北海道大学
One Health フロンティア卓越大学院プログラム
One Health Allyコース
Student Free Design Activities報告書

Hokkaido University
WISE Program for“One Health Frontier Graduate School of Excellence”
One Health Ally Course
Student Free Design Activities Report frominfluenza virus research. After the talk, theworkshop has officially begun, and the participants
were able to learn the skills such as co-culturing ofMadin-Darby Canine kidney cells together withhuman embryonic kidney 293T cells. Throughout
this training, the participants can understand theimportant role of co-culturing in the reverse geneticsystem as the cell condition and growth rate of two
different cell types can affect the efficiency oftransfection and rescue of the virus. In thisworkshop, I was involved in providing the guidance
and technical support for the participants to ensure
the workshop can be carried out without hindrance.
However, due to abnormalities growth of the cells
occurred during the workshop, I participated in thediscussion with the staff from the government
agencies to optimize the cell conditions in order toensure ideal growth rate of the cell for thetransfection and virus rescue. Because of the time
limitation, we were unable to provide the second
part of the training such as transfection and virus rescue and this training will still be supported by us in the lab
through the online system after returning back to Hokkaido, Japan.
B) Field sampling and observation at LBM, Lang Son, Vietnam- In Vietnam, LBM are the focal point for the AI surveillancebecause of the gathering of poultry such as chickens andducks that were introduced from different regions of Vietnam
or from the neighboring countries. The LBM in Lang Son,Vietnam was selected as the ideal location for the AIsurveillance site because of high risk of the introduction ofHPAIV due to long national border with the neighboring
countries where live birds can be easily transport or smuggled
in and out of Vietnam.
- On 5th of June 2024, we have a brief meeting the governor
officer from the sub-Department of Animal Health (DAH) todiscuss about the current HPAIVs situation and the illegal
trading across the boundaries in Lang Son. In the meeting, we
can understand the situation illegal trading in Lang Son has
been significantly decreased in recent years because of theimplementation of some control measures such as building
fences across the borders or the involvement of multiple cooperation among the government sectors to limit illegal
trading activities across the borders.
- On the next day, 6th of June 2024, we were provided the opportunity to join the field sampling activities in two
LBM, Lang Son and we have an active discussion with the staff from the market management to understand thedisease control level to reduce the spreading of AIV in the market. Even though, difficulties were present incontrolling the spread of HPAIV within the LBM as multiple factors such as from the government side, retailer,or market management were needed to be taken into consideration. However, it was worth noticing that manyefforts have been introduced such as disinfection were carried out at the end of the day and information wereshared to retailers to increase the awareness toward the diseases.

北海道大学One Health フロンティア卓越大学院プログラム
One Health Allyコース
Student Free Design Activities報告書

Hokkaido University
WISE Program for“One Health Frontier Graduate School of Excellence”
One Health Ally Course
Student Free Design Activities Report fromC) Meeting with the staff from DAH, Hanoi, Vietnam- After returning from the surveillance site in Lang Son, we wereprovided the opportunity to have meeting with deputy director ofDAH, which are the head of the epidemiology department together
with other staff within DAH on 7th of June 2024. Information
sharing is important in the controlling transboundary diseases
especially HPAIV and because of the strong collaboration between
DAH and Laboratory of Microbiology, Hokkaido University
which are also the reference laboratory for World Organization for
Animal Health (WOAH). Our member from the lab who oversees
this study shared the results of AI surveillance in Lang Son, Vietnam in 2023 and we also discussed deeply about
the situation of HPAIV in that area and appropriate action needed to reduce the spreading of HPAIV. Besides themembers of DAH also gladly shared the information related to the genetic characteristic of HPAIVs that werecirculating in other part of Vietnam at the moments. The outcome from the meeting further strengthening thecollaboration by enables both parties discuss detail to prioritize in developing better strategy to reduce the spreadof HPAIV within Vietnam as well as in supporting the development of vaccines targeting the latest HPAIV strainsthat can contribute further collaboration.

目的達成状況報告 [Report how you achieved your goal/objectives listed in the application form]
Did you achieve all the goals you initially planned? If not, please describe why you failed to fulfill your objectives.
- The objectives included in the SFDA 4 has been successfully fulfilled for example the workshop for the transfer
for the reverse genetic system organized in NCVD was going smoothly even though some setbacks have been
occurred in the workshop such as the maintenance of the conditions during co-culturing of the cells; the timeline
for the workshop remain unaffected. Throughout this workshop, I was able to understand the importance ofeffective communication and cooperation to ensure the ensure the message successfully delivered to theparticipants in the workshop. For the second part of the activities, which are the field sampling activities in LBM
in Lang Son, Vietnam. This activity has been carried out smoothly, and it gave me different perspective especiallyin the disease control. Apart from focusing on the development of antiviral or vaccine for the treatment or control
the diseases, it required involvement of multiple sectors or agency to control the incident of the disease such as
the effort from DAH to monitor the prevalence and characteristic of HPAIV circulating in the LBM in Vietnam,
the role of the management from the market to increase the awareness of HPAIV among the retailer in the market
or the border control officer to decrease the incident of illegal trading across the borders to minimize theintroduction disease into or out of Vietnam. Lastly, information sharing is important for the disease control, andthe meeting between the members of DAH together with members from Laboratory of Microbiology, Hokkaido
University played an important role to develop a better strategy in disease control.
One Health Approach実践報告 [Report how your activity could link to One Health Approach]Did you have a chance to experience One Health approach (collaboration with people from other academic areas)?
Please describe some of the examples of One Health approach you implemented in your activity. Otherwise, explain
the possibility(ies) how you could link this activity to One Health approach for your future.
- With the concept of the one health approach, international cooperation network played an important role. Thus,to strengthen this cooperation, workshop was organized in NCVD to transfer the reverse genetic technique to thegovernmental agencies in NCVD for the establishment of the vaccine production in the future using this newsystem.
- In this workshop, we can learn the importance of communication skill to facilitate smooth progress during theorganization of the workshop and how to deal with problem if unexpected incident has occurred through effective
communication with other parties. Apart from that, by participating in the field sampling in LBM, Lang Son,Vietnam, it allowed us to understand how the governmental agencies conduct surveillance activities and thestrategies that have implemented to reduce the transmission of avian influenza virus (AIV) in LBM by corporation
with multiple sectors from governor agencies but also the management of border control to ensure the welfare
toward the public health. As Laboratory of Microbiology, Hokkaido University which are also one of the WOAH

北海道大学One Health フロンティア卓越大学院プログラム
One Health Allyコース
Student Free Design Activities報告書Hokkaido University
WISE Program for“One Health Frontier Graduate School of Excellence”
One Health Ally Course
Student Free Design Activities Report fromReference Laboratory for AIV, we joined the active discussion with DAH and NCVD for information sharing
related to AIV situation and the control measure of HPAIV in Vietnam.
- Lastly, this SFDA 4 present a rare opportunity for me (Malaysian) because in Malaysia there were still no reportedof HPAIV outbreak in the poultry, whereby sporadically detection of HPAIV cases were reported in theneighboring countries. Through the one health concept, it allowed me to understand how the governmental bodies
worked to control the HPAIV outbreak in poultry and preparedness for the future potential HPAIV outbreak inMalaysia.
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