JCB-SGT DX
An anaerobic protein crystallization
containerFeatures◆ Anaerobic conditions: Crystallization experiments can be performed underanaerobic conditions.
◆ Onboard space experiments: JCB-SGT DX has been used in the protein

crystallization project of JAXA. 3 cylindrical vessels are on a 35 mm widesheet.
◆ Various crystallization methods: Various crystallization methods, such asbatch, vapor diffusion, counter diffusion, dialysis, and diffusion pair -osmotic
concentration can be performed. It is useful for the optimization of theconditions for space experiments and for ground-based control experiments.
The product uses the◆ Soft material: One side of the JCB-SGT DX sheet is made of a film withresults of technologicaloxygen absorption capabilities. The other side is a PET sheet. By crushingdevelopment by JAXA

the silicon tube portion of the tubular sample container inside the JCB-SGT

under license.

DX, the diffusion can be suppressed. With releasing, crystallization can bestarted at the same timing as in space experiments.
◆ Heat seal: After filling the sample, it is heat-sealed. This prevents leakageof liquid completely and ensures high reproducibility and reliability. Since thematerial is low gas permeability, water vapor permeation from inside thecontainer is minimized and the crystals are stable for more than one year.

Cat. ID

Product Name

Qty.

Price(w/o VAT)CRT460JCB-SGT DX

2¥33,200-

JCB-SGT LoadingTools
Point Sealer

Heat Controller Transformer

Cat. ID

Product Name

Qty.

Price(w/o VAT)

CRT300JCB-SGT Loading Tools(Point Sealer & HeatController)

1¥33,000-

CRT301

Transformer for 220V

1

Please contact us※ Please inquire for prices of space-standard products.

Available crystallization methods
Counter diffusion (CD): A capillary is filled with protein
solution, a gel tube (CRT231-6) is attached and theother end is sealed with sealing compound. The capillary
is immersed in a crystallization reagent solution 1). A
single capillary can screen a wide range ofcrystallization concentration conditions 2).
Dialysis (DL): After the sample loading, a gel tube(CRT901-RC-6) with dialysis membrane is attached.
Others are the same as CD1).
Diffusion pair-osmotic concentration (DPOC): Fill aDPOC vessel (CRT850SP-6) with protein sample
solution and crystallization solution successively1).

SealingcompoundCapillary

Proteinsolution

Gel tube

Crystallizationsolution1) Takahashi S, et.al. Int. J. Microgravity Sci. Appl. (2019). 36(1), 360107.
https://doi.org/10.15011//jasma.36.360107.
2) Garcia-Ruiz, J.M. & Moreno, A.: Acta Cryst., D50, (1994) 484-490.
https://doi.org/10.1107/S0907444993014350.
To simulate the space experiment, C-Cap (CRT-413), gel tube with waterproof cover(CRT213-P-6), and DPOC vessel (CRT-850SP-6) are necessary.
For the optimization of crystallization conditions, C-Kit Ground Pro XRD (CRT101-1)
is available as an experimental kit for CD and DPOC methods.

JCB-SGT DX Set up procedure (CD method)
1. Fill each cell of the JCB-SGT DX with about 700 μL
crystallization solution up to around ①. (It is easier
to use the tool made with a glass capillary
connected to the tip of the pipettor tip via a silicontube.)
2. Prepare the capillary assembly with the sample.
3. Load the assemblies into the cells of the JCB-SGT
DX. Normally, the gel tube side should be down.
4. Add crystallization reagent to 1 mm below ②.
5. Heat-seal the upper side of ② while slightly
overflowing the crystallization solution. Be careful
not to leave any air in each cell.
6. Place in an incubator to crystallize.

②①※ JCB-SGT DX uses a sheet containing cerium oxide-based oxygen absorber.
When JCB-SGT DX is used alone, the oxygen absorbing retention period is morethan one month. To maintain the deoxygenated state for a longer period oftime, it is recommended to store the JCB-SGT DX in the deoxygenated storagebag (CRT630).
※ Please inquire details on the setup method for space experiments.

Confocal Science Inc.
Musashino Bld. 2F Fukasawa 5-14-15,
Setagaya-ku Tokyo, 158-0081 Japan
TEL: +81-3-5809-1561 FAX: +81-3-6411-6261
E-mail : info@confsci.co.jp
Web: http://www.confsci.co.jp

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