yt

HQj,$H?06<0)
Studies on the light and heat stresses on photosynthesis of treesb^Y[[ Ml w \y
School of Pharmacy, Shujitsu University, Assistant Professor
Hiroki MorinT|qN!

ytx

HQjaYf,BC!SZ,H06<0r*))x!

W!*s|06<0L+)m'U06<0A

x

HQjF)

#x!*ah), HN[  z" ^!X

~uDAHQj{i HN[ ¡ }] 9.<>2.E, * W

v X^! He|p!

^_ImC!ytyt:5;^HN[  {i``Vd*)K # ^_I

eH&|06<0AHN[ ¢^!

{i`*)Rp OhCgQ3>7/  K#!J)K kG,PK!c8=4-@1 B)%o(

Abstract
Global warming has a significant impact on the photosynthetic activity of plants includingtrees. During daytime, plants are exposed to stress from strong light in all seasons. In summer,
heat stress is superimposed over the light stress. In the present study, we investigated the effectsof these environmental stresses on photosynthesis, with particular emphasis on the effects of thoseon Photosystem II. Two kinds of experiment were carried out. First is an in vivo experiment
where Photosystem II activity in the leaves of trees was monitored. Second is an in vivo
experiment dealing with degradation of the reaction center-binding protein D1 of Photosystem II inspinach chloroplasts. The former experiment showed that at daytime, in particular in summer, thePhotosystem II activity in tree leaves was inhibited significantly by strong illumination. The invitro study showed that strong light and heat induced cleavage and/or aggregation of the D1 protein,
and a metallo-protease FtsH was suggested to be involved in the D1 cleavage.

-1-ìîêé

ã=¤òă  >¸LĂA4!Ý¥? ¶>ċ zóèæÙµėē¢|ĈÙµĘÝ¥äÜÂĔ?­-:=Û/8#I!ÐÎsÔÈ?ÜÂU[9"IyNt]ÙµÝ¥ċÞóL0I>9¥äÜÂLĀ

Ú0IHÊ:/8Ç±.J8#I(Đ=?

ÔÈLC8Ì

kqb_rhXă>ăčÍÝ&F@_rhXă>ĀEđL/GAI  Í<;Lý64!

¦ *>Ñâ(äÜÂ=FIá[cp[=Ã.J8&H>´x³õ_rhX¨Ç±ĆH>ÏLè¹Ì9)I';$'çÿ.J8#I!

Éìî?

úàË{v9ÑâėÔÈĘ(;>Ó<sá[cp[L+µ(;>Ó<Lí0'$<å[cp[v9$¤ò
í0>'

¶Ù

B43>F

¶>ÙµL·(;>F$<¢é´ðL

L¿G'=0Iêé9ý64!

ìîôć

ĚęČ9>ÔÈ>¶Ùµ>Ý¥!X`eZ

>û>XqqiOoü>Ý¥Lý68#I!

 ìî?ě7>½×9ý64!u7?¡¤Vmrh[>ÔÈė~é=?X`eZìî¶ÏNSVĘ:ª©«>ÔÈėjdQ

]rT\nĘ>û>¤òqqiOoüÝ¥þ÷gQÙµL X

 Č¦ĕ=FI¤ò ė   Ę=FH£ñ-:>å[cp[ā=Ý¥/4ėĚĘ!3>Đ Ě¾>Æ'G ÇėĜÅĘ:£ėĚěÅsĚÅĘ=ÁB9ĚÀĎ&)=Ý¥Lý#ÔÈ>û>¶Ùµė¤ò>Ü®

ÖÜû°®D"K28Ý¥/4!,>

Ý¥=FH

ÔÈ>¶(úà>sÜÙµĘLÝ¥/4!#1J>£ñ=&#8DX

qqiOoü9Ý¥/4¤òÙ®Ë{v9í0Ùµ>Lí0,:(9µė ĝ¶Lý)I!ã=>Òï=ÖÜ>Ė#Ë{9>%IøĘ?

¶Ùµ>(Øêß9"I!

°®(0=7J8

 ìî>y7ê?

¦ĕ§9lQpr^/4ėěĘ!°®>Ė#ÀĎ¬=? Q'Gûö~`nYPdùLĒ/8ý<L/4û>ûö~?¡Ċ>Rfo64!`nYPdù=ĖÜ[cp[ė>WtLá:/8»¼0I(ª©ëĊ½>ÄĖÖÜ=ą# Lĉò

M5ĘLw%L¯)Ą,0!3>À¶ē¢|Ĉ(ď¤ò>_rhXă
-2-x?Ė*Õ²=Ùµ?¡)*}v

uĊ?¤=cnak.J8¤ò>º,ED95 +EGtô6H5#D!

9Y_SIbO's#4#D×¤'Ï

/9,E0!R\MJVØG   5yÆ.

ª87D6x<t¦×{:d«.D'9ú¿²k5:+9×{9D6Y_SIbO'Á},E0!¡'ëED+6'z&30!³

Å

9§n5  z´-0Ó¶  '¿­}'êÛ-º¿'d«-Ô*D6Á},E4#D+6'z&30!,B8#tÂ5ô,E0t¦×{9¡'ëE0C   äïz´8AC  %A<"D#:v)¡-7&30C

9'Îâ,E0ûü!>0 .D×¤'"D!+9A$7h¥87E

yÆd¾6R\MJVGÉ#0x¸¦ù;@Û30!jd9ù8AC¹°9ÈÖ:ô,Evn6-4·

ÃNU^Nµ9ÝçG¨)+687D1F$!

e59t}Ò   Q`WLä9zß8:ðY_SIbO  'óf-4#D

+6'Ï,E0!

  








    µ! " 











0123+*+++,+-+.

+/+0+1+2)'&%$           #  (# ĀýiñÞ»¤a  o¤Y_SIbO  9
¸é[T]! :ØGÿãè.DøG  ±Ñ+3

8ÿý¯9XVK9Ù9t¦×{! d¿6Ù9Üö¿!eàílG±Ñ8@2

āïn¸éG6D!

āïn9g8ò#0Ð8ä5"D©r:º:t6t¦×{! Q`

WLäG(uE4zß.D!

 ùw59t}Ò  9ÇNU^

 9ÃyÆ:cÌ~í99®úº¿8å#¿5"D!¹°9÷

Náp
Ñí8å#Ù9Üö¿:+9¬ 8ì

 ZK^`PK9R\MJVØ8  .D¬@"D!+9A$7¿²k5z9ÃyÆGÛ$6

9ÙÕn9g5:t}Ò  9£g¢

 Q`WLä'ÄÊË8zß,E0!+9Q`WLäzß:iÓQ`WLä'zß,E

ñ9½|5Ú-)qê,E

9Ú-#me'?BED!+9+6:|5@qê,E095>0  9½5æ=0îù5iña  o¤-3-Ùt¦9¼¤
þõÍÒ5À-06¥æ6sÌx6oh2fÎ(&84@4{³6NHIeTd4317

6¹ÞE;$Ù¤1#Bí¶ªm1

^Z`E_Ta2(0ÁÄ6éjz"s",B2'@5|(NUbNª@C0B£¨657YWKJm"szÇ

6  QdXLß5i@TW`436ÒÓÓ°"ì6©¢2CB&254As7?AÔ($è(0â!=(C4&C@6°§7

'CB&254B&6?4ÁÄ5?.

º¶¦z5n.0+6wE²Å50º¶¦z"°§E7)<2*B¯·0B2Û®É¬"Â'C0B"tÐ5(0iBêE¯·6rpb&C@6°§6s"s>í¶13[a-%14$ÈËh6wb[a1¤6?4êE}%B6!Ek6ÁÄ

@!5*B&2"~Ì54.,

1"]HbdPH1Â(,?5upÀ5wb[a1Â(0$Ö"B°§6Ó6ÓÊpszÇ"¼NU

bN5Æ(036?4yEÂ*6!
q8  QdXLß7]HbdPH1×@C,?5¸¾À5wØ'CB6!
 "+6wØ5çDB6!7Ï´ë1B 6lÝà"szÇ6±Ì5#$çDB&2"*15D!

.0B61&6QdXLß6wØáÃ7°§6½52.0=#4E/áÃ1B¿ÕÜ¡ïî]HbdPH6R`MGVÍ6  ZcSF
eO6  ßãw« 7R`MGV?

9  v»gµ6   2ä­Ñ 7 N\LUaw«TeQk6Úë2å °§6s5*Bs>í¶6êEwb[a1Û:B57wb[a6ÁÄ5â4_Ta¯·E'4%C-4-M. Yoshioka, S. Uchida, H. Mori, K.
Komayama, S. Ohira, N. Morita, T. Nakanishi
and Y. Yamamoto: Quality control ofPhotosystem II: cleavage of reaction center D1
protein in spinach thylakoids by FtsH protease
under moderate heat stress. J. Biol. Chem. 281,
21660-21669 (2006)