Product datasheet

Anti-Collagen IV antibody ab6586
120 Abreviews 920 References 画像数 7
製品の概要
製品名

Anti-Collagen IV antibody

製品の詳細

Rabbit polyclonal to Collagen IV

由来種Rabbit

特異性Some class-specific anti-collagens may be specific for three-dimensional epitopes which may
result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded
tissues. This antibody reacts with most mammalian Type IV collagens and has negligible crossreactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagenantibodies with other human serum proteins or non-collagen extracellular matrix proteins isnegligible.

アプリケーション

適用あり : ELISA, IHC-Fr, WB, IHC-P, IP, ICC/IF, IHC-FrFl, IHC-FoFr

種交差性

交差種 : Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Zebrafish, African green monkey,

Chinese hamster, Syrian hamster
交差が予測される動物種 : Mammals
免疫原This product was produced with the following immunogens:
Full length native protein (purified) corresponding to Human Collagen IV. Collagen Type IV fromhuman and bovine placenta. The immunogen maintains the native conformation of the protein.
Database link: P02462
Full length native protein (purified) corresponding to Bovine Collagen IV.
Database link: Q7SIB2

ポジティブ･コントロール

IHC-P: Human kidney, liver, lung, renal oncocytoma and skeletal muscle tissues.

特記事項

There are other recombinant monoclonal options, such as Recombinant Anti-Collagen IV antibody
ab214417.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit
Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use withyour experiment.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years.
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies
and knockout edited cell lines for gold-standard validation. Please check that this product meets
your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/orcontact our Support team ahead of purchase. Recommended alternatives for this product can befound below, along with publications, customer reviews and Q&As
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製品の特性
製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C longterm. Avoid freeze / thaw cycle.

バッファー

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

精製度

Immunogen affinity purified

特記事項（精製）

Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized
antigens followed by extensive cross-adsorption against other collagens, human serum proteinsand non-collagen extracellular matrix proteins to remove any unwanted specificities.

一次抗体 備考This antibody is well suited to detect extracellular matrix proteins in normal as well as diseasestate tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions canbe distinguished from benign by examining the breakdown of basement membranes and loss of
3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases todegrade barriers created by the extracellular matrix which then allows metastasis to occur.
Collagenases, stomelysins and gelatinases can collectively degrade all of the various
components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement
membrane glycoproteins.

ポリ /モノ

ポリクローナルアイソタイプ

IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6586の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション

特記事項

Abreviews

ELISA

Use at an assay dependent concentration.

IHC-Fr(21)

Use at an assay dependent concentration.

WB

(26)

Use at an assay dependent concentration. Predicted molecularweight: 161 kDa.
This product is not recommended for use under denaturingconditions in WB, IP, and ELISA. We would suggest testing it
under native conditions.

IHC-P

(36)

1/15 - 1/400. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.

IP

(3)

Use at an assay dependent concentration.

ICC/IF(23)

Use at an assay dependent concentration. PubMed: 19933193

IF

Use at an assay dependent concentration.
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アプリケーション

特記事項

Abreviews

IHC-FrFl(1)

Use at an assay dependent concentration.

IHC-FoFr(8)

Use at an assay dependent concentration.

ターゲット情報機能

Type IV collagen is the major structural component of glomerular basement membranes (GBM),
forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The
C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cellproliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alphaand ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

組織特異性

Highly expressed in placenta.

関連疾患Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH)[MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and alarger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms andmuscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria andbilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidneyand skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly
is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1
is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion orbirth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a
primary defect or arrest in the development of the cerebral ventricles.

配列類似性

Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

ドメイン

Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus,
frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may
cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

翻訳後修飾

Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases
and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or allof the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- andintramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all
known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Metresidues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten.

細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane.

画像3

Immunohistochemistry analysis of paraformaldehyde perfusion fixedfrozen pig kidney permeabilized with 0.05% tween 20. Stained withab6586 at 1/200 dilution. Secondary antibody used was AlexaFluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
at 1/300 dilution. The nuclei are stained Blue with Hoechst. The Red
counter stain was created with a methyl green stain and by imaging
with a 555nm excitation filter. Blocking was done with Sea Block for
30 minutes at 22°C.
The sample was incubated with the primary antibody and Sea Blockfor 16 hours at 4°C. Antigen retrieval method was heat mediatedwith Citrate Buffer pH 6.0.
Immunohistochemistry (PFA perfusion fixed frozen
sections) - Anti-Collagen IV antibody (ab6586)
This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embeddedsections) analysis of formaldehyde fixed human brain (cortex)
staining with ab6586 at 1/250 dilution. Secondary antibody used
was HRP Goat Agilent anti-mouse/rabbit EnVision Dual Link.
Blocking was done using 5% serum for 15 minutes at 20°C. The
sample was incubated with the primary antibody for 1 hour at 20°C
with Agilent antibody diluent. Antigen retrieval method was heat
mediated Citrate

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-Collagen IV antibody(ab6586)
This image is courtesy of an anonymous Abreview

4Immunohistochemical analysis formalin-fixed paraffin-embedded
human lung tissue labelling Collagen IV with ab6586 at 1/15 dilution
for 1 hour at 37 °C followed by a ready to use Polymer-HRP,
Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking
Solution for 10 minutes. Substrate: DAB-Chromogen,Rabbit/Mouse. Staining/Results: basement membranes andvessels. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citratebuffer pH 7.8 for 5 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-Collagen IV antibody(ab6586)

Immunohistochemical analysis of formalin-fixed paraffin-embedded
human skeletal muscle cells labelling Collagen IV with ab6586 at
1/15 dilution for 1 hour at 37 °C followed by a ready to use PolymerHRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking
Solution for 10 minutes. Substrate: DAB-Chromogen,Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV
fibers. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citratebuffer pH 7.8 for 5 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-Collagen IV antibody(ab6586)

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Immunohistochemical analysis of formalin-fixed paraffin-embedded
human renal oncocytoma tissue labelling Collagen IV with ab6586
at 1/15 dilution for 1 hour at 37 °C followed by a ready to use
Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: PeroxidaseBlocking Solution for 10 minutes. Substrate: DAB-Chromogen,Rabbit/Mouse. Staining/Results: dense collagen IV positive
membranes surrounding tumor cell nests. Counterstained withhematoxylin for 15 seconds
Heat induced epitope retrieval (HIER) using Tris-EDTA-citratebuffer pH 7.8 for 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-Collagen IV antibody(ab6586)

Paraffin-embedded human kidney tissue stained for Collagen IVusing ab6586 at 1/400 dilution in immunohistochemical analysiswith strong staining observed in glomeruli.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-Collagen IV antibody(ab6586)

Paraffin-embedded human liver tissue stained for Collagen IV using
ab6586 at 1/400 dilution in immunohistochemical analysis, strong
staining was observed in the sinusoids.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-Collagen IV antibody(ab6586)6

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