Product datasheet

Anti-COX IV antibody - Mitochondrial Loading Control ab16056
33 Abreviews 272 References 画像数 11
製品の概要
製品名

Anti-COX IV antibody - Mitochondrial Loading Control

製品の詳細

Rabbit polyclonal to COX IV - Mitochondrial Loading Control

由来種

Rabbitアプリケーション

適用あり : IHC-Fr, WB, ICC/IF, IHC-P

種交差性

交差種 : Mouse, Rat, Human, Xenopus laevis, Potato, African green monkey, Chinese hamster
交差が予測される動物種 : Chimpanzee, Monkey

免疫原Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

ポジティブ･コントロール

ICC/IF: HeLa cells.

特記事項This antibody makes an effective loading control for mitochondria. COX IV is generally expressed
at a consistent high level. However, be aware that many proteins run at the same 16kD size as
COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for
proteins of this size. Some caution is required when using this antibody as a loading control as
COXIV expression can vary under some manipulations. An alternative mitochondrial loading
control is Mouse monoclonal to COX IV antibody [20E8] (ab14744).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years.
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies
and knockout edited cell lines for gold-standard validation. Please check that this product meets
your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or
contact our Support team ahead of purchase. Recommended alternatives for this product can befound below, along with publications, customer reviews and Q&As

製品の特性
製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.

バッファー

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

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Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising
agent. If you would like information about the formulation of a specific lot, please contact ourscientific support team who will be happy to help.
精製度

Immunogen affinity purified

一次抗体 備考

This antibody makes an effective loading control for mitochondria. COX IV is generally expressed
at a consistent high level. However, be aware that many proteins run at the same 16kD size as
COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for
proteins of this size. Some caution is required when using this antibody as a loading control as
COXIV expression can vary under some manipulations. An alternative mitochondrial loading
control is Mouse monoclonal to COX IV antibody [20E8] (ab14744).

ポリ /モノ

ポリクローナルアイソタイプIgG

アプリケーション

The Abpromise guaranteeAbpromise保証は、 次のテスト済みアプリケーションにおけるab16056の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション

特記事項

Abreviews

IHC-Fr(2)

Use at an assay dependent concentration.

WB

(15)

Use a concentration of 0.5 µg/ml. Detects a band of
approximately 15 kDa (predicted molecular weight: 17 kDa).

ICC/IF(6)

Use a concentration of 1 µg/ml.

IHC-P

(5)

Use a concentration of 1 µg/ml. Perform heat mediated antigen
retrieval with citrate buffer pH 6 before commencing with IHC
staining protocol.

ターゲット情報機能This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal
oxidase in mitochondrial electron transport.

組織特異性

Ubiquitous.

配列類似性

Belongs to the cytochrome c oxidase IV family.

細胞内局在Mitochondrion inner membrane.

画像2

All lanes : Anti-COX IV antibody - Mitochondrial Loading Control(ab16056) at 1/3000 dilution

All lanes : mouse heart whole cell lysate
Lysates/proteins at 20 µg per lane.
Western blot - Anti-COX IV antibody - Mitochondrial

SecondaryLoading Control (ab16056)

All lanes : Goat Anti-rabbit IgG, HRP-linked Antibody at 1/1000

This image is courtesy of an anonymous Abreview

dilution
Developed using the ECL technique.
Performed under non-reducing conditions.

Predicted band size: 17 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

15% gelImmunohistochemistry analysis (Formalin/PFA-fixed paraffinembedded sections) of paraformaldehyde fixed mouse heart tissue.
Stained with ab16056 at 1/500 dilution.
Secondary antibody used was EnVision+ System- HRP Labelled
Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at21°C. The sample was incubated with the primary antibody and 5%
normal goat serum for 19 hours at 4°C. Antigen retrieval method
was heat mediated Citrate pH 6.0

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-COX IV antibody Mitochondrial Loading Control (ab16056)
This image is courtesy of an anonymous Abreview

3Immunohistochemistry analysis (Formalin/PFA-fixed paraffinembedded sections) of paraformaldehyde fixed human brain tissue.
Stained with ab16056 at 1/150 dilution. Secondary antibody used
was EnVision+ System- HRP Labelled Polymer anti rabbit.
Blocking was done with 5% serum for 1 hour at 21°C. The sample
was incubated with the primary antibody and 5% normal goat serumfor 19 hours at 4°C. Antigen retrieval method was heat mediatedCitrate pH 6.0

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-COX IV antibody Mitochondrial Loading Control (ab16056)
This image is courtesy of an anonymous Abreview

All lanes : Anti-COX IV antibody - Mitochondrial Loading Control(ab16056) at 1 µg/ml

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue
Lysates/proteins at 10 µg per lane.

SecondaryAll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed
Western blot - Anti-COX IV antibody - Mitochondrial(HRP) at 1/50000 dilution

Loading Control (ab16056)

Predicted band size: 17 kDa
Observed band size: 17 kDa

Blocking buffer: 2% BSA
Gel type: MES
Exposure Time: 1 minute4

ab16056 staining COX IV in HeLa cells. The cells were fixed with
4% paraformaldehyde (10 min), permeabilized with 0.1% PBSTriton X-100 for 5 minutes and then blocked with 1% BSA/10%
normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The
cells were then incubated overnight at 4°C with ab16056 at 1µg/ml
and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading
Control. Cells were then incubated with ab150081, Goat polyclonal
Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat
polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®
594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red).
Immunocytochemistry/ Immunofluorescence - Anti-

Nuclear DNA was labelled with DAPI (shown in blue).

COX IV antibody - Mitochondrial Loading Control

Also suitable in cells fixed with 100% methanol (5 min).

(ab16056)

Image was acquired with a high-content analyser (Operetta CLS,
Perkin Elmer) and a maximum intensity projection of confocal
sections is shown.

All lanes : Anti-COX IV antibody - Mitochondrial Loading Control(ab16056) at 1 µg/ml

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.

SecondaryAll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed
Western blot - Anti-COX IV antibody - Mitochondrial(HRP) at 1/50000 dilution

Loading Control (ab16056)

Predicted band size: 17 kDa
Observed band size: 17 kDa

Blocking buffer: 2% BSA
5

Gel type: MES
Exposure Time: 1 minuteAll lanes : Anti-COX IV antibody - Mitochondrial Loading Control(ab16056) at 1 µg/ml

Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein(ab29330)
Lysates/proteins at 10 µg per lane.
Western blot - Anti-COX IV antibody - Mitochondrial
Loading Control (ab16056)

SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed(ab7090) at 1/3000 dilution
Performed under reducing conditions.

Predicted band size: 17 kDa

ab16056 staining COX IV in Mouse heart tissue sections byImmunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffinembedded sections). Tissue was fixed with paraformaldehyde andblocked with 5% serum for 1 hour at 21°C; antigen retrieval was byheat mediation in a citrate buffer. Samples were incubated withprimary antibody (1/600 ) for 12 hours at 4°C. A Cy5® donkey antirabbit secondary antibody was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-COX IV antibody Mitochondrial Loading Control (ab16056)
This image is courtesy of an anonymous Abreview

6All lanes : Anti-COX IV antibody - Mitochondrial Loading Control(ab16056) at 0.38 µg/ml

Lane 1 : HeLa whole cell lysate
Lane 2 : Human skeletal muscle cell lysate
Lane 3 : HeLa whole cell lysate with Human COX IV peptide(ab16381) at 1 µg/ml

Lane 4 : Human skeletal muscle cell lysate with Human COX IVpeptide (ab16381) at 1 µg/ml
Western blot - Anti-COX IV antibody - Mitochondrial
Loading Control (ab16056)

Lysates/proteins at 20 µg per lane.

SecondaryAll lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 15 kDa

IHC image of COXIV staining in human liver FFPE section,
performed on a BondTM system using the standard protocol F. Thesection was pre-treated using heat mediated antigen retrieval withsodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins.
The section was then incubated with ab16056, 1µg/ml, for 8 mins atroom temperature and detected using an HRP conjugated compact
polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-COX IV antibody Mitochondrial Loading Control (ab16056)

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ab16056 staining COX IV in breast tumour tissue sections byImmunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffinembedded sections). Tissue was fixed with 10% buffered formalin
and blocked with 5% serum for 60 minutes at 21°C; antigen
retrieval was by heat mediation in a 10mM sodium citrate buffer
pH6. Samples were incubated with primary antibody (1/300 inblocking buffer) for 12 hours at 4°C. An Alexa Fluor® 647conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as
the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-COX IV antibody Mitochondrial Loading Control (ab16056)
This image is courtesy of an anonymous Abreview.

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