InVi-SPIM

Seeing Upside Down
Very High Imaging Speed
Maximized Photo-eﬃciency
Close-to-Confocal Resolu�on
In Toto Imaging of Small Animal Models
Whole Mouse Embryos, Mammalian Cell CultureLong-term 3D Fluorescence Imaging of Live Specimens

InVi-SPIM
Invi-SPIM system is dedicated to live imaging. It is conﬁgured as an inverted
microscope that has been op�mized for long-term 3 D ﬂuorescence imaging ofliving specimens. Easy access to the sample chamber, maximized photo-eﬃciency,
and short illumina�on �mes enable long-term imaging without harming livespecimens.
The op�cal conﬁgura�on combined with the fast acquisi�on speed of InViSPIM enables 3D reconstruc�on, tracking of cellular and subcellular posi�ons, andmorphological analysis in real �me.

Sample Holder, Strnad et al, Nature Methods 2015

InVi-SPIM system use scanned light sheet technology, with adjustable light sheet
thickness of 2-8 um and ﬂexible sheet area. It also have 2 detec�on modes: Area
mode for fast and high sensi�vity imaging, and Line Scanning Mode forbackground suppression.

Area ModeLine Scanning Mode

The InVi-SPIM provides an unique autoclavable, biologically inert
sample chamber with accurate temperature control and op�onal
environmental control. Sample can be easily accessed for patch
clamp, photoac�va�on and/or photoabla�on.

Fast Imaging
With highly sensi�ve sCMOS
cameras, InVi-SPIM system
can achieve 140 fps @ 512 x
2048 px.

Inverted MicroscopeConﬁgura�on
The system concept provides
easy access to the samplefor moun�ng andTypical applica�ons of InVi-SPIM are in toto imaging of small animal models

manipula�on.

such as whole mouse embryos, imaging of dynamic processes in mammalian cell
culture applica�ons, and even live imaging of intact and living plant models.

Controlled Environment
Autoclavable, biologicallyinert sample chamber,
The cell division processof a mouse to study
Aneuploidy, images taken

accurate temperature control,
op�onal environmentalcontrol.

at -114.5 and -36.5 mins on
both x-y and x-z direc�onby InVi-SPIM system.

Flexible Conﬁgura�on
Customizable laser
combina�on, light sheet
More movies can be viewed in SANE ASIA YouTube channel.

thickness and accessories.

Speciﬁca�onsLaserDetec�on Op�cs

Laser combiner with six laser posi�ons
445, 488, 515, 532, 561, 594, 642, and 685nm @ 50mW
Fast modula�on and high ex�nc�on
Chroma�c correc�on from 440 to 660 nm
Light-sheet genera�on by beam scanning
Variable light-sheet thickness (op�onal)
Water-dipping objec�ve lenses
Fast ﬁlter wheels with 10 posi�ons and 50ms between
adjacent posi�ons
Illumina�on objec�ve: 10x @ 0.3 NA, water immersion

Sample Chamberand StageSystem Control

Detec�on objec�ve: 25x @ 1.1 NA, water immersionHigh-speed sCMOS camera Hamamatsu ORCA-Flash 4.0
Water-sealed inert PEEK plas�c chamber, autoclavable andbiocompa�bleSample supported from below for improved stability
Easy access from above for sample moun�ng etc.
Fast and precise temperature control, range 15-40�C
Environmental Control (op�onal)
3cm long sample holder
Embedded microscope so�ware with open
communica�on interface
Open GUI control for interface control and microscope
automa�on
High-speed RAID controller for data streaming
HIVE High Speed Centralized Data System (Op�onal)h�p://www.saneasia.com