1/5

Product Name : Cholesterol esterase (COE-301)

First issue : Sep. 1, 2014
Revised: May. 31. 2022
SDS No.858F

Safety Data Sheet
1 Chemical Product and Company Identification
Identification of the productProduct CodeSupplier
NameAddress

Cholesterol esterase
COE-301TOYOBO CO.,LTD.
Osaka Umeda Twin Towers South,
1-13-1 Umeda Kita-ku, Osaka 530-0001, Japan

Department

Biotechnology Overseas Sales and Marketing Department

Phone
Fax

+81-6-6348-3846+81-6-6348-3833
diagnostic product

Recommended use and restrictions on use

2 Hazard Identification
Important hazards
Adverse effects on human healthHarmful if Boric acid is swallowed.
Lethal dose (Boric acid) : Adult 10g, Child 5g

GHS classification
Physical hazards
Health hazards
Acute toxicity : Oral
Category 5
Acute toxicity : Dermal
Acute toxicity : Inhalation (Gas,Vapour)
Acute toxicity : Inhalation (Dust,Mist)
Skin corrosion/irritation
Category 2
Serious eye damage/Eye irritation
Category 2
Sensitization : Respiratory
Sensitization : Skin
Germ cell mutagenicity
Carcinogenicity
Toxic to reproduction
Category 1B
Category 1(nervous system,gastrointestinal trace)
Specific target organ toxicity
Category 3(respiratory irritation)(Single exposure)
Category 1(kidneys)
Specific target organ toxicity(Repeated)
Aspiration
hazardEnvironmental hazards
Acute hazards to the aquatic environment
Category 3
Long-term hazards to the aquatic environment Category 3
Hazard to the ozone layer-:Classification not possible
GHS Label Elements
Symbol/Pictograms

Signal word
Hazard statements

DangerHarmful if swallowed.
Cause skin irritation.
Cause serious eye irritation.
May damage fertility or the unborn
hild damage to nervous system, gastrointestinal tract.
CauseCause damage to kidneys through prolonged or repeatedexposure.

2/5

Product Name : Cholesterol esterase (COE-301)

First issue : Sep. 1, 2014
Revised: May. 31. 2022
SDS No.858F

Safety Data Sheet
Precautionary statements

Harmful to aquatic life with long lasting effects.
Prevention Obtain special instructions before use.
Do not handle until all safety precautions have been readand understood.
Wear protective gloves/protective clothing/eye
protection/face protection.
Do not breathe dust/fume.
Do not eat, drink or smoke when using this product.
Wash hands thoroughly after handling.
Avoid release to the environment.
Response Get medical advice/attentions if you feel unwell.
If on skin: Take off immediately all contaminatedclothing and wash it before reuse. Wash with plenty ofwater /shower.
If skin irritation occurs: Get medical advice/attention
If in eye:Rinse cautiously with water for several
minutes. Remove contact lenses, if present and easy todo. Continue rinsing.
If eye irritation persists:Get medical advice /attention.
If exposed or concerned: Get medical advice/attention.
Storage Store locked up.
Store in a well-ventilated place. Keep container tightlyclosed.
Disposal Dispose of contents/container in accordance withlocal/regional/national/international/regulation.

Important symptoms (Boric acid) Symptoms of vomit,diarrhea,collapse,erythema may appear
after 2-3 hours later and die after 3-5days later fromingestion.

3Composition/Information on Ingredients
Substance/Mixture
Chemical Nature
Chemical specificity
Concentration or concentration range
CAS#
Ingredients Contributing to the Hazard
Common Chemical nameConcentration or concentration range
Chemical formulaCAS#
Common Chemical nameConcentration or concentration range
Chemical formulaCAS#4Mixture
Cholesterol esterase
Freeze and drying powder including enzyme
ca 9%(W/W)
9026-35-3
Boric acidca 60% (W/W)
H3BO3
10043-35-3
Polyethylene Glycol-p-octylphenyl Ether (Triton X-100)
ca 4.5% (W/W)
Not specified
9002-93-1

First Aid Measures
Inhalation
Skin Contact
Eye Contact

Ingestion

Remove to fresh air. Consult a physician whenunpleasantness occurs.
Wash off with plenty of water. Consult a physician when
inflammation on the skin occurs.
Rinse cautiously with water for several minutes. Removecontact lenses,if present and easy to do.Continue
rinsing.
Rinse mouth. Swill plentiful amount of water or milk for
immediate vomiting. Consult a physician.

3/5

Product Name : Cholesterol esterase (COE-301)

First issue : Sep. 1, 2014
Revised: May. 31. 2022
SDS No.858F

Safety Data Sheet
5 Fire Fighting Measures
Specific extinguishing methods
Precautions for fire-fighters

Water spray,dry chemical powder,or carbon dioxide etc.
Firefighter should work from the windward side.

6 Accidental Release Measures
Personal Precautions
Environmental Precautions
Methods and materials for containment andcleaning up.

Wear proper protective gear to avoid eye/skin contact andinhalation. Work from the windward side.
High concentrated waste fluid should not be directly
discharged into rivers.
Take up under vacuum using dust collecting filter, wash
residual spill with copious amounts of water. Use cloth,
paper or anything similar to soak up the solution leaking
out of the container.
(Waste water should be treated with activated sludge
or adsorbed with activated carbon etc.)

7 Handling and Storage
Handling
Technical Measures
Precautions

Wear protective gear to avoid eye/skin contact andinhalation.
Do not drop the container to prevent the content poppingout.

StorageTechnical Measures
Incompatible substances and mixtures
Storage Conditions
Packaging Materials

Keep sealed container in freezer.
None specified.
Store under -20℃ to avoid deactivating.
Use the initial container of the product.

8 Exposure Controls/Personal Protection
Engineering controls
Occupational exposure limit,biological limit
ACGIH (2005)
Personal Protective Equipment
Respiratory Protection
Hand Protection
Eye Protection
Skin and Body Protection

Provide shower and eye washing apparatus nearby.
Boric acid
TLV: 5mg/m3(TWA)

Protecting maskProtecting gloves
Safety goggles
Long sleeve working wear

9 Physical and Chemical Properties
Physical State, form and colorOdour
pH
Flash Point
Explosiveness
DensitySolubility

Powder / Light Brown
No odourca. pH7.0 (1% W/V)
No information available.
No information available.
No information available.
Freely soluble in water.

10 Stability and Reactivity
Stable at temperatures below -20℃.When left for long at
Possible hazard reactions at room temperature,proteins might be degraded, which does
not cause any hazardous reaction.
specific condition
StabilityConditions to avoid

Prolonged storage under higher temperature than room
temperature and high humidity.

Materials to avoid
Hazardous Decomposition Products

No information available.
May react with strong oxidizing compound.

4/5

Product Name : Cholesterol esterase (COE-301)

First issue : Sep. 1, 2014
Revised: May. 31. 2022
SDS No.858F

Safety Data Sheet
11 Toxicological Information(1) TritonX-100
Acute Toxicity (Oral)
Serious eye damage/irritation(2) Boric acid
Acute Toxicity (Oral)
Skin corrosion/irritation
Serious eye damage/irritation
Sensitization
Germ cell mutagenicity

LD50 1700mg/kg(Rat)
May cause serious eye irritation.
Rat LD50 2660mg/kg
Moderate irritation (guinea pig, 24hr, 72hr)
Rebefaction /Pain
No data available
Absence of data on multi-generation mutagenicity tests
and germ cell mutagenicity tests in vivo, and negative
data on somatic cell mutagenicity tests in vivo
(micronucleus tests)

Carcinogenicity
Toxic to reproduction

ACGIH (2005): Category A4Adverse effects on reproduction of parental animals anddevelopment of pups at doses producing no parental
toxicity.

Specific target organ toxicity(Single exposure)

Human:Gastrointestinal tract effects such as nausea,vomiting, abdominal pain and diarrhea, and central nerve
effects such as lethargy, headaches, fever, increased
irritability and muscle convulsion.
Irritation of the upper respiratory tract.
Animal:cyanosis, tetany, spasm and shock-like symptoms at
dosing levels within the guidance value ranges for
Category 1.

Specific target organ toxicity(Repeated exposure)

Human:oliguresis, anuria, and nephropathy including renal
tubular necrosis.

12 Ecological Information
Acute hazards to the aquatic environment
Persistence /Degradability

Polyethylene Glycol-p-octylphenyl Ether (Triton X-100)
LC50/96hr = 3mg/L (bluegill)
This product is biodegradable and does not remain on theenvironment for long.
22% by BOD (National Institute ofTechnology and Evaluation, Japan)

13 Disposal Considerations
Residues

Dispose of in accordance with all applicable local andnational laws and regulations.

Contaminated packaging

Wash with copious amounts of water and waste conforming
to local regulations depending on the type of thematerial.

14 Transport Information
International regulations
IMO information
IATA information
Domestic regulations
Rail and road transportation information
Marine transportation information
Aviation transportation information
Specific precautions transport measures andconditions

Not applicableNot applicableNot applicableNot applicableNot applicable
Avoid direct sunshine and check the container and loading
to prevent leakage or turnover,fall and damage.Transport
in accordance with regulations.Do not load with foods andfeed. Keep at temperatures below -20℃.

5/5

Product Name : Cholesterol esterase (COE-301)

First issue : Sep. 1, 2014
Revised: May. 31. 2022
SDS No.858F

Safety Data Sheet
15 Regulatory Information
Registration, Evaluation, Authorization andRestriction of Chemicals (EU)Common Chemical nameConcentration or concentration range
Chemical formula
CAS#Common Chemical nameConcentration or concentration range
Chemical formula
CAS#Regulations

A following component of this product is put on a listof Substances of Very High Concern (SVHC).
Boric acidca. 47% (W/W)
H3BO3
10043-35-3
Polyethylene Glycol-p-octylphenyl Ether (Triton X-100)
ca. 4.5% (W/W)
Not specified
9002-93-1
Follow all of laws and regulations in your country.

16 Other Information
Notice

The information shall not be taken as being all inclusive
and is to be used only a guide.All materials and mixtures
may be present unknown hazards and should be used withcaution.The SDS is subject to revision as new information
becomes available.The information in this SDS,to the bestof our knowledge, is accurate and correct. However,TOYOBO
makes no warranty and assumes no liability whatsoever inconnection with any use of this information.