Quick GuideZEISS TWAIN for Axiocam 202/208

ZEISS TWAIN for Axiocam 202/208
Original Manual
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 1007745 Jena
Germany
microscopy@zeiss.com
www.zeiss.com/microscopy

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 1007745 Jena
Germany

Document Name: ZEISS TWAIN for Axiocam 202/208 Quick Guide
Revision: 1
Language: en-US
Effective from: 06/2020© 2020 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced ortransmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any information or filing system. The right to make backup-copies for archiving purposes shall remain unaffected thereby. Any violations may be prosecuted as copyright infringements.
The use of general descriptive names, registered names, trademarks, etc. in this document does not imply that such names are
exempt from the relevant intellectual property laws and regulations and therefore free for general use. This shall also apply if
this is not specifically referred to. Software programs shall entirely remain the property of ZEISS. No program or subsequent upgrade thereof may be disclosed to any third party, copied or reproduced in any other form without the prior written consent ofZEISS, even if these copies or reproductions are destined for internal use at the customer's only, the only exception being one
single back-up copy for archiving purposes.
ZEISS reserves the right to make modifications to this document without notice.

​ Table of Contents​

ZEISS

Table of Contents
1 General Information ........................................................................ 41.1

Intended Use..............................................................................................

41.2

Contact ......................................................................................................

42 Operation Requirements.................................................................. 52.1

Hardware ...................................................................................................

52.2

Software ....................................................................................................

52.3

Camera Connection Scheme.......................................................................

63 Software Description....................................................................... 7
3.1

General User Interface................................................................................

73.2

Basic Camera Settings ................................................................................

83.3

Advanced Settings...................................................................................... 103.3.1
3.3.2

3.4

Image Settings............................................................................... 10Image Enhancement ...................................................................... 11

Image Location .......................................................................................... 123.4.1
3.4.2

Send Images to TWAIN.................................................................. 12
Save in Shared Folder .................................................................... 134 Commissioning and First Operating Steps ....................................... 144.1Installing the TWAIN Plugin ........................................................................ 14

4.2

Installing the TWACKER Demo Application ................................................. 15

4.3

Starting the TWAIN Plugin with TWACKER ................................................. 16

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1 General Information | 1.1 Intended Use

ZEISS

1 General Information
This manual is part of the TWAIN plugin for Axiocam 208 color / 202 mono.

1.1 Intended Use
The TWAIN plugin for Axiocam 208 color / 202 mono is a standardized software interface to callup and control basic camera functions via a TWAIN-compatible non-ZEISS app.

1.2 Contact
If you have any questions or problems, please contact your local ZEISS Sales & Servicepartner or
one of the following addresses:
Headquarter
Phone:+49 1803 33 63 34

Fax:+49 3641 64 3439

Email:

info.microscopy.de@zeiss.com

Service Germany
Phone:+49 7364 20 3800

Fax:

+49 7364 20 3226

Email:

service.microscopy.de@zeiss.com

Courses and training
Email:

courses.microscopy.de@zeiss.com

ZEISS Sales & Servicepartner
You can find a ZEISS Sales & Servicepartner in your area under https://www.zeiss.de/mikroskopie/
website/forms/sales-and-service-contacts.html.

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2 Operation Requirements | 2.1 Hardware

ZEISS

2 Operation Requirements
2.1 Hardware
Component

Requirements

Microscope

The TWAIN plugin can be operated with any microscope the Axiocam 208
color / 202 mono can be connected to.

PC

The TWAIN plugin does not impose any further hardware requirements on
the PC than does the TWAIN-compatible app it is integrated to.

MonitorA display monitor resolution of 1920x1080 is recommended.

2.2 Software
Component

Requirements

CameraFirmware version 1.3.6 or higher must be installed on the Axiocam 208
color / 202 mono.
Note that the latest firmware version can be downloaded fromwww.zeiss.de/mikroskopie/downloads. Consult the Axiocam 208 color /202 mono Instruction Manual on how to update the firmware.

PC§ The PC's operating system must be Microsoft Windows® 7 (32 bit or 64bit) or higher.
§ The application the TWAIN plugin is integrated to must support the image data exchange according to the TWAIN standard.

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2 Operation Requirements | 2.3 Camera Connection Scheme

ZEISS

2.3 Camera Connection Scheme§The camera is connected to the PC for data transfer via its USB interface.

§The camera is connected to the microscope or to the mains for power supply via its Micro-Dinterface.

InfoIf the camera is connected to a coded microscope (e.g. Axioscope 5) via its Micro-D interface,
the microscope's scaling information can automatically be stored with the image and displayed
within it.

Fig. 1: Camera connection to encoded microscope (left) or any other microscope (right)

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3 Software Description | 3.1 General User Interface

ZEISS

3 Software Description
3.1 General User Interface
12

5

3

6

4

7

Fig. 2: TWAIN plugin, basic elements

No.

NameDescription

1

Basic Camera Settings
sectionControls for adjusting basic imaging parameters
[} 8]

2

Advanced Settings section

Controls for adjusting advanced imaging parameters[} 10]

3Live section

Live image of the camera field of view

4

Snap button

Snaps a single image.

5Image Location section

Controls for adjusting the image storing location[} 12]

6Thumbnail section

Gallery of up to 10 temporarily stored images

7Delete button

Deletes the selected thumbnail image.

Tab. 1: TWAIN plugin, basic elements

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3 Software Description | 3.2 Basic Camera Settings

ZEISS

3.2 Basic Camera Settings

1234
567Fig. 3: Basic Camera Settings section

No.
1Name

Description

Auto Exposure checkbox§ Auto Exposure mode (checked): Ensures consistentbrightness of the image by continuously calculating
the correct exposure time based on the current lightintensity.
§ Manual Exposure mode (unchecked): Allows you
to manually re-adjust the exposure time, e.g. whenthe Auto Exposure mode doesn't deliver appropriate
results.

2

Target Value controls

In Auto Exposure mode: Allows you to adjust the lightintensity using the slider or the input field.

3

Exposure Time controls

In Manual Exposure mode: Allows you to adjust the exposure time using the slider or the input field.

4Gain controls

In Manual Exposure mode: Allows you to adjust thegain value using the slider or the input field.

5Gamma controls§ Allows you to adjust the gamma value using theslider or the input field.
§ Allows you to reset the gamma to the default value
using the button.
Note that the default gamma value for Axiocam 208
color is 0.45, the default gamma value for Axiocam
202 mono is 1.2.

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3 Software Description | 3.2 Basic Camera SettingsNo.
6ZEISS

NameDescription

Auto White Balancebutton (for Axiocam 208color)§ Auto White Balance mode (checked): Ensures aconsistent color temperature of the image by continuously calculating the white balance.
§ Manual White Balance mode (unchecked): Allows
you to manually adjust the color temperature, e.g.
when the Auto White Balance mode doesn't deliver
appropriate results.
Note that a proper functioning of the Auto White Balance mode requires enough empty area in the field ofview.

7Color Temperatureslider (for Axiocam 208color)

In Manual White Balance mode: Allows you to adjust
the color temperature using the slider.

Tab. 2: Basic Camera Settings section, control elements

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3 Software Description | 3.3 Advanced Settings

ZEISS

3.3 Advanced Settings
3.3.1 Image Settings

123Fig. 4: Advanced Settings section, Image Settings tabNo.
1

Name

Description

Image Format control

Sets the file format of captured images.
§ BMP
§ JPG

2

Snap Resolution control

Sets the resolution of captured images.
§ 1080P§ 4K

3Scale Bar control

Activates/deactivates the display of a scale bar in thelive image and the captured image.
Note that the display of the scale bar requires the camera's being connected to a coded microscope (e.g. Axioscope 5) via its Micro-D interface.

Tab. 3: Advanced Settings section, Image Settings tab, control elements

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3 Software Description | 3.3 Advanced Settings

ZEISS

3.3.2 Image Enhancement

123Fig. 5: Advanced Settings section, Image Enhancement tab

No.

NameDescription

1

Denoise control

Activates/deactivates the automatic noise reduction.

2

Sharpen control

Activates/deactivates the automatic sharpening of details.

3Exposure Mode control(for Axiocam 208 color)Sets the imaging mode.
§ Normal/bright-field§ FluorescenceTab. 4: Advanced Settings section, Image Enhancement tab, control elements

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3 Software Description | 3.4 Image Location

ZEISS

3.4 Image Location
3.4.1 Send Images to TWAIN
When the tab is selected, the acquired images are sent to the basic application via TWAIN interface.

1Fig. 6: Image Location section, Send Images to TWAIN tabNo.
1NameDescription

Close window automatically after snapping checkbox

Activates/deactivates the automatic closing of theplugin's user interface after each image capturing.

Tab. 5: Image Location section, Send Images to TWAIN tab, control elements

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3 Software Description | 3.4 Image Location

ZEISS

3.4.2 Save in Shared Folder
When the tab is selected, the acquired images are stored to the specified folder.

12Fig. 7: Image Location section, Save in Shared Folder tabNo.

NameDescription

1Browse button

Opens a file explorer window to select the desired target folder.

2Target Folder display

Displays the currently selected target folder to store images.

Tab. 6: Image Location section, Save in Shared Folder tab, control elements

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4 Commissioning and First Operating Steps | 4.1 Installing the TWAIN Plugin

ZEISS

4 Commissioning and First Operating StepsInfo
You need administrator rights to install software on your laboratory PC. Consult your IT administrator for the installation of the software.

4.1 Installing the TWAIN Plugin
1. Go to www.zeiss.com/microscopy/int/downloads.
2. Select Software Products from the drop-down list.
3. Select TWAIN from the appearing second drop-down list.
à A list of available downloads appears.
4. Click on the TWAIN installation file applicable for your operating system version.
à The installation file is downloaded.
5. Open your Downloads folder and double-click on the TWAIN installation file (file extension .exe).
à The installation wizard opens.
6. Follow the instructions of the wizard.
à After the installation is finished, the camera driver installation wizard opens.
7. Follow the instructions of the wizard.
8. Consult the basic application's customer support for help with integrating the plugin intothe application.

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4 Commissioning and First Operating Steps | 4.2 Installing the TWACKER Demo Application

ZEISS

4.2 Installing the TWACKER Demo Application
To demonstrate the image acquisition with the TWAIN plugin you can use the TWACKER application. This demo application can also be downloaded from the ZEISS website.

InfoTWACKER is not mandatory for operating the TWAIN plugin. If your laboratory software supports the TWAIN standard, you don't need to install TWACKER.
1. Go to www.zeiss.com/microscopy/int/downloads.
2. Select Software Products from the drop-down list.
3. Select TWAIN from the appearing second drop-down list.
à A list of available downloads appears.
4. Click on the TWACKER installation file applicable for your operating system version.
à The installation file is downloaded.
5. Open your Downloads folder and double-click on the TWACKER installation file (file extension .msi).
à The installation wizard opens.
6. Follow the instructions of the wizard.

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4 Commissioning and First Operating Steps | 4.3 Starting the TWAIN Plugin with TWACKER

ZEISS

4.3 Starting the TWAIN Plugin with TWACKERThe TWACKER application can be used to demonstrate the calling and operating of the TWAIN
plugin.
Prerequisite ü The camera is properly connected. [} 6]ü The TWAIN plugin and the TWACKER application are installed on the PC.
1. From the Windows® Start menu, select Twack_64 or Twack_32, according to your operating system version.
à The TWACKER application window opens.
2. From the application's File menu, select theSelect Source... entry.

à The Select Source window opens.
3. From the window's devices list, select ZEISSTWAIN.

4. Click the Select button.

5. From the application's File menu, select theAcquire... entry.

The TWAIN plugin user interface opens and is ready for operating the camera.

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Quick Guide ZEISS TWAIN for Axiocam 202/208 | en-US | Rev.1
Modifications reserved.
Carl Zeiss Microscopy GmbHCarl-Zeiss-Promenade 1007745 Jena
Germanyphone: +49 3641 64 3161
fax: +49 3641 64 3439
info.microscopy@zeiss.com
www.zeiss.com/microscopy