Anti-At 12S (12S Globulin) (At) antibody, rabbit polyclonal
Product code81-123Size

200 µgStorage-20℃

Concentration

2.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenA.thaliana 12S globulinα-subunit purified on SDS-PAGE.

IsotypeRabbit IgG

Reactivity

Arabidopsis thaliana 12S globulin (precursor andα-subunit).
Not tested in other species.

Special notes

N/A

Application

1. Western blotting (1/3,000-1/10,000)
2. Immuno-electron microscopy (1/50)
3. Immunohistochemistry (1/100)
4. ELISA (Assay dependent)

Background

Major 12S seed storage protein CRC (globulin) is synthesized on theendoplasmic reticulum as precursor and then transported to storage vacuoles,
where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl
endopeptidase to produce two mature polypeptides referred to as alpha and beta
subunits that are joined together by a disulfide bond. Phosphorylated in seeds
on some Tyr residues in response to abscisic acid (ABA)
Subcellular location: Vacuole

Data Link

UniProtKB:Q96318 (CRU3_ARATH)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 81-123 Anti-12S (12S Globulin) (At) antibody, rabbit polyclonal

Fig.1

Western Blot of the precursor 12S globulin and 12S-αsubunit of the mature forms in extractof arabidopsis dry seeds.
M: Protein size markers (kDa)
1: SDS-PAGE of wild-type seed.
2: SDS-PAGE of of atvst1-1 mutant (defective in storage protein transport) seed.
3. Western blot of wild-type seed
4. Western blot of atvst1-1 mutant seed
The antibody was used at 1/5,000 dikution.
P12S is the precursor of 12S globulin.
12S-α is the α-subunit of 12S globulin.
Reference: This product has been used in the following publications.
1. Shimda T. et al. Vacuolar processing enzymes are essential for proper processing of seed storage
proteins in Arabidopsis thaliana. J Biol Chem. 2003 Aug 22;278(34):32292-9. PMID:12799370.
WB (Arabidopsis)
2. Shimada T. et al. Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana. Proc
Natl Acad Sci U S A. 2003 Dec 23;100(26). PMID:14657332 WB, Immuno-Electron Microscopy(Arabidopsis)
3. Shimada T. et al. AtVPS29, a putative component of a retromer complex, is required for theefficient sorting of seedstorage proteins. Plant Cell

Physiol. 2006

Sep;47(9):1187-94.

PMID:16926167 WB, Immuno-Electron Microscopy (Arabidopsis)
4. Li L. et al. MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in
Arabidopsis thaliana. Plant Cell. 2006 Dec;18(12):3535-47. PMID: 17194767. WB, Immuno2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/Electron Microscopy (Arabidopsis)
5. Tamura K. et al. Arabidopsis KAM2/GRV2 is required for proper endosome formation andfunctions in vacuolar sorting and determination of the embryo growth axis. Plant Cell. 2007
Jan;19(1):320-32. PMID:17259264 WB, Immuno-Electron Microscopy (Arabidopsis)
6. Fuji K. et al. Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified
efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds. Plant
Cell. 2007 Feb;19(2):597-609. PMID:17293568. WB (Arabidopsis)
7. Takahashi H. et al. MAG4/Atp115 is a golgi-localized tethering factor that mediates efficientanterograde

transportin

Arabidopsis.

Plant

Cell

Physiol. 2010

Oct;51(10):1777-87.PMID:20837504 WB, Immuno-Electron Microscopy (Arabidopsis)
8. Shirakawa M. et al. Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellularregions function redundantly in vacuolar protein sorting and plant development. The Plant
Journal (2010) 64, 924–935. PMID:21143674 WB, Immuno-Electron Microscopy (Arabidopsis)
9. Li L. et al. MAG2 and three MAG2-INTERACTING PROTEINs form an ER-localized complex tofacilitate storage protein transport in Arabidopsis thaliana. Plant J. 2013 Dec;76(5):781-91.
PMID:24118572 WB, Immuno-Electron Microscopy (Arabidopsis)
10. Shirakawa M. et al. CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized
membrane protein required for Golgi morphology and vacuolar protein sorting. Plant Cell
Physiol. 2014 Apr;55(4):764-72. PMID:24363287 WB, IHC (Arabidopsis)
11. Teh OK. et al. BEACH-domain proteins act together in a cascade to mediate vacuolar protein
traffickinganddisease

resistancein

Arabidopsis.

MolPlant. 2015

Mar;8(3):389-98.

PMID:25618824 WB (Arabidopsis)
Related products81-121 Anti-2S3P (2S Albumin precursors, At) antibody, rabbit polyclonal
81-122 Anti-2S3M (2S Albumin mature forms, At) antibody, rabbit polyclonal

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/