CONTENTS
1. Welcome speech for the participants to the 6th DKMT Meeting on Ambiental an Environmental
Medicine
The 6th DKMT Meeting on Ambiental and Environmental Medicine – Abstracts
2. The effects of calcium channel blockers on mepivacaine cardiotoxicity in rats
Sanda Mihaela Popescu, M Nechifor, Florica Popescu
3. Markov chains for biological sequence analysis
Anca Kigyosi, C Muntean, Corina Vernic
4. The interaction between epithelium and mesenchyme in pulmonary ontogenesis
Ana Nadia Schmidt, Adriana Muresan
5. The role of ankle-brachial index in diagnosis of peripheral arterial disease
Liliana Gheorghiu
6. A prediction of top athletic performance
P Derevenco, Monica Albu, E Duma
7. The dynamics of the magnesium from serum and from erythrocytes in physical effort
Adriana Muresan, Daniela-Rodica Mitrea, Irina Chis, Viorica Hodor
8. The 80th anniversary of Professor Ion Haulica
M Sabau
1. WELCOME SPEECH FOR THE PARTICIPANTS TO THE 6TH DKMT MEETING ON
AMBIENTAL AN ENVIRONMENTAL MEDICINE
THE 6TH DKMT MEETING ON AMBIENTAL AND ENVIRONMENTAL MEDICINE –ABSTRACTS
We are together at the 6th DKMT meeting on Ambiental and Environmental Medicine, continuing
the tradition established five years ago.
The first DKMT meeting took place in Arad-Romania in 1999, bringing together specialists from
Hungary, Serbia and Romania, and has continued in Szeged – on two occasions – in Petrosani lastyear, in Novi Sad – Serbia and Montenegro.
This meeting is organized by the University of Agricultural and Veterinary Medicine of Banat,
Western University “Vasile Goldis” Arad and the University of Medicine and Pharmacy “Victor
Babes” Timisoara, under the patronage of Timisoara Branch of the Romanian Academy andTimisoara County Council.
The environmental problems related to human health are some of the main problems of the present
and the future and are approached through the interdisciplinary work teams coming from various
specialties related to the environmental and medical sciences.
Besides the traditional topics, like man-made chemicals or natural pollution, human ecology, newterritories will be approached, like medical geology and its applications to human health, andenvironmental damage assessment and remediation.
I am hopeful that this meeting debate modern theories and methods and offer new perspectives oncurrent research in this field, for the benefit and the future sustainability of our civilization.

2. THE EFFECTS OF CALCIUM CHANNEL BLOCKERS ON MEPIVACAINECARDIOTOXICITY IN RATS
Sanda Mihaela Popescu1, M Nechifor2, Florica Popescu1
1
Department of Pharmacology, University of Medicine and Pharmacy, Craiova
2
Department of Pharmacology, University of Medicine and Pharmacy, Iasi
Address for correspondence: Prof. Dr. Mihai Nechifor, Department of Pharmacology, University of Medicine andPharmacy “Gr. T. Popa” Iasi, 16 University Street, 700115 Iasi, Romania. E-mail: nechifor@umfiasi.roABSTRACTThe aim of the study was to determine the effects of calcium channel blockers (verapamil,
nimodipine) on mepivacaine cardio toxicity on rat ECG in vivo. 18 male Wistar rats wererandomized distributed in 3 groups: a control group (mepivacaine) and two study groups which
received a pre-treatment with calcium blockers (verapamil, nimodipine), 15 minutes before
mepivacaine. The association mepivacaine-verapamil significantly shortened PR interval,
insignificantly increased others ECG parameters and produced premature ventricular beats in 25%
rats. The association mepivacaine-nimodipine had a negative chronotropic effect. Nimodipine might
increase mepivacaine cardio toxicity, but it is also possible that some premature ventricular beats toappear as a negative chronotropic effect of nimodipine.
Key words: mepivacaine, interactions, verapamil, nimodipine, heart, rat.

3. MARKOV CHAINS FOR BIOLOGICAL SEQUENCE ANALYSIS
Anca Kigyosi, C Muntean, Corina Vernic
Department of Medical Informatics, University of Medicine and Pharmacy “Victor Babes”
Timisoara
Address for correspondence: Ing. Anca Kigyosi, Department of Medical Informatics, University of Medicine andPharmacy “Victor Babes”, Eftimie Murgu Square No. 2, 300041 Timisoara. E-mail: akigyosi@yahoo.com

ABSTRACT
Many problems in biological sequence analysis have the same structure: based on a sequence ofsymbols from some alphabet, find out what the sequence represents. For proteins, the sequence
consists of symbols from the alphabet of 20 amino acids, and we typically want to know which
protein family a given sequence belongs to. The model of coding DNA is always probabilistic,
allowing computing the probability of DNA sequence, considering that the sequence is coding. In
medical practice, the values (scores) of a given coding statistic in a query sequence can be computed
in a number of different ways.
In this paper we try to present the classical Markov chain of first and high order and its application
for CpG islands.
Key words: Markov chains, transition probabilities, Markov models, DNA sequence, CpG islands.

4. THE INTERACTION BETWEEN EPITHELIUM AND
PULMONARY ONTOGENESIS
Ana Nadia Schmidt1, Adriana Muresan2
1
Department of Anatomy and Embriology
2
Department of Physiology
University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca

MESENCHYME

IN

Address for correspondence: Conf. Dr. Ana-Nadia Schmidt, Department of Anatomy and Embriology, University of
Medicine and Pharmacy “Iuliu Hatieganu” Clinicilor Street No. 1, 400006 Cluj-Napoca. E-mail: anapatol@yahoo.com

ABSTRACTThe lung is a composite of endodermal and mesodermal tissues. The endoderm of the lung bud gives
rise to the mucosal, lining of the bronchi and to the epithelial cells of the alveoli. The vasculature of
the lung and the muscle and cartilage supporting the bronchi are derived from mesoderm. Four stagesof prenatal human lung development (ontogenesis) have been described on the appearance of thelung. There is a close relationship of the blood vessels and the airways during lung development.
Abnormalities in fetal lung development affect both the airways and the blood vessels.
The development (division) of the airways is influenced by growth factors produced in themesenchyme, acting on the receptors in the epithelium.

Key words: pulmonary anomalies, branching morphogenesis, lung growth.

5. THE ROLE OF ANKLE-BRACHIAL INDEX IN DIAGNOSIS OF PERIPHERAL
ARTERIAL DISEASE
Liliana Gheorghiu
Cardiology Clinic, Rehabilitation Hospital, University of Medicine and Pharmacy “Iuliu Hatieganu”
Cluj-Napoca
Address for correspondence: Conf. Dr. Liliana Gheorghiu, Cardiology Clinic, University of Medicine and Pharmacy,
Clinicilor Street No. 3-5, 400006 Cluj-Napoca

ABSTRACT
Peripheral arterial disease (PAD) is a leading cause of morbidity as a result of ambulatory
dysfunction associated with intermittent claudication. Intermittent claudication affects 5% of theolder than 55 years, thereby limiting daily physical activities and negatively affecting quality of life
in many older adults. The population with PAD is at increased risk for cardiovascular morbidity andmortality. This increased risk observed in both patients with claudication symptoms and those whoare asymptomatic. Measurement of the ratio of the ankle to brachial systolic pressure (ABI) is widelyused in clinical and epidemiologic studies to determine the extent of atheroma in the lower limb.
Key words: ration ankle to brachial systolic pressure (ABI), peripheral arterial disease, andatherosclerosis.

6. A PREDICTION OF TOP ATHLETIC PERFORMANCEP Derevenco1, Monica Albu2, E Duma31
Academy of Medical Sciences
2
Institute of Social Research
3”Dragos Voda” University, Cluj-Napoca
ABSTRACTThe limiting factors of the top athletic performance and the psycho-physiological mechanisms
involved remain controversial. The aim of this study was to outline the prediction of the world
records (WR) to 2010 for some athletic track and field events.
The prediction has been performed by means of a computer assisted mathematical model. In short,polynomes that could best approximate WR reached in the last decennials have been asserted overthe period to 2010.
For 100 m dash the forecast is 9.77 seconds for men (M) and 10.09 seconds for women (W). For
1500 m run, these values are 3:22.33 minutes (M) and 3:48.92 (W). For shot put levels are 25.25 m
and 23.32 m, respectively. For high jump the level is 2.54 m (M) and for long jump we advanced
8.39 m (W). The predicted values point to improvement of the WR by a percentage between 10.2 and10.3 depending on event and gender.
Key words: athletic world records, mathematical model, psycho-physiological mechanisms, limiting
factors, doping.

7. THE DYNAMICS OF THE MAGNESIUM FROM SERUMERYTHROCYTES IN PHYSICAL EFFORT
Adriana Muresan1, Daniela-Rodica Mitrea1, Irina Chis1, Viorica Hodor2
1
Department of Physiology
2 st
1 Medical Clinic
University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca

AND

FROM

Address for correspondence: Prof. Dr. Adriana Muresan, Department of Physiology, University of Medicine andPharmacy “Iuliu Hatieganu”, Clinicilor Street No.1, 400006 Cluj-Napoca.

ABSTRACTThe modifications of the extra- and intracellular magnesium are implicated in physiological andpathological processes, some of them with a big importance in clinical practice.
The extremely various physiology of the physical effort involves vascular, respiratory, metabolic andcellular modifications in which the magnesium is drawn up beside the calcium, sodium andpotassium ions.
Magnesium is an antagonistic ion of calcium, an anti-stress ion and a motor vasoadjuster. The
magnesium resources of the organism are more faithfully reflected by the intracellular space, theserum magnesium representing 1% from the magnesium capital of the organism.
This study focused on the distribution of the magnesium in the serum compartment and inerythrocytes (intracellular compartment) in animals – adult male Wistar rats breed, 180-200 g weight– put on the physical effort (swimming with resistance through determinable weights, attached to thesubject, 30 minutes/day, for 3 days). Groups consisting of 12 animals were selected for this study.
The control group comprises 12 rats, Wistar breed, 180-200 g weight, which were not under physicaleffort.
There were measured the main cations from the serum (Mg++, Ca++, Na+, K+) using the Beckman
Cx10 and the magnesium from the erythrocytes was measured from the blood taken on heparin –through the Man Yoe method. It was made evident, from the analysis of the obtained results, asignificantly decrease of the magnesium from the erythrocytes and an increase of serum magnesiumin animals which were put on the physical effort, by comparison with the control group. There were
not made evident the modifications of Ca++, Na+, K+ from the serum by comparison with the control
group. The results of our study are also confirmed by the information from literature, which showsthat the physical effort in non-trained subjects determines the changes in the distribution of extraand intracellular magnesium.
Key words: physical effort, erythrocyte magnesium, serum magnesium, serum cations.
8. THE 80TH ANNIVERSARY OF PROFESSOR ION HAULICA
M SabauProfessor Ion Haulica from the University of Medicine and Pharmacy “Gr. T. Popa” Iasi, member ofthe Romanian Academy, Doctor Honoris Causa, outstanding researcher celebrated on the 29th ofOctober his 80th anniversary.
I had the privilege to meet him at the beginning of 1964 when, being a tutor, I encountered him in the
Laboratory of Experimental Physiology at the “D. Danielopolu” Institute of Normal and Pathological
Physiology in Bucharest, which was led at that time by Gr. Benetato, and I am proud to celebrate him
after four decades.
His entire scientific and pedagogical activity was dedicated to Physiology, that part of medicine
called “the science of life”. Professor Ion Haulica proved to be an extraordinary mind, characterized
by the intuition in approaching the experiments, the eagerness to finish them, by the enthusiasm hetalked about the results he obtained, by the clearness to choose his partners and by the exigency hecherished them, no matter where he worked. Professor Haulica’s great personality was able to fully
manifest in Iasi, the city where he studied medicine, where he began his activity, and from which hissoul never really parted. Generous person, of exemplar rightness, he conducted himself in life by themoral principles of the highest values, in a world less and less willing to respect them. Everything he
did in life was done with great pleasure, being convinced that he likes Physiology, “a hard sciencethat doesn’t offer any material perspective because Physiology doesn’t enrich anybody”, as theformer Ministry of Culture said. He has great heart and communication ability out of limits. No one
has left from him without a good piece of advice, without a word of appreciation meant to help him,
without a smile of encouragement.

Educated in the spirit of Romanian old university traditions, he has a great contribution in training
other generations of good doctors and researchers to continue his work. Actually, Iasi being today thecenter of Romanian Physiology is entirely due to Professor Ion Haulica, who supported the medical
process in Iasi, as a University teacher, Dean or Pro-Rector. He taught everybody in the old fashion,
with great respect and high moral values.
In more than 50 years of scientific activity, he largely contributed to research in the field ofphysiology, approaching a large variety of original physiological themes. He has researches about
vegetative functions, the functional biochemistry of the brain, the rennin-angiotensin system, theneuroendocrine modulation of the stress, the neuroimmune bases of pain and analgesia, thefunctional implications of free radicals and endogenous anti-oxidants, the role of endothelium andthe nitric oxide in vascular reactivity.
A large part of the results of his experimental researches were confirmed and quoted by different
authors in Romania and abroad. Among his well-researchers are: the discovery of rennin-angiotensin
system in hypophysis and pineal gland in mammals and humans, the identification and thedescription of the role of nervous and purinergic fibers from the myenteric plexus, spinal ganglions,spinal cord and other places in central nervous system. He published over 300 papers in national orinternational journals and communicated the results of his work at different scientific manifestations.
He elaborated, as first author, or in collaboration with other scientists, many physiology treaties andmonographs regarding human physiology, some of them awarded by the Romanian Academy. The
volume Human Physiology has already been published in many editions and is the most appreciatedand complete volume which was published in Romania.
Professor Ion Haulica also trained himself in Europe and USA centers where he finalized his
physiological theories and exchang experience with other scientists, and he organized in our country
scientific manifestations with international participation.
He is a member of many scientific organizations from Romania and abroad, he is the vice-presidentof the Academy of Medical Sciences, member of Romanian Academy, Doctor Honoris Causa of the
Universities of Medicine in targu Mures, Constanta and he was the president of the RomanianSociety of Physiological Sciences. He is still a respected member of intellectual community in thecity of Iasi, an active participant at its academic life. Restless and passionate researcher, he continues
his activity with great enthusiasm in the laboratory of Experimental Physiology subordinate to the
Iasi Branch of Romanian Academy.
In the name of the Romanian Society of Physiological Sciences, on behalf of all its members, I wish
Professor Ion Haulica, on this special occasion, to have a long healthy life and to always remain thesame man who dedicated his life to a belief of work and honesty.
Professor Marius Sabau
University of Medicine and Pharmacy Targu Mures
President of the Romanian Society of Physiological Sciences