Lonza Rockland, Inc.
www.lonza.com
biotechserv@lonza.com
Tech Service: 800-521-0390
Customer Service: 800-638-8174
Document # 18100-0807-8
Rockland, ME 04841 USASeaKem® LE Agarose
The first and finest name in agarose.

Introduction
SeaKem® LE Agarose is an all purpose agarose for routine nucleic acid electrophoresis of fragments between
500bp-23,000 bp. SeaKem® LE Agarose has nodetectable DNase or RNase activity.
Analytical Specifications
Gelling temperature (1.5%)
Melting temperature (1.5%)
Gel strength (1%)

Microwave Instructions for Agarose Preparation

36°C ±1.5°C>90°C>1,200 g/cm²

1.

Applications• Analytical electrophoresis of DNA and RNA >1,000 bp• Blotting of DNA and RNA
Suggested Agarose Concentrations
Size Range

Final Agarose Concentration (%)(Base Pairs)

1X TAE Buffer

1X TBE Buffer

1,000-23,000
800-10,000
400-8,000
300-7,000
200-4,000
100-3,000

0.60
0.80
1.00
1.20
1.50
2.00

0.50
0.70
0.85
1.00
1.25
1.75

Dye Mobility Table
Migration of double-stranded DNA in relation toBromophenol Blue (BPB) and Xylene Cyanol (XC) inSeaKem® LE Agarose Gels.
1X TAE Buffer
XC

24,80011,000
10,200
6,100
3,560
2,800
1,800
1,300

BPB

2,900
1,650
1,000
500
370
300
200
150%
Agarose

0.30
0.50
0.75
1.00
1.25
1.50
1.75
2.00

1X TBE Buffer
XC

19,40012,000
9,200
4,100
2,500
1,800
1,100
850

Precautions
Always wear eye protection when dissolving agaroseand guard yourself and others against scaldingsolutions. Refer to Material Safety Data Sheet for
additional safety and handling information.

BPB

2,850
1,350
720
400
260
200
110
70

Choose a beaker that is 2-4 times the volume of thesolution.
2. Add room temperature 1X or 0.5X electrophoresis
buffer and a stir bar to the beaker.
3. Slowly sprinkle in the agarose powder while thesolution is rapidly stirred.
4. Remove the stir bar if not Teflon® coated.
5. Weigh the beaker and solution before heating.
6. Cover the beaker with plastic wrap.
7. Pierce a small hole in the plastic wrap for ventilation.
8. Heat the beaker in the microwave oven on Highpower until bubbles appear.
9. Remove the beaker from the microwave oven.
Caution: Any microwaved solution may
become superheated and foam over whenagitated.
10. GENTLY swirl the beaker to resuspend any settled
powder and gel pieces.
11. Reheat the beaker on HIGH power until the solution
comes to a boil.
12. Hold at boiling point for 1 minute or until all of theparticles are dissolved.
13. Remove the beaker from the microwave oven.
14. GENTLY swirl the beaker to thoroughly mix theagarose solution.
15. After dissolution, add sufficient hot distilled water toobtain the initial weight.
16. Mix thoroughly.
17. Cool the solution to 50°C-60°C prior to casting.

Hot Plate Instructions for Agarose Preparation
1. Choose a beaker that is 2-4 times the volume of thesolution.
2. Add room-temperature electrophoresis buffer and astir bar to the beaker.
3. Slowly sprinkle the agarose powder while thesolution is rapidly stirred.
4. Weigh the beaker and solution before heating.
5. Cover the beaker with plastic wrap.
6. Pierce a small hole in the plastic wrap for ventilation.
7. Bring the solution to a boil while stirring.
8. Maintain gentle boiling until all the agarose isdissolved (approximately 10 minutes).
9. Add sufficient hot distilled water to obtain the initialweight.
10. Mix thoroughly.
11. Cool the solution to 50°C-60°C prior to casting.
Ordering Information:
Catalog No.
50001
50002
50000
50004

Size25 g
100 g
125 g
500 g

For more information on SeaKem® LE Agarose, contact
Technical Service at (800) 521-0390 or visit our website
at www.Lonza.com.
Related Products:
DNA Markers
DNA Ladders
RNA Markers
GelStar® Nucleic Acid Gel Stain
SeaKem® GTG®Agarose
AccuGENE® TBE and TAE Buffers
SYBR® Green Gel Stains

For Laboratory Use.
SeaKem and GelStar are trademarks of FMC Corporation.
SYBR is a trademark of Molecular Probes. Teflon is atrademark of E.I. duPont de Nemours and Co. All othertrademarks herein are marks of the Lonza Group or itsaffiliates. GelStar is covered by U.S. Patent 5,436,134.
Other US and foreign patents pending.
© 2007 Lonza Rockland, Inc.
All rights reserved.