৙ẼɥậෙᕹỉஊјМဇᴾὼ༏൦ਁЈཋỉἱ἟ἻἽẆ‫ٶ‬ኄ᫏Ẇ৴ᣠ҄෇ࣱὼᴾᴾ‒

⾲㸰ᡴࡕୖࡆᾏ⸴ᢳฟᾮࡢ࢖࢜ࣥ⤌ᡂ PPRO/ CationAnion
Japanese nameNH 4 NaK
Mg Ca H 2 PO4 Cl
Phaeophyceae
Laminariaceae
Ecklonia stolonifera
Tsuru-arame
1.67 40.63 27.09 3.04 1.70 0.46 35.29
E. kurome
Kurome2.00 44.54 27.62 3.62 1.97 0.35 40.53
Sargassaceae
Sargassum ringgoldianum
subsp. coreanum
Yanagi-moku- 45.32 69.16 7.82 4.09 4.36 104.8
S. macrocarpum
Nokogiri-moku 0.83 27.71 39.80 5.55 2.30 3.86 63.10
S. silliquastrum
Yore-moku
0.71 33.28 33.10 4.69 1.92 3.97 60.62
S. confusum
Fushisuji-moku 0.83 26.05 32.53 4.28 1.50 1.07 51.31
S. patens
Yatsumata-moku 0.72 16.79 15.14 6.21 1.30
- 20.45
S. piluliferum
Mame-tawara
0.67 25.92 16.85 4.07 1.50 3.04 36.13
Chlorophyceae
Ulvaceae
Ulva pertusa
Ana-aosa
0.67 16.66 20.44 16.21 1.67
9.45
Enteromorpha intetinalis Usubaaonori
6.44 12.35 17.62 8.80 1.27 3.09 9.48
Cladophoraceae
Chaetomorpha moniligera Tamajyuzumo 0.56 10.00 13.58 1.32 0.90
14.27
Codiaceae- 61.77
Codium fragile
Miru1.06 52.46 5.86 6.62 1.8546810min

Cm
Cf

14016

81012min14

Yanagi-moku(Sr)6420681012min14ᅗ㸴㸬 ࣖࢼࢠࣔࢡᒚ∝௑ SargassumStem ringgoldianum
 ssp. coreanum ࡢᙧែ
ఌ Holdfastᩘ㛵ᩘⓗ䛷䛒䛳䛯䚹㻌䖃䝲䝘䜼䝰䜽⸴య㒊ศู䛾⢓ᗘ䛚䜘䜃Ỉ⁐ᛶከ⢾㢮㻌㻌 ୖ㏙䛾䜘䛖䛻ᡴ䛱ୖ䛢㔞䛜ከ䛟䚸䝭䝛䝷䝹䚸ከ⢾㢮䚸䝫䝸䝣䜵䝜䞊䝹ྵ㔞䚸ᢠ㓟໬άᛶ䛾㧗䛔䝲䝘䜼䝰䜽䛻䛴䛔䛶ⱼ䞉ᯞ㒊䚸ⴥ㒊䛚䜘䜃ୖ㒊䛻ศ䛡䛶䠄ᅗ䠒䠅⇕Ỉᢳฟ䜢⾜䛔䚸ྠᵝ䛻᳨ウ䛧䛯䚹ୖ㒊䛿ⴥ㒊䛸ྠᵝ䛻ከ⢾㢮ྵ㔞䛚䜘䜃┦ᑐ⢓ᗘ䛜㧗䛛䛳䛯䛜䚸ⱼ䞉ᯞ㒊䛷䛿ప䛔್䛷䛒䛳䛯(ᅗ䠓)䚹䜎
䛯䚸ୖ㒊䛛䜙䛾ᢳฟᾮ䛻䛿䝣䝁䜲䝎䞁䛜ྵ䜎䜜䛶䛔䛯䚹
 50

1640

Sr

10

r2 = 0.931

8Spa

6mV

Relative viscositymV612104Sm

2GFC(HPLC) chromatography

Ek20

Es

01603012108Upper

Frond64 Stem
20Monosaccharide

High molecularpolysaccharide

0.20.40.60.8High molecular polysaccharide
mg alginate Eq/mL

10Laminaranᅗ㸱ᾏ⸴ከ⢾㢮ࡢࢤࣝࢁ㐣ࢡ࣐ࣟࢺࢢ࣒ࣛ *)&   ࡜┦ᑐ⢓ᗘ࡜ࡢ┦㛵ᛶ

051015min20ᅗ㸵㸬ࣖࢼࢠࣔࢡ㒊఩ูࡢ *)& ࡜┦ᑐ⢓ᗘSc

Ss200
Ek
EsSpa

Cf

0200

Up

Stem

200
150

r2 = 0.980

00102030

40

50

300

350

400
Radical scavenging (%)

2015

㻟㻜80
㻝㻜60㻝㻜㻞㻜㻟㻜㻠㻜㻡㻜100

r2 = 0.611

8060
40
20004010Sm Ss Sc Spa Spi Cm Up Ei

OH᳽
radical6040Cf80Sr

Ek Es Up Sm Ss Sc Ei Spa Spi Cf

r2 = 0.8808
20r2 = 0.9878

20150101020304050

Reducingpower(mol PGEq᳽mL-1 )Cm

Sm Ss Sc Spa Spi Up Ei
Samples

Cm Cf

020

r2 = 0.963

1510501020304050(mol PGEq᳽mL-1 )Ek Es Sr

100DPPH䞉Fe-reducing
powerSuperoxide anion䞉
280
60

140: Stem: Frond䕿: Upper䕿䕿200110100

1101001000

01mL sample/Lᅗ㸷㸬ࣖࢼࢠࣔࢡ㒊఩ูࡢᢠ㓟໬≉ᛶ

101001000mL sample/L

25

Total phenols

5400

300Total phenols

Es Ek Sr

50025600204030100

4030(mol PGEq᳽mL-1 )(mol CatEq᳽mL-1 )Es Ek Sr

100

20Total phenols

20

580

120

Total phenols(mol PGEq᳽mL-1 )0radical100
㻞㻜

㻜
㻜

10OO᳽

120

r2 = 0.958140(mol CatEq᳽mL-1 )radical25(mol CatEq᳽mL-1 )DPPH᳽

Radical scavenging

30

Radical scavenging

140
㻠㻜

350

Wave length (nm)ᅗ㸶㸬ࣖࢼࢠࣔࢡ㒊఩ูࡢ⣸እ⥺྾཰࡜ࣇ࢙ࣀ࣮ࣝ໬ྜ  ≀ྵ㔞(mol PGEq᳽mL-1 )ᅗ㸲ᾏ⸴ᢳฟᾮࡢ⣸እ⥺྾཰࡜ࣇ࢙ࣀ࣮ࣝ໬ྜ≀  ྵ㔞࡜ࡢ┦㛵
35300

Upperpart

0200

50

Wave length (nm)

40

250

200

100

Total phenols

250

Frond

Phenolic compounds
mmol PGEq/L
Stem
33.4㼼0.5
Frond
31.9㼼0.4
Upper part 15.7㼼0.4

300

100

Sr

Ei SpiCm

400

OD 700nm

Absorbance

Sm300䖃䝲䝘䜼䝰䜽⸴య㒊ศู䛾ᢠ㓟໬≉ᛶ㻌 ⱼ䞉ᯞ㒊䛾䝫䝸䝣䜵䝜䞊䝹ྵ㔞䚸UV280nm 䛾྾཰(ᅗ䠔)䚸
䛚䜘䜃ᢠ㓟໬άᛶ(ᅗ䠕)䛿ⴥ㒊䛸㏆䛔್䛷䚸ୖ㒊䛷䛿䝫䝸䝣䜵䝜䞊䝹ྵ㔞䛜ⴥ㒊䛾⣙ 1/2䚸ᢠ㓟໬άᛶ䛿 1/4䡚1/5 ⛬ᗘ䛷䛒䛳䛯䚹Absorbance

400

Absorbance at 280 nm䖃䝫䝸䝣䜵䝜䞊䝹ྵ㔞䛚䜘䜃ᢠ㓟໬άᛶ㻌㻌 䜹䝆䝯㢮䛷䝫䝸䝣䜵䝜䞊䝹ྵ㔞䛜㧗䛟(45-47㻌 μmol 䝣䝻䝻䜾䝹䝅䝜䞊䝹ᙜ㔞(PGEq/mL))䚸䛴䛔䛷䝲䝘䜼䝰䜽䛷
㧗䛟䚸UV280 ௜㏆䛾྾ගᗘ䛸Ⰻ䛟┦㛵䛧䛯(ᅗ䠐)䚹DPPH䝷䝆䜹䝹ᤕᤊ⬟䛚䜘䜃㕲㑏ඖ⬟䛿䝫䝸䝣䜵䝜䞊䝹ྵ㔞䛸┦㛵䛧䛶䛚䜚䚸䜹䝆䝯㢮䛷ᙉ䛔άᛶ䜢♧䛧䛯䠄ᅗ䠑䠅䚹୍᪉䚸䝇䞊䝟䞊䜸䜻䝅䝗䜰䝙䜸䞁䝷䝆䜹䝹ᤕᤊ⬟䛿䝫䝸䝣䜵䝜䞊䝹ྵ㔞䛸䛾┦㛵䛿䛒䜎䜚㧗䛟䛺䛟䚸䝲䝘䜼䝰䜽䛜᭱䜒㧗䛔άᛶ(120 μmol CatEq/mL)䜢♧䛧䛯䚹䝠䝗䝻䜻䝅䝹䝷䝆䜹䝹ᤕᤊ⬟䛿䝫䝸䝣䜵䝜䞊䝹ྵ㔞䛻ᑐ䛧䛶ᣦ

0Up
Ei

0.22
3.96

15
10

Reducing power

Sr
Sm
Ss
Sc
Spa
Spi

12(mol CatEq᳽mL-1 )

Es
Ek

3.14
2.94

Tsuru-arame
(Es)

20

∊⇰⇔∈⇕

2.45
1.40
1.91
1.17
0.51
4.25

50

Radical scavenging

Japanese name Local name

Phaeophyceae
Laminariaceae
Ecklonia stolonifera
Tsuru-arame
KajimeEdible; Bath agent
E. kurome
KuromeKajimeEdible; Bath agent
SargassaceaeSargassum ringgoldianum
subsp. coranum Yanagi-moku
Fertilizer
S. macrocarpum
Nokogiri-moku
Fertilizer
S. silliquastrum
Yore-moku
Fertilizer
S. confusum
Fushisuji-moku
S. patens
Yatsumata-moku(Edible); Fertilizer
S. piluliferum
Mame-tawara
Fertilizer
Chlorophyceae
Ulvaceae
Ulva pertusa
Ana-aosa
Aosa, Aonori Edible; Fertilizer
Enteromorpha intetinalis Usuba-aonori Aonori, Aosa Edible
Cladophoraceae
Chaetomorpha moniligera Tama-jyuzumo
Codiaceae
Codium fragile
Miru
EdiblemV62mol CatEq᳽mL-1

Scientific nameAbbreviationEdible and/orin Figs
other applications

3025

Alginic acid 780k LaminaranDP䍦228100䖃⇕Ỉᢳฟᾮ䛾䜲䜸䞁⤌ᡂ㻌 ᵝ䚻䛺ᾏ⸴䛜ᡴ䛱ୖ䛢䜙䜜䛶䛔䛯䛜䚸㔞䛜ከ䛟䚸฼⏝ྍ⬟䛸⪃䛘䜙䜜䛯䜒䛾䛿⾲ 1 䛻♧䛩〓⸴㢮 8 ✀䚸≉䛻䚸䜹䝆䝯㢮䠄䝒䝹䜰䝷䝯䚸䜽䝻䝯䠅䛸䚸䝩䞁䝎䝽䝷㢮䛾䝲䝘䜼䝰䜽䚸䝜䝁䜼䝸䝰䜽䚸䝶䝺䝰䜽䛷䛒䛳䛯䚹㻌 ሷ⃰ᗘ(⾲䠎)䛿䝲䝘䜼䝰䜽䛚䜘䜃䜹䝆䝯㢮䛷㧗䛟䚸Na 䛻ᑐ䛩䜛 K 䛾䝰䝹ẚ䛿䝲䝘䜼䝰䜽
䛷㧗䛟(1.53)䚸䜹䝆䝯㢮䛷ప䛛䛳䛯䚹㻌⾲㸯㸬ᮏᐇ㦂࡟⏝࠸ࡓᾏ⸴✀

3.04
3.62

Relative viscosity108ኽௐấợỎᎋ‫ݑ‬ᴾɥᢿ
Upperpartᓶ
FrondSO4㻌䖃┦ᑐ⢓ᗘ䚸Ỉ⁐ᛶከ⢾㢮䠄㣗≀⧄⥔䠅㻌㻌 Ỉ䛻ᑐ䛩䜛┦ᑐ⢓ᗘ䛿䝲䝘䜼䝰䜽(10.3䠅䚸䝲䝒䝬䝍䝰
䜽(6.3)䛚䜘䜃䜰䝘䜰䜸䝃(4.7)䛷㧗䛟䚸䛭䛾௚䛾䝃䞁䝥䝹䛷䛿 2.2 ௨ୗ䛷䛒䛳䛯䚹〓⸴㢮 8 ✀䛾┦ᑐ⢓ᗘ䛿㧗ศᏊከ⢾㢮䠄䜰䝹䜼䞁㓟䠅ྵ㔞䛸┦㛵䛧䛶䛚䜚䠄ᅗ䠏䠅䚸䜹䝆䝯㢮䛾ᢳฟᾮ䛻䛿㈓ⶶከ⢾㢮䛾䝷䝭䝘䝷䞁(β 1,3:1,6 䜾䝹䜹䞁)䛜ከ䛟ྵ䜎䜜䛶䛔䛯䚹

᬴ܱ૾ඥᴾ㻌 2007 ᖺ 10 ᭶䠄㞵ኳ䠅䛻ᅗ 2 䛻♧䛩 8 ᆅⅬ䛾ᾏᓊ䠄●䠅䛻ᡴ䛱ୖ䛢䜙䜜䛯ᾏ⸴ 12 ✀䠄⾲ 1䠅䜢᥇㞟䛧䛯䚹⏕㩭ⴥ㒊ヨᩱ䛻䠐ಸ㔞䛾Ỉ䜢ຍ䛘⣽᩿䛧䚸80䉝䛷 30 min ᢳฟ䛧䛯Ỉ⁐ᾮ䜢䝃䞁䝥䝹⁐ᾮ䛸䛧䛯䚹䝃䞁䝥䝹⁐ᾮ୰䛾䜲䜸䞁⤌ᡂ䛿䜲䜸䞁ศᯒᶵ䠄PIA-1000䚸Shimadzu䠅䛷ศᯒ䛧䛯䚹┦ᑐ⢓ᗘ䛿᣺ືᘧ⢓ᗘィ䠄VM-1G䚸ᒣ୍㟁ᶵ䠅䜢⏝䛔䛶 ᐃ䛧䚸Ỉ⁐ᛶከ⢾㢮䛾䛚䛚䜘䛭䛾ྵ㔞䛸ᖹᆒศᏊ㔞䜢䜰䝹䝁䞊䝹ỿẊἲ䛚䜘䜃䝀䝹䜝㐣(GFC) HPLC ἲ䛻䜘䜚 ᐃ䛧䛯䚹䝫䝸䝣䜵 䝜 䞊 䝹 ྵ 㔞 䠄 Folin-Ciocalteu ἲ 䠅 䛚 䜘 䜃 ᢠ 㓟 ໬ ά ᛶ䠄DPPH 䝷䝆䜹䝹䚸䝇䞊䝟䞊䜸䜻䝅䝗䜰䝙䜸䞁䝷䝆䜹䝹䠄㠀㓝⣲ⓗ NBT-PMS ἲ䠅䚸䝠䝗䝻䜻䝅䝹䝷䝆䜹䝹ᤕᤊ⬟䠄Fenton཯ᛂ䠅䚸䛚䜘䜃㕲㑏ඖ⬟䠅䛿᪤ሗ䛻ἢ䛳䛶䝬䜲䜽䝻䝥䝺䞊䝖ἲ䛷 ᐃ䛧䛯䠄n = 3䠅䚹
Kinoura KawauraMaura㻌
Sosogi㻌Mitsuko-hama㻌㻌㻌㻌Misaki-Awazu
㻌
Mitsuke-jima㻌
Koiji-hama㻌㻌ᅗ ᡴࡕୖࡆᾏ⸴᥇ྲྀᆅⅬ㻌‒

Scientific namemV㻌 ⬟Ⓩ༙ᓥἢᓊ䛷䛿ᵝ䚻䛺ᾏ⸴䛜⦾ⱱ䛧䚸䛸䛟䛻䝩䞁䝎䝽䝷㢮䛿Ⰻ㉁䛺⸴ሙ䜢ᙧᡂ䛩䜛ୖ䛷㔜せ䛷䛒䜛䚹䛭䛾୍㒊䛿ὶ䜜⸴䛸䛺䛳䛶䜒䚸ᑠᆺື≀䛻⏕⫱⎔ቃ䜢ᥦ౪䛧䛺䛜䜙䚸὾䛻ᡴ䛱ୖ䛢䜙䜜䜛䚹෤䛛䜙ኟ䛻ᡴ䛱ୖ䛢䜙䜜䜛䝒䝹䜰䝷䝯䚸䜽䝻䝯䚸䜰䜹䝰䜽➼䛾〓⸴㢮䛿㣗⏝䛸䛧䛶᥇ྲྀ䛥䜜䜛䛜䚸㣗⏝௨እ䛾ᾏ⸴䜔㣗⏝䛻㐺䛥䛺䛔Ꮨ⠇䛾ᾏ⸴䠄ᅗ䠍䠅䛿䚸୍㒊䛜⫧ᩱ䛸䛧䛶⏝䛔䜙䜜䜛䜒䛾䛾䚸኱༙䛿䝂䝭䛸䛧䛶ᆅඖఫẸ䛻䜘䛳䛶ฎศ䛥䜜䛶䛔䜛䚹ᮏ◊✲䛷䛿䚸䛭䛾䜋䛸䜣䛹䛜᭷ຠ฼⏝䛥䜜䛶䛔䛺䛔⛅Ꮨ䛾ᡴ䛱ୖ䛢ᾏ⸴䛻䛴䛔䛶䚸᭷ຠά⏝䛾ྍ⬟ᛶ䜢᳨ウ䛩䜛䛯䜑䛻䚸⇕Ỉᢳฟᾮ୰䛾䜲䜸䞁䠄䝭䝛䝷䝹䠅䚸Ỉ⁐ᛶከ⢾㢮ྵ㔞䜔ᢠ㓟໬≉ᛶ➼䛻䛴䛔䛶᳨ウ䛧䛯䚹㻌⁀⁓⁘⁧⁠⁗‽⁛⁠⁡⁧⁤⁓㻌‪‡‣․‡• ‒‣…‡‣‣‡•㻌㻌㻌㻌㻌㻌㻌㻌㻌㻌㻌㻌ᅗ ㍯ᓥ⌔Ὢࡢᡴࡕୖࡆᾏ⸴㻌‒

Sm Ss Sc Spa Spi Ei
SamplesCm Up Cfᅗ㸳⇕Ỉᢳฟᾮࡢᢠ㓟໬ᛶ㸦'33+ ࣛࢪ࢝ࣝᤕᤊ⬟ ࢫ࣮ࣃ࣮࢜࢟ࢩࢻ࢔ࢽ࢜ࣥࣛࢪ࢝ࣝᤕᤊ⬟ࠊࣄࢻࣟ ࢟ࢩࣝࣛࢪ࢝ࣝᤕᤊ⬟ࠊ㕲㑏ඖ⬟㸧࡜ࣇ࢙ࣀ࣮ࣝ໬ ྜ≀ྵ㔞ࡢ┦㛵㻌 ௨ୖ䛾䜘䛖䛻⌧ᅾ᫬໬䠄䛧䛡䠅䛾⩣᪥䛻⁻╔䛧䚸኱㔞䛾䝂䝭䛸䛺䜛ᡴ䛱ୖ䛢ᾏ⸴䛾୰䛻䛿䚸䝭䝛䝷䝹䚸Ỉ⁐ᛶከ⢾㢮䠄㣗≀⧄⥔䞉ಖ‵ᛶ䠅䛜ከ䛟䚸㼁㼂 ྾཰⬟䛚䜘䜃ᢠ㓟໬άᛶ䛾㧗䛔䜒䛾䛜䛒䜛䚹≉䛻䝲䝘䜼䝰䜽䛻䛴䛔䛶䛿䚸ᡓ᫬୰䛾㣫ⴥ䜔㠀ᖖ㣗䛸䛧䛶౑⏝䛥䜜䛯䛣䛸䜒ఏ䛘䜙䜜䛶䛚䜚䚸௒ᚋ䛿䛥䜙䛻ᶵ⬟ᛶ䜔Ᏻ඲ᛶ䚸฼⏝ἲ䛻䛴䛔䛶䜒᳨ウ䛧䛯䛔䚹㻌㻌㻌 ᮏ◊✲䛾୍㒊䛿㈈ᅋἲேᆅᇦ⥲ྜᩚഛ㈈ᅋ䠄䜅䜛䛥䛸㈈ᅋ䠅䛚䜘䜃⌔Ὢᕷ䛾ຓᡂ䜢ཷ䛡䛶㐙⾜䛥䜜䛯䚹㻌