Safety data sheet
Issuing date: 25Jan23

Revision date: 25Jan23

1.

Product and company identification

1.1

Product identifier
Product nameProduct number(s)Other means of identification

1.2

Restrictions on useNanobind® HT CBB kit RT
102-762-900
This product is a kit box containing 7 reagent tubes.
Component name

Part ##Cap color

Proteinase K (PK)102-783-100

1

Clear

Nanobind® disks102-783-600

1

Clear

Buffer EB

102-782-800

1

Clear

Buffer CLE3

102-783-200

1

Clear

Buffer BL3

102-783-300

1ClearBuffer CW1

102-783-400

1ClearBuffer CW2

102-783-500

1ClearThis kit is used in conjunction with Nanobind® HT CBB kit 4C (102763-000) to complete automated HMW DNA extraction from cells,
bacteria, and blood (96 reactions)
Research use only.

Details of the supplier of the substance or mixture
Supplier name and address

Supplier phone
1.4

SDS PN: 102-936-700

Relevant identified uses of the substance or mixture and uses advised against
Identified use

1.3

Rev. 01

Pacific Biosciences of California, Inc.
1305 O’Brien Drive
Menlo Park, CA 94025
USA
https://www.pacb.com+1.650.521.8000

Emergency telephone numberWithin USA and Canada

Call CHEMTREC 1-800-424-9300 (reference CCN# 656805)

Outside USA and Canada

techsupport@pacb.com

2.

Hazard(s) identification

2.1

Classification of the substance or mixture
The kit contains hazardous substances. 1 Each component is classified as follows.
Proteinase K (PK)1102-783-100

Skin irritation (category 3), Respiratory sensitizer (category 1)

Nanobind® disks102-783-600

Not a hazardous material.

Buffer EB

102-782-800

Not a hazardous material.

Buffer CLE3

102-783-200

Skin irritation (category 3), Eye irritation (category 2), Acute aquatic
toxicity (category 3).

Classification in accordance with 29CFR1910 (OSHA HCS) and UN Globally Harmonized System of Classification and Labelling of Chemicals (GHS)

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2.2

Buffer BL3

102-783-300

Acute toxicity, oral (category 4), Acute toxicity, inhalation (category 4),
Skin irritation (category 2), Eye irritation (category 2A), Acute aquatic
toxicity (category 3)

Buffer CW1

102-783-400

Skin irritation (category 3), Acute aquatic toxicity (category 3), Chronic
aquatic toxicity (category 3)

Buffer CW2

102-783-500

Not a hazardous material.

GHS label elements and precautionary statements
Information is provided for the hazardous components listed in section 2.1

Component

PictogramProteinase K (PK)

Buffer CLE3

Buffer BL3

Buffer CW1

None

Signal word

Hazard statement(s)Danger• Causes mild skin irritation.
• May cause allergy orasthma symptoms or
breathing difficulties if
inhaled.

Warning• Causes serious eye
irritation.
• Causes mild skin irritation.
• Harmful to aquatic life.

Warning• Harmful if swallowed.
• Harmful if inhaled.
• Causes serious eyeirritation.
• Causes skin irritation.
• Harmful to aquatic life.

Warning• Causes mild skin irritation.
• Harmful to aquatic life
• Harmful to aquatic life
with long lasting effects

Precautionary statement(s)• If skin irritation occurs, get medicalattention.
• Avoid breathing mist, vapors, or spray.
• In case of inadequate ventilation, wear
respiratory protection.
• If inhaled, remove person to fresh air andkeep comfortable for breathing.
• If experiencing respiratory symptoms, getemergency medical help immediately.
• Dispose of contents and container inaccordance with local regulations.
• Wash hands thoroughly after handling.
• Wear eye protection.
• Avoid release to the environment.
• If in eye, rinse cautiously with water forseveral minutes. Remove contact lenses if
present and easy to do. Continue rinsing.
• If eye irritation persists, get medical advice.
• If skin irritation occurs, get medical help.
• Dispose of contents and container inaccordance with local regulations.
• Do not eat, drink, or smoke when using thisproduct.
• Use in well-ventilated area.
• Wear protective gloves and eye protection.
• Avoid breathing mist, vapors, or spray.
• Wash hands thoroughly after handling.
• If swallowed, rinse mouth and call poison
center or doctor if you feel unwell.
• If inhaled, remove person to fresh air andkeep comfortable for breathing. Call poison
center or doctor if you feel unwell.
• If on skin, wash with plenty of water. If skinirritation occurs, get medical attention.
• If in eye, rinse cautiously for several
minutes. Remove contact lenses if presentand easy to do. Continue rinsing. If eyeirritation persists, get medical advice.
• Take off contaminated clothing and wash
before reuse.
• Dispose of contents and container inaccordance with local regulations.
• If skin irritation occurs, get medicalattention.
• Avoid release to the environment.
• Dispose of contents and container inaccordance with local regulations.

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2.3

Hazards not otherwise classified (HNOC) or not covered by GHS
Proteinase K is of biological origin. It is produced by the fungus Tritirachium album Limber.

3.

Composition / information on ingredients

3.1

Substances
Common name and synonyms
CAS number
Other unique identifiers

3.2

Hazardous components

Nanobind® CBB kit RT
None
None

The following hazardous ingredients are present.
Component

Hazardous ingredient(s)CASProteinase K (PK)Proteinase K

39450-01-6

Buffer CLE3

Trade secretTrade secret

Buffer BL3

Guanidine hydrochloride

50-01-1

Buffer CW1

Guanidine hydrochloride

50-01-1

Buffer CW1

Trade secretTrade secret4.

First aid measures

4.1

Description of first aid measures
General advice
If inhaled

GHS classification
Eye irritation (category 2), Skin irritation (category2A), Respiratory sensitizer (category 1), Specifictarget organ toxicity – single exposure (category 3)
Acute Toxicity, oral (category 4), Acute toxicity,
inhalation (category 4), Skin irritation (category 2),
Serious eye damage (category 1), Specific target
organ toxicity, respiratory – single exposure(category 3), Acute aquatic toxicity (category 2),
Chronic aquatic toxicity (category 3)
Acute Toxicity, oral (category 4), Acute toxicity,
inhalation (category 4), Skin irritation (category 2),
Eye irritation (category 2A), Acute aquatic toxicity(category 3)
Acute Toxicity, oral (category 4), Acute toxicity,inhalation (category 4), Skin irritation (category 2),
Eye irritation (category 2A), Acute aquatic toxicity(category 3)
Acute Toxicity, oral (category 4), Skin irritation(category 2), Serious eye damage (category 1),
Acute aquatic toxicity (category 1), Chronic aquatic
toxicity (category 1)

Concentration
(wt%)
2%

2.9%

76%

7%

0.75%

Consult a physician. Show this safety data sheet to the physician. Follow the generalpractices listed for all components and specific practices as noted.
If breathed in, move person into fresh air and keep comfortable for breathing. If notbreathing, give artificial respiration.
••

PK: If experiencing respiratory symptoms, get emergency medical help immediately.
BL3: Call poison center or doctor if you feel unwell.

In case of skin contact

Wash off with water.

In case of eye contact

Flush eyes with water.
•CLE3: If in eye, rinse cautiously for several minutes. Remove contact lenses if presentand easy to do. Continue rinsing. If eye irritation persists, get medical advice.
•BL3: If in eye, rinse cautiously for several minutes. Remove contact lenses if present andeasy to do. Continue rinsing. If eye irritation persists, get medical advice.

If swallowed

•
•
•
•

PK: If skin irritation occurs, get medical attention.
CL3: If skin irritation occurs, get medical attention.
BL3: Wash with plenty of water. If skin irritation occurs, get medical attention.
CW1: If skin irritation occurs, get medical attention.

Never give anything by mouth to an unconscious person. Rinse mouth with water.
•BL3: If swallowed, rinse mouth and call poison center or doctor if you feel unwell.

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4.2

Most important symptoms/effects, both acute and delayed
No data available. Refer to section 2.2 and section 11 for additional information.

4.2

Indication of any immediate medical attention and special treatment needed
No data available

5.

Fire-fighting measures

5.1

Suitable extinguishing media
Dry chemical, carbon dioxide, foam, or water.

5.2

Special hazards arising from the substance or mixture
Combustion will produce oxides of carbon and nitrogen. Fire may produce irritating gas. Runoff may pollutewaterways.

5.3

Advice for firefighters (special protective equipment or precautions)Wear self-contained breathing apparatus for fire-fighting as necessary.

6.

Accidental release measures

6.1

Personal precautions, protective equipment and emergency procedures
Avoid breathing vapors, mists, or gas. For personal protection see section 8.0.

6.2

Environmental precautions
Avoid release to the environment. Report spills and releases as required to appropriate authorities.

6.3

Methods and materials for cleanup
Wear personal protective equipment, wipe up with paper towel (or similar), and contain in closed containers.

6.4

Reference to other sections
For disposal see section 13.

7.

Handling and storage

7.1

Precautions for safe handling
For all components, utilize standard good lab practices, use in a well-ventilated area, and wear standard PPE(gloves, labcoat, safety glasses). In addition to the general practices, follow specific practices as noted.
••
•

7.2

PK: Avoid breathing mist, vapors, or spray. In case of inadequate ventilation, wear respiratory protection.
CLE3: Wash hands thoroughly after handling.
BL3: Do not eat, drink, or smoke when using this product. Avoid breathing mist, vapors, or spray. Wash hands
thoroughly after handling.

Conditions for safe storage, including any incompatibilities
Keep containers tightly closed at the recommended storage temperature.

8.
8.1

Exposure controls / personal protection
Control parametersContains no substances with occupational exposure limit values.

8.2

Exposure controls
Appropriate engineering and administrative controls:
General industrial hygiene practice. No special ventilation required for normal use.
•BL3: Take off contaminate clothing and wash before reuse.

Personal protective equipment (PPE):
Eye/face protection
Skin and body protection
Respiratory protection

Safety glasses
Latex or nitrile gloves; lab coat /apron
None required

9.

Physical and chemical properties

9.1

Information on basic physical and chemical properties
Appearance

Each reagent tube contains clear colorless liquid. BL3 may appear slightly
yellowish.
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P/N: 101-648-100, Rev03
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OdorOdor thresholdNo odorpH

No data available
7.5-9

Melting point/freezing point

No data available

Initial boiling point and boiling range

No data available

Flash point

No data available

Evaporation rate

No data available

Flammability (solid, gas)

No data available

Upper/lower flammability or explosivelimit

No data available

Vapor pressure

No data available

Relative density

No data available

Solubility(ies)

No data available

Partition coefficient: n-octonal/water

No data available

10.

Auto-ignition temperature

No data available

Decomposition temperature

No data available

Viscosity

No data available

Stability and reactivity
Reactivity
Chemical stability

This product is not reactive under normal conditions of storage and use.
Stable under recommended storage conditions.

Possibility of hazardous reactions

No data available

Conditions to avoid

No data available

Incompatible materials
Hazardous decomposition products

Avoid contact with strong acids, bases and oxidizers.
No data available. In the event of fire see section 5.

11.

Toxicological information

11.1

Information on likely routes of exposure
The most likely route of exposure is via skin or eye contact. Exposure via inhalation or ingestion is less likely.

11.2

Symptoms related to the physical, chemical and toxicological characteristics
No data available

11.3

11.4

Delayed and immediate effects and also chronic effects from short-and long-term exposure
Eyes

No data available

Skin

No data available

Inhalation

No data available

Ingestion

No data available

Chronic effects

No data availableAggravated medical conditions

No data available

Interactions with other chemicals

No data available

Numerical measures of toxicity (include calculated values?)
Acute toxicity

No data available

Skin corrosion/irritation

No data available

Serious eye damage/irritation

No data available

Respiratory or skin sensitization

No data available

Germ cell mutagenicity

No data available

Reproductive toxicity

No data available

Specific target organ toxicity

No data available
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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02

11.5Carcinogenicity
No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP or OSHA.

12.

Ecological information
Ecotoxicity

No data available

Persistence and degradability

No data available

Bioaccumulative potential

No data available

Mobility in soil

No data available

Results of PBT and vPvB assessment
Other adverse effects

13.

None Required
No data available

Disposal considerations
Dispose all waste in accordance with local, regional and national regulations.
ProductSee above.

Contaminated packaging

See above.

US EPA waste number

14.

No EPA waste numbers apply. Not regulated as a US federal hazardous
waste. May be regulated under individual state laws.

Transport information
Transportation of this product is not regulated under ICAO, IMDG or US DOT.

15.

Regulatory information

15.1

USA federal regulations
SARA 302

No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.

SARA 313

This material does not contain any chemical components with known CAS numbers that exceed thethreshold reporting levels established by SARA Title III, Section 313.

SARA 311/312

PK: Respiratory Sensitizer.
CLE3: Eye irritation.
BL3: Acute toxicity (oral), Acute toxicity (inhalation), Eye irritation, Skin irritation.

TSCA
15.2

Research use only

USA state regulations
CA Prop 65

This kit does not contain any chemicals known to the state of California to cause cancer or adverse
reproductive health effects

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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev0216. Other information
Prepared byPacific Biosciences of California, Inc.
Environment, Health and Safety
1305 O’Brien Drive
Menlo Park, CA 94025
USA
safety@pacb.com

Furtherinformation

The above information is believed to be correct but does not purport to be all inclusive and shall be
used only as a guide. The information in this document is based on the present state of our knowledge
and is applicable to the product with regard to appropriate safety precautions. It does not represent any
guarantee of the properties of the product. It is not a warranty or quality specification. This information
relates only to the specific material designated and may not be valid for use in combination with anyother material or in any other process.
Research use only. Not for use in diagnostic procedures. © 2023 Pacific Biosciences of California, Inc.
(“PacBio”). All rights reserved. Information in this document is subject to change without notice. PacBio
assumes no responsibility for any errors or omissions in this document. Certain notices, terms,
conditions and/or use restrictions may pertain to your use of PacBio products and/or third-party
products. Refer to the applicable PacBio terms and conditions of sale and to the applicable license
terms at pacb.com/license. Pacific Biosciences, the PacBio logo, PacBio, Circulomics, Omniome, SMRT,SMRTbell, Iso-Seq, Sequel, Nanobind, SBB, Revio, and Onso are trademarks of PacBio.

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P/N: 101-648-100, Rev03
Governing procedure: 101-647-900 WI, Creation of Product Safety Data Sheet Rev02