Anti-HHV7 gH antibody, mouse monoclonal (clone 2)
Product code65-210Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenHis6-tagged recombinant gH of HHV-7 encoding 333-636 amino acids expressed inE. coli.

Isotype

Mouse IgG1κ

Reactivity

Reacts with gH of HHV7.

Special notes

N/AApplication

1. Western blotting (1/500~1/1,000 dilution)
2. Immunoprecipitation (assay dependent)

Background3.

Imunofluorescence staining and Immunocytochemistry (1/100~1/3,200 dilution)

4.

ELISA (assay dependent)

Humanherpesvirus

7 (HHV-7)

the Herpesviridae family

thatisinfects

oneofninehumans.

known

HHV-7

ismembers
aofmemberof Betaherpesviridae, a subfamily of the Herpesviridae that also includes HHV6 and cytomegalovirus (HHV-5 or HCMV). HHV-7 often acts together with HHV-6,and the viruses together are sometimes referred to by their genus, Roseolovirus.
HHV-7 was first isolated in 1990 from CD4+ T cells taken from peripheral bloodlymphocytes.

Both HHV-6B and HHV-7, as well as other viruses, can cause a skincondition in infants known as exanthema subitum, although HHV-7 causes thedisease less frequently than HHV-6B. HHV-7 infection also leads to or is associatedwith a number of other symptoms, including acute febrile respiratory disease, fever,rash, vomiting, diarrhea, low lymphocyte counts, and febrile seizures, though mostoften no symptoms present at all.
Herpesviruses encode several glycoproteins that are targeted to the virion
envelope. They play critical roles in viral infection, including attachment,penetration, cell-to-cell spread and the maturation of nascent viral particles. In
human herpesviruses, envelope glycoprotein H (gH) associates with glycoprotein L(gL) to form a gH–gL complex, which is a key participant in fusion events critical toherpesvirus infection.

Data Link

N/A

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-210 Anti-HHV7 gH antibody, mouse monoclonal (clone 2)

Fig.1. Immunoprecipitation of HHV7 gH in the lysate of HHV7 infected-SupT-1 cells.
1st lane: HHV7 infected SupT-1 cell lysate
2nd lane: Mock Sup1 cell lysate
3rd lane: Virion
The antibody conjugated with agarose was used for immunoprecipitation and for western blotting,the antibody was used at 1/1,000 dilution.
gH-90 may be the precursor and gH-80, the mature form of gH glycoprotein.

Fig.2. Immunofluorescence staining of gH protein in 293T cells transfected with gH expessing
plasmid.
The plasmid pCAGGS/gH was transfected and two days later, the cells were harvested and fixed withmethanol and stained with indirect immunofluores using this antibody and fluorescein-conjugatedgoat antibodies against mouse IgG.
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Fig.3. Immunofluorescence staining of gH protein in SupT1 cells infected with HHV-7HHV-7 infected SupT1 cells (at 4 days post infection; dell-to-cell spreading)
Aceton/Methanol (70%/30%) fixation and permeabilization.
Anti-HHV-7 gH antibody at 1/100 dilution
Alexa Fluor 488 conjugated donkey anti-mouse IgG at 1/300
Hoechst33342 at 1/1,000 dilution
References: This antibody has been described and used in the following publication...
Sadaoka T, Yamanishi K, Mori Y. Human herpesvirus 7 U47 gene products are glycoproteins
expressed in virions and associate with glycoprotein H. J Gen Virol. 2006 Mar;87(Pt 3):501-8. PMID:16476971. WB, IP, IF

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