Athens Research & Technology
110 Trans Tech Drive Athens, GA 30601 USA
PHONE: 706-546-0207 • FAX: 706-546-7395 • EMAIL: ARTBIO@ATHENSRESEARCH.COM

www.athensresearch.com

Gel Scan of Representative Lot
Lipoproteins, High Density, Human Plasma, Suitable for Cell Culture
ART Catalog No. 12-16-080412-TC

Athens Research & Technology

97.4 kDa
66.2 kDa
45.0 kDa

31.0 kDa

21.5 kDa
14.4 kDa

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

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12SDS-PAGE:
12% BIO-RAD Tris HCl gel
1.
2.

LMW Standard (BIO-RAD Cat. No. 161-0304)
HDL - 10 µg (reduced / heat)

Molecular Weight: 175,000-360,000
Protein Determination:
Modified Lowry Based on BSA as standard

Considerations:
Storage Conditions: 2-6o C, away from light
Do Not Freeze

Density: 1.063-1.210 g/ml;
Composition: 55-45% lipid, 45-55% Protein

Stability: Open under a laminar flow hood<= 7 days once opened

Sterility Testing:

Buffer: 150 mM NaCl, pH 7.4, 0.01% EDTA

Using Turbidity Growth/No Growth as the Indicator
Thioglycollate Broth (THIO) for yeast and mold growth
Tryptic Soy Broth (TSB) for bacterial growth

Endotoxin Level: <= 10 EU/mg by Pyrosate LAL clot test
Physical Specifications:
LiquidPurity:

Single arc by IEP against antisera to whole human serum. Essentially freeof other plasma lipoproteins as determined by electrophoresis using a
SPIFE Vis Cholesterol gel kit for lipids and Coomassie Blue for proteins.
Greater than 95% of total lipoprotein content by electrophoresis.
Virginia B. Patel PhD

Athens Research & Technology
110 Trans Tech Drive Athens, GA 30601 USA
PHONE: 706-546-0207 • FAX: 706-546-7395 • EMAIL: ARTBIO@ATHENSRESEARCH.COM

www.athensresearch.com

Product Datasheet
Product:Lipoproteins, High Density, Human Plasma, Suitable for Cell Culture

Catalog Number:

12-16-080412-TC

Description:

LiquidBuffer:

150 mM NaCl, pH 7.4, and 0.01% EDTA

Purity:Single arc by IEP against antisera to whole human serum. Essentially freeof other plasma lipoproteins as determined by electrophoresis using aSPIFE Vis Cholesterol gel kit for lipids and Coomassie Blue for proteins.
Greater than 95% of total lipoprotein content by electrophoresis.

Sterility Testing:

Using Turbidity Growth/No Growth as the Indicator
Thioglycollate Broth (THIO) for yeast and mold growth
Tryptic Soy Broth (TSB) for bacterial growth

Molecular Weight:

MW 175,000-360,000

Density:

1.063-1.210 g/ml

Composition:

55-45% lipid, 45-55% protein

Endotoxin Level:<= 10 EU/mg by Pyrosate LAL clot test

Protein Determination:

Modified Lowry Based on BSA as standard

Storage:

2-6o C, away from light, Do Not Freeze

Stability:

Open under a laminar flow hood<= 7 days once opened

Available Packaging:

1 mg per vial
Larger aliquots upon request

Source:Human Plasma

Testing:

Shown to be non reactive for HBsAg, anti-HCV, anti-HBc,
and negative for anti-HIV 1 & 2 by FDA approved tests.

Athens Research & Technology products are laboratory reagents and are not to beadministered to humans or used for any drug purpose.
For research or further manufacturing use only.

Athens Research & Technology
110 Trans Tech Drive Athens, GA 30601 USA
PHONE: 706-546-0207 • FAX: 706-546-7395 • EMAIL: ARTBIO@ATHENSRESEARCH.COM

www.athensresearch.com
ISO9001:2008

Certificate Of Origin
Product Name:

Lipoproteins, High Density, Human Plasma, Tissue Culture

Catalog Number:

12-16-080412-TC

Lot Number:Information applies to all lots produced.

Manufacturing Information:

The methods and controls used in this facility for
manufacturing, processing, packaging and holding ofour products are in accordance with ISO9001:2008
Quality Management System.

Manufacturing Site:

Athens Research & Technology Inc., Athens, Georgia, USA

Raw Material Information:

The product contains no materials directly of animal origin
and has not been in contact with products of animal origin
in the course of its purification.

Country of Origin (manufacturing):

United States

Biological Source Material (COO):

United States

Donor Consent:

The raw material used in the purification of this product has been obtained from an
FDA licensed collection center. Further the donor or the donor’s next of kin has given
by signature full informed consent for the material to be used in commercial scientificresearch, including the sharing, transferring, or selling of samples to third parties
involved in this research. The raw material was obtained in an ethical manner in fullcompliance with national legislation, and the donor identity is held as confidential
information by the licensed collection center.

Athens Research & Technology products are laboratory reagents and are not to beadministered to humans or used for any drug purpose.
For research use or for use in further manufacturing.