Premier Solution for Automated
3D Confocal Raman Imaging

alpha300 apyron
Optimized Performance and Workflow

Next
Generationwww.witec.deapyron

Automation helps during every stepof Raman imaging measurementswith AutoBeam opto-mechanical components

The alpha300 apyron is the top of the line in WITec’sseries of Raman imaging microscopes. It has beendeveloped to combine ease-of-use and ultimate capability
by automating hardware control and offering preconfigured measurement routines. This streamlines theexperimental workflow and generates reproducible resultswith unrivaled speed, sensitivity and resolution.

NEWSetup• TrueCal executes pre-configured calibration routines.
• Laser safety class 1 (or 1M) compliant.
• AutoBeam concept for automatic and motorized beam path alignment includes:> Motorized 6-position objective turret: positions selected objective and compensates offsets> Motorized illumination selector: switches between all microscopy illumination options> Motorized laser coupler: delivers up to 6 laser wavelengths from UV to NIR> AutoBeam output coupler: automatically optimizes signal & resolution and selects
spectrometers> Motorized calibration source: fast & automatic multi-point spectrometer calibration
over the full spectral range> Motorized Köhler illumination apertures: facilitate focusing and optimize contrast andhomogeneity> Motorized polarization modules: freely rotatable automated excitation polarizer anddetection analyzer

Raman imaging

apyron [άπειρον] is derived from the ancient Greek word for infinity• TruePower determines absolute laser power with <0.1 mW accuracy.
• Instrument positions and repositions sample automatically withmotorized and piezo-driven scanning stages.
• Focus stabilization actively maintains focus during entire measurements.
• Motorized objective turret includes offset-compensation forconvenient precision; compatible with AFM/SNOM objectives.
• EasyLink handheld controller offers intuitive instrument operation.

Raman image of a cut agate with various phases of silica and silicon oxides
identified by their Raman spectra.

Extensive automation

True confocality and wavelengthoptimized design

Data processing, export
and display

High spatial and spectral resolution
simultaneously: CCl4 in an emulsion• Simplifies operation of the instrument.
• Requires less human input.
• Reduces potential sources of error.
• Enables complete remote control for use in environmental
enclosures such as glove boxes.
• Enhances reproducibility.

• Guarantees maximum throughput.
• Provides sharp images with resolution limited only by physical law.
• Yields spectral resolution down to 0.1 relative
wavenumbers/cm (@633 nm).
• Supports FAST RAMAN IMAGING without sacrificing resolution.

• Suite FIVE software wizard guides the user
through the processing of Raman spectra, frombackground reduction through image generation.
• TrueMatch software helps to identify moleculesby comparing measured spectra with existing
databases.
• Suite FIVE facilitates the export and display of
your data.

AB(A) 3D confocal Raman image of an emulsion, with a zoomed-in view ofthe inset. (B) shows the corresponding spectra: Green: alkane; blue: water;yellow: CCl4 and oil. Due to the high spectral resolution of the spectroscopic
system, the CCI4 band at 460 relative wavenumbers (cm-1) can be resolved atroom temperature into three peaks, as shown in the zoom-in. These peaks
reflect the isotopic variations of the molecules.

Image parameters: 200 x 200 x 20 pixels, 100 x 100 x 10 μm3 scan range,
6 ms integration time per spectrum, 532 nm excitation wavelength.

What automation means
The alpha300 apyron’s automation supports users during every step of the imaging process,making it the ideal Raman microscope for:• Multi-user laboratories with varying requirements and levels of user experience.
• Researchers employing remote operation, such as in enclosed environments.
• Raman newcomers with advanced imaging requirements.

• Veteran Raman microscopists seeking the next performance benchmark.

• Industry facilities with recurring experimental situations and an emphasis on
time-critical turnover.

Raman image of a moisturizing shower gel with increased oil content.
Red: oil; yellow: emulsifier; blue and cyan: aqueous phases.

The alpha300 apyron offers unrivaled dataacquisition speedAB

CD

The extremely high performance and speed of the alpha300 apyron
microscope is vividly demonstrated with the analysis of a tungsten
diselenide (WSe2) sample.
(A) In about 2 minutes, a clear and informative 75 x 75 μm2 Raman imageof the WSe2 flake was recorded. The flake consists of single layer areas(red), double layers (green) and multi-layers (blue). (B) shows the samemeasurement after smoothing. (C) A measurement of about 17 minutes
produced an even sharper image. The integration time was 6 ms per pixelin both experiments. The increased signal to noise ratio was achievedby reducing the pixel size from 750 nm in (A) to 230 nm in (C). In (D) thephotoluminescence image of the same flake is presented, corresponding
perfectly with the Raman image. (E) White-light image of the sample.

EWe take care
WITec uses environmentally friendly printed materials. While this policy
is only a small contribution to a healthy environment, we at WITec believethat focusing on details can effect positive change in the world.

WITec alpha300 seriesalpha300
accessalpha300 R
Confocal Raman
Microscopealpha300 Ri
Inverted Confocal
Raman Microscope

RISE™
Correlative
Raman-SEM Imaging

alpha300 apyron™
Automated Confocal Raman Miroscope

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Fax +49 (0) 731 14070200
info@witec.de
www.witec.de

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Phone +1 865 984 4445
Fax +1 865 984 4441
info@witec-instruments.com
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Phone +65 9026 5667
shawn.lee@witec.biz
www.witec.de

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WITec Beijing Representative Office
Unit 1307A, Air China Plaza Tower 1No. 36 Xiaoyun Road
Beijing, PRC., 100027
Phone +86 (0) 10 6590 0577
info.china@witec-instruments.com
www.witec.de/cn

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1-1-5 Furo-cho, Naka-ku,
Yokohama City, Kanagawa Pref. 231-0032
JapanPhone +81 45 319 4277
info@witec.jp
www.witec.de/jp