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Product Name : Ascorbate oxidase (ASO-301)

First issue : Oct. 29. 2014
Revised: May. 31. 2022
SDS No. 944F

Safety Data Sheet
1 Chemical Product and Company Identification
Identification of the productProduct CodeSupplier
NameAddress
Department
Phone
Fax
Recommended use and restrictions on use

Ascorbate oxidase
ASO-301TOYOBO CO.,LTD.
Osaka Umeda Twin Towers South,
1-13-1 Umeda Kita-ku, Osaka 530-0001, Japan
Biotechnology Overseas Sales and Marketing Department+81-6-6348-3846+81-6-6348-3833
diagnostic product2 Hazard Identification
Important hazards
Hazards and Effect for human health
GHS classification
Physical hazards
Health hazards
Acute toxicity : Oral
Acute toxicity : Dermal
Acute toxicity : Inhalation (Gas,Vapour)
Acute toxicity : Inhalation (Dust,Mist)
Skin corrosion/irritation
Serious eye damage/Eye irritation
Sensitization : Respiratory
Sensitization : Skin
Germ cell mutagenicity
Carcinogenicity
Toxic to reproduction
Specific target organ toxicity(Single exposure)Specific target organ toxicity(Repeated exposure)This product might be harmful if swallowed, because itcontains boric acid and sodium tetraborate.
Lethal dose (Boric acid) : Adult 10g, Child 5g
Category 5
Not applicable
Category 2
Category 2ACategory 1B
Category 1(nervous system, gastrointestinal tract,respiratory organs, kidneys)
Category 1(nervous system, respiratory organ, kidneys)
Category 2(testis)

Aspiration hazard
Environmental hazards
Acute hazards to the aquatic environmentLong-term hazards to the aquatic environment Hazard to the ozone layer-:Classification not possible
GHS Label Elements
Symbol/Pictograms

Signal wordHazard statements

DangerMay be harmful if swallowed.
May cause skin irritation.
May cause serious eye irritation.
May damage fertility or the unborn child.
Cause damage to nervous system, gastrointestinal tract,respiratory organ, and kidneys.
Cause damage to nervous system, respiratory organ,
kidneys through prolonged or repeated exposure.

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Product Name : Ascorbate oxidase (ASO-301)

First issue : Oct. 29. 2014
Revised: May. 31. 2022
SDS No. 944F

Safety Data Sheet
Precautionary statements

May cause damage to testis through prolonged or repeatedexposure.
Prevention Obtain special instructions before use.
Do not handle until all safety precautions have been readand understood.
Wear protective gloves/protective clothing/eye
protection/face protection.
Do not breathe dust/fume.
Do not eat, drink or smoke when using this product.
Wash hands thoroughly after handling.
Response Get medical advice/attentions if you feel unwell.
If ON SKIN: Wash with plenty of water/soap. Take off
contaminated clothing and wash it before use.

Important symptoms (Boric acid)3

Composition/Information on Ingredients
Substance/Mixture
Chemical Nature
Chemical specificity
Concentration or concentration range
Cas #Ingredients Contributing to the Hazard
Common Chemical nameChemical formula
Cas #Common Chemical nameChemical formula
CAS#
Boron content of this product4

If skin irritation occurs: Get medical advice/attention.
If in eye: Rinse cautiously with water for several
minutes. Remove contact lenses, if present and easy todo. Continue rinsing. If eye irritation persists, getmedical advise/attention.
IF exposed or concerned: Get medical attention/advice.
Storage Store locked up.
Store in a well-ventilated place. Keep container tightlyclosed.
Disposal Dispose of contents/container in accordance withlocal/regional/national/international/regulation.
Symptoms of vomit, diarrhea, collapse, erythema may
appear after 2-3 hours later and die after 3-5days laterfrom ingestion.

MixtureAscorbate oxidase
Freeze and drying powder including enzyme
ca. 11 %(W/W)
9029-44-1
Boric acid
H3BO3
10043-35-3
Sodium tetraborate decahydrate
Na2B4O7・10H2O
1303-96-4
ca. 19 %(W/W)

First Aid Measures
Inhalation
Skin Contact
Eye Contact

Ingestion

Remove to fresh air. Consult a physician whenunpleasantness occurs.
Wash off with plenty of water. Consult a physician wheninflammation on the skin occurs.
Rinse cautiously with water for several minutes. Remove
contact lenses,if present and easy to do.Continue
rinsing.
Rinse mouth. Swill plentiful amount of water or milk for
immediate vomiting. Consult a physician.

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Product Name : Ascorbate oxidase (ASO-301)

First issue : Oct. 29. 2014
Revised: May. 31. 2022
SDS No. 944F

Safety Data Sheet
Most important symptoms/effects, acute anddelayed.

Inhalation: cough, pant, pharygeal pain, nosebleed.
Skin: dry skin.
Eye: redness, pain
Ingestion: stomachache, derangement, diarrhea, headache,nausea, vomit, lassitude, cramp.

Protection for first-aiders
Notes to an attending physician

Wear protective equipments depending on the situation.
Rest and medical observation are nesessary.

5 Fire Fighting Measures
Extinguishing media
Specific hazards
Specific extinguishing methods
Precautions for fire-fighters

This product is not flammable. Use extinguishing agent
suitable for type of surrounding fire.
No information available.
Water spray,dry chemical powder,or carbon dioxide etc.
Firefighter should work from the windward side.

6 Accidental Release Measures
Personal Precautions, protective equipment
and emergency procedure.
Environmental Precautions
Methods and materials for containment andcleaning up.

Wear protective gear to avoid eye/skin contact andinhalation. Do not work at leeward.
High concentrated waste fluid should not be directly
discharged into rivers.
Take up under vacuum using dust collecting filter, wash
residual spill with copious amounts of water. Use
cloth, paper or anything similar to soak up thesolution leaking out of the container.
(Waste water should be treated with activated sludge oradsorbed with activated carbon etc.)7 Handling and Storage
Handling
Technical Measures
Precautions

Wear protective gear to avoid eye/skin contact andinhalation.
Do not drop the container to prevent the content poppingout.

StorageTechnical Measures
Incompatible substances and mixtures
Storage Conditions
Packaging Materials

Keep sealed container in freezer.
None specified.
Store under -20℃ to avoid deactivating.
Use the initial container of the product.

8 Exposure Controls/Personal Protection
Engineering controls
Occupational exposure limit,biological limit
ACGIH TLV(2004)
OSHANIOSH
Personal Protective Equipment
Respiratory Protection
Hand Protection
Eye ProtectionSkin and Body Protection

Provide shower and eye washing apparatus nearby.
(Borate compounds, inorganic) TWA 2mg/m3, STEL 6mg/m3
Not established.

Borates, tetra, sodium salts, pentahydrate REL:1mg/m3 TWA
Protecting mask
Protecting gloves
Safety goggles
Long sleeve working wear

9 Physical and Chemical Properties
Physical State, form and color
Odour
pH
Flash Point
ExplosivenessPowder / Light blue
No odourca. pH 8.5 (1% W/V)
No information available.
No information available.

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Product Name : Ascorbate oxidase (ASO-301)

First issue : Oct. 29. 2014
Revised: May. 31. 2022
SDS No. 944F

Safety Data Sheet
Density
Solubility

No information available.
Freely soluble in water.

10 Stability and Reactivity
Stability
Stable at temperatures below -20℃.When left for long at
Possible hazard reactions at specific condition room temperature,proteins might be degraded, which does
not cause any hazardous reaction.
Conditions to avoid
Materials to avoid
Hazardous Decomposition Products

Prolonged storage under higher temperature than room
temperature and high humidity.
Oxidizing agent
No information available.

11 Toxicological Information(1) Boric acid
Acute Toxicity (Oral)
Skin corrosion/irritation
Serious eye damage/irritation
Sensitization
Germ cell mutagenicity

Carcinogenicity
Toxic to reproduction

Specific target organ toxicity(Single exposure)Specific target organ toxicity(Repeated exposure)(2) Sodium tetraborate
Acute Toxicity (Oral)
Acute Toxicity (Dermal)
Skin corrosion/irritation
Serious eye damage/irritation
Toxic to reproduction
Specific target organ toxicity(Single exposure)Specific target organ toxicity(Repeated exposure)
Aspiration hazard

Rat LD50 2660mg/kg
Moderate irritation (guinea pig, 24hr, 72hr)
Rubefaction /Pain
No data available
Absence of data on multi-generation mutagenicity tests
and germ cell mutagenicity tests in vivo, and negative
data on somatic cell mutagenicity tests in vivo
(micronucleus tests)
ACGIH (2005): Category A4Adverse effects on reproduction of parental animals anddevelopment of pups at doses producing no parental
toxicity.
Human:Gastrointestinal tract effects such as nausea,vomiting, abdominal pain and diarrhea, and central nerve
effects such as lethargy, headaches, fever, increased
irritability and muscle convulsion.
Irritation of the upper respiratory tract.
Animal:cyanosis, tetany, spasm and shock-like symptoms at
dosing levels within the guidance value ranges for
Category 1.
Human:oliguresis, anuria, and nephropathy including renal
tubular necrosis.
LD50 4450mg/kg(Category 5)
LD50 > 10000mg/kg (HSDB)(Category 4)
May cause dermatitis (Category 2)
May cause strong eye irritation (Category 2A)
Affect spermatogenesis (Category 2). May damage fertilityor the unborn child.
This product causes damage to the nervous system,
respiratory organ, kidneys. (Category 1)
This product causes damage to the nervous system,
respiratory organ, kidneys and testis, through prolonged
or repeated exposure. (Category 2)
Not classification

12 Ecological Information
Acute hazards to the aquatic environment

Persistence /Degradability

13 Disposal Considerations

Boric acid: Fish (Rainbow Trout)
LC50=78.1mg(Boron)/L(96hr)(Boric acid equivalent 447mg/L)
Protein and other organic ingredients are biodegradable
and does not remain on the environment for long.

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Product Name : Ascorbate oxidase (ASO-301)

First issue : Oct. 29. 2014
Revised: May. 31. 2022
SDS No. 944F

Safety Data Sheet
Residues
A pollution container and packing

Dispose of in accordance with all applicable local andnational laws and regulations.
Wash with copious amounts of water and waste conforming
to local regulations depending on the type of thematerial.

14 Transport Information
International regulations
IMO information
IATA information
Domestic regulations
Rail and road transportation information
Marine transportation information
Aviation transportation information
Specific precautions transport measures andconditions

Not applicableNot applicableNot applicableNot applicableNot applicable
Avoid direct sunshine and check the container and loading
to prevent leakage or turnover,fall and damage.Transportin accordance with regulations.Do not load with foods andfeed. Keep at temperatures below -20℃.

15 Regulatory Information
Registration, Evaluation, Authorization and Restrictionof Chemicals (EU)Common Chemical nameChemical formulaCAS#
Common Chemical nameChemical formula
CAS#
Boron content of this productA following components of this product are included inSVHC (Candidate list of authorization).
Boric acid
H3BO3
10043-35-3
Sodium tetraborate decahydrate
Na2B4O7・10H2O
1303-96-4
ca. 11 %(W/W)

16 Other Information
Notice

The information shall not be taken as being all inclusive
and is to be used only a guide.All materials and mixtures
may be present unknown hazards and should be used withcaution.The SDS is subject to revision as new information
becomes available.The information in this SDS,to the bestof our knowledge, is accurate and correct. However,TOYOBO
makes no warranty and assumes no liability whatsoever inconnection with any use of this information.