TECHNICAL SPECIFICATIONS

VisionSort Platform
VisionSort brings together fundamental advances in optics, microfluidics, andartificial intelligence (AI) to deliver morphological profiling and label- freecell sorting on top of the capabilities you have come to expect from traditional
fluorescence-only cytometers.

GHOST CYTOMETRY

Powered by scientifically established Ghost Cytometry technology and three fluorescence lasers, VisionSort enables
researchers to perform real-time, AI-driven, single cell sorting with or without labels. Flexible, easy-to-use, and user- controlled
AI algorithms embedded directly in the instrument enable unbiased morphometric characterization and isolation of rare andunique cell populations.

OPTICSLasers

PERFORMANCE
405 nm - 365 mW nominal output power(Structured Illumination)

Fluorescencesensitivity

FITC: <1000 molecules of equivalent soluble
fluorochrome (MESF-FITC)

637 nm - 160 mW nominal output power

Fluorescence
resolution< 5% (HPCV)

Forward scatter (FSC)

Purity and yield

With an average sample cell flow rate of
1,000 events per second, a one-way sortachieved purity of >98% and yield >80%of Poisson’s expected yield. Higher flow
rates up to 3,000 events per second can beachieved without affecting purity; however,yield decreases based on Poisson statistics.

Cell flow rate

Up to 3,000 cells/s (1.1x107 cells/h)

488 nm - 150 mW nominal output power

Scatter detectors

Backward scatter (BSC)
Fluorescencedetectors

5 channels
Blue: 440/40 nm (405 nm excitation)
Green: 525/50 nm (488 nm excitation)
Yellow: 600/37 nm (488 nm excitation)
Red: 680/42 nm (637 nm excitation)
Infrared: 785/42 nm (637 nm excitation)Ghost Motion
Image (GMI) signal
detectors

Forward scattered GMI signal (fsGMI)
Backward scattered GMI signal (bsGMI)
Brightfield GMI signal (bfGMI)
Diffractive GMI signal (dGMI)
Fluorescence GMI signal (405 nm excitation,
440 nm emission)

Objective lens

1.2 mL/h (Processing Rate)
Detection rate

Up to 3,000 events per second

Viability>99% for lymphocytes

Maximum operatingpressure(Pressure appliedto cells)

Up to 150 kPa (21 psi)

0.63 µm (NA: 0.8)

FLUIDICSSORTING CARTRIDGE

Sample input

5 mL round bottom tube and 15 mL conicaltube

Sample collection

15 mL and 50 mL conical tubes

Fluidic reservoirs

10 L sheath
10 L waste
5 L deionized (DI) water

Minimum sample
volume100 µL (when using 5 mL round bottom tube)

Dead volume<50 µL (when using 5 mL round bottom tube)

Temperature
controlTemperature of inlet and outlet is software-adjustable: Cool to room temperature(~12 - 23 °C, 50 - 73 °F )VisionSort Platform Technical Specifications

Material

PDMS (Dimethylpolysiloxane), GlassSize

136 x 88 x 7.5 mm (5.4 x 3.5 x 0.3 in)Channel dimension

32.5 x 50 µm

Target particle size

4-40 µm

Maximum loadingcell number

1 x 107 cells/mL

Sorting mode

One-way (Positive / Other), Two-way viable
cell collection

4

SYSTEM & SOFTWAREINSTALLATION DETAILSWorkstation CPUIntel Core i9-10900TE

Dimensions(W x D X H)

Operating system

Windows®10 IoT Enterprise LTSC

Signal processing

14-bit analog-to-digital conversion and signal
processing

154 x 76 x 151 cm(60.6 x 29.9 x 59.5 in)
System Cabinet
92 x 76 x 151 cm (1.05 m3);
(36.2 x 29.9 x 59.5 in)Control Cabinet
61 x 75 x 98 cm (0.45 m3)(24.0 x 29.5 x 38.6 in)

USB ports

2 x USB 3.0

Ethernet

1 PortMonitors

2 x 27’’ LCDs, 1,920 x 1,080-pixel resolution

System Cabinet
328 kg (723 lbs.)

Memory64 GB (DDR4)

Control Cabinet
103 kg (227 lbs.)

Storage

240 GB SATA SSD; 8 TB SATA SSD

Software

VisionSort Control Software

Data types*.gcs (proprietary file type)

WeightScatter Height, Width, and Area andconventional fluorescence can be exported
to Flow Cytometry Standard (FCS) 3.1 fileformat (*.fcs)

THINKCYTE INC.
UNITED STATES
1100 Island Drive, STE 203
Redwood City, CA 94065

431 kg (950 lbs.)

Powerrequirements

100-120 VAC, 15 A Max

Pressure supply
requirements

700-820 kPa (100-120 psi)

Operatingtemperature

17-23 °C

220-240 VAC, 10 A Max

Operating humidity 5-80% relative humidity, non-condensing

CONTACT@THINKCYTE.COMJAPAN
7-3-1 Hongo,Bunkyo, Tokyo

WWW.THINKCYTE.COM

FOR RESEARCH USE ONLY. © Copyright 2024. All rights reserved. ThinkCyte,
VisionSort and Ghost Cytometry are trademarks of ThinkCyte K.K. All othertrademarks are the property of their respective owners. TS-013-01.23.1-USUnauthorized posting of this material in the public domain is prohibited.