MuVi-SPIM

Life in the Fast Lane
Large Field of View
Close-to-confocal Resolu�on
Very High Imaging Speed
Gentle Sample Handling and Low Photoxicity
High Sensi�vity and Low Noise360⁰ Illumina�on and Detec�onMuVi-SPIM
The Technology
The MuVi-SPIM u�lizes a sheet of laser light to illuminate only a thin sliceof a ﬂuorescently labeled sample. A wide-ﬁeld ﬂuorescence microscope, placed
perpendicular to the light-sheet, serves to collect the ﬂuorescence signal andimages the observed region by means of a camera.

The MuVi-SPIM provides four simultaneous orthogonal views on large living
specimens without the need for sample rota�on. This avoids shadowing eﬀects
and facilitates long-term imaging at drama�cally increased acquisi�on speed.
Modular so�ware concepts allows the ﬂexible design of complex experimental
layouts. 3D image data are used in real-�me and directly streamed to a storage
and data processing server.

MuVi-SPIM system uses some unique and somepatented technologies. The four objec�ve lenses
allow four combina�ons of illumina�on and detec�on.
This provides four orthogonal views of the specimen
without the need for sample rota�on. It enablesmul�ple view imaging of large samples at unprecedented speed with long-term stability.

Fast Imaging
Large ﬁeld of view, close-to
confocal resolu�on, very
high imaging speed, highest
sensi�vity and minimum
noise.

Seeing life from adiﬀerent perspec�ve-or four
3D imaging of large specimen
with light-sheet illumina�on,gentle sample handling.

A fruit ﬂy embryo from when it was about two-and-a-half hours oldun�l it walked away from the microscope as a larva, acquired bya MuVi-SPIM system.
Light Sheet Microscopy
Excellent signal-to-noise ra�o,lowphotobleaching andphotoxicity.

3D construc�on images of zebraﬁshand zebraﬁsh eye, acquired by a
MuVi-SPIM system.

360� illumina�onand detec�onIntrinsic 3D op�cal sec�oning,More movies can be viewed in SANE ASIA YouTubechannel.

high �me resolu�on, longterm stability, real-�me data
fusion and visualiza�on.

Speciﬁca�ons
Laser

Detec�on Op�cs

Laser combiner with six laser posi�ons
445, 488, 515, 532, 561, 594, 642, and 685nm, 50mW
Fast modula�on and high ex�nc�on
Chroma�c correc�on from 440 to 660 nm
Light-sheet genera�on by beam scanning
Variable light-sheet thickness (op�onal)
Water-dipping objec�ve lenses
Fast ﬁlter wheels with 10 posi�ons and 50ms between
adjacent posi�ons
Variable magniﬁca�on (op�onal)
High-speed sCMOS camera Hamamatsu ORCA-Flash 4.0
Confocal mode available

Objec�ve Lens

2x Nikon CHI Plan Fluor 10XW 0.3NA water immersion forMoun�ng Unitillumina�on2x Nikon CFI LWD 25XW 1.1NA water immersion for
detec�onMoun�ng Chamber with temperature control (15�C - 40�C)
Other objec�ve lenses upon request

Sample Chamber

Water-sealed inert PEEK plas�c chamber, autoclavable andand Stage

biocompa�bleSample supported from below for improved stability
Easy access from above for sample moun�ng etc.
Fast and precise temperature control, range 15-40�C
XYZ piezo crawler stage with 100nm resolu�on andSystem Control

8.5mm(X) and 1.2mm (Y) travel range
High precision XYZ and fast rota�on stage
Embedded microscope so�ware with open
communica�on interface
Open GUI control for interface control and microscope
automa�on
High-speed RAID controller for data streaming
HIVE High Speed Centralized Data System (Op�onal)h�p://www.saneasia.com