➨ 㸳 ❶            

ே␆ඹ㏻ឤᰁ⑕ࣞࣉࢺࢫࣆࣛ⑕ࡢឤᰁ≧ἣせ Ⅼ࣭ࣞࣉࢺࢫࣆࣛ⑕ࡣ Leptospira interrogans㸦⑓ཎᛶ⣽⳦㸧ឤᰁ࡟㉳ᅉࡍࡿே␆ඹ㏻ឤ ᰁ⑕࡛࠶ࡿࠋ࣭ࣞࣉࢺࢫࣆࣛࡢಖ⳦ື≀ࡣࡆࡗṑ㢮ࢆࡣࡌࡵ࡜ࡋࡓ㔝⏕ື≀࡛࠶ࡾࠊ㏆ᖺࠊಶయᩘࡀᛴ⃭࡟ቑຍࡋࠊேࡢ⏕άᅪ࡟ฟἐࡍࡿᶵ఍ࡢቑ࠼࡚࠸ࡿ࢔ࣛ࢖ࢢ࣐ࡶࡲࡓࠊࣞࣉࢺࢫࣆࣛࡢಖ⳦ື≀࡜࡞ࡿྍ⬟ᛶࡢ࠶ࡿື≀࡛࠶ࡿࠋ࣭࢔ࣛ࢖ࢢ࣐㜵㝖ィ⏬ࡀᐇ᪋ࡉࢀ࡚࠸ࡿරᗜ┴ෆࡢ࢔ࣛ࢖ࢢ࣐࡟࠾࠸࡚ࠊ㢧ᚤ㙾ୗจ㞟ヨ㦂㸦MAT㸧ࢆ⏝࠸࡚ࣞࣉࢺࢫࣆࣛᢠయಖ᭷⋡ࡢㄪᰝࢆ⾜࠺࡜࡜ࡶ࡟ PCR ࢆ⏝࠸࡚ ࣞࣉࢺࢫࣆࣛ㑇ఏᏊࢆ᳨ฟࡋࡓࠋ࣭୺࡟ศᕸ୰ᚰ࡟࠾࠸࡚ᅇ཰ࡉࢀࡓ  㢌ࡢ࠺ࡕ MAT ࡛  㢌㸦㸧ࡀᢠయ㝧ᛶࢆ♧ࡋࡓࠋ
࣭PCR ࡛ࡣ  㢌୰  㢌㸦㸧࡛ࣞࣉࢺࢫࣆࣛ㑇ఏᏊࡀ᳨ฟࡉࢀࡓࠋࡇࢀࡽࡢ⤖ᯝ࠿ࡽරᗜ┴ࡢ࢔ࣛ࢖ࢢ࣐࡟ࡣᗈࡃࣞࣉࢺࢫࣆࣛࡀឤᰁࡋ࡚࠸ࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗ ࡓࠋ࣭࢔ࣛ࢖ࢢ࣐ࡢ⏕ᜥᆅᇦࡢᣑ኱࡟ࡼࡾࠊேࡢ⏕ά⎔ቃ࡬ࡢ᥋ゐࡢᶵ఍ࡀቑࡍࡇ࡜࡛࢔ࣛ࢖ࢢ࣐࠿ࡽே࡬ࡢឤᰁࡢࣜࢫࢡࡀ㧗ࡲࡿྍ⬟ᛶࡀ⪃࠼ࡽࢀࡿࠋ㸬ࡣࡌࡵ࡟ࣞࣉࢺࢫࣆࣛࡣࢫࣆ࣮ࣟ࣊ࢱ┠ࣞࣉࢺࢫࣆࣛ⛉࡟ศ㢮ࡉࢀࡿࢢ࣒ࣛ㝜ᛶዲẼᛶࡽࡏࢇ⳦࡛࠶ࡾࠊேࡸ≟ࢆࡣࡌࡵ࡜ࡍࡿከࡃࡢ့ஙື≀࡟ឤᰁࡋࠊ⾑Ⰽ⣲ᒀࠊᛴᛶ⭈୙඲ࠊ㯤⑇࡞࡝ࢆ࿊ࡍࡿே␆ඹ㏻ឤᰁ⑕ࡢ⑓ཎ⳦࡜ࡋ࡚㔜せ࡛࠶ࡿ㹙㹛ࠋேࣞࣉࢺࢫࣆࣛ⑕ࡣឤᰁ⑕ἲᅄ㢮ឤᰁ⑕࡟ᣦᐃࡉࢀࡿᛴᛶ⇕ᛶ⑌ᝈ࡛࠶ࡾࠊ㯤⑇ࠊฟ⾑ࠊ⭈㞀ᐖࢆక࠺㔜⑕ᆺ࠿ࡽࠊឤෑᵝ⑕≧ࡢࡳࡢ㍍⑕ᆺࡲ࡛ᵝࠎ࡞⑕≧ࢆ♧ࡍ㹙㹛ࠋࡲࡓࠊ≟ࣞࣉࢺࢫࣆࣛ⑕ࡣᐙ␆ఏᰁ⑓ண㜵ἲ࡟࠾ࡅࡿᒆฟఏᰁ⑓࡛࠶ࡾࠊᛴᛶࡢ⭈୙඲ࠊ⫢୙඲ࠊ⾑ᾮจᅛ୙඲ࢆ࿊ࡋ᪩ᮇ࡟Ṛஸࡍࡿ౛ࡶከ࠸㹙㹛ࠋ⎔ቃ୰࡟࠾ࡅࡿࣞࣉࢺࢫࣆࣛࡢಖ⳦ື≀ࡣࡆࡗṑ㢮ࢆࡣࡌࡵ࡜ࡋࡓ㔝⏕ື≀࡛࠶ࡾࠊឤᰁࡋࡓື≀ࡢከࡃࡀࣞࣉࢺࢫࣆࣛࢆ⭈⮚࡟ಖ⳦ࡋࠊᒀ୰࡟᤼ἥࡍࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿࠋேࠊᐙ␆ࠊక౶ື≀࡛࠶ࡿ≟ࡣࡇࢀࡽಖ⳦ື≀ࡢᒀ࡜┤᥋ࠊࡲࡓࡣࣞࣉࢺࢫࣆࣛࢆྵࡴᒀ࡛ởᰁࡉࢀࡓỈࡸᅵተ࡞࡝࡜ࡢ㛫᥋ⓗ࡞᥋ゐ࡟ࡼࡾឤᰁࡍࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿ㹙㹛ࠋʵɹɹʵ㏆ᖺࠊಶయᩘࡀᛴ⃭࡟ቑຍࡋࠊேࠊᐙ␆ࠊ≟࡞࡝ࡢ⏕άᅪ࡟ฟἐࡍࡿᶵ఍ࡢቑ࠼࡚࠸
ࡿ࢔ࣛ࢖ࢢ࣐㸦Procyon lotor㸧ࡶࡲࡓࠊࣞࣉࢺࢫࣆࣛࡢಖ⳦ື≀࡜࡞ࡿྍ⬟ᛶࡢ࠶ࡿື≀ࡢ  ࡘ࡛࠶ࡿࠋᾏእ࡛ࡣࠊࡇࢀࡲ࡛࡟࢔ࣛ࢖ࢢ࣐࡟࠾ࡅࡿ㢧ᚤ㙾ୗจ㞟ヨ㦂
㸦microscopic agglutination test, MAT㸧ࢆ⏝࠸ࡓࣞࣉࢺࢫࣆࣛᢠయಖ᭷≧ἣㄪᰝࢆࡣࡌࡵࠊ⭈⮚ࡢ⑓⌮Ꮫⓗ᳨ᰝࠊ⭈⮚࠾ࡼࡧᒀ࠿ࡽ⳦యࡢศ㞳࡞࡝ከࡃࡢሗ࿌ࡀ࠶ࡾ㹙㹛ࠊ࢔ࣛ࢖ࢢ࣐ࡀࣞࣉࢺࢫࣆࣛࡢಖ⳦ື≀࡜࡞ࡗ࡚࠸ࡿྍ⬟ᛶࡀ♧၀ࡉࢀ࡚࠸ࡿࠋࡲࡓࠊ㔝⏕࢔ࣛ࢖ࢢ࣐ᤕ⋓⪅࡟࠾ࡅࡿࣞࣉࢺࢫࣆࣛ⑕ࡢⓎ⑕஦౛ࡢሗ࿌ࡀ࠶ࡾ㹙㹛ࠊ㔝⏕ࡢ࢔ࣛ࢖ࢢ࣐ࡀேࡢࣞࣉࢺࢫࣆࣛ⑕ࡢឤᰁ※࡜࡞ࡿྍ⬟ᛶࡶᙉࡃ♧၀ࡉࢀ࡚࠸ࡿࠋ୍᪉ࠊ᪥ᮏ࡟࠾ࡅࡿ࢔ࣛ࢖ࢢ࣐ࡢࣞࣉࢺࢫࣆࣛ࡟㛵ࡍࡿㄪᰝ࡟ࡣࠊᡃࠎࡀㄪ࡭ࡓ㝈ࡾ࡛ࡣࠊձ ᖺ  ᭶㹼 ᭶࡟ᐇ᪋ࡉࢀࡓ໭ᾏ㐨ෆ㔝⏕࢔ࣛ࢖ࢢ࣐ࡢㄪᰝࠊղ ᖺ ᭶㹼 ᖺ  ᭶࡟ᐇ᪋ࡉࢀࡓ⚄ዉᕝ┴ෆ㔝⏕࢔ࣛ࢖ࢢ࣐࠾ࡼࡧ㛗ᓮ┴ෆື≀ᒎ♧᪋タ࢔ࣛ࢖ࢢ࣐ࡢㄪᰝࡢ㸰ࡘࡢᏛ఍ሗ࿌ࠊճ ᖺ  ᭶㹼 ᖺ  ᭶࡟ᐇ᪋ࡉࢀࡓ኱㜰ᗓࡢື≀⏤᮶ឤᰁ⑕ࢧ࣮࣋࢖ࣛࣥࢫࡢ୍㒊࡜ࡋ࡚ࡢሗ࿌ࡀ࠶ࡿࡢࡳ࡛࠶ࡿࠋࡑࡇ࡛ᮏ◊✲࡛ࡣࠊ㔝⏕࢔ࣛ࢖ࢢ࣐ࡢࣞࣉࢺࢫࣆࣛឤᰁ≧ἣࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࢆ┠ⓗ࡜ࡋ࡚ࠊරᗜ┴ࡢ࢔ࣛ࢖ࢢ࣐࡟࠾࠸࡚ࠊMAT ࢆ⏝࠸࡚ࣞࣉࢺࢫࣆࣛᢠయಖ᭷⋡ࡢㄪᰝࢆ⾜ࡗࡓࠋࡉࡽ࡟ࠊࣞࣉࢺࢫࣆࣛእ⭷⺮ⓑ OmpL1 㑇ఏᏊࢆᶆⓗ࡜ࡋࡓ PCR
㸦OmpL1-PCR㸧ࢆ⏝࠸ࡓㄪᰝࡶྠ᫬࡟⾜ࡗࡓࠋ㸬ᮦᩱ࡜᪉ἲ㸦㸯㸧ㄪᰝᆅᇦ࠾ࡼࡧ⿕㦂᳨య࢔ࣛ࢖ࢢ࣐ࡢ᳨యࡣ᭷ᐖᤕ⋓ಶయ࠾ࡼࡧ஺㏻஦ᨾ࡛Ṛஸࡋࡓಶయ࠿ࡽ᥇ྲྀࡉࢀࡓ㸦❶ཧ↷㸧ࠋ࢔ࣛ࢖ࢢ࣐ࡢ᳨యࡣࠊ ᖺ  ᭶㹼 ᖺ  ᭶࡟ᤕ⋓ࡉࢀࡓ࢔ࣛ࢖ࢢ࣐࠿ࡽ⾑Ύ  ᳨యࠊ⭈⮚  ᳨యࢆ᥇ྲྀࡋࠊ⾑Ύ᳨యࡣΥࠊ⭈⮚᳨యࡣΥࡢࣇ࣮ࣜࢨ࣮ෆ᳨࡛ᰝ࡟⏝࠸ࡿࡲ࡛෭෾ಖᏑࡋࡓࠋ᳨ᰝ࡟౪ࡋࡓ࢔ࣛ࢖ࢢ࣐ࡣࠊ㌟యⓗ࡟≉࡟␗ᖖࢆㄆࡵࡓࡶࡢࡣ࡞ࡃࠊ೺ᖖ࡞࢔ࣛ࢖ࢢ࣐࡛࠶ࡗࡓࠋ
㸦㸰㸧ࣞࣉࢺࢫࣆࣛ⳦ᰴ
MAT ࡢᢠཎ࡜ࡋ࡚ࡣᶆ‽ᰴ࡜ࡋ࡚ᇵ㣴ࠊ⥔ᣢࡉࢀ࡚࠸ࡿ L. interrogans serovar
icterohaemorrhagiae㸦strain RGA㸧ࠊL. interrogans serovar canicola㸦strain Hond
Utrechtϫ㸧ࠊL. interrogans serovar autumnalis㸦strain Akiyami A㸧ࠊL. interrogans
serovar hebdomadis㸦strain Akiyami B㸧ࠊL. interrogans serovar australis㸦strainAkiyami C㸧ࢆ⏝࠸ࡓࠋࣞࣉࢺࢫࣆࣛࡢᇵ㣴࡟ࡣࠊWHO Human Leptospirosis㸸
Guidance for Diagnosis, Surveillance and Control 㹙㹛࡟ᇶ࡙࠸࡚స〇ࡉࢀࡓEMJH ᇵᆅࢆ⏝࠸ࠊ㹼Υ࡛ᇵ㣴ࡋࡓࠋʵɹɹʵ

㸦㸱㸧MAT
MAT ࡣ WHO Human Leptospirosis: Guidance for Diagnosis, Surveillance andControl ࡟ᇶ࡙࠸࡚⾜ࡗࡓ㹙㹛ࠋࡍ࡞ࢃࡕࠊ๓㏙ࡢ  ✀㢮ࡢࣞࣉࢺࢫࣆࣛᶆ‽ᰴࢆ౪ヨᢠཎ࡜ࡋ࡚ࠊ⳦ᩘࡀ  ಶPO ࡟࡞ࡿࡼ࠺࡟ࣜࣥ㓟⦆⾪ᾮ㸦PBS㸧࡛ㄪ〇ࡋࡓࠋ⿕㦂⾑Ύࡣ PBS ࡛  ಸᕼ㔘ࡋࡓࡢࡕࠊ96 ✰࣐࢖ࢡࣟࣉ࣮ࣞࢺ㸦Thermo Fisher Scientific,Waltham, MA㸧ୖ࡛  ಸࡲ࡛ PBS ࢆ⏝࠸࡚ẁ㝵ᕼ㔘ࡋࡓࠋᕼ㔘⾑Ύ࡜ྠ㔞ࡢ౪ヨᢠ
ཎࢆྛ ZHOO ࡟ศὀࡋࠊ᭱⤊ᐜ㔞ࢆ ȣOࠊ᭱⤊ᕼ㔘⋡ࢆࡑࢀࡒࢀ ࠊࠊࠊ ಸ࡜ࡋࡓࠋࡑࡢᚋ Υ࡛  ᫬㛫཯ᛂࡉࡏࡓᚋࠊᬯど㔝㢧ᚤ㙾ୗ࡛ほᐹࡋࠊจ㞟ࡋ࡚࠸࡞࠸㐟㞳⳦ᩘࡀ⾑Ύࢆྵࡲ࡞࠸ᑐ↷࡜ẚ㍑ࡋ࡚ 㸣௨ୗ࡜࡞ࡿ᭱⤊ᕼ㔘ಸ⋡ࢆจ㞟ᢠయ౯࡜ࡋࡓࠋᢠయ౯ࡀ  ಸ௨ୖࡢࡶࡢࢆ㝧ᛶ࡜ࡋࠊ㸯⾑Ύᆺ௨ୖ࡛㝧ᛶࡢࡶࡢࢆ 0$7 㝧ᛶ࡜ࡋࡓࠋࡉࡽ࡟ࠊจ㞟ᢠయ౯ࡀ  ಸ௨ୖ࡛࠶ࡗࡓࡶࡢ࡟㛵ࡋ࡚ࡣࠊࡉࡽ࡟⾑Ύࡢẁ㝵ᕼ㔘ࢆ㐍ࡵࠊ᭱⤊ᕼ㔘ಸ⋡ࢆồࡵࠊจ㞟ᢠయ౯࡜ࡋࡓࠋ
㸦㸲㸧3&5 ࢆ⏝࠸ࡓ࢔ࣛ࢖ࢢ࣐⭈⓶㉁࡟࠾ࡅࡿࣞࣉࢺࢫࣆࣛ㑇ఏᏊࡢ᳨ฟ࢔ࣛ࢖ࢢ࣐ࡢ⭈⮚  ᳨య࡟࠾࠸࡚ࠊ⭈⓶㉁㡿ᇦࡢ୍㒊㸦PJ ⛬ᗘ㸧ࢆ᥇ᮦࠊ࣍ࣔࢪ
࢙ࢼ࢖ࢬࡋࠊQIAamp DNA Mini Kit㸦QIAGEN GmbH, Hilden, Germany㸧ࢆ⏝࠸࡚DNA ࡢᢳฟࢆ⾜࠸ࠊȣO ࡜ࡋࡓࠋᢳฟࡋࡓࢧࣥࣉࣝ DNA ࡣ౑⏝ࡍࡿࡲ࡛Υࡢࣇ࣮ࣜࢨ࣮ෆ࡟࡚ಖᏑࡋࡓࠋࡑࢀࡒࢀࡢ⭈⓶㉁࠿ࡽᢳฟࡋࡓࢧࣥࣉࣝ DNA ȣO ࢆ㗪ᆺ࡜
ࡋࠊL. kirschneri serovar Grippotyphosa ࡢ OmpL1 㑇ఏᏊ㓄ิ㹙㹛ࢆᇶ࡟స〇ࡉࢀࡓ ࣉ ࣛ ࢖ ࣐ ࣮ OmpL1-Pr.1 㸦 GCCGTAGCATTATCTTCG 㸧ࠊ OmpL1-Pr.2
㸦ACCCCAGGTCATATCTAC㸧ࢆ⏝࠸࡚ PCR ࢆ⾜࠸ࠊࣞࣉࢺࢫࣆࣛ㑇ఏᏊࡢ᳨ฟࢆ⾜ࡗࡓࠋPCR ࡛ࡣࠊ⇕ኚᛶࢆ Υ ศࠊ࢔ࢽ࣮ࣜࣥࢢࢆ Υ ศࠊఙ㛗཯ᛂ Υศ  ⛊ࢆ  ࢧ࢖ࢡࣝ࡜ࡋࠊィ  ࢧ࢖ࢡࣝ⾜࠸ࠊ᭱ᚋࡢఙ㛗཯ᛂࡣ Υ࡛  ศ㛫⾜ࡗࡓࠋOmpL1-PCR ࡟ࡼࡗ࡚ቑᖜࡉࢀࡓ DNA ࡣ 㸣ࡢ࢔࣮࢞ࣟࢫࢤࣝࢆ⏝࠸࡚㟁ẼὋືࢆ⾜࠸ࠊ࢚ࢳࢪ࣒࢘ࣈ࣐ࣟ࢖ࢻ࡛ᰁⰍᚋࠊ⣸እ⥺ୗ࡛ࣂࣥࢻࢆ☜ㄆࡋࡓࠋOmpL1-PCR࡟࠾࠸࡚ࣂࣥࢻࡀ☜ㄆࡉࢀࡓࢧࣥࣉࣝ࡟㛵ࡋ࡚ࠊࢩ࣮ࢡ࢚ࣥࢫ཯ᛂࢆ⾜࠸ࠊᒣཱྀ኱Ꮫ㑇ఏᏊᐇ㦂᪋タ࡟ሷᇶ㓄ิゎᯒࢆ౫㢗ࡋࡓࠋ㸬⤖ᯝ࡜⪃ᐹ㸦㸯㸧MAT ࢆ⏝࠸ࡓᢠయ౯
᳨⣴ࡋࡓ࢔ࣛ࢖ࢢ࣐  㢌୰  㢌㸦㸣㸧ࡀ࠸ࡎࢀ࠿ࡢ⾑Ύᆺ࡛ᢠయ㝧ᛶࢆ♧ࡋࡓ ࠋ ྛ ⾑ Ύ ᆺ 㝧 ᛶ 㢌 ᩘ ࡣ ࠊ hebdomadis  㢌 㸦  㸣 㸧 ࡀ ᭱ ࡶ ከ ࡃ ࠊ ḟ ࠸ ࡛icterohaemorrhagiae  㢌㸦㸣㸧ࠊaustralis  㢌㸦㸣㸧ࠊautumnalis  㢌㸦㸣㸧ࠊ
canicola  㢌㸦㸣㸧࡛࠶ࡗࡓ㸦⾲ 㸧ࠋ

ʵɹɹʵ


Icterohaemorrhagiae

10Canicola
Autumnalis
Hebdomadis
Australis

MATMAT

hebdomadis

MATʵɹɹʵᆅ᪉㸧࡛ᤕ⋓ࡉࢀࡓ㔝⏕ࡢ࢔ࣛ࢖ࢢ࣐  㢌࡟࠾࠸࡚ᐇ᪋ࡉࢀࡓ  ✀ࡢ⾑Ύᆺࢆ⏝࠸
ࡓ MAT ࡢ⤖ᯝ࡛ࡣࠊ 㢌㸦㸣㸧࡛࠸ࡎࢀ࠿ࡢ⾑Ύᆺ࡟㝧ᛶࡀㄆࡵࡽࢀ࡚࠾ࡾࠊ
autumnalisࠊicterohaemorrhagiaeࠊcanicola ࡟㝧ᛶࢆ♧ࡍ౛ࡀከࡃㄆࡵࡽࢀ࡚࠸ࡿࠋࡲࡓࠊ ᖺ  ᭶㹼 ᖺ  ᭶⚄ዉᕝ┴ෆ࡛ᤕ⋓ࡉࢀࡓ㔝⏕࢔ࣛ࢖ࢢ࣐  㢌࠾ࡼࡧ㛗ᓮ┴ෆࡢື≀ᒎ♧᪋タ࡛㣫⫱ࡉࢀ࡚࠸ࡓ࢔ࣛ࢖ࢢ࣐  㢌࡟࠾࠸࡚ࠊ᳨ᰝἲࡣ୙࡛᫂
࠶ࡿࡀ㔝⏕ࡢ࢔ࣛ࢖ࢢ࣐  㢌㸦㸣㸧ࠊື≀ᒎ♧᪋タࡢ࢔ࣛ࢖ࢢ࣐  㢌㸦㸣㸧࡛ᢠయ౯ࡢୖ᪼ࡀㄆࡵࡽࢀ࡚࠸ࡿࠋᮏ◊✲࡟࠾ࡅࡿ㔝⏕ࡢ࢔ࣛ࢖ࢢ࣐ࡢ 0$7 ࡢ⤖ᯝࡣࠊ㐣ཤࡢ௚ࡢᆅᇦ࡟࠾ࡅࡿ࢔ࣛ࢖ࢢ࣐ࡢᏛ఍ሗ࿌࡜ẚ㍑ࡋ࡚㧗࠸㝧ᛶ⋡ࢆ♧ࡋࡓࡀࠊࡇࢀࡣᮏ◊✲࡟࠾ࡅࡿㄪᰝᆅᇦ࡟ࡼࡿᆅᇦᛶࢆ཯ᫎࡋ࡚࠸ࡿ࡜⪃࠼ࡽࢀࡓࠋࡲࡓࠊᡃࡀᅜࡢ⌧⾜ࡢ≟ࣞࣉࢺࢫࣆࣛ୙ά໬࣡ࢡࢳࣥ࡟ྵࡲࢀ࡚࠸ࡿ⾑Ύᆺࡣ icterohaemorrhagiaeࠊ
canicola ࡢ  ✀ΰྜࡀ୺࡛࠶ࡾࠊ ♫ࡢ〇ရࡢࡳࡀ copenhageni
㸦 icterohaemorrhagiae࡜ྠ୍⾑Ύ⩌࡟ᒓࡍࡿ㏆⦕ࡢ⾑Ύᆺ㸧ࠊcanicolaࠊ hebdomadis ࡢ  ✀ΰྜ࡛࠶ࡿࠋ௒
ᅇࠊරᗜ┴ࡢ࢔ࣛ࢖ࢢ࣐࡟࠾࠸࡚ hebdomadis ࡟ᑐࡍࡿᢠయ㝧ᛶ⋡ࡀⴭࡋࡃ㧗࠿ࡗࡓࡇ࡜ࡣࠊྠᆅᇦ࡛㣫⫱ࡉࢀ࡚࠸ࡿ≟࡟࣡ࢡࢳࣥࢆ᥋✀ࡍࡿ㝿ࡢ࣡ࢡࢳࣥ㑅ᢥ࡟㔜せ࡞▱ぢ࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿࠋ

㸦㸰㸧PCR ࢔ ࣛ ࢖ ࢢ ࣐ ࡢ ⭈ ⓶ ㉁ ࡟ ࠾ ࠸ ࡚ ࠊ OmpL1 㑇 ఏ Ꮚ ࢆ ࢱ ࣮ ࢤ ࢵ ࢺ ࡜ ࡋ ࡓ PCR
㸦 OmpL1-PCR㸧ࢆ⾜ࡗࡓ⤖ᯝࠊ 㢌୰  㢌㸦㸣㸧࡛㝧ᛶࡀㄆࡵࡽࢀࡓࠋḟ࡟

OmpL1-PCR 㝧ᛶ࡛࠶ࡗࡓ  㢌ࡢ PCR ⏘≀ࡢሷᇶ㓄ิࢆゎᯒࡋࡓ࡜ࡇࢁࠊ 㢌࡛ゎᯒࡀྍ⬟࡛࠶ࡗࡓࠋࡇࢀࡽࡢ㓄ิࡣ࠸ࡎࢀࡶྠ୍࡛࠶ࡾࠊ ⾑Ύᆺࡢᶆ‽ᰴࡢ OmpL1 㑇ఏ Ꮚ ࡜ ࡢ ሷ ᇶ 㓄 ิ ࡢ ┦ ྠ ᛶ ࢆ ẚ ㍑ ࡋ ࡓ ࡜ ࡇ ࢁ ࠊ hebdomadis ࡜  㸣 ࠊicterohaemorrhagiae ࡜ ࡢ┦ྠᛶࢆ♧ࡋࡓ㸦⾲ 㸧ࠋ⾲ 㻞㻌 䜰䝷䜲䜾䝬⭈⓶㉁䜘䜚᳨ฟ䛥䜜䛯 㻻㼙㼜㻸㻝 㑇ఏᏊ 㻠㻠㻝㼎㼜 䛸ᶆ‽ᰴሷᇶ㓄ิ䛾ẚ㍑䠄㻑䠅㻌㻌㻌䜰 䝷 䜲䜾䝬㻌㻵㼏㼠㼑㼞㼛㼔㼍㼑㼙㼛㼞㼞㼔㼍㼓㼕㼍㼑㻌 㻯㼍㼚㼕㼏㼛㼘㼍㻌 㻭㼡㼠㼡㼙㼚㼍㼘㼕㼟㻴㼑㼎㼐㼛㼙㼍㼐㼕㼟㻭㼡㼟㼠㼞㼍㼘㼕㼟㻴㼍㼞㼐㼖㼛㻌㻥㻥㻚㻜㻥㻌 㻌㻥㻥㻚㻡㻡㻌 㻌㻥㻤㻚㻢㻠㻌㻥㻝㻚㻝㻢㻌 㻥㻝㻚㻝㻢㻌 㻌 㻥㻜㻚㻥㻟㻌 㻌㻥㻜㻚㻠㻤㻌 㻌 㻥㻤㻚㻢㻠㻌 㻌㻼㼛㼙㼛㼚㼍㻌 㻼㼥㼞㼛㼓㼑㼚㼑㼟  ᖺ  ᭶㹼 ᖺ  ᭶ࡢ኱㜰ᗓື≀⏤᮶ឤᰁ⑕ࢧ࣮࣋࢖ࣛࣥࢫࡢሗ࿌࡟࠾࠸࡚ࠊflaB 㑇ఏᏊࢆᶆⓗ࡜ࡋࡓ PCR ࢆ⏝࠸࡚࢔ࣛ࢖ࢢ࣐ࡢᒀࢆㄪᰝࡋࡓ⤖ᯝࠊ 㢌୰ 㢌㸦㸣㸧࡛㝧ᛶࢆㄆࡵ࡚࠸ࡿࠋࡇࡢ⤖ᯝࡣ࢔ࣛ࢖ࢢ࣐ࡀᒀ୰࡟ࣞࣉࢺࢫࣆࣛࢆ᤼ฟࡋ࡚࠾ࡾࠊឤᰁ※࡜࡞ࡿྍ⬟ᛶࢆ♧၀ࡋ࡚࠸ࡿ࡜⪃࠼ࡽࢀࡿࠋࣞࣉࢺࢫࣆࣛࡣឤᰁᚋࠊឤᰁື≀ࡢ⭈⮚ࡢ⭈ᒀ⣽⟶࡟࠾࠸࡚ᐃ╔࣭ቑṪࡋࠊ㛫㉁ᛶ⭈⅖ࢆᘬࡁ㉳ࡇࡍ࡜࠸ࢃࢀ࡚
࠸ࡿ>㸪@ࠋᾏእࡢㄪᰝ࡟࠾࠸࡚ࠊ㔝⏕ࡢ࢔ࣛ࢖ࢢ࣐  㢌ࡢ⭈⮚ࢆㄪᰝࡋࡓ⤖ᯝࠊ 㢌࡛⫗║ⓗ࡞␗ᖖࢆㄆࡵࠊ 㢌࡛⑓⌮⤌⧊Ꮫⓗ᳨ᰝ࡟࠾࠸࡚㛫㉁ᛶ⭈⅖ࡀㄆࡵࡽࢀ࡚࠸ࡿࠋࡉࡽ࡟ࠊ㛫㉁ᛶ⭈⅖ࡀㄆࡵࡽࢀࡓ࢔ࣛ࢖ࢢ࣐ࡢ࠺ࡕࠊ 㢌࡛⳦యࡀ᳨ฟ

ʵɹɹʵ


㸦Warthin-Starry Silver stain㸧ࡉࢀࠊ⳦యࡢศᕸࡣ࠸ࡎࢀࡶ⭈⓶㉁ࡢ⭈ᒀ⣽⟶࡟㝈ᒁࡋ࡚࠸ࡓ㹙㹛ࠋᮏ◊✲࡛ࡣࠊOmpL1 㑇ఏᏊࢆࢱ࣮ࢤࢵࢺ࡜ࡋࡓ PCR ࢆ⏝࠸࡚࢔ࣛ࢖ࢢ࣐ࡢ⭈⮚᳨యࡢ⓶㉁㡿ᇦ࠿ࡽࣞࣉࢺࢫࣆࣛ㑇ఏᏊࡢ᳨ฟࢆ⾜ࡗࡓࠋ௒ᅇㄪᰝࡋࡓ⭈⮚࡟࠾࠸࡚ࠊ⑓⌮⤌⧊Ꮫⓗ᳨⣴ࡣ⾜ࡗ࡚ࡣ࠸࡞࠸ࡀࠊᑡ࡞ࡃ࡜ࡶ⫗║ⓗ࡟␗ᖖࡣㄆࡵࡽࢀ
࡞࠿ࡗࡓࠋࢹ࣮ࢱ࡟ࡣ♧ࡋ࡚࠸࡞࠸ࡀ OmpL1-PCR ࡜ࡢẚ㍑࡜ࡋ࡚ࠊࣞࣉࢺࢫࣆࣛ㑇ఏᏊ᳨ᰝ࡟ᗈࡃ⏝࠸ࡽࢀ࡚࠸ࡿ flaB-PCR ࢆྠᵝ࡟⾜ࡗࡓ࡜ࡇࢁࠊOmpL1-PCR ࡜ẚ㍑ࡋ࡚ឤᗘࡀప࠸⤖ᯝ࡜࡞ࡗࡓࠋࡲࡓࠊணഛᐇ㦂࡟࠾࠸࡚ࠊࣞࣉࢺࢫࣆࣛᇵ㣴ᾮ㸦 L.

interrogans serovar icterohaemorrhagiae 㸧 ࠿ ࡽ ᚓ ࡽ ࢀ ࡓ DNA ࡢ ᕼ 㔘 ࢆ ⾜ ࠸ ࠊOmpL1-PCR ࠾ࡼࡧ flaB-PCR ࡢ᳨ฟឤᗘࢆẚ㍑ࡋࡓ࡜ࡇࢁࠊࡑࢀࡽࡢឤᗘࡣ࡯ࡰྠ➼࡛࠶ࡗࡓࡇ࡜࠿ࡽ㸦ᮍⓎ⾲ࢹ࣮ࢱ㸧ࠊᮏ◊✲࡛⏝࠸ࡓ OmpL1-PCR ࡣ flaB-PCR ࡜ྠ➼࠶ࡿ࠸ࡣࡑࢀ௨ୖࡢឤᗘࢆ᭷ࡍࡿ࡜⪃࠼ࡽࢀࡓࠋ㸬⤖ㄽᮏ◊✲࡟࠾࠸࡚ࠊරᗜ┴ࡢ࢔ࣛ࢖ࢢ࣐ࡣ㧗࠸☜⋡࡛⾑Ύ୰࡟ࣞࣉࢺࢫࣆࣛᢠయࢆಖ᭷ࡋ࡚࠸ࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋࡲࡓࠊ࢔ࣛ࢖ࢢ࣐ࡢ⭈⮚࠿ࡽࣞࣉࢺࢫࣆࣛ㑇ఏᏊࡀ᳨ฟࡉࢀࡓࡇ࡜࠿ࡽࠊ࢔ࣛ࢖ࢢ࣐ࡣ⭈⮚࡟ࣞࣉࢺࢫࣆࣛࢆಖ᭷ࡋ࡚࠸ࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡾࠊᒀ୰࡬᤼ἥࡋ࡚࠸ࡿྍ⬟ᛶࡀᙉࡃ♧၀ࡉࢀࡓࠋ௨ୖࡢࡇ࡜࠿ࡽࠊ㔝⏕ࡢ࢔ࣛ࢖ࢢ࣐ࡣࣞࣉࢺࢫࣆࣛࡢಖ⳦ື≀࡜࡞ࡗ࡚࠾ࡾࠊே࡬ࡢឤᰁ※࡜࡞ࡿ༴㝤ᛶࡀ⪃࠼ࡽࢀࡿࠋࡉࡽ࡟࢔ࣛ࢖ࢢ࣐ࡣᮏ᮶ࠊ໭⡿ࢆཎ⏘࡜ࡍࡿእ᮶⏕≀࡛࠶ࡾࠊᮏ◊✲࡛ࡣㄪᰝࡋ࡚࠸࡞࠸௒ࡲ࡛᪥ᮏ࡟࠾࠸࡚Ꮡᅾࡋ࡞࠿ࡗࡓ⾑Ύᆺࡢࣞࣉࢺࢫࣆࣛࢆಖ᭷ࡋ࡚࠸ࡿྍ⬟ᛶࡶྰᐃ࡛ࡁ࡞࠸ࠋ࢔ࣛ࢖ࢢ࣐࡞࡝ࡢ㔝⏕ື≀࡟࠾ࡅࡿࣞࣉࢺࢫࣆࣛᢠయಖ᭷≧ἣㄪᰝࡣேࢆྵࡵࡓ௚ࡢື≀✀࡟࠾ࡅࡿࣞࣉࢺࢫࣆࣛឤᰁ⑕ࡢ༴㝤ண⟶⌮࡟࠾࠸࡚ࡶࠊ⋇་⮫ᗋᏛୖࠊබ⾗⾨⏕Ꮫୖ㔜せ࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿࠋࡲࡓࠊ࢔ࣛ࢖ࢢ࣐ࢆᤕ⋓ࡋࠊಶయᩘㄪᩚࢆ⾜࠺ࡇ࡜࡛ࠊࣞࣉࢺࢫࣆ
ࣛࢆࡣࡌࡵ࡜ࡍࡿே␆ඹ㏻ឤᰁ⑕ࡢᗈࡀࡾࢆ㜵ࡄ࡜࡜ࡶ࡟ࠊࡇࢀࡽࡢㄪᰝࢆ⾜࠺ࡇ࡜࡛ே
ࡸ௚ࡢື≀✀࡬ࡢឤᰁࢆ㜵ࡄࡇ࡜ࡀྍ⬟࡟࡞ࡿ࡜⪃࠼ࡽࢀࡿࠋࣞࣉࢺࢫࣆࣛ࡟㛵ࡋ࡚ࡣ௒ᚋࠊ㔝እ࠿ࡽࡢ⳦ศ㞳ࢆྵࡵࡓࡉࡽ࡞ࡿㄪᰝࢆ㐍ࡵ࡚࠸ࡃࡇ࡜ࡀ⫢せ࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿࠋ

ᘬ⏝ᩥ⊩

1.

⳥ụ┤ဢ㸦1999㸧⋇་ឤᰁ⑕࣮࢝ࣛ࢔ࢺࣛࢫࠊ➨㸯∧: 283-286. 㸦ぢୖ᙭ࠊ୸ᒣົ┘ಟ㸧ࠊᩥỌᇽฟ∧ࠊᮾி.

2.

World Health Organization(WHO) 㸦2003㸧Human Leptospirosis: Guidance forʵɹɹʵ

Diagnosis, Surveillance and control㸪WHO㸪Geneva.
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Faine S㸪Adler B㸪Bolin C㸪Perolat P㸦1999㸧Leptospira and Leptospirosis㸪
2nd ed㸪MediSci㸪Melbourne.

4.

Levett PN㸸Leptospirosis㸪Clin Microbiol 㸦2001㸧Rev. 14: 296-326.

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Katrin H㸪Craig EG㸦2005㸧Disease caused by systemic bacterial infection.
In:Textbook of Veterinary Internal Medicine 㸪 pp.616-619. Ettinger SJ 㸪
Feldoman EC㸪6th eds㸪Elsevier Saunders,St.Louis.

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Mitchell MA㸪Hungeford LL㸪Nixon C㸪Esker T㸪Sullivan J㸪Koerkenmeier R㸪
Dubey JP㸦1999㸧Serologic survey for selected infectious disease agents inraccoons from Illinois. J. Wildl. Dis. 35: 347-55.

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Richardson DJ㸪Gauthier JL.㸦2003㸧A serosurvey of leptospirosis in Connecticut
peridomestic wildlife㸪Vector Borne Zoonotic Dis. 3: 187-93.

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Junge RE㸪Bauman K㸪King M㸪Gompper ME.㸦2007㸧A serologic assessmentof exposure to viral pathogens and Leptospira in an urban raccoon (Procyonlotor) population inhabiting a large zoological park. J. Zoo. Wildl. Med. 38:18-26.

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Davis MA㸪Evermann JF㸪Petersen CR㸪VancerSchalie J㸪Besser TE㸪Huckabee
J㸪Daniels JB㸪Hancock DD㸪Leslie M㸪Baer R㸦2008㸧Serological survey forantibodies to Leptospira in dogs and raccoons in Washington State. Zoonoses
Public Health. 55: 436-42.

10. Hamir AN㸪Hanlon CA㸪Niezgoda M㸪Rupprecht CE㸦2001㸧The prevalence ofinterstitial nephritis and leptospirosis in 283 raccoons (procyon lotor) from 5
different sites in the United States㸪Can. Vet. J. 42: 869-71.
11. Warshawsky B㸪Lindsay LR㸪Artsob H㸦2000㸧Leptospira infections in trappers
from Ontario㸪Can. J. Infect. Dis.11: 47-51.
12. Haake DA㸪Champion CI㸪Martinich C㸪Shang ES㸪Blanco DR㸪Miller JN㸪
Lovett MA㸦1993㸧Molecular cloning and sequence analysis of the gene encoding
OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira
spp㸪J. Bacteriol. 175: 4225-34.
13. Yang CW䠈 Wu MS䠈Pan MJ㻌 䠄2001䠅Leptospirosis renal disease䠈Nephrol DialTransplant䠈16: 73-77.

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BOX2ʵɹɹʵ

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