Quality Results for life

PRODUCT CATALOGUEEmbryoNida®
〇 EmbryoNida® is hyaluronan rich (0.5 mg/mL) medium for embryo transfer.
Hyaluronic acid is a substance present in skin, endometrium
and follicles, which facilitates binding of the embryo to theendometrium and provides a physical protective effect.
Hyaluronic acid used in EmbryoNida® is of high-purity
pharmaceutical-grade, with low molecular weight.
〇 EmbryoNida® is for both cleavage-stage embryos and blastocysts.
〇 EN for use in multi gas incubator.
〇 ENM is HEPES buffered.

10mLREF

CodeDescription

Contents

93720

EN-10

ET Medium EmbryoNida

10mL×293721

EN-1.0ET Medium EmbryoNida

1.0mL×1093722

ENM-10ET Medium EmbryoNida with HEPES

10mL×293723

ENM-1.0

ET Medium EmbryoNida with HEPES

1.0mL×101.0mL

COMPONENTSQUALITY CONTROL

Alanyl-L-glutamine / Amino Acids / Calcium Chloride /pH 7.2-7.6 /Gentamicin / Glucose / Human Serum Albumin /Osmolarity 270-295mOsm/L / Endotoxin <0.25EU/mL /Hyaluronic acid / Magnesium Sulfate / Potassium Chloride /Sterility test / Mouse Embryo Assay ≥80%

Potassium Phosphate / Sodium Bicarbonate / Sodium Chloride /Sodium Lactate / Sodium Pyruvate

Storage: 2-8℃

"M" in the code : with HEPES

Shelf life: EN4 months: ENM 8 months

RESULTContents

EmbryoNida®

Frozen Embryo Transfer Cycle

237

Average age

35.8±4.3

Average No. of Embryo Transfer

2.3±2.2

Implantation rate

42.6%(101/237)

Abortion rate

11.1%(9/81)

From St. Mother Hospital Infertility Clinic

Specification may change without pre-notice for purpose of product improvement.

Kitazato Corporation
HEADQUATER : 100-10 Yanagishima, Fuji, Shizuoka 416-0932 JAPANTOKYO : 1-1-8 Shibadaimon, Minato-ku, Tokyo 105-0012 JAPAN
Mail

contact@kitazato.co.jp
Copyright© Kitazato Corporation All Rights Reserved. | v.5_01 2024.10.23

PROTOCOL* Perform embryo transfers following your laboratoy protocol based on your expertise and proven results.
Below is an example of protocol using respective ET medium.

For use of ENM (with HEPES)

01
02
03

：Prepare a dish for ET with 1mL of ENM prewarmed to 37℃.
Then, transfer embryos from culture dish to the ET dish.

Preparation

Embryo loading ：Load embryos with Kitazato ET catheter under a stereomicroscope,by aspirating air, embryo and air, in this order.
Embryo transfer ：Introduce the Kitazato ET catheter to the patient's uterus and place the embryos there.

ENM (with HEPES)

Stereo microscope

Warm at 37℃ in an incubator
Pre-warmed ENM

ENM 1mLEN (without HEPES)
Equilibrate at 37℃,
5% CO2 incubator ≥2hEmbryo

56

Equilibrated EN

7air

ENM/ENairET catheter
105EN 1mL in a dish for ET

6EmbryoPreparation / culture before embryo transfer

Embryo loading

Embryo transfer

For use of EN (without HEPES)

01Preparation：Prepare a dish for ET with 1mL of EN.
Then, equilibrate at 37℃, 5% CO2 or similar environment in an incubator for a minimumo of 2 hours.

02

Culture before embryo transfer ：（A）For vitrified embryos, use EN for recovery culture after warming.
（B）
For fresh embryos, additional culture in EN is not mandatory, but they can be kept in EN
if selecting embryos before transfer. Refrain from overnight or long time culture.

03

Preparation for Embryo Transfer：（A）Take out the embryos cultured after warming from the incubator.
（B）Transfer the cultured fresh embryos to the EN.

04

Embryo loading ：Load embryos with Kitazato ET catheter under a stereomicroscope,by aspirating air, embryo and air, in this order.

05

Embryo transfer ：Introduce the Kitazato ET catheter to the patient's uterus and place the embryos there.

RELATED PRODUCTS〇 Dish for culture：35mm 9dimple dish (REF. 1502050), Center well plate (REF. 1502040)〇 Dish for embryo transfer：Culture dish (⏀35mm) (REF. 1502000), Center well dish (REF. 1502045)
5well multi plate (REF. 1502030), Center well dish EZ (REF. 1502055)