Modular Micro-spectroscopy Solutions

Features and Benefits• High sensitivity detectors
Ultra-sensitive CCDs, ICCDs and EMCCDs
cameras for maximum photon detection and speed.
Ideal for fast and photon starved chemical mappingor microfluidics setups.
• Highly configurable spectrograph platforms
Extensive range of multi-input & output, motorized
Czerny-Turner spectrographs with seamlesslyinterchangeable and configurable accessories.
• Seamlessly configurable spectrographmicroscope interfaces- fibre optics- direct C-mount- ‘cage’ system- enclosed optical relay
Dedicated feet sets allow easy optical heightmatching between spectrographs and inverted
Nikon, Olympus, Zeiss & Leica microscopes.
• Wide-aperture slit
Kymera and Shamrock input accessory forextended sample image relay AND spectral analysis
through the same optical path.
• μManager software integration
Simultaneous control of Andor cameras &spectrographs and a wide range of microscopes
and accessories through a single software platform.
Dedicated, user-friendly spectrum handlinginterface.
• Software Development Kit (SDK)
Ease of control integration into custom, complex
setups: Matlab, Labview, Visual Basic and C/C++
supported.

Spectroscopy

Adding an analytical dimension
to microscopy images

Highly configurable micro-spectroscopy interfaces
Andor’s dedicated, highly configurable micro-spectroscopy interfaces allow seamlessintegration to microscopy systems. μManager’s user-friendly control of the Kymera
spectrographs and accessories provide a quick and easy setup of complex microspectroscopy acquisition sequences including chemical mapping. Wide-aperture slit options
are also available for extended sample image relay and spectral analysis through the sameoptical path.

Key Applications
 Micro-Raman• Surface-Enhanced Raman Scattering (SERS)• Tip-Enhanced Raman Spectroscopy (TERS)• Semiconductors & Graphene study

 Micro-Luminescence / Photoluminescence / Fluorescence• Carbon nanotubes study• Spectrally-resolved FRET
• Single Molecule spectroscopy• Quantum Dots study• Semiconductors study Plasmonic nanoparticle study Dark-field scattering spectroscopy
 Spectrally-resolved microfluidics / flow cytometry
 Broadband transient spectroscopy

1Modular Micro-spectroscopy Solutions
INVERTED side microscope output – micro-spectroscopy options overview
Key
Please contact
your local Andor
representative

Andor Parts

Microscopeaccessories(inc. filters)

Existing Parts

External light source& microscope
coupling (e.g. laser)

Microscope

Microscope feet

(Inverted side output port)(150 mm optical height)

Fibre optic
microscope adapter

Microscope C-mountflangeCage system
microscope adapter

Fibre optic(SMA, FC, ferrule)

Cage system optomechanical setup(inc. excitationsource)

Enclosed
optical relay

Fibre optic
adapterC-mount
adapter

Motorized/manual
input slits

Cage systemadapter

Wide aperture slit

Filter wheel

Shutter

Solis (S)SDKKymera (193i/328i)
Shamrock (500i/750)

Flat Mirror(grating turret)
Adjustable Feet

Micro-Manager
CCD, EMCCD, ICCD,
InGaAs detectors
Software options
2

Modular Micro-spectroscopy Solutions
UPRIGHT microscope output – micro-spectroscopy options overview
KeyMicroscopeaccessories(inc. filters)

External light source& microscope
coupling (e.g. laser)

Fibre optic
microscope adapter

Please contact
your local Andor
representative

Andor Parts

Existing Parts

Microscope

Microscope feet

(Upright output port)(150 mm optical height)

Microscope C-mountflangeCage system
microscope adapter

Fibre optic(SMA, FC, ferrule)

Cage system optomechanical setup(inc. excitationsource)

Enclosed
optical relay

Fibre optic
adapterC-mount
adapter

Motorized/manual
input slits

Cage system
adapter

Wide aperture slit

Filter wheel

Shutter

Solis (S)SDK

Kymera (193i/328i)
Shamrock (500i/750)

Kymera/Shamrock upright
setup (table, railing etc.)

Micro-Manager
CCD, EMCCD, ICCD,
InGaAs detectors
Software options
3

Modular Micro-spectroscopy Solutions

Spectrograph to microscope – key coupling considerations
Coupling interface
Microscope

SpectrographFibre optics

SMA or FC -to-Cmount adapter

X-Y fibre
optic adapter(ferrule, SMA,or FC)

DirectcouplingStandard ‘male’
C-mount adapter(infinity corrected)

C-mount
adapter plate

Enclosed
optical relay

Standard ‘male’
C-mount adapter(infinity corrected)

C-mountCage
systemStandard ‘male’
C-mount adapter
OR free-space(infinity corrected)

adapter plate

30 mm cage
adapter

Opticalheightmatching
required?

Configurability

No

Limited - Choiceof UV-VIS, VINNIR single ormulticore fibres

Yes-Lightcollection
efficiency

Confocality

Good

Microscope port
connection
SideUprightLow- dictatedby fibre optic
core size

Yes

Yes

Best

Lowest –collection fromentire objective
field of view

Yes

Yes*

Yes

Yes*

Yes

Yes*

Yes

-Good

Lowest –collection fromentire objective
field of view

Yes

Highest - Userdefined, large
range of ‘cage’accessories

Dependant onuser opticalconfiguration

User setupdependant- possibilityof pinhole
integration* Requires user to provide custom spectrograph positioning setup to accommodate this configuration

A Fibre optic coupling – interfacing to microscope upright & side port
Andor Kymera and Shamrock spectrographs offer a range of adjustable X-Y fibre optics input that can accommodate SMA, FC or Ø11 mm ferrule interfaces.
F/# matchers allow maximum collection from NA=0.22 fibre optics assemblies for photon starved applications.

Andor fibre couplers

Kymera/Shamrockspectrograph

Manual Slit
10 µm – 2.5 mm

Motorized Slit
10 µm – 2.5 mm

F/# matcher
For NA=0.22 fibre optics

Wide Aperture Slit
10 µm – 2.5 mm (mot.)12 or 15 mm (man.)X-Y fibre adapterSMA, FC or Ø11mm ferrule

Fibre optics

SMA/FC microscope adapters

SMA-SMA, FC-FC orFerrule to SMA/FC

Off-the-shelf side-port adapters can be found at
www.Thorlabs.com

Part CodeDescription

ACC-MEOPT-8004

1 way fibre, single
50 mm core, UV/VIS,SMA-SMA

ACC-MEOPT-8073

1 way fibre, single
200 mm core, UV/
VIS, SMA-SMA

ACC-MEOPT-8075

1 way fibre, single
100 mm core, UV/VIS,SMA-SMA

SROPT-8002

1 way fibre ferrule,
100 mm core, VIS/NIR

SROPT-8014

1 way fibre ferrule,
100 mm core, UV/VIS

SROPT-8019

1 way fibre ferrule,
200 mm core, VIS/NIR

SROPT-8024

1 way fibre ferrule,
200 mm core, UV/VIS,Microscope

Microscope side port
connection (Thorlabsreferences)

Olympus
IX71/81 &73/83

SM1A51

Leica DMI

SM1A50Nikon Eclipse
Ti-E

SM1A44+SM1FC(FCadapter)
SM1SMA(SMAadapter)

More fibre optics options at:andor.com/spectrographs (Accessories section)

4

Modular Micro-spectroscopy Solutions
B Opto-mechanical coupling - interfacing to microscope side port

1 Microscope & spectrographs feet sets
Free-space optical coupling or ‘hard’ opto-mechanical coupling between Kymera or Shamrock spectrographs and microscope side output
ports require optical axis height matching. Andor offers a range of fixed, rugged microscope feet and adjustable spectrograph feet to allow perfectalignment between micro-spectroscopy system components at an optical height of 150 mm.

Microscope

Microscope fixed feet set

Leica DMI 4000/6000B

TR-LCDM-MNT-150

Leica DMi 8

TR-DMI8-MNT-150

Nikon Eclipse Ti (not Ti2)

TR-NKTI-MNT-150

Nikon Eclipse Ti2

TR-NKTI2-MNT-150

Nikon TE-2000TR-NKTE-MNT-150

Olympus IX71/81 (left port)

TR-OLIX-MNT-150-LP

Olympus IX73/83 (left port)

TR-OL83-MNT-150-LP

Zeiss Axiovert 200

TR-ZSAV-MNT-150

Zeiss Axio Observer

TR-ZAXO-MNT-150

2 Direct C-mount coupling
Kymera/Shamrock ‘female’ C-mount is directly coupled to specificmicroscope C-mount ‘male’ – provides the best light collection
efficiency at the expense of confocality.

Kymera/Shamrock
spectrograph

Manual Slit
10 µm – 2.5 mm

Motorized Slit
10 µm – 2.5 mm

Wide Aperture Slit
10 µm – 2.5 mm (mot.)12 or 15 mm (man.)

C-mount adapter(SR-ASM-0021)

Optical relay(SR-ASZ-0079)

Nominalopticalheight

Optical heightadjustment
range

Adjustablefeet set

Kymera193i

130 mm130 – 136 mm(standard feet)

SR-ASM-0098:
6 mm spacer set*

Kymera328i

143.9 mm

142.6 – 148.6 mm(standard feet)

SR-ASM-0098:
6 mm spacer set

Shamrock500i / 750

146 mm146 – 157 mm(using optional
adjustable feet)

SR-ASM-0082(*) 3 sets are required to accommodate microscope 150 mm optical height

3 Enclosed optical relay
Direct mechanical coupling between a spectrograph and a microscope is
the ideal configuration from an optical efficiency point-of-view. However,in case of physical interference between both instruments footprint, or thepresence of an incubator around the microscope, an optical extender might
be required.
Andor’s optical extender ‘4f’ configuration accommodates collimated andpseudo-collimated beam from the microscope, delivering uncompromising
image relay performance to the Kymera/Shamrock spectrograph input
aperture.

FeatureDetails and Specification

Microscope interface

C-mount (female)

Spectrograph interface

C-mount (male)

Focal plane size

20 x 20 mm

Lens clear apertureØ 25 mmLens material

Fused silica, 400 – 1,000 nm (**)

Magnification

1:1 (horizontal & vertical)

Distortion< 13 µm smile / barrel over focal plane
20 x 20 mm

Overall length

375 mm(**) For alternative wavelength range, please contact your regional sale representative5

Modular Micro-spectroscopy Solutions
4 Cage system adapter
Provides a highly modular, seamlessly upgradable and expandable platform for micro-spectroscopy setups. Andor Kymera/Shamrock spectrographto-microscope cage adapters are compatible with Thorlabs and Edmund Optics large range of 30 mm (*) cage system opto-mechanical accessories.
(*) For different size, please contact your local Andor representative

Wide range of opto-mechanical assemblies available:
Ø 25 mm X-Y-Z-tilt lens or filter holders
SMA/FC fibre connectors
Beamsplitter and prism holders
Pinhole holders
Polarizer holders
Iris and iris mounts
Alignment targets









Andor offers a range of 30 mm cage to microscope adapters:
Microscope

Microscope to cagesystem adapter

Leica DMI 4000/6000B

TR-LCDM-CAGE-ADPLeica DMi 8TR-DMI8-CAGE-ADP

Nikon Eclipse Ti-series

TR-NKTI-CAGE-ADP

Nikon TE-2000

TR-NKTE-CAGE-ADP

Olympus IX71/81 (left port)

TR-OLIX-CAGE-ADP

Olympus IX73/83

TR-OL83-CAGE-ADP

Zeiss Axiovert 200

TR-ZSAV-CAGE-ADP

Zeiss Axio Observer

TR-ZAXO-CAGE-ADP

Generic 30 mm cageadapter

SR-ASM-0065

Example Configuration – ‘4F’ optical relay lensDescription

Part Code#SupplierA

Kymera 193i, 15 mm wide-aperture slit

SR-ASZ-0086

x1

Andor

B

30 mm cage adapter

SR-ASM-0065

x1Andor

ER12ER3
ER1 *ER05 *x4x4x4x4

NT49374-INKx2

Edmund Optics

30 mm cage rods
12” length3” length
1” length0.5” lengthCDØ25 mm lens, VIS-NIR achromaticThorlabs

EXY lens holder, Ø25 mm

CXY1x2Thorlabs

FThread adapter for lens tube

SM1A16x2

Thorlabs

GLens Tubes, Ø30 mm

SM30L10x2Thorlabs

H

Olympus IX81 cage adapter

TR-OLIX-CAGE-ADP

x1

AndorKymera 193i
spectrographfAB

f

2f

CD

EMicroscope, e.g.
Olympus IX81 sideportFGH6

Modular Micro-spectroscopy Solutions
Aperture Slit - Combining sample imaging & spectral analysis through the5 Widesame optical path
‘Infinity-corrected’ microscope ports exhibit quasi-collimated output beam with F/number typically > F/20. When this beam is relayed at the entrance
slit of an ‘imaging-corrected’ spectrograph through direct coupling or low magnification optical arrangement, high fidelity images of the sample underthe microscope objective can be acquired.

Step 1:

Step 2:

Step 3:Sample image visualisation

Sample positioning

Spectral acquisition

input slits are opened Spectrographwidely Grating (or flat mirror) is positionedat the ‘zero’ order, reflecting the undispersed image of the sample
Sample images are acquired by acamera at the output port of thespectrograph The image of the sample area ofinterest is positioned in the middle ofthe spectrograph input slit (by moving
the microscope X-Y stage)

input slits are closed to Spectrographa few 10’s of µm

 Grating is rotated to allow access to

the desired wavelength range
Spectral information from the sample
can be acquired with the detectoroperating in vertical binning of multitrack mode

Above: High fidelity image of at a 25 µm pitch grid pattern on a microscope slide
Microscope: Olympus IX81 with x40 objective (N.A. 0.55) and wide-field illumination.
Detection: Kymera 193i directly coupled to the microscope side port, with a 15 mm wide-aperture slit, a grating at the ‘zero’ order and an iDus 420 CCD camera.

Kymera/Shamrock spectrograph

Grating turret and flat
mirror options

Part CodeDescription

SR-ASZ-0086

Wide-aperture entrance slit, motorised from 10 µm to 2.5 mm,
manual up to 15 mm

SRx-GRT-yyyy-zzz

Grating options
Please refer to individual spectrograph specification sheets atandor.com/spectrographs

SRx-GRT-MRAL+MGF2

Flat mirror option, MgF2-protected aluminium, broadband UV-VIS-IR
Please refer to spectrograph specification sheets for efficiency
curves at andor.com/spectrographs

SRx-GRT-MR-SIL

Flat mirror options, silver-protected, highest efficiency in the VIS-IR
Please refer to spectrograph specification sheets for efficiency
curves at andor.com/spectrographs

Wide Aperture Slit
10 µm – 2.5 mm (mot.)12 or 15 mm (man.)7

Modular Micro-spectroscopy Solutions
C Research-grade spectrographs for Micro-spectroscopy
Highly modular motorized platforms with dual output ports, dual/triple grating turret and wide range of motorized and field upgradable accessories

Kymera 193i – Compact 193 mm imaging
Czerny-Turner spectrograph with Active
Focus technology – ideal for lower spectral
resolution applications.
Shamrock 500i/750 – Ideal for moredemanding, high and higher spectral resolution
applications.

Kymera 328i – Highly configurable platform,
with advanced user controls to always accessthe very best spectral performance for routine
measurements or more demanding opticalsetups.
Learn more about the Andor spectrograph range
and accessories at andor.com/spectrographs

Grating (l/mm)

150300600

1200

1800 (Holo)

2400 (Holo)

Bandpass (nm)•1,•3902

445

215

985646•4

Resolution (nm)•2,•3

1.96

0.96

0.47

0.21

0.12

0.10•4

Bandpass (nm)•1,•3542268

13161

4129•4

Resolution (nm)•2,•3,•5

0.880.62

0.440.31

0.210.15

0.100.07

0.060.05

0.050.04•4

Bandpass (nm)•1,•3357

17786402619•4

Resolution (nm)•2,•30.52

0.26

0.13

0.06

0.04

0.03•4Bandpass (nm)•3,•5242

12059281814•4Resolution (nm)•4,•50.35

0.18

0.09

0.04

0.03

0.02•4Kymera 193i

NEW Kymera 328i

Shamrock 500i

Shamrock 750

Looking for higher optical throughput?

The Andor HoloSpec is a high throughput transmission spectrograph with a robust, compact,
low stray-light design ideal for photon-starved, fibre-coupled setups

D Market leading detectors for Micro-spectroscopy
Best matching requirements for sensitivity at wavelengths of interest, acquisition speed and time-resolution.

iDus 416 ‘low-dark current deepdepletion’ (LDC-DD) CCD – Superior
near-IR detection with up to 95% QE at 800nm.

iDus & Newton ‘Dual AR CCD’(BEX2-DD) – Market leading broadband
detection with >90% QE from 400 to 850 nm.

NewtonEM and iXon Ultra EMCCD– Unmatched sensitivity in the UV-visible
range, superfast kHz acquisition capabilities,single photon sensitive.

iStar ICCD – Nanosecond-gateddetector for characterizing fast transientphenomena.
Learn more about Andor detectors range
at andor.com/scientific-cameras
8Modular Micro-spectroscopy Solutions
E Software solutions
Solis Spectroscopy A 32-bit and fully 64-bit enabled application for Windows (7, 8, 8.1 and 10) offering rich functionality for data acquisition andprocessing, as well as Andor cameras, spectrograph and motorized accessories simultaneous control. AndorBasic provides macro language control of dataacquisition, processing, display and export.

Kymera/Shamrock SDK A software development kit that allows you to control the Andor range of Kymera/Shamrock spectrographs from yourown application. Compatible as 32-bit and 64-bit libraries for Windows (7, 8, 8.1 and 10). Compatible with C/C++, C#, VBNet and LabVIEW and Linux.

4μManager Integrated modular micro-spectroscopy setup

1-3control popular with the Life Science community. All market
leading motorized microscope & accessories seamlessly
controlled and maintained. Integrated sequence builder & macrointerfaces for complex experiments building.
Dedicated spectra display & processing interface plug-in,available through MyAndor.
5 steps to set up your micro-spectroscopy experiment5

1. Set up spectrograph
2. Set up camera
3. Set up microscope
4. Set up experiment e.g. X-Y-Z chemical mapping
5. Display spectral data in real time & save acquisition
series9Modular Micro-spectroscopy Solutions

Order Today
Need more information? At Andor we are committed to finding
the correct solution for you. With a dedicated team of technicaladvisors, we are able to offer you one-to-one guidance andtechnical support on all Andor products. For a full listing of ourandor.com/contact
local sales offices, please see:
Our regional headquarters are:Europe 				Japan
Belfast, Northern Ireland 			Tokyo
Phone +44 (28) 9023 7126			
Phone +81 (3) 6732 8968
Fax +44 (28) 9031 0792			
Fax +81 (3) 6732 8939
North America				China
Concord, MA, USA				Beijing
Phone +1 (860) 290 9211			
Phone +86 (10) 8271 9066
Fax +1 (860) 290 9566			
Fax +86 (10) 8271 9055

Footnotes: Specifications are subject to change without notice
1.
2.
3.
4.
5.

Typical values quoted with 27.6 mm wide CCD, e.g. Newton DU940.
Typical values quoted with 10 μm slit and 13.5 μm pixel CCD, e.g. Newton DU940.
Typical values quoted at 500 nm centre wavelength.
Typical values quoted at 300 nm centre wavelength.
With TrueResTM option.

MicrospecSS 0823 R110