Safety data sheet
Issuing date: 20Sep22

Revision date: 14Feb23

1.

Product and company identification

1.1

Product identifier
Product nameProduct number(s)Other means of identification

1.2

Restrictions on use

Onso™ fragmentation DNA library prep kit
102-499-100
This product is a kit box containing 8 reagent tubes.
Subcomponent name

Part ##Cap color

Fragmentation buffer

102-554-800

1

blue

Fragmentation enzyme

102-554-900

1purpleLigation mix

102-554-400

3yellow

Ligation enhancer

102-554-500

1

redEDTA

102-554-600

2whiteFor creation of Onso-compatible libraries from unfragmented, highmolecular weight DNA. Contains reagents for DNA fragmentation,end-repair, A-tailing, and adapter ligation of DNA fragments (96reactions).
Research use only. Professional users only.

Details of the supplier of the substance or mixture
Supplier name and address

Supplier phone
1.4

SDS PN: 102-784-200

Relevant identified uses of the substance or mixture and uses advised against
Identified use

1.3

Rev. 02

Pacific Biosciences of California, Inc.
1305 O’Brien Drive
Menlo Park, CA 94025
USA
https://www.pacb.com+1.650.521.8000

Emergency telephone number
Within USA and Canada

Call CHEMTREC 1-800-424-9300 (reference CCN# 656805)

Outside USA and Canada

techsupport@pacb.com

2.

Hazard(s) identification

2.1

Classification of the substance or mixture
The kit contains hazardous substances. 1 Each subcomponent is classified as follows.
Subcomponent name1

Part #Classification

Fragmentation buffer

102-554-800

Acute aquatic toxicity (category 3), Chronic aquatic toxicity (category 3).

Fragmentation enzyme

102-554-900

Acute aquatic toxicity (category 3), Chronic aquatic toxicity (category 3).

Ligation mix

102-554-400

Not a hazardous material.

Ligation enhancer

102-554-500

Not a hazardous material.

EDTA

102-554-600

Eye irritant (category 2A)

Classification in accordance with 29CFR1910 (OSHA HCS) and UN Globally Harmonized System of Classification and Labelling of Chemicals (GHS)

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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02

2.2

GHS label elements and precautionary statements
Information is provided for the hazardous components listed in section 2.1

Product

Pictogram

Signal word

Fragmentationbuffer

None

None

Fragmentation
enzyme

None

None

EDTA

2.3

Hazard statement(s)• Harmful to aquatic life.
• Harmful to aquatic life
with long-lasting effects• Harmful to aquatic life.
• Harmful to aquatic life
with long-lasting effects• Causes serious eyeirritation.

Warning

Precautionary statement(s)• Avoid release to the environment.
• Dispose of contents and container inaccordance with local regulations.
• Avoid release to the environment.
• Dispose of contents and container inaccordance with local regulations.
• Wash skin thoroughly after handling.
• Wear protective gloves/eye
protection/face protection.
• IF IN EYES: Rinse cautiously with water forseveral minutes. Remove contact lenses if
present and easy to do so.
• Is eye irritation persists: Get medicaladvice/attention. .

Hazards not otherwise classified (HNOC) or not covered by GHS
None.

3.

Composition / information on ingredients

3.1

Substances

3.2

Hazardous components

Common name and synonyms
CAS number
Other unique identifiers

Onso™ fragmentation DNA library prep kit
None
None

The following hazardous ingredients are present.
Subcomponent

Hazardous ingredient(s)CASFragmentationbuffer

Triton™ X-100

9036-19-5

Fragmentation
enzymeTriton™ X-100

9036-19-5

EDTA

Ethylenediaminetetraaceticacid60-00-4

4.

First aid measures

4.1

Description of first aid measures
General advice
If inhaled

Eye irritant (category 2A)

0.1-1%

0.1-1%> 15% and < 20%

If breathed in, move person into fresh air. If not breathing, give artificial respiration.

In case of skin contact

Wash off with water.

In case of eye contact

Flush eyes with water.

If swallowed

Acute toxicity, oral (category 4); Skin irritation(category 2); Serious eye damage (category 1);
Acute aquatic toxicity (category 1); Chronic
aquatic toxicity (category 1)
Acute toxicity, oral (category 4); Skin irritation(category 2); Serious eye damage (category 1);
Acute aquatic toxicity (category 1); Chronic
aquatic toxicity (category 1)

Concentration(wt%)

Consult a physician. Show this safety data sheet to the physician. . Follow the generalpractices listed for all component and specific practices as noted.

•4.2

GHS classification

EDTA: Rinse cautiously with water for several minutes. Remove contact lenses if
present and easy to do so. If eye irritation persists: Get medical advice/attention

Never give anything by mouth to an unconscious person. Rinse mouth with water.

Most important symptoms/effects, both acute and delayed
No data available. Refer to section 2.2 and section 11 for additional information.

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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02

4.2

Indication of any immediate medical attention and special treatment needed
No data available

5.

Fire-fighting measures

5.1

Suitable extinguishing media
Dry chemical, carbon dioxide, foam, or water.

5.2

Special hazards arising from the substance or mixture
Combustion will produce oxides of carbon and nitrogen.

5.3

Advice for firefighters (special protective equipment or precautions)Wear self-contained breathing apparatus for firefighting as necessary.

6.

Accidental release measures

6.1

Personal precautions, protective equipment and emergency procedures
Avoid breathing vapors, mists, or gas. For personal protection see section 8.0.

6.2

Environmental precautions
Avoid release to the environment. Report spills and releases as required to appropriate authorities.

6.3

Methods and materials for cleanup
Wear personal protective equipment, wipe up with paper towel (or similar), and contain in closed containers.

6.4

Reference to other sections
For disposal see section 13.

7.

Handling and storage

7.1

Precautions for safe handling
For all components, utilize standard good lab practices, use in a well-ventilated area, and wear standard PPE(gloves, lab coat, safety glasses). In addition to the general practices, follow specific practices as noted.
•7.2

EDTA: Wash skin thoroughly after handling. Wear protection gloves/eye protection/face protection.

Conditions for safe storage, including any incompatibilities
Keep containers tightly closed at the recommended storage temperature.

8.

Exposure controls / personal protection

8.1

Control parameters
Contains no substances with occupational exposure limit values.

8.2

Exposure controls
Appropriate engineering controls:
General industrial hygiene practice. No special ventilation required for normal use.
Personal protective equipment (PPE):
Eye/face protection
Skin and body protection
Respiratory protection

Safety glasses
Latex or nitrile gloves; lab coat /apron
None required

9.

Physical and chemical properties9.1

Information on basic physical and chemical properties
Appearance
Odor
Odor threshold

Each reagent tube contains clear colorless liquid.
No odorpH

No data available
7.0-9.0

Melting point/freezing point

No data available

Initial boiling point and boiling range

No data available

Flash point

No data available
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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02

Evaporation rate

No data available

Flammability (solid, gas)

No data available

Upper/lower flammability or explosive limit

No data availableVapor pressure

No data available

Relative density

No data available

Solubility(ies)

No data available

Partition coefficient: n-octanol/water

No data available

Auto-ignition temperature

No data available

Decomposition temperature

No data available

Viscosity

No data available

Reactivity

This product is not reactive under normal conditions of storage and use.

10.

Stability and reactivity
Chemical stability

Stable under recommended storage conditions.

Possibility of hazardous reactions

No data available

Conditions to avoid

No data available

Incompatible materials
Hazardous decomposition products

Avoid contact with strong acids, bases, and oxidizers.
No data available. In the event of fire see section 5.

11.

Toxicological information

11.1

Information on likely routes of exposure
The most likely route of exposure is via skin or eye contact. Exposure via inhalation or ingestion is less likely.

11.2

Symptoms related to the physical, chemical, and toxicological characteristics
No data available

11.3

11.4

11.5

Delayed and immediate effects, and chronic effects from short-and long-term exposure
Eyes

No data available

Skin

No data available

Inhalation

No data availableIngestion

No data available

Chronic effects

No data available

Aggravated medical conditions

No data available

Interactions with other chemicals

No data available

Numerical measures of toxicity
Acute toxicity

No data available

Skin corrosion/irritation

No data available

Serious eye damage/irritation

No data available

Respiratory or skin sensitization

No data available

Germ cell mutagenicity

No data available

Reproductive toxicity

No data available

Specific target organ toxicity

No data available

Carcinogenicity
No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP or OSHA.

12.

Ecological information
Ecotoxicity

No data available

Persistence and degradability

No data available

Bioaccumulative potential

No data available

Mobility in soil

No data available
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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02

Results of PBT and vPvB assessment
Other adverse effects

13.

None Required
No data available

Disposal considerations
Dispose all waste in accordance with local, regional, and national regulations.
ProductSee above

Contaminated packaging

See above

US EPA waste number

14.

No EPA waste numbers apply. Not regulated as a US federal hazardouswaste. May be regulated under individual state laws.

Transport information
Transportation of this product is not regulated under ICAO, IMDG or US DOT.

15.

Regulatory information

15.1

USA federal regulations
SARA 302

No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302

SARA 313

This material does not contain any chemical components with known CAS numbers that exceed thethreshold reporting levels established by SARA Title III, Section 313

SARA 311/312
TSCA
15.2

EDTA: Specific target organ toxicity (repeat exposure)
Research use only.

USA state regulations
CA Prop 65

This kit does not contain any chemicals known to the state of California to cause cancer or adverse
reproductive health effects

16. Other information
Prepared by

Pacific Biosciences of California, Inc.
Environment, Health and Safety
1305 O’Brien Drive
Menlo Park, CA 94025
USAsafety@pacb.com

Furtherinformation

The above information is believed to be correct but does not purport to be all inclusive and shall be
used only as a guide. The information in this document is based on the present state of ourknowledge and is applicable to the product with regard to appropriate safety precautions. It does not
represent any guarantee of the properties of the product. It is not a warranty or quality specification.
This information relates only to the specific material designated and may not be valid for use incombination with any other material or in any other process.
Research use only. Not for use in diagnostic procedures. ©2023, Pacific Biosciences of California, Inc.
(“PacBio”). All rights reserved. Information in this document is subject to change without notice.
PacBio assumes no responsibility for any errors or omissions in this document. Certain notices, terms,conditions and/or use restrictions may pertain to your use of PacBio products and/or third-party
products. Refer to the applicable PacBio terms and conditions of sale and to the applicable license
terms at pacb.com/license. Pacific Biosciences, the PacBio logo, PacBio, Circulomics, Omniome,SMRT, SMRTbell, Iso-Seq, Sequel, Nanobind, SBB, Revio, and Onso are trademarks of PacBio.

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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02