protein samples for
1-D and 2-D applications

TCA-like precipitation to remove salts,<1 hr		detergents, lipids, phenolic compounds							Reduction of streaking on 2-D gels
Concentration of dilute samples

Micro Bio-Spin P-6
columns (see pages 16–17)

Removal of salts and other
Size exclusion chromatography
5 min		25, 100
contaminants				50

Removal of Other Contaminants
ReadyPrep 2-D cleanup
Reduction of streaking on 2-D gelskits (#1632130, #1632140)
Concentration of dilute samples
			TCA-like precipitation to remove
salts, detergents, lipids, phenolic
compounds<1 hr		

50

Reduction, then alkylation of sample<2 hr		to remove disulfide bonds and prevent		
their re-formation

100

Reduction and Alkylation
ReadyPrep reductionReduction of streaking on 2-D gelsalkylation kit (#1632090)
Improved resolution of basic proteins			
For More Information
Web: bio-rad.com/proteincleanup
Download bulletins: 2934 and 2961

Ordering Information
Catalog #Description

Price, U.S. $ReadyPrep Kits
1632130
ReadyPrep 2-D Cleanup Kit, 50 preps
1632140
ReadyPrep 2-D Cleanup Kit, 5 preps
ReadyPrep 2-D Starter Kit, includes protein sample and reagents sufficient to rehydrate, focus, and transfer
1632105
to second-dimension gels; six 17 cm, ten 11 cm, or sixteen 7 cm ReadyStrip IPG strips (ReadyStrip IPG
strips,* precast SDS-PAGE gels, and gel stains not included in kit)
1632090
ReadyPrep Reduction-Alkylation Kit, 100 preps
ReadyPrep Kit Components and Related Products
1632091
ReadyPrep Proteomics Grade Water, 500 ml
1632083
ReadyPrep 2-D Rehydration/Sample Buffer 1, 10 ml, 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris,
0.001% bromophenol blue
1632106
ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer, 10 ml, 8 M urea, 2% CHAPS, 50 mM DTT,
0.2% Bio-Lyte 3/10 ampholyte, 0.001% bromophenol blue
Reducing and Alkylating Agents
1610611
Dithiothreitol (DTT), 5 g
1632101
Tributylphosphine (TBP), 200 mM, 0.6 ml
1632109
Iodoacetamide, 30 g
* See the prepacked Bio-Spin and Micro Bio-Spin gel filtration columns on pages 16–17.

4 | Sample Preparation

315.00125.00260.00215.00
40.00
38.00
44.00

81.00
44.00
145.00In the U.S., call toll free at 1-800-424-6723

Protein Sample Preparation
Protein Fractionation

bio-rad.com/proteinsampleprep

Protein Fractionation
Fractionation kits reduce sample complexity, helping to identify low-abundance proteins. Fractionation kits can be
subdivided into three groups that fractionate based on differential solubility, cellular location of the proteins of interest,
and protein charge.

Fractionation by Solubility
Fractionation Using Differential Protein Solubility
The ReadyPrep sequential extraction kit and the ReadyPrep
protein extraction kit (soluble/insoluble) both reduce sample
complexity using differential solubilization. The two kits
can be used independently, or the rehydration/sample
buffer from the soluble/insoluble kit can be used with thesequential extraction kit to create a fourth fraction for evenbetter resolution.
■

 eadyPrep sequential extraction kit — enables theRisolation of 3 different fractions of increasing solubility.
These fractions are isolated sequentially, allowing thevisualization of proteins that might not otherwise be seen.
Increasing solubilization strength is provided through theuse of stronger detergents for each subsequent fraction■

 eadyPrep protein extraction kit (soluble/insoluble) —R
uses a single fractionation step

ReadyPrep Kit Components and Related Products
Individual ReadyPrep kit components and related products
such as reducing agents are also available.
For More Information
Web: bio-rad.com/proteinfractionation
Download bulletins: 2934 and 2961

Ordering Information
Catalog #Description

ReadyPrep Kits
1632100
ReadyPrep Sequential Extraction Kit, 15 preps
1632085
ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps
ReadyPrep Kit Components and Related Products
1632103
ReadyPrep Sequential Extraction Kit Reagent 2, 10 ml, 8 M urea, 4% CHAPS, 40 mM Trisbase, 0.2% Bio-Lyte 3/10 ampholyte
1632104
ReadyPrep Sequential Extraction Kit Reagent 3, 10 ml, 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3–10,
40 mM Tris base, 0.2% Bio-Lyte 3/10 ampholyte
1632083
ReadyPrep 2-D Rehydration/Sample Buffer 1

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $215.00235.00
36.00
36.00
38.00

Sample Preparation | 5

Protein Sample Preparation
Protein Fractionation

bio-rad.com/proteinsampleprep

Fractionation by Cellular Location
Fractionation by Cellular Location
ReadyPrep protein extraction kits facilitate fractionationof proteins from different cellular locations such as themembrane, nucleus, or cytoplasm.
■■ eadyPrep protein extraction kit (cytoplasmic/
R
nuclear) — prepares fractions enriched in cytoplasmic or
nuclear proteins from eukaryotic samples
 eadyPrep protein extraction kit (membrane I) —R
offers a quick and effective protocol for isolating mostmembrane proteins. It does not require ultracentrifugationor preparation of density gradients■■

 eadyPrep protein extraction kit (membrane II) —R
offers a protocol for isolating more complex
membrane proteins eadyPrep protein extraction kit (signal) —R
for isolating proteins involved in intracellular membranetrafficking and signaling pathways. These include GPIanchored proteins, caveolin and associated proteins,acylated tyrosine kinases, and G proteins

For More Information
Web: bio-rad.com/proteinfractionation
Download bulletins: 2934 and 2961

Ordering Information
Catalog #Description

Price, U.S. $ReadyPrep Kits
1632089
ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps
1632088
ReadyPrep Protein Extraction Kit (Membrane I), 50 preps
1632084
ReadyPrep Protein Extraction Kit (Membrane II), 10 preps
1632087
ReadyPrep Protein Extraction Kit (Signal), 50 preps

450.00450.00270.00230.00Related Products
1632083
ReadyPrep 2-D Rehydration/Sample Buffer 1
1632085
ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps

38.00
235.00

6 | Sample Preparation

In the U.S., call toll free at 1-800-424-6723

Protein Sample Preparation
Protein Depletion

bio-rad.com/proteinsampleprep

Protein Depletion
Complex samples often require depletion of high-abundance proteins to allow the detection of the low-abundance ones.
Bio-Rad offers two different methodologies for protein depletion — the ProteoMiner protein enrichment system(which utilizes hexapeptide libraries) and Aurum serum and Affi-Gel Blue products (which utilize resins).

ProteoMiner Protein Enrichment System
The ProteoMiner protein enrichment system is a novel
sample preparation tool for reducing the dynamic rangeof protein concentrations in complex biological samples.
The ProteoMiner system:■■■

Enriches and concentrates low-abundance proteins thatcannot be detected through traditional methods orks with a variety of sample types (serum, plasma, urine,Wbile, cell lysates, tissues, and platelets) and is not limited by
the species’ origin
 tilizes a combinatorial library of hexapeptides ratherUthan immunodepletion, minimizing dependence on
available antibodies and preventing the codepletion of
low-abundance proteinsProteoMiner Protein Enrichment Kits — these kits
can be used with a variety of biological samples andare compatible with all major downstream proteomics
applications. Small- and large-capacity kits are available
for processing two or ten samples.
Apply sample

ProteoMiner Sequential Elution Kits — these kits utilize
multiple elution reagents to sequentially elute proteinsbased on different properties.
ProteoMiner Small- and Large-Capacity Kits
ProteoMiner kits for protein enrichment are offered informats optimized for varying starting amounts ofsample protein.
■■Small-capacity kits — optimized for use withlimited sample material (minimum 10 mg of protein
is recommended)Large-capacity kits — optimized for use withsamples in which at least 50 mg of protein is available

For More Information
Web: bio-rad.com/proteominer
Download bulletins: 5632, 5635, and 5841

Wash

Elute

Wash

EluateProteins

ProteoMiner
beads

FlowthroughProteoMiner technology is based on the interaction of complex protein samples with a large, highly diverse library of hexapeptides boundto chromatographic supports. In theory, each unique hexapeptide binds to a unique protein sequence. Because the bead capacity limits binding
capacity, high-abundance proteins quickly saturate their ligands (red and yellow beads) and excess protein is washed out during the procedure. In
contrast, low-abundance proteins are concentrated on their specific ligands (pink and teal beads), thereby decreasing the dynamic range of proteins inthe sample. When analyzed in downstream applications, the number of low-abundance proteins detected is dramatically increased.

In the U.S., call toll free at 1-800-424-6723

Sample Preparation | 7

Protein Sample Preparation
Protein Depletion

bio-rad.com/proteinsampleprep

Ordering Information
Catalog #Description

1633006

ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, for processing 10 mg of total protein,includes 10 spin columns, wash buffer, elution reagents, collection tubes
ProteoMiner Protein Enrichment Large-Capacity Kit, 10 preps, for processing 50 mg of total protein,includes 10 spin columns, wash buffer, elution reagents, collection tubes
ProteoMiner Protein Enrichment Introductory Small-Capacity Kit, 2 preps, for processing 10 mg of total
protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
ProteoMiner Protein Enrichment Introductory Large-Capacity Kit, 2 preps, for processing 50 mg of total
protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes

16330071633008
1633009

Price, U.S. $665.00910.00215.00245.00ProteoMiner Sequential Elution Kits
1633010
ProteoMiner Sequential Elution Small-Capacity Kit, 10 preps, for processing 10 mg of total protein,includes 10 spin columns, wash buffer, 4 sequential elution reagents, collection tubes
1633011
ProteoMiner Sequential Elution Large-Capacity Kit, 10 preps, for processing 50 mg of total protein,includes 10 spin columns, wash buffer, 4 sequential elution reagents, collection tubes

805.001,050.00

ProteoMiner Kit Accessories
1633003
ProteoMiner Sequential Elution Reagents, 10 preps, includes reagents only (columns not included),
to be used with #1633006 or #1633007
1633012
ProteoMiner Beads, 1525 mg of dry beads, columns and reagents not provide

300.00

Aurum Kits and Columns
The Aurum Affi-Gel Blue and Aurum serum kits andcolumns use affinity chromatography to reduce albumin and
IgG, which improves analysis of lower abundance proteins.
These products utilize a quick and easy spin-column format
and provide eluted proteins ready for analysis.
For More Information
Web: bio-rad.com/proteindepletion
Download bulletin: 2823

BeforeAfterAlbuminLightchain
IgG

Heavychain
IgG

Albumin

Heavychain
IgG

Lightchain
IgG

Removal of albumin and IgG from serum using the Aurum serum
protein mini kit. Total protein (1.32 mg) was purified on an Aurum
serum protein mini column. Left, untreated serum; right, serum treatedwith the Aurum kit.

Ordering Information
Catalog #Description

7326701
7326708

Aurum Serum Protein Mini Kit, 10 pack, includes columns and buffers
Aurum Affi-Gel Blue Mini Columns, 25 pack

8 | Sample Preparation

Price, U.S. $525.00390.00In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Sample Preparation
RNA Isolation

bio-rad.com/nasampleprep

Nucleic Acid Sample Preparation
RNA Isolation
Bio-Rad offers a broad range of sample preparation products for RNA isolation. Scalable and high-throughput products
are available.

See Also

RNA Isolation Product Selection Guide

Real-time PCRsystems: page 369.

			Aurum Total RNA Kits		

		

Mini Kit

Fatty and Fibrous Tissue Kit

96 KitPureZOL RNA
Isolation Reagent

Format		

Mini column
filtration (vacuum or spin)

Mini column
filtration (vacuum or spin)

96-well plate
filtration (vacuum or spin)

Single solution organic
extraction

1 x 106
8 x 1082 x 107——1 x 107
2.4 x 109
3 x 107
100 mg
100 mgMaximum starting
material amounts			
Cultured cells
2 x 106
1 x 107
Bacterial cells
2.4 x 109
2.4 x 109
Yeast cells
3 x 1073 x 107
Hard animal tissue 20 mg
100 mgSoft to moderately 40 mg
100 mghard animal tissue
100 mg—

100 mgIsolation method
Silica membrane			Plant tissue

40 mg

Lysis with PureZOL reagent,
purification on silica membrane

Silica membrane

Organic extraction

Number of preps50

50

192 (2 x 96-well plate)

50 or 100 (1 ml/prep)

Number of washes

3

3

3

—

DNase I included*

Yes

Yes

Yes

No

DNase I digest time

15 min (animal tissue, 25 min)15 min10 min—Total preparation time** <50–80 min (with DNase I digest)<50–80 min (with DNase I digest)<60 min (with DNase I digest)<60 min

Binding capacity>100 µg>100 µg>40 µg—Maximum elution
volume80 µl (2 x 40 µl elutions)2 x 40 µl80 µl30–100 µl

Yield***
		
		
		
		
		
		
		
		
		
		
		

20–30 µg/2 x 106 NIH 3T3 cells
140–150 µg/1 x 107 293H cells
10 µg/1 x 106 NIH 3T3 cells
90–100 µg/100 mg brain
17 µg/1 x 106 HeLa cells
20–40 µg/2 x 106 HeLa cells
15–20 µg/40 mg brain
50–60 µg/100 mg breast
11 µg/2 x 107 S. cerevisiae cells
30–55 µg/20 mg thymus
80–90 µg/100 mg heart
5 µg/8 x 108 E. coli cells
15–30 µg/40 mg spleen
60–70 µg/100 mg skin
5 µg/8 x 108 B. cereus cells
8–15 µg/40 mg liver
50–60 µg/100 mg cartilage		
15–20 µg/40 mg kidney
20–30 µg/2.4 x 109 E. coli cells		
8–15 µg/40 mg lung
60–65 µg/3 x 107 S. cerevisiae cells		
15–30 µg/40 mg potato
90–100 µg/100 mg potato		
20–30 µg/3 x 107 S. cerevisiae cells 5–10 µg/100 mg Arabidopsis		
10–15 µg/50 mg A. niger		
35–55 µg/2.4 x 109 E. coli cells
35–55 µg/2.4 x 109 B. cereus cells			

1.37 µg/1 x 105 293H cells
1.15 µg/mg brain
1.37 µg/mg fat
1.2 µg/mg heart
0.66 µg/mg cartilage
1.2 µg/mg skin
1.0 µg/mg potato
0.1 µg/mg Arabidopsis
0.38 µg/mg A. niger
0.63 µg/2.4 x 107 E. coli cells
0.31 µg/3 x 105
S. cerevisiae cells

PurityA260 /A280 of 1.9–2.1

A 260 /A 280 of 1.9–2.1

A260 /A280 of 1.9–2.1

A 260 /A 280 of 1.9–2.1

PCR reagents:
page 378.

DNA amplification/PCR:
page 341.

*			Removal not required.
**		Total preparation time will vary depending on the tissue or cell type and on which format is used (vacuum or spin).
*** Yields will vary depending on the developmental stage, tissue type or cell line, and growth conditions used.

In the U.S., call toll free at 1-800-424-6723

Sample Preparation | 9

Nucleic Acid Sample Preparation
RNA Isolation

See Also
PureZOL reagent:
page 11.
Lipid transfection
reagents:
page 329.
DNA amplification/PCR:
page 341.

bio-rad.com/nasampleprep

Aurum Total RNA Kits
Aurum total RNA kits are designed and formulated toassist in the isolation of highly pure and intact RNA from avariety of starting materials. Isolated RNA is compatible withdownstream applications including real-time qPCR, northernblotting, microarray analysis, and cDNA library construction.
■■■

Isolate high yields of RNA from a wide range of sample
types in less than 60 min
DNase treatment ensures genomic DNA removal
Produce ready-to-use RNA suitable for the mostdemanding downstream applications

Aurum total RNA kits offer streamlined processing using a
choice of vacuum- or spin-mediated protocols. If vacuummediated purification is preferred, the protocol may be
performed with a vacuum manifold.*
For More Information
Web: bio-rad.com/rna-isolation
Download bulletins: 2919, 2920, and 5282

Aurum Total RNA Mini Kit
The Aurum total RNA mini kit produces DNA-free total
RNA from a wide range of starting materials, including
cultured cells, bacteria, and yeast, as well as animal andplant tissues. The kit utilizes a stringent reagent composedof guanidine isothiocyanate and b-mercaptoethanol
for efficient sample lysis and quick RNase inactivation,
followed by purification on silica membrane in a spincolumn format using a spin- or vacuum-mediated protocol.
The kit can also be used for RNA cleanup and desalting.

Aurum Total RNA Fatty and Fibrous Tissue Kit

Aurum Total RNA 96 Kit
The Aurum total RNA 96 kit reproducibly isolates DNA-free
total RNA from cultured cells, bacteria, and yeast in lessthan 60 minutes in a 96-well format for high-throughput
total RNA isolation. The total RNA binding plate is designed
for use on the Aurum vacuum manifold.*
Consumables and Accessories
The reagents and plasticware used in the Aurum RNA
kits are available as refill orders. These products can beordered separately from the Aurum total RNA kits.

Aurum Total RNA Fatty and Fibrous Tissue Kit
The Aurum total RNA fatty and fibrous tissue kit isolates
total RNA from samples that are difficult to disrupt. The
kit is effective for purifications involving fatty and fibrous
tissues or samples, such as fungi, that are rich in RNases.
The kit utilizes PureZOL RNA reagent, a potent phenolbased reagent, which effectively lyses tissues and cells.
The kit includes spin columns that can be processed using
a choice of vacuum- or spin-mediated protocols.
* The Aurum vacuum manifold is no longer available. Please contact
Bio-Rad Technical Support for information on alternative products.

10 | Sample Preparation

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Sample Preparation
RNA Isolation

bio-rad.com/nasampleprep

Ordering Information
Catalog #Description

7326820

Aurum Total RNA Mini Kit, 50 preps, includes 50 RNA binding columns, 50 capless collection tubes(2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped sample tubes (1.5 ml), 1 vial lyophilized DNase I,
RNase-free reagents
Aurum Total RNA Fatty and Fibrous Tissue Kit, 50 preps, includes #7326870, 50 ml PureZOL RNA
isolation reagent
Aurum Total RNA Fatty and Fibrous Tissue Module, 50 preps, includes 50 RNA binding columns,
50 capless collection tubes (2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped sample tubes (1.5 ml),
1 vial lyophilized DNase I, RNase-free reagents, and plasticware
Aurum Total RNA 96 Kit, 2 x 96-well preps, includes 2 grow blocks, sealing tape, 2 RNA binding plates,
2 collection microplates, 2 vials lyophilized DNase I, RNase-free reagents

73268307326870*7326800

Price, U.S. $Aurum Consumables and Accessories
7326828
DNase I, RNase-free, lyophilized, 1 vial
7326802
Aurum Total RNA Lysis Solution, RNase-free, 85 ml
7326803
Aurum Total RNA Wash High-Stringency Solution, RNase-free, 150 ml
7326804
Aurum Total RNA Wash Low-Stringency Solution, 60 ml
7326826
Aurum RNA Binding Mini Columns, 50

230.00355.00250.00580.00

57.00
52.00
40.00
31.00
30.00

* Does not include PureZOL RNA isolation reagent (see catalog #7326880 or #7326890 to order separately).

See Also

PureZOL RNA Isolation Reagent

Aurum total RNA kits:
page 10.

The PureZOL RNA isolation reagent protocol is an
improvement over the rapid, widely used, and provenmethod of RNA isolation developed by Chomczynski and
Sacchi (1987). PureZOL RNA isolation reagent is a potent
monophasic combination of phenol and the chaotropic agent
guanidine isothiocyanate, which effectively lyses cells andtissues, deproteinates RNA, and inactivates endogenous
nucleases in a single step. DNA and protein are efficiently
removed from the RNA following phase separation.
The ready-to-use PureZOL RNA isolation reagent is a versatileand efficient means of isolating high yields of RNA from avariety of sources including cultured cells, animal and planttissue, yeast, virus, and bacteria samples. The single-solution
format permits recovery of RNA from small quantities oftissues or cells, making it ideally suited for gene expression
analysis or whenever sample quantities are limited. Total
RNA isolated using PureZOL RNA isolation reagent is free of

DNA and protein and can be used for northern blot analysis,in vitro translation, poly(A)+ selection, RNase protection
assays, RT-PCR, and molecular cloning. Since no spincolumns are used in the protocol, the protocol is scalable toaccommodate a wide range of sample sizes.
For More Information
Web: bio-rad.com/purezol

Ordering Information
Catalog #Description

73268807326890

PureZOL RNA Isolation Reagent, 50 ml
PureZOL RNA Isolation Reagent, 100 ml

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $110.00190.00Sample Preparation | 11

Nucleic Acid Sample Preparation
RNA Isolation

bio-rad.com/nasampleprep

iScript RT-qPCR Sample Preparation Reagent

■

■

■

103

102

101

Rapid protocol (5–10 min) efficiently stabilizes RNA0 ensitive detection of high-, medium-, and low-copySnumber gene targets directly from cell lysates

10

20

30

40

CyclesiScript RT-qPCR sample preparation reagent provides rapid
yet sensitive gene expression results for high-, medium-, andlow-copy number gene targets. HeLa cells were treated with
iScript RT-qPCR sample preparation reagent at 125 cells/μl and theexpression levels of three different genes were assessed. 18S rRNA(—), b-tubulin (—), and CMYC (—) expression levels were determinedby performing reverse transcription (cDNA synthesis kit) and qPCR
(iQ SYBR® Green supermix) directly from cell lysate preparations.
RFU, relative fluorescence units.

 eagent enables multiplex qPCR detection of up toR
4 targets from as few as 10 cells

For More Information
Web: bio-rad.com/iscript-sampleprep
Request or download bulletin: 5736

Cells

104

RFU

iScript RT-qPCR sample preparation reagent enables
efficient cell lysis and RNA stabilization for sensitive
quantitative gene expression analysis without RNA
purification. This unique reagent accelerates and streamlines
RT-qPCR analysis of cultured cells by eliminating the need topurify RNA. Reverse transcription and real-time PCR (qPCR)
can be performed directly from cell lysates. This reagent isideal for rapid, high-throughput gene expression analysis
such as validation of siRNA-mediated gene knockdown.

Spin

Collect for
RT-qPCR

10 minBind tocolumnmatrixDNase
treatment

Elute for
RT-qPCR

iScript RT-qPCR sample
preparation reagent method:
10 minLysis>30 minStandard method:>30 min

Ordering Information
Catalog #Description

1708898iScript RT-qPCR Sample Preparation Reagent, 100 reactions, 10 ml, contains RNase inhibitors andRNA stabilizers
iScript RT-qPCR Sample Preparation Reagent, 500 reactions, 5 x 10 ml, contains RNase inhibitors andRNA stabilizers

1708899

12 | Sample Preparation

Price, U.S. $150.00530.00In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Sample Preparation
DNA Isolation

bio-rad.com/nasampleprep

DNA Isolation
DNA Isolation Kit Selection Guide
		
Kit

DNA
Yield

Preparation
Time

Bacterial Culture
VolumePurification
FormatBindingMatrixGrowthMedia

Quantum Prep
≤40 µg<15 min
1–10 mlSpin
plasmid miniprep					DiatomaceousearthEnrichedor standard

Quantum Prep
≤400 µg
45 min20–40 mlSpin
plasmid midiprep					DiatomaceousearthEnrichedor standard

	Aurum≤20 µg<10 min
1–5 mlplasmid mini				

SilicamembraneStandard

Vacuumand spin

Quantum Prep Plasmid Kits
Quantum Prep Plasmid Miniprep Kit
The Quantum Prep miniprep kit offers high quality andyield in an easy spin-based procedure that takes less than
15 minutes from cell culture to purified plasmid. Plasmid
DNA is recovered in water or TE for immediate use in all
downstream molecular biology applications.
Quantum Prep Plasmid Midiprep Kit
The Quantum Prep plasmid midiprep kit uses a simple
spin-column procedure that significantly reduces the timerequired to generate the large quantities of plasmid DNA
needed to support applications such as transfection,
subcloning, and other enzymatic manipulations.

Quantum Prep Plasmid Miniprep Kit

Quantum Prep Plasmid Midiprep Kit

For More Information
Web: bio-rad.com/quantum-prep
Download bulletin: 2325

Ordering Information
Catalog #Description

7326100 uantum Prep Plasmid Miniprep Kit, 100 preps, includes 25 ml cell resuspension solution, 25 ml cell lysis
Qsolution, 25 ml neutralization solution, 20 ml Quantum Prep matrix, 63 ml wash solution, 100 spin columns
Quantum Prep Plasmid Midiprep Kit, 20 preps, includes 110 ml cell resuspension solution, 110 ml cell lysis
solution, 110 ml neutralization solution, 20 ml Quantum Prep matrix, 125 ml wash solution, 20 midi spin columns

7326120In the U.S., call toll free at 1-800-424-6723

Price, U.S. $195.00190.00Sample Preparation | 13

Nucleic Acid Sample Preparation
DNA Isolation

See Also
Lipid transfection
reagents:
page 329.
DNA amplification/PCR:
page 341.

bio-rad.com/nasampleprep

Aurum Plasmid Mini Kit
The easy-to-use Aurum plasmid mini kit improves theefficiency and throughput of plasmid purifications with a simple
bind-wash-elute protocol using silica membranes, all in lessthan 10 minutes. Spin- and vacuum-mediated protocols areavailable. The purified plasmid DNA can be immediately usedin any downstream molecular biology application.
The Aurum plasmid mini kit delivers high yields ofreproducible plasmid DNA preparations for:
■

■

■

■

■

Automated fluorescence-based sequencing
Restriction digestion
Ligation and transformation
Transfection
PCR

For More Information
Web: bio-rad.com/aurum-plasmid
Download bulletin: 2664

Ordering Information
Catalog #Description

7326400

Aurum Plasmid Mini Kit, 100 preps, includes plasmid-binding mini columns, 100 capless collection tubes,
reagents, protocol overview

Price, U.S. $217.40InstaGene Matrix
InstaGene matrix is designed for rapid isolation of small
amounts of genomic DNA of sufficient purity for PCR in lessthan an hour. The specially formulated 6% w/v Chelex 100
resin adsorbs cell lysis products that interfere with PCR,
leaving genomic DNA template in the supernatant where it
is immediately available for PCR reactions.

For More Information
Web: bio-rad.com/instagene-matrix
Download bulletin: 2074

InstaGene Protocol

PCR-ready
Add bacterial colony to water.

Spin and remove supernatant.

Add InstaGene matrix and incubate at 56°C for 30 min.
Vortex and incubate at 100°C for 8 min.

Spin; use supernatant for PCR.

Ordering Information
Catalog #Description

7326030

InstaGene Matrix, 20 ml, sufficient for 100 extractions

14 | Sample Preparation

Price, U.S. $54.00

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Sample Preparation
DNA Cleanup

bio-rad.com/nasampleprep

See Also

Chelex 100 Molecular Biology Grade Resin
Nuclease and ligase inhibitor-free, this pipettable, smallscale resin ensures complete removal of PCR inhibitorsand metal ions.

Analytical andbiotechnology grade
Chelex 100 Resin:
pages 96, 98.

For More Information
Web: bio-rad.com/chelex-mol-bio
Download bulletins 3318, 3356

Ordering Information
Catalog #Description

1421253

Chelex 100 Resin, molecular biology grade, 50 g, sodium, 200–400 dry mesh, 75–150 µm wet beadPrice, U.S. $195.00DNA Cleanup
Bio-Rad offers a variety of sample preparation and cleanup columns; many are also suitable for DNA fragment purification.

See Also

Freeze ’N Squeeze DNA Gel Extraction Spin Columns
Freeze ’N Squeeze spin columns offer a filtration-based
purification method, which provides a quick and effective
alternative to chemical extraction and electroelution methods.
The Freeze ’N Squeeze method uses centrifugation to draw
50–23,000 bp DNA out of agarose gel slices that have beenquickly frozen and thawed. Features include:
■

■

■

1

2

3

4

5

6

ReadyAgarose
precast gel system:
page 260.

7

DNA ladders:page 267.

1,000 bp

No solutions to prepare — save time and avoid using
toxic chaotropic materials

700
600
500
400
300
200
100

DNA immediately available for PCR, subcloning, ligations,and sequencing reactions
Less than 1 min of hands-on time

Recovery of DNA
fragments using
Freeze ’N Squeeze
spin columns.
Extracted fragments
were rerun on a
ReadyAgarose 1% TAEgel. Lane 1, precision
molecular mass ruler;lane 7, EZ Load 100 bp
PCR molecular ruler.

For More Information
Web: bio-rad.com/freeze-n-squeeze
Freeze ’N Squeeze Method–20ºC for 5 min
Purified DNA

Cut out gel slice.

Chop into pieces.

Transfer to spin column
and freeze for 5 min.

Spin for 3 min.

Recover purified DNA.

Ordering Information
Catalog #Description

73261657326166 reeze ’N Squeeze DNA Gel Extraction Spin Columns, 25 columns
F
Freeze ’N Squeeze DNA Gel Extraction Spin Columns, 100 columns

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $60.00
185.00Sample Preparation | 15

Nucleic Acid Sample Preparation
DNA Cleanup

bio-rad.com/nasampleprep

Prepacked Spin Columns
Prepacked size exclusion spin columns allow easycleanup and purification of DNA and proteins from lower
MW contaminants. Bio-Spin, Micro Bio-Spin, and
PCR Kleen columns clean up DNA or protein samples
quickly and easily using size exclusion chromatography.

These columns are available in multiple sizes and offer
multiple MW exclusion limits to accommodate a varietyof needs. Use the chart below to choose the column thatbest meets your needs.

Prepacked Spin Column Selection Guide			

Bio-Spin P-6

Micro Bio-Spin P-6

Bio-Spin P-30

Micro Bio-Spin P-30

PCR Kleen

Packed support			Special grade
Bio-Gel P-6 gelSpecial grade
Bio-Gel P-6 gelSpecial grade
Bio-Gel P-30 gel

Special grade
Bio-Gel P-30 gel

Special grade
size exclusion gel

Equilibration buffer			

10 mM Tris, pH 7.4, 10 mM Tris, pH 7.4,
or SSC buffer*
or SSC buffer*

10 mM Tris, pH 7.4, 10 mM Tris, pH 7.4,
or SSC buffer*
or SSC buffer*

10 mM Tris, 1 mM
EDTA, pH 7.0

•

•

—

—

—

		 Labeling reactions:		 removal of unincorporated
		 nucleotides >20 bases
		 or bp from DNA

—

—

•

•

—

		 Removal of primers and		 primer-dimers from
		 PCR products >200 bp

—

—

—

—

•

		 Buffer exchange		 (restriction fragments,
		 PCR products, enzyme
		 reactions, sequencing
		 templates)

•

•

—

•

—

		 DNA sequencing
		 reaction mixture cleanup**

—

—

•

•

—

Applications
		 Desalting of		 oligonucleotides
		 >20 bases

		 Riboprobe cleanup***

—

—

—

•

—

		 Desalting of antibody,		 enzyme, and protein
		 solutions

•

•

—

•

—

		 Purification of proteins		 of molecular weight		 >6,000

•

•

—

—

—

		 Purification of proteins		 of molecular weight
		 >40,000

—

—

•

•

—

Bed volume

1.1 ml

0.7 ml1.1 ml

0.7 ml

0.6 mlRetention and recovery
			
			
			

90% recovery of
20 bases or bp,
99% retentionof salts

90% recovery of
20 bases or bp,
99% retentionof salts

95% recovery of
22 bases or bp,
98% retentionof ddNTPs

95% recovery of
22 bases or bp,
98% retentionof ddNTPs

85% recovery of≥700 bp, 95%
retention of primers
and primer-dimers

Molecular weightexclusion limit,
globular proteins

6,000

6,000

40,00040,0008,000,000

25–100 µl

Sample volume

50–100 µl

10–75 µl50–100 µl

10–75 µl

Centrifuge type

Swinging bucket

Microcentrifuge

Swinging bucket

Microcentrifuge

Microcentrifuge

Autoclavability

Yes

Yes

Yes

Yes

Yes

*		150 mM NaCl, 17.5 mM sodium citrate, pH 7.0.
**		In Tris buffer.
*** In RNase-free Tris buffer.

16 | Sample Preparation

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Sample Preparation
DNA Cleanup

bio-rad.com/nasampleprep

Prepacked Bio-Spin and Micro Bio-Spin Columns
Bio-Spin and Micro Bio-Spin columns clean up and remove
salts, nucleotides, dye terminators, and small molecules from
DNA, RNA, and protein samples in 10 minutes. Filled withspecially sized Bio-Gel P gels, these columns are shippedfully hydrated in Tris or SSC buffer. They yield 95% recovery
of DNA >22 bp and allow sample loads of 10–100 µl. For safe
riboprobe preparation use RNase-free Micro Bio-Spin P-30
Tris spin columns.

For More Information
Web: bio-rad.com/dna-cleanup
Download bulletin: 2311

End cap

Reservoir

Reservoir
3 cm
2 cm working bed height

5 cm
3.7 cm working bed height

See Also
Bio-Gel P resin:
page 117.
Empty Bio-Spin,
Micro Bio-Spin, andMini Bio-Spin columns:
page 140.

0.8 ml bed
volume

1.2 ml bed volume

For More Information
Web: bio-rad.com/dna-cleanup and /protein-cleanup

PCR Kleen Spin Columns
PCR Kleen columns are prepacked spin columns for
purifying PCR products and other DNA molecules >200 bp
directly from reaction mixtures. A simple 4-minute spin
effectively removes salts, nucleotides, enzymes, primers,
and primer-dimers. Purified DNA fragments are immediately
available for secondary PCR, subcloning, restriction digests,
ligations, sequencing, and other enzymatic manipulations.

End cap

Porous 30 µm
polyethylene bed
support retains
fine particles

Luer end
fitting withsnap-off tip

Luer end fittingwith snap-off tip
Prepacked Bio-Spin and Micro Bio-Spin Columns

PCR Kleen Spin Columns

Ordering Information
Catalog #Description

Price, U.S. $Micro Bio-Spin Columns with Bio-Gel P-6 in Tris Buffer
7326221Micro Bio-Spin P-6 Columns, includes 25 columns in Tris buffer, 50 collection tubes
7326222Micro Bio-Spin P-6 Columns, includes 100 columns in Tris buffer, 200 collection tubes

110.00365.00Micro Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer
7326223
Micro Bio-Spin P-30 Columns, includes 25 columns in Tris buffer, 50 collection tubes
7326224Micro Bio-Spin P-30 Columns, includes 100 columns in Tris buffer, 200 collection tubes
7326250Micro Bio-Spin P-30 Columns, includes 25 columns in Tris buffer, 50 collection tubes, RNase-free7326251
Micro Bio-Spin P-30 Columns, includes 100 columns in Tris buffer, 200 collection tubes, RNase-free

110.00365.00125.00440.00Micro Bio-Spin Columns with Bio-Gel P-6 in SSC Buffer
7326200
Micro Bio-Spin P-6 Columns, includes 25 columns in SSC buffer, 50 collection tubes
7326201
Micro Bio-Spin P-6 Columns, includes 100 columns in SSC buffer, 200 collection tubes

110.00365.00Micro Bio-Spin Columns with Bio-Gel P-30 in SSC Buffer
7326202
Micro Bio-Spin P-30 Columns, includes 25 columns in SSC buffer, 50 collection tubes

110.00

Bio-Spin Columns with Bio-Gel P-6 in Tris Buffer
7326227
Bio-Spin P-6 Columns, includes 25 columns in Tris buffer, 50 collection tubes
7326228
Bio-Spin P-6 Columns, includes 100 columns in Tris buffer, 200 collection tubes

135.00455.00

Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer
7326231
Bio-Spin P-30 Columns, includes 25 columns in Tris buffer, 50 collection tubes
7326232
Bio-Spin P-30 Columns, includes 100 columns in Tris buffer, 200 collection tubes

135.00455.00

Bio-Spin Columns with Bio-Gel P-6 in SSC Buffer
7326002
Bio-Spin P-6 Columns, includes 25 columns in SSC buffer, 50 collection tubes

155.00

Bio-Spin Columns with Bio-Gel P-30 in SSC Buffer
7326006
Bio-Spin P-30 Columns, includes 25 columns in SSC buffer, 50 collection tubes

155.00PCR Kleen Spin Columns
7326300
PCR Kleen Spin Columns, 25

78.75

In the U.S., call toll free at 1-800-424-6723

Sample Preparation | 17

Sample Preparation
Subject Index

Subject Index
Aurum Kits and Columns, 8
Aurum Plasmid Mini Kit, 14
Aurum Total RNA Kits, 10
Chelex 100 Molecular Biology Grade Resin, 15
Fractionation by Cellular Location, 6
Fractionation by Solubility, 5Freeze ’N Squeeze DNA Gel Extraction Spin Columns, 15InstaGene Matrix, 14
iScript RT-qPCR Sample Preparation Reagent, 12Kits for Cell Lysis, 3
Prepacked Spin Columns, 16
ProteoMiner Protein Enrichment System, 7
PureZOL RNA Isolation Reagent, 11
Quantum Prep Plasmid Kits, 13
ReadyPrep Protein Extraction Kit, 2

18 | Sample Preparation

In the U.S., call toll free at 1-800-424-6723

Next-Generation Sequencing
Transcriptome Sequencing
SEQuoia RNA Library Prep Solutions
RNA-Seq Analysis Software

20
20
22

Single-Cell Sequencing

23

Transcriptome Sequencing
SEQuoia RNA Library Prep Solutions

bio-rad.com/ngs

Transcriptome Sequencing
SEQuoia RNA Library Prep Solutions
The latest advancement in RNA-Seq library preparation chemistry, SEQuoia RNA library prep solutions profile the wholetranscriptome with an easy and streamlined workflow. The unique patented enzymatic technology of SEQzyme is adopted
in two of the RNA library prep kits, one for capturing both long and short RNAs and the other for capturing mRNA andlong noncoding RNAs (>200 bp). Both kits are compatible with the SEQuoia RiboDepletion kit for removal of constructs
originating from ribosomal RNA, and SEQuoia dual indexed primers, which enable researchers to pool libraries to run themon a single flow cell, greatly reducing the overall sequencing cost.

SEQuoia Complete Stranded RNA Library Prep Kit
The SEQuoia Complete stranded RNA library prep kit is ahigh-performance kit for RNA-Seq that allows the captureof both long and short RNAs in a single library, even fromlimited and low-quality samples. Featuring SEQzyme,
a proprietary engineered enzyme that couples cDNA
synthesis with adaptor addition in a continuous synthesis
reaction, this kit constructs sensitive and diverse libraries
that are compatible with Illumina® sequencing platforms.
Benefits:
■

■

■

■

Captures both long and short RNA in a single library prep
 alidated with low-quality and degraded RNA samples
V(such as FFPE) nables a streamlined library construction in less thanE
4 hours
Includes integrated bioinformatics solution

For More Information
Web: bio-rad.com/SEQuoiaComplete
Download bulletins: 7273 and 3268

Ordering Information
Catalog #Description

17005726
17005710

SEQuoia Complete Stranded RNA Library Prep Kit, 24 reactions
SEQuoia Complete Stranded RNA Library Prep Kit, 96 reactions

20 | Next-Generation Sequencing

Price, U.S. $4,220.00
4,750.00

In the U.S., call toll free at 1-800-424-6723

Transcriptome Sequencing
SEQuoia RNA Library Prep Solutions

bio-rad.com/ngs

New SEQuoia Express Stranded RNA Library Prep Kit
The SEQuoia Express stranded RNA library prep kit
provides a faster and less complicated library preparation
protocol for capturing long RNA biotypes (messenger
RNA and long noncoding RNA). Featuring a 3-tube kit
configuration and short 3-hour protocol compatible with
automation, the kit affords high capture efficiency andlow sequencing bias from RNA-Seq performed in various
sample types and across a broad dynamic range ofsample input.
Benefits:
■

■

■

■

Simplified workflow can be completed in under 3 hours

For More Information
Web: bio-rad.com/SEQuoiaExpress
Download bulletin: 3330

 onvenient 3-tube configuration that minimizes pipettingC
steps and hands-on time
 fficient and unbiased capture of mRNA and longEnoncoding RNA
Integrated bioinformatics solutions

Ordering Information
Catalog #Description

17006487

SEQuoia Ribodepletion Kit, 24 reactions

Price, U.S. $4,220.00

SEQuoia RiboDepletion Kit
The SEQuoia RiboDepletion kit eliminates RNA-Seq libraryfragments that originated from cytoplasmic ribosomal
RNA (rRNA) and mitochondrial ribosomal RNA (mt rRNA).
With innovative post-library depletion technology, the kit
is ideal for researchers working with a limited sample,
targeting rare transcripts, or generating sequencing datathat represent the complete transcriptome. Furthermore,
multiple libraries can be pooled and depleted in a single
reaction, maximizing depletion efficiency and saving
significant time and cost.

■

Benefits:■■

 liminate fragments derived from ribosomal andEmitochondrial RNA etain rare transcripts commonly lost in pre-libraryR
depletion methods■ ave time and money by enabling pooled depletion ofS
multiple libraries
 tand-alone kit is compatible with most RNA-Seq libraryS
prep kits

For More Information
Web: bio-rad.com/SEQuoiaRiboDepletion
Download bulletin: 7460

Ordering Information
Catalog #Description

12017297
12017265

SEQuoia Express Stranded RNA Library Pre Kit, 24 reactions
SEQuoia Express Stranded RNA Library Pre Kit, 96 reactions

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $4,220.00

Next-Generation Sequencing | 21

Transcriptome Sequencing
SEQuoia RNA Library Prep Solutions

bio-rad.com/ngs

SEQuoia Dual-Indexed Primer Kit
SEQuoia dual indexed primers are high-quality, ready-touse i5 and i7 indexed primer pairs designed for use with
the SEQuoia Complete stranded RNA library prep kit andthe SEQuoia Express stranded RNA library prep kit toconstruct libraries for multiplex sequencing using Illumina®
platforms. The nucleotide sequence of each primer
set has been balanced to achieve optimal multiplexedsequencing results. These unique dual indexed primer
pairs are available in sets of 12 or 96.
For More Information
Web: bio-rad.com/SEQuoiaComplete

Ordering Information
Catalog #Description

12011928

SEQuoia Dual Indexed Primer Set, 12 unique dual indexed adaptors in 12 separate vials with sufficientvolume for eight reactions per vial (96 total reactions)
SEQuoia Dual Indexed Primer Plate, 96 unique dual indexed adaptors plated along thecolumns in a 96-well PCR plate

12011930

Price, U.S. $4,220.00

RNA-Seq Analysis Software
SeqSense NGS Data Analysis Software for SEQuoia Products
SeqSense NGS Data Analysis software is for use with theSEQuoia Complete and Express stranded RNA library
prep kits. It is available in two options so you can work
online via a web application, or locally, using your own
infrastructure, through a Docker container. Both versions
use FASTQ files as input for secondary analysis, using thesame underlying pipeline, and can produce BAM files,
count matrices, and reports as output for tertiary analysis.
For More Information on the Docker Toolkit
Web: bio-rad.com/ngs/analysis
For More Information on the Web Application
Web: seqsense.bio-rad.com

22 | Next-Generation Sequencing

RNA-Seq Analysis Software Options
SEQuoia Docker
ToolkitFeatureSeqSense Analysis
Web Application

The SeqSense web
The Docker container
application allows you to(command line scripts
and libraries) allows you to view, manage, and analyze
view, manage, and analyze your FASTQ sample
files online
your FASTQ sample files
deployed locally, on yourinfrastructure

Reagent kits SEQuoia Complete stranded RNA library prep kit
supported
SEQuoia Express stranded RNA library prep kit

In the U.S., call toll free at 1-800-424-6723

Single-Cell Sequencing
Single-Cell Partitioning

bio-rad.com/ddSEQSingle-Cell Sequencing
The ddSEQ single-cell isolator is a droplet-based single-cell isolation system for high-throughput single-cell chromatin
accessibility studies.

ddSEQ Single-Cell Isolator
The ddSEQ single-cell isolator is part of the Bio-Rad
single-cell next-generation sequencing (NGS) solution,
which works with the SureCell® ATAC-Seq library prep kit.
The technology utilizes disposable microfluidic cartridges
to coencapsulate single cells or single nuclei andbarcoded beads into subnanoliter droplets, where cellbead pairing and barcoding occur. ATAC-Seq libraries aresubsequently prepared and sequenced, and analysis isconducted with the Bio-Rad ATAC-Seq analysis pipeline.
■■■Process hundreds to tens of thousands of cells per day ensitive and unbiased characterization of chromatin
S
accessibility and transcriptional signatures
 calable solution to accommodate a wide range ofS
experimental designs■■

Unbiased profiling of diverse cell populations
 n end-to-end single-cell sequencing solution with aAstreamlined analysis toolkit

For More Information
Web: bio-rad.com/ddSEQ
Download bulletins: 7273 and 3268

Ordering Information
Catalog #Description

12004336

ddSEQ Single-Cell Isolator, a droplet-based single cell isolation system for single-cell chromatin
accessibility studies; includes isolator, test cartridges, and cartridge holder

In the U.S., call toll free at 1-800-424-6723

Next-Generation Sequencing | 23

Single-Cell Sequencing
Single-Cell Epigenomics

bio-rad.com/scATACSeqKit

SureCell ATAC-Seq Library Prep Kit
The SureCell® ATAC-Seq library prep kit enables highly
sensitive genome-wide open chromatin profiling at thesingle-cell level. The kit leverages the throughput capabilitiesof the ddSEQ single-cell isolator to partition thousands ofnuclei or cells, each into its own nanoliter-sized droplet.
■

■

■

■

Greater than 65% and up to 95% cell capture efficiency
 ell throughput range of as few as 400 or more thanC
5,000 cells or nuclei per sample
 est sensitivity possible from the highest number ofB
unique sequencing fragments that map to the nuclear
genome and transcription start sites
 treamlined data QC, analysis, and reporting with theS
companion bioinformatics pipeline■

Runs on the ddSEQ single-cell isolator■

Works with nuclei or whole cells

For More Information
Web: bio-rad.com/scatac-seq
Request or download bulletin: 7167

Ordering Information
Catalog #Description

17004620

SureCell ATAC-Seq Library Prep Kit, includes ddSEQ M cartridges (12009359), SureCell ATAC-Seq
reagent box A (1200358), and SureCell ATAC-Seq reagent box B (12009357)

12009360

SureCell ATAC-Seq Index Kit, to be used as a part of the SureCell ATAC-Seq library prep kit

New Omnition Analysis Software

SureCell ATAC-Seq and Omnition Analysis Software Workflow

Omnition analysis software version 1.0.0 (ATAC module) is
a streamlined analysis software tool that enables quality
assessment, analysis, and reporting of single-cell ATACSeq data generated from the SureCell ATAC-Seq assay andddSEQ-isolator–deployed combinatorial indexing
ATAC-Seq assays.
Omnition analysis software’s superior usability andfunctionality provide:
■

■

■

■

Batch analysis and reporting for multiple samples
 upport for ddSEQ isolator–deployed combinatorial
S
indexing ATAC protocols
 pen-source download available atOgithub.com/BioRadOpenSource/omnition

For More Information
Web: bio-rad.com/omnition
Download Bulletins: 3408 and 3354

Streamlined, robust, and efficient workflow

24 | Next-Generation Sequencing

In the U.S., call toll free at 1-800-424-6723

Headline
bio-rad.com/omnition

Subject Index

Subject Index
ddSEQ Single-Cell Isolator, 23
Omnition Analysis Software, 24
SeqSense NGS Data Analysis Software for SEQuoia Products, 22
SEQuoia Complete Stranded RNA Library Prep Kit, 20
SEQuoia Dual-Indexed Primer Kit, 22
SEQuoia Express Stranded RNA Library Prep Kit, 21
SEQuoia RiboDepletion Kit, 21
SureCell ATAC-Seq Library Prep Kit, 24

In the U.S., call toll free at 1-800-424-6723

Next-Generation Sequencing | 25

26 | Next-Generation Sequencing

In the U.S., call toll free at 1-800-424-6723

Sample Quantitation
Protein Assay Kits

28

Cuvettes

31

Protein Assay Kits
bio-rad.com/proteinassays

Protein Assay Kits
See Also
Cuvettes: page 31­.

Videos
Product
Tutorial

Determining the concentration of protein samples is critical for many experiments, and most protein samples can bequantitated by a colorimetric assay. In a typical protein assay, a chemical reagent is added to a protein sample solution,
producing a color change that is measured with a spectrophotometer or microplate reader and compared to a standard
curve of known protein concentrations. Bio-Rad offers four protein assays, each with a unique set of advantages.
All assays can be easily automated for large numbers of samples.
Bio-Rad offers four protein assays:■■■

Quick Start Bradford protein assay — the single-stepmethod for determining protein concentration in solution
 io-Rad protein assay — for quantitating most proteinsBand polypeptides with MWs >3,000

bio-rad.com/bradford■

DC protein assay — for use with samples
containing detergent
RC DC protein assay — for complex sample solutions
containing reducing agents and detergents

For More Information
Web: bio-rad.com/proteinassays
Download bulletins: 1069, 6835, 6836, 6837, 6838, and 6852

Protein Assay Selection Guide*			Quick Start Bradford

Bio-RadDC

RC DC

Application
			Single-step method

Quantitates mostproteins

For samples withdetergent

For complex samples
with reductants and detergents

Method adapted from

Bradford (1976)

Bradford (1976)

Lowry et al. (1951)

Lowry et al. (1951)

Standard-concentration assay
Sample volume
Linear range

100 µl0.125–1.5 mg/ml

100 µl0.125–1.5 mg/ml

100 µl0.125–1.5 mg/ml

100 µl
0.2–1.5 mg/ml

Low-concentration assay
Sample volume
Linear range

1 ml
1.25–25 µg/ml

800 µl
1.25–25 µg/ml

200 µl5–250 µg/ml

200 µl5–250 µg/ml

Microplate assay sample volume

5 µl

10 µl

5 µl

**

Minimum incubation time

5 min

5 min

15 min15 minAssay wavelength

595 nm

595 nm650–750 nm

650–750 nm* Standard-concentration assay is designed for smaller sample volumes of higher protein concentration, while the low-concentration assay isdesigned for larger sample volumes of lower protein concentration.
** To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC protein assay instruction
manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate
assay protocol in the DC protein assay instruction manual can be followed.

Quick Start Bradford Protein Assay
The Quick Start Bradford protein assay is a streamlined,
accurate procedure for determining the concentrationof protein in solution. Ready-to-use dye reagent andprediluted protein standards allow one-step determinationof protein concentration.
Quick Start Bradford kits offer either bovine serum albuminor bovine g-globulin standard sets. Each kit contains twoaliquots each of seven concentrations (0.125, 0.25, 0.5, 0.75,
1.0, 1.5, and 2.0 mg/ml) conveniently packaged in screwcap
vials, eliminating ampoules and ensuring protein stability forone year when stored at 4°C. Standards are also available infive 2 mg/ml aliquots for creating your own dilutions.
The 1x dye reagent can be used for performing 1 and 5 ml
standard assays, microplate assays, or microassays.

28 | Sample Quantitation

For More Information
Web: bio-rad.com/quickstart
Download bulletins: 1069, 2969, 6835, and 6852

In the U.S., call toll free at 1-800-424-6723

Protein Assay Kits
bio-rad.com/proteinassays

Ordering Information
Catalog #Description

5000201

 uick Start Bradford Protein Assay Kit 1, includes 1x dye reagent (1 L), bovine serum albumin standardQ(5 x 2 mg/ml); sufficient for 200 standard assays or 4,000 microplate assays
Quick Start Bradford Protein Assay Kit 2, includes 1x dye reagent (1 L), bovine serum albumin standard set(2 sets of 7 concentration standards, 0.125–2.0 mg/ml, 2 ml)
Quick Start Bradford Protein Assay Kit 4, includes 1x dye reagent (1 L), bovine g-globulin standard set(2 sets of 7 concentration standards, 0.125–2.0 mg/ml, 2 ml)

5000202
5000204

Price, U.S. $150.00190.00195.00

Reagents
5000205

Quick Start Bradford 1x Dye Reagent, 1 L

115.00Accessories
5000206
5000207
5000208
5000209

Quick Start Bovine Serum Albumin Standard, 5 x 2 ml vials of 2 mg/ml
Quick Start Bovine Serum Albumin Standard Set, 2 sets of 7 concentration standards, 0.125–2.0 mg/ml
Quick Start Bovine g-Globulin Standard, 5 x 2 ml vials of 2 mg/ml
Quick Start Bovine g-Globulin Standard Set, 2 sets of 7 concentration standards, 0.125–2.0 mg/ml

79.00
140.0081.00
140.00

Bio-Rad Protein Assay
The Bio-Rad protein assay is a simple colorimetric assay
for determining total protein concentration. It is easy toadapt the assay from the standard-concentration range
to a low-concentration microassay or for use in 96-well
microplates for rapid determinations.
The Bio-Rad protein assay is based on the Bradford dyebinding procedure (Bradford 1976), which measures thecolor change of Coomassie Brilliant Blue G-250 dye when
it binds to protein (primarily to basic and aromatic aminoacid residues). The assay quantitates most proteins andpolypeptides with MWs >3,000. Some detergents andbasic buffers interfere with the assay.
For More Information
Web: bio-rad.com/BRprotein
Download bulletins: 1069, 1123, 6836, and 6852

Ordering Information
Catalog #Description

5000001 io-Rad Protein Assay Kit I, includes 450 ml dye reagent concentrate, bovine g-globulin standard;Bsufficient for 440 standard assays or 2,200 microplate assays
Bio-Rad Protein Assay Kit II, includes 450 ml dye reagent concentrate, bovine serum albumin standard;sufficient for 440 standard assays or 2,200 microplate assays

5000002

Price, U.S. $150.00150.00

Reagents
5000006

Bio-Rad Protein Assay Dye Reagent Concentrate, 450 ml

120.00Accessories
5000005
5000007

Protein Standard I, bovine g-globulin, reconstituted volume 20 ml
Protein Standard II, bovine serum albumin, reconstituted volume 20 ml

74.00
74.00

In the U.S., call toll free at 1-800-424-6723

Sample Quantitation | 29

Protein Assay Kits
bio-rad.com/proteinassays

DC Protein Assay
The DC (detergent compatible) protein assay is a colorimetricassay for protein determination of samples that contain
detergents. The reaction is similar to the well-documented
Lowry assay (Lowry et al. 1951), but has been modified
to save time. The DC protein assay requires only a single
15 minute incubation, and the absorbance readings arestable for at least 2 hours.
For More Information
Web: bio-rad.com/DCprotein
Download bulletins: 1069, 1731, 1770, 1909, 6837, and 6852

Ordering Information
Catalog #Description

5000111D
 C Protein Assay Kit I, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin reagent,
5 ml surfactant solution, bovine g-globulin standard; sufficient for 450 standard assays
DC Protein Assay Kit II, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin reagent,
5 ml surfactant solution, bovine serum albumin standard; sufficient for 450 standard assays

190.00Protein Assay Reagent A, 250 ml, alkaline copper tartrate solution
Protein Assay Reagent B, 1 L, dilute Folin reagent
Protein Assay Reagent S, 5 ml, surfactant solution
DC Protein Assay Reagents Package, includes 250 ml alkaline copper tartrate solution,
2 L dilute Folin reagent, 5 ml surfactant solution; sufficient for 450 standard assays

55.00
55.00
37.00
180.00Protein Standard I, bovine g-globulin, lyophilized (reconstituted volume 20 ml)
Protein Standard II, bovine serum albumin, lyophilized (reconstituted volume 20 ml)

74.00
74.00

5000112
Reagents
500011350001145000115
5000116
Accessories
5000005
5000007

See Also
Protein sample
preparation:
page 2.
Cuvettes: page 31.

Price, U.S. $175.00RC DC Protein Assay
The RC DC (reducing agent and detergent compatible)
protein assay is a colorimetric assay for protein
determination in the presence of reducing agents anddetergents. The RC DC protein assay, based on the
Lowry protocol (Lowry et al. 1951), includes the featuresof the original DC protein assay. Its compatibility witha broader range of reagents allows simplified protein
quantitation directly in complex sample solutions.
For More Information
Web: bio-rad.com/RCDCprotein
Download bulletins: 1069, 2610, 6838, and 6852

30 | Sample Quantitation

In the U.S., call toll free at 1-800-424-6723

Cuvettes
bio-rad.com/assaycuvettes

Ordering Information
Catalog #Description

5000121

 C DC Protein Assay Kit I, includes RC reagents package, DC protein assay reagents package,Rbovine g-globulin standard; sufficient for 450 standard assays
 C DC Protein Assay Kit II, includes RC reagents package, DC protein assay reagents package,Rbovine serum albumin standard; sufficient for 450 standard assays

5000122
Reagents
500011350001145000115
5000116
5000120
5000119
Accessories
5000005
5000007

Protein Assay Reagent A, 250 ml, alkaline copper tartrate solution
Protein Assay Reagent B, 1 L, dilute Folin reagent
Protein Assay Reagent S, 5 ml, surfactant solution
DC Protein Assay Reagents Package, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin
reagent, 5 ml surfactant solution; sufficient for 450 standard assays
RC DC Protein Assay Reagents Package, includes RC reagents package and DC protein assay reagents
package; sufficient for 450 standard assays
RC Reagents Package, includes RC reagent I (250 ml) and RC reagent II (250 ml); sufficient for
500 standard assays
Protein Standard I, bovine g-globulin, lyophilized (reconstituted volume 20 ml)
Protein Standard II, bovine serum albumin, lyophilized (reconstituted volume 20 ml)

Price, U.S. $295.00295.00

55.00
55.00
37.00
180.00290.00115.00

74.00
74.00

Cuvettes
Bio-Rad offers plastic cuvettes in two working volumes.

See Also

For More Information
Web: bio-rad.com/assaycuvettes

Protein assay
kits: page 28.

Standard and Semimicrovolume Cuvettes
Polystyrene cuvettes are ideal for use with protein assays(pages 28–30). They adsorb less Coomassie Bluedye than glass or quartz cuvettes. Assays can be mixed
directly in the standard 3.5 ml cuvettes, and the volumeof reagents can be reduced, yielding more assays per kit.
Semimicrovolume 1.5 ml cuvettes are ideal for precise
quantitation of small-volume samples. Both standard
and semimicrovolume cuvettes are sized to fit mostspectrophotometers. Bio-Rad cuvettes offer smooth
optical surfaces for consistent and accurate readings.
For More Information
Web: bio-rad.com/assaycuvettes
Cuvette Racks

Ordering Information
Catalog #Description

Price, U.S. $Disposable Polystyrene Cuvettes
2239950
Standard Disposable Polystyrene Cuvettes, 3.5 ml, 100
2239955
Semimicrovolume Disposable Polystyrene Cuvettes, 1.5 ml, 100

12.50
12.50

Cuvette Racks
1660485
Cuvette Racks, hold 12 cuvettes each, set of 5

73.75

In the U.S., call toll free at 1-800-424-6723

Sample Quantitation | 31

Cuvettes
bio-rad.com/assaycuvettes

Subject Index
Bio-Rad Protein Assay, 29
DC Protein Assay, 30
Quick Start Bradford Protein Assay, 28
RC DC Protein Assay, 30
Standard and Semimicrovolume Cuvettes, 31

32 | Sample Quantitation

In the U.S., call toll free at 1-800-424-6723

Cell Counting, Enumeration,
and Enrichment
Automated Cell Counting System

34

Cell Enumeration and Enrichment36Automated Cell Counting System
bio-rad.com/cellcountAutomated Cell Counting System
Video
Product
Demonstration

bio-rad.com/
TC20start

TC20 Automated Cell Counter
With its innovative auto-focus and sophisticated cellcounting algorithm, the TC20 automated cell counter
eliminates subjectivity while delivering reliable countsof mammalian cells in 30 seconds.
■

■

■

■

■

 ompatible with a broad range of cell sizes and types —Ccounts cell lines, primary cells (from tissue or blood),
and stem cells
Innovative auto-focus technology — removes thevariation associated with manual focusing and leads toprecise cell counts in 30 sec
 ell size gates — user selects a population of interest inCcomplex samples, such as primary cells, or lets the cellcounting algorithm do all the work ell viability — analyzes cells accurately using multifocal
C
plane analysis
 asy to archive and analyze — stores up to 100 counts
Ein the onboard memory for access any time, or use theoptional TC20 data analyzer software on your PC tofurther analyze exported cell images

Upon insertion of a counting slide, the TC20 provides
a total cell count with or without staining and assesses
cell viability via trypan blue exclusion. To determine if a cell
is viable, the TC20 counter analyzes each cell on images
acquired from multiple focal planes during the focusing
step. Auto-focus and multifocal plane analysis reducecounting bias associated with manual focusing.
Accuracy is comparable to results obtained with ahemocytometer. The TC20 counter can count cells witha 6–50 μm cell diameter and within a broad concentration
range of 5 x 104–1 x 107 cells/ml, which eliminates theneed to dilute cells, thus reducing the errors associatedwith sample dilutions prior to counting.

TC20 Automated Cell Counter, Counting Slides, and Trypan BlueFor complex samples composed of multiple cell
populations, such as primary cells, the position of cell size
gates can be adjusted to define the population of interest.
Results from 100 previous counts are stored in the TC20
cell counter and can be exported via the USB port.
Images of cells can be further analyzed on a computer
using the TC20 data analyzer software.
For More Information
Web: bio-rad.com/TC20
Request or download bulletins: 6282 and 6283
Learn More about the Technology
Web: bio-rad.com/tech/cellcounting

34 | Cell Counting, Enumeration, and Enrichment

In the U.S., call toll free at 1-800-424-6723

Automated Cell Counting System
bio-rad.com/cellcountOrdering Information
Catalog #Description

Price, U.S. $TC20 Automated Cell Counter
1450102
TC20 Automated Cell Counter, 120–240 V, includes instrument, power supply, USB flash drive,
30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue
1450103
TC20 Automated Cell Counter with Thermal Label Printer, 120–240 V, includes instrument,
power supply, USB flash drive, USB cable, thermal label printer, 1 roll of 500 labels, 30 dual-chambercounting slides (60 counts), 1.5 ml trypan blue
Kits and Reagents*
1450003
Counting Kit, includes 30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue
1450013Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution,sterile filtered, sufficient for 150 counts (10 µl/count), 1 x 1.5 ml
1450021
Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution,sterile filtered, sufficient for 750 counts (10 µl/count), 5 x 1.5 ml
1450022
Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution,sterile filtered, sufficient for 1,500 counts (10 µl/count), 10 x 1.5 ml
1450015
Counting Slides, 150 dual-chamber counting slides (300 counts)
1450016
Counting Slides, 300 dual-chamber counting slides (600 counts)
1450017
Counting Slides, 600 dual-chamber counting slides (1,200 counts)
1450018
Counting Slides, 900 dual-chamber counting slides (1,800 counts)
1450019
Counting Slides, 1,200 dual-chamber counting slides (2,400 counts)
1450020
Counting Slides, 2,400 dual-chamber counting slides (4,800 counts)
1450014
System Test Kit, includes verification slide, instructions
Accessories*
1450005
1450007

Thermal Label Printer, 120–240 V, includes thermal label printer, USB cable, 1 roll of 500 labels
Thermal Printer Labels, 1 roll of 500 labels, for thermal label printer4,220.00
4,750.00

53.00
9.00
44.00
82.00
270.00525.001,000.00
1,420.00
1,760.00
3,300.00
930.00700.0035.00* Compatible with TC20 and TC10 automated cell counters.

In the U.S., call toll free at 1-800-424-6723

Cell Counting, Enumeration, and Enrichment | 35

Cell Enumeration and Enrichment
bio-rad.com/celselectCell Enumeration and Enrichment
New Genesis Cell Isolation System
The Genesis system, together with Celselect Slidetechnology, enables isolation of circulating tumor cells (CTCs)
and rare cells from whole blood. The slides utilize 56,400
individual microchambers to capture cells from whole bloodbased on their size (>8 µm). The Genesis system supports
both enrichment and enumeration applications.
After capture, the enriched cells can be retrieved fordownstream analysis such as sequencing (bulk or singlecell), digital PCR, FISH, flow cytometry, or cells cultured forfurther in vitro and in vivo analysis. Alternatively, captured
cells can be stained on-slide for immunofluorescentapplications such as enumeration. The Rare Cell Analysis
software offers an integrated method to count and report
cells using images from compatible automated microscopes.
For applications that require protocol optimization or
creation of new protocols for custom reagents or antibody
stains, the Protocol Builder software tool enables changing
parameters, which include reagent dispensing, pumppressures, and both incubation temperature and timing.
Protocol optimization and customization with the Protocol
Builder tool is performed on a personal computer.
■■■

Genesis System

Size-based cell capture from liquid biopsies ecover viable cells for downstream analysis or on-slideRstaining for enumeration
 ustomizable protocols enabling optimization for differentCsample types or antibody stains

For More Information
Web: bio-rad.com/celselectOrdering Information
Catalog #Description

CEL20101

Genesis System, includes Genesis instrument, Genesis instrument accessory kit, Celselect accessory kit,and system software

36 | Cell Counting, Enumeration, and Enrichment

Price, U.S. $In the U.S., call toll free at 1-800-424-6723

Cell Enumeration and Enrichment
bio-rad.com/celselect

New Celselect Slide Technology
Celselect Slides are used with the Genesis system to gently
capture cells based on their size utilizing microfluidics and56,400 individual microchambers. Captured cells canbe stained for immunofluorescent applications such as
enumeration, or recovered for downstream analysis suchas sequencing (bulk or single-cell), digital PCR, FISH,
flow cytometry, or cells cultured for further in vitro and invivo analysis. After staining, the cells are counted on an
automated microscope. The high capture efficiency andcustomizability make Celselect Slide technology the ideal
approach to capture and analyze cells.
For the enumeration application, there are two approaches
used to stain cells on-slide. One utilizes indirect staining
where primary antibodies attach to cell-specific surface
antigens, which are subsequently bound by secondary
antibodies that fluoresce at amplified levels. The second
approach, direct staining, utilizes a conjugated antibody
that binds and fluoresces specifically at the antigen.
Essentially, direct staining achieves higher specificity at
the cost of lower fluorescent signal intensity and indirect
staining achieves higher signal intensity at the cost of lower
specificity. Pre-defined protocols and kits are available
for each staining method. Both methods permeabilize thecell membrane, facilitating the use of additional cytosolic
markers, as well as surface markers.
The Rare Cell Analysis software, installed on a personal
computer, analyzes images of cells captured andfluorescently stained on Celselect Slides and generates
a report. The software accepts images in standard
formats such as .tiff and .png from many of the available
automated microscopes and is compatible with both Mac
and PC.

Celselect Slide Enrichment
Capture and recover of >80% of CTCs fromliquid biopsies■■ ith blood sample, 99.9% elimination rate of RBCs,
W
WBCs, and other components■

High cellular viability■

Enriched cells available for downstream analyses

Celselect Slide Enumeration
Highly efficient capture of CTCs and rare cells fromliquid biopsies■■

On-slide immunostaining■

Imaging on automated microscope■

Rare Cell Analysis software for result reporting

For More Information
Web: bio-rad.com/celselectOrdering Information
Catalog #Description

CEL80110

Celselect Enrichment Kit, includes reagents and consumables for enrichment of cells from 4 samples

CEL80111

Celselect Enumeration Indirect Stain Kit, includes reagents and consumables for the enrichment andstaining of 4 samples
Celselect Enumeration Direct Stain Kit, includes reagents and consumables for the enrichment andstaining of 4 samples

CEL80112

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $4,220.00

Cell Counting, Enumeration, and Enrichment | 37

Cell Counting, Enumeration, and Enrichment
Subject Index

Subject Index
TC20 Automated Cell Counter, 34
Genesis Cell Isolation System, 36
Cellselect Slide Technology, 37

38 | Cell Counting, Enumeration, and Enrichment

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry
Cell Sorting
Consumables

40
40

Cell Analysis
Instruments
Instrument Upgrades
Reagents

45
45
48
51

Cell Biology Reagents
Antibody Labeling Kits
Cell Proliferation Assays
Cell Proliferation Detection Antibodies
Cell Viability Assays
Flow Cytometry Calibration and Validation Reagents
Flow Cytometry General Reagents
Nuclear Staining Dyes

52
52
56
60
61
64
66
68

Regulated Cell Death Reagents
Apoptosis Reagents
Autophagy Reagents
Pyroptosis Reagents

70
70
75
77

Cell Sorting
Consumables

bio-rad.com/cellsorting

Cell Sorting
Cell sorting provides a method, typically using fluorescence, to sort or isolate a homogenous population of cells from aheterogeneous mixture of cells based on intracellular and/or extracellular properties. Expressed fluorescent proteins, suchas green fluorescent protein (GFP), or fluorophores conjugated to antibodies bind to markers of interest, which allows for astarting population to be sorted to greater than 99% purity. While cell sorting is commonly used in fields such as immunology,
cancer biology, and stem cell biology, it is quickly becoming a useful tool in broader research studies from microbiology toneuroscience. Cell sorting can also be used as an upstream tool for proteomic or genomic experiments to achieve more
specific results by reducing background noise or false positives.

Consumables
S3e Cell Sorter Consumables

Ordering Information
Catalog #Consumables
1441001
1441002
1441003
1441005
1441006
1441007
1441008
1441009
144101014410111441012144101314510651451086

Description

Price, U.S. $S3e Sheath Cap Assembly, whiteS3e DI Water Cap Assembly, blue
S3e Waste Cap Assembly, red
S3e Filter Block A, empty with 2 dichroic filter holders
S3e Filter Block B, empty with 2 dichroic filter holders
S3e Individual Filter Holder, black, empty
Neutral Density Filter, optical density 1.0
S3e Nozzle Tip, 100 µm
S3e Nozzle O-Ring with Alignment Disk, pkg of 2
S3e Collection Adaptor Set
Hex Driver, 2 mm
S3e Individual Dichroic Filter Holder, empty
S3e Cell Sorter Accessory Kit, includes 100 μm nozzle tip, 2 nozzle O-rings, 2 nozzle alignment
disks, 1 ml syringe, 2 neutral density filters (1.0), 2 mm hex driver, spanner wrench
ProLine Universal Calibration Beads, includes 3 x 5 ml bottles of ready-to-use beads for alignment
verification and drop delay determination on the S3e cell sorter

250.00250.00250.00850.00850.00100.0080.00
600.0058.00
290.009.00
100.001,000.00
490.0012012932

ProFlow Sort Grade 8x Sheath Fluid, includes 5 x 4 L ready-to-use, sterile, preservative-freephosphate buffered saline (PBS) solutions in gamma-irradiated S3e fluidic containers

490.001451083

 roFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiatedP
S3e fluidic containers

390.001451084

S3e Fluidic Container, 3 x 4 L gamma-irradiated sterile containers for use on the S3 or
S3e cell sorter
ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 8 different
fluorescence intensities for excitation at wavelengths between 365–650 nm

78.00

1451085

40 | Flow Cytometry

460.00In the U.S., call toll free at 1-800-424-6723

Cell Sorting
bio-rad.com/cellsorting

Consumables

S3e Cell Sorter IQ/OQ Services
Installation qualification (IQ) and operational qualification (OQ) services for the S3e cell sorter ensure that your system
functions properly and aids in regulatory compliance. Both services are completed by a certified service engineer.
IQ services include a careful inspection and documentation of instrument installation. The engineer ensures that the
instrument is properly installed, inspects vital system components, and documents details about the computer softwareand vital system components.
OQ services verify and document that the instrument is performing according to the manufacturer’s specifications. The
thorough evaluation includes documentation of essential system information, evaluation of all key system components,
complete instrument verification, service system testing, manual and bead-based testing, and signed documentation of
the completion of the IQ/OQ services.
■■■ aximize instrument uptime — planned instrument qualification services ensure your instrument is performingMaccording to specifications
Increase your confidence — frequent instrument validation helps minimize loss of precious samples and valuable timeassociated with a malfunctioning instrument
Remain compliant — official documentation can aid in meeting regulatory requirements

For More Information

Web: bio-rad.com/flowiqoq

Ordering Information
Catalog #Description

12005873

S3e Cell Sorter IQ/OQ Kit, includes IQ and OQ protocols, test reagents, and accessories

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $390.00Flow Cytometry | 41

Cell Sorting
Consumables

bio-rad.com/cellsorting

ProLine Universal Calibration Beads
ProLine universal calibration beads are designed to verify
the alignment performance and determine the drop delay
on any S3 or S3e cell sorter. Each bottle contains a single
population of ready-to-use fluorescent beads for a total of
30 quality control (QC) tests.
Product features include:
■

■

■

■

Ready-to-use fluorescent beads do not require dilution
 lignment and drop delay can be performed withA
1 reagent in less than 7 min
Pass/fail performance result with ProSort software rending QC information in ProSort software for trackingTthe performance of your S3 or S3e cell sorter

For More Information
Web: bio-rad.com/prolineuniversal
Download bulletin: #10041517
Request or download bulletin: 6614

Ordering Information

See Also
ProFlow sort grade
8x sheath fluid:
page 43.
ProFlow sortgrade water:
page 44.
S3e fluidic container:
page 44.

Catalog #Description

1451086

ProLine Universal Calibration Beads, 15 ml (3 x 5 ml)

Price, U.S. $490.00

ProLine Rainbow Beads
ProLine rainbow beads are designed for instrument
performance monitoring on a flow cytometer system.
Each bottle contains 3.0–3.4 µm beads dyed with eight
different fluorescent intensities for excitation by lasers
between 365 and 650 nm.
■■■

 asily check the sensitivity and linearity of your flowEcytometer with one set of beads
 rack fluorescent fluctuations with validated bead lotsTto ensure accurate baselines
 se with either the S3e cell sorter or the ZE5
Ucell analyzer

For More Information
Web: bio-rad.com/prolinerainbowbeads
Download bulletin: #10033040

Ordering Information
Catalog #Description

1451085

ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with
8 different fluorescent intensities for excitation at wavelengths 365–650 nm

42 | Flow Cytometry

Price, U.S. $460.00In the U.S., call toll free at 1-800-424-6723

Cell Sorting
bio-rad.com/cellsorting

Consumables

See Also

ProFlow Sort Grade 8x Sheath Fluid

ProLine universal
calibration beads:
page 42.

ProFlow sort grade 8x sheath fluid is a ready-to-use,sterile, endotoxin- and preservative-free phosphatebuffered saline solution. Each lot is tested for cytotoxicity
and particulates and is bottled in gamma-irradiatedcontainers to ensure sterility. Up to 5 weeks of run time
is available per case when it is used on the S3 or S3ecell sorter. The S3 or S3e cell sorter employs its internal
fluidics system to automatically dilute ProFlow sort grade
8x sheath fluid and creates 32 L of 1x sheath solution fromeach container, generating 160 L per case.

ProLine rainbow
beads: page 42.
ProFlow sortgrade water:
page 44.
S3e fluidic container:
page 44.

Product features include:■■■ o manual dilution required — the S3 and S3e cellNsorters’ unique internal fluidics chamber automaticallygenerates a 1x solution, ensuring accurate dilution andminimizing potential contamination
 asy container swapping — prepackaged, sterile S3eE
fluidic container bypasses the need to fill a new container
during a sort, minimizing the risk of contamination
 inimum storage requirements — one 20 L caseMcreates 160 L of 1x sheath solution, freeing your storage
space for other purposes

For More Information
Web: bio-rad.com/sheathfluid
Download bulletin: #10030825
Request or download bulletin: 6614

Ordering Information
Catalog #Description

12012932

ProFlow Sort Grade 8x Sheath Fluid, 20 L (5 x 4 L)

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $490.00Flow Cytometry | 43

Cell Sorting
Consumables

See Also
ProLine universal
calibration beads:
page 42.
ProLine rainbow
beads: page 42.
ProFlow sort grade
8x sheath fluid:
page 43.

bio-rad.com/cellsorting

ProFlow Sort Grade Water
This sterile, endotoxin-free solution is ideal for flow
cytometry instrumentation. ProFlow sort grade water isdesigned to provide the best sort results when combinedwith preservative-free ProFlow sort grade 8x sheath fluidon any S3 or S3e cell sorter. It may also be used on theZE5 cell analyzer. Each lot is prepared by reverse osmosis,
passed through fine carbon, deionized through two resin
beds, serially filtered twice through 0.1 μm positively
charged membranes, and poured into gamma-irradiatedS3e fluidic containers.
For More Information
Web: bio-rad.com/proflowsortgradewater
Download bulletin: #10032248

Ordering Information
Catalog #Description

1451083

 roFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiatedP
S3e fluidic containers

Price, U.S. $390.00S3e Fluidic Container
S3e fluidic containers are specifically designed for useon the S3 or S3e cell sorter. Easily store your solutionsin the S3e fluidic containers for quick container swaps
during a run. Each 4 L container is gamma irradiatedto ensure sterility.
For More Information
Web: bio-rad.com/s3emore
Request or download bulletin: 6614

Ordering Information
Catalog #Description

1451084

S3e Fluidic Container, 3 x 4 L gamma-irradiated sterile containers for use on the
S3 or S3e cell sorter

44 | Flow Cytometry

Price, U.S. $78.00

In the U.S., call toll free at 1-800-424-6723

Cell Analysis
bio-rad.com/ZE5

Instruments

Cell Analysis
Cell analysis via flow cytometry examines the physical and chemical characteristics of particles in a fluid as it passes
through at least one laser. Cell components are usually fluorescently labeled and then excited by the laser to emit light atvarying wavelengths. Flow cytometry allows fast analysis at the single-cell level. Typical parameters include cell volume(forward scatter), morphological complexity (side scatter), pigments, DNA content, protein expression, cell surface andintracellular antigens, cytokines, apoptosis, cell viability, cell monitoring, and fluorescent proteins. Life science disciplinesthat utilize flow cytometry include immunology, cell biology, cancer research, plant biology, microbiology, pathology,
marine biology, and medicine.

See Also
Cell biology
reagents:
page 52.

Instruments
ZE5 Cell Analyzer
The ZE5 cell analyzer was created by gathering input
from some of the leading flow cytometer users across theworld and was designed to address the gaps in currently
available systems.
It is an innovative flow cytometer with flexible configurations
designed to meet a broad range of experimental
complexities and throughput needs. With up to 30
detectors (27 colors), the ZE5 cell analyzer is a truly
expandable flow cytometer. The fully integrated sample
loader, with the ability to handle tubes or plates withoutchanging instrument hardware, enables true walk-away
functionality. Features such as the stat tube station, the fluid
and reagent hot swap capability, and the automated startup
and QC allow for continuous operation and maximize
instrument running time for any busy core lab. With its API,the ZE5 cell analyzer can be integrated into any workcell for
automated analysis. Its innovative sample delivery system
ensures quick processing, reduced carryover, and minimal
sample loss by returning unused precious cells to thesample plate or tube for later use.
Features include:■

Flexible loading options■

Best-in-class and scalable optics■

Integrated and easy-to-use fluidics■

Innovative electronics providing highest quality data■

High-throughput production■

Automation/robotics capability

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 45

Cell Analysis
Instruments

bio-rad.com/ZE5Specifications
System

OpticsElectronics

Fluidics

Installation

SoftwareRegulatory

Fluorescence sensitivity<100 MESF for FITC, PE, APC

Scatter sensitivity<0.3 µm FSC resolution with small particle detection module

LoaderIntegrated sample loader with agitation and temperature control. Up to 384-well plates;
40-tube rack for 5 ml 12 x 75 mm tube loading. Stat tube position for single tube loading

Excitation

Up to five spatially separated lasers per instrument. Standard options include:
355 nm, 50 mW

405 nm, 100 mW

488 nm, 100 mW

640 nm, 100 mW

561 nm, 50 mW

Detection

Up to 30 detectors including FSC and SSC; optional second FSC detector

CuvetteFused silica with 145 x 265 µm channel

Speed>100,000 events per second with all parameters enabled

Data processing

Simultaneous measured peak, area, and width for every channel. 24-bit data for peak andarea. 17-bit data for width with high-resolution linear interpolation at the half height.

Sample flow rates

0.0025–2.5 µl/sec

Bulk fluids

4 x 4 L bulk fluid tanks on board for sheath and waste. Onboard additive concentrateand cleaner.

Power

95–240 VAC, 50–60 Hz, <500 W

Dimensions (W x D x H)

29 x 27 x 26 in.; 74 x 69 x 66 cm

Weight<240 lb; <110 kg

Temperatureand humidity

18–25°C; 20–60% RH

Air and vacuum supply

Included, onboard

Operating system

Windows 7 Pro

FCS format

FCS 3.1

QC

Automated quality control with onboard calibration beads

Workstation

Intel Core i7-7700 Quad Core; 3.3 GHz

CE, Class I (1) laser productResearch use only

For More Information
Web: bio-rad.com/ZE5
Request or download bulletin: 6814

Ordering Information
Catalog #Description

Price, U.S. $ZE5 Cell Analyzer
12004276
12014141
12004277
12014139
12014140
12004278
12014138
12004279
12014135
12014136

ZE5 Cell Analyzer, 3 laser 17 color (405/488/640 nm)
ZE5 Cell Analyzer, 3 laser 17 color (405/488/640 nm) with SPD
ZE5 Cell Analyzer, 3 laser 17 color (488/561/640 nm)
ZE5 Cell Analyzer, 3 laser 17 color (405/488/561 nm)
ZE5 Cell Analyzer, 3 laser 20 colors (405/488/561 nm) with SPD
ZE5 Cell Analyzer, 4 laser 24 color (405/488/561/640 nm)
ZE5 Cell Analyzer, 4 laser 24 color (405/488/561/640 nm) with SPD
ZE5 Cell Analyzer, 5 laser 27 color (355/405/488/561/640 nm)
ZE5 Cell Analyzer, 5 laser 27 color (355/405/488/561/640 nm) with 7 off UV option A
ZE5 Cell Analyzer, 5 laser 27 color (355/405/488/561/640 nm) with 7 off UV option B

78.00
78.00

78.00
78.00

continues

46 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Cell Analysis
Instruments

bio-rad.com/ZE5

Ordering Information
Catalog #Description

Price, U.S. $Accessories
12004403
1451085
12004271
12004404
12004272
12004395
12004444
1451083
12004397
12004396
12004418
12004402
12004274
12004445
12004446

ZE-Series QC Beads, 5 ml, pack of 3
ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 8
different fluorescent intensities for excitation at wavelengths between 365–650 nm
ZE5 Additive, 4 x 300 ml
ZE5 Additive Bottle with Tubing, white, includes 450 ml bottle, cap/tubing assembly with filterand quick connector
Cytometer Cleaner, 1 L
ZE5 Cleaner Bottle with Tubing, blue, includes 450 ml bottle, cap/tubing assembly with filterand quick connector
ZE5 Cleaner/Additive Bottle, includes 2 x 450 ml bottles
ProFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiatedfluidic containers
ZE5 DI Water Container with Tubing, includes 4 L container, cap/tubing assembly with filtersand quick connectors
ZE5 Waste Container with Tubing, includes 4 L container, cap/tubing assembly with filters andquick connectors
ZE5 DI Water Container, 4 L, pkg of 2
ZE5 Waste Container, 4 L, pkg of 2
5 ml Tubes, 12 x 75 mm with cap, sterile polypropylene, pack of 25
ZE5 Tube Rack, 5 ml size, 40 spots
ZE5 Tube Rack, 1.5 ml size, 24 spots

78.00
78.00

78.00
78.00

ZE5 Cell Analyzer IQ/OQ Services
Installation qualification (IQ) and operational qualification(OQ) services for the ZE5 cell analyzer ensure thatyour system functions properly and aids in regulatorycompliance. Both services are completed by a certified
service engineer.
IQ services include a careful inspection and documentationof instrument installation. The engineer ensures that the
instrument is properly installed, inspects vital systemcomponents, and documents details about the computer
software and vital system components.

■

■

■

 aximize instrument uptime — planned instrument
M
qualification services ensure your instrument isperforming according to specifications
Increase your confidence — frequent instrument
validation helps minimize loss of precious samples andvaluable time associated with a malfunctioning instrument
 emain compliant — official documentation can aid inRmeeting regulatory requirements

For More Information
Web: bio-rad.com/flowiqoq

OQ services verify and document that the instrument isperforming according to the manufacturer’s specifications.
The thorough evaluation includes documentation of
essential system information, evaluation of all key systemcomponents, complete instrument verification, service
system testing, manual and bead-based testing, and signed
documentation of the completion of the IQ/OQ services.

Ordering Information
Catalog #Description

12005874

ZE5 Cell Analyzer IQ/OQ Kit, includes IQ and OQ protocols, test reagents, and accessories

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $78.00

Flow Cytometry | 47

Cell Analysis
Instrument Upgrades

bio-rad.com/ZE5

Instrument Upgrades
7 Detectors off 355 nm Laser Upgrade Kit
This upgrade kit expands experimental options byincreasing the number of detectors off the 355 nm laserfrom five to seven.

Compatible systems: ZE5 systems with 5 lasers

Option A

Seven Detectors

Fluorophores

Option B

Seven Detectors

Fluorophores

355 Laser
			
			
			
			
			
			
			
			
			
			
			
			

387/11439 DLP (changeable)
509/24560 DLP
577/15600 DLP (changeable)
615/24640 DLP
670/30690 DLP
747/33776 DLP (changeable)
780/LP

BUV395355 Laser
			
			
			
			
			
			
			
			
			
			
			
			

387/11439 DLP (changeable)
460/22493 DLP
509/24530 DLP (changeable)
577/15640 DLP
670/30690 DLP
747/33776 DLP (changeable)
780/LP

BUV395

BUV496, DAPIBUV563
BUV615
BUV661
BUV737
BUV805For More Information
Web: bio-rad.com/ZE5

DAPI
BUV496
BUV563
BUV661
BUV737
BUV805

Ordering Information
Catalog #Description

Price, U.S. $12009028

7 Detectors off 355 nm Laser Upgrade Kit, Option A

78.00

12009029

7 Detectors off 355 nm Laser Upgrade Kit, Option B

78.00

355 nm Laser Upgrade Kit for ZE5 Cell Analyzer
The UV (355 nm) laser addition is suitable for any ZE5
system without UV laser. It adds the 355 nm laser and bankwith five detectors. Use this laser upgrade kit to expand
your experimental options in research applications.

Compatible systems: any ZE5 system without a UV laser
For More Information
Web: bio-rad.com/ZE5

Ordering Information
Catalog #Description

12009185

355 nm Laser Upgrade Kit for ZE5 Cell Analyzer

48 | Flow Cytometry

Price, U.S. $78.00

In the U.S., call toll free at 1-800-424-6723

Cell Analysis
Instrument Upgrades

bio-rad.com/ZE5

Everest Software Developer Kit (ZE5 Automation)
The Everest application program interface (API) andsoftware development kit (SDK) allow the ZE5 cell analyzer
to interface with existing automation systems.
■■

 he ZE5 API is an industry-standard HTTP-based
T
RESTful web service that is platform- and languageindependent■It includes a fully functional API client test application,
which demonstrates how automation scheduling clientscan be implemented using status polling with a minimal
amount of coding effort. Source code is included

For More Information
Web: bio-rad.com/ZE5

 he ZE5 API solution provides all basic ZE5 functionality
Tand many advanced features designed for automatedenvironments

Ordering Information
Catalog #Description

12009761

Everest Software Developer Kit (ZE5 Automation)

Price, U.S. $78.00

Small Particle Detector (SPD) Upgrade Kit (on 405 nm Laser)This upgrade kit makes an SPD (secondary FSC detector
off the 405 nm laser) available for 3 and 4 laser ZE5
systems with a 405 nm laser. Note: The SPD comesstandard on the 5 laser ZE5 cell analyzer.

For More Information
Web: bio-rad.com/ZE5

Ordering Information
Catalog #Description

12009372

Small Particle Detector (SPD) Upgrade Kit (on 405 nm Laser)

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $78.00

Flow Cytometry | 49

Cell Analysis
Instrument Upgrades

bio-rad.com/ZE5

Large Capacity Fluidics Options
External fluidics connections reduce day-to-day fluidics
maintenance and enable uninterrupted overnight runs inhigh-throughput and automation environments.
The following options are available:■

External house DI upgrade kit■

External DI water carboy and fluidics cart upgrade kit■

External waste carboy and fluidics cart

The external house DI water upgrade kit is suitable forlaboratories with access to pressurized house DI water. It
enables a connection between the ZE5 cell analyzer andthe laboratory’s source of pressurized house DI water.

Laboratories without pressurized house DI water canuse the the external DI water carboy and fluidics cart
upgrade kit to increase the time the ZE5 cell analyzer can
run unattended. It enables approximately 22 hours of
uninterrupted run time. Liquids are hot swappable, andfluidics level warnings from Everest software inform usersthat fluidics need attention starting at 2 hours of remaining
run time.
The external waste carboy and fluidics cart upgrade kit
enables approximately 22 hours of unattended waste
collection. Liquids are hot swappable.
For More Information
Web: bio-rad.com/ZE5

Ordering Information
Catalog #Description

12009651

External House DI Water Upgrade Kit

12009707

ZE5 External DI Water Carboy and Fluidics Cart Upgrade Kit, includes external DI water
fluidics cart, power supply, 20 L DI water carboy, and DI water carboy cap vented with integrated
replaceable filter and leak-free quick disconnect

12009734

ZE5 External Waste Carboy and Fluidics Cart Upgrade Kit, includes external waste fluidics
cart with power supply, waste carboy, and cap for ZE5 waste carboy with overflow sensor, leakfree quick disconnect, and filtered vent50 | Flow Cytometry

Price, U.S. $78.00

In the U.S., call toll free at 1-800-424-6723

Cell Analysis
Reagents

bio-rad.com/ZE5

Reagents
Kits and Reagents for Cell Analysis
Bio-Rad offers a variety of easy-to-use kits and reagents suitable for use with the ZE5 cell analyzer.

Ordering Information

Catalog #Description

Antibody Labeling Kits
1351011
ReadiLink 405/454
1351002
ReadiLink 492/516
1351003
ReadiLink 555/570
1351004
ReadiLink 594/610
1351005
ReadiLink 633/655
1351006
ReadiLink 647/674
1351007
ReadiLink 680/701
1351008
ReadiLink 700/713
Cell Proliferation
1351202
CytoTrack Blue 403/454
1351203
CytoTrack Green 511/525
1351205
CytoTrack Red 628/643
1351201
CFDA-SE Cell Proliferation
Assay

Maximum
Excitation,nm

Maximum
Emission,nm

OptimalExcitation
Laser, nm

ZE5 Cell Analyzer
Configuration

403
492
555
594
633
647
680
700

454
516
570
610
655
674
701
713

405
488
561
561
640
640
640
640

405 laser 460/22
488 laser 525/35
561 laser 577/15
561 laser 615/24
640 laser 670/30
640 laser 670/30
640 laser 720/60
640 laser 720/60

403
511
628
492

454
525
643
517

405
488
633 or 640
488

405 laser 460/22
488 laser 525/35
640 laser 670/30
488 laser 525/30

38.00
38.00

355 laser 447/60
405 laser 460/22
405 laser 525/50
488 laser 525/35
561 laser 577/15
561 laser 615/24
640 laser 670/30
355 laser 670/30
488 laser 593/52
561 laser 615/24
488 laser 692/80
561 laser 640/20

38.00
38.00
38.00

355 laser 447/60
or 405 laser 460/22
355 laser 447/60 or 670/30

38.00

Cell Viability
1351111135111213511131351115135111613511171351118
1351101

VivaFix 353/442
VivaFix 410/450
VivaFix 408/512
VivaFix 498/521
VivaFix 547/573
VivaFix 583/603
VivaFix 649/660
Propidium Iodide

353
410
408
498
547
583
649
538

442
450
512
521
573
603
660
617

355
405
405
488
561
561
640
355, 488, or 561

1351102

7-AAD

548

648

488 or 561

Nuclear Dyes
1351303

DAPI

359

461

355 or 405

1351304

Hoechst 33342

350

461

355

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $405.00405.00405.00

38.00

38.00

Flow Cytometry | 51

Cell Biology Reagents
Antibody Labeling Kits

bio-rad.com/readilink1

Cell Biology Reagents
In addition to cell biology reagents on bio-rad.com, Bio-Rad offers a dedicated antibody website — bio-rad-antibodies.com— specifically designed to meet the unique needs of researchers who use antibodies. This website showcases the range of ourthousands of antibodies and related reagents suitable for flow cytometry, immunohistochemistry, immunofluorescence, western
blotting, immunoprecipitation, and ELISA. The antibody range also includes the highly validated PrecisionAb antibodies forwestern blotting and covers key research areas such as immunology, cancer, veterinary immunology research, and cell biology.

Antibody Labeling Kits
See Also

Cell counting:page 34.

ReadiLink Antibody Labeling Kits
The following products are available on bio-rad.com.

Antibod

ReadiLink antibody labeling kits offer easy fluorescence
conjugation in microscale volumes. Each kit provides all theessential components to perform two 50 μg conjugation
reactions with primary antibodies. Available in a variety
of excitation and emission wavelength combinations, theReadiLink kits can be used to rapidly conjugate any antibody
of interest for use in multicolor flow cytometry experiments.
■

Efficient two-step microgram antibody labeling■Stable carboxamide bond with fluorescent dye■

Quench buffer eliminates background fluorescence

LabeledQuenchMix andincubate

Quenchandincubate

Antibody

Quickly label your antibody ofinterest in two easy steps with
ReadiLink antibody labeling kits.

For More Information
Web: bio-rad.com/readilink1
Download bulletin: #10033055
Request or download bulletin: 6523

Normalized intensity

Dye

Dye
Labeled antibody
Quenched dye

Mix andincubate

100
80
60
40
20
0

Normalized intensity

Cell sorting
consumables:
page 40.

Quenchandincubate

100
80
60
40
20
0

250

300

350

400

450

500

550

600

650

700

750

800

850

Wavelength, nm

Excitation wavelength spectrum. ReadiLink dyes 405/454 (–);
492/516 (–); 555/570 (–); 594/610 (–); 647/674 (–); 680/701 (–);
750/780 (–); 790/811 (–).

52 | Flow Cytometry

900

250

300

350

400

450

500

550

600

650

700

750

800

850

900

Wavelength, nm

Emission wavelength spectrum. ReadiLink dyes 405/454 (–);
492/516 (–); 555/570 (–); 594/610 (–); 647/674 (–); 680/701 (–);
750/780 (–); 790/811 (–).

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagents
Antibody Labeling Kits

bio-rad.com/readilink1

Ordering Information
Maximum
Excitation,nm

Maximum
Emission,nm

OptimalExcitation
Laser, nm

ZOE
ZE5
S3e
Cell
Cell
Cell
Sorter Analyzer Imager

Catalog #Description

Fluorophore
Equivalent

1351011

ReadiLink 405/454

Pacific Blue

403

454

405

•

•

1351002

ReadiLink 492/516

FITC
Alexa Fluor 488
DyLight 488

492

516

488

•

•

•

1351003

ReadiLink 555/570

Cy3Alexa Fluor 555
DyLight 550

555

570

561

•

•

•

1351004

ReadiLink 594/610

Texas Red
Alexa Fluor 594
DyLight 594

594

610

561

•

•

•

1351005

ReadiLink 633/655

Cy5Alexa Fluor 633
DyLight 633

633

655

640

•

•

1351006

ReadiLink 647/674

Cy5Alexa Fluor 647
DyLight 650

647

674

640

•

•

1351008

ReadiLink 700/713

Alexa Fluor 700

700

713

640

•

•

Price, U.S. $405.00405.00405.00Each ReadiLink kit includes 2 vials of labeling dye, reaction buffer, and dye quench for labeling 2 x 50 µg of antibodies.

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 53

Cell Biology Reagents
Antibody Labeling Kits

bio-rad.com/lynxkits

LYNX Conjugation Kits

5
10

The following products are available on
bio-rad-antibodies.com.

4
10

15 min* or 3 hr

■

■

■

■

0
10

44
10
10

44
10
10

CD8 DyLight650

55
10
10

33
10
10

22
10
10

1
10

2
10

3
10

4
10

5
10

101044

101055

33
10
10

22
10
10

11
10
10

0
10 0

10

CD8 DyLight 650

CD8 A647

55
10
10

1
10 1
10

Instantly ready to use — no additional columns or
dialysis are required after the conjugation process isfinished; the stable, high-quality labeled antibodies areready to use

Easy scale-up — same procedure is used for labelingsmall and large quantities of antibodies; users can initiallyconjugate micrograms of antibody for trial, then scale up
to milligram quantities

Add LYNX quencher andincubate >30 min

CD4 PESimple — can be used in the presence of up to
0.1% sodium azide and 0.5% bovine serum albumin 00% recovery — no postconjugation cleanup required,1
which enables 100% yields of labeled antibodies. LYNX
kits save time and eliminate batch-to-batch variation andloss of antibodies compared to traditional labeling methods

2
10

1

CD8 A647

 ast — 1-step labeling procedure; requires 30 sec
Fhands-on time, with an incubation time of either 15 minwith the LYNX Rapid Plus kits or 3 hr with the LYNX
Rapid kits to finish the conjugation

Ready to use

310

Add the antibody10modifier
mixture to LYNX mix and0
incubate for 15 min*10 or 3 hr

Add LYNX modifier topurified antibodies

0

0 0
1010

11
1010

101022101033
CD4 PE

10104 4

101055

CD4 PE

CD8 DyLight650■

CD8 A647

LYNX Rapid Conjugation Kit and LYNX Rapid Plus
conjugation kits are available in a wide range of options,
including common fluorophores, enzymes, and biotin/
streptavidin conjugations. These kits are suitable for use
in flow cytometry, enzyme-linked immunosorbent assay(ELISA), western blotting, immunohistochemistry, andimmunofluorescence applications. Identical protocols forsmall-scale and large-scale conjugations allow conjugationof up to milligram quantities of antibody. Conjugate yourantibodies in three easy steps with only 30 seconds
hands-on time and no purification required.

10 0
10
00
1010

10101 1101022101033
CD4 PE
CD4 PE

Comparison of LYNX kits with directly conjugated antibodies.
5
10
Purified
CD4 and CD8 T cells were labeled with LYNX Rapid RPE
antibody conjugation kit (catalog #LNK021RPE) and LYNX Rapid Plus
4
10
DyLight
650 antibody conjugation kit (#LNK241D650), respectively, andused to stain human peripheral blood. Alternatively, directly labeled
3
10antibodies
were used. The staining shown is lymphocytes gated on
the CD3 positive population. CD4 and CD8 positive T cells can be2
10
identified
in both plots. Data acquired on the ZE5 cell analyzer.
PE, phycoerythrin.
1
10* LYNX
Rapid Plus kits only.
0
10

0
10

1
10

2
10

3
10

4
10

5
10

CD4 PEFor More Information
Web: bio-rad.com/lynxkits

54 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagentsbio-rad.com/lynxkits

Antibody Labeling Kits

Ordering Information
Catalog #Description

Price, U.S. $LYNX Rapid Conjugation Kits
LNK061FLYNX Rapid Fluorescein Antibody Conjugation Kit, 3 conjugations for 200 µg antibody

78.00

LNK062F
LNK063F
LNK021RPE
LNK022RPE
LNK023RPE
LNK024RPE
LNK171PETR
LNK172PETR
LNK173PETR
LNK174PETR
LNK031APC
LNK032APC
LNK033APC
LNK034APC
LNK082C
LNK084C
LNK071PERCPLNK072PERCPLNK073PERCPLNK141PERCPCY5.5
LNK142PERCPCY5.5
LNK143PERCPCY5.5
LNK144PERCPCY5.5
LNK094PECY5.5
LNK131APCCY7
LNK132APCCY7
LNK133APCCY7
LNK134APCCY7
LNK111PECY7
LNK112PECY7
LNK113PECY7
LNK114PECY7LYNX Rapid Fluorescein Antibody Conjugation Kit, 1 conjugation for 2 mg antibody
LYNX Rapid Fluorescein Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LYNX Rapid RPE Antibody Conjugation Kit, 1 conjugation for 60 µg antibody
LYNX Rapid RPE Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LYNX Rapid RPE Antibody Conjugation Kit, 1 conjugation for 600 µg antibody
LYNX Rapid RPE Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid APC Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LYNX Rapid APC Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LYNX Rapid APC Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LYNX Rapid APC Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid RPE-Cy5 Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LYNX Rapid RPE-Cy5 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid PerCP Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LYNX Rapid PerCP Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LYNX Rapid PerCP Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid RPE-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 1 conjugation for 60 µg antibody
LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 1 conjugation for 600 µg antibody
LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody

78.00

78.00
78.00

LYNX Rapid Plus Conjugation Kits
LNK221D488
LYNX Rapid Plus DyLight 488 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK222D488
LYNX Rapid Plus DyLight 488 Antibody Conjugation Kit, 1 conjugation for 2 mg antibody
LNK223D488
LYNX Rapid Plus DyLight 488 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK201CY3LYNX Rapid Plus Cy3 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK203CY3LYNX Rapid Plus Cy3 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK251RHOLYNX Rapid Plus Rhodamine Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK253RHOLYNX Rapid Plus Rhodamine Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK231D550
LYNX Rapid Plus DyLight 550 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK233D550
LYNX Rapid Plus DyLight 550 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK211CY5LYNX Rapid Plus Cy5 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK212CY5LYNX Rapid Plus Cy5 Antibody Conjugation Kit, 1 conjugation for 2 mg antibody
LNK241D650
LYNX Rapid Plus DyLight 650 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK243D650
LYNX Rapid Plus DyLight 650 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 55

Cell Biology Reagents
Cell Proliferation Assays

bio-rad.com/cellproliferationassay

Cell Proliferation Assays
An increase or decrease in cell growth and division can be a key result in cytotoxicity and apoptosis assays and can be used
to determine the effect of experimental treatments on cellular replication. We offer a broad range of antibodies, kits, and dyes
to monitor proliferation. Proliferation can be measured through dye incorporation into live cells, by quantifying key proliferation
markers, or by assessing incorporation of the thymidine analog BrdU into DNA.

Cell Proliferation Assay Reagents■■The following products are available on
bio-rad-antibodies.com.
alamarBlue is a cell proliferation assay that provides a rapid,
sensitive, and economical way to quantitatively measurecell proliferation and cytotoxicity in various human andanimal cell lines. It can also be used to study mycobacteria,bacteria, and fungi. It is a preferred alternative reagent tosimilar cell proliferation assays, including MTT, XTT, 3H
thymidine, and neutral red.

■

■

■

■

■

For More Information
Web: bio-rad.com/cellproliferationassay

 imple — water soluble, just add and measure theSformula in cell cultures lexible — colorimetric and fluorometric detection forFboth suspension and adherent cell lines
 ontoxic — alamarBlue does not kill cells and isNsafe to the user and the environment
Reliable — highly referenced for cytotoxicity andviability assays
High sensitivity — detects as few as 50 cells
 table — proprietary buffering agents ensure alamarBlue
S
is suitable for time-course studies
 conomical — lack of cell lysis allows cells to beE
cultured for subsequent assays

Ordering Information
Catalog #Description

BUF012A
BUF012B

alamarBlue Reagent, 25 ml
alamarBlue Reagent, 100 ml

56 | Flow Cytometry

Price, U.S. $305.00305.00In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagents
Cell Proliferation Assays

bio-rad.com/cytotrack

CytoTrack Cell Proliferation Assay
The following products are available on bio-rad.com.

CytoTrack Blue 403/454 (DPBS)

CytoTrack Green 511/525 (DPBS)
100

Days 5 and 6
not collected

80

Normalized to mode

Normalized to mode

CytoTrack cell proliferation assays efficiently stain live
cells for excellent resolution of up to 10 generations in a
population of dividing cells. Using a proprietary chemistry,
the CytoTrack dye reacts with intracellular proteins and iseffectively retained in the cell without efflux. As cell divisionoccurs, the dye is successively halved and the different
generations can be determined based on the decrease influorescence intensity.

100

60
40
20

40

105

0

Cell-retaining
group
Esterasecleavage

100

Days 5 and 6
not collected

80
60
40

105

Days 5 and 6
not collected

80
60
40
20

20

Incubatewith cells

103
104
488 nm laser, 525/35 filter

CytoTrack Red 628/643 (DPBS)

CytoTrack Yellow 542/556 (media)100

 asily combine CytoTrack dyes with fluorescent proteinsEor antibody-labeled fluorophores for multicolor cell analysis

Fluorescence
blocker

103
104
405 nm laser, 460/22 filter

Normalized to mode■ ompatible with intracellular analysis using standard
C
formaldehyde-containing fixatives and saponin-based
permeabilization buffers

60

0
0

Normalized to mode■Dyes are directly added to cell culture media or buffer

80

20

0
■

Days 5 and 6
not collected000Maskedfluorophore

103
104
561 nm laser, 577/15 filter

105

0

103
104
640 nm laser, 670/30 filter

105

CFDA-SE (DPBS)

The nonfluorescent dye is cleared upon cell entry with the cellretaining group and then covalently binds to intracellular protein,allowing it to remain in the cell during division.
For More Information
Web: bio-rad.com/cytotrack
Download bulletin: #10033110
Request or download bulletin: 6524

Normalized to mode

100

Days 5 and 6
not collected

Gap between
days 0 and 1

80

Day 0: Far right peakDays 1–10: Peaks in between
Negative: Far left peak

60
40
20
0
0

103
104
488 nm laser, 525/35 filter

105

CytoTrack and CFDA-SE performance on the ZE5 cell analyzer.
1x CytoTrack solution in either cell culture media or buffer was added
to 1 x 106 Jurkat cells/ml. Cells were incubated in the dark at room
temperature for 15 min, then washed and resuspended with cell culture
media or buffer. Half were run on the ZE5 cell analyzer (day 0) and theother half left to grow. Each day, half the sample was removed to run
on the ZE5 cell analyzer and the cell culture was replenished. DPBS,
Dulbecco’s phosphate buffered saline.

Ordering Information

Catalog #Description

135120213512031351205

CytoTrack Blue 403/454
CytoTrack Green 511/525
CytoTrack Red 628/643

Maximum
Excitation,
nm
403
511
628

ZOE
ZE5
S3e
OptimalMaximum
Cell
Cell
Emission, Excitation Laser, Cell
Sorter Analyzer Imager
nm
nm
454
525
643

405
488
633 or 640

•
•
•

•
•
•

Price, U.S. $305.00305.00305.00Each kit includes 4 vials of dye and 1 vial DMSO for 200 cell proliferation tests.

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 57

Cell Biology Reagents
Cell Proliferation Assays

bio-rad.com/cellproliferationassay

CFDA-SE Cell Proliferation Assay
100

CFDA-SE cell proliferation assay provides carboxyfluoresceindiacetate succinimidyl ester (CFDA-SE) in a convenient andeasy-to-use format. CFDA-SE is a cell-permeable dye thatfluoresces after the acetate groups are cleaved by intracellularesterases, creating carboxyfluorescein succinimidyl ester(CFSE). As the succinimidyl ester group reacts with primaryamines of intracellular proteins, CFSE is retained in the celland is successively halved during cell divisions.

80

CFDA-SE excitation/
emission is 492/517 nm
in 0.1 M NaOH. Excitation
wavelength (—); emission
wavelength (—). AU,
absorbance unit.

40

450

Visualize up to 8 cell divisions

500
550
Wavelength, nm

600

650

Staining buffer inimize contamination and reduce dilution steps
Mwith small vial size

For More Information
Web: bio-rad.com/cytotrack

Culture media

100

100

80

80

Normalized to mode

■

60

20

Normalized to mode■

Intensity, AU

The following products are available on bio-rad.com.

60
40
20
0

60
40
20
0

102

103
104
105
10 6
CytoTrack Green 511/525

102

103

104
105
CFDA-SE

10 6

CytoTrack resolves up to ten cell divisions compared to eight for
CFDA-SE.

Ordering Information

Catalog #Description

1351201

CFDA-SE Cell Proliferation
Assay

Maximum
Excitation,
nm
492

ZOE
ZE5
S3e
OptimalMaximum
Cell
Cell
Cell
Emission, Excitation Laser,
Sorter Analyzer Imager
nm
nm
517

488

•

•

Price, U.S. $305.00Includes 5 x 100 µg vials 5(6)-carboxyfluorescein diacetate succinimidyl ester, CFDA-SE.

58 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagents
Cell Proliferation Assays

bio-rad.com/cellproliferationassay

Cell Proliferation Detection Antibodies

The following products are available on
bio-rad-antibodies.com.

105

The thymidine analog 5'-bromo-2'-deoxyuridine (BrdU) is a
popular reagent in various scientific disciplines ranging fromcancer to neuroscience research. The chemical isfrequently added to samples, for example mammalian celllines, and subsequently incorporated into newly synthesized
DNA during replication.

Our mouse anti-BrdU antibody, clone Bu20a (catalog#MCA2483), has been tested in flow cytometry andimmunocytochemistry. The product has been referenced in
12 publications. It is available in three different sizes (0.2 mg,
0.1 mg, and 20 µg) and is conjugated to FITC. In addition toflow cytometry and immunocytochemistry, the Bu20a clone
is recommended for immunohistochemistry.

BrdU FITC

S phase
10

3

102

G2

G1

0

0

0
25
0,
00

0

0,
00

0

15
0,
00

20

0

0

10
0,
00

–102
50
,0
0

To measure cell proliferation, the incorporated BrdU is
detected with anti-BrdU antibodies, for example the mouseanti-BrdU antibody clone Bu20a. However, to ensure thatthe antibody is able to bind to BrdU, DNA treatment prior
to antibody staining is required (Liboska et al. 2012). In theliterature, endonucleases such as deoxyribonuclease I(DNase I) and acids such as hydrochloric acid are frequently
used to cleave or denature the DNA to make incorporated
BrdU accessible (Liboska et al. 2012).

104

Propidium iodide

Staining profile of anti-BrdU antibody. BrdU-labeled human
lymphoma cells were stained with mouse anti-BrdU antibody, clone
BU20a, at a 1/100 dilution overnight at 4°C. A secondary antibody,
FITC-conjugated goat anti-mouse IgG (H/L) (#STAR117F) was used
at a 1/50 dilution. Total DNA staining was performed with ReadiDrop
propidium iodide (#1351101). FITC, fluorescein isothiocyanate.

Detection of BrdU is commonly performed in combination
with total DNA staining. When performing cell cycle analysis,
propidium iodide tends to be the DNA stain of choice.
Ki67, proliferating cell nuclear antigen (PCNA), andminichromosome maintenance protein 2 (MCM2) are key
proteins for regulating cellular growth and proliferation.

Ordering Information
Catalog #Description

Clone

Species
Reactivity

MCA2483

BrdU

Bu20a

−

AHP2405
HCA053

BrdU
Ki67

−
2531

HCA006

Ki67

VMA00259
MCA1859
VMA00018

MCM2 PrecisionAb
Antibody
MCM2
PCNA PrecisionAb

AbD2531(Fab)
OT13B6

MCA1558

PCNA

PC10

−
Bovine, dog,
human
Bovine, dog,human
Human, mouse,
rat
Human
Human, mouse,
rat
Broad

AHP1419

PCNA Polyclonal−

CRCT2.1−

Bovine, dog,
human, mouse, rat

Price, U.S. $Applications

Host

Formats

FC,* IF/ICC,
IHC-F, IHC-P*
F,* IF/ICC, IF,* P
E, IHC-F,
IHC-P,* WB
E, IF/ICC, IHC-F,
IHC-P,* WB
WB

Mouse

Pur., FITC

305.00

RabbitPur.
HuCAL® Pur.

305.00

HuCAL

Pur.

305.00

Mouse

Pur.

305.00

IHC-P,* WB
WB

Mouse
Mouse

Pur.
Pur.

305.00305.00

FC,* IHC-F,
IHC-P, IP, WB
E, IHC-P, WB

Mouse

Pur., FITC,
HRP
Pur.

305.00

Rabbit305.00Applications: E, ELISA; FC, flow cytometry; IF/ICC, immunofluorescence/immunocytochemistry; IHC-F, immunohistology-frozen;
IHC-P, immunohistology-paraffin; IP, immunoprecipitation; WB, western blotting.
Formats: FITC, fluorescein isothiocyanate; HRP, horseradish peroxidase; Pur., purified.
* Requires special treatment. Refer to product datasheet.

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 59

Cell Biology Reagents
Cell Proliferation Assays

bio-rad.com/brduabs

Cell Proliferation Detection Antibodies
Anti-BrdU Antibodies

Our mouse anti-BrdU antibody, clone Bu20a (catalog#MCA2483), has been tested in flow cytometry andimmunocytochemistry. The product has been referenced
in 12 publications. It is available in three different sizes(0.2 mg, 0.1 mg, and 20 µg) and is conjugated to FITC. In
addition to flow cytometry and immunocytochemistry, theBu20a clone is recommended for immunohistochemistry.
To ensure that the antibody is able to bind to BrdU, DNA
treatment prior to antibody staining is required (Liboska
et al. 2012). In the literature, endonucleases such as
deoxyribonuclease I (DNase I) and acids such as hydrochloric
acid are frequently used to cleave or denature the DNA to
make incorporated BrdU accessible (Liboska et al. 2012).

105

104
BrdU: DyLight 488

The thymidine analog 5’-bromo-2’-deoxyuridine (BrdU) is
a popular reagent in various scientific disciplines ranging
from cancer to neuroscience research. The chemical isfrequently added to samples, for example mammaliancell lines, and subsequently incorporated into newly
synthesized DNA during replication. To measure cellproliferation, the incorporated BrdU is detected with
anti-BrdU antibodies, for example the mouse antiBrdU antibody clone Bu20a and our highly validated
PrecisionAb Anti-BrdU Antibodies. These PrecisionAb
Anti-BrdU Antibodies are tested in multiple applications
and for cross-reactivity with thymidine and other thymidine
analogs. Detailed protocols for various immunodetection
applications provide guidance to optimize their use.

S Phase
10

3

102
G1

101

G2

10 0
0

50K

100K
150K
Propridium Iodide

200K

Staining profile of PrecisionAb Rat Anti-BrdU Antibody, clone
RF04-2. Jurkat cells were treated with 100 μM BrdU and stained with
rat anti-BrdU antibody clone RF04-2 (MCA6143). As a secondary
antibody, rabbit F(ab’)2 anti-rat IgG: DyLight488 conjugated antibody(STAR16D488GA) was used at a 1/400 dilution. ReadiDrop propidium
iodide was used to stain total DNA.

Check out the wide range of Anti-BrdU antibodies below(and the appropriate application use) and find the right one
for your experimental needs.
Detection of BrdU is commonly performed in combination
with total DNA staining. When performing cell cycle
analysis, propidium iodide tends to be the DNA stain of
choice. Ki67, proliferating cell nuclear antigen (PCNA), andminichromosome maintenance protein 2 (MCM2) are key
proteins for regulating cellular growth and proliferation.
For More Information
Web: bio-rad.com/brduabs

Ordering Information
Catalog #Description

HCA322

Human anti BrdU

HCA320

Human anti BrdU

HCA323

Human anti BrdU

HCA321

Human anti BrdU

MCA6143
MCA6144

Rat anti BrdU
Rat anti BrdU

60 | Flow Cytometry

Price, U.S. $305.00305.00In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagents
Cell Viability Assays

bio-rad.com/vivafix

Cell Viability Assays
VivaFix Cell Viability Assay
The following products are available on bio-rad.com.

A

B

VivaFix cell viability assays provide a sensitive method for
determining the viability of live or fixed mammalian cellsby flow cytometry, cell sorting, and microscopy.
Using proprietary dyes that covalently bind to primaryamines, VivaFix cell viability assays provide at least a
100-fold difference in fluorescence intensity between live
and dead cells. The brightness of VivaFix dyes ispreserved upon treatment with fixative agents.
■

100-fold discrimination between dead and live cells■

Compatible with fixed and unfixed cells■

8 different excitation/emission wavelength combinations

A

VivaFix cell viability assay chemistry. A, VivaFix dyes bind to the cellsurface primary amines of viable cells; B, in dead cells, where the plasma
membrane is compromised, VivaFix dyes are able to permeate the celland also react with intracellular primary amines. As a result, a greaternumber of fluorophores is associated with dead cells and at least a
100-fold difference in fluorescence intensity is measured between the live
and the dead cells, thereby allowing an easier discrimination between thetwo populations.
A
256

1,000,000
Dead
cells
Live
cells

600,000

Dead
cells

192
SSC area

SSC area

800,000

400,000

Live
cells

128

64
200,000

0

0
10 0

101

102

103

104

VivaFix 498/521

B

10 0

B

SSC area

SSC area

400,000

104

105

104

105

Dead
cells

192

Live
cells

103

256

Dead
cells

600,000

102

VivaFix 353/442

1,000,000

800,000

101

Live
cells

128

64
200,000

0

0
10 0

101

102

103

104

VivaFix 498/521

Assessment of cell viability using VivaFix and the S3e cell sorter.
Jurkat cells were stained with the VivaFix 498/521 dye, fixed with
3.7% formaldehyde (A) or not fixed (B), and analyzed with the S3e cell
sorter. SSC, side scatter.

For More Information
Web: bio-rad.com/vivafix

In the U.S., call toll free at 1-800-424-6723

10 0

101

102

103

VivaFix 353/442

Assessment of cell viability using VivaFix and the ZE5 cell analyzer.
Jurkat cells were heat shocked at 65°C for 5 min, then washed andsuspended at 2 x 106 cells/ml in phosphate buffered saline. Cells wereincubated with VivaFix 353/442 at 37°C for 30 min in the dark. A, halfof the cells fixed in 1% paraformaldehyde; B, half of the cells not fixed.
Identical gates for all samples were used during analysis on the ZE5cell analyzer. SSC, side scatter.

Flow Cytometry | 61

Cell Biology Reagents
Cell Viability Assays

bio-rad.com/vivafix

Ordering Information

Catalog #Description

1351111135111213511131351115135111613511171351118

VivaFix 353/442
VivaFix 410/450
VivaFix 408/512
VivaFix 498/521
VivaFix 547/573
VivaFix 583/603
VivaFix 649/660

Maximum
Excitation,
nm

Price, U.S. $

ZOE
ZE5
S3e
OptimalMaximum
Cell
Cell
Emission, Excitation Laser, Cell
Sorter Analyzer Imager
nm
nm

353
410
408
498
547
583
649

442
450
512
521
573
603
660

355
405
405
488
561
561
640

•
•
•
•
•
•
•

•
•
•
•
•
•

•

305.00•••305.00305.00305.00Each VivaFix assay includes 4 x 50 test vials and 250 µl of DMSO.

ReadiDrop Cell Viability Assays
The following products are available on bio-rad.com.
Designed for ease of use in cell sorting and flow cytometry applications, ReadiDrop cell viability assays remove thetraditional manual weighing, pipetting, and dilution steps required with commonly available cell viability dyes. Simply add
one or two drops to assess the health of your cells.
ReadiDrop Propidium Iodide
Cell impermeant nucleic acid–binding dye that binds todouble-stranded DNA/RNA■

ReadiDrop 7-AAD
Cell impermeant DNA binding dye with a high affinity
for GC-rich regions of double-stranded DNA■■

Ready-to-use formulation in PBS without preservatives■

Ideal for cell sorting and flow cytometry■

Stable at room temperature■

Stable at room temperature

Excitation/emission = 538/617 nm+ DNA in pH 7.0 buffer

100
80
60
40
20
0

120
Intensity, absorbance unit (AU)Intensity, absorbance unit (AU)120

Excitation/emission = 548/648 nm+ DNA in pH 7.0 buffer

100
80
60
40
20
0

400

500

600
Wavelength, nm

700

400

500

600
Wavelength, nm

700

800

Excitation and emission curves for propidium iodide and 7-AAD.

62 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagents
Cell Viability Assays

bio-rad.com/readidrop1

ReadiDrop 7-AAD

ReadiDrop propidium iodide
Live
cells

Live
cells

Dead
cells
84

Count

Count

105

70

56

28

35

0

0
10 0

101

102
103
488 nm laser, 593/52 filter

Live
cells

104

10 0

101
102
103
488 nm laser, 692/80 filter

Live
cells

Dead
cells

104

Dead
cells

66

Count

123

Count

Dead
cells

82

44

22

41

0

0
10 0

101

102
103
561 nm laser, 615/24 filter

10 0

104

101
102
103
561 nm laser, 640/20 filter

104

Assessment of cell viability using ReadiDrop reagents and the ZE5 cell analyzer. Jurkat cells were heat shocked at 65°C for 5 min. Cells were
washed and resuspended at 2 x 106 cells/ml in phosphate buffered saline (PBS). ReadiDrop propidium iodide or 7-AAD (20 μl) was added to
0.5 ml cells and incubated for 15 min at room temperature in the dark. Cells were washed once in PBS prior to acquisition on the ZE5 cell analyzer.
For More Information
Web: bio-rad.com/readidrop1
Download bulletin: #10049240
Request or download bulletin: 6522

Ordering Information

Catalog #Description

1351101
1351102

Propidium Iodide
7-AAD

Maximum
Excitation,
nm
538
548

ZOE
ZE5
S3e
OptimalMaximum
Cell
Cell
Emission, Excitation Laser, Cell
Sorter Analyzer Imager
nm
nm
617
648

561 or 488
561 or 488

•
•

•
•

Price, U.S. $305.00305.00Each assay includes 3 x 3 ml bottles of ready-to-use reagent suspended in PBS for dead cell exclusion in cell sortingand flow cytometry applications.

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 63

Cell Biology Reagents
Flow Cytometry Calibration and Validation Reagentsbio-rad.com/calibration

Flow Cytometry Calibration and Validation ReagentsStandards and Stabilizers

The following products are available on
bio-rad-antibodies.com.
Flow Cytometry Absolute Count Standard
Flow Cytometry Absolute Count Standard is a suspensionof microspheres that are approximately the size of human
lymphocytes (7–9 µm) and are presented at a known
concentration. They are internally labeled with multiple
fluorochromes and the combination of dyes allows thebeads to be excited by a common argon laser (488 nm)
and emit in the three standard channels of a flow cytometer(FL1, FL2, and FL3).

RPE Reference Standard
RPE reference standard consists of one vial of
microspheres that are uniform in both size andfluorescence and are approximately the size of human
lymphocytes (7–9 µm). The excitation and emission spectraof the microspheres match those of cell samples labeledwith RPE. It is used to provide qualitative data whencompared to a sample but can also be used to calibrate
the flow cytometer.
Protein Stabilizer
Protein stabilizer stabilizes antibodies and other proteinsor biomolecules in solution. It may be used to stabilize
ready-to-use prediluted standards and controls used in
immunoassays.
For More Information
Web: bio-rad.com/calibration

Ordering Information
Catalog #Description

FCSC580
FCSC899B
BUF069A

Flow Cytometry Absolute Count Standard, 1 bottle containing 10 ml of microspheres, 20 tests
RPE Reference Standard, 1 x 5 ml bottle of labeled microbeads for 100 tests
Protein Stabilizer, 100 ml of ready-to-use liquid

64 | Flow Cytometry

Price, U.S. $78.00

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagentsbio-rad.com/calibration

Flow Cytometry Calibration and Validation Reagents

Calibration ReagentsThe following products are available on
bio-rad-antibodies.com.
Quantum kits are used for the quantitation of fluorescenceintensity in molecules of equivalent soluble fluorochrome(MESF) units. When used in conjunction with Simply
Cellular microspheres, this kit also allows quantitation of
antibody binding capacity (ABC). The fluorescence intensityof each of the populations in the kit has been calibrated
against solutions of laser-grade fluorescent dye in units of
MESF dye per microsphere. The Certified Blank population
is used to measure the fluorescence detection threshold of
the instrument.
■

■

■

■

 uantitation of fluorescence intensity of samples
Q
in terms of MESF
 etermination of instrument fluorescenceDdetection threshold
Determination of instrument linearity
 ata comparison over time and between
Dmultiple instruments

Quantum FITC-5 MESF is composed of five calibrated
fluorescent populations in one tube, each with different levelsof FITC fluorescence intensity, and one blank populationof uniform microspheres that are approximately the sizeof human lymphocytes (7–9 µm). Quantum R-PE MESF is
composed of four calibrated fluorescent populations, withdifferent levels of RPE fluorescence intensity.
Quantum Simply Cellular mouse IgG is composed of four
coated populations and one blank population of uniform
microspheres that are approximately the size of human
lymphocytes (7–9 µm). Each of the four sets is coated withgoat anti-mouse IgG (Fc-specific) with different calibrated
antibody binding capacities (ABC) for mouse monoclonal
antibodies. The blank population has no specific binding
capacity for mouse IgG. When the bead populations arelabeled in the same manner as the cells to be analyzed,
they provide a means for constructing a QuickCalcalibration curve (ABC values vs. fluorescence intensity),
from which samples may be “read.” Cellular antigen
expression may thus be quantitated in ABC units.
For More Information
Web: bio-rad.com/calibration

Ordering Information
Catalog #Description

FCSC555A

Quantum FITC-5 MESF, 1 x 1 ml bottle of unlabeled microbeads, 5 x 1 ml bottles of labeled
microbeads for 20 tests
Quantum FITC-5 MESF, 1 x 5 ml bottle of unlabeled microbeads, 5 x 5 ml bottles of labeled
microbeads for 100 tests
Quantum FITC-5 MESF (Pre-Mixed), 1 x 1 ml bottle of unlabeled microbeads, 5 x 1 ml bottlesof labeled microbeads for 20 tests
Quantum FITC-5 MESF (Pre-Mixed), 1 x 5 ml bottle of unlabeled microbeads, 5 x 5 ml bottlesof labeled microbeads for 100 tests
Quantum R-PE MESF Medium Level, 1 x 1 ml bottle of unlabeled microbeads, 4 x 1 ml bottlesof labeled microbeads for 20 tests
Quantum R-PE MESF Medium Level, 1 x 5 ml bottle of unlabeled microbeads, 4 x 5 ml bottlesof labeled microbeads for 100 tests
Quantum Simply Cellular Mouse IgG, 1 x 1 ml bottle of unlabeled microbeads, 4 x 1 ml bottlesof labeled microbeads for 20 tests
Quantum Simply Cellular Mouse IgG, 1 x 5 ml bottle of unlabeled microbeads, 4 x 5 ml bottlesof labeled microbeads for 100 tests
Quantum Simply Cellular Mouse IgG, 2 x 7 ml bottle of unlabeled microbeads, 8 x 7 ml bottlesof labeled microbeads for 280 tests

FCSC555BFCSC555PAFCSC555PBFCSC827AFCSC827B
FCSC815AFCSC815B
FCSC815C

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
78.00

78.00

Flow Cytometry | 65

Cell Biology Reagents
Flow Cytometry General Reagentsbio-rad.com/flowcytometryreagents

Flow Cytometry General Reagents
Accessory ReagentsThe following products are available on
bio-rad-antibodies.com.
Human Seroblock
Human Fc receptors are expressed on a variety of immune
cell types including monocytes, macrophages, B cells,
granulocytes, and dendritic cells. Cells that express Fc
receptors can produce false positive immunofluorescent
staining due to the Fc receptors binding Ig. Human
seroblock is designed to prevent such nonspecific staining
without interfering with appropriate target staining. Human
seroblock is compatible with anti-human antibodies
targeting Fc receptors in flow cytometry.
Mouse Seroblock FcR
Mouse seroblock FcR is a rat antibody that recognizes
mouse CD16 and CD32 cell surface proteins also known
as FcRgIII and FcRgII, respectively. The function of these
proteins is to bind IgG molecules via their Fc regions as partof the adaptive immune response. CD16 and/or CD32 areexpressed by a wide variety of cells, including monocytes,
macrophages, B lymphocytes, granulocytes, NK cells,
dendritic cells, and some activated T lymphocytes. The
expression of CD16/CD32 antigens can lead to nonspecificbinding of test monoclonal antibodies in staining
procedures, resulting in high background staining on a wide
range of cells. This nonspecific binding may be blocked by
pre-incubation of target cells with mouse seroblock FcR,
resulting in cleaner staining.
Neutralite Avidin:Cy5 or RPE
Neutralite avidin is a deglycosylated version of egg whiteavidin that retains its affinity for biotin but has a near-neutral
pH that minimizes nonspecific interactions.

66 | Flow Cytometry

Streptavidin:APC or FITC
Streptavidin is designed as a secondary reagent for use
in flow cytometry in conjunction with biotinylated primary
antibodies. Its conjugates show negligible nonspecificbinding to nonbiotinylated macromolecules, and therefore
produce very low backgrounds.
Leucoperm Reagent
Leucoperm reagents allow intracellular antigen analysis
with the same ease as surface antigens. The only
prerequisite is the availability of suitable antibody
conjugates. Most commercially available monoclonal
antibody conjugates can be used with Leucoperm
reagents. Some determinants are sensitive, however, tothe fixation step involved. This and the optimal fixation
time may have to be determined experimentally for eachantibody conjugate.
Staining Buffer
Bio-Rad’s staining buffer contains bovine calf serum. It has
been optimized for staining both live and fixed cells with
antibodies. It is suitable for use with both conjugated andunconjugated antibodies and can be used as an antibody
dilution media and a wash buffer. The addition of bovine
calf serum reduces background staining of target cells.
The staining buffer also contains sodium azide to minimize
receptor capping.
Fixation Buffer
The fixation buffer is a paraformaldehyde-based fixative thatis suitable for use with most cell types. Paraformaldehydecontaining fixation buffer is essential for intracellular staining
prior to permeabilization.
Intracellular Permeabilization/Wash BufferThis permeabilization and wash buffer is for intracellular
staining. Access to intracellular targets, such as cytokinesand cytoplasmic proteins, requires permeabilization of thecell membrane after fixation. This buffer has minimal effecton cell morphology. The buffer is supplied as a 10x
solution for dilution in water. It can also be used as awash buffer during intracellular staining to help minimize
background staining.

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagentsbio-rad.com/flowcytometryreagentsProtein Transport Inhibitors
Protein transport inhibitors can be used to enhance
intracellular cytokine staining. As cytokines and chemokines
are usually secreted, use of these inhibitors traps theproteins within the cell, leading to a buildup of cytokines in
the Golgi apparatus and endoplasmic reticulum. The choiceof and culture conditions for these inhibitors may need to
be optimized depending on the species and cytokines
being stained. Monensin acts by interfering with the Na+/H+
transport in Golgi apparatus transmembranes. Brefeldin A
acts by redistributing proteins from the Golgi apparatus to
the endoplasmic reticulum.

Flow Cytometry General Reagents

Activation Reagents
Bio-Rad offers a cell activation reagent with the proteintransport inhibitor Brefeldin A. It contains optimized
concentrations of phorbol 12-myristate-13-acetate (PMA)
and ionomycin, which can be used to activate your cellsof interest. Available as a 500x solution in DMSO, it issuitable for activation of cells and stimulatingcytokine secretion.
For More Information
Web: bio-rad.com/flowcytometryreagents

Red Cell Lysing Buffer
The red cell lysing buffer, erythrolyse, is available as a 10x
solution that is stable for 1 year at room temperature. It is
suitable for the lysing of red blood cells in human, rat, andmouse blood.

Ordering Information
Catalog #Description

BUF070A
BUF070B
BUF041ABUF041B
STAR119
STAR2B
BUF09
BUF09B
BUF09CBUF073
BUF071
BUF074
BUF075BUF04B
BUF04CBUF036A
BUF077A

Human Seroblock, includes a solution of buffer and blocking agents for 50 tests
Human Seroblock, includes a solution of buffer and blocking agents for 200 tests
Mouse Seroblock FcR, includes 0.1 mg of purified IgG
Mouse Seroblock FcR, includes 0.5 mg of purified IgG
Streptavidin:APC, includes lyophilized product for 100 tests
Streptavidin:FITC, includes 1 mg of streptavidin:FITC at 1 mg/ml
Leucoperm Reagent, fixation and permeabilization mediums for 50 tests
Leucoperm Reagent, fixation and permeabilization mediums for 250 tests
Leucoperm Reagent, fixation and permeabilization mediums for 1,000 tests
Staining Buffer, 500 ml
Fixation Buffer, 100 ml
Monensin, 1000x solution, 1 ml
Brefeldin A, 1000x solution, 1 ml
Red Cell Lysing Buffer, 10x solution, 100 tests/20 ml
Red Cell Lysing Buffer, 10x solution, 500 tests/100 ml
Phosphate Buffered Saline (PBS), 10x, 1 L
Cell Stimulation Reagent (with Brefeldin A), 500x solution, 0.1 ml

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
78.00

78.00

Flow Cytometry | 67

Cell Biology Reagents
Nuclear Staining Dyes

bio-rad.com/PureBluHoechst33342

Nuclear Staining Dyes
See Also

VivaFix cell
viability assay:
page 61.
Cell proliferation
assays: page 56.

PureBlu Hoechst 33342 Nuclear Staining Dye
The following products are available on bio-rad.com.
PureBlu Hoechst 33342 nuclear staining dye is a highly
pure formulation of Hoechst 33342 fluorescent dye.
It is available in a user-friendly format that allows easy
preparation of the working solution without any weighing
step and with a single dilution after resuspension.
PureBlu Hoechst 33342 dye permeates cell membranes
and binds double-stranded DNA, which enables
identification of nuclei within cells. It has a maximum
excitation wavelength of 350 nm in the ultraviolet range
and can be optimally detected in the blue channel witha maximum emission wavelength of 461 nm.
Product features include:
■

■

■

100
80
Normalized intensity

ReadiLink antibody
labeling kits:
page 52.

60
40
20
0

300

400

500

600

700

Wavelength, nm

Excitation and emission spectra of PureBlu Hoechst 33342 dye.
For More Information
Web: bio-rad.com/PureBluHoechst33342
Download bulletin: #10043283
Request or download bulletin: 6607

Easy to reconstitute without any weighing step
Optimal for live cell staining
Compatible with multicolor experiments

Ordering Information

Catalog #Description

1351304

PureBlu Hoechst 33342

Maximum
Excitation,nm

Maximum
Emission,nm

OptimalExcitation
Laser, nm

ZE5
Cell
Analyzer

ZOE
Cell
Imager

350

461

355

•

•

Price, U.S. $305.00Includes 5 x 56 µg vials of Hoechst 33342 powder.

PureBlu DAPI Nuclear Staining Dye
The following products are available on bio-rad.com.
PureBlu DAPI nuclear staining dye is a highly pure formulation of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)
fluorescent dye. It is available in a user-friendly format that allows easy preparation of the working solution without any
weighing step and with a single dilution after resuspension. PureBlu DAPI dye permeates cell membranes and binds doublestranded DNA, which enables identification of nuclei within cells. It has a maximum excitation wavelength of 359 nm in theultraviolet range and can be optimally detected in the blue channel with a maximum emission wavelength of 461 nm.
Product features include:■■■

Easy to reconstitute without any weighing step
Optimal for fixed cell staining
Compatible with multicolor experiments

For More Information
Web: bio-rad.com/PureBluDAPI
Download bulletin: #10043282
Request or download bulletin: 6607

68 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Cell Biology Reagents
Nuclear Staining Dyes

bio-rad.com/PureBluDAPI

A

120

Normalized intensity

100
80
60
40
20
0
300

350

400

450

500

550		

600

Wavelength, nm

Excitation and emission spectra of PureBlu DAPI dye.

256
Dead
cells

SSC area

192

B

Live
cells

128

64

0
10 0

101

102

103

104

105

355 nm laser, 447/60 filter

Assessment of cell viability using PureBlu DAPI and the ZE5 cell
analyzer. Jurkat cells were heat shocked at 65°C for 5 min. Cells
were washed and resuspended at 2 x 106 cells/ml in phosphatebuffered saline. 100x PureBlu DAPI (5 μl) was added to 0.5 ml cells
and incubated for 15 min at room temperature in the dark. Cells werethen run on the ZE5 cell analyzer. SSC, side scatter.

PureBlu dyes are the optimal choice for staining the DNA of
eukaryotic cells. HeLa cells were transfected with a green fluorescentprotein (GFP)–expressing plasmid DNA, stained, and viewed in a sixwell dish using a ZOE fluorescent cell imager. A, cells counterstainedwith PureBlu DAPI nuclear staining dye; B, merged view of cells
stained with PureBlu DAPI and GFP.

Ordering Information

Catalog #Description

1351303

PureBlu DAPI

Maximum
Excitation, nm

Maximum
Emission,nm

OptimalExcitation Laser,
nm

ZE5
Cell
Analyzer

ZOE
Cell
Imager

359

461

355

•

•

Includes 5 x 50 µg vials of DAPI powder.

In the U.S., call toll free at 1-800-424-6723

Flow Cytometry | 69

Regulated Cell Death Reagents
Apoptosis Reagentsbio-rad.com/apoptosis

Regulated Cell Death Reagents
Cell death is a crucial step in organism development, tissue homeostasis, and host defense. There are many mechanisms ofregulated cell death (RCD) such as apoptosis, pyroptosis, necroptosis, and autophagy, where signal transduction molecules
are not only initiated but also involved in the propagation of cell death.
Initiation of RCD can result from tissue-turnover, known as programmed cell death (PCD), or disruption of the intercellular
or extracellular environment (stress driven RCD). Regulated cell death is involved in immunological processes including
elimination of infected cells, removal of effector immune cells, and lymphocyte development.
For More Information
Web: bio-rad.com/rcd

Apoptosis Reagents
Apoptosis, one of the main forms of programmed cell death, is essential for removing damaged or old cells and is involvedin organism development, tissue homeostasis, and host defense of infected or transformed cells. Reagents to common
apoptosis markers allow determination of cellular apoptosis levels. Activation of the caspase cascade initiates this process
through either the intrinsic or extrinsic pathway.

Annexin V Kits

The following products are available on
bio-rad-antibodies.com.
Early apoptotic cells can be detected with the help ofannexin V, a protein encoded by the ANXA5 gene, whichacts as an anticoagulant. Annexin V, like other membersof the annexin protein family, binds to specific membraneproteins. During the early stages of apoptosis, theplasma membrane is rearranged, resulting in changes
to the cell membrane’s phospholipid asymmetry. Oneof the effects of these rearrangements is the display ofphosphatidylserine (PS) at the outer plasma membrane.
In healthy, quiescent cells, PS is exclusively locatedon the inner membrane and therefore the exposure of

PS is an indication of early apoptosis (Fadeel and Xue,2009). Annexin V has a high binding affinity for PS in thepresence of Ca2+ and is therefore a commonly used toolfor detecting apoptotic cells.
■■■

 pplicable for multicolor flow cytometry experiments;Aavailable as conjugates, including PE, and APC
Includes the viability dye propidium iodide for distinction
between apoptotic/secondary necrotic and apoptotic cells
 abeled annexin V, included in the kits, has beenL
conjugated such that the native phospholipid binding
properties of annexin V remain unaltered

For More Information
Web: bio-rad.com/apoptosis

Ordering Information
Catalog #Description

ANNEX50PE

Annexin V:PE Assay Kit, 50 tests annexin V:PE, 7-AAD viability staining solution, 10x binding buffer

Price, U.S. $ANNEX200PE

Annexin V:PE Assay Kit, 200 tests annexin V:PE, 7-AAD viability staining solution, 10x binding buffer

ANNEX50APC Annexin V:APC Assay Kit, 50 tests annexin V:APC, propidium iodide staining solution, 10x binding buffer
ANNEX200APC Annexin V:APC Assay Kit, 200 tests annexin V:APC, propidium iodide staining solution, 10x binding buffer 78.00

70 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Regulated Cell Death Reagents
Apoptosis Reagentsbio-rad.com/apoptosis

Caspase Kits

The following products are available on
bio-rad-antibodies.com.
Active caspases in apoptotic cells can be analyzed using
FLICA kits, which offer:■■■

Unique cell-permeable and non-cytotoxic FLICA reagent
 uick and easy method suitable for flow cytometry,Q
fluorescence microscopy, or a plate reader
Choice of 4 fluorophores, from green to far red

FLICA caspase apoptosis detection kits allow analysisof active caspases in whole, living cells. The uniquecell-permeable and non-cytotoxic reagent, called
fluorochrome inhibitor of caspases (FLICA), contains a
caspase inhibitor sequence (such as VAD) linked to agreen (carboxyfluorescein, FAM) or red (sulforhodamine,SR) fluorescent probe. As FLICA binds only to the active
caspase, there is no interference from pro-caspases orinactive forms of the enzyme. The remaining fluorescentsignal gives a direct measure of the amount of active
caspase in the whole living cell. Cellular analysis may be
performed with a fluorescence microscope, plate reader, or
flow cytometer.

FLICA caspase kits are suitable for cells in suspension andadherent cells, thin tissue sections, and thin frozen sections(but not fixed or paraffin-embedded tissues) from many
species, including mammals, insects, and yeast. Various
cell types, including HeLa, primary neurons, macrophages,
and lymphocytes, have been successfully studied withthese kits.
FAM FLICA fluoresces green and the SR FLICA fluoresces
red. The latter is useful for studying caspase activity in
GFP-transfected cell lines and for dual-color experimentswith other green reagents. For general apoptosis
detection, poly caspase kits (caspase 1, 3, 4, 5, 6, 7, 8, 9) arerecommended, as they will react with all active caspases.
Alternatively, individual caspase activity may be studied
with the single FLICA caspase kits. The Magic Red
reagent contains a caspase inhibitor sequence (DEVD)2
linked to a red (cresyl violet) fluorescent probe.
The FLICA 660 caspase kits use a target sequence
sandwiched between a far red fluorescent 660 dye and afluoromethylketone to make a quick and flexible method toanalyze active caspases.
For More Information
Web: bio-rad.com/apoptosis

Ordering Information
Catalog #Description

ICT097
ICT098
ICT9122
ICT918
ICT093
ICT094
ICT935
ICT936
ICT9125
ICT095
ICT096
ICT099
ICT910
ICT912
ICT913
ICT960
ICT961
ICT923
ICT091
ICT092
ICT916
ICT917
ICT9120

FAM FLICA Caspase-1 Kit, 25 tests
FAM FLICA Caspase-1 Kit, 100 tests
FLICA 660 Caspase-1 Kit, 25–50 tests
FAM FLICA Caspase-2 Kit, 25 tests
FAM FLICA Caspase-3/7 Kit, 25 tests
FAM FLICA Caspase-3/7 Kit, 100 tests
Magic Red Caspase-3/7 Kit, 25 tests
Magic Red Caspase-3/7 Kit, 100 tests
FLICA 660 Caspase-3/7 Kit, 25–50 tests
FAM FLICA Caspase-6 Kit, 25 tests
FAM FLICA Caspase-6 Kit, 100 tests
FAM FLICA Caspase-8 Kit, 25 tests
FAM FLICA Caspase-8 Kit, 100 tests
FAM FLICA Caspase-9 Kit, 25 tests
FAM FLICA Caspase-9 Kit, 100 tests
SR FLICA Caspase-9 Kit, 25 tests
SR FLICA Caspase-9 Kit, 100 tests
FAM FLICA Caspase-10 Kit, 100 tests
FAM FLICA Poly Caspase Kit, 25 tests
FAM FLICA Poly Caspase Kit, 100 tests
SR FLICA Poly Caspase Kit, 25 tests
SR FLICA Poly Caspase Kit, 100 tests
FLICA 660 Poly Caspase Kit, 25–50 tests

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $78.00

Flow Cytometry | 71

Regulated Cell Death Reagents
Apoptosis Reagentsbio-rad.com/apoptosis

Mitochondrial Membrane Potential Kits

The following products are available on
bio-rad-antibodies.com.
An early indication of apoptosis involves a collapse in
the electrochemical gradient across the mitochondrial
membrane. This is mediated by the opening of
permeability transition (PT) pores and is known as the
mitochondrial PT event. It is measured by the change
in the mitochondrial membrane potential. Loss of
mitochondrial membrane potential can be detected bythree fluorescent cationic dyes: 5,5',6,6'-tetrachloro1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide(JC-1), tetramethylrhodamine ethyl ester (TMRE), andtetramethylrhodamine methyl ester (TMRM).
These dyes are lipophilic and have a delocalized
positive charge, which enables them to enter cells andnegatively charged healthy mitochondria. In nonapoptotic

mitochondria, the dyes aggregate and will fluoresce brightorange/red. As apoptosis occurs and the mitochondrial
membrane potential collapses, the dyes disperse
throughout the cell and the fluorescence either diminishes(MitoPT TMRE kit) or changes color (MitoPT JC-1 kit).
This allows an easy distinction between apoptotic andnonapoptotic fluorescent cells, which can be readwith a flow cytometer, fluorescence microscope, or afluorescence plate reader using black microtiter plates.
■■■ uick and easy staining method clearly differentiatesQ
nonapoptotic and apoptotic cells
 itoPT TMRE kit is ideal for single-cellMmultiparametric analyses
 uitable for flow cytometry, fluorescence microscopy,Sor a plate reader

For More Information
Web: bio-rad.com/apoptosis

Ordering Information
Catalog #Description

Price, U.S. $ICT946
ICT943
ICT944

Mitochondrial Membrane Potential: MitoPT TMRE Kit, 500 tests
Mitochondrial Permeability Transition: MitoPT JC-1 Kit, 100 tests
Mitochondrial Permeability Transition: MitoPT JC-1 Kit, 400 tests

78.00

Real-Time Apoptosis Fluorescent Microscopy Kit■

The following products are available on
bio-rad-antibodies.com.
Compatible with conventional fluorescence microscopes
and the ZOE fluorescent cell imager, IANBD (excitation
maximum 488 nm, emission maximum 530 nm) can beeasily detected by FITC filter sets.

Ideal for performing time-lapse live cell imaging
experiments■

Visualize apoptosis events in real time■

Perfect for comparing mutant and wild type strains■

Observe transient and reversible apoptotic events■

 o washing steps or special buffers required — just add
N
the pSIVA probe and start imaging■

Differentiate apoptotic from necrotic/dead cells■

The kit includes the propidium iodide viability dye

For More Information
Web: bio-rad.com/apoptosis

Ordering Information
Catalog #Description

APO004pSIVA Real-Time Apoptosis Fluorescent Microscopy Kit, 1 kit

72 | Flow Cytometry

Price, U.S. $In the U.S., call toll free at 1-800-424-6723

Regulated Cell Death Reagents
Apoptosis Reagentsbio-rad.com/apoptosis

Serine Protease Detection Kits

The following products are available on
bio-rad-antibodies.com.
FAM FLISP reagents are noncytotoxic, cell permeant green
fluorescent inhibitors that covalently bind to active serine
protease enzymes. Simply add FLISP directly to cells in
culture, incubate, and wash to detect active chymotrypsinlike enzyme inside the cell. FLISP will covalently bind an
active chymotrypsin-like enzyme and be retained within
the cell, whereas unbound reagent will be able to diffuse
out. Cells containing lower levels of active enzyme will bindless FLISP and therefore be less fluorescent after thewash steps.
Serine proteases, such as trypsin and chymotrypsin, aredefined by the presence of a serine residue at the active
center of the enzyme, which participates in the formationof an intermediate ester to transiently form an acyl-enzyme
complex. Activation of serine proteases plays major roles

in several different cellular functions, including apoptosis,
tumor malignancy, and as diagnostic and prognostic
indicators of breast, head, and neck carcinomas. Serine
protease activation is also thought to play a role in
infections and transplant rejection. There is increased
serine protease activity in apoptotic cells downstream of
caspase activation, and this may complement caspasemediated cell death.
Counterstaining with a viability dye such as propidium
iodide (contained in the kit) allows identification of necrotic
cells by flow cytometry. Nuclear morphology may beobserved by fluorescence microscopy using Hoechst(contained in the kit).
The kits are available with either a chloromethyl ketone(CMK) or diphenyl 1-(N-peptidylamino) alkanephosphonate(DAP) reactive group–containing compound with different
inhibitor sequences (Phe or Leu) to allow identification ofchymotrypsin or chymotrypsin C.
For More Information
Web: bio-rad.com/apoptosis

Ordering Information
Catalog #Description

APO006
APO007
APO008
APO009FLISP FAM-Phe-CMK Serine Protease Assay Kit, 25 tests
FLISP FAM-Leu-CMK Serine Protease Assay Kit, 25 tests
FLISP FAM-Leu-DAP Serine Protease Assay Kit, 25 tests
FLISP FAM-Phe-DAP Serine Protease Assay Kit, 25 tests

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $78.00

Flow Cytometry | 73

Regulated Cell Death Reagents
Apoptosis Reagentsbio-rad.com/apoptosis

Apoptosis Antibodies
Visit bio-rad.com/apoptosis for a full list of our apoptosis products.

Ordering Information
Catalog #Description

Clone

Species Reactivity Applications

Host

Formats

AHP963
AHP1423
AHP2717
AHP964
AHP965
AHP1788
AHP966
AHP967
AHP492
VPA00788
AHP626
AHP1175
AHP2286
VPA00775
MCA1550

Caspase-1
Caspase-2
Caspase-3
Caspase-4
Caspase-5
Caspase-7
Caspase-7
Caspase-8
Caspase-9
Caspase-10 PrecisionAb
Caspase-12
Caspase-14
Caspase-3 Active
Bcl-2 PrecisionAb
Bcl-2

Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
Polyclonal
100

Human
Human, mouse
Human, mouse, rat
Human, mouse
Human
Human, mouse, rat
Human, mouse, rat
Human, mouse, rat
Human, mouse, rat
Human, mouse
Human, mouse, rat
Human, mouse, rat
Human, mouse, rat
Human
Human

RabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbitRabbit
Mouse

Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.
Pur.

MCA1539
MCA2409

CD95
CD178

LOB 3/17
14C2

Human
Human

Mouse
Mouse

MCA2896
MCA2408GA
MCA2118
MCA2119B
MCA2120

CD178
CD178
Granzyme B
Granzyme B
Granzyme B

MFL4
10F2
GB7
GB10
GB11

Mouse, rat
Human
Human
Human
Human

Pur., FITC
Pur., A488,
A647, RPE
A488, A647
Pur., EL
Pur.
Biot.
Pur.

IHC-P,* WB
IHC-P,* WB
IHC-P, WB
IHC-P,* WB
WB
IHC-P,* WB
IHC-P,* WB
WB
WB
WB
IHC-P,* WB
WB
IHC-P, WB
WB
FC,* IHC-F,
IHC-P,* IP, WB
FC, IP
E, FC, IP
FC, FNE, FC, FN, IP
IHC-P,* WB
E
E, FC,* IHC-F,
IF/ICC , IP

Hamster
Mouse
Mouse
Mouse
Mouse

Price,
U.S. $
305.00305.00305.00305.00305.00305.00Applications: E, ELISA; FC, flow cytometry; FN, functional assays; IF/ICC, immunofluorescence/immunocytochemistry;
IHC-F, immunohistology-frozen; IHC-P, immunohistology-paraffin; IP, immunoprecipitation; WB, western blotting.
Formats: Axxx, Alexa Fluor; Biot., biotin; EL, low endotoxin; FITC, fluorescein isothiocyanate; Pur., purified; RPE, R-phycoerythrin.
* Requires special treatment. Refer to product datasheet.

74 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Regulated Cell Death Reagents
Autophagy Reagentsbio-rad.com/autophagy

Autophagy Reagents
Autophagy is the orderly degradation and recycling of cytoplasmic components via the lysosome. It is thought to be a
repair and survival response during stress and infection and has been linked closely to apoptosis. Autophagy can bestudied using antibodies or analysis of active cathepsins. During autophagy, cathepsins hydrolyze and degrade cytoplasmic
proteins, bulk cytoplasm, and dysfunctional organelles for recycling. Increased levels of cathepsin activity have been linked
with conditions such as cancer, arthritis, and Alzheimer’s disease, indicating that cathepsin proteins may be a potential target
for novel therapies.

Cathepsin Detection Kits

The following products are available on
bio-rad-antibodies.com.
Cathepsin detection kits, available in green or red
fluorescence, allow analysis of active cathepsins inwhole, live cells. They use a substrate-based assay with arhodamine 110 or a cresyl violet fluorophore probe. Bothprobes are nonfluorescent when bound through amide
links to two target peptides. Active cathepsins can cleave
the target peptide at an amide link, causing the rhodamine
or mono cresyl violet fluorophores to emit fluorescence.
Rhodamine 110 emits at 525 nm when excited at 500 nm
and can be detected using microscopy or flow cytometry
using the 488 nm laser and a 525/35 nm filter. Cresyl violetemits at 610 nm when excited at 540–590 nm.
Uncleaved target peptides have excitation and emission
wavelengths of 592 nm and 628 nm respectively, resulting
in minimal cellular autofluorescence.
The photostable substrates for cathepsin B: (z-argininearginine)2, cathepsin K: (z-leucine-arginine)2 and cathepsin

L: (z-phenylalanine-arginine)2 easily cross the plasma
membrane and intracellular organelle membranes oflive cells. Cathepsin activity can be determined by theaccumulation of fluorescence when hydrolysis occurs
within organelles. An increase in cathepsin activity results
in a greater fluorescent signal. Cellular analysis may be
performed with a flow cytometer, fluorescence microscope,or plate reader depending on the kit.
All the kits contain a Hoechst stain for labeling nuclei, andthe Magic Red kits contain acridine orange for lysosome
and organelle identification.
The cathepsin kits are suitable for cells in suspension,
adherent cells, and thin frozen tissue sections (but not fixedor paraffin-embedded cells) from many species including
mammalian, insect, and yeast. Various cell types, including
Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7, andU937 cells, have also been successfully studied withthese kits.
For More Information
Web: bio-rad.com/autophagy

Ordering Information
Catalog #Description

ICT937
ICT938
ICT939
ICT941
ICT942
ICT9151
ICT9152

Magic Red Cathepsin B Kit, 25 tests
Magic Red Cathepsin B Kit, 100 tests
Magic Red Cathepsin K Kit, 25 tests
Magic Red Cathepsin L Kit, 25 tests
Magic Red Cathepsin L Kit, 100 tests
Green Cathepsin B Kit, 25 tests
Green Cathepsin B Kit, 100 tests

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
78.00

78.00

Flow Cytometry | 75

Regulated Cell Death Reagents
Autophagy Reagentsbio-rad.com/autophagy

Flow Cytometry Autophagy Assay
Autophagy probe red can be quickly and easily detectedby flow cytometry in cells undergoing autophagy, with
optimal excitation at 590 nm and peak emission at
620 nm (ZE5 cell analyzer settings: 561 nm laser and615/24 or 640/20 filter).

The following products are available on
bio-rad-antibodies.com.
Autophagy probe red is a cell permeant, aliphatic moleculethat fluoresces brightly when inserted into the lipid
membranes of autophagosomes and autolysosomes.
Autophagy is a dynamic process typically divided into
three stages.

For More Information
Web: bio-rad.com/autophagy

Ordering Information
Catalog #Description

APO010A
APO010B

Autophagy Assay Red Detection Kit, 50 tests
Autophagy Assay Red Detection Kit, 200 tests

Price, U.S. $78.00

Autophagy Antibodies
Visit bio-rad.com/autophagy for a full list of our autophagy products.

Ordering Information
Catalog #Description

Clone

Species Reactivity Applications

Host

Formats

Price, U.S. $AHP1009
AHP2167

Polyclonal
Polyclonal

Human, mouse, rat
HumanFC, IHC-P,* WB
IF/ICC, WB

RabbitRabbitPur.
Pur.

305.00305.00

AHP2448
MCA4707T
MCA2315GA

Beclin-1
MAP1LC3A/B(N-Terminal)
CD107a (LAMP-1)
CD107a (LAMP-1)
CD107a (LAMP-1)

Polyclonal
1D4B
4E9/11

Mouse, rat
Mouse
Pig

RabbitRat
Mouse

CD107b (LAMP-2)
CD107b (LAMP-2)

Polyclonal
M3/84

Human, mouse, rat
Mouse

Pur.
Pur.
Pur., A647,
FITC
Pur.
Pur.

305.00305.00305.00

AHP1634
MCA2293
MCA2558GA

CD107b (LAMP-2)

AC17Dog, human

IF/ICC, IHC-P, WB
FC, IHC-F, IF/ICC, IP
FC,* IHC-F, IF/ICC,
IHC-P,* IP, WB
IHC-F, WB
FC,* IHC-F, IHC-P,
IP, WB
FC,* IF/ICC, IP, WB

Rabbit
Mouse
Mouse

Pur., A647,
FITC, RPE

Applications: E, ELISA; FC, flow cytometry; IF/ICC, immunofluorescence/immunocytochemistry;
IHC-F, immunohistology-frozen; IHC-P, immunohistology-paraffin; IP, immunoprecipitation; WB, western blotting.
Formats: Axxx, Alexa Fluor; FITC, fluorescein isothiocyanate; Pres. Fr., preservative free; Pur., purified; RPE, R-phycoerythrin.
* Requires special treatment. Refer to product datasheet.

76 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Regulated Cell Death Reagents
Pyroptosis Reagentsbio-rad.com/pyroptosis

Pyroptosis Reagents
Pyroptosis is a form of lytic-regulated cell death triggered by perturbations of extracellular and intracellular homeostasis
in the innate immune system. Its proinflammatory nature distinguishes it from other forms of RCD, such as apoptosis andnecroptosis, and it is in fact the most immunogenic of all the cell death mechanisms. It has distinct morphological features
such as cellular swelling, chromatin condensation, and plasma membrane permeabilization. Caspase-1, activated by various
inflammasomes, cleaves gasdermin D into a 199 amino acid carboxy-terminal fragment and a 242 amino acid amino-terminal
fragment. The amino-terminal of cleaved gasdermin D integrates into the cell membrane to form a pore that directly leads tocell death.

Pyroptosis Detection Kits

The following products are available on
bio-rad-antibodies.com.
Pyroptosis/Caspase-1 assay kits utilize our popular
FLICA technology to detect caspase-1 activation. FLICA
probes are cell permeant noncytotoxic fluorescent labeledinhibitors of caspases that covalently bind with active
caspase enzymes. These kits contain the caspase-1
inhibitor reagent YVAD-FMK, which has the preferredbinding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD).
This preferred caspase-1 binding sequence is labeledwith either FAM, a green fluorescent dye, or 660, a far
red fluorescent dye, and linked to a fluoromethyl ketone(FMK) reactive entity. Caspase-1 will not cleave the FLICA
inhibitor probe; instead, it forms an irreversible covalentbond with the FMK group on the reagent and becomes
inhibited from further enzymatic activity. FLICA can be

added directly to the cell culture medium, incubated, andwashed. It is cell permeant and will efficiently diffuse in
and out of all cells. If there is an active caspase-1 enzyme
inside the cell, it will covalently bind with YVAD-FMK andretain the fluorescent signal within the cell. Unbound
FLICA will diffuse out of the cell during the subsequent
wash steps. Therefore, positive cells will retain a higher
concentration of FLICA and fluoresce brighter thannegative cells. There is no interference from procaspases
or inactive forms of the enzymes. After labeling with
FLICA, cells can be counterstained with other reagentsand fixed or frozen. Cells labeled with YVAD-FMK can becounterstained with reagents such as the red live/deadstains propidium iodide and 7-AAD. Nuclear morphology
may be concurrently observed using Hoechst 33342(included in the kit), a blue DNA-binding dye. Cells canbe viewed through a fluorescence microscope or flow
cytometer.
For More Information
Web: bio-rad.com/pyroptosis

Ordering Information
Catalog #Description

ICT9145
ICT9146
ICT9158

Pyroptosis FAM Caspase-1 Kit, 25–50 tests
Pyroptosis FAM Caspase-1 Kit, 100–200 tests
Pyroptosis 660 Caspase-1 Kit, 25 tests

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $78.00

Flow Cytometry | 77

Flow Cytometry
Subject Index

Subject Index
355 nm Laser Upgrade Kit for ZE5 Cell Analyzer, 48
7 Detectors off 355 nm Laser Upgrade Kit, 48
Accessory Reagents, 66
Annexin V Kits, 70
Anti-BrdU Antibodies, 60
Apoptosis Antibodies, 74
Autophagy Antibodies, 76
Calibration Reagents, 65
Caspase Kits, 71
Cathepsin Detection Kits, 75
Cell Proliferation Detection Antibodies, 59
Cell Proliferation Assay Reagents, 56
CFDA-SE Cell Proliferation Assay, 58
CytoTrack Cell Proliferation Assay, 57
Everest Software Developer Kit (ZE5 Automation), 49
Flow Cytometry Autophagy Assay, 76
Kits and Reagents for Cell Analysis, 51
Large Capacity Fluidics Options, 50
LYNX Conjugation Kits, 54
Mitochondrial Membrane Potential Kits, 72
ProFlow Sort Grade 8x Sheath Fluid, 43
ProFlow Sort Grade Water, 44
ProLine Rainbow Beads, 42
ProLine Universal Calibration Beads, 42
PureBlu DAPI Nuclear Staining Dye, 68
PureBlu Hoechst 33342 Nuclear Staining Dye, 68
Pyroptosis Detection Kits, 77
ReadiDrop Cell Viability Assays, 62
ReadiLink Antibody Labeling Kits, 52
Real-Time Apoptosis Fluorescent Microscopy Kit, 72
S3e Cell Sorter Consumables, 40
S3e Cell Sorter IQ/OQ Services, 41
S3e Fluidic Container, 44
Serine Protease Detection Kits, 73
Small Particle Detector (SPD) Upgrade Kit, 49
Standards and Stabilizers, 64
VivaFix Cell Viability Assay, 61
ZE5 Cell Analyzer, 45
ZE5 Cell Analyzer IQ/OQ Services, 47

78 | Flow Cytometry

In the U.S., call toll free at 1-800-424-6723

Cell Imaging
Cell Imaging

80

Cell Imaging
bio-rad.com/ZOE

Cell Imaging
Bio-Rad’s suite of tools for fluorescence microscopy and cell imaging includes the ZOE fluorescent cell imager
and nuclear dyes.

See Also
PureBlu Hoechst
33342 nuclear
staining dye:
page 68.
PureBlu DAPI
nuclear staining dye:
page 68.
ReadiLink antibody
labeling kits:
page 52.
VivaFix cell
viability assays:
page 61.
Cell proliferation
assays: page 56.

Video
Product
Demonstration

bio-rad.com/
ZOEsimplified

ZOE Fluorescent Cell Imager
The ZOE fluorescent cell imager is a complete imaging
system that is suitable for routine cell culture and imaging
applications. It eliminates the complexities of cell imaging
by combining the ease of use of a personal tablet with thepower of an inverted microscope. An intuitive Androidbased touch-screen interface is used to control brightfield,
three fluorescence channels, and the integrated
digital camera.
As a fully integrated system with long-life light-emitting
diodes (LEDs), the ZOE cell imager is a robust imaging
device ready for intensive daily use. There are no timeconsuming setup and hardware adjustments to perform(optical and illumination alignment, changing filter sets orobjective lenses) or parts with a limited life span that could
result in costly maintenance (replacing mercury arc lamps).
The ZOE cell imager uses high-quality hard-coated filter
sets that provide high light transmission and long life.
Its 20x plan achromatic objective lens is mounted in a
proprietary manner, resulting in a wide field of view
(0.70 mm2) that is ~180% greater than that of a traditionallymounted 20x objective lens. When zoomed out it isapproximately equivalent to that of a 4x objective.
When needed, use the pinch-to-zoom gesture to magnify
up to 20x by digital zoom while retaining resolution (1 µm).

ZOE Fluorescent Cell Imager■■

Product features include:■ implified cell imaging — the intuitive touch-screenSinterface allows users to view cells, capture images,
and create multichannel merges

■

■
■

■

Flexible system — brightfield and three fluorescencechannels enable use for routine cell culture applications
as well as more sophisticated imaging applications
 luorescence at your bench — an integrated lightF
shield permits fluorescence imaging in ambient light obust construction — fully integrated system withR
long-life LEDs is ready for intensive daily use
 ED light sources — thousands of hours of illumination
Lthat are instantly ready after power on arge field of view — the motorized stage and large
Lfield of view allow you to see more of your sample, fasterSmall footprint — compact size accommodates
crowded laboratory benches

For More Information
Web: bio-rad.com/ZOE
Request or download bulletin: 6585

Ordering Information
Catalog #Description

1450031ZOE Fluorescent Cell Imager, 120–240 V, includes instrument, power supply, USB flash drive

80 | Cell Imaging

In the U.S., call toll free at 1-800-424-6723

Cell Imaging
bio-rad.com/ZOE

Kits and Reagents for Cell Imaging
Bio-Rad offers a variety of kits and reagents for use with the ZOE fluorescent cell imager.

Ordering Information
Catalog #Description

Nuclear Staining Dyes
1351303
PureBlu DAPI Nuclear Staining Dye, pkg of 1, includes 5 x 50 µg vials of DAPI powder
1351304
PureBlu Hoechst 33342 Nuclear Staining Dye, pkg of 1, includes 5 x 56 µg vials of Hoechst 33342 powder
Antibody Labeling Kits
1351002
ReadiLink 492/516 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer,and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351003
ReadiLink 555/570 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer,and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351004
ReadiLink 594/610 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer,and dyed quench buffer for labeling 2 x 50 µg of antibodies
Fixable Viability Dyes
1351111
VivaFix 353/442 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO,
for running 200 assays
1351115
VivaFix 498/521 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO,
for running 200 assays
1351116
VivaFix 547/573 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO,
for running 200 assays
1351117
VivaFix 583/603 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO,
for running 200 assays

In the U.S., call toll free at 1-800-424-6723

Cell Imaging | 81

Cell Imaging
Subject Index

Subject Index
Kits and Reagents for Cell Imaging, 81
ZOE Fluorescent Cell Imager, 80

82 | Cell Imaging

In the U.S., call toll free at 1-800-424-6723

Chromatography:
Laboratory andProcess Separations
Chromatography Overview

84

Chromatography MediaIon Exchange Chromatography
Analytical Grade Ion Exchange Chromatography
Mixed-Mode Chromatography
Affinity ChromatographySize Exclusion Chromatography
Hydrophobic Interaction Chromatography
Screening and Application Packs, and Standards

85
88
94
99
106
117
119
121

Chromatography Columns
Prepacked Chromatography Columns
Empty Columns

124
124
140

Medium-Pressure Chromatography Systems
Fraction Collectors

150
162

Chromatography Accessories

166

Process-Scale Separations

171

Chromatography Overview
bio-rad.com/chromatography

Chromatography Overview
A Range of Options
Bio-Rad offers a wide selection of chromatography tools for the life scientist involved in analytical, preparative, or process
chromatography. We have become known as a quality provider of chromatography resins for reagent cleanup andbiomolecule purification, manufacturing flexible and intuitive instrumentation and software and prepacked and empty columns
for sample separations, screening and method development, and biopharmaceutical manufacturing applications.
Throughput, capacity, selectivity, resolution, and process economics are among the considerations when selecting any
chromatography resin, column, or instrument. We offer products for each phase of purification and manufacture resins forany scale — for purification of nanograms to kilograms of the target molecule.
Bio-Rad offers a wide range of lab and process chromatography resins for ion exchange, mixed mode, affinity, size exclusion, andhydrophobic interaction chromatography as well as chromatography standards. For convenient sample preparation products, seepage 1. Bio-Rad’s process chromatography resins are used worldwide to manufacture biotherapeutics and diagnostic products.
Development, Application, and Validation Support
Bio-Rad provides development and application support for large-scale separations. We also offer regulatory support files to help
validate the chromatography resins used in your processes. These files contain information essential to validation, including
general product information, specification test procedures, identification tests, and biological safety data.
Small Molecules, MW <3,000

What Molecules
Can Be Purified?

Nucleic Acids, Proteins, Peptides, MW >3,000

Ion
Exchange

Size
Exclusion

Hydrophobic
Interaction

AG Resins, pg. 94

Bio-Beads S-X Resins,
pg. 119

Bio-Beads SM-2 Resin,pg. 120

Mixed-ModeAffinitySize
Exclusion

Hydrophobic
Interaction

Nuvia aPrime 4A Resin,
pg. 99xx

Profinity Resins,
pg. 108

Bio-Gel P Resins,
pg. 117

Macro-Prep Methyl
Resin, pg. 119

Nuvia cPrime Resin,
pg. 99Affi-Gel and
Affi-Prep Resins,
pg. 112

Bio-Gel A Resins,
pg. 118

Macro-Prep t-Butyl
Resin, pg. 119

Bio-Beads S-X Resins,
pg. 119

Bio-Beads SM-2,
pg. 120

ENrich SEC Columns,
pg. 126

EconoFit Columns,
pg. 127

Aminex Columns, pg. 137
Chelex 100 Resins, pg. 94
Bio-Rex Resins, pg. 94
Ion
Exchange
Nuvia Resins, pg. 88
UNOsphere Resins,
pg. 88Macro-Prep Resins,
pg. 91CHT XT Media, pg. 101
xx.

UNO Columns, pg. 136

CHT Type I andType II Media, pg. 101

Bio-Rex 70 Resins,
pg. 94

MPC Type I Media, pg. 104

ENrich IEX Columns,
pg. 125

CFT Type II Media, pg. 104

Foresight Columns,
Plates, pg. 130
117, 118
EconoFit Columns,
pg. 127

Bio-Gel HT Media,
pg.105
Bio-Gel HTP Media,
pg. 105

Nuvia IMAC Resin,pg. 106
Nuvia IMAC
Uncharged Resin,pg. 106
101
UNOsphere SUPrA,
pg. 111

EconoFit Columns,
pg. 127

Foresight Columns,
Plates, pg. 130
117, 118
EconoFit Columns,
pg. 127

Foresight Columns, Plates,
pg. 130
117, 118
Foresight Pro Columns
pgs. 132,
117, 175
118
EconoFit Columns,
pg. 127
87
Bio-Scale CHT Type I Columns,
pg. 136

84 | Chromatography: Laboratory and Process Separations

Learn More about the Technology
Web: bio-rad.com/tech/chrom

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
bio-rad.com/chromresin

Chromatography Media
Bio-Rad offers process-scale developers a selection of media for protein separation using ion exchange, mixed mode,hydroxyapatite and fluoroapatite, affinity, size exclusion (gel filtration), and hydrophobic interaction chromatography. These
media offer high binding capacity, base stability, and high flow rates and are available in multi-liter quantities.
For More Information
Request or download bulletin: 6713
Chromatography Media Selection Guide
Suitability**
Packing
Format*Analytical
Scale

Pilot/
Preparative Process
Scale
Scale

AG 1

B, GC

++++

++++

AG MP-1M

B

++++

++

Bio-Rex 5 andAG 4-X4

B

++++

++

UNO Q

MC

++++

Macro-Prep High Q
and Macro-Prep
High Q 3HT

B, E

+++

++++

Macro-Prep 25 Q

B

++++

Macro-Prep DEAE

B, E

UNOsphere Q

Application

Page

++++

Strong exchanger. Separation of low MW peptides,
nucleotides, inorganic ions using different cross-linkages;
high selectivity for anions such as chloride; gravity or lowpressure use

94+

Strong exchanger. Macroporous, equivalent to AG 1 for MW>1,000,000; gravity or low-pressure use

94

Weak exchanger. Used to remove organic acids from sugars;
adsorption of mineral acids; gravity or low-pressure use

94

Strong exchanger. High-resolution biomolecule separation athigh flow rates; pH stability 2–12

136

+++

Strong exchanger. High-capacity biomolecule separation;unique surface chemistry allows contaminant removal;
pH stability 1–10

91

++++

+

Strong exchanger. Similar to Macro-Prep High Q but 25 µm
particle size allows higher-resolution separation; uniquesurface chemistry allows contaminant removal; pH stability
1–10

91

+++

++++

+++

Weak exchanger. High-capacity biomolecule separation;unique surface chemistry allows contaminant removal;
pH stability 1–10

91

B, E, F

++++

++++

++++

Strong exchanger. High-productivity, high-capacity
biomolecule separation; pH stability 1–14

88

AminexHPLC++++

High-pressure separation of carbohydrates, sugars, and smallorganic molecules; delivers industry-standard performance(U.S. Pharmacopeia)

137

Nuvia Q

B, E, F

++++

++++

++++

Strong exchanger. Similar to UNOsphere Q but surface
modification allows extremely high capacity biomolecule
separation; pH stability 1–14

88

Nuvia HP-Q

B, E, F

++++

++++

++++

Strong anion exchange resin specifically designed for the highthroughput purification of large biomolecules

88

AG 50W

B, GC

++++

++++

++++

Strong exchanger. Lower cross-linkages useful for peptide
and nucleotide separation; higher cross-linkages useful forsmall peptide and metals separation and removal of cations;gravity or low-pressure use

94

AG MP-50

B

++++

++

+

Strong exchanger. Macroporous equivalent to AG 50W for
MW >1,000,000; gravity or low-pressure use

94

Bio-Rex 70

B

++++

++

++

Weak exchanger. High capacity for high MW (>1,000,000)
solutes; can be used for purification and fractionation of
peptides, proteins, enzymes, and other cationic molecules.
Amenable to large-scale purification

94

Media
Anion Exchange

Cation Exchange* B, bottle; C, Bio-Scale Mini cartridge (10 or 50 ml); E, EconoFit columns; F, Foresight prepacked plates and columns; GC, gravity
column; HPLC, high-pressure column; MC, medium-pressure column; P, Foresight Pro Columns, (process scale); SC, spin column.
** +, low suitability; ++, moderate suitability; +++, suitable; ++++, high suitability.

In the U.S., call toll free at 1-800-424-6723

continues

Chromatography: Laboratory and Process Separations | 85

Chromatography Media
bio-rad.com/chromresin

Chromatography Media Selection Guide
Suitability**

Media

Packing
Format*Analytical
Scale

Pilot/
Preparative Process
Scale
Scale

Chelex 100

B

++++

++++

UNO S

MC

++++

Macro-Prep 25 S

B

++++

++++

Macro-Prep High S

B, E

+++

Macro-Prep CM

B, E

UNOsphere S

Application

Page

Weak exchanger. Chelating resin removes metals andis suitable for PCR applications; can also be used for
ultrapurification of buffers and ionic reagents; gravity or
low-pressure use. Available in molecular biology andbiotechnology grades

94

Strong exchanger. High-resolution biomolecule separation
at high flow rates; pH stability 2–12

136+

Strong exchanger. Similar to Macro-Prep High S, but
25 µm particle size allows higher-resolution separation;unique surface chemistry allows contaminant removal;
pH stability 1–10

91

++++

+++

Strong exchanger. High-capacity biomolecule separation;unique surface chemistry allows contaminant removal;
pH stability 1–10

91

+++

++++

+++

Weak exchanger. High-capacity biomolecule separation;unique surface chemistry allows contaminant removal;
pH stability 1–10

91

B, E, F

++++

++++

++++

 trong exchanger. High-capacity biomolecule separation;S
pH stability 1–14

88

Nuvia S

B, E, F

++++

++++

++++

Strong exchanger. Similar to UNOsphere S but surface
modification allows extremely high capacity biomolecule
separation; pH stability 1–14

88

Nuvia HR-S

B, E, F

++++

++++

++++

Strong exchanger. Has smaller particle size for high resolution

88

++++

Specialty Ion Exchange
AG 11 A8

B

++++

++

++

Ion retardation — contains cation and anion exchange sitesthat weakly interact with mobile ions; can be used fordesalting of nonionic molecules with water elution, such as
removal of SDS from protein and adsorption of mineral acids

94

AG 501-X8

B

++++

+++

+++

Mixed bed, consists of equivalent amounts of AG 1-X8
and AG 50W-X8. May be used to deionize impure water,urea, formamide, and acrylamide to provide extremely
pure reagents

94

Bio-Rex MSZ 501

B

++++

++

++

Mixed bed, consists of equivalent amounts of Bio-Rex
MSZ 1 and Bio-Rex MSZ 50 resin. Monosized ion
exchange; desalting of water and nonelectrolytes. Ideal forlarge-scale industrial applications

94

Separation of molecules by size; desalting and buffer
exchange; several particle size ranges available with MW
exclusion limits ranging from 100–100,000 D; pH stability 2–10

117

 ractionation of low MW organic polymers and otherFhydrophobic substances in nonpolar solvents from
400–14,000 D

119

Size Exclusion (Gel Filtration
Bio-Gel P

B, C, E, SC

++++

++++

Bio-Beads S-X

B

++++

++++

UNOsphere SUPrA

B, E

++++

+++

Antibody purification; Fc-fusion purification fromlarge volumes of feed/cell culture; development andcommercial-scale mAb purification process applications

111

Affi-Gel Protein A

B, GC

++++

++++

IgG purification from ascites, serum, and culture fluid;
low-pressure resin

112

Affi-Prep Protein A

B, E

++++

++++

IgG purification from ascites, serum, and culture fluid;pressure-stable resin

112

Affi-Gel Blue

B, E, SC

++++

++++

 lbumin removal and enzyme purification; Cibacron BlueA
F3GA dye covalently attached; purification of proteins
with dinucleotide fold

113

++

Affinity++

continues

86 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
bio-rad.com/chromresin

Chromatography Media Selection Guide
Suitability**

Media

Packing
Format*Analytical
Scale

Pilot/
Preparative Process
Scale
Scale

Application

Page

DEAE Affi-Gel Blue

B, E

++++

++++

Albumin and protease removal for IgG purification; CibacronBlue F3GA dye covalently attached to DEAE Bio-Gel A

113

CM Affi-Gel Blue

B

++++

++++

 lbumin and protease removal for IgG purification; CibacronA
Blue F3GA dye covalently attached to CM Bio-Gel A

114

Affi-Gel heparin

B

++++

++++

 urification of coagulation factors, plasma proteins, andP
enzymes including nucleases, lipases, and proteases;
binding specific to a variety of enzymes and other proteins

115

Nuvia IMAC

B, E, F

++

++++

++++

Purification of recombinant histidine-tagged proteins;can be charged with other transition metals

106

Nuvia IMAC,
uncharged

B, E

++

++++

++

Purification of recombinant histidine-tagged proteins;can be charged with other transition metals

106

Profinity IMAC

B, E

++++

++++

++

Histidine-tagged protein purification

108

Profinity GST

E

++++

++++

++

GST-tagged protein purification

109

Profinity eXact

B, E, SC

++++

++++

++

One-step affinity tag purification and on-column cleavage

110

Affi-Prep polymyxin B

++++

++++

++++

Removal of endotoxins; pressure-stable resin capableof sanitization procedures with NaOH

115

Affi-Gel boronate

B

++++

++

 ffinity for low MW molecules containing cis hydroxylA(cis-diol) groups; separation of AMP from cyclic AMP

114

Profinity epoxide

B

++++

++++

 ffinity coupling; coupling of nucleophiles such as hydroxyA(–OH), amino (–NH2), or thiol (–SH) groups; based on
UNOsphere base matrix for superb pressure flow
characteristics

109

Affi-Gel 10

B

++++

++++

Affinity coupling; immobilization of ligands with –NH2 groups,
coupling of proteins with pl 6.5–11; low-pressure resin

116

Affi-Gel 15

B

++++

++++

 ffinity coupling; immobilization of ligands withA
–NH2 groups, coupling of proteins with pl <6.5;
low-pressure resin

116

Affi-Gel Hz

B

++++

++++

Affinity coupling; immobilization of IgG molecules via
their Fc region116

Affi-Gel 102

B

++++

++++

Affinity coupling of ligands with –COOH groups via EDAC
coupling chemistry

116

++++

Mixed-Mode
Nuvia aPrime 4A

B, E, F

++++

++++

++++

Biomolecule purification, a hydrophobic anion exchange resin

99

Nuvia cPrime

B, E, F

++++

++++

++++

Biomolecule purification, a hydrophobic cation exchange resin

99

CHT XT

B, E, F, P

++++

++++

++++

Antibody purification; virus purification/removal; DNApurification/removal; aggregate and endotoxin removal

101

CHT Type I

B, MC, E,
F, P

++++

++++

++++

Antibody purification (high capacity); virus purification/
removal; DNA purification/removal; aggregate andendotoxin removal

101

CHT Type II

B, E, F, P

++++

++++

++++

 ntibody purification; removal of albumin from feedstream;
A
vaccine/VLP purification

101

MPC Type I

B, E, F

++++

++++

++++

Antibody purification; virus purification/removal; DNApurification/removal; aggregate and endotoxin removal

104

CFT Type II

B, E

++++

++++

++++

Similar properties to CHT but exhibits greater stability in thelower pH range (5.6)

104

Bio-Gel HT

B

++++

+++

 urification of proteins, nucleic acids, and otherPbiomolecules; crystalline hydroxyapatite

105

Bio-Gel HTP

B

++++

+++

Similar to Bio-Gel HT but in powder form

105

DNA grade
Bio-Gel HTP

B

++++

+++

Similar to Bio-Gel HTP with smaller particle size; selectivity
for double-stranded DNA; single- and double-stranded
DNA separation

105

continues

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 87

Chromatography MediaIon Exchange Chromatography

bio-rad.com/ionexchange

Chromatography Media Selection Guide
Suitability**

Media

Packing
Format*Analytical
Scale

Pilot/
Preparative Process
Scale
Scale

Application

Page

Hydrophobic Interaction
Macro-Prep Methyl

B, E

++++

++++

++++

Separation of proteins based on relative hydrophobicity;
pH stability 1–10

119

Macro-Prep t-Butyl

B, E

++++

++++

++++

Separation of proteins based on relative hydrophobicity;
pH stability 1–10

119

Bio-Beads SM-2

B

++++

++++

++++

Useful for the adsorption of nonpolar substances or surface
active agents from aqueous solutions120

 , bottle; C, Bio-Scale Mini cartridge (10 or 50 ml); E, EconoFit columns; F, Foresight prepacked plates and columns; GC, gravity column; HPLC, high*B
pressure column; MC, medium-pressure column; SC, spin column. ** +, low suitability; ++, moderate suitability; +++, suitable; ++++, high suitability.

Ion Exchange Chromatography
See Also
Chromatographysystems:
page 150.
Prepacked columns:
page 124.
Ion exchange
chromatography
standards:
page 122.
Sample preparation
products: page 1.
Bio-Scale Mini
cartridges:
page 117.
EconoFit columns
and packs:
page 127

UNOsphere and Nuvia Ion Exchange Media
Bio-Rad’s ion exchange resins are scalable and robust. They
are designed to meet the needs of the biopharmaceuticalindustry for capture, intermediate, and polishing stages ofpurification. UNOsphere and Nuvia resins are bioprocesscompatible and may also be used at laboratory scales forhigh-performance applications. Benefits include:■Efficient capture from crude feedstreams■

Optimization to operate under 2 bar at 1,200 cm/hr■■

 arge-pore design results in ultra-high binding capacitiesLat fast linear velocities
Fully supported for regulatory information

UNOsphere Q and S Resins
UNOsphere Q and S resins are strong anion and cationexchange resins, respectively, and may be used at any
stage of the purification process.
For More Information
Web: bio-rad.com/unosphere
Download bulletins: UNOsphere Q resin — 2724 and 2729;
UNOsphere S resin — 2669 and 2678

Nuvia Q, S, and HR-S Resins
Nuvia ion exchange resins are a family of next-generation
ion exchange products built on an industry-provenproprietary base matrix technology. Nuvia resins provide
very high capture and exceptional flow properties designed
to meet current and future process needs. Nuvia Q and S
are flexible alternatives that may be used as capture
and/or polishing solutions. Nuvia HR-S is a high-resolution
cation exchanger designed for intermediate and final
polish applications.

88 | Chromatography: Laboratory and Process Separations

■

■

■

■

Use less resin to purify a given amount of product educe cycle time and increase productivity by operatingR
at higher flow rates
 educe cost and space requirements by decreasingR
buffer consumption
 educe capital and operating expenses by usingR
smaller columns■

Chemical stability for repetitive clean-in-place cycles■

Flexibility for capture or polish steps■

Fully supported for regulatory submission

Nuvia HP-Q Resin
Nuvia HP-Q is a strong anion exchange resin specificallydesigned for the high-throughput purification of large
biomolecules. The large pores, optimized with surface
extenders, together with the rigid base beads provide
fast mass transfer kinetics at high flow rates for large
biomolecule purification.
■

 ptimized surface extender/ligand density allows highO
dynamic binding capacity■

Favorable pressure flow characteristics at scale■

Base stability for excellent process economics

For More Information
Web: bio-rad.com/nuvia
Download bulletin: 5984
Request or download bulletins: 5987, 6128, 6129, 6241, and 6448

In the U.S., call toll free at 1-800-424-6723

Chromatography MediaIon Exchange Chromatography

bio-rad.com/ionexchange

Properties of UNOsphere and Nuvia
Property

UNOsphere Q

UNOsphere S

Nuvia Q

Nuvia S

Nuvia HR-S

Nuvia HP-Q

Type of ion exchanger

Strong anion

Strong cation

Strong anion

Strong cation

Strong cation

Strong anion

Functional group–N+(CH3)3–SO3––N(CH3)3+

–SO3–

–SO3–

–N(CH3)3+

Total ionic capacity

120 µeq/ml

269 ± 50 µeq/ml

100–170 µeq/ml

90–150 µeq/ml

100–180 µeq/ml

48–88 µeq/ml

Median particle size, μm

120

80

85

85

50

50

60 mg IgG/ml—
30 mg IgG/ml—>170 mg/ml——>110 mg/ml——≥70 mg/ml—>50 mg/ml at 100 cm/hr**——

Dynamic binding capacity*,**At 100 cm/hr
180 mg BSA/ml
At 300 cm/hr—
At 600 cm/hr
125 mg BSA/ml
Recommended linear
flow rate range

50–300 cm/hr

50–300 cm/hr

50–600 cm/hr

50–300 cm/hr

50–200 cm/hr

50–300 cm/hr

pH stability(accelerated, 60°C)

1–14

1–14

2–14 short term

2–14 short term

2–14 short term

2–13 short term——

4–12 long term

4–13 long term

4–13 long term

2–10 long term

0.5–1.0 N NaOH

0.5–1.0 N NaOH

0.5–1.0 N NaOH

0.5–1.0 N NaOH

0.5–1.0 N NaOH

0.5 N NaOH

Sanitization* 10% breakthrough capacity determined with a 5.0 mg/ml human IgG and 5.0 mg/ml BSA in a 1.1 x 20 cm column.
** Nuvia HP-Q 10% breakthrough capacity determined with 1.1 mg/ml thyroglobulin in a 0.7 x 5.5 cm column.

Ordering Information
Description

Catalog #Catalog #Nuvia S — Ultra high capacity strong cation exchange process media
Nuvia S Media, 25 ml
Nuvia S Media, 100 ml
Nuvia S Media, 500 ml
Nuvia S Media, 5 L
Nuvia S Media, 10 L

20% EtOH Shipping Solution
15603111560313156-0315
12018126
156-0317

2% BnOH Shipping Solution
12003704
12003705
12003706

Nuvia HR-S — High-resolution strong cation exchange process media
Nuvia HR-S Media, 25 ml
Nuvia HR-S Media, 100 ml
Nuvia HR-S Media, 500 ml
Nuvia HR-S Media, 5 L
Nuvia HR-S Media, 10 L

20% EtOH Shipping Solution
15605111560513156-0515
12018125156-0517

2% BnOH Shipping Solution
12003712
12003713
12003714
12018182
12003715

Nuvia Q — Ultra high capacity strong anion exchange process media
Nuvia Q Media, 25 ml
Nuvia Q Media, 100 ml
Nuvia Q Media, 500 ml
Nuvia Q Media, 5 L
Nuvia Q Media, 10 L

20% EtOH Shipping Solution
15604111560413156-0415
12018183
156-0417

2% BnOH Shipping Solution
12003708
12003709
12003710

Nuvia HP-Q — High-performance strong anion exchange
process media
Nuvia HP-Q Media, 25 ml
Nuvia HP-Q Media, 100 ml
Nuvia HP-Q Media, 500 ml
Nuvia HP-Q Media, 5 L
Nuvia HP-Q Media, 10 L

20% EtOH Shipping Solution

2% BnOH Shipping Solution

12006693
12006691
12006660
12006659
12007023

12007022
12007018
12007019
12007033
12006994

UNOsphere Q — High-capacity strong anion exhange process media
UNOsphere Q Media, 25 ml
UNOsphere Q Media, 100 ml
UNOsphere Q Media, 500 ml
UNOsphere Q Media, 10 L

20% EtOH Shipping Solution
1560101
1560103156-0105156-0107

2% BnOH Shipping Solution
12003696
12003697
12003698
12003699

UNOsphere S — High-capacity strong cation exchange process media
UNOsphere S Media, 25 ml
UNOsphere S Media, 100 ml
UNOsphere S Media, 500 ml
UNOsphere S Media, 10 L
EtOH, ethanol; BnOH, benzyl alcohol

20% EtOH Shipping Solution
15601111560113156-0115
156-0117

2% BnOH Shipping Solution
12003700
12003701
12003702
12003703
continues

In the U.S., call toll free at 1-800-424-6723

12003707

12003711

Chromatography: Laboratory and Process Separations | 89

Chromatography MediaIon Exchange Chromatography

bio-rad.com/ionexchange

Ordering Information
Catalog #Description

U.S. $

Foresight Plates*732-4714F oresight UNOsphere Q, 20 μl
732-4710
F oresight UNOsphere S, 20 μl
732-4703F oresight Nuvia Q, 20 μl
 oresight Nuvia S, 20 μl
732-4701F
732-4707
Foresight Nuvia HR-S, 20 μl
12006908
F oresight Nuvia HP-Q, 20 μl

551.25551.25551.25551.25540.75551.25

Description

Catalog #Catalog #Foresight Columns
Foresight UNOsphere Q
Foresight UNOsphere S
Foresight Nuvia Q
Foresight Nuvia S
Foresight Nuvia HR-S
Foresight Nuvia HP-Q

1 ml
732-4732
732-4730
732-4721
732-4720
732-4723
12007020

Price, U.S. $5 ml
Price, U.S. $104.25732-4752
192.14104.25732-4750
192.14115.83732-4741
213.49115.83732-4740
213.49126.79732-4743
231.53126.79 12007021
231.53

Foresight RoboColumn Units**
Foresight UNOsphere Q
Foresight UNOsphere S
Foresight Nuvia Q
Foresight Nuvia S
Foresight Nuvia HR-S
Foresight Nuvia HP-Q
Catalog #200 μlPrice, U.S. $600 μlPrice, U.S. $732-4819
732-4813
732-4804
732-4801
732-4831
12007013

192.94
192.94212.78212.23203.96126.79732-4820
732-4814
732-4805
732-4802732-4832
12007014

231.53231.53254.68254.68259.09259.09Description

U.S. $

EconoFit Columns
12009307
EconoFit UNOsphere Q Column, 1 x 1 ml , 7 mm x 25 mm
12009302
EconoFit UNOsphere Q Column, 1 x 5 ml, 16 mm x 25 mm
12009301
EconoFit UNOsphere Q Columns, 5 x 1 ml, 7 mm x 25 mm
12009303
EconoFit UNOsphere Q Columns, 5 x 5 ml, 16 mm x 25 mm
12009308
EconoFit UNOsphere S Column, 1 x 1 ml, 7 mm x 25 mm
12009305
EconoFit UNOsphere S Column, 1 x 5 ml, 16 mm x 25 mm
12009304
EconoFit UNOsphere S Columns, 5 x 1 ml, 7 mm x 25 mm
12009306
EconoFit UNOsphere S Columns, 5 x 5 ml, 16 mm x 25 mm
12009290
EconoFit Nuvia Q Column, 1 x 1 ml, 7 mm x 25 mm
12009291
EconoFit Nuvia S Column, 1 x 1 ml, 7 mm x 25 mm
12009284
EconoFit Nuvia HR-S Column, 1 x 1 ml, 7 mm x 25 mm
12009282
EconoFit Nuvia HP-Q Column, 1 x 1 ml, 7 mm x 25 mm
EconoFit AEX and CEX Screening Packs
 conoFit AEX Screening Pack, includes 1 ml column each of Macro-Prep High Q, Macro-Prep High Q-3HT, Macro-Prep 145.00
17007892
E
DEAE, UNOsphere Q, Nuvia Q, and Nuvia HP-Q
17007872E conoFit CEX Screening Pack, includes 1 ml column each of Macro-Prep High S, Macro-Prep CM, UNOsphere S,
97.00
Nuvia S, and Nuvia HR-S
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.

90 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography MediaIon Exchange Chromatography

bio-rad.com/ionexchange

See Also

Macro-Prep Ion Exchange MediaIon exchange
chromatography
standards: page 122.

Macro-Prep ion exchange resins are designed to provide
high resolution and high capacity for preparative
separations. The rigid methacrylate beads exhibit little
shrinkage or swelling, making them suitable for both lowand medium-pressure chromatography. The macroporous
resin allows both small and large molecules to accessexchange sites located throughout the chromatography
bed. The physical structure of the resin permits highflow rates at low backpressure. Depending on the resin,
pH conditions, and samples, the products can display
mixed-mode behavior.
Benefits include:■

High capacity for biomolecules■

High resolution of complex biological mixtures■

Rigid methacrylate polymer matrix that allows high flow
rates at modest pressures

Macro-Prep High Q, High Q-3HT, DEAE, High S,
and CM Resins
For maximum flexibility, the product offerings include
Macro-Prep High Q strong anion exchange resin,
Macro-Prep DEAE weak anion exchange resin,
Macro-Prep High S strong cation exchange resin,and Macro-Prep CM weak cation exchange resin.

Macro-Prep Resins

EconoFit Columns

Macro-Prep 25 Q and S Resins
Macro-Prep 25 Q strong anion exchange resin andMacro-Prep 25 S strong cation exchange resin offer
high-resolution separations at high flow rates withmedium pressures. Both resins possess the same rigid,
macroporous, and hydrophilic properties of Macro-Prep
High Q and High S resins, but in a 25 μm bead that offers
higher resolution.
For More Information
Web: bio-rad.com/macroprepSandQ
Download bulletin: 2292

For More Information
Web: bio-rad.com/macroprep
Download bulletins: Macro-Prep DEAE resin — 1942;
High S and High Q resins — 5643 and 5644

Properties of Macro-Prep
Property
Type of resin
Functional ligand
Ionic capacity
Typical binding capacity
Mean particle size**
Recommended linear
flow rate range
pH stability
Storage conditions

High Q*High Q-3HT*

DEAE*

High S

CM

25 Q*

25 S

Strong anion
Strong anion
Weak anion
Strong cation
Weak cation
Strong anion
Strong cation–N+ (CH3)3–N+ (CH3)3–N+ (C2H5)2
–SO3–
–COO–
–N+(CH3)3–SO3–
400 ± 75 µeq/ml 475 ± 45 µeq/ml 175 ± 75 µeq/ml 160 ± 40 µeq/ml 210 ± 40 µeq/ml 220 ± 70 µeq/ml 110 ± 30 µeq/ml≥25 mg≥37 mg BSA/ml ≥37 mg BSA/ml ≥30 mg BSA/ml ≥49 mg hIgG/ml≥30 mg BSA/ml ≥35 mg hIgG/ml
hemoglobin/ml
50 µm
50 µm
50 µm
50 µm
50 µm
25 µm
25 µm
50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

1–10***
20% ethanol

1–10***
20% ethanol

1–10***
20% ethanol

1–10***
20% ethanol

1–12***
20% ethanol

1–10***
20% ethanol

1–10***
20% ethanol* Do not autoclave in OH– form.
** Mean particle size measured before derivatization.
*** The use of basic reagents greater than pH 10 should be evaluated for each application.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 91

Chromatography MediaIon Exchange Chromatography

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Ordering Information
Description

Catalog #Catalog # acro-Prep High Q — High-capacity strong anion exchange media;
Mvery high flow rates and resolution
Macro-Prep High Q Media, 25 ml
Macro-Prep High Q Media, 100 ml
Macro-Prep High Q Media, 500 ml
Macro-Prep High Q Media, 5 L
Macro-Prep High Q Media, 10 L

20% EtOH Shipping Solution

2% BnOH Shipping Solution

1580040
1560040156-0041
156-0042
156-0043

12003678
12003679
12003680
12003681
12003682

Macro-Prep High Q-3HT — High-capacity strong anion exchange
media; very high flow rates and resolution
Macro-Prep High Q-3HT Media, 25 ml
Macro-Prep High Q-3HT Media, 100 ml
Macro-Prep High Q-3HT Media, 500 ml
Macro-Prep High Q-3HT Media, 5 L
Macro-Prep High Q-3HT Media, 10 L

20% EtOH Shipping Solution

2% BnOH Shipping Solution

Macro-Prep DEAE — High-capacity weak anion exchange media;
very high flow rates and resolution
Macro-Prep DEAE Media, 25 ml
Macro-Prep DEAE Media, 100 ml
Macro-Prep DEAE Media, 500 ml
Macro-Prep DEAE Media, 5 L
Macro-Prep DEAE Media, 10 L

20% EtOH Shipping Solution

2% BnOH Shipping Solution

15800201560020156-0021
156-0022
156-0023

12003673
12003674
12003675
12003676
12003677

Macro-Prep High S — High-capacity strong cation exchange media;
very high flow rates and resolution
Macro-Prep High S Media, 25 ml
Macro-Prep High S Media, 100 ml
Macro-Prep High S Media, 500 ml
Macro-Prep High S Media, 5 L
Macro-Prep High S Media, 10 L

20% EtOH Shipping Solution

2% BnOH Shipping Solution

15800301560030
156-0031
156-0032
156-0033

12003668
12003669
12003670
12003671
12003672

Macro-Prep CM — High-capacity weak cation exchange media; highflow rates and resolution
Macro-Prep CM Media, 25 ml
Macro-Prep CM Media, 100 ml
Macro-Prep CM Media, 500 ml
Macro-Prep CM Media, 10 L
EtOH, ethanol; BnOH, benzyl alcohol. 

20% EtOH Shipping Solution

2% BnOH Shipping Solution

1580070
1560070156-0071
156-0073

12003683
12003684
12003685
12003686

92 | Chromatography: Laboratory and Process Separations

1560040-3HT
1560041-3HT
1560042-3HT
1560043-3HT

continues

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
bio-rad.com/ionexchange

Ion Exchange Chromatography

Ordering Information
Catalog #Description
Macro-Prep 25 Q Media
1530021
Macro-Prep 25 Q Media, 50 ml
153-0023
Macro-Prep 25 Q Media, 1 L
153-0024
Macro-Prep 25 Q Media, 5 L
Macro-Prep 25 S Media
1530031
Macro-Prep 25 S Media, 50 ml
153-0033
Macro-Prep 25 S Media, 1 L
153-0034
Macro-Prep 25 S Media, 5 L
EconoFit Columns
12009273
EconoFit Macro-Prep CM Column, 1 x 1 ml, 7 x 25 mm
12009274
EconoFit Macro-Prep DEAE Column, 1 x 1 ml, 7 x 25 mm
12009265
EconoFit Macro-Prep DEAE Column, 1 x 5 ml, 16 x 25 mm
12009264
EconoFit Macro-Prep DEAE Columns, 5 x 1 ml, 7 x 25 mm
12009266
EconoFit Macro-Prep DEAE Columns, 5 x 5 ml, 16 x 25 mm
12009275
EconoFit Macro-Prep High Q Column, 1 x 1 ml, 7 x 25 mm
12009268
EconoFit Macro-Prep High Q Column, 1 x 5 ml, 16 x 25 mm
12009267
EconoFit Macro-Prep High Q Columns, 5 x 1 ml, 7 x 25 mm
12009269
EconoFit Macro-Prep High Q Columns, 5 x 5 ml, 16 x 25 mm
12009283
EconoFit Macro-Prep High Q-3HT Column, 1 x 1 ml, 7 x 25 mm
12009276
EconoFit Macro-Prep High S Column, 1 x 1 ml, 7 x 25 mm
12009271
EconoFit Macro-Prep High S Column, 1 x 5 ml, 16 x 25 mm
12009270
EconoFit Macro-Prep High S Columns, 5 x 1 ml, 7 x 25 mm
12009272
EconoFit Macro-Prep High S Columns, 5 x 5 ml, 16 x 25 mm
EconoFit AEX and CEX Screening Packs
17007892
EconoFit AEX Screening Pack, includes 1 ml column each of Macro-Prep High Q, Macro-Prep High Q-3HT,
Macro-Prep DEAE, UNOsphere Q, Nuvia Q, and Nuvia HP-Q
17007872
EconoFit CEX Screening Pack, includes 1 ml column each of Macro-Prep High S, Macro-Prep CM,
UNOsphere S, Nuvia S, and Nuvia HR-S

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 93

Chromatography Media
Analytical Grade Ion Exchange Chromatography

bio-rad.com/agresins

Analytical Grade Ion Exchange Chromatography

AG, Bio-Rex, and Chelex Resins
AG (analytical grade) resins — AG resins are primarily
used for the separation of low MW compounds suchas inorganic ions, organic acids, nucleic acids, orcarbohydrates. They are available as both strong andweak cation and anion exchangers and as mixed-bed ion
exchangers. Many are available in several ionic forms andcan be converted from one form to another■■■AG 1-X8 strong anion exchanger resins — useful forsample preparation, enzyme assays, metal separations,and peptide, protein, and nucleic acid separations
 G 50W-X8 strong cation exchanger resins —A
particularly useful for the removal of ethidium bromide
and propidium iodide from DNA samples
AG 501-X8 mixed-bed resins — useful for deionizationof water and nonelectrolyte solutions
Bio-Rex resins — available as weak anion andcation exchangers and as monosized mixed-bed ion
exchangers. Bio-Rex 70 resins are macroreticularresins with a high capacity for high molecular weightcompounds used for the purification and fractionationof proteins and peptides.

Chelex 100 resins — contain paired iminodiacetate ionscoupled to a styrene divinylbenzene support. They are
unique chelating resins that bind polyvalent cations withhigh selectivity and are used to remove metal ions fromsamples and buffers.
For More Information
Web: bio-rad.com/agresins
Request or download bullein: 6904

Analytical Grade Resin Wet Mesh and Equivalent Diameters
Wet mesh (U.S. standard)
Diameter, µm

16
1,180

20
850

40
425

94 | Chromatography: Laboratory and Process Separations

50
300

80
180

100
150

140
106

200
75

270
53

325
45

400
38

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Analytical Grade Ion Exchange Chromatography

bio-rad.com/agresins

See Also

Ordering Information
Wet BeadSize, µm

NominalShipping %Water

Catalog #Description

Ionic FormDry Mesh
Size

AG Resins
140-1231
140-1241140-1251140-1253
140-1331
140-1341
140-1351
140-1421
140-1431
140-1441*
140-1451*
140-1422140-1443
140-1453
140-1444
140-1454
142-1231
142-1241*
142-1251142-1331
142-1341
142-1351*
142-1421
142-1431
142-1441*
142-1451*
142-1641
142-1651
141-1831
141-1841
141-1851
143-0841
140-4341*
1437424*
142-6424*,**,***
142-6425*,**
142-7834*

AG 1-X2 Resin, 500 g
AG 1-X2 Resin, 500 g
AG 1-X2 Resin, 500 g
AG 1-X2 Resin, 500 g
AG 1-X4 Resin, 500 g
AG 1-X4 Resin, 500 g
AG 1-X4 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 1-X8 Resin, 500 g
AG 50W-X2 Resin, 500 g
AG 50W-X2 Resin, 500 g
AG 50W-X2 Resin, 500 g
AG 50W-X4 Resin, 500 g
AG 50W-X4 Resin, 500 g
AG 50W-X4 Resin, 500 g
AG 50W-X8 Resin, 500 g
AG 50W-X8 Resin, 500 g
AG 50W-X8 Resin, 500 g
AG 50W-X8 Resin, 500 g
AG 50W-X12 Resin, 500 g
AG 50W-X12 Resin, 500 g
AG MP-1M Resin, 500 g
AG MP-1M Resin, 500 g
AG MP-1M Resin, 500 g
AG MP-50 Resin, 500 g
AG 4-X4 Resin, 500 g
AG 501-X8 Resin, 100 g
AG 501-X8 Resin, 500 g
AG 501-X8(D) Resin, 500 g
AG 11 A8 Resin, 500 g

Chloride
Chloride
Chloride
AcetateChloride
Chloride
Chloride
Chloride
Chloride
Chloride
ChlorideHydroxide
AcetateAcetateFormateFormateHydrogenHydrogenHydrogenHydrogenHydrogenHydrogenHydrogenHydrogenHydrogenHydrogenHydrogenHydrogen
Chloride
Chloride
ChlorideHydrogenFree base
H+ + OH–
H+ + OH–
H+ + OH–
Self-adsorbed

50–100100–200
200–400
200–400
50–100100–200
200–400
20–50
50–100100–200
200–400
20–50
100–200
200–400
100–200
200–400
50–100100–200
200–400
50–100100–200
200–400
20–50
50–100100–200
200–400
100–200
200–400
50–100100–200
200–400
100–200
100–200
20–50
20–50
20–50
50–100

180–500
106–250
75–18075–180
180–425
106–250
63–150300–1,180
180–425
106–180
45–106300–1,180
106–180
45–106106–180
45–106300–1,180
106–300
75–180
180–425
106–250
63–150300–1,180
180–425
106–250
63–150
106–250
53–106150–300
75–150
38–75
75–15075–150300–1,180
300–1,180
300–1,180
180–42570­–7870­–7870­–7870­–7859­– 6559­– 6559­– 6539­–45
39­–45
39­–45
39­–45
39­–45
39­–45
39­–45
39­–45
39­–4575­–8375­–8375­–83
64–72
64–72
64–72
50–56
50–56
50–56
50–56
42–48
42–48
56–64
56–64
56–64
46–52
—
43–55
43–55
43–55
—

625.00505.00505.00775.00460.00625.00695.00355.00545.00545.00585.00440.00695.00595.00595.00595.001,050.00
730.001,290.00
580.00590.00560.00460.00730.00620.00735.001,295.00
2,060.00
745.00795.001,075.00
865.00545.00545.00360.00510.00560.00Chloride
Chloride
Sodium
Sodium
Sodium
Sodium
H+ + OH–

100–200
200–400
20–50
50–100100–200
200–400
25–35

75–15045–75
300–1,180
150–300
75–15045–75
500–700

50–58
50–58
65–74
65–74
65–74
65–74
—

975.001,510.00
795.00795.00795.00795.00435.00

H+ + OH–

25–35

500–700—

435.00

Bio-Rex Resins
140-7841
Bio-Rex 5 Resin, 500 g
140-7851
Bio-Rex 5 Resin, 500 g
142-5822
Bio-Rex 70 Resin, 500 g
142-5832*
Bio-Rex 70 Resin, 500 g
142-5842
Bio-Rex 70 Resin, 500 g
142-5852*
Bio-Rex 70 Resin, 500 g
142-7425*
Bio-Rex MSZ 501(D)Resin, 500 g
Bio-Rex MSZ 501(D)142-7426*
Resin, 10 kg

Price, U.S. $Poly-Prep ion
exchange columns:
page 142.

continues

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 95

Chromatography Media
Analytical Grade Ion Exchange Chromatography

See Also
Ion exchange
chromatography
standards:
page 122.

bio-rad.com/agresins

Ordering Information

Catalog #Description

Chelex Resins
142-2822
142-2832*
142-2842**
142-2843
1422825
142-2827
142-2826

Chelex 100 Resin, 500 g
Chelex 100 Resin, 500 g
Chelex 100 Resin, 500 g
Chelex 100 Resin, 10 kg
Chelex 100 Resin, 100 g
Chelex 100 Resin, 1 kg
Chelex 100 Resin, 10 kg

Catalog #DescriptionPrice, U.S. $Ionic FormDry Mesh
Size

Wet BeadSize, µm

Sodium
Sodium
Sodium
Sodium
Iron
Iron
Iron

50–100100–200
200–400
200–400
100–200
100–200
100–200

300–1,180
150–300
75–15075–150150–300
150–300
150–300

NominalShipping %Water

Price, U.S. $

68–76
68–76
68–76
68–76

790.00790.00950.00———790.00

AG Resins in Larger Volumes and Special Packaging for Industrial Applications
140-1256140-1255
140-1232
140-1342
140-1352
140-1447
140-1446
140-1425
140-1457
140-1426
140-1432
140-1456
140-1445
140-1424
143-2447
143–2448
143-2450
140-2423
140-2341
142-1232
142-1242
142-1424
142-1423
142-1432
142-1442
142-1452
143-1442
142-1254
141-1842
141-1852
141-1853
142-6427
143-6426**
143-7428*
143-6427***

AG 1-X2 Resin, acetate, 200–400 mesh, 1 kg
AG 1-X2 Resin, acetate, 200–400 mesh, 10 kg
AG 1-X2 Resin, chloride, 50–100 mesh, 10 kg
AG 1-X4 Resin, chloride, 100–200 mesh, 10 kg
AG 1-X4 Resin, chloride, 200–400 mesh, 10 kg
AG 1-X8 Resin, acetate, 20–50 mesh, 10 kg
AG 1-X8 Resin, acetate, 100–200 mesh, 10 kg
AG 1-X8 Resin, chloride, 20–50 mesh, 1 kg
AG 1-X8 Resin, chloride, 100–200 mesh, 5 kg
AG 1-X8 Resin, chloride, 20–50 mesh, 10 kg
AG 1-X8 Resin, chloride, 50–100 mesh, 10 kg
AG 1-X8 Resin, chloride, 200–400 mesh, 10 kg
AG 1-X8 Resin, chloride, 100–200 mesh, 10 kg
AG 1-X8 Resin, hydroxide, 20–50 mesh, 10 kg
AG 1-X8 Resin, hydroxide, 20–50 mesh, biotechnology grade, 10 kg
AG 1-X8 Resin, hydroxide, 100–200 mesh, biotechnology grade, 10 kg
AG 1-X8 Resin, hydroxide, 200-400 mesh, biotechnology grade, 10 kg
AG 2-X8 Resin, chloride, 20–50 mesh, 10 kg
AG 4-X4 Resin, free base, 100–200 mesh, 5 kg
AG 50W-X2 Resin, hydrogen, 50–100 mesh, 10 kg
AG 50W-X2 Resin, hydrogen, 100–200 mesh, 10 kg
AG 50W-X8 Resin, ultrapure, hydrogen, 20–50 mesh, 10 kg
AG 50W-X8 Resin, hydrogen, 20–50 mesh, 10 kg
AG 50W-X8 Resin, hydrogen, 50–100 mesh, 10 kg
AG 50W-X8 Resin, hydrogen, 100–200 mesh, 10 kg
AG 50W-X8 Resin, hydrogen, 200–400 mesh, 10 kg
AG 50W-X8 Resin, sodium, 100–200 mesh, molecular biology grade, 1 kg
AG 50W-X12 Resin, hydrogen, 200–400 mesh, 1 kg
AG MP-1M Resin, chloride, 100–200 mesh, 10 kg
AG MP-1M Resin, chloride, 200–400 mesh, 10 kg
AG MP-1M Resin, nitrate, 200–400 mesh, 10 kg
AG 501-X8 Resin, H+ + OH–, 20–50 mesh, 10 kg
AG 501-X8 Resin, H+ + OH–, 20–50 mesh, 10 kg
AG 501-X8 Resin, H+ + OH–, 20–50 mesh, 10 kg
AG 501-X8(D) Resin, H+ + OH–, 20–50 mesh, 10 kg

790.00790.00790.00790.00790.00790.00790.00790.00790.00790.00950.00950.00950.00790.00790.00790.00790.00950.00950.00790.00790.00790.00790.00790.00950.00950.00950.00950.00continues

96 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Analytical Grade Ion Exchange Chromatography

bio-rad.com/agresins

Ordering Information
Catalog#

Price, U.S. $Description

Particle Size, µm

Ionic Form

Application

Prepacked Poly-Prep Ion Exchange Columns
7316211
Poly-Prep Columns, AG 1-X8
resin, 100–200 mesh, 50

106–180

Chloride

Separation of low molecularweight inorganic anions

245.007316212Poly-Prep Columns, AG 1-X8
resin, 200–400 mesh, 50
Poly-Prep Columns, AG 1-X8
resin, 200–400 mesh, 50

45–106Chloride245.0045–106

Formate7316213

Poly-Prep Columns, AG 50W-X8
resin, 100–200 mesh, 50

106–250

Hydrogen7316214

Poly-Prep Columns, AG 50W-X8
resin, 200–400 mesh, 50

63–150HydrogenFor high-resolution general
purpose separations
Separation of low molecularweight biological compounds
such as carboxylic acids
Separation and concentrationof low molecular weight cations
such as small peptides andamino acids
For high-resolution general
purpose separations

7316221245.00245.00250.00* Also available as biotechnology grade resin.
** Also available as molecular biology grade resin.
*** Molecular biology grade resin; also available in biotechnology grade.

Technical and Reactor Grade Resins
Bio-Rad offers reactor grade Bio-Rex RG 501-X8 resins for power plant deionization systems and large-scale cleanupof metals from waste water.
For More Information
Web: bio-rad.com/agresins

Ordering Information
Catalog #Description

Technical and Reactor Grade Resins
Bio-Rex RG 501-X8 Resin, 1 ft3, for water purification
444-9998*
444-9999
Bio-Rex RG 501-X8 Resin, 500 g

Ionic FormDry Mesh Size

MW Exclusion

H+ + OH –H+ + OH –

20–50
20–50

1,000
1,000

Price, U.S. $Inquire120.00* 1 ft3 corresponds to approximately 20 kg. Larger volumes and special packaging are available on request.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 97

Chromatography Media
Analytical Grade Ion Exchange Chromatography

bio-rad.com/agresins

Molecular Biology and Biotechnology Grade Resins■

Molecular biology grade resins — are chemically

identical to the equivalent analytical grade resins and are
certified to be free of endo- and exonuclease activities
and ligase inhibitors■

B iotechnology grade resins — undergo

special processing and contain fewer than
100 microorganisms/gram
For More Information
Web: bio-rad.com/agresins
Request or download bulletins: 6904, 6905, 6906, 6907, and 6908

Ordering Information
Catalog #Description

Dry MeshIonic Form

Wet BeadSize
Size, µm

Application

Price, U.S. $Molecular Biology Grade Resins
1436424
AG 501-X8 Resin,molecular biology grade, 100 g
1436425
AG 501-X8(D) Resin,molecular biology grade, 100 g
1421253
Chelex 100 Resin,molecular biology grade, 50 g

H+ + OH–

20–50

300–1,180

Deionization

455.00

H+ + OH–

20–50

300–1,180~1,000 MW limit

470.00Sodium200–400

75–150DNA or RNA sample preparation
for downstream detection bymethods such as PCR, RT-qPCR,
Droplet Digital PCR, or LAMP

195.00

Biotechnology Grade Resins
1431255
AG 1-X2 Resin,biotechnology grade, 100 g

Hydroxide

200–400

75–180755.001431345

AG 1-X4 Resin,biotechnology grade, 100 g

Hydroxide

100–200

63–1501432445

AG 1-X8 Resin,biotechnology grade, 100 g
AG 1-X8 Resin,biotechnology grade, 100 g
AG 50W-X2 Resin,biotechnology grade, 100 g
AG 50W-X4 Resin,biotechnology grade, 100 g
AG 50W-X8 Resin,biotechnology grade, 100 g
AG 50W-X8 Resin,biotechnology grade, 100 g
AG 11 A8 Resin,biotechnology grade, 100 g
AG 501-X8 Resin,biotechnology grade, 100 g
AG 501-X8(D) Resin,biotechnology grade, 100 g
Chelex 100 Resin,biotechnology grade, 100 g
Bio-Rex 70 Resin,biotechnology grade, 100 g
Bio-Rex 70 Resin,biotechnology grade, 100 g
Bio-Beads SM-2, 25 g
Bio-Beads SM-2, 100 g

Hydroxide

100–200

106–180

Separation of small peptides,
nucleotides, and large metal
complexes
Separation of organic acids,
nucleotides, phosphoinositides,
and other anions
Separation of inorganic andorganic anions with MW <1,000

Hydroxide

200–400

45–106

Hydrogen

100–200

106–300

Hydrogen

200–400

75–150Hydrogen100-200

106-250

Hydrogen

200–400

63–150Selfadsorbed
H+ + OH–

50–100

180–425

Removal of ionic compounds

400.00

20–50

300–1,180

Deionization

400.00

H+ + OH–

20–50

300–1,180

Sodium100–200

150–300

Chelation of divalent cations

415.00Sodium50–100150–300

Separation of cationic proteins
and amines

400.00Sodium200–400

45–75

—
—

—
—

—
—

14324461435241
143534114354411435451
1437834
1437424
1437425
1432832
1435832
143585215289201523920

98 | Chromatography: Laboratory and Process Separations

445.00825.00460.00

Separation of peptides,
nucleotides, and cations
Separation of amino acids,
nucleotides, and cations
Separation of amino acids
and cations

400.00400.00400.00400.00400.00400.00
Detergent removal
Detergent removal

125.00465.00In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Mixed-Mode Chromatography

bio-rad.com/mixedmode

Mixed-Mode Chromatography
Mixed-mode media offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules.
These products can be used at any stage in a process from initial capture to final polishing.

See Also

Nuvia Mixed-Mode Resins
Nuvia aPrime 4A hydrophobic anion exchange resin andNuvia cPrime hydrophobic cation exchange resin are partof Bio-Rad’s family of mixed-mode purification products.
These resins are designed for process-scale purification ofa wide variety of therapeutic proteins.
Nuvia mixed-mode resins deliver value by providing:■

Unique selectivity■

Simple method development■

 arge design space for capture and elution of a variety
Lof biotherapeutic proteins■

High recovery of target protein■

Mechanical and chemical stability

Nuvia cPrime Hydrophobic Cation Exchange Resin
Nuvia cPrime resin’s unique selectivity allows method
developers to use hydrophobic and cation exchangeinteraction modes to achieve effective purification.
Importantly, the resin has a large design space for binding
and elution, allowing for the development of highly robust
methods in a commercial manufacturing setting. Nuvia
cPrime is built on a rigid, mechanically and chemicallystable macroporous base matrix with a particle size
optimized to deliver exceptional flow properties, fast masstransfer, and stability.

Foresight prepacked
plates and columns:
pages 90, 132, 173.
EconoFit prepacked
columns:
page 127.

For More Information
Web: bio-rad.com/nuvia
bio-rad.com/aprime
Request or download bulletins: 6241, 6242, and 6418

Nuvia aPrime 4A Hydrophobic Anion Exchange Resin
Nuvia aPrime 4A resin is designed to provide optimal
efficiency and productivity for process-scale purificationof therapeutic proteins. The resin's ligand density andhydrophobicity are engineered to facilitate selective andreversible binding of target molecules for high purity andyield. Nuvia aPrime 4A resin can be used for both capture
and polish purification. It offers a wide design space andstraightforward method development to purify even themost complex molecules.
Properties of Nuvia aPrime 4A and Nuvia cPrime
Property

Nuvia aPrime 4A

Nuvia cPrime

Functional groupMedian particle size
Dynamic binding capacity
Ligand density
Recommended linear flow rate range
pH stability**

Aromatic hydrophobic anion exchanger
50 µm≥50 mg/ml at 300 cm/hr*
100 ± 20 µeq/ml
50–300 cm/hr
Short term: 2–14

Chemical stability†

1 N NaOH, 1 M HCl, 25% acetic acid, 8 M urea,
6 M guanidine HCl, 3 M NaCl, 1% Triton X-100,
20–70% ethanol, 30% isopropanol

Shipping solution
Storage conditions

20% ethanol + 1 M NaCl
20% ethanol

Aromatic hyrdophobic cation exchanger
70 µmhlgG: >40 mg/ml (at 10% breakthrough, 300 cm/hr)
123 ± 20 µeq/ml
50–600 cm/hr
1–14 short term
3–13 long term
25% acetic acid, 8 M urea, 6 M guanidine-HCI,
1 N NaOH, 1 M HCI, 6 M potassium thiocyanate,
3 M sodium chloride, 1% Triton X-100, 2% SDS,
20–70% ethanol, 30% isopropyl alcohol
20% ethanol, 30 mM Na2SO3
20% ethanol or 0.1 N NaOH

* 10% breakthrough capacity determined with 1.0 mg/ml of an acidic monoclonal antibody (mAb; pI ~6.9) in 20 mM NaPO4, pH 7.8.
** No significant change in ligand density after 200 hr contact at 22°C.
No significant change in ligand density after 1 week contact at 22°C.

†

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 99

Chromatography Media
Mixed-Mode Chromatography

bio-rad.com/mixedmodeOrdering Information
Description
Nuvia cPrime Media
Nuvia cPrime Media, 25 ml
Nuvia cPrime Media, 100 ml
Nuvia cPrime Media, 500 ml
Nuvia cPrime Media, 1 L
Nuvia cPrime Media, 5 L
Nuvia cPrime Media, 10 L
EtOH, ethanol; BnOH, benzyl alcohol.

Catalog #Catalog #20% EtOH Shipping Solution
1563401
1563402156-3403
156-3404
156-3405
156-3406

2% BnOH Shipping Solution
12003716
12003717
12003718
12003719
12003720
12003721

Ordering Information
Catalog #Description

Nuvia cPrime Foresight Plates, Columns, and RoboColumn Units
732-4705*
Foresight Nuvia cPrime Plates, 20 µl
732-4722
Foresight Nuvia cPrime Column, 1 ml
732-4742
Foresight Nuvia cPrime Column, 5 ml
732-4807**
Foresight Nuvia cPrime RoboColumn Unit, 200 µl
732-4808**
Foresight Nuvia cPrime RoboColumn Unit, 600 µl

551.25141.95278.22231.53277.83Nuvia aPrime 4A Media
12007397
Nuvia aPrime 4A Media, 25 ml
12007396

Nuvia aPrime 4A Media, 100 ml

12007379

Nuvia aPrime 4A Media, 500 ml

12007380

Nuvia aPrime 4A Media, 5 L

12007391

Nuvia aPrime 4A Media, 10 L

Foresight Nuvia aPrime 4A Plates, Columns, and RoboColumn Units
12007411*
12007392

Foresight Nuvia aPrime 4A Plates, 20 µl
Foresight Nuvia aPrime 4A Column, 1 ml

12007393

Foresight Nuvia aPrime 4A Column, 5 ml

12007394**

Foresight Nuvia aPrime 4A RoboColumn, 200 µl

12007395**

Foresight Nuvia aPrime 4A RoboColumn, 600 µl

EconoFit Nuvia aPrime 4 and cPrime Columns***
12009281

EconoFit Nuvia cPrime Column, 1 x 1 ml, 7 x 25 mm

12009280

EconoFit Nuvia aPrime 4A Column, 1 x 1 ml, 7 x 25 mm

EconoFit Column Packs
17007891

EconoFit mAb Purification Pack, includes 1 ml column each of Nuvia S, Nuvia Q,
Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, and CHT XT

17007894

EconoFit Aggregate Clearance Pack, includes 1 ml column each of Nuvia cPrime,
Nuvia aPrime 4A, Nuvia HR-S, CHT XT

17007893

EconoFit Large Biomolecule Purification Pack, includes 1 ml column each of Nuvia Q,
Nuvia HP-Q, Nuvia cPrime, Nuvia aPrime 4A, CHT Tpe II (40 μm), and CHT XT* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
*** Maximum pressure 45 psi.

100 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Mixed-Mode Chromatography

bio-rad.com/mixedmodeHydroxyapatite (Ca 5(PO4)3OH) 2 and fluoroapatite Ca10(PO4) 6F2 are forms of calcium phosphate that offer unique selectivities
and often separate biomolecules that appear homogeneous when other chromatographic and electrophoretic techniques
are used. Hydroxyapatite and fluoroapatite chromatography can be used at any stage from initial capture to final polishing.
See Also

Ceramic Hydroxyapatites
CHT ceramic hydroxyapatite is a spherical, macroporous,and chemically pure form of hydroxyapatite that has been
sintered at high temperatures to yield a physically andchemically robust support. It gives consistent, reproducible
results over many cycles at scales up to 1.8 m, making
CHT ceramic hydroxyapatite ideal for large-scale
bioprocess applications. It has unique separation
properties and unmatched selectivity and resolution.
Applications include the purification of enzymes, antibody
fragments, bispecific antibodies, and monocolonal andpolyclonal antibodies of various classes; separation ofsupercoiled DNA from linear duplexes as well as singlestranded from double-stranded DNA; and efficient isolationand purification of viruses and virus-like particles. CHT
ceramic hydroxyapatite is available in three distinct versions:Type I, XT, and Type II. The three types differ in their pore
diameters and can differ slightly in protein separation,offering flexibility to select the type that provides maximum

benefit in a given separation. CHT Type I and XT, with theirsmaller pore diameter and larger surface area, have higher
binding capacities than CHT Type II. CHT Type II with its
larger pore diameter provides higher capacity for large
molecules, such as IgM and virus particles.
■

High throughput■

High physical and chemical stability■

Unmatched selectivity■

Single-step clearance of impurities and aggregates■

Rapid and simple column packing

Process-Scale
Prepacked
Chromatography
Columns — GMP Ready,page 175
Foresight prepacked
plates and columns:
pages 132, 173.
EconoFit columns:
page 127
Bio-Scale CHT Type I
columns: page 136.

For More Information
Web: bio-rad.com/cht
bio-rad.com/chtxt
bio-rad.com/ForesightProColumn
Download bulletin: 5853
Request or download bulletins: 5667, 5709, 6086, 6549, and 7080

Specifications
CHT Type I

CHT Type II

CHT XT

Functional groups

Ca 2+, PO43–, OH –Ca 2+, PO43–, OH –Ca 2+, PO43–, OH –Observed dynamic binding
capacity, lysozyme

25–40 mg lysozyme/g*

12.5–20 g lysozyme/g*

17.4–24.8 mg lysozyme/g*

Median particle size, µm

20, 40, 80

20, 40, 80

40

Tap settled density*(g/ml tap settled bed)

0.63 g/ml

0.63 g/ml

0.67 g/ml* Under ideal conditions.
Properties of CHT
Property

CHT Type I

CHT Type II

CHT XT

Observed dynamic binding
capacity, IgG*

25–60 mg IgG/ml

15–25 mg IgG/ml>60 mg/ml mAb G** (pI 9.2)

Typical linear flow rate

50–400 cm/hr

50–400 cm/hr

50–400 cm/hr

pH stability

6.5–14

6.5–14

6.5–14

Regeneration

0.4 M sodium phosphate
pH 7; 1 M trisodium phosphate,
pH 11–12

0.4 M sodium phosphate
pH 7; 1 M trisodium phosphate,
pH 11–12

0.4 M sodium phosphate, pH
7.0–7.5; 1 M trisodium phosphate,
pH 11–12

Autoclavability (bulk)

121°C, 20 min in phosphatebuffer, pH 7

121°C, 20 min in phosphatebuffer, pH 7

121°C, 20 min in phosphatebuffered saline, pH 7

Sanitization

1–2 N NaOH

1–2 N NaOH

1–2 N NaOH

Recommended column storage

0.1 N NaOH

0.1 N NaOH

0.1 N NaOH

* At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7, in a 0.5 x 9.5 cm column.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 101

Chromatography Media
Mixed-Mode Chromatography

bio-rad.com/mixedmodeOrdering Information
Description

Type I

Type IIXTCHT Ceramic Hydroxyapatite, 20 µm
20 µm particle size, 10 g
20 µm particle size, 100 g
20 µm particle size, 1 kg*

1582000
1570020157-0021

1582200
1572000
157-2100———

CHT Ceramic Hydroxyapatite, 40 µm
40 µm particle size, 10 g
40 µm particle size, 100 g
40 µm particle size, 1 kg*
40 µm particle size, 5 kg*

1584000
1570040
157-0041
157-0045

1584200
1574000
157-4100
157-4500

12002457
12002454
12002456
12002455

CHT Ceramic Hydroxyapatite, 80 µm
80 µm particle size, 10 g
80 µm particle size, 100 g
80 µm particle size, 1 kg*
80 µm particle size, 5 kg*

1588000
1570080
157-0081
157-0085

1588200
1578000
157-8100
157-8500————

Foresight CHT Plates, Columns, and RoboColumn Units
Foresight CHT Plates, 40 µm, 20 µl**
Foresight CHT Column, 40 µm, 1 ml
Foresight CHT Column, 40 µm, 5 ml
Foresight CHT RoboColumn Unit, 40 µm, 200 µl***732-4716
732-4735
732-4755
732-4822732-4718
732-4736
732-4756
732-4825

12003151
12003150
12003149
12003152

Foresight CHT RoboColumn Unit, 40 µm, 600 µl***732-4823

732-4826

12003148

Catalog #Description

Foresight Pro Column, CHT Type I, 40 μm†
12014659
Foresight Pro Column, CHT Type I, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014660
Foresight Pro Column, CHT Type I, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014671
Foresight Pro Column, CHT Type I, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014672
Foresight Pro Column, CHT Type I, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014673
Foresight Pro Column, CHT Type I, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014674
Foresight Pro Column, CHT Type I, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014675
Foresight Pro Column, CHT Type I, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014676
Foresight Pro Column, CHT Type I, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014677
Foresight Pro Column, CHT Type I, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014678
Foresight Pro Column, CHT Type I, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014679
Foresight Pro Column, CHT Type I, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014680
Foresight Pro Column, CHT Type I, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014681
Foresight Pro Column, CHT Type I, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014682
Foresight Pro Column, CHT Type I, 40 μm, 17 L, 33 cm ID x 20 cm length
Foresight Pro Column, CHT Type II, 40 μm†
12014697
Foresight Pro Column, CHT Type II, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014698
Foresight Pro Column, CHT Type II, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014699
Foresight Pro Column, CHT Type II, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014700
Foresight Pro Column, CHT Type II, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014701
Foresight Pro Column, CHT Type II, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014702
Foresight Pro Column, CHT Type II, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014703
Foresight Pro Column, CHT Type II, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014704
Foresight Pro Column, CHT Type II, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014705
Foresight Pro Column, CHT Type II, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014706
Foresight Pro Column, CHT Type II, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014707
Foresight Pro Column, CHT Type II, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014708
Foresight Pro Column, CHT Type II, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014709
Foresight Pro Column, CHT Type II, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014710
Foresight Pro Column, CHT Type II, 40 μm, 17 L, 33 cm ID x 20 cm length

continues

102 | Chromatography: Laboratory and Process Separations

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Chromatography Media
bio-rad.com/mixedmode

Mixed-Mode Chromatography

Ordering Information
Catalog #Description

Foresight Pro Column, CHT XT, 40 μm†
12014725
Foresight Pro Column, CHT XT, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014726
Foresight Pro Column, CHT XT, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014727
Foresight Pro Column, CHT XT, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014728
Foresight Pro Column, CHT XT, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014729
Foresight Pro Column, CHT XT, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014730
Foresight Pro Column, CHT XT, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014731
Foresight Pro Column, CHT XT, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014732
Foresight Pro Column, CHT XT, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014733
Foresight Pro Column, CHT XT, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014734
Foresight Pro Column, CHT XT, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014735
Foresight Pro Column, CHT XT, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014736
Foresight Pro Column, CHT XT, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014737
Foresight Pro Column, CHT XT, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014738
Foresight Pro Column, CHT XT, 40 μm, 17 L, 33 cm ID x 20 cm length
EconoFit CHT Columns††
12009255
EconoFit CHT Type I, 40 µm Column, 1 x 1 ml, 7 x 25 mm
12009253
EconoFit CHT Type I, 40 µm Column, 1 x 5 ml, 16 x 25 mm
12009254
EconoFit CHT Type I, 40 µm Columns, 5 x 5 ml, 16 x 25 mm
12009256
EconoFit CHT Type I, 80 µm Column, 1 x 1 ml, 7 x 25 mm
12009259
EconoFit CHT Type II, 40 µm Column, 1 x 1 ml, 7 x 25 mm
12009257
EconoFit CHT Type II, 40 µm Column, 1 x 5 ml, 16 x 25 mm
12009258
EconoFit CHT Type II, 40 µm Columns, 5 x 5 ml, 16 x 25 mm
12009260
EconoFit CHT Type II, 80 µm Column, 1 x 1 ml, 7 x 25 mm
12009261

EconoFit CHT XT Column, 1 x 1 ml, 7 x 25 mm

EconoFit Column Packs
17007881
EconoFit CHT Screening Pack, includes 1 ml column each of CHT Type I (40 μm),
CHT Type II (40 μm), CHT XT, and CFT Type II (40 μm)
17007891
EconoFit mAb Purification Pack, includes 1 ml column each of Nuvia S, Nuvia Q,
Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, and CHT XT
17007894
EconoFit Aggregate Clearance Pack, includes 1 ml column each of Nuvia cPrime,
Nuvia aPrime 4A, Nuvia HR-S, and CHT XT
17007893
EconoFit Large Biomolecule Purification Pack, includes 1 ml column each of Nuvia Q,
Nuvia HP-Q, Nuvia cPrime, Nuvia aPrime 4A, CHT II, 40 μm, and CHT XT
Larger volumes and special packaging for industrial applications are available on request.
* Use the resin calculator at bio-rad.com/calculator to calculate resin for packed bed volumes.
** Package size: 2 x 96-well plates.
*** Package size: 1 row of 8 columns.
†
Foresight Pro Prepacked CHT Chromatography Columns — GMP Ready, additional details on page 175.
† † Maximum pressure 72 psi.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 103

Chromatography Media
Mixed-Mode Chromatography

bio-rad.com/mixedmode

Ceramic Fluoroapatites
MPC Ceramic Hydroxyfluoroapatite
MPC ceramic hydroxyfluoroapatite is a composite ofhydroxyapatite and fluoroapatite with 25% of the hydroxyl
groups substituted with fluoride, (Ca10(PO4)6(OH)1.5(F)0.5).
MPC complements our line of ceramic apatite mixed-mode
chromatography media with its unique separation properties
for biomolecule purification.
CFT Ceramic Fluoroapatite
CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid sphericalmacroporous media used in the purification of biologicallysignificant compounds. CFT is a composite of fluoroapatiteand hydroxyapatite prepared by chemically convertinghydroxyapatite nanocrystals to fluoroapatite with a fluorine
reagent. CFT can be used under stringent chromatographyrequirements to separate acidic proteins requiring bufferedconditions as low as pH 5.6.
For More Information
Web: bio-rad.com/mpc
Web: bio-rad.com/cft
Download bulletins: 3111 and 5853 (CFT); 6432 (MPC)
Request or download bulletin: 6086 (MPC)

Specifications
MPC
Functional groups
Observed dynamic binding
capacity, lysozyme
Median particle size, µm
Tap settled density*(g/ml tap settled bed)

MPC Type I
Ca2+, PO43–,
OH –, F–
≥25 mglysozyme/g
400.72 g/ml

CFT Type II
Ca 2+, PO43–, F–
14–21.5 mg
lysozyme/g
400.86 g/ml* Under ideal conditions.
Properties of MPC and CFT
Property
MPC Type I
Observed dynamic binding
capacity, lgG
Typical linear flow rate
range
pH stability
Autoclavability (bulk)

Sanitization
Recommended column
storage

CFT Type II

25–50 mg lgG/ml*

33 mg lgG/ml

50–400 cm/hr

50–400 cm/hr

6.5–14121ºC, 20 min in
phosphate buffer,
pH 7
1–2 N NaOH

5–14
121°C, 20 min in
phosphate buffer,
pH 7
1–2 N NaOH

0.1 N NaOH

0.1 N NaOH

* 40 μm particles, 300 cm/hr, 5 mM sodium phosphate,
25 mM NaCl, pH 6.5.

Ordering Information
Catalog #Description

Price, U.S. $MPC Ceramic Hydroxyfluoroapatite, 40 µm
1580200

40 µm particle size, 10 g

1570200

40 µm particle size, 100 g

157-0201

40 µm particle size, 1 kg

157-0205

40 µm particle size, 5 kg

MPC Foresight Plates, Columns, and RoboColumn Units
732-4785

Foresight MPC Type I Plates, 20 μl*

732-4737

Foresight MPC Type I Column, 40 µm, 1 ml

732-4757

Foresight MPC Type I Column, 40 µm, 5 ml

732-4828

Foresight MPC Type I RoboColumn Unit, 40 µm, 200 µl**732-4829

Foresight MPC Type I RoboColumn Unit, 40 µm, 600 µl**

CFT Ceramic Fluoroapatite
1585200
40 µm particle size, 10 g
1575000
40 µm particle size, 100 g
157-5100
40 µm particle size, 1 kg
157-5500
40 µm particle size, 5 kg

585.001,660.00
InquireInquireEconoFit MPC and CFT Columns***
12009279
EconoFit MPC I-40 Column, 1 x 1 ml, 7 x 25 mm
12009252
EconoFit CFT Type II, 40 µm Column, 1 x 1 ml, 7 x 25 mm
12009240
EconoFit CFT Type II, 40 µm Column, 1 x 5 ml, 16 x 25 mm
12009251
EconoFit CFT Type II, 40 µm Columns, 5 x 5 ml, 7 x 25 mm
17007881
EconoFit CHT Screening Pack, includes 1 ml column each of CHT Type I (40 μm),
CHT Type II (40 μm), CHT XT, and CFT Type II (40 μm)* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
*** Maximum pressure 72 psi.
Use the resin calculator at bio-rad.com/calculator to calculate resin for packed bed volumes.

104 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Mixed-Mode Chromatography

bio-rad.com/mixedmode

See Also

Bio-Gel Hydroxyapatite HT and HTP

CHT ceramichydroxyapatite:
page 101.

Hydroxyapatite (Ca 5(PO4 )3OH)2 is a form of calcium phosphateused in the chromatographic separation of biomolecules.
Bio-Gel crystalline hydroxyapatite media is compatible witha wide range of aqueous buffers and organic modifiers andcan be sanitized in up to 1 M NaOH. Typical pH tolerance is>6.8, however it can be used at 5.5 in single-use applications.
Bio-Gel hydroxyapatite can be autoclaved in buffers thatmaintain a pH above 7 during the autoclaving cycle.
■■■

 io-Gel HT media — shipped in 10 mM sodiumB
phosphate, pH 6.8 buffer containing 0.02% NaN3. The flowrate range is 25–100 cm/hr at 10 cm bed height in a gravitypacked column. Bio-Gel HT media has a shelf life of at least
one year when stored at 4°C in the shipping buffer io-Gel HTP powder — the dry form of Bio-Gel HT media.
B
When hydrated, it should be stored similarly to Bio-Gel HT
media; the flow rate range is similar to Bio-Gel HT media
 NA grade Bio-Gel HTP powder — a smaller particle
D
size version of Bio-Gel HTP powder, it exhibits higher
capacity for biomolecules. It is generally used forsingle- and double-stranded DNA separations.
It has an increased capacity for RNA, making it usefulfor DNA-RNA hybridization studies. Its flow rate is limited
in gravity feed columns, but it can be used in mediumpressure columns to enhance flow rate

Bio-Scale CHT Type I
columns: page 136.

Bio-Gel HT and HTP

Properties of Bio-Gel HT and HTP
Bio-GelHT

Bio-GelHTP

Bio-Gel HTP(DNA Grade)

Flow rate,* cm/hr

>25

>25

>5

BSA adsorbed,**mg per dry gram

10

10

10

Calf thymus DNA
adsorbed, μg per
dry gram

>500

>500

>800

Hydrated volume—

2–3 ml/g

2–3 ml/g

Property*		Flow rate determined on a 1.5 x 10 cm column with 40 cm
hydrostatic pressure.
** Batch-wise uptake.
For More Information
Web: bio-rad.com/biogelHTandHTP
Download bulletin: LIT217

Ordering Information
Catalog #Description

1300150130-0151
1300420130-0421
130-0425
1300520

Bio-Gel HT Hydroxyapatite, hydrated, 250 ml
Bio-Gel HT Hydroxyapatite, hydrated, 500 ml
Bio-Gel HTP Hydroxyapatite, powder, 100 g
Bio-Gel HTP Hydroxyapatite, powder, 1 kg
Bio-Gel HTP Hydroxyapatite, powder, 5 kg
Bio-Gel HTP Hydroxyapatite, DNA grade, 100 g

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $315.00545.00345.00InquireInquire620.00Chromatography: Laboratory and Process Separations | 105

Chromatography Media
Affinity Chromatography

bio-rad.com/affinityresin

Affinity Chromatography
Nuvia IMAC Resin
Nuvia IMAC resin is a high-capacity, next-generation
metal chelate affinity chromatography resin. Nuvia IMAC
delivershigh binding capacity over a range of pH and flow
rates, providing wide experimental design space for many
applications. Immobilized metal affinity chromatography(IMAC) is a powerful purification technique that relieson the affinity that the histidine tag has for immobilized
transition metals. Nuvia IMAC resins contain nitrilotriacetic
acid (NTA) as the chelating ligand for di- or trivalent
metal ions. Its chemical structure allows highly selective
binding of recombinant histidine-tagged proteins whencharged with Ni 2+ or other transition metals, such as Zn2+
or Cu2+. The optimized particle size and high porosity
allow operation at increasingly higher flow rates withoutcompromising binding capacity, resulting in superior masstransfer characteristics.

■■

 ptimized particle size for high flow rate andOlow backpressure
Superior dynamic binding capacity at high flow rates
 obust base matrix withstands repeated clean-in-place/R
recharging cycles■

Excellent recovery and reproducibility■

Full regulatory support■

Ultra-high capacity at high flow rates■

pH stability across broad range

For More Information
Web: bio-rad.com/nuviaIMACresin
Download bulletin: 6859

106 | Chromatography: Laboratory and Process Separations

60
50
40
30
20
10
0
50

100

150
200
250
Linear velocity, cm/hr

300

350

Comparison of dynamic binding capacity vs. flow velocity of
Nuvia IMAC resin ( ), agarose IMAC resin A (n), and agarose IMAC
resin B ( ). BT, breakthrough.
50
3

40
30

2

20

1

10
0

75

125

175

225

275

325

375

0
425

Column pressure (net), bar■ imple scale-up from laboratory to bioprocessS
manufacturing scale

Dynamic binding capacity, 10% BT■

Column pressure (net), psi

Nuvia IMAC is available in two forms: uncharged andcharged with Ni2+. The uncharged form can be charged
with the metal ion of your choice for even greater
purification flexibility. Nuvia IMAC can be used at any
scale from laboratory to manufacturing, providing robust,
reproducible, and efficient purifications each time. Nuvia
IMAC resin delivers value and flexibility by providing users:

Nuvia IMAC Resins and Columns

Linear velocity, cm/hr

Flow performance of Nuvia IMAC resin. 20 x 20 cm column,
1.2 compression factor.

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Affinity Chromatography

bio-rad.com/affinityresin

Ordering Information
Description

Nuvia IMAC Ni-Charged Resin, 25 ml
Nuvia IMAC Ni-Charged Resin, 100 ml
Nuvia IMAC Ni-Charged Resin, 500 ml
Nuvia IMAC Ni-Charged Resin, 5 L
Nuvia IMAC Ni-Charged Resin, 10 L
Nuvia IMAC Uncharged Resin, 25 ml
Nuvia IMAC Uncharged Resin, 500 ml
Nuvia IMAC Uncharged Resin, 5 L
Nuvia IMAC Uncharged Resin, 10 L

Catalog #Catalog #20% EtOH
Shipping Solution
7800800
7800801
780-0802
12003233
12002782
12018135
12018162
12004040
12004039

2% BnOH
Shipping Solution
12003722
12003723
12003724
12003725
12003726

EtOH, ethanol; BnOH, benzyl alcohol.
Ordering Information
Catalog #Description

Price, U.S. $Nuvia IMAC Foresight Plates, Columns, and RoboColumn Units
12004035*
Foresight Nuvia IMAC Ni-Charged Plates, 20 µl1,010.00
12004038
Foresight Nuvia IMAC Ni-Charged Column, 1 ml1,010.00
12004037
Foresight Nuvia IMAC Ni-Charged Column, 5 ml1,010.00
12004051**
Foresight Nuvia IMAC Ni-Charged RoboColumn Unit, 200 µl 1,010.00
12004052**
Foresight Nuvia IMAC Ni-Charged RoboColumn Unit, 600 µl Inquire
EconoFit Nuvia IMAC Columns* and Application Pack***
12009288
EconoFit Nuvia IMAC Column, Ni-charged, 1 x 1 ml, 7 x 25 mm
12009286
EconoFit Nuvia IMAC Column, Ni-charged, 1 x 5 ml, 16 x 25 mm
12009285
EconoFit Nuvia IMAC Columns, Ni-charged, 5 x 1 ml, 1 ml, 7 x 25 mm
12009287
EconoFit Nuvia IMAC Columns, Ni-charged, 5 x 5 ml, 16 x 25 mm
12009289
EconoFit Nuvia IMAC Column, Uncharged, 1 x 1 ml, 7 x 25 mm
17007882
EconoFit IMAC and Desalting Pack, includes 5 ml column each of Bio-Gel P-6 Desalting and Nuvia IMAC
His Antibody
6200203

His Antibody, monoclonal antibody against histidine-tagged proteins, 1 mg/ml in phosphate buffered saline (pH 7.4)
with 0.05% NaN3

* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
*** Maximum pressure 45 psi.
Ordering Information
Catalog #Description

Price, U.S. $See Also

Affinity Purification Buffers and Kits
6200205
6200206
6200207
6200207

2x Native IMAC Lysis Buffer, 125 ml
2x Native IMAC Wash Buffer 1, 125 ml
2x Native IMAC Wash Buffer 2, 100 ml
Native IMAC Buffer Kit, includes native IMAC lysis, wash, and elution buffers

In the U.S., call toll free at 1-800-424-6723

78.00
52.00
45.00

Affinity media
selection guide:
page 86.

Chromatography: Laboratory and Process Separations | 107

Chromatography Media
Affinity Chromatography

bio-rad.com/affinityresin

Profinity IMAC Resin
Profinity IMAC resins are affinity chromatography
supports for the purification of recombinant histidinetagged proteins. Profinity IMAC resins, based on
UNOsphere beads, contain iminodiacetic acid (IDA)
as the chelating ligand for di- or trivalent metal ions.
Its chemical structure allows highly selective binding ofrecombinant histidine-tagged proteins when charged
with Ni2+ or other transition metals such as Zn2+ or Cu2+.
They offer high capacity at high flow rates and can beused under either nondenaturing or denaturing conditions.
The resins are suitable for purification using liquid
chromatographic instrumentation, gravity-flow columns,
or spin columns. The resin is available in two forms:
uncharged and precharged with Ni 2+. The uncharged
form can be charged with the metal ion of your choicefor even greater purification flexibility.

Profinity IMAC Resins

Features of Profinity IMAC resins include:
EconoFit Columns■

Optimal ligand density for higher purity of target protein■

Suitable for a wide molecular weight■■

Superb mechanical strength■■ xcellent pressure-flow properties for high maximumE
operating pressures and flow rates, allowing rapid
purification, column cleaning, and re-equilibration

Stability from pH 1–14
 ompatibility with denaturing agents, detergents,Cand reducing agents

For More Information
Web: bio-rad.com/profinityIMAC

Ordering Information
Catalog #Description

Profinity IMAC Resins
1560121
Profinity IMAC Uncharged Resin, 10 ml
1560123
Profinity IMAC Uncharged Resin, 50 ml
1560131
Profinity IMAC Ni-Charged Resin, 10 ml
1560133
Profinity IMAC Ni-Charged Resin, 25 ml
1560135
Profinity IMAC Ni-Charged Resin, 100 ml
156-0137
Profinity IMAC Ni-Charged Resin, 500 ml

Properties (all listed products)• Stable from pH 1 to 14• Strong bead matrix for gravity flow

Price, U.S. $81.00
350.00100.00245.00905.00InquireEconoFit Profinity IMAC Columns*
12009298
EconoFit Profinity IMAC Ni-Charged Columns, 5 x 1 ml
12009299
EconoFit Profinity IMAC Ni-Charged Column, 1 x 5 ml
12009300
EconoFit Profinity IMAC Ni-Charged Columns, 5 x 5 ml
His Antibody
6200203

His Antibody, monoclonal antibody against histidine-tagged proteins, 1 mg/ml in phosphate buffered saline(pH 7.4) with 0.05% NaN3

Affinity Purification Buffers and Kits
6200205
2x Native IMAC Lysis Buffer, 125 ml
6200206
2x Native IMAC Wash Buffer 1, 125 ml
6200207
2x Native IMAC Wash Buffer 2, 100 ml
6200239
Native IMAC Buffer Kit, includes native IMAC lysis, wash, and elution buffers* Maximum pressure 72 psi.
Larger volumes and special packaging for industrial applications are available on request.

108 | Chromatography: Laboratory and Process Separations

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Chromatography Media
Affinity Chromatography

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Profinity Epoxide Resin
Profinity epoxide resin is an activated affinity
chromatography support for the immobilization of
biomolecules. Profinity epoxide is a useful support for theimmobilization of ligands that contain nucleophiles suchas amino, thiol, or hydroxyl groups. These groups couple
to the epoxy groups on the resin, which is then used for the
purification of proteins, carbohydrates, or DNA.
Profinity epoxide resin is based on UNOsphere beads,
which have an open pore structure. The open pore
structure allows coupling of large ligands for the purificationof large targets such as Protein A, recombinant proteins
containing MBP, and calmodulin. It is supplied as a dry
powder (1 g of powder gives ~8 ml final volume).
For More Information
Web: bio-rad.com/profinityepoxide

Profinity Epoxide Resins

Ordering Information
Catalog #Description

1560200
1560201

Profinity Epoxide Resin, 5 g
Profinity Epoxide Resin, 25 gPrice, U.S. $120.00575.00

Profinity GST Columns and Reagents
Profinity GST resin is an affinity chromatography support
for the purification of recombinant GST-tagged proteins.
It is available in the convenient prepacked EconoFit
columns format.
Histidine and GST Purification E. coli Control Lysate
The histidine and GST purification E. coli control lysate is alyophilized, dual-tagged 51 kD target protein that is meant
to eliminate concerns over variability in purification buffer
solutions or in the purification matrix itself. The control
lysate facilitates system setup and initial purification.
Glutathione Reagent
Powdered glutathione reagents are available for use with GST
applications. The glutathione pack is essential for eluting
GST fusion proteins from immobilized glutathione resins.

Histidine and GST
Purification E. coli
Control Lysate

Glutathione

Histidine and GST Antibodies

For More Information
Web: bio-rad.com/econofit

Ordering Information
Catalog #Description

12009296
12009295
12009297
6200204

EconoFit Profinity GST Column, 1 x 5 ml
EconoFit Profinity GST Columns, 5 x 1 ml
EconoFit Profinity GST Columns, 5 x 5 ml
GST Antibody, monoclonal antibody against GST-tagged proteins, 1 mg/ml in phosphate buffered saline (pH7.4) with 0.05% NaN3
Glutathione, 1.23 g, powdered reagent, for denaturing GST applications

6200202

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Chromatography: Laboratory and Process Separations | 109

Chromatography Media
Affinity Chromatography

bio-rad.com/affinity

Profinity eXact Resin
Profinity eXact Purification Resin, Prepacked Columns,
and Mini Spin Columns
The Profinity eXact purification resin consists of a highly
engineered subtilisin protease conjugated to an agarosebased matrix. Purity of the eluted protein using thisresin is typically higher than that for other affinity-tag
systems due to the specific recognition of subtilisin
for its prodomain sequence (KD <100 pM).
The resin can be packed into different column formats,including low- to medium-pressure columns, gravityflow columns, and mini spin columns, offering added
purification flexibility. EconoFit columns are available in
1 and 5 ml volumes. Mini spin columns contain 0.1 ml
resin. Additional advantages of Profinity eXact purification
resin include:■Purification and processing of fusion-tagged proteinsin a single step■On-column cleavage in as little as 30 min■

No protease addition is required■Precise cleavage at N-terminus to generate native

protein sequence
Resin is supplied in 100 mM sodium phosphate (pH 7.2)
containing 0.02% sodium azide. It is also available prepacked
in 1 and 5 ml columns and in mini spin columns.

Profinity eXact Resin Bottle and Columns

Profinity eXact pPAL7 Supercoiled Expression
Vector Kit
The pPAL7 supercoiled plasmid expression vector isused for routine cloning of target DNA sequences using
a conventional restriction digest cloning strategy.
For More Information
Download bulletin: 5655

Resin Specifications
Functional ligand		
Subtilisin protease, 27.8 kD
Base bead			
6% agarose bead
50% suspension in 100 mM sodium phosphate (pH 7.2), 0.02% sodium azide
Form 			60–160 µm
Particle size range		>3 mg tag-free protein/ml resin
Dynamic binding capacity*
1,000 cm/hr at 25°C
Recommended linear flow rate
pH stability			2–13
Common reagents, including detergents, reducing agents, buffering agents, and additives
Chemical compatibility		
Storage			4°C
>1 year at 4°C
Shelf life in 20% ethanol
Operational temperature
4–40°C* Dynamic binding capacity determination of a 40 kD maltose binding protein.
Note: Dynamic binding capacity is protein dependent.

Ordering Information
Catalog #Description

1563007

Profinity eXact Mini Spin Columns, includes 10 spin columns, ten 2 ml capped tubes,
and ten 2 ml capless tubes
Profinity eXact pPAL Supercoiled Expression Vector Kit, includes 100 µl of 100 ng/µl vector, 20 reactions
Profinity eXact Purification Resin,* 10 ml
EconoFit Profinity eXact Column,** 1 x 1 ml
EconoFit Profinity eXact Column,** 1 x 5 ml
EconoFit Profinity eXact Columns,** 5 x 1 ml

1563002
1563005
12009292
12009294
12009293

Price, U.S. $165.00215.00555.00* Larger volumes are available on request.
** Maximum pressure 72 psi.

110 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Affinity Chromatography

bio-rad.com/affinitypurification

See Also

UNOsphere SUPrA rProtein A MediaDeveloped for monoclonal antibody capture
chromatography, UNOsphere SUPrA resin provides anideal balance between dynamic binding capacity, flow
properties, and stability. The UNOsphere hydrophilicpolymeric support provides high-quality purifications andbatch-to-batch reproducibility.
Key features include:■■Designed with large pores that result in high dynamic

EconoFit
Columns:
page 127.

binding capacities at fast flow rates■

 ptimized to operate under a wide range of flow ratesO
up to 600 cm/hr

For More Information
Web: bio-rad.com/unospheresupra
Download bulletins: 5728, 5729, and 6053

Built on robust polymeric beads engineered for highmechanical stability, low backpressures, and resistanceto repeated clean-in-place cycles
Properties of UNOsphere SUPrA
Property

Description

Property

Description

Composition

Highly cross-linked polymer

Working pH range

3–11

Particle size range

53–61 µm

LigandRecombinant Protein A

Clean-in-place (CIP)
solutions

6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol

Recommended mobile
phase velocity range

100–600 cm/hr

Temperature stability

2–40ºC

Delivery conditions

50% slurry in 20% ethanol

Storage conditions

2–8ºC

Coupling chemistry

Epoxy

Dynamic binding capacity*

30 ± 3 mg/ml at 150 cm/hour
25 ± 2 mg/ml at 300 cm/hour
20 ± 2 mg/ml at 450 cm/hour(Minimum spec: 20 mg/ml at 300 cm/hour)

Chemical stability**
		
		
		
		

10 mM hydrochloric acid6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)

* 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
** No significant change in chromatographic performance after storage for 24 hr at room temperature.

Ordering Information
Catalog #Description

UNOsphere SUPrA rProtein A Media
1560250
UNOsphere SUPrA, 5 ml
1560218
UNOsphere SUPrA, 25 ml
1560219
UNOsphere SUPrA, 100 ml

Price, U.S. $InquireInquireInquireEconoFit UNOsphere SUPrA Columns
12009321
EconoFit UNOsphere SUPrA Column, 1 x 1 ml
12009323
EconoFit UNOsphere SUPrA Column, 1 x 5 ml
12009322
EconoFit UNOsphere SUPrA Columns, 5 x 1 ml

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 111

Chromatography Media
Affinity Chromatography

bio-rad.com/affinitypurification

Affi-Gel and Affi-Prep Protein A Resins
Chromatography on Affi-Gel and Affi-Prep Protein A resins
yield highly purified immunoglobulins (IgG), selectively
remove IgG prior to analysis of other IgG classes, or adsorb
immune complexes for antigen purification. Protein A binds
to the Fc region of immunoglobulins, especially IgG frommammalian species. Advantages include:■

High purity of IgGs■

High affinity for mammalian IgG■High capacities for mouse IgG1 as well as othersubclasses with MAPS optimized buffer

In addition, Affi-Prep resins offer high linear flow rates up
to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar),and high chemical stability, which allows sanitization with
0.1 M NaOH.
With Affi-Gel and Affi-Prep Protein A resins and MAPS II
buffer, you can purify up to 10 mg of IgG1/ml of media.

This is 8–10 times higher than with standard methods.
In addition, the MAPS process and Affi-Gel Protein A
permit greater binding of mouse IgG1 than does
immobilized Protein G resin.
For More Information
Web: bio-rad.com/affigelproteina
Capacities of Protein A Resins
Immunoglobulin

Affi-Gel Protein A
Capacity, mg/ml

Mouse IgG1
Mouse IgG2a
Mouse IgG2b
Mouse IgG3
Mouse IgM*
Human IgG
Sheep, cow, horse, goat,
rabbit, dog, pig IgG

Affi-Prep Protein A
Capacity, mg/ml

6–10
6–10
6–10
6–10
6–10
15

7–9
7–9
7–9
7–9
7–9
10–12

8–10

7–9

* Approximately 50% of all mouse IgMs bind using the MAPS buffer system.

Ordering Information
Catalog #Description

Price, U.S. $Affi-Prep and Affi-Gel Media
1560006
Affi-Prep Protein A Media, 5 ml
1560005
Affi-Prep Protein A Media, 25 ml
1536153
Affi-Gel Protein A Media, 5 ml
1536154
Affi-Gel Protein A Media, 50 ml
1536155
Affi-Gel Protein A Media, 1 L
1536159
Affi-Gel Protein A MAPS II Kit, includes 5 ml Affi-Gel Protein A media, Affi-Gel Protein A MAPS II buffers,
1 x 10 cm Econo-Column column; enough to purify 500 mg of mouse IgG1
1536161
Protein A MAPS II Binding Buffer, makes 5 L
Prepacked Econo-Pac Columns
7322022
Econo-Pac Protein A Columns, prefilled with Affi-Gel Protein A media, 57322020
Econo-Pac Protein A Kit, 1 x 2 ml Affi-Gel Protein A column, 1 x 10 ml 10DG column, buffers

415.001,965.00
330.00Inquire470.00835.00540.00330.00

EconoFit Affi-Prep Protein A Columns
12009237
12009236

EconoFit Affi-Prep Protein A Column, 1 x 5 ml
EconoFit Affi-Prep Protein A Columns, 5 x 1 ml

112 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Affinity Chromatography

bio-rad.com/affinityresin

Affi-Gel Blue Resin
Affi-Gel Blue resin is a cross-linked agarose bead withcovalently attached Cibacron Blue F3GA dye. The blue dyefunctions as an ionic, hydrophobic, aromatic, or sterically
active binding site in various applications. Affi-Gel Blue
resin is ideally suited for albumin removal (using 50–100mesh) and enzyme purification (using 100–200 mesh).
Proteins and peptides are bound and released with a highdegree of specificity by manipulating the composition of
the eluent buffers.
The resin is available in bottle and column form.

Affi-Gel Blue Gel

For More Information
Web: bio-rad.com/affigelblue

Ordering Information
Catalog #Description

Applications (all listed products)

1537301
1537302
153-7303
12009234
12009235

Affi-Gel Blue Gel, 100 ml, 50–100 meshAffi-Gel Blue Gel, 100 ml, 100–200 meshAffi-Gel Blue Gel, 1 L, 100–200 mesh
EconoFit Affi-Gel Blue Column, 1 x 5 ml
EconoFit Affi-Gel Blue Columns, 5 x 5 ml

• Albumin removal• Enzyme purification

Price, U.S. $545.00545.00545.00DEAE Affi-Gel Blue Resin
DEAE Affi-Gel Blue resin is a bifunctional affinity gel
containing Cibacron Blue F3GA dye covalently attachedto DEAE Bio-Gel A resin. The dye binds albumin,
proteases, and other complement proteins; the DEAE
group binds remaining acidic proteins. Features of
DEAE Affi-Gel Blue resin include:■

Single-step IgG purification from serum; the eluted IgG

contains a small amount of transferrin (samples are
diluted approximately fivefold)■No detectable proteolytic activity in the eluted IgG fraction■

Economical alternative to Protein A affinity chromatography■

Available in bottle and column form

DEAE Affi-Gel Blue Gel

For More Information
Web: bio-rad.com/affigelDEAE

Ordering Information
Catalog #Description

Applications (all listed products), U.S. $1537307
153-7306
153-7308
12009262

DEAE Affi-Gel Blue Gel, 100 ml
DEAE Affi-Gel Blue Gel, 1 L
DEAE Affi-Gel Blue Gel, 5 L
EconoFit DEAE Affi-Gel Blue
Column, 1 x 5 ml
EconoFit DEAE Affi-Gel Blue
Columns, 5 x 5 ml

• Albumin, proteases, and complement protein removal• IgG purification

12009263

In the U.S., call toll free at 1-800-424-6723

650.00165.00165.00165.00560.00Chromatography: Laboratory and Process Separations | 113

Chromatography Media
Affinity Chromatography

bio-rad.com/affinityresin

CM Affi-Gel Blue Resin
CM Affi-Gel Blue resin contains Cibacron Blue F3GA
dye covalently coupled to CM Bio-Gel A resin.
This bifunctional gel binds both albumin and serum
proteases. CM Affi-Gel Blue chromatography provides
a convenient initial step in the purification of serum
proteins. Features of CM Affi-Gel Blue resin include:■Rapid removal of ≥90% of albumin and all plasminogenin serum samples■

No prior sample preparation needed■≥80% yield of stable antiserum free of albumin

and protease activity
For More Information
Web: bio-rad.com/affigelcm

CM Affi-Gel Blue Gel

Ordering Information
Catalog #Description

1537304
153-7305

CM Affi-Gel Blue Gel, 100 ml
CM Affi-Gel Blue Gel, 1 L

Price, U.S. $650.00Affi-Gel Boronate Resin
Affi-Gel boronate-derivatized polyacrylamide resin has
affinity for coplanar adjacent cis-hydroxyl groups (cis-diols)
and a high binding capacity, which provides highly efficient
separation of low MW molecules such as nucleotides,
nucleosides, catecholamines, and sugars. It has a sorbitol
capacity of 130 µmol/ml.
For More Information
Web: bio-rad.com/affigelboronate
Download bulletin: 1066

Affi-Gel Boronate Gel

Ordering Information
Catalog #Description

15361031536104

Affi-Gel Boronate Gel, 5 g
Affi-Gel Boronate Gel, 50 g

114 | Chromatography: Laboratory and Process Separations

Price, U.S. $650.00InquireIn the U.S., call toll free at 1-800-424-6723

Chromatography Media
bio-rad.com/affinityresin

Affinity Chromatography

Affi-Prep Polymyxin Resin
Affi-Prep polymyxin resin consists of 2–4 mg of USP-grade
polymyxin per ml of the macroporous polymeric Affi-Prep
support. Affi-Prep polymyxin resin binds endotoxins froma number of different strains of gram-negative bacteria
including E. coli, Salmonella abortus, S. minnesota,
and Serratia marcescens. Features of Affi-Prep polymyxin
resin include:■

Endotoxin removal in research andprocess-scale applications■

Linear flow rates to 2,000 cm/hr■

Pressure stability to 1,000 psi (70 bar)■ igh chemical stability (withstands sanitization withH
0.1 M NaOH)

Affi-Prep Polymyxin Resin

For More Information
Web: bio-rad.com/affigelpolymyxin

Ordering Information
Catalog #Description

1560010Affi-Prep Polymyxin Resin, 25 ml

Price, U.S. $835.00Affi-Gel Heparin Resin
Affi-Gel heparin resin is a ready-to-use support for the
purification of a range of proteins such as coagulation
factors, other plasma proteins, polynucleotide
polymerases, nucleases, lipases, lipoproteins, andproteases. Heparin binds a variety of enzymes and otherproteins, either ionically or by other specific enzymeinhibitor (or enzyme-activator) interactions. Features ofAffi-Gel heparin resin include:■

Heparin content ≥0.6 mg/ml■

Human antithrombin III binding capacity ≥1.2 mg/ml■

Linear flow rates of 10–20 cm/hr

Affi-Gel Heparin Gel

For More Information
Web: bio-rad.com/affigelheparin

Ordering Information
Catalog #Description

1536173

Affi-Gel Heparin Gel, 40 ml

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $525.00Chromatography: Laboratory and Process Separations | 115

Chromatography Media
Affinity Chromatography

bio-rad.com/affinityresin

Affi-Gel 10 and Affi-Gel 15 Resins
Affi-Gel 10 and Affi-Gel 15 activated affinity resins provide
spontaneous, rapid, and highly efficient coupling of ligands
via primary amines. Affi-Gel 10 resin is most efficient forcoupling neutral or basic proteins with pI 6.5–11.
Affi-Gel 15 resin is recommended for coupling acidic
proteins with pI <6.5. Affi-Gel 10 and 15 resins offer:

■

■

■

Aqueous or anhydrous coupling conditions
Complete protein coupling within 4 hr at 4°C
Protein coupling capacity up to 35 mg/ml of resin

For More Information
Web: bio-rad.com/affigel10and15
Download bulletin: 1085

Ordering Information
Catalog #Description

1536099
1536046
153-1000
1536051
1536052
1536098

Affi-Gel 10 Media, 25 ml
Affi-Gel 10 Media, 4 x 25 ml
Affi-Gel 10 Media, 1 L
Affi-Gel 15 Media, 25 ml
Affi-Gel 15 Media, 4 x 25 ml
Affi-Gel 10/15 Combination, includes 2 x 25 ml Affi-Gel 10 media and 2 x 25 ml Affi-Gel 15 media

Price, U.S. $175.00545.00Inquire195.00615.00560.00Affi-Gel Hz Hydrazide Resin
Affi-Gel Hz hydrazide activated resin couples immunoglobulin
G (IgG) molecules via carbohydrate moieties on the Fc regionof antibody molecules. Fc attachment results in a more
specific antigen-antibody interaction and 100–300% higher
antigen binding capacity than other resins. Affi-Gel Hz resin
advantages include:

■

■

■

■

Stable covalent hydrazone bonds
Mild oxidation without alteration of antibody activity
High antigen binding capacity
pH stability

For More Information
Web: bio-rad.com/affigelhydrazide

Ordering Information

See Also
Affinity media
selection guide:
page 86.

Catalog #Description

1536047
153-6054

Affi-Gel Hz Hydrazide Media, 25 ml
Affi-Gel Hz 10x Coupling Buffer Concentrate, 500 ml

Price, U.S. $315.00150.00Carbodiimide Activated Resin
Affi-Gel 102 resin is for use with 1-ethyl-3(3-dimethylaminopropyl)carbodiimide hydrochloride(EDAC or EDC) carbodiimide coupling reagent, which
immobilizes ligands that contain primary or terminal
carboxyl groups. The resin offers flexible alternative
chemistries and economy.

Affi-Gel 102 amino-terminal cross-linked agarose
resins with a six-atom hydrophilic arm feature:■■

EDAC carbodiimide coupling reagent
Compatibility with carboxyl-containing ligands

For More Information
Web: bio-rad.com/carbodiimide

Ordering Information
Catalog #Description

1532401

Affi-Gel 102 Media, 50 ml

116 | Chromatography: Laboratory and Process Separations

Price, U.S. $305.00In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Size Exclusion Chromatography

bio-rad.com/sizeexclusion

Size Exclusion Chromatography
See Also

Bio-Gel P Resins

Sample preparation
products: page 1.

Bio-Gel P polyacrylamide, for high-resolution gel filtration,
prepared by copolymerization of acrylamide and N,N'methylene-bis-acrylamide. Bio-Gel P resin:■

Bio-Spin andMicro Bio-Spinprepacked columns:
page 134.

Is available in several particle size ranges with molecularweight exclusion limits ranging from 100–100,000■

Is extremely hydrophilic and essentially nonionic■Provides efficient, gentle gel filtration of sensitive compounds■

Gel filtration
chromatography
standard: page 122.

Empty Econo-Pac
columns: page 141.
ENrich SEC
columns: page 126.

 oes not support microbial growth or leachD
carbohydrates (due to its synthetic composition) as
dextrose and agarose gels can

Bio-Gel P Resin

Bio-Gel P resin is autoclavable at pH 5.5–6.5 and operates
over a pH range of 2–10 at room temperature. Flow rate
and resolution increase with increasing temperature in therange of 4– 80°C.
Bio-Gel P polyacrylamide resin is available as bottled resinor as prepacked spin columns, columns and cartridges.
Columns and cartridges are packed with Bio-Gel P-6 resin,
while spin columns come prepacked with both P-6 andP-30 resin in either Tris or SSC buffer.
For More Information
Web: bio-rad.com/biogelp
Download bulletin: 2068

Bio-Scale Mini Bio-Gel Cartridges

Bio-Gel P Polyacrylamide Resin Selection GuideMedia

MW Fractionation Range

Bio-Gel P-2
Bio-Gel P-4
Bio-Gel P-6
Bio-Gel P-6DG
Bio-Gel P-10
Bio-Gel P-30
Bio-Gel P-60
Bio-Gel P-100

100–1,800
800–4,000
1,000–6,000
1,000–6,000
1,500–20,000
2,500–40,000
3,000–60,000
5,000–100,000

Ordering Information
Catalog #Description

Comments

1504114150-4115
12002617
1504118
150412015041241504128
1504130
1504134
1504138
1500738150-0739
1504140
15041441504150
1504154
12002610
1504160
1504164
12002609
1504170
1504174

Bio-Gel P-2 Media, fine, 100 g
Bio-Gel P-2 Media, fine, 500 g
Bio-Gel P-2 Media, fine, 1 kg
Bio-Gel P-2 Media, extra fine, 100 g
Bio-Gel P-4 Media, medium, 100 g
Bio-Gel P-4 Media, fine, 100 g
Bio-Gel P-4 Media, extra fine, 100 g
Bio-Gel P-6 Media, medium, 100 g
Bio-Gel P-6 Media, fine, 100 g
Bio-Gel P-6 Media, extra fine, 100 g
Bio-Gel P-6DG Media, 100 g
Bio-Gel P-6DG Media, 1 kg
Bio-Gel P-10 Media, medium, 100 g
Bio-Gel P-10 Media, fine, 100 g
Bio-Gel P-30 Media, medium, 100 g
Bio-Gel P-30 Media, fine, 100 g
Bio-Gel P-30 Media, fine, 1 kg
Bio-Gel P-60 Media, medium, 100 g
Bio-Gel P-60 Media, fine, 100 g
Bio-Gel P-60 Media, fine, 1 kg
Bio-Gel P-100 Media, medium, 100 g
Bio-Gel P-100 Media, fine, 100 g

Rapid carbohydrate, peptide, and protein desalting

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Carbohydrate and peptide separations, protein desalting

Purification of proteins and polypeptides

Rapid carbohydrate, peptide, and protein desalting;also available in prepacked columns and cartridges
Purification of proteins and polypeptides

295.001,090.00
295.00295.00295.00330.00330.00260.00295.00295.00295.00Inquire330.00330.00330.00345.00345.00345.00345.00345.00330.00330.00continues

Chromatography: Laboratory and Process Separations | 117

Chromatography Media
Size Exclusion Chromatography

bio-rad.com/sizeexclusion

Ordering Information
Catalog #Description

Econo-Pac Bio-Gel P-6 Desalting Columns
7322010
Econo-Pac 10DG Desalting Columns, pack of 30

145.00

EconoFit Bio-Gel P-6 Desalting Columns
12009238
EconoFit Bio-Gel P-6 Desalting Column, 1 x 5 ml
12009239
EconoFit Bio-Gel P-6 Desalting Columns, 5 x 5 ml
Bio-Scale Mini Bio-Gel P-6 Desalting Cartridge
7325304
Bio-Gel P-6 Desalting Gel, 5 x 10 ml
7325312
Bio-Gel P-6 Desalting Gel, 1 x 50 ml
7325314
Bio-Gel P-6 Desalting Gel, 5 x 50 ml
6200238
Profinia Desalting Purification Kit, 50 ml, includes 2 desalting and cartridge cleaning buffer kits,
1 x 50 ml desalting cartridge
Adaptor Fittings
7885010732011173201127320113

Catalog #Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect maleend of luer column to NGC system
Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system
Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge toan HPLC system
Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings kit, includes 1/4–28
female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow andNGC systems 59.00
Description

Contents

MW

pl

For Use withGel Filtration
Standard, 6 vials

Thyroglobulin
Bovine g-globulin
Chicken ovalbumin
Equine myoglobin
Vitamin B12670,000
158,000
44,00017,000
1,350

4.5
5.1
4.6
6.9
4.5

ENrich SEC
columns;
Bio-Gel P media

145.00145.0097.00

Price, U.S. $Standard
1511901

235.00

Bio-Gel A 1.5m Resin
Bio-Gel A 1.5m resin, ideal for purification of antibodies andaggregates, consists of agarose beads in which the poresize is controlled by the percentage of agarose in the gel. It
is compatible with all commonly used buffers and can beused with high-salt buffers without significantly

changing the bed volume. Bio-Gel A 1.5m resin may
be used at pH 4–13 and at temperatures 2–30°C.
The fractionation range is 10,000–1,500,000 daltons.
For More Information
Web: bio-rad.com/biogela

Ordering Information
Catalog #Description

151-0450

Bio-Gel A 1.5m Gel, fine, 500 ml

Price, U.S. $Inquire118 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Hydrophobic Interaction Chromatography

bio-rad.com/HIC

See Also

Bio-Beads S-X Resins
Bio-Beads S-X resins are neutral porous styrene
divinylbenzene beads for size exclusion chromatographyof lipophilic polymers and other solutes that requireorganic eluents. MW exclusion limits range from
400–14,000. This range is useful for fractionation of low
MW organic polymers and other hydrophobic substances.
Exclusion limits are influenced by the eluent used.
Bio-Beads S-X resins require an eluent that is mobile;
therefore, the beads must be used in a column. The beads
are compatible with benzene, toluene, xylene, carbon
tetrachloride, dimethylformamide, ketones, aromatics,

methylene chloride, o-dichlorobenzene, perchloroethylene,
tetrahydrofuran, and trichlorobenzene.

ENrich SEC
columns: page 126.

Recommended flow rates depend upon the cross-linkage:■

1% cross-linked resins are used only with gravity flow■

3% cross-linked resins can withstand a flow of 5 ml/min

with a backpressure of 20 bar or 300 psi■

8% and 12% cross-linked resins can withstandbackpressure up to 33 bar or 500 psi
For More Information
Web: bio-rad.com/biobeads

Ordering Information
Catalog #Description

Size, µm ml/dry g* Application**

1522150
152-2151
1522750
152-2753
15233501523650

Bio-Beads S-X1 Media, 100 g
Bio-Beads S-X1 Media, 1 kg
Bio-Beads S-X3 Media, 100 g
Bio-Beads S-X3 Media, 1 kg
Bio-Beads S-X8 Media, 100 g
Bio-Beads S-X12 Media, 100 g

40–80
40–80
40–80
40–80
40–80
40–80

7.5
7.5
4.75
4.75
3.1
2.5

Price, U.S. $1% cross-linked; for lipophilic polymers of MW 600–14,000
1% cross-linked; for lipophilic polymers of MW 600–14,000
3% cross-linked; for organic compounds of MW ≤2,000
3% cross-linked; for organic compounds of MW ≤2,000
8% cross-linked; for organic compounds of MW ≤1,000
12% cross-linked; for organic compounds of MW ≤400

465.003 Inquire465.00465.00465.00540.00Larger volumes and special packaging for industrial applications are available on request.
* Swollen in benzene.
** MW range is for beads fully swollen in benzene.

Hydrophobic Interaction Chromatography
Macro-Prep HIC Resins
Macro-Prep HIC resins are methacrylate-based beads forprotein, polypeptide, enzyme, and nucleic acid purification.
They are autoclavable and can withstand treatment inacid, base (pH up to 10), chaotropic agents, or detergents
while retaining high protein binding capacities. They areavailable in two functional forms: a weakly hydrophobic
methyl support for purification of compounds with stronghydrophobic regions, and a mildly hydrophobic t-butyl
support for purification of compounds with few or weaklyhydrophobic regions. Both resins are chemically andthermally stable. They can be cleaned in place with ethanol
or 1% acetic acid and 1% phosphoric acid.

Macro-Prep Methyl HIC Support

For More Information
Web: bio-rad.com/macroprepHIC
Download bulletin: 1841

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 119

Chromatography Media
Hydrophobic Interaction Chromatography

bio-rad.com/resinsampling

Ordering Information
Description
Macro-Prep Methyl HIC Support, 25 ml
Macro-Prep Methyl HIC Support, 100 ml
Macro-Prep Methyl HIC Support, 500 ml
Macro-Prep Methyl HIC Support, 5 L
Macro-Prep Methyl HIC Support, 10 L
Macro-Prep t-Butyl HIC Support, 25 ml
Macro-Prep t-Butyl HIC Support, 100 ml
Macro-Prep t-Butyl HIC Support, 500 ml
Macro-Prep t-Butyl HIC Support, 10 L
Catalog #20% EtOH
Shipping Solution

2% BnOH
Shipping Solution

15800801560080
156-0081
156-0082
156-0083
15800901560090156-0091
156-0093

12003687
12003688
12003689
12003690
12003691
12003692
12003693
12003694
12003695

Description

Price, U.S. $105.00395.00InquirePrice, U.S. $EconoFit Macro-Prep HIC Columns*
12009277
EconoFit Macro-Prep Methyl Column, 1 x 1 ml, 7 mm x 25 mm
12009278
EconoFit Macro-Prep t-Butyl Column, 1 x 1 ml, 7 mm x 25 mm

465.00465.00Inquire* Maximum pressure 72 psi.
EtOH, ethanol; BnOH, benzyl alcohol.

InquireBio-Beads SM-2 Adsorbents
Bio-Beads SM-2 nonpolar polystyrene adsorbents areanalytical-grade neutral macroporous polymeric beads witha high surface area for adsorbing organics of MW <2,000.
These beads can be used in aqueous solution and withsolvents or solvent mixtures, including alcohols, petroleum
ether, diethyl ether, and hexane, without expansion orcontraction of the beads.
Common applications of Bio-Beads SM-2 adsorbents
include the removal of detergents such as Triton X-100, theremoval of organics such as polyaromatic hydrocarbons
from water, cleanup of drugs from plasma and urine,cleanup of biological metabolites and pesticides, andcleanup of dyes and mycotoxins from food products.

Bio-Beads SM-2 Adsorbent

For More Information
Web: bio-rad.com/biobeads_SM2
Download bulletin: 1461
Request or download bulletin: 6905

Ordering Information
Catalog #Description

15289201523920152-3922
152-3923

Bio-Beads SM-2 Adsorbents, 25 g
Bio-Beads SM-2 Adsorbents, 100 g
Bio-Beads SM-2 Adsorbents, 1 kg
Bio-Beads SM-2 Adsorbents, 10 kg

120 | Chromatography: Laboratory and Process Separations

Price, U.S. $465.00465.00InquireInquireIn the U.S., call toll free at 1-800-424-6723

Chromatography Media
bio-rad.com/resinsampling

Screening and Application Packs, and Standards

Screening and Application Packs, and Standards
See Also

EconoFit Screening and Application Packs

Ion exchange resins:
page 88.

Maximize method optimization and parameter screening
for biomolecule purification with these convenient, readyto-use application and resin screening packs. These
packs address the entire range of needs for biomolecule
purification from capture, intermediate contaminantremoval, and final polishing.
■■■

Macro-Prep ion
exchange media:
page 91.
UNOsphere andNuvia ion exchange
media: page 88.
CHT ceramichydroxyapatite:
page 101.

Convenient prepacked columns

CFT ceramic
fluoroapatite: page 104.

Ideal for screening and optimization of purification
protocols

Macro-Prep HIC
resins: page 119.

Reproducible and scalable results

For More Information
Web: bio-rad.com/econofit

Ordering Information
Catalog #Description

Price, U.S. $EconoFit Packs
17007881
EconoFit CHT Screening Pack, includes 1 ml column each of CHT Type I, 40 μm; CHT Type II, 40 μm; CHT XT and
CFT Type II, 40 μm
17007892
EconoFit AEX Screening Pack, includes 1 ml column each of Macro-Prep High Q, Macro-Prep High Q-3HT, Macro-Prep
DEAE, UNOsphere Q, Nuvia Q, and Nuvia HP-Q
17007872
EconoFit CEX Screening Pack, includes 1 ml column each of Macro-Prep High S, Macro-Prep CM, UNOsphere S,
Nuvia S and Nuvia HR-S
17007891
EconoFit mAb Purification Pack, includes 1 ml column each of Nuvia S, Nuvia Q, Nuvia cPrime, Nuvia aPrime 4A,
Nuvia HR-S, and CHT XT
17007893
EconoFit Large Biomolecule Purification Pack, includes 1 ml column each of Nuvia Q, Nuvia HP-Q, Nuvia cPrime,
Nuvia aPrime 4A, CHT II, 40 μm; and CHT XT
17007894
EconoFit Aggregate Clearance Pack, includes 1 ml column each of Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, CHT XT
17007882
EconoFit IMAC and Desalting Pack, includes 5 ml column each of Bio-Gel P-6 Desalting and Nuvia IMAC

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 121

Chromatography Media
Screening and Application Packs, and Standards

bio-rad.com/chromstandards

Chromatography Standards
Ion Exchange Chromatography Standards
Bio-Rad offers two protein standards for ion exchange
chromatography that are suitable for use with bulk resins,
cartridges, or columns. Each standard is supplied as aset of six vials of lyophilized protein mixture for qualitative
analysis only.
 rganic Acid StandardO
Bio-Rad’s organic acid standard is supplied as a set of six
vials of lyophilized mixture for qualitative analysis only.

C arbohydrate Analysis Standard
Bio-Rad’s carbohydrate standard is supplied as a setof six vials of lyophilized mixture for qualitative analysis
only. The standards can be used for column testing or
semiquantitative determination.

Gel Filtration Chromatography Standard
Bio-Rad’s gel filtration standard is a calibration standard
for size exclusion columns used in protein purification.
The mixture includes vitamin B12 and myoglobin, which arevisible when eluting from glass or clear plastic columns, toensure that the column is properly packed and the sample
is eluting evenly. The standard can be used with most size
exclusion HPLC columns. The standard is supplied as aset of six vials of lyophilized protein mixture.
For More Information
Web: bio-rad.com/chromstandards

Standard Specifications
Description

Contents

MW

pl

For Use withProtein Standard for Anion
Exchange Chromatography		Equine myoglobin
Conalbumin
Chicken ovalbumin
Soybean trypsin inhibitor

17,00077,00045,00021,500

6.9
4.9
4.6
4.5

ENrich S columns; UNO S columns;
EconoFit Macro-Prep High S,
Macro-Prep CM, UNOsphere S, andNuvia S columns; Macro-Prep
High S, Macro-Prep CM,
UNOsphere S, and Nuvia S resins

Protein Standard for Cation
Exchange Chromatography		Equine myoglobin
Ribonuclease A
Cytochrome c

17,00013,50012,000

6.9
8.7
10.7

ENrich Q columns; UNO Q columns;
EconoFit Macro-Prep High Q,
Macro-Prep DEAE, UNOsphere Q,
and Nuvia Q columns; Macro-Prep
High Q, Macro-Prep DEAE,
UNOsphere Q, and Nuvia Q resins

Organic Acid Analysis
Standard
		
		
		
		

Sodium oxalate
Sodium citrate
Sodium malate
Sodium succinate
Sodium formate
Sodium acetate

134		
294		
196		
270
69
82

Carbohydrate Analysis
Standard
		
		
		
		

Melezitose
MaltoseGlucoseMannoseFructose
Adonitol (ribitol)504		
Aminex HPX-87C column		
360		
180		
180
180
152

Gel Filtration Standard
		
		
		
		

Thyroglobulin
Bovine g-globulin
Chicken ovalbumin
Equine myoglobin
Vitamin B12670,000
158,000
44,00017,0001,350

122 | Chromatography: Laboratory and Process Separations

Aminex HPX-87H column

ENrich SEC columns; Bio-Gel P
4.5
resins; Bio-Gel A 1.5m resin
5.1
4.6				6.94.5In the U.S., call toll free at 1-800-424-6723

Chromatography Media
Screening and Application Packs, and Standards

bio-rad.com/chromstandards

Ordering Information
Catalog #Description

Contents

MW

pl

For Use with

1250561

Protein Standard
for Anion Exchange
Chromatography,
6 vials

Equine myoglobin
Conalbumin
Chicken ovalbumin
Soybean trypsin inhibitor

17,00077,00045,00021,500

6.9
4.9
4.6
4.5

ENrich Q columns; UNO Q columns;
EconoFit Macro-Prep High Q,
Macro-Prep DEAE, UNOsphere Q,
and Nuvia Q columns; Macro-Prep
High Q, Macro-Prep DEAE,
UNOsphere Q, and Nuvia Q resins

280.001250562

Protein Standard for Equine myoglobin
Cation Exchange
Ribonuclease A
Chromatography,
Cytochrome c
6 vials

17,00013,50012,000

6.9
8.7
10.7

ENrich S columns; UNO S columns;
EconoFit Macro-Prep High S,
Macro-Prep CM, UNOsphere S, andNuvia S columns; Macro-Prep High S,
Macro-Prep CM, UNOsphere S, andNuvia S resins

280.001250586

Organic Acid
Analysis Standard,
6 vials

Sodium oxalate
Sodium citrate
Sodium malate
Sodium succinate
Sodium formate
Sodium acetate

134
294
196
270
69
82

Aminex HPX-87H column

165.001250585

Carbohydrate
Analysis Standard,
6 vials

Melezitose
MaltoseGlucoseMannoseFructose
Adonitol (ribitol)

504
360
180
180
180
152

Aminex HPX-87H column

165.001511901

Gel Filtration
Standard, 6 vials

Thyroglobulin
Bovine g-globulin
Chicken ovalbumin
Equine myoglobin
Vitamin B12670,000
158,000
44,00017,0001,350

ENrich SEC columns; BioGel P resins; Bio-Gel A
1.5m resin

235.00In the U.S., call toll free at 1-800-424-6723

4.5
5.1
4.6
6.9
4.5

Price, U.S. $Chromatography: Laboratory and Process Separations | 123

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/columns

Chromatography Columns
A complete range of prepacked and empty columns and accessories is available for protein and peptide separations.
Bio-Rad’s many disposable spin and gravity chromatography columns are made of chemically compatible polypropylene,
are autoclavable, and can be washed with NaOH. Our low-pressure glass Econo-Column chromatography columns
offer an ideal combination of performance, reliability, and value. For high-resolution separations, Bio-Rad’s Bio-Scale MT
medium-pressure columns can be run on a variety of liquid chromatography systems. Econo Alpha columns are easy-topack, compatible with NGC systems and other chromatography systems, and suitable for a range of applications.
Learn More about the Technology
Web: bio-rad.com/tech/chrom

Prepacked Chromatography Columns
Prepacked Column Selection Guide by Application
Application

Media

Type of Separation

Chemical Form

Binding Capacity/ml

Antibody/mAb
Purification

UNOsphere SUPrA
Affi-Prep Protein A

AffinityAffinityrProtein A
Protein A

UNOsphere Q
Nuvia Q
Nuvia cPrime–N(CH3)3+–N(CH3)3+–N(CH3)3+
–SO3–
–SO3–

IgG: 110 mg/ml
IgG: ≥70 mg/ml

CHT Type I, 40 µm
CHT Type II, 40 µm
CHT XT
MPC
CFT
DEAE Affi-Gel Blue

Strong anion exchange
Strong anion exchange
Hydrophobic cationexchange
Strong cation exchange
High-resolution strong
cation exchange
Hydroxyapatite
Hydroxyapatite
Hydroxyapatite
Hydroxyfluoroapatite
Fluoroapatite
Affinity

Human IgG: 30 mg/ml*
Mouse monoclonal IgG: 8–10 mg;
human IgG: 16–23 mg
BSA: 180 mg/ml*
IgG: >170 mg/ml at 300 cm/hr
Lactoferrin: >60 mg/ml; IgG: 40 mg/ml

Ca5(PO4)3(OH)2
Ca5(PO4)3(OH)2
Ca5(PO4)3(OH)2
Ca10(PO4)6(OH)1.5(F)0.5
Ca10(PO4)6F2
Cibacron Blue F3GA
and DEAE

Human IgG: 25–60 mg
Human IgG: 15–25 mg
mAb G: >60 mg/ml
Human IgG: 25–50 mg/ml
Lysozyme: 14–21.5 mg/g
Serum: 0.2–1.0 ml

Affinity
Protein
Purification

Nuvia IMAC
Profinity IMAC
Profinity GST
Profinity eXact

AffinityAffinityAffinityAffinity

UNOsphere SUPrA

AffinityrProtein A

Human IgG: 30 mg/ml*

Protein
Purification

Nuvia Q
Nuvia HP-Q

Strong anion exchange
High-throughput strong
anion exchange
Hydrophobic cationexchange
Strong cation exchange
High-resolution strong
cation exchange
Strong cation exchange
Hydroxyapatite
Hydroxyapatite
Hydroxyfluoroapatite
Fluoroapatite–N(CH3)3+–N(CH3)3+

IgG: >170 mg/ml at 300 cm/hr
Thyroglobulin: ≥28 mg/ml at 150 cm/hr–N(CH3)3+

Lactoferrin: >60 mg/ml; IgG: 40 mg/ml

–SO3–
–SO3–

IgG: 110 mg/ml
IgG: ≥70 mg/ml–SO3–
Ca5(PO4)3(OH)2
Ca5(PO4)3(OH)2
Ca10(PO4)6(OH)1.5(F)0.5
Ca10(PO4)6F2

Human IgG: ≥49 mg
Human IgG: 25–60 mg/ml*
mAb G: >60 mg/ml
Human IgG: 25–50 mg/ml
Lysozyme: 14–21.5 mg/g

Nuvia S
Nuvia HR-S

Nuvia cPrime
Nuvia S
Nuvia HR-S
Macro-Prep High S
CHTCHT XT
MPC
CFT
High-Resolution ENrich Q
Polishing
ENrich S
ENrich SEC

Strong anion exchange

Nitrilotriacetic acid (NTA) Recombinant histidine-tagged protein: ≥40 mg
Iminodiacetic acid
Recombinant histidine-tagged protein: ≥15 mg
Glutathione
Recombinant GST-tagged protein: ≥10 mg/ml
Modified subtilisin
Tag-free maltose-binding protein/ml resin:>3 mg/ml–N(CH3)3+Albumin: 130 mg/ml

Strong cation exchange –SO3 –IgG: 120 mg/ml

Size exclusion

500–70,000 Da
5,000–650,000 Da–* Binding capacity based on bulk media; check individual instruction manuals for run conditions and specifications.

124 | Chromatography: Laboratory and Process Separations

continues

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/columns

Prepacked Column Selection Guide by Application
Application

Media

Type of Separation

Chemical Form

Binding Capacity/ml

Desalting, Buffer
Exchange

Bio-Gel P-6

Gel filtration

Polyacrylamide

CV: 10–22%

Purification ofSerum Proteins,
EnzymesAffi-Gel Blue

Affinity

Cibacron Blue F3GA

Serum: 0.2 ml;
albumin: 11 mg

Plasmid
Purification

Macro-Prep High Q
UNOsphere Q
UNOsphere S
Macro-Prep DEAE
CHTCHT XT
MPC
CFT

Strong anion exchange
Strong anion exchange
Strong cation exchange
Weak anion exchange
Hydroxyapatite
Hydroxyapatite
Hydroxyfluoroapatite
Fluoroapatite–N(CH3)3+–N(CH3)3+–SO3––HN+(C2H5)2Ca5(PO4 )3(OH)2
Ca5(PO4 )3(OH)2
Ca10(PO4 )6(OH)1.5(F)0.5
Ca10(PO4 )6F2

BSA: ≥37 mg
BSA: 180 mg
Human IgG: 60 mg
Protein: >35 mg
Human IgG: 25–60 mg/ml*
mAb G: >60 mg/ml
Human IgG: 25–50 mg/ml
Lysozyme: 14–21.5 mg/g

* Binding capacity based on bulk media; check individual instruction manuals for run conditions and specifications.		
See Also

ENrich High-Resolution Ion Exchange Columns

Ion exchange
chromatography
standards: page 122.

ENrich ion exchange columns are designed for highresolution separations at fast flow rates. ENrich columns
run at low backpressures on the NGC chromatographysystem or any other medium- to high-pressure purification
system. This allows more flow rate flexibility in both
sample viscosities and temperature conditions. The 1 ml
ENrich column can complete a high-resolution separationin about 10 minutes. ENrich columns are also available in
8 ml sizes.
The unique polymeric resin features:■High capacity even at high flow rates■

Superior quality, reproducible separations■

Lower backpressure than similar resins■

Stability from pH 2–12

ENrich glass columns — provide high-resolution
separation of biomolecules. The durable borosilicate
glass tube and PEEK 10-32 female end fittings arecompatible with all aqueous solvents.

 vailable for strong anion (Q) and strong cation (S)Aexchange

For More Information
Visit bio-rad.com/ENrich■

Ordering Information

Catalog #Description

ENrich High-Resolution Ion Exchange Columns
7800001
ENrich Q 5 x 50 Column
7800003
ENrich Q 10 x 100 Column
7800021
ENrich S 5 x 50 Column
7800023
ENrich S 10 x 100 Column
Catalog #Fittings
7500564
7500568
7800008
7800091
7800093

Column Recommended Column Flow Maximum Operating Pressure
Volume, Maximum Protein Dimensions
psi
MPa
bar
Price, U.S. $Load, mg/column (W x L), mm
ml
1
8
1
8

100
800
100
800

5 x 5010 x 100
5 x 5010 x 100

500
500
500
500

3.45
3.45
3.45
3.45

34.5
34.5
34.5
34.5

Description

Price, U.S. $Fitting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic
DuoFlow and NGC systems
UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use
with BioLogic DuoFlow and NGC systems
Fittings, 10-32 PEEK, pkg of 2, black
ENrich 5 Frit Kit, includes 2 frits, 1 frit remover, 2 O-rings
ENrich 10 Frit Kit, includes 2 frits, 1 frit remover, 2 O-rings

In the U.S., call toll free at 1-800-424-6723

1,620.00
4,775.00
1,620.00
4,775.00

60.00
47.00
20.00
InquireInquireChromatography: Laboratory and Process Separations | 125

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/columns

ENrich High-Resolution Size Exclusion Columns
ENrich size exclusion columns are designed for highresolution at fast flow rates. ENrich columns run at low
backpressures on the NGC chromatography system or
any other medium- to high-pressure purification system.
This allows more flow rate flexibility in both sample
viscosities and temperature conditions. The 24 ml ENrich
column can complete a high-resolution separation in lessthan 30 minutes at a flow rate of 1 ml/min.
The unique polymeric resin features:■High resolution based on size■

Superior quality, reproducible separations■

Lower backpressure than similar resins■

Stability from pH 2–12■

 eparation ranges from 500–70,000 andS
5,000–650,000 Da

ENrich glass columns — provide high-resolution
separation of biomolecules. The durable borosilicate glass
tube and PEEK 10-32 female end fittings are compatible
with all aqueous solvents.
For More Information
Web: bio-rad.com/ENrich

Ordering Information

Catalog #ColumnSeparation
ColumnMaximum Operating Pressure
Volume, Range Globular Dimensions(W x L), mm
psi
MPa
bar
Price, U.S. $Proteins, Da
ml

Description

ENrich High-Resolution Size Exclusion Columns
7801070
ENrich SEC 70 10 x 300 Column
7801650
ENrich SEC 650 10 x 300 Column
Catalog #Fittings
750056424
24

500–70,000
5,000–650,000

10 x 300
10 x 300

600
600

4.1
4.1

Description

1,755.00
1,755.00
Price, U.S. $7800008
7800093 itting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic
F
DuoFlow and NGC systems
UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use
with BioLogic DuoFlow and NGC systems
Fittings, 10-32 PEEK, pkg of 2, black
ENrich 10 Frit Kit, includes 2 frits, 1 frit remover, 2 O-rings

Catalog #Description

Contents

MW

pl

For Use withGel Filtration
Standard, 6 vials

Thyroglobulin
Bovine g-globulin
Chicken ovalbumin
Equine myoglobin
Vitamin B12670,000
158,000
44,00017,000
1,350

4.5
5.1
4.6
6.9
4.5

ENrich SEC columns;
Bio-Gel P resins; Bio-Gel
A 1.5m resin

7500568

41
41

60.00
47.00
20.00
InquirePrice, U.S. $Standard
1511901

126 | Chromatography: Laboratory and Process Separations

235.00In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
bio-rad.com/econofit

Prepacked Chromatography Columns

EconoFit Chromatography Columns
EconoFit chromatography columns are prepacked andeasy to use with a range of Bio-Rad’s chromatography
resins, offering scientists convenience and reliability
from lab scale through process development. The
columns’ flexible formats, high pressure rating, and easy
connection allow researchers to easily get started with
their purification.
■■■

 vailable in a variety of different resin formats for a range ofA
purification needs
 tandard 10-32 fittings for easy connection to manyS
different chromatography instruments
 igh pressure rating to meet different experimental needsH(72 psi)*■Prepacked and ready-to-use formats designed to save time■

Empty columns also available to pack resin of choice

For use with NGC systems or any chromatography system.
* The pressure tolerance of the column hardware is 72 psi. The
pressure tolerance of some packed resins is lower than 72 psi.
For More Information
Web: bio-rad.com/econofit

Ordering Information
Catalog #Description

12009234
12009235
12009237

EconoFit Affi-Gel Blue Column, 1 x 5 ml, prepacked affinity column, 16 x 25 mm, maximum pressure 10 psi
EconoFit Affi-Gel Blue Columns, 5 x 5 ml, prepacked affinity columns, 16 x 25 mm, maximum pressure 10 psi
EconoFit Affi-Prep Protein A Column, 1 x 5 ml, prepacked affinity column, 16 x 25 mm,
maximum pressure 72 psi
EconoFit Affi-Prep Protein A Columns, 5 x 1 ml, prepacked affinity columns, 7 x 25 mm,
maximum pressure 72 psi
EconoFit Bio-Gel P-6 Desalting Column, 1 x 5 ml, 6 mm x 25 mm, maximum pressure 72 psi. For desalting or
buffer exchange
EconoFit Bio-Gel P-6 Desalting Columns, 5 x 5 ml, 16 x 25 mm, maximum pressure 72 psi. For desalting or
buffer exchange
EconoFit CFT Type II, 40 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
EconoFit CFT Type II, 40 µm Column, 1 x 5 ml, prepacked mixed-mode column, 16 x 25 mm,
maximum pressure 72 psi
EconoFit CFT Type II, 40 µm Columns, 5 x 5 ml, prepacked mixed-columns, 7 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type I, 40 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type I, 40 µm Column, 1 x 5 ml, prepacked mixed-mode column, 16 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type I, 40 µm Columns, 5 x 5 ml, prepacked mixed-mode columns, 16 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type I, 80 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type II, 40 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type II, 40 µm Column, 1 x 5 ml, prepacked mixed-mode column, 16 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type II, 40 µm Columns, 5 x 5 ml, prepacked mixed-mode columns, 16 x 25 mm,
maximum pressure 72 psi
EconoFit CHT Type II, 80 µm Column, 1 x 1 ml, prepacked mixed-mode columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit CHT XT Column, 1 x 1 ml, prepacked mixed-mode column, 7 mm x 25 mm, maximum pressure 72 psi
EconoFit DEAE Affi-Gel Blue Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm,
maximum pressure 10 psi
EconoFit DEAE Affi-Gel Blue Columns, 5 x 5 ml, prepacked affinity columns, 16 mm x 25 mm,
maximum pressure 10 psi

12009236
12009238
12009239
12009252
12009240
12009251
12009255
12009253
12009254
12009256
12009259
12009257
12009258
12009260
12009261
12009262
12009263

Price, U.S. $275.00660.00335.00450.00175.00145.0097.00
59.00

275.00660.00335.00450.00175.00continues

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 127

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/econofit

Ordering Information
Catalog #Description

12009273

EconoFit Macro-Prep CM Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep DEAE Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep DEAE Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep DEAE Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep DEAE Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High Q Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High Q Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High Q Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High Q-3HT Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High S Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High S Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep High S Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Macro-Prep Methyl HIC Column, 1 x 1 ml, prepacked hydrophobic interaction column,
7 mm x 25 mm, maximum pressure 72 psi
EconoFit Macro-Prep t-Butyl HIC Column, 1 x 1 ml, prepacked hydrophobic interaction column,
7 mm x 25 mm, maximum pressure 72 psi
EconoFit MPC I-40 Column, 1 x 1 ml, prepacked mixed-mode column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Nuvia aPrime 4A Column, 1 x 1 ml, prepacked hydrophobic anion exchange column,
7 mm x 25 mm, maximum pressure 45 psi
EconoFit Nuvia cPrime Column, 1 x 1 ml, prepacked hydrophobic cation exchange column,
7 mm x 25 mm, maximum pressure 45 psi
EconoFit Nuvia HP-Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia HR-S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia IMAC Column, 1 x 1 ml, prepacked Ni-charged column, 7 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia IMAC Column, 1 x 5 ml, prepacked Ni-charged column, 16 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia IMAC Columns, 5 x 1 ml, 1 ml, prepacked Ni-charged columns, 7 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia IMAC Columns, 5 x 5 ml, prepacked Ni-charged columns, 16 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia IMAC Uncharged Column, 1 x 1 ml, prepacked uncharged IMAC column,
7 mm x 25 mm, maximum pressure 45 psi
EconoFit Nuvia Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 45 psi
EconoFit Nuvia S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 45 psi
EconoFit Profinity eXact Column, 1 x 1 ml, prepacked affinity column, 7 mm x 25 mm,
maximum pressure 72 psi

12009274
12009265
12009264
12009266
12009275
12009268
12009267
12009269
12009283
12009276
12009271
12009270
12009272
12009277
12009278
12009279
12009280
12009281
12009282
12009284
12009288
12009286
12009285
12009287
12009289
12009290
12009291
12009292

128 | Chromatography: Laboratory and Process Separations

Price, U.S. $275.00660.00335.00450.00175.00145.00continues

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
bio-rad.com/econofit

Prepacked Chromatography Columns

Ordering Information
Catalog #Description

12009294

EconoFit Profinity eXact Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Profinity eXact Columns, 5 x 1 ml, prepacked affinity columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Profinity GST Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm,
maximum pressure 43 psi
EconoFit Profinity GST Columns, 5 x 1 ml, prepacked affinity columns, 7 mm x 25 mm,
maximum pressure 43 psi
EconoFit Profinity GST Columns, 5 x 5 ml, prepacked affinity columns, 16 mm x 25 mm,
maximum pressure 43 psi
EconoFit Profinity IMAC Column, 1 x 5 ml, prepacked Ni-charged column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit Profinity IMAC Columns, 5 x 1 ml, prepacked Ni-charged column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit Profinity IMAC Columns, 5 x 5 ml, prepacked Ni-charged columns, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere Q Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere Q Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere Q Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere S Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere S Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere S Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere SUPrA Column, 1 x 1 ml, prepacked affinity column, 7 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere SUPrA Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm,
maximum pressure 72 psi
EconoFit UNOsphere SUPrA Columns, 5 x 1 ml, prepacked affinity columns, 7 mm x 25 mm,
maximum pressure 72 psi
Empty EconoFit Columns, 5 x 1 ml, 7 mm x 25 mm, maximum pressure 72 psi
Empty EconoFit Columns, 5 x 5 ml, 16 mm x 25 mm, maximum pressure 72 psi

12009293
12009296
12009295
12009297
12009299
12009298
12009300
12009307
12009302
12009301
12009303
12009308
12009305
12009304
12009306
12009321
12009323
12009322
12009232
12009233

Price, U.S. $EconoFit Screening and Application Packs
17007881
EconoFit CHT Screening Pack, includes 1 ml column each of CHT Type I, 40 μm; CHT Type II, 40 mm;
CHT XT; and CFT Type II, 40 mm
17007892
EconoFit AEX Screening Pack, includes 1 ml column each of Macro-Prep High Q, Macro-Prep
High Q-3HT, Macro-Prep DEAE, UNOsphere Q, Nuvia Q, and Nuvia HP-Q
17007872
EconoFit CEX Screening Pack, includes 1 ml column each of Macro-Prep High S, Macro-Prep CM,
UNOsphere S, Nuvia S, and Nuvia HR-S
17007891
EconoFit mAb Purification Pack, includes 1 ml column each of Nuvia S, Nuvia Q, Nuvia cPrime,
Nuvia aPrime 4A, Nuvia HR-S, and CHT XT
17007893
EconoFit Large Biomolecule Purification Pack, includes 1 ml column each of Nuvia Q, Nuvia HP-Q,
Nuvia cPrime, Nuvia aPrime 4A, CHT II, 40 μm, and CHT XT
17007894
EconoFit Aggregate Clearance Pack, includes 1 ml column each of Nuvia cPrime, Nuvia aPrime 4A,
Nuvia HR-S, CHT XT
17007882
EconoFit IMAC and Desalting Pack, includes 5 ml column each of Bio-Gel P-6 Desalting and Nuvia IMAC

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 129

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/foresightForesight Prepacked Screening Tools: Plates and Columns
Foresight plates and columns are prepacked with a rangeof Bio-Rad’s process chromatography resins, offering
process scientists convenience and reliability for theirhigh-throughput experimentation needs. The products’
robust design allows researchers to use the prepacked
formats through the entire purification development cycle,
from high-throughput resin screening to small-scale
method development and scale-up optimization.
Benefits include:
■

■

■

■

 repacked and ready-to-use formats designed toPsave process development time bility to evaluate different experimental conditions toAbetter define an operational window■

Compatible with robotic liquid handling workstations
 tandard 10-32 male end fittings for easy connection ofS
column to many chromatography systems

 bility to perform high-throughput experiments withA
minimum sample requirements■

 vailable in a variety of chromatography modes that areA
designed for large-scale bioprocess

For More Information
Web: bio-rad.com/foresightDownload bulletin: 6297

Ordering Information
Catalog #Description

Foresight Plates*732-4714
Foresight UNOsphere Q, 20 µl
732-4710
Foresight UNOsphere S, 20 µl
732-4703
Foresight Nuvia Q, 20 µl
732-4701
Foresight Nuvia S, 20 µl
732-4707
Foresight Nuvia HR-S, 20 µl
12006908
Foresight Nuvia HP-Q, 20 μl
12007411
Foresight Nuvia aPrime 4A, 20 µl
732-4705
Foresight Nuvia cPrime, 20 µl
732-4716
Foresight CHT Type I, 40 µm 20 µl
732-4718
Foresight CHT Type II, 40 µm, 20 µl
12003151
Foresight CHT XT, 40 µm, 20 µl
732-4785
Foresight MPC Type I, 40 µm, 20 µl
12004035
Foresight Nuvia IMAC Ni-Charged, 20 µl

Price, U.S. $551.25551.25551.25551.25540.75551.25551.25551.25551.25continues

130 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/foresight

See Also

Ordering Information
Description
Foresight Columns
Foresight UNOsphere Q
Foresight UNOsphere S
Foresight Nuvia Q
Foresight Nuvia S
Foresight Nuvia HR-S
Foresight Nuvia HP-Q
Foresight Nuvia aPrime 4A
Foresight Nuvia cPrime
Foresight CHT Type I, 40 µm
Foresight CHT Type II, 40 µm
Foresight CHT XT, 40 µm
Foresight MPC Type I, 40 µm
Foresight Nuvia IMAC Ni-Charged
Description
Foresight RoboColumn Units**,***
Foresight UNOsphere Q
Foresight UNOsphere S
Foresight Nuvia Q
Foresight Nuvia S
Foresight Nuvia HR-S
Foresight Nuvia HP-Q
Foresight Nuvia aPrime 4A
Foresight Nuvia cPrime
Foresight CHT Type I, 40 µm
Foresight CHT Type II, 40 µm
Foresight CHT XT, 40 µm
Foresight MPC Type I, 40 µm
Foresight Nuvia IMAC Ni-Charged
Catalog#
Fittings
76013117500564

1 ml

5 ml

732-4732
732-4730
732-4721
732-4720
732-4723
12007020
12007392
732-4722
732-4735
732-4736
12003150
732-4737
12004038

732-4752
732-4750
732-4741
732-4740
732-4743
12007021
12007393
732-4742
732-4755
732-4756
12003149
732-4757
12004037

200 µl600 µl732-4819
732-4813
732-4804
732-4801
732-4831
12007013
12007394
732-4807
732-4822732-4825
12003152732-4828
12004051

732-4820
732-4814
732-4805
732-4802732-4832
12007014
12007395
732-4808
732-4823
732-4826
12003148
732-4829
12004052

Ion exchange
chromatography
standards: page 122.

Description
Long Fingertight Fittings, 10-32 x 1.03", for connecting 1/16" PEEK and Tefzel tubing to an FPLC system, 4Fitting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic
DuoFlow and NGC systems* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
*** Foresight RoboColumn units are to be used with robotic liquid handling workstations.
Visit bio-rad.com/foresight for more information on prepacked columns and plates.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 131

Process-Scale Separations
Process-Scale Prepacked Columns

bio-rad.com/ForesightProColumn

New Process-Scale Prepacked Chromatography Columns — GMP Ready

Foresight Pro Columns

Foresight Pro Chromatography Columns,
CHT Ceramic Hydoxyapatite
Bio-Rad offers Foresight Pro columns prepacked withCHT ceramic hydroxyapatite media. These are validated,
GMP ready columns specifically designed to be used forall biopharmaceutical manufacturing applications. The
columns have a sanitary design with minimal dead spaceand effective cleanability, and connections to pumping
systems are made via standard connections. CHT
prepacked columns are available in a range of column
diameters and bed heights. CHT prepacked columns areshipped in 0.1 N NaOH.
Foresight Pro column dimensions available with CHT
ceramic hydroxyapatite Type I, CHT Type II, and CHT XT
media (40 μm particle size).
Vessel Type Size
5
5
8
8
10
10
13
13
20
20
24
24
33
33
ID, inner diameter.

Tubes/Racks

Capacity

10
20
10
20
10
20
10
20
10
20
10
20
10
20

0.2
0.4
0.5
1
0.8
1.5
1.3
2.7
3.1
6.3
4.5
9
8.5
17

132 | Chromatography: Laboratory and Process Separations

■

■

■

■

■

■

■

■

■

GMP ready columns — validated and ready to use xcellent performance — data include asymmetry, HETP,
E
endotoxin, and bioburden
Robust column design ensures quality packing
 olumns maintain performance criteria after shippingCand cycling
Validated through ISTA 2A transportation testing ll wetted parts meet USP Class VI requirements
Aof USP <88>
Standard Tri-Clamp connections — plug and play olumns are packed in a controlled ISO Class 7
Ccleanroom ange of column diameters and bed heights —R
5–33 cm ID and 10 or 20 cm bed height■

Resin reference sample included with each column■

Regulatory support file available

For More Information
Web: bio-rad.com/ForesightProColumn
User guide: bio-rad.com/ForesightProGuide

In the U.S., call toll free at 1-800-424-6723

Process-Scale Separations
bio-rad.com/ForesightProColumn

Process-Scale Prepacked Columns

Ordering Information
Catalog #Description
Foresight Pro Column, CHT Type I, 40 μm
12014659
Foresight Pro Column, CHT Type I, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014660
Foresight Pro Column, CHT Type I, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014671
Foresight Pro Column, CHT Type I, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014672
Foresight Pro Column, CHT Type I, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014673
Foresight Pro Column, CHT Type I, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014674
Foresight Pro Column, CHT Type I, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014675
Foresight Pro Column, CHT Type I, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014676
Foresight Pro Column, CHT Type I, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014677
Foresight Pro Column, CHT Type I, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014678
Foresight Pro Column, CHT Type I, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014679
Foresight Pro Column, CHT Type I, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014680
Foresight Pro Column, CHT Type I, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014681
Foresight Pro Column, CHT Type I, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014682
Foresight Pro Column, CHT Type I, 40 μm, 17 L, 33 cm ID x 20 cm length
Foresight Pro Column, CHT Type II, 40 μm
12014697
Foresight Pro Column, CHT Type II, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014698
Foresight Pro Column, CHT Type II, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014699
Foresight Pro Column, CHT Type II, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014700
Foresight Pro Column, CHT Type II, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014701
Foresight Pro Column, CHT Type II, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014702
Foresight Pro Column, CHT Type II, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014703
Foresight Pro Column, CHT Type II, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014704
Foresight Pro Column, CHT Type II, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014705
Foresight Pro Column, CHT Type II, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014706
Foresight Pro Column, CHT Type II, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014707
Foresight Pro Column, CHT Type II, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014708
Foresight Pro Column, CHT Type II, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014709
Foresight Pro Column, CHT Type II, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014710
Foresight Pro Column, CHT Type II, 40 μm, 17 L, 33 cm ID x 20 cm length
Foresight Pro Column, CHT XT, 40 μm
12014725
Foresight Pro Column, CHT XT, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014726
Foresight Pro Column, CHT XT, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014727
Foresight Pro Column, CHT XT, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014728
Foresight Pro Column, CHT XT, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014729
Foresight Pro Column, CHT XT, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014730
Foresight Pro Column, CHT XT, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014731
Foresight Pro Column, CHT XT, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014732
Foresight Pro Column, CHT XT, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014733
Foresight Pro Column, CHT XT, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014734
Foresight Pro Column, CHT XT, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014735
Foresight Pro Column, CHT XT, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014736
Foresight Pro Column, CHT XT, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014737
Foresight Pro Column, CHT XT, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014738
Foresight Pro Column, CHT XT, 40 μm, 17 L, 33 cm ID x 20 cm length

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 133

Chromatography Columns
Prepacked Chromatography Columns

See Also
Nucleic acid sample
preparation: page 9.

bio-rad.com/biospin

Bio-Spin and Micro Bio-Spin Columns
Bio-Spin P-6 and Micro Bio-Spin P-6 columns are idealfor desalting protein samples quickly using size exclusion
chromatography. Filled with Bio-Gel P-6 or P-30 resin,these columns are shipped fully hydrated in Tris buffer
and are ready to use. These products effectively clean up
and remove salts, nucleotides, dye terminators, and small
molecules from protein, RNA, and DNA samples in just
10 minutes. The columns are autoclavable.
■

■

■

■

End cap

Reservoir

Reservoir
3 cm
2 cm working bed height

5 cm

0.8 ml bed
volume3.7 cm working bed height
1.2 ml bed volume

 rovide fast salt and contaminant removal in anPeasy-to-use spin-column format

Porous 30 µm
polyethylene bed
support retains
fine particles

 emove compounds <6 kD by size exclusionR
chromatography

Micro Bio-SpincolumnLuer end
fitting withsnap-off tip

Luer end fittingwith snap-off tip

Accommodate up to 100 µl of sample (Bio-Spin columns)
 ccommodate up to 70 µl of sample
A(Micro Bio-Spin columns)

End cap

Bio-Spin column

For More Information
Web: bio-rad.com/biospin

Bio-Spin Column Selection Guide					

Bio-Spin P-6

Micro Bio-Spin P-6

Equilibration buffer

SSC buffer*

10 mM Tris, pH 7.4, or SSC buffer* SSC buffer*

Bio-Spin P-30

Micro Bio-Spin P-30
10 mM Tris, pH 7.4, or SSC buffer*

Applications
Desalting and buffer
Desalting and buffer					
exchangeexchange							

Desalting; nucleotide
and small molecule
removalRetention andrecovery

90% recovery of 20 bases
or bp, 99% retention of salts

90% recovery of 20 bases
or bp, 99% retention of salts

95% recovery of 22 bases or 95% recovery of 22 bases or
bp, 98% retention of ddNTPs bp, 98% retention of ddNTPs

MW exclusion limit,
globular proteins

6,000

6,000

40,00040,000Sample volume

50–100 µl

10–75 µl50–100 µl

10–75 µl

Centrifuge type

Swinging bucket

Microcentrifuge

Swinging bucket

Microcentrifuge

DNA sequencing reaction
mixtures (Tris) and small
molecule removal* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0.

Ordering Information
Catalog #Description

7326227
7326228
732622173262227326006
7326202

Bio-Spin P-6 Columns, 25 with Tris buffer
Bio-Spin P-6 Columns, 100 with Tris buffer
Micro Bio-Spin P-6 Columns, 25 with Tris buffer
Micro Bio-Spin P-6 Columns, 100 with Tris buffer
Bio-Spin P-30 Columns, 25 with SSC buffer
Micro Bio-Spin P-30 Columns, 25 with SSC buffer

Price, U.S. $135.00455.00110.00365.00155.00110.00

Go to bio-rad.com/cartridges for current information on prepacked cartridges.

134 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/columns

See Also

Mini Bio-Spin Columns

ProteoMiner protein
enrichment system:
page 7.

Mini Bio-Spin columns are available with 0.6 ml prepacked
resin for both affinity-tagged purification and protein
enrichment applications. The columns contain Profinity
eXact resins for affinity-tagged purification and on-column
cleavage; see page 110.
For More Information
Web: bio-rad.com/minibiospin
Download bulletins: Profinity eXact fusion-tag system — 5725, 5742,
and 5766; ProteoMiner beads — 3096 and 5635

Ordering Information
Catalog #Description

1563007*

Profinity eXact Mini Spin Columns, includes ten 0.6 ml spin columns, ten 2 ml capped tubes, and ten
2 ml capless tubes

Price, U.S. $165.00* For ProteoMiner kits, see page 7.

Poly-Prep Ion Exchange Columns
Poly-Prep prepacked columns for gravity-flow
chromatography provide convenience for sample
preparation and other small-scale applications.
The graduated polypropylene columns hold a standard
bed volume of 2 ml of AG ion exchange resin and include
an integral 10 ml reservoir. A Poly-Prep stack cap, whichallows connection to pumps, reservoirs,
or columns in series, is also available.
For More Information
Web: bio-rad.com/polyprep

End cap

10 ml ­reservoir
9 cm
2 ml bed v­ olume
Graduatedvolume ­markings
Porous 30 µm
polyethylene
bed s­ upport retains
fine ­particles

Luer end fittingwith snap-off tip

Ordering Information
Particle Ionic
Size, µm Form

Catalog #Description

7316211

Poly-Prep Columns, AG 1-X8
106–180
resin, 100–200 mesh, 50
Poly-Prep Columns, AG 1-X8
45–106
resin, 200–400 mesh, 50
Poly-Prep Columns, AG 1-X8
45–106
resin, 200–400 mesh, 50
Poly-Prep Columns, AG 50W-X8, 106–250
100–200 mesh, 50
Poly-Prep Columns, AG 50W-X8, 63–150
200–400 mesh, 50
Poly-Prep Column Stack Cap, 50
Stopcock, Lock 2-Way Luer, 10731621273162217316213731621473115557328102

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Application

Chloride

Separation of low molecular weight inorganic anions

245.00Chloride

For high-resolution random separations

245.00FormateSeparation of low molecular weight biological compounds
such as nucleotides, peptides, and carboxylic acids
Hydrogen Separation and concentration of low molecular weightcations such as small peptides and amino acids
Hydrogen For high-resolution random separations

245.00250.00Allows connection of pumps, reservoirs, or columns in series
Provides control of flow-through Poly-Prep columns

43.00
55.00

245.00Chromatography: Laboratory and Process Separations | 135

Chromatography Columns
Prepacked Chromatography Columns

See Also
NGC systems:
page 150.
CHT ceramichydroxyapatite:
page 101..

bio-rad.com/columns

Bio-Scale CHT Type I Columns
Bio-Scale CHT Type I columns are packed with CHT ceramichydroxyapatite, Type I 10 µm resin, which has high affinity
for basic proteins and lower affinity for acidic proteins.
These prepacked columns allow rapid, reproducible
high-resolution separations for analytical to semipreparative
medium-pressure applications. These columns are ideal for
use with any medium- to high-pressure chromatographysystem. Available bed volumes are 2, 5, 10, and 20 ml.
Bio-Scale CHT Type I Column Top-Off Support Kit
If the top of the column bed becomes fouled and the usual
hygiene steps do not restore performance, a few millilitersof the bed can be removed and replaced with fresh
support. The Bio-Scale CHT Type I column top-off support
kit contains 1 ml support, frits, and distribution screens foreach column diameter.
For More Information
Web: bio-rad.com/bioscaleCHT

Ordering Information

See Also
UNOsphere ion
exchange resin:
page 88.
NGC systems:
page 150.

Catalog #Description

75100217510023751002575100277510029

Bio-Scale CHT2-I Column
Bio-Scale CHT5-I Column
Bio-Scale CHT10-I Column
Bio-Scale CHT20-I Column
CHT-I Top-Off Support Kit,
1 ml

ColumnVolume, ml

Recommended
Flow Rate,
ml/min

2
5
10
20

20
50
100
200

Maximum
ColumnRecommended
OperatingDimensions
Flow Rate,(W x L), mm Pressure, psi Price, U.S. $ml/min0.5–3.0
0.5–5.0
0.5–7.0
0.5–10.0

7 x 5210 x 64
12 x 88
15 x 113

1,000
750
600
500

1,140.00
1,795.00
2,910.00
4,485.00
400.00

UNO Monolith Ion Exchange Columns
UNO monolith ion exchange columns contain a patented*
continuous-bed matrix, allowing biomolecule separations
at high flow rates without sacrificing resolution or capacity.
UNO columns run at low backpressures on medium- tohigh-pressure chromatography systems. A 1 ml UNO
column can complete a high-resolution separation inabout 3 minutes. UNO columns are also available in a
6 ml size.
The unique homogeneous UNO matrix:■■

 rovides large pore diameters especially suited forP
purifying larger proteins, DNA, and virus
Has high capacity, even at high flow rates, due to thedense network of nodules that contain the ionic functionalgroups. These groups are completely accessible tobiomolecules via the interconnecting channels■

Has the highest quality and batch-to-batch reproducibility■

Prevents fragmentation so columns last longer■

Is stable from pH 2–12■

Is available for strong anion (Q) and strong

cation (S) exchange* U.S. patent 6,423,666.

136 | Chromatography: Laboratory and Process Separations

UNO replacement columns — UNO replacement columns
provide a simple bed replacement as an alternative topurchasing a new column.
UNO glass columns — UNO glass columns provide highresolution separation of biomolecules. The transparent glass
tube allows easy bed inspection and column troubleshooting.
Three column sizes provide flexibility for purification protocols.
UNO polishing PEEK columns — UNO polishing columns,
0.16 ml, are late-stage purification tools to obtain the highest
resolution and recovery from small sample loads. They allow
you to purify and concentrate dilute samples in one step.
For More Information
Web: bio-rad.com/unomonolith

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/aminex

See Also

Ordering Information
Column Recommended Recommended Column Flow Maximum Operating
Pressure
Dimensions
Flow Rate,
Volume, Maximum Protein
Catalog #Descriptionml

Load, mg/column

ml/min(W x L), mm

psi

MPa

bar

Price, U.S. $7200001
720000372000217200023

UNO Q1 Column
UNO Q6 Column
UNO S1 Column
UNO S6 Column

1.3
6
1.3
6

20
90
20
90

0.5–5.0
0.5–8.0
0.5–5.0
0.5–8.0

7 x 3512 x 53
7 x 3512 x 53

700
700
700
700

4.5
4.5
4.5
4.5

48
48
48
48

1,760.00
3,835.00
1,760.00
3,835.00

UNO Replacement Columns
7200011
UNO Q1R Column
7200013
UNO Q6R Column
7200031
UNO SR1 Column
7200033
UNO S6R Column

1.3
6
1.3
6

20
90
20
90

0.5–5.0
0.5–8.0
0.5–5.0
0.5–8.0

7 x 3512 x 53
7 x 3512 x 53

700
700
700
700

4.5
4.5
4.5
4.5

48
48
48
48

965.002,835.00
965.002,705.00

UNO Polishing PEEK Columns
7200009
UNO Q Polishing Column
7200029
UNO S Polishing Column

0.16
0.16

2
2

0.1–1.0
0.1–1.0

4.6 x 10
4.6 x 10

200
200

1.3
1.3

14
14

610.00Catalog #Fittings
75005547500568
7500567

Description

Price, U.S. $1/16" OD (1.6 mm) Post-Pump Fittings, includes Delrin nut, ferrules, lock ring, 10 sets
UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use with
BioLogic DuoFlow and NGC systems
UNO M6 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an FPLC system

Aminex HPLC Columns
Aminex HPLC columns are packed with a polystyrene
divinylbenzene resin. Aminex resin has high pressurestability, wide pH stability, and high column efficiency andselectivity. Aminex HPLC columns separate compounds
using the ion-moderated partition chromatography
technique. Aminex columns are often used in the food,beverage, and biofuel industries. Aminex columns are
an industry standard for the analysis of carbohydrates,organic acids, organic bases, and other small organic
molecules, including peptides and nucleic acids.
To separate complex mixtures with Aminex columns,
simple isocratic mobile phases (often just water) and
precise temperature control are used.

Ion exchange
chromatography
standards: page 122.

185.0047.00
36.00

See Also
Organic acidstandard: page 122.
Carbohydrate
analysis standard:
page 122.
NGC systems:
page 150.

Aminex columns are commonly used for USP methods
under the "L" specification. Specific designations are listed
in the table below.
For More Information
Web: bio-rad.com/aminex
Download bulletins: 1928 and 6333

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 137

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/aminex

Ordering Information

Applications

Particle
Size,
µm

Ionic
Form

CrossLinkage,
%

pH
Range

USP
Number1250508

Beet sugars

9

Sodium

8

5–9

L58

1,430.00

1250128

Molasses,corn syrup
High fructose
corn syrup
Sugar alcohols

9

Potassium

8

5–9

—

1,430.00

9

Calcium

8

5–9

L19

1,395.00

9

Calcium

8

5–9

L19

1,045.00

1250119 or Pentose sugars,
1250118
cellulosehydrolysates,
biofuels

9

Lead

8

5–9

L34

1,565.00

9

Hydrogen

8

1–3

L17

1,430.00

25

Calcium

4

5–9—

1,360.00

25

Silver4

6–8—

1,805.00

1250119 or Glucose,
1250118
galactose,
sucrose, fructose

9

Lead

8

5–9

—

1,240.00

Organic Acid and Alcohol Columns
1250140
Aminex HPX-87H
Column, 300 x 7.8 mm

1250129

9

Hydrogen

8

1–3

L17

1,430.00

1250100

Fast Acid Analysis
Column, 100 x 7.8 mm

1250129

Sugars withorganic acids,
fermentation
monitoring,biofuels
Alcohol,glycol organicacid analysis

9

Hydrogen

8

1–3

—

1,135.00

1250115Fermentation
Monitoring Column,
150 x 7.8 mm

1250129

Sugar, acids,
alcohols

9

Hydrogen

8

1–3

—

1,295.00

Catalog #Description

Catalog #Description

Carbohydrate Analysis Columns
1250143
Aminex HPX-87N
Column, 300 x 7.8 mm
1250142
Aminex HPX-87K
Column, 300 x 7.8 mm
1250095
Aminex HPX-87C
Column, 300 x 7.8 mm
1250094
Aminex HPX-87C
Column, 250 x 4.0 mm
1250098
Aminex HPX-87P
Column, 300 x 7.8 mm

Guard
Column*1250128
1250128

1250140

Aminex HPX-87H
Column, 300 x 7.8 mm

1250129

1250096

Aminex HPX-42C
Column, 300 x 7.8 mm
Aminex HPX-42A
Column, 300 x 7.8 mm

1250128

1250097
1250105

Fast Carbohydrate
Column, 300 x 7.8 mm

1250118

Micro-Guard Cartridges
1250118***
De-Ashing Refill Cartridges,
30 x 4.6 mm, 21250119
Carbo-P Refill Cartridges,
30 x 4.6 mm, 21250128
Carbo-C Refill Cartridges,
30 x 4.6 mm, 21250129
Cation H Refill Cartridges,
30 x 4.6 mm, 21250506
Anion CO3 – Cartridges,
30 x 4.6 mm, 21250508
Cation Na+ Cartridges,
30 x 4.6 mm, 2

Sugars withorganic acids,
biofuels
Oligosaccharides,
thickening agents
Oligosaccharides

Price, U.S. $Applications

Ionic
Form

pH
Range

Price, U.S. $Aminex silver- and lead-formcolumns
Aminex HPX-87P column

H+ and
CO3–
Lead

6–8

340.005–9450.00Aminex calcium- andCalcium
potassium-form columns
Aminex hydrogen-form
Hydrogencolumns
Aminex silver- and lead-form CO3–
columns
Aminex HPX-87N column
Sodium5–9

310.00

1–3

320.00—510.00

5–9

365.00continues

138 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Prepacked Chromatography Columns

bio-rad.com/columns

Ordering Information
Catalog #Description

Contents

MW

1250585

Carbohydrate
Analysis Standard,
6 vials

Melezitose
MaltoseGlucoseMannoseFructose
Adonitol (ribitol)

504
360
180
180
180
152

Aminex HPX-87H column;
carbohydrate analysis kit

165.001250586

Organic Acid
Analysis Standard,
6 vials

Sodium oxalate
Sodium citrate
Sodium malate
Sodium succinate
Sodium formate
Sodium acetate

134
294
196
270
69
82

Aminex HPX-87H column;
organic acid analysis kit

165.00Catalog #Descriptionpl

For Use with

Price, U.S. $Standards

Price, U.S. $Cartridge Holders and Accessories
1250131
Standard Cartridge Holder, for one 30 x 4.6 mm cartridge
1250147
Cartridge Holder Seal Replacement Kit, for #1250131
1250148
Cartridge Holder Seals, for #1250131
1250139
De-Ashing Cartridge Holder, for #1250118, holds two 3.0 x 4.6 mm cartridges in series
7800008
Fittings, 10-32 PEEK, pkg of 2, black

695.00290.00135.00875.0020.00*			Supplied as 2 guard columns.
**		Includes 1 column and 2 Micro-Guard cartridges; requires standard cartridge holder, catalog #1250131.
*** Requires de-ashing cartridge holder, #1250139.

See Also

Econo-Pac Affinity and Desalting Columns
Econo-Pac prepacked columns for gravity-flow
chromatography allow fast and easy desalting as wellas simplified antibody purification.
Econo-Pac Protein A columns are well suited for bindingof lgGs, especially from mammalian species. The Econo-Pac
10DG media, with a molecular exclusion limit of 6,000, is
recommended specifically for desalting and buffer exchange.
The prepacked Econo-Pac columns include an upper frit,a snap-off end tip, graduated column markings, a 10 ml
bed, and 30 ml total column volume. To improve column
performance, use a flow adaptor, see below. For bottled
size exclusion resins, see pages 117–118; for bottled
affinity resins, see pages 106–110 and 111–116; andfor activated affinity resins, see pages 109 and 116.

Affi-Gel Protein A
MAPS II kit: page 112.

End cap

Affi-Gel Protein A
resin: page 112.
DEAE Affi-Gel Blue
resin: page 113.

20 ml ­reservoir
Upper porous­polymer bed
support

14 cm

10 ml bed ­volume
Lower porous
polymerbed support
Luer end fittingwith snap-off tip

Ordering Information
Catalog #Description

7322022732202073220107380019

Econo-Pac Protein A Columns, prefilled with Affi-Gel Protein A media, 5Econo-Pac Protein A Kit, 1 x 2 ml Affi-Gel Protein A column, 1 x 10 ml 10DG column, buffers
Econo-Pac 10DG Desalting Columns, pack of 30
Econo-Pac Flow Adaptor, 1.5 cm column ID

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $540.00330.00195.00140.00Chromatography: Laboratory and Process Separations | 139

Chromatography Columns
Empty Columns

bio-rad.com/spincolumns

Empty Columns
Bio-Rad’s empty columns accommodate a range of chromatography needs, including spin columns, low-pressurecolumns with optional flow adaptors and jackets for temperature control, and analytical columns for medium- or
high-pressure systems.

See Also
Nucleic acid sample
preparation: page 9.
Prepacked Bio-Spinand Micro Bio-Spincolumns: page 134
Empty EconoFit
columns: page 127.

Bio-Spin, Micro Bio-Spin, and Mini Bio-Spin Columns
Empty Bio-Spin and Micro Bio-Spin chromatography
columns are disposable polypropylene spin columns thatcan be packed with a variety of chromatographic media.
Bio-Spin columns hold up to 1.2 ml of any resin and fit
in standard swinging bucket centrifuges; Micro Bio-Spinand Mini Bio-Spin columns hold up to 0.8 and 1.2 ml ofresin respectively, and fit in standard microfuges. All threecolumns fit standard collection tubes, have snap-off tips
and polyethylene bed supports, and are autoclavable.
For More Information
Web: bio-rad.com/spincolumns
Download bulletin: 2289

End cap
Reservoir

5 cm

End cap

Reservoir

Reservoir

3.7 cm working bed height

3.3 cm

3 cm

1.2 ml bed volume

2.8 cm working bed height
2 cm working bed height

Porous 30 µm
polyethylene bed
support retains
fine particles

1.2 ml bed volume
0.8 ml bed volume
Reversible luer end
fitting with snap-off tip

Luer end fittingwith snap-off tip

Mini Bio-Spin Column

Micro Bio-Spin Column

Luer end fittingwith snap-off tip
Bio-Spin Column

Ordering Information
Catalog #Description

732600873260257326204
7326207
7311660

Bio-Spin Chromatography Columns, empty, 100
Bio-Spin Chromatography Columns, empty, 1,000
Micro Bio-Spin Chromatography Columns, empty, 100
Mini Bio-Spin Chromatography Columns, empty, 100
End Caps, for Micro Bio-Spin chromatography columns, 1,000

140 | Chromatography: Laboratory and Process Separations

Price, U.S. $170.001,540.00
145.00Inquire225.00In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Empty Columns

bio-rad.com/econopaccolumns

See Also

Econo-Pac Columns
Econo-Pac columns are 14 cm high, 1.5 x 12 cm polypropylene
columns that may be fitted with a flow adaptor (see below)
or used for gravity-flow chromatography. When used for open
column work, a special upper bed support prevents the bed
from running dry. Bed volumes from 1–20 ml are acceptable.
These columns can be autoclaved and will retain fine particles.
Columns can be easily stored in poly column racks.

Prepacked
Econo-Pac columns:
page 139.
End cap
10 ml ­reservoir

Graduated volume ­markings

14 cm

For More Information
Web: bio-rad.com/econopaccolumns
Download bulletin: 2289

11.5 cm working bed height

0–20 ml bed volume

Porous 30 µm polyethylene bed
support retains fine particles
Poly Column Rack

1.5 cm ID

Luer end fitting with snap-off tip

Ordering Information
Catalog #Description

73210107321011 cono-Pac Chromatography Columns, empty, includes upper bed supports, end caps, tip closures, 50
EEcono-Pac Chromatography Columns, empty, includes upper bed supports, end caps, tip closures, 500

Accessories
73800197317005
73281027318232
7311660

Econo-Pac Flow Adaptor, 1.5 cm column ID
Poly Column Rack, 20-place, with removable tube rack2-Way Stopcocks, female-to-male luer, 10
Female Luer Plugs, 25, polypropylene
End Caps, for Econo-Pac columns, 1,000

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $145.001,330.00
140.00450.0055.00
37.00
225.00Chromatography: Laboratory and Process Separations | 141

Chromatography Columns
Empty Columns

bio-rad.com/econocolumns

Poly-Prep Columns
Poly-Prep columns are 9 cm high, conical 0.8 x 4 cm
polypropylene columns that hold up to 2 ml ofchromatography resin and 10 ml of eluent or sample in
an integral reservoir. These columns are ideal for sample
preparation and small-scale chromatography applications,
including work with radioisotopes and other applications
that require disposable products. Poly-Prep columns may
be autoclaved and will retain fine particles.
For More Information
Web: bio-rad.com/polyprepcolumns
Download bulletin: 2289

End cap

10 ml ­reservoir

9 cm

3.9 cm working bed height
2 ml bed volume
Porous 30 µm polyethylene bed
support retains fine particles

Luer end fitting with snap-off tip

Ordering Information
Catalog #Description

7311550
7311553

Poly-Prep Chromatography Columns, empty, includes end caps and tip closures, 50
Poly-Prep Chromatography Columns, empty, includes end caps and tip closures, 1,000

Accessories
7311555
7317005
73281027318232
7311660

Poly-Prep Column Stack Cap, 50
Poly Column Rack, 20-place, with removable tube rack2-Way Stopcocks, female-to-male luer, 10
Female Luer Plugs, polypropylene, 25
End Caps, for disposable plastic columns, 1,000

142 | Chromatography: Laboratory and Process Separations

Price, U.S. $88.00
1,330.00
43.00
450.0055.00
37.00
225.00In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
bio-rad.com/econocolumns

Empty Columns

See Also

Glass Econo-Column Columns
Econo-Column chromatography columns are the standard
for high-quality, affordable low-pressure chromatography
columns. Columns ranging from 5 to 170 cm in lengthand 0.5–5.0 cm in diameter are available. A porous
polymer bed support at the bottom of the column retains
fine particles, and translucent polypropylene end fittings
allow viewing of the entire column bed. Econo-Column
chromatography columns can be autoclaved and aredesigned to operate at pressures <1 bar (14.7 psi).
Econo-Column chromatography columns accept EconoColumn funnels as well as Econo-Column flow adaptors.
Econo-Column chromatography columns are packaged inquantities of 1, 2, and 4, depending on column diameterand length. For convenience, all packaging sizes containone stopcock per column ordered.

Empty EconoFit
columns: page 127.
Translucent column ends
with male luer lock fittings to
simplify tubing connections

Column reservoir for
manual sample ­application

Accepts accessory
flow adaptors, funnels,
and reservoirs

Borosilicate glass ­barrel —clear, inert, durable

Bed support (28 µm)
retains fine ­particles
End caps for ­stopping
eluent flow or storing
packed ­column

For More Information
Web: bio-rad.com/econocolumns
Download bulletin: 2289

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 143

Chromatography Columns
Empty Columns

bio-rad.com/econocolumns

Ordering Information
Catalog #ID, cmLength, cm

Cross-Sectional
Area, cm2

Maximum
Volume, ml

Columns/Pkg

7370507
737051273705177370707
7374706
73707127374711
73707177370732
7370752
7374006
7371007
73740117371012737402173710227371032
7371052
7371091
73710937374150
7371507
7374151
73715127374156
73715177374152
73715227374153
7371532
7371552
7371576
7371591
73715937371598
7372507
7374251
7372512737425273725227374253
7372532
7372551

0.5
0.5
0.5
0.7
0.7
0.7
0.7
0.7
0.7
0.7
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5

5
10
15
5
5
10
10
15
30
50
5
5
10
10
20
20
30
50
100
120
5
5
10
10
15
15
20
20
30
30
50
75
100
120
170
5
10
10
20
20
30
30
50

0.20
0.20
0.20
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.79
0.79
0.79
0.79
0.79
0.79
0.79
0.79
0.79
0.79
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
1.77
4.91
4.91
4.91
4.91
4.91
4.91
4.91
4.91

1
2
3
2
2
4
4
6
12
20
4
4
8
8
16
16
24
40
79
103
9
9
18
18
27
27
35
35
53
53
89
124
177
230
301
25
49
49
98
98
147
147
246

2
2
2
2
4
2
4
2
2
2
4
2
4
2
4
2
2
2
2
2
4
2
4
2
4
2
4
2
4
2
2
2
2
2
2
2
4
2
4
2
4
2
2

Price, U.S. $
57.00
57.00
57.00
98.00
57.00
98.00
57.00
57.00
57.00
98.00
57.00
98.00
57.00
98.00
57.00
57.00
57.00
140.00175.00150.0082.00
150.0082.00
150.0082.00
150.0082.00
150.0082.00
94.00
125.00140.00175.00250.0082.00
150.0082.00
150.0082.00
170.0094.00
125.00continues

144 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Empty Columns

bio-rad.com/econocolumns

Ordering Information
Catalog #ID, cmLength, cm

Cross-Sectional
Area, cm2

Maximum
Volume, ml

Columns/Pkg

7372576
7372591
73725937375011737502173750317375051
7375071

2.5
2.5
2.5
5.0
5.0
5.0
5.0
5.0

75
100
120
10
20
30
50
70

4.91
4.91
4.91
19.63
19.63
19.63
19.63
19.63

344
491
589
196
393
589
982
1,374

2
2
2
1
1
1
1
1

Catalog #Price, U.S. $140.00195.00240.00140.00175.00210.00230.00325.00Description

Econo-Column Selection Packs
7376601
Econo-Column Selection Pack A, includes 7 columns, 1 each of 0.7 x 10, 20, and 30 cm; 		
1.5 x 30 and 50 cm; 2.5 x 20 and 50 cm
7376607
Econo-Column Selection Pack B, includes 6 columns, 1 each of 1.0 x 20, 30, and 50 cm; 		
1.5 x 20, 30, and 50 cm

275.00240.00For fittings, see low-pressure fittings on page 169.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 145

Chromatography Columns
Empty Columns

bio-rad.com/econocolumns

Econo-Column Flow Adaptors
Flow adaptors significantly improve column performance byeliminating head space above the gel bed and by protecting
the column bed from disruption during sample loading.
Flow adaptors improve resolution by delivering buffer andsample directly to the top of the column bed. Flow adaptors
are recommended for use with any low-pressure column
connected to pumps, the BioLogic LP system, or other lowpressure systems.

Econo-Column flow adaptors are available for 1.0, 1.5, 2.5,
and 5.0 cm ID Econo-Column chromatography columns(pages 143–145) and Econo-Pac columns (page 141).
Due to wear and tear of bed supports and O-rings,
maintenance kits are available for standard upkeep offlow adaptors.
3-position cam (1 = loose,
2 = sliding seal, 3 = fixed seal)

Delrin housing (snaps onto Econo-Column
columns or Econo‑Pac columns)

Reliable ethylene-propylene seal

Replaceable porous polymer bed support

For More Information
Web: bio-rad.com/econocolumnaccessories
Download bulletin: 2289

Replaceable flexible silicone tubing(0.88 mm ID) with replaceable female
luer lock fitting

Ordering Information
Catalog #Description

Flow Adaptors
738001473800157380016738001773800187380019

Flow Adaptor, 1.0 cm column ID, 1–7 cm functional length
Flow Adaptor, 1.0 cm column ID, 1–14 cm functional length
Flow Adaptor, 1.5 cm column ID, 1–14 cm functional length
Flow Adaptor, 2.5 cm column ID, 1–14 cm functional length
Flow Adaptor, 5.0 cm column ID, 1–14 cm functional length (does not include cam mechanism)
Econo-Pac Flow Adaptor, 1.5 cm column ID

140.00140.00140.00140.00655.00140.00Flow Adaptor Maintenance Kits
7380022
Flow Adaptor Maintenance Kit, for 5.0 cm flow adaptor, includes 2 bed supports and 2 O-rings
7380024
Flow Adaptor Maintenance Kit, for 1.0 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings
7380025
Flow Adaptor Maintenance Kit, for 1.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings
7380027
Flow Adaptor Maintenance Kit, for 2.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings

57.00
57.00
57.00
57.00

146 | Chromatography: Laboratory and Process Separations

Price, U.S. $In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Empty Columns

bio-rad.com/econocolumns

Econo-Column Funnel
The Econo-Column funnel, constructed of durable
polypropylene, is ideal for packing columns, loading diluted
samples, or delivering large volumes of buffer. It forms atight seal with Econo-Column chromatography columns
up to 2.5 cm ID (pages 143–145), Poly-Prep columns(page 142), and Econo-Pac columns (page 141).

Graduated volume
markings

250 mlreservoir

Cap with maleluer lock fitting

Ordering Information
Catalog #Description

7310003

Econo-Column Funnels, 250 ml, 5Price, U.S. $95.00

Glass Econo-Column Reservoirs
Reservoirs are available in 500 ml and 1 L capacities
and will fit 0.5, 0.7, 1.0, or 1.5 cm ID Econo-Column
chromatography columns. To make a constant-pressure
reservoir, close the reservoir top with a stopper that contains
a piece of glass tubing extending into the reservoir. The
removable upper cap has a male luer lock fitting.

Glass Econo-Column Reservoir

Ordering Information
Catalog #Description

73791127379113

Econo-Column Reservoir, 500 ml
Econo-Column Reservoir, 1 L

Price, U.S. $210.00215.00

Econo Alpha Empty Glass Chromatography Columns
Econo Alpha Columns are empty glass columns for low- to
medium-pressure chromatography. They are convenient,easy to pack with your resin of choice, and compatible
with common aqueous buffers used in protein purification.
The fully assembled columns are available in a range ofdimensions to fit your applications.
Features and Benefits
Pack your resin in a column size of your choice
■

■

■

■

Econo Alpha Empty Glass Chromatography Columns

Robust and easy-to-pack hardware
 ompatible with common aqueous buffers used inCprotein purification
A wide range of accessories available for order

Applications and Uses
Low- to medium-pressure chromatography■■■ ompatible with NGC Systems and otherC
chromatography systems
Columns come with preinstalled 10–32 and 1/4–28 fittings

For More Information
Web: bio-rad.com/econo-alphaDownload bulletin: 7317

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 147

Chromatography Columns
Empty Columns

See Also

Ordering Information

Bio-Scale CHT Type I
columns: page 136.

Catalog #Description

UNO columns:
page 136.

12009427
12009428
12009429
12009430
12009461
12009462
12009463
12009492
12009493
12009494
12009484
12009485
12009486
12009487
12009495
12009496
12009497
12009498
12009499
12009500
12009501
12009464
12009465
12009466
12009467
12009468
12009469
12009470
12009471
12009472
12009473
12009474
12009475
12009476
12009477
12009478
12009479
12009480
12009481
12009482
12009483
12009488
12009489
12009490
12009491

Econo Alpha Column, 15 x 500 mm
Econo Alpha Column, 25 x 500 mm
Econo Alpha Column, 10 x 100 mm
Econo Alpha Column, 15 x 100 mm
Econo Alpha Column, 25 x 100 mm
Econo Alpha Column, 10 x 500 mm
Econo Alpha Column, 6.6 x 50 mm
Econo Alpha Adjustable Plunger, 15 mm
Econo Alpha Adjustable Plunger, 10 mm
Econo Alpha Adjustable Plunger, 25 mm
Econo Alpha Packing Sleeve, 15 mm
Econo Alpha Packing Sleeve, 25 mm
Econo Alpha Packing Sleeve, 10 mm
Econo Alpha Packing Sleeve, 6.6 mm
Econo Alpha Column Extension, 15 x 500 mm
Econo Alpha Column Extension, 25 x 500 mm
Econo Alpha Column Extension, 10 x 100 mm
Econo Alpha Column Extension, 15 x 100 mm
Econo Alpha Column Extension, 25 x 100 mm
Econo Alpha Column Extension, 10 x 500 mm
Econo Alpha Column Extension, 6.6 x 50 mm
Econo Alpha Frit, 15 mm, 5 μm
Econo Alpha Frit, 15 mm, 10 μm
Econo Alpha Frit, 15 mm, 20 μm
Econo Alpha Frit, 15 mm, 30 μm
Econo Alpha Frit, 15 mm, 50 μm
Econo Alpha Frit, 25 mm, 5 μm
Econo Alpha Frit, 25 mm, 10 μm
Econo Alpha Frit, 25 mm, 20 μm
Econo Alpha Frit, 25 mm, 30 μm
Econo Alpha Frit, 25 mm, 50 μm
Econo Alpha Frit, 10 mm, 5 μm
Econo Alpha Frit, 10 mm, 10 μm
Econo Alpha Frit, 10 mm, 20 μm
Econo Alpha Frit, 10 mm, 30 μm
Econo Alpha Frit, 10 mm, 50 μm
Econo Alpha Frit, 6.6 mm, 5 μm
Econo Alpha Frit, 6.6 mm, 10 μm
Econo Alpha Frit, 6.6 mm, 20 μm
Econo Alpha Frit, 6.6 mm, 30 μm
Econo Alpha Frit, 6.6 mm, 50 μm
Econo Alpha O-Rings, 15 mm
Econo Alpha O-Rings, 25 mm
Econo Alpha O-Rings, 10 mm
Econo Alpha O-Rings, 6.6 mm

NGC systems:
page 150.
ENrich columns:
page 125.

bio-rad.com/econocolumns

148 | Chromatography: Laboratory and Process Separations

Price, U.S. $210.00215.00In the U.S., call toll free at 1-800-424-6723

Chromatography Columns
Empty Columns

bio-rad.com/econocolumns

Bio-Scale MT High-Resolution Columns
Bio-Scale MT empty columns can be packed with theresin of your choice. These columns provide extremely
high resolution in most chromatography applications.
Bio-Scale MT columns allow precise sample applicationand provide the low dead volume required for highresolution separations. The four column sizes (2, 5,
10, and 20 ml) allow easy scale-up of separation andpurification protocols. The optimized design allows easy
packing, bed height adjustment, sample application,and equilibration. Bio-Scale MT columns are convenient
for use with BioLogic systems or any medium- or highpressure chromatography system.
For More Information
Web: bio-rad.com/MTcolumns
Download bulletin: 1970

Adjustable bed support eliminates column voids
Easy assembly for packing and cleaning

Biocompatible­materials for­preservation of­sample biologicalactivity

Optimized design of sample applicator for
high-resolution chromatography
Fingertight assembly
eliminates the needfor tools

Transparent
glass tube allows
easy bed inspection

Ordering Information
Catalog #Description

Bio-Scale Columns*
7510081
Bio-Scale MT2 Column, 7 x 52 mm
7510083
Bio-Scale MT5 Column, 10 x 64 mm
7510085
Bio-Scale MT10 Column, 12 x 88 mm
7510087
Bio-Scale MT20 Column, 15 x 113 mm
Catalog #Column Volume, ml Pressure Limit, psi
1.9–2.3
4.6–5.7
9.5–11.3
19.4–21.9

1,000
750
600
500

Description

Price, U.S. $685.00715.00740.00840.00Price, U.S. $Cartridge Holders and Accessories
7510091
Bio-Scale 2 Replacement Parts Kit, includes 5 frits, 5 distribution screens, 2 O-rings, 1 frit remover
7510093
Bio-Scale 5 Replacement Parts Kit
7510095
Bio-Scale 10 Replacement Parts Kit
7510097
Bio-Scale 20 Replacement Parts Kit
7500554
1/16" OD (1.6 mm) Post-Pump Fittings, includes Delrin nut, ferrules, lock ring, 10 sets
7500567
UNO M6 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an FPLC system
7500568
UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use with
BioLogic DuoFlow and NGC systems

125.00125.00125.00125.00185.0036.00
47.00* Each Bio-Scale MT column comes with a Bio-Scale replacement parts kit.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 149

Medium-Pressure Chromatography Systems
bio-rad.com/NGCsystems

Medium-Pressure Chromatography Systems
See Also
Multi-D
chromatography
applications:page 152.

NGC Medium-Pressure Chromatography Systems
The NGC family of medium-pressure preparative chromatography systems offers a single laboratory chromatography
solution that aligns and scales to fit your purification, automation, and throughput requirements. NGC laboratory scalechromatography systems provide you with a fully customizable platform that can be designed to your current purificationneeds and is upgradable to meet future throughput and automation requirements.
For More Information
Web: bio-rad.com/NGCsystems
Please contact your local Sales Representative for more details.
Learn More about the Technology
Web: bio-rad.com/tech/chrom

NGC Scout PlusNGC Discover

NGC Discover Pro

NGC Fraction Collector

NGC chromatography system platforms — customizable, and upgradable systems that adapt and expand to your throughput
and application needs. All NGC systems are compatible with the NGC fraction collector and BioFrac fraction collector.

NGC Fraction Collector(see page 162)

150 | Chromatography: Laboratory and Process Separations

BioFrac Fraction Collector(see page 164)

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
bio-rad.com/NGCsystems

ll

ll
ll

NGC Discover 100 Pro
7880012NGC Discover 10 Pro
78800117884018ll

NGC Discover 100
7880010NGC F100 Pump Module

NGC Discover 1078800097884003ll

NGC Scout 100 Plus
7880008

NGC F10 Pump Module

NGC Scout 10 Plus
7880007

Product Description

7884002

NGC Quest 100 Plus
7880004

Catalog #NGC Quest 10 Plus
7880003

NGC Chromatography Systems Selection Guide

ll
ll

ll

NGC Mixer Module

l

l

l

l

l

l

l

l

7884007

NGC Sample Inject Valve Module

l

l

l

l

l

l

l

l

12010343

NGC Multi-Wavelength Detector II Module,
includes conductivity monitor

l

l

l

l

l

l

l

l

7884010

NGC Buffer Blending Valve Module

l

l

l

l

l

l

l

l

7884011

NGC pH Valve Module, includes pH probe

l

l

l

l

l

l

l

l

7884004

NGC Sample Pump Module, integrated

l

l

l

l

l

l

l

l

7884006

NGC Inlet Valve Module

l

l

l

l

ll

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lll

lll

7884012NGC Column Switching Valve Module, 10 mll

7884026

NGC Column Switching Valve Module, 100 ml

7884013

NGC Outlet Valve Module

l

l

l

l

l

l

l

l

12009390

ChromLab Software

l

l

l

l

l

l

l

l

l

l
l

l
l

l

l

l  Standard l  Optional
More than one dot indicates that a system comes with more than one module.
Note: All NGC Systems include a touch screen and NGC Fittings Kit (#7884017) and are compatible with the NGC Fraction Collector and BioFrac Fraction Collector.

Features and Benefits
Flexible, customizable chromatography systems to suit
both your application and research needs.
■

■

■

■

■

■

■

■

 djustable and scalable platform adapts to individualA
application, workflow, and throughput requirements
 hromLab software provides powerful, graphicalC
instrument control, streamlined method development,
and intuitive data analysis
 raphical fluidics scheme selector matches the flowGpath to applications-based system setup
 oint-to-Plumb feature lighting provides step-by-stepP
LED guided setup for easy system plumbing
 eal-time active flow path display for clear visualizationRof buffer, sample, and valve position and easy
identification of system status
 eal-time status displays offer immediate system
Rdiagnostics
 re-plumbed systems with QC-validated performanceP
support more reproducible results and sharper peaks(included with all preconfigured NGC Quest, NGC Scout,and NGC Discover systems)

■

■

■

 pen platform is compatible with all medium-pressureOchromatography columns and resins
 ompatibility of NGC and BioFrac fraction collectors
C
allows for collection from analytical to preparative
scale purifications
 ompact footprint fits on lab bench, in deli-fridge,
Cor in coldroom

For more information about these features and benefits,
please contact your local sales representative. Take a tourof the system at bio-rad.com/NGCsystems.
Applications and Uses of Medium-Pressure
Chromatography Systems
Preparative to analytical scale isolation, purification,
and analysis of multiple types of molecules, including:■Recombinant protein purification and refolding■

Monoclonal antibody purification■

Virus removal preparations■

Analysis of plasma proteins for disease diagnosis■

Nucleic acid purifications

 ier-Rotate system design enables optimal placement ofTvalves and detectors to minimize hold-up volume

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 151

Medium-Pressure Chromatography Systems
bio-rad.com/NGCsystems

NGC Systems
Preconfigured NGC systems are designed with increasing automation and throughput in mind. All NGC systems include
either a 10 or a 100 ml/min automated dual gradient pump, a mixer, a sample injection valve, and a detector with aconductivity monitor. Each preconfigured system can be further customized with additional valves to meet your specificpurification needs.
NGC Quest Plus
NGC Quest Plus systems are designed for the all-purpose
purification of proteins with automated pumps thatprovide accurate gradients for high-resolution separations.
These systems support automated sample injection using
fixed or dynamic sample loops.
NGC Quest 10 Plus and Quest 100 Plus systems feature:■ ulti-wavelength (4) detector for simultaneous detection
M(190 to 800 nm) of proteins, peptides, nucleic acids,
chromophores, and other biomolecular complexes withhigh-sensitivity conductivity measurements for accurate
salt gradient formation

NGC Scout Plus
NGC Scout Plus systems are designed for method
optimization with automated pumps and valves thatprovide accurate gradients and pH for high-resolution
separations suitable for any application.

NGC Discover and Discover Pro
Both the NGC Discover and NGC Discover Pro
chromatography systems are designed for higher
throughput applications with rapid, robust automation
for those who need to do method development.
NGC Discover 10 and 100 systems include the capabilitiesof the NGC Scout 10 and 100 Plus instruments but also
comprise:■■■

NGC Scout 10 Plus and Scout 100 Plus systems include:
■

■

■

■

All features of NGC Quest Plus systems
 uffer blending to automate buffer titrations for rapidB
scouting of pH parameters ompatibility for gradient separations at different pHCvalues for rapid method development
Multi-wavelength (4) UV/Vis and conductivity detector

152 | Chromatography: Laboratory and Process Separations■

 100 ml/min integrated sample pump — supports
A
contamination-free automated large-volume
sample application
Inlet valves — enable automated switching between
buffers and samples, accelerating method development.
NGC supports up to 2 inlet valves for buffers and2 inlet valves for samples
 column switching valve — facilitates automatedA
column/resin scouting up to 5 columns without theneed to replumb. NGC supports up to 3 column
switching valves
 sample outlet valve, a standard feature with theA
NGC Discover Pro systems — allows collection of up
to 12 large-volume fractions and enables automatedMulti-D chromatography applications. NGC supports
up to 2 outlet valves

Learn More about Multi-D Chromatography
Web: bio-rad.com/Multi-D

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

Video

Ordering Information
Catalog #Description

Price, U.S. $NGC Medium-Pressure Chromatography Systems*
7880003
NGC Quest 10 Plus Chromatography System, includes automated 10 ml/min pumps, multi-wavelength(UV/Vis) and conductivity detection, accurate gradients, and sample injection
NGC Quest 100 Plus Chromatography System, includes automated 100 ml/min pumps, multi-wavelength
7880004(UV/Vis) and conductivity detection, accurate gradients, and sample injection
7880007
NGC Scout 10 Plus Chromatography System, includes NGC Quest 10 Plus capability, automated 10 ml/min
pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880008
NGC Scout 100 Chromatography System, includes NGC Quest 100 capability, automated 100 ml/min
pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880009
NGC Discover 10 Chromatography System, includes NGC Scout 10 Plus capability, automated 10 ml/min
pumps, sample pump for large sample injection, column switching valve (up to 5 columns), and buffer inlet valve
NGC Discover 100 Chromatography System, includes NGC Scout 100 Plus capability, automated 100 ml/min
7880010pumps, sample pump for large sample injection, column switching valve (up to 5 columns), and buffer inlet valve
7880011
NGC Discover Pro 10 Chromatography System, includes NGC Scout 10 Plus capability, automated 10 ml/min
pumps, sample pump for large sample injection, sample inlet valve, column switching valve (up to 5 columns),
2 buffer inlet valves, and outlet valve
7880012NGC Discover Pro 100 Chromatography System, includes NGC Scout 100 Plus capability, automated
100 ml/min pumps, sample pump for large sample injection, sample inlet valve, column switching valve(up to 5 columns), 2 buffer inlet valves, and outlet valve

Product
Demonstration

InquireInquireInquirebio-rad.com/
NGCMulti-D

InquireInquireInquireInquireInquire* All NGC systems include ChromLab software.

NGC Valve Overview
The NGC family of medium-pressure chromatography systems offers flexible system configurations that enable upgrades
and reconfiguration of the valves to fit multi-user needs, applications, and laboratory space requirements. Systems can beeasily reconfigured and seamlessly upgraded with increased functionality such as higher flow rates (10 ml/min or 100 ml/min),sophisticated detection capabilities, pH monitoring, automated buffer selection, multicolumn connection, and buffer blending.
NGC Buffer Blending ValveThe NGC buffer blending valve is used for automatic
online buffer preparation and generation of pH gradientsfor quick pH scouting. When used in conjunction withthe dual gradient pump system, the buffer blending
valve enables automated elution gradients and highly
accurate buffer blending for rapid scouting and method
development. The valve can also double the flow rate of asalt gradient on an F10 pump and F100 pump.
NGC Inlet ValveThe NGC inlet valve allows for automatic bufferand sample selection during method running. It speeds
up method development by allowing for automatic selectionof up to seven buffers and a cleaning solution per valve.
It also works as a mini autosampler for injecting multiple
large-volume samples via the sample pump.

In the U.S., call toll free at 1-800-424-6723

NGC Buffer Blending ValveNGC Inlet Valve

Chromatography: Laboratory and Process Separations | 153

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

NGC F10 and F100 System Pump
The F10 and F100 pumps produce buffer gradientsolutions. Flow rates on all pumps can be automaticallycontrolled to avoid overpressure. NGC instruments canhave up to three high-precision pumps: two system
gradient pumps (pump A and pumb B) and one
sample pump.
NGC Sample Pump 100
The NGC sample pump 100 enables both the injectionof large sample volumes and accurate and consistentmultiple loadings from a single sample stock. This
dedicated pump eliminates the risk of contaminating
system pumps, allowing for automated loading of large
sample volumes directly to the column. The sample pumpalso includes an integrated pressure sensor that protects
the column and resin from overpressure.
NGC Sample Inject ValveThe NGC sample inject valve enables the system to loada specific predetermined volume of sample onto a column
with a maximum operating pressure of 3,650 psi. It isalso compatible with capillary loops and sample pumps,
eliminating the need to replumb.

NGC Sample Pump

Specifications for System and Sample Pumps
F10 Pump
Flow rates	0.001–10 ml/min (normal range);
0.002–20 ml/min (column packing flow)
Max pressure limit

3,650 psi (252 bar, 25.2 MPa)

F100 Pump
Flow rate setting	0.01–100 ml/min (normal range);
0.02–200 ml/min (column packing flow)
Max pressure limit

1,450 psi (100 bar, 10 MPa)

Sample Pump 100
Flow rate	Up to 100 ml/min

NGC Multi-Wavelength Detector II
The NGC UV/Vis detector is combined with an integrated
conductivity monitor to measure buffer conductivity orsalt gradients.

Max pressure limit

1,450 psi (100 bar, 10 MPa)

The multi-wavelength UV/Vis detector adds flexibility to
the chromatography system, enabling the simultaneous
monitoring of four wavelengths (UV and Vis) for greater
sensitivity and detection of proteins, peptides, nucleic
acids, and chromophores such as hemoglobin.
NGC Outlet Valve
Use the NGC outlet valve either as a stand-alone unit
or in conjunction with the BioFrac and NGC FC
fraction collectors.
As a single, stand-alone unit, the outlet valve is capableof collecting up to 12 large-volume fractions such as
flowthrough or waste. Two outlet valves may be daisychained to collect up to 23 large-volume fractions,
including waste.
When a BioFrac or NGC FC fraction collector isconnected, the collection sequence can be programmed
to collect smaller-volume fractions in the fraction collector
and larger-volume fractions via the outlet valves, providing
full flexibility.
The outlet valve also enables automated tandem/2-D andMulti-D chromatography applications by directing peaksof interest onto the next column.

NGC Multi-Wavelength Detector II

Specifications for NGC Multi-Wavelength Detector II
UV Monitor
Multi-wavelength (up to 4) range

Conductivity Monitor
Conductivity range	0.01–999.99 mS/cm

Features and benefits of the NGC outlet valve include:■

Collect up to 12 large-volume fractions with a single valve■

Collect up to 23 large-volume fractions with two valves■■

154 | Chromatography: Laboratory and Process Separations

190–800 nm

 ollect variable fraction sizes with the BioFrac and NGC
C
FC fraction collector
Automate multistep purifications

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

NGC pH ValveThe NGC pH valve module includes a pH electrodefor accurate pH monitoring during the run (1–14).
The valve is capable of directing flow to the pH electrodeor bypassing the probe automatically. The valve includes
an integrated calibration port, which can be used tocalibrate the probe without disconnecting the probefrom the system.

NGC pH Valve

For sensitive applications, the software is capable ofcalculating and displaying temperature-compensated pH
values for accuracy and ease of use.
NGC Mixer
The NGC mixer homogenizes buffers from two system
pumps (Pumps A and B). In addition to the mixer motor
assembly, the mixer includes an integrated systempressure sensor. It can accommodate both F10 mixers(263 µl and 750 µl) and F100 mixers (2, 5, and 12 ml).
The mixer volume can be adjusted, depending on flow
rate, to achieve optimal buffer homogenization by inserting
the appropriate size barrels.
NGC Air Sensor
The NGC air sensor enables detection of the end of bufferand sample, thereby protecting against air entering thesystem and damaging pumps or columns. The module
supports up to four air sensors (large- and small-bore).
Each NGC air sensor extension module can be used inconjunction with the air sensor module to enable four
additional air sensors (totaling up to eight).
NGC Column Switching ValveThe NGC column switching valve allows connection of up
to five columns, enabling quick and easy column scouting
without replumbing. With an internal bypass mode thisvalve lets buffers bypass the connected columns when
priming or cleaning the system. The valve can also
reverse the flow, which is ideal for column cleaning andapplications that require narrow band elution.
The column switching valve has integrated pressure
sensors that measure pre- and delta-column pressures.
This protects the column and resin from overpressure by
triggering the pumps to slow down or stop. The column
switching valve can also be used to connect sample loops
in multistep purifications.
Signal Import Module
Adding external devices to a purification workflow provides
the flexibility to increase automation and the amount of
information collected. The NGC signal import module (SIM)enables analog to digital signal conversion and connection
to third-party devices such as external detectors or
autosamplers.

NGC Air Sensor Module

NGC High-Flow
Tubing Kit

NGC Accessories
NGC Fraction Collector
The NGC fraction collector’s flexibility and versatility improves
high-throughput purification workflows. It can collect into
multiple vessel types within a method and has increased
capacity in comparison to the BioFrac fraction collector.
The NGC chromatography system paired with the NGC
fraction collector provides collection options for analytical topreparative chromatography applications and is well suited
to meet the purification needs of industry laboratories. Pleaserefer to page 162 for detailed product information.
BioFrac Fraction Collector
The BioFrac fraction collector is compatible with all
NGC chromatography systems. It is ideal for analyticalto preparative scale chromatography applications.
Please refer to page 164 for detailed product information.
NGC High-Flow Tubing Kit
Tubing kit for operating NGC 100 chromatography systems
at high flow rates. Recommended for operation of NGC 100
chromatography systems at flow rates greater than 40 ml/min.
■■■

In the U.S., call toll free at 1-800-424-6723

 nables the operation of NGC 100 chromatographyEsystems at flow rates greater than 40 ml/min
 ubing, in combination with a NGC buffer blending valve,Twill allow operation of NGC 100 chromatography systems
at flow rates up to 200 ml/min he kit contains 3/16” OD PFA tubing, connection adaptors,Tand bottle caps for buffer inlet lines and waste line

Chromatography: Laboratory and Process Separations | 155

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

Ordering Information
Catalog #Description

Price, U.S. $NGC Medium-Pressure Chromatography System Pumps, Valves, and Accessories
System Pumps
7884002
NGC F10 Pump, includes 10 ml/min system pump kit for creating buffer gradients; can be used inconjunction with buffer blending valve to generate flow rates up to 20 ml/min; kit includes necessary
tubing and fittings
7884003
NGC F100 Pump, includes 100 ml/min system pump kit for creating buffer gradients; can be used inconjunction with buffer blending valve to generate flow rates up to 200 ml/min; kit includes necessary
tubing and fittings
Sample Pump
7884004
Detectors
12010343
12012532
12012531
12012533
788502478850237885022N GC Sample Pump 100, includes 100 ml/min sample pump kit for automated large-volume sample application via
the sample inject valve; kit includes necessary tubing and fittings
NGC Multi-Wavelength Detector II, for simultaneous wavelength (4) monitoring of elution fractions
190–800 nm and conductivity; UV/Vis, conductivity detector kit includes necessary tubing and fittings
UV/Vis and Conductivity Flow Cell, 5 mm, for the multi-wavelength detector II. Standard flow cell for all
NGC systems
UV/Vis and Conductivity Flow Cell, 10 mm, for the multi-wavelength detector II. Analytical flow cell forincreased sensitivity
UV/Vis and Conductivity Flow Cell, 2 mm, for the multi-wavelength detector II. Preparative flow cell for
decreased sensitivity
NGC UV Flow Cell, 5 mm, UV flow cell, standard with all systems. Fits the discontinued singlewavelength UV and discontinued multi-wavelength UV/Vis detectors in both F10 and F100 systems
NGC UV Flow Cell, 10 mm, analytical UV flow cell. Fits the discontinued single-wavelength UV anddiscontinued multi-wavelength UV/Vis detectors in F10 systems. Ideal for use at lower flow rates
NGC UV Flow Cell, 2 mm, preparative UV flow cell. Fits the discontinued single-wavelength UV anddiscontinued multi-wavelength UV/Vis detectors in F100 systems. Ideal for use at high flow rates

5,450.00

7,500.00

5,950.00

580.00580.001,450.00

Detector Lamp Replacements
7885000
NGC LED Lamp Replacement, includes 255/280 nm LED lamp replacement for single-wavelength detectors
7885001
NGC Deuterium Lamp Replacement, includes deuterium lamp replacement (UV) for multi-wavelength detectors
7885002
NGC Tungsten Lamp Replacement, includes tungsten lamp replacement (Vis) for multi-wavelength detectors

1,260.00
2,075.00
630.00

pH Valve
78840117885026

NGC pH Valve, for accurate inline pH measurement; includes pH probe, tubing, and fittings
 GC pH Probe (Ag/AgCl), for use with the pH valve for accurate pH monitoring (pH 0–14) during purifications
N

4,100.00
500.00NGC Mixer, for use with all NGC systems; additional mixer base or barrels (ordered separately) can be extended with
2, 5, and 12 ml barrels for efficient gradient mixing at higher flow rates
NGC F100 Mixer, 750 µl base and top assembly, included with all 100 ml/min NGC systems
NGC F10 Mixer, 263 µl base and top assembly, included with all 10 ml/min NGC systems
NGC F10 Mixer Barrel Kit, 750 µl extension barrel for F10 263 µl mixer (part of Scout 10 and Discover 10 series)
NGC F10 Mixer Barrel Kit, 2 ml extension barrel for F10 263 µl mixer (optional)
NGC F100 Mixer Barrel Kit, 2 ml extension barrel for F100 750 µl mixer body (part of Scout 100 andDiscover 100 series)
NGC F100 Mixer Barrel Kit, 5 ml extension barrel for F100 750 µl mixer (optional)
NGC F100 Mixer Barrel Kit, 12 ml extension barrel for F100 750 µl mixer (optional)

2,100.00

 GC Inlet Valve, for automated switching between multiple buffers and samples during methods, kit includes theNnecessary tubing and fittings
NGC Sample Inject Valve, for manual sample application of small-volume samples via sample loops orlarge-volume samples using a sample pump; kit includes necessary tubing, fittings, and sample injection port;
valve can also be used with DynaLoops and sample pumps
NGC Buffer Blending Valve, for online buffer preparation and generating pH gradients for quick pH scouting; kit
includes the necessary tubing and fittings; can also double system pump flow rate

3,200.00

Mixer
78840187884019788402078840217884022788402878840237884024
Valves7884006
7884007

7884010625.00210.0099.00
99.00
250.00250.00450.002,600.00

5,500.00
continues

156 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

Ordering Information
Catalog #Description

Price, U.S. $Valves (continued)
7884012
NGC Column Switching Valve, 10 ml, for use with F10 systems and multiple columns for quick column scouting
and reverse flow, holds 5 columns; kit includes the necessary tubing and fittings to accommodate the most common
column types
7884013
NGC Outlet Valve, for collection of up to 12 large-volume fractions, including necessary tubing and fittings

3,000.00

7884026

5,500.00

 GC Column Switching Valve, 100 ml, for use with F100 systems and multiple columns for quick column scoutingN
and reverse flow, holds 5 columns; kit includes the necessary tubing and fittings to accommodate the most common
column types

Maintenance Kits
7885003
Pump Maintenance Kit, 10 ml, for regular maintenance of NGC F10 pumps, includes seals and check valves
7885004
Pump Maintenance Kit, 100 ml, for regular maintenance of NGC F100 pumps, includes seals and check valvesPump Maintenance Kit, 100 ml, for regular maintenance of NGC sample pump, includes seals and7885005
check valves
Air Sensor
7885017788501878850207885021

NGC Air Sensor Module, for detection of end of buffer and sample, protects against air entering pumps andcolumns, supports up to 4 air sensors (large- and small-bore); kit includes 2 large-bore air sensors
NGC Air Sensor Extension Module, connects to the base air sensor module to support 4 additional air sensors(optional, does not include any air sensors)
NGC Air Sensor, small, includes air sensor; enables exclusion of air from system and columns.
Detects air in small-diameter PEEK tubing
NGC Air Sensor, large, includes air sensor; enables exclusion of air from system and columns.
Detects air in large-diameter PTFE tubing

Fraction Collectors
NGC Fraction Collector, 100/240 V, includes two 13 mm racks, tubing kit, external power supply,
17002070
and USB cableBioFrac Fraction Collector, 100/240 V, fraction collector compatible with all NGC systems, includes
7410002
power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac diverter valve, fittings kit
NGC Communication Adaptor, enables communication between Bio-Rad devices such as the BioFrac
7884025
fraction collector and the NGC system included with BioFracNGC Signal Import Module, enables analog to digital signal conversion and connection to devices
7884016
such as third-party autosamplers and external detectors, includes cable for connecting to NGC system(#7885013) and external detectors
NGC Accessories
7885038
NGC Column Holder, magnetic column holder with one column clamp for use with NGC systems, pkg of 1
7885057
NGC Dual Column Holder, magnetic column holder dual column clamps with NGC systems, pkg of 1
7885039
NGC Cartridge Holder, universal cartridge holder, holds 1–10 ml cartridges
7885041
NGC Sample/Wash Tube Holder, holds two 50 ml Falcon tubes, for use with NGC systems (tubes not included)7885042
NGC Tubing Retainers (small), holds small PEEK tubing in an organized manner, pkg of 3 magnetic retainers
7885035
NGC Tubing Retainers (large), holds larger PTFE tubing in an organized manner, pkg of 3 magnetic retainers
7500703
NGC Inline Filter Kit, includes 1 inline filter and 2 replacement frits
7500704
Replacement Frits, pkg of 5, for inline filter kit
7884017
NGC Fittings Kit, pkg of 1, multiple fittings
750023040 psi Backpressure Regulator
7500566
Bottle Cap Kit, includes 2 bottle caps, 2 plugs, for use with 1/8" OD tubing on NGC systems
7885059
NGC Check Valves, pkg of 2
NGC Tubing
7500603
7500602
7600604
7600605
7885171

5,500.00

950.001,070.00
3,375.00

3,200.00
820.001,600.00
1,600.00

5,415.00
5,415.00
395.001,500.00

150.0068.00
68.00

160.00160.00950.00PTFE FEP Tubing, 1/8" (0.125", 3.2 mm) OD x 0.062" (1.6 mm) ID, 15' (4.6 m), for pre-pump buffer inlet lines to thepump heads
Tefzel Tubing, 1/16" (0.062", 1.6 mm) OD x 0.020" (0.5 mm) ID, 30' (9.1 m), for waste lines
PEEK Tubing, orange, 1/16" OD x 0.020" ID x 30', rated to 5,000 psi
PEEK Tubing, green, 1/16" OD x 0.030" ID x 30', rated to 3,000 psi
NGC High-Flow Tubing Kit, 3/16" OD PFA tubing with 5/16-24 fittings, 1/4-28 x 5/16-24 PEEK adaptors, 1/8" OD
PFA tubing with 1/4-28 fittings, bottle caps for 3/16" OD PFA tubing, 1/16" OD green PEEK fraction collector tubing
with 1/4-28 fittings
continues

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 157

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

Ordering Information
Catalog #Description

Price, U.S. $Single-Injection Sample Loops, Kits, and Accessories
7500471
Sample Injection Port, for use with NGC sample injection valve
1250224
Injection Needle, 22 gauge, blunt
12004633
Luer Injection Port, 5 pack of female luer to male 1/4-28 fittings
7500490
Small-Volume Sample Loop Kit, includes 100, 250, and 500 µl PEEK loops
7500491
Large-Volume Sample Loop Kit, includes 1, 2, and 5 ml PEEK loops
7500482
25 µl Tefzel Sample Loop
7500483
50 µl Tefzel Sample Loop
7500492
100 µl PEEK Sample Loop
7500493
250 µl PEEK Sample Loop
7500494
500 µl PEEK Sample Loop
7500495
1 ml PEEK Sample Loop
7500496
2 ml PEEK Sample Loop
7500497
5 ml PEEK Sample Loop

34.00
69.00
225.00300.00
59.00
65.00
74.00
74.00
74.00
74.00
99.00
135.00Additional Options
7884000

NGC Expansion Bay, tier 3, expands base system to 3-tier system for use with additional modules

2,500.00

7884001

NGC Expansion Bay, tier 4, expands system to 4-tier system for use with additional modules

2,500.00

7885014

NGC Buffer Tray, holds up to 8 buffer bottles. Includes drain port to prevent flooding in the event of a leak

7885016

NGC Drip Tray, for use with NGC systems

160.007885040

 GC Touch Screen Stand, used to mount touch screen outside of cold box or coldroom while tetheredNto the system, enables convenient system operation from the outside
Touch Screen Bracket, for use with standard third-party monitor stands, used to mount touch screen
outside the cold box or coldroom while tethered to the system, includes cableComputer, for use with NGC systems, includes Intel Core i7 3.4 GHz (quad core), 4 GB RAM,1 x 250 GB hard drive, CD/DVD drive, Microsoft Windows 7 Professional 64-bit edition
Monitor, for use with NGC system computer

555.007885060
788401578840277880020IQOQ

7880030IQOQ

 GC IQ/OQ, NGC Quest/Quest Plus IQ/OQ (with BioFrac or NGC fraction collector and air sensor),
N
Installation Qualification and Operational Qualification (IQ/OQ) performed by field service engineers on location
at the customer’s site, duration: 1 day
NGC Scout/Discover/Discover Pro IQ/OQ (with BioFrac or NGC fraction collector and air sensor),
Installation Qualification and Operational Qualification (IQ/OQ) performed by field service engineers on location
at the customer’s site, duration: 2–3 days

158 | Chromatography: Laboratory and Process Separations

160.00265.001,950.00
375.006,999.00

InquireIn the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

NGC ChromLab Software
ChromLab chromatography software is the integrated method development, data acquisition, and analysis software
package for all NGC chromatography systems. It controls all functions for lab-scale protein purification including
instrument control, method development, real-time monitoring, chromatogram comparison, and peak analysis.
This interface is also fully configured with virtual network computing (VNC), allowing you to control and monitor thesystem remotely from nearly any smartphone, tablet, or laptop.
ChromLab software is available in standard format or as a security edition, which provides the necessary features to permit
users to operate in compliance with Title 21 of the U.S. Code of Federal Regulations Part 11 (U.S. FDA 21 CFR Part 11)
within a closed system. It is compatible with Windows 7–10 64 bit.

ChromLab Software
ChromLab software is available in standard format.
Upgrades, which are free to existing users, containenhancements to existing ChromLab software features
and a linear flow rate calculator.

Fluidics Scheme

Graphical Instrument Setup and Control
ChromLab software instrument controls are designed
around a novel fluidics scheme interface that can becustomized to exact hardware configurations. ChromLab
software enables easy setup with its novel Point-to-Plumbfeature. During manual data acquisition, each componentof the fluidics scheme can be controlled to reach optimized
conditions. In both manual and automated method-basedruns, the software highlights the real-time fluidics path andmodule status to ensure accuracy.
The instrument control interface is touch-screen optimized,
allowing flexibility without the need for a computer adjacentto the system, thus minimizing the total footprint.
Streamlined Method Development
ChromLab software includes templates for common
chromatographic techniques and a column database for
maximum versatility. Select a column from the column
database. The software automatically adjusts optimal flowrate and pressure parameters for the selected column.

Click on each step in flow path for guided Point-to-Plumb LED system
plumbing. Instrument LEDs blink at the next plumbing location.

Methods are created by drag-and-drop functional
phases. Grouping parameters into intuitive phases puts
the focus on separation workflow instead of hardware
controls. The interactive gradient graph enables
visualization of the protocol and quick click-and-dragexperimental adjustments.

Manual Instrument Control
Active flow path(green) clearlyhighlighted
Real-time status
display of flow
path devices

Complete system status and control is available via the graphical fluidics scheme.

In the U.S., call toll free at 1-800-424-6723

Graphical manualcontrol panels
allow complete,accessible manualcontrol of thesystemChromatography: Laboratory and Process Separations | 159

Medium-Pressure Chromatography Systems
bio-rad.com/NGCaccessories

Automated Multi-D Purification Techniques
The software contains tandem and 2-D purification
templates that reflect common automated multistep
purification scenarios. These templates may be edited
to fit your research and reduce hands-on time.

Extensive column library
selection and intuitive interface
for custom self-packed columns

Features and Benefits of ChromLab Software
Touch screen allows the same level of flexibility without
the need for a computer adjacent to the system,
minimizing the total footprint for an operational system■■ ustomizable chromatogram layouts with a variety ofCviewing and data analysis options– Enhances multi-user control with user-specificpreferences for personalized analysis– Provides ultimate system control with the ability to editon the fly and save as a new method during current run

■

■

■

■

 apid creation of trace comparisons for easy analysis
R
across multiple purification runs
 ingle-click peak detection and integration forS
quicker results
 dvanced integration parameters to enable manualA
integration functions and peak addition or removal forfine-tuning your dataIntuitive, grouped data tabular layouts that organize data
for easy navigation

Applications and Uses of ChromLab Software
All functions of the NGC medium-pressure
chromatography system are controlled for lab-scaleprotein purification■■■

Method development to identify best methods eal-time monitoring of proteins, peptides, nucleic acids,Rand chromophores
Phase-based
method editing– Provides collection flexibility with the option to collecteach phase into different types of vessels

Drag and adjust %B
for quick adjustment
to the gradient phase– Enables customization of collection options based on
detector signals, conductivity, pH, and %B■■■

Chromatogram comparison and peak analysis
 dvanced integration parameters enable manual integrationAfunctions and peak addition or removal for fine-tuning data rganized data and easy navigation with intuitiveO
grouped table layouts■

Multi-instrument control■

Centralized database for easy data storage and sharing■■

Fraction heat maps to help locate protein of interest
 rotein quantitation is calculated upon entering extinctionP
coefficient

For More Information
Web: bio-rad.com/chromlab
Learn More about Multi-D Chromatography
Web: bio-rad.com/Multi-D

Ordering Information
Catalog #Description

12009390 hromLab Software and Documentation USB, single software platform compatible with all NGC systems,C
includes integrated system setup controls, method development, and data acquisition and analysis. Compatible
with Windows 7–10 64 bit

160 | Chromatography: Laboratory and Process Separations

Price, U.S. $2,500.00

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
bio-rad.com/NGC

ChromLab Software, User Management Edition
ChromLab Software, User Management Edition, enables
multiple system control from a single PC and the ability to save
all methods, runs, and analyses to a centralized database.
User management enables assignment of user permissions tocontrol read, write, and edit access to all data files.

■■

 hromLab administrator can designate a centralCcomputer as the host of a shared ChromLab database ata can be shared among ChromLab computers andD
NGC systems

Ordering Information
Catalog #Description

17000099
17000098
17000097

 hromLab User Management License, 1 seatC
ChromLab User Management License, 3 seatsChromLab User Management License, 5 seats

Price, U.S. $InquireInquireInquireChromLab Software, Security Edition
ChromLab software, Security Edition, is available with one,
three, or five licenses. When enabled, Security Edition
provides the necessary features to permit users to operate
in compliance with Title 21 of the U.S. Code of Federal
Regulations Part 11 (U.S. FDA 21 CFR Part 11) within aclosed system.

■■

Prevent falsification of data■

Generate binding signatures and records■

ChromLab software, Security Edition, provides tools to:■Limit and authorize system access to authorized users■

Produce computer-generated audit trails

 imit access to selected tasks through user levelLpermissions

 reate accurate and complete signed reports forC
U.S. Food and Drug Administration (FDA) submissions

Ordering Information
Catalog #Description

7886001 hromLab Security Edition (SE Software) USB, single software platform compatible with all NGC
C
systems, includes integrated system setup controls, method development, data acquisition, and analysis.
Security Edition also provides features in accordance with U.S. FDA 21 CFR Part 11 requirements. 1 licenseChromLab Security Edition (SE Software) USB, 3 licenses
ChromLab Security Edition (SE Software) USB, 5 licenses

7886003
7886005

Price, U.S. $7,000.00

InquireInquireChromLab Software, IQ/OQ
The NGC IQ/OQ is designed to evaluate the installationand operation of the instrument. This service is provided byour trained field service engineers utilizing the Installation
Qualification and Operational Qualification protocols wehave developed. Features and benefits of NGC installationand qualification and operational qualification:

■

■

■

■

Enable U.S. FDA 21 CFR Part 11 compliance
Ensure system performance
Are performed by trained field service engineers
Are performed at customer sites

For More Information
Web: bio-rad.com/NGCiqoq

Ordering Information
Catalog #Description

7880020IQOQ

 GC Quest/Quest Plus IQ/OQ (with fraction collector and air sensor), Installation Qualification andN
Operational Qualification (IQ/OQ) performed by field service engineers on location at the customer’s site,
duration: 1 day
NGC Scout/Discover/Discover Pro IQ/OQ (with fraction collector and air sensor), duration: 2–3 days

7880030IQOQ

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $6,999.00

InquireChromatography: Laboratory and Process Separations | 161

Medium-Pressure Chromatography Systems
Fraction Collectors

bio-rad.com/fractioncollectors

Fraction Collectors
NGC Fraction Collector
The NGC fraction collector’s (NGC FC) flexibility andversatility improves high-throughput purification workflows.
It can collect into multiple vessel types within a method
and has increased capacity compared to the BioFrac
fraction collector. The NGC chromatography systempaired with the NGC fraction collector provides collection
options for analytical to preparative chromatography
applications and is well suited to meet the purificationneeds of industry laboratories.
Key features:
■

■

■

■

 aximizes lab efficiency with automated collection intoM
multiple vessel types within a method, from 96-wellplates to 250 ml bottles
 nables production batch capacity by collecting up toE
80 unlimited volume fractions
 as double or more the capacity of the BioFrac fraction
Hcollector
 an connect 2 fraction collectors to the NGC
C
chromatography system — 1 NGC FC with 1 BioFrac FC
or 2 NGC FCs■

Supports flow rates up to 200 ml/min■

Has a sturdy design with a new range of metal racks

■

■

■

■

 omes factory calibrated so no need to calibrate forC
different rack types

Versatile Rack Options
Capacity Comparison between the BioFrac and NGC
Fraction Collectors
BioFracFraction Collector

Has multitrigger collection: slope, pH, conductivity, etc llows for faster downstream analysis with easy accessA
to fractions during a run
IEC/EN 61010, IEC/EN 61326, IEC/EN 61000, and
CAN/CSA 22.2 certification

Optional Components
Enclosure maintains sample integrity without limiting
access, protecting your samples from environmental
conditions while allowing full sample access during a run■■

NGC Fraction Collector

Prep rack adaptor for limitless volume sample collection

For More Information
Web: bio-rad.com/NGCFC
Download bulletin: 6963

162 | Chromatography: Laboratory and Process Separations

Ice bath

Cooling option
Vessel Type Size
12–13 mm15–16 mm
18–20 mm/15 ml
30 mm/50 ml
0.5 ml
1.5–2.0 ml microtubes
250 ml bottles
96-well plate48-well plate24-well plate
12-well platePrep-20 rack

Tubes/Racks

Capacity

90
60
40
6
63
42
4
4
4
4
4
20

180
120
80
24
252
168
4
4
4
4
4
20

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
Fraction Collectors

bio-rad.com/fractioncollectors

NGC Fraction Collector Enclosure
Optional enclosure protects fractions from dust andparticulates without restricting access to tubes and racks.
■

Unrestricted access to tubes and racks■

Protects fractions from dust and airborne particulates■

For More Information
Download document and bulletin:
10000062077 and 6963

 ightweight design with access to fraction collector fromL
front or sides

Ordering Information
Price, U.S. $Catalog #Description

17002070

NGC Fraction Collector, 100/240 V, includes two 13 mm racks, tubing kit,external power supply, and USB cable

Accessories
12009848
12003752
12003755
12003756
12003757
12003754
12003753
12003760
17002942
12005823
12005822

NGC Fraction Collector Enclosure
Rack for 13 mm Tubes, for 96 x 13 mm tubes
Rack for 16 mm Tubes, for 75 x 16 mm tubes
Rack for 18 mm/15 ml Tubes, for 75 x 18 mm tubes
Rack for 50 ml Tubes, for 27 x 29 mm tubes
Rack for Deep Well Microtiter Plate, for 2 x 24-, 48-, and 96-well plates
Rack for 1.5–2 ml Capless Microtubes, for 96 tubes
Plate Rack Riser
NGC Fraction Collector Rack Adaptor
Funnel Adaptors R/L Adaptor Kit
Dispense Tip Replacement Kit

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 163

Medium-Pressure Chromatography Systems
Fraction Collectors

bio-rad.com/fractioncollectors

BioFrac Fraction Collector
The easy-to-program BioFrac fraction collector can beused for basic or complex fraction collection schemes.
Off-the-shelf racks extend the versatility of collection
schemes and provide cost-effective storage of samples.
Off-the-shelf racks are autoclavable, easy to assemble,
and lie flat, using little storage spaceA

Key features include:■Collection in time or drop mode (or volume mode whenconnected to an NGC or chromatography system)■

Collection of peaks by peak detection, time windows (upto 20), or a combination of both■

Drop arm movement in column, row, or serpentine

pattern movements for microplates or Titertube tubes■

BA dispenser arm that is manually adjustable to tube

heights ≤150 mm■

Numerous off-the-shelf racks to accommodate tubes(12–20 mm diameter), Eppendorf or other microtubes(0.5, 1.5, or 2.0 ml), or scintillation vials■

Multirun feature that allows overlay of fractions■

IEC 6101A 22.2 certification

Optional Components
BioFrac ice bath/microplate rack with tube
grips that can hold 13 mm tubes■■

CBioFrac prep-20 preparative rack with up to 20 collection

ports for collection from bottles to carboys■

Microplate drophead kit for precise collection of small

volumes into microplates
For More Information
Web: bio-rad.com/biofrac
Download bulletin: 2711

D

Flexible rack options. The ice bath/microplate rack is used to collectfractions in chilled test tubes (A) or up to four microplates (B) (shownusing the optional 25 µl drophead). The prep adaptor rack (C) is used
for preparative fractionation into 1–20 collection vessels of any size.
The BioFrac fraction collector holds up to four H4-high racks (D).
The BioFrac fraction collector accepts a variety of off-the-shelf racks.

164 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Medium-Pressure Chromatography Systems
Fraction Collectors

bio-rad.com/fractioncollectors

Ordering Information
Catalog #Description

7410002

 ioFrac Fraction Collector, input voltage 100/240 V, includes power cord, rack set F1 (2 x flatpack, 13 mm),B
BioFrac diverter valve, fittings kit, and NGC communication adaptor

5,415.00

Rack Set F1, 2 x flatpack, with numbered tube positions, each holds 90 tubes, 12–13 mm diameter
Rack Set F2, 2 x flatpack, each holds 60 tubes, 15–16 mm diameter, for total of up to 120-tube collection
Rack Set F3, 2 x flatpack, each holds 40 tubes, 18–20 mm diameter, for total of up to 80-tube collection
Rack Set H1, 4 x flatpack, each holds 42 capless 1.5 ml Eppendorf or other microtubes for total of up to
168-microtube collection
Rack Set H2, 4 x flatpack, each holds 63 capless 0.5 ml Eppendorf or other microtubes for total of up to
252-microtube collection
Rack Set H3, 4 x flatpack, each holds 30 reduced-volume scintillation vials, 16 mm diameter,
for total of up to 120-vial collection
Rack Set H4, 4 x flatpack, each holds 6 scintillation vials, 30 mm diameter, for total of up to 24-vial collection
BioFrac H4-High Rack Set, 4 x flatpack, each holds 6 centrifuge tubes, 30 mm diameter,
for total of up to 24-vial collection
BioFrac Ice Bath/Microplate Rack, can hold 120 tubes, 12–13 mm diameter; up to 4 SBS-format
microplates in 96-, 48-, 24-, or 12-well configurations; Titertube microtube collection, 8 x 12,
96-tube configuration
BioFrac Prep-20 Preparative Rack, for fractionation into 1–20 collection vessels of any size
BioFrac Fraction Collector Fittings Kit, includes replacement fittings and tubing for setup of the fraction
collector to the NGC system
BioFrac Microplate Drophead Kit, includes preassembled drophead nut with 0.020" ID Tefzel tubing;delivers approximately 25 µl per drop
BioFrac Accessory Cable, 15-pin D to bare wires, for connecting BioFrac fraction collector to other equipment;
for input or output signals

145.00145.00145.00235.00Accessories
7410010741001174100127410013741001474100157410016741002074100177410018
741000774100887318290Tubes
22397502239751
2239500

Price, U.S. $Clear Polystyrene Tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000
Natural Polypropylene Tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000
Micro Test Tubes, capless, 1.5 ml, polypropylene, natural, graduated, 500

In the U.S., call toll free at 1-800-424-6723

235.00235.00235.00175.00265.00145.00175.00100.00185.00

51.00
51.00
24.00

Chromatography: Laboratory and Process Separations | 165

Chromatography Accessories
bio-rad.com/chromaccessories

Chromatography Accessories
Low-Pressure Tubing■

Silicone tubing — contains no cytotoxic extractables■

and has excellent wetting properties. Autoclavable andheatable for pyrogen removal. May be damaged byconcentrated acids and bases■

virtually any reagent, stable up to 400°C
Pump Tubing Kits
Precut tubing for the Model EP-1 Econo pump, Econo
gradient pump, and BioLogic LP system is available in
PharMed and silicone with a choice of 0.8, 1.6, or 3.2 mm
ID. Each kit contains 20 pieces of precut pump tubing andfour sets of luer lock fittings and tubing retainers.

Tygon tubing — clear and tough, tolerates greaterpressures than silicone; transparent; should not be
autoclaved; may be damaged by high concentrationsof alcohol■

PTFE tubing — chemically inert; can be used with

PharMed tubing — has a wider range of chemicalcompatibility than silicone or Tygon and is ideal for use
in pump heads of the Model EP-1 Econo pump, Econo
gradient pump, and BioLogic LP system; lasts 10 times
longer than Tygon or silicone

Tubing size (ID x wall)

Tubing Size Selection Chart
0.51 x 0.8 mm
0.8 x 0.8 mm
1.6 x 0.8 mm
3.2 x 0.8 mm
0.001 0.002

0.005

0.01

0.02

0.05

0.10

0.20

0.5

1.0

2

5

10

20

Flow rate, ml/min
Tubing size selection. Comparison of flow rate ranges of various tubing sizes (ID) when used in the Econo gradient pump,
the Model EP-1 Econo pump, and the BioLogic LP system.
Tubing Material Comparison
Appearance
Flexibility
Autoclavability
Chemical compatibility
Performance in peristaltic pumps

Silicone

Tygon

PharMedPTFETranslucent
Excellent
Yes
Fair
Good

Clear
Excellent
No
Fair
Fair

Off-white
Excellent
Yes
Good
Excellent

Translucent
Fair
Yes
Excellent
Not acceptable

166 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Accessories
bio-rad.com/chromaccessories

Ordering Information
Catalog #Description

Price, U.S. $Silicone Tubing
7318210
Silicone Tubing, 0.8 mm ID/0.8 mm wall, 10 m
7318211
Silicone Tubing, 1.6 mm ID/0.8 mm wall, 10 m
7318212
Silicone Tubing, 3.2 mm ID/0.8 mm wall, 10 m

81.00
89.00
100.00Tygon Tubing
731821373182147318215

48.00
54.00
54.00

Tygon Tubing, 0.51 mm ID/0.8 mm wall, 10 m
Tygon Tubing, 0.8 mm ID/0.8 mm wall, 10 m
Tygon Tubing, 1.6 mm ID/0.8 mm wall, 10 m

PharMed Tubing
7318207
PharMed Tubing, 0.8 mm ID/1.0 mm wall, 10 m
7318208
PharMed Tubing, 1.6 mm ID/1.0 mm wall, 10 m
7318209
PharMed Tubing, 3.2 mm ID/1.0 mm wall, 10 m

95.00
92.00
100.00

Pump Tubing Kits
7318240
Pump Tubing Kit, 0.8 mm ID silicone, 20 precut lengths and 4 sets of fittings, for use with EP-1 Econo pump
7318241
Pump Tubing Kit, 1.6 mm ID silicone, 20 precut lengths and 4 sets of fittings, for use with EP-1 Econo pump
7318242
Pump Tubing Kit, 3.2 mm ID silicone, 20 precut lengths and 4 sets of fittings, for use with EP-1 Econo pump
7318247
Pump Tubing Kit, 0.8 mm ID PharMed, 20 precut lengths and 4 sets of fittings
7318248
Pump Tubing Kit, 1.6 mm ID PharMed, 20 precut lengths and 4 sets of fittings
7318249
Pump Tubing Kit, 3.2 mm ID PharMed, 20 precut lengths and 4 sets of fittings
7319007
Econo Gradient Pump Tubing Kit, includes 2 each of 0.8, 1.6, and 3.2 mm PharMed tubing, for use with
Econo gradient pump

93.00
93.00
99.00
110.0099.00
115.0092.00

High-Pressure Tubing and Tubing Kits■

chemically inert and autoclavable. Tefzel is translucent
and slightly flexible. PEEK is opaque; the color indicatesthe inside diameter

PTFE FEP tubing — recommended for pre-pumpconnections; can withstand medium pressure; translucent,semiflexible, chemically inert, and autoclavable■Tefzel or PEEK tubing — recommended for post-pumpconnections; both withstand high pressure and are■Tubing kits — set of premade tubing with fittings to

simplify setup of the BioLogic DuoFLow system

Ordering Information
Catalog #Description

7500603

 TFE FEP Tubing, 1/8" (0.125", 3.2 mm) OD x 0.062" (1.6 mm) ID, 15' (4.6 m),
P
for pre-pump buffer inlet lines to the pump heads
Tefzel Tubing, 1/16" (0.062", 1.6 mm) OD x 0.020" (0.5 mm) ID, 30' (9.1 m),
for system connections post-pump
PEEK Tubing, orange, 1/16" OD x 0.020" ID x 30', rated to 5,000 psi
PEEK Tubing, green, 1/16" OD x 0.030" ID x 30', rated to 3,000 psi
F10 Tubing Kit, includes precut and fitted PTFE, Tefzel, and orange PEEK tubing for installation of
BioLogic DuoFlow basic chromatography system running at flow rates <40 ml/min
F40 Tubing Kit, includes precut and fitted PTFE, Tefzel, and green PEEK tubing for installation of
BioLogic DuoFlow basic chromatography system running at flow rates ≥40 ml/min

7500602
7600604
7600605
7600650
7600652

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $69.00
150.00390.00140.00405.00390.00Chromatography: Laboratory and Process Separations | 167

Chromatography Accessories
bio-rad.com/chromaccessories

Medium- and High-Pressure Fittings
The NGC fittings kit includes all parts necessary to connect medium- and high-pressure columns to medium-pressure
chromatography systems.
For More Information Download bulletin: column connection instructions — 5326
Ordering Information
Catalog #DiagramDescription

Quantity

Medium- and High-Pressure Fittings Kits
7600550
BioLogic DuoFlow Kit, includes PEEK and Tefzel nuts, ferrules, unions, plugs,
and luer syringe
7884017
NGC Fittings Kit, Includes PEEK and Tefzel nuts, ferrules, unions, plugs, tubing
cutter, fittings tightener, and luer syringe, for use with NGC systems

1

Price, U.S. $535.001

Individual Medium- and High-Pressure Fittings
7885015

PEEK Nut, 1/8", 10

10

4.00

7885007

10-32 PEEK Union, 1/16", 0.020

1

58.00

7885008

Fittings Tightener, short

1

5.00

1

175.001

59.00

78850107320113Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting
to connect male end of luer column to NGC system
Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings
kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1
cartridge to BioLogic DuoFlow and NGC systems

7500556

Ferrule and Lock Ring, for 1/16" OD (1.6 mm) tubing

10

67.00

7500559

Tefzel Cap, 1/4–28 female connection, to plug unused tubing

5

35.00

7500560

BioLogic Fittings Tool

1

14.00

7500561

Tefzel Union Adaptor, 1/4–28 to M6, to connect 1 M6 column to a
BioLogic DuoFlow system

2

26.00

7500562

Tefzel Union, 1/4–28 to 1/4–28 to extend tubing

5

51.00

7500563Tefzel Plug, 1/4–28 male connection, to plug unused ports on valves and columns

5

23.00

2

60.00

1 set

60.00

1 set

78.00

1 set

36.00

1 set

47.00

7500564
7500565
7500566
75005677500568 itting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC
F
column (10-32) to BioLogic DuoFlow and NGC systems
Econo-Column to BioLogic System Fittings Kit, 2 fittings to connect
1 Econo-Column (luer) column to a BioLogic DuoFlow system
Bottle Cap Kit, includes 2 bottle caps, 2 plugs
 NO M6 Fittings Kit, includes 2 nuts and 4 ferrules to connectU
UNO column to an FPLC system
UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect
UNO column to an HPLC system, for use with BioLogic DuoFlow
and NGC systems

7500569

Delrin Nut, for 1/16" OD (1.6 mm) tubing

10

16.00

7500570

Delrin Nut, for 1/8" OD (3.2 mm) tubing

5

13.00

7500571

Ferrule and Lock Ring, for 1/8" OD (3.2 mm) tubing

5

30.00

7500703

Inline Filter Kit, includes 1 inline filter and 2 replacement frits

1

94.00

7500704

Replacement Frits, for inline filter kit

5

30.00

7500553

1/8" OD (3.2 mm) Pre-Pump Fittings, includes Delrin nut, ferrules, lock ring

5

78.00

7500554

1/16" OD (1.6 mm) Post-Pump Fittings, includes Delrin nut, ferrules, lock ring10

185.007601308

Long Fingertight Fittings, 10-32 x 0.82", for PEEK and Tefzel tubing

4

41.00

7601311

Long Fingertight Fittings, 10-32 x 1.03", for PEEK and Tefzel tubing

4

51.00

168 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography Accessories
bio-rad.com/chromaccessories

Low-Pressure Fittings
The low-pressure system fittings kit is useful to adapt various tubing sizes, to make liquid connections,and to direct and stop liquid flow.
For More Information
Download bulletins: column connection instructions — 5326, 6456
Ordering Information
Catalog #Description

Low-Pressure Fittings Kit
73182207319006

Low-Pressure System Fittings Kit, polycarbonate/polypropylene, 250 pieces
Econo Gradient Pump Fittings Kit, includes 32 fittings, 12 tubing retainers

Catalog #Description

DiagramPrice, U.S. $Quantity

Material

340.00100.00Price, U.S. $Individual Low-Pressure Fittings
7318221*0.8 mm Barb to Female Luer

25

Polypropylene

41.00

7318222*

1.6 mm Barb to Female Luer

25

Polypropylene

43.00

7318223*

3.2 mm Barb to Female Luer

25

Polypropylene

43.00

7318224

0.8 mm Barb to Male Luer

25

Polypropylene

43.00

7318225

1.6 mm Barb to Male Luer

25

Polypropylene

43.00

7318226

3.2 mm Barb to Male Luer

25

Polypropylene

43.00

7318228

Female Luer to Female Luer

10

Polypropylene

43.00

7318230

Male Luer to Male Luer

10

Polypropylene

37.00

7318232

Female Luer Plug

25

Polypropylene

37.00

7318233

Male Luer Plug

25

Polypropylene

37.00

7318229

Female Luer T-Connector

10

Polypropylene

37.00

7328300

 .8 mm Barb to Barb Connector, recommended for0minimal dead-volume connection

25

Polypropylene

46.00

73281033-Way Stopcock, 2 female luer to male luer

10

Polycarbonate/polypropylene

57.00

7328102

2-Way Stopcock, female luer to male luer

10

Polycarbonate/polypropylene

55.00

7323245

Luer Tubing Adaptor, with 5' of 0.8 mm ID PTFE tubing

5

Polypropylene/PTFE

240.001

Polypropylene/PTFE

96.00

1

PEEK/Tefzel

145.001

Polypropylene/PTFE

97.00

1

Polypropylene/PTFE

59.00

7328202732011173201127320113

 ouble Luer Tubing Adaptor, with 5' of 0.8 mm
D
ID PTFE tubing
Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings
to connect 1 cartridge to an FPLC system
Luer to 10-32 Adaptor Fittings Kit, includes luer to
10-32 fittings to connect 1 cartridge to an HPLC system
or NGC system
Luer to BioLogic and NGC Systems Fittings Kit, lowpressure system fittings kit, includes 1/4–28 female to maleand 1/4–28 female to female luers to connect 1 cartridge to
BioLogic DuoFlow and NGC systems* Fits inlet and outlet of Econo-Column chromatography columns and low-pressure tubing.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 169

Low-Pressure Chromatography Systems
bio-rad.com/lowpressure

Model EM-1 Econo UV Monitor
The Model EM-1 Econo UV monitor is a single-wavelength
detector for flow-through monitoring of effluents fromchromatographic columns, centrifugation gradients, andother devices. The monitor consists of a control unit andan optics module that includes both 254 and 280 nm filters
and a 2 mm pathlength flow cell.
■ ortable optics module with detection close to the columnP
outlet to maximize ­resolution■

Autozero functionality■

Coldroom compatibility■

LED display of absorbanceSpecifications
Wavelength
254 and 280 nm
Sensitivity ranges
2.0, 1.0, 0.5, 0.2, 0.1, 0.05, 0.02, 0.01 AUFS
Detection limit	7 µg/ml (BSA in H2O)
Lamp
Low-pressure mercury with ­phosphor screen
Filters
254 and 280 nm
0–1 V analog (impedance 150 W)
Output signal
4–40°COperating temperature
Optical path 2 mm, internal volume 80 µl,
Flow cell		
illuminated volume 3 µl

Noise
		
		
Safety		
Dimensions (W x D x H)
		

1.0 x 10 –4 OD max. peak-to-peak(dry cell); 2.0 x 10 –4 OD max.
peak-to-peak (flowing liquid)
Meets IEC 61010 and CSA
22.2 certification
Base unit: 14.6 x 18.6 x 20.2 cm
Optics unit: 13.2 x 15.2 x 3.8 cm

For More Information
Web: bio-rad.com/modelem1

Ordering Information
Catalog #Description

7318160
7318162 odel EM-1 Econo UV Monitor, 100/120 V, includes control module, optics module, filters for 254 andM
280 nm wavelengths, system cable 4, fittings kit
Model EM-1 Econo UV Monitor, 220/240 V

Price, U.S. $Accessories
73181657318166
7318167

UV Flow Cell, replacement
Lamp, replacement
Filter Assembly, 254 and 280 nm

345.00350.00460.00170 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

3,690.00
3,815.00

Process-Scale Separations
bio-rad.com/process

Process-Scale Separations
Bio-Rad has extensive experience serving the separation technology community with process resins that offer
optimal solutions for your separation needs. To order Bio-Rad process-scale resins, contact your local Bio-Rad
sales representative.
Process Resin Selection Guide

Resin

Available inProcess-Scale Bulk Resin
Page #

Prepacked Columns
and Plates
Page #

Prepacked ProcessScale Columns
Page #

Affinity 				Nuvia IMAC
106
174
Nuvia IMAC, uncharged
106
127
108
Profinity IMAC
127
Affi-Prep Protein A
112
127
Affi-Gel 10/15
116		
Affi-Gel Blue
113
127
DEAE Affi-Gel Blue
113
127
CM Affi-Gel Blue114		
Affi-Prep polymyxin
115		
Affi-Gel boronate
114		
Affi-Gel HZ
116		
Affi-Gel 102
116		
Analytical Grade Resins			
AG 1, 4
94–96		AG 50W
94–96		AG 501
94–96		Chelex
94–96		Hydrophobic Interaction			
Macro-Prep Methyl HIC
119–120128
Macro-Prep t-Butyl HIC
119–120
128
Bio-Beads SM-2
120		
Ion Exchange 			Nuvia Q
88–90
127, 174
Nuvia S
88–90
127, 174
Nuvia HP-Q
88–90
127, 174
88–90
127, 174
Nuvia HR-S
UNOsphere Q
88–90
127, 174
UNOsphere S
88–90
127, 174
Macro-Prep High Q
91–93
127
Macro-Prep DEAE
91–93
127
Macro-Prep High S
91–93
127
Macro-Prep CM
91–93
127, 174
Mixed-Mode			Nuvia aPrime 4A
99–100128, 174
Nuvia cPrime
99–100128, 174
CHT Type I
101–103, 176
127, 174
129, 173
CHT Type II
101–103, 176
127, 174
129, 173
CHT XT
101–103, 176
127, 174
129, 173
MPC
104127, 174
Bio-Gel HT/HTP
105
104
CFT Type II
127
Size Exclusion 			
Bio-Gel A
118		
127
Bio-Gel P
117
Bio-Beads SM-2
120
119		
Bio-Beads S-X
For product applications and descriptions, please refer to the chromatography media selection guide on pages 85–88.

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 171

Process-Scale Separations
bio-rad.com/process

Process-Scale Chromatography Resins
the purification of biomolecules. Macro-Prep resins are an
excellent choice for process-scale applications such as
blood fractionation purification.
For More Information
Web: bio-rad.com/processIEX

Nuvia resins — a family of next-generation ion-exchange
and metal affinity products built on an industry proven rigid
polymer base matrix. Nuvia Q and S resins offer superior
flow properties and low nonspecific binding while deliveringhigh capacity and unique selectivity. Nuvia HR-S is a highresolution cation exchanger capable of separating very
challenging high molecular weight impurities. Nuvia HP-Q isa strong anion exchange resin designed for high-throughput
purification of large biomolecules, such as IgA, IgM, fibrinogen,factor VIII, von Willebrand factor, and polyethylene glycol–
modified proteins. Nuvia IMAC is available in two forms:
uncharged and charged with Ni2+. The uncharged form canbe charged with the metal ion of your choice for even greater
purification flexibility. Nuvia IMAC is a high-capacity metalchelate affinity resin. It has optimized particle size and highporosity that allow operation at higher flow rates withoutcompromising binding capacity. Nuvia resins bring together
a unique set of properties specifically designed to meet thedemands of current and future downstream processes. Nuvia
resins are flexible and robust with a large operational window,
making them effective in capture and/or polish steps.
For More Information
Web: bio-rad.com/nuvia

UNOsphere resins — specialized for fast mass transfer,
UNOsphere resins deliver high binding capacity at high flow
rates while maintaining low backpressure. These robust
polymeric resins formed by single-step polymerization carryeither anionic (UNOsphere Q) or cationic (UNOsphere S)
functionality, making them ideal for the efficient purification ofbiopharmaceutical molecules from feedstream at any stageof the downstream process.
For More Information
Web: bio-rad.com/processIEX

Macro-Prep resins — polymeric methacrylate resins areavailable with strong or weak ion exchange and hydrophobic(methyl and t-butyl) functionalities. These rigid macroporoushydrophilic resins provide excellent dynamic binding
capacity, resolution, and throughput at high flow rates for

172 | Chromatography: Laboratory and Process Separations

Ceramic hydroxyapatite — CHT is a robust mixed-mode
media with unique separation properties that delivers
exceptional selectivity for purification of biomolecules.
As a proven scalable polishing step in mAb and vaccine
purification, CHT effectively eliminates common feedstream
contaminants, such as aggregates, leached Protein A,
DNA, and host cell proteins, in a single step. This provides
you the flexibility to maximize your process economics.
Bio-Rad now also offers CHT XT, engineered withadvanced technology, resulting in unique selectivity androbust, uniformly-sized beads with excellent pressure-flow
properties.
For More Information
Web: bio-rad.com/processCHT

Ceramic fluoroapatites — MPC is a fluorinated derivativeof ceramic hydroxyapatite providing the same robust
impurity clearance as CHT. Use MPC media for specialty
protein purification applications. CFT is a chemically pureform of fluoroapatite. It is a rigid, spherical, and macroporous
media used in the purification of biologically significant
compounds and is ideally suited for the bioprocessing
industry. CFT can be used under stringent conditions toseparate acidic proteins requiring buffered conditions as low
as pH 5.6. CFT has high binding capacity and may be used
reproducibly over an extended number of chromatography
runs. When CFT is used, process engineers can perform
purifications across a range of lower pH values to obtain
optimal and reproducible results for the targeted biomolecule.
For More Information
Web: bio-rad.com/MPC
bio-rad.com/processCFT

Nuvia mixed-mode resins — Nuvia aPrime 4A and Nuvia
cPrime resins are designed for process-scale purification ofa wide variety of therapeutic proteins. The unique selectivity
allows method developers to use hydrophobic and cationexchange modes (cPrime) and hydrophobic and anion
exchange modes (aPrime) to achieve effective purification.
The resins have a large design space for binding and elution,
allowing for the development of highly robust methods in acommercial manufacturing setting. Nuvia resins are built on arigid, mechanically and chemically stable macroporous basematrix with a particle size optimized to deliver exceptional
flow properties, fast mass transfer, and stability.
For More Information
Web: bio-rad.com/mixedmode

In the U.S., call toll free at 1-800-424-6723

Process-Scale Separations
bio-rad.com/process

AG resins — AG resins are a highly referenced andextensively used line of chromatography resins for the
separation of low molecular weight molecules, such as
inorganic ions, organic acids, peptides, and carbohydrates,from biopharmaceutical preparations and ancillary buffers
used in biomanufacturing. They are highly processed toremove impurities. Biotechnology-grade AG resins arefurther treated to reduce bioburden to extremely low levels,
making them suitable for process-scale purification ofbiopharmaceuticals.
For More Information
Web: bio-rad.com/processAG

Bio-Beads SM-2 resin — nonpolar polystyrene-based
resin for hydrophobic interaction chromatography,
Bio-Beads are used extensively for the removal of nonpolar
detergents from biological preparations for manufacturing
at both laboratory and process scale. The resin is reusable
and can be easily cleaned with alcohol solutions followed bya distilled water rinse.
For More Information
Web: bio-rad.com/processHIC

Visit bio-rad.com/samplenow to request samples ofthese resins.

Chelex resin — these unique chelating resins bind polyvalentcations with high selectivity and are used to remove metal
ions from samples and buffers. They are extensively usedin environmental applications, such as glyphosate isolation.
Chelex resins are made from a styrene divinylbenzene
support coupled to paired iminodiacetate ions.
For More Information
Web: bio-rad.com/processAG

Chromatography Screening Tools and Prepacked Columns
Foresight Prepacked Plates and Columns
Foresight plates and columns are prepacked with a rangeof Bio-Rad’s process chromatography resins, offering
process scientists convenience and reliability for their highthroughput experimentation needs. Their robust design
allows process scientists to use the prepacked formats
through the entire purification development cycle fromhigh-throughput media screening and small-scale method
development to scale-up optimization.
■

■

■

■

■

 repacked and ready-to-use formats are designed toPsave process development time ifferent experimental conditions can be evaluated toDbetter define an operational window
 igh-throughput experiments with minimal sample
H
requirements can be performed
 vailable in a variety of chromatography resin modesA
designed for large-scale bioprocess
Compatible with robotic liquid handling workstations

For More Information
Web: bio-rad.com/foresightIn the U.S., call toll free at 1-800-424-6723

Chromatography Media Available in Foresight Formats
Chromatography Resin

Mode

UNOsphere Q
Strong anion
UNOsphere S
Strong cation
Nuvia Q
Strong anion
Nuvia S
Strong cation
Nuvia HR-S	Strong cation
Nuvia HP-Q
Strong anion
Nuvia cPrime	Mixed-mode hydrophobic: cationicNuvia aPrime 4A
Mixed-mode hydrophobic: anionic
MPC Type I — 40 µm particle size
Mixed-mode metal affinity: cationic
CHT Type I — 40 µm particle size	Mixed-mode metal affinity: cationic
CHT Type II — 40 µm particle size	Mixed-mode metal affinity: cationic
CHT XT
Mixed-mode metal affinity: cationicNuvia IMAC
Metal affinity

Chromatography: Laboratory and Process Separations | 173

Process-Scale Separations
bio-rad.com/process

Videos
Product
Demonstration

bio-rad.com/
CHTpacking

Product
Demonstration

bio-rad.com/
ProcessIMAC

Ordering Information
Catalog #Description

Price, U.S. $Foresight Plates, 2 x 96-Well
732-4714
Foresight UNOsphere Q, 20 µl
732-4710
Foresight UNOsphere S, 20 µl
732-4703
Foresight Nuvia Q, 20 µl
732-4701
Foresight Nuvia S, 20 µl
732-4707
Foresight Nuvia HR-S, 20 µl
12006908
Foresight Nuvia HP-Q, 20 µl
732-4705
Foresight Nuvia cPrime, 20 µl
732-4716
Foresight CHT Type I, 40 µm, 20 µl
732-4718
Foresight CHT Type II, 40 µm, 20 µl
732-4785
Foresight MPC Type I, 40 µm, 20 µl
12004035
Foresight Nuvia IMAC Ni-Charged, 20 µl
12003151
Foresight CHT XT Plates, 40 μm, 20 μl
12007411
Foresight Nuvia aPrime 4A Plates, 20 μl
Description
Foresight Columns
Foresight UNOsphere Q
Foresight UNOsphere S
Foresight Nuvia Q
Foresight Nuvia S
Foresight Nuvia HR-S
Foresight Nuvia HP-Q
Foresight Nuvia cPrime
Foresight CHT Type I, 40 µm
Foresight CHT Type II, 40 µm
Foresight MPC Type I, 40 µm
Foresight Nuvia IMAC Ni-Charged
Foresight Nuvia aPrime 4A Column
Foresight CHT XT Column, 40 μm
Description
Foresight RoboColumn Units, 1 row of 8 columns*
Foresight UNOsphere Q
Foresight UNOsphere S
Foresight Nuvia Q
Foresight Nuvia S
Foresight Nuvia HR-S
Foresight Nuvia HP-Q
Foresight Nuvia cPrime
Foresight CHT Type I, 40 µm
Foresight CHT Type II, 40 µm
Foresight MPC Type I, 40 µm
Foresight Nuvia IMAC Ni-Charged
Foresight Nuvia aPrime 4A RoboColumn Unit
Foresight CHT XT RoboColumn Units, 40 μm

500.00500.00500.00500.00500.00500.00500.00500.00500.00500.00

1 ml

Price, U.S. $5 ml732-4732
732-4730
732-4721
732-4720
732-4723
12007020
732-4722
732-4735
732-4736
732-4737
12004038
12007392
12003150

104.25104.25115.83115.83126.79732-4752
732-4750
732-4741
732-4740
732-4743
12007021
732-4742
732-4755
732-4756
732-4757
12004037
12007393
12003149

200 µlPrice, U.S. $600 µl732-4819
732-4813
732-4804
732-4801
732-4831
12007013
732-4807
732-4822732-4825732-4828
12004051
12007394
12003152

175.00175.00180.00180.00185.00732-4820
732-4814
732-4805
732-4802732-4832
12007014
732-4808
732-4823
732-4826732-4829
12004052
12007395
12003148

141.95148.71148.71148.71104.25182.00185.00185.00Price, U.S. $192.14
192.14213.49213.49231.53278.22291.46291.46291.46
192.14210.00210.00225.00225.00235.00233.00233.00240.00240.00* Foresight RoboColumn units are to be used with robotic liquid handling workstations.
Please visit bio-rad.com/foresight for more information on prepacked columns and plates. For additional prepacked formats see
EconoFit Chromatography Columns on page 127.

174 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Process-Scale Separations
Process-Scale Prepacked Columns

bio-rad.com/ForesightProColumn

New Process-Scale Prepacked Chromatography Columns — GMP Ready

Foresight Pro Columns

Foresight Pro Chromatography Columns,
CHT Ceramic Hydoxyapatite
Bio-Rad offers Foresight Pro columns prepacked withCHT Ceramic Hydroxyapatite media. These are validated,
GMP ready columns specifically designed to be used forall biopharmaceutical manufacturing applications. The
columns have a sanitary design with minimal dead spaceand effective cleanability, and connections to pumping
systems are made via standard connections. CHT
prepacked columns are available in a range of column
diameters and bed heights. CHT prepacked columns areshipped in 0.1 N NaOH.
Foresight Pro column dimensions available with CHT
Ceramic Hydroxyapatite Type I, CHT Type II, and CHT XT
media (40 μm particle size).
Vessel Type Size
5
5
8
8
10
10
13
13
20
20
24
24
33
33
ID, inner diameter.

Tubes/Racks

Capacity

10
20
10
20
10
20
10
20
10
20
10
20
10
20

0.2
0.4
0.5
1
0.8
1.5
1.3
2.7
3.1
6.3
4.5
9
8.5
17

In the U.S., call toll free at 1-800-424-6723

■

■

■

■

■

■

■

■

■

GMP ready columns — validated and ready to use xcellent performance — data include asymmetry, HETP,
E
endotoxin, and bioburden
Robust column design ensures quality packing
 olumns maintain performance criteria after shippingCand cycling
Validated through ISTA 2A transportation testing ll wetted parts meet USP Class VI requirements
Aof USP <88>
Standard Tri-Clamp connections — plug and play olumns are packed in a controlled ISO Class 7
Ccleanroom ange of column diameters and bed heights —R
5–33 cm ID and 10 or 20 cm bed height■

Resin reference sample included with each column■

Regulatory support file available

For More Information
Web: bio-rad.com/ForesightProColumn
User guide: bio-rad.com/ForesightProGuide

Chromatography: Laboratory and Process Separations | 175

Process-Scale Separations
Process-Scale Prepacked Columns

bio-rad.com/ForesightProColumn

Ordering Information
Catalog #Description

Foresight Pro Column, CHT Type I, 40 μm
12014659
Foresight Pro Column, CHT Type I, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014660
Foresight Pro Column, CHT Type I, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014671
Foresight Pro Column, CHT Type I, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014672
Foresight Pro Column, CHT Type I, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014673
Foresight Pro Column, CHT Type I, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014674
Foresight Pro Column, CHT Type I, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014675
Foresight Pro Column, CHT Type I, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014676
Foresight Pro Column, CHT Type I, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014677
Foresight Pro Column, CHT Type I, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014678
Foresight Pro Column, CHT Type I, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014679
Foresight Pro Column, CHT Type I, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014680
Foresight Pro Column, CHT Type I, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014681
Foresight Pro Column, CHT Type I, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014682
Foresight Pro Column, CHT Type I, 40 μm, 17 L, 33 cm ID x 20 cm length
Foresight Pro Column, CHT Type II, 40 μm
12014697
Foresight Pro Column, CHT Type II, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014698
Foresight Pro Column, CHT Type II, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014699
Foresight Pro Column, CHT Type II, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014700
Foresight Pro Column, CHT Type II, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014701
Foresight Pro Column, CHT Type II, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014702
Foresight Pro Column, CHT Type II, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014703
Foresight Pro Column, CHT Type II, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014704
Foresight Pro Column, CHT Type II, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014705
Foresight Pro Column, CHT Type II, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014706
Foresight Pro Column, CHT Type II, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014707
Foresight Pro Column, CHT Type II, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014708
Foresight Pro Column, CHT Type II, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014709
Foresight Pro Column, CHT Type II, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014710
Foresight Pro Column, CHT Type II, 40 μm, 17 L, 33 cm ID x 20 cm length
Foresight Pro Column, CHT XT, 40 μm
12014725
Foresight Pro Column, CHT XT, 40 μm, 0.2 L, 5 cm ID x 10 cm length
12014726
Foresight Pro Column, CHT XT, 40 μm, 0.4 L, 5 cm ID x 20 cm length
12014727
Foresight Pro Column, CHT XT, 40 μm, 0.5 L, 8 cm ID x 10 cm length
12014728
Foresight Pro Column, CHT XT, 40 μm, 1.0 L, 8 cm ID x 20 cm length
12014729
Foresight Pro Column, CHT XT, 40 μm, 0.8 L, 10 cm ID x 10 cm length
12014730
Foresight Pro Column, CHT XT, 40 μm, 1.5 L, 10 cm ID x 20 cm length
12014731
Foresight Pro Column, CHT XT, 40 μm, 1.3 L, 13 cm ID x 10 cm length
12014732
Foresight Pro Column, CHT XT, 40 μm, 2.7 L, 13 cm ID x 20 cm length
12014733
Foresight Pro Column, CHT XT, 40 μm, 3.1 L, 20 cm ID x 10 cm length
12014734
Foresight Pro Column, CHT XT, 40 μm, 6.3 L, 20 cm ID x 20 cm length
12014735
Foresight Pro Column, CHT XT, 40 μm, 4.5 L, 24 cm ID x 10 cm length
12014736
Foresight Pro Column, CHT XT, 40 μm, 9.0 L, 24 cm ID x 20 cm length
12014737
Foresight Pro Column, CHT XT, 40 μm, 8.5 L, 33 cm ID x 10 cm length
12014738
Foresight Pro Column, CHT XT, 40 μm, 17 L, 33 cm ID x 20 cm length176 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations
Subject Index

Subject Index
Affi-Gel 10 and Affi-Gel 15 Resins, 116

Low-Pressure Tubing, 166

Affi-Gel and Affi-Prep Protein A Resins, 112

Macro-Prep HIC Resins, 119

Affi-Gel Blue Resin, 113

Macro-Prep Ion Exchange Media, 91

Affi-Gel Boronate Resin, 114

Medium- and High-Pressure Fittings, 168

Affi-Gel Heparin Resin, 115

Mini Bio-Spin Columns, 135

Affi-Gel Hz Hydrazide Resin, 116

Model EM-1 Econo UV Monitor, 170

Affi-Prep Polymyxin Resin, 115

Molecular Biology and Biotechnology Grade Resins, 98

AG, Bio-Rex, and Chelex Resins, 94

NGC Fraction Collector, 162

Aminex HPLC Columns, 137

NGC Fraction Collector Enclosure, 163

Bio-Beads SM-2 Adsorbents, 120

NGC Medium-Pressure Chromatography Systems, 150

Bio-Beads S-X Resins, 119

Nuvia IMAC Resin, 106

BioFrac Fraction Collector, 164

Nuvia Mixed-Mode Resins, 99

Bio-Gel A 1.5 m Resin, 118

Poly-Prep Columns, 142

Bio-Gel Hydroxyapatite HT and HTP, 105

Poly-Prep Ion Exchange Columns, 135

Bio-Gel P Resins, 117

Process-Scale Chromatography Resins, 172

Bio-Scale CHT Type I Columns, 136

Process-Scale Prepacked Chromatography Columns —GMP Ready, 132, 175

Bio-Scale MT High-Resolution Columns, 149
Bio-Spin and Micro Bio-Spin Columns, 134
Bio-Spin, Micro Bio-Spin, and Mini Bio-Spin Columns, 140
Carbodiimide Activated Resin, 116
Ceramic Fluoroapatites, 104
Ceramic Hydroxyapatites, 101
Chromatography Standards, 122
ChromLab Software, 159
ChromLab Software, IQ/OQ, 161

Profinity Epoxide Resin, 109
Profinity eXact Resin, 110
Profinity GST Columns and Reagents, 109
Profinity IMAC Resin, 108
Technical and Reactor Grade Resins, 97
UNO Monolith Ion Exchange Columns, 136
UNOsphere and Nuvia Ion Exchange Media, 88
UNOsphere SUPrA rProtein A Media, 111

ChromLab Software, Security Edition, 161
ChromLab Software, User Management Edition, 161
CM Affi-Gel Blue Resin, 114
DEAE Affi-Gel Blue Resin, 113
Econo Alpha Empty Glass Chromatography Columns, 147
Econo-Column Flow Adaptors, 146
Econo-Column Funnel, 147
EconoFit Chromatography Columns, 127
EconoFit Screening and Application Packs, 121
Econo-Pac Affinity and Desalting Columns, 139
Econo-Pac Columns, 141
ENrich High-Resolution Ion Exchange Columns, 125
ENrich High-Resolution Size Exclusion Columns, 126
Foresight Prepacked Plates and Columns, 173
Foresight Prepacked Screening Tools: Plates and Columns, 130
Glass Econo-Column Columns, 143
Glass Econo-Column Reservoirs, 147
High-Pressure Tubing and Tubing Kits, 167
Low-Pressure Fittings, 169

In the U.S., call toll free at 1-800-424-6723

Chromatography: Laboratory and Process Separations | 177

178 | Chromatography: Laboratory and Process Separations

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting
Electrophoresis and Blotting Solutions

180

Protein Electrophoresis
Power Supplies
Protein Standards
Mini-Format 1-D Electrophoresis Systems
Midi-Format 1-D Electrophoresis Systems
Large-Format Vertical Electrophoresis Systems
Buffers and Reagents for Protein Electrophoresis
Protein Stains
2-D Electrophoresis
Preparative Electrophoresis

180
183
187
192
201
209
214
221
224
229

Western Blotting
Stain-Free Western Blotting Protocol
Transfer Devices
Membranes and Filter Papers
Blotting Buffers and Blocking Reagents
Immunodetection Reagents and Kits

232
232
234
242
245
247

Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems
Pulsed Field Gel Electrophoresis
Buffers and Reagents for Nucleic Acid Electrophoresis
DNA Ladders

254
254
261
264
267

Electrophoresis and Blotting Solutions
bio-rad.com/proteinelectroblot

Electrophoresis and Blotting Solutions
Bio-Rad offers a complete solution for all of your electrophoresis and blotting needs, from sample preparation to
imaging. Built on over 50 years of pioneering expertise, our innovative products offer reliable, reproducible results forall of your applications.

Protein Electrophoresis
A complete suite of electrophoresis products, from cells and precast gels to buffers and reagents, is available for the
separation of proteins.
Learn More About the Technology
Web: bio-rad.com/tech/proteinelectro

Buffers &Reagents

page 214Standards

page 187

SamplePreparation

Electrophoresis
Systems & Gels

Preparative
Electrophoresis

Blotting

Detection

Imaging

Power
Supplies

page 229

page 183
Wet/Tank
Systems

page 237
Midi-Format
Systems

Mini-Format
Systems

page 192

page 201

Gel Drying
Solution

page 220180 | Electrophoresis and Blotting

Semi-Dry
Systems

page 235

Large-Format
Systems

page 209

Conjugates
andReagents

page 252

Stainspages 221, 253

Membranes

page 242

Chemiluminescence
Detection

page 248

Colorimetric
Detection

page 249

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
bio-rad.com/proteinelectroblot

Overview of Vertical Gel Electrophoresis Systems
The Mini-PROTEAN, Criterion, PROTEAN II, and PROTEAN Plus systems all consist of electrophoresis cells and blotting
equipment. These systems provide:■A choice of four size formats with options to meet your specific needs for resolution, capacity, and processing speed■

Cell designs that eliminate current leakage to provide the most reproducible gels and consistent run times■

Dodeca cells for high-throughput 2-D separations in mini-, midi-, and large-format gel sizes

Vertical Electrophoresis System Selection Guide		Mini-PROTEAN System

Criterion System (Midi)

PROTEAN II System (Large)

PROTEAN Plus System (Large)

Run 1–4 precast or handcast
gels in mini format with theMini-PROTEAN Tetra cell

Fast setup with drop-in gel andcell design (precast or handcast)
Run 1–2 precast Criterion or
handcast gels with the Criterion cell

Large-format gel system offers
greater resolution over smaller
formats and can be used withhandcast gels

Offers maximum resolution in asingle gel and the longest range ofmolecular weight (MW) separation

Integrated upper buffer chamber
ensures leak-free operation

Versatility to perform 1-D or
2-D gel electrophoresis

Minimize reagent cost andwaste

Optimal combination of plseparation and fast run times

Fastest turnaround of 2-D datafor 2-D-in-a-day capability

Capability for <1 hr 2-D runsfor 2-D-in-a-day results

Can accommodate up to 4 gelsand is available in xi or XL format
for running a variety of gel sizes

Run up to 12 mini handcast
or precast gels with theMini-PROTEAN Dodeca cellRun up to 12 midi handcast
or precast gels with theCriterion Dodeca cell

Advantages

Wing clamp assembly allows
easy, fast setup and leak-free
operation

Run up to 12 gels with the
PROTEAN Plus Dodeca cellCompatible Gel Formats				
Precast		

Mini-PROTEAN precast gels(page 196)

			

Criterion, Criterion TGX,
and Criterion XT precast gels(page 202)

Handcast		Mini-PROTEAN empty cassettes(pages 196, 199)

Criterion empty cassettes(page 208)

				Mini-PROTEAN casting
plates (page 194)

PROTEAN II casting plates(page 209)

PROTEAN Plus casting
equipment (page 212)

Electrophoresis Cells
		
		
		
		

Mini-PROTEAN Tetra
Criterion (page 201)
(page 192)
Criterion Dodeca (page 201)
Mini-PROTEAN 3 Dodeca 		(page 195)		
continues

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Electrophoresis and Blotting | 181

Protein Electrophoresis
bio-rad.com/proteinelectroblot

Vertical Electrophoresis System Selection Guide
Mini-PROTEAN System		Criterion System

PROTEAN II System

PROTEAN Plus System

Gel Dimensions				(W x L x thickness) Mini-PROTEAN precast gels:
Criterion precast gels:			
8.6 x 7.2 x 0.1 cm
13.3 x 8.7 x 0.1 cm		Gel Cassette Dimensions				(W x L)10.0 x 8.0 cm
15.0 x 10.6 cm
20.0 x 18.3 cm
18.5 x 20.5 cm						
20.0 x 20.5 cm						
25.0 x 20.5 cm
Compatible Transfer Systems			
Wet/tank transfer
Mini Trans-Blot cell (page 237)
Criterion wire blotter (page 238)		
			

Criterion blotter (page 238)

Criterion plate blotter (page 238)

				Semi-dry transfer
			Trans-Blot Plus cell 239

Trans-Blot Plus cell 239

Trans-Blot Plus cell 239

Trans-Blot SD cell (page 236)

Trans-Blot SD cell (page 236)

Trans-Blot Turbo (page 235)

Trans-Blot Turbo (page 235)

Trans-Blot SD cell (page 236)

Precast Gels
Bio-Rad offers a broad range of precast gels, including two size formats of polyacrylamide gels for a number of vertical protein andnucleic acid electrophoresis applications, and one set of agarose gels for horizontal nucleic acid electrophoresis. These gels are partof complete systems of compatible electrophoresis and blotting cells. Refer to the following table to select the appropriate gel type andbuffers for your polyacrylamide gel–based applications.
Availability of Precast Gel Types Based on Application
Gel Type

Application

Sample Buffer

Running Buffer

TGX••						SDS-PAGE
Native PAGE

Laemmli
NativeTris/glycine/SDS
Tris/glycine

Tris-HCl	­ 		•						SDS-PAGE
Native PAGE

Laemmli
NativeTris/glycine/SDS
Tris/glycine

Stain-Free•

SDS-PAGE

Laemmli

Tris/glycine/SDS•

SDS-PAGE for small to large proteinsXT

XT MOPS or XT MES

Tris-acetate••						SDS-PAGE for large proteins
Native PAGE

XT
NativeXT Tricine
Tris/glycine

Tris-Tricine

Mini-PROTEAN		

•Bis-Tris•

Criterion•

SDS-PAGE for peptides, small proteins

TricineTris/Tricine/SDS

IEF			

•

IEF

IEF

Anode and cathode buffer

TBE

•

•

dsDNA separation

Nucleic acid

Tris/boric acid/EDTA

TBE-urea		•		•

ssDNA and RNA separation

TBE-urea

Tris/boric acid/EDTA

In general, single-percentage gels will best separate bands that are close in MW. If your sample contains a broad range of MWs,a gradient gel allows both high and low MW bands to be resolved on the same gel. Molecules with a range of sizes can be separated
on linear gradient gels because the larger pore sizes allow resolution of larger molecules, while pore sizes that decrease toward thebottom of the gel restrict excessive separation of small molecules.

182 | Electrophoresis and Blotting

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Protein Electrophoresis
Power Supplies

bio-rad.com/powersupplies

Power Supplies
Bio-Rad offers a complete line of power supplies that are certified to IEC 1010-1, EN 61010 — the most rigorous
international safety standard — to ensure the highest personal and environmental protection.
Power Supply Selection Guide
Technique and									Recommended
Gel or Tube Size
Typical Conditions** (Initial)
Typical Conditions** (Final)
Apparatus(W x L x Thickness),* Qty
W
V
mA
W
V
mA

TypicalRun
Time

PowerPac
Power
SupplyLaemmli (SDS), O’Farrell Second Dimension (SDS)PROTEAN II xi cell
160.0 x 160.0 x 1.5 mm, 2 gels
PROTEAN II XL cell183.0 x 200.0 x 1.5 mm, 2 gels
Criterion cell
133.0 x 87.0 x 1.0 mm, 2 gels
Criterion cell
133.0 x 87.0 x 1.0 mm, 2 gels
Mini-PROTEAN Tetra cell
83.0 x 73.0 x 1.0 mm, 4 gels
Mini-PROTEAN Tetra cell
83.0 x 73.0 x 1.0 mm, 4 gels
Mini-PROTEAN Tetra cell
83.0 x 73.0 x 1.0 mm, 4 gels

5 hr
5 hr
55 min20–26 min35–45 min15 min10 min

HV or Universal
HV or Universal
Basic or HC
Basic, HV, or Universal
Basic or HC
Basic, HV, or Universal
HV or Universal

—
—
—
—
—
—
—

High-Throughput Electrophoresis
Criterion Dodeca cell
133.0 x 87.0 x 1.0 mm, 12 gels —100
100200(C)
300(C)
200(C)
300(C)
400(C)

35(C)
35(C)
200
224
240
360
456

—
—
—
—
—
—
—

350
350
200(C)
300(C)
200(C)
300(C)
400(C)

35(C)
35(C)
80
164
120
228
416

200(C)

1,000–1,400—

200(C)

400–500 55 min

HC or Universal—

200(C)

600—

200(C)

360

45 minHC or Universal

IEF, O’Farrell First Dimension
PROTEAN II xi cell
150.0 x 1.5 mm tubes,—		
4 (minimum)		

800(C)

3.5—

800(C)<1

16 hr

HV or Universal
HV or Universal

Mini-PROTEAN 3
Dodeca cell

83.0 x 73.0 x 1.0 mm, 12 gels

Mini-PROTEAN II
tube cell75.0 x 1.0 mm tubes,—
8 (minimum)		

750(C)

1—

750(C)<1

3–4 hrPreparative PAGE
Model 491 prep cell
Mini prep cell

—
—

300
200

40
5

10(C)
1(C)

400
300

30
3

3–8 hr
HV or Universal
3–8 hr	HV or Universal

10(C)
1(C)

Protein Electroelution
Model 422
6 samples
—
200
60(C)
—
150
60(C)
3–4 hrelectro-eluter									Basic, HV,or Universal

Polyacrylamide Analytical Electrofocusing
Model 111 mini IEF cell
125.0 x 65.0 x 0.4 mm
			
			

—
—
—

100(C)
200(C)
450(C)

6
6
4

—
—
—

100(C)
200(C)
450(C)

4
4
1

15 min15 min1 hr

HV or Universal

DNA Restriction Analysis (Horizontal Mode)
Sub-Cell GT cell
150.0 x 200.0 x 5.0 mm
Mini-Sub Cell GT cell
70.0 x 100.0 x 5.0 mm
Wide Mini-Sub Cell GT cell 150.0 x 100.0 x 5.0 mm

—
—
—

80(C)
50(C)
50(C)

55
25
35

—
—
—

80(C)
50(C)
50(C)

60
30
40

4 hr
2 hr
2 hr

Basic or HC
Basic
Basic

83.0 x 73.0 x 0.75 mm, 2 gels—

100(C)

250—

100(C)

450

1 hr

HC

133.0 x 87.0 x 1.0 mm, 2 gels
133.0 x 87.0 x 1.0 mm, 2 gels——

100(C)
100(C)

250
650

—
—

100(C)
100(C)

450
1,600

1 hr
30 minHC
HC

200.0 x 160.0 x 1.5 mm, 1 gel —200.0 x 160.0 x 1.5 mm, 1 gel —200.0 x 160.0 x 1.5 mm, 1 gel —265.0 x 280.0 x 1.5 mm, 3 gels —60(C)100–150(C)50–100(C)
100(C)

210–250
1,000–1,600
1,600
3,000

—
—
—
—

60(C)
100–150(C)50–100(C)
100(C)

210–250
1,000–1,600
1,600
3,000

5 hr
1–5 hr30 min30 min–1 hr

HC
HC
HC
HC

250.0 x 180.0 x 1.5 mm
150.0 x 150.0 x 6.0 mm

15(C)
15

500
650(C)

—
—

15(C)
25

200
650(C)

15–30 min10–30 minHC
HC

Western Blotting
Mini Trans-Blot cell
Criterion blotter
Wire electrodes
Plate electrodes
Trans-Blot cell
Wire electrodes
Plate electrodes
High-intensity transfer
Trans-Blot Plus cell
Semi-Dry Blotting
Trans-Blot SD cell
Protein
DNA/RNA——* Sizes shown are typical for the corresponding apparatus. For running conditions for additional sizes, see the product instruction manuals.
** (C) = constant; typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.

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Electrophoresis and Blotting | 183

Protein Electrophoresis
Power Supplies

bio-rad.com/powersupplies

PowerPac Power Supply Specifications
Output range (programmable)
Volts
Current
Power

PowerPac BasicPowerPac HC

PowerPac HVPowerPac Universal

10–300 V
4–400 mA
75 W (maximum)

5–250 V
0.01–3.0 A
1–300 W

20–5,000 V
0.01–500 mA
1–400 W

10–500 V
0.01–2.5 A
1–500 W

Constant voltage,
constant current, or
constant power

Constant voltage, constantcurrent, constant power,
or constant temperature

Constant voltage,
constant current, or		constant power

Type of output
Constant voltage or(with automatic crossover)constant current
			
Timer

1–999 min

1 min–99 hr, 59 min

1 min–99 hr, 59 min

1 min–99 hr, 59 min

Volt-hour control——• (99,000 V-hr)• (99,000 V-hr)

Pause/resume function

•

•

•

•

Display
3-digit LED
16-character x 2-line LCD
				

128 x 64 pixel, backlit
graphics LCD

128 x 64 pixel, backlit		graphics LCD

Programmable methods—			Stores 9 methods,
each with up to 9 steps

Stores 9 methods,
each with up to 9 steps

1 method up to 3 steps,
no storage capability

Real-time clock

—

—

•

•

Automatic recovery after
power failure

•

•

•

•

Via temperature probe;
30–90ºC ± 2ºC—

Temperature control——
				Microampere readout—

Safety features
No-load detection;		sudden load change		detection; overload/		
short-circuit detection;		overvoltage protection						Operating conditions		

0–40ºC;
0–95% humidity

—

•

—

No-load detection;
sudden load change
detection; groundleak detection;
overload/short-circuit
detection; overvoltage
protection

No-load detection;
sudden load change
detection; ground leakdetection; arc detection;
overload/short-circuit
detection; overvoltage
protection

No-load detection;
sudden load change
detection; groundleak detection;
overload/short-circuit
detection; overvoltage 		
protection

0–40ºC;
0–95% humidity

0–40ºC;
0–95% humidity

0–40ºC;
0–95% humidity

Stackable

•

•

•

•

Number of output jacks

4 sets in parallel

4 sets in parallel

4 sets in parallel

4 sets in parallel

Regulatory

EN-61010, CE

EN-61010, CE

EN-61010, CE

EN-61010, CE

Input power (actual)		

90–120 or 198–264 VAC,
50/60 Hz, autoswitching

90–120 or 198–264 VAC,
50/60 Hz, autoswitching

90–120 or 198–264 VAC,
50/60 Hz, autoswitching

90–120 or 198–264 VAC,
50/60 Hz, autoswitching

Dimensions (W x D x H)

21.0 x 24.5 x 6.5 cm

25.0 x 28.5 x 8.0 cm

27.5 x 34.0 x 10.0 cm

27.5 x 34.0 x 10.0 cm

Weight

1.1 kg (2.4 lb)

2.0 kg (4.4 lb)

2.85 kg (6.3 lb)

2.5 kg (5.5 lb)

184 | Electrophoresis and Blotting

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Protein Electrophoresis
Power Supplies

bio-rad.com/powersupplies

See Also

PowerPac Basic Power Supply■■■

Mini-PROTEAN
Tetra cell:
page 192.

Recommended for basic applications
Compact, stackable
Constant voltage or constant current output

Criterion cell:
page 201.
Sub-Cell systems:
page 255.

For More Information
Request or download bulletin: 6371

Ordering Information
Catalog #Description

1645050

PowerPac Basic Power Supply, 100–120/220–240 V

Price, U.S. $360.00See Also

PowerPac HC High-Current Power Supply
■

■

■

■

Criterion blotter:
page 238.

Recommended for high-current applications
Output of 250 V, 3.0 A, 300 W
2-line, 16-character LCD for programming
Constant voltage, constant power, or constant current output

Mini Trans-Blot cell:
page 237.
Dodeca cells:
pages 195, 201.

For More Information
Request or download bulletin: 6371

Ordering Information
Catalog #Description

1645052

PowerPac HC Power Supply, 100–120/220–240 V

Price, U.S. $820.00See Also

PowerPac HV High-Voltage Power Supply■■

Model 491 prep celland mini prep cell:
page 229.

Ideal for IEF and DNA sequencing
Output of 5,000 V, 500 mA, and 400 W

Optional temperature probe monitors gel temperature between 30–90°C during electrophoresis.
The probe attaches to the glass plate and sends temperature data to the power supply, which
adjusts the power output to maintain a constant temperature during electrophoresis.
For More Information
Request or download bulletin: 6371

Ordering Information
Catalog #Description

1645056
16450591655058

PowerPac HV Power Supply, 100–120/220–240 V
PowerPac HV Power Supply with Temperature Probe, 100–120/220–240 V
PowerPac Temperature Probe

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $2,325.00
2,725.00
280.00Electrophoresis and Blotting | 185

Protein Electrophoresis
Power Supplies

See Also

bio-rad.com/powersupplies

PowerPac Universal Power Supply

High-throughput
electrophoresis
systems:
pages 195, 201.

■■

Western blotting:page 232.

For all applications from mini vertical and high-throughput electrophoresis to blotting
Protocol binder and test box to support IQ/OQ within GLP- and FDA-regulated environments

Wireless run data transfer software organizes, displays, prints, analyzes, exports, and annotates run
data from the power supply. Data can be sent directly to a PC with a peripheral IR receiving device.
For More Information
Request or download bulletin: 6371

Ordering Information
Catalog #Description

1645070

PowerPac Universal Power Supply, 100–120/220–240 V

Price, U.S. $2,210.00

PowerPac Adaptor■■■

 onvert non–IEC-certified electrophoresis cells to fit output terminals of PowerPac
C
power supplies
Available in two sizes that fit most 2 and 4 mm banana plugs
Compatible with the discontinued PowerPac 200, 300, 1000, and 3000 power supplies

Ordering Information
Catalog #Description

16450621645064

PowerPac Adaptor, 2 mm
PowerPac Adaptor, 4 mm

186 | Electrophoresis and Blotting

Price, U.S. $
59.00
59.00

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Protein Standards

bio-rad.com/proteinstandards

Protein Standards
Standards are an integral part of every electrophoresis experiment because they help identify and characterize the molecules separated
in a gel. Prestained and unstained MW standards are available for SDS-PAGE, IEF, 2-D PAGE, and western blotting. For migration chartswith different types of gels, see pages 197 (Mini-PROTEAN TGX gels) and 203–207 (Criterion gels).
For More Information
Download bulletins: 2414 and 3118
Request or download bulletin: 2998
Protein Standards Selection Guide

Dual Xtra

Dual Color

All Blue

Unstained

IEF

2-D

Molecular weight(MW)/pI range, kD

10–250 kD

10–250 kD

2–250 kD

10–250 kD

10–250 kD

10–250 kD

4.45–9.6 pI

17.5–76 kD
4.5–8.5 pI

10–250 kD

Number of proteins

10

10

12

10

10

10

9

7

10

Standard
Plugs***

Kaleidoscope

Specialty Standards

WesternC

Precision Plus Protein Standards

Electrophoresis
SDS-PAGE

•

•

•

•

•

•

–

–

•

Accurate MW
estimation

•

•

•

•

•

•

–

–

•

Multicolored

•

•

•

•

–

–

–

–

–

Coomassie staining

•

•

•

•

•

•

•

•

•

Fluorescent staining

–

–

–

–

–

•

–

•

•

2-D electrophoresis

–

–

–

–

–

–

–

•

–

IEF

–

–

–

–

–

–

•

–

–

Plug format for use in gelswith no reference well

–

–

–

–

–

–

–

–

•

•

•

•

•

•

–

–

–

–

Blotting
Monitoring transfer efficiency
Coomassie staining

•

•

•

•

•

•

–

•

•

Fluorescent staining

–

–

–

–

–

•

–

•

•

Fluorescent blotting*

•

•

•

•

•

–

–

–

–

Immunodetection**

•

–

–

–

–

•

–

–

•

Catalog Numbers
Single unit

1610385

1610375

1610377

1610374

1610373

1610363

161031016103201610378

Value pack of 5 units

1610398

1610395

1610397

1610394

1610393

1610396–––*	These standards have fluorescent properties and can be used for fluorescent blotting applications. See bulletin 5723 for details on using Precision Plus Protein WesternC
standards for fluorescent multiplexing. Precision Plus Protein Dual Xtra standards (#1610377) are recommended for fluorescent blot analysis of proteins between 5–250 kD.
**	Immunodetection via addition of a Precision Protein StrepTactin and horseradish peroxidase (HRP) or StrepTactin and alkaline phosphatase (AP) conjugate, which will bind to
the internal Strep-tags on the proteins.
*** 24 unstained plugs for 2-D gels.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 187

Protein Electrophoresis
Protein Standards

bio-rad.com/proteinstandards

Recombinant Prestained and Unstained Standards
Precision Plus Protein standards offer accurate and consistent recombinant protein standards for electrophoresis andwestern blotting experiments. These protein standards offer a good balance between band sharpness and brightness,accurate MW estimation, reproducible migration patterns, and excellent blotting results.

See Also
Vertical
electrophoresis
systems:
page 192.
Horizontal
electrophoresis
systems:
page 254.
Electrophoresisand blotting buffers:
pages 214, 245.
Proteinelectrophoresis
stains:page 221.
Protein blotting
stains: page 253.
ChemiDoc MP
imaging system:
page 285.

Precision Plus Protein Standards
Precision Plus Protein standards contain up to 12
recombinant bands that exhibit reproducible and consistentmigration regardless of staining. This family of standards
includes six unique options – one unstained, four prestained(All Blue, Dual Color, Dual Xtra, Kaleidoscope), and one
multi-application standard, WesternC. Features include:
■

■

■

■

■

MW, kD
—250
—150
—100

 eproducible and accurate migration pattern,Rallowing MW estimation— 75

 xceptional band sharpness, providing clear confirmation
Eof electrophoretic separation— 50

 trep-tag affinity sequence, allowing detection andS
MW determination on western blots— 37

 atural fluorescence properties, allowing fluorescentNand multiplex fluorescent detection

— 20

— 25

— 15
— 10

 uperior compatibility with TGX Stain-Free precast gelsS
(Unstained and All Blue)

Improved Dual Color Standard
Brighter for easier identification of target proteins

Dual Color Unstained

All Blue

Kaleidoscope WesternC

—

5

—

2

Dual Xtra■■

Stronger band intensity throughout blot development■

Sharper for more accurate molecular weight estimation

Precision Plus Protein WesternC Standards
Precision Plus Protein WesternC is a versatile, multiapplication protein standard offering colorimetric,chemiluminescent, and fluorescent properties all in one.
WesternC contains ten prestained bands from 10–250 kD
that provide electrophoretic confirmation on a gel,verification of transfer efficiency on a blot, and detection
and MW estimation on fluorescent western blots.
Features include:
■

■

■

■

■

■

The Precision Plus Protein standards family.
MW, kD
250—
150—
100—
75—
50—
37—

 eproducible and accurate migration pattern,Rallowing MW estimation20—

 xceptional band sharpness, providing clear confirmation
Eof electrophoretic separation10—

 hemiluminescent detection and MW determination onCwestern blots when probed with StrepTactin conjugates
 luorescent and multiplex fluorescent detection whenF
excited with red (~635 nm) or green (~532 nm) channels
 restained blue standards, with three pink high-intensityPreference bands at 25, 50, and 75 kD

25—

15—

Prestained Chemiluminescent Fluorescent

Precision Plus Protein WesternC standard and its multiple
applications offer many detection options.
For More Information
Download bulletins: 5561, 2847 and 5576; multiplex
fluorescence detection — 5685 and 5723

Ready-to-use formulation

188 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Protein Standards

bio-rad.com/proteinstandards

1

2

3

4

5

6

7 8

9

Individual
Precision Plus Protein
standard withintegrated Strep-tag
sequence

Add
StrepTactin-AP
or -HRP
conjugate
Western blot detection of 27 kD protein and Precision Plus
Protein WesternC standards using the Immun-Star WesternC
chemiluminescence detection kit. Maximum sensitivity achievablewith the Immun-Star WesternC kit is in the mid-femtogram range. A gelrun with 5 µl of Precision Plus Protein WesternC standards (lane 1) anda dilution series of E. coli lysate containing an overexpressed 27 kD
protein (lanes 2–9) was transferred to a nitrocellulose membrane. The
dilutions were: 200, 150, 100, 75, 50, 25, 12, and 6 ng. The blot wasprobed with a primary antibody specific for the 27 kD protein, thenincubated with StrepTactin-horseradish peroxidase (HRP) anda secondary antibody conjugated to HRP. After incubation in the
Immun-Star WesternC detection solution for 5 min, the blot was
imaged using the ChemiDoc XRS system.

StrepTactin
conjugate binds
Strep-tag sequenceAddsubstrate
Colorless substrate
comes in contact
with StrepTactin-AP
or -HRP conjugate

For More Information
Web: bio-rad.com/pppstandards

Precision Plus Protein Standard Plugs for 2-D Gels
Precision Plus Protein standard plugs allow easy, quick, andclean loading of MW standards on any gel. The plugs areespecially useful for vertical 2-D gels with no reference well.
Precision Plus Protein unstained standards are cast in 1 mm
thick agarose plugs for easy storage, handling, and loading.
Load concentrations have been optimized for Silver Stain
Plus and Bio-Safe Coomassie stains.

Substrate
convertedto coloredcompoundStandard band isvisualized by AP or
HRP enzymaticconversion ofsubstrate to coloredcompound or
chemiluminescence

Precision Plus Protein standard plugs come in easy-to-use
snap-off molds in packs of 24 (one application per plug).
Advantages include:■■■Unchanging MWs, so band sizes are easy to remember
A ready-to-use, load-and-go format — just snap, twist,
and load the plug onto a gel

Overview of the StrepTactin detection system.

Strep-tag amino acid sequence for detection and MW
estimation on western blots

Precision Plus Protein Standard Plugs

For More Information
Web: bio-rad.com/standardplugs
Download bulletin: 3036

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 189

Protein Electrophoresis
Protein Standards

bio-rad.com/proteinstandards

Precision Plus Protein Standards Specifications
Product

VolumeNumber of Applications

Unstained

1 ml

100

1 year at –20°C

All Blue

500 µl50

6 months at 4°C or 1 year at –20°C

Dual Color

500 µl50

6 months at 4°C or 1 year at –20°C

Kaleidoscope

500 µl50		

1 year at –20°C

Dual Xtra

500 µl50		

6 months at 4°C or 1 year at –20°C

WesternC

250 µl50		

1 year at –20°C

250 µl standard50		
125 µl HRP conjugate		

1 year at –20°C

WesternC pack*		
Unstained plugs

24 plugs

24		

Shelf Life

1 month at 4°C (once opened)* WesternC pack comes with WesternC protein standard and StrepTactin-HRP conjugate, needed for colorimetric or chemiluminescent blot detection.
StrepTactin-AP conjugate is also available (#1610382).

Ordering Information
Catalog #Description

1610363
16103961610373
1610393
1610374
1610394
1610375
1610395
1610385*
1610398*

Precision Plus Protein Unstained Standards, 1,000 µl, 100 applications
Precision Plus Protein Unstained Standards Value Pack, 5 x 1,000 µl, 500 applications
Precision Plus Protein All Blue Standards, 500 µl, 50 applications
Precision Plus Protein All Blue Standards Value Pack, 5 x 500 µl, 250 applications
Precision Plus Protein Dual Color Standards, 500 µl, 50 applications
Precision Plus Protein Dual Color Standards Value Pack, 5 x 500 µl, 250 applications
Precision Plus Protein Kaleidoscope Standards, 500 µl, 50 applications
Precision Plus Protein Kaleidoscope Standards Value Pack, 5 x 500 µl, 250 applications
Precision Plus Protein WesternC Pack, 250 µl WesternC standard, 125 µl HRP conjugate, 50 applications
Precision Plus Protein WesternC Pack, Value Pack, 5 x 250 µl WesternC standard,
5 x 125 µl HRP conjugate, 250 applications
Precision Plus Protein WesternC Standards, 250 µl, 50 applications
Precision Plus Protein WesternC Standards Value Pack, 5 x 250 µl, 250 applications
Precision Plus Protein Dual Xtra Standards, 500 µl, 50 applications
Precision Plus Protein Dual Xtra Standards Value Pack, 5 x 500 µl, 250 applications

1610376*
1610399*
1610377
1610397

Price, U.S. $91.00
395.0097.00
425.00125.00515.00135.00550.00200.00840.00155.00640.00135.00555.00StrepTactin Conjugates for Precision Plus Protein Standards
1610380
Precision Protein StrepTactin-HRP Conjugate, 300 µl, 150 applications
1610381
Precision Protein StrepTactin-HRP Conjugate, 125 µl, 50 applications
1610382
Precision Protein StrepTactin-AP Conjugate, 300 µl, 150 applications

90.00
31.00
90.00

Precision Plus Protein Standard Plugs
1610378
Precision Plus Protein Standard Plugs, unstained, 24 applications

120.00Clarity Western ECL Substrate
1705060
Clarity Western ECL Substrate, 200 ml
1705061
Clarity Western ECL Substrate, 500 ml

135.00
255.00* Note that StrepTactin (-HRP or -AP) conjugate is needed for colorimetric or chemiluminescence blots.

190 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Protein Standards

bio-rad.com/proteinstandards

Specialty Standards (IEF and 2-D SDS-PAGE)IEF Standards
IEF standards allow dependable and reproducible pI calibrationin native polyacrylamide or agarose IEF gels. IEF standards area mixture of nine native proteins with pIs ranging from 4.45–9.6.
To monitor focusing, five of the nine proteins are naturallycolored. The standards are provided in a stable aqueous
solution and require no reconstitution or dilution prior to use.

Basic end
1
2

3
4
5

Constituent Proteins of IEF Standards*
Protein

Color

pI

1. Cytochrome c
2. Lentil lectin (3 bands)
3. Human hemoglobin C
4. Human hemoglobin A
5. Equine myoglobin (2 bands)
6. Human carbonic anhydrase
7. Bovine carbonic anhydrase
8. b-lactoglobulin B
9. Phycocyanin (3 bands)

Red
—
Red
Red
Brown
—
—
—
Blue

9.6
7.8, 8.0, 8.2
7.5
7.1
6.8, 7.0
6.5
6.0
5.1
4.45, 4.65, 4.75

* Because the IEF standards are in native form, they cannot be usedwith reducing or denaturing agents such as urea, b-mercaptoethanol,
or dithiothreitol. For calibration of IEF tube gels containing urea, use
2-D SDS-PAGE standards.

6

7

8

9
Acidic end

IEF standards stained with IEF gel stain. Run on Criterion
Tris-HCl (left) and Ready Gel IEF gels (right).

Ordering Information
Catalog #Description

1610310IEF Standards, 250 µl

Price, U.S. $120.002-D SDS-PAGE Standards

ProteinpI

MW, kD

1. Hen egg white conalbumin
2. Bovine serum albumin (BSA)
3. Bovine muscle actin
4. Rabbit muscle GAPDH
5. Bovine carbonic anhydrase
6. Soybean trypsin inhibitor
7. Equine myoglobin

6.0, 6.3, 6.6
5.4, 5.5, 5.6
5.0, 5.1
8.3, 8.5
5.9, 6.0
4.5
7.0

76
66
43
36
31
21.5
17.5

43,000

8.5

1

7.0

5.9

2

6.6

5.5

4.5
76,00066,200MW

Constituent Proteins of 2-D SDS-PAGE Standards

5.1

pl

2-D SDS-PAGE protein standards provide calibrated
references for the pI and MW of proteins in 2-D SDS-PAGE
applications. These standards consist of seven reduced,
denatured proteins that can be visualized with silver or
Coomassie Blue stains and require no dilution prior to use.

3

36,00031,0004
521,50017,50067Migration pattern of 2-D SDS-PAGE standards. First dimension
separation was performed with 7 cm ReadyStrip IPG strips. Second
dimension separation was achieved with the Mini-PROTEAN II cell.

Ordering Information
Catalog #Description

1610320

2-D SDS-PAGE Standards, 500 µl

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $195.00Electrophoresis and Blotting | 191

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro

Mini-Format 1-D Electrophoresis Systems
The Mini-PROTEAN system includes the four-gel Mini-PROTEAN Tetra cell and the high-throughput, 12-gel
Mini-PROTEAN 3 Dodeca cell. The systems are compatible with mini handcast or precast gels.

See Also
PowerPac Basic andPowerPac HC power
supplies: page 185.
Premixed buffers:
page 214.
Mini-PROTEAN
precast gels:page 196.

Mini-PROTEAN Tetra Cell
The Mini-PROTEAN Tetra cell is ideal for vertical mini gelelectrophoresis. This electrophoresis cell accommodates
1–4 precast or handcast gels. Easy to assemble,
the Mini-PROTEAN Tetra cell has a patented sealingmechanism* that prevents assembly errors. The
Mini-PROTEAN Tetra cell offers the following advantages:

Mini-PROTEAN Tetra cell components:
5. Spacer plates.
1. Lid and tank.
2. Combs.
6. Short plates.
7. Sample loading guides.
3.	Mini-PROTEAN TGX	precast gels.
8. Casting frame.
9. Casting stand.
4. Gel releasers.

1

Loading and Running
Patented sample loading guides** prevent skipped orrepeated loading lanes

9

■

■

 ell runs up to four gels (10.0 x 8.3 cm) using two
Crunning modules
8

Modular Cells for Many Applications
Interchangeable modules convert a Mini-PROTEAN Tetracell into a Mini Trans-Blot electrophoresis transfer cell forwestern blotting■

Gel CastingGround glass plates with permanently bonded spacers
and improved casting gaskets assure perfect alignment
and leakproof casting

7

6

2
3

■

■

■

■

■

■

Casting frames*** with simple cam closure provide
precision alignment on any flat surface
Side-by-side casting stand*** allows access to both gelssimultaneously, and the spring-loaded lever creates atight seal against the silicon rubber gasket
Plastic combs*** do not inhibit polymerization and havebuilt-in ridges to eliminate air contact during gel castingfor uniform gel polymerization

4

5

Mini Trans-Blot electrophoresis transfer cell components:
1. Lid.
2. Tank.
3. Electrode assembly.
4.	Companion running
module.
5.	Mini Trans-Blotmodule.
15

 lass plates and combs are labeled with thickness
Gand number of wells for instant identification
Thick glass spacer plates reduce breakage

Configuring Your Own Electrophoresis Cell
You can choose one of the preset configurations, suchas #1658000 (10-well, 0.75 mm) or #1658001 (10-well,
1.0 mm). To configure your own electrophoresis cell,order the Mini-PROTEAN Tetra cell (#1658004) andselect a casting module from the ordering information(see page 194).

2

3

4

For More Information
Web: bio-rad.com/tetra
Request or download bulletin: 5535

* U.S. patents 6,436,262, ** 5,656,145, and *** 6,162,342.

192 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro

Maximum Sample Volume per Well for Mini-PROTEAN Tetra Combs
					
Number or Type of Wells

Well Width, mm

0.75 mm

5		
12.70
70 µl
9		
5.08
33 µl
10
5.08
33 µl
15
3.35
20 µl
IPG
6.20
—
Prep/2-D			
Reference well
3.10
13 µl
Sample well
67.44
310 µlComb Thickness
1.0 mm

1.5 mm105 µl
44 µl
44 µl
26 µl
420 µl160 µl
66 µl
66 µl
40 µl
730 µl17 µl
400 µl30 µl680 µlSpecifications
1–4
Number of gels
Mini-PROTEAN
Precast gels
Cast using Mini-PROTEAN spacer plates
Handcast gels
Cassette size (W x L) Precast: 10.0 x 8.3 cm
Glass plate size (W x L) Short plate: 10.1 x 7.3 cm
Spacer plate: 10.1 x 8.2 cm
		Total buffer volume for 2 gels
700 mlTotal buffer volume for 4 gels
1,000 ml
Typical run times for SDS-PAGE 35–45 min (at 200 V constant)
Recommended power supply	See Power Supply Selection Guide, p. 183
Dimensions (W x L x H)
12.0 x 16.0 x 18.0 cm
Weight1 kg (2.2 lb)

Ordering Information
Catalog #Description

1658001FC

 ini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 4-gel system includes 5 combs, 1 set of glass
M
plates (5 short plates and 5 spacer plates), 2 casting stands, 4 casting frames, sample loading guide,
electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 2-gel system includes 5 combs, 1 set of glass
plates (5 short plates and 5 spacer plates), casting stand, 2 casting frames, sample loading guide,
electrode assembly, tank, lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 2-gel system includes 5 combs, 1 set of glass
plates (5 short plates and 5 spacer plates), casting stand, 2 casting frames, sample loading guide,
electrode assembly, tank, lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Cell for Mini Precast Gels, 4-gel system includes electrode assembly, companion
running module, tank, lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Cell for Mini Precast Gels, 2-gel system includes electrode assembly, tank,lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Cell, 10-well, 1.5 mm thickness; 4-gel system includes 5 combs, 1 set of glass
plates (5 short plates and 5 spacer plates), 2 casting stands, 4 casting frames, sample loading guide,
electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Cell, 10-well, 1.5 mm thickness; 2-gel system includes 5 combs, 1 set of glass
plates (5 short plates and 5 spacer plates), casting stand, 2 casting frames, sample loading guide,
electrode assembly, tank, lid with power cables, mini cell buffer dam

1658002FC*
1658003FC*
1658004
1658005*
1658006FC

1658007FC*

Price, U.S. $935.00740.00740.00615.00470.00935.00740.00Mini-PROTEAN Tetra Systems
1658025FC
Mini-PROTEAN Tetra Cell and PowerPac Basic Power Supply, includes #1658001 and #1645050
1658026FC
Mini-PROTEAN Tetra Cell and PowerPac Universal Power Supply, includes #1658001 and #1645070
1658027FC
Mini-PROTEAN Tetra Cell and PowerPac HC Power Supply, includes #1658001 and #1645052
1658028FC
Mini-PROTEAN Tetra Cell and PowerPac HV Power Supply, includes #1658001 and #1645056
1658029FC
Mini-PROTEAN Tetra Cell and Mini Trans-Blot Module, includes #1658001 and #1703935
1658030
Mini-PROTEAN Tetra Cell for Mini Precast Gels and Mini Trans-Blot Module, includes #1658004 and #1703935
1658033FC
Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply, includes #1658001,#1703935, and #1645050
1658034FC
Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac Basic Power
Supply, includes #1658004, #1703935, and #1645050
1658035FC
Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac HC Power Supply, includes #1658001,#1703935, and #1645052
1658036
Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac HC Power Supply,
includes #1658004, #1703935, and #1645052

1,225.00
3,090.00
1,685.00
3,040.00
1,280.00
985.001,515.00
Inquire2,060.00
1,850.00
continues

The catalog numbers above with an “FC” suffix come with a 10% TGX Stain-Free FastCast kit (1610182). To place an order without a FastCast kit,
remove the “FC” suffix from the catalog number.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 193

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro

Ordering Information
Description

0.75 mm

1.0 mm

1.5 mmPrice, U.S. $Casting Modules**5-Well9-Well
10-Well
15-Well
Prep/2-D Well
IPG Well

1658008
1658009
165801016580111658012—1658013—16580151658016—165801816580191658020165802116580221658023—405.00405.00405.00405.00405.00405.001653357
1653358
16533591653360
1653361165336216533631653364
16533651653366
16533671653368

38.00
38.00
38.00
38.00
38.00
38.00

Mini-PROTEAN Combs (5) for Hand Casting with Glass Plates
5-Well1653352
9-Well1653353
10-Well
1653354
15-Well
1653355
Prep/2-D + 1 Reference Well
1653356
IPG Well—Catalog #Description

Price, U.S. $Handcast Gel Accessories and Replacement Parts
1658051
Mini-PROTEAN Tetra Cell Casting Stand, 2 core, includes clamps for use with theMini-PROTEAN Tetra cell casting modules
Mini-PROTEAN Tetra Cell Casting Stand, 1 core, includes clamps for use with the1658052
Mini-PROTEAN Tetra cell casting modules
1653303
Mini-PROTEAN Casting Stand with Gaskets
1653304
Mini-PROTEAN Casting Frame
1653305
Mini-PROTEAN Casting Stand Gaskets, replacement, 21653308
Short Plates, 51653149
Replacement Gaskets, for electrophoresis clamping frame, green, 21653310
Spacer Plates with 0.75 mm Integrated Spacers, 51653311
Spacer Plates with 1.0 mm Integrated Spacers, 51653312
Spacer Plates with 1.5 mm Integrated Spacers, 5

320.00185.00160.00
41.00
40.00
25.00
48.00
66.00
55.00
55.00

Other Replacement Parts and Accessories
1658037
Mini-PROTEAN Tetra Electrode Assembly
1658038
Mini-PROTEAN Tetra Companion Running Module
1658039
Buffer Tank, replacement
1658040
Buffer Tank and Lid, replacement
1658041
Cell Lid with Power Cables
1653201
Sample Loading Guide, 9-well (red)1653146
Sample Loading Guide, 10-well (yellow)
1653203
Sample Loading Guide, 12-well (green)
1653132
Sample Loading Guide, 15-well (blue)
Mini Cell Buffer Dams, 2 (compatible with Mini-PROTEAN Tetra cell, Mini-PROTEAN 3 Dodeca cell,1653130
and the discontinued Mini-PROTEAN 3 cell)1653320
Gel Releasers, 5

190.00180.0061.00
185.00125.00
27.00
27.00
27.00
27.00
16.00
30.00

*		The 2-gel systems do not include the companion running module.
** Each casting module includes 2 casting stands, 4 casting frames, 5 combs, 1 set of glass plates(5 short plates and 5 spacer plates), and the appropriate sample loading guide.

194 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro

See Also

Mini-PROTEAN 3 Dodeca Cell

PowerPac HC power
supply: page 185.

The Mini-PROTEAN 3 Dodeca cell runs up to 12 minigels under identical conditions in just 35 minutes.
Features of the Mini-PROTEAN 3 Dodeca cell include:
■

■

■

■

Mini-PROTEAN
precast gels:page 196.
AnyGel stands:
page 199.

Built-in cooling coil to prevent overheating
Stirbar capability helps maintain uniform buffer tanktemperatures for run reproducibility
Easy assembly facilitated by a patented* electrophoresis
clamping frame
Convenient buffer draining via the built-in quick-connect
drain port

For More Information
Web: bio-rad.com/dodeca
Download bulletin: 2571
Specifications
Number of gels

1–12

Precast gels

Mini-PROTEAN

Handcast gels

Cast using Mini-PROTEAN 3 spacer plates and the Mini-PROTEAN 3 multi-casting chamber

Cassette size (W x L)

10.0 x 8.3 cm

Gel thickness

0.5, 0.75, 1.0, or 1.5 mm (precast gels are available only in 1.0 mm)

Total buffer volume

3.4–4.4 L

Typical running conditions

200 V constant, 600 mA, 120 W maximum

Cooling

Built-in cooling coil attaches easily to external refrigerated circulator (circulator must be purchased separately;
recommended flow rate 10–15 L/min, recommended cooling capacity ≥250 W at 20°C)

Recommended power supply

PowerPac HC

Dimensions (W x L x H)

16.2 x 41.5 x 15.0 cm

Weight5 kg (11 lb)* U.S. patent 6,436,262.

Ordering Information
Catalog #Description

1654100

 ini-PROTEAN 3 Dodeca Cell, includes electrophoresis tank with built-in cooling coil, lid with power cables,M
6 electrophoresis clamping frames, 2 buffer dams, drain line, 2 gel releasers
Mini-PROTEAN 3 Dodeca Cell and 6-Row AnyGel Stand, includes #1654100 and #1655131

1655132

Replacement Parts and Accessories
1654102
Replacement Electrophoresis Clamping Frame
1653149
Replacement Gaskets, for electrophoresis clamping frame, green, 21654104
Replacement Drain Line
1654105
Replacement Cooling Coil, includes connector tubing
1652948
Replacement Power Cables, for lid
1653320
Gel Releasers, 51653130
Mini Cell Buffer Dams, 2 (compatible with Mini-PROTEAN Tetra cell, Mini-PROTEAN 3 Dodeca cell,and the discontinued Mini-PROTEAN 3 cell)1655131
AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Mini-PROTEAN gelsIn the U.S., call toll free at 1-800-424-6723

Price, U.S. $4,165.00
4,365.00
245.0048.00
150.00150.00
73.00
30.00
16.00
295.00Electrophoresis and Blotting | 195

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

Videos
Product
Demonstration

bio-rad.com/
PROTEANprep

Product
Demonstration

bio-rad.com/
PROTEANnovex

Customer
Testimonial

Mini-PROTEAN Precast Gels
Mini-PROTEAN TGX Precast Gels
Long shelf life Mini-PROTEAN TGX precast gels accelerate
electrophoresis and blotting while delivering superior
performance. TGX gels maintain cooler temperatures athigh voltages, allowing run times as short as 15 minutes.
The gels are designed to provide Laemmli-like separation
patterns using the standard Tris/glycine/SDS running buffer
system. Mini-PROTEAN gels are compatible with theMini-PROTEAN Tetra (1–4 gels) and Dodeca (1–12 gels)
cells. These gels can also be used in the earlier
Mini-PROTEAN 3 cell model. Gel opening lever(4560000) sold separately.
Mini-PROTEAN TGX gels provide:■■bio-rad.com/
PROTEANresearchers

Product Spotlight andCustomer Testimonial

Run times as short as 15 min ransfer times as short as 3 min with theTTrans Blot Turbo transfer system■

12-month shelf life■

Laemmli format■

Inexpensive buffer system, low running costs■

 ottom-open cassette design for simple gel handlingB
and blotting

For More Information
Web: bio-rad.com/tgx
Request or download bulletins: 5535 and 5871
bio-rad.com/
PROTEANinnovate

bio-rad.com/minielectro

Mini-PROTEAN TGX Stain-Free Precast Gels
Mini-PROTEAN TGX Stain-Free precast gels combine
TGX formulation with a proprietary compound that facilitates
protein visualization in less than 5 minutes using Bio-Rad’s
Stain-Free enabled imaging systems (see page 284).
Mini-PROTEAN TGX Stain-Free precast gels eliminate
the need for staining, reducing the time to results
and improving the ease of downstream processing.
In addition to the 12-month shelf life, Laemmli buffer
system, and fast run times of the TGX formulation,
the Mini-PROTEAN TGX Stain-Free gels provide:
■

■

■

■

Empty Cassettes for Hand CastingSingle-use empty Mini-PROTEAN cassettes are available
to hand cast gels. For added convenience, cast your gelsusing AnyGel stands or the Mini-PROTEAN casting stand.
Mini-PROTEAN Gels
Mini-PROTEAN Tris-Tricine Precast GelsDesigned for separation of peptides and small proteins
with MWs <10,000■

Mini-PROTEAN TBE and TBE-Urea Precast Gels
Ideal for the separation of DNA and RNA■■■

 BE gels are suitable for electrophoresis of nucleic acids
Tfrom 50 to 2,000 bp
 BE-urea gels are best suited for the separation ofT
ssDNA and ssRNA from 60 to 200 bp

For More Information
Web: bio-rad.com/mpgels omplete protein separation, gel imaging, and dataC
analysis in 20 min
Sensitivity comparable to that of Coomassie stain
 etter reproducibility and quantitation comparedB
to staining procedures
 apability of using the same gel for chromatography,Cwestern blotting, standard staining methods, andmass spectrometry analysis

For More Information
Web: bio-rad.com/ministainfree

196 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis

Mini-PROTEAN TGX

Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro
Precision Plus Protein™ Unstained
7.5%

10%

12%

4–15%

4–20%

Any kD™

Gel Cassette Specifications
86.0 x 72.0 x 1.0 mm

Gel dimensions(W x L x thickness)Gelcassette
250
250dimensions
250
150(W x L x thickness)
150
150

Cassette material

100

100

Comb material

250

250mm
101.0 250
x 89.0 x 4.6
150
100

150

150

Styrene copolymer
Polycarbonate
100

75

100
100
75
Gel
storage conditions	Store flat at 4°C; 50
do not freeze
75

75

75
50

Mini-PROTEAN Precast Gel Selection50Guide
50

75

37

37

50
37

25

Shelf Life at Recommended Temperature*
37

TGX, TGX Stain-Free

Tris-Tricine

TBE, TBE-Urea

12 months

8–12 weeks

8–12 weeks

20

25
Recommended
Buffers			
25
37
20
20sample)
Sample(dilute 1:1 with
Laemmli
Tricine50
25

Running

20

25

37

* From date of20manufacture.
15

15

15
15

Tris/glycine/SDS

Nucleic acid, TBE-urea

Tris/Tricine/SDS

Tris/boric acid/EDTA (TBE)

10

10

10

Mini-PROTEAN Precast Gel Migration
Charts
TBE
5%
7.5%

2,000
1,500

10%

2,000
1,500
200

1,000

1,000
700

700
500

116

400

97.4

200

116

500 97.4
400
300

300

12%

2,000
1,500
1,000200
700
500
400116
97.4

300
66

100

45

45

31

50

31

50
31

300
200
97.4

2,000
1,500
1,000
700
200
500
400
300
116

8–16%

2,000
1,500
1,000
200 700500
116 400

97.5
66

66
100

300
200

200
97.4
45

100

66
100
45

50

Tris-Tricine
4–20%
Any kD

200

66

45

21.5

50

21.5

14.4

14.4
6.5

In the U.S., call toll free at 1-800-424-6723

700

700

500
400

500
400

50

300
200

140
120
100
80
60
40

300

200

21.5
21.5

21.5

14.4

14.4

6.5

6.5

14.4

500
400

2,000
1,000
140
120
100
80

700

60

400

40

100

50

2,000
1,500
1,000
700

1,500

500

60
50
40
30

16.5% 15%

500
400
300

2,000
1,500
1,000
700
500
60
400
50
40
300
30
200

200

300

20

100

100
20

20

20

300
200

100

5

20

10

5
2

50

20

500
400

15

300

10
5

50
50

Electrophoresis and Blotting | 197

2

2

1

200
100

2
50

10

100

6.5

700
25

50
10

1,000

200

100

50

1,500

25

15

15

2
50

20

25

2,000

300

100

200
50

4–
10–20%

2,000
1,500
1,000
700
500
400

2,000
1,500
1,000
700
500
400

10
200

100
31

700

Tris-Tricine

15%

2,000
1,500
1,000

TBE

TBE Urea15% 10%

5%

2,000
1,500
1,000

1,000

31

31

21.5

2,000

300
31

TBE
10% 5%

16.5%

116 1,500
97.4

45

66

100

50

2,000
1,500
1,000
200
700
500
400

4–20%

66

45

100

4–15%

116

200

200
200

TBE-Urea

TGX and SFX with10%
Broad Range Unstained Standards
15%

1

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro

Ordering Information

Description

10-Well*
30 μl

10-Well
50 μl

12-Well
20 μl

15-Well
15 μl

IPG Well†
7 cm IPG Strip

Mini-PROTEAN TGX Precast Gels
7.5% Resolving Gel
10% Resolving Gel
12% Resolving Gel
4–15% Resolving Gel
4–20% Resolving Gel
8–16% Resolving Gel
Any kD Resolving Gel

4561023**4561033**4561043**4561083**4561093**45611034569033**4561024**4561034**4561044**4561084**4561094**4561104
4569034**4561025**4561035**4561045**4561085**4561095**
4561105
4569035**4561026**4561036**4561046**4561086**4561096**4561106
4569036**456102145610314561041456108145610914561101
4569031105.00105.00105.00105.00105.00105.00105.00Mini-PROTEAN TGX Stain-Free Precast Gels
7.5% Resolving Gel
4568023
10% Resolving Gel
4568033
12% Resolving Gel
4568043
4–15% Resolving Gel
4568083
4–20% Resolving Gel
4568093
8–16% Resolving Gel
4568103
Any kD Resolving Gel
4568123456802445680344568044456808445680944568104
45681244568025456803545680454568085456809545681054568125456802645680364568046456808645680964568106
4568126456802145680314568041456808145680914568101
4568121115.00115.00115.00115.00115.00115.00115.004560003—4560005——

225.004560013—4560015

—

—

54.00

Empty Cassettes and Combs
Mini-PROTEAN
Empty Cassette***Combs(for Mini-PROTEAN
empty cassettes)

Price, U.S. $* 		Multichannel pipet compatible.
** 	Mini-PROTEAN TGX gels are available with 100 µl of dual color standard (add DC to the end of the catalog number listed).
*** Includes 50 empty cassettes. Combs sold separately in 50 pack.
†
IPG well only, no reference well. If a protein standard is needed on gel, order Precision Plus Protein Standard plugs, #1610378.
Catalog #Description

4560000

Mini-PROTEAN Precast Gel Opening Lever

Price, U.S. $5.00

Premixed Buffers for Mini-PROTEAN TGX Gels*
1610737
2x Laemmli Sample Buffer, 30 ml
1610747
4x Laemmli Sample Buffer, 10 ml
16107382x Native Sample Buffer, 30 ml
1610732
10x Tris/Glycine/SDS, 1 L
1610734
10x Tris/Glycine, 1 L

14.00
15.00
16.00
28.00
28.00

* For 5 L volume of the running buffers, see page 215.
Applications Guide
1658100
Mini-PROTEAN Gel Instruction Manual and Application Guide, online

3.00

10-Well
30 µl

10-Well
50 µl

12-Well*
20 µl

15-Well
15 µl

Price, U.S. $Mini-PROTEAN Precast Gels (2 per package)
5% TBE4565013
15% TBE-Urea
4566053*
16.5% Tris-Tricine
45630634565014*——4565015*
4566055*4563065*4565016—4563066

25.00
25.00
25.00

Description* Please allow up to 2 weeks for delivery.
continues

198 | Electrophoresis and Blotting

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Protein Electrophoresis
bio-rad.com/minielectro

Mini-Format 1-D Electrophoresis Systems

Ordering Information
Catalog #Description

Price, U.S. $Premixed Buffers for Tris-HCI Gels
1610737
2x Laemmli Sample Buffer, 30 ml
1610747
4x Laemmli Sample Buffer, 10 ml
16107382x Native Sample Buffer, 30 ml
1610732
10x Tris/Glycine/SDS, 1 L
1610734
10x Tris/Glycine, 1 L
1610772
10x Tris/Glycine/SDS, 5 L cube
1610771
10x Tris/Glycine, 5 L cube

14.00
15.00
16.00
28.00
28.00
94.00
94.00

Premixed Buffers for Tris-Tricine Gels for Peptides
1610739
2x Tricine Sample Buffer, 30 ml
1610744
10x Tris/Tricine/SDS, 1 L

14.00
86.00

Premixed Buffers for IEF Gels
1610763
IEF Sample Buffer, 30 ml
1610761
10x IEF Anode Buffer, 250 ml
1610762
10x IEF Cathode Buffer, 250 ml

14.00
11.00
57.00

See Also

Mini-PROTEAN Hand Casting Accessories

Acrylamide
gel-casting reagents:
page 217.

Empty Cassettes
Single-use empty Mini-PROTEAN cassettes are available
for hand casting a mini gel.

Premixed buffers:
page 214.

AnyGel Stands
AnyGel stands are convenient for storing glass plates of any
size gel. They are available as single row or six-row stands.
Features of the six-row stand:■■■

Perfect for high-volume 2-D proteomics studies —
accommodates up to 6 PROTEAN, 12 Criterion,or 18 Mini-PROTEAN mini gels

AnyGel Six-Row Stand

Facilitates loading IPG strips on both large-format gels
and Criterion gel sizes using a front clamp that slants thegel to an ideal angle (can also be used to load tube gels)
A stair-step design and clear clamps so gelsare clearly visible while casting and loading

The single-row AnyGel stand is ideal for processing a few
gels at a time. It accommodates one PROTEAN gel,two Criterion gels, or three Mini-PROTEAN mini gels.
AnyGel Single-Row Stand with Mini-PROTEAN Cassettes

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 199

Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems

bio-rad.com/minielectro

Ordering Information
Catalog #Description

Price, U.S. $Mini-PROTEAN Empty Cassettes and Combs
4560003
Mini-PROTEAN Empty Cassettes, 10-well, 50
4560005
Mini-PROTEAN Empty Cassettes, 12-well, 50
4560013
Mini-PROTEAN Combs, 10-well, 50
4560015
Mini-PROTEAN Combs, 12-well, 50

225.00225.0054.00
54.00

AnyGel Stands and Accessories
1654131
AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Mini-PROTEAN gels
1655131
AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Mini-PROTEAN gels
1654132
Replacement Clamps, 278.00
295.0033.00

AnyGel Stands and Electrophoresis Cells
1655133
Criterion Dodeca Cell and 6-Row AnyGel Stand, includes #1654130 and #1655131
1656020
Criterion Cell and Single-Row AnyGel Stand, includes #1656001 and #1654131

3,050.00
765.00Mini-PROTEAN 3 Multi-Casting Chamber Accessories
1654114
Acrylic Blocks, 6.0 mm, 81654115
Separation Sheets, 151653320
Gel Releasers, 51652913
Replacement Gaskets, for Mini-PROTEAN 3 multi-casting chamber, includes 3' of tubing

94.00
59.00
30.00
44.00

Combs*,** and Glass Plates for 2-D Electrophoresis
1653308
Short Plates, 51653310
Spacer Plates with 0.75 mm Integrated Spacers, 51653311
Spacer Plates with 1.0 mm Integrated Spacers, 51653312
Spacer Plates with 1.5 mm Integrated Spacers, 51653362
Mini-PROTEAN Comb, IPG well, 1.0 mm
1653368
Mini-PROTEAN Comb, IPG well, 1.5 mm
1653356
Mini-PROTEAN Comb, prep/2-D well, 0.75 mm
1653361
Mini-PROTEAN Comb, prep/2-D well, 1.0 mm
1653367
Mini-PROTEAN Comb, prep/2-D well, 1.5 mm

25.00
66.00
55.00
55.00
38.00
38.00
38.00
38.00
38.00

Model 485 Gradient Former Accessories
1652007
Gradient Pouring Needles, 21652008
Tubing Connection Kit, includes stopcock, luer taper coupling, tubing (1/8" ID, 3'), Y-connector

66.00
44.00

* Order combs separately (see combs for use with glass plates on page 194) in the Mini-PROTEAN Tetra cell section.
** For multi-well comb configurations, refer to page 194.

200 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Midi-Format 1-D Electrophoresis Systems

bio-rad.com/midielectro

Midi-Format 1-D Electrophoresis Systems
The Criterion and the Criterion Dodeca electrophoresis cells accommodate precast or handcast Criterion gels that are wider
and longer than traditional mini gels for increased throughput and separation.

See Also

Criterion Cell and Criterion Dodeca Cell

Criterion precastgels and empty
cassettes:
pages 202, 208.

Criterion Cell
The Criterion electrophoresis cell is dedicated to runningone or two midi gels (13.3 x 8.7 cm), which are wider andlonger than traditional mini gels (8.6 x 7.2 cm). With asingle Criterion gel, you can run up to 26 samples in lessthan 1 hour or accommodate 11 cm ReadyStrip IPG strips
for 2-D applications.
■■■

Criterion blotter:
page 238.
PowerPac Basicand PowerPac HC
power supplies:
page 185.
Dodeca stainers:
page 208.

Compact size that requires only 1 L of running buffer

AnyGel stands:
page 199.

 uilt-in wedge on the lid to open gel cassettes in aBsingle step ocator slots built into the tank walls to easilyLand quickly slide cassettes* into position* U.S. patent 6,093,301.

Imaging systems:
page 284.
Imaging software:
page 289.

For More Information
Web: bio-rad.com/criterioncell
Download bulletin: 2710

Criterion Dodeca Cell
The Criterion Dodeca cell has the capacity to run up to 12
handcast or Criterion precast gels simultaneously. Criteriongels accommodate 11 cm ReadyStrip IPG strips.
■

■

■

■

■

Locator slots to slide cassettes* into place withoutalignment hassles or bulky clamps
 uilt-in cooling coil to prevent overheating and ensureBthe highest resolution
Stirbar capability to maintain uniform buffer tanktemperatures for reproducible runs
A cassette opener built into the cell for easy gel accessConvenient buffer draining via the built-in quick-connect
drain port

* U.S. patent 6,093,301.

For More Information
Web: bio-rad.com/criteriondodeca
Download bulletin: 2622

Specifications

Criterion Cell			

Number of gels
Precast gels
Handcast gels
Gel size (W x L)
Gel thickness
Total buffer volume
Typical running conditions
Recommended power supply
Dimensions (W x L x H)
Weight1–2 				1–12
Criterion precast gels 			
Criterion precast gels
Gels prepared in Criterion empty cassettes
Gels prepared in Criterion empty cassettes
13.3 x 8.7 cm 			13.3 x 8.7 cm
1.0 mm				
1.0 mm
1 L 				
6L200 V constant			
200 V constant; 1 A maximum; 200 W maximum
PowerPac Basic or PowerPac HC 		PowerPac HC or PowerPac Universal
14.4 x 22.3 x 19.5 cm 			18.8 x 49.0 x 19.2 cm
0.86 kg (1.9 lb) 			
4.8 kg (11 lb)

In the U.S., call toll free at 1-800-424-6723

Criterion Dodeca Cell

Electrophoresis and Blotting | 201

Protein Electrophoresis
Midi-Format 1-D Electrophoresis Systems

bio-rad.com/midielectro

Ordering Information
Catalog #Description

Price, U.S. $Criterion Cell and Systems
1656001
Criterion Cell, includes electrophoresis buffer tank, lid with power cables, 3 sample loading guides(12+2 well, 18-well, 26-well)Criterion Cell and PowerPac Basic Power Supply, 100–120/220–240 V, includes #1656001 and #1645050
16560191656020
Criterion Cell and Single-Row AnyGel Stand, includes #1656001 and #1654131

1,100.00
765.00Replacement Parts
1656002
Criterion Replacement Electrophoresis Buffer Tank, with lower electrodes
1656003
Criterion Replacement Lid, with upper electrodes
1656004
Criterion Replacement Upper Electrode, includes prestrung platinum wire
1656005
Criterion Replacement Lower Electrode, includes prestrung platinum wire
1652948
Replacement Power Cables, for lid
1654131
AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Mini-PROTEAN gels

360.00280.00110.00185.0073.00
78.00

Criterion Dodeca Cell
1654130
Criterion Dodeca Cell, includes electrophoresis buffer tank with built-in cooling coil, lid with power cables
1654138
Criterion Dodeca Cell and PowerPac HC Power Supply, includes #1654130 and #1645052
1654139
Criterion Dodeca Cell and PowerPac Universal Power Supply, includes #1654130 and #1645070
1655133
Criterion Dodeca Cell and 6-Row AnyGel Stand, includes #1654130 and #1655131
Replacement Parts
1654135
Lower Electrode with Platinum Wire
1654136
Replacement Cooling Coil, includes connector tubing
1652948
Replacement Power Cables, for lid

See Also
Premixed buffers:
page 214.
ReadyStrip IPG strips:
page 225.
Criterion blotter:
page 238.
PowerPac
power supplies:
page 183.
Dodeca stainers:
page 208.
Criterion staining
trays: page 208.
Standards:
page 187.
Proteinelectrophoresis
stains: page 221.

585.003,050.00
3,905.00
5,360.00
3,050.00
595.0099.00
73.00

Criterion Precast Gels
Criterion precast gels include a broad selection of
midi polyacrylamide gels in single-use cassettes. This gel
size provides reproducible, high-resolution results with
fast setup, loading, and run times. The gels are wider
and longer than traditional mini gels for high-throughput
electrophoresis. Criterion gels are packaged and soldin individual units: 1–2 gels can be run in the Criterion
cell (page 201) and 1–12 gels can be run in the highthroughput Criterion Dodeca cell (page 201).
■

■

■

■

■

■

Fast run times and 12-month shelf life for Criterion TGX gels
 oom temperature storage and 12-month shelf life forR
Criterion XT Bis-Tris gels ormats that run up to 26 samples on a single gel
F
without reducing sample volume or sacrificing speed
 patented* cassette design including an integralA
upper buffer chamber that never leaks and requires
no tools to open
 ample wells that are outlined and numbered forSeasy loading

Integral buffer chamber
Gel type, expiration date,catalog, and lot numbers
Outlined andnumbered wells

Multichannel pipet–compatible combs

202 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Midi-Format 1-D Electrophoresis Systems

bio-rad.com/midielectro

Criterion TGX Precast Gels
These precast gels use the Laemmli buffer system and havea 12-month shelf life. And they maintain cooler temperaturesat elevated voltages, allowing for reduced run times.
■■

Run times as short as 20 min ransfer times as short as 7 min with theTTrans-Blot Turbo system■

12-month shelf life■

Laemmli format, no special buffers required■

Integrated upper buffer chamber

Criterion TGX Precast Gel Specifications
Gel dimensions (W x L)

13.3 x 8.7 cm; 1.0 mm thickCassette dimensions (W x L)

15.0 x 10.6 cm; 5.3 mm thickCassette material

Styrene copolymer

Comb material		

Polycarbonate

Gel storage conditions

Store flat at 4°C; do not freeze

Shelf life at recommended
temperature		

12 months (from date of 		
manufacture)

Recommended sample buffer(dilute 1:1 with sample)
			

Laemmli sample buffer: 62.5 mM
Tris-HCl, pH 6.8, 2% SDS, 25%
glycerol, 0.01% bromophenol blue

Recommended running buffer(Tris/glycine/SDS)

25 mM Tris, 192 mM glycine,
0.1% SDS, pH 8.3

Run times					

42–50 min at 200 V
20–26 min at 300 V

Available Chemistries
IEF

pH 3–10, 5–8

TBE

5%

TBE-urea

5%, 10%, 15%TGX

7.5%, 10%, 12% resolving
4–15%, 4–20%, 8–16% linear gradient
Any kDTGX Stain-Free

7.5%, 10%, 12%, 4–15%, 4–20%
8–16% linear gradient
Any kD

Tris-acetate

3–8% resolving

Tris-HCI

10%, 12.5%, 15%, 4–15%, 4–20%, 10–20%

Tris-Tricine

16.5%

XT (Bis-Tris)

10%, 12%, 4–12% resolving

Criterion TGX Stain-Free Precast Gels
Criterion TGX Stain-Free precast gels combine TGX
formulation with a proprietary compound that facilitates
protein visualization in less than 5 minutes using
Bio-Rad’s Stain-Free enabled imaging systems(see page 284).
Criterion TGX Stain-Free precast gels eliminate the needfor staining. In addition to the 12-month shelf life, Laemmli
buffer system, and fast run times of the TGX formulation,
the Criterion TGX Stain-Free gels provide:
■

■

■

■

 omplete protein separation, gel imaging, and dataC
analysis in 25 min
Comparable sensitivity to Coomassie stain
 etter reproducibility and quantitation compared toBstaining procedures
 se of the same gel for chromatography, westernU
blotting, standard staining methods, and mass
spectrometry analysis

For More Information
Web: bio-rad.com/midistainfree
Download bulletin: 5974

* U.S. patent 6,093,301.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 203

Protein Electrophoresis
Midi-Format 1-D Electrophoresis Systems

Extended Shelf-Life Criterion XT Gels for
SDS-PAGE and Native PAGE
Criterion XT gels are formulated at near-neutral
pH to ensure longer shelf life (12 months for Bis-Tris
gels, 8 months for Tris-acetate gels) and improved
protein stability. Criterion XT gels are run using
optimized sample and running buffers — without theneed for antioxidant addition — for sharp bands andminimal preparation time. Like traditional Laemmlisystems, Criterion XT gels use discontinuous bufferthat forms moving boundaries to stack and thenseparate proteins.

bio-rad.com/midielectro

Criterion XT Migration Charts(with Precision Plus Protein Unstained Standards)
10%

12%

Bis-Tris
4–12%
10%

12%

4–12%

Tris-Acetate
7%

Criterion XT Bis-Tris gels are formulated by using a
Bis-Tris buffer system (pH 6.4) for separation ofproteins by MW. By selecting from two runningbuffers (MOPS or MES) you can expand theseparation capability of a single Bis-Tris gel type.
For More Information
Web: bio-rad.com/criteriongels

Size, kD (XT MES buffer)Size, kD (XT MOPS buffer)

Specifications
Gel dimensions

13.3 x 8.7 cm (W x L); 1.0 mm thickCassette dimensions

15.0 x 10.6 cm (W x L); 5.3 mm thickCassette material

Styrene copolymer

Comb material

Polycarbonate

Storage tray material

PET

Gel storage conditions				Store flat; do not freeze
Room temperature for Bis-Tris gels
4°C for all other gel types

Gel shelf life*
		
		
		
		

12 months for TGX and Bis-Tris gels
8 months for Tris-acetate gels
12 weeks for Tris-HCl, Tris-Tricine,
TBE, TBE-urea gels
26 weeks for IEF gels

Buffer volume

Upper, 60 ml; lower, 400 ml

Size, kD
(XT Tricine buffer)

Criterion Migration Charts(with Precision Plus Protein Unstained Standards)
Tris-HCI Gels
10%

12.5%

15%

4–15%

4–20% 10–20%* From date of manufacture.

204 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

116
97.5

66

97.5

97.5

97.5

116
66
97.5

116
45
66
97.5

31
45
66

116
31
45
97.5

31
21.5
45

66
31
21.5
45
14.4

45
31
14.4

31
21.5
6.5

31
66
45

66
45

14.4
31
21.5
6.5

45

31
45

31
45
21.5

31

31
21.5

31
6.5
21.5

6.5
21.5

14.4

14.4

14.4

21.5
bio-rad.com/midielectro
14.4
14.4
14.4
14.4
6.5
6.5

66
66
116
97.5
45
45
66

66
66

66
66

66

45
45

45
45

45

31
45
31

31

31

31

31

31

21.5
31

21.5

21.5

31
21.5
6.5

31
21.5
21.5

21.5
14.4
21.5

21.5
14.4

21.5
14.4

21.5

14.4
21.5
14.4
6.5

14.4
21.5
14.4
14.4
6.5
6.5
6.5

14.4
6.5
14.4

6.5
14.4

6.5
14.4

14.4

6.5
6.5

6.5

6.5

6.5

116
97.5
116
31
97.5
66

66
116
97.5
116
97.5
45
66

66

66

21.5
45

45
31
45

6.5

31

Protein Electrophoresis

Midi-Format 1-D Electrophoresis Systems

Criterion TGX and TGX Stain-Free Migration Charts™ Unstained™ Unstained™ Unstained
Precision
Plus
ProteinPrecision
Plus
Protein
Plus
ProteinStandards
StandardsStandards(with Precision
Precision
Plus
ProteinUnstained
Standards)
7.5%

7.5%
10%

7.5%
10%
12%

10%
12%
18%

4–15%
18%
12%

12%

4–15%

250

250
250
150

250
150
250
100
250
150
75
100
150

250
250
150
150
100
250
100
75
150
75
50
100
37
50
75

250
150
150
100
100
75

250
150
100
75
250
50
250

37
25
50
20

37
100
25
75
20

25
37

25
50
15
20
37

150

100
150

100

75
100

75

50
75

50

37
50

37

25
20

7.5%

50
75
100
37
50
75
25
37
50
20

10%

15
20

75
50
250

37
50
150

4–15%
4–20%
18%

150
37
150
100
75
100
25
75
20
50
37
50
15
37
25

20

37
25
15
20

25
10
15
20

10
25
15
20

25
15
20

10
25
15
20

10
15

15
10

20
10
25
15
20
10
15

10

10

37
25

4–15%
8–16%
4–20%

10–20%
8–16%
4–20%

10–20%
8–16%
Any kD

10–20%
Any kDAny kD

250
150
250
250
100
150
75
150

250
250
150
150
250
100
100
75
150
75
50
100
37
75
50

250
250
150
150
100
100
75

250
250
150
150
100
100
75

250
150
100

75
50

75
50

75

37
50

50
37

50

37
50
25

37
25
20
25

37
25
20
25

37

15
20

15
20

20

10
15

10
15

15

10

10

10

4–20%

100
75
50
100
75
37
50
37
50
25
37
25
20
20
25
15
15
20
10
15
10
10

8–16%

Any kD

37
20
25
25
15
20
20
15
10
15
10
10

25

Criterion TGX/TGX
CriterionStain-Free
TGX/TGX
Criteriongels.
Stain-Free
TGX/TGX gels.
Stain-Free gels.

Criterion TGX and TGX Stain-Free Migration Charts(with Broad Range Unstained Protein Standards)

Broad Range,
BroadUnstained
Range,
Broad
Unstained
Standards
Range, UnstainedStandardsStandards

7.5%

7.5%
10%

7.5%
10%
12%

18%
10%
12%

4–15%
18%
12%

4–15%
4–20%
18%

200
116
97.5

200
116
200
97.5
200
116
66
97.5

200
200
116
97.5
116
200
66
97.5

200
200
116
97.5
116
66
97.5
200
45
66

200
116
97.5
66
200
200
45

116
66
97.5

116
45
66
97.5

31
45
66

116
31
45
97.5

116
97.5
116
31
97.5
66

66
116
97.5
116
97.5
45
66

31
21.5
45

66
31
21.5

66

66

66
45

31
66
45

21.5
45

45
14.4

45
31
14.4

45
31
45

31
45
21.5

14.4
31
21.5
6.5

200

200

116
97.5

66

45

31

31
45

7.5%

10%

116
45
66
97.5

14.4

14.4

31
21.5

31
6.5
21.5

6.5
21.5

14.4

14.4

14.4

12%

4–15%

IEF

8–16%
4–20%
4–15%

10–20%
8–16%
4–20%

10–20%
Any
8–16%
kD™

10–20%
Any kD™

Any kD™

200
200
116
200
97.5

200
200
200
116
116
97.5
97.5
66
66
116
97.5
45
45
66

200
200
116
116
97.5
97.5
66
66

200
200
116
116
97.5
97.5
66
66

116
97.5

45
45

45
45

31
45
31

31

31

31

31

31

21.5
31

21.5

21.5

4–20%

8–16%

Any kD

31
21.5
21.5

21.5
14.4
21.5

21.5
14.4

21.5
14.4

21.5

14.4
21.5
14.4
14.4
6.5
6.5
6.5

14.4
6.5
14.4
6.5
6.5

6.5
14.4

6.5
14.4

14.4

6.5

6.5

6.5

7.5%
10%
12%

10%
18%
12%

4–15%
18%
12%

4–15%
4–20%
18%

250

250
250
150

250
150
150
100
250
100
75
150

250
150
150
100
100
75

150

100
150

250
150
250
100
250
150
75
100
150

75
50
250

100

75
100

75
50
100
37
50
75

250
150
100
75
250
50
250

75

50
75

37
25
50
20

37
100
25
75
20

25
37

50

37
50

25
50
15
20
37

37

25
20

25
37
50
20

37
50
150

150
37
150
100
75
100
25
75
20
50

pH
(IEF cathode buffer,
IEF anode buffer)

37
50
15
37
25

4–15%
4–20%
8–16%

10–20%
4–20%
8–16%

10–20%
8–16%
Any kD

10–20%
Any kDAny kD

250
150
250
250
100
150
75
150
100
75
50
100

250
250
150
150
250
100
100
75
150
75
50
100
37
75
50

250
250
150
150
100
100
75

250
250
150
150
100
100
75

250
150
100

75
50

75
50

75

50
37

50
37

50

37
50
25

37
25
20
25

37
25
20
25

37

15
20

15
20

20

10
15

10
15

15

10

10

10

75
37
50

37
50
15
25
20
37
25
20
20
20
37
37
25
25
25
10
10
10
25
25
25
15
15
15
15
15
20
20
20
15
20
20
20
10
10
In the U.S.,
call toll
1-800-424-6723
15
15
15
10
10 free at10
25
25
10
15
15
15
20
20
10
10
10

37
20
25
25
15
20
20
15
10
15
10
10

5%

10%

15%

31

31
21.5
6.5

7.5%
10%

5%

45

14.4
21.5
14.4
6.5

7.5%

TBE-Urea

TBE

66

31
21.5
6.5

6.5

pH 5–8

200

21.5
14.4
14.4
6.5
6.5

™ Unstained™ Unstained™ Unstained
Precision Precision
Plus Protein
Precision
Plus
Protein
Plus
ProteinStandards
StandardsStandards

50
75
100
37
50
75

pH 3–10Size, bp
(TBE buffer)Size, bp
(TBE buffer)

XC, xylene cyanole;
BPB, bromophenol
blue (tracking dyes)

25Electrophoresis and Blotting | 205

Protein Electrophoresis
Midi-Format 1-D Electrophoresis Systems

bio-rad.com/midielectro

Ordering Information

12+2-Well*,**45 μl

18-Well
50 μl

26-Well*
15 μl

Prep+2-Well**
800 μl

Criterion TGX Precast Gels
7.5% Resolving Gel
10% Resolving Gel
12% Resolving Gel
4–15% Linear Gradient
4–20% Linear Gradient
8–16% Linear Gradient
Any kD Gel

56710235671033
5671043
5671083
56710935671103
56711235671024
5671034
5671044
5671084
5671094
5671104
5671124

5671025
5671035
5671045
5671085
5671095
5671105
5671125———
5671082
5671092
5671102
5671122———56710815671091
5671101
5671121

15.00
15.00
15.00
15.00
15.00
15.00
15.00

Criterion TGX Stain-Free Precast Gels
7.5% Gel
10% Gel
12% Gel
4–15% Gel
4–20% Gel
8–16% Linear Gradient
Any kD Gel

56780235678033
5678043
5678083
56780935678103567812356780245678034
56780445678084
56780945678104
567812456780255678035
56780455678085
567809556781055678125———5678082567809256781025678122———567808156780915678101
5678121

16.00
16.00
16.00
16.00
16.00
16.00
16.00

Criterion XT Bis-Tris Gels***
10% Resolving Gel
12% Resolving Gel
4–12% Resolving Gel

345011134501173450123345011234501183450124

345011334501193450125——
3450126†

345011534501213450127

16.00
16.00
16.00

Criterion XT Tris-Acetate Gels
3–8% Resolving Gel

345012934501303450131

—

—

16.00

Criterion Tris-HCl Gels
10% Resolving Gel
12.5% Resolving Gel
15% Resolving Gel
4–15% Linear Gradient
4–20% Linear Gradient
10–20% Linear Gradient

34500093450014345001934500273450032345004234500103450015345002034500283450033
3450043

345001134500163450021345002934500343450044

—
—
—
—
—
—

3450101
3450102——3450104
3450107

15.00
15.00
15.00
15.00
15.00
15.00

Criterion Tris-Tricine Gels
16.5% Tris-Tricine

3450063

34500643450065†

—

—

16.00

Criterion IEF Gels
pH 3–10pH 5–83450071†—3450072†
3450076†

—
—

—
—

—
—

38.00
57.00

Criterion TBE Gels
5% TBE Gel

3450047

34500483450049

—

—

16.00

Criterion TBE-Urea Gels
5% TBE-Urea Gel
10% TBE-Urea Gel
15% TBE-Urea Gel—
3450088†
3450091

3450086†
3450089†
3450092—
3450090†3450093†

—
—
—

—
—
—

19.00
16.00
16.00

Criterion Empty Cassettes
1.0 mm thick, 10 sets

3459901

345990234599033459904

3459906

81.00

—

—

1656008

—

—

27.00

Description

Loading Guides
Criterion Sample Loading Guide††

IPG+1-Well**
11 cm IPG Strip Price, U.S. $continues

continues

206 | Electrophoresis and Blotting

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Protein Electrophoresis
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Catalog #Midi-Format 1-D Electrophoresis Systems

Description

Criterion XT Buffers and Reagents
1610788***
XT MOPS Running Buffer, 20x, 500 ml
1610789***
XT MES Running Buffer, 20x, 500 ml
1610790
XT Tricine Running Buffer, 20x, 500 ml
1610791
XT Sample Buffer, 4x, 10 ml
1610792
XT Reducing Agent, 20x, 1 ml
XT MOPS Buffer Kit, includes 500 ml of 20x XT MOPS running buffer, 10 ml of 4x XT sample buffer,
1610793***
1 ml of 20x XT reducing agent
XT MES Buffer Kit, includes 500 ml of 20x XT MES running buffer, 10 ml of 4x XT sample buffer,
1610796***
1 ml of 20x XT reducing agent
1610797
XT Tricine Buffer Kit, includes 500 ml of 20x XT Tricine running buffer, 10 ml of 4x XT sample buffer,
1 ml of 20x XT reducing agent
Application Guide
4110001
Criterion Precast Gel Application Guide, available online

Price, U.S. $
63.00
63.00
63.00
13.00
23.00
96.00

See Also
Premixed runningbuffers: page 214.

96.00
96.00

Inquire*  Multichannel pipet compatible.
** Includes reference well(s), 15 μl.
*** Purchase of this product is accompanied by a limited license under U.S. patents 6,143,154; 6,096,182; 6,059,948; 5,578,180;
5,922,185; 6,162,338; and 6,783,651 and corresponding foreign patents.
† Please allow up to 2 weeks for delivery.
††
U.S. patent 5,656,145.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 207

Protein Electrophoresis
Midi-Format 1-D Electrophoresis Systems

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Criterion Accessories
Empty Cassettes
Single-use empty Criterion cassettes are available for handcasting gels. For added convenience, cast your gels using
AnyGel stands.
For More Information
Download bulletins: 2710, 2911, and 2912

AnyGel Stands
AnyGel stands are convenient for storing glass plates of any
size gel. They are available as single-row or six-row stands.
See the Mini-PROTEAN precast gel section, page 196.

Criterion Staining Trays
Criterion staining/blotting trays are plastic trays designed
specifically for staining one or two Criterion gels or
performing western blot detection. Tray dimensions are
optimized for Criterion gel staining and for blot detection.
These dimensions provide a working volume of up to 500 ml.
* U.S. patent 6,843,593.

Ordering Information
Catalog #Description

Price, U.S. $AnyGel Stands and Accessories
1654131
AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Mini-PROTEAN mini gels
1655131
AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Mini-PROTEAN gels
1654132
Replacement Clamps, 278.00
295.0033.00

Criterion Staining Trays
3459921
Criterion Staining/Blotting Trays, with lids, 23459920
Criterion Staining/Blotting Trays, with lids, 1225.00
215.00

Dodeca Stainers, Accessories, and Replacement Parts
1653416
Dodeca Stainer Tray, small, replacement, 21653420
Dodeca Stainer White Development Tray, small
1653422
Dodeca Stainer Shaking Rack, small, replacement
1653423
Dodeca Stainer Solution Tank, large, replacement
1653424
Dodeca Stainer Solution Tank, small, replacement
1653425
Dodeca Stainer Lid with Shaker Motor, 100–240 V, replacement, fits both tank sizes
1653426
Dodeca Stainer Lid without Shaker Motor, replacement, fits both tank sizes
1653428
Dodeca Stainer Shaker Control Unit, replacement

125.0045.00
925.00420.00360.00950.00180.00280.00208 | Electrophoresis and Blotting

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Protein Electrophoresis
Large-Format Vertical Electrophoresis Systems

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See Also

PROTEAN II xi and XL Accessories
Accessories and replacement parts are available for the
PROTEAN II xi and XL systems. There are a wide varietyof glass plates, spacers, and combs to choose from.
Components can be purchased separately for a truly
customized system. Replacement parts are also available
to keep your system up and running.

1

xi

xi

2

PowerPac Universal
power supply:
page 186.

XL
4

XL

Premixed buffers
and buffer reagents:
page 245.
Dodeca stainers:
page 208.

xi

For More Information
Web: bio-rad.com/largeelectro
Download bulletin: 1760

3
XL

xi

XL

PROTEAN II xi and XL (IPG) component comparison:
1. The 4 mm xi and 13 mm XL clamp notches.
2. The 181 mm xi and 198 mm XL central cooling core gaskets.
3. The 153 mm xi and 184 mm XL combs.
4. The 19 mm xi and 8 mm XL spacers.

Maximum Sample Volume per Well for PROTEAN II xi and XL Combs*						

Comb Thickness

Number or Type of Wells

Well Width

0.5 mm0.75 mm

1.0 mm

1.5 mm

3.0 mm

25		
20		
15		
10		
5		
3		
Blank
2-D (IPG well)		 Reference well
		 Sample well

3.5 mm
5.0 mm
6.5 mm
1.0 cm2.3 cm
4.0 cm14.5 cm

—
54 µl
74 µl
114 µl
—
—
—

60 µl
82 µl
110 µl172 µl——2.44 ml

80 µl
110 µl147 µl229 µl522 µl—3.26 ml

120 µl164 µl221 µl343 µl783 µl1.37 ml
4.88 ml—
328 µl442 µl687 µl1.57 ml—9.76 ml

3.5 mm
17.8–17.9 cm

—
—

—
—

28 µl
—

42 µl
—

84 µl
—

* At standard 25 mm well depth.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 209Protein Electrophoresis
Large-Format Vertical Electrophoresis Systems

bio-rad.com/largeelectro

Ordering Information
Catalog #Description

Price, U.S. $PROTEAN II xi Accessories for Running Gels
1651901
PROTEAN II xi Sandwich Clamps, 16 cm set (1 left, 1 right)
1651902
PROTEAN II xi Sandwich Clamps, 20 cm set (1 left, 1 right)
1651913
PROTEAN II xi Replacement Gaskets, for central cooling core, 2135.00135.0059.00

PROTEAN II XL Accessories for Running Gels
1651835
PROTEAN II XL Sandwich Clamps, IPG set (1 left, 1 right)
1653182
PROTEAN II XL Replacement Gaskets, for central cooling core, 2

140.0071.00

PROTEAN II xi and XL Accessories for Running Gels
1651806
Central Cooling Core, includes 2 gaskets
1651808
Cell Lid, with power cables
1651909
Upper Buffer Dam
1005430
PROTEAN II Latch Assembly Kit, for central cooling core

980.00245.0092.00
51.00

PROTEAN II xi and XL Casting Apparatus
1651911Slab Gel Casting Stand, with gaskets
1651912
Replacement Gaskets, for casting stand, 2

225.0044.00

PROTEAN II xi Glass Plates*
1651821
Inner Plates, for 16 x 16 cm gels, 16 x 20 cm, 2, for PROTEAN II xi cell only
1651822
Outer Plates, for 16 x 16 cm gels, 18.3 x 20 cm, 2, for PROTEAN II xi cell only

58.00
58.00

PROTEAN II xi Specialty Glass Plates
1651826**
Frosted Inner Plates, for agarose gels, 20 x 20 cm, 2, for PROTEAN II xi cell only
1651832
Notched Inner Plate, for double-up procedures, 16 x 16 cm gel, 16 cm bevel length, for PROTEAN II xi cell only
1651833
Notched Inner Plate, for double-up procedures, 20 x 20 cm gel, 16 cm bevel length, for PROTEAN II xi cell only

145.00120.00120.00PROTEAN II xi and XL Glass Plates, for 16 x 20 or 18.3 x 20 cm gels*
1651823
Inner Plates, 20 x 20 cm, 21651824
Outer Plates, 22.3 x 20 cm, 2

58.00
58.00

Spacer Width

0.5 mm0.75 mm

1.0 mm

1.5 mm

3.0 mmPrice, U.S. $PROTEAN II xi Spacers (Set of 4)
16 cm gels
20 cm gels

1651841
165184616518421651847

1651843
165184816518441651849

16518451651850

91.00
91.00

PROTEAN II xi Combs**
Blank
2-D IPG
3-Well5-Well
10-Well
15-Well
20-Well
25-Well————
165187516518701651865—1651891———165187616518711651866
165186116518921651897—1651882165187716518721651867165186216518931651898
16518881651883165187816518731651868165186316518941651899—
1651884165187916518741651869
—

64.00
64.00
64.00
64.00
64.00
64.00
64.00
64.00

Spacer Width

1.0 mm

1.5 mm

2.0 mmPrice, U.S. $PROTEAN II XL Combs, IPG Strip Format
2-D IPG (with reference well)

1651838

1653187

1651839

71.00

Catalog #Description

PROTEAN II XL Spacers, IPG Strip Format (Set of 4)1651836
20 cm Spacers, 1.0 mm
1653181
20 cm Spacers, 1.5 mm
1651837
20 cm Spacers, 2.0 mm

98.00
110.00100.00*		One gel sandwich consists of 1 outer plate, 1 inner plate, and 2 spacers.
** Each comb is 15.2 cm long. All combs except the 2-D combs produce sample wells that are 25 mm deep.
The well depth of the 2-D comb is 8 mm. The well depth of all standard combs can be converted from 25 mm to
10 mm with comb conversion screws (#1651859).

210 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
bio-rad.com/largeelectro

Large-Format Vertical Electrophoresis Systems

See Also

PROTEAN II Multi-Gel Casting Chamber
■

■

■

■

■

Acrylamide
gel-casting reagents:
page 217.

 p to twelve 1.5 mm thick gels can be castUsimultaneously

Premixed buffers:
page 214.

Top filling for uniform single percentage gels
 ottom filling for reproducible gradient gels using theB
Model 495 gradient former (see page 212)
Accommodation for 16 or 20 cm gels
 crylic blocks can be used as space fillers when fewerAthan 10 gels are cast, and reusable separation sheets
offer easy separation of gel sandwiches after casting

Ordering Information
Catalog #Description

1652025

 ROTEAN II xi Multi-Gel Casting Chamber, includes casting chamber, sealing plate, silicone gasket,
P
15 separation sheets, 4 acrylic blocks, 10 xi alignment cards, tapered luer connector, leveling bubble
PROTEAN II XL Multi-Gel Casting Chamber, includes casting chamber, sealing plate, silicone gasket,
15 separation sheets, 4 acrylic blocks, 10 XL alignment cards, tapered luer connector, leveling bubble

1652024
Accessories
1651957
1651958
16520261652029
1651840

Acrylic Blocks, 4
Separation Sheets, 15
Sealing Gaskets, 3
PROTEAN II xi Alignment Cards, 2
PROTEAN II XL Alignment Cards, 2

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $765.00865.00135.00120.00
63.00
22.00
58.00

Electrophoresis and Blotting | 211

Protein Electrophoresis
Large-Format Vertical Electrophoresis Systems

See Also
Acrylamide
gel-casting reagents:
page 217.
Premixed buffers:
page 214.

bio-rad.com/largeelectro

Large-Format Electrophoresis Accessories
PROTEAN Plus Combs and Hinged Spacer Plates
PROTEAN Plus hinged spacer plates are two glass plates
joined together by a silicone hinge with integrated spacers
bonded onto the long plate to guarantee perfect alignment
during casting and eliminate potential current leaks. The
differential plate heights facilitate easy IPG strip or tube gelloading. The hinged spacer plate sizes are the same for both
gel sizes (20 and 25 cm wide). Combs are available for both 20
and 25 cm wide gel sizes and 1.0, 1.5, or 2.0 mm thick gels.
PROTEAN Plus Multi-Casting Chamber
Use the PROTEAN Plus multi-casting chamber to cast up
to 12 gels of 1.0, 1.5, or 2.0 mm thickness simultaneously.
The chamber accommodates the PROTEAN Plus hinged
spacer plates for both 20 and 25 cm wide gel sizes.
Acrylic blocks act as space fillers when fewer than 12 gelsare cast, and a leveling bubble ensures level interfaces.
Gradient gels are cast through a bottom port using theModel 495 gradient former (below).

Hinged Spacer Plates

PROTEAN Plus MultiCasting Chamber

Model 495 Gradient Former
This gradient former allows you to pour linear or convex
exponential acrylamide gradients. Its 100–1,500 ml
capacity is designed to pour up to 12 gradient slab gels in
the PROTEAN Plus multi-casting chamber. The optional
exponential piston is required to form convex exponential
acrylamide gradients.
AnyGel Stands
AnyGel stands provide stabilization and access to virtually
any size gel. The clamping mechanism secures gel
cassettes vertically without excessive pressure.
Gel Clip
The gel clip facilitates the handling of large-format gelsand eliminates gel breakage by minimizing direct hands-on
gel manipulation. The gel clip gently but securely clamps
along one edge of a gel, distributing the weight evenly sothat the gel can be easily lifted without tearing.

212 | Electrophoresis and Blotting

Using the gel clip to clamp onto a gel(PROTEAN Plus precast gel shown).

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
bio-rad.com/largeelectro

Large-Format Vertical Electrophoresis Systems

Ordering Information
Catalog #Description

Price, U.S. $PROTEAN Plus Combs
1654176
2-D Comb with 1 Reference Well, 20 cm, 1.0 mm
1654177
2-D Comb with 1 Reference Well, 20 cm, 1.5 mm
1654179
2-D Comb with 1 Reference Well, 25 cm, 1.0 mm
1654181
2-D Comb with 1 Reference Well, 25 cm, 2.0 mm

25.00
25.00
25.00
25.00

PROTEAN Plus Hinged Spacer Plates
1654170
Hinged Spacer Plates, for 20 x 20.5 cm gels, 1.0 mm, 1 set
1654171
Hinged Spacer Plates, for 20 x 20.5 cm gels, 1.5 mm, 1 set

110.00110.00Accessories for PROTEAN Plus Multi-Casting Chamber
1654162
Acrylic Block, 3 mm
1653320
Gel Releasers, 5

41.00
30.00

Gel Clip
165341441.00

Gel Clip, holds any gel size

Model 495 Gradient Former
1654121
Model 495 Gradient Former, 100–1,500 ml, includes body with valve stem and tubing connection kit
Model 495 Gradient Former and PROTEAN Plus Multi-Casting Chamber, includes #1654121 and #1654160
1654123

1,150.00
2,165.00

Accessories for Model 495 Gradient Former
1652008
Tubing Connection Kit, includes stopcock, luer taper coupling, tubing (1/8" ID, 3'), Y-connector

44.00

PROTEAN II xi Plate Washer/Holder
1651992*
PROTEAN II xi Plate Holder
1610722
Bio-Rad Cleaning Concentrate, 50x, 1 kg

155.0059.00* Not compatible with Mini-PROTEAN 3 short plates and spacer plates or with PROTEAN Plus hinged spacer plates.
Accessories and Replacement Parts for PROTEAN Plus Dodeca Cell
1654154
Replacement Gasket Assembly
1654155
Replacement Electrode Card, anode
1654156
Replacement Electrode Card, cathode1654167
Buffer Exhaust Tubing
1653320
Gel Releasers, 51652948
Replacement Power Cables, for lid

In the U.S., call toll free at 1-800-424-6723

160.001,035.00
285.00
82.00
30.00
73.00

Electrophoresis and Blotting | 213

Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis

bio-rad.com/proteinreagents

Buffers and Reagents for Protein Electrophoresis
See Also
ReadyPrep 2-D
samplepreparation kit:
page 4.
ReadyStrip IPG
strips: page 225.

Premixed Sample Loading Buffers
The concentrated formulas of these buffers allow them
to be used with both liquid and lyophilized samples.
All premixed sample buffers are tested to ensure quality
and consistency.
For More Information
Web: bio-rad.com/proteinreagents
Premixed Sample Loading Buffer Selection Guide
BufferFormulation

Applications

Laemmli sample buffer (2x)		62.5 mM Tris-HCI, pH 6.8, 2% SDS, 20% glycerol (w/v),
0.01% bromophenol blue (BPB)

SDS-PAGE

Laemmli sample buffer (4x)		

250 mM Tris-HCI, pH 6.8, 4% LDS, 40% glycerol (v/v),
0.02% bromophenol blue (BPB)

SDS-PAGE

Native sample buffer

62.5 mM Tris-HCI, pH 6.8, 40% glycerol (w/v), 0.01% BPB

Native PAGE

Tricine sample buffer		

200 mM Tris-HCI, pH 6.8, 2% SDS, 40% glycerol (w/v),
0.04% Coomassie Brilliant Blue G-250

Peptide and small protein SDS-PAGE

IEF sample buffer

50% glycerol (v/v)

IEF

Ordering Information
Catalog #Description

Premixed Protein Sample Loading Buffers
1610737
2x Laemmli Sample Buffer, 30 ml
1610747
4x Laemmli Sample Buffer, 10 ml
1610738
Native Sample Buffer, 30 ml
1610739
Tricine Sample Buffer, 30 ml
1610763
IEF Sample Buffer, 30 ml
1610791
XT Sample Buffer, 4x, 10 ml

214 | Electrophoresis and Blotting

Price, U.S. $
14.00
15.00
16.00
14.00
14.00
13.00

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis

bio-rad.com/proteinreagents

See Also

Premixed Running Buffers

Criterion XT buffers
and reagents:
page 207.

Premixed running buffers can be used with handcast or
precast gels. Simply dilute with distilled deionized water.
For running buffers designed especially for extended shelf
life Criterion XT precast gels, see page 207.

Electrophoresis Running Buffer Selection Guide
Buffer

1x Formulation

Applications

Protein Electrophoresis
10x Tris/glycine/SDS		

25 mM Tris, 192 mM glycine,
0.1% SDS, pH 8.3

General SDS-PAGE

10x Tris/glycine		

25 mM Tris, 192 mM glycine,pH 8.3Native PAGE

10x Tris/Tricine/SDS		

100 mM Tris, 100 mM tricine,
0.1% SDS, pH 8.3

Peptide SDS-PAGE

10x IEF anode buffer

7 mM phosphoric acid

Analytical isoelectric focusing

10x IEF cathode buffer

20 mM lysine, 20 mM arginine

Analytical isoelectric focusing

10x TBE		

89 mM Tris, 89 mM boric acid,
2 mM EDTA, pH 8.3

Nucleic acid electrophoresis/sequencing;polyacrylamide or agarose gels

50x TAE		

40 mM Tris, 20 mM acetic acid,
1 mM EDTA, pH 8.0

Nucleic acid electrophoresis;
polyacrylamide or agarose gels

Nucleic Acid Electrophoresis

Ordering Information
Catalog #Description

Premixed Protein Running Buffers
1610732
10x Tris/Glycine/SDS, 1 L
1610772
10x Tris/Glycine/SDS, 5 L cube
1610734
10x Tris/Glycine, 1 L
1610771
10x Tris/Glycine, 5 L cube
1610744
10x Tris/Tricine/SDS, 1 L
1610761
10x IEF Anode Buffer, 250 ml
1610762
10x IEF Cathode Buffer, 250 ml

Price, U.S. $
28.00
94.00
28.00
94.00
86.00
11.00
57.00

Catalog #Description

Price, U.S. $Premixed Nucleic Acid Running Buffers
1610733
10x Tris/Boric Acid/EDTA (TBE),
1L
1610770
10x Tris/Boric Acid/EDTA (TBE),
5 L cube
1610743
50x Tris/Acetic Acid/EDTA (TAE),
1L

37.00
110.0057.00
84.00

See Also

Premixed Gel-Casting Buffers
Tris-HCl buffers are available to prepare the stacking and resolving portions of native or SDS-PAGE gels using
discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Use the 0.5 M Tris-HCl, pH 6.8,
buffer for stacking gels and the 1.5 M Tris-HCl, pH 8.8, buffer for resolving gels.

ReadyStrip IPG
strips: page 225.
Vertical
electrophoresis
systems: page 192.
Horizontal
electrophoresis
systems: page 254.

Ordering Information
Catalog #Description

1610798
1610799

Resolving Gel Buffer, 1.5 M Tris-HCI, pH 8.8, 1 L
Stacking Gel Buffer, 0.5 M Tris-HCI, pH 6.8, 1 L

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
28.00
28.00

Electrophoresis and Blotting | 215

Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis

bio-rad.com/proteinreagentsTGX Stain-Free Solutions
See Also
Mini-PROTEANTGX precast gels:page 196.
Mini-PROTEANTGX Stain-Free
precast gels:page 196.

Video
Product
Demonstration

bio-rad.com/
TGXcastingTGX Handcast Acrylamide Solutions
TGX Stain-Free FastCast Acrylamide Solutions
TGX Stain-Free FastCast acrylamide solutions are readyto-use solutions for hand casting polyacrylamide gels for
SDS-PAGE or PAGE. Stain-Free technology eliminates
extra steps by confirming electrophoresis results andtransfer performance before western blotting, conserving
precious samples and reducing waste. Each kit comes with
two resolver and stacker solutions, which are each mixed
1:1 with the appropriate amount of TEMED and APS.
TGX FastCast Acrylamide Solutions
TGX FastCast acrylamide solutions are ready-to-use
solutions for hand casting polyacrylamide gels for
SDS-PAGE or PAGE. Each kit comes with two resolver
and stacker solutions, which are each mixed 1:1 withthe appropriate amount of TEMED and APS.

 ast blotting times — efficient protein transfers in asFlittle as 3 min using the Trans-Blot Turbo transfer system■

Advantages of the TGX and TGX Stain-Free FastCast
acrylamide kits:

 tain-Free technology — for fast imaging or for better,S
more reliable total protein normalization in western
blotting (using Stain-Free FastCast kits and Bio-Rad
Stain-Free enabled imagers)

■
■

■

■

 aster casting times — ability to pour stacker
F
immediately after resolver ong shelf life gels — gels can be used up toL
1 month after casting when stored at 4°C
Fast run times — run times of as little as 20 min

For More Information
Web: bio-rad.com/tgxfastcast
bio-rad.com/tgxstainfreefastcast

Approximate Number of Gels per Kit
Mini Gels (~9 x 7 cm)
1.0 mmMini-PROTEAN Cassettes

0.75 mm
Glass Plates

Midi Gels (~13 x 9 cm)

1.0 mmGlass Plates

1.5 mmGlass Plates

1.0 mmCriterion Cassettes

Starter Kit

11 gels

19 gels

13 gels

9 gels

6 gels11 gels

55 gels

95 gels

65 gels

45 gels

30 gels

Ordering Information
Catalog #Description

161017016101711610172161017316101741610175
161018016101811610182
1610183
1610184
1610185

 GX FastCast Acrylamide Starter Kit, 7.5%
T
TGX FastCast Acrylamide Kit, 7.5%
TGX FastCast Acrylamide Starter Kit, 10%
TGX FastCast Acrylamide Kit, 10%
TGX FastCast Acrylamide Starter Kit, 12%
TGX FastCast Acrylamide Kit, 12%
TGX Stain-Free FastCast Acrylamide Starter Kit, 7.5%
TGX Stain-Free FastCast Acrylamide Kit, 7.5%
TGX Stain-Free FastCast Acrylamide Starter Kit, 10%
TGX Stain-Free FastCast Acrylamide Kit, 10%
TGX Stain-Free FastCast Acrylamide Starter Kit, 12%
TGX Stain-Free FastCast Acrylamide Kit, 12%

216 | Electrophoresis and Blotting

Price, U.S. $50.00
180.0050.00
180.0050.00
180.0055.00
200.0055.00
200.0055.00
200.00In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis

bio-rad.com/proteinreagents

Gel-Casting Reagents
Acrylamide
Acrylamide powders — acrylamide and bis-acrylamide
powders allow adjustment of concentration andacrylamide/bis ratio■■■

Premixed acrylamide/bis powders — premeasured
amounts allow stock solutions from 30–50% to beprepared directly in the bottle by adding the indicatedamount of water crylamide solutions — ready to use and available
Ain two concentrations (30% and 40%) and in threeacrylamide/bis ratios (19:1, 29:1, and 37.5:1)

Pore Size Determination: %T and %C
Polyacrylamide gels are described in terms of two parametersthat determine pore size: total monomer concentration (%T)
and weight percentage of cross-linker (%C).
%T =
%C =

grams acrylamide + grams crosslinker
total volume, mlgrams crosslinker
grams acrylamide + grams crosslinker

x 100%
x 100%

By varying these two parameters, the pore size of the gel canbe optimized to give the best separation and resolution forAcrylamide/Crosslinker Ratio by Application
Ratio

%C

Common Applications

19:1

5

DNA sequencing

29:1

3.3

Protein separation

37.5:1

2.6

Protein separation

the molecule of interest. For help in determining the best %T and%C for your application, refer to the Precast Gels sections on
pages 196 and 202–207. Examples of migration patterns
of proteins on gels of different compositions can be found on
pages 197 (Mini-PROTEAN TGX precast gels) and 204–205(Criterion precast gels), or contact Bio-Rad Technical Support.
For More Information
Web: bio-rad.com/acrylamide
Download bulletins: 1156 and 1866

Ordering Information
Description

Price, U.S. $Acrylamide Solutions*

500 ml

30% acrylamide/bis, 19:1
30% acrylamide/bis, 29:1
30% acrylamide/bis, 37.5:1
40% acrylamide/bis, 19:1
40% acrylamide/bis, 29:1
40% acrylamide/bis, 37.5:1
40% acrylamide
2% bis solution

161015416101561610158
16101441610146
161014816101401610142

Catalog #Price, U.S. $2 x 500 ml

59.00
59.00
59.00
71.00
71.00
71.00
51.00
53.00

—
1610157
1610159
16101451610147
1610149—
1610143120.00120.00120.00135.00135.00135.00105.0094.00

Description

Price, U.S. $Acrylamide Powder
1610100
Acrylamide, 99.9%, 100 g
1610101
Acrylamide, 99.9%, 500 g
1610107
Acrylamide, 99.9%, 1 kg

40.00
160.00285.00Premixed Acrylamide/Bis Powder
1610125

Acrylamide/Bis Powder, 150 g, 37.5:1

Catalog #Description

Price, U.S. $1,200.00

1632091
ReadyPrep Proteomics Grade Water, 500 ml* Store acrylamide solutions at 4°C. All other reagents should be stored at room temperature, dry, and away from direct sunlight.

In the U.S., call toll free at 1-800-424-6723

40.00

Electrophoresis and Blotting | 217

Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis

bio-rad.com/proteinreagents

Crosslinkers and Catalysts
Bio-Rad offers standard and alternative crosslinkers for a variety of applications.
Crosslinker Application Guide		Formal Name

Bis		

N,N'-methylene-		
bis-acrylamide

Chemical Structure

Applications
General crosslinker in PAGE

Ordering Information
Catalog #Description

Crosslinkers
1610201
16101421610143

Bis Crosslinker, 50 g
2% Bis Solution, 500 ml
2% Bis Solution, 2 x 500 ml

Price, U.S. $
51.00
53.00
94.00

Catalysts
1610800
TEMED, 5 ml (hazardous shipping charges may apply)
1610801
TEMED, 50 ml
1610700*
Ammonium Persulfate (APS), 10 g
* For longer shelf life, store desiccated at room temperature.

17.00
55.00
14.00

IEP/IEF Agaroses
Standard low –mr agarose — with high strength andclarity and low –mr value, this agarose is recommended forall standard immunoelectrophoresis applications.

Zero –mr agarose — this agarose is specific for IEF. It has
no detectable electroendosmosis and is recommended for
IEF of very high MW proteins or complexes that are subject
to varying degrees of molecular sieving in polyacrylamidegels. Agarose IEF and post-run processing can becompleted more quickly than with polyacrylamide gel IEF.
For More Information
Web: bio-rad.com/IEP/IEFAgaroses

Ordering Information
Catalog #Description

1620100
1620102
1620022

Standard Low –mr Agarose, 100 g
Standard Low –mr Agarose, 500 g
Zero –mr Agarose, 10 g

Price, U.S. $165.00620.00160.00

All reagents should be stored at room temperature, dry, and away from direct sunlight.

218 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
bio-rad.com/proteinreagents

Buffers and Reagents for Protein Electrophoresis

Accessory Reagents
Bromophenol Blue Tracking Dye
Bio-Rad offers bromophenol blue tracking dye to monitor protein electrophoresis runs.
Ordering Information
Catalog #Description

1610404
Bromophenol Blue, 10 g
Store at room temperature, dry, and away from direct sunlight.

Price, U.S. $40.00

See Also

Detergents
SDS is available in a powder form or as 10% and 20% solutions. They are prepared with 18 MW water and have no detectable
DNase or RNase activity. Also available are Tween 20 for blotting solutions and Triton X-100 and CHAPS for membrane protein
solubilization. For simple, accurate pipetting, a solution of 10% Tween 20 is available.

ReadyPrep
2-D sample
preparation kits:
page 2.

Ordering Information
Catalog #Description

1610301
161030216104161610418
170653116104071610460*
1610781

SDS (Sodium Dodecyl Sulfate), 100 g
SDS (Sodium Dodecyl Sulfate), 1 kg
SDS Solution, 10% (w/v), 250 ml
SDS Solution, 20% (w/v), 1 L
Tween 20, EIA grade, 100 ml
Triton X-100 Detergent, 500 ml
CHAPS, 1 g
10% Tween 20, for easy pipetting, 1 L

Price, U.S. $33.00
210.0045.00
120.00
53.00
35.00
60.00
36.00

* Store desiccated at 4°C. All other reagents should be stored at room temperature, dry, and away from direct sunlight.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 219

Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis

Bio-Lyte Ampholytes
Bio-Lyte carrier ampholytes, supplied as aqueous solutions,are blended to give a complete range of isoelectric points
for linear pH gradients. Bio-Lyte 3/10 and 3/5 ampholytes
extend from approximately pH 3.5 to 9.5 units on the acidic
and basic ends. All other ranges are within 0.1–0.2 pH
units of their specified range. Bio-Lyte ampholytes are also
used as IPG buffers. For ready-to-use ampholytes, see
ReadyStrip IEF buffers (page 225).

pH Range 3

4

5

6

4

5

6

7

8

9

10

7

8

9

10

3/10
3/10
Bio-Lyte ampholyte

See Also
ReadyStrip IPG strips:
page 225.

bio-rad.com/proteinreagents

3/5
3/5
4/6
4/6
5/7
5/7
5/8
5/8
6/8
6/8
7/9
7/9
3

pH

pH range of each Bio-Lyte ampholyte.

Ordering Information
Catalog #Description

163111216311131631132
1631142
1631143
1631152
1631153

Bio-Lyte 3/10 Ampholyte, 40%, 10 ml
Bio-Lyte 3/10 Ampholyte, 40%, 25 ml
Bio-Lyte 3/5 Ampholyte, 20%, 10 ml
Bio-Lyte 4/6 Ampholyte, 40%, 10 ml
Bio-Lyte 4/6 Ampholyte, 40%, 25 ml
Bio-Lyte 5/7 Ampholyte, 40%, 10 ml
Bio-Lyte 5/7 Ampholyte, 40%, 25 ml

Price, U.S. $Catalog #Description

275.00645.00275.00275.00645.00275.00645.001631192
16311931631162
1631163

Bio-Lyte 5/8 Ampholyte, 40%, 10 ml
Bio-Lyte 5/8 Ampholyte, 40%, 25 ml
Bio-Lyte 6/8 Ampholyte, 40%, 10 ml
Bio-Lyte 6/8 Ampholyte, 40%, 25 ml

Price, U.S. $275.00645.00275.00645.00275.00275.00Cleaning Concentrate
Bio-Rad’s cleaning concentrate is a moderately alkaline detergent that cleans by solubilization and emulsification.
It is ideal for cleaning glass plates and other laboratory equipment and is harmless to skin and clothing.
Ordering Information
Catalog #Description

1610722

Bio-Rad Cleaning Concentrate, 50x, 1 kg

Price, U.S. $59.00

Gel Drying Solution
Bio-Rad’s gel drying solution is a pretreatment for polyacrylamide gels that helps prevent gels from cracking during air or
vacuum drying. Just 10 minutes of equilibration in the solution before drying prevents excessive gel swelling and cracking.
Ordering Information
Catalog #Description

1610752

Gel Drying Solution, 1 L

220 | Electrophoresis and Blotting

Price, U.S. $46.00

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Protein Stains

bio-rad.com/proteinstains

Protein Stains
Bio-Rad offers visible and fluorescent gel stains to accommodate your needs for sensitivity, linearity, and mass
spectrometry compatibility.
Gel Stain Selection Guide
Stain

Staining per Band Time

Coomassie Stains
QC Colloidal Coomassie

Comments

3.0 ng

1–20 hr Colloidal endpoint stain; nonhazardous formulation

Bio-Safe Coomassie G-250

8.0–28.0 ng

1–2.5 hr Nonhazardous staining in aqueous solution; premixed; mass spectrometry compatible

Coomassie Brilliant Blue R-250

36.0–47.0 ng

2.5 hr

Simple and consistent; mass spectrometry compatible

Silver Stains
Silver Stain Plus kit(Gottlieb and Chavko 1987)

0.6–1.2 ng

1.5 hr

Simple and robust; mass spectrometry compatible

Silver stain (Merril et al. 1981)

0.6–1.2 ng

2 hr

Stains glycoproteins, lipoproteins, lipopolysaccharides, nucleic acids

Fluorescent Stains
Oriole fluorescent gel stain

0.5–1.0 ng

1.5 hr	Rapid fluorescent gel stain; no destaining; mass spectrometry compatible;
compatible only with UV excitation

Flamingo fluorescent gel stain 0.25–0.5 ng
5 hr
				

High sensitivity; broad dynamic range; no destaining; simple;
mass spectrometry compatible; excellent for LED and laser-based scanners

Nucleic Acid Stains
Ethidium bromide

Classic fluorescent DNA stain

50.0 ng

1 hr

See Also

Coomassie Stains

Precastpolyacrylamide gels:pages 196, 202.

QC Colloidal Coomassie Stain
The QC colloidal Coomassie stain provides sensitivity
down to ~3 ng BSA, low background end-point staining,
and the level of reproducibility needed to meet regulatory
standards. Additionally, it is formulated to be ready to use
and environmentally friendly. Features include:
■

■

■

■

■

Imaging systems:
page 284.

QC Colloidal
Coomassie Stain

Low background, high sensitivity, superior reproducibility

Bio-Safe
Coomassie Stain

 nvironmentally friendly formulation — no addition ofE
methanol or acetic acid required; eliminates the needfor hazardous waste disposal
 lexible staining and destaining times — from 1 hrF
to overnight o alcohol addition or dilution steps necessary whenN
staining polyacrylamide gels
One-part, ready-to-use colloidal Coomassie stain

For More Information
Web: bio-rad.com/coomassie
Download bulletin: 6385

Bio-Safe Coomassie Stain
Bio-Safe Coomassie Brilliant Blue G-250 stain is apremixed, ready-to-use, nonhazardous solution that
does not require the use of methanol and acetic acid fordestaining. Bio-Safe Coomassie stain produces blue
bands on a clear background and is fast, simple, sensitive,
and convenient. Advantages include:■

Coomassie Brilliant Blue R-250 Staining
and Destaining Solutions
Coomassie Brilliant Blue R-250 staining solution is the fastestand easiest way to stain Criterion or other polyacrylamide
protein gels using Coomassie stain. Coomassie R-250
staining and destaining solutions are ready to use.

In the U.S., call toll free at 1-800-424-6723

 taining in aqueous solution — no special handlingSor fume hood requirements■

Visibility of bands while gel is in the stain■

No solvent waste problems or disposal costs

For More Information
Web: bio-rad.com/coomassie
Download bulletin: 2423

Electrophoresis and Blotting | 221

Protein Electrophoresis
Protein Stains

bio-rad.com/proteinstains

Ordering Information
Catalog #Description

Price, U.S. $QC Colloidal Coomassie Stain
1610803
QC Colloidal Coomassie Stain, 1 L

86.00

Bio-Safe Coomassie Stain
1610786
Bio-Safe Coomassie Stain, 1 L
1610787
Bio-Safe Coomassie Stain, 5 L

80.00
310.00

Coomassie Brilliant Blue R-250 Staining and Destaining Solutions
Coomassie Brilliant Blue R-250 Staining Solutions Kit, includes 1 L Coomassie Brilliant Blue R-250
1610435
staining solution, 2 x 1 L Coomassie Brilliant Blue R-250 destaining solution
1610436
Coomassie Brilliant Blue R-250 Staining Solution, 1 L
1610437
Coomassie Brilliant Blue R-250 Staining Solution, 4 x 1 L
1610438
Coomassie Brilliant Blue R-250 Destaining Solution, 1 L
1610439
Coomassie Brilliant Blue R-250 Destaining Solution, 4 x 1 L

52.00
215.0039.00
160.00

Coomassie Stain Powders
1610400
Coomassie Brilliant Blue R-250, 10 g
1610406
Coomassie Brilliant Blue G-250, 10 g

20.00
36.00

135.00Silver Stains
Silver Stain Plus Kit
Silver Stain Plus kit provides the most sensitive and easiest
to use silver stain. It is derived from the method developedby Gottlieb and Chavko (1987) for detection of native anddenatured eukaryotic DNA in agarose gels. The chemistry
has been modified so that it is ideal for both proteins andnucleic acids in polyacrylamide and agarose gels.
The Silver Stain Plus kit:■■

Detects nanogram quantities of protein and DNA
Eliminates background by preventing silver precipitationin the gel matrix■

Does not require destaining■

Stains 13 full-size gels or 40 mini gels

Dodeca Silver Stain Kits
Dodeca silver stain kits are designed especially for use
with Dodeca stainers. The kits are available in two sizes,
designed to fill the small or large Dodeca stainer tomaximum capacity. Both kits have been optimized to deliver
high-sensitivity, convenient, and reproducible staining oflarge numbers of gels. The kits are based on the method
described by Sinha et al. (2001), in which protein-boundsilver ions are chemically reduced to form visible metallic
silver. The Dodeca silver stain kits detect nanogram levels ofproteins in polyacrylamide gels in less than 3 hours.
Bio-Rad Silver Stain Kit
Bio-Rad’s original silver stain kit, derived from themethod of Merril et al. (1981), is ideal for staining
polysaccharides and highly glycosylated proteins thatare difficult to stain with the Silver Stain Plus kit. This kit
will stain 24 full-size gels or 48 mini gels in 2 hours.
For More Information
Web: bio-rad.com/silverstain
Download bulletin: 1089

Ordering Information
Catalog #Description

Price, U.S. $Silver Stain Plus Kit
1610449
Silver Stain Plus Kit, includes fixative enhancer concentrate, silver complex solution, reduction moderator
solution, image development reagent, development accelerator reagent, stains 13 full size or 40 mini gels
1610448Development Accelerator Reagent, 50 g
1610461*
Fixative Enhancer Concentrate, 1 L
1610462*
Silver Complex Solution, 100 ml
1610463*
Reduction Moderator Solution, 100 ml
1610464*
Image Development Reagent, 100 ml

225.00
25.00
61.00
29.00
44.00
56.00

* Hazardous shipping charges may apply.

222 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Protein Stains

bio-rad.com/proteinstains

Ordering Information
Catalog #Description

Price, U.S. $Silver Stain Plus Kit
1610480
Dodeca Silver Stain Kit for Protein Gels, large
1610481
Dodeca Silver Stain Kit for Protein Gels
1610472
Image Developer Concentrate
1610473
Sensitizer Concentrate, 700 ml
1610474
Sensitizer Concentrate, 1 L
1610475
Background Reducer Concentrate, 75 ml
1610476
Background Reducer Concentrate, 125 ml
1610477
Silver Reagent Concentrate, 140 ml
1610478
Silver Reagent Concentrate, 200 ml
1610479
Development Buffer Concentrate, 700 ml
1610482
Development Buffer Concentrate, 1 L

225.00
25.00
61.00
29.00

44.00
56.00

Bio-Rad Silver Stain Kit*
1610443
Silver Stain Kit, includes oxidizer concentrate, silver reagent concentrate, silver stain developer,
stains 20 full-size or 48 mini gels
1610444
Oxidizer Concentrate, 480 ml
1610445
Silver Reagent Concentrate, 480 ml

195.0066.00
66.00* Hazardous shipping charges may apply.

See Also

Fluorescent Stains

Imaging systems:
page 284.

Oriole Fluorescent Gel Stain
Oriole stain is a an easy-to-use, fast, and sensitive
fluorescent protein gel stain.
■

■

■

■

Precastpolyacrylamide gels:pages 196, 202.
2-D electrophoresis:
page 224.

 ne-step protocol, no fixing or destaining required,O
full sensitivity achieved in 90 min
 ull compatibility with downstream proteolysis and massF
spectrometric analysis
Nanogram sensitivity and low background ompatible with UV excitation imagers such as theC
GelDoc Go and ChemiDoc MP imaging systems

Flamingo Fluorescent Gel Stain
This easy-to-use, economical gel stain is for use with
the ChemiDoc MP system and a variety of fluorescenceimaging systems.
■

Two-step protocol that can be completed in as little as 5 hr■Compatible with mass spectrometry

2-D gel stained with Oriole stain.
E. coli protein (40 µg) was run on
an 11 cm pH 5–8 ReadyStrip IPG
strip for the first dimension and a
Tris-HCl 8–16% Criterion gel forthe second dimension.

■■

Flamingo fluorescent gel stain.
E. coli protein sample (10 µg).

 ide dynamic range and highly linear responseW(3 orders of magnitude)
Less than 1 ng protein can be detected

For More Information
Web: bio-rad.com/fluorescentstain
Download bulletins: Flamingo — 5346, 5705, 5754;
Oriole — 5900, 5921, 5991

Ordering Information
Catalog #Description

Price, U.S. $Oriole Fluorescent Gel Stain
1610495
Oriole Fluorescent Gel Stain, 1x solution, 200 ml
1610496
Oriole Fluorescent Gel Stain, 1x solution, 1 L
1610497
Oriole Fluorescent Gel Stain, 1x solution, 5 L

82.00
185.00845.00

Flamingo Fluorescent Gel Stain
1610490
Flamingo Fluorescent Gel Stain, 10x solution, 20 ml
1610491
Flamingo Fluorescent Gel Stain, 10x solution, 100 ml
1610492
Flamingo Fluorescent Gel Stain, 10x solution, 500 ml

67.00
195.00930.00In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 223

Protein Electrophoresis
2-D Electrophoresis

bio-rad.com/2Dsystems

2-D Electrophoresis
IEF is primarily used as the first dimension of separation in 2-D analysis; 2-D electrophoresis is used to separate
complex protein samples based on pI and MW. IEF separations can be performed using two techniques: either with an
IPG strip with ampholytes covalently bound to the gel or with carrier ampholytes that move through the gel to generatea pH gradient. Bio-Rad offers products for both techniques. Bio-Rad’s first-dimension products are compatible withsecond-dimension SDS-PAGE systems in mini, midi, and large formats. For more information, see page 181.
For preparative IEF products see page 229.
Learn More about the Technology
Web: bio-rad.com/tech/2delectro

 ROTEAN i12 IEF System Accessories
P
Accessories for the discontinued PROTEAN i12 system
can also be purchased individually. Six sizes of focusing
and rehydration/equilibration trays are available as well
as replacement electrodes, cleaning supplies, and othersystem-related items.
Cup loading is an option for improving your 2-D results,
especially for proteins with extreme pIs. The PROTEAN i12
sample cup holder can be used with all of the PROTEAN
i12 focusing trays. It effortlessly clips onto the tray andforms a secure seal that prevents leaking but won’t
damage the IPG strip. The disposable sample cups
prevent sample contamination.

For More Information
Web: bio-rad.com/2dworkflow

Focusing Trays

Rehydration Trays

i12 Sample Cup Holder

For More Information
Web: bio-rad.com/proteani12
Download bulletins: 2651, 6097, 6138, 6139, and 6140

Ordering Information
Catalog #Description

PROTEAN i12 IEF System Accessories
1646107i12 7 cm Focusing Tray, includes 2 IPG strip retainers
1646111i12 11 cm Focusing Tray, includes 2 IPG strip retainers
1646113i12 13 cm Focusing Tray, includes 2 IPG strip retainers
1646117i12 17 cm Focusing Tray, includes 2 IPG strip retainers
1646118i12 18 cm Focusing Tray, includes 2 IPG strip retainers
1646124i12 24 cm Focusing Tray, includes 2 IPG strip retainers
1654035
i12 7 cm Rehydration/Equilibration Tray, includes lids, 25
1654025
i12 11 cm Rehydration/Equilibration Tray, includes lids, 25
1646313i12 13 cm Rehydration/Equilibration Tray, includes lids, 25
1654015i12 17 cm Rehydration/Equilibration Tray, includes lids, 25
1654041i12 18 cm Rehydration/Equilibration Tray, includes lids, 25
1654043i12 24 cm Rehydration/Equilibration Tray, includes lids, 25
1646040
IPG Strip Retainers, 21646020i12 Sample Cup Holder, includes 25 sample cups
1646021i12 Sample Cups, 25
1646030
Gel-Side Up Electrode Wicks, 100
1646031
Gel-Side Down Electrode Wicks, 500
1646012
Negative Electrode Assembly
1646011
Positive Electrode Assembly
1646010
Electrode Assembly Pair, includes 1 positive and 1 negative electrode assembly
1654072
Cleaning Brushes, 21610722
Cleaning Concentrate, 1 kg, 50x
1646060
USB Flash Drive, 21646050
Stylus, 31632129
Mineral Oil, 500 ml
1654070
Forceps, 11654071
Electrode Wicks, precut, 500

224 | Electrophoresis and Blotting

Price, U.S. $
80.00
80.00
80.00
80.00
80.00
80.00
120.00120.00200.00150.00240.00160.00
18.00
36.00
26.00
31.00
31.00
610.00610.001,100.00
24.00
59.00
54.00
60.00
27.00
50.00
12.00

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
2-D Electrophoresis

bio-rad.com/2Dsystems

See Also

ReadyStrip IPG Strips

ReadyPrep 2-Dstarter kit: page 226.

ReadyStrip IPG strips are available in five different strip
lengths and in a wide selection of pH gradients, with
3 or 7 pH units per strip. Shorter strips are useful formethod development while longer strips provide thebest separation possible with higher protein loads.
ReadyStrip IPG strips are thoroughly tested for quality andperformance to deliver 2-D gel-to-gel reproducibility.

ReadyStrip IPG strips
are preprinted toindicate anode end(+) and pH range; in
addition, a barcode
is printed on the
24 cm strip.

Relative Focusing PowerThe 7 cm pH 3–10 strip is arbitrarily assigned a baseline
focusing power of 1.0 in order to calculate the relative
focusing power of the other strips.

Design Features
Stringent gel length tolerances of ±2 mm■■

Anode and pH range clearly printed on each strip, with■barcoding on 24 cm strips■

calculate the ratio of the strip lengths. Compared to a
7 cm strip, an 11 cm strip has a relative focusing powerof 11/7 cm = 1.6

Consistent backing lengths for self-centering on 2-D gels

Gradient Selection
Standard broad range pH gradients for maximum 		
separation on a single gel■■■Narrow range gradients for greater resolution (morecm of gel per pH unit)■

Strips with the same pH range but different lengths — omprehensive offering that increases resolving powerCin the first dimension with overlapping pH ranges

Strips with the same length but different pH range —calculate the ratio of the pH ranges. Compared to a
pH 3–10 strip (7 pH units), a pH 5–8 strip (3 pH units)
has a relative focusing power of 7/3 = 2.3

For More Information
Web: bio-rad.com/readystripIPG
Download bulletin: 2442

Relative Focusing Power of IPG Strips
pH
Strip Range*

3

4 5

6

7

Relative Focusing Power
8

9 10

7 cm

11 cm

17 cm

18 cm

ReadyStrip IEF Buffer
24 cm

3–10

•
•

7–10

Broad Range
3–10									

1x

1.6x

2.4x

2.6x

3.4x

3–10 nonlinear (NL)									

1x

1.6x

2.4x

2.6x

3.4x

3–6									

2.3x

3.7x

5.7x

6.0x

8.0x

5–8									

2.3x

3.7x

5.7x

6.0x

8.0x

7–10									

2.3x

3.7x

5.7x

6.0x

8.0x		

4–7									

2.3x

3.7x

5.7x

6.0x

8.0x

Narrow Range

•
•
•

•

* Strips are designed with sufficient overlap to allow spot matching while limiting the extent of redundant data.

See Also

Ordering Information
pH Range

7 cm

11 cm

17 cm

18 cm

24 cm

ReadyStrip IPG Strips, 12 per Package
pH 3–101632000
1632014
pH 3–10 NL*
16320021632016
pH 3–616320031632017
pH 4–71632001
1632015
pH 5–81632004
1632018
pH 7–101632005
163201916320071632009163201016320081632011163201216320321632033
1632035
1632034
1632036163203716320421632043
1632045
163204416320461632047

Bio-Lyte ampholytes:
page 220.

ReadyStrip IEF Buffers** and Accessories
1632094
Bio-Lyte 3/10 Ampholyte, 100x, 1 ml
1632093
ReadyStrip 100x 7–10 Buffer, includes only ampholytes, 1 ml
1632099
ReadyStrip Instruction Manual, free upon request with ReadyStrip purchases* NL, nonlinear gradient.
** Dilute ReadyStrip buffers to 1x in each sample to obtain a final concentration of 0.2% ampholyte.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 225

Protein Electrophoresis
2-D Electrophoresis

bio-rad.com/2Dsystems

ReadyPrep Reagents for First-Dimension IEF
ReadyPrep reagents ensure success with first- and second-dimension separations. The 2-D starter kit is the ideal tool forlearning to use the PROTEAN i12 IEF system and for perfecting technique. Premixed buffers and individual reagents areavailable for each step of the 2-D process. For preparation of protein samples for 2-D electrophoresis, see page 2.

See Also
ReadyStrip IPG strips
and IEF buffers:
page 225.
Criterion system:
page 201.

ReadyPrep 2-D Starter Kit
The ReadyPrep 2-D starter kit is intended for first-time usersof the discontinued PROTEAN i12 IEF cell and ReadyStrip
IPG strips. The kit contains tested premixed reagentsrequired for first and second dimension separations, areference manual with technical tips, and a known protein
sample. The ReadyPrep 2-D starter kit includes all reagentsneeded to:
■

■

■

■

Prepare an E. coli protein sample
Rehydrate IPG strips with sample
Equilibrate IPG strips for SDS-PAGE
Overlay IPG strips with agarose on SDS-PAGE gels

This kit contains enough material to complete either six 17 cm
IPG strips, ten 11 cm strips, or sixteen 7 cm IPG strips.

For More Information
Web: bio-rad.com/readyprep2d

2-D Starter Kit Contents
Vials/Kit

Vials/Kit

E. coli protein sample, 2.7 mg

1

30% glycerol solution, 70 ml

1

ReadyPrep 2-D starter kit rehydration/sample buffer, 10 ml

1

ReadyPrep overlay agarose, 50 ml

1

ReadyPrep equilibration buffer I, 20 ml

2

Iodoacetamide, 0.5 g

2

ReadyPrep equilibration buffer II, 20 ml

2

Nanopure water, 15 ml

1

Ordering Information
Catalog #Description

1632105

 eadyPrep 2-D Starter Kit, includes E. coli protein sample and reagents sufficient to rehydrate, focus, andRtransfer to second-dimension gels; ReadyStrip IPG strips, precast SDS-PAGE gels, and gel stains not included
E. coli Protein Sample, lyophilized, 2.7 mg

1632110226 | Electrophoresis and Blotting

Price, U.S. $260.0057.00

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
2-D Electrophoresis

bio-rad.com/2Dsystems

See Also

2-D Premixed Buffers and Individual Reagents
Streamline 2-D experiments and reduce variables with
Bio-Rad’s convenient premixed buffers and protein sample,
tested for consistent 2-D performance with IPG strips:■

ReadyPrep 2-D starter kit rehydration/sample buffer— a standard formulation appropriate for many protein
samples■ReadyPrep 2-D starter kit equilibration buffer I —premixed with DTT for the first equilibration step in the
DTT/iodoacetamide alkylation method■

ReadyPrep 2-D starter kit equilibration buffer II —add iodoacetamide and use for the second equilibration
step in the DTT/iodoacetamide alkylation method; thisbuffer can also be used for single-step alkylation byadding TBP and acrylamide■E. coli protein sample — this complex protein sample
is performance tested to give a consistent pattern whenused with ReadyPrep 2-D starter kit rehydration/sample
buffer; use this sample as a control to validate your2-D system and protocol before running more difficult
experimental samples

Reducing and Alkylating Agents
Either DTT or TBP can be used for IEF and during
equilibration prior to SDS-PAGE. Alkylation withiodoacetamide is the standard method to prevent
reoxidation during second-dimension SDS-PAGE.
Reduction and alkylation can also occur at the sample
preparation stage. See the ReadyPrep reduction-alkylation
kit, page 4.

Protein sample
preparation products:
page 2.
ReadyPrep reductionalkylation kit:
page 4.

Overlay Agaroses
Bromophenol blue tracking dye is incorporated into thissolution to allow monitoring of electrophoresis runs. Use
ReadyPrep overlay agarose, a low melting point agarose,
to secure IPG strips in place for most applications. For
second-dimension runs in the PROTEAN Plus cell, in
which the IPG strip is oriented perpendicular to thelaboratory bench, firmer PROTEAN Plus overlay agarose isrecommended to secure the IPG strip.
Individual Reagents and Detergent
Urea and Tris as well as CHAPS detergent are available.
For More Information
Web: bio-rad.com/2dreagents

Ordering Information
Catalog #Description

1632106

 eadyPrep 2-D Starter Kit Rehydration/Sample Buffer, 10 ml, 8 M urea, 2% CHAPS, 50 mM DTT,
R
0.2% Bio-Lyte 3/10 ampholyte, 0.001% bromophenol blue
ReadyPrep 2-D Starter Kit Equilibration Buffer I, with DTT, 10 ml, 375 mM Tris-HCl, pH 8.8, 6 M urea,
2% SDS, 2% DTT
ReadyPrep 2-D Starter Kit Equilibration Buffer II, without DTT or iodoacetamide, 20 ml, 375 mM Tris-HCl,
pH 8.8, 6 M urea, 2% SDS
ReadyPrep Proteomics Grade Water, 500 ml
Dithiothreitol (DTT), 1 g
Dithiothreitol (DTT), 5 g
Tributylphosphine (TBP), 200 mM, 0.6 ml
Iodoacetamide, 30 g
Urea, 1 kg
Tris, 1 kg

1632107
16321081632091
161061016106111632101
163210916107311610719

Control Sample
1632110
E. coli Protein Sample, lyophilized, 2.7 mg
Overlay Agaroses
1632111
ReadyPrep Proteomics Grade Overlay Agarose, 50 ml, 0.5% low melting point agarose in
1x Tris/glycine/SDS and 0.003% bromophenol blue
1632092
PROTEAN Plus Proteomics Grade Overlay Agarose, 125 ml, 0.75% agarose in 1x Tris/glycine/SDS
and 0.003% bromophenol blue

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
44.00
44.00
37.00
40.00
27.00
81.00
44.00
145.00
76.00
84.00
57.00
21.00
71.00

Electrophoresis and Blotting | 227

Protein Electrophoresis
2-D Electrophoresis

bio-rad.com/2Dsystems

Mini-Format Analytical IEF
See Also
PowerPac HV andPowerPac Universal
power supplies:
page 185.
IEF standards:
page 191.
Bio-Lyte ampholytes:
page 220.
Acrylamide
gel-casting reagents:
page 216.
Agarose:page 218.

Model 111 Mini IEF Cell
This compact cell performs analytical IEF, including
isoenzyme separation, forensic applications, and clinicaldeterminations. Use it to screen large numbers of samples
or for quickly running a few samples to determine thepI of a protein of interest. It requires only a 500 V power
supply. Please note that this unit does not use IPG strips.
Features include:■Unique inverted format — condensation cannot

disrupt electrophoresis results■

No external cooling required■

Bufferless operation — no wicks required■

Casting tray suitable for both agarose andpolyacrylamide gels■

Easy-to-clean, removable graphite electrodes■

Small footprint of 21 x 11.5 x 4.2 cm

For More Information
Web: bio-rad.com/model111
Download bulletin: M1702975

Ordering Information
Catalog #Description

1702975 odel 111 Mini IEF Cell, includes chamber and lid, graphite electrodes, casting tray, 5 glass plates,M
50 sheets of gel support film for polyacrylamide, 5 sample templates

Accessories
1702980
17029811702982
1702983
17029841702985

Graphite Electrodes, 2Mini Casting Tray
Glass Plates, 12.5 cm x 6.5 cm x 1.5 mm, 5
Gel Support Film for Polyacrylamide, 12.5 x 6.5 cm, 50 sheets
Gel Support Film for Agarose, 12.5 x 6.5 cm, 50 sheets
Sample Templates, 5

228 | Electrophoresis and Blotting

Price, U.S. $1,245.00

83.00
295.0084.00
220.00185.0044.00

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Preparative Electrophoresis

bio-rad.com/prepelectro

Preparative Electrophoresis
Preparative electrophoresis devices fractionate and purify nanogram to gram quantities of proteins or nucleic acids via
liquid phase IEF electrophoresis or continuous electroelution from gels. These devices separate and purify molecules
according to their molecular mass (using SDS-PAGE or agarose gel electrophoresis), pI (using liquid-phase IEF), or a
combination of both molecular mass and pI (using native PAGE or preparative 2-D electrophoresis). These devices include:■■

See Also
Protein sample
preparation products:
page 2.

 odel 491 prep cell and mini prep cell — performMhigh-resolution separations of proteins and nucleic acids
by continuous-elution gel electrophoresis (PAGE) odel 422 electro-eluter — elutes macromoleculesMfrom gel slices

Preparative Electrophoresis Product Selection Guide
Product

Molecules Purified

Run Time

Bulletin

Model 491 prep cell229
SDS-PAGE or native PAGE
				
				

Protein (1–500 mg)
DNA (50–300 µg)
RNA (≤1 mg)

4–10 hr

1964

Mini prep cell229
SDS-PAGE or native PAGE
				
				

Protein (0.5–1,000 µg)
DNA (≤10 µg)
RNA (≤20 µg)

4–10 hr

1964

Model 422 electro-eluter
231			Protein (gel load limits)
DNA (≤3 mm thick gels)

3–5 hr—Page

Method of Purification

Electroelution from excised
gel pieces

Preparative PAGE Cells
See Also

Model 491 Prep Cell and Mini Prep Cell
The Model 491 prep cell and mini prep cells separate
biomolecules (protein or nucleic acids) by continuouselution electrophoresis. Samples are electrophoresed
through a cylindrical gel matrix, where they are separated
into ring-shaped bands. As individual bands migrate off
the bottom of the gel, they are collected in discrete liquid
fractions. The Model 491 prep cell and the mini prep cellallow resolution of proteins differing in MW by as little as
2%. With these prep cell systems, you can:
■

■

■

■

Purify nanogram to milligram quantities of target protein
 eparate proteins that differ in MW by as little as 1 kDa
S
by using SDS-PAGE eparate proteins by mass and charge with pI differencesS
as little as 0.1 pH units by using native PAGE
 eparate large proteins or DNA fragments (up to 18 kb)
S
by using agarose gel electrophoresis

For More Information
Web: bio-rad.com/prepPAGE
Download bulletins: 1964, 3153, and 3161

In the U.S., call toll free at 1-800-424-6723

PowerPac Universal
and PowerPac HV
power supplies:
page 185.

Upper­electrophoresis
bufferElution buffer

Cooling core

To peristaltic pumpand fraction collector
Lower ­electrophoresis
bufferElution­collection
tube
Gel tube
Protein bands

Patented­ lutionechamber
Buffer ­recirculation pumpContinuous-elution electrophoresis in the Model 491 prep cell.
Samples are electrophoresed through the cylindrical gel matrix where
they are separated into ring-shaped bands. Individual bands migrate
off the bottom of the gel and are collected in discrete liquid fractions
available for assay and characterization.

Electrophoresis and Blotting | 229

Protein Electrophoresis
Preparative Electrophoresis

bio-rad.com/prepelectro

Specifications
Model 491 Prep Cell

Mini Prep Cell

Sample capacity (mass/volume)

1–500 mg/0.5–15 ml

0.5–1.0 mg/50–500 µl

Gel tube dimensions

28 and 37 mm ID, 14 cm length

7 mm ID, 13 cm length

CoolingGlazed alumina-ceramic tube

Not necessary

Electrical limits

500 V, 40 mA, 20 W
(PowerPac HV or PowerPac Universal recommended)

500 V, 10 mA, 5 W(PowerPac HV or PowerPac Universal recommended)

Elution buffer flow rate

1 ml/min

0.1 ml/min

Auxiliary equipment required

Fraction collector, power supply, peristaltic pump

Fraction collector, power supply

Ordering Information
Catalog #Description

Price, U.S. $Model 491 Cells
1702928
Model 491 Prep Cell without Buffer Recirculation Pump

95.00

Replacement Parts and Accessories for the Model 491 Prep Cell
1702944
Prep Cell Casting Stand
1702932
Small Gel Tube Assembly, 28 mm ID
1702933
Large Gel Tube Assembly, 37 mm ID
1702934
Cooling Finger Assembly, includes feedline connectors
1702937
Dialysis Membranes, precut, 51702939
Sample Application/Overlay Buffer Kit, includes sample loading guide, syringe with tubing

265.00465.00480.00840.0059.00
86.00

Replacement Parts and Accessories for the Mini Prep Cell
1702909
Gel Tubes, 21702913
Sample Application/Purge Kit
1702947
Peristaltic Pump Adaptor Kit, for 0.8 mm tubing1702948
Elution Frit Kit, with 5 dialysis membranes, MW cutoff 3,500
1702911
Elution Frit Kit, with 5 dialysis membranes, MW cutoff 6,000

95.00
66.00
24.00
64.00
66.00

230 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Protein Electrophoresis
Preparative Electrophoresis

bio-rad.com/prepelectro

Preparative Electroelution Cells
Model 422 Electro-Eluter
The Model 422 electro-eluter is an electroelution cell for
preparative recovery of biomolecules from agarose andacrylamide gels. Easy to assemble, the electro-eluter has
six vertical glass tubes connecting the upper and lower
buffer chambers. A frit at the bottom of each tube retains
the gel slice but permits macromolecules to migrate throughwhen current is applied. When the macromolecules havepassed through the frit, they are collected (in the membranecap) for further analysis or testing.
Depending on the buffer system, the Model 422 electroeluter can be used for protein elution or dialysis. In allcases, setup is quick and easy and the sample is collected
in 400–600 µl. The Model 422 electro-eluter can be used
for one to six samples without increasing the run time
(3–5 hours) or decreasing sample yield.
For More Information
Web: bio-rad.com/electroelution

Specifications
Elution capacity
Collection volume
Buffer volume
Glass tube dimensions
Recommended power supply
Dimensions (W x D x H)
Weight

1–6 samples
400–600 µl
700 ml1 cm (ID) x 6 cm (long)
PowerPac Universal
12 x 16 x 18 cm
1.1 kg (2.4 lb)

Ordering Information
Catalog #Description

Accessories
1652976
16529851652986
165298716529781651988

Model 422 Electro-Eluter
Membrane Caps, clear, MW cutoff 12,000–15,000, 12
Membrane Caps, green, MW cutoff 3,500, 12
Frits, 12
Glass Tubes, 6Grommets and Stoppers, 8

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
67.00
67.00
67.00
46.00
73.00
98.00

Electrophoresis and Blotting | 231

Western Blotting
Stain-Free Western Blotting Protocol

VideosCustomer
Testimonial

bio-rad.com/blotting

Western Blotting
Bio-Rad’s western blotting products include the Stain-Free western blotting workflow, systems for protein transfers, blotting
membranes, filter paper, premixed blotting buffers, reagents, protein standards, and detection kits.
Learn More about the Technology
Web: bio-rad.com/learnwestern

bio-rad.com/WByale

Product
Demonstration

bio-rad.com/
WBstainfree

Stain-Free Western Blotting Protocol
Bio-Rad’s Stain-Free western blotting workflow — consisting of TGX Stain-Free precast gels, the Trans-Blot Turbo system,and the ChemiDoc MP or ChemiDoc imaging system — incorporates traditional blotting techniques with innovativetechnology. The five-step streamlined protocol allows quick confirmation of gels and blot transfer quality prior to western
blotting and provides total protein blot normalization for rapid and robust quantitation.
Five Steps
1. S
 eparate proteins — Mini-PROTEAN TGX Stain-Free precast gels and Criterion TGX Stain-Free precast gels offer fast,
superior protein separation. TGX Stain-Free precast gels feature proprietary in-gel chemistry, enabling high-quality protein
separation in as little as 15 minutes (pages 196, 202–207).
2. V isualize proteins — protein separation is visualized and confirmed, using Stain-Free technology, after 1 minuteactivation on the ChemiDoc MP or ChemiDoc imaging system. Stain-Free technology is a sensitive, time-saving
alternative to traditional Coomassie staining (page 285).
3. T ransfer proteins — Trans-Blot Turbo system, a rapid protein transfer apparatus, reduces transfer protocols to as little
as 3 minutes across a broad MW range (page 235).
4. A
 ssess protein transfer — ChemiDoc MP or ChemiDoc imaging system paired with Stain-Free technology enables
instant verification of protein transfer (page 285).
5. N ormalize and quantitate — ChemiDoc MP or ChemiDoc imaging system and Image Lab desktop software validate
western blotting data via total protein normalization as an alternative to using housekeeping proteins. By normalizing tototal protein, stripping and reprobing is no longer necessary.

1
Separate Proteins
Rapidly separate
proteins with TGX
Stain-Free precast gels

232 | Electrophoresis and Blotting

2

3

4

Visualize Separation
Immediately visualize
separation using stainfree technology and theChemiDoc MP or ChemiDoc
imaging system

Transfer Proteins
Use the Trans-Blot
Turbo system for rapid
and efficient proteintransfer

Assess Transfer
Verify high-qualitytransfer by instantly
imaging proteins on themembrane

5
Normalize and Quantitate
Perform multiplex imaging
and validate results bytotal protein normalizationon the ChemiDoc MP or
ChemiDoc imaging system

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Stain-Free Western Blotting Protocol

bio-rad.com/blotting

Bio-Rad Stain-Free Western Blotting vs. Traditional Western Workflow
Time

Bio-Rad Stain-Free Western Blotting Workflow

1

Data

Electrophoresis			TGX Stain-Free gel			
Criterion cell+

Pre-transfer gel
Stain-Free image tocheck sample integrity
and separation quality

20–30 minChemiDoc MP/
ChemiDoc system

2

Post-transfer gel
Stain-Free image
to measure transfer
efficiency

Transfer			Trans-Blot Turbo system
			ChemiDoc MP/
ChemiDoc system+

3–10 minTotal time:
~5 hr

3

Antibody Incubation			
EveryBlot blocking buffer			Clarity western

Stain-Free blot image
as loading control~4 hr

ECL substrate

4

Imaging and Analysis					orChemiDoc MP/
10–15 min			ChemiDoc system(no need to strip
and reprobe)

Target proteins(chemiluminescence)

Traditional Western Workflow		Time

Target proteins(fluorescence)

Data

		

1

Gel Preparation		

>1 hr gel prep—

2

Electrophoresis		

~1 hr gel run—

3

Transfer		

1–3 hr—4

Antibody Incubation

~5 hr

—

5

Imaging and Analysis>30 min		

Target proteins

6

Strip and Reprobe		~5 hr		

Loading control		Total time:
16 hr(Often need reprobing
for actin/tubulin asloading control)

For More Information
Web: bio-rad.com/stainfreewestern

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 233

Western Blotting
Transfer Devices

bio-rad.com/blotting

Transfer Devices
Overview of Blot Transfer Systems
Rapid transfer systems — The Trans-Blot Turbo system (page 235) uses prewetted, prepackaged consumables for
rapid transfer of proteins in as little as 3 minutes across a wide range of protein molecular weights■

 emi-dry transfer systems — Trans-Blot SD for rapid, high-intensity transfers, best suited for mid-range proteins,S
10–100 kD or >200 kD (page 236)■

 ank transfer systems — ideal for most routine protein work, tank transfer systems provide efficient and quantitative proteinTtransfers over a broad MW range and are available with either plate or wire electrodes (page 237)■

 icrofiltration (dot blotting) and screening systems — used to determine working conditions for a new blotting assay orMin situations where protein separation is not required; suitable for both protein and nucleic acid blotting (page 240)■

Blotting Selection Guide
		Blotting area

Mini Trans-Blot

Criterion Blotter

Trans-Blot

Trans-Blot Plus

Trans-Blot SD

Trans-Blot Turbo

10.0 x 7.5 cm

15.0 x 9.4 cm

16.0 x 20.0 cm

28.0 x 26.5 cm

24.0 x 16.0 cm

15.0 x 11.0 cm

Gel capacity
2 Mini-PROTEAN
4 Mini-PROTEAN
3 PROTEAN II xi,
Three 26.5 x 28 cm 2 PROTEAN II gel		gels
or 2 Criterion gels
6 Criterion, orgels or 12 Criterion sandwiches,				
12 Mini-PROTEAN gelsstacked and				gels		
separated by						
dialysis membrane;						4 Mini-PROTEAN						
gels side by side;						
3 Criterion gels						
side by side
Number of gel
holders

2

2

3

3

Buffer requirement

1.2 L

1.3 L

3–4 L

10–12 L—
200 mlElectrode distance 4.0 cm
4.3 cm2 positions:
3 positions: 4.0,
Determined by				
4.0 and 8.0 cm
7.0, and 10.0 cm
thickness of the						
gel and membrane						sandwich and						
filter paper stack
Electrode dimensions—

Electrode materials Platinum wire
			
			
			
			

—
Platinum-coatedtitanium anodewith stainless-steel
cathode plates or
platinum wire—
Platinum-coatedtitanium anodewith stainless-steel
cathode plates or
platinum wire—
Platinum-coatedtitanium anode andstainless-steel
cathode plates

Transfer time					Wire electrodes
Standard: 16 hr
Standard: 60 min
Standard: 5 hr
High-intensity:
to overnight
Overnight: 16 hr
				
1 hr		
High-intensity:				
30 min–4 hr
Plate electrodes		Standard: 30 min			to overnight
				
				
Cooling
Blue cooling unit						Standard: 1–5 hr
Overnight: 16 hr
High-intensity:
30 min–1 hr

Sealed ice block or Super cooling coil
optional Criterion
blotter cooling unit		

2 midi gels
(13.5 x 8.5 cm),
4 mini gels
(7.0 x 8.5 cm)
or similar—Standard: 16 hr
High-intensity:
15 min–1 hr
Super cooling coil—
N/A
~8 mm dependingon gel thickness

25.0 x 18.0 cm

16.0 x 12.0 cm

Platinum-coatedtitanium anode andstainless-steel
cathode plates

Platinum-coatedtitanium anodeand stainless-steel
cathode plates——~30 min

3–10 min——Overall dimensions 12.0 x 16.0 x 18.0 cm 21.8 x 11.8 x 15.0 cm 18.0 x 9.5 x 24.0 cm 30.0 x 17.3 x 39.4 cm 37.0 x 24.0 x 11.0 cm 26.0 x 21.0 x 20.0 cm(W x L x H)				

234 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Transfer Devices

bio-rad.com/blotting

Semi-Dry and Rapid Blotting Systems
Video

Trans-Blot Turbo Transfer System

Product
Demonstration

The Trans-Blot Turbo transfer system represents thenext generation of protein transfer. The Trans-Blot Turbo
integrates speed, reproducible performance, and ease ofuse into a complete system, providing results faster thanany other method currently available.
The Trans-Blot Turbo blotting system combines traditionalblotting techniques with modern filter paper and buffers,
allowing rapid transfer of proteins with minimal preparation
time. By providing the entire system in a ready-to-use
format, researchers can obtain their results faster andeasier with reproducibility that is difficult to achieve bytraditional tank and semi-dry blotting methods.
Rapid, High-Throughput Transfer
Transfers standard mini or midi gels in as little as 3 minbio-rad.com/TBTknow

Ready-to-Assemble (RTA) Transfer Kits
Kits provide enough consumables for 40 blots
■

■

■

■

Efficient transfer of high and low MW proteins■

Can transfer 1–4 mini or 1–2 midi gels in a single run■

No cooling period required between runs■■

 pecialized protocol for Mini-PROTEAN TGX gel transfer
S
in 3 minNo need to pre-equilibrate gels prior to transfer

Ready-to-Use Transfer Packs
Ready-to-use transfer packs eliminate the need for buffer
and membrane preparation■■

Transfer packs available with nitrocellulose and PVDF■

Proprietary buffer included in each transfer pack■

 onsists of pre-cut membranes, pre-cut filter pads, andC
specially formulated transfer buffer its available with nitrocellulose, PVDF, and lowK
fluorescence PVDF

Flexible Design
Option to either use rapid preset protocols or customize
transfer conditions■■

Accommodates traditional semi-dry consumables■

Compatible with various gel types and percentages■

Ability to customize and store protocols within the instrument■Integrated power supply means no external power supply
is needed

Environmentally Friendly
Environmentally safe consumables eliminate disposal cost■■

Single-use consumables reduce waste

For More Information
Web: bio-rad.com/turbo
Request or download bulletin: 6039

Ordering Information
Catalog #Description

17001917
17001919
17001918
17001915
1704151
1704152

Trans-Blot Turbo Transfer Starter System, mini, PVDF
Trans-Blot Turbo Transfer Starter System, midi, PVDF
Trans-Blot Turbo Transfer Starter System, mini, nitrocellulose
Trans-Blot Turbo Transfer Starter System, midi, nitrocellulose
Trans-Blot Turbo Cassette, 1 cassetteTrans-Blot Turbo Base, base instrument, no cassettes included

Description
Trans-Blot Turbo Transfer Pack, PVDF, pkg of 10
Trans-Blot Turbo Transfer Pack, Nitrocellulose, pkg of 10
Trans-Blot Turbo RTA Transfer Kit, Nitrocellulose, for 40 blots
Trans-Blot Turbo RTA Transfer Kit, PVDF, for 40 blots
Trans-Blot Turbo RTA Transfer Kit, LF PVDF, for 40 blots

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $3,005.00

750.001,675.00

Mini(7 x 8.5 cm)

Price, U.S. $Midi(8.5 x 13.5 cm)

1704156
1704158
1704270
1704272
1704274

175.00155.00285.00305.00305.001704157
1704159
17042711704273
1704275

Price, U.S. $210.00185.00370.00415.00415.00Electrophoresis and Blotting | 235

Western Blotting
Transfer Devices

See Also
PowerPac HC power
supply: page 185.
Blotting buffers:
page 245.

bio-rad.com/blotting

Trans-Blot SD Semi-Dry Transfer Cell
The Trans-Blot SD semi-dry transfer cell allows fast andefficient blotting without buffer tank or gel cassettes.
Features include:■

Transfers in as little as 15–60 min■

Minimal buffer requirements■

Capacity to transfer multiple gel sizes■

Single-step locking system for simple setup■■

One-step locking system

Spring-mounted
electrodes latinum-coated titanium anode and stainless-steelPcathode plate electrodes that provide consistent andreliable transfers, durability, and years of use

DNA/RNA agarose
support frame

 afety cover to break the electrical current when lifted,
S
preventing electrical shock

In addition to western blotting, the Trans-Blot SD cell
can also transfer DNA and RNA using the unique
agarose gel semi-dry blotting support frame. The frame
protects fragile agarose gels from compression by theelectrodes. Southern and northern blot transfers can be
run in 10–35 minutes.
For More Information
Web: bio-rad.com/transblotsd
Download bulletin: 2895

1

Expanded view of the Trans-Blot SD
cell assembly:
1. Safety lid.
2. Cathode assembly with latches.
3. Filter paper.
4. Gel.
5. Membrane.
6. Filter paper.
7. Spring-loaded anode platform
mounted on four guideposts.
8. Power cables.
9. Base.

2
3
4
5
6
7
8

9

Specifications
Maximum gel size (W x L)

24 x 16 cm

Buffer requirement

200 mlGel capacity

4 Mini-PROTEAN precast gels, 4 Mini-PROTEAN handcast gels,
3 Criterion gels, or 1–3 PROTEAN II gel sandwiches*

Recommended power supply

PowerPac HC

Dimensions (W x L x H)

37 x 24 x 11 cm

Weight3.6 kg (7.9 lb)* Dialysis membrane between each gel sandwich.

Ordering Information
Catalog #Description

1703940*

 rans-Blot SD Semi-Dry Electrophoretic Transfer Cell, includes transfer cell, agarose gelTsupport frame, extra thick blot paper (7 x 8.4 cm, 60 sheets; 8 x 13.5 cm, 60 sheets; 14 x 16 cm,
30 sheets; 18 x 18.5 cm, 30 sheets)
Trans-Blot SD Cell and PowerPac HC Power Supply, 100–120/220–240 V,
includes #1703940 and #1645052

1703848
Accessories
1703947
1703942

Price, U.S. $Cathode Plate, stainless-steel upper electrode
Anode Plate, platinum-coated lower electrode1,430.00

1,930.00

105.00625.00* The Trans-Blot SD semi-dry transfer cell requires the use of a microprocessor-controlled power supply.

236 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Transfer Devices

bio-rad.com/tankblotters

Wet/Tank Blotting Systems
See Also

Mini Trans-Blot Cell
This cell provides high-quality blotting of mini gels.
A component of the Mini-PROTEAN Tetra system,
the Mini Trans-Blot cell accommodates two gel holder
cassettes for electrophoretic transfer of mini-format gels.
■

■

■

■

■

Mini Trans-Blot cell components:
1. Buffer tank and lid.
2. Blue cooling unit.
3.	Foam pads.
4. Gel holder cassette.
5. Electrophoresis blotting module.

PowerPac Basic and
HC power supplies:
page 185.
Blotting membranes:
page 242.

1

Blot detection
reagents: page 247.

 bility to transfer two 10 x 7.5 cm gels in just 1 hr;A
low-intensity overnight transfers are also possible
 lacement of wire electrodes 4 cm apart for strongPelectrical fields and efficient protein transfer

Blotting buffers:
page 245.
5

 olor-coded cassettes and electrodes to ensure properCorientation of the gel during transfer
 lue cooling unit, contained within the Mini Trans-Blot cell,Babsorbs heat generated during rapid transfers
 vailability as either a complete stand-alone apparatus orA
a module compatible with the Mini-PROTEAN Tetra cell

For More Information
Download bulletin: 2033

2

4

3

Specifications
Maximum gel size (W x L)
Buffer requirement
Gel capacity
Recommended power supply
Dimensions (W x L x H)

10 x 7.5 cm
1.2 L
2 Mini-PROTEAN handcast gels or 2 Mini-PROTEAN precast gels
PowerPac HC (PowerPac Basic is a suitable alternative)
12 x 16 x 18 cm

Ordering Information
Catalog #Description

1703930

 ini Trans-Blot Electrophoretic Transfer Cell, includes 2 gel holder cassettes, 4 foam pads,
M
modular electrode assembly, blue cooling unit, lower buffer tank, lid with cables
Mini Trans-Blot Module, without lower buffer tank and lid
Mini-PROTEAN Tetra Cell and Mini Trans-Blot Module, includes 10-well, 1.0 mm, 4-gel system(#1658001) and blotting module (#1703935) without lower buffer tank and lid, gel casting accessories
Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply,
includes #1658001, #1703935, and #1645050
Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac BasicPower Supply, includes #1658004, #1703935, and #1645050
Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac HC
Power Supply, includes #1658004, #1703935, and #1645052
Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac HC Power Supply,
includes #1658001, #1703935, and #1645052

1703935*
16580291658033
1658034
16580361658035
Accessories
17039311703932
1703933
17038121703919
1703934
1651279

Mini Gel Holder CassetteThick Blot Paper, 7.5 x 10 cm, for Mini Trans-Blot cassette, 50 sheets
Foam Pads, 8 x 11 cm, 4
Mini Trans-Blot Central CoreBlue Cooling Unit, for Mini-PROTEAN Tetra tanks
Bio-Ice Cooling Unit, for Mini-PROTEAN 3 tanks
Roller, 3.5" wide

Price, U.S. $600.00485.001,280.00
1,515.00
1,375.00
1,850.00
2,060.00

96.00
48.00
39.00
385.00
31.00
50.00
39.00

* Also fits in the Mini-PROTEAN 3 electrophoresis cell.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 237

Western Blotting
Transfer Devices

See Also
PowerPac Basicand PowerPac HC
power supplies:
page 185.
Criterion precast
gels: page 202.
Blotting membranes:
page 242.
Blot detection
reagents: page 247.

bio-rad.com/tankblotters

Criterion Blotter
■

■

■

■

Blotting buffers:
page 245.
■■

 fficient transfers in 30 min to 1 hr for most proteins;Eovernight transfer at lower voltages is also an option
Plate electrodes, for faster and more efficient transfers,
or wire electrodes

Criterion blotter components:
1. Tank and lid.
2.	Assembly tray with gel holder
cassette, roller, foam pads, andblotting filter paper.
3. Wire electrodes.

4. Plate electrodes.
5. Sealed ice block.
6. Optional cooling coil.
1

Included assembly tray and roller ealed ice block provides sufficient cooling forS
most applications

2

6
5

Optional cooling coil — available for applications that
require precise temperature control
Cassettes with handles for easy removal from the tank

For More Information
Download bulletin: 2558

4

3

Specifications
Maximum gel size (W x L)
Buffer requirement
Gel capacity
Electrode choices
Recommended power supply
Dimensions (W x L x H)

15 x 9.4 cm
1.3 L
4 Mini-PROTEAN precast gels, 4 mini handcast gels, or 2 Criterion precast gels
Platinum-coated titanium anode and stainless-steel cathode plate electrodes,* or economical platinum wire electrodes
PowerPac HC
21.8 x 11.8 x 15 cm* Plate electrodes create a high-strength electrical field with higher current densities than other electrodes, producing faster and more efficient transfers.

Ordering Information
Catalog #Description

1704070

1703872
1703874

 riterion Blotter with Plate Electrodes, includes cell assembled with plate electrodes, lid with cables,C
2 Criterion gel holder cassettes, 1 pack precut blot absorbent filter paper, 4 foam pads, gel/blot assembly
tray, roller, sealed ice blockCriterion Blotter with Wire Electrodes, includes cell assembled with wire electrodes, lid with cables,
2 Criterion gel holder cassettes, 1 pack precut blot absorbent filter paper, 4 foam pads, gel/blot assembly
tray, roller, sealed ice blockCriterion Blotter with Plate Electrodes and PowerPac HC Power Supply, includes #1704070 and #1645052
Criterion Blotter with Wire Electrodes and PowerPac HC Power Supply, includes#1704071 and #1645052

1,705.00
1,610.00

Accessories
1704076
1704080
17040811704082
1704085
1704086
1704087
1704089
1651279

Optional Criterion Blotter Cooling CoilCriterion Blotter Gel Holder CassetteCriterion Blotter Platinum Anode Plate Electrode
Criterion Blotter Stainless-Steel Cathode Plate Electrode
Thick Blot Paper, 9.5 x 15.2 cm, for Criterion blotter, 50 sheets
Foam Pads, 9.5 x 15.2 cm, 4
Sealed Ice Blocks, for Criterion blotter, 2
Criterion Gel/Blot Assembly Tray
Roller, 3.5" wide

290.00110.00290.00160.00
51.00
61.00
44.00
73.00
39.00

1704071

238 | Electrophoresis and Blotting

Price, U.S. $845.00800.00In the U.S., call toll free at 1-800-424-6723

Western Blotting
Transfer Devices

bio-rad.com/tankblotters

See Also

Trans-Blot Cell Accessories

PowerPac HC
power supply:
page 185.

Accessories for the Trans-Blot transfer cell apparatus.
Trans-Blot cell accessories:
1. Gel holder cassette.
2. Foam pads.

Precast gels:pages 196, 202.

1

Blotting membranes:
page 242.
Blot detection
reagents: page 247.
Blotting buffers:
page 245.
2

Ordering Information
Catalog #Description

Accessories
17039141703956
1703960
1703913

Foam Pads, 15.5 x 20.5 cm, 6
Thick Blot Paper, 15 x 20 cm, for Trans-Blot cassette, 25 sheets
Extra Thick Blot Paper, 15 x 20 cm, 30 sheets
Gel Holder Cassette, includes 2 foam pads

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
50.00
48.00
61.00
100.00Electrophoresis and Blotting | 239

Western Blotting
Transfer Devices

bio-rad.com/microfiltration

Microfiltration and Screening Systems
Bio-Dot and Bio-Dot SF Microfiltration Apparatus
The 96-well Bio-Dot and 48-well Bio-Dot SF (slot format)
microfiltration units provide easy, reproducible methods
for binding proteins or nucleic acids in solution onto
membranes. The Bio-Dot SF apparatus focuses sample
to a thin line instead of a circle, making quantitation bydensitometry more reproducible. Each is available as acomplete, independent unit or as a modular template
without the manifold base. Features include:■

Resistance to 100% ethanol, strong acid, and NaOH■

Autoclavability■

Sealing gasket to eliminate lateral leakage■

Easy sample application with microplate-based spacing■

Flow valve (three-way) for adjustable vacuum

Dot format matches
96-well microplates

Modular design provides one baseplate for either Bio-Dot or Bio-Dot
SF template

Slot format allows easy
densitometric analyses todetermine relative
amounts of protein

Three-way vacuum valve allows sample
loading by gravity and quick washes

Specifications
FormatSamples
Sample volume
Well size
Quantitation withdensitometer
Overnight incubations
Membrane size (W x L)
Dimensions (W x H x D)
Weight

Bio-Dot Apparatus

Bio-Dot SF Apparatus

Dot blot
96-well, 8 x 12 format
50–600 µl
3 mm diameter
Yes, but Bio-Dot SF
unit recommended
Yes
12 x 9 cm
9 x 6.5 x 12"
1.1 kg (2.5 lb)

Slot blot
48-well, 6 x 8 format
50–500 µl
7 x 0.75 mm
Yes
No
12 x 9 cm
9.5 x 7 x 12"
1.2 kg (2.6 lb)

Ordering Information
Catalog #Description

1703938

1706543

 io-Dot Microfiltration System, includes Bio-Dot apparatus (#1706545) and Bio-Dot SF module (#1706543)
B
templates, vacuum manifold base, gasket support plates, gasket
Bio-Dot Apparatus, includes Bio-Dot sample template, vacuum manifold base, gasket support plate, gasket
Bio-Dot SF Apparatus, includes Bio-Dot SF sample template, vacuum manifold base,gasket support plate, gasket, filter paper
Bio-Dot SF Module, without vacuum manifold base, for conversion of Bio-Dot to Bio-Dot SF apparatus

Accessories
1706546
17065441620161

Bio-Dot Gaskets, 3
Bio-Dot SF Gaskets, 2
Bio-Dot/Bio-Dot SF Filter Paper, 11.3 x 7.7 cm, 60 sheets

17065451706542240 | Electrophoresis and Blotting

Price, U.S. $1,055.00
665.00665.00490.00
86.00
65.00
43.00

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Transfer Devices

bio-rad.com/microfiltration

Mini-PROTEAN II Multiscreen Apparatus
Advantages of the Mini-PROTEAN II multiscreen

apparatus include:■ uick and efficient screening or filtering of up to 40
Q
different antibodies or sera without cutting a western blot
into individual strips■

Compatibility with all common western blotting procedures■

Precise side-by-side comparison of results

■

■

■

■

■

 conomical use of antibody samples — requires onlyE
600 µl per channel
 eparate, detachable sample templates that accommodateS
one or two 8 x 7 cm blots
Clamps that secure the blot to form 40 leakproof channels
 olded gasket that eliminates cross-contaminationM
between samples
Easy operation and assemblySpecifications
Membrane size (W x L)
8 x 7 cm
Channel dimensions	2.5 mm x 5.2 cm x 5 mm
Dimensions (W x L x H)
27 x 11 x 6 cm

Ordering Information
Catalog #Description

17040171704018

Mini-PROTEAN II Multiscreen Apparatus, includes 2 sample templates, 2 gaskets, base plate
Multiscreen Gaskets, 2

Price, U.S. $785.0080.00

Mini Incubation Trays
Trays allow screening of antigens that have been blotted
onto membranes. An entire immunological screening
process can be carried out in a single tray.
■

Each tray has eight 10.5 cm x 5 mm channels toaccommodate strips cut from the blotted membrane.
Channels align with an eight-channel pipet■

Minimal reagent volumes needed (400 µl/channel)■

Numbered channels for sample identification■

Unique ribs in the tray lid and the design of the sample
channels ensure that no cross-contamination occurs

Ordering Information
Catalog #Description

1703902
1703903

Mini Incubation Trays, 20
Mini Incubation Trays, 100

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $120.00565.00Electrophoresis and Blotting | 241

Western Blotting
Membranes and Filter Papers

bio-rad.com/blottingmembranes

Membranes and Filter Papers
Bio-Rad offers a comprehensive line of blotting membranes including different grades of nitrocellulose and PVDF.
Use the selection guide below to choose the membrane appropriate for your application.
For More Information
Web: bio-rad.com/blottingmembranes
Blotting Membrane and Filter Paper Selection Guide

See Also
Filter paper:
page 244.

Binding Capacity, µg/cm2

MembranePore Size

Nitrocellulose

0.45 µm
80–1000.2 µm		Compatible Detection Methods

Notes

Colorimetric, chemiluminescence,chemifluorescence, radioactive

General-purpose protein blotting membrane

Supported
0.45 µm
80–100Colorimetric, chemiluminescence,
nitrocellulose
0.2 µm		chemifluorescence, radioactive				Pure nitrocellulose cast on an inert synthetic
support; increased strength for easier handlingand for reprobing

Immun-Blot
0.2 µm150–160
PVDF			High mechanical strength and chemical
stability; recommended for western blotting

Colorimetric, chemiluminescence,
radioactive

Immun-Blot
0.45 µm
155–300
Colorimetric, chemiluminescence,
LF PVDF			chemifluorescence, fluorescence				High mechanical strength and chemical
stability; low autofluorescence; recommended
for western blotting using fluorescence detection

Sequi-Blot
0.2 µm170–200
Colorimetric, radioactive
PVDF				High mechanical strength and chemical
stability; recommended for protein sequencing

Blotting Apparatus

Precut Membrane Sizes

Precut Filter Paper Sizes

Membrane/Filter Paper Sandwiches

Mini Trans-Blot cell

7 x 8.5 cm

7.5 x 10.5 cm

7 x 8.5 cm

Criterion blotter

8.5 x 13.5 cm

9.5 x 15.2 cm

8.5 x 13.5 cm

Trans-Blot cell

13.5 x 16.5 cm

15 x 20 cm—

Trans-Blot Plus cell		

25 x 28 cm
26.5 x 28 cm

—
—

—
—

Trans-Blot Turbo		

7 x 8.5 cm
8.5 x 13.5 cm

7.5 x 10.5 cm
9.5 x 15.2 cm

7 x 8.5 cm
8.5 x 13.5 cm (see page 235)

Trans-Blot SD cell
		
		
		
		

7 x 8.5 cm
11.5 x 16 cm
15 x 15 cm
15 x 9.2 cm
20 x 20 cm

7 x 8.5 cm
8 x 13.5 cm
14 x 16 cm
18 x 18.5 cm—

7 x 8.5 cm
8.5 x 13.5 cm———

Mini-PROTEAN II
multiscreen apparatus

7 x 8.5 cm
7 x 8.5 cm

7 x 8.5 cm—

7 x 8.5 cm—

Bio-Dot apparatus

9 x 12 cm

11.3 x 7.7 cm—

Bio-Dot SF apparatus

9 x 12 cm

11.3 x 7.7 cm—

Vacuum blotter

—

—

—

Nitrocellulose Membranes
Nitrocellulose
Nitrocellulose with the 0.45 µm pore size is recommended
for most analytical blotting including protein, ssDNA, andRNA transfers. For transfer of low MW proteins (<15 kD) or
nucleic acids, the 0.2 µm nitrocellulose membrane prevents
sample loss due to transfer through the membrane.
Supported Nitrocellulose
Made of 100% pure nitrocellulose cast on an inert synthetic
support, this nitrocellulose is a solid support for nucleic
acid and protein applications and can withstand the rigors
of multiple reprobing and autoclaving (121°C).

242 | Electrophoresis and Blotting

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Western Blotting
Membranes and Filter Papers

bio-rad.com/blottingmembranes

Ordering Information
Catalog #Description

Recommended Uses

Price, U.S. $Transfer of low MW
proteins or nucleic acids(has smaller pore size)

345.0069.00
97.00
200.00170.00235.00Transfer of low MW antigens,
immunoglobulins, glycoprotein
receptor proteins, histones
and nonhistones, etc.; capillary
Southern blotting of ssDNA and
RNA <500 bp

275.00
62.00
96.00
96.00
98.00
135.00120.00160.00Supported Nitrocellulose Membranes (0.2 µm)
1620097
Supported Nitrocellulose Membrane, 0.2 µm, 30 cm x 3 m, 1 rollProtein and nucleic acid blotting

340.00Supported Nitrocellulose Membranes (0.45 µm)
1620094
Supported Nitrocellulose Membrane, 0.45 µm, 30 cm x 3 m, 1 rollProtein and nucleic acid blotting

325.00

Nitrocellulose Membranes (0.2 µm)
1620112
Nitrocellulose Membrane, 0.2 µm, 30 cm x 3.5 m, 1 roll
1620146
Nitrocellulose Membranes, 0.2 µm, 7 x 8.4 cm, 10 sheets
1620168
Nitrocellulose Membranes, 0.2 µm, 8.5 x 13.5 cm, 10 sheets
1620147
Nitrocellulose Membranes, 0.2 µm, 13.5 x 16.5 cm, 10 sheets
1620150
Nitrocellulose Membranes, 0.2 µm, 20 x 20 cm, 5 sheets
1620252
Nitrocellulose Membranes, 0.2 µm, 26.5 x 28 cm, 10 sheets
Nitrocellulose Membranes (0.45 µm)
1620115
Nitrocellulose Membrane, 0.45 µm, 30 cm x 3.5 m, 1 roll
1620145
Nitrocellulose Membranes, 0.45 µm, 7 x 8.4 cm, 10 sheets
1620167
Nitrocellulose Membranes, 0.45 µm, 8.5 x 13.5 cm, 10 sheets
1620117
Nitrocellulose Membranes, 0.45 µm, 9 x 12 cm, 10 sheets
1620114
Nitrocellulose Membranes, 0.45 µm, 15 x 9.2 cm, 10 sheets
1620116
Nitrocellulose Membranes, 0.45 µm, 15 x 15 cm, 10 sheets
1620113
Nitrocellulose Membranes, 0.45 µm, 20 x 20 cm, 5 sheets
1620251
Nitrocellulose Membranes, 0.45 µm, 26.5 x 28 cm, 10 sheets

PVDF Membranes
The chemically resistant PVDF membrane has very highprotein binding capacity and resistance to tearing andcracking, even after repeated stripping and reprobing.
Immun-Blot PVDF for Western BlottingThis membrane is ideal for chemiluminescent andcolorimetric western blots because it retains target protein
very strongly but reduces nonspecific protein binding thatcan obscure high-sensitivity detection. Binding capacity is150–160 µg/cm2. The pore size of this membrane is 0.45 µm.
Sequi-Blot PVDF for Protein Sequencing
This membrane gives outstanding performance inprotein sequencing, even for low-abundance samples.
Sequi-Blot PVDF retains all transferred protein and has abinding capacity of 170–200 µg/cm2. The pore size of thismembrane is 0.2 µm.

In the U.S., call toll free at 1-800-424-6723

Immun-Blot Low Fluorescence PVDF Membrane
Optimized for fluorescence applications, the low
fluorescence property of the membrane enhances
image quality and improves sensitivity of all fluorescence
detection protocols. It is ideal for multiplex fluorescencewestern blotting and chemifluorescence applications.
The membrane is also compatible with other detection
methods such as chemiluminescence and colorimetricdetection. This membrane is highly recommended for the
Stain-Free western blotting workflow.
For More Information
Web: bio-rad.com/stainfreewestern
Download bulletins: 2212 and 6116

Electrophoresis and Blotting | 243

Western Blotting
Membranes and Filter Papers

bio-rad.com/blottingmembranes

Ordering Information
Catalog #Description

Price, U.S. $Immun-Blot PVDF Membranes
1620177
Immun-Blot PVDF Membrane, 26 cm x 3.3 m, 1 roll
1620174
Immun-Blot PVDF Membranes, 7 x 8.4 cm, 10 sheets
1620175
Immun-Blot PVDF Membranes, 10 x 15 cm, 10 sheets
1620176
Immun-Blot PVDF Membranes, 20 x 20 cm, 10 sheets
1620255
Immun-Blot PVDF Membranes, 25 x 28 cm, 10 sheets

325.0086.00
120.00250.00245.00Sequi-Blot PVDF Membranes
1620184
Sequi-Blot PVDF Membrane, 26 cm x 3.3 m, 1 roll

370.00Immun-Blot Low-Fluorescence PVDF Membranes
1620260
Low Fluorescence PVDF/Filter Paper Sandwiches, 7 x 8.5 cm, 10 pack1620261Immun-Blot Low Fluorescence PVDF/Filter Paper, 0.45 µm, 7 x 8.5 cm, 20 pack1620263
Immun-Blot Low Fluorescence PVDF/Filter Paper, 0.45 µm, 8.5 x 13.5 cm, 20 pack1620262
Low Fluorescence PVDF/Filter Paper Sandwiches, 8.5 x 13.5 cm, 10 pack1620264
Low Fluorescence PVDF Roll, 28 x 3.8 m, 1 roll

110.00170.00645.00Filter Paper
Bio-Rad offers a range of filter papers for blotting applications, including filter paper precut to fit standard gel sizes.
Ordering Information
Catalog #Description

Blot Absorbent Filter Paper (Extra Thick)1703965
Extra Thick Blot Paper, 7.5 x 10 cm, for Mini-PROTEAN
Tetra gels, 60 sheets
1703966
Extra Thick Blot Paper, 7 x 8.4 cm, for Mini-PROTEAN
Tetra gels, 60 sheets
1703967
Extra Thick Blot Paper, 8 x 13.5 cm, for Criterion precast gels, 60 sheets
1703968
Extra Thick Blot Paper, 14 x 16 cm, for PROTEAN II xi gels, 30 sheets
1703969
Extra Thick Blot Paper, 19 x 18.5 cm, for PROTEAN II XL gels, 30 sheets
1703958
Extra Thick Blot Paper, 10 x 15 cm, 30 sheets
1703959
Extra Thick Blot Paper, 15 x 15 cm, 30 sheets
1703960
Extra Thick Blot Paper, 15 x 20 cm, 30 sheets
Blot Absorbent Filter Paper (Thick)1703932
Thick Blot Paper, 7.5 x 10 cm, for Mini Trans-Blot cassette, 50 sheets
1704085
Thick Blot Paper, 9.5 x 15.2 cm, for Criterion blotter, 50 sheets
1703955
Thick Blot Paper, 14 x 16 cm, for PROTEAN II xi gels, 25 sheets
1703956
Thick Blot Paper, 15 x 20 cm, for Trans-Blot cassette, 25 sheet
1620161
Bio-Dot/Bio-Dot SF Filter Paper, 7.7 x 11.3 cm, 60 sheets
Blot Absorbent Filter Paper (Thin)
1620118
Thin Blot Paper, 33 cm x 3 m, 1 roll

Recommended Uses

Price, U.S. $All blotting applications using theTrans-Blot SD cell or Trans-Blotcell (precut to gel dimensions fromwell to bottom of gel)

56.00
56.00
56.00
59.00
61.00
56.00
59.00
61.00

All blotting applications requiring
thick, high wet strength filter paper

48.00
51.00
50.00
48.00
43.00

All blotting applications requiring
thin, high wet strength filter paper

43.00

Blotting Stains and Tracking Dyes
Bio-Rad offers a selection of stains for blotting applications; see page 253. Tracking dyes can be found on page 264.

244 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Blotting Buffers and Blocking Reagentsbio-rad.com/blotting

Blotting Buffers and Blocking Reagents
VideosEveryBlot Blocking Buffer

Product
Demonstration

Proper blocking of membranes before immunodetection
is critical for reducing nonspecific binding. An effective
blocker minimizes background and allows sensitive
detection of target proteins.
EveryBlot blocking buffer benefits include:

bio-rad.com/
EB5minutes

Complete blocking in 5 min■ ompatible with all detection methods and can beCused for detection of phosphorylated targets■

 ow background and high signal on fluorescent andLchemiluminescent blots■

For More Information
Web: bio-rad.com/everyblot
Request or download bulletin: 7185

Ordering Information
Catalog #Description

12010020

EveryBlot Blocking Buffer, 500 ml, high performance blocking buffer for all western blots; blocks in 5 min

Price, U.S. $325.00Premixed Blotting Buffers and Buffer Reagents
Two transfer buffers are available: 10x Tris/glycine and 10x Tris/CAPS. Premixed blocking buffers, available as 1x PBS
with casein and 1x TBS with casein, take the time and effort out of solubilizing casein. Bio-Rad offers a complete line ofreagents for preparation of buffers to your own specifications.
Blotting Buffer Selection Guide			

1x Formulation

Applications

Transfer Buffers*
10x Tris/glycine

25 mM Tris, 192 mM glycine, pH 8.3

Western blotting

10x Tris/CAPS			

Anode buffer: 60 mM Tris,
40 mM CAPS, 15% methanol, pH 9.6

A discontinuous buffer system that increases
transfer efficiency in semi-dry applications

			
			

Cathode buffer: 60 mM Tris,
40 mM CAPS, 0.1% (w/v) SDS, pH 9.6

Processing Buffers
10x PBS			

10 mM sodium phosphate,
150 mM NaCl, pH 7.4

Western blotting wash solution

10x TBS

20 mM Tris, 500 mM NaCl, pH 7.4

Western blotting wash solution

1x PBS with 1% casein
10 mM sodium phosphate,			
150 mM NaCl, 1% (w/v) casein, pH 7.4
				

Western blotting blocking buffer (casein
blockers recommended for all applications,
including those with biotin-avidin complexes)

1x TBS with 1% casein
20 mM Tris, 500 mM NaCl,			
1% (w/v) casein, pH 7.4
				

Western blotting blocking buffer (casein
blockers recommended for all applications,
including those with biotin-avidin complexes)* These buffers can be used for all gel types and formulations.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 245

Western Blotting
Blotting Buffers and Blocking Reagentsbio-rad.com/blotting

Ordering Information
Catalog #Description

Price, U.S. $Blot Transfer and Processing Buffers
1610734
10x Tris/Glycine, 1 L
1610771
10x Tris/Glycine, 5 L cube
1610778
10x Tris/CAPS, 1 L
1610780
10x Phosphate Buffered Saline, 1 L

28.00
94.00
74.00
44.00

1706435

40.00

Reagents
1610610**1610611**1610729
1706537
16107171610718

10x Tris Buffered Saline, 1 L

Dithiothreitol (DTT), 1 g
Dithiothreitol (DTT), 5 g
EDTA, 500 g
Gelatin, EIA grade, 200 g
Glycine, 250 g
Glycine, 1 kg

27.00
81.00
72.00
56.00
24.00
53.00

Catalog #Description

Detergents and Blocking Buffers
1706404
Blotting-Grade Blocker,
nonfat dry milk, 300 g
1706531
Tween 20, EIA grade, 100 ml
161078110% (w/v) Tween 20, for easy
pipetting, 1 L
1610418
SDS Solution, 20% (w/v), 1 L
1610783*
1x Phosphate Buffered Salinewith 1% Casein, 1 L
1610782*
1x Tris Buffered Saline with 1%
Casein, 1 L
16107101632101
1610713161071616107191610730

2-Mercaptoethanol, 25 ml
Tributylphosphine (TBP),
200 mM, 0.6 ml
Tricine, 500 g
Tris, 500 g
Tris, 1 kg
Urea, 250 g

Price, U.S. $
47.00
53.00
36.00
120.00
94.00
94.00

23.00
44.00
210.00
33.00
84.00
27.00

* Store at 2–8°C.
** Store desiccated at 2–8°C; store other reagents at room temperature, dry, and away from direct sunlight.Hazardous shipping charges may apply.

246 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Immunodetection Reagents and Kits

bio-rad.com/blotdetection

Immunodetection Reagents and Kits
The most common blot detection techniques use antibodies to either probe for specific antigens in a complex protein
sample or stain all proteins bound to a membrane. The chart indicates the maximum sensitivity achievable with eachdetection system.
For more information on methods, equipment, and reagents used in protein blotting, download the Protein Blotting Guide (bulletin 2895).

Key
HRP
AP

Horseradish peroxidase
Alkaline phosphatasew
w
Coomassie
Brilliant Blue R-250

Colloidal
Gold Stain

TransferAe
Onto M

Clarity
Clarity Max
Max
Western
Western ECLECLSubstrate
Substrate

ClarityWestern ECL
Substrate

Immun-Star
AP KitAmplified
Opti-4CN Kit

Opti-4CN Kit

DABImmun-Blot ®
AP KitBlot detection reagent selection guide.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 247

Western Blotting
Immunodetection Reagents and Kits

bio-rad.com/blotdetection

Chemiluminescence Detection
Chemiluminescent western blot detection offers highly sensitive detection of proteins bound to blotting membranes.
Most specific antigen detection methods are based on HRP (horseradish peroxidase) or AP (alkaline phosphatase)secondary antibody conjugates. The signal can be captured with film or dedicated imaging equipment(see page 284).
Chemiluminescence-Based Kit Selection Guide			Clarity Western ECLClarity Max Western ECL

Immun-Star AP

Lower detection limit

Mid femtogramLow femtogram

10 pg

Signal duration

24 hr

2 hr

24 hr

Primary detection method

Digital imager and film

Digital imager and film

Film

Suggested anitbody dilution			Primary: 1:1,000–1/50,000
Secondary: 1:50,000–1/250,000

Primary: 1:1,000–1/50,000
Secondary: 1:50,000–1/250,000

Primary: 1:1,000–1/6,000
Secondary: 1:3,000

Recommended membrane

Nitrocellulose, PVDF, or LF PVDF

Nitrocellulose, PVDF, or LF PVDF

Nitrocellulose, PVDF, or LF PVDF

Clarity and Clarity Max Western ECL Substrates
Clarity Western ECL Substrate
Clarity Western ECL substrate is compatible with any
HRP-conjugate secondary detection reagent and ideal forboth digital and film-based imaging. The Clarity substrateprovides excellent sensitivity with an extremely long signal
duration that allows re-imaging without loss of signal.
Features include:■

Low background levels, yielding very clear images■

Bright, long signal■

Shelf life of 12 months at room temperature

Clarity Max Western ECL Substrate
Clarity Max western ECL substrate is our brightest ECL
substrate, providing additional sensitivity when you need
it most. Clarity Max is perfect for blots involving lowabundance or otherwise difficult-to-detect targets. This
ECL substrate completes our western ECL substrateoffering and fits right into your current western blotting
process. Use it in place of your current ECL substrate when
you need additional sensitivity. Features include:■

Room temperature storage■

Low–femtogram level sensitivity■

Compatibility with HRP conjugates

Clarity and Clarity Max Western ECL Substrates

For More Information
Web: bio-rad.com/clarity
Request or download bulletin: 6305

Note: For research use only. Not for diagnostic procedures.
Ordering Information
Catalog #Description

1705060

 larity Western ECL Substrate, 200 ml size contains Clarity western peroxide reagent,C
100 ml, and Clarity western luminol/enhancer reagent, 100 ml

1705061

Clarity Western ECL Substrate, 500 ml size contains Clarity western peroxide reagent,
250 ml, and Clarity western luminol/enhancer reagent, 250 ml
Clarity Max Western ECL Substrate, 100 ml, contains 50 ml Clarity Max western peroxide
reagent and 50 ml Clarity Max western luminol/enhancer reagent

1705062

248 | Electrophoresis and Blotting

Price, U.S. $135.00

255.00In the U.S., call toll free at 1-800-424-6723

Western Blotting
Immunodetection Reagents and Kits

bio-rad.com/blotdetection

Immun-Star AP Chemiluminescence Kits
These kits combine Bio-Rad’s blotting reagents and
CDP-Star chemiluminescence technology. Exposure times
on film are typically between 30 seconds and 5 minutes,
depending on sample amount and antibody specificity.
Immun-Star AP kit features include:■ he ability to reactivate a blot, even weeks later, withT
the addition of fresh chemiluminescent substrate■

Detection of as little as 10 pg of protein■

Stable light signal duration of 24 hr■

Ability to strip and reprobe
For More Information
Web: bio-rad.com/blotdetection
Download bulletin: 2050

Choose from two kits based on goat anti-mouse or goatanti-rabbit conjugates.
Ordering Information
Catalog #Description

Immun-Star AP Kits** and Components
1705010GAM-AP Detection Kit
1705011GAR-AP Detection Kit
1705018
AP Substrate, 125 ml
1705012AP Substrate Pack
1705014Goat Anti-Rabbit (GAR)-AP Intro Kit

Price, U.S. $Substrate

Enhancer*

Antibody

•
•
•
•
•

•
•

•
•

315.00315.00260.00•••290.00* The enhancer is used on nitrocellulose blots but is not optimized for PVDF blots. Additional testing is recommended to determine
appropriate conditions for PVDF blots.
** GAM, goat anti-mouse; GAR, goat anti-rabbit.

Colorimetric Detection
Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on theblot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over backgroundis observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however,
the method’s single end-point result does not allow multiple exposures of chemiluminescent methods. Colorimetricdetection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.
However, Bio-Rad offers amplified colorimetric systems that provide high sensitivity comparable to or exceeding that ofchemiluminescent detection.
For More Information
Web: bio-rad.com/blotdetection

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 249

Western Blotting
Immunodetection Reagents and Kits

bio-rad.com/blotdetection

Colorimetric HRP Detection
Bio-Rad offers three types of kits based on the detection
reagent 4-chloro-1-naphthol (4CN) for colorimetric HRP
detection; individual reagents are also available, including
3,3'-diaminobenzidine (DAB), an alternative reagent.
For More Information
Download bulletin: 2260

Opti-4CN Substrate and Detection Kits
Detection sensitivity using 4CN is about 500 pg of antigen,with the benefit of very low background. The Opti-4CN kit
improves detection sensitivity over that of 4CN, to 100 pg,
with no additional steps required.
Amplified Opti-4CN Substrate and Detection Kits
Amplified Opti-4CN detection kits are based on proprietary
HRP-activated amplification reagents from Bio-Rad.
These kits allow colorimetric detection to 5 pg, which iscomparable to chemiluminescence detection sensitivity.
No additional materials or special equipment are required.

Immun-Blot HRP Assay Kits
Immun-Blot HRP assay kits provide the reagents required
to perform standard HRP/4CN colorimetric detection onwestern blots with the added convenience of premixed
buffers and enzyme substrates. All kit components areindividually tested for quality control in blotting applications.
Premixed and Individual HRP Colorimetric Substrates
Premixed enzyme substrate kits are convenient and reliable
and reduce exposure to hazardous reagents used in thecolor development of western blots.

Ordering Information
Catalog #Description

Price, U.S. $Opti-4CN Kits*
1708235
Opti-4CN Substrate Kit

175.00

Amplified Opti-4CN Kits*
1708238
Amplified Opti-4CN Substrate Kit
1708240
Amplified Opti-4CN Goat Anti-Mouse Detection Kit

420.00480.00Immun-Blot HRP Assay Kits, with 4CN**
1706463
Goat Anti-Rabbit IgG (H + L)-HRP Assay Kit
1706464
Goat Anti-Mouse IgG (H + L)-HRP Assay Kit
1706465
Goat Anti-Human IgG (H + L)-HRP Assay Kit

250.00
255.00250.00Premixed Substrate Reagents
HRP Conjugate Substrate Kit, contains premixed 4CN, hydrogen peroxide solutions,
1706431
color development buffer; makes 1 L color development solution
Individual Blotting Substrates
1706534
HRP Color Development Reagent, 4CN, 5 g
1706535
HRP Color Development Reagent, DAB, 5 g

110.0056.00
145.00*		Each kit contains enough reagent for 2,500 cm2 of membrane or approximately 50 mini blots.
** Kits contain 0.5 ml of specific HRP blotting-grade conjugate; each kit provides reagents (blotting-grade TBS buffer,Tween 20 detergent, gelatin blocking reagent, and 4CN substrate solution) for 200 assays on a 0.6–0.8 x 9.2 cm
nitrocellulose strip using a total volume of 5.0 ml.

250 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Western Blotting
bio-rad.com/blotdetection

Immunodetection Reagents and Kits

Colorimetric AP Detection
Immun-Blot AP Assay Kits
A common substrate for colorimetric detection on western
blots based on AP-conjugated secondary antibodies
is 5-bromo-4-chloro-3-indolyl phosphate/nitroblue
tetrazolium (BCIP/NBT). Immun-Blot AP assay kits provide
the essential reagents to perform colorimetric detection(of up to 100 pg of protein) based on AP and BCIP/NBT
with the added convenience of premixed buffers andenzyme substrates. All kit components are individuallytested for quality control in blotting applications.
Premixed AP Colorimetric Substrates
Premixed enzyme substrate kits provide convenience andreliability and reduce exposure to hazardous reagents.
For More Information
Web: bio-rad.com/blotdetection
Download bulletins: 1600 and 2032

Ordering Information
Catalog #Description

Price, U.S. $Opti-4CN Kits*
1706460
Immun-Blot Goat Anti-Rabbit IgG (H + L)-AP Assay Kit
1706461Immun-Blot Goat Anti-Mouse IgG (H + L)-AP Assay Kit

295.00295.00Premixed Substrate Reagents
AP Conjugate Substrate Kit, contains premixed BCIP and NBT solutions and color development buffer;1706432
makes 1 L color development solution

155.00*K its contain 0.5 ml of specific AP blotting-grade conjugate; each kit provides reagents (blotting grade TBS buffer,Tween 20 detergent, gelatin blocking reagent, and BCIP and NBT substrate solution) for 200 assays on a 0.6–0.8 x 9.2 cm
nitrocellulose strip using a total volume of 5.0 ml.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 251

Western Blotting
Immunodetection Reagents and Kits

bio-rad.com/blotdetection

Western Blot Conjugates and Reagents
Blotting-Grade Conjugates and ReagentsPrimary Antibodies
For our selection of primary antibodies for western blotting
and other applications, see page 272.

Protein A and Protein G Conjugates
Proteins A and G are bacterial cell surface proteins thatbind to the Fc region of IgG molecules. Each reagent has
different IgG binding capabilities, depending on the speciesof origin of the immunoglobulin.

Secondary Antibodies
For our wide range of secondary antibodies for western
blotting, ELISA, IHC, IF, and flow cytometry, see page 278.

Blotting-Grade Reagents
Detergents and blocking reagents for western blotting areavailable individually.

For More Information
Web: bio-rad.com/blotdetection

Binding Specificities of Protein A and Protein G Conjugates
Immunoglobulin

Protein A

Protein G

•
•
—
•
° /—
•

•
•
•
•

Human IgG1
Human IgG2
Human IgG3
Human IgG4
Mouse IgG1
Mouse IgG2a• Strong binding.

° Weak binding.

Immunoglobulin°•

Mouse IgG2b
Mouse IgG3
Rat IgG1
Rat IgG2a
Rat IgG2b
Rat IgG2c

Recommended Dilution

Products

Protein A

Protein G

•
•

•
•

°
—
—
•

°
•
°
•

— No binding.

Conjugate Specifications*
Products

Volume, ml

Volume, ml

Recommended Dilution

Goat anti-rabbit lgG-AP
Goat anti-mouse lgG-AP
Goat anti-human lgG-AP
Avidin-AP
Biotinylated-GAR (H + L),
human lgG adsorbed

1
1
1
1
1

1:3,000
1:3,000
1:3,000
1:1,000–1:3,000
1:3,000

Price, U.S. $Catalog #Description

210.0017065221706425
1706528

Protein A-HRP, 1 ml
Protein G-HRP, 1 ml
Avidin-HRP, 2 ml

170.00150.00115.00Immun-Star GAR
2
1:10,000
Immun-Star GAM
2
1:10,000
Avidin-HRP2
1:1,000–1:3,000
Goat anti-rabbit (H + L)
2
1:3,000
Goat anti-mouse (H + L)
2
1:3,000
Goat anti-human (H + L)
2
1:3,000
Protein A-HRP
1
1:3,000
Protein G-HRP
1
1:3,000* Shelf life of conjugates is one year when stored at 4°C.

Ordering Information
Catalog #Description

Price, U.S. $Blotting-Grade Conjugates, HRP
1705046
Immun-Star Goat Anti-Rabbit(GAR)-HRP, lyophilized
1705047
Immun-Star Goat Anti-Mouse (GAM)HRP, lyophilized
1706515
Goat Anti-Rabbit IgG (H + L)-HRP, 2 ml
1706516
Goat Anti-Mouse IgG (H + L)-HRP, 2 ml
1721050
Goat Anti-Human IgG (H + L)-HRP, 2 ml

185.00185.00160.00Blotting-Grade Conjugates, AP
1706518
Goat Anti-Rabbit IgG-AP, 1 ml
1706520
Goat Anti-Mouse IgG-AP, 1 ml

190.00190.001706521

Goat Anti-Human IgG-AP, 1 ml

185.00170.00

56.00
47.00

1610418
1610783*

120.00
94.00

53.00
36.00

1610782*

SDS Solution, 20% (w/v), 1 L
1x Phosphate Buffered Salinewith 1% Casein, 1 L
1x Tris Buffered Saline with
1% Casein, 1 L

Detergents and Blocking Reagents
1706537
Gelatin, EIA grade, 200 g
1706404
Blotting-Grade Blocker, nonfat dry milk,
300 g
1706531
Tween 20, EIA grade, 100 ml
1610781
10% Tween 20, for easy pipetting, 1 L

210.0094.00* Store at 2–8°C.

252 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Western Blotting
Immunodetection Reagents and Kits

bio-rad.com/blotdetection

Total Protein Blot Detection
Bio-Rad offers three stain options for total protein detection. For electrophoresis stains, see page 221.

Total Protein Stains
Comparison of Total Protein Staining Methods
Stain

Sensitivity

Staining Time

Advantages

Disadvantages

Coomassie Brilliant Blue R-250
100–1,000 ng~1 hr
Inexpensive, rapid stain
Low sensitivity, shrinks					nitrocellulose membranes
Colloidal gold total protein stain
1 ng
~2 hr
Very sensitive, rapid stain
High background with					nylon membranes

Coomassie Brilliant Blue R-250 Dye
Coomassie Brilliant Blue R-250 is an anionic dye used forstaining gels and membranes (PVDF and nitrocellulose).
It is a rapid and inexpensive stain that can detect
nanogram levels of protein. Since this dye can interferewith antibody binding sites, subsequent detection ofproteins by immunostaining is not recommended.
Colloidal Gold Total Protein Stain
Colloidal gold total protein stain is a stabilized gold stain
optimized for rapid and sensitive identification of proteinsbound to nitrocellulose membranes (Rohringer and Holden
1985). Protein bands stain dark red following incubationof the membrane with colloidal gold solution. The stained
membrane yields a permanent record of the protein pattern
for exact comparison to immunostained results. Colloidal
gold total protein stain is provided ready to use.

Coomassie Brilliant Blue stain.

Colloidal gold total protein stain.

For More Information
Web: bio-rad.com/totalprotein

Ordering Information
Catalog #Description

1610400
1706527

Coomassie Brilliant Blue R-250, 10 g
Colloidal Gold Total Protein Stain, 500 ml

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $20.00
150.00Electrophoresis and Blotting | 253

Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

bio-rad.com/DNAelectro

Nucleic Acid Electrophoresis and Blotting
Bio-Rad offers a wide range of nucleic acid electrophoresis and blotting tools for life science research — from molecularladders to mutation detection systems. Different system formats and sizes are available to accommodate a variety of
application needs.
Learn More about the Technology
Web: bio-rad.com/tech/DNAelectro

See Also
Certified agaroses:
page 266.
PowerPac Basic
power supply:
page 185.
DNA ladders:page 267.
Premixed runningbuffers: page 265.

DNA Electrophoresis Systems
Bio-Rad offers a complete line of easy-to-use horizontal
agarose gel electrophoresis systems, varying in length
and width, for both low- and high-throughput applications.
Submerged horizontal electrophoresis cells include threemodels that can run precast or handcast gels:■

Nucleic acid reagents:
page 264.

Mini-Sub Cell GT cell■

Wide Mini-Sub Cell GT cellGel documentation
systems: page 284.

■

Sub-Cell GT cell

ReadyAgarose
precast gels:page 260.

And two models configured to run ReadyAgarose
precast gels:■

Mini ReadySub-Cell GT cell■

Wide mini ReadySub-Cell GT cell

ElectricalcablesSafety lid

Electrical
leads

Comb
slots

Fixed-heightcomb

Fluorescent
ruler
UV-transparent
gel trayBanana plug/
electrodewire assembly

Gel castinggates

Key features of the Sub-Cell family include:■

UV-transparent gel trays with an integrated fluorescent ruler■

Multiple options for hand casting gels of different sizes■

Combs to fit virtually every need■

Clear plastic construction for easy sample visualization■

 uffer recirculation ports for applications that requireBhigh voltages or extended runs■

Easy-to-replace electrode cassettes■

IEC 1010 (EN 61010) electrical safety certification

Mini-Sub Cell
GT baseGel stage
Safety lid removal tab

Components of the Mini-Sub Cell GT cell.

For More Information
Web: bio-rad.com/subcell
Request or download bulletin: 2660

254 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

bio-rad.com/DNAelectro

Sub-Cell Family Selection Guide

				
Cell size, cm (W x L x H)

Mini-Sub Cell GT and
Mini ReadySub-Cell GT*

Wide Mini-Sub Cell GT and
Wide Mini ReadySub-Cell GT**

Sub-Cell GT

9.2 x 25.5 x 5.6

17.8 x 25.5 x 6.8

18 x 40.5 x 9.4

Gel tray sizes, cm (W x L)
7x7
15 x 7		
7 x 1015 x 10								
ReadyAgarose gelsaccommodated

Yes (mini format:
8-, 12-, 2 x 8-well)Yes (wide mini format: 20-, 32-,
2 x 32-well; 96 Plus format: 4 x 26-well)

15 x 1015 x 1515 x 2015 x 25
No

Sample
8–30***
10–60***
1–120†
throughput				Base buffer
volume, L

~0.27

~0.65

~1.0

Distance to electrode, cm

14.5

14.5

29.5

Bromophenol
blue migration at 75 V

~4.5 cm/hr~4.5 cm/hr~3.0 cm/hr*	The mini ReadySub-Cell GT cell is a Mini-Sub Cell GT cell dedicated to running ReadyAgarose precast gels, gel size 7 x 10 cm;
sample throughput is 8-, 12-, or 2 x 8-well. This cell does not include casting gates, tray, or combs.
**	The wide mini ReadySub-Cell GT cell is a wide Mini-Sub Cell GT cell dedicated to running ReadyAgarose precast gels, gel size 15 x 10 cm;
sample throughput is 20-, 32-, 2 x 32-, or 4 x 26-well. This cell does not include casting gates, tray, or combs.
*** Sample throughput value assumes 1–2 combs per gel.
†

Sample throughput value assumes 1–4 combs per gel.

Mini-Sub Cell GT Cells
 he redesigned Mini-Sub Cell GT cell offers updatedTfeatures that make electrophoresis even easier. A Mini-Sub
Cell GT cell can resolve up to 30 samples. Its short, narrowformat allows 7 and 10 cm runs with speed, simplicity, andeconomy. These cells resolve EcoRI or HindIII digests oflambda phage DNA in only 1.5 hours at 60 V. Small DNA
fragments can be separated in as little as 15 minutes at
150 V. All mini cells accommodate ReadyAgarose precastgels and include a buffer tank, safety lid with cables, andleveling bubble.
T he mini ReadySub-Cell GT cell (#1704487) is identicalto the Mini-Sub Cell GT cell, except it is dedicatedto running mini ReadyAgarose precast gels. This cell
does not include casting gates, tray, or combs. These
components are available to upgrade the ReadySub-Cell
GT cell for handcasting capability.
For More Information
Web: bio-rad.com/subcell

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 255

Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

bio-rad.com/DNAelectro

Ordering Information
UV-Transparent Tray, cm
Catalog #7 x 7*

Mini-Sub Cell GT Systems**
1704406•
1704486•
1640300
1704467
1704466
1704487
Catalog #Combs

7 x 108-Well

15-Well

•
•
•

•
•
•
•
•

•
•
•
•
•

Mini Casting
Gates

Mini
Gel Caster••

PowerPac BasicPower Supply(#1645050)

•
•
•

•

Description

Price, U.S. $Mini-Sub Cell GT Accessories
1704491
Mini-Sub Cell GT Handcasting Kit, includes 7 x 7 cm UV-transparent tray, casting gates, 8- and 15-well
1.5 mm fixed-height combs
1704422
Mini-Sub Cell GT Gel Caster, for Mini-Sub Cell GT and wide Mini-Sub Cell GT systems
1704436*
Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 7 cm (trays have 2 slots for fixed-height combs)
1704435
Mini-Sub Cell GT UV-Transparent Gel Tray, 7 x 10 cm (trays have 2 slots for fixed-height combs)
1704330**
Original UV-Transparent Mini-Gel Tray, 7 x 10 cm
1704434
Mini-Sub Cell GT Casting Gates
1704362
Mini-Sub Cell GT Anode (Red) QuickSnap Electrode Assembly
1704363
Mini-Sub Cell GT Cathode (Black) QuickSnap Electrode Assembly

Catalog ## of Wells

Combs for Mini-Sub Cell GT Systems
1704464151704465***15170433215
170446281704463***81704333
8
2 preparative
1704461
2 reference
1 preparative
1704460
2 reference

Height†Thickness, mm

Fixed
Fixed
Adjustable
Fixed
Fixed
Adjustable

0.75
1.5
1.0
0.75
1.5
1.0

Fixed

1.5

Fixed

1.5

Width ofWell, mm
3
2.6
2.6
5.5
5.5
5.5
20.0
4.0
44
3.0

Length of Teeth, mm
10
10.2
10.2
11.0
11.0
10.2
10
10.2

150.00
88.00
57.00
56.00
86.00
93.00
75.00
75.00

Volume, µl(in 5 mm deep gel)
11.25
19.1
13.0
20.0
40.0
27.5
150
30.0
330
22.5

Price, U.S. $
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00

* 		Allows casting gels in the cell using casting gates; 7 x 10 cm gels can be cast with a gel caster.
**		Mini-Sub Cell systems purchased before 1996 (Mini-Sub DNA cell) require casting tray #1704330. This tray is not compatible with the MiniSub Cell GT system.
*** Combs included in systems.
† 			Fixed-height combs must be used with Mini-Sub Cell GT system gel trays. Adjustable-height combs require comb holder, #1704331.

256 | Electrophoresis and Blotting

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Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

bio-rad.com/DNAelectro

Wide Mini-Sub Cell GT Cells
 he redesigned wide Mini-Sub Cell GT cell offers updatedTfeatures that make electrophoresis even easier for multiple
samples and rapid screening applications. This popular
system has a wide platform that can separate 30 samples
per comb. The wide Mini-Sub Cell GT cell is the same
width as the Sub-Cell GT cell, so the comb holders,
combs, and 15 x 10 cm gel trays are interchangeablewith the larger Sub-Cell GT units. All wide mini cells
accommodate ReadyAgarose precast gels and include
a buffer tank, safety lid with cables, and leveling bubble.
 he wide mini ReadySub-Cell GT cell (#1704489) isTidentical to the wide Mini-Sub Cell GT cell, except it
is dedicated to running ReadyAgarose precast gels.
This cell does not include casting gates, tray, or combs.
These components are available to upgrade the wide mini
ReadySub-Cell GT cell for handcasting capability.
For More Information
Web: bio-rad.com/subcell

Ordering Information
UV-Transparent
Tray, cm
Catalog #15 x 7*15 x 10

Wide Mini-Sub Cell GT Systems**
1704405•
1704485•
1704468•
1704469•
1640301•
1704489
Catalog #Combs
15-Well

20-Well

•
•
•
•
•

•
•
•
•
•

Wide Mini
Casting
Gates

Mini Gel
Caster•••••PowerPac Basic Power
Supply (#1645050)•

Description

Wide Mini-Sub Cell GT Accessories
1704497
Wide Mini-Sub Cell GT Handcasting Kit, includes 15 x 7 cm tray, casting gates, 15- and 20-well
1.5 mm fixed-height combs, 20-well 1.5 mm fixed-height comb
1704422
Mini-Sub Cell GT Gel Caster, for Mini-Sub Cell GT and wide Mini-Sub Cell GT systems
1704426
Wide Mini-Sub Cell GT UV-Transparent Gel Tray, 15 x 7 cm (trays have 2 slots for fixed-height combs)
1704416**,***
Sub-Cell GT UV-Transparent Gel Tray, 15 x 10 cm (trays have 2 slots for fixed-height combs)
1704425
Wide Mini-Sub Cell GT Casting Gates
1704371
Wide Mini-Sub Cell GT Cell Replacement Lid with Cables
1704372
Wide Mini-Sub Cell GT Anode (Red) QuickSnap Electrode Assembly
1704373
Wide Mini-Sub Cell GT Cathode (Black) QuickSnap Electrode Assembly* 			Allows casting gels in the cell using casting gates; 15 x 10 cm gels can be cast with a gel caster.
** 		Tray is compatible with the Sub-Cell DNA system.
*** 15 x 10 cm gel tray can be used for both wide Mini-Sub Cell GT and Sub-Cell GT cells.

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $505.00525.00575.00575.00965.00455.00Price, U.S. $170.00
88.00
84.00
95.00
94.00
61.00
61.00

Electrophoresis and Blotting | 257

Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

bio-rad.com/DNAelectro

Sub-Cell GT Cell
The redesigned Sub-Cell GT cell is the most versatilehorizontal electrophoresis cell in the Sub-Cell family,
offering the greatest choice of gel lengths, combs, andseparation modes that make it ideal for Southern andnorthern blotting protocols. Up to 30 samples can be
resolved over a distance of 25 cm. Using four rows ofcombs, the cell can run up to 120 samples. All Sub-Cell
GT units include a buffer tank, safety lid with cables,leveling bubble, and combs (15- and 20-well). System
configurations that include additional accessories arealso available.
For More Information
Web: bio-rad.com/subcell

Ordering Information
UV-Transparent Tray, cm
Catalog #15 x 10 15 x 15* 15 x 20 15 x 25

Sub Cell GT Systems**
1704401•
1704481•1704402
1704482
1640302
1704403
1704483
1704404
1704484
Catalog #

•
•
•
•
•
•
•

Combs
15-Well

20-Well

•
•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•

Casting
Gates

Gel
CasterPowerPac BasicPower Supply (#1645050)

•
•
•
•

•
•

•

•
•

Description

Price, U.S. $670.00670.00685.00670.00735.00735.00735.00735.001,130.00
Price, U.S. $Sub-Cell GT Accessories
1704412Sub-Cell GT Gel Caster, for Sub-Cell GT Systems
1704416**
Sub-Cell GT UV-Transparent Gel Tray, 15 x 10 cm (trays have 2 slots for fixed-height combs)
1704417*
Sub-Cell GT UV-Transparent Gel Tray, 15 x 15 cm
1704418
Sub-Cell GT UV-Transparent Gel Tray, 15 x 20 cm
1704419
Sub-Cell GT UV-Transparent Gel Tray, 15 x 25 cm
1704415
Sub-Cell GT Casting Gates
1704392
Sub-Cell GT Anode (Red) QuickSnap Electrode Assembly
1704393
Sub-Cell GT Cathode (Black) QuickSnap Electrode Assembly

115.0095.00
110.00110.00110.00
98.00
88.00
88.00

*		Allows casting gels in the cell using casting gates. Other gel sizes can be cast with a gel caster.
** 15 x 10 cm gel tray can be used for both Sub-Cell GT and wide Mini-Sub cell GT cells.

258 | Electrophoresis and Blotting

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Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

bio-rad.com/DNAelectro

Combs for Wide Mini-Sub Cell and Sub-Cell GT Cells

Ordering Information
Catalog ## of Wells

Fixed and Adjustable Combs
170444930
170434430
170444720
1704448**20
1704321201704322201704445151704446**15
170432315
1704324
15
170444310170444410170432510
1704326
10HeightThickness, mm

Width ofWell, mm

Length ofTeeth, mm

Volume, µl(in 5 mm deep gel)

Fixed
Adjustable
Fixed
Fixed
Adjustable
Adjustable
Fixed
Fixed
Adjustable
Adjustable
Fixed
Fixed
Adjustable
Adjustable

1.5
1.5
0.75
1.5
0.75
1.5
0.75
1.5
0.75
1.5
0.75
1.5
0.75
1.5

3
2.7
5
5
4.8
4.8
6
6
5.5
5.5
10
10
9.9
9.9

15
19.1
15
15
19.1
19.1
15
15
19.1
19.1
14
14
19.1
19.1

22.5
20
18.75
37.5
17
34
22.5
45
21
42
37.5
75
37
74

Fixed

1.5

Fixed

1.5

Fixed

1.5

Adjustable

3

Price, U.S. $
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00

Preparative Combs*
1704442
1704441
1704440
1704328

4 preparative
2 reference
2 preparative
2 reference
1 preparative
2 reference
1 preparative
2 reference

Multichannel Pipet–Compatible Combs *170445626
Fixed
1704457
26
Fixed
170445418
Fixed170445518
Fixed
1704452
14
Fixed
1704453
14
Fixed
1704450
10
Fixed1704451
10
Fixed

0.75
1.5
0.75
1.5
0.75
1.5
0.75
1.5

27
3
50
4
106
4
106
4
3
3
3
3
6
6
6
6

14
14
20
19.1

15
15
14
14
20
20
14
14

202
22
375
30
795
30
1,590
60
11.25
22.5
11.25
22.5
22.5
45
22.5
45

57.00
57.00
57.00
57.00

57.00
57.00
57.00
57.00
57.00
57.00
57.00
57.00

* Fixed-height combs must be used with GT gel trays. Adjustable-height combs require comb holder, #1704320.
** Combs included in systems.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 259

Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems

See Also
Certified agaroses:
page 266.
Nucleic acidreagents: page 264.
PowerPac Basic andPowerPac HC power
supplies: page 185.
Premixed runningbuffers: page 265.

bio-rad.com/DNAelectro

ReadyAgarose Precast Gels
ReadyAgarose precast gels are prepared in gel trays
designed to fit securely in Mini-Sub Cell GT and wide
Mini-Sub Cell GT cells (page 255). They come in a choiceof 17 gel types, including ReadyAgarose 96 Plus gels,
which resolve DNA fragments from 20 to 10,000 bp.
Gels are individually packaged and cast in their own
running tray with Bio-Rad’s Certified agarose.
Gel types to choose from include:
■

■

■

■

■

■

Mini, wide, and 96-sample formats
1% and 3% agarose
TBE or TAE bufferWith or without ethidium bromide
Multichannel pipet–compatible wells
Compatible with Mini-Sub Cell GT and wide
Mini-Sub Cell GT cells

ReadyAgarose 96 Plus Products — Ideal for
High-Throughput Applications
ReadyAgarose 96 Plus products include:■■

ReadyAgarose 96 Plus precast gels
Wide mini ReadySub-Cell GT cell

For More Information
Web: bio-rad.com/agarosegel
Download bulletin: 2647

Ordering Information
Description

8-Well

12-Well

2 x 8-Well

Mini ReadyAgarose Gels, TBE
1.0% plus ethidium bromide

1613004

1613010—

11.00

Mini ReadyAgarose Gels, TAE
1.0%
1.0% plus ethidium bromide
3.0%161301516130161613017—16130221613057

11.00

20-Well

32-Well2 x 32-Well

161302816130301613034
1613036

—
—

15.00
22.00

16130441613050

1613054

15.00

Wide Mini ReadyAgarose Gels, TBE
1.0% plus ethidium bromide
3.0% plus ethidium bromide
Wide Mini ReadyAgarose Gel, TAE
1.0% plus ethidium bromide

Price, U.S. $11.00

4 x 26-Well
ReadyAgarose 96 Plus Gel, TBE
3.0% plus ethidium bromide

1613062

22.00

ReadyAgarose 96 Plus Gels, TAE
1.0% plus ethidium bromide
3.0% plus ethidium bromide

16130631613065

15.00
22.00

Catalog #Description

Price, U.S. $ReadySub-Cell GT Cells for ReadyAgarose Gels
1704487
Mini ReadySub-Cell GT Cell, includes buffer tank, lid and electrodes, leveling bubble;
accommodates 8-, 12-, and 2 x 8-well mini ReadyAgarose gels
1704489
Wide Mini ReadySub-Cell GT Cell, includes buffer tank, lid and electrodes, leveling bubble;
accommodates 20-, 32-, 2 x 32-, and 4 x 26-well wide mini ReadyAgarose gels

260 | Electrophoresis and Blotting

310.00455.00In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Electrophoresis and Blotting
bio-rad.com/PFGE

Pulsed Field Gel Electrophoresis

Pulsed Field Gel Electrophoresis
Pulsed field gel electrophoresis (PFGE) resolves large DNA molecules by alternating the electrical field between spatially
distinct pairs of electrodes, causing DNA molecules as large as several megabases to reorient and move at different
speeds through the pores in an agarose gel. Bio-Rad offers accessories for the clamped homogenous electrical field(CHEF) system that incorporates different PFGE technologies for optimal resolution in various size ranges.
Learn More about the Technology
Web: bio-rad.com/tech/pfge

Agaroses, Reagents, and Standards for PFGE
Bio-Rad offers a comprehensive line of agaroses (page 266), standards and markers (pages 262–263), buffers(page 265), and other reagents to make PFGE simple and convenient. See page 262 for genomic DNA plug
preparation kits.
For More Information
Web: bio-rad.com/PFGE

Ordering Information
Catalog #Description

Price, U.S. $Accessories for CHEF Mapper System*
1703644

Variable-Speed Pump, 120 V

1703648

Electrodes, thick gauge (0.02"), 6

1703711

Screened Caps, 5170371350-Well Disposable Plug Molds, enough for 250 plugs

1703622

Reusable Plug Mold, 10 plug

1703689

Standard Casting Stand, includes 14 x 13 cm frame and platform

1703643

Gel Scoop

1703699

Combination Comb Holder

Combs for CHEF Mapper System
1704326

10-Well Adjustable-Height Comb, 1.5 mm

1704325

10-Well Adjustable-Height Comb, 0.75 mm

1704324

15-Well Adjustable-Height Comb, 1.5 mm

1704323

15-Well Adjustable-Height Comb, 0.75 mm

1704322

20-Well Adjustable-Height Comb, 1.5 mm

170434430-Well Adjustable-Height Comb, 1.5 mm

1703627

15-Well Comb, 21 cm wide, 1.5 mm thick

170362830-Well Comb, 21 cm wide, 1.5 mm thick

1703645

45-Well Comb, 21 cm wide, 1.5 mm thick*A comprehensive listing of replacement parts can be found at bio-rad.com.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 261

Nucleic Acid Electrophoresis and Blotting
Pulsed Field Gel Electrophoresis

bio-rad.com/PFGECHEF Genomic DNA Plug Kits
CHEF genomic DNA plug kits provide a convenientmeans for preparing intact, chromosome-sized DNA
for PFGE. Three kits are available for the preparation ofbacterial (lysozyme-sensitive) or mammalian genomic
DNA and yeast chromosomes (YACs). Each kit contains
all the enzymes, reaction buffers, and restriction digestqualified CleanCut agarose necessary to prepare 100
plugs as well as disposable plug molds and screened
caps for simplified plug processing. Each kit is thoroughly
tested to ensure that prepared genomic DNA canbe restriction digested and separated on a CHEF
electrophoresis system.
Ordering Information
Catalog #Description

Price, U.S. $CHEF Genomic DNA Plug Kits
CHEF Mammalian Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml
1703591
proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, screened cap, 2 disposable
plug molds; makes 100 plugs
1703592
CHEF Bacterial Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml
proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, 1.6 ml lysozyme (25 mg/ml),
30 ml lysozyme buffer, screened cap, 2 disposable plug molds; makes 100 plugs
1703593
CHEF Yeast Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml
proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, 1.6 ml lyticase, 25 ml lyticase
buffer, screened cap, 2 disposable plug molds; makes 100 plugs
1703594
CleanCut Agarose, 2%, 12 ml

See Also
Pulsed field gelelectrophoresis
systems: page 261.
Imaging systems:
page 284.
Bioinformatics
software: page 289.
Certified agaroses:
page 266.

345.00455.00525.0030.00

Agaroses and Standards for Pulsed Field Gel Electrophoresis
Bio-Rad offers a comprehensive line of agaroses for use with PFGE as well as CHEF DNA standards and convenient
buffers and reagents to simplify your PFGE experiments. See page 266 for agaroses and page 263 for size standards
and buffers.

Pulsed Field Standards
Bio-Rad offers standards for applications from FIGE separation of cosmid inserts to the largest chromosomal separations.
The higher MW standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions.
DNA Ladders Selection GuideType

Description

Pulsed Field Standards
CHEF DNA standards

Derived from plasmids and lambda phage

CHEF DNA markers

Chromosomal DNA in low-melt agarose blocks

Pulsed Field Standards Selection Guide
		

Range

Contents

Amount

5 kb ladder
8–48 kb ladder
Lambda ladderS. cerevisiae
H. wingei

4.9–120 kb
8.3–48.5 kb
0.05–1 Mb
0.225–2.2 Mb
1–3.1 Mb

Concatemers of pBR328
Mixed digest of phage
Concatemers of phage cl857Sam7
Saccharomyces cerevisiae chromosomal DNA
Hansenula wingei chromosomal DNA

20 µg in 200 µl
25 µg in 125 µl
5 agarose blocks
5 agarose blocks
5 agarose blocks

262 | Electrophoresis and Blotting

Number of Applications
20–25
125
25–40
25–40
25–40

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Electrophoresis and Blotting
Pulsed Field Gel Electrophoresis

bio-rad.com/PFGE

Pulsed Field Standards
48.5 kb

2,200 kb
1,600

38.4

3.13 Mb

33.5

1,125
1,020
945

29.9
24.8

14.7 kb

825
785
750

19.4
15.0

9.8

680
610
565

10.0
8.3
145.5 kb
97
48.5
4.9
5 kb Ladder

8–48 kb Ladder

Lambda Ladder

450
365
285
225
S. cerevisiae
Chromosomal DNA

2.7
2.35
1.81
1.66
1.37
1.05
H. wingei
Chromosomal DNA

Ordering Information
Catalog #Description

Agaroses for Pulsed Field Gel Electrophoresis
1620137
Pulsed Field Certified Agarose, 100 g
1620138
Pulsed Field Certified Agarose, 500 g

Price, U.S. $235.001,045.00

Premixed Nucleic Acid Electrophoresis Buffers
1610733
10x Tris/Boric Acid/EDTA (TBE), 1 L
1610770
10x Tris/Boric Acid/EDTA (TBE), 5 L cube
1610743
50x Tris/Acetic Acid/EDTA (TAE), 1 L
1610773
50x Tris/Acetic Acid/EDTA (TAE), 5 L cube

37.00
110.0084.00
245.00

Pulsed Field Standards*
1703624
CHEF DNA Size Standard, 5 kb ladder, 4.9–120 kb, 20–25 applications
1703707
CHEF DNA Size Standard, lambda ladder, 8–48 kb, 125 applications
1703635
CHEF DNA Size Standard, lambda ladder, 0.05–1 Mb, 5 agarose blocks, sufficient for 25–40 plugs

205.0087.00
415.00

Pulsed Field Markers*
1703605
CHEF DNA Size Marker, S. cerevisiae, 0.2–2.2 Mb, 5 agarose blocks, sufficient for 25–40 plugs
1703667
CHEF DNA Size Marker, H. wingei, 1–3.1 Mb, 5 agarose blocks, sufficient for 25–40 plugs

150.00160.00* CHEF, clamped homogeneous electrical field. For more information, see page 261–262.

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 263

Nucleic Acid Electrophoresis and Blotting
Buffers and Reagents for Nucleic Acid Electrophoresis

bio-rad.com/nastains

Buffers and Reagents for Nucleic Acid Electrophoresis
Ethidium Bromide Solution
Ethidium bromide is a sensitive fluorescent stain forvisualizing DNA or RNA in agarose and polyacrylamidegels. Ethidium bromide is excited with a standard 302 nm
UV transilluminator and emits a red-orange signal that canbe photographed with Polaroid film or with a CCD-based
gel documentation system.
Bio-Rad’s premixed ethidium bromide solution eliminates
preparation steps and minimizes exposure to hazardous
ethidium bromide. Ethidium bromide solution is supplied
as a 10 mg/ml solution in 10 ml bottles.
For More Information
Web: bio-rad.com/nastains

DNA stained with ethidium
bromide.

Ordering Information
Catalog #Description

1610433

Ethidium Bromide Solution, 10 mg/ml, 10 ml

Price, U.S. $40.00

Tracking Dyes
Bio-Rad offers two tracking dyes to monitor
electrophoresis runs:■■

 romophenol blue for monitoring nucleic acid andBprotein electrophoresis
 ylene cyanole FF for monitoring nucleic acidX
electrophoresis
Ordering Information
Catalog #Description

1610404

Bromophenol Blue, 10 g

Price, U.S. $40.00

1610423

Xylene Cyanole FF, 25 g

40.00

Store at room temperature, dry, and away from direct sunlight.

264 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Electrophoresis and Blotting
Buffers and Reagents for Nucleic Acid Electrophoresis

bio-rad.com/dnareagents

See Also

UView 6x Loading Dye and Stain

UViewtransilluminator:
page 428.

Eliminate the need for gel staining with this easy-to-use
UView 6x loading dye. Because it also acts as an in-gel
stain, it saves precious time.
■

■

■

■

Loading dye plus in-gel stain
Saves time
Nontoxic
UV detection

For More Information
Web: bio-rad.com/uview

Ordering Information
Catalog #Description

16651111665112

UView 6x Loading Dye and Stain, 0.2 ml
UView 6x Loading Dye and Stain, 1 ml

Price, U.S. $
31.25
95.00

Premixed Running Buffers
Premixed running buffers can be used with handcast or
precast gels. Simply dilute with distilled deionized water.
Save time and standardize electrophoresis runs with thesepremixed running buffers.

Premixed Running Buffer Selection Guide
Buffer

1x Formulation

Applications

Nucleic Acid Electrophoresis
10x TBE		

89 mM Tris, 89 mM boric acid,
2 mM EDTA, pH 8.3

Nucleic acid electrophoresis/sequencing; polyacrylamide
or agarose gels

50x TAE		

40 mM Tris, 20 mM acetic acid,
1 mM EDTA, pH 8.0

Nucleic acid electrophoresis; polyacrylamide or agarose gels

Ordering Information
Catalog #Description

Premixed Nucleic Acid Electrophoresis Buffers
1610773
50x Tris/Acetic Acid/EDTA (TAE), 5 L cube
1610770
10x Tris/Boric Acid/EDTA (TBE), 5 L cube
1610743
50x Tris/Acetic Acid/EDTA (TAE), 1 L bottle
1610733
10x Tris/Acetic Acid/EDTA (TBE), 1 L bottle

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $245.00110.0084.00
37.00

Electrophoresis and Blotting | 265

Nucleic Acid Electrophoresis and Blotting
Buffers and Reagents for Nucleic Acid Electrophoresis

See Also
DNA electrophoresis
systems: page 254.
Overlay agaroses:
page 227.
CleanCut agarose:
page 262.
CHEF genomic
DNA plug kits:
page 262.
Premixed runningbuffers: page 265.

bio-rad.com/agarose

Certified Agaroses
All Certified agarose products are 100% pure and GQT
grade, guaranteeing the absence of inhibitors, DNases,
and RNases and minimizing background staining. Use theguide below to choose the agarose for your application.

Certified Agarose Selection Guide				
Application

Molecular				Biology
PCR
Low Range
Low-Melt
AgaroseAgaroseUltra Agarose
AgaroseAnalytical Separation≥1,000 bp≤1,000 bp
10–200 bp
1 kb–2 Mb
1 kb–5 Mb•

PCR		
Low-Melt
Megabase
AgaroseAgarosePulsedField
Agarose

•
•

•
•
•
•

Certified molecular biology agarose — this generalpurpose agarose ensures that DNA recovered from apreparative gel can be manipulated without compromisingquality. It has a very low sulfate content that yields a very
high gel strength and higher exclusion limit. The highelectrophoretic mobility increases resolution and reduces
run time, and the gels are easy to handle even at low
agarose percentages.
Certified PCR agarose — Certified PCR agarose isrecommended for separation of DNA fragments ≤1,000 bp.
This high-strength agarose forms gels that are easy tohandle even at high gel percentages, minimizing the riskof cracking or breaking. PCR agarose gels at 40°C so it isfaster and easier to prepare than GQT products with similar
sieving properties that gel at higher temperatures.
Certified low range ultra agarose — this agarose provides
superior resolution of small PCR fragments and primers.
A 3% gel clearly resolves a 10 bp ladder and a 4% gelapproaches the resolution of an 8% polyacrylamide gel.
Certified low-melt agarose — this low melting temperature
agarose has a high resolving capacity for DNA fragments≥1,000 bp. It is recommended for preparative electrophoresis•and for in-gel applications such as digestion and ligation.
It is also recommended for embedding chromosomes
and megabase-sized DNA for pulsed field applications.
Certified PCR low-melt agarose — this agarose yields
excellent resolution of fragments ≤1,000 bp in an analyticalor preparative format. It is ideal for digestion by agaroseand for all in-gel applications.
Certified megabase agarose — this Certified agaroseis the superior choice for CHEF and FIGE applications.
The gels are easy to handle even at concentrations as low
as 0.3%. The separation range is between 1 kb and 5 Mb.
Low background staining also provides superior imagingof high MW DNA.
Pulsed field Certified agarose — this agarose enables
excellent separation and resolution of large DNA fragmentsin pulsed field gel applications. The optimal separation
range is 1 kb–2 Mb. Running conditions for this agarose
are a preset selectable method of the CHEF Mapper XA
system auto-algorithm.
For More Information
Web: bio-rad.com/agarose
Download bulletin: 2755

Ordering Information
Description
Certified Agaroses for Standard Applications
Certified molecular biology agarose
Certified PCR agarose
Certified low range ultra agarose
Certified megabase agarose
Certified low-melt agarose
Certified PCR low-melt agarose
Catalog #Description

1620137
1620138

Pulsed Field Certified Agarose, 100 g
Pulsed Field Certified Agarose, 500 g

266 | Electrophoresis and Blotting

25 gPrice, U.S. $125 g

Price, U.S. $500 g

Price, U.S. $1613100
16131031613106
1613108
1613111161311361.00
140.00145.0089.00
170.00150.001613101—
16131071613109
16131121613114180.00350.00445.00295.00490.00435.001613102
1613105———
1613115580.001,475.00
930.001,630.00
Price, U.S. $235.001,045.00

In the U.S., call toll free at 1-800-424-6723

Nucleic Acid Electrophoresis and Blotting
DNA Ladders

bio-rad.com/dnaladders

DNA Ladders
Bio-Rad offers a broad variety of DNA ladders for conventional DNA gel electrophoresis, including molecular rulers
with evenly spaced banding patterns and EZ Load rulers premixed with loading buffer.

See Also

DNA Ladder Selection Guide

Micro test tubes:
page 423.

Type

Description

Molecular Rulers
Standard and EZ Load molecular rulers

DNA ladders of even base pair length increments, available in 5 size ranges

AmpliSize molecular ruler

Blunt-end DNA of precise length and known sequence

Pipet tips:
page 419.

ReadyAgarose
precast gels:page 260.
Imaging systems:
page 284.
DNA electrophoresis
systems: page 254.

Molecular Rulers Selection Guide						
Ruler
Concentration
Range
Number of Bands Reference Band

Amount,μg DNA

Suggested
Agarose Type

Number ofApplications

20 bp
EZ Load 20 bp

0.2 µg/µl
20–1,000 bp
0.1 µg/µl		

50 in 20 bp
200 bpincrements		

50

2.5–4%

100

100 bpEZ Load 100 bp

0.1 µg/µl
100–1,000 bp
0.05 µg/µl		

10 in 100 bp
None
increments		

25

2.5–4%

100

100 bp PCR
EZ Load 100 bp PCR

0.2 µg/µl
100–3,000 bp
0.08 µg/µl		

30 in 100 bp
increments

1,000 bp and
3,000 bp40

0.8–3%

100

500 bpEZ Load 500 bp

0.2 µg/µl
500–8,000 bp
0.08 µg/µl		

16 in 500 bp
increments

5,000 bp40

0.8–1%100

1 kb
EZ Load 1 kb

0.2 µg/µl
1–15 kb
0.08 µg/µl		

15 in 1 kb
increments

5 kb

40

0.8–1%

100

2.5 kb0.1 µg/µl
2.5–35 kb
				

14 in 2.5 kb
increments

10 kb

40

0.8%

100

10 of proprietary
blunt ended DNA

None

25

1.5–3%

50

0.1 µg/µl50–2,000 bp(10 ng/band/µl)		

AmpliSize		

Molecular Rulers
1,000 bp1,000 bp
900
800
700
600
500

15 kb

3,000 bp

400
300
200
180
160
140
120
100
80
60

200

2,000
1,000

35 kb

2,000 bp
1,500

10
8.0

1,000

6.0
5.0

700

4.0

500
400

10

300

3.0

1,500
1,000

200
2.0
100

		2.5

100

40
20
20 bp andEZ Load 20 bp
Rulers8,000 bp
7,000
6,000
5,000
4,000
3,500
3,000
2,500

100

50

500
1.0

100 bp and
EZ Load 100 bp
RulersIn the U.S., call toll free at 1-800-424-6723

100 bp PCR and
EZ Load 100 bp
PCR Rulers

500 bp and
EZ Load 500 bp
Rulers

1 kb andEZ Load 1 kb
Rulers

2.5 kb
Ruler

AmpliSize
Ruler

Electrophoresis and Blotting | 267

Nucleic Acid Electrophoresis and Blotting
DNA Ladders

bio-rad.com/dnaladders

Molecular Rulers
Molecular rulers are DNA ladders with precisely definedsize intervals between bands for simplified estimation of thelength of single- and double-stranded DNA separated on
agarose gels. Bio-Rad provides three types of molecularrulers for simplified estimation of length.

■

■

■

 tandard molecular rulers — DNA ladders of evenSbase pair length increments; ready for dilution
 Z Load molecular rulers — similar to standardE
molecular rulers but prediluted to a concentration
appropriate for most electrophoresis runs
 mpliSize molecular rulers — blunt-end DNA ofA
precise length and known sequence

Ordering Information
Catalog #Description

Price, U.S. $20 bp Molecular Rulers
1708201
20 bp Molecular Ruler, 250 µl, 20–1,000 bp, 100 applications
1708351
EZ Load 20 bp Molecular Ruler, 500 µl, 20–1,000 bp, includes 1 ml 5x nucleic acid sample buffer,
100 applications
100 bp Molecular Rulers
1708202
100 bp Molecular Ruler, 250 µl, 100–1,000 bp, 100 applications
1708352
EZ Load 100 bp Molecular Ruler, 500 µl, 100–1,000 bp, includes 1 ml 5x nucleic acid sample buffer,
100 applications
1708206
100 bp PCR Molecular Ruler, 200 µl, 100–3,000 bp, 100 applications
EZ Load 100 bp PCR Molecular Ruler, 500 µl, 100–3,000 bp, includes 1 ml 5x nucleic acid sample buffer,
1708353
100 applications
500 bp Molecular Rulers
1708203
500 bp Molecular Ruler, 200 µl, 500–8,000 bp, 100 applications
1708354
EZ Load 500 bp Molecular Ruler, 500 µl, 500–8,000 bp, includes 1 ml 5x nucleic acid sample buffer,
100 applications

115.00115.00115.00115.00115.00115.00115.00115.00

1 kb Molecular Rulers
1708204
1 kb Molecular Ruler, 200 µl, 1–15 kb, 100 applications
1708355
EZ Load 1 kb Molecular Ruler, 500 µl, 1–15 kb, includes 1 ml 5x nucleic acid sample buffer, 100 applications

93.00
93.00

2.5 kb Molecular Ruler
1708205
2.5 kb Molecular Ruler, 400 µl, 2.5–35 kb, 100 applications

165.00

AmpliSize Molecular Ruler
1708200
AmpliSize Molecular Ruler, 250 µl, 50–2,000 bp, 50 applications

165.00268 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting
Subject Index

Subject Index
2-D Premixed Buffers and Individual Reagents, 227

PowerPac Universal Power Supply, 186

2-D SDS-PAGE Standards, 191

Precision Plus Protein Standards, 188

Agaroses and Standards for Pulsed Field Gel Electrophoresis, 262

Premixed Blotting Buffers and Buffer Reagents, 245

Bio-Dot and Bio-Dot SF Microfiltration Apparatus, 240

Premixed Gel-Casting Buffers, 215

Bio-Lyte Ampholytes, 220

Premixed Running Buffers, 215, 265

Blotting-Grade Conjugates and Reagents, 252

Premixed Sample Loading Buffers, 214

Blotting Stains and Tracking Dyes, 244

PROTEAN i12 IEF Accessories, 224

Bromophenol Blue Tracking Dye, 219

PROTEAN II Multi-Gel Casting Chamber, 211

Certified Agaroses, 266

PROTEAN II xi and XL Accessories, 209

CHEF Genomic DNA Plug Kits, 262

PVDF Membranes, 243

Clarity and Clarity Max Western ECL Substrates, 248

ReadyAgarose Precast Gels, 260

Cleaning Concentrate, 220

ReadyPrep 2-D Starter Kit, 226

Colorimetric AP Detection, 251

ReadyStrip IPG Strips, 225

Colorimetric HRP Detection, 250

Silver Stains, 222

Combs for Wide Mini-Sub Cell and Sub-Cell GT Cells, 259

Sub-Cell GT Cell, 258

Coomassie Stains, 221

TGX Handcast Acrylamide Solutions, 216

Criterion Accessories, 208

Total Protein Stains, 253

Criterion Blotter, 238

Tracking Dyes, 264

Criterion Cell and Criterion Dodeca Cell, 201

Trans-Blot SD Semi-Dry Transfer Cell, 236

Criterion Precast Gels, 202

Trans-Blot Turbo Transfer System, 235

Crosslinkers and Catalysts, 218

UView 6x Loading Dye and Stain, 265

Detergents, 219

Wide Mini-Sub Cell GT Cells, 257

Ethidium Bromide Solution, 264
EveryBlot Blocking Buffer, 245
Filter Paper, 244
Fluorescent Stains, 223
Gel-Casting Reagents, 217
Gel Drying Solution, 220
IEF Standards, 191
IEP/IEF Agaroses, 218
Immun-Star AP Chemiluminescence Kits, 249
Large-Format Electrophoresis Accessories, 212
Mini Incubation Trays, 241
Mini-PROTEAN 3 Dodeca Cell, 195
Mini-PROTEAN Hand Casting Accessories, 199
Mini-PROTEAN II Multiscreen Apparatus, 241
Mini-PROTEAN Precast Gels, 196
Mini-PROTEAN Tetra Cell, 192
Mini-Sub Cell GT Cells, 255
Mini Trans-Blot Cell, 237
Model 111 Mini IEF Cell, 228
Model 422 Electro-Eluter, 231
Model 491 Prep Cell and Mini Prep Cell, 229
Molecular Rulers, 268
Nitrocellulose Membranes, 242
PowerPac Adaptor, 186
PowerPac Basic Power Supply, 185
PowerPac HC High-Current Power Supply, 185
PowerPac HV High-Voltage Power Supply, 185

In the U.S., call toll free at 1-800-424-6723

Electrophoresis and Blotting | 269

270 | Electrophoresis and Blotting

In the U.S., call toll free at 1-800-424-6723

Antibodies
Antibodies

272

Antibodies
bio-rad-antibodies.com

Antibodies

Thousands of antibodies, reagents, standards, and kitsavailable for research use. Specificity and performance arehallmarks of the antibody collections. Antibodies can beused for many applications, including:
■

■

■

■

■

Western blotting
Flow cytometry
Immunohistochemistry
Enzyme-linked immunosorbent assay
Immunofluorescence

Antibodies are available for the following research areas:
CancerCell biology
Immunology
Neuroscience
Regulated cell death
Stem cell research
Veterinary immunology
■

■

104
CD3 A488

Bio-Rad offers an extensive selection of antibodies on our
dedicated antibody website, bio-rad-antibodies.com.
Antibodies can now also be added to your cart on
bio-rad.com.

105

103

102

101

10 0
10 0

101

102
103
CD69 A647

104

105

Flow cytometry analysis of activated human T cells. Humanperipheral blood lymphocytes were stimulated with phytohemagglutinin
and lipopolysaccharide for 48 hours prior to being dual stainedwith mouse anti-human CD3A488 (#MCA463A488) and CD69A647(#MCA2806A647) in the presence of human seroblock (#BUF070A).
Forward and side scatter gating was applied. Activated T cells can be
identified as positive for both CD3 and CD69.

■

■

■

■

■

Antibodies conjugated to over 20 dyes, including Bio-Rad's
StarBright Dyes, Alexa Fluor, DyLight, fluorescein
isothiocyanate (FITC), and phycoerythrin (PE), for multicolor
flow cytometry are also available.
In addition, Bio-Rad offers the PrecisionAb antibody
product line, which is extensively validated for western
blotting (see page 273).
Antibodies are highly specific and come with aperformance guarantee. The following data show two of
the many antibody clones that are unique to Bio-Rad.
Bio-Rad offers custom services for the in vitro generation of
fully human monoclonal antibodies. Specific Fab antibodies
can be generated within 8 weeks, with a greater than 90%
success rate and without the immunization of animals.
Antibodies can be customized to specific needs.
Bio-Rad has developed an entirely new phage display
library called Pioneer Antibody Library. Harnessing SpyTagtechnology, it has been engineered with a proprietary
display method, SpyDisplay, to enable the rapid discoveryof therapeutic antibody candidates. The Pioneer Antibody
Library is an exceptionally large functional phage display Fabantibody library, containing 2.15 x 1011 unique antibodies,
thereby delivering a high and diverse number of unique hits.

Immunoperoxidase staining of anti-rat CD68, a macrophage
marker. A rat lymph node cryosection was probed with mouseanti-rat CD68 (#MCA341GA) antibody, clone ED1, followed byhorseradish peroxidase–conjugated goat anti-mouse IgG1 antibody.

The large size of the library ensures very high affinities
from initial pannings increasing the probability oftherapeutic candidate antibodies from the outset and with
sub-nanomolar affinities possible from the initial pannings.
Affinity maturation can be used to further optimize
promising therapeutic candidates.
The HuCAL PLATINUM® library contains more than
45 billion functional human antibody specificities
already prefabricated in an engineered Fab format. The
technology uses CysDisplay®, a novel and proprietary
phage display method that allows for the selection ofhigh-affinity antibodies that are expressed on the surface
of a filamentous phage. SpyTag-SpyCatcher technology
provides you with an extensive antibody toolbox, allowing
an antibody to be supplied in a variety of formats.
For More Information
Web: bio-rad-antibodies.com

272 | Antibodies

In the U.S., call toll free at 1-800-424-6723

Antibodies
bio-rad.com/precisionab

PrecisionAb Primary Antibodies Validated for Western Blotting

■

■

■

■

High specificity — each antibody is extensively
validated to ensure that it exhibits low nonspecific binding igh sensitivity — all antibodies must be able to detectH
the protein of interest at endogenous levels
 eproducible performance — stringent QC proceduresR
ensure consistent lot-to-lot performance

For More Information
Web: bio-rad.com/precisionab

t
Raji

Jurka

-7
MCF

50
37
25
20
15
10

 xpanding selection of antibodies – in cancer,E
neuroscience, and immunology esting against cells and tissues expressing theT
endogenous target

HeL a

150
100
75

KO

■

250

WT

Product features include:

H EK 2

Methods for Antibody Validation
These include genetic strategies such as measuring therelevant signal in control cell lines, where the target genehas been knocked out by CRISPR-Cas9.

93

Primary Antibodies for Reproducible Western Blots
PrecisionAb antibodies are rigorously validated for western
blotting. Each antibody is screened using Bio-Rad’s StainFree western blotting workflow. PrecisionAb antibodies area premium line selected to deliver industry-leading quality
and performance.

250
150
100
75

Lipoma-PreferredPartner

50
37

GAPDH

25
20
15
10

To assess antibody performance, we analyze multiple cell lines
and tissues known to express the target, as well as negative
controls. The results are published as images on our website and on
the antibody datasheets.

In the U.S., call toll free at 1-800-424-6723

Antibodies | 273

Antibodies
bio-rad.com/phospho-precisionab

Phospho-Specific PrecisionAb Antibodies

To showcase the different binding characteristics ofphospho-specific and total protein antibodies, we havecompared phospho-specific PrecisionAb antibodies
against total PrecisionAb antibodies. This enables you toassess the impact of the different treatments and provides
a control recommendation. The total and phospho-specificantibodies can be purchased individually.

D

D

+ PV
A431

A431

kDa

250
150
100
75
50
37
25
20
15
10
l phosphatase – –

+ PV
A431

A431

A431

kDa

A431

A
B

+ PV

+ PV
A431

A

Mouse anti EGF R(pTyr845) antibody

D

D

Mouse anti EGF R(total protein) antibody

A431

Bio-Rad’s phospho-specific PrecisionAb antibodies
have been validated by comparing phosphorylationlevels in both treated and untreated cell lysates and bydephosphorylating proteins on western blot membranes,
as seen here. The phosphorylation level quantification
enables you to quickly determine the impact of cellulartreatments and provides you with an easy way to assess
the antibody specificity.

EGF R

+ +

250
150
100
75
50
37

25
20
15
10
l phosphatase – –EGF R(pTyr845)+ +For More Information
Web: bio-rad.com/phospho-precisionab

Ordering Information
Catalog #Description

VMA00746
VMA00749
VMA00750
VMA00751
VMA00752
VMA00748
VMA00753
VMA00754
VMA00755
AHP2604
AHP2605
AHP905
AHP2666

Mouse Anti-EGF Receptor (pSer1047)
Mouse Anti-EGF Receptor (pTyr1068)
Mouse Anti-EGF Receptor (pTyr1086)
Mouse Anti-EGF Receptor (pTyr1148)
Mouse Anti-EGF Receptor (pTyr1173)
Mouse Anti-EGF Receptor (pTyr845)
Mouse Anti-ErbB2 (pTyr1112)
Mouse Anti-GSK3 Alpha (pSer21)
Mouse Anti-STAT3 (pSer727)
Rabbit Anti-ErbB3 (pTyr1328)
Rabbit Anti-ErbB4 (pTyr1284)
Rabbit Anti-p38 MAPK (pThr180/pTyr182)
Rabbit Anti-p53 (pSer6)

274 | Antibodies

Price, U.S. $44.00
300.0053.00
300.00In the U.S., call toll free at 1-800-424-6723

Antibodies
bio-rad.com/cancer

Rabbit Monoclonal Antibodies for Cancer Research
Bio-Rad offers a premium range of recombinant rabbit
monoclonal antibodies against targets widely investigatedin cancer research. These include proteins involved in the
AKT, EGF R, and NFKB signaling pathways.

Immunocytochemical staining of
HeLa cells, using Rabbit
Anti-Paxillin (MCA6310 — red).
Actin filaments have been labeledwith fluorescein phalloidin (green) andthe nucleus labeled with DAPI (blue).

Our quality assured Rabbit Monoclonal Antibodies have been:Tested for use in a wide range of applications including
WB, IHC, and IF
■

■

■

■

 elected using a stringent process to ensure highSaffinity binding urified and recombinantly produced from an animalP
origin-free source

For More Information and Full List of Antibodies Available
Web: bio-rad.com/rabbitmono

 ecombinant technology ensures lot-to-lot consistencyRand a long-term secure supply of your antibodies.

Catalog Bioanalytical Antibodies
Bio-Rad offers over a hundred antibodies against marketedbiologic drugs, such as abtacept, infliximab, and rituximab,that are suitable for pharmacokinetic (PK) and anti-drug–
antibody (ADA) assays to support both bioanalytical assays

for preclinical research and clinical trials and therapeutic
drug monitoring for innovator and biosimilar products.
For More Information
Web: bio-rad.com/anti-ids

Antibodies for Epigenetic Research
Epigenetics investigates heritable changes in gene
expression without changes to the DNA sequence.
Bio-Rad's comprehensive antibody portfolio for epigenetic
research can be used to study epigenetic changes,
chromatin modifying proteins, and transcription factors
in various applications including western blotting, ChIP,
and immunofluorescence. Browse Bio-Rad’s range ofantibodies against epigenetics factors.
For More Information
Web: bio-rad-antibodies.com/epigenetic

Rabbit anti DNA Methyltransferase 1 antibody
DNA Methyltransferase 1 KO
WT
kDa
250
150

DNA Methyltransferase 1

100
75
50

Tubulin

37
25
20
15
10

Western blot analysis of DNA methyltransferase 1 knockout 293T
(KO) and wild type (WT) whole cell lysates probed with Rabbit
Anti-DNA Methyltransferase 1 Antibody (VPA00026).

In the U.S., call toll free at 1-800-424-6723

Antibodies | 275

Antibodies
bio-rad.com/starbrightblue

StarBright Fluorescent Secondary Antibodies
StarBright Blue secondary antibodies are labeledwith exceptionally bright and stable semiconducting
nanoparticles, which provide an exceptionally bright signal
compared to most traditional fluorophores.
StarBright Blue 700 (excitation/emission = 470/700 nm)
and StarBright Blue 520 (excitation/emission = 488/520)
are Bio-Rad’s ultrasensitive fluorescent labels that allow
detection of low-abundance proteins within seconds of
exposure time with minimal background. Highly crossadsorbed goat anti-rabbit and goat anti-mouse secondary
antibodies conjugated to StarBright Dyes are ideal for
fluorescent western blotting, either for the detection of asingle target protein or for multiplex detection of several
proteins on one blot without stripping and reprobing. These
antibodies are optimized for use with the ChemiDoc MP
imaging system, permitting detection of multiple proteins ina single blot, thus saving time, sample, and reagents.
■

■

■

■

■

 hort exposure time — exceptional brightness of
S
the StarBright Dye leads to shorter exposure times
compared to traditional fluorescent dyes:
seconds vs. minutes
 igh signal-to-noise ratio — StarBright Blue 700
Hsecondary antibodies emit in the far red part of thespectrum where there is minimal background (excitation
maximum/emission maximum = 470/700 nm)
 reater sensitivity — StarBright Blue 520 is 2- to 4-foldG
more sensitive than other green-emitting fluorophores,
such as Alexa Fluor 488 or DyLight 488
 ow nonspecific binding — StarBright Dyes areLconjugated to highly cross-adsorbed antibodies

For More Information
Web: bio-rad.com/starbrightblue
Request or download bulletin: 6911

 asy multiplexing — StarBright secondary antibodies
E
can be used with IRDye 800CW or other traditional red,green, and blue (RGB) fluorophore to detect multiple
proteins on the same blot. They can also be used with
Bio-Rad’s Stain-Free technology and/or hFAB rhodamine
housekeeping protein fluorescent primary antibodies
for protein normalization

Ordering Information
Catalog #Description

12004159
12004158
12004162
12004161
12005867
12005866
12005870
12005869

StarBright Blue 700 Goat Anti-Mouse IgG, 80 µl
StarBright Blue 700 Goat Anti-Mouse IgG, 400 µl
StarBright Blue 700 Goat Anti-Rabbit IgG, 80 µl
StarBright Blue 700 Goat Anti-Rabbit IgG, 400 µl
StarBright Blue 520 Goat Anti-Mouse IgG, 80 µl
StarBright Blue 520 Goat Anti-Mouse IgG, 400 µl
StarBright Blue 520 Goat Anti-Rabbit IgG, 80 µl
StarBright Blue 520 Goat Anti-Rabbit IgG, 400 µl

276 | Antibodies

Price, U.S. $44.00
300.0053.00

In the U.S., call toll free at 1-800-424-6723

Antibodies
bio-rad.com/hkp

hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies
hFAB rhodamine fluorescent primary antibodies aremonovalent human Fabs, which allow one-step detectionof common housekeeping proteins actin, tubulin,
and GAPDH in human, mouse, or other common
mammalian samples. These anti-actin, anti-tubulin, andanti-GAPDH antibodies are directly labeled with arhodamine derivative (excitation maximum/emission
maximum = 530/580 nm). The hFAB rhodamine antibodies
were generated with Human Combinatorial Antibody
Library (HuCAL® ) technology, making these recombinant
antibodies ideal for use in multiplex western blotting
without worrying about species cross-reactivity.
These housekeeping antibodies can be used for protein
normalization when the loading amounts are in the linear
dynamic range. They can be used in multiplex experimentswith StarBright Blue 700 fluorescent secondary antibodies
as well as other traditional fluorescent antibodies, permitting
detection of multiple target proteins and the housekeeping
protein in a single blot.
■■

 imple — no requirement for a secondary antibody;S
1-step detection of housekeeping proteins using highly
cross-adsorbed antibodies for high specificity andsensitivity■■

Unique — HuCAL technology eliminates cross-reactivity
with primary or secondary antibodies from other speciesIdeal for multiplexing — allows simultaneous detectionof proteins of interest and housekeeping proteins on thesame blot without stripping, reprobing, or cutting themembraneFc

FabRhodamine-labeledmonovalent hFAB

Schematic representation comparing the structure of the
IgG molecule with the rhodamine-labeled monovalent hFAB
housekeeping antibody.
For More Information
Web: bio-rad.com/hkp
Request or download bulletin: 6911

Versatile — compatible with a wide range of otherfluorophores, including StarBright Blue 700 and
DyLight 800 fluorophores, for multiplex western blotting

Ordering Information
Catalog #Description

12004164hFAB Rhodamine Anti-Actin Primary Antibody, 40 µl, pkg of 1, lyophilized rhodamine-conjugated antiactin human Fab fragment; reconstitute to 40 µl
hFAB Rhodamine Anti-Actin Primary Antibody, 200 µl, pkg of 1, lyophilized rhodamine-conjugated antiactin human Fab fragment; reconstitute to 200 µl
hFAB Rhodamine Anti-Tubulin Primary Antibody, 40 µl, pkg of 1, lyophilized rhodamine-conjugated antitubulin human Fab fragment; reconstitute to 40 µl
hFAB Rhodamine Anti-Tubulin Primary Antibody, 200 µl, pkg of 1, lyophilized rhodamine-conjugated antitubulin human Fab fragment; reconstitute to 200 µl
hFAB Rhodamine Anti-GAPDH Primary Antibody, 40 µl, pkg of 1, lyophilized rhodamine-conjugated antiGAPDH human Fab fragment; reconstitute to 40 µl
hFAB Rhodamine Anti-GAPDH Primary Antibody, 200 µl, pkg of 1, lyophilized rhodamine-conjugated antiGAPDH human Fab fragment; reconstitute to 200 µl

12004163
12004166
12004165
12004168
12004167

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $44.00
300.0053.00
300.00

Antibodies | 277

Antibodies
bio-rad.com/isotype-secondaries

Secondary Antibodies

The following products are available on
bio-rad-antibodies.com.
Secondary antibodies are commonly used to detect andvisualize the presence of a primary antibody in applications
including western blotting or immunofluorescent histology.
In these applications, the secondary antibody is labeledwith a reporter molecule, which may be an enzyme suchas HRP or a fluorophore such as FITC. Multiple secondary
antibodies can bind to a single primary antibody, increasing
the sensitivity and amplifying the signal.
Bio-Rad's secondary reagents have been carefully
selected to provide optimum quality and flexibility for yourexperimental design. They are suitable for a wide range ofapplications including flow cytometry, western blotting, andimmunocytochemistry. Options include:■

Monoclonal or polyclonal■

IgG molecules or F(ab')2 fragments■

Multiple formats (see product options below)■

Cross-adsorbed and isotype-specific antibodies■

Fluorescent staining of human colon using secondary antibodies.
FFPE human colon adenocarcinoma stained with mouse anticytokeratin 18 human anti-mouse IgG1 (#HCA309D488) (green),mouse anti-PCNA (#MCA1558), and human anti-mouse IgG2a(#HCA310D550) (red).
For More Information
Web: bio-rad.com/secondaries

 luorescent StarBright secondary antibodies
F(exceptionally bright and ideal for multiplex
western blotting)

Secondary antibody product options.
TargetHostClass and Chain

Conjugates

Human
Mouse
Rat
Goat
RabbitSheep
BovineGuinea pig
Cat
ChickenMonkeyFish
Dog
Hamster
Horse
Pig

DonkeyRabbit
Goat
Mouse
Sheep
Rat
Human

Ig
IgA, IgA A1, IgA A2, IgA H, IgA secretory chain
IgD, IgD H, IgD D
IgE
IgG, IgG1, IgG2, IgG2a, IgG2b, IgG2c, IgG3
IgG4, IgG H/L, IgG Fc, IgG Fc CH2 domain, IgG
CH2 domain, IgG CH3 domain, IgG gamma, IgG
Fab, IgG F(ab')2
IgM, IgM Mu, IgM H
IgG/A/M
Lambda light chain
Kappa light chain
J chain

Purified
Serum
S/N
FITC
Tritc
HRP
BiotinAlkaline phosphatase
Texas RedStarBright Blue
DyLight 405
DyLight 488
DyLight 550
DyLight 650
DyLight 680
DyLight 800
Bgal

278 | Antibodies

In the U.S., call toll free at 1-800-424-6723

Antibodies
bio-rad.com/isotype-secondaries

Isotype Specific Secondary Antibodies
Recombinant monoclonal antibodies directed against thethree main mouse isotypes, IgG1, IgG2a, and IgG2b, arean alternative to cross-adsorbed secondary antibodies.
They are capable of detecting individual isotypes without
any species or isotype cross-reactivity, enabling multiple
unlabeled mouse monoclonal antibodies to be usedsimultaneously. Multiplexing is therefore straightforward
and an alternative species does not need to be sourced.
Benefits of isotype-specific secondary antibodies:High specificity with reduced backgroundNo isotype cross-reactivity
Higher signal level
Ideal for multiplexing
Easy to use
Fewer extra steps, saving time
■

■

■

■

■

■

For More Information
Web: bio-rad.com/isotype-secondaries

Ordering Information
Catalog #Description

HCA309
HCA310
HCA311

Isotype Specific Secondary Antibodies, human anti-mouse IgG1
Isotype Specific Secondary Antibodies, human anti-mouse IgG2a
Isotype Specific Secondary Antibodies, human anti-mouse IgG2b

Price, U.S. $44.00
300.00

New Pioneer Antibody Discovery Platform
Bio-Rad has developed Pioneer Library, a new Antibody
Discovery Platform comprising an entirely new phagedisplay library. Harnessing SpyTag technology, it has
been engineered with a proprietary display method,
SpyDisplay, to enable the rapid discovery of therapeutic
antibody candidates. The Pioneer Antibody Library is an
exceptionally large functional phage display Fab antibody
library, containing 2.25 x 1011 unique antibodies, thereby
delivering a high and diverse number of unique hits.

In the U.S., call toll free at 1-800-424-6723

The large size of the library ensures very high affinities frominitial pannings increasing the probability of therapeuticcandidate antibodies from the outset and with subnanomolar affinities possible from the initial pannings.
Affinity maturation can be used to further optimize
promising therapeutic candidates.
For More Information
Web: bio-rad-antibodies.com/pioneer

Antibodies | 279

Antibodies
bio-rad.com/tidyblot

TidyBlot Western Blot Detection Reagent:HRP
TidyBlot reagent (STAR209P, STAR209PA, andSTAR209PT) specifically binds to native antibodies. In
contrast to conventional secondary antibodies, TidyBlot
reagent enables the detection of immunoblotted target
protein bands without interference from denatured IgG.
Sources of denatured IgG include endogenous IgGs
present in cell/tissue lysate and primary antibodies

released from beads during immunoprecipitation
experiments. The latter are particularly obstructive when
detecting (co-) immunoprecipitated proteins, as IgG
heavy (~50 kDa) and light (~25 kDa) chains may mask theprotein(s) of interest.
For More Information
Web: bio-rad.com/tidyblot
kDa

Goat anti
Mouse lgG (H/L)(STAR207P)

kDa
250

1

2

3

4

5

6

150
100
75
TidyBlot(STAR209P)

50
37

150
100
75

25

50

20

Actin gamma

50 kDa Heavy chain
46 kDa actin gamma

25 kDa Light chain

37
25
20
15

15

Light chain

10
1

2

3

4

5

6

Comparison of actin gamma detection in mouse thymus
lysate using goat anti-mouse IgG (H/L) or TidyBlot reagent as
secondary detection reagents. Precision Plus Protein prestained
standards were run in lanes 1 and 4. Mouse anti-actin gamma
antibody was used in lanes 2 and 5. Lanes 3 and 6 are secondaryonly controls. HRP conjugated goat anti-mouse IgG (H/L) secondary
antibody (#STAR207P) was used in lanes 2 and 3. TidyBlot western
blot detection reagent:HRP (#STAR209P) was used as the secondary
reagent in lanes 5 and 6. Please note that TidyBlot reagent is
HRP conjugated and that the western blot bands (red) and protein
standards (green) have been pseudocolored.

Comparison of HRP conjugated TidyBlot western blot
detection reagent (lanes 2 and 3) to a standard anti-mouse
IgG (H/L) secondary antibody (lanes 5 and 6). In contrast to theanti-mouse secondary antibody, TidyBlot reagent binds only to themouse anti-human actin gamma antibody used for detection (lane2). Lanes 3 and 6 are no-primary antibody controls. Please note that
TidyBlot reagent is HRP conjugated and that the western blot bands(red) and protein standards (green) have been pseudocolored.

Ordering Information
Catalog #Description

STAR209PT
STAR209P
STAR209PA

TidyBlot Western Blot Detection Reagent:HRP, 50 µl
TidyBlot Western Blot Detection Reagent:HRP, 0.5 ml
TidyBlot Western Blot Detection Reagent:HRP, 1 ml

280 | Antibodies

Price, U.S. $44.00
300.00In the U.S., call toll free at 1-800-424-6723

Antibodies
bio-rad.com/starbright

StarBright Dyes for Flow Cytometry
StarBright Dyes are unique, proprietary, fluorescent
nanoparticles conjugated to Bio-Rad’s highly validated
antibodies. They have been specially developed for flow
cytometry to give exceptional brightness and are for use inmulticolor panels in immunophenotyping. The brightness
allows easy resolution of rare populations and low-density
antigens. In addition, these dyes have the flexibility to fit into
any multicolor panel without the need for special buffers.

Essential features of StarBright Dyes:
BrightCompatible with all buffers
Narrow excitation and emission
StableLot-to-lot reproducibility
Suitable for conventional and spectral flow cytometry andcell sorting experiments
■

■

■

■

■

■

New dyes will be added to the StarBright range throughout
the year.
For More Information
Web: bio-rad.com/starbright
Bright

Lot-to-Lot Consistency

595

105

80

298

149

104
CD8 SBV710

Normalized To Mode

447

Count

Multiplexing

100

60

40

103

102

20

-101
-102
0

0
10 1

10 2
10 3
355: 387/11

10 4

10 5

10 0

10 5

-102 -10 0 102

Lot-to-Lot Consistency

105

105

104

104
355: 508/25

CD4 SBV670

Bright

10 1
10 2
10 3
10 4
Como-FL06-A: SBB700-A

103

102

103
104
CD4 BV610

105

Multiplexing
10 6

105
CD8 SBV610

10 0

103

102

104

103
-

103

101

101

10 0

10 0
Unstained

PBS+
1% BSA

Bio-Rad
Stain
Buffer

Brilliant
Stain
BufferIn the U.S., call toll free at 1-800-424-6723

Unstained Fresh

Bio-RadFixation
Buffer2%
PFA

Ethanol

-104

-103

104
105
CD4 BV605

10 6

Antibodies | 281

Antibodies
Subject Index

Subject Index
PrecisionAb Primary Antibodies Validated for Western Blotting, 273
Phospho-Specific PrecisionAb Antibodies, 274
Rabbit Monoclonal Antibodies for Cancer Research, 275
Catalog Bioanalytical Antibodies, 275
Antibodies for Epigenetic Research, 275
StarBright Fluorescent Secondary Antibodies, 276
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies, 277
Secondary Antibodies, 278
Isotype Specific Antibodies, 279
Pioneer Library Therapeutic Antibody Discovery, 279
TidyBlot Western Blot Detection Reagent:HRP, 280
StarBright Dyes for Flow Cytometry, 281

282 | Antibodies

In the U.S., call toll free at 1-800-424-6723

Imaging Instruments,
Analysis, and Software
Imaging Systems

284

Blot and Gel Image Analysis Software289

Imaging Systems
bio-rad.com/imaging

Imaging Systems
Imaging systems detect images and quantitate fluorescent, chemiluminescent, and colorimetric signals. Bio-Rad offers
software (page 289) that provides automation for image acquisition with data analysis and validation. Refer to the guide
below to select the imaging system best suited for your applications.
Learn More about the Technology
Web: bio-rad.com/tech/proteinimaging
Imaging System Selection Guide

Application

ChemiDoc MP

ChemiDoc

ChemiDoc XRS+

GelDoc Go

GS-900Blot Detection
Multiplex fluorescence

✓

—

—

—

—

Chemiluminescence

✓

✓

✓

—

—

Stain-Free

✓

✓

✓

✓

—

Colorimetric

✓

✓

✓

✓

—

Ethidium bromide stain

✓

✓

✓

✓

—

SYBR® Green and
SYBR® Safe stains

✓

✓

✓

✓

—

Fast Blast DNA stain

✓

✓

✓

✓

✓

Nucleic Acid Detection

Protein Detection, 1-D Gels
Stain-Free gels

✓

✓

✓

✓

—

Coomassie Blue stain

✓

✓

✓

✓

✓

Silver stain

✓

✓

✓

✓

✓

Oriole fluorescent gel stains

✓

✓

✓

✓

—

Coomassie Blue stain

✓

✓

✓

✓

✓

Silver stain

✓

✓

✓

✓

✓

Flamingo and Oriole fluorescent
gel stains

✓

✓

✓

✓

—

Cy2, Cy3, Cy5 label

✓

—

—

—

—

Protein Detection, 2-D Gels

✓ Recommended; — not recommended.

284 | Imaging Instruments, Analysis, and Software

In the U.S., call toll free at 1-800-424-6723

Imaging Systems
bio-rad.com/imaging

See Also

ChemiDoc MP and ChemiDoc Imaging Systems

Stain-Free gels(mini, midi formats):
pages 196, 203.

The ChemiDoc MP and ChemiDoc imaging systems are
Bio-Rad’s premium digital imaging systems.

Stain-Free western
blotting: page 232.

The ChemiDoc MP imaging system provides the best inchemiluminescent and fluorescent blot detection. It
excels at multiplex fluorescent (red, green, and blue
[RGB] and near infrared/infrared) western blotting,chemiluminescence detection, and general gel
documentation applications. Simply pick the best
detection method for your application. The ChemiDoc
imaging system is built to allow ultrasensitive detectionof chemiluminescent signal with the option to upgrade to
ChemiDoc MP to enable fluorescence detection.

Trans-Blot Turbotransfer system:
page 235.

Videos
Product
Spotlight

Both systems include Bio-Rad’s Image Lab Touch software.
Turn on the instrument and tap, explore, and learn inminutes, skipping lengthy instruction manuals. The software
includes advanced user accounts and administrative
features for better data security and integrity.

bio-rad.com/
CDexperience

Product
Demonstration

With the ChemiDoc MP and ChemiDoc imaging systems
you can:■■ et more reliable, trustworthy, and publishable dataGusing Stain-Free technology to normalize your resultswith more than just a single protein
 btain the next level of fluorescence sensitivity withO
StarBright Blue 700 fluorescent secondary antibodies,
which are more sensitive than traditional far red and nearinfrared dyes

For More Information
Web: bio-rad.com/chemidoc
Request or download bulletin: 6873

bio-rad.com/
CDMPchemi

Customer
Testimonial
Blue

Green

Red

StarBrightBlue 520

Far RedNear
Infrared

StarBrightBlue 700

DyLight 488

DyLight 550

DyLight 650

DyLight 680

DyLight 800

Alexa Fluor
488

Alexa Fluor
546

Alexa Fluor
647

Alexa Fluor
680

Alexa Fluor
790

Cy2

Cy3

Cy5

Cy5.5

Cy7

Rhodamine

SYPRO
Ruby

IRDye 680

IRDye
800CW

bio-rad.com/
CDdavisCustomer
Testimonial

Flexible dye selection with the ChemiDoc MP imaging system.
bio-rad.com/
CDkorea

Ordering Information
Catalog #Description

12003154
12003153

ChemiDoc MP Imaging System, ultrasensitive chemiluminescence and fluorescence western blot imaging system
ChemiDoc Imaging System, ultrasensitive chemiluminescence western blot imaging system with optional upgrade
for fluorescence detection
ChemiDoc MP and ChemiDoc IQ/OQ Kit, installation and operational qualification kit, includes protocols andimaging target
White Sample Tray, for white light applications
Blue Sample Tray, for low-intensity ultraviolet and SYBR applications
Blot/UV/Stain-Free Sample Tray, primary blot and gel detection tray, replacement for tray included with system
Holder for Sample Trays and UV Shield, pkg of 1, holds 3 sample trays and 1 UV shield; trays and shield not included
UV Safety Shield, to protect against UV light exposure during band excision
Gel Alignment Templates, for rapid, precise positioning of gels and blots on sample trays
Standard 302nm UV Lamps, pkg of 6, replacement UV lamps
Mitsubishi Thermal Printer Paper, pkg of 4, printer paper rolls for use with Mitsubishi thermal printerXcitaBlue Viewing Goggles, pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays

12005137
12003026
12003027
12003028
1708377
12003914
12003915
1708097
17075811708185

In the U.S., call toll free at 1-800-424-6723

Imaging Instruments, Analysis, and Software | 285

Imaging Systems
www.bio-rad.com/imaging

See Also
Protein gel stains:page 221.
Nucleic acid gel stain:
page 264.
Gel analysis software:
page 289.
ReadyAgarose precast
gel system: page 260.
Western blotting:page 232.

ChemiDoc XRS+ System
The ChemiDoc XRS+ system is based on CCD highresolution, high-sensitivity detection technology andprovides application flexibility. Key benefits include:
■

■

■

■

 ccurate, protocol-driven gel and blot imaging
A
and analysis
 utomated quantitative analysis of protein and DNA
A
samples in seconds
 ide range of applications with special accessories
W
to preserve sample integrity for downstream research
while ensuring user safety
Publication-quality results

ChemiDoc XRS+ System
The ChemiDoc XRS+ system offers sensitive
chemiluminescence detection in addition to gel and blot
documentation of fluorescent and colorimetric samples.
The system includes a sensitive 16-bit CCD camera thatis cooled for detection of faint samples. The XRS+ system
is compatible with Stain-Free technology, which offers
checkpoints in western blot experiments and a convenient
total protein loading control for data normalization.
For More Information
Web: bio-rad.com/imagingsystems
Request or download bulletin: 5837

Ordering Information
Catalog #Description

1708265

 hemiDoc XRS+ System with Image Lab Software, compatible with PC or Mac, includes
C
darkroom hood, UV transilluminator, epi-white illumination standard filter, camera, power supply,
cables, Image Lab software

Accessories
1708256
1708299
1708289
17080811708097
170758117081821708008
1703759
1703760
1708184
1708026
1708279
1708185

Price, U.S. $InquireChemiDoc XRS+ IQ/OQ, for use with Image Lab software

1,760.00

Image Lab Upgrade for ChemiDoc XRS+ System, upgrade package for ChemiDoc XRS+ System
White Light Conversion Screen, for use with non–touch screen ChemiDoc MP, ChemiDoc XRS+,and Gel Doc XR+ systems
Filter, standard emission, 62 mm
Standard 302 nm UV Lamps, 6 replacement bulbs
Mitsubishi Thermal Printer Paper, 4 rolls, for use with Mitsubishi thermal printerXcitaBlue Conversion Screen Kit, includes viewing goggles and standard detection filter
Fluorescence Reference Plate
Bio-Rad Fluorescent Ruler
Gel Cutter Ruler
Gel Alignment Templates, pkg of 3, templates for aligning gels and blots, for use with ChemiDoc XRS+and Gel Doc XR+ systems
Focus Calibration Target
UV Safety Shield for ChemiDoc XRS+, pkg of 1, to protect users from UV light exposure during band excision
XcitaBlue Viewing Goggles, pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays

905.00555.00286 | Imaging Instruments, Analysis, and Software

400.00335.00155.001,705.00
120.00
21.00
21.00
48.00
86.00

In the U.S., call toll free at 1-800-424-6723

Imaging Systems
bio-rad.com/imaging

See Also

GelDoc Go Imaging System

Stain-Free gels(mini, midi formats):
pages 196, 203.

The GelDoc Go imaging system is a small, easy-to-use
gel documentation system. Built with a large UVtransilluminator, the GelDoc Go system offers the flexibility
to image and excise bands from many different sizes
and types of gels including traditional nucleic acid dyes
(ethidium bromide) or SYBR® Safe dyes.

Protein Electrophoresis:
page 180
Nucleic Acid
Electrophoresis:
page 254

In just three clicks via Image Lab Touch software, you cansimplify your gel documentation process but still remain incontrol of you research. Enjoy quick onboard software anda free, robust analysis package. Benefits include:
■

■

■

■

■

■

■

Video
Introducing the
GelDoc Go

Simplicity — use it right out of the box; no training or
installation required mall footprint, large imaging area — do everythingS
you need with minimal bench space

bio-rad.com/
GDGOintro

Flexibility — image gels and colorimetric blots ofvarying sizes and stains
Band excision — cut gels with a UV safety shield or use
a blue tray to preserve DNA samples
Stain-Free technology — convert a 2 hr Coomassie
staining protocol into a 1 min visualization step; nostaining or destaining required

For More Information
Web: bio-rad.com/geldocgo
Download bulletin: 7284

Easy user interface — Image Lab Touch software
enables simple and fast acquisition, user accounts, data
export options, and more
 icense-free analysis software — Image Lab softwareLis a robust, well established analysis package available
without a license for PC and Mac. Security Edition (CFR)
is available as an upgrade (see pg 289)

Ordering Information
Catalog #Description

12009077
12012147

GelDoc Go Imaging System, small, easy-to-use gel documentation system, built to accommodate
large gels and band excision
IQ/OQ Kit, installation and operational qualification kit, includes protocols and imaging target

12012189
12012165
12012160
12012164
12012190
17081851708377
1703760

UV Sample Tray, primary tray for nucleic acid gels, Stain-Free gels and colorimetric blots
White Sample Tray, for white light applications
Blue Sample Tray, an alternative to UV illumination to preserve DNA samples (SYBR applications)UV Shield, to protect against UV light exposure during band excision
GelDoc Go Gel Alignment Template Kit
XcitaBlue Viewing Goggles, pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays
Holder for Sample Trays and UV Shield, pkg of 1, holds 3 sample trays and 1 UV shield
Gel Cutter Ruler, pkg of 1, accessory for use with gel excision

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Inquire1,760.00
905.00555.00400.00335.00

Imaging Instruments, Analysis, and Software | 287

Imaging Systems
bio-rad.com/imaging

See Also
Mini-format verticalelectrophoresis:
page 192.
Midi-format verticalelectrophoresis:
page 201.
Protein standards:
page 187.
QC colloidal
Coomassie stain:
page 221.

GS-900 Calibrated Densitometer
The GS-900 calibrated densitometer delivers superior
accuracy, sensitivity, and data reproducibility. To ensure
the accuracy of each scan, the GS-900 calibrated
densitometer contains an internal optical density tablet,which is scanned and used for calibration before each run.
Features include:■

Image Lab software:
page 289.

■

■

■

■

■

■

■

 ransmissive and reflective imaging using red, green,Tand blue CCD technology to optimally scan andquantitate colorimetric blots and gels treated with avariety of stains
 ccurate quantitation of samples over a large dynamicArange (up to 3.4 OD) ensuring detection of both highly
abundant and dilute proteins canning of larger gels for enhanced separation ofS
proteins on oversized 29 x 33 cm imaging areaIQ/OQ kit available for validation of the calibration
functions using a NIST-traceable external target toconfirm the accuracy of the internal target, guaranteeing
accurate and reproducible results
 igh resolution and analysis of closest bands on a gelH
due to 16-bit precision and 36.3 µm resolution
 ealed imaging area to accommodate wet samples ofSvariable thickness
 urity analysis and lane background tools forP
manufacturing QC
 .S. FDA 21 CFR Part 11 regulation complianceUsoftware available

For More Information
Web: bio-rad.com/gs900
Download bulletin: 6385

Ordering Information
Catalog #Description

1707989

GS-900 Calibrated Densitometer, PC-compatible calibrated densitometer, cables, Image Lab software

Inquire1707991

 S-900 Calibrated Densitometry System, PC-compatible calibrated densitometer, cables,
G
Image Lab software, Biologics Analysis Workflow Starter Kit (Criterion cell, Criterion TGX precast gels,
Precision Plus Protein standards, QC colloidal Coomassie stain, buffers)
GS-900 Regulatory Tools Package, includes GS-900 IQ/OQ Kit and Image Lab Security Edition 21 CFRPart 11 module, 1 license
GS-900 IQ/OQ Kit, set of protocols for installation qualification/operational qualification and NIST-traceable
external step tablet for the GS-900 calibrated densitometer

8,400.00

1707993
1707994288 | Imaging Instruments, Analysis, and Software

Price, U.S. $5,140.00

In the U.S., call toll free at 1-800-424-6723

Blot and Gel Image Analysis Software
bio-rad.com/imagelabsoftware

Blot and Gel Image Analysis Software

See Also
Imaging systems:
page 284.
Bio-Plex Manager
software:page 296.

Image Lab Software
Image Lab is a family of software packages that provides
simple application-driven image acquisition, streamlined
imaging analysis, and features to support better traceability
and data security (U.S. FDA 21 CFR Part 11 compliance).

GS-900 densitometer, and legacy systems. In addition
to easy acquisition it provides algorithm-driven analysis
of gel and blot data using signals. Image Lab desktop is
compatible with PC and Mac operating systems.

Image Lab Touch is the onboard software package forimage acquisition on the ChemiDoc MP, ChemiDoc, and
GelDoc Go imaging systems.

Image Lab software, Security Edition has the samefeatures as Image Lab desktop but also provides
traceability and data integrity in line with U.S. FDA 21 CFRPart 11 regulations. An additional fee is required for the
Security Edition.

Image Lab desktop is the well-established and free Bio-Rad
imaging software package with automated image
acquisition by application for the ChemiDoc XRS+,For More Information
Web: bio-rad.com/imagelabsoftware

Videos
Product
Spotlight

bio-rad.com/
ILtools

Product
Tutorial

bio-rad.com/
ILnormalization

Product
Tutorial

bio-rad.com/
ILdensitometric

Chemiluminescence and Stain-Free blot analysis on Image Lab
desktop software.

Multichannel image acquisition with Image Lab Touch software.
Image Lab Software Selection Guide
Image Acquisition

Image Analysis

FDA 21 CFR Part 11
Compliance

Image Lab Touch

Image Lab Desktop

Image Lab Desktop

Image Lab Security
EditionChemiDoc MP

✓

—

✓

✓

ChemiDoc

✓

—

✓

✓

ChemiDoc XRS+

—

✓

✓

✓

GelDoc Go

✓

—

✓

✓

GS-900—✓✓✓

SystemOrdering Information
Catalog #Description

12012162
12012161
12012150

Image Lab Software, Security Edition for 21 CFR Part 11 compliance, 1 license
Image Lab Software, Security Edition for 21 CFR Part 11 compliance, 5 licenses
Image Lab Software, Security Edition for 21 CFR Part 11 compliance, 10 licenses

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $3,705.00
InquireInquireImaging Instruments, Analysis, and Software | 289

Imaging Instruments, Analysis, and Software
Subject Index

Subject Index
ChemiDoc MP and ChemiDoc Imaging Systems, 285
ChemiDoc XRS+ System, 286
GelDoc Go Imaging System, 287
GS-900 Calibrated Densitometer, 288
Image Lab Software, 289

290 | Imaging Instruments, Analysis, and Software

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System
Bio-Plex Multiplex System
Instruments, Software, and Tools

291
292

Bio-Plex Assays
Bio-Plex Pro Human Inflammation Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays
Bio-Plex Pro Cell Signaling Assays
Bio-Plex Pro RBM Apoptosis Assays
Bio-Plex Pro RBM Kidney Toxicity Assays
Bio-Plex Pro Diabetes Assays
Bio-Plex Pro Human Isotyping Assays
Bio-Plex Pro Mouse Chemokine Panel
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
Bio-Plex Pro Human SARS-CoV-2 Serology Assays
Bio-Plex Pro Human Apolipoprotein Assay Panel
Bio-Plex COOH Beads and Related Reagents

299
299
300
307
310
311
312
314
315
316
317
318
319

Bio-Plex Multiplex System
Instruments, Software, and Tools

bio-rad.com/bio-plex

Bio-Plex Multiplex System
Videos
Product
Demonstration

The Bio-Plex multiplex platform is built on the most flexible technology available. Bio-Rad offers instruments to satisfy avariety of research needs and budgets for multiplex applications. These readers include the versatile Bio-Plex 200 systemand the high-throughput capacity Bio-Plex 3D suspension array system.
Learn More about the Technology
Web: bio-rad.com/tech/bio-plex

bio-rad.com/
bio-plextechnology
overview

Customer
Testimonial

Instruments, Software, and Tools
Bio-Plex System Selection Guide
Bio-Plex 200

Bio-Plex 3D

bio-rad.com/
bio-plexitaly
testimonial

Customer
Testimonial

Features

Versatile — accommodates
multiple users
Flexible — can read both magnetic
and nonmagnetic assays

bio-rad.com/
bio-plexkorea
testimonial

One acquisition and analysis
software package

Meets high-throughput needs
Optimal for labs with fewer
budget restrictions
Offers robotics interfacing

User-friendly data management
Read time (1 x 96-well plate)~45 min~20 minPlate compatibilities

96-well

96- and 384-wellNumber of measurable analytes/well

100

500

Assay compatibility

All magnetic and nonmagnetic(polystyrene) bead-based assays

Most magnetic and all nonmagnetic(polystyrene) bead-based assays

Acquisition software

Bio-Plex Manager

xPONENT

Analysis software

Bio-Plex Manager and
Bio-Plex Data Pro

Bio-Plex Manager and
Bio-Plex Data Pro

On-site training

Included

Included

Footprint (W x D x H)

16.9 x 20.1 x 9.1" (43 x 51 x 23 cm)

23 x 25.7 x 18" (58.4 x 65.3 x 45.7 cm)

292 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System
Instruments, Software, and Tools

bio-rad.com/bio-plex

Bio-Plex 200 System
The Bio-Plex 200 system integrates xMAP suspension
array technology with a reliable instrument, dedicatedsoftware, and validation tools. The system includes:
■

■

■

■

 rray reader — distinguish up to 100 different color-codedAbead sets, each representing a different assay. Results in up
to 9,600 data points in 45 min from a familiar 96-well format
 ptional high-throughput fluidics (HTF) — delivers up
O
to 20 L of sheath fluid (40 plates) without user intervention
 io-Plex MCV plate IV — customized for hands-freeBstartup, shutdown, and performance testingBio-Plex Manager software — controls the instrument,
data acquisition, and analysis (Standard andSecurity Editions)Time-saving features include:■ implified startup, shutdown, control, and pre- andSpost-run maintenance routines■

Sophisticated analysis and instrument control software■

Automated IQ/OQ

■

■

■

Integrated system validation and calibration logs for arecord of system performance uperior curve fitting, statistics reporting, and chartingS
features alibration capabilities for both broad and low rangeCstandard dilutions

For More Information
Web: bio-rad.com/bio-plex200
Download bulletin: 6006

Ordering Information
Catalog #Description

171000201

 io-Plex 200 System, 100–240 V, includes array reader, microplate platform, Bio-Plex Manager softwareBwith workstation license, PC, monitor, calibration kit, validation kit 4.0, MCV plate IV, Bio-Plex reservoir,
20 L sheath fluid, spare sample needle
Bio-Plex 200 System with HTF, same as #171000201 with high-throughput fluidics (HTF)

171000205

Price, U.S. $InquireInquireBio-Plex 200 Accessories and Replacement Parts
171203050
Bio-Plex Reservoir
171000055
Sheath Fluid, 1x, 20 L
171002002
Communication Cable, 3' CAN BUS cable, connects the array reader with the HTF system
171002003
Communication Cable, 5' USB cable, connects the array reader with a PC USB port
171002010
Sheath Fluid Bottle, 1 L
171002012
Sheath Waste Bottle, 1 L
171002020
Sample Needle, 4.6" (11.6 cm)
171002024
Alignment Guide, aligns the array reader on the microplate platform
171002026
Needle Adjustment Wrench
171002030
Protective Shield for Sample Needle
171002032
Air Intake Filter
171002033
Syringe Seal
171002034
Syringe Seal with Cylinder
171002038
Sheath Fluid Filter with Quick Connect Tubing, pkg of 1, replacement maintenance part for internal fluidics
system on the Bio-Plex 200 system; removes >5 μm diameter particles
171002040
Sheath Cube Filter, pkg of 1, replacement maintenance part for use with high-throughput fluidics (HTF)
system and Bio-Plex 200 system; removes >10 µm diameter particles
171002056
HTF Tubing, pkg of 1, air tubing HTF to reader, sheath tubing HTF to reader, tubing sheath container to HTF(including 10 µm filter), waste tubing (reader to waste container)
171002023
Needle Guide, allows sample needle access to microplates

82.00
69.00
305.00320.00420.00420.00310.00250.0098.00
135.00235.0092.00
435.00275.00230.00490.00185.00

Bio-Plex 200 System Performance Validation and Calibration Tools
171203001
Bio-Plex Validation Kit 4.0, includes optics validation, reporter validation, classify validation, andfluidics validation bead sets for approximately 50 validation routines using Bio-Plex Manager software,
current version (Standard or Security Edition), and MCV plate IV
171203033
Bio-Plex MCV Plate IV, for use with Bio-Plex Manager software, current version(Standard or Security Edition), and validation kit 4.0
171203060
Bio-Plex Calibration Kit, includes Cal1 and Cal2 calibration beads for approximately 50 daily calibration routines

In the U.S., call toll free at 1-800-424-6723

1,765.00

180.00580.00

Bio-Plex Multiplex System | 293

Bio-Plex Multiplex System
Instruments, Software, and Tools

bio-rad.com/bio-plex

Bio-Plex 3D Multiplex System
The Bio-Plex 3D multiplex system is the next-generation
multiplexing platform based on xMAP technology. Expanded
multiplexing capability, faster time to results, and automation
capability make it the platform of choice for high-throughput
testing for nucleic acid and protein applications.
Features include:■ apid read times — twice as fast as with theR
Bio-Plex 200 system■

Measurement of up to 500 unique analytes in a single sample■

96- and 384-well plate capability■

 aboratory information system (LIMS/LIS)–compatibleLsoftware■Automation-compatible tray design and software■

Robotics interfacing capabilities■

Compatibility with magnetic or nonmagnetic assays■

Bio-Plex Manager software for data analysis■

On-site training

For More Information
Web: bio-rad.com/bio-plex3D
Download bulletins: 5967 and 5980

Bio-Plex 3D Suspension Array System with Bio-Plex Manager Software

System Throughput Capabilities
Test (50 μl well volume,2,500 beads/well)

Read Time*

Tests/Hr

96-well (100-plex)
384-well (100-plex)
96-well (500-plex)
384-well (500-plex)

18 min1 hr 15 min
45 min2 hr 15 min

32,00030,70064,00085,000* Read times measured across four instruments. Actual results may vary.

Ordering Information
Catalog #Description

BioPlex3D

 io-Plex 3D Multiplex System, includes Bio-Plex 3D multiplex system, xPONENT acquisition software, PC,Bcalibration and verification reagents, Bio-Plex Manager desktop licensexPONENT 21 CFR Part 11 Software Module, enables U.S. FDA 21 CFR Part 11 compliancexPONENT Automation Module, enables interfacing with robotics workstations
Swivel Base, allows rotation of the instrument away from the robotics workstation for maintenance andsample loadingxPONENT LIS Software Module, enables interfacing with LIMS/LIS databases
xPONENT Extra Seat Licenses, 3 additional seats of xPONENT software
Bio-Plex 3D Calibration Kit, good for 25 uses; includes reagents, CD, 25 stripwells
B io-Plex 3D Performance Verification Kit, good for 25 uses; kit includes reagents, fluidics, CD, 25 stripwells

892018500001*
892018700001*
171022001*
892018600001*
892018200001*
FM3DCALK25
FM3DPVERK25

Price, U.S. $InquireInquire2,745.00
Inquire1,110.00
1,110.00
900.001,040.00* All additional accessories may be purchased through Bio-Rad Laboratories, Inc. at the time of system purchase.
Post–system purchase accessories must be purchased directly from Luminex Corporation.

294 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System
bio-rad.com/bio-plex

Instruments, Software, and Tools

Bio-Plex Pro Washing Accessories
Automated Bio-Plex Pro Wash Station
The Bio-Plex Pro wash station streamlines multiplex assays
by eliminating manual wash steps. Bio-Plex Pro wash
stations help decrease variability and increase consistency inresults between experiments and are specifically designed toperform Bio-Plex assay wash steps. Benefits include:■

Reliable and reproducible results■

Optimized onboard protocols■

Simple ELISA-like workflow

The Bio-Plex Pro wash station incorporates a magnetic
plate carrier for simple and reliable hands-free washing of
96-well plates.
Wash stations include preset wash programs that havebeen optimized for Bio-Plex assays.
Bio-Plex Handheld Magnetic Washer
The Bio-Plex handheld magnetic washer is used in manual
wash steps for all Bio-Plex magnetic assays.

Ordering Information
Catalog #Description

30034376

 io-Plex Pro Wash Station, includes magnetic plate carrier, waste bottle, 2 buffer bottles for use withB
magnetic beads
Bio-Plex Handheld Magnetic Washer, includes magnetic washer and adjustment hex tools for washing all
Bio-Plex magnetic assays

171020100
Accessories
171025001
171304500
171304502

 io-Plex Pro Flat Bottom Plates, 40 x 96-well plates, for washing magnetic beads using the Bio-Plex Pro
B
or Bio-Plex Pro II wash stations
Bio-Plex Wash Buffer, 1.5 L
Filter Plate, 1 x 96-well filter plate with clear plastic lid, plate holder tray, for washing nonmagnetic beads
using the Bio-Plex Pro II wash station, optional vacuum manifold; sealing tape not included

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Inquire760.00240.0096.00
27.00

Bio-Plex Multiplex System | 295

Bio-Plex Multiplex System
Instruments, Software, and Tools

bio-rad.com/bio-plex

Bio-Plex Manager Software, Standard Edition
Bio-Plex Manager software is a comprehensive, all-in-one
software package that provides advanced data analysis for
multiplex assays.
Simple Operation
Ability to self train — just follow the steps outlined on
the screen

Data Export, Cross-Platform Capabilities, andSystem Compatibility
Import and analyze data from xPONENT and otherthird-party software
■

■

■

■

 amiliar Windows interface — no new terms or menusF
to learn

Save TimeSimplified input — values for standards are easy to enter;automatic addition of new lots reduces setup time

■

■

■

■

■

■

 utomated data processing — proprietary algorithmAautomatically sets the appropriate dynamic range andidentifies data points outside this range
Immediate recalculation of results efficiently updates datawhen settings are changed

Use Tools That Meet Your Unique Needs
Superior sensitivity and dynamic range with onboard
Brendan Scientific StatLIA 4PL and 5PL weighted
curve-fitting models■■■

Data normalization using multiple internal controls(housekeeping genes) for gene expression applications
 ene Manager analysis module for presence and absenceG
analysis, pathogen detection, and genotyping applications

Analyze Data with Easy-to-Use Reports
Automated export of standard curve graphs■

 se any commercial or custom xMAP assay built on
U
any Luminex microsphere opy and paste data into popular word processingCand presentation software applications
Export to text, XML, and Excel in column or 96-well format
 nalyze data in LIMS and other third-party software byAcustomizing export options
 equest Bio-Plex Manager 4.1.1 software forRinstallation on Luminex systems with IS 2.3 software

Bio-Plex Manager Software Licenses
LicenseCapabilities and Uses

Instrument control	Full-system, data acquisition and analysis; included
with all Bio-Plex 200 systems
Desktop	Single-user license for protocol setup and data
analysis; does not control the array reader
Network	Multiuser (5, 10, or 25) licenses for data accessfrom a server; does not control the array reader
Security Edition

Enables U.S. FDA 21 CFR Part 11 compliance

System Requirements
CPU
Pentium 4 or higher, PC only
Operating system Windows XP Professional, Windows 7, Windows 10■■

View of all wells or summary (average) of replicate samples■

Data organized by sample type, group, or well■One-click navigation between analytes■

Flexible data presentation

For More Information
Web: bio-rad.com/bio-plexmanager
Download bulletin: 5613

Ordering Information
Catalog #Description

171STND01

 io-Plex Manager Software, 1-user desktop license to analyze Bio-Plex data and generate protocols,Bdoes not operate the instrument
Bio-Plex Manager Software, 5-user desktop license to analyze Bio-Plex data and generate protocols,
does not operate the instrument
Bio-Plex Manager Software, 10-user desktop license to analyze Bio-Plex data and generate protocols,
does not operate the instrument
Bio-Plex Manager Software, 25-user desktop license to analyze Bio-Plex data and generate protocols,
does not operate the instrument

171STND05
171STND10171STND25

Price, U.S. $InquireInquireInquireInquireContact Bio-Rad Technical Support to verify whether your current system is compatible with these software products.

296 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System
Instruments, Software, and Tools

bio-rad.com/bio-plex

Bio-Plex Manager Software Conversions and Upgrades
Bio-Plex Manager software conversions and upgrades
allow you to upgrade previous versions of Bio-Plex
Manager software or convert non Bio-Plex systems to
run like a Bio-Plex 200 system.

■■ reprogrammed routine processes — automatePstartup, calibration, validation, run, and shutdown nattended routine operations — program preU
and post-run maintenance operations to save time

Ordering Information
Catalog #Description

171STND23

 io-Plex Manager Software for IS 2.3 System, system license most current version, for converting Luminex ISB
and xPONENT systems
B io-Plex Manager Software for 3.0 Instrument Control Software, system license for upgrading standard or
security software version 3.0 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
Bio-Plex Manager Software for 4.0 Instrument Control Software, system license for upgrading the standard or
security software version 4.0 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
Bio-Plex Manager Software for 4.1 Instrument Control Software, system license for upgrading the standard or
security software version 4.1 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
Bio-Plex Manager Software for 5.0 Instrument Control Software, system license for upgrading the standard or
security software version 5.0 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)

171SUPG30
171SUPG40
171SUPG41
171SUPG50

Price, U.S. $Inquire4,875.00
1,305.00
870.00870.00

Bio-Plex Manager Software, Security Edition
Includes all Standard Edition software features and has builtin security features for U.S. FDA 21 CFR Part 11 compliance:■

 ontrol user log-in with different user levels for accessC
to different functions■

Audit trails■

Secure protocol and data files■

 ecure databases for reporting of calibration, validation,Sand instrument operations

For More Information
Web: bio-rad.com/bio-plexmanager
Download bulletin: 5613

Electronic signature window allows you to bring documents into
a secure environment.

Ordering Information
Catalog #Description

171SCRT00

 io-Plex Manager Instrument Control License, Security Edition, upgrades workstation withB
Bio-Plex Manager software, allows compliance with U.S. FDA 21 CFR Part 11 regulations
Bio-Plex Manager Software, Security Edition desktop, includes 1-user desktop license,
allows compliance with U.S. FDA 21 CFR Part 11 regulations. Does not operate the instrument.
Bio-Plex Manager Software, Security Edition desktop, includes 5-user network license,
allows compliance with U.S. FDA 21 CFR Part 11 regulations. Does not operate the instrument.

171SCRT01
171SCRT05

Price, U.S. $InquireInquireInquireContact Bio-Rad Technical Support to verify whether your current system is compatible with these software products.

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 297

Bio-Plex Multiplex System
Instruments, Software, and Tools

Bio-Plex Data Pro SoftwareVideo
Product
Demonstration

bio-rad.com/
bio-plexdatapro
overview

bio-rad.com/bio-plex

Bio-Plex Data Pro software simplifies the management andanalysis of large datasets generated from multiplex assays.
The software allows up to ten data file imports per project;optional Bio-Plex Data Pro Plus software is available forprojects with more than ten data file imports.
Organize Data
Merge, import, sort, and filter data from multiple datasets■

Automate Calculations
Perform common calculations and statistical tests
quickly and confidently■

Visualize Results
Present data clearly with customized graphs and tables■

For More Information
Web: bio-rad.com/datapro

Ordering Information
Catalog #Description

12015422

Bio-Plex Data Pro Software, includes 5 Bio-Plex Data Pro software licenses

298 | Bio-Plex Multiplex System

Price, U.S. $6,170.00

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro Human Inflammation Assays

bio-rad.com/bio-plex

Bio-Plex Assays
Bio-Plex Pro Human Inflammation Assays
Human Inflammation Assays
The Bio-Plex Pro human inflammation assays consist of
37 assays for biomarkers associated with inflammation
pathways and consist of a 37-plex and Treg cytokine
12-plex assay and 37 singleplex sets that can be combined
for a custom multiplex kit.
The assays are built on magnetic beads to enable robust
quantification of multiple proteins in human serum, plasma,and cell culture media samples. These assays are perfectfor broad research applications, including academic andpreclinical studies in:■■■

Key features include:■

Reproducibility — validated to meet analytical standards■

Speed to results — Up to 2,886 data points in 4 hours■

 lexible ordering options — order either premixedF
all-in-one kits or singleplex sets, or customize
your assay with the Bio-Plex assay builder(bio-rad.com/assaybuilder)

Inflammation
Autoimmune diseases
Cancer research

The Bio-Plex Pro human inflammation panel includes the following targets:
APRIL / TNFSF13
BAFF / TNFSF13B
sCD30 / TNFRSF8
sCD163 Set
Chitinase 3-like 1
gp130 / sIL-6Rb
IFN-a2
IFN-b
IFN-g
IL-2

sIL-6Ra
IL-8
IL-10
IL-11
IL-12 (p40)IL-12 (p70)
IL-19
IL-20
IL-22
IL-26

IL-27 (p28)IL-28A / IFN-l2
IL-29 / IFN-l1
IL-32
IL-34
IL-35
LIGHT / TNFSF14
MMP-1
MMP-2
MMP-3

Osteocalcin
Osteopontin
Pentraxin-3
sTNFR-1
sTNFR-2
TSLP
TWEAK / TNFSF12

For More Information
Web: bio-rad.com/bio-plexhumaninflammation
Request or download bulletin: 6625

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro Human Inflammation Premixed All-in-One Kits
171AL001M
Bio-Plex Pro Human Inflammation Panel 1, 37-Plex, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, control, buffers, diluents, streptavidin-PE, 96-well plate, and sealing tape, for the detectionof 37 human inflammation biomarkers
171AL003M
Bio-Plex Pro Human Inflammation Treg Cytokine Panel, 1 x 96-well, includes coupled magnetic beads,
detection antibodies, standards, control, buffers, diluents, streptavidin-PE, 96-well plate, and sealing tape,for the detection of 12 human Treg biomarkers

5,185.00

2,225.00

continues

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 299

Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

bio-rad.com/bio-plex

Ordering Information
Catalog #Description

Bio-Plex Pro Human Inflammation Singleplex Sets
171BL001MAPRIL / TNFSF13 Set
171BL002M
BAFF / TNFSF13B Set
171BL003M
sCD30 / TNFRSF8 Set
171BL004M
sCD163 Set
171BL005M
Chitinase 3-Like 1 Set
171BL006M
gp130 / sIL-6Rb Set
171BL007M
IFN-a2 Set
171BL008M
IFN-b Set
171BL009M
IFN-g Set
171BL010M
IL-2 Set
171BL011M
sIL-6Ra Set
171BL012M
IL-8 Set
171BL013M
IL-10 Set
171BL014M
IL-11 Set
171BL015M
IL-12 (p40) Set
171BL016M
IL-12 (p70) Set
171BL017M
IL-19 Set
171BL018M
IL-20 Set
171BL019M
IL-22 Set
171BL020M
IL-26 Set
Standards
171DL0001
171DL0050

Price, U.S. $215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00171BL021M
171BL022M
171BL023M
171BL024M
171BL025M
171BL026M
171BL027M
171BL028M
171BL029M
171BL030M
171BL031M
171BL032M
171BL033M
171BL034M
171BL035M
171BL036M
171BL037M
171DL0001
171DL0050

IL-27 (p28) Set
IL-28A / IFN-l2 Set
IL-29 / IFN-l1 Set
IL-32 Set
IL-34 Set
IL-35 Set
LIGHT / TNFSF14 Set
MMP-1 Set
MMP-2 Set
MMP-3 Set
Osteocalcin Set
Osteopontin Set
Pentraxin-3 Set
sTNFR-1 Set
sTNFR-2 Set
TSLP Set
TWEAK / TNFSF12 Set
Std, 1PK
Std, 50PK

Bio-Plex Pro Human Inflammation Panel 1, 37-Plex Standard, pkg of 1 vial, lyophilized
mixture of 37 standard analytes
Bio-Plex Pro Human Inflammation Panel 1, 37-Plex Standard, pkg of 50 lot-matched vials,
lyophilized mixture of 37 standard analytes

215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00310.00InquireInquireReagent Kit — for use with human inflammation singleplex sets
171304090M
Bio-Plex Pro Reagent Kit III, 1 x 96-well, includes detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealingtape, for magnetic separation methods

Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays
Bio-Plex Pro cytokine, chemokine, and growth factor assays are magnetic bead–based multiplex immunoassays offering
accurate and reproducible measurements of multiple analytes simultaneously. These assays have been developed to provide
reliable performance with the flexibility required to meet all of your research needs. Assays are available for human, mouse,or rat studies.
Key features includes:
■

■

■

■

Magnetic beads — automated plate washing andimproved reproducibility across experiments
Increased productivity — measure multiple analytes
simultaneously, get results in about 3 hours
 lexible ordering options — order either premixedF
all-in-one kits or singleplex sets, or customize
your assay with the Bio-Plex assay builder(bio-rad.com/assaybuilder)

300 | Bio-Plex Multiplex System■

Cross-platform compatibility — can be used withall xMAP life science instruments supplied by any
Luminex partner
 onvenient pathway panels — Th17, Th1/Th2,
C
and TGF-b available

For More Information
Web: bio-rad.com/CCGFassays
Download bulletins: 5828, 6100, and 6110
Request or download bulletin: 6054

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

bio-rad.com/bio-plex

Human Assays
Human Assays — Available Analytes
6Ckine / CCL21
Basic FGF
BCA-1 / CXCL13
CTACK / CCL27*
ENA-78 / CXCL5
Eotaxin / CCL11*
Eotaxin-2 / CCL24
Eotaxin-3 / CCL26
Fractalkine / CX3CL1
GCP-2 / CXCL6
G-CSF
GM-CSF*
Gro-a / CXCL1*
Gro-b / CXCL2
HGF
I-309 / CCL1
ICAM-1
IFN-a2
IFN-g*

IL-1a
IL-1b*
IL-1raIL-2*
IL-2Ra
IL-3
IL-4*
IL-5
IL-6*
IL-7
IL-8 / CXCL8*
IL-9
IL-10*
IL-12 (p40)IL-12 (p70)
IL-13
IL-15
IL-16*
IL-17A

IL-18
IP-10 / CXCL10*
I-TAC / CXCL11
LIF
MCP-1 / CCL2*
MCP-2 / CCL8
MCP-3 / CCL7*
MCP-4 / CCL13
M-CSF
MDC / CCL22
MIF*
MIG / CXCL9*
MIP-1a / CCL3*
MIP-1b
MIP-1d / CCL15
MIP-3a / CCL20
MIP-3b / CCL19
MPIF-1 / CCL23
b-NGF

PDGF-BBRANTESSCF
SCGF-bSCYB16 / CXCL16
SDF-1aSDF-1a+b / CXCL12TARC / CCL17
TECK / CCL25
TGF-b1
TGF-b2
TGF-b3
TNF-a*TNF-b
TRAIL
VCAM-1VEGF*A nalyte is present in multiple panels. If multiplexing, select analytes from the same panel to avoid cross reactivity.

Ordering Information
Catalog #Description

Price, U.S. $Human Cytokine Premixed All-in-One Kits*
12007283
Bio-Plex Pro Human Cytokine 48-Plex Panel, 1 x 96-well, includes coupled magnetic capture beads, premixed
detection antibodies, standards, quality control, detection antibody diluent HB, standard diluent HB, sample diluent
HB, assay buffer, 10x wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions for thedetection of FGF basic, Eotaxin, G-CSF, GM-CSF, IFN-g, IL-1b, IL-1ra, IL-1a, IL-2Ra, IL-3, IL-12 (p40), IL-16, IL-2, IL-4,
IL-5, IL-6, IL-7, IL-8, IL-9, GRO-a, HGF, IFN-a2, LIF, MCP-3, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1 / CCL2,
MIG, b-NGF, SCF, SCGF-b, SDF-1a, MIP-1a, MIP-1b, PDGF-BB, RANTES, TNF-a, VEGF, CTACK, MIF, TRAIL,
IL-18, M-CSF, TNF-b
M50000007A
Bio-Plex Pro Human Cytokine 8-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection antibodies,
standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate, sealing tape,standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, IFN-g, TNF-a
M5000031YV
Bio-Plex Pro Human Cytokine 17-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10,
IL-12 (p70), IL-13, IL-17, G-CSF, GM-CSF, IFN-g, MCP-1, MIP-1b, TNF-a
M500KCAF0Y
Bio-Plex Pro Human Cytokine 27-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9,
IL-10, IL-12 (p70), IL-13, IL-15, IL-17, basic FGF, eotaxin, G-CSF, GM-CSF, IFN-g, IP-10, MCP-1, MIP-1a, MIP-1b,
PDGF-BB, RANTES, TNF-a, VEGF
M5000005L3
Bio-Plex Pro Human Cytokine Th1/Th2 Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,sealing tape, standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, GM-CSF,
IFN-g, TNF-a
12007975
Bio-Plex Pro Human Immunotherapy 20-Plex Panel, 1 x 96-well, includes coupled magnetic capture beads,
premixed detection antibodies, standards, quality control, detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, 10x wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, andinstructions for the detection of GM-CSF, IFN-g, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, IL-15, IL-17A, IL-18, IP-10,
MCP-1, MIG, MIP-1a, MIP-1b, RANTES, TNF-a

1,605.00

2,710.00

4,085.00

1,820.00

continues

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 301

Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

bio-rad.com/bio-plex

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro Human Cytokine Singleplex Sets, Group I and II, 1 x 96-Well**
171B5001M
IL-1b Set
215.00
171B5026M
171B5002M
IL-1ra Set
215.00
171B5027M
171B5003M
IL-2 Set
215.00
171B6001M
171B5004M
lL-4 Set
215.00
171B6002M
171B5005M
IL-5 Set
215.00
171B6003M
171B5006M
lL-6 Set
215.00
171B6004M
171B5007M
IL-7 Set
215.00
171B6005M
171B5008M
IL-8 Set
215.00
171B6006M
171B5009M
IL-9 Set
215.00
171B6007M
171B5010M
IL-10 Set
215.00
171B6008M
171B5011M
IL-12 (p70) Set
215.00
171B6009M
171B5012M
IL-13 Set
215.00
171B6010M
171B5013M
IL-15 Set
215.00
171B6011M
171B5014M
IL-17A Set
215.00
171B6012M
171B5015M
Eotaxin Set
215.00
171B6013M
171B5016M
Basic FGF Set
215.00
171B6014M
171B5017M
G-CSF Set
215.00
171B6015M
171B5018M
GM-CSF Set
215.00
171B6016M
171B5019M
IFN-g Set
215.00
171B6017M
171B5020M
IP-10 Set
215.00
171B6018M
171B5021M
MCP-1 Set
215.00
171B6019M
171B5022M
MIP-1a Set
215.00
171B6020M
171B5023M
MIP-1b Set
215.00
171B6021M
171B5024M
PDGF-BB Set
215.00
171B6022M
171B5025M
RANTES Set
215.00

TNF-a Set
VEGF Set
IL-1a Set
IL-2Ra Set
IL-3 Set
IL-12 (p40) Set
IL-16 Set
CTACK Set
GRO-a Set
HGF SetICAM-1 Set
IFN-a2 Set
LIF SetMCP-3 Set
M-CSF Set
MIF SetMIG Setb-NGF Set
SCF Set
SCGF-b Set
SDF-1a Set
TNF-b Set
TRAIL Set
VCAM-1 Set

215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00Note: IL-18 is available only in multiplex format.
Standards
12007919
12007920

Bio-Plex Pro Human Cytokine Screening Panel Standards, pkg of 1 vial, cytokine standard for detecting
50 human cytokines
Bio-Plex Pro Human Cytokine Screening Panel Standards, pkg of 50 vials, cytokine standard for detecting
50 human cytokines

Reagent Kit — for use with human cytokine singleplex setsBio-Plex Pro Reagent Kit III, 1 x 96-well, includes detection antibody diluent HB,
171304090M
standard diluent HB, sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE,
flat bottom plate, sealing tape, for magnetic separation methods
Human Th17 Premixed All-in-One Kit
Bio-Plex Pro Human Th17 Cytokine 15-Plex Panel, 1 x 96-well, includes premixed coupled magnetic beads,
171AA001M
detection antibodies, standards, 2-level controls, detection antibody diluent, standard diluent HB, sample diluent
HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for detecting
IL-1b, lL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-g, sCD40L, TNF-a, (IL-17A/F
available as singleplex only)
Bio-Plex Pro Human Th17 Cytokine Singleplex Sets, 1 x 96-Well**
171BA001M
IL-1b
215.00171BA002M
lL-4
215.00171BA003M
lL-6
215.00171BA004M
IL-10
215.00171BA005M
IL-17A
215.00171BA006M
IL-17F215.00171BA007M
IL-21
215.00171BA008M
IL-22
215.00171BA009M
171BA010M
171BA011M
171BA012M
171BA013M
171BA014M
171BA015M
171BA016M

IL-23
IL-25
IL-31
IL-33
IFN-g
sCD40L
TNF-a
IL-17A/F

185.00275.002,720.00

215.00215.00215.00215.00215.00215.00215.00215.00continues

302 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
bio-rad.com/bio-plex

Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

Ordering Information
Catalog #Standards
171DA0001
171DA0501

Description

Price, U.S. $195.007,710.00

Bio-Plex Pro Human Th17 Cytokine Standard, pkg of 1 vial, lyophilized mixture of 16 standard analytes io-Plex Pro Human Th17 Cytokine Standard, pkg of 50 lot-matched vials, lyophilized mixture of 16
B
standard analytes

Reagent Kit III — for use with human Th17 cytokine singleplex sets
171304090M
Bio-Plex Pro Reagent Kit III, 1 x 96-well, includes detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, formagnetic separation methods

385.00

Human Chemokine Premixed All-in-One Kit
171AK99MR2
Bio-Plex Pro Human Chemokine Panel 40-Plex, 1 x 96-well, includes premixed coupled magnetic beads,
detection antibodies, standards, single-level controls, detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, andinstructions, for detecting 40 human chemokines shown below.
Bio-Plex Pro Human Chemokine Singleplex Sets, 1 x 96-Well**
171BK11MR2
6Ckine / CCL21 Set
210.00171BK12MR2
BCA-1 / CXCL13 Set
210.00171BK13MR2
CTACK / CCL27 Set
210.00171BK14MR2
ENA-78 / CXCL5 Set
210.00171BK15MR2
Eotaxin / CCL11 Set
210.00171BK16MR2
Eotaxin-2 / CCL24 Set
210.00171BK17MR2
Eotaxin-3 / CCL26 Set
210.00171BK18MR2
Fractalkine / CX3CL1 Set
210.00171BK19MR2
GCP-2 / CXCL6 Set
210.00171BK21MR2
GM-CSF Set
210.00171BK22MR2
Gro-a / CXCL1 Set
210.00171BK23MR2
Gro-b / CXCL2 Set
210.00171BK24MR2
I-309 / CCL1 Set
210.00171BK25MR2
IFN-g Set
210.00171BK26MR2
IL-1b Set
210.00171BK27MR2
IL-2 Set
210.00171BK28MR2
IL-4 Set
210.00171BK29MR2
IL-6 Set
210.00171BK31MR2
IL-8 / CXCL8 Set
210.00171BK32MR2
IL-10 Set
210.00Standard
171DK0001

171BK33MR2
171BK34MR2
171BK35MR2
171BK36MR2
171BK37MR2
171BK38MR2
171BK39MR2
171BK41MR2
171BK42MR2
171BK43MR2
171BK44MR2
171BK46MR2
171BK47MR2
171BK48MR2
171BK49MR2
171BK51MR2
171BK52MR2
171BK53MR2
171BK54MR2
171BK55MR2

5,520.00

210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00210.00IL-16 Set
IP-10 / CXCL10 Set
I-TAC / CXCL11 Set
MCP-1 / CCL2 Set
MCP-2 / CCL8 Set
MCP-3 / CCL7 Set
MCP-4 / CCL13 Set
MDC / CCL22 Set
MIF SetMIG / CXCL9 Set
MIP-1a / CCL3 Set
MIP-1d / CCL15 Set
MIP-3a / CCL20 Set
MIP-3b / CCL19 Set
MPIF-1 / CCL23 Set
SCYB16 / CXCL16 Set
SDF-1a+b / CXCL12 Set
TARC / CCL17 Set
TECK / CCL25 Set
TNF-a Set

320.00Bio-Plex Pro Human Chemokine Standard, pkg of 1 vial, lyophilized mixture of 40 standard analytes

Reagent Kit III — for use with human chemokine singleplex sets
171304090M
Bio-Plex Pro Reagent Kit III, 1 x 96-well, includes detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, formagnetic separation methods

385.00* Please contact your local Bio-Rad Sales Representative for pricing and availability of 10 x 96-well assay kits.
**	Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 303

Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

bio-rad.com/bio-plex

Mouse Assays
Mouse Assays — Available Analytes
Basic FGF
IFN-g
BCA-1 / CXCL13
IL-1a
CD40L
IL-1b
CTACK / CCL27
IL-2
ENA-78 / CXCL5
IL-3
Eotaxin / CCL11
IL-4
Eotaxin-2 / CCL24
IL-5
Fractalkine / CX3CL1
IL-6
G-CSF
IL-9
GM-CSF
IL-10
Gro / KC
IL-12 (p40)I-309 / CCL1
IL-12 (p70)
ICAM-1
IL-13

IL-15
IL-16
IL-17A
IL-17F
IL-18
IL-21
IL-22
IL-23
IL-25 / IL-17E
IL-27
IL-31
IL-33
IP-10 / CXCL10I-TAC / CXCL11
KC / CXCL1
LIF
M-CSF
MCP-1 / MCAF / CCL2
MCP-2 / CCL8
MCP-3 / CCL7
MCP-5 / CCL12
MDC / CCL22
MIG / CXCL9
MIP-1a / CCL3
MIP-1b / CCL4
MIP-2 / CXCL2

MIP-3a / CCL20
MIP-3b / CCL19
PDGF-BBRANTES / CCL5SCYB16 / CXCL16
SDF-1a / CXCL12TARC / CCL17
TECK / CCL25
TGF-b1
TGF-b2
TGF-b3
TNF-a
VEGF

Ordering Information
Catalog #Description

Price, U.S. $Mouse Cytokine Premixed All-in-One Kits*
M60000007A
Bio-Plex Pro Mouse Cytokine 8-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-2, IL-4, IL-5, IL-10, GM-CSF,
IFN-g, TNF-a
MD000000EL
Bio-Plex Pro Mouse Cytokine 9-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-15, IL-18, basic FGF, LIF, M-CSF,
MIG, MIP-2, PDGF-BB, VEGF
M60009RDPD Bio-Plex Pro Mouse Cytokine 23-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6,
IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17, eotaxin, G-CSF, GM-CSF, IFN-g, KC, MCP-1, MIP-1a, MIP-1b,
RANTES, TNF-a
M6000003J7
Bio-Plex Pro Mouse Cytokine Th1/Th2 Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-5, IL-10, IL-12 (p70),
GM-CSF, IFN-g, TNF-a
Mouse Th17 Premixed All-in-One Kits
M6000007NY
Bio-Plex Pro Mouse Cytokine Th17 6-Plex Group l, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standard, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,sealing tape, standard diluent, sample diluent, and instructions, for the detection of IL-1b, IL-6, IL-10, IL-17A, IFN-g,
TNF-a
12010828
Bio-Plex Pro Mouse Th17 Cytokine 10-Plex, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,sealing tape, standard diluent, sample diluent, for the detection of CD40L, IL-17F, IL-21, IL-22, IL-23, IL-25 / IL17E,
IL-27, IL-31, IL-33, MIP-3a
171GA010M

 io-Plex Pro Mouse Cytokine ICAM-1 Set, 1 x 96-well, includes coupled magnetic beads and detection
B
antibodies for ICAM-1, requires reagent kit V (#12002798) and standards (#171IA0001)

1,625.00

1,895.00

3,490.00

1,635.00

1,310.00

1,865.00

1,865.00
continues

304 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
bio-rad.com/bio-plex

Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro Mouse Cytokine Singleplex Sets, Group I, 1 x 96-Well**
171G5001M
IL-1a Set
215.00171G5002M
IL-1b Set
215.00171G5003M
IL-2 Set
215.00171G5004M
IL-3 Set
215.00171G5005M
IL-4 Set
215.00171G5006M
IL-5 Set
215.00171G5007M
IL-6 Set
215.00171G5008M
IL-9 Set
215.00171G5009M
IL-10 Set
215.00171G5010M
IL-12 (p40) Set
215.00171G5011M
IL-12 (p70) Set
215.00171G5012M
IL-13 Set
215.00Standards
171I50001
171I10501

171G5013M
171G5014M
171G5015M
171G5016M
171G5017M
171G5018M
171G5019M
171G5020M
171G5021M
171G5022M
171G5023M

IL-17A Set
Eotaxin Set
G-CSF Set
GM-CSF Set
IFN-g Set
KC SetMCP-1 Set
MIP-1a Set
MIP-1b Set
RANTES Set
TNF-a Set

171G6005M
171G6006M
171G6007M
171G6008M

MIG SetMIP-2 Set
PDGF-BB Set
VEGF Set

Bio-Plex Pro Mouse Cytokine Standards Group II, pkg of 1 vial, lyophilized mixture of 9 analytes
Bio-Plex Pro Mouse Cytokine Standards Group II, pkg of 50 lot-matched vials, lyophilized mixture of
9 analytes

Bio-Plex Pro Mouse Th17 Cytokine Singleplex Sets, 1 x 96-Well**
171GA001M
CD40L Set
215.00171GA002M
IL-17F Set
215.00171GA003M
IL-21 Set
215.00171GA004M
IL-22 Set
215.00171GA005M
IL-23 Set
215.00171GA006M
IL-25 Set
215.00Standards
171IA0001

Description

Price, U.S. $215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00

Bio-Plex Pro Mouse Cytokine Standards Group I, pkg of 1 vial, lyophilized mixture of 23 analytes
Bio-Plex Pro Mouse Cytokine Standards Group I, pkg of 50 lot-matched vials, lyophilized mixture of 23 analytes

Bio-Plex Pro Mouse Cytokine Singleplex Sets, Group II, 1 x 96-Well**
171G6001M
IL-15 Set
215.00
171G6002M
Basic FGF Set
215.00
171G6003M
LIF Set215.00
171G6004M
M-CSF Set
215.00Standards
171I60001
171I10502

Catalog #171GA007M
171GA008M
171GA009M
171GA010M
171GA011M

IL-27 Set
IL-31 Set
IL-33 Set
ICAM-1 Set
MIP-3a Set

160.007,190.00
215.00215.00215.00215.00115.005,535.00

215.00215.00215.00215.00215.00Bio-Plex Pro Mouse Th17 Cytokine Standards, pkg of 1 vial, lyophilized mixture for detecting 11 analytes

225.00Bio-Plex Pro Reagent Kit V, 1 x 96-well, includes detection antibody diluent HP, standard diluent, sample
diluent, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods
Bio-Plex Pro Reagent Kit V, 10 x 96-well, includes detection antibody diluent HP, standard diluent, sample
diluent, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods
Bio-Plex Pro High Dilution Reagent Kit, 1 x 96-well, includes assay buffer, wash buffer, detection antibody
diluent, streptavidin-PE, flat bottom plate, sealing tape, standard diluent, sample diluent

275.00

Reagent Kits
12002798

12005847

171304080M

2,395.00

275.00

Bio-Plex Pro Mouse Chemokine Panel
12009159
12002247
12002254
12002260
12002798
12002796

Bio-Plex Pro Mouse Chemokine 31-Plex MCP-2 / CCL2 Set, 1 x 96-well
Bio-Plex Pro Mouse Chemokine, MCP-2 / CCL8 Set, 1 x 96-well
Bio-Plex Pro Mouse Chemokine MIP-2 / CXCL2 Set, 1 x 96-well
Bio-Plex Pro Mouse Chemokine TECK / CCL25 Set, 1 x 96-well
Bio-Plex Pro Reagent Kit V, 1 x 96-well
Bio-Plex Pro Mouse Chemokine Standards, 1 pack* Please contact your local Bio-Rad Sales Representative for pricing and availability of 10 x 96-well assay kits.
** Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should not bemixed with others from different panels or groups.

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 305

Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays

bio-rad.com/bio-plex

Rat Assays
Rat Assays — Available Analytes
G-CSF
GM-CSFGro / KC
IFN-g
IL-1a
IL-1b
IL-2
IL-4

IL-5
IL-6
IL-7
IL-10
IL-12 (p40)IL-12 (p70)IL-13
IL-17A

IL-18*
KC
MCP-1
M-CSF
MIP-1aMIP-2
MIP-3aRANTES

TGF-b1
TGF-b2
TGF-b3TNF-a
VEGF* IL-18 is not available as a singleplex.

Ordering Information
Catalog #Description

Price, U.S. $Rat Cytokine Premixed All-in-One Kits*
12005641
Bio-Plex Pro Rat Cytokine 23-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay buffer, wash buffer, detection antibody diluent HP, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of G-CSF, GM-CSF, Gro/KC, IFN-g, IL-1a,
IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, MCP-1, M-CSF, MIP-1a, MIP-3a, RANTES,
TNF-a, VEGF
171K1002M
Bio-Plex Pro Rat Cytokine Th1/Th2 12-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standard, assay buffer, wash buffer, detection antibody diluent HP, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent and instructions, for the detection of IL-a, IL-1b, IL-2, IL-4,
IL-5, IL-6, IL-10, IL-12 (p70), IL-13, GM-CSF, IFN-g, TNF-a
Catalog #Description

Price, U.S. $Bio-Plex Pro Rat Cytokine Singleplex Sets, 1 x 96-Well**
171L1003M
G-CSF Set
215.00171L1004M
GM-CSF Set
215.00171L1005M
Gro / KC Set
215.00171L1006M
IFN-g Set
215.00171L1007M
IL-1a Set
215.00171L1008M
IL-1b Set
215.00171L1009M
IL-2 Set
215.00171L1010M
IL-4 Set
215.00171L1011M
IL-5 Set
215.00171L1012M
IL-6 Set
215.00171L1013M
IL-7 Set
215.00171L1014M
IL-10 Set
215.00Standards
171NZ0001

Catalog #Description

171L1015M
171L1016M
171L1017M
171L1018M
171L1020M
171L1027M
171L1021M
171L1022M
171L1023M
171L1024M
171L1025M
171L1026M

IL-12 (p40) Set
IL-12 (p70) Set
IL-13 Set
IL-17A Set
M-CSF Set
MCP-1 Set
MIP-1a Set
MIP-2 Set
MIP-3a Set
RANTES Set
TNF-a Set
VEGF Set

3,615.00

2,475.00

Price, U.S. $ io-Plex Pro Rat Cytokine Standards, pkg of 1 vial, lyophilized mixture of 31 analytes.
B
Compatible with rat cytokine and rat diabetes assays

215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00215.00225.00

Reagent Kits — for use with rat cytokine singleplex sets
12002798

12005847

 io-Plex Pro Reagent Kit V, 1 x 96-well, includes detection antibody diluent HP, standard diluent, sample
Bdiluent, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods
Bio-Plex Pro Reagent Kit V, 10 x 96-well, includes detection antibody diluent HP, standard diluent, sample
diluent, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods

275.002,395.00* 		Please contact your local Bio-Rad Sales Representative for pricing and availability of 10 x 96-well assay kits.
**	Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.

306 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro Cell Signaling Assays

bio-rad.com/bio-plex

Multi-Species TGF-b Assays

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro TGF-b Premixed All-in-One Kit (compatible with human, mouse, and rat samples)
171W4001M
Bio-Plex Pro TGF-b 3-Plex Assay, 1 x 96-well, includes coupled magnetic beads, detection antibodies,
standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate, sealing tape,standard diluent, sample diluent, for the detection of TGF-b1, TGF-b2, TGF-b3

925.00

Bio-Plex Pro TGF-b Singleplex Sets, 1 x 96-Well*
171V4001M
TGF-b1 Set
171V4002M
TGF-b2 Set
171V4003M
TGF-b3 Set

240.00240.00240.00Standards
171X40001

63.00

171X40501

 io-Plex Pro TGF-b Standard, pkg of 1 vial, lyophilized standard for detecting TGF-b1, TGF-b2, andB
TGF-b3 analytes
Bio-Plex Pro TGF-b Standard, pkg of 50 lot-matched vials, lyophilized standard for detecting TGF-b1,
TGF-b2, and TGF-b3 analytes

4,135.00

Reagent Kits — for use with TGF-b singleplex sets
171304090M
Bio-Plex Pro Reagent Kit III, 1 x 96-well, includes detection antibody diluent HB, standard diluent HB, sample
diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods
12005846
Bio-Plex Pro Reagent Kit III, 10 x 96-well, includes detection antibody diluent HB, standard diluent HB, sample
diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods
Bio-Plex Pro Assay Accessories
171304500
Bio-Plex Wash Buffer, 1.5 L
171025001**
Bio-Plex Pro Flat Bottom Plates, 40 x 96-well plates
171304502
Filter Plate, 1 x 96-well filter plate with clear plastic lid, plate-holder tray, sealing tape not included

275.002,395.00

96.00
240.0027.00*		Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.
** Required for washing Bio-Plex Pro assays or other magnetic bead–based assays using Bio-Plex Pro and Bio-Plex Pro II wash stations.
Note: x-Plex assay panels can be ordered with any combination of available assays. For more information,
go to bio-rad.com/bio-plex/assaybuilder.

Bio-Plex Pro Cell Signaling Assays
Cell Signaling Assays
The Bio-Plex Pro cell signaling assays are magnetic bead–based immunoassays designed to meet the sensitivity needsof the most discerning scientists. The multiplex format enables
robust, reproducible, and simultaneous measurement ofproteins involved in key intracellular signaling pathways.
Choose from an 8-plex Akt panel or a 9-plex MAPK panel.

In the U.S., call toll free at 1-800-424-6723

These assays incorporate several features to enhanceboth quality and ease of use:■■

Assay quick guide to get you started right away
 ssay protocol optimized for exceptional sensitivityAand broad dynamic range

Bio-Plex Multiplex System | 307

Bio-Plex Assays
Bio-Plex Pro Cell Signaling Assays

bio-rad.com/bio-plex

Bio-Plex Pro Cell Signaling Phosphoprotein Assays

Phosphoprotein

Lysate Controls for
Phosphoprotein
AssaysLysate Catalog #Untreated HeLa
Lysate Control
for Total Protein
Assay
Catalog#171YZT002

Bead Region

Compatible Species

Akt Panel
8-plex, catalog#LQ00006JK0K0RR
Akt (Ser473)

EGF-treated HEK-293 171YZ0001*

BAD (Ser136)

PDGF-treated
NIH3T3

GSK-3a/b (Ser21/Ser 9)

EGF-treated HEK-293 171YZ0001*

IRS-1 (Ser636/Ser639)

PDGF-treated
NIH3T3

171YZ0007*

mTOR (Ser2448)

PDGF-treated
NIH3T3

171YZ0007*•

171YZ0007*•

75

H, M, R

26

H, M, R

18

H, M, R

76

H, M, R•

46

H, M, R

p70 S6 kinase (Thr389)

b-NGF-treated PC12

171YZ0006*•

55

H, M, R

PTEN (Ser380)

PDGF-treated
NIH3T3

171YZ0007*•

22

H, M, RS6 ribosomal protein(Ser235/Ser236)

EGF-treated SK-Br3

171YZ0003*

74

H, M, RATF-2 (Thr71)

UV-treated HEK-293

171YZ0009**

Erk1/2 (Thr202/Tyr204,
Thr185/Tyr187)

EGF-treated HEK-293 171YZ0001***

MAPK Panel
9-plex, catalog#LQ00000S6KL81S
20

H, M, R

•

38

H, M

51

H

HSP27 (Ser78)

EGF-treated SK-Br3

171YZ0003**

JNK (Thr183/Tyr185)

UV-treated HEK-293

171YZ0009**

•

34

H

MEK1 (Ser217/Ser221)

EGF-treated HEK-293 171YZ0001***•

27

H, M, R

p38 MAPK (Thr180/Tyr182)

UV-treated HEK-293

171YZ0009**•

36

H, M, R

p53 (Ser15)

UV-treated HEK-293

171YZ0009**

53

H

p90 RSK (Ser380)

EGF-treated SK-Br3

171YZ0003***35

H, M, R

Stat3 (Ser727)

IFN-a-treated Hela

171YZ0004**

52

H, M, R

Bio-Plex Pro Housekeeping ProteinsHousekeeping Protein

Singleplex Cat.

Lysate Control

Human GAPDH

171V60019M

Untreated HeLa

Catalog
171YZT002

b-Actin

171V60020M

Untreated HeLa

171YZT002

Negative control for all
housekeeping protein assays—

Detection antibody diluent—* Included in LQ00006JK0K0RR.
** Included in LQ00000S6KL81S.
*** Included in LQ00000S6KL81S and LQ00006JK0K0RR.
For More Information
Web: bio-rad.com/cellsignalingassays
Download bulletin: 5483
Request or download bulletin: 5405

308 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro Cell Signaling Assays

bio-rad.com/bio-plex

Ordering Information
Catalog #Premixed All-in-One Kits
LQ00006JK0K0RR

LQ00000S6KL81S

Description
Bio-Plex Pro Cell Signaling Akt Panel 8-Plex Assay, 1 x 96-well, includes coupled magnetic beads anddetection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, plate seals,
for the detection of Akt (Ser473), BAD (Ser136), GSK-3a/b (Ser21/Ser9), IRS-1 (Ser636/Ser639), mTOR (Ser2248),
p70 S6 kinase (Thr389), PTEN (Ser380), S6 ribosomal protein (Ser235/Ser236)
Bio-Plex Pro Cell Signaling MAPK Panel 9-Plex Assay, 1 x 96-well, includes coupled magnetic beads anddetection antibodies, buffers, lysis factor QG, diluent, streptavidin-PE, 96-well flat bottom plate, and plate seals
for the detection of ATF-2 (Thr71), ErK1/2 (Thr202/Tyr204, Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185),
MEK1 (Ser217/Ser221), p38 MAPK (Thr180/Tyr182), p53 (Ser15), p90 RSK (Ser380), Stat3 (Ser727)

Housekeeping Protein Singleplex Sets*
171V60019M
Human GAPDH Set

300.00

171V60020M

b-Actin Set

Bio-Plex Pro Cell Signaling Reagents — cell signaling reagent kits are required to run the singleplex sets
171304006M
Bio-Plex Pro Cell Signaling Reagent Kit, 1 x 96-well, includes assay cell lysis buffer, cell lysis factor QG, cell wash
buffer, bead resuspension buffer, detection antibody diluent, wash buffer, streptavidin-PE, 96-well flat bottom plate,sealing tape, instructions
171304515
Bio-Plex Pro Cell Signaling Wash Buffer, 330 ml, for 1 x 96-well assay, for use with Bio-Plex Pro cell signaling assays
only, compatible with both magnetic and vacuum separation methods* Lysate controls are recommended for singleplex sets.

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 309

Bio-Plex Assays
Bio-Plex Pro RBM Apoptosis Assays

bio-rad.com/bio-plex

Bio-Plex Pro RBM Apoptosis Assays
Apoptosis Assays
The Bio-Plex Pro RBM apoptosis assays, developed inpartnership with Myriad RBM, comprise a highly relevant
set of intracellular proteins involved in the commitment,
onset, and induction of apoptosis by the intrinsic pathway.
The assays are built on magnetic beads to enable robust
quantification of multiple proteins in cell and tissue lysates,
and are available as premixed all-in-one kits.
Key features include:
■

■

■

■

■

■

■

 nalytically validated to standards set forth by theA
Clinical Laboratory Standards Institute (CLSI)
Manufactured in accordance with GMP guidelines
Lot-to-lot correlation specification of R2 ≥ 0.9 forconsistently reproducible results
2-level quality controls with lot-specific ranges
Assay quick guide to get you started right away
Fastest available assay protocols and a convenient
all-in-one kit format
Magnetic beads for simplified plate processing

Available Bio-Plex Pro RBM Apoptosis Analytes
Panel 1Panel 2

Panel 3Bak
Bax
Lamin B
Smac

Bad
Bax / Bcl-2 dimer
Bcl-xL
Bim
Mcl-1

Active caspase-3
Bcl-xL / Bak dimer
Mcl-1 / Bak dimer
Survivin

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro RBM Apoptosis Assays
Bio-Plex Pro RBM Apoptosis Panel 1, 1 x 96-well all-in-one kit that includes premixed magnetic capture beads
171WAR1CK
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, lysate dilution (LDB),
cytosolic extraction (CEB), 10x assay), 10x streptavidin-PE, flat bottom plate, plate seals, and instructions, for thedetection of the following analytes in cell and tissue lysates: Bak, Bax, Lamin B, and Smac
171WAR2CKBio-Plex Pro RBM Apoptosis Panel 2, 1 x 96-well all-in-one kit that includes premixed magnetic capture beads
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, lysate dilution (LDB),
cytosolic extraction (CEB), 10x assay), 10x streptavidin-PE, flat bottom plate, plate seals, and instructions, for thedetection of the following analytes in cell and tissue lysates: Bad, Bax / Bcl-2 dimer, Bcl-xL, Bim, and Mcl-1
Bio-Plex Pro RBM Apoptosis Panel 3, 1 x 96-well all-in-one kit that includes premixed magnetic capture beads
171WAR3CK
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, lysate dilution (LDB),
cytosolic extraction (CEB), 10x assay), 10x streptavidin-PE, flat bottom plate, plate seals, and instructions,for the detection of the following analytes in cell and tissue lysates: active caspase-3, Bcl-xL / Bak dimer,
Mcl-1 / Bak dimer, and survivin

310 | Bio-Plex Multiplex System

1,380.00

1,540.00

1,380.00

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro RBM Kidney Toxicity Assays

bio-rad.com/bio-plex

Bio-Plex Pro RBM Kidney Toxicity Assays
Kidney Toxicity Assays
The Bio-Plex Pro RBM kidney toxicity assays, developed inpartnership with Myriad RBM, comprise a highly relevant
set of biomarkers for early detection and characterization of
kidney toxicity/injury.
The assays are built on magnetic beads to enable robust
quantification of multiple proteins in human, canine, and rat
urine samples.
Key features include:■■■ nalytically validated to standards set forth by the ClinicalA
Laboratory Standards Institute (CLSI)
Manufactured in accordance with GMP guidelines
Lot-to-lot correlation specification of R2 ≥ 0.9 forconsistently reproducible results

■

■

■

■

2-level quality controls with lot-specific ranges
Assay quick guide to get you started right away
Fastest available assay protocols and a convenient
all-in-one kit format
Magnetic beads for simplified plate processing

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro RBM Kidney Toxicity Assays
Bio-Plex Pro RBM Human Kidney Toxicity Panel 1, 1 x 96-well, includes premixed magnetic capture beads,
171ATR1CK
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for thedetection of the following analytes: calbindin, clusterin, GST-p, IL-18, KIM-1, MCP-1
171ATR2CK

Bio-Plex Pro RBM Human Kidney Toxicity Panel 2, 1 x 96-well, includes premixed magnetic capture beads,
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for thedetection of the following analytes: albumin, B2M, cystatin C, NGAL, osteopontin, TFF3

171KTR1CK

Bio-Plex Pro RBM Rat Kidney Toxicity Panel 1, 1 x 96-well, includes premixed magnetic capture beads,
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for thedetection of the following analytes: clusterin, IL-18, KIM-1, MCP-1, osteopontin

171KTR2CK

Bio-Plex Pro RBM Rat Kidney Toxicity Panel 2, 1 x 96-well, includes premixed magnetic capture beads
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for thedetection of the following analytes: B2M, calbindin, cystatin C, NGAL

171KTR3CK

Bio-Plex Pro RBM Rat Kidney Toxicity Albumin Kit, 1 x 96-well, includes premixed magnetic capture beads,
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for thedetection of albumin

In the U.S., call toll free at 1-800-424-6723

1,480.00

Bio-Plex Multiplex System | 311

Bio-Plex Assays
Bio-Plex Pro Diabetes Assays

bio-rad.com/bio-plex

Bio-Plex Pro Diabetes Assays
Diabetes Assays
The Bio-Plex Pro diabetes assays are magnetic bead–based
multiplex assays designed for enhanced assay sensitivity
and assay working ranges. These assays detect human,
non-human primate, mouse, and rat diabetes-related
biomarkers using as little as 12.5 µl of sample per well.
Key features include:
■

■

■

■

Increased productivity — measure up to 10 diabetes
and obesity markers, plus adiponectin and adipsin, in
only 3 hours
 xpanded multiplexability — mix diabetes assays withE
a host of compatible assays from our cytokine menu ption to use a magnetic wash station — simplifyO
assay workflow and improve data consistency between
experiments
 lexible ordering options — order either premixedF
all-in-one kits or singleplex sets, or customize
your assay with the Bio-Plex assay builder(bio-rad.com/assaybuilder)■■Robustness — performance tested in serum andplasma matrices
Cross-platform compatibility — can be used withall xMAP life science instruments supplied by any
Luminex partner

For More Information
Web: bio-rad.com/bio-plexprodiabetes
Request or download bulletins: 6342

Available Bio-Plex Pro Diabetes Assays
Human (compatible with non-human primate samples)*

Mouse

Rat

Adiponectin
Adipsin
C-Peptide
Ghrelin
GIP
GLP-1
Glucagon
Insulin
Leptin
PAI-1
Resistin
Visfatin

Adiponectin
Ghrelin
GIP
GLP-1
Glucagon
Insulin
Leptin
PAI-1
Resistin

Ghrelin
GLP-1
Glucagon
Leptin
PAI-1* See instruction manual for details.

312 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro Diabetes Assays

bio-rad.com/bio-plex

Ordering Information
Catalog #Description

Price, U.S. $Human Premixed All-in-One Kits (compatible with non-human primate samples)
171A7001M

171A7002M

171A7003M

Bio-Plex Pro Human Diabetes 10-Plex Panel, 1 x 96-well, includes premixed coupled magnetic beads,
detection antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE, flatbottom plate, sealing tape, standard and sample diluents for the detection of C-Peptide, ghrelin, GIP, GLP-1,
glucagon, insulin, leptin, PAI-1, resistin, and visfatin
Bio-Plex Pro Human Diabetes Adipsin and Adiponectin Assays, 1 x 96-well, includes coupled magnetic
beads, detection antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE,
flat bottom plate, sealing tape, serum-based diluent for the detection of adipsin and adiponectin
Bio-Plex Pro Human Diabetes Adiponectin Assay, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE, flat bottom plate,sealing tape, serum-based diluent for the detection of adiponectin

Bio-Plex Pro Human Diabetes Singleplex Sets, 1 x 96-Well*
171B7003M
C-Peptide Set
270.00171B7004M
Ghrelin Set
270.00171B7005M
GIP Set270.00171B7006M
GLP-1 Set
270.00171B7007M
Glucagon Set
270.00Standards
171D70001
171D70050

171B7008M
171B7009M
171B7010M
171B7011M
171B7012M

Standards
171I70001

Standards
171NZ0001

1,510.00

455.00270.00270.00270.00270.00Insulin Set
Leptin Set
PAI-1 Set
Resistin Set

Bio-Plex Pro Mouse Diabetes Standards, pkg of 1 vial, lyophilized mixture of 9 standard analytes, includes
adiponectin, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, and resistin

Bio-Plex Pro Rat Diabetes Singleplex Sets, 1 x 96-Well*
171L7001M
Ghrelin Set
171L7003M
GLP-1 Set
171L7004M
Glucagon Set

270.00270.00270.00

171L7006M
171L7007M

460.00160.007,000.00

Mouse Premixed All-in-One Kits
Bio-Plex Pro Mouse Diabetes 8-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection
171F7001M
antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE, filter plate, flatbottom plate, sealing tape, standard and sample diluents for the detection of ghrelin, GIP, GLP-1, glucagon,
insulin, leptin, PAI-1, and resistin
Bio-Plex Pro Mouse Diabetes Adiponectin Assay, 1 x 96-well, includes coupled magnetic beads,
171F7002M
detection antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE,
filter plate, flat bottom plate, sealing tape, serum-based diluent for the detection of adiponectin
171G7006M
171G7007M
171G7008M
171G7009M

880.00270.00270.00270.00270.00270.00Insulin Set
Leptin Set
PAI-1 Set
Resistin Set
Visfatin Set

 io-Plex Pro Human Diabetes Standards, pkg of 1 vial, lyophilized mixture of 12 analytes
BBio-Plex Pro Human Diabetes Standards, pkg of 50 lot-matched vials, lyophilized mixture of 12 analytes

Bio-Plex Pro Mouse Diabetes Singleplex Sets, 1 x 96-Well*
171G7002M
Ghrelin Set
270.00171G7003M
GIP Set270.00171G7004M
GLP-1 Set
270.00171G7005M
Glucagon Set
270.002,125.00

160.00Leptin Set
 AI-1 Set
P

270.00270.00 io-Plex Pro Rat Standards, pkg of 1 vial, lyophilized mixture of 31 standard analytes. Compatible withB
rat cytokine and rat diabetes assays

225.00

Reagent Kit — for use with human diabetes singleplex sets
171304090M
Bio-Plex Pro Reagent Kit III, 1 x 96-well, includes detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealingtape, for magnetic separation methods

275.00

Reagent Kit — for use with rat and mouse singleplex sets
12002798
Bio-Plex Pro Reagent Kit V, 1 x 96-well, includes detection antibody diluent HP, standard diluent, sample
diluent, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate, sealing tape, for magnetic
separation methods

275.00* Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 313

Bio-Plex Assays
Bio-Plex Pro Human Isotyping Assays

bio-rad.com/bio-plex

Bio-Plex Pro Human Isotyping Assays
Human Isotyping Assays
Bio-Plex Pro human isotyping assays are magnetic
bead–based multiplex immunoassays offering accurate
and reproducible measurements of multiple analytes
simultaneously. These assays have been developed toprovide reliable performance with the flexibility required tomeet your research needs. They incorporate several features
to enhance both quality and ease of use.
■■

Available Assays
IgG1
IgG2
IgG3
IgG4
■

 agnetic beads — simplified plate processing andM
improved reproducibility■

 onvenience — all-in-one kit format for both multiplex andCsingleplex assays

■

IgA
IgM
IgE
IgG Total

1-level quality control
 ptimization — one diluent optimized for use withO
samples, standards, and controls
Assay quick guide — to get you started right away

For More Information
Web: bio-rad.com/isotyping
Download bulletin: 6344

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Pro Human Isotyping Panel Premixed All-in-One Multiplex Kit
171A3100M
Bio-Plex Pro Human Isotyping Panel, 6-Plex, 1 x 96-well, includes coupled magnetic beads, detection
antibodies, standards, 1-level quality controls, detection antibody diluent, isotyping diluent (for use with samples,standards, and controls), assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, assay
quick guide, and product data sheet, for detection of IgG1, IgG2, IgG3, IgG4, IgA, and IgM
Bio-Plex Pro Human Isotyping All-in-One Singleplex Kits*
171A3101M
IgA Kit, 1 x 96-well
171A3104M
IgM Kit, 1 x 96-well
171A3102M
IgE Kit, 1 x 96-well
171A3103M
IgG Total Kit, 1 x 96-well

1,335.00

535.00535.00535.00655.00* Singleplex kits are specific for each assay indicated and include coupled magnetic beads, detection antibodies, standards,
1-level quality controls, detection antibody diluent, isotyping diluent (for use with samples, standards, and controls), assay buffer,
wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, assay quick guide, and product data sheet.

314 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
Bio-Plex Pro Mouse Chemokine Panelbio-rad.com/bio-plex

Bio-Plex Pro Mouse Chemokine Panel
Mouse Chemokine Panel
The Bio-Plex Pro mouse chemokine panel is a ready-to-use, 1 x 96-well kit containing premixed magnetic capture
beads and detection antibodies, standards, and buffers optimized to detect 33 biologically relevant mousechemokines and cytokines in a single well. This assay delivers superior sensitivity and sample detection using
minimal input sample volumes, thereby conserving precious mouse samples.
Benefits include:■

Sample efficiency — essential for mouse models■

Short protocol — results in 4 hours■

 lexible ordering options — order either premixed all-in-one kits or singleplex sets, or customize
Fwith your assay with the Bio-Plex assay builder (bio-rad.com/assaybuilder)
The Bio-Plex Pro mouse chemokine panel includes the following targets*:
BCA-1 / CXCL13
CTACK / CCL27
ENA-78 / CXCL5
Eotaxin / CCL11
Eotaxin-2 / CCL24
Fractalkine / CX3CL1
GM-CSF
I-309/CCL1
IFN-g
IL-1β

IL-2
IL-4
IL-6
IL-10
IL-16
IP-10 / CXCL10I-TAC / CXCL11
KC / CXCL1
MCP-1 / CCL2
MCP-3 / CCL7

MCP-5 / CCL12
MDC / CCL22
MIP-1a / CCL3
MIP-1β / CCL4
MIP-2 / CXCL2*
MIP-3a / CCL20
MIP-3β / CCL19
RANTES / CCL5SCYB16 / CXCL16
SDF-1a / CXCL12TARC / CCL17
TECK / CCL25*
TNF-a* MCP-2 / CCL8, MIP-2 / CXCL2, and TECK / CCL25 are available separately in single-plex format.
For More Information
Web: bio-rad.com/bio-plexmousechemokine

Ordering Information
Catalog #Description

12009159
12002247
12002254
12002260
12002798
12002796

Bio-Plex Pro Mouse Chemokine 31-Plex, 1 x 96-well
Bio-Plex Pro Mouse Chemokine MCP-2/CCL8 Set, 1 x 96-well
Bio-Plex Pro Mouse Chemokine MIP-2 / CXCL2 Set, 1 x 96-well
Bio-Plex Pro Mouse Chemokine TECK / CCL25 Set, 1 x 96-well
Bio-Plex Pro Reagent Kit V, 1 x 96-well
Bio-Plex Pro Mouse Chemokine Standards, 1 packIn the U.S., call toll free at 1-800-424-6723

Price, U.S. $Bio-Plex Multiplex System | 315

Bio-Plex Assays
Bio-Plex Pro Human Neutralization Antibody Assays

bio-rad.com/bio-plex

Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
Bio-Plex Pro human SARS-CoV-2 neutralization antibody
assays* allow researchers to assess the efficacy of COVID-19
vaccines or compare the effectiveness of naturally acquiredor vaccine-induced antibody responses to arising variantsof concern. The highly precise and specific assays areoffered as an 11-plex 96-well plate complete kit that includes
wild-type S1, wild-type RBD, Alpha spike 1, Beta spike 1,
Gamma RBD, Epsilon RBD, Kappa RBD, D614G spike 1,
E484K RBD, K417N RBD, and N501 RBD. The wild-type
S1 and RBD are also available as a 2-plex 96-well plate kit.
For flexibility, the variants are available as coupled beads. In
addition, the Delta RBD and Delta spike trimer are available
as 2-plex coupled beads. SARS-CoV-2 antigens are coatedon magnetic beads that bind neutralizing antibodies incompetition with a biotinylated detection ACE2 receptor.
For any new emerging variants, the Bio-Plex neutralization
antibody custom assay developer kit can be used to develop
neutralization assays. The kit comes with the Bio-Plex Pro
amine coupling kit, a vial of Bio-Plex Pro magnetic COOH
beads #27, and Bio-Plex Pro serology beads storage buffer.

Key Features
Comprehensive panel coverage — detects
neutralizing antibody response to two wild-type and
11 SARS-CoV-2 variants
■

■

■

■

■

Fast protocol — results in less than 3 hours
 igh precision and specificity — for greaterHconfidence in measuring SARS-CoV-2 specificantibodies in serum and plasma samples
Reproducibility — attain consistent results in yourexperiments with a robust magnetic bead-based
workflow ustom assay developer kit — for quick custom
C
neutralization assay development with new variant

Note: For research use only. Not for diagnostic procedures.
For More Information
Web: bio-rad.com/bio-plexSARS-CoV-2NAb
Download bulletins: 3263 and 3286

Ordering Information
Catalog #Description

Multiplex Kits
12016897
12016848

Price, U.S. $Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody 11-Plex Panel, 1 x 96-well
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel, 1 x 96-well

Variant Coupled Beads
12016868
Bio-Plex Pro SARS-CoV-2 Alpha S1 Coupled Beads
12016849
Bio-Plex Pro SARS-CoV-2 Beta S1 Coupled Beads
12016898
Bio-Plex Pro SARS-CoV-2 Gamma RBD Coupled Beads
12016838
Bio-Plex Pro SARS-CoV-2 D614G S1 Coupled Beads
12017225
Bio-Plex Pro SARS-CoV-2 Delta RBD and Spike Trimer 2-Plex Coupled Beads*
12016943
Bio-Plex Pro SARS-CoV-2 E484K RBD Coupled Beads
12016875
Bio-Plex Pro SARS-CoV-2 Epsilon RBD Coupled Beads
12016942
Bio-Plex Pro SARS-CoV-2 K417N RBD Coupled Beads
12016850
Bio-Plex Pro SARS-CoV-2 Kappa RBD Coupled Beads
12016869
Bio-Plex Pro SARS-CoV-2 N501Y RBD Coupled Beads

1,335.00

535.00535.00535.00655.00

Kit & Reagents
12017037
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Reagent Kit
12016945
Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Standard
12016944
Bio-Plex Pro Biotinylated Detection ACE2 Receptor
12016837
Bio-Plex Pro Serology Beads Storage Buffer
17007632
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit* Ready to use positive control is included

316 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
bio-rad.com/bio-plex

Bio-Plex Pro Human SARS-CoV-2 Serology Assays

Bio-Plex Pro Human SARS-CoV-2 Serology Assays
Bio-Plex Pro Human SARS-CoV-2 Serology Assays
Bio-Plex Pro human SARS-CoV-2 serology assays* provide
COVID-19 researchers sensitive and specific assays tomonitor the antibody immune response profile against
four viral protein subunits. There are three isotype-specific
4-plex panels that measure antibodies against SARS-CoV-2
nucleocapsid, receptor binding domain, spike 1, and spike
2 viral protein subunits. The isotype-specific antibodies
detected are IgA, IgG, and IgM, giving the spectrum ofantibody response after COVID-19 vaccine administration or
natural exposure to SARS-CoV-2.

Key Features
Isotype specific 4-plex panels — detects antibody
response to specific viral subunits

Stand-alone components are available, allowing the flexibility
to assay only the antibodies of interest. The viral protein
subunit–coated beads can be used by researchers studying
animal models to assay antibodies in multiple species andneed only be paired with a species-specific biotinylated
antibody for detection. Bio-Plex Pro reagent kits can be used
to further streamline assay configuration.

Note: For research use only. Not for diagnostic procedures.

■

■

■

■

Fast protocol — results in less than 3 hours
 igh sensitivity and specificity — for greaterHconfidence in measuring SARS-CoV-2 specificantibodies in serum and plasma samples
 eproducibility — attain consistent results in yourR
experiments with a robust magnetic bead-based workflow

For More Information
Web: bio-rad.com/bioplexsarscov2products
Download bulletin: 7430

Ordering Information
Catalog #Description

Multiplex Assays
12014665
Bio-Plex Pro Human IgA SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, 1 x 96-well12014634
Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, 1 x 96-well12014666
Bio-Plex Pro Human IgM SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, 1 x 96-wel
Antigen Coupled Beads
12014667
Bio-Plex Pro SARS-CoV-2 N/RBD/S1/S2 4-Plex Coupled Beads
12014773
Bio-Plex Pro SARS-CoV-2 Nucleocapsid Coupled Beads
12015406
Bio-Plex Pro SARS-CoV-2 Receptor Binding Domain Coupled Beads
12014771
Bio-Plex Pro SARS-CoV-2 Spike 1 Coupled Beads
12014772
Bio-Plex Pro SARS-CoV-2 Spike 2 Coupled Beads
Antibodies
12014669
12014668
12014670

Price, U.S. $1,335.00

535.00655.00

Bio-Plex Pro Human IgA Detection Antibody
Bio-Plex Pro Human IgG Detection Antibody
Bio-Plex Pro Human IgM Detection Antibody

Kits & Reagents
12014777
Bio-Plex Pro Human Serology Reagent Kit
12002798
Bio-Plex Pro Reagent Kit V with Flat Bottom Plate, 1 x 96-well12014775
Bio-Plex Pro Human IgA SARS-CoV-2 Positive and Negative Controls
12014774
Bio-Plex Pro Human IgG SARS-CoV-2 Positive and Negative Controls
12014776
Bio-Plex Pro Human IgM SARS-CoV-2 Positive and Negative Controls

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 317

Bio-Plex Assays
Bio-Plex Pro Human Apolipoprotein Assay Panelbio-rad.com/bio-plex

Bio-Plex Pro Human Apolipoprotein Assay Panel
Human Apolipoprotein Assay Panel
The Bio-Plex Pro human apolipoprotein panel is a sensitive,
magnetic bead–based multiplex assay that allows theaccurate measurement of ten apolipoproteins in diverse
matrices, including serum and plasma. The multiplexing
feature makes it possible to quantitate the level of multiple
apolipoproteins in a single microplate well in just 3 hours,with a sample volume as low as 10 µl.
This panel is offered in a convenient, all-in-one, 10-plex kit
format that includes assay, reagent, and buffers. Standard
diluents for serum and plasma are included, along with
two-level controls and lyophilized standards.
Benefits include:
■

■

■

■

 obust performance, including lot-to-lot consistency
Rdata in each kit
Fast assay protocol with short sample incubation times
 asy collection and management of large datasetsEwith Bio-Plex Manager and Bio-Plex Data Pro software,plus automated data optimization

Available Human Apolipoprotein Assay Panel Analytes
Apo-A1
Apo-A2
Apo-B
Apo-C1
Apo-C3
Apo-D

Apo-E
Apo-H
Clusterin / Apo-J
C-reactive protein (CRP) eproducibility and simplified washing using aRmagnetic workflow

For More Information
Web: bio-rad.com/bio-plexapolipoprotein

Ordering Information
Catalog #Description

12003081

Bio-Plex Pro Human Apolipoprotein Assay Panel, 10-Plex, 1 x 96-well, includes magnetic capture beads,
detection antibodies, vial of standards, 2-level controls, diluents, buffers, streptavidin-PE, flat bottom plate, andplate seals, for the detection of 9 human apolipoproteins and CRP

318 | Bio-Plex Multiplex System

Price, U.S. $In the U.S., call toll free at 1-800-424-6723

Bio-Plex Assays
bio-rad.com/bio-plex

Bio-Plex COOH Beads and Related Reagents

Bio-Plex COOH Beads and Related Reagents
Bio-Plex Pro Magnetic COOH Beads
Carboxylated beads that enable you to build your own
magnetic assays for use with either magnetic- or vacuumbased separation protocols during assay preparation.
The magnetic beads are available in 20 bead regions in 1 ml
vials for immediate delivery (4 ml vials are available with a30-day lead time). The Bio-Plex amine coupling kit provides
a complete set of reagents for coupling proteins to the beads.
For nucleic acid coupling, refer to the instruction manual.
■■Suitable for protein and nucleic acid multiplex assays
 ompatible with all Luminex xMAP readers, bothC
Bio-Plex Pro and Bio-Plex Pro II wash stations,and Bio-Plex handheld magnetic washers

For More Information
Web: bio-rad.com/COOH

Ordering Information
Catalog #Description

MC100xx01*
MC100xx04*

Bio-Plex Pro Magnetic COOH Beads, 1 ml
Bio-Plex Pro Magnetic COOH Beads, 4 ml

Price, U.S. $685.002,720.00* xx = bead region. Bead regions available: 26–29, 34–37, 43–46, 52–55, and 62–65.

Bio-Plex Nonmagnetic COOH Beads
Develop your own Bio-Plex suspension array assays using
these beads. The beads are internally labeled with two
fluorescent dyes. Use the Bio-Plex amine coupling kit to attachnucleic acids, antibodies, or other proteins to polystyrene beads
for multianalyte analysis of any sample in a 96-well format.
■

■

■

■

Flexible assays — quickly add or remove analytes
from your menu eady-to-use reagent kits — optimized for Bio-Plex
R
COOH beads
Complete solution — one-stop source for all yourassay needs
Compatibility — works with 200 and 3D xMAP readers

For More Information
Web: bio-rad.com/COOH

Ordering Information
Catalog #Description

1715060xx*
1716060xx*

Bio-Plex Nonmagnetic COOH Beads, 1.25 x 107 beads/ml, 1 ml
Bio-Plex Nonmagnetic COOH Beads, 16 ml

Price, U.S. $410.005,330.00* xx = bead region. Bead regions available, 11, 16, 18, 20, 24–28, 30, 31, 33, 35, 38, 42–46, 50–53, 56, and 66.

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 319

Bio-Plex Assays
Bio-Plex COOH Beads and Related Reagentsbio-rad.com/bio-plex

Bio-Plex Amine Coupling Kit
This kit was developed specifically for immobilizing
antibodies or other proteins on Bio-Plex nonmagnetic
COOH beads.
■

 ufficient reagents for 30 coupling reactions using 100 µl
Sof Bio-Plex COOH beads■

Each coupling reaction supports the analysis of 200 samples■

Tested on proteins from 6–150 kD■

For use with antibodies or other proteins

For More Information
Web: bio-rad.com/COOH

Ordering Information
Catalog #Description

Price, U.S. $Bio-Plex Amine Coupling Kit
Bio-Plex Amine Coupling Kit, 30 reactions, includes bead wash buffer, bead activation buffer,
171406001*
storage buffer, staining buffer, coupling reaction tubes
Bio-Plex Streptavidin-PE
171304501
Bio-Plex Streptavidin-PE, streptavidin-phycoerythrin for 10 x 96-well reactions

535.00625.00Note: Streptavidin-PE is a component of all reagent kits and is also available separately.
* For protein coupling only.

320 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System
Subject Index

Subject Index
Apoptosis Assays, 310
Bio-Plex 3D Multiplex System, 294
Bio-Plex 200 System, 293
Bio-Plex Amine Coupling Kit, 320
Bio-Plex Data Pro Software, 298
Bio-Plex Manager Software Conversions and Upgrades, 297
Bio-Plex Manager Software, Security Edition, 297
Bio-Plex Manager Software, Standard Edition, 296
Bio-Plex Nonmagnetic COOH Beads, 319
Bio-Plex Pro Magnetic COOH Beads, 319
Bio-Plex Pro Washing Accessories, 295
Diabetes Assays, 312
Human Apolipoprotein Assay Panel, 318
Human Cytokine, Chemokine, and Growth Factor Assays, 301
Human Inflammation Assays, 299
Human Isotyping Assays, 314
Human SARS-CoV-2 Neutralization Antibody Assays, 316
Human SARS-CoV-2 Serology Assays, 317
Kidney Toxicity Assays, 311
Mouse Chemokine Panel, 315
Mouse Cytokine, Chemokine, and Growth Factor Assays, 304
Multispecies Cell Signaling Assays, 307
Multi-Species TGF-b Assays, 307
Rat Cytokine, Chemokine, and Growth Factor Assays, 306

In the U.S., call toll free at 1-800-424-6723

Bio-Plex Multiplex System | 321

322 | Bio-Plex Multiplex System

In the U.S., call toll free at 1-800-424-6723

ELISA Reagents and Accessories
Microplate Washer and Accessories
Microplate Washer
ELISA Reagents and Kits

324
324
325

ELISA Reagents and Accessories
Washerbio-rad.com/microplatereagents

Microplate Washer and Accessories
WasherImmunoWash 1575 Microplate Washer
The ImmunoWash 1575 microplate washer makes it easy tocustomize your plate-washing protocols. It offers complete
control of needle position to perform special wash routinesand maximize wash efficiency. This washer includes a wide
choice of wash sequences to facilitate protocol creation andstorage. It’s compatible with strips and 96-well microplates
that have flat, U-, or V-bottom wells.
■■■

Programmable needle positions (horizontal and vertical)

to an accuracy of 0.1 mm for bottom washing, crosswise
aspiration, and overflow washing■

Waste bottle sensor to detect high waste liquid levels

Dispensing speed control■

Up to 75 programmable washing sequences

A plate-shaking option to help minimize bubbles andadherence of liquid to well sides

For More Information
Web: bio-rad.com/immunowash
Download bulletins: 2054 and 2135

Ordering Information
Catalog #Description

1707009ImmunoWash 1575 Microplate Washer, 110–240 V, includes bottle and tubing set, 8-port manifold,aerosol protection cover
ImmunoWash 1575 12-Channel Manifold
ImmunoWash 1575 Standard Maintenance Kit

17070211707026

324 | ELISA Reagents and Accessories

Price, U.S. $6,910.00
475.00165.00In the U.S., call toll free at 1-800-424-6723

ELISA Reagents and Accessories
bio-rad.com/microplatereagents

ELISA Reagents and Kits

ELISA Reagents and Kits
See Also

ELISA Reagents
Enzyme-Antibody Conjugates
Bio-Rad offers enzyme-antibody conjugates and
ELISA soluble substrate systems suitable for use in all
microtitration enzyme immunoassays. Antibodies areavailable conjugated to HRP or AP enzymes.
Substrate Systems
The AP substrate kit contains p-nitrophenyl phosphate(pNPP) tablets and dilution buffer concentrate toprepare 500 ml of working solution. Benefits of the AP
system include an extremely linear reaction rate and low
background values for accurate quantitation by ELISA.

Blotting-grade
conjugates: page 252.

There are two convenient substrate systems for
detection of HRP-labeled antibodies. Both the
2,2'-azino-di(3-ethylbenzothiazoline)-6-sulfonicacid (ABTS) peroxidase substrate kit and the
3,3',5,5'-tetramethylbenzidine (TMB) peroxidase
substrate kit contain liquid reagents that are
mixed just prior to use.
The single-component TMB peroxidase EIA substrate kit
makes microplate assays convenient and fast.
For More Information
Web: bio-rad.com/microplatereagents

Ordering Information
Catalog #Description

Price, U.S. $Enzyme-Antibody Conjugates
1721011Goat Anti-Mouse IgG (H + L)-HRP Conjugate, 2 ml
1721033
Goat Anti-Human IgG (g)-HRP Conjugate, 1 ml
1721019
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate, 1 ml
1721034
Rabbit Anti-Goat IgG (H + L)-HRP Conjugate, 1 ml
1721017
Rabbit Anti-Sheep IgG (H + L)-HRP Conjugate, 1 ml
1721037
Rabbit Anti-Goat IgG (H + L)-AP Conjugate, 1 ml

165.00235.0094.00
185.00170.00225.00Substrate Systems
1721063AP Substrate Kit, contains 100 pNPP tablets and 100 ml 5x concentrate diethanolamine buffer
1721064
HRP Substrate Kit, contains 180 ml ABTS, 20 ml hydrogen peroxide1721066
TMB Peroxidase EIA Substrate Kit, contains 180 ml TMB solution, 20 ml hydrogen peroxide1721067
TMB Peroxidase EIA Substrate Kit, contains 900 ml TMB solution, 100 ml hydrogen peroxide1721068
Single-Component TMB Peroxidase EIA Substrate Kit, contains 100 ml TMB solution
1721072
Single-Component TMB Peroxidase ELISA Substrate Kit, contains 1 L TMB solution

230.00175.00180.00485.0081.00
710.00

Mouse Antibody Isotyping Kit
This kit provides a convenient ELISA method for identifying theclass, subclass, and light-chain type of mouse IgGs in tissue
culture supernatant and ascites fluid. The kit allows screeningof up to 12 different antibody samples per plate. Each sample

is reacted with eight subclass-specific antisera to determine
the heavy- and light-chain types of the monoclonal antibody.
For More Information
Web: bio-rad.com/microplatereagents

Ordering Information
Catalog #Description

17220511722055

Mouse Typer Isotyping Kit, includes mouse isotyping panel, affinity purified goat anti-rabbit IgG (H + L)
Mouse Typer Isotyping Panel, includes 10 ml each ultrapure rabbit anti-mouse subclass-specific antiserum
to mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, k-chain, and l-chain

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $545.00360.00ELISA Reagents and Accessories | 325

ELISA Reagents and Accessories
Subject Index

Subject Index
ELISA Reagents, 325
ImmunoWash 1575 Microplate Washer, 324
Mouse Antibody Isotyping Kit, 325

326 | ELISA Reagents and Accessories

In the U.S., call toll free at 1-800-424-6723

Transfection
Transfection Technologies

328

Lipid Transfection Reagents

329

Electroporation Systems and Buffers

330

Biolistic Particle Delivery Systems

335

Transfection Technologies
bio-rad.com/transfection

Transfection Technologies
Introducing molecules such as DNA, RNA, mRNA, RNPs (ribonucleoprotein), and other proteins into cells is a powerful toolfor evaluating gene expression. Bio-Rad’s transfection products offer choices for gene delivery to bacterial, fungal, plant,
and animal cells. Use the guide below to select the most appropriate transfection technology for your application.

Transfection Technologies

PlantsPage

Ex Vivo

Cells orTissues(e.g., blo
Suspenod)sion Cell Cultures
Mammalian Cells — G
ene KnAdhereockdow
nt Cells
ns, Trypsinized
Adherent Cells, Attached
Organelles (chloroplasts, mitochondria)ns (in v
ivo)

Cultures

Organ

Other F
ungi
Skin or
Orga

Leaves(in situ)
Detached Leaves
Meristems (in s
itu)
Detached MeristemsGram-Positive
BacteriaGram-Negative
Bacteria
Yeast

Plant Cell Suspension Cultures
Plant Cell Adherent Cultures
Plant Cell Callus or Explants
PollenLearn More about the Technology
Web: bio-rad.com/tech/transfection

Bacteria Fungi

Animals

Lipid Transfection
•

•

•

•

•

•

•

•

•

•

•

•			•

•

•

•

°

°			•

•

•

•

•

•		•

•

•

°

•		•

•

•

329													°siLentFect lipid reagent for RNAi
TransFectin lipid reagent°°

330													°Electroporation

Gene Pulser Xcell microbial system		 •*			 °		°		 •*			 °		°

332								•

°

°
•

•

MicroPulser electroporator

333								•

•

•

•

°

•

•

•

°

•

•		 •

Gene Pulser Xcell total system

332

•*

Gene Pulser Xcell eukaryotic system

332

•*

•
•

•

Biolistic Particle Delivery
Helios gene gun system

336

•

•

•

•

PDS-1000/He system

337

•

•

•

•		•		•

•

•

•

°

°

•

°

• First choice, highly recommended.
• Recommended, but other technologies may be better suited to that application.
° Possible, but little data to support its effectiveness.
* Protoplasts required.

Lipid Transfection
page 329

Electroporation
page 330

Nucleic acidIons

Biolistic Particle Delivery
page 335

Gene

A

Lipid

DNA-coatedmicrocarrier

Transfection

SampleComplexing
Cell membrane

B

Gas

Liposome

Helios Gene Gun System Particle Delivery Method

AnalysisC

328 | Transfection

In the U.S., call toll free at 1-800-424-6723

Lipid Transfection Reagentsbio-rad.com/lipids

Lipid Transfection Reagents
Lipid transfection is the process of using lipids to enable a cell to absorb DNA from outside itself. The liposome easily
merges with the membrane of the cell since they are both composed of a phospholipid bilayer. Once the liposome andmembrane are merged the genetic material can then be released inside the cell. Some familiar aliases for lipid transfection
are lipid-mediated delivery, lipofection, and liposome-based gene transfection.
Lipid transfection has been shown to be successful in transfecting adherent and suspension cells and some primary cells(for example, mES and neuronal cells). Common applications employing lipid transfection include RNAi studies, protein
production, viral production, gene function analysis, and cell-based assays.
Lipid Transfection Reagents Selection Guide		

siLentFect Lipid Reagent

TransFectin Lipid Reagent

Description

RNAi-specific lipid

General-purpose lipid

Applications

RNAi (siRNA delivery)*

Gene expression RNAi (siRNA/shRNA delivery)

Cell lines**		

Variety of adherent and suspension cell lines, including
A549, BHK, HeLa, K562, primary fibroblasts, and Vero

Variety of adherent and suspension cell lines, including
A549, HeLa, HUVEC, MCF-7, and primary fibroblasts

Shelf life

6 months

6 months* 	siLentFect can successfully cotransfect an siRNA molecule with a plasmid DNA.
** For a more complete list of cell lines, see below (siLentFect lipid reagent) and page 330 (TransFectin lipid reagent), or go to bio-rad.com/lipids.

See AlsosiLentFect Lipid Reagent for RNAi

TC20 automated cellcounter: page 34.

Effective Gene-Specific Silencing with Low Toxicity
siLentFect lipid reagent delivers siRNA to a broad varietyof cultured mammalian cells for RNAi applications. RNAi
is a powerful technique used for the specific inhibition ofgene expression. An intrinsic cellular mechanism in mosteukaryotes, RNAi helps regulate the expression of genes
critical to cell fate determination, differentiation, survival,and defense from viral infection.
■

Supermixes for PCR
and real-time PCR:
page 378.
Real-time PCRsystems: page 369.

Effective gene-specific silencing — achieve 90–99%
gene-specific knockdown of both high- and low-abundance
genes using as little as 1 nM siRNA for certain gene targets■■

Low amounts of siRNA and lipid required — the highaffinity of siLentFect lipid reagent for siRNA allows the useof less lipid and less siRNA per experiment, decreasing the
likelihood of off-target effects, reducing cost, and minimizing
the experimental bias caused by cell stress/deathSimple, flexible protocol — easily adaptable protocol
for high-throughput applications; successfully transfectcells by adding siLentFect reagent and siRNA directly tothe culture or by adding siLentFect-siRNA complexes totrypsinized cells still in suspension■■

 otransfection capabilities — simultaneous deliveryCof siRNA and dsDNA vectors for optimization and dual
expression analysis
 orks with many cell types — 184htrt, 4T1, A549,
W
Caco-2, CHO-K1, COS-7, HEK 293, HeLa, HepG2,
HUVEC, LNCaP, MCF-7, murine EC, NIH 3T3, PC-3,
primary fibroblast, primary keratinocyte, primary ovarian,
SVEC4, VSMC

For More Information
Web: bio-rad.com/silentfect
Download bulletins: 3105, 5439, and 5894

Ordering Information
Catalog #Description

1703360
1703361
1703362

siLentFect Lipid Reagent for RNAi, 0.5 ml
siLentFect Lipid Reagent for RNAi, 1.0 ml
siLentFect Lipid Reagent for RNAi, 5 x 1.0 ml

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $230.00400.001,515.00

Transfection | 329

Electroporation Systems and Buffers
bio-rad.com/lipids

See Also
Supermixes for PCR
and real-time PCR:
page 378.
Real-time PCRsystems:
page 369.
Total RNA
extraction kits:
page 9.

TransFectin Lipid Reagent
Efficient Delivery for High Gene Expression Levels
TransFectin lipid reagent delivers nucleic acids to a broad
range of cell lines with high efficiency. Advantages of thisreagent include:■

Enables high-efficiency results — effective
transfection of both easy- and difficult-to-transfect cells

Plasmid purification
kits: page 13.
■■

Minimally affects cell viability — less cytotoxicity
than other high-efficiency products makes it appropriate
for sensitive cell lines; lower toxicity leads to healthiercells for post-transfection analysis
Simple to use — part of an easy three-step protocol;
dilute TransFectin reagent and nucleic acid in theappropriate medium, mix, incubate, and add to the cell
culture. There is no need to change the medium for mostcell types after addition of the complexes; just incubate
and assay for expression■■

are required for optimal transfection results compared toother reagents; using less lipid reduces the cost pertransfection and reduces toxicity effects

Allows flexibility in experimental conditions —efficient transfection in both the presence and absenceof serum-containing media; exceptional results are
obtained when cells are transfected at densities between
40 and 90%

Affordable — minimal amounts of TransFectin reagent

For More Information
Web: bio-rad.com/transfectin
Download bulletins: 2873 and 3197

Ordering Information
Catalog #Description

1703350
1703351
1703352

TransFectin Lipid Reagent, 0.5 ml
TransFectin Lipid Reagent, 1.0 ml
TransFectin Lipid Reagent, 5 x 1.0 ml

Price, U.S. $190.00355.001,310.00

Electroporation Systems and Buffers

Voltage, V

V0

V0
e
t

330 | Transfection

Time, ms

Exponential-decay pulse.
When a capacitor charged to a
voltage V0 is discharged into cells,
the voltage applied to the cells
decreases exponentially over time.
The time required for the initial
voltage to drop to V0 /e is referred
to as the time constant (t) and is aconvenient expression of thepulse length.

medium. Electroporation provides a valuable and effective
alternative to chemical, biological, and other physicalmethods of transfection.
For More Information
Web: bio-rad.com/electroporation
Online protocols: bio-rad.com/transfectionprotocols
V0
Voltage, V

Electroporation is a powerful, highly efficient techniquefor introducing molecules (nucleic acids, proteins,carbohydrates, dyes) and viral particles into a wide variety
of prokaryotic and eukaryotic cells. A high-intensity
electric field transiently permeabilizes the membrane,
enabling uptake of molecules from the surroundingVttTime, ms

Square-wave pulse. Truncating
the pulse from a capacitor after
discharging it into the sample
generates a square-wave pulse.
The pulse length is the time thecells are subjected to the discharge.
A slight drop in voltage occurs
with all square-wave instruments.
This drop in voltage is called thepulse droop and is measured as apercentage of the initial voltage.

In the U.S., call toll free at 1-800-424-6723

Electroporation Systems and Buffers
bio-rad.com/electroporation

A Choice of Exponential-Decay or Square-Wave Pulse
The Gene Pulser Xcell electroporation system generates
both exponential and square waveforms, allowing you tochoose the waveform and protocol that work best for yourcells. Both exponential-decay and square-wave pulses

have been used effectively for electroporation. Depending
on cell type, the shape of the wave can have a significant
effect on the transfection efficiency. Electrofusion is also
possible with the Gene Pulser system.

Electroporation Systems Selection Guide		Gene Pulser Xcell System
MicroPulser System			page 332		
page 333
Plate-based system

No

No

Cuvette-based system

Yes

Yes

Eukaryotic or prokaryotic

Both

Prokaryotic

Recommended cells
		

Mammalian, bacterial, andfungal cell lines

Bacterial, yeast, and othermicroorganisms

Chamber

ShockPod chamber

ShockPod chamber

System options

Gene Pulser Xcell total system—		Gene Pulser Xcell eukaryotic system		Gene Pulser Xcell microbial system

Gene Pulser Electroporation Buffer
The formulation of Gene Pulser electroporation buffer
simulates the natural cell environment to minimize cellmortality while ensuring highly efficient delivery of nucleic
acids. This electroporation buffer is a universal reagent
for introducing siRNA, plasmid DNA, and other molecules
into various mammalian cells, including difficult-totransfect and primary cells. The buffer is compatible withelectroporation systems, including Gene Pulser Xcell,
Gene Pulser II, and most other systems. It is compatible
with both exponential and square waveforms.
Gene Pulser electroporation buffer:■■■

 llows you to optimize multiple electroporation parameters,A
including voltage and capacitance, for each cell type
Improves transfection efficiency and cell viability
Exhibits low conductivity — compatible with cuvette or
multiwell plate formats

For More Information
Web: bio-rad.com/electroporationbuffer
Download bulletin: 5582

In the U.S., call toll free at 1-800-424-6723

Transfection | 331

Electroporation Systems and Buffers
bio-rad.com/electroporation

Ordering Information
Catalog #Description

1652676
1652677 ene Pulser Electroporation Buffer, 10 x 1.8 ml
GGene Pulser Electroporation Buffer, 30 ml

Price, U.S. $315.00410.00Gene Pulser Xcell Electroporation Systems
The Gene Pulser Xcell system is a modular electroporation
system for transfecting every cell type. The system
includes a main unit, a ShockPod cuvette chamber, andyour choice of accessory modules: the capacitanceextender (CE module) or the pulse controller (PC module).

Capacitance
Extender Module

Pulse Controller Module

Features and BenefitsUniversal electroporation — transfects all cell types,
from primary and stem cells to bacteria and yeast
■

■

■

■

■

■

 reset protocols — include the most commonPmammalian and bacterial cell types
Flexibility — choice of programs for preset protocols,
optimization protocols, manual operation, or user
protocols rotocol library — collection of electroporation
P
protocols for every cell type including primary, immortal,
and bacterial cells
 ata management — enables storage and recall ofDparameters used in the previous 100 experiments foreasy troubleshooting

Main Unit
ShockPod Cuvette
ChamberReproducibility — uses PulseTrac circuitry and arc
protection to ensure reproducibility and sample protection

Gene Pulser Xcell System Selection Guide
SystemComments

Gene Pulser Xcell
Total System

The complete electroporation system for transfection of both eukaryotic and prokaryotic cells;
includes both the CE and the PC modules.

Gene Pulser Xcell
Eukaryotic System

For the electroporation of most eukaryotic cells, including mammalian cells and plant
protoplasts; includes the CE module.

Gene Pulser Xcell
Microbial System

For the electroporation of bacteria and fungi as well as other applications where high-voltage
pulses are applied to samples of small volume; includes the PC module.

For More Information
Web: bio-rad.com/xcell
Download bulletin: 5445
Request or download bulletin: 5542

332 | Transfection

In the U.S., call toll free at 1-800-424-6723

Electroporation Systems and Buffers
bio-rad.com/electroporation

Ordering Information
Catalog #Description

1652660

 ene Pulser Xcell Total System, for mammalian and microbial cells, 100/240 V, 50/60 Hz, exponentialG
decay and square-wave delivery, includes main unit, CE module, PC module, ShockPod cuvette chamber,
15 sterile cuvettes (5 each of 0.1, 0.2, and 0.4 cm gap), cuvette rack
Gene Pulser Xcell Eukaryotic System, 100/240 V, 50/60 Hz, exponential-decay (25–3,275 µF range)and square-wave delivery, includes main unit, CE module, ShockPod cuvette chamber, 5 sterile cuvettes(0.4 cm gap), cuvette rack
Gene Pulser Xcell Microbial System, 100/240 V, 50/60 Hz, exponential-decay delivery, includes main unit,
PC module, ShockPod cuvette chamber, 10 sterile cuvettes (5 each of 0.1 and 0.2 cm gap), cuvette rack

16526611652662
Components
1652666
16526671652668
1652669
1652095

Price, U.S. $Gene Pulser Xcell Main Unit, 100/240 V, 50/60 Hz
Gene Pulser Xcell CE Module, 25–3,275 µF range controlled by main unit, includes integral leads
Gene Pulser Xcell PC Module, 50–1,000 W range controlled by main unit, includes integral leads
Gene Pulser Xcell ShockPod Cuvette Chamber, includes integral leads for connection toGene Pulser Xcell, Gene Pulser II, or MicroPulser electroporators
Gene Pulser Cuvette Rack

7,770.00

7,210.00

6,480.00

5,795.00
1,970.00
1,020.00
625.0044.00

MicroPulser Electroporator
The MicroPulser electroporator is a simple yet versatile
instrument that enables safe and reproducible transformationof bacteria, yeast, and other microorganisms. Transformation
efficiencies much higher than those obtained withchemical methods can be achieved. Unique features ofthe system include:
■

■

■

■

■

■

 ne-button pulse delivery, attached cuvette chamber,O
and rapid charge time for fast sample handling
Delivery of exponential waveform for prokaryotic cells
 onvenient preset optimized programs for commonlyCstudied bacteria and fungi rc quenching system that significantly reduces arcing,A
protecting against loss of valuable samples
Broad range of parameters for manual optimization
 igh-voltage (3,000 V) capability for improved efficiency
Hin larger-volume cuvettes■

Compact, space-saving design■

Audible and visible pulse indicators■

MicroPulser electroporator with cuvette chamber attached.
Electroporator is shown connected to the cuvette chamber.

 isplay of time constant and actual voltage deliveredDto monitor reproducibility

For More Information
Web: bio-rad.com/micropulser
Download bulletin: 2751
Request or download bulletin: 5542

Ordering Information
Catalog #Description

1652100

 icroPulser Electroporator, includes a cuvette chamber with leads, 10 sterile cuvettesM(5 each of 0.1 cm and 0.2 cm gap)

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $2,215.00

Transfection | 333

Electroporation Systems and Buffers
bio-rad.com/electroporation

Gene Pulser/MicroPulser Electroporation Cuvettes
Bio-Rad offers high-quality electroporation cuvettes
that deliver consistent pulses to your valuable samples,
ensuring reproducible results. Cuvettes are available
in three different gap widths: 0.4, 0.2, and 0.1 cm, for
optimal field strength delivery to a wide range of cell types.
Features of the cuvettes include:■Guaranteed efficiency — manufactured to precise gap
tolerances to ensure maximum electroporation efficiency
and reproducibility between experiments

Universal compatibility — can be used with	Gene Pulser Xcell, Gene Pulser II, and most otherelectroporation systems
■

■

■

■

■

■

Ensured sterility — each cuvette is assembled in acleanroom environment, washed, fitted with a snug cap,
individually wrapped, and sterilized by gamma irradiation
Sturdy construction — durable polycarbonate
withstands pulses of very high voltage
Color-coded caps and bags — easy identification ofdifferent cuvette sizes
Consistent chamber shape — seamless plastic
molding eliminates leaking and keeps the aluminum
plates parallel, which is essential for uniform sample
treatment and safety mooth electrode surface — the aluminum plates areS
subjected to an 11-step etching and cleaning process foruniform pulse delivery to the entire sample

For More Information
Web: bio-rad.com/electroporationcuvettes
Download bulletin: 1908
Request bulletin: 5542

Ordering Information
Catalog #Description

Price, U.S. $Standard Packs
1652088
Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 50
1652086
Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 50
1652089
Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 50

180.00180.00180.00Jumbo Packs*
1652091
Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 500
1652092
Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 500
1652093
Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 500

1,330.00
1,330.00
1,330.00

Mini Packs
16520811652082
1652083334 | Transfection

Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 5Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 5Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 5

15.00
15.00
15.00

In the U.S., call toll free at 1-800-424-6723

Biolistic Particle Delivery Systems
bio-rad.com/biolistics

Biolistic Particle Delivery Systems
Biolistic technology, or particle bombardment, is a direct
physical method of delivering nucleic acids into cells.
The Helios gene gun and PDS-1000/He systems use
advanced biolistic technology to transform cells in situ.
This technology can be applied to the widest rangeof targets, including cell cultures, tissues, organs,plants, animals, and bacteria as well as organelles. The
instruments use a helium pulse to accelerate high-density
gold or tungsten particles coated with nucleic acids
directly into target cells. Adjusting the pressure of thehelium enables accurate penetration through the plant cellwall or cell membrane and into the cell.
Particle Delivery Application Notes and Protocols
Bio-Rad offers detailed application notes andprotocols describing biological and bombardment
conditions for many applications. Visit us on the Web at
bio-rad.com/biolistics to download application notes
and protocols.
For More Information
Web: bio-rad.com/biolistics
Download bulletin: 5443

Gas acceleration tube

Rupture disk

Microcarrier launchassembly

Macrocarrier

Stopping screen
DNA-coated microcarriers

Target cells
Target shelf

PDS-1000/He system particle delivery method. High-pressure
helium is used to propel a macrocarrier sheet loaded with DNA-coatedgold or tungsten microcarriers toward target cells. The macrocarrieris halted after a short distance and the DNA coated microcarriers
continue traveling toward the target to penetrate the cells.

Biolistic Systems Selection Guide by Application
Factors Affecting		
Transformation
Helios Gene Gun System

PDS-1000/He System

Experimental
conditions

In situ, in vitro,in vivo, ex vivo

In vitro, ex vivo,in vivo (plants)

Target area

Small (2 cm2)

Large (40 cm2)

Pressure range

100–600 psi

450–2,200 psi

Target type				

Animals: Any tissue exposed
to barrel (skin, organs); cell,
explant, and organ culture				Plants: Field and greenhouseuse, plant cell culture, explants

Plants: Small intact plants,
plant cell culture, explants		

Yeast, bacteria, other microbes

In the U.S., call toll free at 1-800-424-6723

Animals: Cell and organ culture

Yeast, bacteria, other microbes
Organelles (chloroplasts,
mitochondria, etc.)

Transfection | 335

Biiolistic Particle Delivery Systems
bio-rad.com/biolistics

Helios Gene Gun System
The Helios gene gun is a convenient handheld device thatprovides rapid and direct gene delivery to cells in situ.
The unit uses an adjustable helium pulse to sweep DNA-,
RNA-, or biomaterial-coated gold microcarriers from theinner wall of a small plastic cartridge directly into target
cells. Cartridge “bullets” are simple to prepare using thetubing prep station.
■■■

 rovides easy-to-use, rapid, versatile gene deliveryP
independent of target cell type
Facilitates both transient and stable expression
Requires only small amounts of DNA and cells; nocarrier DNA needed■

Enables codelivery of more than one plasmid■

Allows transfer of large DNA fragments■

Targets intracellular gene delivery to many cells■

Works for both in vitro and in vivo transformation■

Delivers no extraneous genes or proteins

The Helios gene gun system includes one vial of each of
the three sizes of gold microcarriers and a cartridge kit that
includes 15 m (50') of Tefzel tubing, five cartridge collection/
storage vials, five desiccant pellets, and 0.5 g of PVP for
the sample tubing coating procedure. This is sufficientmaterial (excluding nucleic acids, spermidine, CaCl2, andabsolute ethanol) for preparing approximately 1,000 genegun samples.
For More Information
Web: bio-rad.com/helios
Download bulletins: 5443 and 5446

Ordering Information
Catalog #Description

1652431* elios Gene Gun System, 100/120 V, includes Helios gene gun kit, helium hose assembly, heliumH
regulator, tubing prep station, syringe kit, Tefzel tubing, tubing cutter, Helios gene gun optimization kit
Helios Gene Gun System, 220/240 V

1652432*

Price, U.S. $InquireInquire* Additional items required for operation of the Helios gene gun system: helium tank, grade 4.5 (99.995% pure) or higher,pressurized to the desired output pressure. Required items for tubing preparation: nitrogen tank, grade 4.8 (99.998% pure) or
higher; nitrogen regulator (#1652425 recommended for U.S. standard connections); 100% ethanol (fresh for each microcarrier
precipitation); spermidine; plasmid.

Accessories for Helios Gene Gun
Sample Preparation Accessories for Helios Gene Gun
Gold microcarriers, Tefzel tubing, cartridge collection/
storage vials, and desiccant pellets are needed for Helios
gene gun sample preparation. The gold microcarriers areavailable in 0.6, 1.0, and 1.6 µm diameters. Lengths of Tefzel
tubing (up to 76 cm or 30") are coated with the DNA- or

336 | Transfection

RNA-microcarrier complexes using the tubing prep station.
Coated tubing is cut into 1.25 cm (0.5") cartridges using thetubing cutter. Sample cartridges can be stored for later use
at 4°C in cartridge collection/storage vials with a desiccantpellet. Additional barrel liners, cartridge holders, and otheraccessories are also available.

In the U.S., call toll free at 1-800-424-6723

Biolistic Particle Delivery Systems
bio-rad.com/biolistics

Ordering Information
Catalog #Description

16524121652413

Helium Hose Assembly, with Swagelok quick-connect fittings
Helium Regulator, CGA 580 female fitting (U.S. standard), with pressure relief valve;maximum pressure 2,600 psi
Low-Pressure Helium Regulator for Helios Gene Gun, maximum pressure 400 psi
Tubing Prep Station, 100/120 V, includes tubing support cylinder, power cord, O-rings, tubing
prep unit, 12' Nalgene nitrogen regulator hose, two 3/16" barb-to-male Luer-Lok fittings, nitrogenflowmeter fitting, two 1/8" barb-to-male Luer-Lok fittings, 5/64" hex wrench, 10 ml syringe holder
Tubing Prep Station, 220/240 V
Syringe Kit, includes syringe adaptor tubing (silicone, 5', 0.104" ID x 0.192" OD), five 10 ml syringes,
syringe adaptor fitting, five 1/8" barb-to-female Luer-Lok fittings
Tubing Cutter, includes tubing cutter unit, 10 razor blades
Helios Gene Gun Optimization Kit, includes 0.25 g 0.6 µm gold microcarriers, 0.25 g 1.0 µm gold
microcarriers, 0.25 g 1.6 µm gold microcarriers, cartridge kit
Cartridge Kit, contains 0.5 g PVP (MW 360,000), 5 cartridge collection/storage vials, 5 desiccant pellets,
50' Tefzel tubing
0.6 µm Gold Microcarriers, 0.25 g
1.0 µm Gold Microcarriers, 0.25 g
1.6 µm Gold Microcarriers, 0.25 g
Nitrogen Regulator for U.S. Standard Connections
O-Rings, 5
Barrel Liner, 5Cartridge Holder, white, 5Cartridge Extractor Tool, for removal of discharged cartridge
Battery, 9 V
Tefzel Tubing, 15 m (50')
Helios Diffusion Screens, 5
Helios Gene Gun Kit, 100/120 V, includes Helios gene gun, 5 O-rings, 5 barrel liners,
5 white cartridge holders, cartridge extractor tool, 9 V battery

165241416524181652420165242116524221652424
1652440
165226216522631652264
1652425
165241616524171652426
1652435
16524361652441
1652475
1652411

Price, U.S. $925.003,490.00
3,080.00
2,815.00

2,815.00
84.00
210.002,235.00
210.00625.00625.00625.002,630.00
12.00
145.00
95.00
23.00
13.00
190.0040.00
InquirePDS-1000/He System
PDS-1000/He System
The PDS-1000/He system accelerates nucleic acid–coatedgold or tungsten microparticles (0.6–1.6 µm) to velocities
necessary to transfect cells, tissues, or organelles.
The system uses a burst of high-pressure helium gas toaccelerate a plastic macrocarrier disk carrying microparticles
toward target cells. A stopping screen retains themacrocarrier while allowing the microparticles to penetrate
the target cells. The PDS-1000/He system provides:■■

A reproducible method for transfecting intact cells in
culture, requiring little manipulation of cells
Transfection of cells with unique growth requirementsthat are not amenable to other methods of gene transfer

PDS-1000/He System

In the U.S., call toll free at 1-800-424-6723

Transfection | 337

Biolistic Particle Delivery Systems
bio-rad.com/biolistics

Accessories for the PDS-1000/He andHepta Systems
These accessories are still available for the previously
discontinued PDS-1000/He System with Hepta Adaptor.
■

 upture disks of various strengths to control the forceRof the helium shock wave■

Gold particles (microcarriers) of various diameters■

Macrocarriers■

Stopping screens■■ ptimization kit to help fine-tune the bombardmentO
conditions for your cells of interest. The kit contains
samples of the gold microcarriers and 7 rupture disks,
stopping screens, and macrocarriers for
500 bombardments ot provided: helium tank, grade 4.5 (99.995% pure)Nor higher, pressurized to 2,600 psi; vacuum source

For More Information
Web: bio-rad.com/pds1000
Download bulletins: 5443 and 5447

Ordering Information
Catalog #Description

1652257*

 DS-1000/He System, includes helium pressure regulator, solenoid, spacer rods, microcarrier launchPassembly, target shelf, 5 macrocarrier holders, tubing
Voltage Converter, for 220/240 V line voltage

1652259
Accessories
16522781652335
16523221652326
1652327
1652328
1652329
1652330
16523311652332
16523361652226
165226216522631652264

Price, U.S. $500 Optimization Kit, includes 0.25 g each of 0.6, 1.0, and 1.6 µm gold microcarriers,
100 each of 7 rupture disks, 500 macrocarriers, 500 stopping screensMacrocarriers, 500
Macrocarrier Holders, 5
450 psi Rupture Disks, 100
650 psi Rupture Disks, 100
900 psi Rupture Disks, 100
1,100 psi Rupture Disks, 100
1,350 psi Rupture Disks, 100
1,550 psi Rupture Disks, 100
1,800 psi Rupture Disks, 100
Stopping Screens, 500
Hepta Stopping Screens, 50
0.6 µm Gold Microcarriers, 0.25 g
1.0 µm Gold Microcarriers, 0.25 g
1.6 µm Gold Microcarriers, 0.25 g

Inquire1,710.00
2,450.00
625.00235.00155.00155.00155.00155.00155.00155.00155.00385.00375.00625.00625.00110.00* Additional items required for operation of the PDS-1000/He system: helium tank, grade 4.5 (99.995% pure) or higher,pressurized to 2,600 psi; vacuum source.

338 | Transfection

In the U.S., call toll free at 1-800-424-6723

Transfection
Subject Index

Subject Index
Accessories for Helios Gene Gun, 336
Accessories for PDS-1000/He Systems, 338
Gene Pulser Electroporation Buffer, 331
Gene Pulser/MicroPulser Electroporation Cuvettes, 334
Gene Pulser Xcell Electroporation Systems, 332
Helios Gene Gun System, 336
MicroPulser Electroporator, 333
PDS-1000/He Systems, 337
siLentFect Lipid Reagent for RNAi, 329
TransFectin Lipid Reagent, 330

In the U.S., call toll free at 1-800-424-6723

Transfection | 339

340 | Transfection

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR
Digital PCR
Droplet Digital PCR Systems
Droplet Digital PCR Reagents and Consumables
Droplet Digital PCR Assays and Kits

342
342
349
356

Thermal Cyclers for PCR

364

Real-Time PCR Systems

369

Real-Time PCR Instrument Validation Tool

372

Real-Time PCR Software

373

qPCR Gene Expression Assays and Arrays376

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays
Reverse Transcription ReagentsStandard PCR Reagents
PCR and Real-Time PCR Accessories

378
378
394
398
399

PCR Plate Sealer

400

PCR Plastic Consumables
Thin-Wall PCR Tubes
PCR Plates
PCR Seals

401
401
404
410

Digital PCR
Droplet Digital PCR Systems

bio-rad.com/digitalpcr

Digital PCR
Droplet Digital PCR Systems
Several areas of research depend on the ability to study target DNA sequence variations. Digital PCR technology offers
the ability to quantify molecular genetic changes. It is employed by researchers using numerous applications such as copy
number variation, mutation detection, gene expression analysis, cell-free DNA quantification, viral titer genome edit
detection, microRNA (miRNA) detection, genetically modified organism (GMO) quantification, biomarker discovery, andviral load quantification. Bio-Rad’s QX ONE, QX200 AutoDG, and QX200 Droplet Digital PCR (ddPCR) systems, combinedwith our ddPCR supermixes, ddPCR assays and kits, and reliable thermal cyclers, offer researchers an easy-to-use, highly
precise, and reproducible digital PCR package.
Learn More about the Technology
Web: bio-rad.com/tech/ddPCR

See Also
C1000 Touchthermal cycler:
page 364.
PX1 PCR
plate sealer:
page 400.

Videos
Product
Demonstration

New QX600 Droplet Digital PCR Systems
The QX600 Droplet Digital PCR (ddPCR) system delivers
the same best-in-class performance you've come to
expect from Bio-Rad's ddPCR systems, with the additionof more channels for multiplexing. The system provides
precise and sensitive multiplexing of more targets per
sample with accurate and reproducible quantification. The
QX600 ddPCR system includes the new QX600 droplet
reader, enabling six-color detection for multiplexing,
allowing the clear discrimination of multiple targets withassays that can port over from the QX200 ddPCR system.
■

bio-rad.com/
ddpcr-qx100■

Customer
Testimonial■

bio-rad.com/
ddpcr-biomarkers■

Customer
Testimonial oost multiplexing capability with six-color detection andBadvanced multiplexing strategies

QX600 Droplet Reader chieve precise and sensitive multiplexing in multipleA
applications such as rare mutation detection, gene
expression analysis, copy number variation studies,and research into infectious diseases
 e confident in accurate and reproducible results —B
the system has been qualified by traceable material
from the National Measurement Institute, Australia
 asy to transfer from the QX200 system with the sameE
reagents, consumables, and assays

For More Information
Web: bio-rad.com/qx600

QX600 Droplet
Digital PCR Systems

bio-rad.com/
ddpcr-leukemia

Product
Demonstration

bio-rad.com/
ddpcr-qx200

342 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Digital PCR
bio-rad.com/digitalpcr

Droplet Digital PCR Systems

Ordering Information
Catalog #Description

QX600 Droplet Digital PCR Systems
17007769
QX600 Droplet Digital PCR System, includes QX600 droplet reader, ddPCR computer, and QX200
droplet generator
17008371
QX600 AutoDG Droplet Digital PCR System, includes QX600 droplet reader, ddPCR
computer, and automated droplet generator
12013328
QX600 Droplet Reader, includes droplet reader, 2 plate holders, 1 USB cable, 1 power cord
1864002
QX200 Droplet Generator, includes droplet generator, 1 box of 24 cartridges, 1 pkg of 24 gaskets, 2 cartridge
holders, 1 power cord
1864101
Automated Droplet Generator, includes automated droplet generator, 1 power cord
Consumables and Accessories for QX600 Droplet Digital PCR System
1863004
ddPCR Droplet Reader Oil, 2 x 1 L bottles
1863005
Droplet Generation Oil for Probes, 10 x 7 ml bottles
1864008
DG8 Cartridges for QX200 Droplet Generator, 1 pkg of 24 cartridges
1863009
DG8 Gaskets for QX200 Droplet Generator, 1 pkg of 24 gaskets
1864007
Droplet Generator Cartridges and Gaskets, 5 pkg of 24 DG8 cartridges, 5 pkg of 24 DG8 gaskets
1863051
DG8 Cartridge Holder
12001925
ddPCR 96-Well Plates, pkg of 25, clear shell, clear well, semi-skirted plates, for use with QX600/QX200 Droplet
Digital or AutoDG Droplet Digital PCR systems
1814040
Pierceable Foil Heat Seal, package of 100
1864005
Droplet Generation Oil for EvaGreen, 2 x 7 ml bottles
1864006
Droplet Generation Oil for EvaGreen, 10 x 7 ml bottles
12018108QX Manager Software, Premium Edition, control and analysis software with support for locked assay protocols
and tools for 21 CFR Part 11 compliance
12017322
QX Designer, tool that gives customers the ability to create, edit and deploy Assay Protocol Files (APF) for use
with QX Manager v2.0 Premium Edition
Consumables and Accessories for Automated Droplet Generator Only
1864108
DG32 Automated Droplet Generator Cartridges, 1 pkg of 30, enough for 10 x 96-well ddPCR plates
1864109
DG32 Automated Droplet Generator Cartridges, 1 pkg of 60, enough for 20 x 96-well ddPCR plates
1864110
Automated Droplet Generation Oil for Probes, 140 ml, enough for 20 x 96-well ddPCR plates
1864112
Automated Droplet Generation Oil for EvaGreen, 140 ml, enough for 20 x 96-well ddPCR plates
1864120
Pipet Tips for AutoDG System, 1 pkg of 20, enough for 10 x 96-well ddPCR plates
1864121
Pipet Tips for AutoDG System, 1 pkg of 40, enough for 20 x 96-well ddPCR plates
1864125
Pipet Tip Waste Bins for AutoDG System, 1 pkg of 10, enough for 10 x 96-well ddPCR plates

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 343

Digital PCR
Droplet Digital PCR Systems

bio-rad.com/digitalpcr

QX ONE Droplet Digital PCR System
The QX ONE Droplet Digital PCR (ddPCR) system expands
upon the most proven digital PCR technology for unmatchedprecision and sensitivity in absolute quantification withadvanced multiplex capabilities, high throughput, and fully
integrated operation. The QX ONE ddPCR system takes careof droplet generation, thermal cycling, and reading so thatyou don’t have to.
■

■

■

■

■

 oost ddPCR capacity with high throughput, automated,Bwalk-away operation.
 implified workflows with fully integrated instrument andS
consumable with droplet generation, cycling, and reading
in a single plate.
 fficient assays with advanced 4-color multiplexing andEsoftware supporting amplitude multiplexing, probe mixing,
and drop-off assay strategies.
 eamless integration with existing Bio-Rad ddPCR
S
supermixes, probe chemisty assays, and kits.
 treamlined compliance via installation qualification (IQ),
S
operational qualification (OQ), performance qualification(PQ) and regulatory edition software for U.S. FDA 21 CFRPart 11 compliant operation■

 ast and flexible cell and gene therapy assay
Fdevelopment with validated, predesigned assays andkits, and/or custom assay design with the cell and genetherapy design engine.

For More Information
Web: bio-rad.com/QXONE

Ordering Information
Catalog #Description

Price, U.S. $QX ONE Droplet Digital PCR System
12006536
QX ONE Droplet Digital PCR System, fully integrated, 4-color, high-throughput Droplet Digital PCR platform
17005930
QX ONE Droplet Digital PCR System with PX1 PCR Plate Sealer, includes QX ONE Droplet Digital PCR
instrument, touch screen, QX ONE accessory kit, and PX1 PCR plate sealer
Consumables and Accessories
12006057
QX ONE Droplet Reader Oil, for use with the QX ONE ddPCR system, enough for 10 ddPCR plates
12006058
QX ONE Droplet Generation Oil for Probes, for use with the QX ONE ddPCR system, enough for
18 ddPCR plates
12006858
GCR96 Cartridge, for use with QX ONE ddPCR system, 96 reactions
12006859
GCR96 Cartridges, for use with QX ONE ddPCR system, 96 reactions, pkg of 10
12006060
QX ONE Droplet Reader Waste Bottle, extra bottle for collection of system waste
12006843
GCR96 Foil Heat Seal, foil heat seal for GCR96 cartridge
12012078
QX ONE Software, Regulatory Edition, QX ONE system control and analysis software with traceability
for secure environments

344 | DNA Amplification/PCR

InquireInquireInquireIn the U.S., call toll free at 1-800-424-6723

Digital PCR
Droplet Digital PCR Systems

bio-rad.com/digitalpcr

QX200 Droplet Digital PCR Systems
The QX200 Droplet Digital PCR (ddPCR) system provides
an absolute quantification of target DNA or RNA moleculeswith unmatched precision and sensitivity for digital PCRapplications.
Benefits
Unmatched precision and sensitivity in multiple
applications by droplet partitioning technology reducing
bias from amplification and PCR inhibitors
■

■

■

■

■

■

Simplified absolute quantification without standard curve lexible system configuration with QX200 dropletF
generator and automated droplet generator
 lexible digital PCR chemistry — optimized for TaqMan
Fhydrolysis probes and EvaGreen® dye assay
 lexible assay setup — scalable for high sensitivity orFhigh throughput
 igh throughput and easy-to-use workflow withH
96-sample throughput

Applications
Mutation detection — superior sensitivity for detectionof rare single nucleotide polymorphisms (SNPs),
allelic variants, and circulating DNA in tissue andliquid biopsy samples
■

■

■

■

■

■

■

■

 opy number variation — high precision and resolutionC
to discriminate between small changes in copy number
and distinguish changes in a complex background enome editing — detection of rare genome editingG
events and distinction between homology-directed
repair (HDR) and non-homologous end joining (NHEJ)
DNA repair mechanisms
 athogen detection — quantification of low-abundancePmicroorganisms in complex backgrounds, monitoring of
microbial drug resistance, measurement of viral loads,
and microbiome analysis

The QX200 droplet generator partitions samples containing
genomic DNA, cDNA, or RNA into ~20,000 nanoliter-sized
droplets (8 samples/run). After PCR using a Bio-Rad thermal
cycler, droplets from each sample are streamed in single
file through the QX200 droplet reader. The PCR-positive
and PCR-negative droplets are counted to provide absolute
quantification of target DNA in digital form (96 samples/run).
Accessories
Droplet generation and reader oils, ddPCR reagents, droplet
generator cartridges, and gaskets are used with the system.
Automated Droplet Generator
The automated droplet generator provides hands-free
droplet generation for up to 96 samples quickly andreproducibly.
■ rovides a high-throughput solution for digital PCR
P
with the QX200 AutoDG ddPCR system■

Minimizes setup time■

Eliminates user-to-user variability■

Reduces contamination risk during droplet generation

 ene expression analysis — reliably measure low levels
Gof mRNA and miRNA without using a standard curve ext-generation sequencing (NGS) — quantify NGS
Nlibraries without the use of standard curves; validate
NGS results
 nvironmental — popular for quality testing in a wideEvariety of environmental samples such as soil and water ood testing — validated method for routine evaluationFof GMOsIn the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 345

Digital PCR
Droplet Digital PCR Systems

bio-rad.com/digitalpcr

Droplet Digital PCR Workflow
1

2

3

4

Prepare ddPCR
reaction mix withhydrolysis probes
or EvaGreen®

Generate
droplets

Perform PCR

Read andanalyze
results

1

For More Information
Web: bio-rad.com/digitalpcr

2

3 4 5 6 7
DG8 Cartridge

8

Ordering Information
Catalog #Description

Price, U.S. $QX200 Droplet Digital PCR Systems
1864001
QX200 Droplet Digital PCR System, includes QX200 droplet reader, ddPCR computer, and QX200
droplet generator
1864100
QX200 AutoDG Droplet Digital PCR System, includes QX200 droplet reader, ddPCR
computer, and automated droplet generator
1864003
QX200 Droplet Reader, includes droplet reader, 2 plate holders, 1 USB cable, 1 power cord
1864002
QX200 Droplet Generator, includes droplet generator, 1 box of 24 cartridges, 1 pkg of 24 gaskets, 2 cartridge
holders, 1 power cord
1864101
Automated Droplet Generator, includes automated droplet generator, 1 power cord
Consumables and Accessories for QX200 ddPCR System
1863004
ddPCR Droplet Reader Oil, 2 x 1 L bottles
1863005
Droplet Generation Oil for Probes, 10 x 7 ml bottles
1864008
DG8 Cartridges for QX200 Droplet Generator, 1 pkg of 24 cartridges
1863009
DG8 Gaskets for QX200 Droplet Generator, 1 pkg of 24 gaskets
1864007
Droplet Generator Cartridges and Gaskets, 5 pkg of 24 DG8 cartridges, 5 pkg of 24 DG8 gaskets
1863051
DG8 Cartridge Holder
12006834
Droplet Reader Plate Holder
12001925
ddPCR 96-Well Plates, pkg of 25, clear shell, clear well, semi-skirted plates
1814040
Pierceable Foil Heat Seal, package of 100
1864005
Droplet Generation Oil for EvaGreen, 2 x 7 ml bottles
1864006
Droplet Generation Oil for EvaGreen, 10 x 7 ml bottles
12018108QX Manager Software, Premium Edition, control and analysis software with support for locked assay protocols
and tools for 21 CFR Part 11 compliance
Consumables and Accessories for Automated Droplet Generator Only
1864108
DG32 Automated Droplet Generator Cartridges, 1 pkg of 30, enough for 10 x 96-well ddPCR plates
1864109
DG32 Automated Droplet Generator Cartridges, 1 pkg of 60, enough for 20 x 96-well ddPCR plates
1864110
Automated Droplet Generation Oil for Probes, 140 ml, enough for 20 x 96-well ddPCR plates
1864112
Automated Droplet Generation Oil for EvaGreen, 140 ml, enough for 20 x 96-well ddPCR plates
1864120
Pipet Tips for AutoDG System, 1 pkg of 20, enough for 10 x 96-well ddPCR plates
1864121
Pipet Tips for AutoDG System, 1 pkg of 40, enough for 20 x 96-well ddPCR plates
1864125
Pipet Tip Waste Bins for AutoDG System, 1 pkg of 10, enough for 10 x 96-well ddPCR plates

346 | DNA Amplification/PCR

InquireInquireInquireInquireInquire1,240.00
270.00225.0058.00
1,375.00
545.00545.0058.00
270.001,460.00
2,600.00
575.00575.00335.00625.0031.00

In the U.S., call toll free at 1-800-424-6723

Digital PCR
Droplet Digital PCR Systems

bio-rad.com/digitalpcr

New QX Manager Software
QX Manager is a powerful, user friendly software package
for the acquisition and analysis of Droplet Digital PCR datawhen connected to a QX200 or QX600 droplet reader.
Contact your representative if you need information about
previous versions of software. For the latest software
versions and the most up-to-date information, please visit
the listed product web pages.
QX Manager Software, Standard Edition
Turn your ddPCR experiments into data rich results withjust a few clicks. With QX Manager software, Standard
Edition, you can quickly and easily set up your plateexperiments for acquisition with a connected QX200 or
QX600 droplet reader and produce result data that canbe analyzed with its powerful, built in tools. QX Manager
software, Standard Edition supports a variety of experimentand assay types and features an array of visualization toolsthat can be readily exported for publications, posters andweb use.
With QX Manager software, Standard Edition you can:
■

■

■

■

■

 et up ddPCR experiments with up to 12 targets whenS
connected to a QX600 droplet reader

QX Manager Software, Premium Edition
QX Manager software, Premium Edition features all of thetools and functionality found in QX Manager software,Standard Edition, with the addition of support for locked,
end-to-end Assay Protocol Files and tools for 21 CFRPart 11 compliance in secure environments. QX Manager
software, Premium Edition gives you the ability to use
locked Assay Protocol Files to ensure consistent plate
configuration, perform custom calculations, as well as
displaying results tailored to your specification. Additionally,
QX Manager software, Premium Edition produces securedata files and features a robust audit trail to preserve theintegrity and validity of your ddPCR data.
With QX Manager software, Premium Edition, you can:
■

■

■

■

 reate customized templates for plates and reports,
C
enabling standardization of data acquisition and reporting iew ddPCR experiment data as it is being acquired,Vusing the Live Analysis function during the droplet
reading process nalyze your data files using a variety of chartsAand tables, including 1D Amplitude, 2D Amplitude,
Concentration, and Ratio charts■

 nsure that plates are configured in a consistent mannerEwith Assay Protocol Files erform custom calculations on your ddPCR data using
P
Assay Protocol Files iew your ddPCR data using a tailored display layoutVdefined by Assay Protocol Files rack modifications made to your ddPCR data with aTcomprehensive audit trail that includes the date and timeof the change, and user who made the change anage system access with user permissions that canM
be assigned in any combination

For More Information
Web: bio-rad.com/qxsoftware

 xport your charts, tables, and droplet amplitude dataEwith just a few clicks

QX Manager System Requirements
Operating system
CPU
Hard drive space:

Windows 10 64-bit
Minimum: 6th Generation Intel 2 Core Processor,
Recommended: 8th Generation Intel 4 Core Processor
Minimum: 500 GB, Recommended: 1 TB

System memory

Minimum: 8 GB, Recommended: 32 GB

Display resolution

Minimum: 1920 x 1080

Ports

1 USB

Drive

USB 2.0 Hi-Speed or above

Ordering Information
Catalog #Description

12018108QX Manager Software, Premium Edition, includes 5 user licenses, USB installation drive
NOTE: QX Manager software, Standard Edition does not have a part number as it is download-only anddoes not have a physical part associated with it.

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 347

Digital PCR
Droplet Digital PCR Systems

bio-rad.com/digitalpcr

New QX Designer Software
QX Designer is a software package that gives you theability to create fully customized and locked assay
protocols that can be integrated into your ddPCR workflow
with QX Manager software, Premium Edition. Assay
protocols ensure consistency across plate runs throughstandardized plate layouts, automated analysis, andcustom result data display.
With QX Designer software you can:■ nsure consistency across plate runs by defining rules forEplate configuration

■

■

■

 erform customized calculations of your own design,Putilizing data from one or more wells
 educe operator variability with auto-thresholdingR
algorithms, including support for external algorithms
tailored to your specific assay needs isplay results that are customized to your assay
DprotocolFor More Information
Web: bio-rad.com/qxdesigner

Ordering Information
Catalog #Description

12017322

QX Designer Software, Assay Protocol File creation and editing software for use with QX Manager
software, Premium Edition348 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Digital PCR
bio-rad.com/digitalpcr

Droplet Digital PCR Reagents and Consumables

Droplet Digital PCR Reagents and Consumables
Bio-Rad offers PCR reagents for exclusive use with the Droplet Digital PCR (ddPCR) systems. These reagents include
supermixes, consumables, and assays for a range of digital PCR applications.

ddPCR Supermix for Probes (No dUTP)
ddPCR supermix for probes (no dUTP) is a ready-to-use
2x supermix containing all components except primers
and template. The supermix has been optimized to deliver
maximum PCR efficiency, specificity, and sensitivity.
■

Limits nonspecific PCR amplification■

Allows for DNA recovery after amplification■

Recommended for use with ddPCR assays

For More Information
Web: bio-rad.com/digitalpcr
Request or download bulletin: 6338

Ordering Information
Catalog #Description

1863023

 dPCR Supermix for Probes (No dUTP), 2 x 1 ml vials, 2x supermix, for use with QX600,
d
QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems
ddPCR Supermix for Probes (No dUTP), 5 x 1 ml vials, 2x supermix, for use with QX600,
QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems
ddPCR Supermix for Probes (No dUTP), 5 x 5 ml vials, 2x supermix, for use with QX600,
QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems

1863024
1863025

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $175.00440.002,180.00

DNA Amplification/PCR | 349

Digital PCR
Droplet Digital PCR Reagents and Consumables

bio-rad.com/digitalpcr

ddPCR Multiplex Supermix
The ddPCR multiplex supermix is a 4x supermix
specifically designed and optimized for multiplexing.

■Optimized for multiplex assays■

Allows for more sample input■

Limits nonspecific PCR amplification

For More Information
Web: bio-rad.com/digitalpcr

Ordering Information
Catalog #Description

12005909

 dPCR Multiplex Supermix, 2 x 0.6 ml vials, 4x supermix, for use with QX600, QX600 AutoDG, QX200,d
QX200 AutoDG, and QX ONE Droplet Digital PCR systems
ddPCR Multiplex Supermix, 5 x 0.6 ml vials, 4x supermix, for use with QX600, QX600 AutoDG, QX200,
QX200 AutoDG, and QX ONE Droplet Digital PCR systems
ddPCR Multiplex Supermix, 5 x 2.5 ml vials, 4x supermix, for use with QX600, QX600 AutoDG, QX200,
QX200 AutoDG, and QX ONE Droplet Digital PCR systems

12005910
12005911

Price, U.S. $175.00440.002,180.00

ddPCR Supermix for Probes
ddPCR supermix for probes is a ready-to-use 2x supermix
used to partition and amplify DNA for digital PCR. It is
suitable for use with UNG decontamination protocols,
which prevent the reamplification of carryover PCR
products between experiments.
■■

Ensures precise target quantification

 ses standard cycling protocols for probe-basedUsimplex or duplex Droplet Digital PCR

For More Information
Web: bio-rad.com/digitalpcr
Request or download bulletin: 6338

Ordering Information
Catalog #Description

1863026

ddPCR Supermix for Probes, 2 x 1 ml vials, 2x supermix, for use with QX600, QX600
AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems
ddPCR Supermix for Probes, 5 x 1 ml vials, 2x supermix, for use with QX600, QX600
AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems
ddPCR Supermix for Probes, 5 x 5 ml vials, 2x supermix, for use with QX600, QX600
AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems

18630101863027

350 | DNA Amplification/PCR

Price, U.S. $175.00440.002,180.00

In the U.S., call toll free at 1-800-424-6723

Digital PCR
bio-rad.com/digitalpcr

Droplet Digital PCR Reagents and Consumables

See Also

ddPCR Supermix for Residual DNA Quantification

C1000 Touchthermal cycler:
page 364.

ddPCR supermix for residual DNA quantification is a
2x concentrated, ready-to-use digital PCR master mix
optimized to deliver maximum PCR efficiency, specificity,and sensitivity for direct quantification of residual hostcell DNA with the QX200, QX200 AutoDG, and QX ONE
Droplet Digital PCR (ddPCR) systems.
■

■

■

■

■

■

No DNA purification requiredFree of detectable Escherichia coli, Chinese hamster
ovary (CHO), mouse, human, and yeast DNA
Contains all components required for hydrolysis
probe–based ddPCR assays, except primers, probe(s),and template
Limits nonspecific PCR amplification
Includes hot-start polymerase to keep enzyme inactive
during sample droplet partitioningProvides absolute quantification of residual host cellDNA, which is critical for environmental monitoring,food testing, etc

PX1 PCR
plate sealer:
page 400.

For More Information
Web: bio-rad.com/digitalpcr

Ordering Information
Catalog #Description

1864037

ddPCR Supermix for Residual DNA Quantification, 2 x 1 ml vials, 2x supermix, for use in residual DNA detection with
the QX600, QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems 350.00
ddPCR Supermix for Residual DNA Quantification, 5 x 1 ml vials, 2x supermix, for use in residual DNA detection with
the QX600, QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems 85.00
ddPCR Supermix for Residual DNA Quantification, 5 x 5 ml vials, 2x supermix, for use in residual DNA detection with
the QX600, QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems0

1864038
1864039

Price, U.S. $One-Step RT-ddPCR Advanced Kit for Probes
One-step RT-ddPCR advanced kit for probes is a reverse
transcription digital PCR supermix delivering improved
efficiency, specificity, and sensitivity for precise RNA target
quantification. It contains thermostable enzymes thatallow for RNA templates to be reverse transcribed andsubsequently amplified in the same reaction tube.
■■

 ptimized enzyme blend enables partitioning of RNA
O
samples into droplets while keeping enzymes inactive
until reverse transcription at 50°C
 ontains RNase inhibitor that protects the RNA
C
throughout the workflow

For More Information
Web: bio-rad.com/digitalpcr
Download bulletin: 6250

Ordering Information
Catalog #Description

1864021

One-Step RT-ddPCR Advanced Kit for Probes, 2 x 1 ml, 200 x 20 µl reactions, for use with
QX600, QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems
One-Step RT-ddPCR Advanced Kit for Probes, 5 x 1 ml, 500 x 20 µl reactions , for use with
QX600, QX600 AutoDG, QX200, QX200 AutoDG, and QX ONE Droplet Digital PCR systems

1864022In the U.S., call toll free at 1-800-424-6723

Price, U.S. $980.002,450.00

DNA Amplification/PCR | 351

Digital PCR
Droplet Digital PCR Reagents and Consumables

bio-rad.com/digitalpcr

QX200 ddPCR EvaGreen® Supermix
The QX200 ddPCR EvaGreen® supermix is aready-to-use 2x supermix containing a dsDNA
binding dye. The supermix supports dsDNA detection
following amplification using commercially available
primer assays.
■■

 ecommended for use with Bio-Rad’s ddPCR geneR
expression assays
 ompatible with QX600, QX600 AutoDG, QX200, andC
QX200 AutoDG Droplet Digital PCR systems

For More Information
Web: bio-rad.com/digitalpcr
Download bulletin: 6473

Ordering Information
Catalog #Description

1864033

QX200 ddPCR EvaGreen Supermix, 2 x 1 ml vials, for use with QX600, QX600 AutoDG, QX200,
and QX200 AutoDG Droplet Digital PCR systems
QX200 ddPCR EvaGreen Supermix, 5 x 1 ml vials , for use with QX600, QX600 AutoDG, QX200,
and QX200 AutoDG Droplet Digital PCR systems
QX200 ddPCR EvaGreen Supermix, 5 x 5 ml vials , for use with QX600, QX600 AutoDG,
QX200, and QX200 AutoDG Droplet Digital PCR systems

1864034
1864035

Price, U.S. $175.00450.002,180.00

ddPCR 96-Well Plates
Designed and validated for Bio-Rad’s Droplet Digital
PCR workflow, ddPCR 96-well plates are for use with the
QX600, QX600 AutoDG, QX200, and QX200 AutoDG
Droplet Digital PCR systems. These 96-well semiskirted PCR plates are optimized for maximum ddPCR
performance.
■

■

■

■

 ompatible with the QX600, QX600 AutoDG, QX200,
C
and QX200 AutoDG Droplet Digital PCR systems
Compatible with the ddPCR residual DNA
quantification workflow
Free of detectable DNase and RNase
Validated for use with pierceable foil heat seals
and the PX1 PCR plate sealer

For More Information
Web: bio-rad.com/ddPCRplates
Download bulletin: 10000060943

Ordering Information
Catalog #Description

12001925

ddPCR 96-Well Plates, pkg of 25, clear shell, clear well, semi-skirted plates, for use with QX600, QX600 AutoDG,
QX200, and QX200 AutoDG Droplet Digital PCR systems

352 | DNA Amplification/PCR

Price, U.S. $In the U.S., call toll free at 1-800-424-6723

Digital PCR
bio-rad.com/digitalpcr

Droplet Digital PCR Reagents and Consumables

SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit
The Bio-Rad SARS-CoV-2 ddPCR kit is a powerful
diagnostic tool in the battle against COVID-19. Thispartition-based endpoint real-time PCR test is intended forthe qualitative detection of nucleic acids from SARS-CoV-2
in nasopharyngeal anterior nasal and mid-turbinate nasal
swab specimens, as well as nasopharyngeal wash/aspirate
and nasal aspirate specimens from patients suspected ofhaving COVID-19 by their healthcare providers. The U.S.
Food and Drug Administration (FDA) granted emergency
use authorization (EUA) for the Bio-Rad SARS-CoV-2
ddPCR kit in the U.S. on May 1, 2020.
For More Information
Web: bio-rad.com/SARS-CoV2ddPCRKit
Download bulletin: 7372

Ordering Information
Catalog #Description

12013743

SARS-CoV-2 ddPCR Kit, 600 tests per kit, includes 2019-nCoV CDC ddPCR triplex assay, one-step
RT-ddPCR supermix, reverse transcriptase, DTT, positive controls, and negative controls

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $2,180.00

DNA Amplification/PCR | 353

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

Validated ddPCR
Assay Kits andMultiplexes

ddPCR Assays
Designed Instantly
OnlineThe digital assay site allows Droplet Digital PCR(ddPCR) users to find and buy assays across a variety
of applications. The digital assay site can be accessed
directly at bio-rad.com/digital-assays.

354 | DNA Amplification/PCR

Validated Menu
of ddPCR Assays

Search curated lists of pre-designed assays, wet-lab
validated assays, or design and customize your own
assay employing one of the assay design engines. Built
by ddPCR experts, the design engines consider GC
content, melting temperature, primer-probe placement,
and genome specificity to customize a ready-to-use assay
optimized for ddPCR.

In the U.S., call toll free at 1-800-424-6723

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

Reliable Results with Pre-Designed and Validated
ddPCR Assays
■

■

■


Searchfor an assay by gene name, COSMIC ID,
or chromosomal location
 uickly search curated lists of expertly designed
Q
assays vailable in multiple fluorophore combinations andAreaction sizes to support your ddPCR instruments

In the U.S., call toll free at 1-800-424-6723

Save Time with ddPCR Design Engines andCustom Assays■

 et a ddPCR-optimized assay for your targetG
sequence, gene, or mutation■

Customize fluorophore and reaction sizes■

Save your assays and order anytime

For More Information
Web: bio-rad.com/digitalassays
Download bulletin: 6901

DNA Amplification/PCR | 355

Digital PCR
Droplet Digital PCR Assays and Kits

www.bio-rad.com/digitalpcr

Droplet Digital PCR Assays and Kits
See AlsoqPCR
Gene Expression
assays and arrays:
page 376.

Droplet Digital PCR Assays
Bio-Rad offers a comprehensive assay portfolio for
digital PCR, including:
ddPCR Gene Expression Assays

ddPCR Gene Expression assays enable precise
quantification of <2-fold changes in gene expression.
They are orderable through the Gene Expression
design engine (bio-rad.com/digital-assays/assayscreate/gene-expression).
■

■

■

■

Ideal for detection of low expressing genes and samples
that may contain PCR inhibitors

ddPCR Copy Number Determination Assays
ddPCR Copy Number Determination assays offer highprecision and resolution to discriminate between smallchanges in copy number, such as a 1.2 fold change from
5 to 6 copies..
■■■

 ver 60,000 gene expression assays for human, rat,O
and mouseSuitable for use with one- or two-step RT-ddPCR protocols
 vailable in multiple probe fluorophores or as primer onlyA
assays for use with EvaGreen® chemistry

For More Information
Web: bio-rad.com/ddPCRGeneExpressionAssays

ddPCR Mutation Detection Assays

ddPCR Mutation Detection assays offer superiorsensitivity for detection of rare single nucleotide
polymorphisms (SNPs) and allelic variants.

■

■

■

■

■

■

 n-demand assay design is available for MutationO
Detection assays through the Mutation Detection design
engine (bio-rad.com/digital-assays/assays-create/mutation)■ rderable in convenient single-tube format (mutant andOwild-type assays in a single tube) or in individual tubes

 n-demand assay design is available for copy numberO
determination through the Copy Number Determination
design engine (bio-rad.com/digital-assays/assayscreate/cnd)
Available in multiple fluorophores and reaction sizes

ddPCR Genome Edit Detection Assays
ddPCR Genome Edit Detection assays enable detection ofhomology-directed repair (HDR) and non-homologous end
joining (NHEJ) events in user-specified sequences, allowing
rapid assessment of HDR and NHEJ edits generated by
CRISPR-Cas9 or other genome editing tools.

 et-lab validated assays are available for more than 200
Wtargets. Validation data are available online

 uitable for use with circulating tumor DNA, liquid biopsy,
Sand formalin-fixed, paraffin-embedded (FFPE) and othertissue samples

 et-lab validated centromeric reference assays areWavailable for ~60 targets

For More Information
Web: bio-rad.com/ddPCRCopyNumberDeterminationAssays■■

 et-lab validated assays are available for more than 700
Wtargets. Validation data are available online■■

Detection of editing events present at frequencies ≤0.5%
 bsolute quantification of genome editing events from asAlittle as 5 ng of total genomic DNA (cells from a single wellof a 96-well plate) ssays distinguish between homozygous andAheterozygous edits in clonal populations
 esults are easily analyzable using Drop Off Analysis
Rmode in QX Manager and QX ONE softwareAvailable in multiple fluorophores and reaction sizes

For More Information
Web: bio-rad.com/ddPCRGenomeEditDetectionAssays

Available in multiple fluorophores and reaction sizes

For More Information
Web: bio-rad.com/ddPCRMutationDetectionAssays

356 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

ddPCR Cell and Gene Therapy Assays
ddPCR Cell and Gene Therapy (CGT) assays include two
sets of assays — vector backbone assays and gene ofinterest assays — that enable precise and robust viral titermeasurements in a sample. They are orderable throughthe Cell and Gene Therapy design engine (bio-rad.com/
digital-assays/assays-create/cell-and-gene-therapy).
■In-silico multiplexing checks to enable two-dimensional
viral titer measurements and genome integrity analysis■

Assay sequence provided with select catalog numbers■

Validation is recommended prior to use■ vailable in multiple reaction sizes and fluorophoreAchoices

For More Information
Web: bio-rad.com/ddPCR-CellAndGeneTherapy
Download bulletin: 7425

ddPCR Expert Design Assays
ddPCR Expert Design assays are designed specificallyfor Droplet Digital PCR by the experts that developed thetechnology. They encompass a wide variety of applications,
including translocations, pathogen detection, multiplexes, etc.
■■

 urated list of assays available for easy ordering on
C
Bio-Rad’s Digital Assays website (bio-rad.com/digitalassays/featured-content/expert-designs)
Validation is recommended prior to use

New assays are added regularly. Contact your Bio-Rad
representative for updates.
For More Information
Web: bio-rad.com/digitalpcr
Request or download bulletin: 6512

PREvalence ddPCR SARS-CoV-2 Wastewater Quantification & Fecal Indicator Kits
The PREvalence ddPCR SARS-CoV-2 wastewater
quantification kit provides reproducible, quantitative
results in wastewater testing for SARS-CoV-2, the virusthat causes COVID-19. The PREvalence ddPCR SARSCoV-2 wastewater quantification kit, based on thereverse transcription PCR (RT-PCR) primers used by
the Centers for Disease Control and Prevention (CDC)and World Health Organization (WHO) for SARS-CoV-2
detection, allows for the detection of N2 and E genes in
SARS-CoV-2. It also includes an assay for the murine
coronavirus, or mouse hepatitis virus (MHV), an enveloped,
single-stranded RNA virus. MHV serves as a matrix
recovery control, enabling estimation of virus lost during
sample processing. The kit allows for all three targets to
be detected in a single well. The kit contains reagents
sufficient for 200 reactions and there is also an automatedsoftware tool available for analysis.
Benefits:
Addresses an unmet need in COVID-19 surveillance —the ability to test millions of people with sufficient leadtime to take action■■

Quantitative without requiring a standard curve■

More inhibitor-resistant compared to qPCR

■

■

■

 eproducible results across runs, reagent lots,Rand instruments
 alidated on the QX200 ddPCR platform; compatibleVwith the QX ONE ddPCR platform
Aligned to CDC guidelines on wastewater testing

For More Information
Web: bio-rad.com/en-us/PREvalence

Ordering Information
Catalog #Description

12015402

PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, 200 reactions, includes ddPCR triplex
assay (SARS-CoV-2 N2 and E; MHV), supermix for one-step RT ddPCR advanced mix for probes, RT for
one-step RT ddPCR advanced mix for probes, DTT, and positive control

12015961

PREvalence ddPCR crAssphage Fecal Indicator Assay, 100 reactions, includes ddPCR crAssphage
fecal indicator assay used with the PREvalence ddPCR SARS-CoV-2 wastewater quantification kit(12015402)
PREvalence ddPCR PMMoV Fecal Indicator Assay, 100 reactions, includes ddPCR PMMoV fecal indicator
assay used with the PREvalence ddPCR SARS-CoV-2 wastewater quantification kit (12015402)

12015962

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 357

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

ddPCR Library Quantification Kit
The ddPCR library quantification kit is the optimal solution
for quality control of NGS libraries. This kit has been
experimentally validated to ensure optimal performance.
The assay generates data plots that are rich with qualitative
library information, a feature not available with othermethodologies such as quantitative PCR. With absolute
quantification, standard curves and size adjustment
calculations are not necessary to obtain precise libraryquantitation. When paired with the QX200, QX ONE or
QX600 Droplet Digital PCR System, this kit allows accuratequantification and provides a qualitative measure of the DNA
library prior to sequencing on an Illumina® platform.
■■ ontains all the necessary components to createCdroplets and quantify NGS libraries■

 nables more efficient and consistent loading andEbalancing of libraries for sequencing runs

For More Information
Web: bio-rad.com/digitalpcr
Download bulletin: 6402

 rovides both quantitative and qualitative data (includingP
adapter dimers and relative library size diversity)

Ordering Information
Catalog #Description

1863040

 dPCR Library Quantification Kit for Illumina® TruSeq®, includes 1 vial of primers and probesdat 20x concentration, 2x ddPCR supermix for probes (no dUTP)

358 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Digital PCR
bio-rad.com/digitalpcr

Droplet Digital PCR Assays and Kits

ddPCR Multiplex Mutation Screening Kits
Droplet Digital PCR (ddPCR) multiplex mutation screening
kits are designed for rapid screening of several key cancer
mutations in a single reaction with high precision andsensitivity. These kits are ideal for use with FFPE and plasma
samples, fresh/frozen tissue, and samples with low yield or
inhibitory substances. The kits contain an optimized, validated
ddPCR mutation screening multiplex assay and ddPCR
supermix for probes (no dUTP).
■

Screen multiple mutations in a single well■

Detect with high sensitivity (limit of detection ≤0.5%)

■

■

■

 rocess numerous samples in a rapid, cost-effectivePmanner ork with low amounts of input DNA without
W
preamplification
 se with Bio-Rad’s QX200, QX200 AutoDG, andU
QX ONE Droplet Digital PCR systems

Note: For research use only.
Mutations Detected by ddPCR Multiplex Mutation Screening Kits
Catalog Number ddPCR Multiplex Mutation Screening Kit*

Mutations Detected

12001037

BRAF V600

V600E, V600K, V600R

1863506

KRAS G12/G13

G12A, G12C, G12D, G12R, G12S, G12V, G13D

12001626

KRAS Q61

Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R

12001094

NRAS G12

G12A, G12C, G12D, G12R, G12S, G12V

12001627

NRAS G12/G13

G12A, G12C, G12D, G12S, G12V, G13D, G13R, G13V

12001006

NRAS Q61

Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R

12002392

EGFR exon 19 deletions>15 EGFR exon 19 deletions* Assays for each of the mutations detected by these kits can also be ordered individually. Each kit contains 20x ddPCR mutation screening
multiplex assay (1 x 200 μl) and 2x ddPCR supermix for probes (no dUTP) (2 x 1 ml).
For More Information
Web: bio-rad.com/digitalpcr
Request or download bulletin: 6782

Ordering Information
Catalog #Description

12001037
1863506
12001626
12001094
12001627
12001006
12002392

 dPCR BRAF V600 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for probes (no dUTP)
dddPCR KRAS G12/G13 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for probes (no dUTP)
ddPCR KRAS Q61 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for probes (no dUTP)
ddPCR NRAS G12 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for probes (no dUTP)
ddPCR NRAS G12/G13 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for probes (no dUTP)
ddPCR NRAS Q61 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for probes (no dUTP)
ddPCR EGFR Exon 19 Deletions Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix
for probes (no dUTP)

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 359

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

ddPCR SMN1 Copy Number Determination Kit
The ddPCR SMN1 copy number determination kit can beused for copy number determination of the survival motor
neuron 1 (SMN1) gene. It contains a duplex assay, ddPCR
supermix for probes (no dUTP) and positive controls for
0, 1, and 2 copies of SMN1.
■

Predesigned and wet-lab validated copy number assay■

Uniform cycling conditions■

 ontains all the necessary components to createCdroplets and determine copy number of the SMN1 target

For More Information
Web: bio-rad.com/digitalpcr
Download bulletin: 6630

Ordering Information
Catalog #Description

1863500 dPCR SMN1 Copy Number Determination Kit, includes assay at 20x concentration,d
2x ddPCR supermix for probes (no dUTP), and positive controls

Price, U.S. $1,875

ddPCR SMN2 Copy Number Determination Kit
The ddPCR SMN2 copy number determination kit can beused for copy number determination of the survival motor
neuron 2 (SMN2) gene. It contains a duplex assay, ddPCR
supermix for probes (no dUTP) and positive controls for
2, 3, and 4 copies of SMN2.
■

Predesigned and wet-lab validated copy number assay■

Uniform cycling conditions■

 ontains all the necessary components to createCdroplets and determine copy number of the SMN2 target

For More Information
Web: bio-rad.com/digitalpcr
Download bulletin: 6631

Ordering Information
Catalog #Description

1863503 dPCR SMN2 Copy Number Determination Kit, includes assay at 20x concentration,d
2x ddPCR supermix for probes (no dUTP), and positive controls

360 | DNA Amplification/PCR

Price, U.S. $1,875.00

In the U.S., call toll free at 1-800-424-6723

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

ddPCR Residual DNA Quantification Kits
The amount of residual host cell DNA (HCD) present
in biopharmaceutical products, such as biologics andvaccines, must meet guidelines established by regulatory
agencies, including the World Health Organization, theEuropean Pharmacopoeia, and the U.S. Food and Drug
Administration, to ensure product quality and safety. The
ddPCR CHO and E. coli residual DNA quantification (RDQ)kits allow highly sensitive and precise detection of residual
host cell DNA directly from various complex sample
matrices, eliminating the need for DNA extraction.
■■■

Highly sensitive, precise detection and quantificationof HCD without a standard curve
Direct sample input, extraction free protocol
Accurate quantification even with highly
fragmented DNA■

Fast, simple workflow■

High sample throughput

For More Information
Web: bio-rad.com/web/ddPCR/HCD
Download bulletin: 6820

Ordering Information
Catalog #Description

17000031

Vericheck ddPCR CHO Residual DNA Quantification Kit, includes 20x CHO RDQ assay and 2x ddPCR
supermix or residual DNA quantification
Vericheck ddPCR E.coli Residual DNA Quantification Kit, includes 20x E. coli RDQ assay and 2x ddPCR
supermix for residual DNA quantification

17000032

Vericheck ddPCR HEK293 Res DNA Quant Kit
Biotherapeutic products are manufactured in immortalized
cell lines, most commonly HEK293. Residual DNA from
HEK293 lines can harbor tumorigenic genetic sequences
or retroviral sequences that could be transmitted throughthe biotherapeutic to the human recipients. The Vericheck
ddPCR HEK293 residual DNA quantification kit provides anextraction-free, reproducible, and host cell–specific method
to measure residual HEK293 DNA quantity. This sensitive
method is designed and validated to provide results thatmeet the current regulatory document requirements.
■

■

■

■

 ighly specific and sensitive results without the need forHstandard curve
Reproducible, extraction-free, and easy-to-use protocol
 ositive control based autothresholding with regulatoryPcompliant software se with Bio-Rad's QX200, QX200 AutoDG, and QXUONE Droplet Digital PCR systems

For More Information
Web: bio-rad.com/ddPCR-Vericheck-HEK-Quant

Ordering Information
Catalog #Description

12016814

Vericheck ddPCR HEK293 Residual DNA Quant Kit, 100 x 20 ul reactions, includes assay, supermix, positive
control, negative control and internal control

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 361

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

Vericheck ddPCR HEK293 Res DNA Size Kit
Human gene therapy products are produced in immortalized
cell lines, most commonly HEK293. Residual DNA fromimmortalized cell lines can harbor tumorigenic genetic
sequences or retroviral sequences that can cause infection.
The regulatory guidelines suggest that manufacturers
degrade any residual DNA to a size below that of a functional
gene, defined as <200 base pairs. The Vericheck ddPCR
HEK293 sizing kit is the first HEK293 specific ddPCR based
sizing solution that has been designed, and validated tomeet regulatory guidance requirements and capable ofmeasuring HEK293 size without the use of reference curves.
■

■

■


Highlyspecific and sensitive results providing low crossreactivity and low false-positive rate eproducible, extraction-free, and easy-to-use protocol
Rwithout use of standard curves
Absolute quantification along with positive control based

autothresholding with regulatory compliant software■ se with Bio-Rad's QX200, QX200 AutoDG, and QXUONE Droplet Digital PCR systems

For More Information
Web: bio-rad.com/ddPCR-Vericheck-HEK-Size

Ordering Information
Catalog #Description

12016813

Vericheck ddPCR HEK293 Residual DNA Sizing Kit, 100 x 20 ul reactions, includes assay, supermix, positive
control, and negative control

362 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Digital PCR
Droplet Digital PCR Assays and Kits

bio-rad.com/digitalpcr

Vericheck ddPCR Mycoplasma Detection Kit
Mycoplasma is a commonly found contaminant inbiopharmaceutical development processes that are
typically not detectable by standard microscopic methods
due their small size. The European, U.S., and Japanesepharmacopoeias define the levels of Mycoplasma thatshould be present in any given product. Bio-Rad’s Vericheck
ddPCR Mycoplasma detection kit is the first Droplet Digital
PCR based Mycoplasma testing solution. The sensitive,
probe-based kit with high specificity has been designed
and validated to meet European, U.S., and Japanesepharmacopoeia requirements and can detect up to 112
Mycoplasma species. QX Manager software includes tools
to help with U.S. FDA 21 CFR Part 11 compliance along witha new positive control-based auto-thresholding feature thathas been added for turnkey, easy data analysis.
■ ultiplex probe-based assay kit designed and ValidatedM
to meet requirements from European Pharmacopoeia(EP), U.S Pharmacopoeia (USP) and JapanesePharmacopoeia (JP)■■High sensitivity and specificity
 ositive control based autothresholding with regulatoryPcompliant software

For More Information
Web: bio-rad.com/ddPCR-Vericheck-Mycoplasma

Ordering Information
Catalog #Description

12013126

Vericheck ddPCR Mycoplasma Detection Kit, 100 x 20 μl reactions, includes assay, supermix, positive control,
negative control, and internal control

Droplet Digital PCR (ddPCR) Enhancer Reagent A
Enhancer Reagent A is used in conjunction with standard
ddPCR materials in the ddPCR whole cell DNA workflow on
the QX200 manual droplet generator. The workflow enables
accurate counting of cells containing genes of interest, edits,or knockouts by encapsulating whole cells in droplets andsubsequently performing Droplet Digital PCR amplification
and analysis.

■

■

■


Easilymeasure % of single cells modified withedit(s), knockouts
 etect rare cells from low efficiency transfections orDtransductions
 aster analysis, faster on boarding compared to flowFcytometry

For More Information
Web: bio-rad.com/ddPCR-wholecell
Download bulletin: 7321

Ordering Information
Catalog #Description

12013425

ddPCR Enhancer Reagent A, 1000 reactions

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 363

Thermal Cyclers for PCR
bio-rad.com/thermalcyclers

Thermal Cyclers for PCR
Instruments range from the compact T100 thermal cycler to the PTC Tempo Thermal Cycler. Multiple modules and chassis
provide options for low- to high-throughput capabilities for the S1000.
Learn More about the Technology
Web: bio-rad.com/tech/PCR
Thermal Cycler Selection Guide
FeaturePeltier-effect technology

PTC Tempo page 365

C1000 Touch page 365

S1000 page 366

T100 page 368

•

•

•

•

Upgradable to real-time PCR•Interchangeable sample blocks••

Number of wells

96, 96 deep, 48/48, or 384

96, 96 deep, dual 48, or 384 96, 96 deep, dual 48, or 384

Gradient capability•••

Graphical user interface••

Full-color display••

Fast PCR protocol templates

•

•

•

•

Programmable ramp rates

•

•

•

•

Networking capability

•

•

•

Adjustable

Adjustable

48/48

•

•

•

•

Heated lid
Automated lid
Manual lid
USB flash drive compatibility

•

96
•
•
•

96, Deepwell, 384

Fixed
•
•
•

PTC Tempo Thermal Cyclers
Bio-Rad's PTC Tempo thermal cyclers are integrated units. The thermal cyclers are available in a 96-, Deepwell, 48/48- or
384-well plate format, accommodating different sample volume and throughput needs.
1000-Series Thermal Cyclers
Bio-Rad's 1000-series thermal cyclers offer a fully modular platform. Use the S1000 thermal cycler in standalone mode,or connect multiple S1000 cyclers to a C1000 Touch to easily run multiplate experiments. The cyclers can accommodate
different throughput needs with easily interchangeable reaction blocks.
T100 Thermal Cycler
The T100 thermal cycler is a compact, 96-well thermal cycler that offers a comprehensive package of features, including aneasy-to-use touch screen, thermal gradient, and reliable performance.

PTC Tempo Thermal Cycler

364 | DNA Amplification/PCR

C1000 Touch Thermal Cycler

S1000 Thermal Cycler

T100 Thermal Cycler

In the U.S., call toll free at 1-800-424-6723

Thermal Cyclers for PCR
bio-rad.com/PTCTempo

New PTC Tempo Thermal Cycler
The PTC Tempo thermal cycler represents the next
generation of conventional PCR thermal cyclers from
Bio-Rad. Engineered to maintain thermal performancethat delivers accurate results, the PTC Tempo thermal
cycler offers the flexibility to scale with your laboratory.
The design features a refreshed, intuitive user interface
and flexible connectivity options for streamlining protocol
management. A motorized lid enables automation, taking
your PCR to the next level with modern instrument control.
Consistent Performance
PTC Tempo thermal cyclers offer the thermal accuracy
and uniformity you can trust to get the consistent
performance you need.
■

Instant Usability
The PTC Tempo thermal cycler is factory-calibrated
and ready to use.
■

Automation Compatibility
The motorized lid built into the PTC Tempo 96,
PTC Tempo Deepwell, and PTC Tempo 384 thermal
cyclers enable integration into automated workflows.

Contemporary Connectivity
WiFi connectivity allows you to place the instrument
anywhere in the lab.
■■■

Secure User Mode
User access is protected to enhance security inregulated environments.
■■

 tore data in network drives and send email notifications
Sof completed runs.
 onnect the PTC Tempo thermal cycler to the BR.io
C
cloud platform to set up protocols remotely

For More Information
Web: bio-rad.com/PTCTempo
Request bulletins: 3322, 3328, 3329

Ordering Information
Catalog #Description

12015382
12015392

PTC Tempo 96 Thermal Cycler, includes power cord, USB cable, and Ethernet cable
PTC Tempo Deepwell Thermal Cycler, includes power cord, USB cable, and Ethernet cable

C1000 Touch Thermal Cycler
The C1000 Touch cycler is the flagship of the 1000-series
thermal cycling platforms, offering unmatched performancefor fast, reliable results. The touch-screen interface allows
ways to optimize protocols and monitor runs.
Benefits include:
■

■

■

■

■

 asily upgrade to real-time PCR using the CFX96, CFX96
E
deep well, or CFX384 optical reaction module ave time creating and viewing protocols using theSlarge color touch-screen display and intuitive graphicalprogramming
Email notification of run completion capability
Back up your data and manage and transfer files using a
USB flash drive

Optional log-in, restricted user privileges, and secure modefor controlled environments and file protection
In the U.S., call toll free at 1-800-424-6723■

Increase throughput simply and easily by connecting
up to 3 S1000 cyclers to your C1000 Touch

For More Information
Web: bio-rad.com/C1000Touch
Request or download bulletins: 6085 and 6094

DNA Amplification/PCR | 365

Thermal Cyclers for PCR
bio-rad.com/thermalcyclers

Ordering Information
Catalog #Description

1841100

 1000 Touch Thermal Cycler Chassis, includes power cord; does not include
Creaction module
C1000 Touch Thermal Cycler with Dual 48/48 Fast Reaction Module, includes C1000 Touchthermal cycler chassis, dual 48/48 fast reaction module
C1000 Touch Thermal Cycler with 96-Well Fast Reaction Module, includes C1000 Touchthermal cycler chassis, 96-well fast reaction module
C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module, includes C1000 Touchthermal cycler chassis, 96–deep well reaction module
C1000 Touch Thermal Cycler with 384-Well Reaction Module, includes C1000 Touch thermal
cycler chassis, 384-well reaction module
Touch-Screen Protector, for use with C1000 Touch thermal cycler, 2

1851148
18511961851197
1851138
1849010

S1000 Thermal Cycler
The S1000 thermal cycler can be used as a stand-alone,dependable instrument for PCR, or up to three S1000
cyclers can be connected to a C1000 Touch thermal cycler
to form a high-throughput multi-bay instrument. The S1000
cycler offers the same thermal performance as the
C1000 Touch cycler and lets you:■■

Choose a reaction module that suits your needs —dual 48/48-well fast, 96-well fast, 96–deep well,or 384-well format
 et optimal sealing using your favorite vessels andGsealers with the redesigned, fully adjustable heated lid

For More Information
Web: bio-rad.com/S1000
Download bulletin: 6082
Request or download bulletin: 6094

Ordering Information
Catalog #Description

1842000
1852148

S1000 Thermal Cycler Chassis, includes power cord; does not include reaction module
S1000 Thermal Cycler with Dual 48/48 Fast Reaction Module, includes S1000 thermal cycler
chassis, dual 48/48 fast reaction module
S1000 Thermal Cycler with 96-Well Fast Reaction Module, includes S1000 thermal cycler chassis,
96-well fast reaction module
S1000 Thermal Cycler with 96–Deep Well Reaction Module, includes S1000 thermal cycler
chassis, 96–deep well reaction module
S1000 Thermal Cycler with 384-Well Reaction Module, includes S1000 thermal cycler chassis,384-well reaction module

1852196
1852197
1852138
Accessories
1848000
1849000
1849001366 | DNA Amplification/PCR

Price, U.S. $USB Cable, for use with C1000, C1000 Touch, and S1000 thermal cyclers
 ube Frame, supports individual 0.2 ml tubes in the C1000, C1000 Touch, and S1000 dual 48- and 96-wellTreaction modules
Tube Frame, supports individual 0.2 ml tubes in the C1000 Touch and S1000 96–deep well reaction module

4,500.00
6,895.00
6,495.00
6,495.00
6,495.00

34.00
25.00
25.00

In the U.S., call toll free at 1-800-424-6723

Thermal Cyclers for PCR
bio-rad.com/thermalcyclers

Reaction Modules
Reaction Module Specifications

Reaction Module

96-Well Fast

Sample capacity

96 x 0.2 ml tubes or
1 x 96-well plate		

96–Deep Well

Dual 48/48-Well Fast

96 x 0.2 ml tubes,
2 x 48 x 0.2 ml tubes or
48 x 0.5 ml tubes, or
2 x 48-well plates
1 x 96-well plate		

384-Well1 x 384-well plate		

Maximum ramp rate

5°C/sec

2.5°C/sec

4°C/sec

2.5°C/sec

Temperature range

0–100°C

0–100°C

0 –100°C

0 –100°C

Temperature accuracy±0.2°C of programmed
target at 90°C

±0.2°C of programmed
target at 90°C

±0.2°C of programmed
target at 90°C

±0.2°C of programmed
target at 90°C

Temperature uniformity±0.4°C well-to-well within
10 sec of arrival at 90°C

±0.4°C well-to-well within
10 sec of arrival at 90°C

±0.4°C well-to-well within
10 sec of arrival at 90°C

±0.4°C well-to-well within
10 sec of arrival at 90°C

Thermal Gradient (available on all reaction modules)
Gradient range		Temperature differential range

30–100°C
1–24°C

Ordering Information
Catalog #Description

1840148

 ual 48/48 Fast Reaction Module, independent dual 48-well reaction module, fits C1000, C1000 Touch,Dand S1000 thermal cyclers, gradient enabled
96-Well Fast Reaction Module, fits C1000, C1000 Touch, and S1000 thermal cyclers, gradient enabled
96–Deep Well Reaction Module, fits C1000, C1000 Touch, and S1000 thermal cyclers, gradient enabled384-Well Reaction Module, fits C1000, C1000 Touch, and S1000 thermal cyclers, gradient enabled

1840196
1840197
1840138

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $2,995.00
2,695.00
2,795.00
2,795.00

DNA Amplification/PCR | 367

Thermal Cyclers for PCR
bio-rad.com/T100

See Also
PCR plastic
consumables:
page 401.
PCR and real-time
PCR reagents:
page 378.
Nucleic acidsample preparation:
page 9.

T100 Thermal Cycler
The T100 thermal cycler is a compact, 96-well thermal
cycler that offers a comprehensive package of features,
including an easy-to-use touch screen, thermal gradient,and reliable performance.
With the T100 thermal cycler, you can:
■

Video

■

Customer
Testimonial■■

bio-rad.com/
T100Customer
Testimonial

Save time programming with the intuitive touch screen
 et superior results faster by optimizing your PCR assays
Gin a single run using a thermal gradientSave valuable benchspace with the compact design
 eep your protocols organized using personalized folders
Kor a USB flash drive

For More Information
Web: bio-rad.com/T100
Request or download bulletin: 6065

Ordering Information
Catalog #Description

1861096
1862000
1863000

 100 Thermal Cycler, includes 96-well thermal cycler, power cord, tube support ringT
Tube Support Ring, extra tube support ring for use with individual tubes in the T100 thermal cycler, 2
Touch-Screen Protector, for use with T100 thermal cycler, 2

Price, U.S. $Recommended Consumables
1708890
cDNA Synthesis Kit, 25 x 20 μl reactions, includes 5x iScript reaction mix, iScript
reverse transcriptase, nuclease-free water
1708896
iScript Select cDNA Synthesis Kit, 25 x 20 μl reactions, includes 5x iScript reaction mix, iScript reverse
transcriptase, oligo(dT), random primer mix, gene specific primer enhancer solution, nuclease-free water
1708870
iTaq DNA Polymerase, 250 U (5 U/μl), includes 10x PCR buffer, 50 mM MgCl2 solution
iProof High-Fidelity DNA Polymerase, 100 U (2 U/μl), includes 5x HF buffer, 5x GC buffer,
1725301
50 mM MgCl2 solution, DMSOHSS9601
Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates, clear shell, clear well, 25
MLP9601
Multiplate High-Profile 96-Well Unskirted PCR Plates, clear, 25
MSB1001
Microseal 'B' Adhesive Seals, optically clear, 100
TBS1201
0.2 ml 12-Tube Strips without Caps, clear, 100
TCS1201
Domed 12-Cap Strips, for 0.2 ml PCR tubes and plates, clear, 200
TWI0201
0.2 ml PCR Tubes with Domed Caps, clear, 1,000368 | DNA Amplification/PCR

4,495.00
25.00
30.00
145.00145.00245.00125.0098.00
105.00165.00125.0071.00
58.00

In the U.S., call toll free at 1-800-424-6723

Real-Time PCR Systems
bio-rad.com/qpcr

Real-Time PCR Systems
Bio-Rad's CFX Opus and CFX Duet are powerful and flexible instruments, featuring 2-5 color multiplexing, advanced
optical technology, and precise temperature control with thermal gradients. The qPCR systems deliver sensitive, reliable
detection of both singleplex and multiplex real-time PCR reactions.
All systems support integrated data analysis for PrimePCR disease and biological pathway panels as well as automatedmultiplate gene expression analysis, absolute quantification, copy number variation, and high resolution melt genotyping
applications. See the selection guide below for comparative specifications.
Learn More about the Technology
Web: bio-rad.com/tech/qpcr

See Also
Total RNA extraction
kits: page 9.
Nucleic acid
electrophoresis
systems and gels:page 254.
PCR and real-time
PCR reagents:
page 378.
PrimePCR assays
and panels:
page 376.

Real-Time PCR System Selection Guide

PX1 PCR plate sealer:
page 400.
PCR plastic
consumables:
page 401.

FeatureCFX Opus 96

CFX Opus 384

CFX Opus Deepwell

CFX Duet

Base thermal cycler

Integrated

Integrated

Integrated

Integrated

Sample capacity

96 wells

384 wells

96 wells

96 wells

Sample volume

Up to 50 µl

Up to 30 µl

Up to 125 µl

Up to 50 µl

Light source

6 filtered LEDs

5 filtered LEDs

6 filtered LEDs

3 filtered LEDs

Optical detection

Diodes paired to LEDs
in shuttle

Diodes paired to LEDs
in shuttle

Diodes paired to LEDs
in shuttle

Diodes paired to LEDs
in shuttle

Excitation range

450–684 nm

450–650 nm

450–684 nm

450–535 nm

Detection range*

515–730 nm

515–690 nm

515–730 nm

515–580 nm

Multiplex capability

Up to 5 targets

Up to 4 targets

Up to 5 targets

Up to 2 targets

FRET capability

•

•

•

•

Maximum ramp rate

5ºC/sec

2.5ºC/sec

2.5°C/sec

5°C/sec

Gradient capability

•

•

•

•

Gradient range

30–100ºC

30–100ºC

30 –100°C

30 –100°C

Maximum gradient span

24ºC

24ºC

24°C

24°C

CFX qualification plate

•

•

•

•

Software requirement

CFX Maestro version 2.0
or higher

CFX Maestro version 2.0
or higher

CFX Maestro version 2.3
or higher

CFX Maestro version 2.3
or higher* Refer to system instruction manuals or bulletins 6105 and 7279 for information about useful detection ranges for specific dyes.

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 369

Real-Time PCR Systems
bio-rad.com/qpcr

CFX Opus Real-Time PCR Systems
The CFX Opus 96, 384, and Deepwell systems are thenext evolution in robust real-time PCR from your trusted
expert genomics partner. The CFX Opus systems
seamlessly integrate into your modern lab with improved
data connectivity. An updated physical and digital user
experience augments our proven shuttle optics andimproved thermal performance. This is our first system tointegrate with the BR.io cloud platform.
■

■

■

■

■

■

■

 eaction block with Bio-Rad's best everRthermal uniformity
 ew cloud and networked storage accessed directlyNfrom the instrumentImproved lid mechanism for better clearance andautomation
 ontinues to work with all the features of ourC
CFX Maestro control and analysis software ultiple filtered LEDs for easy multiplexing and
M
FRET testing uick system set up — easy installation and factoryQcalibrated optics
 niform optical system eliminating the need for regularUcalibration■■

 ensitive modern touch screen — responsive even whenS
double gloved udit and e-signature compatibility with CFX Maestro
A
Security Edition Software

For More Information
Web: bio-rad.com/cfxopus
For plates, tubes, and seals compatibility:
bio-rad.com/pcrplasticselector
Request or download bulletin: 7279

Ordering Information
Catalog #Description

12011319

CFX Opus 96 Real-Time PCR System, includes CFX Opus 96 system and communication cable.
Order CFX Maestro software separately
CFX Opus 384 Real-Time PCR System, includes CFX Opus 384 system and communication
cable. Order CFX Maestro software separately

12011452
12016658

Price, U.S. $InquireCFX Opus Deepwell Real-Time PCR System, includes CFX Opus Deepwell system andcommunication cable. Order CFX Maestro software separately

Recommended Consumables
1845098
CFX Qualification Plate, 96-well format, for use with CFX Opus 96, CFX Opus Deepwell, CFX Duet,
CFX96,* CFX96 Touch,* CFX96 Touch Deep Well,* or CFX Connect* systems; includes 1 predispensed platecontaining supermix, primer mix, nuclease-free water
1845099
CFX Qualification Plate, 384-well format, as referenced on p.372
HSP9601
Hard-Shell Low-Profile 96-Well Skirted PCR Plates, white shell, clear well, 50
HSP9655
Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white shell/white wells, package of 50
MSB1001
Microseal 'B' Adhesive Seals, optically clear, package of 100
1814000
PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate support block,sealing frame, power cord
1814030
Optically Clear Heat Seal, package of 100

595.00235.00165.003,710.00
115.00* CFX96, CFX96 Touch, CFX96 Touch deep well, and CFX Connect systems have been discontinued and are no longer available.

370 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Real-Time PCR Systems
bio-rad.com/qpcr

CFX Duet Real-Time PCR Detection System
The CFX Duet Real-Time PCR System makes uniform andprecise quantitative PCR (qPCR) accessible for any lab.
The system offers improved thermal performance and a
proprietary, accurate optical shuttle system for singleplex
and duplex assays.
■

■

■

■

■

 uick system set up — easy installation and factoryQcalibrated optics
 niform optical system eliminating the need forUregular calibration
No reference/normalizing dye required ime-saving experiment optimization withT
thermal gradient udit and e-signature compatibility with CFX Maestro
A
Security Edition software

For More Information
Web: bio-rad.com/cfxduet
Download bulletins: 3277, 3283, and 3281

Ordering Information
Catalog #Description

12016265

CFX Duet Real-Time PCR System, includes CFX Duet system and communication cable. Order
CFX Maestro software separately
CFX Maestro Software Version 2, compatible with Windows 7 and 10, includes USB installation
drive, video quick guides, PDF instruction manual, license for qbase+ software

12013758

Price, U.S. $Recommended Consumables
1845098
CFX Qualification Plate, 96-well format, for use with CFX Opus 96, CFX Opus Deepwell, CFX Duet,
CFX96,* CFX96 Touch,* CFX96 Touch Deep Well,* or CFX Connect* systems; includes 1 predispensed platecontaining supermix, primer mix, nuclease-free water
1845099
CFX Qualification Plate, 384-well format, as referenced on p.372
HSP9601
Hard-Shell Low-Profile 96-Well Skirted PCR Plates, white shell, clear well, 50
HSP9655
Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white shell/white wells, package of 50
MSB1001
Microseal 'B' Adhesive Seals, optically clear, package of 100
1814000
PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate support block,sealing frame, power cord
1814030
Optically Clear Heat Seal, package of 100

595.00235.00165.003,710.00
115.00* CFX96, CFX96 Touch, CFX96 Touch deep well, and CFX Connect systems have been discontinued and are no longer available.

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 371

Real-Time PCR Instrument Validation Tool
bio-rad.com/pcrvalidation

CFX Qualification Plate
The CFX qualification plate is an easy-to-use tool for
validating the performance of your CFX system. The plateis predispensed with an optimized assay, supermix, andnuclease-free water. The CFX qualification plate can beincorporated into an instrument qualification procedure
for easy tracking of your instrument’s performance.
Features include:■■■

2-fold discrimination with 99.7% confidence level redefined thermal cycling protocol and plate templatesP
for a streamlined workflow
 bility to generate a full PDF report with CFX Manager
Asoftware 3.1 or CFX Maestro software, version 2.0
or higher

For More Information
Web: bio-rad.com/cfxqualification
Download bulletin: 6323

Ordering Information
Catalog #Description

1845098

 FX Qualification Plate, 96-well format, for use with CFX Opus 96, CFX Opus 96Dx, CFX Opus 384 Dx,C
CFX Opus Deepwell, CFX Duet, CFX96,* CFX96 Touch,* CFX96 Touch Deep Well,* CFX Opus Deepwell
Dx, or CFX Connect* systems; includes 1 predispensed plate containing supermix, primer mix,
nuclease-free water
CFX Qualification Plate, 384-well format, for use with CFX Opus 96Dx, CFX Opus 384, CFX Opus
384 Dx, CFX Opus Deepwell Dx, CFX384, or CFX384 Touch systems; includes 1 predispensed platecontaining supermix, primer mix, nuclease-free water

1845099

Price, U.S. $595.00835.00* CFX96, CFX96 Touch, CFX96 Touch deep well, CFX384, CFX 384 Touch, and CFX Connect Systems have been
discontinued and are no longer available.

372 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Real-Time PCR Software
bio-rad.com/pcrsoftware

Real-Time PCR Software
Bio-Rad's real-time PCR software supports multi-instrument control and collection and analysis of real-time PCR data.
Probe and primer design software enables rapid design of robust real-time PCR assays. Contact your representative
if you need information about previous versions of software. For the latest software versions and the most up-to-date
information, please visit the listed product web pages.

CFX Maestro Software Version 2.3
Go from quantification cycles (Cq) to statistically driven
results with the latest CFX software. With just a few clicks,
you can turn your carefully crafted plate setup into
powerful studies with tools such as t-tests, clustergrams,
volcano plots, and one-way analysis of variance (ANOVA).
Then, prepare your results for publications and posters
with highly customizable data visualization tools. With
just one tool you can achieve the best quality data andpublication-ready results. With CFX Maestro software
you can:
■

■

■

■

Obtain statistics quickly and easily — t-tests and1-way ANOVA
 onvert complex studies into clear results — visualizeC
the largest multiplate studies with single-window andbox-and-whisker charts, scatter plots, clustergrams,
volcano plots, and heat maps
 pply customizable and high-resolution graphics —Aproduce publication-ready graphics in just a few clicks
Integrate data with PrimePCR assays — easily import
PrimePCR plate maps and reference gene controls

CFX Maestro Software Minimum Requirements
Compatible real-time PCR system
All CFX systems
Operating system

Windows 7, Windows 10 (for PC systems); Mac OS X El Capitan, Mac OS Sierra (for Mac systems)

Processor

2.4 GHzRAM

4 GB

Hard drive space

128 GB

Screen resolution

1,024 x 768 with true-color modeDrive

USB 2.0 Hi-Speed or above

For More Information
Web: bio-rad.com/cfx-maestro-software

Ordering Information
Catalog #Description

12013758

CFX Maestro Software Version 2.3, compatible with Windows 7 and 10, includes USB installation drive,
video quick guides, PDF instruction manual, license for qbase+ software

12004128

CFX Maestro Software for Mac, compatible with Mac OS X El Capitan and macOS Sierra, includes
USB installation drive, video quick guides, PDF instruction manual, license for qbase+ software; does notsupport instrument control

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $DNA Amplification/PCR | 373

Real-Time PCR Software
bio-rad.com/pcrsoftware

CFX Maestro Software, Security Edition
CFX Maestro software, Security Edition provides tools
for U.S. FDA 21 CFR Part 11 compliance so that you canstay focused on building powerful, statistically significant
studies. Security Edition uses a hardware protection key toencrypt files, track electronic signatures, and ensure thatfiles cannot be opened or edited by other programs. Now,
you can be certain that the integrity and validity of your data
are preserved. With CFX Maestro software, Security Edition
you can:■■■ et control where you need it — password protect
Gprotocol and data files so that only the intended users
have access enefit from fast and automatic tracking — time- andBdate-stamped audit trails make tracking changes easy
 e standard operating procedure (SOP) ready —Bassign user permissions and add electronic signatures
to improve SOP traceability

CFX Maestro Software, Security Edition Minimum Requirements
Compatible real-time PCR system
All CFX Systems
Operating system
PC: Windows 7, Windows 10. Mac: Mac OS X El Capitan, Mac OS Sierra
Processor
2.4 GHzRAM
4 GB
Hard drive space
128 GB
Screen resolution
1,024 x 768 with true-color modeDrive
USB 2.0 Hi-Speed or above

For More Information
Web: bio-rad.com/cfx-maestro-software-security-edition
bio-rad.com/cfx-maestro-software-security-edition-5

Ordering Information
Catalog #Description

12012832
12013028

CFX Maestro Software 2.3, Security Edition, includes 1 user license, USB installation drive, HASP HL key
CFX Maestro Software 2.3, Security Edition, includes 5 user licenses, 5 USB installation drives, 5 HASP HL keys

CFX Maestro Software, Chinese and Russian Editions
CFX Maestro software, Chinese and Russian Editions
work with the regional settings of the Windows 7 and 10
operating systems to provide localized, language-specificenvironments. The Chinese and Russian Editions also
provide hardware protection to CFX Maestro software: a

HASP hardware license (HL)-based key must be attached
to a USB port on the computer to use the software in
regional language mode.
For More Information
Web: bio-rad.com/cfx-maestro-software-chinese-edition;
bio-rad.com/cfx-maestro-software-russian-edition

Ordering Information
Catalog #Description

12012834
12012833

CFX Maestro Software 2.3, Chinese Edition, includes 1 user license, USB installation drive, HASP HL keys
CFX Maestro Software 2.3, Russian Edition, includes 1 user license, USB installation drive, HASP HL keys

374 | DNA Amplification/PCR

Price, U.S. $2,925.00
3,145.00

In the U.S., call toll free at 1-800-424-6723

Real-Time PCR Software
bio-rad.com/pcrsoftware

See Also

Precision Melt Analysis Software
Precision Melt Analysis software imports and analyzes
data files generated from the CFX96 and CFX384 real-time
PCR detection systems to genotype samples based on
the thermal denaturation properties of double-stranded
DNA, as described in the Precision Melt Software User
Guide. The software can be used for a variety of genotyping
applications, including scanning for new gene variants,
screening DNA samples for SNPs, identifying insertions/deletions or other unknown mutations, and determining
the percentage of methylated DNA in unknown samples.
The software enables you to:■■

Assign sample genotypes automatically, based on cluster
analysis, or manually, using multiple data view options,to tailor the software to the appropriate analysis
Generate a basic representation of the different clusters
based on curve shifting (homozygotes) and curve shapechange (heterozygotes) using the normalized melt curves
plot feature

■

■

■

■

■

Compare data between multiple file runs by combining
them into a single melt study — develop a standard
library of melt curve runs to analyze an unlimited numberof melt experiments without having to export data

Precision Melt
Supermix:
page 390.

 iew multiple displays of the data, including a simultaneousVdisplay of the original melt curves and the normalized plot
Export data to multiple formats, including spreadsheet,
image, XML, and HTML files
Analyze multiple experiments from a single plate using
the Well Groups feature rrange melt data or melt study data into aAcustomizable report

For More Information
Web: bio-rad.com/precision-melt-analysis-software
Download bulletin: 5798

Amplification Software Minimum System Requirements
Operating system
Processor
RAM
Hard drive space
Screen resolution
Drive

Windows 7, Windows 10
2.4 GHz
4 GB
128 GB
1,024 x 768 with true-color modeUSB 2.0 Hi-Speed or above

Ordering Information
Catalog #Description

1845025
18450151845020

Precision Melt Analysis Software, includes 2 user licenses, installation CD, 2 HASP HL keys, melt calibration kit
Precision Melt Analysis Software Only, includes 2 user licenses, installation CD, 2 HASP HL keys
Melt Calibration Kit, includes melt calibration DNA standard, melt primers, precision melt supermix

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $4,100.00
3,560.00
545.00DNA Amplification/PCR | 375

qPCR Gene Expression Assays and Arrays
bio-rad.com/primepcr

See Also
ddPCR
Gene Expression
Assays: page 356
iScript advanced
cDNA synthesis kit
for RT-qPCR:
page 395.
SsoAdvanced
universal SYBR®
Green supermix:
page 380.
SsoAdvanced universal
probes supermix:
page 381.

Videos
Product
Demonstration

qPCR Gene Expression Assays and Arrays
PrimePCR assays and arrays for real-time PCR are wet-lab validated or expertly designed to ensure optimal assay
performance and compliance with the minimum information for publication of quantitative real-time PCR experiments(MIQE) guidelines (Bustin et al. 2009).

PrimePCR Assays and Panels
Wet-Lab Validated or Expertly Designed for Guaranteed Performance
Every mRNA gene expression assay for human, mouse, and rat has been wet-lab validated to meet stringent performancestandards, providing confidence in results while eliminating time-consuming assay design and optimization. The assay
design algorithm that was optimized during the wet-lab validation process has been used to expertly design assays for ten
additional genomes that are guaranteed to perform.

bio-rad.com/
OrderPrimePCR

Product
Demonstration

bio-rad.com/
BuildCustom
PrimePCR

Wet-Lab Validationof Most Common
GenomesWide Range of Predesigned
Disease- and PathwaySpecific Panels

Complete Solution for
Real-Time PCR

Expert Design for All Assays
Design Parameter

Why It Matters

Maximum transcript coverageNot detecting the maximum number of transcripts underrepresents expression of target

Intron-spanning assay design
when possible

Genomic DNA amplification can lead to false-positive results, which is problematic when checking
for small changes in gene expression

Secondary structure avoided

Secondary structure can impede polymerase activity and reduce PCR efficiency

Single nucleotide polymorphisms(SNPs) avoided

SNPs destabilize binding; mismatching results in reduced PCR efficiency, delayed shift inquantification cycle (Cq), and underestimation of gene expression

Specific sequences identified(specificity)

Coamplification of off-target sequences can cause high PCR efficiency and/or
false-positive signals

Universal assay conditions tested

Assays work on any instrument with any protocol

Wet-Lab Validation for Human, Mouse, and Rat Assays
Design Parameter

Why It Matters

Specificity
		Validation with next-generation sequencing and melt curve analysis prevents coamplification of
off-target sequences that could cause high PCR efficiency and/or false-positive signals

Sensitivity/dynamic range		

Not understanding the detection limits of the assay could lead to high PCR efficiency and/or
false-positive signals

R2 value

Without a good R2 value, the Cq value will not be a reliable way to calculate the expression

Efficiency

High or low efficiency could cause misrepresentation of gene expression data

376 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

qPCR Gene Expression Assays and Arrays
bio-rad.com/primepcr

PrimePCR Genome and Product Summary				Expertly Designed, Wet-Lab Validated Assays

	Human, Mouse, and Rat

Expertly Designed Genomes
Rhesus Monkey, Yeast, Zebrafish, Cow, Pig,
Rabbit, Dog, Chicken, Rice, and Arabidopsis

SYBR® Green and probe assays••

PreAmp assays••

Pathway, collection, disease, andSABiosciences PCR arrays•

Custom plates••

Control assays••

Reference gene assays••

Reference gene panels•

Wide Range of Pathway and Collection PCR Arrays
Predesigned plates are available for signaling and disease
pathways to help identify and investigate key targets in abiological pathway of interest. Predesigned plates can be
modified to include user-selected assays.
Customizable 96- and 384-Well Plate Formats
Easy-to-use custom plate configurator allows users to lay
out assays on a plate exactly as they choose.
Fully Integrated with CFX Maestro Software
Full integration with CFX real-time PCR systems and data
analysis software streamlines data collection and analysis.

■

■

■

■

The PrimePCR qPCR product family includes:■■■

 redesigned primer assays — genome-wide assays
P
for SYBR® Green gene expression analysis available in200, 1,000, and 2,500 reaction sizes redesigned probe assays — genome-wide 5'
P
nuclease probe assays in several reporter dye options forgene expression analysis available in 500, 1,000,
and 2,500 reaction sizes ustom assays — order your primer and probeCsequences of interest, available in 200, 500, 1,000,
and 2,500 reaction sizes

■

■

■

 uman long noncoding RNA (lncRNA) geneH
expression assays — 20 predesigned array plates
and preamplification assay pools for streamlined
discovery. Individual assays are available in bothSYBR®- and probe-based formats for more than
14,000 lncRNA gene targets
 ustom PCR plates — custom-configured 96- andC
384-well PCR plates athway and collection PCR arrays — predesignedP
96- and 384-well PCR pathway and collection panels
 NA templates — synthetic DNA templates canD
be used as a positive control to trigger amplification forthe corresponding gene-specific assay
Experimental controls — control assays are available
for reverse transcription, RNA quality, genomic DNA
contamination, and PCR performance eference gene assays — commonly used referenceRgene assays are available to normalize for variation in
the amount of input mRNA among samples
PreAmp assays — assays for the unbiased, targetspecific preamplification of up to 100 gene targets ina single reaction

PrimePCR assays are also available for Droplet Digital
PCR. Please see page 354 for more information.
For More Information
Web: bio-rad.com/primepcr
Download bulletins: 6262, 6263, and 6290

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 377

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

PCR and Real-Time PCR Reagents

App and Video
Understanding
Real-Time
Supermixes

Bio-Rad’s PCR reagents and kits feature advanced enzyme technology and buffer formulations for reliable
amplification, robust reverse transcription, and high-quality real-time PCR data.
Go to bio-rad.com/ReagentsSelector for the PCR and qPCR Reagents Selector.

Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/
RTSupermixes

For More Information
Web: bio-rad.com/supermixes
Request or download bulletin: 7178

Real-time PCR supermixes are designed for superior qPCR
performance under any condition, with any detection
chemistry, and on any instrument.
Reagent Compatibility with Instruments
SYBR® Green qPCR Supermixes

One-Step RT-qPCR
Supermix and Kit

Probes qPCR Supermixes

SsoAdvanced
Universal
SYBR®
Green
Supermix

iTaq
Universal
SYBR®
Green
Supermix

SsoAdvanced
Universal
InhibitorTolerant
SYBR® Green
Supermix

Reliance
One-Step
Multiplex
Supermix

iTaq
Universal
SYBR®
Green
One-Step
Kit

iTaq
Universal
Probes
One-StepKit

CFX Opus 96, CFX Opus
96 Dx, CFX Opus 384,
CFX Opus 384 Dx,
CFX Opus Deepwell,
CFX Opus Deepwell Dx,
CFX Duet CFX96, CFX96
Touch*, CFX384, CFX384
Touch, CFX Connect*

•

•

•

•

•

•

•

•

•

iQ, iQ 5, MyiQ, MyiQ 2

•

•

•

•

MiniOpticon, DNA
Engine Opticon 1 and 2

•

•

•

•

•

•

•

•

•

•

•

•

•

•

StepOne/StepOne Plus••

7000, 7300, 7500, 7700,
7900HT, ViiA 7,
QuantStudio (all systems)

u

•

•

u

•

•

•

•

•

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•

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LightCycler 480

•

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LightCycler 96

•

•

•

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•

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LightCycler 1.0, 1.5, 2.0

s

s

s

s

s

s

s

s

s

s

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•

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•

•

•

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•

Real-Time qPCR
Instrument

iQ
Supermix
and iQ
Multiplex
Powermix

•

•

•

•

•

•

iQ
iTaq
SsoAdvanced
SYBR®
Universal
Universal
Probes
Probes
GreenSupermix
Supermix
Supermix

Bio-RadApplied Biosystems

Stratagene
Mx3000P, 3005P, 4000
Eppendorfrf
Mastercycler ep
realplex 2 or 4
QIAGEN/Corbett
Rotor-Gene 3000, 6000, Q
Roche

Illumina®
Eco
Thermo Fisher Scientific
PikoReal
Idaho Technology
LightScanner HR-1

•

•

•

•

•

•

•

•

•

•

LightScanner 32

s

s

s

s

s

s

s

s

s

s

• Recommended for use as is; u ROX reference setting must be turned “off”; s BSA must be added according to instrument specifications* CFX96, CFX96 Touch, CFX96 Touch deep well, and CFX Connect Systems have been discontinued and are no longer available.
378 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

Use this selection guide to choose the right product for your application.
Videos
Real-Time PCR Supermixes Selection Guide

Sso7d Technology

SsoAdvanced Universal
Supermixes

iTaq Universal
Supermixes

SsoFast EvaGreen®
Supermixes

iQ Supermixes

FeatureSso7d-driven superior
performance on any
instrument

Advanced formulation
for reliable and universal
performance

Fast qPCR with
EvaGreen®

Reliable and trusted
performance

Available detection chemistry

SYBR® Green and probe

SYBR® Green and probe

EvaGreen®

SYBR® Green and probe

Sso7d fusion protein
technology

Yes

No

Yes

No

Sensitive detection of
low-abundance targets

Excellent

Excellent

Excellent

Good

Efficient amplificationof difficult targets

Excellent

Good

Good

Good

Tolerance to PCR inhibitors

Excellent

Good

Good

Fair

Broad reaction conditions
and assay compatibility*

Excellent

Good

Good

Fair

Polymerase activation(cDNA/gDNA)

30 sec/2 min

30 sec/2 min

30 sec/2 min

3 min

Product

bio-rad.com/Ss70d

Advanced
Formulation

bio-rad.com/
PCRSupermixes

Customer
Testimonial

Fast cycling compatibility

Yes

Yes

Yes

Yes

Instrument compatibility

Both ROX-dependent(high and low) and ROXindependent

Both ROX-dependent(high and low) and ROXindependent

Select instruments

Select instruments

bio-rad.com/
viral-replication-pcr* Compatible with a broad range of primer concentrations, salt concentrations, annealing temperatures, and cycling protocols.
One-Step Reverse Transcription Quantitative PCR (RT-qPCR) Reagents Selection Guide
Product

Reliance One-Step Multiplex Supermix

iTaq Universal One-Step Kit

FeatureSingle-tube, 4x supermix for 5-plex
with 24-hour stability

Easy 2-tube system for SYBR® Greenand probe-based RT-qPCR

Available detection chemistry

Probes, multiplex up to 5 targets

SYBR® Green and probe duplex

Starting material

Purified RNA or DNA, lysate up to 10 μl

Purified RNA, lysate up to 4 μl

Product format

1 tube (4x RT-qPCR supermix)

2 tubes (reverse transcriptase + 2x qPCR supermix)

Detection sensitivity

High

High

Tolerance to PCR inhibitors

Great

Great

Assembled reaction stability

Up to 24 hours—

Instrument compatibility

Both ROX-dependent (high and low)
and ROX-independent

Both ROX-dependent (high and low)
and ROX-independent

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 379

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

SsoAdvanced Universal SYBR® Green Supermix
SsoAdvanced universal SYBR® Green supermix is an
exclusive high-performance real-time PCR supermix basedon Bio-Rad’s patented* Sso7d fusion protein technology
and advanced buffer formulation. This supermix is
uniquely formulated to provide high processivity, increased
PCR inhibitor tolerance, and robust performance withchallenging templates and targets in SYBR® Green–based
qPCR. It delivers superior performance for a wide range
of applications on any qPCR platform and is validated toensure optimal results with Bio-Rad’s PrimePCR gene
expression assays.

DNA polymerase

Sso7d

The dsDNA binding protein, Sso7d, stabilizes the polymerasetemplate complex, increases processivity, and provides greater
speed and reduced reaction times compared to traditional DNA
polymerases. Sso7d fusion polymerases are significantly more resistantAchieve superior qPCR results under various
13-0478 Sso7d Illustration Final
to PCR inhibitors, making the SsoAdvanced supermixes ideal choices
02.20.13
experimental conditions — for example, different
for challenging applications, such as direct qPCR, without the need forprimer concentrations, salt concentrations, annealing
sample preparation.

SsoAdvanced universal SYBR® Green supermix
allows you to:■temperatures of various assays, and/or cycling protocols(standard or fast)■■■I ncrease your qPCR sensitivity and detection
efficiency from compromised samples — thehighly processive Sso7d fusion polymerase has increased
resistance to various PCR inhibitors, enhancing sensitivity
and overall performance
Shorten time to results without compromising
qPCR data quality — the Sso7d fusion polymerase
and optimized buffer provide rapid polymerization 		
kinetics and instant polymerase activation
 se with any real-time PCR system — the universal
Ureference dye in this supermix enables ROX normalizationof qPCR data regardless of the ROX level
requirements of the qPCR system

Instrument Compatibility
The SsoAdvanced universal SYBR® Green supermix
is compatible with all commercially available andall Bio-Rad real-time qPCR systems

Applications and Uses of SsoAdvanced
Universal SYBR® Green Supermix
■

■

■

■

■

■

■

■

■

■

qPCR/real-time PCR
Gene expression analysis
Pathway analysis
Absolute quantification
Chromatin immunoprecipitation(ChIP) qPCR
Mutation detection
Pathogen detection
Viral detection (load)
Genetically modified organism(GMO) testing
Genetic profiling

For More Information
Web: bio-rad.com/supermixes
View the Understanding Real-Time PCR Supermixes Tutorial:bio-rad.com/supermixes_tutorial

Ordering Information
Catalog #Description

172527017252711725272
17252741725275

SsoAdvanced Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
SsoAdvanced Universal SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions
SsoAdvanced Universal SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions
SsoAdvanced Universal SYBR Green Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions
SsoAdvanced Universal SYBR Green Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions

Price, U.S. $160.00355.00680.001,570.00
2,985.00* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

380 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

SsoAdvanced Universal Probes Supermix
SsoAdvanced universal probes supermix is an exclusive
high-performance real-time PCR supermix based on
Bio-Rad’s patented* Sso7d fusion protein technology andadvanced buffer formulation. This supermix is uniquely
formulated to provide high processivity, increased
PCR inhibitor tolerance, and robust performance withchallenging templates and targets in probe-based qPCR.
It delivers superior performance for a wide range ofapplications on any qPCR platform and is validated
to ensure optimal results with Bio-Rad’s PrimePCR
gene expression assays.
SsoAdvanced universal probes supermix allows you to:■ arry out high-performance singleplex and duplex
C
reactions — the highly processive Sso7d fusion
polymerase and advanced formulation enable robust
performance in singleplex or duplex qPCR, allowing
cost and time savings

Applications and Uses of SsoAdvanced
Universal Probes Supermix
qPCR/real-time PCR
Gene expression analysis
Absolute quantification
Genotyping (allelic discrimination)
Mutation detection
Pathogen detection
Viral detection (load)
GMO testing
Genetic profiling
■

■

■

■

■

■

■

■

■

■

■

■

 chieve superior qPCR results under variousAexperimental conditions — for example, different
primer concentrations, salt concentrations, annealing
temperatures of various assays, and/or cycling protocols(standard or fast)Increase your qPCR sensitivity and detection
efficiency from compromised samples — thehighly processive Sso7d fusion polymerase has
increased resistance to various PCR inhibitors,
enhancing sensitivity and overall performance■

Instrument Compatibility
The SsoAdvanced universal probes supermix is compatible
with all commercially available and all Bio-Rad real-time
qPCR systems.
For More Information
Web: bio-rad.com/supermixes
View the Understanding Real-Time PCR Supermixes Tutorial:bio-rad.com/supermixes_tutorial horten time to results without compromisingS
qPCR data quality — the Sso7d fusion polymerase
and optimized buffer provide rapid polymerization
kinetics and instant polymerase activation
 se with any real-time PCR system — theUuniversal reference dye in this supermix enables ROX
normalization of qPCR data regardless of the ROX
level requirements of the qPCR system

Ordering Information
Catalog #Description

1725280
17252811725282
17252841725285

SsoAdvanced Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
SsoAdvanced Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions
SsoAdvanced Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions
SsoAdvanced Universal Probes Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions
SsoAdvanced Universal Probes Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions

Price, U.S. $155.00345.00665.001,550.00
2,960.00* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 381

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

SsoAdvanced Universal Inhibitor-Tolerant SYBR® Green Supermix
SsoAdvanced universal inhibitor-tolerant SYBR® Green
supermix is an exclusive high-performance real-time PCR
supermix based on Bio-Rad’s patented* Sso7d fusion
protein technology and advanced buffer formulation. This
supermix is specifically formulated for SYBR® Green–based
qPCR with challenging samples, including crude lysates
and inhibitor-rich samples. This supermix delivers highprocessivity, unrivaled PCR inhibitor tolerance, and robust
performance across a wide range of applications on any
qPCR platform and is validated to ensure optimal resultswith Bio-Rad’s PrimePCR gene expression assays.

■

SsoAdvanced universal inhibitor-tolerant SYBR® Green
supermix allows you to:
■

■

■

■

 kip RNA and DNA extraction by using crude
Ssamples — achieve superior data using crude lysatesfrom plants, seeds, cells, bacteria, or formalin-fixed,
paraffin-embedded (FFPE) samples
Power through PCR inhibitors — delivers unrivaled
performance with common PCR inhibitors such as
heparin, polysaccharides, culture media, and moreIncrease cDNA loading — add up to 20% cDNA from
our iScript advanced cDNA synthesis kit for RT-qPCR
or iScript reverse transcription supermix for RT-qPCR btain high-quality qPCR results under variousOexperimental conditions — for example, different
primer concentrations, annealing temperatures, salt
concentrations, and/or cycling protocols (standard
or fast)■ horten time to results without compromising qPCR
S
data quality — the Sso7d fusion polymerase andoptimized buffer provide rapid polymerization kinetics
and instant polymerase activation
 se with any real-time PCR system — the universal
Ureference dye in this supermix enables ROX normalizationof qPCR data regardless of the ROX level requirementsof the qPCR system

Applications and Uses of SsoAdvanced Universal
Inhibitor-Tolerant SYBR® Green Supermix
Viral and bacterial detection
GMO testingSingle nucleotide polymorphism (SNP) genotyping
Mutation detection
Environmental monitoring
Gene expression
Pathway analysis
■

■

■

■

■

■

■

Instrument Compatibility
The SsoAdvanced universal inhibitor-tolerant SYBR®
Green supermix is compatible with all commerciallyavailable and all Bio-Rad real-time qPCR systems.
For More Information
Web: bio-rad.com/SUIT

Ordering Information
Catalog #Description

172501617250171725018

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, 5 ml (1 x 5 ml vial), 500 x 20 μl reactions
SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, 10 ml (2 x 5 ml vials), 1,000 x 20 μl reactions* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

382 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

Video

iTaq Universal Supermixes

Advanced
Formulation

iTaq universal supermix is a 2x concentrated, ready-touse supermix formulated to deliver robust qPCR resultswith superior sensitivity, efficiency, and specificity. This
supermix is available with SYBR® Green or probe-based
chemistries.

bio-rad.com/
PCRSupermixes

Features and benefits include:
■

■

■

■

 dvanced buffer formulation developed for optimal
A
qPCR results with SYBR® Green or fluorogenic probe(simplex and duplex)
 ntibody-mediated hot-start iTaq DNA polymerase forAfast activation and superior specificity
 ptimized for consistent results using both standardO
and fast cycling protocols
 lend of reference dyes for universal real-time PCR
B
instrument compatibility

Instrument Compatibility
The iTaq universal SYBR® Green supermix and probes
supermix are compatible with all commercially available
and all Bio-Rad real-time qPCR systems.
For More Information
Web: bio-rad.com/supermixes
View the Understanding Real-Time PCR Supermixes Tutorial:bio-rad.com/supermixes_tutorial

Ordering Information
Catalog #Description

172512017251211725122172512417251251725130172513117251321725134
1725135

iTaq Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
iTaq Universal SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions
iTaq Universal SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions
iTaq Universal SYBR Green Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions
iTaq Universal SYBR Green Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions
iTaq Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
iTaq Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions
iTaq Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions
iTaq Universal Probes Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions
iTaq Universal Probes Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions

Price, U.S. $135.00285.00545.001,265.00
2,400.00
135.00285.00545.001,265.00
2,400.00

SsoFast EvaGreen® Supermixes
SsoFast EvaGreen® Supermix
Instant activation of antibody-mediated hot-start Sso7d
fusion polymerase generates qPCR results in less than
30 min■■■■ ensitive amplification and detection from a single
S
copy of a target gene
 fficient discrimination and reliable quantification
Efrom 1.33-fold serial dilutions of template
Minimal PCR inhibition of EvaGreen® and Sso7d
polymerase generates greater fluorescent signals,
resulting in increased sensitivity

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 383

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

SsoFast EvaGreen® Supermix with Low ROXInstant activation of antibody-mediated hot-start
Sso7d fusion polymerase generates qPCR results
in approximately 40 min
■

■

■

■

 reblended with ROX for optimal performance on
P
Applied Biosystems standard and fast 7500 and
ViiA 7 qPCR instruments
 ensitive amplification and detection from a single
S
copy of a target gene
Minimal PCR inhibition of EvaGreen® and Sso7d
polymerase generates greater fluorescent signals,
resulting in increased sensitivity

Instrument Compatibility
The SsoFast EvaGreen® supermixes are compatible
with select commercially available instruments and all
Bio-Rad real-time qPCR systems.

For More Information
Web: bio-rad.com/supermixes

Ordering Information
Catalog #Description

1725200
1725201
1725202
1725203
1725204
172520517252111725102
1725213

SsoFast EvaGreen Supermix, 2 ml (2 x 1 ml vials), 200 x 20 µl reactions
SsoFast EvaGreen Supermix, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions
SsoFast EvaGreen Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 µl reactions
SsoFast EvaGreen Supermix, 20 ml (1 x 20 ml bottle), 2,000 x 20 µl reactions
SsoFast EvaGreen Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 µl reactions
SsoFast EvaGreen Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 µl reactions
SsoFast EvaGreen Supermix with Low ROX, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions
SsoFast EvaGreen Supermix with Low ROX, 10 ml (10 x 1 ml vials), 1,000 x 20 µl reactions
SsoFast EvaGreen Supermix with Low ROX, 20 ml (1 x 20 ml bottle), 2,000 x 20 µl reactions

Price, U.S. $135.00285.00545.001,265.00
2,400.00
135.00545.001,265.00

iQ Supermixes
iQ SYBR® Green SupermixFormulated for efficient and sensitive
SYBR® Green–based PCR in a wide varietyof real-time PCR instruments
■

■

■

■

■

 ntibody-mediated hot-start polymerase forA
quick activation and increased specificity

■

■

■

■

 eliable detection of low-, medium-, andRhigh-abundance target genes

iQ SupermixFormulated for efficient and sensitive qPCR
using fluorogenic probes in a wide variety ofreal-time PCR instruments■

iQ Multiplex Powermix
Robust supermix optimized for sensitive and efficient
multiplex qPCR of up to 5 targets
 eliable quantification of up to 4 targets when toneRdiffers in expression up to 106 -fold relative to the others inearity over 6 orders of magnitude of input cDNA
L
and 4 orders of magnitude of input gDNA
 uitable for a wide variety of applications, includingS
gene expression analysis, SNP genotyping, SNP analysis,
GMO detection, and viral load detection

For More Information
Download bulletin: 6090

 eliable quantification of up to 2 targets over a wide
R
dynamic range of human genomic DNA and plasmid
DNA concentrations
 ntibody-mediated hot-start iTaq DNA polymeraseA
for quick activation and increased specificity

384 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

Ordering Information
Catalog #Description

1708880
1708882
17088841708885
1708886
1708887
1708860
1708862
1708864
170886517258481725849

iQ SYBR Green Supermix, 2.5 ml (2 x 1.25 ml vials), 100 x 50 μl reactions
iQ SYBR Green Supermix, 12.5 ml (10 x 1.25 ml vials), 500 x 50 μl reactions
iQ SYBR Green Supermix, 25 ml (20 x 1.25 ml vials), 1,000 x 50 μl reactions
iQ SYBR Green Supermix, 50 ml (50 ml bottle), 2,000 x 50 μl reactions
iQ SYBR Green Supermix, 25 ml (5 x 5 ml vials), 1,000 x 50 μl reactions
iQ SYBR Green Supermix, 50 ml (10 x 5 ml vials), 2,000 x 50 μl reactionsiQ Supermix, 2.5 ml (2 x 1.25 ml vials), 100 x 50 μl reactionsiQ Supermix, 12.5 ml (10 x 1.25 ml vials), 500 x 50 μl reactionsiQ Supermix, 25 ml (20 x 1.25 ml vials), 1,000 x 50 μl reactionsiQ Supermix, 50ml (40 x 1.25ml vials) 1000 x 50μl reactions
iQ Multiplex Powermix, 1.25 ml (1 x 1.25 ml vial), 50 x 50 μl reactions
iQ Multiplex Powermix, 5 ml (4 x 1.25 ml vials), 200 x 50 μl reactions

Price, U.S. $215.00940.001,815.00
3,560.00
1,685.00
3,125.00
210.00915.001,775.00
175.00625.00

Reliance One-Step Multiplex Supermix
Reliance one-step multiplex supermix is a ready-to-use,
4x reaction mix optimized for sensitive amplification of
up to five targets in a single reaction. Expertly formulatedwith an optimal enzyme blend including Reliance reverse
transcriptase, Sso7d DNA polymerase, and an advanced
reaction buffer, this robust supermix delivers reproducible
RT-qPCR performance in a multiplex fashion even withchallenging templates, difficult targets, or in the presenceof PCR inhibitors. With its exceptional room temperaturestability for assembled reactions, it can be easily integrated
into an automated workflow. Reliance one-step multiplex
supermix is an ideal solution for multiplexed RT-qPCR,
especially for pathogen/viral detection and high-throughput
screening applications.

Reliance one-step multiplex supermix allows
researchers to:■■

Key features and benefits of this robust supermix include:
■

■

■

■

■

 ensitive, reproducible multiplexing of up to 5 targets
S— gets the most out of your samples while reducing costs oom temperature stability of assembled reaction
R
up to 24 hours — enables use in an automatedworkflow to maximize throughput
 onvenient one-tube supermix — minimizes reaction
C
setup time and handling errors x concentrated formulation — increases detection
4
sensitivity by maximizing input volume
 olerant to common PCR inhibitors — for use withT
lysates or samples of varying quality■■

Amplify multiple targets with maximum efficiency
 ccelerate data throughput while decreasing costs andAreaction timeAchieve consistent and reproducible data, run after run treamline automated workflows by bypassing the needSfor plate refrigeration

Instrument Compatibility
Reliance one-step multiplex supermix is compatible withall commercially available and all Bio-Rad real-time qPCR
systems.
For More Information
Web: bio-rad.com/relianceonestep
Request or download bulletin: 7175

Ordering Information
Catalog #Description

12010176
12010220
12010221

Reliance One-Step Multiplex RT-qPCR Supermix, 1 ml (1 x 1 ml vial), 200 x 20 µl reactions
Reliance One-Step Multiplex RT-qPCR Supermix, 5 ml (5 x 1 ml vials), 1,000 x 20 µl reactions
Reliance One-Step Multiplex RT-qPCR Supermix, 10 ml (2 x 5 ml vials), 2,000 x 20 µl reactions

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $200.00740.00DNA Amplification/PCR | 385

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

See Also
PCR plastic
consumables:
page 401.

bio-rad.com/pcrreagents

iTaq Universal SYBR® Green One-Step Kit
The iTaq universal SYBR® Green one-step kit is a fast andconvenient solution for real-time PCR using the powerfulcombination of RNase H+ MMLV reverse transcriptase,specificity enhancer, and hot-start iTaq DNA polymerase
in one fast reaction. It provides improved PCR efficiency,
wider dynamic range, superior sensitivity and better
specificity, and inhibitor tolerance without affecting
performance, even with cell lysates.
■

■

■

■

Increase sensitivity, specificity, and efficiency —advanced formulation enables robust performance witha wide variety of sample types and target sequences
 se any real-time PCR system — the universal
Ureference dye in this reaction mix enables ROX
normalization of qPCR data regardless of the ROX level
requirements of the qPCR system chieve superior real-time PCR results under mostA
conditions — robust formulation delivers consistent
performance in fast cycling across a broad rangeof reaction conditions, primer concentrations, andtemperature ranges
 btain better results with PrimePCR assays —Oreal-time PCR assays are expertly designed and wet-lab
validated to ensure optimal assay performance

Applications and Uses of iTaq Universal SYBR®
Green One-Step Kit
Real-time PCR
Gene expression analysis
Absolute quantification
Pathogen/virus detection
■

■

■

■

Instrument Compatibility
The iTaq universal SYBR® Green one-step kit is compatible
with all commercially available and all
Bio-Rad real-time qPCR systems.
For More Information
Web: bio-rad.com/supermixes

Ordering Information
Catalog #Description

1725150

iTaq Universal SYBR Green One-Step Kit, 25 μl RT (1 vial), 1 ml (1 x 1 ml vial) qPCR supermix,
nuclease-free water (1 vial); 100 x 20 µl reactions
iTaq Universal SYBR Green One-Step Kit, 125 μl RT (1 vial), 5 ml (5 x 1 ml vials) qPCR supermix,
nuclease-free water (1 vial); 500 x 20 µl reactions

1725151

386 | DNA Amplification/PCR

Price, U.S. $200.00740.00In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

See Also

iTaq Universal Probes One-Step Kit

PCR plastic
consumables:
page 401.

The iTaq universal probes one-step kit is a fast andconvenient solution for real-time PCR using the powerfulcombination of RNase H+ MMLV reverse transcriptase andhot-start iTaq DNA polymerase in one fast reaction. It provides
improved PCR efficiency, wider dynamic range, superiorsensitivity and specificity, and inhibitor tolerance without
affecting performance, even with cell lysates.
■

■

■

■

■

Increase sensitivity, specificity, and efficiency —advanced formulation enables robust performance witha wide variety of sample types and target sequences
 btain superior results with multiplex reactions —Oenhanced chemistry enables up to 3 target amplifications at
the same time, resulting in higher data precision with fewer
pipetting steps and reduced sample usage arry out high-throughput real-time PCR screening andCvalidation — simplified workflow and reduced cycling
times enable screening and validation of a great numberof samples and targets in a short period of time
Use any real-time PCR system — the universal reference
dye in this reaction mix enables ROX normalization ofqPCR data regardless of the ROX level requirements of
the qPCR system chieve superior real-time PCR results under mostA
conditions — robust formulation delivers consistent
performance in fast cycling across a broad rangeof reaction conditions, primer concentrations, andtemperature ranges

Applications and Uses of iTaq Universal Probes
One-Step Kit
Real-time PCR
Gene expression analysis
Multiplexing
Absolute quantification
Pathogen/virus detection
■

■

■

■

■

Instrument Compatibility
The iTaq universal probes one-step kit is compatible
with all commercially available and all Bio-Rad
real-time qPCR systems.
For More Information
Web: bio-rad.com/supermixes

Ordering Information
Catalog #Description

1725140iTaq Universal Probes One-Step Kit, 50 μl RT (1 vial), 1 ml (1 x 1 ml vial) qPCR supermix,
nuclease-free water (1 vial); 100 x 20 µl reactionsi Taq Universal Probes One-Step Kit, 250 μl RT (1 vial), 5 ml (5 x 1 ml vials) qPCR supermix,
nuclease-free water (1 vial), 500 x 20 µl reactionsi Taq Universal Probes One-Step Kit, 2.5ml RT (1 vial), 50ml (1 x 50ml bottle) qPCR supermix,
nuclease-free water (1 bottle); 5,000 x 20 µl reactions

1725141
12013250

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $200.00740.00DNA Amplification/PCR | 387

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

SsoAdvanced PreAmp Supermix
SsoAdvanced PreAmp supermix is a 2x concentrated,
ready-to-use reaction master mix optimized for unbiased,target-specific preamplification of cDNA and gDNA.
This supermix preamplifies DNA templates using up to
400 PrimePCR PreAmp assays (for use downstream
with PrimePCR gene expression assays), custom
designed SYBR® Green or hydrolysis probe assays,
and TaqMan assays.

■

■

■

 nalyze up to 400 targets — when the number ofAgenes that can be analyzed in an experiment is limitedby the amount of sample, preamplification will extend theamount of sample available for analysis
 equires as little as 100 pg DNA — enables analysis ofR
any precious or limited sample, such as stem cells, laser
capture microdissections, and formalin-fixed paraffinembedded tissues
 ptimized for use with PrimePCR assays — use
O
PrimePCR PreAmp assays before downstream analysis
with PrimePCR gene expression assay

For More Information
Web: bio-rad.com/PreAmp
Download bulletin: 6576

3.00
2.25
1.50
0.75
BQ score

Sso7d Fusion Polymerase Technology Enables
Unbiased Preamplification
The SsoAdvanced PreAmp supermix utilizes ourhighly processive Sso7d fusion polymerase to enablepreamplification of up to 400 targets with minimal bias.
The increased processivity of the Sso7d fusion polymerase
ensures that all targets — even difficult targets — areamplified efficiently. This reduction of preamplification
bias is critical for ensuring accuracy of the results fromdownstream quantitative real-time PCR analysis.

0

–0.75
–1.50
–2.25

–3.00
0

20

40
60
qPCR assay number

80

100

Minimal bias observed during preamplification of 100 targets.
Preamplification was performed on a cDNA sample for ten cycles using
SsoAdvanced PreAmp supermix and 100 PrimePCR PreAmp assays.
The preamplified cDNA was diluted and used in qPCR analysis alongside
cDNA that was not subject to preamplification. The bias quantification(BQ) score shows the difference in the results (quantification cycle [Cq]value) for the samples with and without preamplification. BQ scores
close to zero indicate a lack of preamplification bias.

Ordering Information
Catalog #Description

1725160

SsoAdvanced PreAmp Supermix, 1.25 ml (1 x 1.25 ml vial), 50 x 50 μl reactions

388 | DNA Amplification/PCR

Price, U.S. $645.00In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

Video

SingleShot Cell Lysis Kits

Customer
Testimonial

SingleShot cell lysis kits rapidly generate cell lysates thatare optimized for reverse transcription quantitative PCR(RT-qPCR) analysis without RNA purification.
Features include:■■

Lysates prepared in 20 minutes■

Complete removal of genomic DNA■

Preservation of RNA integrity by an RNase inhibitor■

 imple, short protocol suitable for automation andShigh-throughput experiments■

No loss of rare transcripts from column purification■

Produces accurate and sensitive qPCR data■

Validated with PrimePCR assays and panels■

bio-rad.com/
GXWorkflow

 ompatibility with 10–100,000 adherent orCsuspension cells esults comparable to those produced usingR
purified RNA

SingleShot Cell Lysis Kit
The SingleShot cell lysis kit provides the reagentsnecessary for producing RT-qPCR–ready cell lysates inapproximately 20 minutes. Reagents for RT-qPCR are
not included.
SingleShot Two-Step Kits
SingleShot two-step kits include reagents to perform
RT-qPCR using a two-step workflow with separate cDNA

synthesis and qPCR steps. These kits provide RT-qPCR
data from cultured cells in just a little over 2 hours. The
SingleShot RNA control is included to help optimize
cell number input and the amount of lysate used in the
RT-qPCR.
SingleShot One-Step Kits
SingleShot one-step kits include reagents to perform
one-step RT-qPCR utilizing integrated cDNA synthesisand qPCR steps. These kits provide RT-qPCR data from
cultured cells in approximately 1.5 hours. The SingleShot
RNA control is included to help optimize cell number input
and the amount of lysate used in the RT-qPCR.
For More Information
Web: bio-rad.com/singleshot

Ordering Information
Catalog #Description

17250801725081SingleShot Cell Lysis Kit, 100 x 50 μl reactions
SingleShot Cell Lysis Kit, 500 x 50 μl reactions

One-Step Kits
1725070
SingleShot Probes One-Step Kit, 100 x 50 μl reactions
1725095
SingleShot SYBR Green One-Step Kit, 100 x 50 μl reactions
Two-Step Kits
1725090
SingleShot Probes Kit, 100 x 50 μl reactions
1725085
SingleShot SYBR Green Kit, 100 x 50 μl reactions

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 389

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/pcrreagents

Precision Melt Supermix (HRM)

■

■

■

■

■

Ideal solution for insertions or deletions >6 base pairs
De novo SNP discovery
 ccurate quantification of CpG methylation status forA
epigenetic studies
Ideal for mutation screening of small mutations or whenusing a primer walking approach for larger regions
 xceptional room temperature stability for highEthroughput high resolution melt (HRM) studies
 ptimized formulation containing EvaGreen® dyeO
delivers robust PCR and HRM performance

For More Information
Web: bio-rad.com/supermixes
Download bulletins: 5798 and 6137

A

B
0.02
Difference RFU■

 ensitive and specific discrimination of class I–IV SNPs
S
across a broad range of amplicons

Difference RFU

■

0.00
–0.02
–0.04
–0.06

0.02
0.00
–0.02
–0.04
–0.06
–0.08

–0.08
75

76
77 78 79
Temperature, °C

80

75

76
77 78 79
Temperature, °C

80

Exceptional stability enables high-throughput genotyping
analysis with precision melt supermix. Specific amplification andaccurate discrimination of a class IV SNP (84 bp amplicon) frommouse genomic DNA was performed on a CFX384 real-time PCR
detection system either 0 hr (A) or 48 hr (B) after reaction setup. Wildtype (n), heterozygote (n), and homozygous mutant (n) are shownin the difference plots normalized to wild-type samples. Total run time
including melt curve = 150 min. RFU, relative fluorescence units.

Ordering Information
Catalog #Description

17251101725112 recision Melt Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
PPrecision Melt Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions

390 | DNA Amplification/PCR

Price, U.S. $160.00680.00In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

ZDC Multiplex RT-PCR Assay*
The ZDC multiplex RT-PCR assay is a one-step reverse
transcription PCR (RT-PCR) assay for the simultaneous
detection of Zika (ZIKV), dengue (DENV 1–4), andchikungunya (CHIKV) viral RNAs by real-time PCR. The
assay includes all the controls needed for reliable results,
thus providing researchers with a robust tool to identify
ZIKV, DENV, and CHIKV nucleic acids in RNA extracted
from a wide range of sample types.
■

■

■

■

■

■

■

■

■

Enough to perform 200 tests
 -step procedure with RT and amplification performed in
1
a single tube
 -plex assay for the simultaneous detection of ZIKV,
3DENV 1–4, and CHIKV RNA
Internal control included for use as a fourth target in themultiplex assay
 et-lab validated on the CFX96 Touch real-time PCRW
detection system and Applied Biosystems 7500 real-time
PCR system ompatible with most quantitative PCR (qPCR) systems
Con the market
 ompatible with RNA extracted from multiple startingC
materials (serum, plasma, serum-free body fluids,
cerebrospinal fluids, and mosquitoes) igh-standard GMP manufacturing procedures used forH
key components of the kit
 ompatible with RNase P detection assays as additionalCor alternative internal control

RNase P Detection Assays
The RNase P assays are hydrolysis probe–based
assays for the detection of human RNase P nucleic
acid sequence. Available in two different configurations,Cy5.5 and TAMRA, the RNase P assay can be included
as an additional control in the ZDC multiplex RT-PCR
and other assays.
* For research use only, not for diagnostic purposes.
For More Information
Web: bio-rad.com/ZDCassay
Download bulletin: 6978

Ordering Information
Catalog #Description

12003818

ZDC Multiplex RT-PCR Assay, reverse transcription PCR assay for the detection of Zika, dengue, andchikungunya viral RNAs, 200 reactions
RNase P Assay (Cy5.5), pkg of 1, vial, 440 µl 12.5x concentrated primers/probe solution
RNase P Assay (TAMRA), pkg of 1, vial, 440 µl 12.5x concentrated primers/probe solution
ZDC Clear Control Solution, pkg of 1, 5 ml tube, for use with RNase P assay and ZDC multiplex RT-PCR assay
ZDC Buffer T, pkg of 5, 1.8 ml tubes, for use with ZDC multiplex RT-PCR assay

12004601
12004602
12004860
12005062

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $185.00DNA Amplification/PCR | 391

PCR and Real-Time PCR Reagents
Real-Time PCR Supermixes, Kits, and Assays

bio-rad.com/genomics

SARS-CoV-2 RT-PCR Kits
Reliance SARS-CoV-2 Testing Solutions
Reliance SARS-CoV-2 RT-PCR kits provide sensitiveand robust detetion of SARS-CoV-2 nucleic acid in
diagnostic testing laboratories. In the US, choose fromthe SARS-CoV-2 format. Outside of the US, you can also
select the SARS-CoV-2 only format. Each kit includes
reagents, an internal amplification control, and assay
controls in a convenient single package, which canbe stored at -20 degrees C for 6 months (from date ofshipment). The SARS-CoV-2 assay in both kits is designed
to target the nucleocapsid gene of SARS-CoV-2, and theperformance of these assays is not affected by the current
Variants of Concern as of August 2022.
■

■

■

■

■

Highly sensitive one-step RT-PCR workflow
 pecifically designed panel with primers/probes in aSsingle multiplex assay
 onvenient kit package with full controls included, fromC
Exact Diagnostics
Compatible with most common PCR instrument platforms

Reliance SARS-CoV-2 RT-PCR Kit (IVD)

Reliance SARS-CoV-2 RT-PCR Assay Kit (IVD)**
Reliable detection of SARS-CoV-2 nucleic acid is enabled
by targeting two regions, N1 and N2, of the nucleocapsid
gene with the Reliance SARS-CoV-2 RT-PCR assay kit(IVD). This product uses the same primers/probe set as
the CDC kit, and can be run on the CFX Opus 96 Dx,
CFX96 Dx, CFX96 Touch, and other IVD PCR Systems.
For More Information (outside US only):
Web: bio-rad.com/SC2CE-IVD

 he kit contains all the required reagents for 200
Treactions. The kit should be stored at –20 °C.

Reliance SARS-CoV-2 RT-PCR Assay Kit (EUA)*
Reliable detection of SARS-CoV-2 nucleic acid is enabled
by targeting two regions, N1 and N2, of the nucleocapside
gene with the Reliance SARS-CoV-2 RT-PCR kit (EUA).
This kit uses the same primers/probe set as the Centers
for Disease Control and Prevention (CDC) kit, and can be
run on both research use only (RUO) and in vitro diagnostic(IVD) systems, including the CFX Opus 96, CFX Opus 384,
CFX96 Dx, CFX96 Touch, and CFX384 Touch Real-Time
PCR Systems
For More Information:
Web: bio-rad.com/SARS-CoV-2-RTPCR-IVD

Ordering Information
Catalog #Description

12014115

Reliance SARS-CoV-2 RT-PCR Assay Kit (EUA), 200 reactions*

12015534

Reliance SARS-CoV-2 RT-PCR Assay Kit (IVD), 200 reactions**

Price, U.S. $160.00Related Products
12010176
Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 ul reactions, 1 ml
COV000Exact Diagnostics SARS-CoV-2 Negative Standard, 5 x 0.3 ml
COV019Exact Diagnostics SARS-CoV-2 Positive Standard, 5 x 0.3 ml
COVFLU
Exact Diagnostics SARS-CoV-2, Flu, RSV Run Control Positive, 5 x 0.3 ml
COVFLUNEG
Exact Diagnostics SARS-CoV-2 Flu, RSV Run Control Negative, 5 x 0.3 ml

680.00* The U.S. FDA has granted emergency use authorization (EUA) for this kit in the U.S.
** These products are CE marked for sale outside the U.S., with limited regional availability. Please check with your local sales office.
* The U.S. FDA has granted emergency use authorization (EUA) for this kit in the U.S.
** These products are CE marked for sale outside the U.S., with limited regional availability. Please check with your local sales office.

392 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Real-Time PCR Supermixes, Kits, and Assays

PrimePCR SARS-CoV-2 Variant Assays
PrimePCR Assays for SARS-CoV-2 Variant
Surveillance Using Reverse Transcription
Quantitative PCR (RT-qPCR)
Benefits of SARS-CoV-2 surveillance by RT-qPCR:
Rapidly profile the mutation fingerprint of positive
remnant samples
Rapid time to results: <60 minute protocol for use by
researchersFlexible assay formats: detect up to 5 mutations in asingle reaction well
High sensitivity: RT-qPCR assays are suitable for low
input RNA samples
Expanding menu: Can be updated as new variants ofconcern arise■■

21-0429 GXB Reliance SARS-CoV-2 Variant RT-PCR Kit Icon
FINAL

6/7/21
PrimePCR SARS-CoV-2
Variant Assays (RUO)

■

■

■

PrimePCR SARS-CoV-2 Variant Assays
Profile viral mutations associated with SARS-CoV-2
variants using new single and multiple mutation assays
designed for RT-qPCR. To enable researchers to perform
rapid surveillance, these assays can be used in a one-step
RT-qPCR workflow in conjunction with the Relianceonestep supermix and positive and negative assay controls
from Bio-Rad. Validation is recommended prior to use.
SARS-CoV-2 variant-specific and SARS-CoV-2 standard(wild-type) positive controls, as well as a negative control,
are available from Exact Diagnostics.
Wide Range of Single and Multiple Mutation Assaysfor Variants.
Predesigned single and multiple mutation assays todetect mutations associated with SARS-CoV-2 variants.
Target individual mutations or profile a selected group to
distinguish between multiple variants. Single and multiple
mutation assays are available in 200, 1000 and 2500
reaction sizes. These products can only be ordered throughour website. Visit bio-rad.com/SC2Variants to learn more.

PrimePCR SARS-CoV-2 Multiple Mutation
RT-qPCR Assays*
The SARS-CoV-2 multiple mutation assays offer the ability
to simultaneously detect five targets in one well. Each assay
includes primer/probe sets targeting four key mutations
found in current variants of concern. Its unique multiple
control contains a primer/probe set designed to specificallytarget a rarely mutated region of the SARS-CoV-2 genome.
PrimePCR SARS-CoV-2 Single Mutation Assays*
The SARS-CoV-2 single mutation assays contain primers
and probe to simultaneously detect and differentiate asingle mutation and one reference allele. Positive andnegative controls from Exact Diagnostics, a Bio-Rad
company, are available and recommended for assays thattarget SARS-CoV-2 spike gene variant mutations.
For More Information:
Web: bio-rad.com/SC2Variants* For research use only. Not for use in diagnostic procedures.

Ordering Information
Catalog #Description

Price, U.S. $Reagents and PCR Plastics
12010176

Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 ul reactions, 1 ml

COV000Exact Diagnostics SARS-CoV-2 Negative Standard, 5 x 0.3 ml

COV019Exact Diagnostics SARS-CoV-2 Positive Standard, 5 x 0.3 ml

COVA

SARS-CoV-2 S Gene Alpha Variant Control (B.1.1.7)

COVB

SARS-CoV-2 S Gene Beta Variant Control (B.1.351)

COVE

SARS-CoV-2 S Gene Epsilon Variant Control (B.1.427)

COVG

SARS-CoV-2 S Gene Gamma Variant Control (P.1)

12010176

Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 ul reactions, 1 ml

HSP9956 orequivalent**

Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white shell/white well, barcoded,
pkg of 50

MSB1001
Microseal 'B' PCR Plate Sealing Film, adhesive, optical, pkg of 100** Refer to the Hard Shell PCR Plates Brochure (Bulletin 5496) for other 96- and 384-well colored shell/white well PCR plates.680.00

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 393

PCR and Real-Time PCR Reagents
Reverse Transcription Reagentsbio-rad.com/pcrreagents

Reverse Transcription Reagents
Video
Understanding
ReverseTranscription

Bio-Rad’s reverse transcription reagents are formulated for efficient reverse transcription across a broad linear
dynamic range. A potent RNase A inhibitor protects RNA during setup and reverse transcription. Reagents haveflexible input RNA capacity to suit different experimental needs and are optimized for gene expression analysis
using real-time PCR.
For More Information
Web: bio-rad.com/RTreagentsbio-rad.com/rt_tutorial

bio-rad.com/
rt_tutorial

Product
FeatureApplications

Reliance SelectcDNA Synthesis
Kit

iScript Reverse
Transcription
Supermixfor RT-qPCR

iScript gDNA Clear iScript Advanced
cDNA Synthesis
cDNA Synthesis
Kit for RT-qPCR
Kit

iScript SelectcDNA
Synthesis Kit

cDNA Synthesis
Kit

Maximum input RNA Flexible
Reliable
Effective genomic
value solution
DNA removal before for high cDNA yields priming options
RT
■
■
■
■
■
■
Gene expression
Gene expression
Gene expression
Gene expression
Gene expression
Gene expression
■
■
■
■
■
■
Cloning
RNA
RNA
RNA
CloningRNA■■quantification
quantification
quantification
quantification
Sequencing
RNA■quantification
RNA quantification
Challenging
samples

Minimum reaction
setup time

Total input RNA 100 fg - 2 ug

1 µg–1 pg

1 µg –1 pg

7.5 µg–100 fg

1 µg–1 pg

Format7 tubes

1 tubes

3 tubes

2 tubes

5 tubes

Kit contents

5x Reaction Mix■
RT enzyme■Reliance SelectRandom Primers■
Oligo (dT)
■
DNase
■
DNase buffer■Nuclease FreeWater
10 minutes

 x iScript RT
5supermix■
No-RT control
supermix■

 x iScript RT
5supermix■
No-RT control
supermix■
DNase■
DNase buffer

■

■

■

26 min36 min21 min40 – 90 min

26 min

Low

High

High

High

High

High

Time toproduce cDNA
RNase H+
activity■■iScript reverse
transcriptase■
5x iScript
advanced
reaction mix

iScript reverse
transcriptase■
5x iScript
reaction mix■
3 priming options

1 µg–100 fg
2 tubes
iScript reverse
transcriptase■
5x iScript
reaction mix

New Reliance Select cDNA Synthesis Kit
The Reliance Select cDNA Synthesis Kit contains a newly
engineered reverse transcriptase designed to perform
well with challenging samples and targets. This kit has thecapability of processing samples with difficult inhibitorsand degraded RNA (e.g., FFPE samples) as well as targets
containing secondary structures and/or high GC content.
■■■

 ovel engineered reverse transcriptase with superiorN
performance — compatible with degraded samples such
as FFPE, inhibitors and targets with high GC content■

 0 minute reaction protocol — quickly and easily
1complete cDNA synthesis■

 igh tolerance to common reaction inhibitors — enablesHhigher reproducibility and consistent results, even insamples with inhibitor carry-over

For More Information
Web: bio-rad.com/RelianceSelect394 | DNA Amplification/PCR

Reliance cDNA Synthesis Kit

 igh versatility — stock a single reverse transcription kit
Hin the lab hermostability up to 55ºC allows for better performanceTwith secondary structures

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagents
Reverse Transcription Reagentsbio-rad.com/pcrreagents

Ordering Information
Catalog #Description

12012802

Reliance Select cDNA Synthesis Kit, 25 x 20 µl reactions, kit includes 400 µl Reliance Select 5X reaction
mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer,
nuclease-free water
Reliance Select cDNA Synthesis Kit, 100 x 20 µl reactions, kit includes 400 µl Reliance Select 5X reaction mix,
100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nucleasefree water
Reliance Select cDNA Synthesis Kit, 500 x 20 µl reactions, bundle of 5 boxes of 100 reaction kit; includes
2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers,
oligo(dT), DNase and buffer, nuclease-free water

12012801

17006111

Price, U.S. $185.00iScript Reverse Transcription Kits
iScript Advanced cDNA Synthesis Kit for RT-qPCR
Increased qPCR data throughput and cost effectivenessfrom a single 20 µl RT reaction
■

■

■

■

■

■

■

 uperior sensitivity and broad linear dynamic range forS
RT (7.5 µg–100 fg)
 -tube kit (5x iScript reaction mix and iScript reverse
2
transcriptase) for ease of use and reduced reaction
setup time
 ptimized blend of oligo(dT) and random primers ensures
O
complete and unbiased RNA sequence representation
 Nase H+ MMLV reverse transcriptase (preblendedRwith RNase inhibitor) delivers high sensitivity for reverse
transcription quantitative PCR (RT-qPCR) and eliminates
additional RNase H+ step otent blend of RNase A inhibitor protects RNA duringP
setup and RT

iScript cDNA Synthesis Kit
2-tube kit (5x iScript reaction mix and iScript reverse
transcriptase) for ease of use and reduced reaction
setup time■■■

Short 21 min protocol allows fast qPCR data generation

iScript Reverse Transcription Supermix for RT-qPCR
1-tube format for simple and fast setup and reduced
pipetting variability

■

■

■

■

■

■

■

■

■

 x formulation enables RNA volumes up to 16 µl,
5avoiding the need to concentrate your sample■

 iquid format at –20°C offers superior stability andLeliminates freeze/thaw cycle■

 uperior sensitivity and broad linear dynamic range
S
for RT (1 µg–1 pg)
 ptimized blend of oligo(dT) and random primers ensures
O
complete and unbiased RNA sequence representation
 Nase H+ MMLV reverse transcriptase (preblended withR
RNase inhibitor) delivers high sensitivity for RT-qPCR andeliminates additional RNase H+ step otent blend of RNase A inhibitor protects RNA duringP
setup and RT uperior sensitivity and broad linear dynamic range
S
for RT (1 µg–100 fg)
 ptimized blend of oligo(dT) and random primers ensures
O
complete and unbiased RNA sequence representation
 Nase H+ MMLV reverse transcriptase (preblended withR
RNase inhibitor) delivers high sensitivity for RT-qPCR andeliminates additional RNase H+ step otent blend of RNase A inhibitor protects RNA duringP
setup and RT
Short 26 min protocol allows fast qPCR data generation

iScript Select cDNA Synthesis Kit
5-tube kit (random primers, oligo(dT ), 5x iScript Selectreaction mix, iScript reverse transcriptase, and genespecific primer-enhancer solution)■■

Choice of priming strategy■

Reliable synthesis of long cDNA >6 kb in length■ uperior sensitivity and broad linear dynamic range
S
for RT (1 µg–1 pg)

Short 26 min protocol allows fast qPCR data generation

In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 395

Nucleic Acid Standards
Reverse Transcription Reagents

iScript gDNA Clear cDNA Synthesis Kit
iScript gDNA clear cDNA synthesis kit generates qPCRready cDNA free of gDNA for accurate gene expression
analysis in as little as 36 minutes. The kit consists of the
iScript reverse transcription supermix and a speciallyformulated DNase. Features and benefits include:■■

bio-rad.com/pcrreagents■■

 imple workflow and short 36 min protocol for fastS
generation of gDNA-free cDNA
 eady-to-use no RT control included in the kit toR
monitor gDNA contamination

For More Information
Download bulletin: 6090

 ffective gDNA clearance in 10 min with a speciallyEformulated DNase
 onvenient all-in-one RT supermix for simple and fastC
setup and reduced pipetting variability

Ordering Information
Catalog #Description

Price, U.S. $iScript Advanced cDNA Synthesis Kit for RT-qPCR
1725037
iScript Advanced cDNA Synthesis Kit for RT-qPCR, 25 x 20 μl reactions, includes 100 μl 5x iScript
advanced reaction mix, 25 μl iScript advanced reverse transcriptase, and nuclease-free water
1725038
iScript Advanced cDNA Synthesis Kit for RT-qPCR, 100 x 20 μl reactions, includes 400 μl 5x iScript
advanced reaction mix, 100 μl iScript advanced reverse transcriptase, and nuclease-free water
iScript Reverse Transcription Supermix for RT-qPCR
iScript Reverse Transcription Supermix for RT-qPCR, 25 x 20 μl reactions, includes 100 μl 5x iScript RT
1708840supermix, 200 μl 5x iScript RT supermix no-RT control (50 reactions), and nuclease-free water
iScript Reverse Transcription Supermix for RT-qPCR, 100 x 20 μl reactions, includes 400 μl 5x iScript RT
1708841supermix, 200 μl 5x iScript RT supermix no-RT control (50 reactions), and nuclease-free water
cDNA Synthesis Kit
1708890
cDNA Synthesis Kit, 25 x 20 μl reactions, includes 100 µl 5x iScript reaction mix,
25 µl iScript reverse transcriptase, and nuclease-free water
iScript DNA Synthesis Kit, 100 x 20 μl reactions, includes 400 µl 5x iScript reaction mix,
1708891
100 µl iScript reverse transcriptase, and nuclease-free water
iScript Select cDNA Synthesis Kit
1708896
iScript Select cDNA Synthesis Kit, 25 x 20 μl reactions, includes 400 µl iScript Select reaction mix,
25 µl iScript reverse transcriptase, 200 µl oligo(dT) mix, 200 µl random primer mix, 200 µl gene-specific primer
enhancer solution, and nuclease-free water
iScript Select cDNA Synthesis Kit, 100 x 20 μl reactions, includes 400 µl iScript Select reaction mix,
1708897
100 µl iScript reverse transcriptase, 200 µl oligo(dT) mix, 200 µl random primer mix, 200 µl gene-specific primer
enhancer, and nuclease-free water
iScript gDNA Clear cDNA Synthesis Kit
iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 μl reactions, includes 100 µl 5x iScript RT supermix, 200 µl
1725034
5x iScript no-RT control supermix, 12.5 µl iScript DNase, 150 µl iScript DNase buffer, 1.5 ml nuclease free water
iScript gDNA Clear cDNA Synthesis Kit, 100 x 20 μl reactions, includes 400 µl 5x iScript RT supermix, 400 µl
1725035
5x iScript no-RT control supermix, 50 µl iScript DNase, 150 µl iScript DNase buffer, 1.5 ml nuclease free water

396 | DNA Amplification/PCR

185.00580.00160.00485.00145.00425.00145.00450.00170.00505.00In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagentsbio-rad.com/pcrreagents

Reverse Transcription Reagents

iScript Explore One-Step RT and PreAmp Kit
The iScript Explore kit includes a highly sensitive reagent
to generate preamplified cDNA directly from RNA in asingle step. This kit is well suited for detecting manytargets from a limited sample or for reliable detection oftargets expressed at low levels. The reagent's patented*
Sso7d fusion protein provides unbiased target-specificpreamplification of up to 100 targets. The kit also integrates
an effective genomic DNA removal step, which helps
ensure only the transcripts of interest are detected byquantitative PCR.
■

■

■

■

 liminates protocol steps by completing reverseEtranscription and preamplification in a single step nables reliable detection of genes expressed atE
low levels
 acilitates highly sensitive detection of a large
Fnumber of targets
Incorporates an effective genomic DNA removal step,
providing confidence that results are only for thetranscript of interest

For More Information
Web: bio-rad.com/iscriptexplorekit

Ordering Information
Catalog #Description

12004856iScript Explore One-Step RT and PreAmp Kit, 50 x 50 µl reactions, includes 100 µl iScript advanced reverse
transcriptase, 1.25 ml 2x preamplification mix, 100 µl reaction booster, 50 µl DNase, 150 µl DNase buffer, andnuclease-free water
iScript Explore One-Step RT and PreAmp Kit, 250 x 50 µl reactions, includes 5 x 100 µl iScript advanced
reverse transcriptase, 5 x 1.25 ml 2x preamplification mix, 5 x 100 µl reaction booster, 5 x 50 µl DNase, 5 x 150 µl
DNase buffer, and nuclease-free water

17002826

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $185.00580.00DNA Amplification/PCR | 397

PCR and Real-Time PCR ReagentsStandard PCR Reagentsbio-rad.com/pcrreagentsStandard PCR Reagents
iProof High-Fidelity DNA Polymerase
■

■

■

■

 high-fidelity DNA polymerase with 52-fold moreA
accuracy than Taq DNA polymerase
Unique Pyrococcus-like proofreading enzyme is fused
to a dsDNA binding protein, Sso7d
 ong and fast PCR applications — fragments up to 37 kb
Lare amplified in less time (15–30 sec/kb) and with lessenzyme (0.25–1 U/reaction)
 onvenient 2x supermix formats available withC
GC or HF high-fidelity buffers

dNTP Mix■■ ormulated for consistency and higher efficiency
F
in PCR and qPCR
 obust dNTP solution withstands multiple roundsRof freeze-thawing and temperature cycling

iTaq DNA Polymerase■■Antibody-mediatedhot-start DNA polymerase
for quick 3 min activation at 95°C
 he hot-start polymerase prevents nonspecificTamplification and primer-dimers in both PCR andreal-time PCR applications

For More Information
Download bulletin: 2779

For More Information
Web: bio-rad.com/standardpcrreagents
Download bulletin: 5211

Ordering Information
Catalog #Description

Price, U.S. $iProof High-Fidelity DNA Polymerase, Master Mixes, and Buffers
1725300
iProof High-Fidelity DNA Polymerase, 2 U/µl, 20 U, includes 5x reaction buffers,
MgCl2 solution, DMSOiProof High-Fidelity DNA Polymerase, 2 U/µl, 100 U
1725301
1725302
iProof High-Fidelity DNA Polymerase, 2 U/µl, 500 U
1725330
iProof High-Fidelity PCR Kit, 2 U/µl, 50 U, includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs,l DNA, 1.3 and 10 kb primers, DNA standard
1725331
iProof High-Fidelity PCR Kit, 2 U/µl, 200 U, includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs,l DNA, 1.3 and 10 kb primers, DNA standard
1725310
iProof HF Master Mix, 2.5 ml, 100 x 50 µl reactions (for highest fidelity with most templates)
1725320
iProof GC Master Mix, 2.5 ml, 100 x 50 µl reactions (for GC-rich templates)
dNTP Mix
1708874

dNTP Mix, 200 µl premixed solution, contains 10 mM each dNTP (dATP, dCTP, dGTP, dTTP)

iTaq DNA Polymerase
1708870
iTaq DNA Polymerase, 5 U/µl, includes 250 U polymerase, 1.25 ml 10x PCR buffer
1708875
iTaq DNA Polymerase, 5 U/µl, includes 5,000 U polymerase, 25 ml 10x PCR buffer,
25 ml 50 mM MgCl2 solution
MgCl2
1708872

MgCl2 Solution, 50 mM, 1.25 ml

24.00
125.00500.0075.00
265.00195.00285.00130.00245.003,690.00

26.00* U.S. patent 7,560,260.

398 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR and Real-Time PCR Reagents
PCR and Real-Time PCR Accessories

bio-rad.com/pcrreagents

PCR and Real-Time PCR Accessories
ROX Passive Reference Dye■■■ ormulated as a 50x concentrated stock solution forFuse on Applied Biosystems 7000, 7300, 7700, and7900 qPCR instruments
 or instruments that use 580–585 nm excitation forFpassive reference, such as Stratagene Mx3000P,
Mx3005P, and Mx4000, and Applied Biosystems
7500 real-time PCR instruments, treat as a 750x
concentrated solution

Precision Blue Real-Time PCR Dye■■ oncentrated, ready-to-use dye to increase visibilityCof real-time PCR reaction mixtures
 ptimized for use with all Bio-Rad universal real-timeOsupermixes and one-step kits

 n internal reference is not required for any Bio-RadA
real-time detection system

For More Information
Download bulletin: 6090

Ordering Information
Catalog #Description

1725858
1725555

 OX Passive Reference Dye, 0.5 ml
R
Precision Blue Real-Time PCR Dye, 55 μl, 200x concentrated blue visualization dye for use
with real-time PCR systems

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
47.00
25.00

DNA Amplification/PCR | 399

PCR Plate Sealer
bio-rad.com/pcrplatesealer

PCR Plate Sealer
See Also
Heat sealing
films and foils:
page 412.
SsoAdvanced
universal SYBR®
Green supermix:
page 380.
iTaq universal SYBR®
Green supermix:
page 383.

PX1 PCR Plate Sealer
The PX1 PCR plate sealer consistently seals PCR plates
by providing uniform heat and pressure across an entire
microplate when sealing. This semiautomated heatsealer helps deliver reliable results by removing humanvariability from plate sealing and minimizing sample
evaporation.
The PX1 sealer features an easy-to-use, intuitive touchscreen interface. The thermal sealing process is simplified
by allowing sealing temperature and time to be modified
with the touch of a button.
■■■

 ast startup time — avoid delaying an experiment whileF
the sealer warms up
 xtremely intuitive — save time programmingE
the instrument
 uickly access sealing protocols — save programming
Q
time by using stored protocols■■

Compact footprint — accommodate crowded
laboratory benches
Fully validated — have confidence in your results

For More Information
Web: bio-rad.com/pcrplatesealer
Request or download bulletin: 6257

Ordering Information
Catalog #Description

PX1 PCR Plate Sealer
1814000
PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate
support block, sealing frame, power cord
Accessories
1814080
1814085

Sealing Frame, extra sealing frame for use with PX1 PCR plate sealer, 1Plate Support Block, extra plate support block for use with PX1 PCR plate sealer, 1

Heat Seals for PX1 PCR Plate Sealer
1814030
Optically Clear Heat Seal, package of 100
1814035
Permanent Clear Heat Seal, package of 100
1814040
Pierceable Foil Heat Seal, package of 100
1814045
Peelable Foil Heat Seal, package of 100

Price, U.S. $3,710.00

64.00
280.00115.00115.0087.00
87.00

For more information about heat seals that are compatible with the PX1 PCR plate sealer, see page 412.

400 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
Thin-Wall PCR Tubes

bio-rad.com/pcrplastics

PCR Plastic Consumables
Bio-Rad thin-wall PCR tubes, PCR plates, seals, and accessories are manufactured for optimal fit and cycling performancein a variety of thermal cyclers and real-time PCR instruments, including all Bio-Rad platforms. These high-quality
consumables are suitable for a wide variety of applications. Bio-Rad tubes, tube caps, and PCR plates are molded,
inspected, and packaged in a Class 100,000 or 10,000 cleanroom environment to prevent possible nucleic acid or
nuclease contamination, then process-sampled and tested to be negative for DNase, RNase, and human DNA.
For More Information
Web: bio-rad.com/pcrplastics
For plates, tubes, and seals compatibility: bio-rad.com/pcrplasticselector
Download bulletin: 6090

Thin-Wall PCR Tubes
PCR Tubes and Strips

Individual PCR Tubes, 0.2 and 0.5 ml
These high-profile PCR tubes have double-locking capsthat won’t pop open during cycling. PCR volume ranges
are 5–125 µl for 0.2 ml tubes and 10–200 µl for 0.5 ml
tubes. Tubes with flat, frosted caps for easy labeling areavailable in both 0.2 and 0.5 ml sizes (not suitable for realtime PCR). The 0.5 ml individual tubes with attached caps
are available in resealable plastic bags of 100 tubes.

TWI0201

PCR Tube and Cap Strips

TBI0502

TFI0201

Both tubes and caps are available in strips of 8 or 12
for use in 48- and 96-well sample blocks.
■

Tight sealing and convenient handling for multiple samples■

Choice of domed or flat optical cap strips

High-Profile PCR Tube Strips
Recommended reaction volumes are 5–125 µl.
Tube strips and domed cap strips are also available
packaged together in convenient bags sufficient for
96 samples. The resealable bags protect unused tubes
and caps from accidental contamination.
Low-Profile PCR Tube Strips
These tubes reduce the potential for condensation andalso allow greater light capture in fluorescence assays
such as those performed in real-time PCR. Low-profile
tubes are ideal for use in fast and low-volume PCR
reactions. Overall height, including flat optical caps and96-place rack, is 18.3 mm. Tube height is 15.5 mm.
Low-profile tubes are available in opaque white for
optical applications.

In the U.S., call toll free at 1-800-424-6723

Tube Strips

Cap Strips

TCS0803

TCS0801

DNA Amplification/PCR | 401

PCR Plastic Consumables
Thin-Wall PCR Tubes

Flat and Domed Cap Strips for PCR Tubes
and PCR Plates
These cap strips provide extremely tight sealing of all Bio-Rad
PCR tubes and plates during thermal cycling and cold
storage. Flat cap strips feature ultraclear upper surfaces,
which are ideal for fluorescence applications. Average lighttransmittance is 1.7-fold higher than with standard-clarity
domed cap strips. Flat caps are available in strips of 8 anddomed caps are available in strips of 8 or 12. Use of a
capping tool is recommended for proper sealing of caps
on tubes or plates.

bio-rad.com/pcrplastics

For More Information
Web: bio-rad.com/pcrplastics
For plates, tubes and seals compatibility:
bio-rad.com/pcrplasticselector

Ordering Information
Catalog #Description

Price, U.S. $Individual PCR Tubes with Attached Caps (0.2 ml)
TFI0201
PCR Tubes with Flat Caps (0.2 ml), clear, 1,000
TWI0201
PCR Tubes with Domed Caps (0.2 ml), clear, 1,000

58.00
58.00

Individual PCR Tubes with Attached Caps (0.5 ml)
TBI0502
PCR Tubes with Flat Caps (0.5 ml), clear, 800 (8 bags of 100)

43.00

High-Profile Tube Strips without Caps (0.2 ml)
TBS0201
8-Tube Strips without Caps, clear, 125 strips (1,000 PCR tubes)
TBS1201
12-Tube Strips without Caps, clear, 100 strips (1,200 PCR tubes)

98.00
125.00

Low-Profile 8-Tube Strips without Caps (0.2 ml)
TLS0801
Low-Profile 8-Tube Strips without Caps, clear, 120 strips (960 PCR tubes)
TLS0851
Low-Profile 8-Tube Strips without Caps, white, 120 strips (960 PCR tubes)

105.00110.00

Domed Cap Strips
TCS0801
Domed 8-Cap Strips, for PCR tubes and plates, clear, 130
TCS1201
Domed 12-Cap Strips, for PCR tubes and plates, clear, 200

38.00
71.00

Optical Flat Cap Strips
TCS0803
Optical Flat 8-Cap Strips, for PCR tubes and plates, ultraclear, 120

38.00

High-Profile Polypropylene Tube Strips with Domed Cap Strips (0.2 ml)
TBC0802
8-Tube Strips and Domed Cap Strips, clear, 20 bags of 12 x 8-tube strips and 12 x 8-cap strips(1,920 PCR tubes and caps)
12-Tube Strips and Domed Cap Strips, clear, 20 bags of 8 x 12-tube strips and 8 x 12-cap strips
TBC1202(1,920 PCR tubes and caps)

402 | DNA Amplification/PCR

215.00195.00In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
Thin-Wall PCR Tubes

bio-rad.com/pcrplastics

Capping Tools and Racks
96-Place PCR Tube Rack and Cover
These stackable storage units for tubes and unskirted
and semi-skirted PCR plates provide a stable platform
for preparing or centrifuging reactions.

96-Place PCR
Tube Racks
and Covers

PCR Tube Rack
The PCR tube rack conforms to ANSI/SBS standards andprovides a stable platform for PCR tubes and 96-well plates.

Easy Cap Tool

Easy Cap Tool
The Easy Cap tool provides the pressure necessary toachieve a tight seal, one tube at a time, when capping
individual or strip tubes. The narrow end securely fastens
domed caps to tubes or 96-well PCR plates. The wide end
firmly holds thin-wall 0.5 ml tubes to prevent accidental
crushing when opening or closing. The side slot allows easy
opening of tight-fitting caps without generating aerosols.
Strip Cap Tool
This tool quickly and easily seals 8- and 12-cap strips on
PCR plates or tubes. A grooved channel on one side isdesigned to seal domed caps, while the flat edge on the
opposite side seals flat caps. For best results, seal tube
strips while they are in a thermal cycler block or ina 96-place rack.

PCR Tube Rack

Strip Cap Tool

For More Information
Web: bio-rad.com/PCRplasticaccessories

Ordering Information
Catalog #Description

TRC9601
TRC0501
ECT1000
ECT2000

PCR Tube Racks, ANSI/SBS standard, white, 10
96-Place Racks, with covers, for PCR tubes and unskirted and semi-skirted microplates, assorted colors, 5
Easy Cap Tool, ensures tight seal for 0.2 ml PCR tubes or 96-well microplates
Strip Cap Tool, for sealing 8- and 12-cap strips on PCR plates or tubes

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
39.00
40.00
16.00
41.00

DNA Amplification/PCR | 403

PCR Plastic Consumables
PCR Plates

bio-rad.com/pcrplastics

PCR Plates
Multiplate 48-Well PCR Plates
The versatile, unskirted design and 48-well format
make these Multiplate unskirted PCR plates ideal forlaboratories using 48-well blocks on Bio-Rad instruments.
The plates are suitable for reaction volumes of 5–125 µl.
The polypropylene construction of Multiplate PCR plates
confers very low protein binding and excellent preservationof sample volume. When less than a full plate is needed,
these plates can be easily cut with scissors to the required
size. Two plate styles are available:■■

Multiplate High-Profile 48-Well Unskirted PCR Plate igh-profile (20.7 mm) wells, clear color —H
designed to fit in most thermal cyclers
 ow-profile (15.5 mm) wells, clear color or white —L
optimized for fast PCR and low-volume reactions
Multiplate Low-Profile 48-Well Unskirted PCR Plate

For More Information
Web: bio-rad.com/48wellpcrplates

Ordering Information

See Also
PX1 PCR plate
sealer: page 400.
Heat sealing
films and foils:
page 412.
PCR plate seals:
page 410.

Catalog #Description

MLP4801
MLL4801
MLL4851

Multiplate High-Profile 48-Well Unskirted PCR Plates, clear, 50 plates
Multiplate Low-Profile 48-Well Unskirted PCR Plates, clear, 50 plates
Multiplate Low-Profile 48-Well Unskirted PCR Plates, white, 50 plates

Price, U.S. $155.00125.00140.00Hard-Shell 96-Well PCR Plates
Hard-Shell PCR plates are specifically designed towithstand the stresses of heat sealing, thermal cycling,
and robotic handling. The patented* two-component
design features a skirt and deck molded from a rigid,
thermostable polymer. The thin-wall wells are moldedof virgin polypropylene selected for low DNA binding.
These plates can withstand –80°C storage and highcentrifugation forces, making them convenient for alcohol
precipitations. Uniform wells reduce well-to-well variability
in optical assays.
■

 hite-well option allows increased fluorescentW
signal strength■

Black alphanumeric labeling for easy well identification■

Color-coded skirts with clear or white wells■

Low-cost, user-readable barcode option

Hard-Shell Low-Profile 96-Well Skirted PCR PlatesReaction volumes of 5–125 µl (200 µl maximum)■■ ow-profile (16.06 mm) wells optimized for low-volumeLreactions and fast PCR

404 | DNA Amplification/PCR

■

■

■

Full skirt for robotic handling and labeling surface
 ootprint and well spacing that match ANSI/SBS
F
standard dimensions
Barcoded plates are available

Hard-Shell High-Profile 96-Well Semi-Skirted
PCR Plates
Reaction volumes of 5–125 µl (350 µl maximum)■■

 igh-profile (20.75 mm) wells that fit most thermalHcyclers, real-time PCR detection systems, andDNA sequencers■

Warp-free half-height skirt for improved robotic handling■

Barcoded plates are available

Hard-Shell Low-Profile 96-Well Semi-Skirted
PCR Plates
Reaction volumes of 5–125 µl (200 µl maximum)■■ ow-profile (15.50 mm) wells optimized for low-volumeLreactions and fast PCR* U.S. patents 6,340,589; 6,528,302; and 7,347,977.

In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
PCR Plates

bio-rad.com/pcrplastics

■

■

■

 emi-skirted design enables compatibility with Applied
S
Biosystems 7500 fast and ViiA 7 fast instruments
 ootprint and well spacing that match ANSI/SBS
F
standard dimensions
Barcoded plates are available

Hard-Shell 96-Well 480 PCR Plates
Hard-Shell 96-well 480 PCR plates are optimized towork on the Roche LightCycler 480 and 96 systems
with a 96-well block. They are designed to withstand thestresses of thermal cycling. Superior stability and flatness
is provided via a two-component design. Features include:■■

Rigid skirt ideal
for heat sealingThin-wall polypropylene V-shaped wells for optimal
thermal transfer and recovery of low-volume samples

 esigned specifically for the Roche LightCycler 480
D
and 96
 xtremely uniform wells that reduce well-to-well variabilityE
in real-time PCR■

Warp-free skirt and deck■

Black alphanumeric labeling for easy well identification■

Raised rims fortight sealing

The skirt and deck of a Hard-Shellplate prevent warping and shrinkage

 ootprint and well spacing that match ANSI/SBS
F
standard dimensions■

Barcoded along row A■

200 µl maximum volume

Hard-Shell High-Profile 96-Well
Semi-Skirted PCR Plate

For More Information
Web: bio-rad.com/96wellpcrplates
Download bulletin: 5496

Ordering Information
Description

Clear Wells

Hard-Shell Low-Profile 96-Well Skirted PCR PlatesWhite shell, 50 plates
HSP9601
Red shell, 50 plates
HSP9611
Yellow shell, 50 plates
HSP9621
Blue shell, 50 plates
HSP9631
Green shell, 50 plates
HSP9641
Black shell, 50 plates
HSP9661White shell, barcoded, 50 plates
HSP9901
White shell, bulk pack of 400 plates
HSP9601B

Price, U.S. $ White Wells
235.00235.00235.00235.00235.00235.00245.001,570.00

HSP9655——
HSP9635
HSP9645
HSP9665
HSP9955—

Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates, 25 plates
Clear shell
HSS9601
98.00
Green shell
HSS9641
99.00
Black shell—
Clear shell, barcoded
HSS9901110.00——
HSS9665—

Hard-Shell Low-Profile 96-Well Semi-Skirted PCR Plates, 25 plates
Clear shell
HSL9601110.00
Green shell
HSL9641110.00
Clear shell, barcoded
HSL9901110.00

HSL9605
HSL9645
HSL9905

Catalog #Price, U.S. $ Black Wells
255.00

255.00
255.00
255.00260.00115.00110.00110.00110.00—————
HSP9666——

255.00

—
—
—
—
—
—
—

Description

Hard-Shell 96-Well 480 PCR Plates
HSR9905
Hard-Shell 96-Well 480 PCR Plates, clear shell/white well PCR plate for use with Roche LightCycler 480
real-time PCR system, barcoded, rigid 2-component design, 25 plates
HSR9905K
Hard-Shell 96-Well 480 PCR Plates Kit, for use with Roche LightCycler 480 real-time PCR system,
includes 100 barcoded clear shell/white well PCR plates (4 packs of #HSR9905) and 100 Microseal 'C'
optical seals (#MSC1001)
HSR9901
Hard-Shell 96-Well 480 PCR Plates, clear shell/clear well PCR plate for use with Roche LightCycler 480
real-time PCR system, barcoded, rigid 2-component design, 25 plates
HSR9901K
Hard-Shell 96-Well 480 PCR Plates Kit, for use with Roche LightCycler 480 real-time PCR system,
includes 100 barcoded clear shell/clear well PCR plates (4 packs of #HSR9901) and 100 Microseal 'C'
optical seals (#MSC1001)

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Price, U.S. $110.00645.00110.00645.00DNA Amplification/PCR | 405

PCR Plastic Consumables
PCR Plates

bio-rad.com/pcrplastics

Multiplate 96-Well PCR Plates
Multiplate High-Profile 96-Well Unskirted PCR Plates
The single-component polypropylene construction ofMultiplate PCR plates confers very low protein binding
and excellent retention of sample. When less than a fullplate is needed, these plates are easily cut with scissors to
the required size. The plates are suitable for PCR volumesof 5–125 µl.
Multiplate Low-Profile 96-Well Unskirted PCR Plates
Multiplate low-profile PCR plates combine the unskirted
feature of the original Multiplate plate but are 5 mm lower
in overall height. The lower height (15.50 mm) reduces
the potential for condensation and offers advantages
for fast PCR, low-volume reactions, and light capture influorescence assays such as real-time PCR. A rigid top
surface provides firm handling while still allowing the plate
to be cut for use in other formats.

Multiplate High-Profile 96-Well Unskirted PCR Plate

Multiplate Low-Profile 96-Well Unskirted PCR Plate

For More Information
Web: bio-rad.com/96wellpcrplates

Ordering Information
Catalog #Description

Price, U.S. $Multiplate High-Profile 96-Well Unskirted PCR Plates
MLP9601
Multiplate High-Profile 96-Well Unskirted PCR Plates, clear, 25 plates
MLP9651
Multiplate High-Profile 96-Well Unskirted PCR Plates, white, 25 plates
MLP9631
Multiplate High-Profile 96-Well Unskirted PCR Plates, blue, 25 plates

105.00120.00115.00Multiplate Low-Profile 96-Well Unskirted PCR Plates
MLL9601
Multiplate Low-Profile 96-Well Unskirted PCR Plates, clear, 25 plates
MLL9651
Multiplate Low-Profile 96-Well Unskirted PCR Plates, white, 25 plates

105.00120.00iQ High-Profile 96-Well Semi-Skirted Real-Time PCR Plates
These semi-skirted, high-profile PCR plates are optimized
for iQ5, iCycler iQ, MyiQ2, and MyiQ real-time PCR
detection systems. The semi-skirted design adds stiffness
and a labeling surface. Plates are perforated every threecolumns for easy setup of triplicate reactions.
For More Information
Web: bio-rad.com/96wellpcrplates

Ordering Information
Catalog #Description

2239441iQ High-Profile 96-Well Semi-Skirted PCR Plates, 25 plates

406 | DNA Amplification/PCR

Price, U.S. $215.00In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
PCR Plates

bio-rad.com/pcrplastics

Microseal 96-Well PCR Plates
Microseal High-Profile 96-Well Semi-Skirted
PCR Plates
These PCR plates are designed for Applied Biosystems
0.2 ml tube cyclers, standard real-time PCR systems, andDNA sequencers. They are not recommended for use in
Bio-Rad thermal cyclers because the raised ridges aroundthe plate prevent proper sealing in these instruments.
Microseal Low-Profile 96-Well Skirted PCR Plates
These PCR plates feature single-component construction
and a skirted design that is suitable for high-throughput plate
handling. The robot-friendly design features low-binding
polypropylene construction, locator holes, and flat verticalsidewalls for secure handling and easy barcoding. Raisedrims around wells provide an excellent surface for tight sealing
with a variety of sealing methods and allow easy release of thesealer from the plate. Barcoded plates are also available.

Microseal High-Profile 96-Well Semi-Skirted PCR Plate

Microseal Low-Profile 96-Well Skirted PCR Plate

For More Information
Web: bio-rad.com/96wellpcrplates

Ordering Information
Catalog #Description

Price, U.S. $Microseal High-Profile 96-Well Semi-Skirted PCR Plates
MSS9601
Microseal High-Profile 96-Well Semi-Skirted PCR Plates, clear, 25 plates

105.00Microseal Low-Profile 96-Well Skirted PCR Plates
MSP9601
Microseal Low-Profile 96-Well Skirted PCR Plates, clear, 50 plates
MSP9605
Microseal Low-Profile 96-Well Skirted PCR Plates, barcoded, clear, 50 plates

235.00245.00In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 407

PCR Plastic Consumables
PCR Plates

bio-rad.com/pcrplastics

Hard-Shell 384-Well Standard PCR Plates
Hard-Shell PCR plates are designed to withstand the stressesof thermal cycling and robotic handling. The patented* twocomponent design provides superior stability and flatness,allowing precise positioning for automation. Features include:■■■

Reaction volumes of 1–30 µl (50 µl maximum)
 inimized well-to-well variability that results in extremelyM
uniform results in real-time PCR ompatibility with most 384-well thermal cyclers, realC
time PCR detection systems, and DNA sequencers■

White-well option for increased fluorescent signal strength■Color-coded skirts with clear or white wells■

Warp-free skirt and deck for improved robotic handling■■ ootprint and well spacing that match ANSI/SBS
F
standard dimensions
Low-cost, user-readable barcode option* U.S. patents 6,340,589; 6,528,302; and 7,347,977.
■

For More Information
Web: bio-rad.com/384wellpcrplates
Download bulletin: 5496

Ordering Information
Description

Clear Wells

Hard-Shell 384-Well Standard PCR Plates
Clear shell, 50 plates
Red shell, 50 plates
Yellow shell, 50 platesBlue shell, 50 plates
Green shell, 50 platesBlack shell, 50 plates
Clear shell, barcoded, 50 plates
Clear shell, bulk pack of 500 plates

HSP3801
HSP3811
HSP3821
HSP3831
HSP3841—
HSP3901
HSP3801B

408 | DNA Amplification/PCR

Price, U.S. $ White Wells
280.00280.00280.00280.00280.00300.002,740.00

HSP3805————
HSP3865
HSP3905—

Price, U.S. $ Black Wells
340.00335.00360.00—————
HSP3866——

Price, U.S. $335.00In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
PCR Plates

bio-rad.com/pcrplastics

Hard-Shell 384-Well 480 PCR Plates
Hard-Shell 384-well 480 PCR plates are optimized to workon the Roche LightCycler 480 and a range of Bio-Rad
and Applied Biosystems instruments. They are designed
to withstand the stresses of thermal cycling and robotic
handling. The two-component design provides superiorstability and flatness. Features include:■■

Reaction volumes of 1–30 µl (50 µl maximum)
 inimized well-to-well variability that results in extremelyM
uniform results in real-time PCR■

Warp-free skirt and deck for improved robotic handling■Black alphanumeric labeling for easy well identification■■

 ootprint and well spacing that match ANSI/SBS
F
standard dimensions
 arcoded at row A and column 24 side; available in clear
Bor white well

For More Information
Web: bio-rad.com/384wellpcrplates
Download bulletin: 5496

Ordering Information
Catalog #Description

HSR4805

 ard-Shell 384-Well 480 PCR Plates, clear shell/white well PCR plate for Roche LightCycler 480 real-time
H
PCR system, barcoded, rigid 2-component design, 50 platesHard-Shell 384-Well 480 PCR Plates Kit, for use with Roche LightCycler 480 real-time PCR system,
includes 100 barcoded clear shell/white well PCR plates (2 packs of #HSR4805) and 100 Microseal 'C'
optical seals (#MSC1001)
Hard-Shell 384-Well 480 PCR Plates, clear shell/clear well PCR plate for Roche LightCycler 480 real-time
PCR system, barcoded, rigid 2-component design, 50 platesHard-Shell 384-Well 480 PCR Plates Kit, for use with Roche LightCycler 480 real-time PCR system,
includes 100 barcoded clear shell/clear well PCR plates (2 packs of #HSR4801) and 100 Microseal 'C'
optical seals (#MSC1001)

HSR4805K

HSR4801
HSR4801K

Price, U.S. $295.00705.00295.00705.00Microseal 384-Well Skirted PCR Plates
Microseal 384-well skirted PCR plates feature singlecomponent construction and are ideal for high-throughput
thermal cycling applications.
■■

Barcoded plates are available
 ootprint and well spacing that match ANSI/SBS
F
standard dimensions

For More Information
Web: bio-rad.com/384wellplates

Ordering Information
Description

50 Plates

Price, U.S. $Barcoded, 50 Plates

Microseal 384-Well Skirted PCR Plates
Clear
White
Black

MSP3842
MSP3852
MSP3862275.00290.00290.00

MSP3846——

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $290.00DNA Amplification/PCR | 409

PCR Plastic Consumables
PCR Seals

bio-rad.com/pcrseals

PCR Seals
Effective sealing is essential for PCR and qPCR reactions. Besides cap strips (pages 401–402), Bio-Rad offers many
sealing options to fit your needs.

PCR Plate Seals
Microseal 'C' Optical Seals
Optically clear adhesive films designed fortight seals even on wells with raised rims■■■

 ressure-sensitive adhesive allows easy applicationP
during plate sealing

Microseal 'C' Optical Seals esigned with superior optical properties forDreal-time PCR

Microseal 'B' Adhesive Seals, Optically Clear
Strongest adhesive-based optically clear sealing
option designed for real-time PCR plates■■ ithstands multiple storage or transport temperatures
W(–40 to 110°C)

Microseal 'A' FilmA nonoptical, nonadhesive sealing option for quickpressure-based sealing of plates

Microseal 'B'
Adhesive Seals

■

■

■

 llows easy removal without the risk of aerosol formation,A
minimizing cross-contamination
Convenient option for standard PCR needs

Microseal 'F' Foil
Aluminum foil allows opaque sealing option for DNA
sequencing (ABI 3700 DNA analyzer) and sample storage■■■

Microseal 'A' Film cts as a barrier against evaporation in extremeA
temperatures (–80 to 105°C)
Pierceable foil for easy sample retrieval

Microseal 'F' Foil

410 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
PCR Seals

bio-rad.com/pcrseals

Pressure Pad
This foam pad with magnet distributes lid pressure
uniformly over sealing film on plates used inthermal cyclers.
Optical Compression Pad (96-well)This compression pad enhances the seal integrity ofMicroseal 'B' clear seals when used in real-time PCR
detection systems.

Optical Compression Pad ptical Film Sealing Kit
O
The sealing kits contain 100 Microseal 'B' clear seals
and an optical compression pad.
For More Information
Web: bio-rad.com/pcrseals

Sealing Roller

Ordering Information
Catalog #Description

MSC1001
MSA5001
MSB1001
MSF1001
MSR0001
ADR3296

Microseal 'C' Optical Seals, 100 pressure-sensitive adhesive seals
Microseal 'A' Film, 50 seals
Microseal 'B' Adhesive Seals, 100 optically clear seals
Microseal 'F' Foil, 100 adhesive seals
Sealing Roller, for sealing PCR plates with films
Optical Compression Pad, for improved film sealing of 96-well plates in DNA Engine Opticon 2
and Chromo4 systems
Pressure Pad, uniformly distributes lid pressure for sealing film
Optical Film Sealing Kit, for 96-well plates, includes optical compression pad,
100 Microseal 'B' clear adhesive seals

ADR5001
MSO1001

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $215.0090.00
165.00105.00
22.00
22.00
16.00
170.00DNA Amplification/PCR | 411

PCR Plastic Consumables
PCR Seals

See Also
PX1 PCR plate
sealer: page 400.
PCR plates:
page 404.

bio-rad.com/pcrseals

Heat Sealing Films and Foils
Bio-Rad offers a family of heat sealing films and foils foruse with the PX1 PCR plate sealer. These sealing solutions
help deliver consistent and reliable data by minimizing
sample evaporation during thermal cycling.
For More Information
Web: bio-rad.com/heatseals
Request or download bulletin: 6257

Optically Clear Heat Seals
These clear polymer films provide a secure sealing option
for PCR and real-time PCR applications. The seals arepeelable pre- and post-cycling, allowing easy sample
retrieval when desired. Features include:■■

High light transmission, ideal for optical assays
 ow level of autofluorescence to minimize interferenceLwith qPCR detection■

Clear film enables easy inspection of sample wells■

Seal integrity from –80 to 110°C

Permanent Clear Heat Seal
These seals provide the strongest heat sealing option andare ideal when seals will not be removed. These seals arerecommended for water bath cycling. Features include:■

Permanent Clear Heat Seal trong permanent adhesive can withstand waterSbath cycling■

Clear film enables easy inspection of sample wells■

High solvent resistance■Seal integrity from –80 to 110°C

Pierceable Foil Heat Seal
This seal provides a secure sealing option for standard
PCR and Droplet Digital PCR (ddPCR) applications. Thispierceable foil film enables sample retrieval from select wells.
Plates with pierced seals can be resealed with anotherpierceable foil heat seal. Features include:■

Optically Clear Heat Seal ompatible with Droplet Digital PCR as validated usingC
the QX200 ddPCR system workflow■

High solvent resistance■

Colored stripe clearly identifies sealing surface■

Seal integrity from –20 to 110°C

Pierceable Foil Heat Seal

Peelable Foil Heat Seal
The peelable foil heat seal is ideal for sample storage. This seal
can be easily peeled from PCR plates stored in a –80°C
freezer or in liquid nitrogen. This seal is also validated forsealing PCR plates. Features include:■Forms a peel-away seal■

Moderate solvent resistance■

Seal integrity from –200 to 110°C

412 | DNA Amplification/PCR

Peelable Foil Heat Seal

For More Information
Web: bio-rad.com/heatseals
Request or download bulletin: 6257

In the U.S., call toll free at 1-800-424-6723

PCR Plastic Consumables
PCR Seals

bio-rad.com/pcrseals

Ordering Information
Catalog #Description

1814030
1814035
1814040
1814045

Optically Clear Heat Seal, package of 100
Permanent Clear Heat Seal, package of 100
Pierceable Foil Heat Seal, package of 100
Peelable Foil Heat Seal, package of 100

Price, U.S. $115.00115.0087.00
87.00

Sealing Pads for Automation
Microseal 'P' and 'P+' Sealing Pads
These reusable sealing pads are designed to adhere to a
motorized heated lid. Use 'P' pads with Power Bonnet lids,
and 'P+' pads with Moto Alpha units. Microseal 'P+' pads
provide improved sealing of low-volume reactions — as
low as 5 µl in 96-well plates and 1 µl in 384-well plates.
Each pad may be used for approximately 25 runs.

Auto-Sealing Lids for PCR Plates
These lids are reusable, automation-friendly sealers thatprevent evaporation and contamination during reaction
assembly and seal tightly for thermal cycling when thecycler lid is closed. They can be reused up to 50 times.
The lids are constructed of metal with an attachedcompressible pad. Two varieties are available: a selfreleasing arched lid and an arched lid with wide tabs
for robotic grippers.
For More Information
Web: bio-rad.com/pcrseals

Microseal 'P+' Sealing Pad

Auto-Sealing Lids

Ordering Information
Catalog #Description

Price, U.S. $Microseal 'P' and 'P+' Pads
MSP1001
Microseal 'P' Pads, reusable, for Power Bonnet lids, 10
MSP1002
Microseal 'P+' Pads, reusable, for Moto Alpha unit lids, 10
MSP1003
Microseal 'P' Replacement Pads, for MSL2032, reusable, 10

97.00
97.00
97.00

Auto-Sealing Lids for PCR Plates
MSL2022
Arched Auto-Sealing PCR Plate Lids, reusable, 4MSL2032
Arched Auto-Sealing PCR Plate Lids with Wide Tabs, reusable, 4135.00165.00In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR | 413

PCR Plastic Consumables
PCR Seals

bio-rad.com/pcrseals

Frame-Seal Incubation Chambers
Frame-Seal incubation chambers are easy-to-use, strongly
adhesive hybridization chambers with flexible plastic
coverslips. They provide vapor-tight sealing for FISH,
polonies, in situ PCR, and PRINS and allow samples tobe recovered easily. The seal withstands temperatures up
to 97°C. Frame-Seal chambers should be used with plain(unprinted) glass slides. Slides with highly hydrophobic
ink patterns are not recommended for use with FrameSeal chambers. They are UV-treatable for inactivation ofcontaminating DNA.

SLF0601
SLF1201

SLF0201

For More Information
Web: bio-rad.com/pcrseals

Ordering Information
Catalog #Description

SLF0201
SLF0601
SLF1201

Frame-Seal Incubation Chambers, 9 x 9 mm, 25 µl capacity, coverslips included, 100
Frame-Seal Incubation Chambers, 15 x 15 mm, 65 µl capacity, coverslips included, 100
Frame-Seal Incubation Chambers, 17 x 28 mm, 125 µl capacity, coverslips included, 100

414 | DNA Amplification/PCR

Price, U.S. $
88.00
94.00
110.00In the U.S., call toll free at 1-800-424-6723

DNA Amplification/PCR
Subject Index

Subject Index
C1000 Touch Thermal Cycler, 365

One-Step RT-ddPCR Advanced Kit for Probes, 351

Capping Tools and Racks, 403

PCR Plate Seals, 410

CFX Maestro Software, 373

PCR Tubes and Strips, 401

CFX Maestro Software, Chinese and Russian Editions, 374

Precision Melt Analysis Software, 375

CFX Maestro Software, Security Edition, 374

Prevalence ddPCR SARS-CoV-2 Wastewater Quantification &Fecal Indicator Kits, 357

CFX Opus Real-Time PCR Systems, 370
CFX Duet Real-Time PCR System, 371
CFX Qualification Plate, 372
ddPCR 96-Well Plates, 352
ddPCR Enhancer Reagent A, 363
ddPCR Library Quantification Kit, 358
ddPCR Multiplex Mutation Screening Kits, 359
ddPCR Multiplex Supermix, 350
ddPCR Residual DNA Quantification Kits, 361
ddPCR SMN1 Copy Number Determination Kit, 360
ddPCR SMN2 Copy Number Determination Kit, 360
ddPCR Supermix for Probes, 350
ddPCR Supermix for Probes (No dUTP), 349
ddPCR Supermix for Residual DNA Quantification, 351
Droplet Digital PCR Assays, 356
Frame-Seal Incubation Chambers, 414
Hard-Shell 96-Well PCR Plates, 404
Hard-Shell 384-Well 480 PCR Plates, 409
Hard-Shell 384-Well Standard PCR Plates, 408
Heat Sealing Films and Foils, 412
iQ High-Profile 96-Well Semi-Skirted Real-Time PCR Plates, 406
iQ Supermixes, 384
iScript Explore One-Step RT and PreAmp Kit, 397
iScript Reverse Transcription Kits, 395
iTaq Universal Probes One-Step Kit, 387
iTaq Universal Supermixes, 383
iTaq Universal SYBR® Green One-Step Kit, 386
Microseal 96-Well PCR Plates, 407
Microseal 384-Well Skirted PCR Plates, 409
Multiplate 48-Well PCR Plates, 404
Multiplate 96-Well PCR Plates, 406

In the U.S., call toll free at 1-800-424-6723

Precision Melt Supermix (HRM), 390
PrimePCR Assays and Panels, 376
PTC Tempo Thermal Cycler, 365
PrimePCR SARS-CoV-2 Variant Assays, 393
PX1 PCR Plate Sealer, 400
QX200 ddPCR EvaGreen® Supermix, 352
QX200 Droplet Digital PCR Systems, 345
QX Designer Software, 348
QX Manager Software, 347
QX600 Droplet Digital PCR Systems, 342
QX ONE Droplet Digital PCR System, 344
Reaction Modules, 367
Reliance One-Step Multiplex Supermix, 385
Reliance Select cDNA Synthesis Kit, 394
S1000 Thermal Cycler, 366
SARS-CoV-2 ddPCR Kit, 353
SARS-CoV-2 RT-PCR Kits, 392
Sealing Pads for Automation, 413
SingleShot Cell Lysis Kits, 389
SsoAdvanced PreAmp Supermix, 388
SsoAdvanced Universal Inhibitor-Tolerant SYBR®
Green Supermix, 382
SsoAdvanced Universal Probes Supermix, 381
SsoAdvanced Universal SYBR® Green Supermix, 380
SsoFast EvaGreen® Supermixes, 383
T100 Thermal Cycler, 368
Vericheck ddPCR HEK293 Res DNA Quant Kit, 361
Vericheck ddPCR HEK293 Res DNA Size Kit, 362
Vericheck ddPCR Mycoplasma Detection Kit, 363
ZDC Multiplex RT-PCR Assay, 391

DNA Amplification/PCR | 415

416 | DNA Amplification/PCR

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables
and General Laboratory
Equipment
Plastic Consumables
Pipets
Pipet Tips
Micro Test Tubes
Reservoirs, EIA Plates, and Additional Plastic Products

418
418
419
423
425

General Laboratory Equipment428

Plastic Consumables
Pipetsbio-rad.com/micropipets

Plastic Consumables
For liquid-handling applications, Bio-Rad offers pipets, pipet tips, reagent reservoirs, 96-well EIA plates, and tubes.

Pipets
A full set of high-precision micropipets is available for all your liquid handling needs.

Micropipets and Accessories
Professional Micropipets
These adjustable-volume digital pipets deliver
exceptional performance and are guaranteed to function
efficiently and reliably for years. Micropipets are available
in five volume ranges: 0.1–2.0, 0.5–10, 2–20, 20–200,
and 100–1,000 µl.
Micropipet features include:■

Adjustable digital dial with volume lock■

Improved ergonomic handle and tip ejector

Professional Micropipets

 ccommodation of standard pipet tips andAserological pipets

Professional Micropipet Specifications■■

3-year warranty*■

Fully autoclavable** Except pipet controller.

8-Channel Professional Micropipet
These multichannel micropipets are ideal for use withstandard 96-well formats. They have an adjustableworking volume range of 5–50 µl or 20–200 µl. A volume
lock feature will prevent any accidental volume changes.
Along with its ergonomic handle for right- and left-handuse, the manifold can rotate 360º for added pipetting
comfort. A curved ejector design pushes tips off in a single
step with minimal applied force.
Each channel has an independent precision piston assembly
to ensure accuracy and reproducibility from one pipetting
series to the next as well as between channels. Each pipet
has been tested and supplied with a certificate of quality anda calibration key. The multichannel pipet is fully autoclavable.
Professional Pipet Controller
This lightweight, ergonomic, cordless pipet controller
can be used with all plastic or glass pipets between
0.1 and 100 ml. Single-hand operation is easy with twobutton speed control for aspirating and dispensing. A large
LCD display clearly indicates battery status, pipet mode,and pipet speed. The unit comes with a bench stand,wall mount, charger, and 0.45 µm filter.

			AdjustableVolume		
VolumeRange, µl
Channels
Increments, µlP2

0.1–2.0

1

0.002

P10

0.5–10

1

0.02

P20

2–20

1

0.02

P200

20–200

1

0.2

P1000

100–1,00012.08-channel, P50

5–50

8

0.2

8-channel, P200

20–2008

0.2

8-Channel Professional
Micropipet

Pipet Controller

For More Information
Web: bio-rad.com/micropipets

418 | Plastic Consumables and General Laboratory Equipment

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables
bio-rad.com/pipettips

Pipet Tips

Professional Micropipet Set with Backpack
The professional micropipet set with backpack is a readyto-go, complete set for those who need to start up a newlab or refresh lab equipment to meet their pipetting needs.
This set comes with four professional micropipets (0.5–10,
2–20, 20–200, and 100–1,000 µl), a round carousel pipet
rack that can hold up to six single-channel micropipets,
four racks of Bio-Rad pipet tips (TBR-14, -35, -40, andProt/Elec) for general lab use, and a Bio-Rad backpack.

Carousel Pipet Rack

Carousel Pipet Rack
This round benchtop pipet rack holds up to six
single-channel pipets.

Professional Micropipet Set with Backpack

Ordering Information
Catalog #Description

1660499
1660505
1660506
1660507
1660508
1660496
1660495
1660486

Professional Micropipet, adjustable volume, 0.1–2.0 µl
Professional Micropipet, adjustable volume, 0.5–10 µl
Professional Micropipet, adjustable volume, 2–20 µl
Professional Micropipet, adjustable volume, 20–200 µl
Professional Micropipet, adjustable volume, 100–1,000 µl
Professional Micropipet, 8-channel, adjustable volume, 5–50 µl
Professional Micropipet, 8-channel, adjustable volume, 20–200 µl
Professional Micropipet Set with Backpack, set includes 4 professional series micropipets: P10, P20,
P200, P1000, carousel pipet rack, 4 racks of pipet tips (TBR-14, -35, -40, Prot/Elec), and Bio-Rad backpack
Professional Micropipet Prep Set, set of 3 Professional Adjustable-Volume Digital Micropipets: 2–20 µl,
20–200 µl, and 100–1,000 µl
Professional Micropipet Complete Set, set of 5 Professional Adjustable-Volume Digital Micropipets:
0.1-2 µl, 0.5–10 µl, 2–20 µl, 20–200 µl, and 100–1,000 µl
Professional Pipet Controller, 120 V, 0.1–100 ml, includes bench stand, charger, wall mount, 0.45 µm filter
Carousel Pipet Rack, holds 6 single-channel micropipets

17006704
17006703
1660490
1660487

Price, U.S. $337.50337.50337.50337.50337.50698.75698.75
1,198.75

373.75158.75

Pipet Tips
Bio-Rad’s pipet tips are made from virgin polypropylene
and have been accurately molded for an airtight fit.
The tips are guaranteed to have a smooth interior surface,which is essential for precision pipetting.
■ ips and racks are autoclavable at 120°C at 15 lb
Tof pressure for 15 min; tips presterilized by e-beamirradiation are also available

In the U.S., call toll free at 1-800-424-6723

■

■

■

 ll materials used in both clear and colored pipet tips
A
have been formulated without heavy metals
Xcluda pipet tips have been independently tested andcertified to be free of DNase, RNase, and pyrogens
 ipet tip racks are manufactured from polypropylene,Pwith a plastic content code of 5, and are free of labels forconvenient recycling

Plastic Consumables and General Laboratory Equipment | 419

Plastic Consumables
Pipet Tips

bio-rad.com/pipettips

Tip Selection Guide
Pipet TypeTip Type	Bio-Rad Professional
0.1–2.0 µl
0.5–10 µl
2–20 µl20–200 µl
				
100 –1,000 µl

Xcluda A, Seque/Pro, BR-14, TBR-14
Xcluda A, Seque/Pro, BR-31, TBR-14
Xcluda B, Prot/Elec, BR-35, TBR-35
Xcluda D, Prot/Elec, MTP-26, -35, -37, -38,
MTP-28-S, BR-35, -37, -38, -39, TBR-35
Xcluda H, BR-40, 41, TBR-40, -41

Costar (8-Pette, 12-Pette)
20–200 µl
28
25–200 µl
28
Eppendorf
0.5–10 µl
2–20 µl10–100 µl
50–250 µl
100–1,000 µl

Seque/Pro, Xcluda F, 31
Prot/Elec, Xcluda B, 14, 35, 37–39
Prot/Elec, Xcluda C and G, 35, 37–39
39, 40, 41
Xcluda H, 41

Excalibur
1–200 µl200–1,000 µl

Prot/Elec, 35, 37–39
40, 41	Thermo Labsystems Finnpipette
5–40 µl
Prot/Elec, Xcluda B, C, G, 35, 37–39
40–200 µl
Prot/Elec, Xcluda C and G, 35, 37–39
200–1,000 µl
Xcluda E

See Also
Thermal cyclers
for PCR:
page 364.
Real-time PCRsystems:
page 369.

Pipet TypeTip Type

Oxford Benchmate
0.5–10 µl
10–50 µl40–200 µl
200–1,000 µl

Seque/Pro, Xcluda A, 14
Xcluda J, 35, 37–39
Prot/Elec, Xcluda C, D, G, 35, 37–39
Xcluda E

Rainin Pipetman and EDP Series
0.1–10 µl
Seque/Pro, Xcluda F, 31
2–20 µl
Prot/Elec, Seque/Pro, Xcluda B, 31, 35, 37–39
10–100 µl
Prot/Elec, Xcluda C and G, 35, 37–39
20–200 µl
Prot/Elec, Xcluda D, 35, 37–39
100–1,000 µl
Xcluda E, 40, 41
Socorex0.5–10 µl
1–200 µl200–1,000 µl

Seque/Pro
Prot/Elec, 35, 37–39
40, 41

Titertek Flow
5–200 µl5–300 µl26
Xcluda D

Volac
1–20 µl1–200 µl200–1,000 µl

Prot/Elec, 31, 35, 37–39
Prot/Elec, 35, 37–39
40, 41

Xcluda Aerosol Barrier Pipet Tips
Xcluda aerosol barrier pipet tips guard against
aerosol contamination of samples, a feature of particularimportance in PCR experiments. The barrier will notseal on contact with liquid, which protects samples from
accidental loss, if unintentional overpipetting occurs.
Offered in nine different styles, the tips fit a variety of
pipets. They are available presterilized in fully enclosed
racks and are independently tested and certified
to be free of DNase, RNase, and pyrogens.
For More Information
Web: bio-rad.com/xcluda

A

B

C

D

E

F

G

H

J

Xcluda styles: A through J(shown at approximately
60% actual size)

Ordering Information
Catalog #Description

2112001
2112006
2112011211201621120212112026211203121120362112041
1660492

Xcluda Style A, 0.5–10 µl, 960
Xcluda Style B, 2–20 µl, 960
Xcluda Style C, 10–100 µl, 960
Xcluda Style D, 20–200 µl, 960
Xcluda Style E, 100–1,000 µl, 960
Xcluda Style F, 0.5–10 µl, 1,000
Xcluda Style G, 10–100 µl, 1,000
Xcluda Style H, 100–1,000 µl, 1,000
Xcluda Style J, 10–100 µl, 960
Professional Pipet Controller, 220 V for Australia, 0.1–100 ml, includes bench stand, charger, wall mount,0.45 µm filter
Carousel Pipet Rack, holds 6 single-channel micropipets

1660487

420 | Plastic Consumables and General Laboratory Equipment

Price, U.S. $180.00180.00180.00165.00165.00165.00165.00165.00160.00373.75158.75In the U.S., call toll free at 1-800-424-6723

Plastic Consumables
Pipet Tips

bio-rad.com/pipettips

See Also

Pipet Tips for Gel Loading

Vertical
electrophoresis
systems:
page 192.

Seque/Pro Capillary Pipet Tips
Seque/Pro capillary pipet tips have an average OD of<0.3 mm, making them ideal for loading sequencing gels
or IEF tube gels. Seque/Pro tips perform best when usedwith Eppendorf ultra micropipets (0.5–10 µl) and can also
be used with Rainin Pipetman 10 and 20 µl pipets.
Prot/Elec Pipet Tips
Prot/Elec tips fit easily within a gap of 0.75 mm between
vertical slab gel plates while maintaining a large bore forfast sample flow. The 200 µl capacity tips are molded to fit
Rainin Pipetman 20, 100, and 200 pipets and Eppendorf
20 and 100 µl pipets. Prot/Elec tips are also available with
an aerosol barrier to guard against airborne contaminants
and cross-contamination of samples (see Xcluda style G).

For More Information
Web: bio-rad.com/gelloading
Download bulletin: 5676

Ordering Information
Catalog #Description

2239911223991222399152239917
2239916

Seque/Pro Capillary Pipet Tips, in enclosed autoclavable rack, 0.5–10 µl, 200
Seque/Pro Capillary Pipet Tips, sterilized in enclosed rack, 0.5–10 µl, 200
Prot/Elec Pipet Tips, bulk pack, plastic bag in dust-free box, 1–200 µl, 1,000
Prot/Elec Pipet Tips, racked, 12 x 17 format, 204 per rack with a cover on each rack, 1–200 µl, 1,020
Prot/Elec Pipet Tips, racked, 8 x 12 format, 96 per rack with a cover on each rack, 1–200 µl, 960

Price, U.S. $
51.00
55.00
59.00
91.00
120.00See Also

Standard Pipet Tips
14

26

28

31

35

26

28

37

38

39

40

PCR tubes:
page 401.

41

For More Information
Web: bio-rad.com/standardtips
Tip Packaging Options
Standard Tip Type Number
Packaging Option

14

31

35

37

38

39

40

41

BR
•		•
•
•
•
•
•
•
•
RBR					•		 			
TBR•			•				•
•
MTP		
•			•
•
•			
MTP-S (sterilized)			
•		•
•
•			

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables and General Laboratory Equipment | 421

Plastic Consumables
Pipet Tips

bio-rad.com/pipettips

MTP
8 x 12 format, enclosed, nonflex racks are suitable for repeated reloading
and autoclaving. Racked tips are also available presterilized.
Fully enclosed
and autoclavable

Secure translucent cover

Nonflex tip
support surface
Integral bottom
and sides
Contrastingbackground for tips

Cover removal slot

8 x 12 multichannel
pipetting pattern

Frosted writing surfaces

BR
Bulk tips, plastic
bag in a dust-free
box.

RBR
Racked tips, one
cover on a stack ofnested racks.

TBR
Ideal for autoclaving,each rack has its
own lid and covered
bottom for full
enclosure.

Ordering Information
Catalog #Description

Price, U.S. $MTP 8 x 12 Format, Enclosed Racks
2239301
MTP-26 Tips, clear, 5–200 µl, 960
2239303
MTP-35 Tips, yellow, 1–200 µl, 960
2239304
MTP-37 Tips, clear, 1–200 µl, 960
2239313
MTP-38 Tips, yellow, graduated, beveled, 1–200 µl, 960

62.00
63.00
63.00
63.00

MTP-S 8 x 12 Format, Enclosed Racks, Presterilized*
2239307
MTP-28-S Tips, clear, 25–200 µl, 960
2239308
MTP-35-S Tips, yellow, 1–200 µl, 960
2239309
MTP-37-S Tips, clear, 1–200 µl, 960
2239318
MTP-38-S Tips, yellow, graduated, beveled, 1–200 µl, 960

76.00
76.00
76.00
76.00

BR Bulk Tips
2239014223902822390312239035
2239037
2239038223903922390402239041

38.00
38.00
38.00
38.00
38.00
38.00
38.00
19.00
19.00

BR-14 Tips, clear, 0.1–10 µl, 1,000
BR-28 Tips, clear, 25–200 µl, 1,000
BR-31 Tips, clear, 0.5–10 µl, 1,000
BR-35 Tips, yellow, 1–200 µl, 1,000
BR-37 Tips, clear, 1–200 µl, 1,000
BR-38 Tips, yellow, graduated, beveled, 1–200 µl, 1,000
BR-39 Tips, clear, graduated, beveled, 1–200 µl, 1,000
BR-40 Tips, blue, 100–1,000 µl, 500
BR-41 Tips, clear, 100–1,000 µl, 500

RBR Racked Tips, One Cover on Nested Racks
2239135
RBR-35 Tips, yellow, 1–200 µl, 1,000

49.00

TBR Racked Tips, Cover on Each Rack
2239354
TBR-14 Tips, clear, 0.1–10 µl, 1,000
2239347
TBR-35 Tips, yellow, 1–200 µl, 1,000
2239350
TBR-40 Tips, blue, 100–1,000 µl, 1,000
2239351
TBR-41 Tips, clear, 100–1,000 µl, 1,000

58.00
58.00
61.00
60.00

* Presterilized racks and polyethylene plugs are not suitable for autoclaving. Order unsterilized racks to reload tubes and autoclave.

422 | Plastic Consumables and General Laboratory Equipment

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables
Micro Test Tubes

bio-rad.com/microtesttube

Micro Test Tubes
Titertube Micro Test Tubes
Tubes and racks are made of polypropylene;
they are autoclavable to 120°C and freezable to –80°C.
The polyethylene plugs, which seal tightly for storage,
are not designed for repeated use and cannot be
autoclaved. The tubes are offered in sterile andunsterilized strips of eight, which may be cut toseparate the tubes. Titertube micro test tubes have:
■

■

■

■

1 ml capacity, 8.8 x 45 mm
Spacing that matches 96-well plates
Enclosed racks that stack to store 5,800 samples/ft3
Racks with a grid for identification of contents

For More Information
Web: bio-rad.com/titertube

Ordering Information
Catalog #Description

22393902239395*
223939122393932239392*2239394

Titertube Micro Test Tubes, unsterilized, 10 racks of 96 tubes
Titertube Micro Test Tubes, presterilized, 10 racks of 96 tubes
Titertube Micro Test Tubes, unsterilized, bulk, 1,000
Titertube Plugs, unsterilized, 960 in 120 strips of 8
Titertube Plugs, presterilized, 960 in 120 strips of 8
Titertube Rack, empty, holds 96 Titertube micro test tubes, 10

Price, U.S. $
51.00
58.00
39.00
19.00
24.00
15.00

* Presterilized racks and polyethylene plugs are not suitable for autoclaving. Order unsterilized racks to reload tubes and autoclave.

EZ Micro and Standard Micro Test Tubes
EZ Micro graduated polypropylene test tubes have afrosted marking area and a flat top that is pierceable by a
19 gauge or thicker needle. Standard polypropylene micro
test tubes are recommended for solvent extraction andheating when a very tight cap seal is required. Because of
their tighter cap fit, standard tubes may be more difficult tomanipulate in applications that require repeated opening
and closing of the tubes. Separate polyethylene caps for the
1.5 ml capless tubes have a knurled top for easy handling.
For More Information
Web: bio-rad.com/ezmicro
Download bulletin: 5676

500 µl EZ Micro

1.5 ml EZ MicroIn the U.S., call toll free at 1-800-424-6723

The micro test tubes are:■

Suitable for general-purpose benchtop centrifuge use;
sturdy uniform walls easily withstand up to 13,000 x g
■Autoclavable* to 120°C; freezable to –80°C■

Available in 2 ml, 1.5 ml, and 500 µl sizes* 1.5 ml colored microcentrifuge tubes are not autoclavable.

2 ml EZ Micro

1.5 ml Standard

1.5 ml Capless

Plastic Consumables and General Laboratory Equipment | 423

Plastic Consumables
Micro Test Tubes

bio-rad.com/microtesttube

Ordering Information
Catalog #Description

2239503
22394802239430*
2239501
2239500
2239490
1660473

EZ Micro Test Tubes, 500 µl, clear, 1,000
EZ Micro Test Tubes, 1.5 ml, clear, 500
EZ Micro Test Tubes, 2 ml, clear, 500
Micro Test Tubes, standard, 1.5 ml, clear, 500
Micro Test Tubes, capless, 1.5 ml, polypropylene, clear, graduated, 500
Separate Caps, for capless micro test tubes, white, 1,000
Colored Microcentrifuge Tubes, 1.5 ml, 6 colors, 600

Price, U.S. $
45.00
29.00
31.00
29.00
24.00
22.00
40.00

* To fit centrifuges, 2 ml tubes have thinner walls than standard tubes and are intended for short runs at less than 13,000 x g.
Heavy samples, high g forces, long runs, or centrifuges that radiate excessive heat may damage these tubes.

Screwcap Micro Test Tubes
Screwcap micro test tubes and caps are made ofpolypropylene. O-rings are made of a blend of polyethylene
and polypropylene. Features include:
■

■

■

■

O-ring operating range from –55 to 150°C
Uniform walls for uniform heat transfer
Conical bottoms for pellet collection
Knurled caps for easy handling

For More Information
Web: bio-rad.com/screwcap
Download bulletin: 5676

0.5 ml Conical

0.5 ml Skirted

1.5 ml Conical

2.0 ml Skirted

Screwcap

Ordering Information
Catalog #Description

2240165
2240185
2240100
2240110224013022401400.5 ml Conical Tubes, with installed O-ring screwcaps, sterilized, 500
0.5 ml Skirted Tubes, with installed O-ring screwcaps, sterilized, 500
1.5 ml Conical Tubes, with separate O-ring screwcaps, unsterilized, 500
1.5 ml Conical Tubes, with installed O-ring screwcaps, sterilized, 500
2.0 ml Skirted Tubes, with separate O-ring screwcaps, unsterilized, 500
2.0 ml Skirted Tubes, with installed O-ring screwcaps, sterilized, 500

424 | Plastic Consumables and General Laboratory Equipment

Price, U.S. $135.00135.0092.00
135.0092.00
135.00In the U.S., call toll free at 1-800-424-6723

Plastic Consumables
bio-rad.com/liquidhandlingReservoirs, EIA Plates, and Additional Plastic Products

Reservoirs, EIA Plates, and Additional Plastic Products
See Also

Reagent Reservoirs

PCR tubes:
page 401.

Disposable reagent reservoirs are presterilized andcompatible with multichannel pipets. Their sloping design
enables effective liquid pickup. The 50 ml capacity is
graduated to 25 ml in 5 ml increments.

ELISA reagents:
page 325.

For More Information
Web: bio-rad.com/reagentreservoirs

Reagent Reservoir

Ordering Information
Catalog #Description

2244872

Sterilized Reagent Reservoirs, graduated, polystyrene, 5 per package, box of 200

Price, U.S. $155.0096-Well EIA Plates
For EIAs, ELISAs, and other protein-binding assays,
use the Costar 96-well flat bottom EIA plate. This platebinds more protein more uniformly than any other plate.

Costar 96-Well
Flat Bottom EIA Plate

For More Information
Web: bio-rad.com/EIAplates

Ordering Information
Catalog #Description

2240096

Costar 96-Well Flat Bottom EIA Plates, polystyrene, 5 per package, box of 100

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $375.00

Plastic Consumables and General Laboratory Equipment | 425

Plastic Consumables
Reservoirs, EIA Plates, and Additional Plastic Products

See Also
Cuvettes:
page 31.

bio-rad.com/liquidhandling

Tube Racks and Storage Boxes
Bio-Rad plastic racks and storage units provide theultimate in ease of storage and sample organization.
Racks and Storage Box Selection Guide
Capacity

Dimensions (W x D x H)

Green racks

80 x 1.5/2.0 ml tubes

6.1 x 23.1 x 2.7 cm

Tube racks

60 x 15 ml
24 x 50 ml

10.5 x 24.6 x 7.2 cm
11.0 x 30.0 x 8.5 cm

Storage boxes

100 x 1.5/2.0 ml tubes

14.2 x 14.2 x 5.5 cm

Storage Boxes and Green Rack

For More Information
Web: bio-rad.com/racks

Tube Rack

Ordering Information
Catalog #Description

1660481
1660483
1660484
1660482

Green Racks, hold 80 x 1.5/2.0 ml tubes, set of 5Tube Racks, hold 60 x 15 ml tubes, set of 5Tube Racks, hold 24 x 50 ml tubes, set of 5
Storage Boxes, hold 100 x 1.5/2.0 ml tubes, set of 5, multicolored426 | Plastic Consumables and General Laboratory Equipment

Price, U.S. $
74.29
92.50
92.50
112.86In the U.S., call toll free at 1-800-424-6723

Plastic Consumables
bio-rad.com/plastics

Reservoirs, EIA Plates, and Additional Plastic Products

Additional Plastic Consumables
Bio-Rad now offers a wide selection of disposable plastic
components for your everyday cell/bacterial culturing usesin the lab.
■

■

■

■

■

■

■

 etri dishes – ready-to-use polystyrene cell andPbacterial culture petri dishes, 60 mm diameter, sterile
 el staining trays — disposable plastic trays ideal forGstaining mini gelsI noculation loops — the loops are smooth andflexible, making it easy to achieve uniform and smoothstreaking without damaging the agar surface. Using
disposable inoculation loops eliminates the risk of crosscontamination due to improper sterilization and the loops
do not need flaming, which reduces fire hazards in thelaboratory; 10 µl, sterile

Petri Dishes
Gel Staining Trays

15 ml Conical Centrifuge Tubes

 ellyfish foam floating racks — hold up to 12J
microcentrifuge tubes, for use in cold and hot water baths
 isposable plastic transfer pipets — polyethyleneDplastic pipet bulbs for liquid handling needs; sterile andnonsterile pipets are available onical centrifuge tubes — 15 ml polypropylene
C
conical tubes with volume graduations and screw caps

Jellyfish Foam Floating Rack
Disposable Plastic Transfer Pipets

 ell culture tubes — round bottom snap cap cultureC
tube, 14 ml, sterile
Inoculation Loops

Cell Culture Tubes

Ordering Information
Catalog #Description

1660476
1660475
1660470
1660471
1660474
1660480
1660477
1660479

Cell Culture Tubes, 17 x 100 mm, 14 ml, sterile, 25
Conical Centrifuge Tubes, 15 ml, 50
Petri Dishes, 60 mm diameter, sterile, 500
Inoculation Loops, 10 µl, sterile, 80
Disposable Plastic Transfer Pipets, sterile, 1 ml, graduated, 500
Disposable Plastic Transfer Pipets, nonsterile, 1 ml, graduated, 500
Gel Staining Trays, 4
Jellyfish Foam Floating Racks, 8

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $
22.50
33.75
161.2516.88
135.00
67.50
22.50
40.00

Plastic Consumables and General Laboratory Equipment | 427

General Laboratory Equipment
bio-rad.com/labequipment

General Laboratory Equipment
UView Mini Transilluminator

See Also
UView 6x loading
dye and stain:
page 265.

Visualize your gel using the compact UView minitransilluminator.
For More Information
Web: bio-rad.com/uview

Ordering Information
Catalog #Description

1660531

UView Mini Transilluminator

Price, U.S. $1,120.00

Centrifuges■■■

 ini centrifuge — includes microtube and PCR strip
M
tube rotors and 0.5/0.6 ml, 0.4/0.25 ml, and 0.2 ml tube
adaptors; the maximum speed is 6,000 rpm (2,000 x g).
Includes power adaptor for U.S./Canada, EU, UK,
and Australia
 odel 16K microcentrifuge — accommodates 1.5
M
or 2.0 ml tubes and has a quick-spin feature; themaximum speed is 14,000 rpm (16,000 x g). Safe forcoldroom operation
 CR tube adaptor for model 16K microcentrifuge —Pholds two PCR 8-tube strips or 16 individual
0.2 ml tubes

Model 16K Microcentrifuge(PCR tube adaptor sold separately)

Mini Centrifuge(includes PCR tube adaptor)

For More Information
Web: bio-rad.com/centrifuges

Ordering Information
Catalog #Description

12011919
1660602
16606121660620

Mini Centrifuge, 100–240 V
Model 16K Microcentrifuge, 120 V
Model 16K Microcentrifuge, 220 V
PCR Tube Adaptor, for model 16K microcentrifuge

428 | Plastic Consumables and General Laboratory Equipment

Price, U.S. $427.14
2,784.29
2,784.29
156.00In the U.S., call toll free at 1-800-424-6723

General Laboratory Equipment
bio-rad.com/labequipment

See Also

Mixing Devices
■

■

■

■

Micro test tubes:
page 423.

 ini rocker — provides superior three-dimensionalM
mixing action; gentle yet thorough rocking makes it ideal
to use for western blot incubations and gel staining.
Compact footprint requires minimal benchspace andenables it to fit inside our mini incubation oven
 ube roller — allows mixing of samples both horizontallyTand vertically. The compact design enables it to fit inside
our mini incubation oven, making it ideal for mixing liquids
while incubating. Comes with three interchangeabletube rotisseries to accommodate tubes from 1.5–50 ml.
Compact footprint requires minimal benchspace

PCR tubes:
page 401.

Mini Rocker

Tube Roller

BR-2000 vortexer — a general-purpose vortex mixer;
a flathead dimpled adaptor is also available
UltraRocker rocking platform — dual-platform rocker
that can be used for staining and destaining gels andblots, Southern hybridization, and overnight incubation

For More Information
Web: bio-rad.com/mixingdevices

BR-2000 Vortexer(flathead dimpled adaptor
sold separately)

UltraRocker Rocking Platform

Ordering Information
Catalog #Description

16607101660720166071116607211660722166061016606111660621
16606221660709
1660719

Mini Rocker, 120 V, includes two blotting boxes
Mini Rocker, 230 V (EU/UK), includes two blotting boxes
Tube Roller, 120 V, includes 3 tube carousels for 1.5, 15, and 50 ml tubes
Tube Roller, 230 V, includes 3 tube carousels for 1.5, 15, and 50 ml tubes
Tube Roller, 230 V (UK), includes 3 tube carousels for 1.5, 15, and 50 ml tubes
BR-2000 Vortexer, 120 V
BR-2000 Vortexer, 230 V
BR-2000 Vortexer, 230 V (UK)
Flathead Dimpled Adaptor, for BR-2000 vortexer
UltraRocker Rocking Platform, 120 V
UltraRocker Rocking Platform, 230 V

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables and General Laboratory Equipment | 429

General Laboratory Equipment
bio-rad.com/labequipment

Temperature Control Equipment■

Benchtop shaking incubator — handles small batch

culture needs easily, for example, growing bacteria or
yeast up to 4 L. The incubation temperature can be
set in 0.1° increments up to 65°C. The shaker speed isadjustable between 20 and 300 rpm for periods up to
99 hours or continuous operation. It features outstanding
temperature uniformity and stability. Secure a wide varietyof vessels with many available accessories■■■

 igital dry bath — digitally controlled dry bath that is idealD
for a multitude of laboratory procedures where incubation ofsamples is needed. It is accurate, built for safe continuous
operation, economical, and versatile. Comes with one
aluminum alloy 1.5 ml heating block (24 x 1.5 ml capacity).
Additional 0.5, 2.0, and 15 ml heating blocks with 24 x 0.5,
24 x 2.0, and 12 x 15 ml capacity, respectively, can bepurchased separately; 15 ml dry bath block not shown ini incubation oven — can accommodate up to eightyM
6.5 cm plates and operates at temperatures up to 60°C.
Contains a rear port to allow insertion of our tube roller ormini rocker for temperature-controlled mixing

Benchtop Shaking Incubator

Digital Dry Bath

0.5 mlDry Bath Block

2.0 mlDry Bath BlockWater bath — temperature controlled, dependable,
affordable water bath that includes stainless steel tankand lid, electrostatically applied finish that resists rust,
corrosion, and scratches, over-temperature protection,and thermometer

For More Information
Web: bio-rad.com/tempcontrol
Mini Incubation Oven

Water BathOrdering Information
Catalog #Description

17002944
17002945
17002946
17002947
1660562
1660563
1660565
1660566
1660567
1660501
1660504
166071216607131660570
1660571
1660572

Benchtop Shaking Incubator Starter Set, 120 V, includes 6 flask clamps
Benchtop Shaking Incubator Expanded Set, 120 V, includes 34 flask clamps and 1 petri dish shelf
Benchtop Shaking Incubator Starter Set, 230 V (EU/UK), includes 6 flask clamps
Benchtop Shaking Incubator Expanded Set, 230 V (EU/UK), includes 34 flask clamps and 1 petri dish shelf
Digital Dry Bath, 120 V, includes 1.5 ml heating block
Digital Dry Bath, 230 V (EU/UK), includes 1.5 ml heating block
Digital Dry Bath Heating Block, 0.5 ml, for 24 x 0.5 ml tubes
Digital Dry Bath Heating Block, 2.0 ml, for 24 x 2.0 ml tubes
Digital Dry Bath Heating Block, 15 ml, for 12 x 15 ml tubes
Mini Incubation Oven, 120 V
Temperature-Controlled Water Bath, 120 V
Mini Incubation Oven and Mini Rocker, 120 V
Mini Incubation Oven and Tube Roller, 120 V
Digital Dry Bath Heating Blocks, 3 pack, 0.5, 2.0, and 15 ml blocks
Digital Dry Bath with 4 heating Blocks, 115 V, includes 0.5, 1.5, 2.0, and 15 ml blocks, for the U.K. and Europe,
Digital Dry Bath with 4 Heating Blocks, 230 V, includes 0.5, 1.5, 2.0, and 15 ml blocks

430 | Plastic Consumables and General Laboratory Equipment

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables and General Laboratory Equipment
Subject Index

Subject Index
96-Well EIA Plates, 425
Additional Plastic Consumables, 427
Centrifuges, 428
EZ Micro and Standard Micro Test Tubes, 423
Micropipets and Accessories, 418
Mixing Devices, 429
Pipet Tips for Gel Loading, 421
Reagent Reservoirs, 425
Screwcap Micro Test Tubes, 424
Standard Pipet Tips, 421
Temperature Control Equipment, 430
Titertube Micro Test Tubes, 423
Tube Racks and Storage Boxes, 426
UView Transilluminator, 428
Xcluda Aerosol Barrier Pipet Tips, 420

In the U.S., call toll free at 1-800-424-6723

Plastic Consumables and General Laboratory Equipment | 431

432 | Plastic Consumables and General Laboratory Equipment

In the U.S., call toll free at 1-800-424-6723

Bio-Rad Explorer
Educational Products
Hands-On Inquiry-Based Science Education
Newest Products
Biotechnology Textbook
Advanced and Project-Based KitsClassroom Kits

434
434
438
439
442

Hands-On Inquiry-Based Science Education
Newest Products

bio-rad.com/explorer

Hands-On Inquiry-Based Science Education
Videos
Bio-Rad
Explorer Videos

bio-rad.com/
explorervideos

Do You Have Teaching Responsibilities?
BIO-RADThe Bio-Rad Explorer program makes it easy
for educators to meet AAAS and NSF’s goals for Vision
and Change in Undergraduate Biology Education.
The program provides kits, a textbook, and modular
lab series that bring project-based learning and researchskills into the classroom. Meet the challenge to:
■

■

■

TM

HANDS-ON INQUIRY-BASED SCIENCE EDUCATION

Integrate core concepts and competencies throughout
your curriculum
Focus on student-centered and project-based learning ngage the biology community in the implementationEof change

For more information about the products listed here,request the current Bio-Rad Explorer catalog (bulletin 2112)
or visit bio-rad.com/explorer.

Newest Products
New Virus Detection and Transmission Kit
A patient stumbles into a hospital with severe symptoms
associated with a newly emerging virus. Doctors diagnose
the infection while public health officials track down others
who might be infected. The individual was previously at arestaurant, but how did the virus spread?​
Based on published viral transmission case studies, thisactivity puts your students in the role of pathologist andepidemiologist as they analyze patient samples and use
patient information to determine how a virus spread througha restaurant.​
Use one of four included scenarios to keep students
engaged and to highlight important details of viral biology,
pathophysiology, molecular diagnostic techniques, andtransmission modes.​■ urriculum connections to virology, epidemiology,
C
respiratory and gastrointestinal functions and disease,
DNA structure, PCR, and gel electrophoresis■■

Choice of four different viral disease scenarios
 reamplified PCR DNA samples to teach PCR withoutPa thermal cycler

For More Information
Web: bio-rad.com/viruskit

Ordering Information
Catalog #Description

17008261EDU

Virus Detection and Transmission Kit, includes DNA samples, molecular weight ruler, loading dye, andmicrocentrifuge tubes; provides materials for 32 students or 8 workstations

17008241EDU

Virus Detection and Transmission Kit plus Fast Blast Electrophoresis Reagents, includes DNA
samples, molecular weight ruler, loading dye, Fast Blast DNA stain, agarose, TAE buffer, and microcentrifuge
tubes; provides materials for 32 students or 8 workstations

Inquire17008251EDU

Virus Detection and Transmission Kit plus Uview Electrophoresis Reagents, includes DNA samples,
molecular weight ruler, UView 6x loading dye and stain, agarose, TAE buffer, and microcentrifuge tubes;
provides materials for 32 students or 8 workstations

Inquire434 | Bio-Rad Explorer Educational Products

In the U.S., call toll free at 1-800-424-6723

Hands-On Inquiry-Based Science Education
Newest Products

bio-rad.com/explorer

Out of the Blue CRISPR and Genotyping Extension Kits
Perform real CRISPR gene editing in your laboratory class!
CRISPR gene editing is revolutionizing scientific research.
Now your students can do real CRISPR-Cas9 gene editingin the classroom using Bio-Rad’s educational CRISPR
gene editing kits.
■■ ut of the Blue CRISPR Kit — apply CRISPR-Cas9
O
technology to disrupt the lacZ gene in E. coli andproduce a phenotype that can be readily visualized
through blue-white screening ut of the Blue CRISPR Genotyping Extension —O(optional) confirm the edit at the molecular level using
multiplex PCR and agarose gel electrophoresis

Armed with their hands-on CRISPR gene editing
experience, your students then use bioinformatics
to explore the power and the limitations of this newtechnology and to debate the ethics of its use.

Genotype results(optional)

Use paper model tounderstand gene editing

Conduct bioinformatics
capstone activity

Edit lacZ gene





PA M

The Out of the Blue CRISPR and Genotyping Extension
kits earned the 2020 Science Educators’ Best of Show
award for Best New Technology Innovation for Sciencefrom the National Science Teaching Association (NSTA).

■

See our comprehensive award-winning teaching supports
at bio-rad.com/teachCRISPR.
■

 ands-on, true CRISPR gene editing — students getHdirect experience with this powerful technology
■

■

■

■

■

 isible phenotype — results can be observed with theVnaked eye in as little as 22 hr
 afe — familiar and common reagents and nonS
pathogenic, non-biofilm-forming bacteria generate aphenotype that is common to molecular biology
 ccessible — does not require specialized equipment
Aor techniques omprehensive, supportive curriculum — includes
Cbackground about the components of a CRISPR-Cas9
system, the science — and math — behind its accuracy
and flexibility, and the guided bioinformatic analysis of
CRISPR targets students design themselves
 esigned for use in the classroom, these activities canDbe completed in 50-minute class periods and use familiarand safe reagents, techniques, and organisms. If you
can perform a pGLO bacterial transformation in yourclassroom, you can do CRISPR gene editing!

For More Information
Web: bio-rad.com/outoftheblue

 ell-designed controls — demonstrate the role of eachW
component used in CRISPR-Cas9 gene editing toreinforce learning

Ordering Information
Catalog #Description

17006081EDU

 ut of the Blue CRISPR and Genotyping Extension Kits, CRISPR-Cas9 gene editing kit (12012608EDU) and genotypingOextension kit (120126078EDU) package. Requires electrophoresis reagents, sold separately. For educational use only — notfor self-administration

12012608EDU

 ut of the Blue CRISPR Kit, CRISPR-Cas9 gene editing kit, includes Refill Pack (12012620EDU) materials, LB agar andO
broth, petri dishes, inoculation loops, and microtubes. For educational use only — not for self-administration

12012607EDU

 ut of the Blue Genotyping Extension, CRISPR-Cas9 genotyping extension kit, includes Out of the Blue Extension Refill
O
Pack materials (12012708EDU), InstaGene matrix, and PCR, micro, and conical tubes. Requires Out of the Blue CRISPR kit(12012608EDU) and electrophoresis reagents, sold separately. For educational use only

In the U.S., call toll free at 1-800-424-6723

Price, U.S. $Bio-Rad Explorer Educational Products | 435

Hands-On Inquiry-Based Science Education
Newest Products

bio-rad.com/explorer

DNA Barcoding Kits
With our DNA Barcoding kits, students determine thespecies of fish, mammals, insects, or fungi based on the
DNA sequence of a standard reference gene (cytochromec oxidase I (COI) or the internal transcribed spacer (ITS).
Students extract DNA from tissue, amplify the gene by
PCR, verify PCR products by agarose gel electrophoresis,sequence the gene, and then use the sequence obtained
and bioinformatics to genetically identify the species.
The unique sequence produced for a given sample isconsidered to be its DNA barcode.
Kits and refill modules are available for the investigation offish, mammalian, insect, and fungal samples.
■

■

■

■

 erform DNA extraction, PCR amplification, and gelPelectrophoresis
 se DNA sequencing and bioinformatics to determineUspecies identity
 xamine biodiversity or see if marketplace substitution isE
occurringContribute to global initiatives for barcoding life

For More Information
Web: bio-rad.com/barcoding

Ordering Information
Catalog #Description

12012608EDU

Fish DNA Barcoding Kit, PCR and bioinformatics kit, includes DNA Extraction Module (12016408EDU)
and Fish PCR Module (12016300EDU); for extraction and PCR amplification of up to 16 fish DNA andcontrol samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation); foreducational use only

17007366EDU

Mammals, Insects, and Fungi DNA Barcoding Kit, PCR and bioinformatics kit, includes DNA Extraction
Module (12016408EDU) and Mammals, Insects, and Fungi PCR Module (12016353EDU); for extraction and
PCR amplification of up to 16 DNA and control samples (8 student workstations, 2 samples per workstation,
2–4 students per workstation); for educational use only

17007154EDU

Fish, Mammals, Insects, and Fungi DNA Barcoding Kit, PCR and bioinformatics kit, includes two DNA
Extraction Modules (12016408EDU), one Fish PCR Module (12016300EDU), and one Mammals, Insects, andFungi PCR Module (12016353EDU); for educational use only

12016408EDU

DNA Extraction Module, reagents and plastic consumables for chromosomal DNA extraction, includes
resuspension, lysis, and neutralization solutions, wash buffer, matrix, spin filters, and 2.0 ml microtubes for
up to 16 extractions; for educational use only

12016300EDU

Fish PCR Module, reagents and consumables for PCR amplification of up to 16 fish DNA samples andcontrol DNA, contains 2x master mix, PCR primers, control DNA, sterile water, molecular weight ruler,
fluorescent DNA loading dye and stain, and PCR tubes; for educational use only

12016353EDU

Mammals, Insects, and Fungi PCR Module, reagents and consumables for PCR amplification of up to 16
DNA samples and control DNA, contains 2x master mix, PCR primers, control DNA, sterile water, molecularweight ruler, fluorescent DNA loading dye and stain, and PCR tubes; for educational use only

436 | Bio-Rad Explorer Educational Products

In the U.S., call toll free at 1-800-424-6723

Hands-On Inquiry-Based Science Education
bio-rad.com/explorer

Newest Products

pGLO Bacterial Transformation Kit for General Biology
This scaffolded approach to the classic pGLO bacterialtransformation lab teaches core genetics concepts —including gene expression and gene regulation — using
a student-driven experimental design experience. First,
students use bacterial transformation to investigate therole of antibiotic selection markers. Next, they design andconduct another experiment to probe gene regulation as
they switch on expression of green fluorescent protein(GFP) in the transformed bacteria. Finally, they apply what
they’ve learned to design a biosensor, based on bacterialtransformation, to solve a real-world problem.
■

■

■

■

Includes consumables and reagents for 12 laboratory
workstations or 48 students
 nables students to design and analyze two geneEexpression experiments
 llows students to create and revise models to explainA
their observations
Activities designed for three to five 50-minute class periods

For More Information
Web: bio-rad.com/pglogenbiokit

Ordering Information
Catalog #Description

17006991EDU

pGLO Bacterial Transformation Kit for General Biology, includes pGLO plasmid, nutrient broth/powder,
petri dishes, UV pen light, transformation reagents, and consumables for 48 students or 12 workstations

In the U.S., call toll free at 1-800-424-6723

Bio-Rad Explorer Educational Products | 437

Hands-On Inquiry-Based Science Education
Biotechnology Textbook

bio-rad.com/explorer

Biotechnology Textbook
Biotechnology: A Laboratory Skills Course, Second Edition
This laboratory textbook blends textbook theory withhands-on laboratory activities and real-world applications
for your biotechnology course, and it incorporates
Bio-Rad Explorer kits for easy implementation supported bylive technical support. This textbook encourages the next
generation of biotechnologists by:
■

■

■

■

Developing key skills with multiple activities
 ncouraging students to consider the broaderE
implications of biotechnology with bioethics case studies
 roadening occupational awareness with profiles ofBcareers in biotech
 etting students answer a research question usingL
independent research

The teacher supplement provides a thorough backgroundon preparation, setup, results analysis, and assessment.
It also provides guidance on how to implement and build
your biotechnology course. Chapters include:
■

■

■

■

■

■

■

■

■

The Biotechnology Industry
Laboratory Skills
Microbiology and Cell Culture
DNA Structure and Analysis
Bacterial Transformation and Plasmid Purification
Polymerase Chain Reaction
Protein Structure and Analysis
Immunological Applications
Research Projects

For More Information
Web: bio-rad.com/textbook

Ordering Information
Catalog #Description

17004717EDU

Biotechnology: A Laboratory Skills Course, Second Edition, teacher edition, includes
one student edition and one teacher supplement
Biotechnology: A Laboratory Skills Course, Second Edition, student edition
Biotechnology: A Laboratory Skills Course, Second Edition, teacher supplement
Laboratory Notebook, one notebook
Laboratory Notebook, classroom set of 32

12008528EDU
12008527EDU
1661051EDU
1661053EDU

Price, U.S. $150.00117.50
43.75
18.75
18.75

EDU price discounts are for qualified educational institutions and educators only. Items are available at list pricefor noneducators (must be ordered without an EDU suffix).

438 | Bio-Rad Explorer Educational Products

In the U.S., call toll free at 1-800-424-6723

Hands-On Inquiry-Based Science Education
Advanced and Project-Based Kits

bio-rad.com/explorer

Advanced and Project-Based Kits
Modular Kits for Complete Laboratory Workflows
Integrated College Level Molecular Biology Labs
Looking for authentic project-based learning lab
experiences that carry a gene or protein of interest fromisolation to analysis?
Bio-Rad’s modular lab series provides validated
procedures, easy preparation, and reproducible successyear after year. Visit bio-rad.com/explorer to learn
about our advanced series for cloning, sequencing,bioinformatics, and protein expression and purification
using affinity chromatography. These flexible, modular lab
series can be used as capstone projects or a complete
molecular biology course.

Cloning and Sequencing
Explorer Series*

Protein Expression and
Purification Series*

1. Nucleic Acid Extraction
1. Growth and Expression
2. SDS-PAGE Electrophoresis
2. GAPDH PCR
3. Electrophoresis
3. Purification Process Options:
4. PCR Kleen Spin Purification		 Centrifugation Purification
5. Ligation and Transformation		 Hand-Packed Column Purification
6. Microbial Culturing		 Prepacked Cartridge Purification
4. DHFR Enzymatic Assay
7. Aurum Plasmid Mini Purification
5. Assessment
8. Sequencing and Bioinformatics* Available as a complete series or as individual modules.
For More Information
Web: bio-rad.com/cloninglab
bio-rad.com/proteinpurificationlab

Protein Expression
and Purification Series

Cloning and Sequencing
Explorer Series
NOW INCLUDES

Ordering Information
Catalog #Description

Price, U.S. $Cloning and Sequencing Explorer Series
1665000EDU*
1665005EDU*
1665010EDU*
1660451EDU
7326300EDU
1665015EDU*
1665020EDU
7326400EDU
1665025EDU*

Complete Cloning and Sequencing Explorer Series, includes all 8 modules (sequencing service
not included)
Nucleic Acid Extraction Module
GAPDH PCR Module
Electrophoresis Module
PCR Kleen Spin Purification Module
Ligation and Transformation Module
Microbial Culturing Module
Aurum Plasmid Mini Purification Module
Sequencing and Bioinformatics Module, includes sequencing primers, control plasmid,and bioinformatics subscription; sequencing service not included

1,906.25
215.00498.75133.3378.75
406.25121.25217.14327.50continues

In the U.S., call toll free at 1-800-424-6723

Bio-Rad Explorer Educational Products | 439

Hands-On Inquiry-Based Science Education
Advanced and Project-Based Kits

bio-rad.com/explorer

Ordering Information
Catalog #Description

Price, U.S. $Protein Expression and Purification Series
1665040EDU* Protein Expression and Purification Series, centrifugation purification process
1665045EDU* Protein Expression and Purification Series, hand-packed purification process
1665050EDU* Protein Expression and Purification Series, prepacked purification process
Protein Expression and Purification Series Assessment Module, formative and summative
1665070EDU
assessment tool
1665055EDU* Growth and Expression Module
1665060EDU* SDS-PAGE Electrophoresis Module
1665041EDU
Centrifugation Purification Module
1665046EDU
Hand-Packed Purification Module
1665051EDU
Prepacked Purification Module
1665065EDU* DHFR Enzymatic Assay Module723.75916.25916.2563.75
317.50223.7570.00
262.50262.50113.75* Ships with both temperature-sensitive and room temperature components. Immediately store temperature-sensitive items
at 4°C or –20°C as indicated.
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price fornoneducators (must be ordered without an EDU suffix).

Stain-Free Western Blotting Workflow (Stain-Free Rapid Blotting)Western Blotting in Less than 2 Hours!
The rapid blotting, or Stain-Free western blotting,workflow allows you to complete an entire western blot
workflow in less than 2 hours in the classroom.
TGX Stain-Free gels combined with the super-fast
Trans-Blot Turbo transfer system provide maximum timesavings, allowing you to complete the workflow in asingle lab block period. Teach students about the exciting
chemistry that allows visualization of samples separated
on PAGE gels without staining.

Hands-On Time Expenditure (in minutes)

Protein extractionand electrophoresis

33

33

33

For More Information
Web: bio-rad.com/EDU/rapidblotting

Protein visualization

			
			
Rapid
			
blotting		Tank(staining		blotting
required)

Stain-Free
western blotting
workflow(Stain-Free
rapid blotting)

180

180

<3

Protein transfer
Equilibration
Setup
Transfer

15
30
30–150

0
5
15

0
5
15

Immunoblotting

45

45

45

Color detection		All options: 10 min to overnight
Total hands-on time				

343–463(5 hr 43 min
to 7 hr 43 min)288
(4 hr 48 min)111
(1 hr 51 min)Total time savings of 55 min to 7 1/2 hr

Ordering Information
Catalog #Description

Price, U.S. $1662875EDU

Rapid Blotting and Stain-Free Western Blotting Starter Kit, comprehensive protein classroom study kit,includes protein profiler module (#1662700EDU), western blot module (#1662800EDU), Trans-Blot Turbomini nitrocellulose transfer pack, TGX Stain-Free gels, application note, for 32 students

717.50EDU price discounts are for qualified educational institutions and educators only. Items are available at list price fornoneducators (must be ordered without an EDU suffix).

440 | Bio-Rad Explorer Educational Products

In the U.S., call toll free at 1-800-424-6723

Hands-On Inquiry-Based Science Education
bio-rad.com/explorer

Advanced and Project-Based Kits

Real-Time PCR Kits
How Much DNA Is There?
The use of real-time PCR in research and diagnostics is
accelerating as it offers a streamlined, sensitive approachto determining levels of pathogens in tissues and inwastewater, detecting aneuploidies, diagnosing othergenetic diseases, and monitoring microbial contaminantsin environmental samples, among many other applications.
Teaching students this robust technology prepares themfor a future in all areas of life science — basic research,biotechnology, medicine, food analysis, diagnostics, andmore.
Teach the principles and techniques of real-time PCR:■■■ xamine the impact of pipetting and other factors onEsensitivity and accuracy
 emonstrate standard curve generation and meltD
curve analysis
Illustrate the unique benefits and applications of bothreal-time PCR and conventional, endpoint PCR

Crime Scene Investigator PCR Basics Real-Time
PCR Starter Kit
An excellent starting point for novices, this kit uses premade
DNA samples to teach the basics of real-time PCR. Thiseliminates the variables associated with DNA extraction andsample preparation. Additionally, DNA fingerprints can still

be investigated using gel electrophoresis and melt curve
analysis, showing how real-time and standard PCR can becomplementary techniques.
GMO Investigator Real-Time PCR Starter Kit
Teach the entire real-time PCR workflow, from DNA
extraction from food items to data analysis, as you quantify
the amount of genetically modified ingredients.
For More Information
Web: bio-rad.com/EDU/real-time
Download bulletin: 3332

Ordering Information
Catalog #Description

1662660EDU

Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit, introductory real-time PCR classroom
study kit, contains Crime Scene Investigator PCR Basics kit (1662600EDU) and additional real-time reagentsfor 32 students or 8 workstations
GMO Investigator Real-Time PCR Starter Kit, introductory real-time PCR classroom study kit, contains
GMO Investigator Kit (1662500EDU) and additional real-time reagents for 32 students or 8 workstations

1662560EDU

Price, U.S. $690.00700.00EDU price discounts are for qualified educational institutions and educators only. Items are available at list price fornoneducators (must be ordered without an EDU suffix).

In the U.S., call toll free at 1-800-424-6723

Bio-Rad Explorer Educational Products | 441

Hands-On Inquiry-Based Science Education
Classroom Kits

bio-rad.com/explorer

Classroom Kits
Bio-Rad Explorer Kits
Bio-Rad Explorer kits address the critical need for inquirybased activities — an important component of scientific
literacy for an educated citizenry and a launch point ofexperience and practical training for students interested
in careers in life science. The kits range from introductory
to advanced topics, including courses guiding students
through entire molecular biology workflows. ThINQ!
Investigation kits encourage open inquiry in the classroom.
Areas of biotechnology applications covered include:■

Bacterial transformation and microbiology■

CRISPR gene editing■

Protein analysis and chromatography■

DNA analysis■

PCR amplification■

 ully developed course series in DNA and proteinF(see page 439)

For a complete product listing, please refer to the
Bio-Rad Life Science Education Catalog (bulletin 2112)
or visit bio-rad.com/explorer.

Ordering Information
Catalog #Description

Introductory Kits
17005278EDU
Engineering Solutions for Global Health Kit, provides materials for 96 students or 24 workstations
1660003EDU*
pGLO Bacterial Transformation Kit, provides materials for 32 students or 8 workstations
17006991EDU* pGLO Bacterial Transformation Kit for General Biology, provides materials for 48 students or 12 workstations
1662300EDU
Genes in a Bottle Kit, includes 1 DNA extraction module (#1662000EDU) and DNA necklace module(#1662250EDU); provides materials for 36 students or 9 workstations
1665075EDU
1665090EDU
1662900EDU*
1665030EDU
1660008EDU
1662400EDU*IDEA Kit — Inquiry Dye Electrophoresis Activity, provides materials for 32 students or 8 workstations
STEM Electrophoresis Classroom Kit, provides materials for 32 students or 8 workstations
Got Protein? Kit, provides materials for 320 students or 80 workstations
Microbes and Health Kit, provides materials for 32 students or 8 workstations
Size Exclusion Chromatography Kit, provides materials for 32 students or 8 workstations
ELISA Immuno Explorer Kit, provides materials for 48 students or 12 workstations

Intermediate Kits
1665120EDU
C. elegans Behavior Kit, provides materials for 32 students or 8 workstations
1665035EDU*
Biofuel Enzyme Kit, provides materials for 32 students or 8 workstations
1660007EDU*
Forensic DNA Fingerprinting Kit, provides materials for 32 students or 8 workstations
1660013EDU*
pGLO Kit SDS-PAGE Extension, provides materials for 32 students or 8 workstations
1660005EDU*
Green Fluorescent Protein (GFP) Chromatography Kit, provides materials for 32 students or 8 workstations
1660006EDU*
Secrets of the Rainforest Kit, provides materials for 32 students or 8 workstations
1660001EDU*
Analysis of Precut Lambda DNA Kit, provides materials for 32 students or 8 workstations
1660002EDU*
Restriction Digestion and Analysis of Lambda DNA Kit, provides materials for 32 students or 8 workstations
1662700EDU*
Comparative Proteomics Kit I: Protein Profiler Module, provides materials for 32 students or 8 workstations
17005316EDU
Science of Opioid Dependence Kit, provides materials for 32 students or 8 workstations
17008261EDU
Virus Disease Detection and Transmission Kit, provides materials for 32 students or 8 workstations
continues

442 | Bio-Rad Explorer Educational Products

In the U.S., call toll free at 1-800-424-6723

Hands-On Inquiry-Based Science Education
Classroom Kits

bio-rad.com/explorer

Ordering Information
Catalog #Description

Price, U.S. $Advanced Kits
12012608EDU* Out of the Blue CRISPR Kit, provides materials for 32 students or 8 workstations
12012607EDU* Out of the Blue Genotyping Extension, provides materials for 32 students or 8 workstations
17007154EDU* Fish, Mammals, Insects, and Fungi DNA Barcoding Kit, provides materials for 32 students or 8 workstations
1662800EDU*
Comparative Proteomics Kit II: Western Blot Module, provides materials for 32 students or 8 workstations
1662600EDU*
Crime Scene Investigator PCR Basics Kit, provides materials for 32 students or 8 workstations
1662100EDU*
PV92 PCR Informatics Kit, provides materials for 32 students or 8 workstations
1662500EDU*
GMO Investigator Kit, provides materials for 32 students or 8 workstations
1662875EDU

Rapid Blotting and Stain-Free Western Blotting Starter Kit, provides materials for 32 students or 8 workstations

1662560EDUGMO Real-Time PCR Starter Kit, provides materials for 32 students or 8 workstations

1662660EDU

Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit, provides materials for 32 students
or 8 workstations

1665000EDU

Cloning and Sequencing Explorer Series, 8 modular activities

1665040EDU

Protein Expression and Purification Series — Centrifugation Purification Process

1665045EDU

Protein Expression and Purification Series — Hand-Packed Purification Process

1665050EDU

Protein Expression and Purification Series — Prepacked Purification Process

248.75220.00262.50233.75

ThINQ! Investigation Kits
17001238EDU Photosynthesis and Cellular Respiration Kit for AP Biology, provides materials for 32 students or 8 workstations
12005534EDU

Photosynthesis and Cellular Respiration Kit for General Biology, provides materials for 96 students or
24 workstations

1660335EDU

pGLO Transformation and Inquiry Kit for AP Biology, provides materials for 32 students or 8 workstations

17001235EDU

Biofuel Enzyme Reactions Kit for AP Biology, provides materials for 32 students or 8 workstations

17002878EDU

Giant Panda Problem Kit for AP Biology, provides materials for 32 students or 8 workstations* Ships with both temperature-sensitive and room temperature components. Immediately store temperature-sensitive items
at 4°C or –20°C as indicated.
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price fornoneducators (must be ordered without an EDU suffix).

In the U.S., call toll free at 1-800-424-6723

Bio-Rad Explorer Educational Products | 443

Bio-Rad Explorer Educational Products
Subject Index

bio-rad.com/explorer

Biotechnology: A Laboratory Skills Course, Second Edition 438
Bio-Rad Explorer Kits, 442
DNA Barcoding Kits, 436
Modular Kits for Complete Laboratory Workflows, 439
Out of the Blue CRISPR and Genotyping Extension Kits, 435
pGLO Bacterial Transformation Kit for General Biology, 437
Real-Time PCR Kits, 441
Stain-Free Western Blotting Workflow (Stain-Free Rapid Blotting), 440
Virus Detection and Transmission Kit, 434

444 | Bio-Rad Explorer Educational Products

In the U.S., call toll free at 1-800-424-6723

AppendicesTrademarks

446

Legal Notices

447

References448

Trademarks and Legal Notices
bio-rad.com

Trademarks
The following are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions.
AFFI-GELAFFI-PREPAGAMINEX
BIO-SPINAMPLISIZEAUTODG
BIO-DOTBIO-GEL
BIO-LYTE
BIO-PLEXBIO-RADDDPCR
CELSELECT SLIDESCHELEXCHTDDSEQ
DROPLET DIGITALECONO-COLUMNECONO-PAC
GENE PULSER
HARD-SHELLHELIOSIMMUN-BLOT
MACRO-PREP
MICROSEALMINI TRANS-BLOT
MINI-PROTEANMINI-SUB
POLY-PREP

PRECISIONAB
PREVALENCE
PROTEANQUANTASOFTQUANTUM PREP
QX200
QX600
QX ONESEQUOIASTARBRIGHTSUB-CELL
TITERTUBE
TRANS-BLOTUNO

The following is a trademark of Bio-Rad Europe GmbH in certain jurisdictions.
LYNX RAPID CONJUGATION KIT
All trademarks used herein are the property of their respective owner.

446 | Appendices and Index

In the U.S., call toll free at 1-800-424-6723

Trademarks and Legal Notices
bio-rad.com

Legal Notices
The Bio-Plex suspension array system includes
fluorescently labeled microspheres and instrumentation
licensed to Bio-Rad Laboratories, Inc. by the
Luminex Corporation.
Clarity Max western ECL substrate is manufactured byCyanagen Srl and is the subject of U.S. and ex-U.S.
patents, including US7855287, EP1950207, US9040252,
AU2011202658, CA2742025, US8129136, EP1962095 andCN102313732.
Nuvia aPrime 4A Resin is covered by U.S. Patent Number
9,669,402 and foreign counterparts.
CysDisplay, HuCAL, and HuCAL PLATINUM are
trademarks of MorphoSys AG in certain jurisdictions.
Products made using HuCAL technology and/or their use
are covered by the following U.S. patents and/or pending
U.S. patent application or their foreign counterparts
owned by or under license to Bio-Rad Laboratories, Inc.,
including, but not limited to, U.S. Patent No. 8,273,688.
Products containing SpyTag1, SpyTag3, SpyCatcher1 or
SpyCatcher3 and/or their use are covered by the following
U.S. patents and/or pending U.S. patent application or
their foreign counterparts owned by or under license to
Bio-Rad Laboratories, Inc., including, but not limited to,
U.S. Patent Nos. 9,547,003, 10,247,727, and 10,527,609.
Products containing SpyTag2 or SpyCatcher2 and/or their use are covered by the following U.S. patents
and/or pending U.S. patent application or their foreigncounterparts owned by or under license to Bio-Rad
Laboratories, Inc., including, but not limited to, U.S. PatentNos. 9,547,003, 10,247,727, 10,527,609, and 11,059,867.
SpyDisplay technology is covered by U.S. patent no.
11,453,883 and pending ex-U.S. counterparts.
Purchase of Digital PCR and/or Single-Cell NGS
Sample Preparation products (the “Products”)
from Bio-Rad Laboratories is subject toBio-Rad Laboratories, Inc. Standard Terms
and Conditions of Sale, which can be accessed athttps://www.bio-rad.com/en-us/terms-conditions.
Unless we expressly state otherwise in additional
Terms and Conditions, no rights are granted for you
to distribute or resell the Products. Unless we
expressly state otherwise in additional Terms andConditions, no rights are granted for the development
or commercialization of diagnostic assays for use with
the Products without a license from Bio-Rad. The
Products and/or their use are covered by U.S. andforeign patents and/or pending patent applications
owned by or under license to Bio-Rad Laboratories, Inc.
See https://www.bio-rad.com/en-us/trademarks.

In the U.S., call toll free at 1-800-424-6723

The QX ONE/QX600/QX200/QX100 Droplet Digital PCR
Systems, and the consumables and reagents designed towork with these systems, and/or their use is covered byclaims of U.S. patents and/or pending U.S. and non-U.S.
patent applications owned by or under license to Bio-Rad
Laboratories, Inc. See bio-rad.com/en-us/trademarks fordetails. Purchase of the product includes a limited, nontransferable right under such intellectual property for useof the product for internal research purposes in the field ofdigital PCR only. No rights are granted for diagnostic uses.
No rights are granted for use of the product for commercial
applications of any kind, including but not limited to
manufacturing, quality control, or commercial services,such as contract services or fee for services. Information
concerning a license for such uses can be obtained
from Bio-Rad Laboratories. It is the responsibility of thepurchaser/end user to acquire any additional intellectual
property rights that may be required.
Notice regarding high resolution melt analysis: No rights
are granted by Bio-Rad for the use of high resolution
melt analysis in the fields of human or veterinary in vitro
diagnostics. In addition, it is the responsibility of thepurchaser to obtain any intellectual property rights which
may be required for its specific applications.
SYBR is a trademark of Thermo Fisher Scientific Inc.
Bio-Rad Laboratories, Inc. is licensed by Thermo Fisher
Scientific Inc. to sell reagents containing SYBR Green I for
use in real-time PCR, for research purposes only.
EvaGreen is a trademark of Biotium, Inc. Bio-Rad
Laboratories, Inc. is licensed by Biotium, Inc. to sell
reagents containing EvaGreen dye for use in real-time PCR,
for research purposes only.
TGX Stain-Free precast gels are covered by U.S. Patent
Numbers 7,569,130 and 8,007,646.
The Mini-Sub Cell GT, Wide Mini-Sub Cell GT, and SubCell GT Cell Systems and/or their use are covered by thefollowing U.S. patent and/or pending U.S. patent applicationor their foreign counterparts owned by or under license to
Bio-Rad Laboratories, Inc., including, but not limited to, U.S.
Patent No. 8,262,889.
Image Lab Touch software is based in part on the work of
the CImg project (http://cimg.eu/). See license for details at“http://www.cecill.info/licences/Licence_CeCILL-C_V1-en.
html”www.cecill.info/licences/Licence_CeCILL-C_V1-en.
html. Image Lab Touch is based in part on libraries from
GCC runtime and the Gnu C library.
SureCell and the corresponding logos are trademarks ofIllumina, Inc.
xMAP and Luminex are trademarks of Luminex Corporation.

Appendices and Index | 447

References
bio-rad.com

References
Sample Preparation

Electrophoresis and Blotting

Chomczynski P and Sacchi N (1987). Single-step methodof RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 162, 156–159.

Gatti RA et al. (1984). Multiple uses of Southern blots.
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Sample Quantitation
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Merril CR et al. (1981). A rapid silver stain for polypeptides
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448 | Appendices and Index

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