Reagents to Support
HuCAL® Assay Development
HuCAL® Fab and Ig antibodies are suitable for use in a diverse range of immunoassays. Typicalapplications include ELISA, flow cytometry, immunofluorescence, immunohistochemistry, andWestern blotting, as well as various functional assays.
This guide provides a compilation of reagents that have been tested and qualified in-house for use
with HuCAL® antibodies.

Contents:
I.
II.
III.
IV.
V.
VI.

Anti-Human Secondary Antibodies
HuCAL® Positive Control Antibodies
HuCAL® Negative Control Antibodies
Anti-Tag Secondary Antibodies
Buffers and Substrates
Antibody Labelling – LYNX Rapid Conjugation Kits®

Suggested protocols, examples, and procedures, as well as a detailed discussion of ourrecombinant antibody technology can be found in our HuCAL® Antibodies Technical Manual (2ndEdition, 2010) or by visiting www.abdserotec.com/HuCAL.

I.

Anti-Human Secondary Antibodies

HuCAL® antibodies in Fab format can be detected by the goat anti-human Fab antibodies shown
below. HuCAL® antibodies in Ig format can be detected in addition by secondary antibodies specificfor the corresponding antibody Fc domain.
Host and Specificity

Product CodeGoat anti-human F(ab')2Goat anti-human F(ab')2, HRP-conjugatedGoat anti-human F(ab')2, Alkaline phosphatase-conjugatedGoat anti-human F(ab')2, Biotin-conjugatedGoat anti-human F(ab')2, FITC-conjugatedGoat anti-human F(ab')2, TRITC-conjugatedGoat anti-human (H + L), F(ab’)2 fragment, R-Phycoerythrin-conjugatedGoat anti-human kappa light chain
Goat anti-human kappa light chain, HRP-conjugatedGoat anti-human kappa light chain, FITC-conjugatedGoat anti-human kappa light chain, F(ab')2 fragment
Goat anti-human kappa light chain, HRP-conjugated F(ab')2 fragment
Goat anti-human kappa light chain, RPE-conjugated F(ab')2 fragment
Goat anti-human lambda light chain
Goat anti-human lambda light chain, HRP-conjugatedGoat anti-human lambda light chain, FITC-conjugatedMouse anti-human IgG, Fc specific
Mouse anti-human IgG, Fc specific, HRP conjugatedMouse anti-human IgG, Fc specific, FITC conjugatedSTAR126
STAR126P
STAR126A
STAR126B
STAR126F
STAR126TRC
STAR97PE
STAR127
STAR127P
STAR127F
STAR128
STAR128P
STAR128PE
STAR129
STAR129P
STAR129F
MCA647G
MCA647P
MCA647F______________________________________________________________________
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II.

HuCAL® Positive Control Antibodies

Specificity

HCA086
HCA145
HCA005
HCA147
HCA023
HCA032
HCA006
HCA053
HCA092
HCA004
HCA002
HCA043

Format (1)Fab-dHLX-MH
Fab-dHLX-FSx2
Fab-dHLX-MH
Fab-dHLX-MSx2
Fab-dHLX-MH
Fab-dHLX-MH
Fab-dHLX-MH
h/m IgG1
Fab-dHLX-MH
Fab-S
Fab-FS
Fab-dHLX-MH

Applications (2)anti-human CD81
anti-human CD127
anti-human Cyclophilin A
anti-human Actin betaanti-human Desmin
anti-human Vimentin
anti-Ki-67
anti-Ki-67
anti-human ADAP
anti-human CD292
anti-FITC
anti-IFN (capture Ab)

Product Code (Clone)anti-IFN (detection Ab)

HCA044Fab-A-FH

E

FF
WB
WB
P, C
P, C
P, C, IFP, CIPFN
EE(1) For format descriptions see our document ‘Choosing the Best HuCAL® Antibody Format’(2) Applications: C = Immunohistology – frozen, E = ELISA, F= Flow cytometry, IP = Immunoprecipitation, P = Immunohistology - paraffin, WB = Westernblotting, IF = Immunofluorescence, FN = Functional AssayIII.

HuCAL® Negative Control Antibodies

Product CodeFormat (1)Applications (2)HCA098HCA045
HCA124
HCA125
HCA100
HCA101
HCA046
HCA052
HCA097
HCA102
HCA104
HCA048HCA047
HCA096
HCA123
HCA106
HCA107
HCA099
HCA139HCA049
HCA108
HCA050
HCA110

Fab-V5H
Fab-FH
Fab-H
Fab-V5Sx2
Fab-MSx2
Fab-FSx2
Fab-FS
Fab-dHLX-MH
Fab-dHLX-FH
Fab-dHLX-MSx2
Fab-dHLX-FSx2
Fab-dHLX-MS
Fab-dHLX-FS
Fab-A-FH
Fab-A-V5Sx2
Fab-A-MSx2
Fab-A-FSx2
Fab-p53-H

E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F
E, WB, P, C, F

Fab-V5H

hIgG1/lambda
hIgG2/lambda
hIgG4/lambda
h/m IgG1 Fc*(1) For format descriptions see our document ‘Choosing the Best HuCAL® Antibody Format’(2) Applications: C = Immunohistology – frozen, E = ELISA, F= Flow cytometry, IP = Immunoprecipitation, P = Immunohistology - paraffin, WB = Westernblotting, IF = Immunofluorescence, FN = Functional Assay* Chimeric Ab consisting of murine Fc and human Fab domains______________________________________________________________________
www.abdserotec.com/HuCAL

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IV.

Anti-Tag Secondary Antibodies

Host and Specificity®

Mouse anti-Strep-tag Immo®
Mouse anti-Strep-tag Classic®
Mouse anti-Strep-tag Classic, HRP-conjugatedMouse anti-His-6-tag
Mouse anti-His-6-tag, HRP-conjugatedMouse anti-His-6-tag, Biotin-conjugatedMouse anti-His-6-tag, FITC-conjugated®
Mouse anti-His-6-tag, Alexa Fluor 488-conjugated®
Mouse anti-His-6-tag, Alexa Fluor 647-conjugatedMouse anti-V5-tag
Mouse anti-V5-tag, HRP
Mouse anti-V5-tag, AP-conjugatedMouse anti-V5-tag, Biotin
Mouse anti-V5-tag, FITC®
Mouse anti-V5-tag, Alexa Fluor 488®
Mouse anti-V5-tag, Alexa Fluor 647
Mouse anti-c-myc-tag (clone 9E10)
Mouse anti-c-myc-tag (clone 9E10), HRP-conjugatedMouse anti-c-myc-tag, Alkaline phosphatase-conjugatedMouse anti-c-myc-tag, Biotin-conjugatedMouse anti-c-myc-tag, FITC-conjugatedMouse anti-c-myc-tag, RPE-conjugated®
Mouse anti-c-myc-tag, Alexa Fluor 488-conjugated®
Mouse anti-c-myc-tag, Alexa Fluor 647-conjugated®
Rat anti-FLAG -tag®
Rat anti-FLAG -tag, HRP-conjugated®
Rabbit anti-FLAG -tag®
Rabbit anti-FLAG -tag, HRP-conjugatedRabbit anti-bacterial alkaline phosphatase, HRP-conjugated

Product CodeTag Detected

MCA2488
MCA2489
MCA2489P
MCA1396
MCA1396P
MCA1396B
MCA1396F
MCA1396A488
MCA1396A647
MCA1360GA
MCA1360P
MCA1360A
MCA1360B
MCA1360F
MCA1360A488
MCA1360A647
MCA2200GA
MCA2200P
MCA2200A
MCA2200B
MCA2200F
MCA2200PE
MCA2200A488
MCA2200A647
MCA4764
MCA4764P
AHP1074
AHP1074P
AHP1108P

S, Sx, Sx2
S, Sx, Sx2
S, Sx, Sx2
H
H
H
H
H
H
V5
V5
V5
V5
V5
V5V5
M
M
M
M
M
M
M
M
F
F
F
F
A

Further options and formats for anti-tag secondary antibodies are available, please inquire.

V.

Buffers and Substrates

Reagent
Antibody diluent
DAB substrate
DAB substrate buffer
HISPEC Buffer
Histar Detection SystemPeroxide Blocking Reagent
Streptavidin-AP
Streptavidin-HRP
Streptavidin-RPE
TMB Plus

Product Code
BUF014BUF021ABUF022BUF049
STAR3000
BUF017A
STAR6B
STAR5B
STAR4B
BUF042A______________________________________________________________________
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VI.

Antibody Labelling – LYNX Rapid Conjugation Kits®
®

HuCAL primary antibodies can be directly labelled with biotin, fluorescent dyes, and enzymes via chemical
coupling in the same manner as conventional antibodies. Our LYNX kits offer a fast and simple conjugation
procedure which requires no purification steps and provides assay sensitivities equal to those achieved withtypical secondary antibodies.

Tandem Dyes

Single Dyes

Enzymes

The table below contains the full range of LYNX kits.
LabelReaction Size

Product CodeBiotin

3 x 200 µg
2 mg

LNK041B
LNK042BAP

100 µg3 x 100 µg
1 mg

LNK011AP
LNK012AP
LNK013AP

HRP

400 µg3 x 400 µg
4 mg
5 x 4 mg
20 mg

LNK001P
LNK002P
LNK003P
LNK004P
LNK005P

Glucose oxidase (GOx)

3 x 100 µg
1 mg

LNK051GOX
LNK052GOX

Allophycocyanin (APC)

150 µg3 x 150 µg
1.5 mg

LNK031APC
LNK032APC
LNK033APC

Cy53 x 200 µg
2 mg

LNK101CY5
LNK102CY5Fluorescein

3 x 200 µg
2 mg

LNK061F
LNK062F

PerCP

100 µg3 x 100 µg
1 mg

LNK071PERCPLNK072PERCPLNK073PERCP

RPE

60 µg
3 x 60 µg
600 µg

LNK021RPE
LNK022RPE
LNK023RPE

APC-Cy5.5150 µg
3 x 150 µg
1.5 mg

LNK121APCCY5.5
LNK122APCCY5.5LNK123APCCY5.5APC-Cy7150 µg
3 x 150 µg
1.5 mg

LNK131APCCY7LNK132APCCY7LNK133APCCY7PerCP-Cy5.5100 µg
3 x 100 µg
1 mg

LNK141PERCPCY5.5
LNK142PERCPCY5.5
LNK143PERCPCY5.5RPE-Cy560 µg
3 x 60 µg
600 µg

LNK081C
LNK082C
LNK083C

RPE-Cy5.560 µg
3 x 60 µg
600 µg

LNK091PECY5.5LNK092PECY5.5
LNK093PECY5.5RPE-Cy760 µg
3 x 60 µg
600 µg

LNK111PECY7LNK112PECY7LNK113PECY7Further options and formats for LYNX kits are available, please inquire.

For more information on HuCAL® antibodies, LYNX Rapid Conjugation Kits® or related
antibody reagents, search online at www.abdserotec.com.
HuCAL® is a registered trademark of MorphoSys AG. LYNX Rapid Conjugation Kits® is a registered trademark of MorphoSys UK Ltd.
Alexa Fluor® is a registered trademark of Molecular Probes Inc, OR, USA. DyLight® is a registered trademark of Thermo Fisher Scientificand its subsidiaries. FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Strep-Tag® is a
registered trademark of IBA, GMBH.

______________________________________________________________________
www.abdserotec.com/HuCAL

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