RESEARCH PRODUCTS

Cambridge Isotope Laboratories, Inc.
isotope.com

SILAM Feeds
Stable Isotope Labelingin Mammals

Customfeeds can beprepared uponrequest.

The study of animal models of disease provides useful insight into human disease. The SILAM technique allows the global, relative
quantitative analysis of mammalian disease models through case-control analyses. In traditional SILAM, rodents are fed either an
isotope-rich or isotope-deficient diet for a period of time (tissue-specific due to turnover rates) before LC-MS analysis of theharvested tissue(s) of interest.
Cambridge Isotope Laboratories, Inc. (CIL) is pleased to offer labeled/unlabeled feeds (see tables below) for metabolic
incorporation of stable isotope-enriched or natural amino acids into mice (or rats) for use in SILAM-based MS studies.

Mouse Express® L-Lysine

Spirulina and Mouse Express®

Catalog No.
MF-LYS-C
MLK-LYS-C
MF-LYS-C-IR

Catalog No.
CLM-8400
NLM-8401
ULM-8453
MF-Spirulina-N
MF-Spirulina-U
MLK-Spirulina-N
MF-Spirulina-N-IR

MLK-LYS-C-IR

Description
Mouse Express L-Lysine (13C6, 99%) Mouse Feed*
Mouse Express L-Lysine (13C6, 99%) Mouse Feed Kit
Mouse Express L-Lysine (13C6, 99%)
Irradiated Mouse Feed*
Mouse Express L-Lysine (13C6, 99%)
Irradiated Mouse Feed Kit*Unlabeled Mouse Express mouse feed (MF-UNLABELED) is also available.
Note: These kits contain 1 kg of 13C-labeled (99%) and unlabeled L-lysine feed.

Mouse Express® L-Leucine
Catalog No.
MF-LEU-D3
MLK-LEU-D3
MF-LEU-D3-IR
MLK-LEU-D3-IR

Description
Mouse Express L-Leucine (5,5,5-D3, 99%)
Mouse Feed*
Mouse Express L-Leucine (5,5,5-D3, 99%)
Mouse Feed Kit
Mouse Express L-Leucine (5,5,5-D3, 99%)
Irradiated Mouse Feed*
Mouse Express L-Leucine (5,5,5-D3, 99%)
Irradiated Mouse Feed Kit*Unlabeled Mouse Express mouse feed (MF-UNLABELED) is also available.
Note: These kits contain 1 kg of D3-labeled (99%) and unlabeled L-leucine feed.

Description
Spirulina Whole Cells (U-13C, 97%)
Spirulina Whole Cells (U-15N, 98%)
Spirulina Whole Cells (unlabeled)
Mouse Express Spirulina (15N, 98%) Mouse Feed
Mouse Express Spirulina (unlabeled) Mouse Feed
Mouse Express Spirulina (15N, 98%) Mouse Feed Kit
Mouse Express Spirulina (15N, 98%)
Irradiated Mouse Feed
MF-Spirulina-U-IR
Mouse Express Spirulina (unlabeled)
Irradiated Mouse Feed
MLK-Spirulina-N-IR Mouse Express Spirulina (15N, 98%)
Irradiated Mouse Feed Kit
Note: The kits contain 1 kg of 15N-labeled (98%) and unlabeled spirulina feed.

Mouse Express® L-Lysine NeuCode™
Catalog No.
Description
MF-LYS-NEU2-1WK Mouse Express L-Lysine 2-plex NeuCode Mouse Feed

Mouse Express is a registered trademark of Cambridge Isotope Laboratories, Inc.
NeuCode™ is a trademark of WARF.

Please inquire if alternative formulations are requiredwith other amino acids and labeling patterns.

Continued ➤Cambridge Isotope Laboratories, Inc.
North America: 1.800.322.1174 cilsales@isotope.com | International: +1.978.749.8000 intlsales@isotope.com | fax: 1.978.749.2768 | isotope.com

| SILAM Feeds

Cambridge Isotope Laboratories, Inc.

Other Products of Interest

Example References

Methionine (Met) Surrogates

Upadhyay, A.; Chhangani, D.; Rao, N.R.; et al. 2023. Amyloid fibril proteomics of AD
brains reveals modifiers of aggregation and toxicity. Mol Neurodegener, 18(1), 61-82

L-Azidohomoalanine·HCl (light, AHA; heavy, hAHA) andL-azidonorleucine·HCl (ANL) can be used to evaluate thesynthesis and turnover of newly synthesized proteins in vivo
through targeted or untargeted MS analysis (e.g., Yates JR et al.
JPR 2015). For immediate use in SILAM experiments, CIL offers
a collection of Mouse Express mouse feeds (see table below).
Please inquire for pricing.
Catalog No.
MF-AHA

Description
Mouse Express AHA Mouse Feed(contains 2 g of AHA per kg of mouse feed)
MF-HAHA
Mouse Express hAHA Mouse Feed(contains 2 g of hAHA per kg of mouse feed)
MF-UNLABELED-MET
Mouse Express Mouse Feed (unlabeled)(contains 2 g of L-Met per kg of mouse feed)
MLK-HAHA-KIT
Mouse Express hAHA Mouse Feed Kit (contains 1kg each of hAHA, AHA, and unlabeled Met feed)
MF-ANL
Mouse Express ANL Mouse Feed (unlabeled)(contains 20 g ANL per kg of mouse feed)
MF-ANL-NE-CONTROL* Mouse Express ANL Mouse Feed (unlabeled)(contains 2 g of L-Met per kg of mouse feed)*Non-essential (NE) amino acids increased proportionally, while keeping othermacronutrient sources (e.g., glucose, fat) constant, to compensate for 2% ANL
in MF-ANL.

Please visit isotope.com/silam for additional informationand complete product listings.

Fornasiero, E.F.; Savas, J. N. 2023. Determining and interpreting protein lifetimes inmammalian tissues. Trends Biochem Sci, 48(2), 106-118.
Wood, N.B.; Kelly, C.M.; O’Leary, T.S.; et al. 2022. Cardiac myosin filaments aremaintained by stochastic protein replacement. Mol Cell Proteomics, 100274-100289.
Novak, J.S.; Spathis, R.; Dang, U.J.; et al. 2021. Interrogation of Dystrophin andDystroglycan Complex Protein Turnover After Exon Skipping Therapy. J NeuromusculDis, 8(s2), S383-S402.
Hark, T.J.; Savas, J.N. 2021. Using stable isotope labeling to advance our understandingof Alzheimer's disease etiology and pathology. J Neurochem, 159(2), 318-329.
Ma, Y.; McClatchy, D.B.; Martínez-Bartolomé, S.; et al. 2021. Temporal quantitative
profiling of newly synthesized proteins during αβ accumulation. J Proteome Res, 20(1),763-775.
Hark, T.J.; Rao, N.R.; Castillon, C.; et al. 2020. Pulse-chase proteomics of the APP
knockin mouse models of Alzheimer’s disease reveals that synaptic dysfunction
originates in presynaptic terminals. Cell Syst, S2405-4712(20), 30458-30460.
Jongkamonwiwat, N.; Ramirez, M.A.; Edassery, S.; et al. 2020. Noise exposures causing
hearing loss generate proteotoxic stress and activate the proteostasis network. CellRep, 33(8), 108431.
Liu, P.; Xie, X.; Jin, J. 2020. Isotopic nitrogen-15 labeling of mice identified long-lived
proteins of the renal basement membranes. Sci Rep, 10(1), 5317.
Drigo, R.A.E.; Lev-Ram, V.; Tyagi, S.; et al. 2019. Age mosaicism across multiple scales
in adult tissues. Cell Metab, 30(2), 343-351.
Wallace, M.; Green, C.R.; Roberts, L.S.; et al. 2018. Enzyme promiscuity drives
branched-chain fatty acid synthesis in adipose tissues. Nat Chem Biol, 14(11),1021-1031.
Liu, P.; Thomson, B.R.; Khalatyan, N.; et al. 2018. Selective permeability of mouseblood-aqueous barrier as determined by 15N-heavy isotope tracing and mass
spectrometry. Proc Natl Acad Sci U S A, 115(36), 9032-9037.
Heo, S.; Diering, G.H.; Na, C.H.; et al. 2018. Identification of long-lived synaptic
proteins by proteomic analysis of synaptosome protein turnover. Proc Natl Acad Sci
U S A, 115(16), E3827-E3836.
Moody, L.R.; Barrett-Wilt, G.A.; Sussman, M.R.; et al. 2017. Glial fibrillary acidic protein
exhibits altered turnover kinetics in a mouse model of Alexander disease. J Biol Chem,292(14), 5814-5824.
McClatchy, D.B.; Ma, Y.; Liu, C.; et al. 2015. Pulsed azidohomoalanine labeling inmammals (PALM) detects changes in liver-specific LKB1 knockout mice. J Proteome
Res, 14(11), 4815-4822.
Zhang, A.; Uaesoontrachoon, K.; Shaughnessy, C.; et al. 2015. The use of urinary
and kidney SILAM proteomics to monitor kidney response to high dose morpholino
oligonucleotides in the mdx mouse. Toxicol Rep, 2, 838-849.
Hathout, Y.; Marathi, R.L.; Rayavarapu, S.; et al. 2014. Discovery of serum protein
biomarkers in the mdx mouse model and cross-species comparison to Duchenne
muscular dystrophy patients. Hum Mol Genet, 23(24), 6458-6469.
McClatchy, D.B.; Yates, J.R. III. 2014. Stable isotope labeling in mammals (SILAM).
Methods Mol Biol, 1156, 133-146.
Wan, J.; Savas, J.N.; Roth, A.F.; et al. 2013. Tracking brain palmitoylation change:
predominance of glial change in a mouse model of Huntington’s disease. Chem Biol,20(11), 1421-1434.
Rayavarapu, S.; Coley, W.; Cakir, E.; et al. 2013. Identification of disease specificpathways using in vivo SILAC proteomics in dystrophin deficient mdx mouse.
Mol Cell Proteomics, 12(5), 1061-1073.

Cambridge Isotope Laboratories, Inc., 3 Highwood Drive, Tewksbury, MA 01876 USA
tel: +1.978.749.8000

fax: +1.978.749.2768

1.800.322.1174 (North America)

www.isotope.com

PRT_SILAM_FEEDS (11/14/23)
Supersedes all previously published literature