Honorary President:
Ion Hăulică (Iaşi)SRSF President:
Adriana Mureşan (Cluj-Napoca)
Honorary Presidium:Valeriu Neştianu (Craiova)
Marius Sabău (Tg. Mureş)Francisc Schneider (Arad)
President of the Scientific Committee

Maria Iancău (Craiova)
Scientific Committee:
Carmen Bunu (Timişoara)
Adriana Mureşan (Cluj-Napoca)
Anca Bădărău (Bucureşti)Aurel Nechita (Galaţi)Petrişor Cismaş (Oradea)
Virgil Păunescu (Timişoara)
Dan Dobreanu (Tg. Mureş)Gheorghe Petrescu (Iaşi)Smaranda Goţia (Timişoara)
Manuela Pumnea (Sibiu)
Maria Luiza Flonta (Bucureşti)Aurel Saulea (Chişinău)Maria Grama (Sibiu)
Mariana Rădoi (Braşov)Simona Gusti (Craiova)
Simona Slătineanu (Iaşi)Ileana Ion (Constanţa)Simona Tache (Cluj-Napoca)
Rodica Mateescu (Timişoara)
Maria Vrabete (Craiova)
Georgeta Mihalaş (Timişoara)
Leon Zăgrean (Bucureşti)Daniela Motoc (Arad)
President of the Organizing Committee

Veronica Sfredel (Craiova)
Organizing Committee

Dragoş Ovidiu Alexandru
Daniela Badea
Tudor Adrian Bălşeanu
Florin Burada
Dan Cioroianu
Suzana Dănoiu
Daniel Georgescu
Ionela Iancu
Smaranda Ioana Mitran
Marius Cristian Neamţu
Adrian NeştianuŞtefana Oana Purcaru

Gheorghe RâcăFlorin-Marius Romanescu
Daniela-Elise Tache
Robertina Iulia Tudoraşcu
şi studenţii:Maria Cristina Beznă
Bogdan Cătălin
Mihai Ciorbagiu
Cosmin Cristea
Andrei Deaconu
Adina Riza
Violeta Deaconescu

ABSTRACTSAUTHORS INDEX
1.RED BLOOD CELLS RESPONSE TO HYPERBARIC
HYPEROXIC ENVIRONMENTCECILIA ADUMITRESI, N. CEAMITRU, ILEANA ION, NINELARĂDULESCU, CRISTINA FARCAŞ, CARMEN CIUFU, LOREDANAHANZUPhysiology and Pathophysiology Department, Faculty of Medicine,“Ovidius” University, Constanta
Objectives: The use of respiratory gas mixtures in hyperbaric
conditions during simulated diving, following high oxygen partialpressure in pulmonary alveoli and the increase of the plasma oxygen
concentration, revealed an accident risk, deriving from oxygen toxicity.
Our study aims to measure those parameters that emphasize intricate
effects of hyperbaric environment over the red blood cells of exposedorganisms, induced by its characteristic hyperoxic aggression. Material
and methods: Experiments were performed on Guinea pigs exposed toa pressure of 6 ATA.The breathing mixtures were represented by plain
air and by a mixture of 32% oxygen with 68% nitrogen (Nitrox I).We
assessed the number of red blood cells, their minimal and maximal
osmotic fragility and estimate the percentage of erythrocytes glucose
involved in pentose phosphate pathway, by measuring erythrocytes
Glucose-6-phosphate dehydrogenase activity.
Results:We obtained significant changes of the measured parameters
and noticed specific responces regarding the use of different specificoxygen concentration in the respiratory mixtures. Conclusions: Hyperbarismand hyperoxia induced by the 6 ATA pressure simmulated exposure,using air and nitrox as respiratory mixtures, produce increses of oxygen
radicals production and significant responses of red blood cells number
and their osmotic fragility. The increase of plasma oxygen concentration
following the use of Nitrox as a respiratory mixture (oxygen concentrationin this mixture being 32%) during simulated diving lead to additionalchanges. Erythrocytes Glucose-6-phosphate dehydrogenase is part of
the antioxidant protective system associated to cells ability to neutralize
oxidation stress, in order to avoid their irreversible denaturation, butbeeig itself a possible target.
Key Words: hyperbaric environment, respiratory mixture, Nitrox I,
Glucose-6-phosphate dehydrogenase, reactive oxygen species.

2.SOFTWARE MODELLING METHODS FOR NEUROVISUAL STRUCTURES
ALEXANDRU D., ALEXANDRU LIVIA., GEORGESCU D.,
IANCAU MARIAUMF Craiova
Our only source of visual percepts are the spikes generated by
the ganglion cells, so its important to understand how these potentials
generate visual perception. The main goal is the development of acomputational model of the retina, phisyologically realistic, providing
at the same time a flexible architecture for the structure. The retina
performs data processing, diminishing the number of sent signals from108 photoreceptors to ~106 (the number of fibers the optic nerve contains),
thus being possible to discover new methods for data compression. A
simplified retina model consists of 3 layers of elements that processvisual informations, each of them being connected to the preceding2layers (photoreceptors, bipolar cells, ganglion cells form a path withanterograde transmission).This structure is very similar to a feed forward
neural network. There are cells that have lateral links inside a layer,(horizontal cells, amacrine cells). The presence of these inhibitory or
excitatory layers means the use of image substraction or addition; theuse of operands can be achieved by using concepts from fuzzy neuralnetworks. The image processing tasks are executed by layers offunctionally identical cells and by means of different transition operations
between layers. Temporal processing consists mainly in diminishing theintensity of the stimulus and in delaying the information when it passes
from one layer to another. This model shows the spatio-temporal reply
pf the retina as a whole, as well as the reply of individual cells from thedifferent layers of th eretina. This network has to accomplish this twotasks in a convincing manner.
Key Words: retina, softwrae model, neural network

3.OXIDATIV STRESS AND HOMOCYSTEINE IN
PACIENTES WITH RENAL TRANSPLANT ORSUBMITED TO HEMODIALYSISANTONESCU ANGELA1, MUREŞAN MARIANA1, MICLE OTILIA1,MICLE LIANA1, DOBJANSCHI LUCIANA1, PIRTE ADRIANA1,
DOROFTEIU MIRCEA2, ANTONESCU ANDREEA1
University of Oradea, Medicine and Pharmacy Faculty,Oradea
Romania2
University of Medicine and Pharmacy, Cluj Napoca Romania

1

Background. Increased total homocysteine is very common in renal
patients. Hyperhomocysteinemia is an additional factor that increases
the risk of vascular diseases in general and in renal patients in particular.
The aim of the paper was to point out the associations between thelevel of homocysteine and the oxidative stress in patients submited
to hemodialysis and renal transplant. Material and method. The study
included 30 patients submited to hemodialysis from the Clinical Hospital
Oradea- Department of Nephrology, and 25 patientes with renal transplant
during five months after the surgery. Before and after the hemodialysis
the concentration of homocysteine (total serum homocysteine wasmeasured using the enzymatic homocysteine assay, cat no. FHER100, on
Hitachi 912 instrument) of MDA (with tiobarbituric acid) and carbonylated
proteins (guanidin method) were assessed. In the same time the level ofceruloplasmin, the main antioxidant factor in the plasma was measured(Ravin method). We also estimated the level of creatinine and urea during
those months.The same investigation we made on the patient with renaltransplant. The concentrations of cholesterol and triglicerides were also
evaluated. All resultes were compared with a control group. Results.
Before and after the hemodialysis the homocysteine concentration was
significantly elevated in the blood of the studied patients. The patients
before hemodialysis had an increased level of MDA in comparison withthe control group. Hemodialysis changed in little measure the level of
MDA (p1). The carbonylated proteins had also a high concentration inpatientes in comparison with the control group(p<0,001).After hemodialysis
no remarkable difference was noticed (p>0,1). The concentration of
ceruloplasmin in the serum of renal patients before hemodialysis was
lower in comparison with the control group (p< 0,05). After hemodialysis
the values were similar to those before the intervention (p> 0,1).We also
assessed serum creatinine, uric acid ,urea and total protein. The levelof serum homocysteine was increased in patients with renal transplant.
In these patients an augmentation MDA was present (p<0,001). The

Fiziologia ‑ Physiology  2009 Supplement)

Official Journal of the Romanian Society of Physiological Sciences
concentration of ceruloplasmin in the serum of these patients was low
comparing to the control group (p> 0,1). In the same time we estimatedthe level of cholesterol, triglycerides which are considered cardiovasculardisease risk factors. Conclusions. Concentrations of homocysteine inrenal patients after hemodialysis was high. The hiperhomocysteinemia
was associated with oxidative stress. The level of MDA in patients withrenal transplant was increased in comparison with the control groupand the concentration of ceruloplasmin was reduced which signs ofan oxidative stress. The patients with renal transplantation has also
hiperhomocysteinemia.
Key words: homocysteine, reactive oxygen species(ROS),
hemodialysis, renal transplant

4.NEW APPROACES IN RECOVERING THERAPYIN MULTIPLE SCLEROSISELENA TAINA AVRAMESCU1, MARIUS CRISTIAN NEAMŢU2, MARIAIANCĂU3, ADRIAN NEŞTIANU4, OANA MARIA NEAMŢU5
University of Craiova, Faculty of physical Education and Sport, Departmentof Human Anatomy
2
University of Medicine and Pharmacy Craiova, Faculty of Midwives
and Medical Assistance, Department of Normal and Pathologic
Physiology
3
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Pathologic Physiology
4
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Normal Physiology
5
University of Craiova, Faculty of physical Education and Sport

1

Freeman et co (1999) made a random study on 66 patients with
progressive multiple sclerosis. The patients were evaluated conform to
Kurtzke disability scale and the target group followed a recovery programof 20 days. The patients` disability and functional independence were
evaluated at the beginning of the study and after 6 weeks and London
scale for handicap was applied. The two groups were classified according
to age, gender, evolutive level and disease duration. The group who
followed the recovery program showed improvements in their disability
degree and participation degree, comparatively with witness group. A
more complex study evaluated the effects of physical training involving
physical exercises and strength exercises on patients with multiple
average and mild sclerosis – Romberg et co, 2004. The trainings took
place in hospital within 3 weeks and at the patients` location within 23
weeks; the results were compared to those of a witness group. All thestudied patients registered improvements of the evaluated parameters.
In some countries patients with MS are treated by electrical stimulation,
meaning the use of some electrodes on the affected muscles, substituting
thus physiological nervous stimulation. The method may be used in thecase of the patients who can still walk. Functional electrical stimulation
as treatment in multiple sclerosis is hardly used due to the lack ofclinical documentation. Since MS is a chronic disease with periodsof stagnation and recurrence, the indications in the use of electricalstimulation vary according to symtoms and functional limits. Electricalstimulation improves muscular force, coordination, balance, walking
ability and the efficiency of routine activities.
Key words: functional electrical stimulation, disability.

Fiziologia ‑ Physiology  2009 Supplement

5.NOVELTIES IN NEUROPHYSIOLOGIC ASPECTS
IN MULTIPLE SCLEROSISELENA TAINA AVRAMESCU1, MARIUS CRISTIAN NEAMŢU2, MARIAIANCĂU3, ADRIAN NEŞTIANU4, OANA MARIA NEAMŢU5
University of Craiova, Faculty of physical Education and Sport, Departmentof Human Anatomy
2
University of Medicine and Pharmacy Craiova, Faculty of Midwives
and Medical Assistance, Department of Normal and Pathologic
Physiology
3
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Normal Physiology
4
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Pathologic Physiology
5
University of Craiova, Faculty of physical Education and Sport1

Neurophysiologic aspects involved in multiple sclerosis refer toelectrophysiologic and molecular substrat responsible for the nervous
conduct modified by demyelinated axons. This modified conduct ofdemyelinated axons is the result of electrical alterations at axonal
level. Many studies show that at the level of Ranvier throttles are great
quantities of Na channels of 1000/µm2 at the level of nodal membrane,comparatively with 25 /µm2 at the level of internodal membrane. The
number of Na channels is almost equal owing to the low surface of nodal
membrane. Immunohistochemical and electrophysiologic studies haveindicated a great density of Na currents The aspect is important since
syncronic activation of these channels generate a sufficient current toactivate the following knot. Myelinated axons have also channels forpothasium. Pothasium axonal currents are present in low quantity at theknot level but they are very frequent within the myelin layer. It has beensuggested that the density of pothasium rapid current is maximum injuxtaparanodal region and reduced to 1/6 in nodal region. This reduced
nodal distribution prevents hyperexcitability. Blocking the conduct may
depend on the frequency of impulses; the conduct takes place in thecase of low frequncy impulses. Blocking the conduct of high frequency
impulses may be the result of hyperplarization caused by the activity ofelectrogenic pump. It has been suggested that an increase of intracellular
Na concentration at nodal level, associated with extracellular accumulationof pothasium in demyelinated areas could lead to inactivation of Na
channels and blocking of nervous impulse conduct.
Key words: nervous conduct, demyelinated axons

6.The corelation of cytogenetic
abnormalities with bone marrowplasma cell infiltration in multiple
myeloma
DANIELA BADEA1, M. BADEA2, CRISTINA PETRICĂ3, AMELIAGENUNCHE-DUMITRESCU4, LUCICA ROŞU5, SMARANDAMITRAN1
Department of Physiology, University of Medicine and Pharmacy,
Craiova, Romania
2
Department of Haematology, University of Medicine and Pharmacy,
Craiova, Romania
3
Department of Physiopathology, University of Medicine and Pharmacy,
Craiova, Romania
4
Department of Internal Medicine, University of Medicine and Pharmacy,

13Official Journal of the Romanian Society of Physiological Sciences
Craiova, Romania
5
Department of Microbiology, University of Medicine and Pharmacy,
Craiova, Romania
Multiple myeloma (MM) is an incurable malignancy. Recent insights
into the biology of MM have provided a framework in the development ofdrugs that target not only intracellular pathways, but also the myelomabone marrow microenvironment interaction. Material and methods. Our
lot contains 44 patients with MM at diagnosis; average age is 63,3±4,44
years; ratio male/female 1,86. 75% of patients are in III stages (staging
system Durie-Salmon). Regarding the type of plasma cell morphology
the lot was shared as follows: 9,09% plasmablastic group, 18,18%
immature group, 15,9% intermediate group and 56,81% mature group(Greipp). The therapy was Alkeran and Prednisone in over 75% of
patients and the others received different polychemotherapy. Results.
The cytogenetical examination was + in 29,54%, most frequent involved
chromosomes 1;14 and cytogenetic abnormalities was complex (structuraland numerical) in 83,3% cases; plasma cell infiltration >30% in 39,28%
cytogenetic abnormalities. The 24 month survival rate was 61,53% for
the patients with cytogenetical abnormalities and 87,1% for the patients
without cytogenetic abnormalities (Fischer exact p=0,09). The 36 monthsurvival rate was 33,07% vs 64,45% for the same groups mentioned
before. Conclusions. The plasmablastic and immature type of morphology
has a negative prognostic impact to the survival of patients with MM.
The presence of cytogenetic abnormalities seams to be correlated withthe plasma cell infiltration degree, the morphological subtypes and withan unfavorable prognostic, too.
Key words: multiple myeloma, cytogenetical abnormalities, plasma
cell infiltration.

7.INCIDENCE AND CONSEQUENCES OF HEMORRHAGE
AND THROMBOSIS IN CHRONIC MYLOPROLIFERATIVEDISEASESDANIELA BADEA1, M. BADEA2, CRISTINA PETRICĂ3, LUCICA
ROŞU4, AMELIA GENUNCHE-DUMITRESCU5, ADRIANBĂLŞEANU1
Department of Physiology, University of Medicine and Pharmacy,
Craiova, Romania
2
Department of Haematology, University of Medicine and Pharmacy,
Craiova, Romania
3
Department of Physiopathology, University of Medicine and Pharmacy,
Craiova, Romania
4
Department of Microbiology, University of Medicine and Pharmacy,
Craiova, Romania
5
Department of Internal Medicine, University of Medicine and Pharmacy,
Craiova, Romania

1

Chronic myeloproliferative diseases are clinical entities that
include clonal disorders of the multipotent stem cell with distinct clinicalcourse and long and sometimes intricate progression. Material andmethods. We evaluated the hemorrhage and thrombosis in a groupof 80 patients: 52,5% with chronic myeloid leukemia (CML), 23,75%
with policitemia vera (PV), 17,5% with essential thrombocytosis (ET)and 6,25% with agnogenic myeloid metaplasia (AMM). Results. Fromthe clinical and laboratory data it was noticed the high frequency ofthrombosis especially in ET 50% and PV 26,31% and the hemorrhage(CML 76,19%, AMM 40%), some of them fatal. The bleeding time was

4

prolonged in 11,9% of cases in CML, in ET 28,57% and in AMM 40%.
The prothrombin time was prolonged in CML 26,19%, in PV 21,05%, in
ET 7,14%, in AMM 40%. The activated partial thromboplastin time wasprolonged especially in AMM 40%, CML 26,19%, PV 21,05%, ET 7,14%.
The clot retraction was deficient in 8,7% of cases. Further more, theantiplatelet therapy and chemotherapy by inducing thrombocytopenia
and pancytopenia respectively increased the risk of hemorrhage (4patients, 5%). Thrombosis does not correlated with the number ofplatelets and apparently a thrombosis of over 1000000/mm3 increased
the risk for hemorrhagic complications. Conclusions. Although theseclinical entities are slowly progressive diseases, the overall survival andprognosis of these patients are significantly marked by hemorrhagic andthrombotic complications, hence the necesity for a carefully monitoringof coagulation status.
Key words: chronic myeloproliferative diseases, hemorrhage,
thrombosis.

8.INFLUENCES OF ALL TRANS RETINOIC ACID
AND ULTRAVIOLET LIGHT EXPOSURE ON NORMALHUMAN MELANOCYTE CULTURESBALDEA IOANA, COSGAREA RODICA, FILIP ADRIANA,
MURESAN ADRIANA”Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca,
Romania
Background: All-trans-retinoic acid (ATRA) is an A vitamin derivate,
which has a complex behavior although it is used as hypo pigmenting
agent. It is able to activate Mitf expression through PKC and stimulatemelanocyte differentiation. However, it also reduces the number of cells,
since it triggers apoptosis. It might increase pigmentation when thelevels of melanin are very low, or melanocytes are scarce and inactive,
but it has opposite effects in melanocytes previously exposed to UV.
Objective: To study normal human melanocytes behavior in cultures
exposed to different concentrations of ATRA associated with UVA andUVB radiation. Methods: Normal human epidermal melanocytes wereincubated with various concentrations of ATRA, (10-9M - 10-5M), in
serum free, basic melanocyte media for different periods of time: 2, 4, 6,
8, 24h, then irradiated with UVB 10-30 mJ/cm2, respective UVA 20-50
mJ/cm2. Proliferation was assessed using a MTT assay; total melanincontent was measured through spectrophotometry against a standard
curve, using synthetic melanin; the tyrosinase enzymatic activity as
DOPAchrom oxidase was measured spectrophotometrically using the
enzymatic activity of mushroom tyrosinase as a control; melanocyte
senescence was assessed by counting the nuclear senescence fociunder fluorescence microscopy observation after H2AX completedwith Alexa fluor antibodies immunostaining. Results: The cultured
melanocytes behavior can be influenced by the small amounts of
ATRA in the culture media that we used, but the relationship between
concentration and effect is not linear, it also depends on the exposure
time. While short periods of exposure to small ATRA concentrations
had a constant stimulating effect on cell proliferation, pigment genesis
and slightly improved senescence, a longer exposure period decreased
total melanin content, although the enzymatic activity of tyrosinaseremained increased compared to the untreated controls, without
affecting cell proliferation. In all the irradiated melanocyte cultures
we observed increasing of pigment genesis. However there was animportant difference between the cultures exposed to the ATRA - UVA

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
and ATRA –UVB combination on cell proliferation, melanogenesis andsenescence. While ATRA - UVA irradiation had a stimulating effect onmelanocyte proliferation and decreased cell senescence, ATRA –UVB
decreased cell proliferation at higher doses of light exposure andincreased senescence. Conclusion: In our experiments, ATRA had
different effects on melanocyte survival, proliferation, pigment production
and senescence, according to the concentration used, association with
UV exposure and time elapsed.

9.EFFECTS OF GRANULOCYTE-COLONY STIMULATINGFACTOR AFTER STROKE IN AGED RATS
A. BALSEANU1,2, K. STÖCKER3, K. DIEDERICH3, J. MINNERUP3, C.
MARGARITESCU1,2, W.R. SCHÄBITZ3 A. POPA-WAGNER1,2
Department. of Neurology, University of Greifswald, Germany
2
University of Medicine and Pharmacy Craiova, Romania
3
Department of Neurology, University of Münster, Germany

1

Background and purpose: In aged humans, stroke is a major causeof disability for which no neuroprotective measures are available. G-CSF,
a member of the cytokine family of growth factors, promotes brainneurogenesis and improves functional outcome after stroke in the young
animal. Here, we test the hypothesis that G-CSF provides restorative
therapeutic benefit to the aged animal. Methods: Focal cerebral ischemia
was produced by reversible occlusion of the right middle cerebral arteryin 19-20 month-old male Sprague Dawley rats. One hour after stroke,
the aged rats were treated daily with 15µg/kg G-CSF and for 15 days
in total. Rats were behaviorally tested and the brains analyzed after 28
days post-stroke. Results: G-CSF treatment had a beneficial effect onsurvival rate, functional recovery of motor function (rotarod, inclined plane)
and working memory (radial maze). However, the beneficial effects oftreatment was generally limited to the first 12 days post-stroke. At cellularlevel, the G-CSF treatment increased the number of proliferating cells
in the SVZ and the dentate gyrus and increased the number of newborn neurons in the SVZ, ipsilateral to the lesion. Conclusions: These
results suggests that the G-CSF treatment in aged rats has primarilya beneficial effect on functional outcome most likely via supportive
cellular processes such as neurogenesis. Further studies are required
to optimize G-CSF treatment schedule in aged subjects.

10. THE CIRCADIAN RHYTHM OF THE COAGULATIONFIBRYNOLYSIS BALANCE (CFB): A PHYSIOLOGICALREALITY IN NEWBORN ?
ANCA BĂDĂRĂU, MARIANA ARTINO, RALUCA PAPACOCEA,
CĂTĂLINA CIORNEI, EREMIA IRINAUMF Carol Davila Bucharest
The circadian variations of some aspects of term newborns infants
homeostasy (other than CFB were identified both in newborns andpreterm newborn infants. In the present paper we proposed to follow
up a number of CFB parameters, in different moments of the day : 6-8
a.m. and 6-8 p.m. respectively. Our study was performed on newborns
infants divided in 5 groups depending on gestational age (GA), bodyweight and APGAR score. The obtained results showed significant
statistic differences in the term newborns infants with a normal body

Fiziologia ‑ Physiology  2009 Supplement

weight and APGAR score more than 8,depending on the moment of the
day when the birth took place. In preterm newborn infants born during
evening, the statistic data do not differ from the preterm newborn infants
born during morning.

11.SALIVA ENZYME’S CHANGES IN NEW DIAGNOSEDDIABETES MELLITUS VERSUS ORAL CAVITYDISEASESANCA BĂDĂRĂU, MARIANA ARTINO, RALUCA PAPACOCEA,
CĂTĂLINA CIORNEI GRATIELA MIHAIUMF Carol Davila Bucharest
One of the most often met consequences of the diabetes mellitus,
more than renal injuries, are the oral cavities injuries. The diabetes
mellitus and orofacial diseases involves both external factors and the
local and general body response. In the present paper we proposed tofollow up the aminotransferases (TGO, TGP) and LDH evolutions as
indicators of the tissue destruction and even as indicators of the oxidative
stress in patients with new diabetes mellitus, parodontopathies and oralcavity tumors. In the new diagnosed diabetes mellitus the saliva TGO,TGP and LDH presents significant increases comparative with plasma
values of the same parameters. A change in enzymatic systems was
identified in the group with parodontopathies (low significance, p>0,05).
Low significant changes in the studied enzymes level were identified inpatients with benign and malignant tumors of the oral cavity. (p>0,05).
In the group with many tooth decays significant changes in the TGO,TGP (p<0,001) but not for LDH (p>0,05) for were noticed in comparison
with controls. In the studied dental and orofacial diseases cannot be
described a sustained correlation between their presence and theevolution of the studied enzymes.

12.SERIC AND SALIVARY IONIC CHANGES IN C
VIRUS HEPATITISDANIELA BĂDIŢĂ1, ADINA OLTEANU1, MONICA DRAGOMIR1,
VICTOR NIMIGEAN2
Carol Davila University of Medicine, Dental Medicine Faculty, Bucharest,
Department of Physiology,
Carol Davila University of Medicine, Dental Medicine Faculty, Bucharest
Topografic Anatomy Department
Ionic homeostasis can be affected in hepatic viral infections, byaffecting the hepatic inactivation for some hormones and the acid-basicbalance. We have assessed ionic plasmatic and salivary levels for 35
healthy subjects (control group) and for 34 patients with virus C chronic
hepatitis (CCH). Plasma total calcium, sodium and potassium presented
variations between the normal values for the lab methods and nonsignificant statistically (p > 0.05). Ionic calcium was significant higher(p > 0.05) for the patients with CHH (4.29 ± 0.33 mg/dl) compared tothe control group (3.98 ± 0.35 mg/dl); the same for magnesium: 2.02 ±0.31 mg/dl at CHH group, compared to 1.83 ± 0.29 mg/dl for the control
group. In total non-stimulated saliva, the potassium level was significant
lower (p < 0.01) in CHH (11.48 ± 2.30 mmol/l) compared to the control
group (22.31 ± 8.88 mmol/l). Chloride level was significant higher (p< 0.01) in CHH (63.91 ± 5.88 mmoli/l) compared to the control group5

Official Journal of the Romanian Society of Physiological Sciences(42.20 ± 15.23 mmol/l). Salivary calcium is significant lower at the HCC
group (11.76 ± 0.32 mmol/) compared to 1.95 ± 0.46 mmol/l for control
group. Conclusions: In virus C hepatic infections we did not observe
plasmatic pathological changes for sodium, potassium, total calcium
levels, but ionic calcium and magnesium levels were increased. The
decrease of potassium and calcium levels and the increase of chloride
level in CHH patients’ saliva could suggest the involvement of secretory
and reabsorption processes from salivary striate ductus.

13.LEFT VENTRICLE RESTYLING STUDY IN
ARTERIAL HIGH BLOOD PRESSURE DISEASE FOR
A PATIENTS SAMPLE FROM KOSOVO AREA
BEKIM MALA1, GUSTI SIMONA2, VICTORIA BELU3, DAN
NITA4
Cardiology Department, Dr Daut Mustafa Hospital- Prizren,
KOSOVO
2
Physiology Department, Medicine Faculty, U.M.F. Craiova,
ROMANIA
3
Cardiology Department, Campulung Hospital, ROMANIA
4
Military Hospital, Bucharest, ROMANIA

1

The authors perform a clinical and a paraclinical study for a lot of 30
patients suffering of arterial high blood pressure, stage I (systolic arterialpressure 140-159 mmHg and dyastolic pressure 90-95 mmHg). The
patients were male gender predominant and living in urban environment.
The EKG was performed with NIHON KOHDEN 9020K devices forinvestigation of left ventricle hypertrophy (LWH) using Sokolow-Lyon andCornell traces (signs); Echocardiography was performed with ALOKA
4000 devices for investigation of left ventricle hypertrophy presence orabsence, ventricular mass and sistolic and diastolic function. The goalof the study was to measure the above parameters for new investigatedpatients and for old investigated patients also (patients with disease 2-5
years old). From EKG and Echocardiography analysis of investigatedpatients we noticed that we do not find left ventricle hypertrophy (LWH)
for 20 patients (66.6 % from examined group). For 10 patients(33.3%from examined group) we noticed left ventricle hypertrophy (LWH) as
follow: concentric type hypertrophy for 9 patients (30 %) and eccentric
type hypertrophy for 1 patients (3.33 %). We note that left ventricle
restyling and systolic function alteration begin in early stage of High
Blood Pressure stage I (administrating a specific treatment) and it isimportant for patients with 3-5 years old disease.
Key words: Arterial High Blood Pressure stage I; left ventricle
hypertrophy; Electrocardiogram, Echocardiography.

14.STUDY OF CEREBRAL BIOELECTROGENESISIN CHRONIC HEPATIC DISEASESMARINELA BEZNA, S. BEZNA, A. NESTIANU, MARIA IANCAU,
D.GEORGESCU, MARIA CRISTINA BEZNA, P. BADEA, V.
NESTIANU
University of Medicine and Pharmacie of Craiova
The electrical recorder of cerebral bioactivity is important in theassessment of patients with hepatic-cerebral syndrome. Aim: Presenting
some cerebral electrophisiological findings which can delimitate a diagnosis

6variation profile in chronic hepatic patients. Patients and methods:
The study was made on a group of 220 patients with chronic hepaticdiseases, to whom were recorded visual evoked potentials (VEP) andelectroencephalography (EEG). Results: Electroencephalography andvisual evoked potentials permit a neuroelectrophysiological status ofhepatic patients and a modified aspect can have significance and measure
the degree of perturbations. The aspect of modifications in VEP waves
parameters were elongation of latency and duration (121%), diminutionof amplitudes (55%), abruptness (52%) and surfaces (48%) and theincrease of the differences in cerebral answer between eyes (480%).
EEG is hypo volted or plate (α amplitude-36 µV); rich in slow waves (δand θ, 85%) and the presence of triphasic waves (1 patient). Conclusions:
Perturbations in spontanous or evoked electrical cerebral activity shows
an electrophysiological profile in cerebral suffering in hepatic patient.
The suggesting aspects are with decrease energy of cerebral waves in
1/3 of patients with chronic hepatic disease. The electrophysiologicalstatus with prolonged latencies and timing, decrease of amplitude andsharpness of VEP waves and especially the great differences of latency,
timing and amplitude between eyes, as well as the appearance of slow
waves (θ and δ) and EEG hypo voltage, demonstrate the cerebral
aggresion in hepatic disease and its treatment.
Key woords: chronic hepatic diseases, VEP, EEG

15.OPTIMAL CONTROL FOR BLOOD GLUCOSEWITH REFERENCE MODEL
Cristina Beznă, Ileana NEACĂ, IONELA IANCU
University of Medicine and Pharmacy of Craiova, Faculty of Medicine
The realisation of the artificial pancreas is presently a fundamental
high priority research area with a strong interdisciplinary character(physiology, diabetology, automated systems engineering). The objective
consisted in the building of a mathematical model of the blood glucose
control system as correctly as possible and the development on its
base of evolved control algorithms in an automated state, adapted to
the real-life situations encountered in medical practice. The realisationof these algorithms constitutes an important stage in the constructionof insulin pumps with automated control (closed loop) similar to thephysiological pancreas. Model Predictive Control (MPC) is a multivariable
control algorithm that uses: an internal dynamic model of the process(blood glucose control system); a history of past control moves; anoptimization cost function over the receding prediction horizon, tocalculate the optimum control commands. The output of the system
is compared to a desired response from a reference model. By doingthese, we are strongly reducing the influence of external perturbations(meals, physical exercise, stress etc.) as well as illness.
Key words: blood glucose control, optimal control, mathematicalmodel, artificial pancreas

16.RELATIONSHIP BETWEEN THYROID FUNCTION
AND COMPONENTS OF THE METABOLIC SYNDROME
IN HEALTHY SUBJECTSBOBCOVA SVETLANA1, PROTOPOP SVETLANA1, LYSYILEONID1, SAULEA AUREL2, DRAGAN BORIS2
Department of Biochemistry and Clinical Biochemistry, State Universityof Medicine and Pharmacy “Nicolae Testemiţanu”, Chişinău, Moldova
2
Department of Human Physiology, State University of Medicine and1

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
Pharmacy “Nicolae Testemiţanu”, Chişinău, Moldova
Objective: We aimed to investigate the relationship between thyroid
function and components of the metabolic syndrome in euthyroid subjects.
Design and methods: In thirty healthy women it was determined thyroid
stimulating hormone (TSH), free triiodthyronine and free thyroxine levels,
lipid and lipoprotein concentrations, fasting glucose and insulin levels,
homeostasis model assessment of insulin resistance (HOMA-IR), it wasmeasured waist circumference, body mass index (BMI), systolic anddiastolic blood pressure. The patients were divided into two groups infunction of TSH levels (fifteen patients with TSH levels < 1,46 mIU/L(1,13±0,23 mIU/L) and fifteen patients with TSH levels ≥ 1,46 mIU/L(1,99±0,59 mIU/L), p<0,0001). Results: Investigated indices did not differ
between the two groups, except high-density lipoprotein cholesterollevels (1,67±0,48 mmol/L vs. 1,31±0,36 mmol/L, p0,03) and waist
circumference (74,2±14,5 vs. 80,5±10,1 cm, p0,05). TSH positively
correlated with BMI (b0,31, p0,016), waist circumference (b0,31, p0,017),
and negatively correlated with high-density lipoprotein cholesterol levels(b-0,35, p0,007). BMI was positively associated with systolic bloodpressure (b0,47, p0,0009), diastolic blood pressure (b0,39, p0,006),
and negatively associated with high-density lipoprotein cholesterollevels (b-0,31, p0,018). Waist circumference was associated positively
with systolic blood pressure (b0,45, p0,0016), diastolic blood pressure(b0,36, p0,013), triglyceride levels (b0,28, p0,035), and negatively
associated with high-density lipoprotein cholesterol levels (b-0,33,
p0,012). Conclusion: We have demonstrated an association between
TSH levels within the normal reference range and some components ofthe metabolic syndrome. These findings are consistent with an increased
metabolic risk in subjects with low normal thyroid function.
Key words: thyroid function, metabolic syndrome, body massindex.

17.HUMAN MESENCHYMAL STEM CELLS TRANSITIONTOWARD THE EPITHELIAL LINEAGE – IN VITROSTUDY
BOJIN MF, SISKA IR, HERMAN D, BUNU C, TATU C, ANGHEL
S, DEAK E, TATU CA, TANASIE G, PAUNESCU V
University of Medicine and Pharmacy Victor Babes, Timisoara
Background and aims: Several studies showed that mesenchymal
stem cells (MSCs) can give rise, in vivo, to epithelial cell types inlungs and other tissues. The aim of this study was to examine thein vitro differentiation potential of human MSCs toward the epithelial
lineage. Material and Methods: For isolation and culture of human
MSCs we used the bone marrow aspirates obtained from the sternumof 8 patients with different hematological disorders. Informed consent
was signed by each patient. Plastic adherence and culture in standard
medium provided a good isolation and purity of MSCs. After the second
passage, MSCs were plated in uncoated flasks using the same medium,
gradually supplemented with specific growth factors. Expression ofcytokeratin 19 and 18 was assessed using immunocytokemistry after
7-14 days from the initial plating. Relative quantification of epithelial
marker cytokeratin 18 mRNA was performed using real-time RT-PCR.
Results: The epithelial-like cells acquired a rounded/polygonal shapeand formed an adherent monolayer, organized in cobblestone patternclusters. These cells were positive for CK 19 (40% of total cells). During
the epithelial differentiation of the MSCs, the level of some specific markers

Fiziologia ‑ Physiology  2009 Supplement

determined by flowcytometric analysis was decreased. The RT-PCR
detected strong expression of CK18 in the cultured cells. Conclusion:
This study provides preliminary results indicating that MSCs can acquire
an epithelial phenotype when submitted to appropriate differentiation
protocol, thus being possible candidate for further genetic engineeringwith medical or research purpose.
Key words: mesenchymal stem cells, epithelial lineage,
cytokeratin

18.CLINIC AND PARACLINIC STUDY OF A GROUP
OF CHILDREN WITH BEDWETTING FROM DOLJAIDA BONTEA1, SIMONA GUSTI2, V. IOVANEL1
1

Segarcea City Hospital
University of Medicine and Pharmacy of Craiova

2

The bedwetting is defined such involuntary pee (night/day) of achild over 4 years (limit arbitrary considered to be maximum age until
the healthy child must obtain sfincterian control, including night control).
Bedwetting suppose the persistence of involuntary nocturnal pee formore of two times in a month to a child over five years. From a groupof 476 of children, with age between 5-12 years, predominant boys andfrom country hospitalized in Craiova Child Neuropsychiatry Hospitalfor different disorders such as epilepsy, comportamental problems, it
was put the diagnosis of bedwetting to a group of 30 children. This
group had included children with ages between five to twelve years,
predominant boys (80%), and from country (77%). I had studied thisgroup using detailed body examination, psychiatry exams, urinary tests,
urinary culture, lombosacrat radiography, electroencephalography,
computer tomography. I had identified the existence of urinary infections
to 3 children (10%), spina bifida to 6 children (20%), and from these
predominant to the female (83%). Psychiatry exams put the diagnosis
of delay in phisiatrical development with IQ under normal to 8 children(26%), predominant boys. In the study group, 77% (23 patients), hadpresented on electroencephalography iritiatives lines, and the rest ofchildren (23%), epilepsy –7 patients, predominant female. After a cranialtraumatism to 3 children who in time presented bedwetting, the computer
tomography wasn’t revealing pathological aspects.

19.EVALUATION OF THE ELECTROPHYSIOLOGICAL
CHARACTERISTICS IN POLYNEUROPATHIESCLAUDIA BORZA1, RODICA MATEESCU2, GERMAINESAVOIU1
1
Department of Pathophysiology, University of Medicine and Pharmacy
Victor Babes Timişoara
2
Department of Physiology, University of Medicine and Pharmacy Victor
Babes Timişoara

The aim of this study was to evaluate the utility of electromyography
and nerve conduction parametres in evolution of the polyneuropathies. The
patients were classified depend on the site of the lesions, the clinical signs
and the initial stage of the disease. The electrophysiological characteristics
were analysed: the amplitude of the muscle action potential, the proximaland distal latency and for electromyography: the amplitude, duration,
frequency and the aspect of motor unit potentials. Results: the motor deficit

7Official Journal of the Romanian Society of Physiological Sciences
and the decrease of muscular force were the main clinical signs in these
diseases. In these patients an electromyographic neurogen recording
type was found and a decreased value of motor conduction velocity forcorresponding nerve was observed. Conclusion: The electromyographic
neurogen recording type is characterized by a spontaneous activity inresting state and during contraction a low recrutation pattern and highamplitude and duration of the potentials were record. These parametres
together with the clinical signs established the positive diagnosis of thepolyneuropathy and the prognostic of the disease.
Key-words: polyneuropathy, electromyography, motor nerve
conduction velocity

20.THE PREVALENCE OF METABOLIC SYNDROME
IN PATIENTS WITH ARTERIAL HYPERTENSIONCLAUDIA BORZA1, RODICA MATEESCU2, GABRIELA NEGRISANU3,
CORINA SERBAN1, LELIA SUSAN4
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of Pathophysiology
2
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of Physiology
3
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of 3rd Internal Medicine
4
University of Medicine and Pharmacy “Victor Babes”, Timisoara, The
IVth Medical Clinic

1

Aim: Metabolic syndrome is strongly predictive of future diabetes
and its presence in hypertensive patients who may be at risk for diabetes
should be investigated. The purpose of this study was to determine theprevalence of metabolic syndrome in a group of hypertensive patientsusing the criteria of the Cholesterol Education Program’s Adult Treatment
Panel III. Material and methods: A population of 102 hypertensive
patients (46 males and 56 females) over the age of 40 were screened
for metabolic syndrome by determining body mass index (BMI), waist
circumference, levels of fasting plasma glucose and fasting plasma
lipids (serum triglycerides, total cholesterol and high-density lipoproteincholesterol). The study was carried out in the IVth Clinic of University of
Medicine and Pharmacy “Victor Babes” Timisoara from January 2008 to
January 2009. Results: The total number of patients who met the criteria
for metabolic syndrome was 36 (35%), 38% of them were males and
62% females. Prevalence of the syndrome was 29.7% among 40- to
55-year-olds and 45.6% in those above the age of 55 years. Among the
102 hypertensive patients, type II diabetes mellitus was found in 55.4%,
impaired fasting glucose in 9%, high plasma triglycerides in 42.5% andlow high-density lipoprotein cholesterol in 63.5%. Obesity measured
as BMI = 30 kg/m2 was noted in 48% patients and increased waist
circumference in 62% patients. Conclusion: These findings demonstratethat the prevalence of metabolic syndrome is high among hypertensive
patients and that it is generally poorly diagnosed and treated.
Keywords: metabolic syndrome, hypertension, obesity, BMI

21.DIABETES MELLITUS - DIGESTIVEIMPLICATIONSANDRADA BRATU1, MONICA BRATU1, ADRIANA BUCATĂ1,
ALEXANDRU CROITORU2, SORIN ARAMĂ28

2nd year students,, UMF „Carol Davila”, The Faculty of Dentistry,
Bucharest
2
The Patophisyology and Imunology Department, UMF „Carol Davila”,
The Faculty of Dentistry, Bucharest
1

Introduction. Diabetes is a serious metabolic disease, characterized bypermanent hyperglycemia and severe intermediate metabolic derangements.
Many patients with diabetes present digestive implications. The purposeof our research was to compare the correspondence between digestive
symptoms and the endoscopic exam and we discovered digestive
implications of neuropathy on a group of diabetics compared to nondiabetics. Materials and method. We studied the files of 45 patients (25diabetics and 20 non-diabetics) hospitalised in the Internal Diseases
and Rheumatology „Dr. Ion Cantacuzino” Clinical Hospital, Bucharest,
between january and april 2009. They also underwent an endoscopic
exam. We distributed the patients by age groups, sex ratio, digestive
symptoms, endoscopic lesions, and we made clinical-endoscopic
corellations. The data from this research is represented in diagrams
where we can observe the prevalence of diabetics by age group andby sex. We concluded that in diabetic patients, there are lesions ofesophagitis or ulcer but without them experiencing any pain, therefore
endoscopic exams must be performed on diabetic patients even in theabsence of any symptoms. The non-diabetic patients with endoscopic
injuries presented pyrosis or left hypochondrium and epigastrum pain,
so we revealed a correlation between clinic signs and endoscopy.

22.EXPRESSION OF CATHEPSIN-D IN BREASTLESIONIULIA BRUJAN1, CL. MĂRGĂRITESCU2, CRISTIANASIMIONESCU2, D. PIRICI3, CAMELIA FOARFĂ2, A. STEPAN4,
MARIA VRABETE1
Department of Physiopathology, University of Medicine and Pharmacy,
Craiova2
Department of Pathology, University of Medicine and Pharmacy,
Craiova3
Department of Histology, University of Medicine and Pharmacy,
Craiova
4
Laboratory of Pathology, No. 1 Emergency County Hospital, Craiova

1

Cathepsin-D (CathD) is an aspartyl lysosomal protease expressedin all tissues that might play a role in antigen processing, cellproliferationand tissue renewal, and activation of different pro hormones. The aim ofour study was to compare the expression of CathD (we investigated thecathepsin-D expression in these breast lesions using immunohistochemistry)in most common breast tumors and tumor-like breast lesions. The study
includes 21 patients with histologically verified breast lesions Cathepsin-D
staining within each lesion was assessed by estimating the area ofthe objects and the medium pixel intensity per object, as the integrated
optical density (IOD). The immunostaining was more obvious in breast
invasive carcinomas and macrophages. The reaction in tumor tissue
was heterogeneous with little variation of staining intensity in positive
tumor cells. Results suggest that CathD expression was strongest in
malignant than in benign breast disease, the positivity being present
in both epithelial neoplastic and stromal cells.
Keywords: breast, carcinoma, cathepsin-D,
immunohistochemistry.

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences

23.P 53 EXPRESSION IN BREAST LESIONIULIA BRUJAN1, CL. MĂRGĂRITESCU2, CRISTIANASIMIONESCU2, D. PIRICI3, CAMELIA FOARFĂ2, A. STEPAN4,
MARIA VRABETE1
Department of Physiopathology, University of Medicine and Pharmacy,
Craiova2
Department of Pathology, University of Medicine and Pharmacy,
Craiova3
Department of Histology, University of Medicine and Pharmacy,
Craiova
4
Laboratory of Pathology, No. 1 Emergency County Hospital, Craiova

1

P53 belongs to a multigene family that also includes p63 and p73. A
major role for p53 has been termed “the guardian of the genome” sincea rapid increase of this protein is seen in response to DNA damage(genetic stress), hypoxia or loss of normal cell contacts (epigenetic
stress). The aim of our study was to compare the expression of P53
in most common breast tumors and tumor-like breast lesions. The
study includes 21 patients with histologically verified breast lesions(adenosis, ductal hyperplasia, fibroadenomas, and different types ofinvasive carcinoma). We investigated the P 53 expression in thesebreast lesions using immunohistochemistry (IH; paraffin-embedded
tissues). P53 was the most expressed as intensity and quality in the
low differentiated, invasive ductal adenocarcinoma. Also we had thehighest quantity and quality scores for the most advanced stages(IIIand IV) of those carcinomas. We used a heterogenous marking typeand evidentiated both positive and negative nucleus for the P53 marker,
from one region to another of the carcinoma. Using the same marking
we obtained similar results in the other carcinoma variatis included inour study but with quantity and quality score inferior to those of theinvasive ductal carcinoma. A weak trace of P53 was observed in somebenign lesions of the mammary gland included in our study such as thesclero-adenosis, mammary fibroadenoma, and the intraductal hiperplasia.
Numerous studies have also demonstrated a positive association between
p53 mutation/accumulation and compromised disease-free survival andoverall survival, but whether p53 status provides independent prognostic
information in addition to classical prognostic variables has been acontroversial issue, and opposite results exist as well
Keywords: breast, carcinoma, P53, immunohistochemistry.

24.BOTH NEUROGENESIS AND NEURONAL
DEGENERATION CAUSES GENERALIZEDEPILEPSY
ANA-MARIA BUGA1, AUREL POPA-WAGNER2
University of Medicine and Pharmacy, Craiova, Romania
Klinik für Neurologie, Ernst-Moritz-Arndt-University, Greifswald,
Germany

12With continued debate over the functional significance of adultneurogenesis, identifying an in vivo correlate of neurogenesis has
become an important goal. Kindled seizures are widely used to model
epileptogenesis, but the molecular mechanisms underlying the attainmentof kindling status are largely unknown. Previously we showed that
achievement of kindling status in the Sprague-Dawley rat is associatedwith a critical developmental interval of 25 ± 1 days. We hypothesized

Fiziologia ‑ Physiology  2009 Supplementthat cellular differentiation events with this periodicity may underlie thetightly circumscribed window for the optimal induction of convulsive
seizure. By quantitative immunohistochemistry and confocal 3D-image
reconstruction analysis using tissue from this new model, we now reportthat convulsive seizure leads first to a non-specific, transient increasein the number of proliferative cells that are proliferating cell nuclear
antigen-, BrdU- and doublecortin-immunopositive. However, repeatedconvulsive seizures with a periodicity of 25 days led to an enrichmentof newly generated neurons, which were BrdU-positive in the dentate
gyrus and temporal cortex at day 25 post-seizure. At the same time,
there is a massive increase in the number of neurons expressing the
migratory marker, doublecortin, at the boundary between the granule cell
layer and the polymorphic layer in the dorsal hippocampus and at thelateral ventricle-corpus callosum boundary, some of which are positive
for BrdU. By gene profiling and real-time PCR we show that a numberof neurogenesis-related genes are downregulated in the hippocampusof kindled rats. Moreover, drugs known to enhance neurogenesis
like L-NAME increased the seizure susceptibility at 25 days whereas
drugs known to inhibit neurogenesis like temozolomide, reduced theseizure susceptibility at the critical interval. Paradoxically, prolonged
temozolomide treatment increased seizure susceptibility during thecritisal period by increasing the number of abnormal neurons suggestingthat both increased neurogenesis and abnormal neurogenesis may leadto increased seizure susceptibility. This model may be can be used as
an in vivo correlate of neurogenesis to study basic questions related toneurogenesis and neurogenic mechanisms of epilepsy.

25.The Analysis of the Blood Glucose Control
System in the Frequency Domain
Marius Capra, Ana Dragomirescu, Ionela Iancu,
VERONICA SFREDEL
Physiological the plasma glucose oscillations are the result of theinsulin secretion oscillations and external perturbations (meal, exercise,
medication, stress, etc.). For this study, we have selected 18 adult subjects(10 female and 8 male), patients with insulin dependent mellitus diabetes
and 3 healthy humans. 16 patients underwent treatment with rapid andsemi-lent types of insulin and two patients have received insulin by insulin
pump. The blood glucose was recorded to each patient at five minutes
intervals, continuously for three days, using the Real-Time Guardian
Continuous Glucose Monitoring System in unrestrained conditions.
Each patient had a normal life, with usual meals and activities at workand at home. The continuous blood glucose records represent for thisstudy time-series of the blood glucose concentration. The spectral
analysis of the signal difference between the 3 days of the recording
has displayed a similar behaviour for the three categories of the subjectsof the experimental lot. We have considered that the blood glucose
oscillations over the 0.6 – 0.7 mHz correspond to the function of a very
robust structure from the blood glucose control system that work almost
identically everyday. We have supposed that this structure represent
the mass of cells up taking glucose from the blood stream for the basal
metabolism and the minimum daily activities. The oscillations in the rangeof 0.1 – 0.7 mHz could be the expression of the external perturbations.
The physiological interpretation of this phenomenon is very difficult in
the absence of the experimental studies in this field of interest.
Key words: diabetes mellitus, continuous glucose monitoring,
spectral analysis.

9Official Journal of the Romanian Society of Physiological Sciences

26.SIMVASTATIN REDUCES FIBRINOGEN LEVELSIN PATIENTS WITH HYPERCHOLESTEROLEMIAAND SYSTEMIC HYPERTENSIONCAMELIA CAPUCI¹, MITRAN MARIA CRISTINA¹, SCOREIDARIA IULIA ¹, DRAGOS ALEXANDRU¹, GEORGE BOERU²,MARIA IANCAU¹¹University of Medicine and Pharmacy of Craiova²Department of Cardiology, Emergency Hospital of Slatina
Background:The 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase inhibitors (statins) have multiple actions above andbeyond that of cholesterol lowering. These pleiotropic actions include
direct effects on vascular tissue, kidney, bone, and glucose metabolism.
Statins have a variable response on fibrinogen. Plasma fibrinogen isstrongly asociated with cardiovascular morbidity and mortality. Fibrinogenlevels and plasma viscosity may be used to stratify cardiovascularrisk in hypercholesterolemic patients. Several studies examining
the influence of statins on fibrinogen levels in hypercholesterolemicpatients have shown mixed results. Objectives: The aim of the study
was to compare the effect of treatment with two different doses ofsimvastatin on plasma fibrinogen in patients with hypercholesterolemia
and systemic hypertension. Methodes: Sixty patients enrolled into study
were randomly divided into two groups, treed with simvastatin 20 mg
or 40 mg. Plasma lipid profile and fibrinogen levels were measured
before and after 4 and 12 weeks of the therapy. Results: After 4 weeks
both doses tended to decrease fibrinogen levels, while after 12 weeks
fibrinogen level was significantly decreased. Conclusions: simvastatin
reduces plasma fibrinogen in patients with hypercholesterolemia andsystemic hypertension.

27.NEW STRATEGIES ON HYPERCHOLESTEROLEMIATREATMENTCAMELIA CAPUCI, DRAGOS ALEXANDRU, MARIA IANCAU
University of Medicine and Pharmacy of Craiova
Statins are effective drugs for lowering low-density lipoproteincholesterol, and their use has been associated with a significant
decrease in cardiovascular morbidity and mortality.The benefitsof these medications have been demonstrated in clinical trials forsecondary prevention -controlling risk factors and preventing death inpatients who already have heart disease. Statins may be taken alone
or taken with other cholesterol medicines such as fibric acid derivatives,
bile acid sequestrants, or nicotinic acid. The efficacy of fibrate-statin
combinations was documented in clinical studies showing statisticallysignificant reductions in LDL and triglycerides and increases in HDL.
Several studies showed that the combination of niacin and a statin ishighly effective for treatment of dyslipidemia and is superior to either
drug alone. Ezetimibe can be administered in combination with a statinin patients who are unable to tolerate large dosages of statins or require
further reductions in LDL despite maximum statin dosage.

10

28.CLINICAL ASPECTS OF PERIODONTAL DISEASEASSOCIATED WITH DIABETES MELLITUS
CLAUDIU CĂLIN, ALEXANDRU CROITORU, ŞTEFAN SORINARAMĂ
Carol Davila University of Medicine, Dental Medicine Faculty,
Bucharest
Through Periodontal Disease we define a complex of inflammatory
affections of the marginal parodontium determined by the micro-organisms
in the dental plaque with the contribution of local and systemic factors,
which induce to the organism a particular susceptibility. There area series of oral modifications associated to diabetes mellitus, fromwhich periodontal disease is the most important. Besides gingivitis
and periodontitis appear: dental caries, salivary glands dysfunction andoral infections such as candidosys. Periodontal diseases, as much as
diabetes mellitus, especially type 2 have major inflammatory components.
Systemic bacteria infections produce major systemic inflammations whichraise insulin resistance and therefore glycemia cannot be stabilized. We
have performed a retrospective study over a group of 16 patients withinsulin independent diabetes mellitus hospitalized in the Clinic of the
National Institute of Diabetes and Nutritional Diseases “N. Paulescu”,
in Bucharest, in March – April 2009. The patients have ages between
30 and 60 years old, almost half of them having the glycemia stabilized.
At the objective examination, we have identified more severe odontoperiodontal lesions at the other 9 diabetics that were not glycemia
stabilized. In our study we’ve seen that the diabetic patients with poor
glycemia control through diet have a pronounced degree of teeth mobility
with massive gum retractions and exteriorized root portion, caries at
the neck of remaining teeth, abundant bacterial plaque, massive tartar
deposits, dry lips and xerostomie. We saw that DM determines a modified
immune cells phenotype which increases the production of cytokinesand predispose to a chronic inflammatory process.
Key words: periodontal disease, diabetes mellitus, bacterial plaque,cell phenotype.

29.THE EFFECTS OF PLIOMETRIC TRAINING OVER
THE ATHLETIC PERFORMANCES OF A PUBERTY
AGE SPORTSMEN LOTMIRELA LUCIA CĂLINA1,2, DENISA ENESCU BIERU1, LIGIA
RUSU1,2, ELENA TAINA AVRAMESCU1,2, ANAMARIA KESE1
Faculty of Physical Education and Sport Craiova, University of Craiova,
Romania2
Sport Polyclinic Craiova, Romania

1

Introduction: The pliometric training allows the sprints speed andvertical jump improvement, with important and multiple implications in the
medical recovery. Objective: The purpose of our work was to emphasize
the effects of pliometric training over the sprints speed and vertical jumpat a puberty age football player’s lot, without increasing the injury risk.
Material and method: The study was made on 12 sportsmen, males, witha medium age of 12,5 years, clinically healthy, without joint or muscularpathologies, divided aleatory in 2 groups: group A (6 sportsmen), which
benefit of a specific football training and group B (6 sportsmen), which
additionally benefit of a pliometric training (sprints of 30 meters andFiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
different vertical jumping types). The training period lasted 10 weeks,
under medical surveillance; its efficiency was appreciated before andafter the training programme with the help of the following parameters:
squat jump (SJ) test and speed measurements at different intermediarydistances. Results: At the end of the training period, the group B sportsmen
improved their performances at all levels (the vertical jump increases
with 34% and sprint times decrease with 2%), due to muscular fibers
volume growing, a better neuronal command and intra, inter muscular
coordination, in comparison with the group A, whose results remained
unmodified. Conclusions: The moderate pliometric training determined
the sprints speed and vertical jump (athletic performances) improvement,
without increasing the injury risk at young sportsmen.
Key words: pliometric training, puberty age, sportsmen, athletic
performances.

30.EFFECTS OF ACUTE AND CHRONIC STRESSON CUTANEOUS NEUROGENIC INFLAMMATORYRESPONSE IN RATSCONSTANTIN CĂRUNTU1,2, BOGDAN PAVEL1, LUISA GEORGIANA
BÂCĂ1, ANA LUCA3, LEON ZĂGREAN1
Center for Excellence in Neuroscience, Carol Davila University of
Medicine and Pharmacy, Bucharest, Romania
2
Department of Dermatology, National Institute of Diabetes, Nutrition
and Metabolic Diseases “N. Paulescu”, Bucharest, Romania
3
Faculty of Dentistry, Carol Davila University of Medicine and Pharmacy,
Bucharest, Romania

1

Introduction: The purpose of this study is to examine how acute andchronic stress influences cutaneous neurogenic inflammation inducedby capsaicin. Materials and methods: A number of 15 adult male Wistar
rats (250–350 g) were randomly divided into three groups: control (n 5),acute stress (n 5) and chronic stress (n 5). Stress was administered byplacing each rat in normal ventilated Plexiglas restrainers, a procedure
meant to trigger psychological stress, through the animals perceptionof confinement. Control rats were left undisturbed in their home cages,
animals from acute stress group were exposed to stress for 2 hours andrats from the chronic stress group were stressed 2 hours per day for
21 days. Rats were anesthetized with chloral hydrate. Then, capsaicin
solution 1% was injected subcutaneously in the rat’s paw. Local bloodflow was monitored with a LASER Doppler flowmeter, 10 minutes before
and 30 minutes after capsaicin administration. Results: The cutaneous
blood flow rate before capsaicin administration was similar between
control, acute stress and chronic stress group and was considered asbaseline. In the first 30 minutes after capsaicin injection, blood flow rateincreased in average with 243% in the control group, with 530% in theacute stress group and with 189% in chronic stress group. Conclusions:
The present study shows that acute stress enhances, while chronic
stress decreases capsaicin-induced cutaneous neurogenic inflammatory
response. These findings suggest that stress plays a complex role in theonset and exacerbation of several inflammatory skin disease.
Key words: stress, neurogenic inflammation, capsaicin.

Fiziologia ‑ Physiology  2009 Supplement

31.FROM PRIONS TO PRIONIC DISEASESGEORGIANA CĂTĂLUI
„Titu Maiorescu University“, Bucharest, Medicine Student
The prion hypothesis ignored the so-called “central dogma of
biology” by accepting that protein particles are able to directly produce
diseases without the apparent participation of any nucleic acid,whether this is DNA or RNA. A prion is a protein that is capable of self
replication, thereby altering a cell’s metabolism. Prions propagate in a
retrograde direction, from the axons (the long fibers that emerge fromthe neuron and transmit messages to other cells) to the soma (the mostvoluminous part of the neuron, where the genetic material is found).
Prions are misfolded proteins, causing a variety of prionic diseases
which attack the brain. Prions are the infectious agents responsible
for a group of fatal neurodegenerative disorders. Prionic diseases
are a family of rare progressive neurodegenerative disorders. Thesediseases have enormous variability in their incubation periods, ranging
from a few months to forty years. The prionic diseases begin slowly
and get worse over time, attacking the nervous system. We discuss
the clinical picture, epidemiology, and historical background of prionicdiseases. Prions are responsible for a number of previously known
but little-understood diseases generally classified under transmissible
spongiform encephalopathy diseases (TSEs), including scrapie (a diseaseof sheep), kuru (found in members of the formerly cannibalistic Forétribe in Papua New Guinea), Creutzfeldt-Jakob disease (CJD), Chronic
Wasting Disease, Fatal Familial Insomnia (FFI), Gerstmann-SträusslerScheinker syndrome (GSS), and bovine spongiform encephalopathy(BSE or mad cow disease). These diseases affect the structure of brain
tissue and all are fatal and untreatable.
Key words: prions, infectious agents, prionic diseases.

32.THE DIAGNOSTIC VALUE OF EEG IN
DIFFERENTIATING BETWEEN SUBCORTICALVASCULAR DEMENTIA AND ALZHEIMER’SDISEASE
AURA CIOBANU1, M. COTEANU2, SIMONA GUSTI3, ALICEGUSTI4
Neurology Clinic, Clinical Neuropsychiatry Hospital, Craiova,
Romania2
Departament of Neurology, County Hospital, Slatina, Romania
3
Departament of Physiology, University of Medicine and Pharmacy,
Craiova, Romania
4
Departament of Physiology, Faculty of Physical Education and Sport,
University, Craiova, Romania

1

Objective: The study was undertaken to investigate whether visual
EEG may be used in differential diagnosis between Alzheimer’s disease(AD) and subcortical vascular dementia (SVD). Methods: We analyzed
EEG in 21 patients with AD (according to NINCDS-ADRDA criteria)
and 14 patients with probable SVD (according to NINCDS-ADRDA and
Erkinjuntii’s criteria). The patients were divided into two subgroups withmild and moderate dementia according to the scores of Mini Mental
State Examination (MMSE). The EEG findings were classified using
an eight degree scale according the background activity, presence and11Official Journal of the Romanian Society of Physiological Sciences
amount of theta and delta waves. Results: The visual EEG as alpha/thetaand delta ratio differed subgroups with moderate dementia but did not
differ AD and SVD with mild dementia. Conclusion: Visual EEG could
only differ AD and SVD groups with moderate dementia, and probably
QEEG could be useful in differential diagnosis between AD and SVD
groups with mild dementia in early state of disease.

33.A STUDY OF BLOOD PARAMETERS IN PATIENTSWITH PAROXYSTICAL ATRIAL FIBRILLATION
CĂTĂLINA CIORNEI, RALUCA PAPACOCEA, ANCA BĂDĂRĂU,MARIANA ARTINO, ANDREI GEORGESCU, DUMITRU FERECHIDE,MIOARA ZAMFIR, SILVIA NICA, FLORINA CIORNEI
Carol Davila University of Medicine and Pharmacy, Bucharest
Paroxystical atrial fibrillation (AFi) is an arrhytmia with sudden
onset, that produces important and characteristic symptoms, so thatthe patients immediately go and see the doctor. Unlike this situation,
the patients with chronic atrial fibrillation have almost no symptoms,so that they go and see the doctor at more than 48 hrs after the onsetof the arrhytmia. We studied some blood parameters in patients withparoxystical atrial fibrillation , in order to predict the further evolutionof the sudden onset arrhythmia. Material and methods: we studied 11
patients presented in the Emergency Department of the Emergency
University Hospital in Bucharest, with paroxystical atrial fibrillation. We
studied the parameters at the admission in the ER, at 4 and 6 hrs later.
The patients received antiarrhytmic drugs (propafenone or amiodarone),some of them converted into sinus rhythm (SR) and some of them did
not. We made two groups of patients, those that converted in SR andthose that remained in AFi. We also compared the parameters in thepatients with the first episode of AFi and those with recurrent paroxysticalatrial fibrillation. We studied the serum level of NT-proBNP, myoglobine,
CK-MB, CRP, TGO, TGP.
Key words: paroxystical atrial fibrillation, recurrent, NT-proBNP.

34.THE VARIATIONS OF SOME BIOCHEMICALPARAMETERS AND OF THE CLOTTING SYSTEM IN
PATIENTS WITH ACUTE POISONING WITH CO
CATALINA CIORNEI1, ANCA BADARAU1, MARIANA ARTINO1,
A. GEORGESCU2, ANTOANETA PUNGA1, SILVIA NICA2, D.
FERECHIDE1, M. LUPUSORU1, MIOARA ZAMFIR2, RALUCAPAPACOCEA1, S. CONSTANTIN2
Department of Physiology I, University of Medicine and Pharmacy“Carol Davila”, Bucharest
2
Emergency Department, Emergency University Hospital, Bucharest
1

The poisonings with CO are accidental, more frequently as domestic
emergencies. As a result, this kind of poisoning is important because
of:-the difficulties in establishing the right diagnose in the cases the CO
acts along with other poisoning gases;-the high frequency and the type of
poisonings.In our study we followed up the AST and ALT as biochemicalparameters, and the number of platelets, APTT and INR, as the clotting
system parameters.The study was made on 41 patients, males andfemales, grouped in:-group 1, made of 15 patients, aged 40-83 yrs;group 2, made of 26 patients, aged 17-40 yrs.The measurements were12

made at the admission and 6 hrs later.The results showed:-insignificant
changes of AST and ALT in both groups;-decrease (slightly significant)of the number of the platelets after 6 hrs in group 1;-increase (slightly
significant) of the INR after 6 hrs in group1;-different variations of APTT
after 6 hrs in both groups.

35.ERRORS IN LOCATION EPILEPTIC FOCUS
DAN ŞTEFAN CIOROIANU1, ADRIAN NEŞTIANU2, FLORINROMANESCU3, MARIUS CRISTIAN NEAMŢU1, TUDOR-ADRIANBĂLŞEANU3, SMARANDA IOANA MITRAN3¹University of Medicine and Pharmacy Craiova, Faculty of Midwives
and Medical Assistance, Department of Normal and Pathologic
Physiology
2
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Pathologic Physiology
3
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Normal Physiology
In mapping the brain, under the form of maps, energy is converted
into an area that takes the appearance of a true geographic relief.
Depending on way of monopolar reference collection, a comitial focus
can be shown in the side opposite the real one.There were used adigital electroencephalograf and monopolar collections (standard Cz,
and average AC - Laplace), EEG recordings were made under standard
conditions. Each patient was subject to amplitude, frequency, energy,
delay mapping, as well as Burchi and FFT mapping.
By direct reference (with referential electrode Cz which is close to
the lesion focus) EEG route is ample, but the mapping, does not indicate
the genuine, real focus in the left side, anterior to posterior, due to thecontamination of the referential electrode A2 (right auricular) with large
comitial potentials arisen from the temporal electrodes near the rightear. Paradoxically, the mapping indicates a certain higher activity in left
side where the right focus is less propagating “in the mirror”. If averagereference is used, the routes are less extensive and the real focus on
the right appears prior to posterior -  opposed to the previous image
since average virtual reference was not contaminated. Using Laplacereference  comitial focus (inter-critical) appears more circumscribed in
two separate points anterior and posterior in the right, and the routes
are less extensive. For an adequate sample of cortical electrical field
is preferably used polynomial interpolation between areas measured
rather than linear interpolation, in order to reconstruct the real aspectof the map relief.
Key words: mapping, EEG

36.THE ENDOCANNABINOID SYSTEM: IT’S KEY
ROLE IN THE APPEARANCE OF THE CARDIOMETABOLIC RISK FACTORSPETRIŞOR CISMAŞ-PRUTEANU, MAGDALENA CISMAŞPRUTEANU, CEZAR GEORGE BADALE, ANDREA CISMAŞPRUTEANU
University of Oradea, Faculty of Medicine and Pharmacy
Endocannabinoids (ECs) are endogenous lipid substances which
elicit biological effects similar to those of marijuana via activation of

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
G protein-coupled cannabinoid receptors (CB1 and CB2, as well as
additional, as yet unidentified receptors). The two best characterized ECs
are arachidonoyl ethanolamide (anandamide) and 2-arachidonoylglycerol( 2-AG). Both CB1 and CB2 receptors are expressed in the myocardium
and the vasculature. ECs and their synthetic analogs exert potent
hypotension via CB1 receptor-mediated sympathoinhibitory, negative
inotropic, and vasodilatory effects. Activation of endothelial CB2 receptors
by endogenous or exogenous ligands limits endothelial inflammatory
response, chemotaxis and adhesion of inflammatory cells to the activatedendothelium, and consequent release of various proinflammatory
mediators (key processes involved in the initation and progressionof reperfusion injury and atherosclerosis). CB2 receptor activation
on immune cells mediates additional anti-inflammatory properties.
Selective CB2 agonists may be useful in the treatment of various formsof reperfusion injury. Activation of the EC system has been implicatedin hypotension and/or decreased myocardial contractility associatedwith hemorrhagic, endotoxic, septic, cardiogenic, shock, advanced livercirrhosis, heart failure secondary to doxorubicin treatment, and as acompensatory mechanism in various forms of hypertension. Activationof the EC system contributes to the cardiovascular risk associated withobesity/metabolic syndrome and diabetes (abdominal obesity, plasma
lipid alternations, insulin and leptin resistance).
Key words: endocannabinoid system, CB1 and CB2 receptors,
cardio-metabolic risk factors.

37.PREDICTIVE MARKERS FOR EARLY LIVERFIBROSIS DEVELOPMENT IN TOXIC HEPATITISEVOLUTION
S.CLICHICI1, C.CATOI², T. MOCAN1, A. FILIP1, C LOGIN1, A. NAGY²,
D.DAICOVICIU1, N. DECEA1, S.SUCIU1, A. MUREŞAN1
Department of Physiology, “Iuliu Hatieganu” Univ of Medicine andPharmacy Cluj-Napoca, Romania²University of Agriculture and Veterinary Medicine, Morphopathology
Department, Cluj-Napoca, Romania
1

Introduction: Carbon tetrachloride (CCL4) induced hepatitis isan effective experimental model for the study of the liver dysfunction,diffuse fibrosis and cirrhosis. We used the CCL4 model to correlateoxidative stress markers with the presence of liver fibrosis. Our study
aimed to identify predictive, non-invasive parameters of early fibrosis
development in toxic hepatitis evolution. Material and Method: The
20 animals (male Wistar rats, weight 200±10 gr) were randomly andequally divided into two experimental groups. CCL4 was intragastricallyadministered twice a week to the test group, (1.2 ml/kg, CCL4 25% insunflower oil). Control group received sunflower oil following the same
standard administration protocol. After 4 weeks of treatment, plasma
levels of malondialdehyde(MDA), carbonylate proteins(CP), hydrogen
donor ability (HD), sulfhydryl groups(SH), gluthation(G) and nitricoxide(NO) were measured. Histological examination of the liver was also
performed. Statistical analyses included non-parametric Mann-Whitey
U Test as well as receiver operating characteristic analysis with areas
under curve (AUROC) determinations. Results: Centrolobular fibrosis
with vacuolar dystrophy and hepatocitar necrosis (individual cells)
was histologically demonstrated in the CCl4 treated group. Significant
differences between groups were obtained for SH(p0.045), DH(p0.011),
PC(0.024). GH levels were marginally significant (p0.070). Predictive
values of the four markers were validated by AUROC values. The highest

Fiziologia ‑ Physiology  2009 Supplement

predictive marker was DH(0.871, p<0.0001), followed by PC(0.829,
p0.003), SH(0.793, p0.014) and G(0.764, p0.034). Conclusions: Ourdata suggest that oxidative stress markers may become useful predictive
markers of early fibrosis development in toxic hepatitis.
Keywords: oxidative stress, predictive markers, toxic hepatitis,
early fibrosis

38.ANGIOTENSIN INVOLVEMENT ON ADENOSINEINDUCED BRONCHIAL HYPERREACTIVITY
COJOCARU ELENA1, PETRESCU BOGDAN CRISTIAN2,
DUMITRIU IRINA LUCIANA1, COSTULEANU MARCEL2, GURZUBOGDAN1, SLATINEANU SIMONA MIHAELA1
Department of Physiology, School of Medicine
Department of General Pathology, School of Dentistry “Gr T Popa”
University of Medicine and Pharmacy Iasi, Romania

1

2

It is known that adenosine is an endogenously purine nucleosidewhich plays a significant role in regulation of airways tone and reactivity
by multiple and incompletely known mechanisms, including the releaseof endogenously active peptides from mast cells. Published datasuggest that releasing of enzymes from activated mast cells could
activate the intrapulmonary renin angiotensin system (RAS). In thisstudy, we investigated the involvement of angiotensin II (Ang II) in
adenosine-induced bronchoconstriction in an experimental model ofallergic asthma. On bronchial rings from ovalbumine (OVA) sensitized
rats, after in vitro challenge, adenosine induced small contractile effects
which became significantly after indometacine pre-treatment. On the other
hand adenosine pre-treatment amplified bronchoconstriction inducedby the allergen (OVA) challenge and reduced bronchial relaxation ofacetylcholine pre-contracted bronchial rings by cumulative doses ofterbutaline. All these effects are significantly lower on rats treated withlosartan (a blocker of Ang II type 1 specific receptors, AT1) in the last
two weeks of sensitization protocol (50 mg/kg/day). Our data confirmed
the participation of RAS activation to adenosine induced bronchial
hyperreactivity in pathologically states as antigen sensitization andchallenge.
Key words: adenosine, angiotensin, asthma, rat

39.A FRACTAL ANALYSIS OF CORNEAL
VASCULARISATIONLAURENTIU COMAN1, OANA COMAN2, ANCA BADARAU1
Department of Physiology 1, University of Medicine and Pharmacy“Carol Davila”, Bucharest
2
Department of Pharmacology and Pharmacotherapy, University of
Medicine and Pharmacy “Carol Davila”, Bucharest
1

Various pathological conditions may cause vascularisation of thenormally avascular and transparent cornea. The corneal pathologic
vascular tree exhibits fractal characteristics. The purpose of this study
was to investigate the fractal geometry of corneal vascularisation andrelate it to different growth models which may have implications in theunderstanding of fundamental process involved in vasculogenesis. Digital
photographs of 18 cases of severe superficial corneal vascularisation
were obtained from accidentally alkali burned eyes long after the acute13Official Journal of the Romanian Society of Physiological Sciences
inflammatory reaction was over. The fractal dimensions of these vessels
patterns was calculated by means of the density correlation function
method (D1,893±0.004), n. These results suggest the importance ofthe surface characteristics of the invading cells as well as that of theextracellular matrix and the related interaction between them.
Key words: corneal vascularisation, alkali burns, fractal analysis.

40.ASPECTS OF VASOMOTOR REACTIVITY AND
CEREBRAL DOPPLER VELOCIMETRY TO A LOT
OF ALZHEIMER\’S DISEASE PATIENTS
M. COTEANU1, AURA CIOBANU2, SIMONA GUSTI3, ALICEGUSTI4
Departament of Neurology, County Hospital, Slatina, Romania
Neurology Clinic, Clinical Neuropsychiatry Hospital, Craiova,
Romania3
Departament of Physiology, University of Medicine and Pharmacy,
Craiova, Romania
4
Departament of Physiology, Faculty of Physical Education and Sport,
University, Craiova, Romania

1

2

Introduction: A close relationship between Alzheimer’s disease andthe cerebral vasculature has long been recognised.
Recent studies have reported that the severity of atheromatous
deposits in the circle of Willis is significantly greater in subjects withneuropathologically diagnosed Alzheimer’s disease (AD), comparative
to non-demented people (ND).
Objective: The aim of the study was to examine the vascular integrity
and assess the vasomotor reactivity of Alzheimer’s disease patients incondition of hypoxia caused by breath holding. Methods: We studied 12
patients with diagnosed Alzheimer’s disease, using transcranial Doppler.
Vasomotor reactivity was calculated used breath holding index .
Statistically analysing dates of Doppler parameters: mean velocity
flow of the internal carotid artery siphon, the middle cerebral artery andthe posterior cerebral artery right and left in basal condition and mean
velocity flow of the middle cerebral artery in apneea, we calculated
breath holding index and compared to the parameters of a group of 14
clinically healthy persons. The patients were divided into two subgroups
and were staged according to Mini Mental State Examination (MMSE).
Results: Mean flow velocities were generally lower in patients with AD but
the differences did not reach the significance level. The internal carotid
artery siphon and the posterior cerebral artery were the two vesselsthat were strongly associated with AD diagnosis. Mean flow velocities
were systematically lower in the group whose MMSE scores were <20than the ones whose MMSE scores were >20. The AD patients had
significantly lower Breath Holding Index (p<0.005) than the ND group,and no significant differences between two subgroups. Conclusion:
Our datas make consider that there is a posible relationship between
the the vasomotor reactivity and Alzheimer’s disease.

41.A study regarding the possibility ofusING PREDICTive control for blood
glucose system
VIORELA CristEa, DANIELA DINCă, VLAD FLORIN IOVĂNESCU,ELENA PREDUŢ, IONELA IANCU

The mellitus diabetes is a disease with serious social implications
through the large number of people affected, complications and high costs
that it involves. The realisation of the artificial pancreas is presently afundamental high priority research with a strong interdisciplinary character.
We propose a procedure to design a linear output-feedback controller
for blood glucose system. The controller stabilizes the system in thepresence of delays, sampling, etc. The closed-loop system with delays,
sampling, can be modeled as delay differential equations. Numericalsimulations show that the proposed design method is significantly better
than the existing ones. The direct insulin control system for insulin pump
adapts the necessary insulin quantity in the case of food intake, physicaleffort, during sleep or at patients found in a critical state and assisted
in the intensive care section. In these conditions, the determining of
the insulin dose requires a predictive control system in regard to the
disturbing situation. The main advantage of the predictive control consists
in a proper insulin dosage taking into account delays generated by theabsorption and processing of the rapid acting insulin medication.
Key words: blood glucose control, predictive control, mathematicalmodel, artificial pancreas.

42.THE IMPACT OF EMOTIONAL STRESS OF
PUPILS
CRIVOI A., BACALOV IU., GHERMAN I., CASCO D., PRODAN
M., VEGHE EVA-MARIA., MATEI V., COJOCARI L., BÎLICI T.
State University, R. Moldova
Childhood and adolescence are the pillars of formation andimmunization of physic and psyhic healt of human body. Many of psycho
somatic pathologies observed at adults presents the marks remained
after the action of unfouvorable factors and stress stares durin prenatal,childhood and adolescence development. Modern environmeut forces
through the increase of informational volume, intellectual supradoses,
hypokinesis, food deficiency, conflict situations creating predispositions
to homeostatic modifications, great energy loss, vegetative disfunctions.
Physiologic science include numerous investigations of stressogene
difficulties, but still remain unsearced the amaunt of emotional stressand its affects on body development. Emotional stress determines
neuronal, cardiovascular, endocrine, digestive, psihic pathologies, which
are in boosting dynamics, caused by psycho emotional traumas andemotive stress stares. The aim of the research was the study of the
dynamics of psyhologic, cardiovascular, neuronal, hormonal, metabolicchanges under the influence of educational stress at teenagers, and thepossibilitres to avoid advanced stares with pathological concequencesby psychoemotional adaption being correct and equilibrate. The
results showed that resistance aud level of adaptation of teenagers
manifested individual pecularition with over dominance of vagotony andsimpatotony, of the level of anotomophysiologic maturity, level of stableauxienty persistence, ensuring forming of tough personality, adapted
to the variance of body-environment interactions. Teenagers whith
good pshysic education possess the capacities of quick rehabilitationof cardiac contractions and artherial tension, so dynamism efficiency
puts its finger-print on the adaptive tendency of the organism to theaction of stress factors.
Key words: emotional stress, impact, metabolic changes,
adaption

University of Medicine and Pharmacy of Craiova, Faculty of Medicine

14

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences

43.PHYTOTHERAPY OF DIABETESCRIVOI A., BACALOV IU., GHERMAN I., CASCO D., PRODAN
M., VEGHE EVA-MARIA., MATEI V., COJOCARI L.,CROITORIC., BÎLICI T.
State University, R. Moldova
Medicinal plants are widely used in medical practice nowadays in
RM are used over 150 species of medicinal plants as hypoglucemiats at
initial stages of Diabetes. Most of them are well known in clinic medicine,others are still in research. It is considered that they contain a complex
of vitamins and microelements in am optimal combination for organism.
Plants with similar therapeutic effects are named adjuvants. They incrase
the efficiency of the terapy, through the combination of their activity.
The research results showed that the extracts from medicinal plants
possess a positive influence on haematologic indices,biostimulative
proprietes on functional activity of pancreas, thyroid and gonads by
their tendency to normalize hormone content in blood,that represents
an important role in preventing diabetes complicacy vegetal complex
from black berry : floss, roseberry, increased adaptive and stimulator
potential, which was revealed by clinic, haematologic and hormonal
manifestations. This results allov to occlaim that first stages of the disease
there were observed the tendency of normalization of thyroidian status
and significant increase of sexual hormones in blood. This complex ischaracterized by its low toxicity, back of cumulative proprieties and,as usuad, the lach of antagonistic effects, that is very important in thetherapy of diseases with chronic ethiology.
Key words: diabet, medicinal plants, thyroid, medicinal plants

44.THE ANTIOXIDANT EFFECT OF VITAMIN C ON
THE DENTAL CAVITIESCAMELIA DALAI1, ADRIANA PIRTE1, CIPRIAN DALAI1 MARIANA
MURESAN1, OTILIA MICLE1, LIANA MICLE1, RALUCA IURCOV1,
ALINA VENTER1, MIRCEA DOROFTEIU2
1

Medicine and Pharmacy Faculty Oradea, Romania
University of Medicine and Pharmacy , Cluj Napoca, Romania

2

Introduction. Reaserches on the saliva have shown its importancein the diagnoses of varios disease in medical practice. To the propertiesof saliva such as cleansing solution,a lubrificant,an ions reservoire andbuffer it can be also added a first line of defence against ROS mediatedoxidative stress.Vitamin C is an important water-soluble vitamin forhumans and a powerful reducing agent. It also protects plasma lipids
against lipid peroxidation. The aim of our study was to demonstrate
the evolution of the salivary oxidative stress in persons with dental
caries and if the administration of vitamin C improves the antioxidant
capacity and if there are significant modifications of transaminases,
phosphatase, LDH or C-reactiv protein (CRP) in saliva. Material andmethod. The studied persons were divided into 2 groups (each groupconsisting of 16 persons), one group with a single tooth with a carious
process non- smokers, and the otherone with multiple caries. The firstgroup took 200 mg vitamin C 3 time a day, for 3 weeks. For proving thesalivary and serum oxidative stress we established the level of MDA
utilizing the method with tiobarbituric acid and ceruloplasmin with Ravinmethod. GOT and GPT, alkaline and acid phosphatase, LDH and CRP

Fiziologia ‑ Physiology  2009 Supplement

were determined in saliva on analyzer HITACHI 912, Roche Diagnostic,
Switzerland, using reactive Greiner Diagnostic, Germany. The obtained
results were compared to an experimental group of 15 volunteers with nodental lesions or restorations. Resultes. Salivary MDA was significantly
increased in the group with a single tooth with a carious process and in
the group with multiple caries.The same was in blood sample of thosegroups.These researches could be an indirect prove that serum MDA
do not pass in saliva through filtration. Our investigations attested thestrong antioxidant efect of vitamin C even in small doses. Salivary levelof MDA was reduced. The tissues dental destruction and periodontitis
was testified by the increase of salivary transaminses. The presence ofproceses of inflamation was pointed out by the increased concentrations of
LDH and CRP. The bony reshaping caused by the studied dental lesions
was reflected in the variations of alkaline and acid salivary phosphatase.
Conclusions. This findings suggest that there was a high level of MDA
and lower salivary ceruloplasmin level caused by the carious process.
Our study indicates the protective effects of vitamin C in saliva forthe prevention of oral inflamations. The level of GOT, GPT,LDH ,CRP
indirectly indicates the presence of an inflamatory process. Key words:
carious process, stress oxidative, vitamin C.

45.STUDY REGARDING THE OVERALL FLEXIBILITYIN WOMAN WITH MITRAL VALVE PROLAPSDANOIU SUZANA1, DANCIULESCU RUCSANDRA2, SFREDELVERONICA1, CATALINA POIANA2, DANOIU M.3
University of Medicine and Pharmacy Craiova
University of Medicine and Pharmacy Carol Davila Bucharest
3
University of Craiova

1

2

Aims. Connective tissue disorders affecting joints mobility and leaflets
mitral excursion may have common histological and biochemical support
making the woman with mitral valve prolaps (MVP) more flexible than
the general population. The objective of this study was to confirm if theadult woman with MVP presents joints hypermobility comparing withwoman who have not this pathology. Material and methods. We studied
a sample of 98 adult woman, average age 42 yrs, 15 of them was beenpreviously clinical and echocardiographic diagnosed with MVP. The
passive joints movement from 20 kinds of movements was evaluatedperforming the Flexitest and tree simple hyperlaxity tests. Statisticalanalyses were performed by t test for interval data and by the (chi)2
test for ordinal or nominal data. Results. Woman with MVP presented
a higher level of body flexibility comparing with the others without MVP(The Flexindex was significantly higher in the women with MVP - p<.02).
No one of them presented generalized hypermobility.
Keywords: mitral valve prolaps, flexibility, joint hypermobility

46.THE EFFECT OF SOME ANTIHYPERTENSIVEDRUGS ON NITRIC OXIDE SYNTHESIS ANDOXIDATIVE STRESS IN ACUTE EXPERIMENTALINFLAMMATIONDHAOU YOUSRA1, MOHAMMAD SADEK ASHOUR1, TARIGOMER ELAMIN1, PARVU ALINA ELENA2
University of Medicine and Pharmacy ”Iuliu Hatieganu”, Cluj-Napoca

15Official Journal of the Romanian Society of Physiological Sciences
Antihypertensive drugs interact with mediators that are also involved in
the inflammatory responses, such a neuropeptides, adrenergic receptors,
and vascular tone modulators. Therefore, we aimed to study the effectof antihypertensive drugs on NO synthesis and oxidative stress in acute
experimental inflammation. We used 6 groups of Wistar-Bratislawa malerats with turpentine oil-induced inflammation: a positive inflammation
control with no treatment, five groups treated respectivelly with almodipin,
felodipin, telmisartan, valsartan and nifedipin, a group treated with with
L-NAME, and a group treated with trolox. NO synthesis was evaluatedby serum nitrites and nitrates measurement, and oxidative stress wasevaluated by total oxidative status and total antioxidative reactivity
determination. The results showed that al drugs reduced significantlly
nitric oxide synthesis and oxidative stress. The inhibitory effect on NO
synthesis was smaller than that of L-NAME. The antioxidative effectwas not significant. In conclusion, almodipin, felodipin, telmisartan,
valsartan and nifedipin had antiinflammatory effect due to the inhibitory
action on NO synthesis and oxidative stress associated to the acute
experimental inflammation.
Key words:antihypertensive drug, inflammation, nitric oxide,oxidative stress

47.THE ACUTE EPIRUBICIN ADMINISTRATION
INCREASED THE UROTENSIN II CONTRACTILE
EFFECTS ON RAT CORONARY ARTERY
MARIA DINCA, PETRESCU BOGDAN CRISTIAN, VATA LUMINITA
GINA, MIHAI CAMELIA ANA, DUMITRIU IRINA LUCIANA, GURZUBOGDAN, PETRESCU GHEORGHE
Department of Physiology, University of Medicine and Pharmacy “Gr
T Popa” Iasi
In this study we aimed to evaluate the effects of epirubicin
administration on coronary reactivity by measuring isometric tone ofring segments of the isolated rat left anterior descending coronary arteryon wire myograph. The experiments were conducted in two groups ofmale Wistar rats: untreated control rats (NR) and epirubicin-treatedrats (ETR). The left anterior descending (LAD) coronary artery rings
were precontracted with urotensin II (1nM to 1 microM, U_II) and therelaxant responses to cumulative concentration of acetylcholine were
subsequently studied. The experiment were carry out in the absence
and in the presence of N(G)-nitro L-arginine methyl ester (L-NAME)
or aminoguanidine (AG). The results of this study indicate epirubicin
treatment alter the reactivity of rat LAD coronary arteries by increasing
contractile effects of U_II (with more than 33%) and reducing Emax of
ACh-induced vasorelaxation up to 25%. The blunted ACh response of
LAD coronary artery rings from ETR was reduced even more and in
the same manner by both L-NAME and AG. These results suggest that
epirubicin treatment increased U_II contractile effects and decreased theACh – induced relaxation by alter the nitric oxide (NO) synthesis.
Key words: epirubicin, urotensin, left anterior descending coronary
artery16

48.THE ACQUISITION OF COGNITIVE EVOKED
POTENTIALS USING A PSYCHO-VERBAL STIMULATIONINTERFACEBOGDAN DIONISIE
University of Medicine Iasi, Romania
Objective – The psycho-verbal stimulation interface, created byus, represents an IT extension of an EEG / EMG device dedicated forthe human brain Evoked Potentials acquisitions (EPs). Methods - The
psycho-verbal stimulation interface was created as a .NET application
and was written in the C# language with respect to the OOP paradigm. It
uses the TTL logic to command the EPs acquisition module. The signal
is send on the parallel port (LPT1) to the EPs device by a dedicatedhardware interface. Results - The software module was designed as amultithreading application in order to perform more than one operation
once. The stimulus sample can be customized as text, images or bothand each stimulus sequence follows also a fully configurable time schema.
The application can measure the patient’s reaction times to each stimulus
sample, which are centralized finally on a list control. The system cansave both the test sequences and the EEG diagrams in digital formats;the files can be stored and delivered online using the telemes web
application. Conclusions - The Telmes project implemented a secured& scalable telemedical centers network dedicated to the telemonitoring& teleconsulting services, and the psycho-verbal stimulation interface
is one of the instruments devoted to these goals. The psycho-verbal
stimulation interface can be used as a medical research tool for study
the cognitive processes of reading, memory or learning using theendogenous visual event related potentials, as good as a instrumentof training the recovery of sensitive language which can be delivered athome by the neuronlinguist specialist as daily lists programs. The visualevoked potentials and the reaction times collected from the patients canfacilitate a prognostic diagnosis of recovery the language.
Keywords - psycho-verbal stimulation interface for evoked potentials,
language research & recovery, eHealth – telerehabilitation.

49.THE EFFECT OF LEXICAL SIMILARITY IN THE
WRITTEN MESSAGE PRIMING STUDIED BY USINGCOGNITIVE EVOKED POTENTIAL AND REACTIONTIMES TECHNIQUESBOGDAN DIONISIE
University of Medicine Iasi, Romania
Objective – The redundancy among lexical elements in similar word
can create a facilitatory or inhibitory effect in lexical processing and forthis purpose several issues persuated me to evaluate lexical similarity
using ERPs and RT techniques. Methods – I study de N400 component
on Evoked potentials technique (ERPs) which is impervious to decision
based processing while replicating existing behavioral findings. I use lists
of words an pseudowords with low frequency and different degree o lexical
similarity in lexical decision and semantic categorization tasks. Results– Pseudowords from large neighborhoods produced larger negativity inthe region of the N400 than pseudowords from small neighborhoods
and words with large neighborhoods produced large negativities in theregion of the N400 than words with small neighborhoods. Conclusions –Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
The findings from these experiments illustrate how electrophysiological
data can be used to augment more traditional measure such as RT in
studies of language comprehension.
Keywords – lexical similarity of word and pseudowords, event
related potentials ERPs, reaction times RT

50.NITRIC OXIDE AND OXIDATIVE STRESSIN UVEITIS ASSOCIATED WITH RHEUMATIC
INFLAMMATORY DISEASESBIANCA DOBRE1, CRISTINA DOBRE2, CRISTINA STAN1,
ALINA PÂRVU1
1

University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca
Therapy and Diagnostics Center Cluj-Napoca

2

Uveitis is often associated with systemic, rheumatic inflammatory
diseases such as ankylosing spondylitis, sarcoidosis, juvenile idiopathic
arthritis or Behcet’s disease, as an extra-articular manifestation. The
mechanisms are poorly understood. The aim of the study was to evaluate
nitric oxide and oxidative stress involvment in uveitis cases associatedwith inflammatory rheumatic diseases. The study group consist of 30
patients with rheumatic inflammatory diseases. The control group were
30 patients with rheumatic inflammatory diseases without uveitis. In
serum and tears we measured nitric oxide synthesis by Griess test,
and oxidative stress by total oxidative status and total antioxidative
reactivity. According to the results we concluded that in patient with uveitis
associated to rheumatic inflammatory diseases there is a significant
increse of nitric oxide synthesis and oxidative stress, compared to thepatients with rheumatic inflammatory diseases and no uveitis. These
results suggest that reducing nitric oxide synthesis and oxidative stress
may represent an important therapeutical target.
Key words: uveitis, rheumatic disease, nitric oxide, oxidative
stress

51.THE WAY PATIENTS EXPERIENCE FEAR OF
WHITE COAT IN DENTAL OFFICE COMPARED TO
GENERAL PRACTITIONER’S OFFICEDIANA DODONETE1, DIANA CONSTANTIN1, CRISTINA DOBRE1,
MIHAI IOAN1, DANIELA BĂDIŢĂ2, ALEXANDRU CROITORU3
Carol Davila University of Medicine, Dental Medicine Faculty, Bucharest,
1st year students
2
Carol Davila University of Medicine, Dental Medicine Faculty, Bucharest,
Department of Physiology
3
Carol Davila University of Medicine, Dental Medicine Faculty, Bucharest,
Pathophysiology and Immunology Department
1

We have undergone a study on a batch of 40 patients, aged between
35 and 75 years that had come into the doctor’s office during February andMarch 2009. We used a standardized questionnaire and we measured
the patients’ systolic and diastolic blood pressure and the heart rate,in order to compare the way they feel in the dentist’s office and in thegeneral practitioner’s office. We reached to the following conclusions:
Admitted or not by the patients, anxiety is shown to be increased inthose coming for a dental consultation. The white medical coat seems

Fiziologia ‑ Physiology  2009 Supplement

to be a reason for the rising of the determined parameters and for theaugmentation of the anxiety level in almost every patient, compared
to coloured coats. We have also observed that individual parameterssuch as profession, age and gender have different impacts on bloodpressure and heart rate. Profession is not a relevant criterion becauseeven highly educated people enhanced signs of fear. The elders’ bloodpressure fluctuated more than that of the young patients. When comparing
blood pressure variations according to the gender of the patient, weobserved that, although the majority of male patients said that coming
into the doctor’s office doesn’t stress them, the registered values showed
exactly the opposite. Women, on the other hand, admitted that visiting
the doctor scared them which it was actually confirmed by the significant
increased variations of blood pressure and heart rate.

52.PARTIAL METHYLATION OF CPG ISLANDPROMOTER OF IGF-1R IN NON SMALL CELLLUNG CANCER
ANICA DRICU
University of Medicine and Pharmacy of Craiova
Epigenetic modifications have been described to be involved in thepathogenesis of lung cancer. Up till now, three different mechanisms
have been described like epigenetic modifications: RNA-associatedsilencing, DNA methylation and histone modifications. Recently, we
showed that igf-1r- siRNA down-regulated the IGF-1R expression andeffectively induced cell death in NSCLC cells. In this work, we wereinterested to study the IGF-1R methylation status in NSCLC cells andpropose that IGF-1R hypomethylation may provide a basis for potential
use of hypermethylating agents for lung cancers treatment. First, weanalysed the DNA methylation patterns of IGF-1R CpG island in 6
NSCLC cell lines that expressed IGF-1R at the cell surface and foundpartial methylation of igf-1r gene in all cell lines analysed. To determine
whether increasing the igf-1r methylation is associated with IGF-1R
downregulation and thereby inducing cell death in NSCLC cells, we treat
the cells with S-adenosylmethionine (SAM), a hypermethylating agent.
NSCLC cell lines cell lines had different levels of sensitivity to SAM
treatment. Unexpectedly, we found that the SAM treatment did not change
the igf-1r methylation status or the levels of IGF-1R protein expression
in any NSCLC cells analysed, suggesting that SAM-induced cytotoxicity
is independent of igf-1r methylation status in NSCLC cells.

53.THE ACTIVATION OF INTRABRONCHIAL RENIN
ANGIOTENSIN SYSTEM DURING ALLERGIC LUNGDISEASEDUMITRIU IRINA LUCIANA1, PETRESCU BOGDAN CRISTIAN2,COJOCARU ELENA1, CIUREANU IOANA STEFANIA1,COSTULEANU MARCEL2, GURZU BOGDAN1, SLATINEANUSIMONA MIHAELA1
Department of Physiology, School of Medicine
Department of General Pathology, School of Dentistry “Gr T Popa”
University of Medicine and Pharmacy Iasi, Romania

1

2

All components of the renin angiotensin system (RAS) were identified
on bronchial wall and their involvement on pulmonary diseases is well17

Official Journal of the Romanian Society of Physiological Sciences
known. To analyze if antigen sensitization and challenge could modify thesynthesis of angiotensin (Ang) II on bronchial walls and to what extent
locally produced Ang II modulates bronchial reactivity. Bronchial rings
were obtained from normal and ovalbumin sensitized male Wistar rats
and changes in tension induced by angiotensinogen (Aogen) and Ang
I, acetylcholine (ACh) and terbutaline were assessed by myography.
Studies were performed in absence or presence of valsartan (blocker
of Ang II type 1 receptors, AT1), pepstatin A (a renin blocker), teprotide(angiotensin I converting enzyme inhibitor) and chymostatin (chymase
inhibitor). Results obtained from bronchi of sensitized rats after in vitro
challenge (SRCB) and normal rat bronchi (NRB) were comparatively
evaluated. The Aogen and Ang I-induce AT1 mediated bronchoconstriction.
The Emax of ACh was amplified by 15% on NR and by at least 20% on
SRCB. Ang I decreased the Emax of terbutaline by 35% on NRB and toa half on SRCB. Ang I effects were decreased by teprotide (on NRB and
SRCB) and chymostatin (only on SRCB). The Aogen had lower effects
as compared with Ang I. The pepstatin A pretreatement only partiallyreduced the Aogen contractile effects, but did not modified the Aogeninduced decreasing of terbutaline effects. Our data revealed that locally
produced angiotensins modulates the bronchial reactivity and indicatesbronchial RAS blockade as a potential antiasthmatic tool.
Key words: angiotensinogen, angiotensin I, asthma, rat.

54.SUBCLINICAL HEPATIC ENCEFALOPATHYEVALUATIONBY NUMBER CONNECTION TEST (NCT)VIORELA ENACHESCU1, VERONICA SFREDEL2, SIMONATUDORICA MICU1, C. EFREM1
IIIrd Medical Clinic, Clinical Hospital no.2
Department of Physiology
University of Medicine and Pharmacy of Craiova

12Introduction: NCT, permits serial, orientative or semiquantitative
assessment of minimal changes in intelectual capacity of the patientswith liver hepatitis or cirrhosis. Aim: To evaluate the relationship between
NCT and the Child-Pugh score of liver hepatitis and cirrhosis. Method:
42 hospitalised patients with confirmed liver C hepatitis or cirrhosis were
included. We perform a clinical, biological and ultrasound diagnosis for
all the patients (11 patients were histologically confirmed) and also two
NCT for each patient, before and after the treatment. The patients`age
ranged was from 28 to 76 years old. Results: 30 patients had ascites(71.42%) . A etiological diagnosis of liver disorders was: viral hepatitis
in 26 cases (61.90%), alcoholism in 12 cases (28.57%), both in 3 cases,
primary biliary cirrhosis in 1 case. Acording to Child Pugh classification
16 patients (38.09%) were class A cirrhosis, 20 patients (47.61%) wereclass B and 6 patients (14.28%) were class C. The mean NCT value(in seconds) was 69.85+21.17``, 142.21+92.3`` and 272.58+202``, forthe first and respectively second and third class. The mean values
were 183.26+87.19`` and 89.27``+28.2`` respectively for ascitic andnon-ascitic cirrhosis. The difference of NCT values between classes
A and B and between classes A and C were statistically significative(p<0.001). There was also significative difference between the NCT
values found in ascitic and non ascitic forms of cirrhosis. Conclusions:
NCT is important in detecting and evaluating the intellectual impairment
in patients with liver hepatitis and cirrhosis and it can also be used forthe diagnosis of subclinical forms of hepatic encefalopathy

18

55.COMPARATIVE STUDY OF THE PARAMETERS
OF SOMATOSENSITIVE EVOKED POTENTIALS IN
SPORTSMEN
DENISA ENESCU BIERU1, B. CATALIN2, D. GEORGESCU2,GERMINA COSMINA1, C. FORTAN1, C. POPESCU1, M.
GEORGESCU2, MARIA IANCAU2
1

University of Craiova, Faculty of physical Education and Sport
University of Medicine and Pharmacy, Craiova

2

Our study aims at evaluating some parameters of the somatosensitive
evoked response in sportsmen from a variety of disciplines: volleyball,handball, fencing, the investigation functionally exploring the conductionof the influx in the somatosensitive system, with a large proprioceptive
participation. The study group was made up of 18 male subjects,
aged between 16 and 24 years of age, professional sportsmen, withexperience in their chosen discipline of at least 5 years. The evokedsomatosensitive response was recorded with a MEP 150 Nihon-Kohden
device, and was obtained by stimulating the median nerve, at the levelof the radiocarpian articulation, bilateral, successive. The stimulation
was made with electric stimuli of an intensity superior by 3-4 mA to themotor threshold, with a stimulus duration of 0.2 ms and a stimulation
frequency of 3 Hz. The evoked potentials were obtained with electrodes
placed on the scalp (reference electrode was Fz) , the active electrodes
were placed counter laterally to stimulation ( C3’ and C4’ respectively) .
Extracting the evoked potential from the electroencelographic recording
was made by averaging 250-300 answers. The obtained recordings
have shown components from P14 to 45, deflections to which latencies,
amplitudes and inter-maximum point intervals (P14-N20, N20-P25)
were computed. Only the wave latencies and intracranial conduction
intervals were retained for statistical processing. The recorded results
show wave latency average values that do not show statisticallysignificant differences neither for the two stimulated members nor forthe sportsmen subgroups. Positive correlations were shown for the P1,
P3, N3, P4 waves in the case of left-right differences for the entire study
group. Right-left correlation for the sports comparison does not respect
the pattern for the entire study group.
Key words: somatosensitive potentials, sportsmen, waves
latencies

56.ASPECTS OF THE ANALYSIS OF THE EEG
SPECTRUM FOR PROFESSIONAL SPORTSMENDENISA ENESCU BIERU1, B. CATALIN2, D. GEORGESCU2,GERMINA COSMINA1, C. FORTAN1, C. POPESCU1, M.
GEORGESCU2, MARIA IANCAU2
1

University of Craiova, Faculty of physical Education and Sport
University of Medicine and Pharmacy, Craiova,

2

Our study aims at obtaining EEG patterns, representative for
sportsmen of multiple disciplines, which would characterize certain
activities. Our study group was made up of 28 subjects (18 male and
10 female), professional sportsmen/sportswomen with activity ranging
between 5 and 12 years in the disciplines of fencing, handball, volleyball.
Electroencephalographic recordings were made on 16 channels with a
Nihon-Kohden device, with the electrodes placed in a 10-20 system, theFiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
time constant being 0.3 seconds and the highcut filter 50 Hz. Activities
followed while recording the EEG were: relaxed state, closing the rightfist, closing the left fist, command to close the right fist without physicalexecution, command to close the left fist without physical execution.
After each command activity, the subject was in a relaxed state, before
performing the next command. The EEG Mapping QP-220AK wasused for spectral analysis of the EEG, and time frames without artifacts
and characteristic to each activity were selected. Spectral analysis
followed the classical frequencies of the EEG: Delta, Theta, Alpha1,
Alpha2, Beta1, Beta2, by analyzing the maximum energy frequency,
the average frequency and the median frequency. The increase of
Alpha2 activity in the areas corresponding to electrodes C3, P3, T3,
C4, P4, T4 was observed.

57.MALE INFERTILITY AND BHMT G742A
POLYMORPHISMMARIUS FLORIN FARCAŞ, FLAVIU ANDREI CSERNIK, PAVELTRIFA, TANIA CRISAN“Iuliu Haţieganu” University of Medicine and Pharmacy
BHMT (Betaine-homocysteine methyltransferase) is a cytosolic
enzyme, present in the liver and kidneys, which catalyzes the conversion
of betaine and homocysteine to dimethylglycine and methionine,respectively. Methionine is a precursor of SAM (S-adenosylmethionine),
the principal methyl donor in the process of nucleic acid, protein andlipid transmethylation. The G742A polymorphism of the gene encoding
BHMT is associated with a reduced enzyme activity and therefore cangive rise to a high level of plasma homocysteine, a condition which is
known to generate serious metabolic disturbance. We have analyzed
the frequency of the 742G→A polymorphism in patients suffering from
idiopathic azoospermia or severe oligozoospermia and in a control groupof Romanian healthy men, in order to investigate the possible associationof this polymorphism and male infertility. Using the polymerase chain
reaction restriction fragment length polymorphism technique (PCRRFLP), the genotype distribution was investigated in both patientsand controls. The genotype frequencies are: 12,7% m/m; 58,7% w/m;
28,6% w/w in the studied group compared to the control group: 13,2%
m/m; 37,7% w/m; 49,1% w/w. Statistical analysis showed there is no
association between the BHMT G742A polymorphism and male infertility(p0.056). Until this date there are several known polymorphisms ofgenes encoding enzymes of the folate pathway which are associatedwith a very broad pathology: vascular disease, neural tube birth defects
and infertility. Increasing the size of the two studied groups as well as
studying other genes of the folate metabolic pathway can contribute toa better understanding of the molecular basis of male infertility. Genetic
diagnoses techniques, by the information that they offer concerning themolecular profile of each patient can contribute to better diagnoses,
treatment and prevention of diseases.
Key words: betaine, homocysteine, folate, methylation, gene

58.HYPERTENSION RELATED RISK FACTORSMARIOARA NICOLETA FILIMON1, SMARANDA RODICA GOŢIA2,
LAURA GOŢIA2, RODICA TOROK-OANCE1
Department of Biology, West University Timisoara
Department of Physiology, University of Medicine and Pharmacy Victor
Babes Timisoara, Romania

1

2

Aim: Arterial hypertension correlation with exogenous risk factors(coffee, alcohol, cigarette, stresfull work conditions, polluted working
environment) and endogenous risk factors (dyslipidemia, obesity).
Methods and materials. The study has been made on 150 people, fromurban and rural area, 45 to 57 years old, 80 men and 70 women. There
were determined high blood pressure (systolic blood pressure) and lower
blood pressure (diastolic blood pressure). Arterial hypertension has been
established when the systolic blood pressure reached 140 mmHg and thediastolic blood pressure was more than 90 mmHg. Arterial hypertension
related risk factors were taken into consideration. Results. Among theinvestigated cases, there were 130 people who were still active (86.66%)
and 20 people who were already retired (13.66%). Investigated people
admitted that they drink coffee (28%), drink alcohol and smoke (30%from the investigated men). Dyslipidemia was found in 40 women and
30 men and they were between 51 and 57 years old. 15 women and
25 men were suffering from obesity, at the same age as the ones who
had dyslipidemia. Obesity and dyslipidemia were correlated with thearterial hypertension. Knowing the risk factors allows us to establish aprognosis concerning the evolution of hypertension and also to apply
the hypertension prophylaxis. Conclusions. Risk factors (alcohol, coffee,
cigarette, stress) contributed to the birth and maintainance of arterial
hypertension. In aged people often can be accompanied by obesity anddyslipidemia, which means a risk factor for the arterial hypertension.
Keywords: arterial hypertension, risk factors, dyslipidemia,
obesity

59. THE MECHANISMS INVOLVEDIN PHOTODYNAMIC THERAPY USINGTETRAPYRROLIC MACROCYCLESADRIANA G. FILIP1, SIMONA CLICHICI1, ADRIANA MURESAN1,
DOINA DAICOVICIU1, RODICA M. ION2, CORINA TATOMIR3,
LILIANA ROGOJAN4, DECEA NICOLETA1, DIANA OLTEANU1
Department of Physiology, “Iuliu Hatieganu” University of Medicine andPharmacy Cluj-Napoca, Romania
2
National R&D Institute of Chemistry and Petrochemistry - ICECHIM,
Bucharest, Romania
3
Oncologic Institute „Prof. Dr. I. Chiricuta” Cluj-Napoca, Romania
4
Department of Morphopathology, District Hospital Cluj Napoca

1

Photodynamic therapy (PDT) mediated oxidative stress causes direct
tumor cell damage as well as micro vascular injury. In addition, tumor
eradication arises from an acute inflammatory response featured by anincreased level of cytokines in the tumor. To improve this treatment new
photosensitizers are being synthesized and tested. Our study evaluates
the effects of PDT with 5,10,15,20-tetrakis-(methoxyphenyl)-porphyrins(TMPP) and their zinc compounds (ZnTMPP) on the tumor levels of
IL4, TNFα, MDA and glutation, on the activity of caspase-3 and MMP in

Fiziologia ‑ Physiology  2009 Supplement

19Official Journal of the Romanian Society of Physiological Sciences
Wistar rats bearing carcinosarcoma 256. Rats were randomly divided
into five groups: group 1 - no treatment, group 2 - only irradiated, group3 – 5-aminolevulinic acid (5-ALA) 250 mg/kg b.w.; group 4 - TMPP 10mg/
kg b.w, group 5 - ZnTMPP 10mg/kg b.w. The tumors were irradiated for
15 minutes with LASER light (100J/cm2, 10kHz, 685nm) 24h after drug
administration. Our results show an important increase in the levels ofmalondialdehyde (p<0,01) together with the activation of MMP 2 (p<0,05),
there is a significant correlation between these two parameters. The
activity of caspase-3 is increased in the tumor tissue, mainly whenusing TMPP for PDT, in correlation with increased lipid peroxidation.
The level of TNFα in the tumor is increased after PDT with ZnTMPP.
We may conclude that the effects of PDT with these two porphyrins are
mediated by cellular membrane damage and the induction of apoptosis
and also by the activation of the innate immune system.
Key words: photodynamic therapy, oxidative stress, apoptosis,
macrocycles tetrapyrrolic and cytokines

60.NEW TRENDS IN MODERNPATHOPHYSIOLOGYAMELIA GAMAN1, C. TAISESCU1, D. ALEXANDRU1,
R.TANASIE2
1

UMF Craiova
University of Craiova

2

Pathophysiology is an integrative discipline that prepares thefoundation for the scientific notions fundamental theoretical work in
hospital practice. We developed and won a grant from European funds,
whose general objective is to develop human resource by improving
the quality of professional training of students and teachers involvedin the educational process in Pathophysiology in accordance with newEuropean requirements. The specific objectives aiming at restructuringand modernization program of study in Pathophysiology by creating andimplementing software for practical work and the assessment of students
in educational activities to improve transparency and evaluation. Using
these modern methods of teaching / learning / assessment of students
using both the MG III, participants in the project, representing the target
group, and students in the next years, beneficiaries of the practical modern
Pathophysiology made in the current project. The project contributes toachieving specific priority axis 1-Education and training in supporting
economic growth and development of knowledge society, major field ofintervention. 1.2. Quality in Higher Education in POSDRU 2007-2013 anddevelopment of human capital, increasing competitiveness and providing
greater opportunities for future participation in the labor market a modern,
flexible by promoting innovation and expansion activities using ICT inteaching / learning / assessment in higher education, skills development
cross promoting equal opportunities, gender and discrimination. The
project is in line with the European Employment Strategy, the Integrated
Guidelines for Growth and Employment, National Program of Reforms,
the National Development Plan 2007-2013, National Strategic Reference
Framework 2007-2013.
Keywords: pathophysiology, software, teaching, learning,assessment

20

61.INTERRELATION OF GLUTATHIONE
REDUCTASE AND GLUCOSE-6-PHOSPHATE
DEHYDROGENASE ACTIVITY IN PATIENTSWITH MAMMARY TUMORSGAVRILIUC LUDMILA, GODOROJA NADEJDA, BOTNARIUC
NATALIA, VARTICIAN ANA, LISII LEONID
Department of Biochemistry and Clinical Biochemistry, USMF
“N.Testemitanu”, Oncological Institute, Chisinau, Moldova
Introduction. In patients with mammary tumors the processes ofperoxide oxidation of lipids are increased and metabolism disturbed.
The enzymic redox-system of glutathione is one of the main pathwayof antioxidative system defense in the human organism.
Purpose. Investigation of the glutathione reductase (GR), glucose6-phosphate dehydrogenase (G6PDH) activity and glutathione reduce(GSH) content in the blood plasma, leucocytes and erythrocytes in thepatients with dyshormonal hyperplasiae (DH) and mammary cancer(MC). Material and methods. Leucocytes were separated from bloodusing Boyum A. method (2001). Content of GSH and activity of enzymes
have been determine in 27 patients (17-DH; 10-MC) with SP methods(Humalyzer 2000) as was described (Gavriliuc L., 2008). The control
group included 20 adult healthy. Results. The activity of GR and G6PDH
and content of GSH were significantly decreased in the blood of the BC
patients’ in comparison with the DH patients’. The results of Spearmen
correlative analysis showed, that between GR and G6PDH in plasma(r=+0.648; p<0.025), leucocytes (r=+0.636; p<0.025) and erythrocytes(r=+0.650; p<0.025) in the BC patients’ was found a functional interrelation.
In the DH patients’ an interrelation was found in leucocytes (r=+0.578;
p<0.01) and erythrocytes (r=+0.685; p=0.025) only.

62.PREVALENCE OF METABOLIC SYNDROME
AND ITS IDENTIFICATION CRITERIA BY SEX AT
ELDERLYADRIANA GHERBON, LAVINIA NOVEANU, CODRUŢ ROMAN,GEORGETA MIHALAŞ
Department of Physiology, UMF „V. Babeş” Timişoara
Introduction: metabolic syndrome contains a group of disorders which
determinate a higher incidence of cardiovascular diseases. Objective:
study of metabolic syndrome prevalence at elderly Research design
and methods: from 438 elderly (267 female and 171 male), 191 (43.6%)(129 female and 62 male) had metabolic syndrome. The criteria used
for identification of metabolic syndrome were: abdominal circumference(above 80 cm at girls and above 94 cm at boys), glucose concentration
above 100 mg%, HDLc concentration under 40 mg% at boys and under
50 mg% at girls, blood pressure above 130/85 mmHg, triglycerideconcentration above 150 mg%. We evaluated: Lipid profile (totalcholesterol, triglyceride, HDL cholesterol, LDL cholesterol); Systolic anddiastolic blood pressure; Fasting glycemia; Abdominal circumference andbody mass index (BMI). Results: We noticed a prevalence of metabolic
syndrome at women (48.31% vs. 36.25%, p<0,001, X26.16). From thecriteria used for definition of metabolic syndrome, more frequent wereabdominal circumference (100%) (67.53%F vs. 32.46%M, p<0,001,
X2G.01), blood pressure levels (79.05%) (70.86%F vs. 29.13%M,

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Official Journal of the Romanian Society of Physiological Sciences
p<0,001, X2R.57), HDLc concentration (70.15%) (73.88%F vs. 26.11%M,
p<0,001, X2a.13), glucose concentration above 100 mg% (41.88%)(67.5% F vs. 32.5% M, p<0,001, X2 .6) and triglyceride concentration(41.36%) (70.88%F vs. 29.11%M, p<0,001, X2’.57). Conclusions: atelderly metabolic syndrome is more frequent at women, presence of
metabolic syndrome shows a higher risk of cardiovascular disease.

at a patient with organic cardiac bruits, in acute endocardites, febrile
syndrome appears on a healthy heart, which creates seriously diagnosis
problems. Fever can be the major manifestation of a miocardites orheart stroke. Fever in cardiac diseases is a very usefull medical sign
because it can make the differential diagnosis between different heart

63.PREVALENCE OF HYPERLIPOPROTEINEMIAAT ELDERLY

65.TAURINE EFFECT ON SELECTIVE ATTENTION
IN ELDERLYADRIANA GHERBON, LAVINIA NOVEANU,CODRUŢ ROMAN,GEORGETA MIHALAS

M. GIURGIU, M. GHITA, L. ZAGREAN

Department of Physiology – UMF „V. Babeş” Timişoara
Introduction: hyperlipoproteinemias are frequently in general population.
Hyperlipoproteinemias types are: hypertriglyceridemia, hypercholesterolemia,
mixed hyperlipoproteinemia and hypertriglyceridemia. Objective: study ofhyperlipoproteinemia prevalence at elderly. Research design and methods:
from 438 elderly (267 female and 171 male), 189 (43.15%) (131 female
and 58 male) had hyperlipoproteinemia. We evaluated: Lipid profile (totalcholesterol, triglyceride, HDL cholesterol, LDL cholesterol); Systolic anddiastolic blood pressure; Fasting glycemia; Abdominal circumference
and body mass index (BMI). Results: We noticed a prevalence ofhyperlipoproteinemia (TC > 200mg%, HDLc < 50 mg% at female and< 40 mg% at male, TG > 150 mg%) at women (49.06% F vs. 33.91%
M, p0,0017, X29.75). In function of hyperlipoproteinemias type, we had
hypercholesterolemia in 69,84% (n 2) cases (74,24% F vs. 25,76% M,
p<0.001, X2b,06), follow by mixed hyperlipoproteinemia in 18% (n4)cases (64,7% F vs. 35,3% M, p0,01, X25,88) and hypertriglyceridemia
in 12,16% (n#) cases (47,82% F vs. 52,18% M, p0,76, X20,09).
Conclusions: at elderly hyperlipoproteinemia is more frequent at women;
more fervent was hypercholesterolemia, which represent a major factor
for aterosclerotic cardiovascular disease.

64.FEVER IN CARDIAC DISEASESGHERTA LEONID, MANU ROXANA ELENA, MARGINEANCRISTINA
University of Medicine and Pharmacy of Craiova
Fever (also known as pyrexia, from the greek pyretos meaning
fire, or a febrile response, from the latin word febris meaning fever, andarchaically known as ague) is a frequent medical sign that describes
an increase in internal body temperature to levels above normal. The
temperature of the body is ultimately maintained in the hypothalamus.
A triggering agent of the fever, called pyrogenin, causes the releaseof prostaglandin E2, that afects the hypothalamus, which generates asystemic response back to the rest of the body, causing heat-creating
effects to match a new temperature level. Pyrogen substances can beeither endogenous or exogenous to the body. Any febrile syndrome ina patient with a cardiac disease creates problems in diagnosys andevolution. Situations in which fever may appear in patients with a cardiac
disease are: Acute Articulary Reumatism, septicemy, infectious diseases,
acute pericardites, heart stroke, bacterian subacute endocardites.
Any febrile syndrome that appears at a patient with a valvulopaty cansuggest the posibility of an existing subacute endocardites. Unlike
subacute endocardites, in which fever exists in over 95% of the cases,

Fiziologia ‑ Physiology  2009 Supplement

diseases, along other symptomes

Department of Physiology, U.M.F. “Carol Davila” Bucharest
Aging is a process that associates a decline in cognitive function,
involving frontal lobe, especially prefrontal cortex. Also, selective attention
and its underlying mechanisms (excitation, inhibition and habituation) are
impaired in normal aging. The Stroop test has been traditionally used asa measure of cognitive inhibition. Taurine is a well known neuroprotector,
being involved widely in central nervous system regulation. The aim of
this study is to investigate if taurine is involved in the inhibition process.
Materials and methods: The Stroop test was performed to a group of 10
patients, over the age of 65, without neurological and ophthalmologicalhistory. At the beginning of the study, each patient was evaluated withpsycho-geriatric assessment scale. The patients followed a 3 monthtaurine treatment protocol. The recordings were performed before taurine
administration, and after a treatment of 1 and 3 month. We evaluatedthe reaction time and error type. Stroop test and answer recordings
were made with Superlab 4.0 software. The results showed a beneficial
effect of taurine over the recorded parameters, versus a control groupthat received placebo. Ongoing studies are done to explore the durationof taurine effect
Keywords: aging, taurine, stroop, reaction time

66.BONE REMODELLING IN POSTMENOPAUSALOSTEOPOROSIS.
SMARANDA RODICA GOTIA1, CAMELIA GURBAN2, FELICIASFRIJAN2, LAURA GOTIA1, M. COJOCARU3.
Victor Babes” University of Medicine and Pharmacy, Physiology
Department, Timisoara, Romania
2
Victor Babes” University of Medicine and Pharmacy, Biochemistry
Department, Timisoara, Romania
3
Titu Maiorescu’’ University of Medicine and Pharmacy, Physiology
Department, Bucharest, Romania

1

The bone is permanently liable to a process of bone remodelling,which has a cyclic character. The resorpted bone induced by osteoclasts isequal to the formed bone, through osteoblastic action. The differentiationof osteoclasts is promoted by parathormon, calcitriol, thyroid hormones
and inflammatory cytokines (interleukin-1 and 6, tumor necrosis factor).
In the presence of the acid environment, hidroxiapatite crystals fromextracellular womb will be rendering soluble with calcium and phosphatesions released in blood torrent. In osteoporosis bone resorption begin
gradual to outrun new bone formation rhythm. At the beginning takes
place a process of focal bone decalcification realised by increased
osteoclasts activity as a result of ATP-pomp activation with H+ protons

21Official Journal of the Romanian Society of Physiological Sciences
hyper secretion forming that way an adjacent acid medium which will makesoluble hidroxiapatite microcrystals. ’’The gold standard’’ in osteoporosis
evaluation is represented by dual energy X- ray absorbtiometry method,which measures spine and/on hip bone density levels. In postmenopausal
osteoporosis serum levels of the bone making markers (osteoprotegerin,bone alkaline phosphatase, osteocalcin, estrogens) are significantly
reduced comparing the patients without osteoporosis, and bone
resorption markers levels (receptor activator of nuclear factor-ĸappa B)
are significantly increased, comparing the patients without osteoporosis.
The findings suggest that these sensitive bone markers may be used
to monitor treatment efficacy, especially within the first 6-12 months ofinitiating therapy, at a time when bone mineral density changes are still
too insignificant. The serum levels of bone markers may be useful for
osteoporosis ’’screening’’ in postmenopausal women.
Key words: bone remodeling, osteoporosis.

67.ECOPHYSIOLOGY OF GASTROINTESTINAL
TRACT. REVIEWGOTIA SMARANDA RODICA1, VERDES DOINA2, GOTIA SMARANDALAURA1, GURBAN CAMELIA3, FILIMON NICOLETA4
1
Department of Physiology, University of Medicine and Pharmacy
Victor Babes
2
Department of Molecular and Cell Biology, University of Medicine andPharmacy Victor Babes
3
Department of Biochemistry, University of Medicine and Pharmacy
Victor Babes
4
Department of Physiology, West University, Timisoara, Romania

The aim: to discuss the main aspects of environment changes impact
on gastrointestinal tract. Ecophysiology of gastrointestinal tract studies
interactions between individual digestive system and their environment,
also, contains interaction between diet, microflora and the resistanceto intestinal pathogens, food borne infections, probiotics interaction
with intestinal flora and local immune system. Gastrointestinal tract
represents a barrier between human body and external environment.
Therefore physical, chemical, biological ecosystems variation induces
structural and functional modifications of human digestive system.
The main environmental factors which assault digestive system arefood, water, parasites, microorganisms. The exponentially growing
human population requires increasing supplies of food and fiber.
Developments in biotechnology, food technology, and gene technology
lead to increased possibilities for novel and functional food development.
Physiological response to functional foods ingestion may involve thedigestive function, composition and metabolic activities of intestinal
flora, and the immune system. Plants and animals genetic modified
used as food for humans, stress can influence our body development.
The impact results can be: intestinal motility impairement, decrease ofnutrients and vitamins absorbtion, proteic malnutrition. Food aditives caninterfear with nutrients, iron, and vitamin absorbtion followed by specificdeficiency. Global climate change may affect health by modification ofnatural ecosystems and agriculture which induces effects of population
displacement, diseases induced by vector, rodent, water, and food(diarrhoeal episodes, malnutrition’ risk). Temperature and relative
humidity have a direct influence on the bacterial replication rate, andon enteroviruses survival in the environment.
Key words: environment, digestive system.

22

68.GLYCAEMIA VARIATIONS IN SALIVARY GLANDSTUMORS
GOTIA SMARANDA LAURA1, GOTIA SMARANDA RODICA1,GURBAN CAMELIA2
Department of Physiology, University of Medicine and Pharmacy Victor
Babes, Timisoara, Romania
2
Department of Biochemistry, University of Medicine and Pharmacy
Victor Babes, Timisoara, Romania
1

It has been recently revealed that type 2 diabetes is a risk factor for
tumors proliferation at different sites. The aim: the correlation of glycaemia
values with salivary glands tumors. Material: There were selected 87
patients with salivary gland tumors admitted in Maxillo-Facial Surgery
Department from Timisoara, for a period of two years. The study was
based by medical records, biological parameters and histopathologicalresults. Was noted tobacco use, as risk factor. Results: The range ofage of patients was between 45 to 85 years. The incidence of salivary
tumors was higher in men (56.32%) and urban area (70.11%). Theirtumors were predominantly benign, 76.93% in non-smoking (52 cases),and 65.38% in smokers patients (26 cases). High levels of fasting blood
glucose presented smokers patients with salivary gland cancer (118.8 ±52.89 mg/dl) compared with non-smokers (97.6 ± 11.28 mg/dl). There
was a positive correlation of glycaemia values and age in salivary benign
and malignant tumors, both in non-smokers (r 0.506), and smokers (r0.380) patients. The results of the study suggest that high levels of
glycaemia can be induced by the decrease of insulin-like growth factor
produced in normal salivary glands. Patients were advised to moreinvestigate their glycaemic metabolism in order to reveal a possible
diabetes mellitus. Conclusion: Malignant salivary gland tumors wereaccompanied with high levels of glycaemia. These findings permit anovel hypothesis concerning the association between hiperglycaemia
and salivary gland tumors.
Keywords: salivary gland, tumor, glycemia.

69.LOCAL BLOCKADE OF INTRAPULMONARY
RENIN ANGIOTENSIN SYSTEM EFFECTS ON
PULMONARY ARTERY REACTIVITYGURZU BOGDAN1, PETRESCU BOGDAN CRISTIAN2, COJOCARUELENA1, DUMITRIU IRINA LUCIANA1, VATA LUMINITA GINA1,
MARIA DINCA1, MIHAI CAMELIA ANA1, COSTULEANU MARCEL2,
PETRESCU GHEORGHE2
Department of Physiology, School of Medicine, “Gr T Popa” Universityof Medicine and Pharmacy Iasi, Romania
2
Department of General Pathology, School of Dentistry “Gr T Popa”
University of Medicine and Pharmacy Iasi, Romania

1

Our previous data confirmed the activation of local renin angiotensin
system (RAS) during the progression of lung allergic diseases. This
study examined to what extent the local blockade intrapulmonary RAS
could modified the pulmonary artery reactivity. Pulmonary artery (PA)rings obtained from normal rats (NR), ovalbumin sensitized rats (OSR)
and ovalbumin sensitized rats treated with losartan (an angiotensin
receptor AT1 blocker) (OVAL) were mounted between hooks in a wire
myograph and changes in rings tension were recorded. Losartan was

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
daily administrated by nebulization (15 minutes, 1%) in the last week ofsensitization protocol. Phenylephrine (10 µM, Phe) induced contraction
and subsequent acetylcholine induced relaxation (ACh, 10 nM – 10 µM
cumulative doses) were used for estimation of PA reactivity. The Phe –induced contractions were 1.2 times powerfully on PA ring from OSR as
compared with NR. The ACh induced relaxation of Phe – preconstricted
PA rings was significantly lower on OSR (Emax: 59.82±5.31%) thanon NR (Emax: 75.84±3.88%). On OSRL, the Phe contractile effects
were not significantly different than OSR, but ACh – induced relaxation
was significantly improved (Emax: 64.43±3.41%, p<0.05 as comparedwith OSR). These results suggested that inhalatory administration ofan angiotensin receptor AT1 blocker could have a benefic effect on
preventing the modification of pulmonary vascular reactivity induced
by sensitization and challenge.
Keywords: pulmonary artery, losartan, ovalbumin, rats.

70.THE STUDY OF CEREBRAL DOPPLER AND
CARDIO-VASCULAR PARAMETERS TO A LOT OFCONTRACEPTIVE FEMALE USERS.
GUSTI SIMONA1, ILINCA IONELA AURELIA2, GUSTI ALICE3
Department of Physiology, Faculty of Medicine, University of Medicine
and Pharmacy, Craiova
2
Department of Planning Familial, University, Piteşti
3
Department of Physiology, faculty of Physical Education and Sport,
University of Craiova

1

We have investigated clinical and paraclinical to a lot of 162
contraceptive users (contraceptives of II and III generation andinjectable), 17-30 yeara old. We have determined electrocardiogram,
electroencephalogram, systolic and diastolic blood pressure. We haveappreciated cerebral irrigation with Doppler method , using a Logidop
4, Krantzbuhler and a sonde of 4 MHz, connected to a PC. Systolicand diastolic blood pressure have been mentained in normal limits, up
to superior limit of normal to all the studied lots, prevalent to injectable
contraceptive users lot and especially after a long utilization period.
The Doppler exploration emphasized a discret reduction of cerebral
irrigation (decreaseing of systolic speed with 8%) and an increase of
cerebral circulation resistance (increase of resistance index with 11%),parameters best correlated (r=0,88) with the value of systolic and diastolicblood pressure. Electrocardiogram and electroencephalogram did notrecord pathological modifications. In conclusion, the short time usingof contraceptives do not modify the cardio-vascular parameters andcerebral irrigation; the long time using of contraceptives could determine
an increase of systolic and diastolic blood pressure and an increase of
cerebral resistance, particulary to users of injectable contraceptives.
Key words: contraceptive female users; systolic and diastolic bloodpressure; Doppler cerebral vascular exploration

71.PSEUDOMIXOMA PERITONEI- RARE MUCINOUS
TUMOR OF THE PERITONEUMADRIANA HABOR1, V. HABOR2, ANCA SIN3
Discipline of Physiology, University of Medicine and Pharmacy TarguMures
2
1st Surgical Clinic, Clinic Hospital Targu-Mures

1

Fiziologia ‑ Physiology  2009 Supplement

Discipline of Cellular and Molecular Biology University of Medicine andPharmacy Targu-Mures
3

We present the case of a 48-age old female, who was admitted
to the Ist Medical Clinic from Targu-Mures with the diagnosis: chronic
bronchitis, pulmonary emphysema, chronic cor pulmonale. The patient
was immediately hospitalized in order to decompensate the pulmonary
disease. A physical examination will often reveal dyspnea and wheezing,
face and extremities cyanosis, swelling jugular, diffuse hipersonority,
prolonged expiration, disseminated sibilant rales, increasing abdominal
girth with flank dullness at percussion, flank fixed dullness, diffuse
sensitiveness at palpation, firm hepatomegaly enlargement of the liver(about 17 cm the right lobe), high consistency, smooth surface, withrounded inferior border, gaiter edema. Ordinary investigation are to beeffectuated and the treatment concerning the decompensation of thechronic pulmonary disease is initiated (oxygen therapy, anticoagulants,
diuretics, nitrates, antibiotics, digitalis, bronchodilators, corticotherapy). A
week after being hospitalized the patient had developed colon occlusion.
Abdominal ultrasonography revealed ascitic fluid in peritoneal cavity,
between the intestinal loops, ascites with echogenic intraperitoneal
masses, some of them being attached to the visceral peritoneum. The
laparatomy revealed a mucous tumor mass which was pulled out for
detachement the intestinal loops. The histopatological investigation had
pointed out a fibrin network inside the loops where are to be found thegelatinous material, pseodomucous, some glandular fragments by abenign simple columnar epithelium, ways specific to the pseudomixoma
peritonei. We had presented this case due to the rareness of the tumor
and the imprevisible evolution (starting with the spontaneous healing
concerning the cases which are advancing gradually and lead torepeteated interventions).
Keywords- pseudomixoma peritonei, mucous tumor, fibrin.

72.CONTROLLED OXIDATIVE STRESS SYSTEMUSED TO INDUCE ENDOTHELIAL DYSFUNCTION
IN ISOLATED ARTERIESHOGAS MM, STOICA B, SERBAN IL, NECHIFOR M, SERBAN
DN“Grigore T. Popa” University of Medicine and Pharmacy, Iasi,
Romania
We designed an original system (patent subject) for controlled
generation of oxygen free radicals and tested it for applicability inevaluating the effects of oxidative stress upon endothelium-dependent
relaxation. We used a quinone, that turns into semi-quinone radical
when irradiated in UV. The latter is able to transform molecularoxygen into singlet oxygen, which then forms reactive oxygen species,
predominantly superoxide. The intensity and duration of UV irradiationof the samples were precisely controlled. We used lucigenin amplified
chemiluminescence to determine optimal quinone concentration(0.5 mM) and optimal irradiation duration. Experiments were done intriplicate, either without irradiation of the quinone solution (control) or with
irradiation for three different durations. Chemiluminescence revealed alinear relation between free radical generation and irradiation duration.
In separate experiments we applied the same irradiation protocol to 5
ml batches of Krebs-Henseleit solution (containing 0.5 mM quinone),which were immediately used to incubate rings of rat mesenteric arcadeand first order branches, mounted and equilibrated in the chamber of23

Official Journal of the Romanian Society of Physiological Sciences
a wire myograph. Using this procedure of controlled oxidative stress
we induced an endothelial dysfunction, without affecting the contractile
responses, elicited by phenylephrine and high extracellular potassium,or the endothelium-independent relaxation by nifedipine. Using inhibitionof nitric oxide synthase and cyclooxygenase we showed that thisreduction of endothelium-dependent relaxation is due to reduced NO
bioavailability, which is partially compesated by an amplification of the
EDHF component, as also shown in other studies regarding the effectsof oxidative stress upon endothelium-dependent relaxation. Supported
by Romanian Grant CNCSIS-A1222/2007-2008.
Key words: endothelium, resistance arteries, quinone,superoxide.

74.THE EVALUATION OF ANTHROPOMETRIC
AND PHYSIOMETRIC EVOLUTION TO A GROUPOF STUDENTS AND ITS CONNECTION TO THE
EXISTENCE OF CERTAIN RISK BEHAVIOUR FOR
THEIR HEALTH CONDITION

73.INCREASED ARTERIAL STIFFNESS, ENDOTHELIAL
DYSFUNCTION AND CARDIAC SIZE ARE STRONGLYRELATED TO BIOIMPEDANCE-DERIVED PARAMETERS
IN MAINTENANCE HEMODIALYSIS PATIENTSThe present study aims at identifying the presence and especiallythe spreading of unhealthy behaviour to children and young people(unbalanced nutrition, alcohol consumption, smoking) and possible
connections to their physical development. As a consequence, we
selected a group of 160 students (80 secondary school students
and 80 high school students without taking into consideration theirgender). We measured a series of anthropometric and physiometricparameters and they were asked to fill in some forms. The obtained
results emphasized that long time smokers who frequently drink alcohol
have most cranial and trunk parameters as well as physiometrical ones
higher than normal. They have no extracurricular activities and lead asedentary life. Thus, we concluded that, in the case the group of students
selected, the subjects that presented higher values of anthropometricand physiometric parameters had a strong tendency toward high risk
behaviour for their health condition.
Key words: student, development, health, behaviour.

HOGAS SM, VORONEANU L, GUSBETH-TATOMIR P, SERBAN
DN, HOGAS MM, COVIC AC“Grigore T. Popa” University of Medicine and Pharmacy, Iasi,
Romania
Cardiovascular morbidity and mortality is enhanced in chronic kidneydisease vs non-renal population, partly due to increased arterial stiffness.
In chronic hemodialysis (HD) patients we investigated the relation
between fluid status, arterial stiffness and endothelial dysfunction. We
included 63 stable HD patients (age 54.3±13.1, 54% males), without
overt CV disease. Pulse wave velocity (PWV) and augmentation index(AIx) were evaluated by applanation tonometry before the mid-week
HD sessions. Pre-HD bioimpedance parameters were measured todetermine total body water, extra- and intra-cellular water. A phase angle< 6o was previously considered reflecting extracellular overhydration.
Fluid status was evaluated based on inferior vena cava (IVC) diameter.
Endothelium-dependent (ED) and -independent (EID) vascular reactivity
were assessed by changes in Alx following sublingual nitroglycerin (NTG)
and inhaled salbutamol (SAL). PWV directly correlated with patients’ age
and dialysis vintage (r0.51 vs. r0.71; P < 0.05). On multiple regression
analysis, age was the most important predictor of PWV; the model
including age, dialysis duration and phase angle predicted 26% of PWVvariance (P0.001). Patients with a phase angle <6°, were significanly
overhydrated (larger IVC, increased ECW and lower ICW), had stiffer
arteries and greater left ventricle mass, compared to those with phaseangle >6o. Both EID and ED responses were more abnormal in patientswith a phase angle < 6o. In HD volume overload is an important contributor
to increased arterial stiffness, abnormal vasodilation and LV hipertrophy.
Bioimpedance-derived parameters are excellent discriminators for amore abnormal cardiac and vascular profile. Supported by Romanian
Grant PN2-IDEI-PCE/ID-1156/2007-2010.
Keywords: arterial stiffness; hemodialysis, endothelial dysfunction,bioimpedance, hydration status.

24

ADRIANA HUREZEANU, PETRICA BADEA, VALERIUNEŞTIANU
University of Medicine and Pharmacy of Craiova

75.CONSIDERATIONS REGARDING THE INITIAL
THERAPY OF URINARY INFECTIONSHUREZEANU DAN, DRAGONU LIVIA, ŞTEFAN ELENA
University of Medicine and Pharmacy of Craiova
There has been recently remarked an increased percent of urinary
infections from one year to another. The main reasons are related tothe beginning of the therapy before actually identifying the pathogen
agents or the causes that may exist in the pre-existent pathology of thepatient. The present study aims at evaluating the antimicrobial activityof some antibiotics that are frequently used in the treatment of urinary
infections to the patients from the Clinic of Infectious Diseases, Craiova.
As a consequence, 628 microbial strains were isolated,493 of whichwere E.Coli ones (76,11%), 56 were Enterobacter (8,20%) , positive
Gram cocci (staphylococci, enterococci )- 8,20 % and other negative
Gram cocci( BGN )-9,12 %. 78,91 % (p0,004) of them, the nalidixic
acid – 72,92 % (p9,2x10-4 ) as well as cotrimoxizole 51,63% (p2,1x10-5) were sensitive to fluorochinolone. The treatment with B-lactam
antibiotics, ceftriaxone (89, 12%) and imipenem ( 90, 14%) was also
beneficial because of their high rate of activity, presenting only small
differences between them (p0,387) .Ceftriaxone was a lot more active– OR0,22 0,13,75) than t-ticarcillin/clavulanate (60,89%). Amicacin (94,43%) was more efficient than Gentamicin (72, 11&): OR0,19(o,12;o,30).
The colistin still remains a useful solution having an efficiency of -90,87%. For infections that need parenteral therapy, colistin, amicacin,
cephalosporin antibiotics of 3-4 generation as well as carbapenemes
are the most active ones.
Key words: urinary infection, antibiotic, efficiency.

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Official Journal of the Romanian Society of Physiological Sciences

76.ASSOCIATION BETWEEN HOMOCYSTEINELEVELS AND OTHER CARDIOVASCULAR FACTORSIN PATIENTS WITH METABOLIC SYNDROME
LOREDANA HURJUI, I. HURJUI, IONELA LACRAMIOARASERBAN, VERONICA LUCA, NASTASIA GHEORGHITA, D.N.
SERBAN
UMF “Gr. T. Popa”, Iasi

77.THE IMPORTANCE OF LOW MOLECULAR WEIGHTHEPARINS ADMINISTRATED IN PATIENTS WHO
UNDERWENT ORTHOPEDIC SURGERYILEANA ION, N. CEAMITRU, CECILIA ADUMITRESI, NINELARADULESCU, CRISTINA FARCAS, CARMEN CIUFU, LOREDANAHANZUPhysiology and Pathophysiology Department, Faculty of Medicine,“Ovidius”
University, Constanta
The objective of our study is to emphasize the advantage of using
low molecular anticoagulant treatment in patients with limb fractures
who underwent orthopedic surgery. In this study, were examined patientswith fractured hips, hospitalized in the Orthopedic surgery departmentof the County Hospital – Constanta. During the whole period of
hospitalization, have been administrated to the patients, subcutaneously,
once daily, low molecular weight heparin’s (LMWH). The blood tests
were performed before surgery, immediately after it and 7 days latter.
From plasma obtained after blood centrifugation, collected on sodium
citrate, were determined Fibrinogen (FIB), Activated Partial Prothrombin
Time (APTT), Prothrombin Time (PT), and Thrombin Time (TT). From
the whole blood collected on EDTA, thrombocyte number was counted.
The obtained data were statistically analyzed and values of P < 0.05
were considered significant.
There was observed the increase of thrombocytes number andfibrinogen values in patients with hips fractures as a reactive response to
the inflammation and surgical intervention. Also, under LMWH treatment
APTT was maintained in the normal range and prolongation of PT and
TT were noticed. Because LMWH administered subcutaneously (SC)once daily are at least as effective and safe as low dose unfactionatedheparin (UFH) administered SC two or three times daily, LMWH has
become the anticoagulant of choice for the prevention of venous
thrombosis following major orthopedic surgery.
Key Words: low molecular weight anticoagulants, hips fractures,venous thrombosis

78.THE WILSON DISEASE IN FAMILY MEMBERSIOVANESCU DORINA1, SFREDEL VERONICA2, AMZOLINI
M.3,AMZOLINI ANCA2Emergency Hospital Craiova, Department of Neurology
University of Medicine and Pharmacy Craiova
3
ST. Pantelimon Hospital Bucharest

1

2

Wilson disease is a genetic disorder characterized by an excess

Fiziologia ‑ Physiology  2009 Supplementof copper in the body, caused by a deficit in the synthesis of hepatic
ceruloplasmin, followed by the deposition of this metal in the brain, liver,eyes, kidneys. Objective: Outlining the main neurological aspects of
the Wilson disease in 2 sibling patients. Material and methods: In thecase of the siblings, they have undergone several medical exams: MRI,
EEG, liver biopsy, neurologic, clinical, biochemical and eye exams. The
disease developed in the male patient with psychological disorders at
about the age of nine, followed by the extrapyramidal syndrome andsubsequently epilepsy. His sister started to show at the age of 16 thespecific extrapyramidal signs, followed by severe psychical distress with
self-injuring behavior. The neurologic examination has showed in bothpatients neurologic features leading to extrapyramidal manifestations,
tonic spasms, resting, postural, or kinetic tremors, psychical retardationand depressive symptoms in the girl. The biochemical analyses showed
low levels of ceruloplasmin in the case of both brothers, plasmatic copper
under the normal level and urinary copper over the normal limits dueto the treatment with chelating agents. The MRI exam found specificimages of cerebral destruction. Results: We have found the following
characteristics:1. Development and evolution as a neurological disease;
2. The lack of liver disease; 3. The absence of the Kayser-Fleischer rings;
4. No renal involvement; 5. The EEG symptoms are more evident in thefemale patient. Conclusions: The reported cases distinguish themselves
through predominant neuropsychiatric symptoms.
Keywords: Wilson disease, neuropsychiatric symptoms.

79.THE EFFECT OF LIPOIC ACID ON MATERNALHYPOXIA DAMAGE TO THE FETUS VISUAL BRAIN– EXPERIMENTAL STUDYJOANTA ADELA ELENA1, MICLAUS VIOREL4, CZIKER ROXANAELENA1, SECELEANU ANDREEA2, HERMAN ANDREEA3, MOCANUTEODORA1, DECEA NICOLETA1, MOLDOVAN REMUS1
Physiology Department, University of Medicine and Pharmacy ”Iuliu
Hatieganu”, Cluj-Napoca
2
Anatomy and Embryology Department, University of Medicine andPharmacy ”Iuliu Hatieganu”, Cluj-Napoca
3
student, Faculty of Medicine, University of Medicine and Pharmacy”Iuliu Hatieganu”, Cluj-Napoca
4Histology Department, University of Agricultural Sciences and Veterinary
Medicine, Cluj-Napoca
1

The effect of lipoic acid on maternal hypoxia damage to the fetus
brain and retina was investigated . Pregnant Wistar rats were equallydivided into four groups: control (C), the group treated with lipoic acid(L), the group exposed to hypoxia in barometric chamber (H) during thelast week of gestation and the group exposed to hypobaric hypoxia andtreated with lipoic acid (HL). Oxidative stress markers and antioxidant
defense were assessed in different brain areas (occipital cortex, corpus
callosum) and retina of the offspring together with the histological studyof the occipital cortex. Brain and retina malondialdehyde (MDA) levels
were significantly increased whilst glutathione (GSH) and thyol groups
were significantly decreased in the offspring of gestates belonged to
the H group compared with the C group. Lipoic acid reduced brain andretina MDA levels and increased GSH and thyol groups concentrationin the offspring of gestates belonged to L and HL groups comparedwith their corresponding control groups. Histological study showed that
intrauterine hypoxia led to neuronal damage in the offspring’s occipitalcortex. Lipoic acid treatment was found effective in preventing brain

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Official Journal of the Romanian Society of Physiological Sciences
and retina disorders caused by oxidative stress.
Key Words: hypoxia, occipital cortex, corpus callosum

80.THE RELATION BETWEEN ELECTROACUPUNCTURE,NITRIC OXIDE AND BLOOD PRESURE
RAMONA MOLDOVAN JURCĂU1, IOANA JURCĂU2, CRISTIAN
BODESCU3, DUMITRU SUPAC4
Catedra de Fiziopatologie, UMF Cluj Napoca
Spitalul Clinic de Copii, UMF Cluj Napoca
3Drd. Catedra de Balneologie,UMF Cluj-Napoca
4Drd. Catedra de Fiziologie, UMF Cluj Napoca

1

2

The relation between electroacupuncture (EA) nitric oxide (NO)and blood presure (BP) has been demonstrated by various researches.
The curative mechanism (depressor effect) of (EA) on stress-induced
hypertensive rats might be mediated by NO due to activation of
sympathetic inhibitory system and by attenuated cardiac activities. EAchanges the activity in the NO system in the brainstem of spontaneously
hypertensive rats (SHR) and the site where EA is administered is ofimportance for this effect. EA ST 36 induces neuronal NO synthase(nNOS) expression in the gracile nucleus and medial nucleus tractus
solitarius (mNTS), and enhanced nNOS-NO in the nuclei may modify
central cardiovascular regulation, which contribute to hypotensive
effects of acupuncture. NO plays an important role in mediating the
cardiovascular responses to EA “Zusanli” (ST36) through gracile
nucleus. The distributions of NOS positive nerve fibers in the ST36
area might be one of the morphological foundations of acupuncture
effect of Zusanli point. L-Arginine-derived NO synthesis appears to
mediate noradrenergic function on skin sympathetic nerve activation,
which contributes to low resistance characteristics of acupoints andmeridians. Changes of neuronal and inducible nitric oxide synthase(nNOS, iNOS) in the rostral ventrolateral medulla (RVLM) of rats andthe activation of endothelial NOS and nNOS is one of the mechanisms
through which ST36 EA reduces BP; this reduction works through thestomach meridian. Recent studies have demonstrated that the depressor
effect of EA ST36 on hypertensive rats can be reduced by microinjectionof NO blocker into ventral periaqueductal gray matter.
Key words: electroacupuncture, nitric oxide, blood pressure

81.MECHANISMS OF MUSIC THERAPY IN ANESTHESYAND SURGERYRAMONA MOLDOVAN JURCĂU1, IOANA JURCĂU2, CRISTIAN
BODESCU3, DUMITRU SUPAC4
Catedra de Fiziopatologie, UMF Cluj Napoca
2
Spitalul Clinic de Copii, UMF Cluj Napoca
3Drd. Catedra de Balneologie,UMF Cluj-Napoca
4Drd. Catedra de Fiziologie, UMF Cluj Napoca

1Music therapy is defined as “the systematic application of musicby the music therapist to bring about helpful changes in the emotionalor physical health” of the patient for the achievement in physical,
physiological, psychological, cognitive, emotional, psycho-social andspiritual goals. The principal effects of music therapy during anesthesyand surgery are: 1. clinical effects: acting on the limbic system, it will26

reduce of heart rate, mean arterial blood pressure and breathing rate;
2. neurological effects: transporting the person from BETA to THETA,
background instrumental can help the individual while in DELTA sleep,
favorise the integration of therapeutic suggestions; 3. biological effects:
hypo-metabolic response, stable hemodynamical parameters, significant
reduction in the level of ACTH, cortisol, endorphin and noradrenaline,
decreased postoperative recovery profiles; 4. drugs effects: reduction inperioperative and postoperative analgesic and antiemetic requirements,
sparing the sedative and analgesic; 5. psychological effects: reduce pain,
produce relaxation, increase the satisfaction scores overall, decrease theanxiety scores overall, influence the psychologic measures – decrease
anxiety, and increase satisfaction - during women’s cesarean delivery;
6. spiritual effects: increase serenity, hope and faith.
Key words: music therapy, anesthesy, surgery

82.RELATION BETWEEN SPINE STATIC AND
JUNIOR FOOTBALL PLAYERS SPORTIVEPERFORMANCEANAMARIA KESE1, ELENA TAINA AVRAMESCU1,2, DENISAENESCU-BIERU1, MIRELA LUCIA CĂLINA1,2, LIGIA RUS U1,2
Faculty of Physical Education and Sport Craiova, University of Craiova,
Romania2
Sport Polyclinic Craiova, Romania

1

Introduction: The spine static disorders represent very importantfactor that decrease the sportive performances, especially for football
players. Objectives: The aim of this study was to establish a relation
between spine static and junior football players’ sportive performance,
to compare the possible spine static disorders to the possible ones ofa group of non-athletic kids (witness lot) and to emphasize the kinetic
rehabilitation programme importance used to prevent and treat any spine
physical deficiencies. Material and method: We study a lot of 20 juniorfootball players and a group of 20 kids which perform sport activities onlyat school, both with a medium age of 12 years, which did not accuse any
joint or muscular symptomatology. To examine the spine static, we used
the Sammons Preston scoliometer. Results: 60% of the junior football
player presented spine physical deficiencies in comparison with the 50%of the witness lot which had the same physical deficiencies. Conclusions:
The diagnosed spine static disorders benefit of corrective measuresthat consist of kinetic rehabilitation programme, therefore, these notrepresent contra-indication for practising feat sports (football).
Key words: sportive performance, junior football players, spine
static disorders, kinetic rehabilitation.

83.USING MOUSE ADULT CARDIOMYOCYTES IN
THE STUDY OF CARDIAC PROHYPERTROPHIC
SIGNALLING PATHWAYSANGELA M. LAZAR
Departament of Physiology I, University of Medicine and Pharmacy“Carol Davila”, Bucharest, Romania
Cardiac hypertrophy, an initially adaptative remodelling of the heart
to enhanced workload to maintain cardiac function is considered to be an
independent risk factor for ischemic heart disease, myocardial infarction,

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Official Journal of the Romanian Society of Physiological Sciences
arrhythmias, heart failure and sudden death. There is an association
between ventricular hypertrophy and increased mortality, cardiac
hypertrophy itself and not elevated wall stress triggering decompensation.
The aim of our project was to develop in vitro techniques to study key
pathways that signal to hypertrophy in adult mouse cardiomyocytes.
Although studies employing this kind of cells are more significant thanother/neonatal cells, a limited number of laboratories in the world use
adult mouse cardiomyocytes, due to the complexity of the method.
Mouse cardiomyocytes are primary cells that do not multiply anymore
and are extremely sensitive to a large variety of factors. Basic conditionsof the Langendorff isolation procedure are not clearly delimited and varya lot between laboratories for adult mouse hearts. Also, the cell numberthat can be isolated from a mouse heart is extremely limitative and astandardization for the stimulation conditions regarding mouse heart
myocytes is difficult. We have developed an improved technique of
cardiomyocyte isolation that allows an extensive study on prohypertrophic
signalling pathways (CaMKIIδ , p65, IkBα, ERK). We have characterizedsome of the key signalling proteins in pro-hypertrophic calcium-dependent
pathways in control and stimulated adult cardiomyocytes.

84.CONSIDERATIONS REGARDING THE HUMANHEALTH RISK CAUSED BY POLLUTION WITHHEAVY METALS.
NINA LIOGCHII, ADAM BEGU, LORA MOŞANU, ALA
DONICA
Institute of Ecology and Geography, State University of Tiraspol, Rep.
Moldova
One veritable indicator regarding the state of environmental
components is the state of human health. The main factor, throughwhich human kind can encounter different diseases, is the air. Polluted
air causes a range of clinical manifestations and diseases; however
the effect and consequence of different atmospheric pollutants on thehuman body is hard to define. In this regard, an important factor isthe capacity to accumulate toxic elements, for instance the capacityof certain phytobioindicators (lichens and mosses) to accumulate
heavy metals dissolved in substances, aerosol. Although many heavy
metals are normally present in living organisms, in quantities that do
not exceed allowable limits, they represent and pose a risk for humanhealth. Penetrating into the body, heavy metals combine with specificproteins and cumulate in different tissues. In order to avoid exposure
to such pollutants are welcomed outdoors walks, particularly in areas
considered less polluted, sheltered from noise and intense traffic andlocated far from industrial cites. This is the purpose of recreation areas. In
this context, was evaluated the concentration of heavy metals in lichenssamples collected from certain recreation areas of Chisinau city. There
was conducted an investigation and analysis of data obtained throughthe risk of human body exposure to excessive concentrations of heavy
metals. The systematic and morphological peculiarities of the lichensspecies was determined using the microscopes MBS -10, Micmend - 5
and Heavy Metal Content Identifier using the spectrophotometric atomic
absorption method. The comparative analysis showed pollution withheavy metals, particularly with increased toxicity (Pb, Cd) in the Stefan
cel Mare şi Sfint Parc, Scuarului Catedralei and Grădia Botanica Parks.
Pollution by these chemicals, along with other environmental factors,
increases the morbidity of urban population living nearby.

Fiziologia ‑ Physiology  2009 Supplement

85.THE EFFECT OF CHITOSAN SUPPLEMENTATIONON THE OXIDATIVE STRESS IN CCL4 INDUCEDCHRONIC HEPATITIS
C. LOGIN1, ADRIANA MUREŞAN1, SIMONA CLICHICI1,NICOLETA DECEA1, DOINA DAICOVICIU1, R. MOLDOVAN1,OANA CHILIŞOIU2„Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca,
Physiology Department
2
Student, 6th year, Iuliu Hatieganu” University of Medicine and Pharmacy
Cluj-Napoca

1

Carbon tetrachloride is a liposoluble substance which can passthrough the hepatocyte membrane. Its toxicity is due to the CCl3·radical production that acts on the endoplasmic reticulum. Antioxidant
supplementation has a positive effect on this type of liver disease.
The chitosan is a natural antioxidant obtained from the exoskeleton of
the crustaceous; it has a hepatoprotective effect against the injuries
induced by CCl4, reducing malondialdehyde synthesis. We used twogroups of male Wistar rats (weight 200±10 gr). Group I (n ) received
by gavage 0.3 ml/kg CCl4 25% in sunflower oil twice a week for one
month. Group II (n ) received the same amount of CCl4 and 0.3 mg/
kg chitosan twice a week intraperitoneally. We assessed the oxidative
stress markers (malondialdehyde, carbonylated proteins) and antioxidant
defense markers (hydrogen donors’ capacity, glutathione, and sulfhydrilgroups) both from plasma and liver tissue. We identified a statisticalsignificant increase in the carbonylated proteins (p.02) accompanied
by a decrease of the hydrogen donors (p.001) and SH groups (p.04)
as compared with the initial values. We didn’t identify any significant
differences between the two groups at the end of the experiment. In
conclusion, CCl4 induces oxidative stress in plasma and liver tissue,
but the chitosan, in the used dosage, do not offer protection against
the oxidative stress induced liver injuries.
Keywords: CCl4, chitosan, oxidative stress, liver

86.RELATION BETWEEN THE INTRACELULAR
Ca2+ CONCENTRATION AND PARVOVIRUS B19
PROTEIN
A. LUPESCU2, CHIRIGIU L.3, H. KAISER1, R. KANDOLF1, T.
BOCK1, F. LANG2
Depts. Of Virusology Univ. of Tübingen
Depts of Physiology & Molecular Pathology, Univ. of Tübingen
3
Depts. Of Farmacy, UMF Craiova

1

2

Infections with parvovirus B19 may cause anemia, arthropathy,
or cadiomyopathy, hydrops fetalis and congenital anemia. Cellularmechanisms leading to signs and symptoms of parovirus infection are
illdefined. The present experiments explored the effects of viral proteins
VP1, VP2 and NS1 on cytosolic Ca2+ activity. Viral proteins linked toa pronasterone - inducible promotor were transfected into human
Retinoblastoma cells (RXR-SW) and effects on Ca2+ determined utilising
Fura-2 fluorescence. Cytosolic Ca2+ activity in presence of extracellular
Ca2+, following removal of extracellular Ca2+ and subsequent additionof pump inhibitor thapsigargin (10 µM) was similar in cells transfectedwith VP1, VP2 or NS1 before and after simulation of expression by27

Official Journal of the Romanian Society of Physiological Sciences
pronasterone. Readdition of extracellular Ca2+ in the presence ofthapsigargin was followed by a sharp increase of cytosolic Ca2+ activity
which was significantly increased by pronestarone in VP1 but not VP2 or
NS1 expressing cells. The effect in VP1 expressing cells was mimickedby phospholipase A2 product lysophosphatidylcholine (1 µg/ml) andabolished by replacement of methionine by alanine in the putative
phospholipase A2 catalytic subunit of VP1. The observations point
to the activation of host cell capacitative Ca2+ entry (ICRAC) by viral
phospholipase A2. The Ca2+ entry may contribute to the pathophysiologyof parvoviral infection.

87.MODIFICATIONS OF THE LIPIDS PROFILE AT 70
YEARS OLDER PEOPLE AFTER THE TREATMENT
WITH STATIN AND POLICOSANOLMIRCEA LUPUSORU1, DUMITRU FERECHIDE1, ANCA BADARAU1,MARIANA ARTINO1, GABRIELA LUPUSORU2, CATALINA CIORNE1,
RALUCA PAPACOCEA1, ANTOANETA PUNGA1
Department of Physiology I UMF. “Carol Davila” Bucharest
Department of Nephrology Hospital Sf. Ioan – UMF. “Carol Davila”
Bucharest

1

2

Due to increased values of triglicerides and LDL-cholesterol andto the reduce value of HDL-cholesterol, the old people (after 70 years)
present major risks of cerebral and coronary events. The present concepts
tends to more categorical hypolipemiant targets. The major imedimentsof the treatment are: the increased risks of adverse reactions at highdoses of statin and also the insufficient reduction of LDL-cholesterol
with the existent hypolipemiant drugs. Therefore, the study and theimplementation of new treatment schemes with minimal adverse
effects represent the actual targets. The prospective, open-study was
made in a single center and it lasted 6 months. The lipids profiles of 40
patients (with ages between 70 and 85 years) treated with simvastatin
or simvastatin-policosanol were observed in correlation with different
biohumoral parameters, hepatotoxicity markers and with the possible
appearance of acute coronary events. The patients were chosen froma Centre of Attendance for old people. The results are superposable tothe existent actual data, but there are studies which remarke that, after
the administration of policosanol, the lipids profile has the same value
as in those cases where the placebo method was use. So, we studies
the effects of the combined treatment with simvastatin-policosanol, andthe results, concerning the lipids profile, hepatotoxicity and appearanceof acute coronary events, were much better in this case.
Keywords: old people, lipids profile, statin, policosanol

88.HEART RATE VARIABILITY IN SHORT RUNSOF YAW ROTATION STIMULIADRIAN MACOVEI, DRAGOS VLAD, DRAGOS POPESCU

minutes. ECG was recorded. RR intervals were measured manually andinput into an analysis program. Time domain and frequency domain (fastFourier transform) were performed. Results: In time domain analysis
8 subjects scored good (with RMSSD under 25 ms and PNN50 under
5.5 %). In frequency domain only six subjects scored good (adequate
HF/LF ratio). ECG were sampled into 30 seconds intervals according torotation phase and analyzed once more. The brief result are: 4 subjects
had a significant increase of HF component in overtreshold interval (2were concordant with time domain analysis (mean RMSSD 54.8 ms)
and two were not concordant (mean RMSSD 35.3 ms)); 2 subjects had
increased HF in overtreshold and decrease in high speed (concordant
with mean RMSSD 18 ms); 2 subjects had marked increase in HF (withHF/LF equalization) for overtreshold and high speed (one concordant
with RMSSD of 32.2 ms and one not concordant with RMSSD of 14.6
ms (for high speed)); 2 subjects had inconclusive results (no trend could
be established). Discussion: There seem to be a change in heart ratevariability concordant with normal rotational stimuli. High speed stimuli
induce an opposite change. Correlation with autonomic regulations inregard with known data will be discussed in detail. Error sources will beanalyzed. Conclusion: Rotational stimuli influences heart rate variability,
but the magnitude and mechanism is not clear.
Key words: heart rate variability, yaw rotation, autonomic

89.ASPECTS OF VESTIBULAR PHYSIOLOGYINVOLVED IN SPATIAL DISORIENTATION IN
FLIGHTADRIAN MACOVEI, DRAGOS VLAD, DRAGOS POPESCUNational Institute of Aerospace Medicine, Bucharest, Romania
Introduction: Spatial disorientation has a high impact in flight safety.
Materials and methods: Common illusions impacting flight performanceand their physiological explanation will be presented. We add personal
experience with our flight simulator, regarding the perception of lean
illusion. Results: Most subjects (44 of 51) perceive illusion well andrespond as expected. Difference between them is influenced byexperience (p=0.06). Discussion: Limitations of current technologies tosimulate vestibular illusion. Conclusion: Vestibular physiologic response
is key to some important spatial disorientation events.
Key words: spatial disorientation, vestibular habituation,
simulator

90.THE ANGIOTENSINS VASOMOTOR EFFECTS
ON RAT RENAL ARTERIESMARANDUCA MINELA AIDA, DUMITRIU IRINA LUCIANA, VATA
LUMINITA GINA, MARIA DINCA, MIHAI CAMELIA ANA, GURZUBOGDAN, PETRESCU GHEORGHE

National Institute of Aerospace Medicine, Bucharest, Romania

Department of Physiology, University of Medicine and Pharmacy “Gr
T Popa” Iasi

Introduction: Heart rate variability has been used to measurevestibulo-autonomic regulation with various results. Material andmethods: We used 10 subjects scanned for any vestibular andneurological problems. We exposed them to a rotatory profile, eyes
closed, clockwise, at subtreshold, overtreshold and high speed for 2.5

Published data revealed that Ang II could have either vasoconstrictor orvasodilatator effects depending on the species or experimental conditions.
The present study examined the vasomotor effects of angiotensinogen(Aogen), Ang I and Ang II on rat renal artery rings (RAR) using two
experimental models. In the first one, the different doses of Aogen,

28

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
Ang I and Ang II was administered before cumulative administrationof phenylephrine (10nM - 10microM, Phe). In the second experimental
model, the RAR were precontracted with Phe (10microM) and therelaxant responses to cumulative concentration of Aogen, Ang I and Ang
II (1nM – 10microM) were subsequently studied. The experiments werecarrying out in the absence and in the presence of losartan (10microM,
LOS) or PD123319 (10microM). The pretreatment of RAR with Ang I(100nM) or Ang II (10nM) potentiated the Phe – induced contractile
effects, by an AT2 – mediated mechanism. High doses of Ang II (1or 10 microM) reduced the Emax of Phe – induced contractions withmore than 20%; this Ang II effects were blocked by LOS pretreatment.
All studied angiotensins had relaxing effects. The order of potency
was: Ang II > Ang I >> Aogen. The relaxant effects of Ang I and Ang II
were decreased by PD123319 pretreatment but were not significantly
modified by endothelial removement. In conclusion, the angiotensins
could have either AT1 – mediated contractile effects or AT2 – dependent
but endothelium – independent relaxing effects.
Key words: angiotensinogen, angiotensin I, angiotensin II, renalartery.

91.BREAKAGE RESISTANCE OF THE FIBRIN CLOT INSUBJECTS WITH HYPERCHOLESTEROLEMIA
H. MATCAS1, VERONICA SFREDEL2, SIMONA GUSTI2Emergency University Hospital, Bucharest
Department of Physiology, University of Medicine and Pharmacy,
Craiova1

2

Our purpose was to test if the breakage resistance of the fibrin
clot is influenced by high cholesterol levels. Material and method: wehave determined the breakage resistance of the fibrin clot in a groupof 45 elderly patients, with an average of 61,4 years old, 19 females
and 26 males, whose cholesterol level was over 250 mg/dl in two
consequent determinations. These measurements were performed on
venous integral blood. Simultaneously, we investigated the fluid clotting
equilibrium by the acknowledged temporal methods: PT (protrombintime), INR, APTT and the fibrinogen concentration in the plasma. The
control group consisted of an equal number of subjects, whose age andsex ratios were comparable with the experimental group, but whose
lipidic parameters were within normal limits. Results: in the control
group, the fibrinresistometric values had an average value of 289,3 FU,
within normal limits for this age group. The cholesterol values of thepatients with hypercholesterolemia were increased with 18,9% in thegroup whose cholesterol was between 250-300 mg/dl, and increased
with 23,4 % in those cases where cholesterol values were over 500
mg/dl. PT, INR, APTT were normal, and fibrinogen levels registeredslight increases in patients with cholesterol level over 500 mg/dl.
Conclusions: While the temporal tests of haemostasis had normal values,
the resistance to breakage of the fibrin clot had significant increases,
indicating a tendency to hypercoagulability and thrombosis in subjects
with hypercholesterolemia. We believe the presented method proves tohave an increased sensitivity in determining thrombosis risk compared
to conventional tests for evaluating haemostasis.
Key words: dyslipidemias, thrombosis, resistance, fibrin clot.

Fiziologia ‑ Physiology  2009 Supplement

92.THE INFLUENCE OF HYPOLIPEMIANT TREATMENT
ON BREAKAGE RESISTANCE OF THE FIBRIN
CLOT.
H. MATCAS1, VERONICA SFREDEL2, SIMONA GUSTI2Emergency University Hospital, Bucharest
Department of Physiology, University of Medicine and Pharmacy,
Craiova1

2

Objectives. In previous studies we have discovered that the breakage
resistance of the fibrin clot increases in dyslipidemias, indicating
thrombosis risk. Our purpose is to test if hypolipemiant treatment
modifies this parameter, and if it consequently influences haemostasis
parameters. Material and method. We have measured the breakage
resistance of the fibrin clot in 22 patients with hypercholesterolemia
at the beginning of the treatment, 1 month and 3 month after statin
therapy. These measurements were performed on venous integral blood.
Simultaneously, we investigated the fluid clotting equilibrium by theacknowledged temporal methods: PT (prothrombin time), INR, APTT and
the fibrinogen concentration in the plasma. Results. The normal range ofthe method: 200-300 FU. Mean values of the breakage resistance of the
fibrin clot were 389,2 FU before administrating statins, 382,5 UF after 1
month of treatment and 375, 1 UF after 3 months of treatment. It can benoticed that after 3 months of treatment, the decrease of the breakage
resistance of the fibrin clot is of 3,7%, insignificant to correlate withstatin therapy for this haemostasis param. PT, INR, APTT were normal,
and fibrinogen concentration had slight increases in those patients withcholesterol levels over 500 mg/dl. On average, the cholesterol values
after 3 months decreased from 411 mg/dl to 250 mg/dl. Conclusions.
Despite a significant decrease in the cholesterol levels, the breakage
resistance of the fibrin clot had small values, which were insignificant.
This could be the result of the already present vascular lesions which
continue to maintain hypercoagulability.
Key words: resistance, fibrin clot, dyslipidemias, thrombosis,

93.MODIFICATION OF SERUM LIPID PROFILE IN
DIABETIC PATIENTSRODICA MATEESCU1, CLAUDIA BORZA2, GABRIELA NEGRISANU3,
CORINA SERBAN2, LELIA SUSAN4
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of Physiology
2
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of Pathophysiology
3
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of 3rd Internal Medicine
4
University of Medicine and Pharmacy “Victor Babes”, Timisoara, The
IVth Medical Clinic

1

Aim: Impaired lipid metabolism resulting from uncontrolled
hyperglycemia is implicated in cardiovascular complications in diabetes
patients. The purpose of this study was to compare serum lipid profile
between a group of diabetic patients and a group of non-diabetes
patients. Material and methods: The study was performed on 268
diabetic patients (100 men and 168 women, aged between 45-75
years) and 250 non-diabetic subjects (92 men and 158 women, aged29Official Journal of the Romanian Society of Physiological Sciences
between 45-79 years) hospitalized between January 2006 and January
2008 in the IVth Medical Clinic of University of Medicine and Pharmacy
Victor Babes Timisoara. All patients underwent complete clinical andparaclinical exams. We determined in all patients: weight, height, bloodpressure, total cholesterol (TC), triglycerides (TG), HDL-cholesteroland LDL-cholesterol. LDL-cholesterol was calculated with Friedewald
formula. Results: The prevalence of isolated hypercholesterolemia(TC ≥ 240mg/dL, TG < 200 mg/dL) was not significantly different in
non-diabetic subjects (21.8%), patients with type 1 diabetes (19.3%)
and patients with type 2 diabetes (22.3%). The prevalence of combined
hyperlipidemia (TC ≥ 240mg/dL, TG ≥ 200 mg/dL) was higher in type2 diabetes patients (20.4%) compared with non-diabetic patients(3.8%) and patients with type 1 diabetes (12.7%). The patients withdiabetes had low HDL-cholesterol (65.9% with type 1 and 56.8% with
type 2) comparative with non-diabetes subjects. Conclusion: The
most frequent lipid disorders associated with diabetes mellitus werecombined hyperlipidemia and low HDL-cholesterol. First line of the
management of dyslipidemia in diabetic patients with lipid disorders islifestyle intervention and glucose control.
Keywords: lipid profile, type 1 diabetes, type 2 diabetes

94.THE PREVALENCE OF RISK FACTORS ANDSYMPTOMS IN TYPE 2 DIABETIC PATIENTSRODICA MATEESCU1, CLAUDIA BORZA2, GABRIELA NEGRISANU3,
CORINA SERBAN2, LELIA SUSAN4
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of Physiology
2
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of Pathophysiology
3
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Department of 3rd Internal Medicine
4
University of Medicine and Pharmacy “Victor Babes”, Timisoara, The
IVth Medical Clinic

1

Aim: A great number of type 2 diabetic patients present one ormore symptoms by the time of diagnosis. Our objective in this study
was to evaluate the prevalence of risk factors and symptoms in recently
diagnosed type II diabetic patients. Material and methods: This crosssectional prevalence study was performed between March, 2008 and
September, 2008, in the IVth Medical Clinic of University of Medicine andPharmacy “Victor Babes” Timisoara. The study group included 220 type
II diabetics whose disease had been diagnosed within one year prior
to the start of our study. The collected data included medical history,
physical examination and clinical tests. Height and weight of each patientwere measured and body mass index (BMI) was calculated for eachpatient. Results: Of the 110 patients studied, 48 were men (43.63%)
and 62 were women (56.36%). The mean age of the patients was
58.8 years at the time of diagnosis. 63.5% of the patients complainedof weakness and fatigue and 19.2 % mentioned a history of transient
blurred vision. 55% of them presented: polyuria, polydypsia and weightloss. 48% of the patients had a history of diabetes in their first-degree
relatives. 72% of patients had a BMI equal to or greater than 25 and
40% of them had a sedentary life. Conclusion: Considering the big
numbers of symptoms that affect quality of life of the diabetic patientsare necessary new screening program that may help us to diagnose
type II diabetes earlier and control it more effectively.
Keywords: type II diabetes mellitus, BMI, symptoms, risk factors

30

95.STUDY REGARDING CORRELATION BETWEENIN VITRO REACTIVITY PARAMETERS OF INTERNAL
THORACIC ARTERY AND MARKERS OF ENDOTHELIALDISFUNCTION AND ATHEROSCLEROSISMIHALAS GEORGETA1, NOVEANU LAVINIA1, SAVOIU–BALINTGERMAINE2, FIRA–MLADINESCU OVIDIU2, GHERBONADRIANA1, ANDOR MINODORA3
UMP “V. Babes Timisoara - Department of Physiology
UMP “V. Babes Timisoara - Department of Physiopathology
3
UMP “V. Babes Timisoara - Department of Medical Semeiology II

1

2

Introduction: Angiographic studies performed after aorto-coronary
by-pass intervention showed a declining response of artery graft inendothelial dependent vasodilation. Objectives: (1) organ bath studyof internal thoracic artery reactivity and (2) study regarding correlationof plasmatic levels of lipid fractions, endothelin - 1, ox – LDL and
hsCRP, previously measured in patients with aorto-coronary by-passinterventions. Material and methods: maximal endothelial – dependent
vasodilating response to adenosine (10-4M) and endothelial – independent
response to sodium nitropruside (10-4M), was evaluated on 32 rings ofinternal thoracic artery. Fragments of artery were taken during aortocoronary by-pass in patients with ischemic heart disease confirmed bycoronarography. Results: Maximal vasodilating response to adenosine
was negatively and significantly correlated with total cholesterol (r - 0.60,
p 0.003), LDL – cholesterol (r - 0.66, p 0.004), endothelin – 1 (r - 0.61,
p 0.001) and ox – LDL (r -0.62, p < 0.001). There were no significant
statistical correlations with plasma hsCRP (r - 0.32, p 0.06). Conclusions:
Results regarding correlation between maximal endothelial – dependent
vasodilating reasponse of internal thoracic artery and markers ofendothelial disfunction and atherosclerosis, in patients with aorto-coronary
by-pass, may represent the basis for future studying of factors involved
in arterial graft patency.
Key words: internal thoracic artery, adenosine, cholesterol, endotelin-1,
ox–LDL, hsCRP.

96.ELECTROPHYSIOLOGICAL ASPECTS OF GALVANIC
SKIN RESPONSE DURING MODERATED PHYSICALEFFORT: SEX AND DISEASE COMPARISON
CLAUDIU MIRESCU, TRAIAN BOCU, RAMONA HANDA
University of Medicine and Pharmacy, “Iuliu Hatieganu”, Cluj-Napoca
Introduction. The galvanic skin response - GSR is a measurement
concerning the resistance of the skin to the passage of electric
current. It is determined especially by the superficial stratum of theskin and it is influenced by the activity of the sweat glands. The GSR
is an electrophysiological measurement which could reveal important
information about the state of the body. The aim of the study was toevaluate the correlation between the GSR and moderate physicaleffort. Material and methods. We collected data from 60 participants.
They were subjected to a moderate physical effort of 25 Watts using
an ergonomic bicycle, for 50 seconds. During this time the variation
GSR was measured. Also, the GSR was recorded 10 seconds before
the effort and 10 seconds after the effort. Subjects were applied the

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
Ruffier test, to investigate the cardiovascular adaptation to effort.
Results. It was found that during the rest periods, GSR has remainedroughly constant. With the onset of effort, GSR decreases rapidly andlinear in the first 10 seconds, then remains constant until the end of theexperiment. Dropping the GSR during the effort has been made withan average slope of -5.81+/-12.32. There was no correlation with the
Ruffier index. Discussions. GSR decrease is probably due to the rapid
reaction of the sudoripary glands, which are filled with secretion fluid.
Abruptness with which the GSR decreases depends on the reactivityof the sudoripary glands, which cannot be influenced by the degree of
cardiovascular adaptation to effort.

97.Pathogenesis of brain injury in acuteischemic stroke
Smaranda Mitran1, Valerica tudorica2,Iulia tudorascu3,
Badea daniela1, maria vrabete3
The Physiology Department of the University of Medicine and Pharmacy,
Craiova, Romania
2
The Neurology Department of the University of Medicine and Pharmacy,
Craiova, Romania
3
The Pathophysiology Department of the University of Medicine andPharmacy, Craiova, Romania

1

An ischemic stroke is the sudden and permanent death ofbrain cells that occurs when the flow of blood to a part of the brain
is blocked and oxygen cannot be delivered to the brain. All aerobic
tissues suffer injury when they are subjected to ischemia/reperfusion.
The pathophysiological mechanisms leading to neuronal damage after
ischemic stroke are complex and multifactorial. Regardless of themechanism of appearance, ischemia/reperfusion initiates a series of
events that ultimately lead to neuronal damage and death. Recently,
special attention has been paid to the cellular mechanisms determining
stroke appearance and development. It is known that the reduction ofblood flow leads to a reduced production of high-energetic phosphates.
Such an energetic failure causes membrane depolarization anduncontrolled release of excitatory amino acids such as glutamate into
the extracellular space (excitotoxicity). Glutamate acts upon different
types of receptors, for example, NMDA (N-methyl-d-aspartate) and AMPA
(α-amino-3-hydroxy-5-methylisoxasolepropionic acid), which overload
neurons with calcium. The enhanced intracellular calcium level, in its
part, initiates a number of key events: nuclease activation, conversionof xanthine dehydrogenase to xanthine oxidase, release of excitatoryneurotransmitters, and iron-mediated lipid peroxidation. The level ofa number of cytokines is increased after stroke (tumor necrotic factor(TNF)-α, interleukin (IL) 1 and interferon-γ) and cytokine up-regulation
is a key stimulus for activating inducible NOS (iNOS). The last leads toa sustained production of nitric oxide (NO). NO is thermodynamicallyunstable and tends to react with other molecules, thus causing oxidation,
nitrosylation, or nitration of proteins with the concomitant effects on
many cellular mechanisms.
Keywords: ischemia, reperfusion, brain injury.

Fiziologia ‑ Physiology  2009 Supplement

98.Aspects of oxidative stressin cerebral ischemia based on
experimental models
Smaranda Mitran1, Tudor- adrian balseanu1, veronica
sfredel1, MARIUS CRISTIAN NEAMŢU2, maria iancau1
The Physiology Department of the University of Medicine and Pharmacy,
Craiova, Romania
2
Faculty of Midwives and Medical Assistance, Department of Normal andPathologic Physiology University of Medicine and Pharmacy Craiova

1

On the basis of ischemia/reperfusion models, evidence has been
accumulated for the role of free radicals in the pathogenesis of ischemic
lesions. In these models, to demonstrate free radical generation, compounds
have been used that are capable of interacting with radicals to generate
stable products, which could be registered. Experimental studies using a
microdialysis technique have demonstrated hydroxyl radical production
in experimental ischemia/reperfusion. Since antioxidants are consumed
in reactions with free radicals, the measurement of their concentration or
activity is used as an indirect marker of the oxidative stress severity. A
reduced level of α-tocopherol, ascorbic acid, and ubiquinones has beenfound in the ischemic center after 30 min ischemia in rats. In a primate
model, after cerebral ischemia, a reduced level of ascorbic acid has
been reported in the right basal ganglia 2 h post ischemia. Ten minutes
after reperfusion, Katz et al. have determined a significant decrease in
the level of ascorbate, glutathione, total thiols, and α-tocopherol in the
hippocampus in an experimental model. There are also data on a global
elevation of brain SOD activity following forebrain ischemia in rats or theincrease of the level of both cytosolic CuZn SOD and mitochondrial Mn
SOD after transient forebrain ischemia. Genetically modified animals
are also a useful way of evaluating the molecular mechanisms of injury
during transient and acute ischemic disturbances in brain circulation.
The accumulating results with the models show that oxidative stress isinvolved in cell death after cerebral ischemia/reperfusion.

99. IN VIVO AND IN VITRO EFFECTS OF SSDNASWCNT.
T. MOCAN1, S.CLICHICI1, A. FILIP1, AR. BIRIŞ², D. LUPU²,
Ş.SIMON², N. DECEA1, L. MOCAN3, M. MAHMOOD4, AS BIRIS4,
A. MUREŞAN1
Department of Physiology, “Iuliu Hatieganu” Univ of Medicine andPharmacy Cluj-Napoca, Romania
2
National R&D Institute of isotopic and Molecular Technologies, ClujNapoca, Romania
3
Nanomedicine Centre, ”O.Fodor” Emmergency Hospital, Cluj-Napoca,
Romania
4
Nanotechnology Centre, University of Little Rock, Arkansas, USA

1

Introduction: SWCNT recently represent a material of extensive
interest due to their promising physical and chemical structure. Many
possible clinical applications have been proposed, including diagnosis,
drug delivery and photothermal therapies. However, their toxicity is
still under debate. Our aim was to evaluate the in vitro as well as invivo toxicity of ssDNA-SWCNT. Material and Method: ss-DNA-SWCNT
water solution was obtained through 7 hr sonication. NaCl was added31

Official Journal of the Romanian Society of Physiological Sciences
up to the concentration of 0.9%.In vitro toxicity was assessed on HeLa
cell line using Tripan Blue protocol. Optical microscopy was performed
to for cell shape, dimensions and viability assessment. In vivo effects
were measured in Wistar rats by dynamic measurement (3 hrs, 6 hrs,
24 hrs) of malondialdehyde, carbonylate proteins, hydrogen donor
ability (HD), sulfhydryl groups and gluthation blood levels.Results: In
vitro exposure induced decrease of cell viability as well as cell shapeand dimensions alterations. Dynamic decrease of HD blood levels wereobtained after in vivo exposure, with significant differences(p<0.05)
after 24 hrs. Conclusions: Our data come as evidences of SWCNT
citotoxicity and support the idea of oxidative stress pathways implicationin their toxic effect.
Keywords: SWCNT, citotoxicity, oxidative stress, in vivo, in vitro

100.THE STRESS REPERCUSSIONS THATINFLUENCE THE FORMATION AND KEEPING
OF THE MEMORY VESTIGE TO THE CHILDREN
OF 5 – 6 YEARS OLD
LORA MOŞANU-ŞUPAC, NINA LIOGCHII, CENAŞA VIORICA
Tiraspol State University, Chişinău, Institute of Ecology and Geography,
MSA
Nowadays, Psychophysiology and transpersonal psychology ismore and more preoccupied to penetrate into the abyssal dynamicof the psychic, and for this a great importance has the understanding
and explanation of memory processes, especially the formation andkeeping of the memory vestige in childhood. Actually, the specialists
are making many investigations referring to behavior and cognitive
problems. The same happen in the area of stress. However, the results
about the realization of these psychological functions, at the action ofvarious stress’ factors through which children pass in different conditionsof activity are not completely clear. The research’ purpose was toinvestigate stress’ repercussions in the formation of memory vestige tothe children in the comfortable conditions and in conditions of relative
stress. The quantitative signs of attention and memory, according towhich memory vestige was investigated, was established by applying
Burdon-Rjician- Strula Test, modified, standardized and adapted to thenecessary conditions by the workers of Psychophysiology laboratoryof MSA (1996). The test was effectuated for five times. First threetimes at the interval of one day, the fourth was on fifth day and thelast test on eighteenth day. The researches that were done allow us tounderline those specific features, that are manifesting in the formationand keeping of the memory vestige in estimated conditions. From the
beginning it was established a large dispersion of the children in thegroups at the initial steps of the test, in condition of relative stress andtheir domination in decreased groups, so that closer to the ending of
the test they reached higher features, in comparison with the conditionsof relative comfort. Grace to the obtained results we can say that theconditions of relative stress contribute to distinguish individual typical
peculiarities of memory, in case of 5 – 6 years old children and they
can be considered as a factor that favor the formation and keeping of
memory vestige to children with high stress resistance.

32

101. ELECTROMYOGRAM AND MECHANOMYOGRAM
CHANGES IN FATIGUED MUSCLE DURINGSUSTAINED CONTRACTION IN SPORTSMENDANIELA MOTOC, RAMONA BURLACU, CECILIA AVRAM,SORIN TALPES, FRANCISC SCHNEIDER
Department of Physiology, Faculty of Medicine,West Univeristy ”Vasile
Goldis“ Arad
Summary: In the surface electromyogram and mechanomyogram,the electrical and mechanical activities of recruited motor units are
sumated. The intensity and duration of muscle contraction and muscle
fatigue influence the electrical and mechanical properties of the active
motor units. The aim of this study was to evaluate fatigue-inducedchanges in the electrical and mechanical properties of motor units,
using the analysis of surface electromyogram and mechanomyogram.
The electromyogram and mechanomyogram were simultaneously
recorded in sustained submaximal isometric contractions (handgrip), withsurface electrodes and hand-dynamometer transducers, from thirty one
sportsmen. We have followed the changes in the maximum amplitudeof the signal and the force of contraction. From the time and frequency
domain analysis of the signals, the integrated electromyogram andmedian frequency of power spectrum were calculated. The conclusions
were consistent in validating the use of these parameters in evaluationof neuromuscular fatigue.
Keywords: surface electromyography, mechanomyogram, sustained
isometric contraction, maximum amplitude, force of contraction, integrated
EMG, median frequency, muscle fatigue.

102. PREVENTIVE EFFECTS OF ALPHA-TOCOPHEROLON HIV PROTEASE INHIBITORS-INDUCEDATHEROSCLEROSIS
Adelina MUNTEANU VLAD, ANGELO AZZI and JEAN-MARCZINGG
UMF ”Carol Davila” Bucuresti Departament of Physiology II
Therapies with antiretroviral protease inhibitors (ARPI) are correlatedwith a higher risk for dyslipidemia, hypercholesterolemia, and atherosclerosis.
Recent studies led to the hypothesis that upregulation of CD36 scavenger
receptor responsible for the uptake of oxLDL is critically involved. The
aim of this study was to establish whether alpha-tocopherol can reduce
CD36 scavenger receptor overexpression occurring after treatmentof monocytes with the ARPI ritonavir. We show here that treatment
of THP-1 monocytes with ritonavir increases total protein and surface
expression of CD36; however, only weak changes are observed at themRNA level, suggesting that CD36 overexpression occurs mainly at
the posttranscriptional level. Concentrations of ritonavir that upregulate
CD36 expression inhibit proteasome activity in THP-1 cells, indicating
a possible regulatory role of the proteasome in CD36 overexpression.
alpha-Tocopherol efficiently normalizes CD36 protein overexpression
after ritonavir treatment and reduces oxLDL uptake. Furthermore, in
THP-1 monocytes, alpha-tocopherol reverses the proteasome activity
inhibited by ritonavir. Therefore, we can conclude that an increased
CD36 protein expression in THP-1 monocytes induced by ritonavir
can be normalized by alpha-tocopherol and these effects are involving

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
modulation of proteasome activity.
Key words: atherosclerosis, CD36 scavenger receptor, alphatocopherol, proteasome, monocytes.

103. EXPERIMENTAL STUDY REGARDING THE
EFFECTS OF HYPOBARIC HYPOXIA ON SOMEANTIOXIDANT ENZYMES
A.MURESAN1, S.SUCIU1, S.GHIBU1, D.DAICOVICIU1,
I.D.POSTESCU2, C.LOGIN1
I.Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca,
Romania2”I.Chiricuta” Oncological Institute, Cluj-Napoca, Romania

1”

Background: It is generally accepted the oxygen reactive species
involvement in both physiological and pathological processes. Highaltitude hypoxia may induce oxidative stress in humans. Aim: The
aim of the present study was to investigate the effect of polyphenols
on antioxidant enzymes in rats exposed to high altitude. Materials
and methods: 30 male Wistar rats, divided in 3 groups, were used in
the experiment: group I – controls, Group II – rats exposed to 5500
m simulated altitude in a baric chamber for 24 hours, group III – rats
exposed to 5500 simulated altitude and which were administered 50 mg/
kg body weight polyphenols immediately after high altitude exposure.
Rats were sacrificed 1 hour after altitude exposure and blood samples
were obtained. In these samples superoxide dismutase (SOD), catalase(CAT) and glutathione peroxidase (GPx) peroxidase activity were
measured. Results: SOD levels in erythrocyte lisate was found to belower (6.23±0.36 U/mg) and CAT levels higher (5.95±0.7 U/mg) in rats
exposed to hogh altitude; polyphenols treatment returned the levelsof both enzymes (SOD 9.4±1.04 U/mg; CAT 4.94±0.2 U/mg) close
to those obtained in controls (SOD 9.31±1.04 U/mg, CAT 4.94±0.24U/mg). regarding GPx, its activity following high altitude exposure(0.035±0.006 U/mg) was similar to that found in controls (0.04±0.009
U/mg); polyphenols significantly improved GPx levels (0.205±0.2 U/mg).
Conclusion: Polyphenols seem to prevent the changes in antioxidant
enzyme activity induced by high altitude exposure.
Key words: hypobaric hypoxia, antioxidant enzymes,
polyphenols

104. OXIDATIVE STRESS EXPLORATION DEPARTMENT.
ACHIEVEMENTS. PERSPECTIVES.
ADRIANA MURESAN
I.Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca,
Romania”105. EXPERIMENTAL PHARMACOLOGIC THERAPIESIN IMPROVING DEMYELINATED AXONS CONDUCTINGIN MULTIPLE SCLEROSISMARIUS CRISTIAN NEAMŢU¹, MARIA IANCĂU2, ELENA TAINAAVRAMESCU3, LUCIAN RADU NEAMŢU4¹University of Medicine and Pharmacy Craiova, Faculty of Midwives

Fiziologia ‑ Physiology  2009 Supplement

and Medical Assistance, Department of Normal and Pathologic
Physiology
2
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Normal Physiology
3
University of Craiova, Faculty of physical Education and Sport,
Department of Human Anatomy
4
University of Medicine and Pharmacy Craiova, Faculty of General
Medicine, Department of Human Anatomy
Since the transmission of electrochemical messages between
central nervous system and the body is interrupted in multiple sclerosis
there are necessary certain therapies to improve the propagationof nervous impulses, particularly to prevent the blocking of nervous
impulse conduct in demyelinated axons. Due to the fact that theyregister anomalies in pothasium activity, the researchers have been
studying the drugs which block pothasic channels, influencing thus
directly nervous impulse propagation by favouring the increase of Na
exchanges and the decrease of initiation threshold of the action potential.
Certain studies on a low scale have demonstrated that the derivativesof a medicine called diaminopyridin have temporary improved the sight,
coordination and strength of a patient suffering both from visual symtoms
and temperature hypersensitivity. Good knowledge of heterogenous
spatial distribution of rapid K+ channels and their location beneath
myelin stratum has led to the hypothesis of using the blocking agent
4-AP to prevent conducting blocking. This approach argument is givenby the fact that blocking these channels should extend the duration of
the action potential by decreasing repolarization and thus increasing
both the density of depolarization current in demyelinated areas and thepossibility of reaching the threshold. Pharmacologic inhibition of Na+,K+ ATP has led to the increase of nervous impulse conduct in rats withspine demyelinated lesions. A possible explanation of this fact may bethat partial blocking of electrogenic pump will depolarize the axons andbring them in the proximity of threshold.
Key words: nervous conduct, demyelinated axons106. NOVELTIES IN ELETROPHYSIOLOGIC AND
BIOMECHANIC DATA ACQUISITION AT PATIENTSWITH MULTIPLE SCLEROSISMARIUS CRISTIAN NEAMŢU¹, ELENA TAINA AVRAMESCU2¹University of Medicine and Pharmacy Craiova, Faculty of Midwives
and Medical Assistance, Department of Normal and Pathologic
Physiology
2
University of Craiova, Faculty of physical Education and Sport,
Department of Human Anatomy
Priorities in analysing human movement are represented by dynamic
approach of the involved biosystems. In fields such as virtual reality
and computerized graphics the researchers are trying to use different
methods to stimulate human movements. The most practical method
is considered the use of a seizure system and movement analysis(Knudson and Morrison, 1997).Movement analysis is a non-invasive
technique and the quantitative determination of the dimensions by image
aquisition system does not influence the behaviour of the investigatedsubject. The research made prospective random studies regarding theefficiency of functional electrical stimulation on a number of 20 subjects
who followed a programme at the Center of Study and Research of Human
Movement, University of Craiova. The study used a distribution platform

33

Official Journal of the Romanian Society of Physiological Sciencesof plantar pressure Footscan Scientific Version, RSscan International,
Olen, Belgia. Studied parameters:image of pressure distribution on bothsoles for each movement moment;force value for each time unit, for theten specific zones; forţe: hallux, Phalanx, metacarpal bones, medianezone, medial and lateral zones of the heel; average pressure value
for the ten zones, depending on time; surface of the ten contact zones
for each frame; specific angles for each frame (depending on time);
speed of charging the surfaces, for each frame. The recordings madeby the use of pressure platform allowed the objective study of analysed
patients` walking defficiencies such as crural defficiency, bilateral femoral
defficiency, orthostatism and spastic walking, bilateral crural pyramidal
hypertonia, clonoidia at lower members.
Key words: pressure platform, movement analysis

107. CALCIUM IONS AND OSTEOCALCIN – MARKERSOF BONE ACTIVITY IN OSTEOPOROSISLILIANA NEO-LACULICEANU1, CAMELIA GURBAN1, FELICIASFRIJAN1, LAURA GOTIA2, SMARANDA GOTIA2, M. COJOCARU3,
V. ERDELEAN4, IASMINA SAVESCU5, CAMELIA CIACLI6, MARIAPUSCHITA6’’Victor Babes” University of Medicine and Pharmacy, Biochemistry
Department, Timisoara, Romania
2’’Victor Babes” University of Medicine and Pharmacy, Physiology
Department, Timisoara, Romania
3’’Titu Maiorescu’’ University of Medicine and Pharmacy, Physiology
Department, Bucharest, Romania
4
Municipal Clinical Hospital, Clinical Laboratory Department, Timisoara,
Romania5’’Banat’’ University of Agricultural Sciences and Veterinary Medicine,
TST-SHS Department, Timisoara, Romania
6’’Vasile Goldis” University of Medicine and Pharmacy, Immunology
Department, Arad, Romania
1

Background: Calcium ions and osteocalcin play a role in boneturnover at postmenopausal osteoporosis. Objectives: To analyze if serum
levels of osteocalcin and bone levels of calcium ions are elevated at
postmenopausal osteoporosis. Material and Methods: The study was madeon two cohorts of patients with postmenopausal osteoporosis (dependingon estrogenic deprivation period) comparing them with control group(postmenopausal women without osteoporosis). The serum levels of theosteocalcin were measured by enzyme-linked immunoasorbent assay
technique, and bone levels of the calcium ions were measured by thebone flame atomic absorption spectrometry analyzed. Results: In cohort
1 (below 15 yrs of estrogenic deprivation) serum levels of osteocalcin
were 20.12±0.87 ng/ml and bone levels of calcium ions were 10.65±0.03
mg/g of bone. In cohort 2 (over 15 yr of estrogenic deprivation) serum
levels of osteocalcin were 15.12±1.55 ng/ml and bone levels of calcium
ions were 11.63±0.14 mg/g of bone. In control group serum levels of
osteocalcin were 16.22±1.62 ng/ml and bone levels of calcium ionswere 14.24±0.13 mg/g of bone. Conclusions: The increased serum
levels of osteocalcin demonstrates osteoblasts activation (cohort 1), andthe decreased serum levels of osteocalcin demonstrates osteoblasts
apoptosis stimulation (cohort 2), associated with estrogens deficiency
postmenopausal installed. Calcium ions decreases at bone level (cohort
1 and 2) have as a consequence localized bone demineralization, being
favorable to bone microfractures appearance.
Key words: osteocalcin, bone calcium ions, bone turnover.

34

108. EVALUATION OF INTERLEUKIN-18 IN PATIENTSWITH MYOCARDIAL INFARCTION - BIOMARKER
OF ATHEROSCLEROTIC PLAQUE INSTABILITY
LILIANA NEO-LACULICEANU1, FELICIA SFRIJAN1, MADALINAGOLIEANU2, SMARANDA GOTIA3, MARIA PUSCHITA4, MANOLECOJOCARU5, CAMELIA CIACLI4, LAURA GOTIA3, CAMELIAGURBAN1
Biochemistry Department, “Victor Babes” University of Medicine andPharmacy, Timisoara, Romania
2
Individual Medical Center, Galati, Romania
3
Physiology Department, “Victor Babes” University of Medicine andPharmacy Timisoara, Romania
4
Cardiology Department, “Vasile Goldis” University of Medicine andPharmacy, Arad, Romania
5
Physiology Department, “Titu Maiorescu” University of Medicine andPharmacy, Bucharest, Romania

1

Objective: This study was conducted to analyze the circulating levelof interleukin-18 in patients with acute myocardial infarction, unstableangina, stable angina, and to estimate the proportion of immuneinflammatory answer prevalent feature in coronary artery disease.
Material and methods: Samples were obtained from patients withacute myocardial infarction (70), unstable angina (40), stable angina(50) and from control subjects (40). Enzyme-linked immunoasorbent
assay was used for quantification of serum levels of Interleukin-18.
Results: Compared with the control group (0.89±0.15 pg/ml), the levelsof Interleukin-18 were significantly higher in the all groups of patients(acute myocardial infarction: 5.87±1.20 pg/ml, p<0.05; unstable angina:
3.82±0.70 pg/ml, p<0.05; stable angina: 2.54±0.44 pg/ml, p<0.05).
There were no statistically significant differences in the concentrationof Interleukin-18 among the angina groups (p>0.05). Conclusions: Theconcentrations of Interleukin-18 were significantly higher in the coronary
artery disease groups. These results were related to the inflammatory
markers responses in atherosclerosis and suggest that Interleukin-18 is
involved both, in initial stages of development of coronary atherosclerosis
and in circumstance of plaque destabilization.
Key words: interleukin-18, atherosclerosis, coronary artery disease,and inflammation.

109. EXPRESSION OF ADHESION MOLECULESAFTER INCUBATION WITH PRO-INFLAMMATORYMEDIATORS OF ENDOTHELIAL-LIKE CELLSDERIVED FROM HUMAN MESENCHYMAL STEMCELLSDACIANA CARMEN NISTOR, IOANA SISKA, CARMEN BUNU,GABRIELA TANASIE, FLORINA BOJIN, DANA PLESCA, VIRGIL
PAUNESCU
University of Medicine and Pharmacy Victor Babes, Timisoara, Departmentof Phisiology and Immunology
Abstract : Purpose. 1. Isolation of human mesenchymal stem cells(MSCs) from bone marrow and differentiation toward the endothelial
lineage. 2. Morphologic characterization of endothelial-like cells (ECs)Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
obtained. 3. Behavior study of ECs obtained in culture using expression
analysis of adhesion molecules (ECAM) inducible on luminal ECs surface
after incubation with pro-inflammatory mediators.
Materials and methods. After bone marrow extraction from sternum,
posterior-superior iliac crest and femoral head, MSCs were isolatedusing positive and negative selection procedures. Cells were cultured inpresence of culture media supplemented with VEGF. ECs obtained werecharacterized using contrast phase microscopy, immunohistochemistry
staining for surface antigen CD31 and von Willebrandt factor, as well as
PCR analysis of KDR, vWF, VE-cadherin and eNOS expression. Further
behavior studies of ECs in culture conditions were performed considering
analysis of ECAM, CD54, CD106, CD62E and CD62P expression, under
the circumstances of incubating HUVEC and ECs cultures in presenceof TNFalfa, LPS and H2O2 added in similar concentrations to thosegenerated in vivo and/or in vitro in pathologic conditions. Results. Bonemarrow-derived MSCs isolated by adherence methods, cultivated inappropriate conditions can give rise to ECs after 7-14 days of culture.
CD54 and CD106 expression was not changed after stimulation with
TNFalfa, LPS, H2O2, similar to the results obtained using pre-incubation
with TNfalfa or LPS, before short-time incubation with H2O2, whichsuggested that these cells are functionally immature. However, vonWillebrandt factor, CD31, eNOS, KDR and VE-cadherin expression in
ECs indicated that these cells exhibit the characteristics of endothelial
progenitors and thus can be used for therapeutic purpose.
Keywords: mesenchymal stem cells, endothelial cells, immaturefunctionally, adhesion molecules.

110. ALDOSTERONE RAPIDLY POTENTIATESBOTH THE NO AND EDHF COMPONENTS OF
ENDOTHELIUM-DEPENDENT RELAXATION IN
RAT MESENTERIC ARTERIESOPRISA C, SERBAN IL, MUNGIU OC, BRANISTEANU DD,SERBAN DN“Grigore T. Popa” University of Medicine and Pharmacy, Iasi,
Romania
Despite variable vascular effects, the rapid nongenomic action ofaldosterone generally attenuates the effect of vasoconstrictor agents,
due to nitric-oxide-synthase activation (via mineralocorticoid receptors
and the HSP90/PI3K/PKB pathway), but also potentiates vasoconstriction
in absence of endothelium. We investigated for the first time the effectof aldosterone (1 nM to 1 microM) upon the EDHF response, using
isometric myography of rings (1 mm wide) from mesenteric artery andfirst order branches, obtained from male Wistar rats (200-250 g). After
1 h equilibration under a tension of 1 g the vessels were checked forabsence of myogenic response to stretch and for complete endotheliumdependent relaxation (EDR) induced by carbachol (0.01 mM) whenprecontracted by 0.01 mM phenylephrine. We tested the EDR inducedby carbachol (100 nM to 0.1 mM) in phenylephrine-precontracted
rings, as global effect and its EDHF component (in presence of 0.1
mM L-NAME and 0.01 mM indomethacin). Results were expressed as
residual active tension (% of precontraction level; mean ± SEM; n6).
We observed that aldosterone induces contraction in the presenceof L-NAME. It NOS-dependently inhibits the contractions induced byphenylephrine (10 nM to 10 microM) or high extracellular K (30 to 90mM), but far from the pronounced effects in renal arterioles which result
from NOS activation by aldosterone. Moreover, aldosterone potentiates

Fiziologia ‑ Physiology  2009 Supplement

both the global EDR and its EDHF component, but these effects arealso relatively weak and they are visible only for concentrations above
10 nM aldosterone (which is beyond the physiological range). Supported
by Romanian Grant CNCSIS-A1222/2007-2008.
Key words: aldosterone, endothelium, EDHF, resistance arteries

111. ANTIEPILEPTIC EFFECTS OF SOMATOSTATINEIN LOW MAGNESIUM INDUCED SEIZURE LIKEEVENTSORBÁN-KIS KÁROLY1,2, ANTAL KÁROLY2, SZILÁGYI TIBOR1,
KARDOS JULIANNA2, EMRI ZSUZSA2Department of Physiology, University of Medicine and Pharmacy, Targu
Mureş Romania
2
Department of Neurochemistry, Chemical Research Center, Hungarian
Academy of Sciences, Budapest, Hungary

1Somatostatine, a widely distributed neuropeptide in the brain andespecially in the hippocampus, is associated with seizures both inanimal models and human temporal lobe epilepsy. It has been shownthat somatostatine containing neurons are sensitive to seizure induced
death and it is hypothesized that the loss of somatostatine function in the
hippocampus could contribute to epileptogenesis. This effect appears to
be related in rats and humans to somatostatine receptor subtype 2.We
used the in vitro low magnesium model to induce seizure like events in
sections from juvenile rat hippocampus. We recorded from area CA3of the rat hippocampus extracellular field potentials during interictal,
preictal and ictal periods of seizures. Than we applied somatostatine inorder to observe its effect on seizure like activity and interictal spiking.
Somatostatine decreased the length of low magnesium induced seizures
by 18.4±12.9% and increased the interval between them by 14.1±17.2%
when compared to control. This effect was reversible by washout. We
also studied interictal spiking patterns during seizure like events withand without somatostatine. Because the somatostatine neurons areclearly compromised in post-seizure hippocampus and somatostatine
appears to have an important antiepileptic effect one can rationalize
for the utilization of this drug or its derivates for treating temporal lobe
epilepsy. However several caveats are to be considered such as receptor
desensitization or disruption of the effect of endogenous somatostatineon the central nervous system.

112. THE ULTRASOUND EVALUATION OF THE
OSTEOPOROSIS RISK IN YOUNG ADULTS WITH
AND WITHOUT MENTHAL DISABILITIESRALUCA PAPACOCEA1, ANCA BADARAU1, T.PAPACOCEA2,
CATALINA CIORNEI1, MARIANA ARTINO1, MAGDA BURAGA3,IOANA STEFANESCU3
Physiology Dept. I Carol Davila University of Medicine andPharmacy
2
Sf. Pantelimon Emergency Hospital Bucharest
3
Physiology Dept. II Carol Davila University of Medicine andPharmacy1

Osteoporosis occurs as a consequence of loss of bone mass, usually35

Official Journal of the Romanian Society of Physiological Sciences
attributed to age, and the decreased quality of the micro-architectureof bone, which results in a bone which is more susceptible to fracture
and its incidence continuously increasing. The study objective was toelucidate if young sedentary adults and individuals with menthal disabilities- Down’s syndrome - (DS) experience a different risk of osteoporosis.
The bone mineral density was measured in 30 sedentary young adults
and 42 young adults with menthal disabilitation, versus 22 control
subjects of both genders. Bone densitometry results were determinedusing the Sahara Clinical Bone sonometer and were reported in termsof quantitative scores that compare this result to reference ranges forpeers of the same sex and ethnicity. We used the T-score which isdefined as the difference between the individual patient’s results and themean results obtained in a young adult population, expressed in units of
the young adult population standard deviation. The Sahara equipment
measured the speed of sound (SOS, in m/sec) and the broadband
ultrasonica (BUA, in dB/MHz) of an ultrasound beam passed throughthe heel. The SOS and BUA results are combined linearly to obtain thequantitative. Ultrasound Index (QUI), the same type of result reported by
x-ray based bone densitometry systems. The obtained results showeda slightly decreased bone density in both young sedentary adults withor without menthal disabilities versus control, but only the group withmenthal disabilities showed a significant reduction (p0,019).
Key words: osteoporosis, ultrasound, bone density

113. THE EFFECT OF ALLIUM OBLIQUUM ON NITRICOXIDE SYNTHESIS AND OXIDATIVE STRESS IN
ACUTE EXPERIMENTAL INFLAMMATIONPARVU ALINA ELENA, PARVU MARCEL, VLASE LAURIAN“Iuliu Haţieganu” University of Medicine and Pharmacy
Allium obliquum is a very rare perennial which is encounteredin Romania in a single place, on limestone rocks in Turda Gorges. It
has been shown that Allium species have high concentration of total
flavonoids, high content of carotenoids and chlorophylls. Therefore in thisstudy we investigated the effects of A. obliquum extract on nitric oxide
synthesis and oxidative status in an acute experimental inflammation.
We used 6 groups of Wistar-Bratislawa male rats with turpentine oilinduced inflammation: a positive inflammation control with no treatment,
three groups treated with diffrent A. obliquum extract concentrations, agroup treated with with L-NAME, and a group treated with trolox. NO
synthesis was evaluated by serum nitrites and nitrates measurement,
and oxidative stress was evaluated by total oxidative status and total
antioxidative reactivity determination. The results showed that: Allium
obliquum reduce nitric oxide synthesis and oxidative stress in a dose
dependent way; the inhibitory effect of A. obliquum on NO synthesis
was smaller than that of L-NAME; the antioxidative effect was smallerthat that of trolox. In conclusion, in acute experimental inflammation A.
obliquum extract had an antiinflammatory effect due to the inhibitory
action on NO synthesis and oxidative stress.

114.STRUCTURE AND ACTIONS OF DOTHELINSCRISTINA PETRICA1, DANIELA BADEA2, M. BADEA3, ANDREEAPETRICA4
Department of Pathophysiology, University of Medicine and Pharmacy

136

of Craiova
Department of Physiology, University of Medicine and Pharmacy of
Craiova3
Department of Hematology, University of Medicine and Pharmacy of
Craiova
4
Resident doctor, University of Medicine and Pharmacy of Craiova
2

Three isoforms of endothelins have been identified: ET-1, ET-2 andET-3. Each isoform is a product of a different gene and is synthesized asa prepro form that is processed to a propeptide and then to the mature
peptide. Each endothelin is a 21-amino-acid peptide containing two
disulfide bridges. Endothelins are widely distributed in the body. ET-1
is a predominant endothelin secreted by the vascular endothelium.
It is also produced by neurons and astrocytes in the central nervous
system and in endometrial, renal mesangial, Sertoli and other cells.
ET-2 is produced predominantly in the kidneys and intestine, whereas
ET-3 is found in highest concentration in the brain but is also present
in the gastrointestinal tract, lungs, and kidneys. The expression of theET-1 gene is increased by growth factors and cytokines, includingtransforming growth factor-β (TGF-β) and interleukin-1 (IL-1), vasoactive
substances including angiotensin II and vasopressin and mechanicalstress. Expression is inhibited by nitric oxid, prostacyclin and atrial
natriuretic peptide. Endothelins exert widespread actions in the body.
They cause dose-dependent vasoconstriction in most vascular beds.
Intravenous administration of ET-1 causes a rapid and transient decreasein arterial blood pressure followed by a prolonged increase. Endothelins
are potent coronary vasoconstrictors. They act on the kidney to cause
vasoconstriction and decrease glomerular filtration rate and sodium andwater excretion. In the respiratory system, they cause potent contractionof tracheal and bronchial smooth muscle. They exert a variety of actions
on the central and peripheral nervous system, the gastrointestinal system,
the liver, the urinary tract, the skin.

115. THE METABOLISM OF ARACHIDONIC ACID
BY 5-LIPOXYGENASE
CRISTINA PETRICA1, DANIELA BADEA2, M. BADEA3, ANDREEAPETRICA4
Department of Pathophysiology, University of Medicine and Pharmacy
of Craiova
2
Department of Physiology, University of Medicine and Pharmacy of
Craiova3
Department of Hematology, University of Medicine and Pharmacy of
Craiova
4
Resident doctor, University of Medicine and Pharmacy of Craiova

1

The metabolism of arachidonic acid by the 5-, 12-, and 15 lipoxigenases(LOX) results in the production of hydroperoxyeicosatetraenoic acid
(HPETEs), which rapidly convert to hydroxy derivatives (HETEs) andleukotrienes. The most actively investigated leukotriens are those producedby the 5-lipoxigenase present in inflammatory cells (polymorphonuclear
leukocytes-PMN, basophils, mast cells, eosinophils, macrophages).
Stimulation of these cells elevates intracellular Ca 2+ and releases
arachidonate; incorporation of molecular oxygen by 5-LOX, in association
with 5-LOX-activating protein (FLAP), then yields the unstable epoxide
leukotriene A4 (LTA4). This intermediate either converts to the dihydroxy
leukotriene B4 (LTB4) or conjugates with glutathione to yield leukotriene
C4 (LTC4). LTC4 and LTD4 are potent bronchoconstrictors and arerecognized as the primary components of the slow reacting substance

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciencesof anaphylaxis (SRA-A) that is secreted in asthma and anaphylaxis.
LTA4, the primary product of 5-LOX, can be converted via 12-LOX in
platelets to the lipoxins LXA4 and LXB4. Lipoxins have diverse effects
on leukocytes, including activation of monocytes and macrophages
and inhibition of neutrophil, eosinophil, and lymphocyte activation.
Both lipoxin A and lipoxin B inhibit natural killer cell cytotoxicity. The
actions of lipoxygenase generate compounds that can regulate specificcellular responses important in inflammation and immunity. Leukotriene
B4 (LTB4) is a potent chemoattractant for PMNs, eosinophils andmonocytes; LTC4 and LTD4 are potent chemoattractant for eosinophils.
At higher concentrations, these leukotrienes also promote eosinophil
adherence, degranulation and oxygen radical formation. The leukotrienes
have been implicated in the pathogenesis of inflammation, especiallyin chronic diseases such as asthma and inflammatory bowel disease.
PGE2 inhibits the differentiation of B lymphocyte into antibody-secreting
plasma cells and depress the humoral antibody response. It also inhibits
mitogen-stimulated proliferation of T lymphocytes. PGE2 and TXA2 may
also play a role in T-lymphocyte development by regulating apoptosisof immature tymocytes.

116. THE SALIVARY OXIDATIV BALANCE INPERSONS WITH INCORRECT DENTAL CORONARY
RECONSTRUCTION

had also an obvious increase of the MDA(p<0,2). In cases of persons
with incorrect obturations with composite materials the ceruloplasmine
had decreased values compared to those of the control group. (p<0,001).
Compared to the control group, the ceruloplasmine of the persons
with incorrect dental coronary reconstruction was also significantly
low (p<0,001). It has been measured also the uric acid from the saliva,
which fulfills an antioxidant role. In cases of persons with improper
obturations with composite materials and those with incorrect coronary
reconstruction the values of the uric acid was situated between the limitsof the control group (p>0,1). The values of the alkaline phosphatase incases of persons with incorrect obturations with composite materials
was decreased insignificanly (p>0,1). In cases of persons with improper
dental coronary reconstruction there was observed the same variations(p>0,1). Persons with incorrect obturations using composite materials had
a decreased enzymatic activity of the acid phosphatase as comparedto the control group (p<0,5). The values of the acid phosphatase incases of persons with improper coronary reconstruction have beenslightly reduced (p>0,1). Conclussions.Persons with obturations using
composite materials and incorrect dental coronary reconstruction wereassociated with an increase salivary of ROS.The presence of vascularlessions in the electromicroscopical field, of leucocytes, macrophages,
mastocytes, desmosoms, fibroblasts and altered collagen fybres
represent microscopic signs of the inflamation. Key words: coronary
reconstruction, oxidative stress, ceruloplasmine, saliva.

ADRIANA PIRTE1, CAMELIA DALAI1, CIPRIAN DALAI1, MARIANA
MURESAN1, OTILIA MICLE1, LIANA MICLE1, ALINA VENTER1,
LUCIANA DOBJANSCHI 1, ANGELA ANTONESCU1, MIRCEADOROFTEIU2

117. NEUROBIOLOGY OF POST-ISCHEMIC
RECUPERATION IN THE AGED MAMMALIAN
BRAIN

Medicine and Pharmacy Faculty Oradea, Romania
2
University of Medicine and Pharmacy Faculty, Cluj Napoca

AUREL POPA-WAGNER1, CHRISTINE POEHLKE1, JOSEFINE
KAISER1, ANA-MARIA BUGA2

1

Introduction. We have intended to prove the existance of a concordance
between the level of the salivary ROS and electromicroscopical changes
in the marginal periodont, damaged by inappropriate stomatologicalinterventions. Material and method. There have been studied 65 persons;students in dental medecine at Medicine and Pharmacy Faculty of
Oradea. They are all aged between 20–30 years, non-smokers. These
were divided in 25 with improper obturations with composite materials
and 25 had inappropriate coronary dental reconstruction. At the sametime, a group of 15 persons with no restauration has been studied. The
oxidativ stress markers utilized were MDA and ceruloplasmin. It also wasmesured the concentration of uric acid with enzymatic-colorimetric uricase
PAP using reactive Greiner Diagnostic, Germany, on analyzer HITACHI
912. Together with the testing of the oxidative stress for the two groupsof subjects ,it have been determined acid and alkaline phosphatase. The
total acid phosphatase it has been assesed with the colorimetric method
using reactive Randox Laboratories Ltd, Great Britain, cat No AC 1011
on analyzer HITACHI 912, Roche Diagnostic, Switzerland. The alkaline
phosphatase was evaluated by the Kinetic optical clorimetric method
DGKC, using reactive Greiner Diagnostic, Germany, cat No 105203, on
analyzer HITACHI 912, Roche Diagnostic, Switzerland. The prelevationsof the pathological and normal tissues have been made from persons
aged between 20 and 30 years and studied at the Transmission Electonic
Microscope (TEM), type Jeol JEM 1010 from The Centre of Electronic
Microscopy of Babes Bolyai University in Cluj-Napoca, Romania. Results.
The research made in cases of persons with incorrect obturations withcomposite materials have shown an insignificant increase of the MDA(p<0,2). The persons with inappropriate dental coronary reconstruction

Fiziologia ‑ Physiology  2009 Supplement

Klinik für Neurologie, Ernst-Moritz-Arndt-University, Greifswald,
Germany.
2
University of Medicine and Pharmacy, Craiova, Romania.

1

Old age is associated with an enhanced susceptibility to stroke andpoor recovery from brain injury, but the cellular processes underlying
these phenomena are uncertain. Therefore studying the basic mechanism
underlying functional recovery after brain ischemia in aged subjected it
is of considerable clinical interest.
Potential mechanisms include neuroinflammation, changes in brain
plasticity-promoting factors, unregulated expression of neurotoxic factors,
or differences in the generation of scar tissue that impedes the formationof new axons and blood vessels in the infarcted region. Available data
indicate that behaviorally, aged rats were more severely impaired byischemia than were young rats, and they also showed diminishedfunctional recovery. Further, as compared to young rats, aged rats
develop a larger infarct area, as well as a necrotic zone characterized bya higher rate of cellular degeneration, and a larger number of apoptotic
cells. Both in old and young rats, the early intense proliferative activity
following stroke leads to a precipitous formation of growth-inhibitingscar tissue, a phenomemon amplified by the persistent expression ofneurotoxic factors. Finally, the regenerative potential of the rat brain islargely preserved up to 20 months of age but gene expression temporally
displaced, has a lower amplitude, and is sometimes of relatively shortduration. Most interestingly it has recently been shown that the humanbrain can respond to stroke with increased progenitor proliferation inaged patients opening the possibilities to utilize this intrinsic attempt for37

Official Journal of the Romanian Society of Physiological Sciences
neuroregeneration of the human brain as a potential therapy for stroke.
Given the heterogeneity of stroke, a universal anti-inflammatory solution
may be a distant prospect, but probably neuroprotective drug cocktails
targeting inflammatory pathways in combination with thrombolysis may
be a possibility for acute stroke treatment in the future.

118. CONNEXIONS BETWEEN GLYCOSILATEDHEMOGLOBIN AND ALBUMINURIA AT A CHIELD
WITH DIABETESLAURA POPESCU1, BUBUIANU ANCA1, MIHU MIHAELA2
UMF ”Carol Davila” Bucuresti Departament of Physiology II
Diabetes and Nutritional Diseases Clinic of Emergency Hospital forChildren “M.S. Curie”

1

2

The objectiv of the study is to outline kidny micorangiopaty inchildren, in relation with the variation of the glycosilated hemoglobin.
The study was conducted in the Diabetes and Nutritional Diseases Clinicof Emergency Hospital for Children “M.S. Curie”, with the supermisionof Dr. Mihu Mihaela. The study was made on a lot of 129 children,
durind 3 years, whitch we studied the correlation between the levels of
glycosilated hemoglobin and the begining of the onset of complications
due to kidney microangiopathy trough microalbuminuria. The results
showed that in 1/3 of the children that presented warying levels of over
7% of glycosilated hemoglobin, microalbuminuria was installed at aperiod of an aproximately 2 years, so at 3 years from the onset of theillness, the kidney microangiopaty was alredy installed. Final concolusion:
complication can be prevented by maintaining a glycemic balance, andto do so there must be a permanent cooperation between the physician,the familiy and the pacient, in our case with the child.
Key wors: diabetes, glycosilated hemoglobin

119. THE METABOLIC RISK IN PATIENTSWITH SUBCLINICAL AND CLINICALHYPOTHYROIDISM
PROTOPOP SVETLANA1, LYSYI LEONID1, ZOTA LARISA2,BOBCOVA SVETLANA1, GANENCO ANDREI3
Department of Biochemistry and Clinical Biochemistry, State Universityof Medicine and Pharmacy “Nicolae Testemiţanu”, Chişinău, Moldova
2
Department of Human Physiology, State University of Medicine andPharmacy “Nicolae Testemiţanu”, Chişinău, Moldova

1

Objective: The aim of this study was to explore the hypothesis that
subclinical and clinical hypothyroidism is associated with componentsof the metabolic syndrome. Design and methods: Twenty women with
subclinical hypothyroidism (elevated TSH and normal thyroxine levels)and nineteen women with clinical hypothyroidism (elevated TSH anddiminished thyroxine levels) were compared to twenty-seven female
controls matched for age. None of the patients had been previously
treated with thyroxine. In all participants we determined thyroid
stimulating hormone (TSH), free triiodthyronine and free thyroxine levels,
lipid and lipoprotein concentrations, fasting glucose and insulin levels,
homeostasis model assessment of insulin resistance (HOMA-IR), we
measured waist circumference, systolic and diastolic blood pressure.
The metabolic syndrome was defined according to the International

38

Diabetes Federation criteria. Results: The patients with subclinicaland clinical hypothyroidism are at greater risk of metabolic syndrome
than the healthy subjects (relative risk, 2,43; 95% confidence interval,
0,99-5,95, and relative risk, 1,99; 95% confidence interval, 0,75-5,29,
respectively). In the hypothyroid patients insulin positively correlated with
TSH (r0,33, p<0,05) and negatively correlated with free triiodthyronine(r-0,42, p<0,01). The thyroid function tests were associated only withhigh-density lipoprotein cholesterol levels (r0,34, p<0,05 for TSH, r-0,32,
p<0,05 for free triiodthyronine and r-0,49, p<0,01 for free thyroxine). The
HOMA-IR positively correlated with waist circumference (r0,68, p<0,001),
triglyceride levels (r0,61, p<0,001), systolic blood pressure (r0,48, p<0,01)
and diastolic blood pressure (r0,38, p<0,05).Conclusion: Subclinicaland clinical hypothyroidism is associated with insulin resistance thatmight contribute to the development of the metabolic syndrome foundin these conditions.
Key words: hypothyroidism, metabolic syndrome, insulin
resistance120. IMPORTANCE OF NERVOUS AND
ENDOCRINE FACTORS IN DETERMINATIONOF SOME ELECTROCARDIOGRAPHIC
MODIFICATIONS IN WOMEN AFTER 50 YEARS
G. RACA, OANA MARIA RICA
University of Medicine and Pharmacy of Craiova
Statistical and clinical studies (The Framingham Study, American
Heart Association–AHA) show an increase risk for cardiovasculardiseases, especially Coronary Heart Diseases (CHD) and Hypertension
in menopausal women, after 50 years. It is accepted that decreaseof oestrogens secretion and loss of their protection is a main factor
which cause this phenomenon. Thus menopause is considered amajor risk factor for CHD. Electrocardiogram (ECG) in these womenshows nonspecific modifications, such as modification of repolarization
phase, extrasystoles, tachycardia or bradycardia, branch bundle
block, left ventricular hypertrophy. What is signification of these ECG
modifications, these are functional modification or CHD manifestations?
Electrocardiogram was recorded in women with ages 40-65 years, inrest condition and after betablocants administration. These women had
anterior good health, without cardiovascular diseases in their life, but in
moment examination in some postmenopausal women was presented
some risk factors such as increase vegetative tonus, various stressconditions, hypercholesterolemia, diabetes, hypertension, smoking.
Results show that 40,5 % of postmenopausal women had various andnonspecific ECG modifications, such as modifications of repolarization
phase, sinusal tachycardia >100 beats/ minute, sinusal bradycardia
50-60 beats/ minute, atrial or ventricular extrasystoles isolated or 2-4
grouped, low amplitude or absence P-waves, elongation of Q–T interval,
first degree atrioventricular block, left or right minor branch bundle
block, left ventricular hypertrophy. These modifications were remarked
in two or more leads with aleatory distribution, without certain coronary
localization. Modifications of repolarization phase were detected in
30,2 % of women and consisted in depression of ST- segment ≥1mm
(horizontal, downsloping or upsloping), plate, ample, peaked T- waves,
inversion of T- waves, elevation of U- waves. In some women was
associated two or three modifications, more frequently ST–T modifications
with extrasystoles and tachycardia. Administration of betablocants inwomen with modifications of repolarization phase and extrasystoles are

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
redressed ECG route in 53,4 % and no changed ECG route in 46,6 %.
In 45% of these women, there are some clinical manifestations, suchas pain in heart area, sometimes anginous pain, palpitation, tachycardia
or bradycardia, hypertension etc., existed a positive correlation with
ECG modifications. In conclusion, in postmenopausal women appear
nonspecific ECG modifications especially in repolarization phase.
These modifications may be caused by increase vegetative tonus andby electrolytes disturbances, but may show a coronary ischaemia whenis associated with other ECG modifications (extrsystoles, tachycardiaetc.) and with clinical manifestations such as heart pain, various risk
factors etc.

121. THE EFFECT OF NOISE ON URINARYCATECHOLAMINES FOR MARITIMENAVIGATING PERSONNEL
NINELA RADULESCU, ILEANA ION, CEAMITRU N., CECILIAADUMITRESI, CRISTINA FARCAS, CARMEN CIUFU, LOREDANAHANZU-PAZARA
Physiology and Pathophysiology Department, Faculty of Medicine,“Ovidius” University, Constanta
The aim of the study is to establish whether the exposure to loudnoise (greater than 87 dB) onboard naval ships is able to modify
urinary catecholamins excretion. The psychological characteristics of
personality of the subjects play an important role in the stress response.
The noxious effect of noise level have been studied on two groups ofsubjects: engine crew and deck crew, taking into account the age, thelength of sea service, periodicity of embarkments and the psyhical andneuropsychic stress. Samples were collected before leaving and after
returning from the sea voyage. One further urine sample was collected
after two monthes of rest . Our study shows that exposure to noise
induces significant increases of urinary catecholamines (p<0,001). The
analysis of data showed statistically significant differences between theurinary catecholamines values after noise exposure, higher among theengine crew than among the deck crew.
Key words: noise, catechomanines, maritime navigating
personnel

122. The Synthesis of blood glucose
control system by using artificial neuralnetworks
IULIA ALEXANDRA RISTEA, LILIANA IONESCU, IONELAIANCUUniversity of Medicine and Pharmacy of Craiova, Faculty of Medicine
We consider the neural networks an attractive solution for themodeling of physiological control systems modeling and simulation.
Neural networks are capable of discovering hidden dependency
starting only from the available database, without imposing modeling
restrictions. After a successful learning period, neural networks tolerate
in a remarkable way the differences (sometimes appreciable) between
the data applied at the entering of the operating process and the ones
seen in the training phase. This is a consequence of the so-called
generalization capacity of neural networks. This capacity expresses

Fiziologia ‑ Physiology  2009 Supplement

the neural network’s capability to offer a correct response, even if at
the entry versions are incomplete, noisy or distorted information fromthe training phase. Using experimental data resulted after laboratory
analysis, the authors have realized feed-forward neural networks for themodeling of the glycaemia control system. After the learning process,the network is capable of providing the insulin concentration in the
blood (when glycaemia is known) and anticipate the glycaemia values
when the amount of insulin taken in is known. We mention, that even
if the network sustains its structure, it must be trained in particular for
every individual.
Key words: blood glucose control, mathematical model, artificial
neural network

123. EVALUATION OF PATIENTS WITH ADVANCEDSTAGE CHRONIC OBSTRUCTIVE PULMONARYDISEASECODRUŢ ROMAN1, VOICU TUDORACHE2, GEORGETAMIHALAŞ1, LEONARD MADA3, TEODORA ROMAN2, CRISTIANOANCEA2
1
Department of Physiology, University of Medicine „Victor Babeş”Timişoara
2
Department of Lung Diseases, University of Medicine „Victor Babeş”Timişoara
3
Department of Medical Informatics, University of Medicine „VictorBabeş” Timişoara

Introduction: Chronic Obstructive Pulmonary Disease(COPD) is themost frequent lung illness, with increasing morbidity. It has a delayed
clinical simptomatology, which correlates often with advanced stagesof the disease. Materials and methods: We have investigated 100 incare patients, 41 with stage III COPD and 59 with stage IV, of which
11 women and 89 men. The average age was 61 years. Average FEV1
value was 37,84% for stage III disease and 25,42% for stage IV disease.
We evaluated the patients by forced spirometry, maximal inspiratory
pressure(MIP), maximal expiratory pressure(MEP), nutritional staus
and oxigen saturation(SaO2). We focused on these methods because
they are easy to be determined and are accurate for the evaluation ofpatients status. Results: Decreases in MIP and MEP were correlatedwith FEV1 and FVC, sex and muscle mass. SaO2 was decreased onlyin stage IV (average 87%). Conclusions: Worsening of simptomatology
and decreased physical capacity favor sedentary state and anxiety, whichlead to physical and mental deconditioning with decrease in quality oflife We wanted an efficient evaluation of patients with advanced COPD
in order to obtain a better disease management through medication,oxigen therapy and pulmonary rehabilitation.

124. CROSS-CORRELATION BETWEEN TWO
SURFACE EMG SIGNALS FROM THE SAMEMUSCLE IN MAXIMAL CONTRACTION
F. ROMANESCU1, A. NEŞTIANU2, P. BADEA3, G. POPESCU4,
V. NEŞTIANU1
1. Physiology, University of Medicine and Pharmacy Craiova
2. Physiopathology, University of Medicine and Pharmacy Craiova

39Official Journal of the Romanian Society of Physiological Sciences
3. Biostatistics, University of Medicine and Pharmacy Craiova
4. Informatics Bureau, University of Medicine and Pharmacy Craiova
The purpose of the study was to evaluate the cross-correlation
between two channels of surface EMG (SEMG) on the same muscle
in activity. The study was conducted on a lot of 16 male subjects which
performed sustained isometric maximal contraction of the hand fingers
flexors. SEMG was recorded on two channels, the electrodes being placedon two parallel zones above the same muscle. The cross-correlation
analysis was performed between simultaneously recorded samples oneach channel of the SEMG filtered signal on successive periods of 500
ms, resulting a correlation coefficient (R) on each 500 ms of contraction.
We applied a linear regression equation on all R values from 500 to
500 ms., to evaluate the evolution of the correlation between the twochannels. The slope of regression line was slightly increasing (slope =0.0000948) during maximal voluntary contraction. These results indicatethat cross-correlation of two surface EMG channels recording from thesame muscle is increasing during maximal voluntary contraction, a factthat can be partially explained by increasing of the synchronization
between motor units.
Keywords: electromyography, muscle fatigue, isometriccontraction.

125. The influence of long-term hemodyalisis
on non-specific and specific body defense
mechanisms
Alexandra Floriana Rosu1, Lucica Rosu1, O. Zlatian1,
Cristiana Vaduva2, Daniela Badea3
UMF Craiova – Department of Microbiology
Emercency Hospital of Craiova – Department of Hemodyalisis
3
UMF Craiova – Department of Physiology

12Introduction. Infections are the second cause of death in patients
undergoing long-term hemodyalisis, after cardiovascular diseases. The
hemodyalisis procedure, due to the biological incompatible membranes
and impurities in dyalisis fluid accomplished by elevated seric levels ofurea, performs an aggression against non-specific and specific bodydefense mechanisms. Material and method. We studied a lot composedof 80 patients with chronic renal failure included in hemodyalisis program,in witch it was evaluated the renal function, hepatic metabolism, stateof nutrition correlated with hematological indices, acute inflammation
markers (fibrinogen, C reactive protein, erythrocyte sedimentationrate) and serum complement dosing. The blood tests establishedhemoglobin levels and percents of the leucocytes. We established thepercent of T total and helper lymphocytes by rosette tests. The results
obtained showed alterations of renal function (increased levels of urea,
creatinine and uric acid), modifications of non-specific and specificdefense mechanisms (activation of serum complement, increasingof acute inflammation markers, anemia, neutropenia, monocytosis,
lymphopenia by decreasing T total and helper lymphocytes). In several
cases the liver function was also altered. Conclusions. Modificationsof the investigated humoral and cellular parameters are correlatedwith hemodyalisis factors. In order to reduce the negative effects ofhemodyalisis procedure on non-specific and specific body defense
mechanisms it is advisable using a bio-compatible dialysis membraneand high purity dialysis fluid.
Key words: hemodyalisis, non-specific and specific body defense
mechanisms, renal function.

40

126. INFLUENZA VIRUSES INFLUENCE ON HUMANLYMFOCYTESALEXANDRA FLORIANA ROŞU, LUCICA ROŞU, O. ZLATIAN,DANIELA BADEA, T. DĂIANU, MARIA CRISTINA BEZNĂ
UMF Craiova, Department of Microbiology
Introduction. Because influenza viruses can infect the immune cells,
therefore diminishing the host ability to generate immune responses, it isnecessary an understanding of interrelations between the components ofimmune system, in order to apply the immune prophylaxis on scientificbases. Material şi metode. We investigated the humoral immune
response to the infection with circulating influenza viruses, on a lot
composed of 250 children aged between 1-15 years, hospitalized for
acute infections of upper and lower respiratory tract, in pediatrics clinic ofClinical Emergency Hospital of Craiova, between 1.10.2006-1.10.2008.
In order to confirm the influenza infection we used the hemagglutination
inhibition for testing the presence in the blood of specific antibodies
against influenza A or B viruses which circulated in the studied period.
The cellular immunity was investigated at 50 children with more severe
symptoms selected from this lot. We used the methods: normal andhigh affinity E rosette formation, blastic transformation of lymphocytes
test and macrophage inhibition test. The results obtained shows theeffects of influenza viruses influence on human lymphocytes (bothnumerically and functionally), on the humoral as well as on the cellularimmune response against antigenic variant of infecting influenza virus.
Conclusions: vaccination against influenza viruses is the specific methodof immune prophylaxis of influenza infections.
Keywords: influenza, cellular immunity, immune depression,
lymphocyte.

127. The INFLUENCE OF body composition OVER
FOOTBALL PLAYERS EFFORT CAPACITY
LIGIA RUSU1,2, DENISA ENESCU BIERU1, MIRELA LUCIA CĂLINA1,2,
ELENA TAINA AVRAMESCU1,2, FLORIN ROMANESCU3Faculty of Physical Education and Sport Craiova, University of Craiova,
Romania2
Sport Polyclinic Craiova, Romania
3
University of Medicine and Pharmacy Craiova - Department of
Physiology, Romania

1

Introduction
The body composition is an important parameter that evaluates
sportsmen´ physical training level and establishes their capacity
performance.
Objective
The aim of our study was to compare on the one hand the body fat
and bone mineral density during pre-season (first period) and after theoff season break (second period) at professional football players andon the other hand the values that had been obtained to the ones of
non-athletic young males, thus, to create a profile of physical featuresin professional football players.
Material and method
The study was made on twenty two male athletes with an averageage of 20 years from a professional football school which underwent for aperiod of one year, the determination of body fat mass and bone mineral

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
density (BMD) during pre-season and after the off season breaks.
We used the skin fold thickness over five sites to measure the body fat
mass and DEXA (dual energy X-ray absorption) to assess the BMD.
Results
The skin fold thickness measurements revealed that body fat mass
was higher immediately following the off season break (11,4%) thanpre-season (10,2 %).
Bone mineral density average value was higher (1,38g/cm2) in thefirst period than in the second one (1,33g/cm2).
The BMD average in football players was well above non-athletic
young males´ average (1.401g/cm2 compared to 1.23g/cm2), while thebody fat mass at the two groups was not significantly changed.
Conclusions
Physical activity influences positively the body composition, implicitly
the effort capacity.
Key words: football players, body composition, performance.

128. AGE INDUCED AMPLIFICATION OF THE
ALLOSTATIC LOAD DETERMINING SYNAPSE
INHIBITION?
A.SAULEA, V.MINCIUNA, V.KOBETS, B.MELNIC,
S.FABRIKANTOVA
Republican Center of Medico-Social Rehabilitation, Republic ofMoldova
Aim: Estimation of the central nervous structures motor activity
during age induced amplification of the allostatic load.
Materials and methods. 45 old persons (69.8±1.21 years) were enrolled
in study. The nervous system motor activity was estimated analyzing thephysiological tremor of the distal hand phalanx. The spectral energy oftremor, mean frequency of oscillations and their density in range of: 0-3.0
Hz; 4.0-6.9 Hz; 7.0-12.9 Hz; 13.0-18.9 Hz and 19.0-50.0 Hz have been
assayed. Results. In rest physiologic tremor spectral energy of tremor
was 310.7±623.28 ms2, mean frequency of oscillations – 8.2±0.34 Hz
and density -24.8±1.67 in 0-50 Hz. The mechanic reflex activation led
to an increase of spectral energy of tremor by l.6fold due to density
elevation by 4.6fold in high frequencies (19.0-50.0 Hz). The density
modulated mainly by central oscillator and spinal alpha-gamma loop
decreased: by 2.6fold in range 4.0-6.9 Hz and by l.8fold in 7.0-12.9 Hz.
Nonmodification of indices in range of 13.0-18.9 Hz may underline that
this frequency is simultaneously modulated by neuronal and mechanic
hand activity. The significant increase of density in right part of frequency
range has contributed to mean frequency of oscillations rise by 5.3 Hz.
The vestibular apparatus stimulation induced a tendency of energy
indices diminution during lsl min after test. It was a repercussion of
decrease by l.4 fold of the density in range of 7-13 Hz and by 1.3 foldin range of 14-18 Hz. Conclusions. The less increase or decrease of
the energy indices and of oscillation density in frequency intervals
modulated by central oscillator in old persons presumably indicate infavor of a synaptic transmission inhibition due to a durable activation ofsome neuro-humoral processes in the allostatic load amplification and to
an information transmembranic exchanging (receptors desensitization,“uncoupling” and “down regulation” phenomena).
Key words: physiological tremor, allostatic load.

129. THE EVALUATION OF THE PREDICTORS OF
PROGRESSION FROM MILD COGNITIVE IMPAIRMENTTO ALZHEIMER’S DISEASE
D.I.SCOREI1, M.C MITRAN1, O.ALEXANDRU2,
M.BĂNICIOIU1, A.M.GEORGESCU4, L.TĂTĂRANU1,
V.CIUBOTARU3, A.DRICU5
Department of Psychiatry, Neuropsychiatry Hospital, University of
Medicine and Pharmacy, Craiova, Romania
2
Department of Neurology, Neuropsychiatry Hospital, University of
Medicine and Pharmacy, Craiova, Romania
3
Department of Neurosurgery, Bagdasar-Arseni Hospital, Bucharest,
Romania4
Medico Science SRL, Bucharest , Romania
5
Department of Biochemistry, University of Medicine and Pharmacy,
Craiova, Romania

1

Objective: The main purpose of our work was to determine theoccurrence of neuropsychiatric symptomatology and its relation to
future development of Alzheimer’s disease (AD) in persons withand without mild cognitive impairment (MCI). Method: We followed
185 persons with no cognitive impairment and 47 persons with MCI(amnestic and multidomain), ages 70-80 , for 3 years. Three types ofneuropsychiatric symptoms were assessed at baseline: mood-related
depressive symptoms, motivation-related depressive symptoms, andanxiety-related symptomatology. At 3-year follow-up AD was diagnosed
according to Diagnostic and Statistical Manual for Mental Disorders-III-R
criteria. Results: Psychiatric symptoms occurred more frequently inpersons with MCI (36.2% mood, 36.2% motivation, and 46.8% anxiety
symptoms) than in cognitively intact elderly individuals (18.4% mood,
13.0% motivation, and 24.9% anxiety). Of persons with both MCI andanxiety symptoms, 83.3% developed AD over follow-up versus 6.1%
of cognitively intact persons and 40.9% persons who had MCI withoutanxiety. Among persons with MCI, the 3-year risk of progressing to AD
almost doubled with each anxiety symptom (relative risk [RR] 1.8 [1.2
to 2.7] per symptom). Conversely, among cognitively intact subjects,
only symptoms of depressive mood were related to AD development(RR 1.9 [1.0 to 3.6] per symptom). Conclusions: The predictive validityof mild cognitive impairment (MCI) for identifying future Alzheimer’s
disease (AD) cases is improved in the presence of anxiety symptoms.
Mood-related depressive symptoms (dysphoria, suicidal ideation, etc.)
in preclinical AD might be related to the neuropathologic mechanism, as
they appear preclinically in persons both with and without MCI.

130. EFFECTS OF AORTIC CONSTRICTION ON
THE ELECTRICAL AND MECHANICAL ACTIVITY
OF RAT PAPILLARY MUSCLESCRIDON ALINA1, SERBAN R.C.2, PERIAN M.1, BARBAT GH.1,
DOBREANU D.1
Department of Physiology, University of Medicine and Pharmacy, Targu
Mures, Romania
2
Cardiology Clinic, Institute of Cardiovascular Diseases and Transplant,
Targu Mures
1

Background: As response to a variety of mechanical, hemodynamic,

Fiziologia ‑ Physiology  2009 Supplement41Official Journal of the Romanian Society of Physiological Sciences
hormonal stimuli, the myocardium adapts through the hypertrophy ofindividual muscle cells. The mechanisms involved in pathological left
ventricular hypertrophy (LVH) are far from clear. The results of somestudies were interpreted as suggesting that excitation-contraction coupling
could be altered in this context. Aim: The purpose of this study was tounderstand the electro-mechanical mechanisms involved in pathological
LVH. Methods: Thirty Wistar rats were randomly assigned into two groups:
control group and the group with aortic constriction. The study group was
subjected to surgical intervention in order to accomplish the abdominal
aortic constriction. After 4 weeks, both trained and sedentary rats wereanesthetized and then sacrificed. Several electrical and mechanicalparameters were measured. To investigate the possible role of analteration in excitation-contraction coupling in cardiac hypertrophy, we
compared simultaneously recorded mechanical and electrical activityof normal and hypertrophied papillary muscles. Results: Heart weights
was significantly greater for the animals with aortic constriction by 38.7%,
p<0,0001. The action potential (AP) recorded from the operated rats was
substantially longer than that recorded from the control group: APD90(136.9±0.4msec vs 75.4±1.1msec, p<0,0001), APD75 (78.1±0.5msec
vs 45.4±0.7msec, p<0,0001), APD50 (26.1±0.6msec vs 21.1±0.6msec,
p<0,0001) and APD25 (14.4±0.4msec vs 13.1±0.8msec, p<0,0001).
The amplitude of contraction was greater for the animals with aortic
constriction (880.1±1.7mN vs 485.1±2.3mN, p<0,0001), same as
TAC (105.1±0.2msec vs 126.1±2.8msec, p<0,0001) and T1/2R(220.1±1.4msec vs 126.1±2.8msec, p<0,0001).Conclusions: For theoperated rats we obtained a significant prolongation of all AP phases.
Still, the most important prolongation was based on the changes in the
repolarization. This changes could explain the occurrence of cardiac
arrhythmias in the presence of maladaptive LVH. The longer duration ofcontraction in rats with LVH may be related to the longer APD observedin hypertrophied muscles. Since the maximum tension development in rat
ventricular muscle is affected very little by depolarization lasting beyonda certain limit, it is possible that once APD reaches that certain length,
further lengthening of the AP does not influence tension development.
This could account for the absence of correlation between APD andparameters of contraction in hypertrophied muscles.

131. ENDOTHELIUM-DEPENDENTVASODILATION: MEHANISMS FOR FUNCTIONSAND DYSFUNCTIONSSERBAN IL, COVIC AC, BILD W, SERBAN DN“Grigore T. Popa” University of Medicine and Pharmacy, Iasi,
Romania
Endothelium-dependent relaxation (EDR) of vascular smoothmuscle (VSM) is based on complex signaling. Our goal is to analyze
how such signals are used in EDR induced by vasoactive agents, andin flow-dependent and propagated vasodilation, while mechanisms
vary with species, age, sex, hormonal status, vascular bed/caliber, thevasodilating factor and vascular tone, oxidative stress and pathologicalconditions. The major relaxing factors derived from endothelium, e.g.
nitric oxide (NO), prostacyclin and hyperpolarizing factors (EDHF: epoxyeicosatrienoic acids, anandamide, K+, H2O2, natriuretic peptide C) havemultiple actions, but all induce VSM hyperpolarization by activating K+
channels, thus limiting influx of activator Ca2+ through L-type channels.
Moreover, membrane potential changes propagate in vascular walls
via gap junctions. We review elements of progress in understanding42

the mechanisms of endothelial functions and dysfunctions, recently
dicussed together by contributors in the field, as follows: intercellular
hyperpolarization and vasodilation (Garland CJ); divergent roles for Cadependent K-channel types (De Wit C); TRP channels in cardiovascularfunction (Nilius B), molecular organization of store-operated calcium
channels (Barritt GJ, Serban DN); heterogeneity of calcium responses
to ACh in endothelial cells (Beny JL); intraluminal pressure, endothelins,
COX isoforms, and functions of vascular angiotensin receptors (Koller
A, Bild W); NOX homologues, reactive oxygen species, and vascularbiology (Touyz RM); impairment of EDR by oxidative stress (Forstermann
U); variable profile of mechanisms that ensure endothelium-dependent
vasodilation in normal conditions and diabetes (Triggle CR, Serban IL);
endothelial dysfunction, stiffness and calcifications as arterial changes
in renal disease (Zoccali C, Covic AC). Supported by Romanian Grant
PN2-IDEI-PCE/ID-1156/2007-2010.
Key words: endothelium, ion channels, angiotensin, oxidative stress,
chronic renal disease.

132.CHANGES OF THE FIBRIN CLOT’S RESISTANCE
TO BREAKAGE IN DIABETIC PATIENTS WITH
ISCHEMIC STROKEVERONICA SFREDEL1, SUZANA DANOIU2, MADALINA MANEA3,
ANDRA POROJNICU4, SMARANDA MITRAN1, A.T. BALSEANU1,
H.TRAILA5
Department of Physiology, University of Medicine and Pharmacy,
Craiova2
Department of Physiopatholgy, University of Medicine and Pharmacy,
Craiova3
University of Medicine and Pharmacy, Craiova
4
Student - University of Medicine and Pharmacy, Craiova
5
Pitesti University

1

Object: The object of this work was to check if in diabetic patientswith ischemic stroke,there are other qualitative alterations of the fibrin
clot ,besides the quantitative changes of the factors involved in thehemostasis. We decided to use the “fibrinoresistometric” method inorder to investigate one qualitative parameter of the fibrin clot, namely
the fibrin clot’s resistance or elasticity.
Material and methods: We determined the fibrin clot’s resistanceto breakage at 21 diabetic patients( 21 males,10 females) who
suffered from ischemic stroke.These measurements were performedon venous integral blood in the first 60 minutes after the brain attack
was diagnosed. In parallel we investigated the fluid clotting equilibriumusing the temporal methods: PT (prothrombine time), INR, APTT andthe plasma concentration of fibrinogen. The witness group contained 20
diabetic patients with no previous records of ischemic stroke
Results: The normal values for the fibrinoresistometric method were200-300 FM with an average of 250 FM. .The fibrinoresistometric valuesof the diabetic patients were 24, 18 % higher than the normal values ofthe method. At the diabetic patients with ischemic stroke these values
were 29.7% higher than those of the witness group.The clot timing
had normal values for the two groups of patients and the fibrinogenconcentration was significantly risen in the ischemic stroke group.
Conclusions: While the clot temporal tests remain normal theparameter we suggested for the qualitative exploration of the fluid
clot equilibrium proves to be much more sensitive indicating thehypercoagulability tendency in the tested patients.

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Official Journal of the Romanian Society of Physiological Sciences
Key-words: fibrinoresistometric, stroke, diabet. CNCSIS PROJECT
562/2008.

133. THE FIBRIN CLOT’S RESISTANCE TO
BREAKAGE AT DIABETIC PATIENTS TREATED
WITH ASPIRIN.
VERONICA SFREDEL1, CATALINA PISOSCHI2, VIORELA
ENACHESCU3, A.T. BALSEANU1, SMARANDA MITRAN1, IRINA
PREDA4, ANCA RIZEA4Department of Physiology, University of Medicine and Pharmacy,
Craiova2
Department of biochemistry, University of Medicine and Pharmacy,
Craiova
3 rd
III Medical Clinic, Clinical Hospital no.2
4
Student - University of Medicine and Pharmacy, Craiova

1

Our purpose is to encounter possible changes in the fibrin clot’s
resistance to breakage at diabetic patients with cardiac risk that werepreviously treated with aspirin. Materials and method . We determined thefibrin clot’s resistance to breakage at a group of 31 diabetic patients withan average age of 59.35 , 14 females and 17 males, before and during thetreatment with aspirin ( aspenter) in a dose of 70mg/day. Simultaneously,
we investigated the fluid clotting equilibrium using the acknowledged
temporal methods: PT (protrombin time), INR, APTT and the plasmaticconcentration of fibrinogen.These measurements were performed on
venous integral blood. Results. Before starting the antiagregant treatment,
the average of the fibrinresistometric values was higher than the normal
values ( 386 FU). The highest values were encountered at the patients
suffering from diabetes for more than 10 years. After a week of aspirin
administration, the clot’s resistance dropped ( 307 FU – which meansa decrease of 20,5%). The highest values were obtained at patientswith diabetes for more than 10 years. After a month from the beginningof the treatment, the resistance didn’t suffer any significant changes
in comparison with the first determination. The INR was insignificantly
modified, without reaching the area of hipoclotting. Conclusions. The
determination of the fibrin clot’s resistance to breakage is useful inestablishing the effects of the antiagregant treatment and the necessary
doses for reaching the area of normo- or hipoclotting
Key-words: resistance, fibrin clot, antiagregant. CNCSIS PROJECT
562/2008

134. OXIDATIVE STRESS AND BRAIN AGEINGDIANA SFREDEL1, ANCA RIZEA2, B. CATALIN2, MARIA
IANCAU3
Emergency University Hospital, Bucharest
Students, University of Medicine and Pharmacy, Craiova
3
Department of Physiology, University of Medicine and Pharmacy,
Craiova1

2

Brain ageing is a complex multifactorial process, which assumes
that programmed altering of the genetic material is associated with
epigenetic factors, which accelerate the process. Through a concealed
action, the epigenetic factors cause irreversible damage at different levelsof the biologic system starting with the DNA and the gene expression. A

Fiziologia ‑ Physiology  2009 Supplement

major source of DNA lesions is the oxidative stress produces by various
reactive species of oxygen. Oxidative deamination, oxidation of the bases
from the DNA strands with their consequent break, base alkylation orhydrolysis take place. Furthermore, because of the replication errors,
damaged bases are wrongly inserted or omitted from the DNA strands.
The damages, which are in the beginning chemical, are expressed byphysical anomalies: one or both DNA strands are broken, telomeres are
lost, oncogenous residues are attached. Consequently, the structureof the DNA molecule is altered, which can lead to the impossibility totranscript the affected gene. The effects induced by an excess of oxygenand nitrogen reactive species leads to a decrease in cognitive and motor
functions, as well as to the onset of degenerative diseases such as
Alzheimer dementia, Parkinson syndrome etc. In these conditions, free
radicals are not a unique factor, but are most definitely contributing.
Key words: Brain, ageing, oxidative stress.

135. AUTOIMMUNE MANIFESTATIONS IN SYSTEMICRHEUMATIC DISEASESISABELA SILOSI1, M. COJOCARU2, OURANIA DRAKOULOGONA4,
V. BICIUSCA1, ALICE GAMAN2, IRINA ANCA EREMIA3, V.
PADUREANU1, SUZANA ROGOZ1
University of Medicine and Pharmacy, Craiova
Titu Maiorescu” University, Faculty of Medicine, Bucharest,
3
Emergency Hospital, Bucharest
4
Hospital Patra, Greece

1

2

Objectives.The aim of this paper is identification of specificautoantibodies in some rheumatic diseases. We studied the incidenceof serum antinuclear antibodies (ANA) and specific autoantibodies
to nuclear antigens in patients with autoimmune disorders: systemic
lupus erythematosus (SLE), mixed connective tissue disease (MCTD),
systemic sclerosis (scleroderma) and rheumatoid arthritis(RA) andSjogren syndrome(SS). Material and methods. Our study included anumber of 45 cases with systemic rheumatic diseases. Based on theclinical manifestation the patients were allocated to one of the following
five groups: 16 patients with SLE, 7 patients with MCTD, 7 patients withscleroderma,15 patients with RA and 10 patients with SS. ANA wereidentified by indirect immunofluorescence test. For identification of
specific-autoantibodies (anti-ds DNA, Sm, RNP, SS-A(Ro), SS-B(La),
Scl-70 was utilized Enzyme Linked Immunosorbent Assay. Results.
Incidence of ANA was 96% in SLE, 84% in MCTD, 68% in scleroderma,
39% in RA. The combination ds DNA-RNP-Sm was detected in mostpatients with SLE. Patients with MCTD had high levels of anti-RNP
autoantibodies. Scleroderma patients with severe organ involvement
were associated with autoantibodies to enzyme topoisomeraza-1 (antiScl-70). Autoantibodies anti-Ro and anti-La are found in patients with
SLE, SS and RA. Conclusions. The detection of specific autoantibodies
plays an essential role in differential diagnostic of autoimmune diseases.
The interpretation of any autoantibody test should be made in the context
of a history, clinical examination and interpretation of the potential false
positive results.
Key words: autoantibodies, autoimmune disorders, rheumatic
diseases.

43

Official Journal of the Romanian Society of Physiological Sciences

136. IMMUNE DYSFUNCTIONS IN PATIENTS WITH
CONGESTIVE HEART FAILURE
ISABELA SILOSI1, M.COJOCARU2, INIMIOARA MIHAELACOJOCARU3, IRINA ANCA EREMIA4, C.A. SILOSI1, RODICAMUSETESCU1, V. PADUREANU1, ALICE GAMAN2
University of Medicine and Pharmacy, Craiova,”Titu Maiorescu” University, Faculty of Medicine, Bucharest
3“Carol Davila” University of Medicine and Pharmacy, Bucharest
4
Emergency Hospital, Bucharest

1

2

cardiovascular prevention to these subjects, as well as the optimizingof a treatment according to the observed risk factors are respected. In
the study have been included 130 patients aged between 58-65 years,
from which 52% are men. The inclusion criteria has been the lack of any
symptom in the patients with dilated cardiomyopathy found after some
general periodic check-ups. The evaluation on inclusion and the threeconsecutive evaluations consisted in a clinical and paraclinic examination.
The results of the present study have offered a comprehensive image
over the identification measures of the increased high cardiovascular risk
factors, in order to remit their influence on the evolution of affections
Key words: cardiovascular risk factors, cardiovascular prevention,
asymptomatic.

Background and objectives. Immune dysfunctions have been
postulated to play a role in the pathophysiology of chronic heart failure.
Our study followed the serum evaluation of inflammatory mediators(cytokines, C reactive-protein, C3) in patients with congestive heart
failure(CHF), to estabilish the implication in the pathogenesis of thedisease and their quality as markers of clinical evolution. Methods: Sera
from 20 patients with CHF(belonging to I-IV NYHA functional classes)
and from 16 healthy persons were assayed for interleukin-6 (IL-6),
IL-10, tumor necrosis factor-α (TNF- α) by using ELISA comercial kits.
These cytokines were related to serum levels of component C3 and
CRP, wich were determined by single radial immunodifusion techniqueand ELISA. Results. Levels of cytokines IL-2, IL-6,TNFα were elevated(31,78 ±23,02, 10,88 ±3,70, respectively 9,58 ±5,34 pg/ml) in patients
versus the concentrations of healthy persons. The frequence of elevatedlevels of IL-6 has been correlated with NYHA class. High plasma levelof interleukin-6 was an important predictor for prognostic in patientswith congestive heart failure. The serum C3 concentrations havebeen significantly higher in patients compared with control group (t=0,0048), sustained the role of complement system in the developmentof inflammatory heart disease This study supports the involvement ofinnate immunity in the pathogenesis of CHF. Our findings suggest thatthe complement may be added to the list of possible therapeutic targets in
CHF and that future studies with specific complement inhibitors might beof interest in this disorder.The serum concentration of C-reactive protein
was higher than normal in 13 (65%) patients and directly related to theseverity of disease. Conclusions. The investigated markers (cytokines,
CRP, C3) should participate at the appearance of lesions and/or at
the progression of disease sustained the involvement of these soluble
factors in pathogenesis and physiopathology of CHF.
Key words: cytokines, inflammatory mediators, congestive heart
failure.

138. THE IMPORTANCE OF THE EFFORT TEST IN
DISCOVERING ISCHEMIC CARDIOPATHY137. PREVALENCE OF CARDIOVASCULAR RISKFACTORS IN PATIENTS WITH IDIOPATHIC DILATEDASYMPTOMATIC CARDIOMYOPATHYCOSTIN STEFAN, DUMITRU BULUCEA, CORINA STEFAN,LARISA IOANA VASILE, VASILE STEFANCORINA STEFAN, MARIA IANCAU, COSTIN STEFAN, V.
STEFAN
University of Medicine and Pharmacy of Craiova
The main objective of this study is represented by the determiningof cardiovascular risk factors and the realizing of an objective imageof the cardiovascular prevention in patients with idiopathic dilated
asymptomatic cardiomyopathy. The purpose of the present study isto evaluate the measure in which the recommendations concerning

44

CORINA STEFAN, MARIA IANCAU, COSTIN STEFAN, LARISAIOANA VASILE, V. STEFAN
University of Medicine and Pharmacy of Craiova
The effort test is widely used both in a diagnostic purpose as
well as in the evaluation of cardiovascular affections and involves theregistration of an EKG before and after physical effort, both on a roller
carpet or using an ergometric bicycle. The test resides in the progressive,standardized increase of the effort, continuously monitoring the EKG,
the symptoms and the arterial tension of the patient. Performance islimited and the test is being interrupted when signs of thoracic discomfort
appear, suffocation sentiments, dizziness, fatigue, the ST segment underdeviation more that 2 mm, the lowering of systolic blood pressure under
10 mm Hg and the appearance of some ventricular tachyarrhythmia. The
effort test tends to discover any limitation of effort performances andto establish the relationship between thoracic discomfort and the EKG
signs typical to myocardial ischemia. This test may be positive, falsely
positive or negative. The negative result does not exclude ischemic
cardiac disease.
Key words: diagnostic purpose, evaluation of cardiovascularaffections, EKG, arterial tension.

139. PHYSIOPATHOLOGICAL MECHANISMS
OF PORTAL HYPERTENSION IN HEPATIC
CIRRHOSIS

University of Medicine and Pharmacy of Craiova
It is well known that during hepatic cirrhosis the portal hypertension
is a consequence of the increase of intrahepatic vascular resistanceassociated with splanchnic circulatory hyper kinesis; it relates to anincrease of the debit in portal vein territory and to a splanchnic arterial
vasodilatation. Portal hypertension is emphasized by the circulatory
hyperkinesis. In splanchnic hyperkinesias mechanism are involved a lotof factors more or less associated: vasodilatation caused by the actionof some neurohumoral or paracrine substances; portosystemic collateralcirculation; hepatocellular disease; hypovolemia. The objective of thepresent study is to bring informations which emphasize the action of

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Official Journal of the Romanian Society of Physiological Sciences
endothelin in the increase of intrahepatic vascular resistance but also
the role of nitrogen monoxide in splanchnic arterial vasodilatation.

140. CLINICAL AND METABOLIC INDICES INOBESE HYPERTENSIVE PATIENTS IN FUNCTION
OF DEGREE OF INSULIN RESISTANCESTRATULAT SILVIA1, LYSYI LEONID1, ZOTA LARISA2, PROTOPOPSVETLANA1, AMBROS ALA1
Department of Biochemistry and Clinical Biochemistry, State Universityof Medicine and Pharmacy “Nicolae Testemiţanu”, Chişinău, Moldova
2
Department of Human Physiology, State University of Medicine andPharmacy “Nicolae Testemiţanu”, Chişinău, Moldova

1

Objective: The aim of this study was to investigate the clinicaland metabolic indices in obese hypertensive patients. Design andmethods: Insulin sensitivity was evaluated by Caro’s index (glucose to
immunoreactive insulin ratio) in basal state and at 2 hours during oral
glucose tolerance test (OGTT). 114 obese hypertensive patients weredivided into two groups in function of the Caro’s index in basal state – 39
insulin resistant patients (Caro’s index < 6) and 75 patients with normal
insulin sensitivity (Caro’s index > 6), and into two groups in function of
the Caro’s index at 2 hours during OGTT – 92 insulin resistant patientsand 22 patients with normal insulin sensitivity. Results: In the insulin
resistant patients, evaluated by both criteria, body mass index (p<0,001),
waist circumference (p<0,001), waist to hip ratio (p<0,001), systolicblood pressure (p<0,05), and diastolic blood pressure (p<0,01) werehigher than in the patients with normal insulin sensitivity. Also in thesepatients were detected elevated triglyceride (p<0,01), total cholesterol(p<0,05), low-density lipoprotein cholesterol levels (p<0,05), atherogenic
coefficient (p<0,05), and diminished high-density lipoprotein cholesterollevels (p<0,05), compared with the patients with normal insulin sensitivity.
Conclusion: Our study showed that basal Caro’s index allows to identify
insulin resistance in 34,2% cases, while the investigation of this index
at 2 hours during OGTT allows to identify it in 80,7% cases. Our results
indicate that insulin resistance substantially contributes to the evolutionof the metabolic syndrome and to the atherogenic dyslipidemia foundin the obese hypertensive patients.
Key words: insulin resistance, metabolic X syndrome, hypertension,
obesity.

141. EXPERIMENTAL STUDY ON THE ANTIOXIDANT
EFFECTS OF POLYPHENOLS IN EHRLICH ASCITESCARCINOMA
S.SUCIU1, A.MURESAN1, D.DAICOVICIU1, S.CLICHICI1,
I.D.POSTESCU2, A.CEBAN1
1

this study was to estimate the antioxidant effects of a red grape seedextract in Ehrlich ascites bearing mice. Materials and methods: Male
Swiss mice were used in the experiment: Group I (controls): EAC-bearing
mice, Group II: EAC-bearing mice + doxorubicin (dox) i.p. the days +1
and +4;group III: EAC-bearing mice + dox i.p. in the days +1 and +4
and GSE i.p. in the days 0, +1 and +4. Mice were sacrificed in day 10
and blood, liver and heart samples were obtained. Malonaldehyde(MDA) and total sulphhydryl groups (SH) were measured in plasmaand organ homogenates. Results: while tending to decrease total
SH groups, doxorubicin significantly increased plasma (p<0.02), liver(p<0.01) and heart homogenate (p<0.05) MDA levels as compared tocontrols. Administration of polyphenols slightly increased the total SH
and significantly decreased the intensity of oxidative stress in plasma((p<0.05) and liver (p<0.05), but not in the heart. Conclusion: the redgrape seed extract we used seems to have different antioxidant effects,
depending on the organ.
Key words: polyphenols, antioxidant, Ehrlich ascites.

142. SOME IMMUNOLOGICAL ASPECTS OF THE
VASCULAR ENDOTHELIUM
ANDREEA ŞTEFĂNUŢI
„Titu Maiorescu University“, Bucharest, Medicine Student
The endothelium is the thin layer of cells that line the interior surfaceof blood vessels, forming an interface between circulating blood in thelumen and the rest of the vessel wall. Complex physiopathologicalstresses can alter regulatory functions of the endothelium. Adhesionof leukocytes to the endothelium is one of the most important eventsin the reaction to all forms of injury. Interaction of endothelial cells withactivated leukocytes is associated with defective endothelium-dependent
vasodilation, increase in vascular permeability and in activation of thecoagulation cascade. Many leukocyte products, including reactive
oxygen species, superoxide and inflammatory cytokines, impair
endothelial function and create the potential for a positive feedback
loop between inflammation and coagulation. Research reports haveshown a role for these molecules in a number of pathological processes
including atherosclerosis, transplant rejection, septic shock, late phase
hypersensitivity reactions and reperfusion injury. Through its interactions
with leukocytes and other mediators, it is central to the development ofinflammatory foci and to lymphocyte trafficking around the body. Tissue
injury may arise here as a result of abnormal inflammatory or immune
responses. The potential for such injury to contribute to autoimmune
disease is discussed, particularly in relation to autoimmune vasculardisease. Understanding the central role that endothelial function has inmaintaining immune homeostasis has lead to the concept that endothelial
dysfunction may also play an important role in autoimmune disease.
Key words: endothelial cells, endothelial dysfunction, immunologicalaspects.

“I.Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca“I.Chiricuta” Oncological Institute, Cluj-Napoca

2

Background: Demonstration of the implication oxidative stress incarcinogenesis entail finding of counteract solutions of reactive oxygen
species effect. Much studied in last decade have been the natural
products possessing antioxidant activities. One aspect of antioxidant
administration is that this could affect both antineoplastic efficacy andthe development of side effects of anticancer drugs. Aim: The aim of

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45Official Journal of the Romanian Society of Physiological Sciences

143. CORRECTION BY ALGAL REMEDIES OF LIPIDPEROXIDATION DISTURBANCES CAUSED BYLIVER OSTEOPATHY
OLGA TAGADIUC1 , GUDUMAC V. 1 , REVA V. 2, RUDIC V. 3
State University of Medicine and Pharmacy “N.Testemiţanu”, R.
Moldova2
State University, R. Moldova
3
Microbiology Institute of the Academy of Science, R. Moldova

1

The aim of our research was to assess the efficiency of algal
remedies (BioR) in the correction of lipid peroxidation (POL) disturbancesin experimental liver osteopathy induced by carbon tetrachloride. In the
study were involved 32 rats that were divided in 4 groups – control (1),rats with liver osteopathy induced by carbon tetrachloride administration(2), rats with liver osteopathy treated with different doses of BioR – 1mg/kg (3) and 2 mg/kg (4) body weight. Lipid peroxidation was evaluatedby the assay of total oxidant activity, the amounts of initial products oflipid peroxidation – lipid hydroperoxides, dienic conjugates and carbonylcompounds in hexane and hydroalcoholic phases, and of malonic
dialdehyde. The antioxidant protection was evaluated by measuring
the total antioxidant activity. BioR (1 mg/kg) increased significantly
POL in the hexane phase and diminished it in the hydroalcoholic one,in comparison with the rats with liver osteopathy and control one; theamount of malonic dialdehyde and total oxidant activity didn’t change,
but the total antioxidant activity increased considerably (7%, p < 0,05).
Double dose of BioR (2 mg/kg) also didn’t influence the amount of malonic
dialdehyde and total oxidant activity, but increased more significantly
the level of total antioxidant activity (11%, p < 0,0005) and reduced tonormal values the concentrations of the lipid hydroperoxides, dienic
conjugates and carbonyl compounds in hexane and hydroalcoholic
phases. Thereby, the experimental data shows the indisputableantioxidant effect of the algal remedy BioR in 2 mg/kg dose on bone inexperimental liver osteopathy.
Key words: liver osteopathy, lipid peroxidation, algal remedies.

144. ON THE CENTRAL FATIGUE AND NONINVASIVEMONITORING OF NEUROMUSCULAR FATIGUEMIHAI TARATA
Medical Informatics, University of medicine and pharmacy of Craiova
The paper details the central component of neuromuscular fatigue
and provides synthesis of the concept and technical possibilities ofmonitoring the neuromuscular fatigue via the electromyogram and themechanomyogram, as signals intimately mirroring muscle activationand contraction mechanisms. The Fast Fourier Transform-based andWavelet Transform-based techniques are critically analysed, illustrated
and discussed, on the international arena and through original work.
Author’s original technique - the Area/Amplitude Ratio (Raa) - is also
comparatively discussed, as an alternative instrument in monitoring
the neuromuscular fatigue in the isometric and dynamic contraction important issue in real life activity - , e.g. work in difficult environments,
pilots in mission, difficult work in normal environments, sport.

46145. THE AIM OF PARACLINICSEXPLORATIONS IN DIAGNOSTIC AND
PROGNOSTIC OF OPHTALMOLOGICALDISEASELILIANA-CERASELA TATARU1, D. ZAHARIA2, ANA
STRATONE2
Department of Oftalmology – Health Authority Galati
The Faculty of Medical Bioengineering – UMF Gr. T. Popa Iasi
3
Function Testings – UMF Gr. T. Popa Iasi

1

2

Summary: In our study, the pacients (males and womans) withdiferent ophtalmologicaly disease, age: 58-73 years were clinicalexaminated: visual acuity (betwen 0,00 and 1,00), intraocular tension(value betwen 8 and 34 mmHg), anterior pole (with cataract, pseudofak,
pupilary reactions, open iridocorneal angle), posterior pole (with glaucoma
aspect, degenerative and vascular modified). All pacients has normal
psychophysical test. The laboratory paraclinical exploration indicate
modified of lipidique profile(15%), glucidic profile(12%), and inflamatory
aspect(10%) . The record of vizual evoked potentials (VEP) is a posibility
for diagnostic and monitoring of ophtalmologic affections, with an EB
Neuro Myto, LED, Flash pattern, variabily frequency, the coherent
mediation at 500 st, 1-100 frequency band, 500 ms data acquisition
equipment. Was detected changes of P100 wave with increase oflatency, decrease of amplitude and appearance forms (bifid, biphazic,
multiphazic), in dinamic quest using more records. Conclusion: The
record of visual evoked potential – P100 wave related with clinical,
laboratory and imagistic exploration maybe a approach of diagnostic
and prognostic, important to detect and treat ophtalmological diseasein early, preclinical stages.
Key words: ophtalmic affections, visual evoked potential, clinicaland paraclinical exploration.

146. PROLONGED BED REST AND SERUM
BIOCHEMICAL CHANGES IN HUMANCARMEN TATU1, RF TATU2, CARMEN BUNU1, DANIELAPUSCASIU3, FLORINA BOJIN1, F SCHNEIDER1
UMF Victor Babes Timisoara
Introduction: Prolonged immobilization stress induces blood serum
biochemical changes. The aim of this study was to evaluate the effectof immobilization stress on some serum biochemistry values. Materialand Method: We evaluated 14 patients free from any systemic disease,hospitalized because of lower extremities fractures. Blood was takenfrom patients after 1, 14, 28 days of bed rest and we determined: glucose
concentration, total cholesterol, triglyceride, fats, HDL cholesterol,VLDL
cholesterol and plasma cortisol. Results: At the second determination,
glucose concentration was significantly increase (p<0,05). A significant
positive correlation (r 0,68 p<0,01), was observed between glucose andcortisol values. Triglyceride were significantly increased (p<0,05), HDL
cholesterol significantly decrease (p<0,01), while VLDL significantly
increased (p<0,05) after 14 days. A negative correlation (r - 0,81 p<0,001)
between plasma cortisol and HDL cholesterol was observed. Conclusion:
The prolonged bed rest induced modification of the serum biochemistryvalues. These changes were more pronounced in the active people.

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Key words: immobilization, prolonged bed rest, biochemicalvalues.

147. LARGE CONDUCTANCE CALCIUMDEPENDENT POTASSIUM CHANNELS MEDIATEENDOTHELIUM-DEPENDENT RELAXATIONOF RAT SMALL MESENTERIC ARTERIES IN
ALLOXANIC DIABETESTUCALIUC ES, SERBAN IL, HURJUI L, BRANISTEANU DD,SERBAN DN“Grigore T. Popa” University of Medicine and Pharmacy, Iasi,
Romania
Although streptozotocin is more widely used to induce rodents
diabetes resembling the human insulin-dependent one, in this study weused alloxan to induce diabetes in rats, as previously described. Bothagents are toxic due to their cellular uptake via GLUT2 transporter andsubsequent pancreatic beta cells destruction, which involves alkylation and
DNA damage in the case of streptozotocin, but a free-radical-generating
cycle in the case of alloxan. Others have shown that renal arteries fromrabbits with alloxan diabetes do not present endothelial dysfunction,
but participation of K(ATP) to endothelium-dependent relaxation isprevented. Based on our previous observations regarding endothelial
dysfunction in this model, here we investigate the EDHF phenomenon
and participation of certain potassium channels. We used isometric
myography of rings (1 mm wide) from mesenteric artery and its first orderbranches, obtained from male Wistar rats (200-250 g). We used 0.01
mM glibenclamide to block K(ATP) and 10 mM tetraethylammonium toblock BK(Ca). We tested the effect of carbachol (100 nM to 0.1 mM) in
phenylephrine-precontracted rings, as global endothelium-dependent
relaxation and its EDHF component (in presence of 0.1 mM L-NAME and0.01 mM indomethacin). Endothelium-dependent relaxation in diabetic
animals was reduced, but EDHF was enhanced in diabetic vs. control.
Moreover, Endothelium-dependent relaxation in diabetic animals was
fully inhibited by TEA, in contrast with healthy controls, where K(ATP)are also involved. K(ATP) are not involved in the EDHF component
in either diabetic or control animals. Supported by Romanian Grant
CNCSIS-A1222/2007-2008.
Key words: alloxan, diabetes, endothelium, BKCa, resistancearteries.

148. Clinical and electroencephalographic
correlations in focal epilepsy
Valerica Tudorica1, Denisa Pirscoveanu2, Smaranda
Mitran3
The Neurology Department of the University of Medicine and Pharmacy,
Craiova, Romania
2
The Neurology Department of the University of Medicine and Pharmacy,
Craiova, Romania
3
The Physiology Department of the University of Medicine and Pharmacy,
Craiova, Romania

1clinical aspects of focal epilepsy are correlated with the epileptogenic
focus revealed by the electroencephalography (EEG). Material andmethods. We have studied a number of 30 patients (20 men and 10
women) who met the requirements for being part of the study: a certain
diagnostic of focal epilepsy (based on clinical, EEG and imagistic criteria),
aged between 20 and 65 years, a period of 5 to10 years of illness. It
has been registered the dynamics of EEG to all patients.
Results. Depending of the clinical picture and imagistic investigations,they were diagnosed a number of 8 cases with frontal lobe epilepsy, 3cases of parietal lobe epilepsy, 3 cases of occipital lobe epilepsy and16 cases of temporal lobe epilepsy. For 13 cases (43,3%) it was founda discordance between the epileptogenic focus in EEG and the one
suggested by the clinical aspects. The epilepsy cases were distributed
as follows: 2 of frontal lobe, 1 of parietal lobe, 2 of occipital lobe and 8 of
temporal lobe epilepsy. There were encountered two different situationsin the patients with temporal lobe epilepsy: they had only one type of
seizure but they were tracked down 2 centers in EEG or the patients
had seizure polymorphism, but it was found only one focus in EEG.
Conclusions. The focal epilepsies involve, in many cases, polymorphism
both in EEG aspects and in clinical forms. The right correlation between
them may have important therapeutic consequences.

149. STUDY ON THE INFLUENCE OF BROME-BISDIMETHYL GLIOXIMATE COBALT (III) HYDRATEON HEPATIC MARKERS IN EARLY POSTNATAL
STAGE PIGSŢURCANU ŞT.1, GULEA A.2, MARIA BÎRCĂ2, DIANA BALAN1
1

The State Agricultural University of Moldova
The State University of Moldova

2

The functional activity of microelements is studied as a result of
their inclusion in metalorganic compounds with a well-defined shapeand structure. The activity of the microelements is increased significantly
when combined with organic substances, in comparison with the ionicstate these metals take in an organism. Therefore, our purpose wasto study the influence of the studied substance on hepatic functions bymeasuring several markers of hepatic metabolism in early postnatal
stage pigs. A total of 10 pigs, which were 7 days old, were divided in two
groups: experimental group and control group. We have determined thelevels of transaminases (ALT, AST) and alkaline phosphatase. Before
administering the studied substance, ALT levels (u/l) in the control groupwas – 28,1±3,7 (u/l), and after 7 days of treatment it was 30,8±6,76(u/l). In the experimental group, ALT levels were 30,84±6,76 (u/l). AST
levels in the control group were 29,9±2,2(u/l) prior to the experiment,
increasing 5,2 times after the 7 days of treatment (X=35,1±3,4(u/l).
The same tendency is noted in the experimental group in which an
7,2 times increase occurs (X=35,1±3,4(u/l). These variations are notsignificant (P>0.05). The levels of alkaline phosphatase decrease during
the experiment. Thus, in the control group, the blood levels of alkaline
phosphatase in pigs is 0,55±0,06 (μmol/l), and decreases 0,16 times(X=0,39±0,04(μmol/l), while in the experimental group decreases moreconsiderably from 0,57±0,09(μmol/l) to 0,34±0,03(μmol/l), (P<0.05).

Objectives. The purpose of the study was to detect how much the

Fiziologia ‑ Physiology  2009 Supplement

47

Official Journal of the Romanian Society of Physiological Sciences150. ENDOTHELIAL OXIDATIVE STRESS INDUCEDBY SERUM FROM PATIENTS WITH SEVERE
TRAUMA HEMORRHAGELARISA IOANA VASILE, MARIA VRABETE, COSTIN STEFAN,
CORINA STEFAN
University of Medicine and Pharmacy of Craiova
Objective. Shock induces oxidative stress by ischemia - reperfusion
phenomenon. Endothelial cells are involved in the inflammatory response
and oxidative stress responsible for microcirculation impairment andorgan failure. We examined the potential of serum from patients to
induce in vitro reactive oxygen species production by cultured humanumbilical vein endothelial cells (HUVECs). Patients three groups werecompared: hemorrhagic shock trauma patients, isolated brain injured
patients and healthy volunteers. Methods. In the hemorrhagic shockgroup we sought a correlation between reactive oxygen species
production and severity of shock. Serum was separated and perfusedin an in vitro model of perfused HUVECs. Ex vivo reactive oxygen
species production was assessed by fluorescence microscopy using
dichlorodihydrofluorescein, an intracellular dye oxidized by H2O2. Results
are expressed in proportional change from baseline and normalized
by protidemia to control for variation related to hemodilution. Results.
Reactive oxygen species production by endothelial cells exposed toserum fro hemorrhagic shock patients (46,2+/- 24,9%) was significantly
greater than in those with brain injury (3,9+/-35,1%) and in healthy
volunteers (-6,8+/-5,8%). Conclusion. Serum from trauma patients withhemorrhagic shock induces reactive oxygen species formation in naive
endothelial cells which is correlated to shock severity.

151. OXIDATIVE STRESS AND REACTIVE OXYGENSPECIESLARISA IOANA VASILE, MARIA VRABETE, COSTIN STEFAN
University of Medicine and Pharmacy of Craiova
This article discusses different aspects concerning classification/
nomenclature, biochemical properties and pathophysiological rolesof “reactive oxygen species” (ROS) which are pivotal to interpret theconcept of “oxidative stress”. In vitro studies in both the prokaryotes
and eukaryotes clearly demonstrate that exogenous or constitutive andinducible endogenous sources of ROS together with cofactors suchas transition metals can damage virtually all the biomolecules. This
adverse chemistry is at the origin of structural and metabolic defects
that ultimately may lead to cell dysfunction and death as underlying
mechanisms in tissue degeneration processes. The same biomolecularinterpretation of aging has been proposed to embodies an oxidative
stress - based process and oxidative stress may virtually accompany all
the inflammatory events. As a consequence, ROS have proposed to playseveral roles in the pathogenesis of chronic - degenerative conditions,such as athero-thrombotic events, neurodegeneration, cancer, someforms of anemia, auto-immune diseases, and the entire comorbidity of
uremia and diabetes. Nowadays, the chance to investigate biochemical
and toxicological aspects of ROS with advanced biomolecular tools has,
if needed, still more emphasized the interest on this area of biomedicine.
These technological advancements and the huge information available

48

in literature represent in our time a challenge to further understand theclinical meaning of oxidative stress and to develop specific therapeuticstrategies.

152. THE ROLE OF INDUCIBLE NITRIC OXIDESYNTHASE INHIBITOR IN RESUSCITATIONOF HEMORRHAGIC SHOCK IN RATS WITH
ALCOHOLEMIAANATOLIE VISHNEVSKI
Departament of Pathophysiology, State Medical and Pharmaceutical
University „Nicolae Testemitanu”, Republic of Moldova
Background: Alcohol intoxication is associated with a high incidenceof traumatic injury, particularly in the young healthy population. The impactof alcohol intoxication on the immediate pathophysiologic response to
injury has not been closely examined. We hypothesized that acute alcohol
intoxication would aggravate the immediate outcome from hemorrhagic
shock by impairing homeostatic counterregulation to blood loss. This study
was designed to evaluate the systemic arterial pressure modification
within hemorrhagic shock (120 minutes) associated with alcoolemia
before and after resuscitation with inducible nitric oxide synthase inhibitor(Difetur). Methods: Animals were anesthetized with ether inhalation forabout 10 min. After anesthesia, a polyethylene catheter was inserted into
the femoral artery and connected to a pressure transducer to monitor themean arterial pressure (MAP). Another catheter was inserted into thefemoral vein for intravenous administration of drug. The blood volume
withdrawn was 30% of the total blood volume. EtOH + rats receivd a 3g/
kg dose of 20% ethanol intraperitoneally 60 minutes before hemorrhagie.
Difetur was administered inravenously 20mg per kg. Results: Mean arterialpressure decreased (-30% (p<0,05)) after withdrawal of 30% of total blood
volume from the femoral arterial catheter in rats. MAP stayed relatively
low during the 2 h after induction of HS. Compared with the HS group,
basal mean arterial pressure was significantly lower (-20% (p<0,05) )in alcohol-intoxicated rats (blood-alcohol concentration, 170 +/- 50 mg/dL). Difetur administered in animals with hemorrhagic shock reestablish
the level of MAP. Within the hemorrhagic shock, under condition withethanol intoxication, Difetur increases the level of MAP (+35% (p<0,05))
comparing with level of MAP in nitrated animals. Conclusions: These
results indicate marked alterations in the hemodynamic responses tohemorrhagic shock by alcohol intoxication. Inducible nitric oxide synthaseinhibitor ad ministration conteract hypotension induced by hemorrhagic
shock and alcohol acute intoxication.
Key words: hemorrhagic shock; ethanol intoxication; MAP.

153. BIOCHEMICAL ASPECTS OF THE HEMORRHAGICSHOCK ASSOCIATED WITH ALCOHOLEMIAIN CONDITION BEFORE AND AFTER RESUSCITATIONWITH DIFETUR
ANATOLIE VISHNEVSKI1, STELA TODIRASH1, OLEG GALBUR1,
NADIA TODIRASH2
Departament of Pathophysiology, State Medical and Pharmaceutical
University „Nicolae Testemitanu”, Republic of Moldova
Student of the 3 year of Medicine, State Medical and Pharmaceutical
University „Nicolae Testemitanu”, Republic of Moldova

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
Successful resuscitation in the hemorrhagic shock (SH) associatedwith the acute alcohol intoxication is directly correlated to the degree ofthe lesion of vital organs and development of the Systemic Inflammatory
Response Syndrome. The object of the represented study is todetermine activity of the blood enzymes, such as AST, ALT, Amylase,
Creatinkinase in the HS (120 minutes) associated with alcoolemia in
condition before and after resuscitation with Difetutr (inducible nitricoxide synthase inhibitor). Methods: Animals were anesthetized withether inhalation for about 10 min. After anesthesia, a polyethylene
catheter was inserted into the femoral. Another catheter was inserted
into the femoral vein for intravenous administration of drug. The blood
volume withdrawn was 30% of the total blood volume. EtOH + rats
receivd a 3g/kg dose of 20% ethanol intraperitoneally 60 minutes
before hemorrhagie. Difetur was administered inravenously 20mg per
kg. Results: The data demonstrate increasing of the blood enzyme
activity in the HS associated with alcoolemia: AST +130% (p<0,05),
ALT +85% (p<0,05), Amylase + 254% (p<0,05), Creatinkinase +80%
(p<0,05), in comparison with the animals animals than in controls. After
resuscitation with Difetur enzymatic activity was decreased: AST -85%
(p<0,05), ALT -74% (p<0,05), Amylase -175% (p<0,05), Creatinkinase-50% (p<0,05) on the contrary to non resuscitated animals.
Conclusions: The inducible nitric oxide synthase inhibitor (Difetur) has
manifested cytoprotective effect thus limiting the damage of cytoplasmatic
cell membranes in liver, heart, pancreas, muscle.
Key words: hemorrhagic shock; ethanol intoxication; AST; ALT;
Amylase; Creatinkinase.

155. COMPARISON BETWEEN BIOIMPEDANCEAND OTHER TECHINCAL MEANS IN ESTIMATINGCARDIAC OUTPUT

154. NONINVASIVE MONITORING OF CARRDIAC
OUTPUT DURING HYPOXIA BY BIOIMPEDANCE1DRAGOS VLAD, ADRIAN MACOVEI, ILIE CAPANU
National Institute of Aerospace Medicine, Bucharest, Romania
Introduction: Bioimpedance is a noninvasive method allowing
hemodynamical monitoring of cardiac output both in normal and sickpersons. Material and methods: We used a lot of 10 pilots, scanned
free from any disease, with a mean age under 25. We exposed them tohypobaric hypoxia up to 5500 m. At 5500 they had to take a stress test.
ECG, BIOIMPEDANCE, PULSEOXYMETRY were performed using a
BIOPAC device. Stroke volume was calculated using producer’s equation.
Results: Cardiac output and oxygen saturation varied in opposition
during ascent (as expected), but in concordance during post effort
compensation and descent. Correlation between stroke volume andheart rate as components of cardiac output are presented. As expected,
heart rate contributes more to compensation. Finally, a comparation
between smokers and non smokers are made. Discussion: During stress
test, the rate rate plays a more important role in compensation. Attitudeadaptation modifies both stroke volume and heart rate. Cardiac output
does not differ between smokers and non smokers but oxygen saturation
is well correlated (p=0.05). Conclusion: Study of bioimpedance data canprovide further means of estimating hypoxia resistance.
Key words: Bioimpedance, hipoxia, stress test, smoking

DRAGOS VLAD, ADRIAN MACOVEI, MIRELA ANGHELNational Institute of Aerospace Medicine, Bucharest, Romania
Introduction: Bioimpedance could be an effective way to monitor cardiac
output. Methods: A review of current studies involving bioimpedance ismade. Our experience with correlation between cardiac output measured
by bioimpedance and Doppler ultrasound is presented. Results: We
obtained a correlation coefficient of 0.72 (p=0.06), which approaches
the end values of correlation measured by physiological rated methods
like thermodilution. Conclusion: Bioimpedance is not precise enoughto measure stroke volume, but is good enough to estimate dynamic
tendencies and for clinical use.
Key words: cardiac output, bioimpedance.

156. The EEG in cerebral ischemia: howunderstanding basic mechanisms canhelp us interpret clinical data
Leon Zagrean1, Andrei Ilie2, Dragos Ciocan1,
Alexandra O. Constantinescu1, Ana-Maria Zagrean1,
Mihai Moldovan3, 1Center for Excellence in Neuroscience, Department of Physiology
II, “Carol Davila” University of Medicine and Pharmacy, Bucharest,
Romania2
Department of Experimental Psychology, Oxford University, UK
3
Division of Neuroscience and Pharmacology, Panum Institute, Universityof Copenhagen, Denmark

The electroencephalogram (EEG) is a brain monitoring techniqueused to assess brain function and prognosis in patients suffering from
cerebral ischemia. Global cerebral ischemia (GCI) suppresses the EEG
to an isoelectric line within seconds. When the blood flow is restored theEEG recovers slowly through a pattern of bursts of activity alternatingwith suppression periods. Usually a slow-recovering EEG is associatedwith a bad prognosis. Nevertheless, the patho-physiological mechanisms
underlying these EEG changes are not fully understood thus limiting
their interpretability. At a cellular level, when metabolic demands exceed
the energy supply (as during cerebral ischemia). In the brain, ATP is
rapidly metabolized to adenosine that can block excitatory synaptictransmission via A1 receptors. We investigated the role of adenosine
A1 receptor activation at the neuronal network level by monitoring theischemia-induced EEG changes using a 4-vessel occlusion model inrats. We found that: 1) adenosine is rapidly released during GCI and
accelerates the EEG suppression; and 2) a second surge of adenosine
occurs during reperfusion, fragmenting the recovery of EEG in burstsuppression patterns. Thus, A1R activation during cerebral ischemia
and reperfusion can profoundly disrupt EEG activity without detrimental
consequences, and this observation should be accounted for wheninterpreting ischemic EEG changes in the clinical setting. Moreover, as
the majority of brain’s energy is used for electrical activity, adenosine
by disrupting the neuronal networks might even reduce the metabolic
demands to an extent that promotes neuronal survival in conditions ofenergy restriction.
Key words: EEG, adenosine, rat, neuro-monitoring.

Fiziologia ‑ Physiology  2009 Supplement49Official Journal of the Romanian Society of Physiological Sciences157. CLINICAL AND PARACLINICAL STUDY IN ALOT OF TEXTILE-INDUSTRY WORKERSZGABARUS MIHAELA SIMONA, SIMONA GUSTI
University of Medicine and Pharmacy of Craiova
The authors have accomplished a complex clinical and paraclinicalstudy in a lot of 250 textile-industry workers (patients) of S.C. XESTER
S.A. Ramnicu Valcea, by age between 17 – 65, predominant female(70%), and from the urbane environment (90%). Entire lot of workers(patients) has been divided as follows: a lot with a length of service
under 2 years, and a lot with a length of service over 15 years. For
these lots have been determined: IMC (Body Mass Index), systolicblood pressure, diastolic blood pressure, ventriculare look, by anautomatic oscillometric tensiometer – model OMRON M5-1, and havebeen effected: electro-cardiogram with a HEART SCREEN / 60G – 12
standard derivations apparatus, Holter – EKG, echo-cardiography withSLEE 401M – MOD, 2D-MOD apparatus, laboratory tests (regulartests, glicemia, lipidic profile, serumal calcium), cervical and lumbar
radiography. From the anlaysis of the clinical and paraclinical tests inthe studied lot, comparable with a lot of 60 clinical and biological healthy
persons from the same category of age (office workers, nurses, workers

50

which don’t work in the textile-factory), we can remark the followings:
43,2% from the studied lot presented HTA (High Blood Pressure), 36% presented atrial rhythm disorders, the predominant modifications
occur in the lot with longer length of service. At 12 % has been traced
out the presence of a spasmophilia. Approximately 65% (64,8 %) fromthe studied lot presented cervical and lumbar spondylosis, fact which
can be explained by the work posture.

158. CD40-CD40L IMMUNOREGULATORY SIGNALIN ATHEROGENESIS.
ZOTA IE., MELNIC EUGEN, MUNTEANU ANDREI, UNTESCO
MAXIM
Departament of Morphopatology, State Medical and Pharmaceutical
University „Nicolae Testemitanu”, Republic of Moldova
Contact-dependent interactions of CD40 receptor and its ligand
CD40L are regarded as a stimulator of atheroma-associated cells. A new
T lymphocyte-dependent pathway of activation of immune inflammationin the vascular wall is discussed, which is presumably maintained by
self-regulation of antiinflammatory cytokine production.

Fiziologia ‑ Physiology  2009 Supplement

AUTHORS INDEXABSTRACTS

A. BALSEANU,
A. FILIP,
A. LUPESCU,
A. MUREŞAN,A. NAGY,
A. NESTIANU,
A. NEŞTIANU,
A. POPA-WAGNER,
A. STEPAN,
A.CEBAN,
A.DRICU,
A.M.GEORGESCU,
A.MURESAN,
A.SAULEA,
A.T. BALSEANU,
ADAM BEGU,
ADINA OLTEANU,ADRIAN BĂLŞEANU,ADRIAN MACOVEI,ADRIAN NEŞTIANU,ADRIANA BUCATĂ,ADRIANA G. FILIP,ADRIANA GHERBON,ADRIANA HABOR,ADRIANA HUREZEANU,ADRIANA MURESAN,ADRIANA MUREŞAN,ADRIANA PIRTE,
AIDA BONTEA,ALA DONICA,
Alexandra Floriana Rosu,
ALEXANDRA FLORIANA
ROŞU,Alexandra O. Constantinescu,
ALEXANDRU CROITORU,
ALEXANDRU D.,
ALEXANDRU LIVIA.,
ALICE GAMAN,
ALICE GUSTI,ALINA PÂRVU,
ALINA VENTER,AMBROS ALA,AMELIA GAMAN,
AMELIA GENUNCHEDUMITRESCU, 2, 3
AMZOLINI ANCA, 24
AMZOLINI M., 24

3
12,30
26
12, 30
12
5
38
3
7
44
40
40
32, 4440
41, 4226
4
3
27, 48
2, 11
7
18
19
22
23
18, 32
26
14, 36
6
26
39
39
48
7, 9, 161
1
42, 43
10 , 13
15
14, 36
44
19
2, 3
24
24

Fiziologia ‑ Physiology  2009 Supplement

Ana Dragomirescu,
ANA LUCA,ANA STRATONE,
ANA-MARIA BUGA,ANAMARIA KESE,
Ana-Maria Zagrean,
ANATOLIE VISHNEVSKI,
ANCA BADARAU,
ANCA BĂDĂRĂU,ANCA RIZEA,ANCA SIN,
ANDOR MINODORA,ANDRA POROJNICU,
ANDRADA BRATU,
ANDREA CISMAŞPRUTEANU,
ANDREEA PETRICA,
ANDREEA ŞTEFĂNUŢI,ANDREI GEORGESCU,
Andrei Ilie,
ANGELA ANTONESCU,ANGELA M. LAZAR,
ANGELO AZZI,ANGHEL S,
ANICA DRICU,ANTAL KÁROLY,ANTOANETA PUNGA,ANTONESCU ANDREEA,ANTONESCU ANGELA,
AR. BIRIŞ,AS BIRIS,AURA CIOBANU,AUREL POPA-WAGNER,
B. CATALIN,
B.MELNIC,BACALOV IU.,
Badea daniela,
BALDEA IOANA,BARBAT GH.,
BEKIM MALA,
BIANCA DOBRE,
BILD W,BÎLICI T.,
BOBCOVA SVETLANA,BOGDAN DIONISIE,
BOJIN MF,
BOTNARIUC NATALIA,
BRANISTEANU DD,

8
10
45
8, 36
9, 25
48
47
11, 12, 27, 34
4, 11
42
22
29
41
7
11
35
44
11
48
36
25
31
5, 6
16
34
11, 271
1
30
30
10, 138, 36
17, 42
40
13
30
3
40
79
15
41
13
5, 37
16
6
19
34, 4651

Official Journal of the Romanian Society of Physiological Sciences
BUBUIANU ANCA,BUNU C,C LOGIN,
C. EFREM,
C. FORTAN,
C. LOGIN,
C. MARGARITESCU,
C. POPESCU,
C. TAISESCU,
C.A. SILOSI,C.CATOI,
C.LOGIN,CAMELIA CAPUCI,CAMELIA CIACLI,CAMELIA DALAI,CAMELIA FOARFĂ,CAMELIA GURBAN,CARMEN BUNU,CARMEN CIUFU,CARMEN TATU,CASCO D.,CATALINA CIORNE,
CĂTĂLINA CIORNEI,CĂTĂLINA CIORNEIGRATIELA MIHAI,CATALINA PISOSCHI,CATALINA POIANA,
CEAMITRU N.,
CECILIA ADUMITRESI,CECILIA AVRAM,
CENAŞA VIORICA,
CEZAR GEORGE BADALE,CHIRIGIU L.,
CHRISTINE POEHLKE,CIPRIAN DALAI,CIUREANU IOANASTEFANIA,
CL. MĂRGĂRITESCU,CLAUDIA BORZA,
CLAUDIU CĂLIN,CLAUDIU MIRESCU,CODRUŢ ROMAN,COJOCARI L.,
COJOCARU ELENA,CONSTANTIN CĂRUNTU,CORINA SERBAN,
CORINA STEFAN,
CORINA TATOMIR,
52

36
6
12
17
17
26
3
17
19
43
12
32
8, 9
33
14, 36
7
20, 3333, 45
1, 24, 3845
13
11, 27, 34
4, 11
4
42
14
38
1, 24, 38
31
31
11
26
36
14, 3616
7
6, 7, 28, 29
929
19, 381312
10
7, 28, 29
43, 4718

COSGAREA RODICA,
COSTIN STEFAN,
COSTULEANU MARCEL,COVIC AC,CRISTIAN BODESCU,CRISTIAN OANCEA,
CRISTIANA SIMIONESCU,
Cristiana Vaduva,
Cristina Beznă,CRISTINA DOBRE,CRISTINA FARCAS,
CRISTINA FARCAŞ,CRISTINA PETRICA,
CRISTINA PETRICĂ,CRISTINA STAN,
CRIVOI A.,
CROITORI C.,CZIKER ROXANA ELENA,
D. ALEXANDRU,
D. GEORGESCU,
D. LUPU,
D. PIRICI,
D. ZAHARIA,
D.DAICOVICIU,
D.GEORGESCU,
D.I.SCOREI,
D.N. SERBAN,
DACIANA CARMENNISTOR,DAN NITA,DAN ŞTEFAN CIOROIANU,
DANA PLESCA,DANCESCU RUCSANDRA,DANIELA BADEA,DANIELA BĂDIŢĂ,DANIELA DINCă,DANIELA MOTOC,DANIELA PUSCASIU,DANOIU M.,DANOIU SUZANA,DEAK E,DECEA NICOLETA,
DENISA ENESCU BIERU,
Denisa Pirscoveanu,
DHAOU YOUSRA,DIANA BALAN,
DIANA CONSTANTIN,
DIANA DODONETE,

3
43, 4712, 16, 21
23, 412438
739
5
15, 1624, 38
1
35
2, 3
15
13
13
24
19
17
30
7
45
12, 32, 44
5
40
23
33
5
11
33
14
2, 3, 35, 39
4, 16
13
31
45
14
14
6
18, 249, 17, 39
46
14
46
16
16

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
DIANA OLTEANU,DIANA SFREDEL,DOBJANSCHI LUCIANA,
DOBREANU D.,
DOINA DAICOVICIU,
DOROFTEIU MIRCEA,DRAGAN BORIS,DRAGONU LIVIA,DRAGOS ALEXANDRU,
Dragos Ciocan,
DRAGOS POPESCU,DRAGOS VLAD,
DUMITRIU IRINA
LUCIANADUMITRU BULUCEA,DUMITRU FERECHIDE,DUMITRU SUPAC,ELENA PREDUŢ,ELENA TAINAAVRAMESCU,EMRI ZSUZSA,EREMIA IRINA,F SCHNEIDER1,
F. LANG,
F. ROMANESCU,FELICIA SFRIJAN,
FILIMON NICOLETA,FILIP ADRIANA,
FIRA–MLADINESCUOVIDIU,FLORIN ROMANESCU,FLORINA BOJIN,FLORINA CIORNEI,FRANCISC SCHNEIDER,
G. POPESCU,
G. RACA,GABRIELA LUPUSORU,GABRIELA NEGRISANU,GABRIELA TANASIE,GANENCO ANDREI,GAVRILIUC LUDMILA,GEORGE BOERU,GEORGESCU D.,GEORGETA MIHALAS,GEORGIANA CĂTĂLUI,GERMAINE SAVOIU,GERMINA COSMINA,
GHERBON ADRIANA,

18
42
1
40
18, 26
1
5
23
8, 9
48
27
27, 4812, 15, 16, 21, 27
43
11, 2724, 2513
2, 9, 25, 32, 39
34
4
45
26
38
20, 3321
3
29
39
33, 45
11
31
38
37
27
7, 28, 29
33
37
19
8
1
19
10
6
17
29

Fiziologia ‑ Physiology  2009 Supplement

GHERMAN I.,
GHERTA LEONID,
GODOROJA NADEJDA,GOTIA SMARANDALAURA,GOTIA SMARANDA
RODICA,GUDUMAC V.,
GULEA A.,GURBAN CAMELIA,GURZU BOGDAN,GUSBETH-TATOMIR P,
GUSTI ALICE,GUSTI SIMONA,
H. KAISER,
H. MATCAS,
H.TRAILA,HERMAN ANDREEA,HERMAN D,
HOGAS MM,HOGAS SM,
HUREZEANU DAN,HURJUI L,
I. HURJUI,
I.D.POSTESCU,
IANCAU MARIA,
IASMINA SAVESCU,ILEANA ION,
Ileana NEACĂ,ILIE CAPANU,
ILINCA IONELA AURELIA,
INIMIOARA MIHAELACOJOCARU, 43
IOANA JURCĂU, 24, 25
IOANA SISKA, 33
IOANA STEFANESCU, 34
IONELA IANCU, 5, 13, 38
IONELA LACRAMIOARASERBAN, 23
IOVANESCU DORINA, 24
IRINA ANCA EREMIA, 42,
43
IRINA PREDA, 42
ISABELA SILOSI, 42, 43
IULIA ALEXANDRA RISTEA,
38
IULIA BRUJAN, 7
Iulia tudorascu, 30

13
20
19
21
21
45
46
21
12, 15, 16, 21, 27
23
22
5, 22
26
28
41
24
6
22, 2323
23
46
2332, 441
33
1, 24, 38
5
48
22
42
53
45
5
60
42
9, 17, 27, 39, 40, 59,64
42
15, 64
74
30
41
4, 5
53

Official Journal of the Romanian Society of Physiological Sciences
J. MINNERUP,JEAN-MARC ZINGG,
JOANTA ADELA ELENA,JOSEFINE KAISER,
K. DIEDERICH,
K. STÖCKER,
KARDOS JULIANNA,
L. MOCAN,
L. ZAGREAN,
L.TĂTĂRANU,LARISA IOANA VASILELAURA GOTIA,LAURA GOŢIA,LAURA POPESCU,LAURENTIU COMAN,LAVINIA NOVEANU,LELIA SUSAN,
Leon Zagrean,
LEON ZĂGREAN,
LEONARD MADA,LIANA MICLE,
LIGIA RUSU,
LILIANA IONESCU,LILIANA NEOLACULICEANU,
LILIANA ROGOJAN, 18
LILIANA-CERASELATATARU,LISII LEONID,
LORA MOŞANU,LORA MOŞANU-ŞUPAC,
LOREDANA HANZU,LOREDANA HANZUPAZARA,
LOREDANA HURJUI,LUCIAN RADU NEAMŢU,LUCIANA DOBJANSCHI,
Lucica Rosu,
LUCICA ROŞU,LUISA GEORGIANABÂCĂ,LYSYI LEONID,
M. BADEA,
M. COJOCARU,
M. COTEANU,
M. GEORGESCU,
M. GHITA,
M. GIURGIU,

54

3
31
24
36
3
3
34
30
20
40
43, 4720, 331836
12
19
7, 28, 2948
10
38
14, 36
9, 39
38
33
18
45
19
26, 3131
1, 24, 38
38
23
32
36
39
2, 3, 39
10
5, 37, 44
2, 3, 35
20, 33, 42
10, 131720
20

M. MAHMOOD,
M.BĂNICIOIU,
M.C MITRAN,
M.COJOCARU,
MADALINA GOLIEANU,MAGDA BURAGA,MAGDALENA CISMAŞPRUTEANU,
MANOLE COJOCARU,
MANU ROXANA ELENA,
MARANDUCA MINELAAIDA,MARGINEAN CRISTINA,MARIA BÎRCĂ,MARIA CRISTINA BEZNA,
MARIA CRISTINA BEZNĂ,MARIA DINCA,
MARIA IANCAU,
MARIA IANCĂU,MARIA PUSCHITA,MARIA VRABETE,MARIANA ARTINO,
MARIANA MURESAN,MARINELA BEZNA,
MARIOARA NICOLETAFILIMON,
Marius Capra,
MARIUS CRISTIANNEAMŢU,MATEI V.,MELNIC EUGEN,MICLAUS VIOREL,MICLE LIANA,
MICLE OTILIA,MIHAI CAMELIA ANA,MIHAI IOAN,
Mihai Moldovan,
MIHAI TARATA,MIHALAS GEORGETA,MIHU MIHAELA,MIOARA ZAMFIR,MIRCEA DOROFTEIU,MIRCEA LUPUSORU,MIRELA ANGHEL,
MIRELA LUCIA CĂLINA,
MITRAN MARIACRISTINA,MOCANU TEODORA,

30
40
40
43
33
34
11
33
20
27
20
46
5
39
15, 21, 27
5, 8, 9, 17, 42, 43
2, 32
33
7, 47
4, 11, 27, 34
14
5
18
8
2, 11, 30, 32
13
49
24
1
1
15, 21, 27
16
48
45
29
36
11
14, 3627
48
9, 25, 39
824

Fiziologia ‑ Physiology  2009 Supplement

Official Journal of the Romanian Society of Physiological Sciences
MOHAMMAD SADEKASHOUR,MOLDOVAN REMUS,MONICA BRATU,MONICA DRAGOMIR,MUNGIU OC,
MUNTEANU ANDREI,MURESAN ADRIANA,MUREŞAN MARIANA,
N. CEAMITRU,
N. DECEA,NADIA TODIRASH,
NASTASIA GHEORGHITA,
NECHIFOR M,
NICOLETA DECEA,
NINA LIOGCHII,NINELA RADULESCU,NINELA RĂDULESCU,NOVEANU LAVINIA,
O. Zlatian,O.ALEXANDRU,OANA CHILIŞOIU,OANA COMAN,OANA MARIA NEAMŢU,OANA MARIA RICA,
OLEG GALBUR,
OLGA TAGADIUC,OPRISA C,ORBÁN-KIS KÁROLY,OTILIA MICLE,OURANIADRAKOULOGONA,
P. BADEA,PARVU ALINA ELENA,
PARVU MARCEL,PAUNESCU V,
PERIAN M.,
PETRESCU BOGDANCRISTIAN,
PETRESCU GHEORGHE,PETRICA BADEA,PETRIŞOR CISMAŞPRUTEANU,PIRTE ADRIANA,
PRODAN M.,
PROTOPOP SVETLANA,
R. KANDOLF,
R. MOLDOVAN,

14
24
7
4
34
49
3
1
1, 24
12, 3047
23
22
26
26, 31
24, 38
1
29
39
40
26
12
2
37
47
45
34
34
14, 36
42
5, 38
14
35
6
40
12, 15, 16, 21
21, 2723
11
1
1337, 4426
26

Fiziologia ‑ Physiology  2009 Supplement

R.TANASIE,RALUCA IURCOV,RALUCA PAPACOCEA,RAMONA BURLACU,RAMONA HANDA,RAMONA MOLDOVANJURCĂU,REVA V.,RF TATU,
RODICA M. ION,
RODICA MATEESCU,RODICA MUSETESCU,RODICA TOROK-OANCE,RUDIC V.,
S. BEZNA,
S.CLICHICI,S.FABRIKANTOVA,S.GHIBU,
Ş.SIMON,
S.SUCIU,
SAULEA AUREL,SAVOIU–BALINTGERMAINE,
SCOREI DARIA IULIA,SCRIDON ALINA,SECELEANU ANDREEA,SERBAN DN,SERBAN IL,
SERBAN R.C.,
SFREDEL VERONICA,SILVIA NICA,SIMONA CLICHICI,SIMONA GUSTI,SIMONA TUDORICA
MICU,
SISKA IR,SLATINEANU SIMONAMIHAELA,
SMARANDA GOTIA,
SMARANDA IOANAMITRAN,
SMARANDA MITRAN,
SMARANDA RODICA
GOTIA,
SMARANDA RODICA
GOŢIA,SORIN ARAMĂ,SORIN TALPES, 31ŞTEFAN ELENA, 23

19
14
4, 11, 27, 34
31
29
24, 2545
45
18
6, 7, 28, 29
43
18
45
5
12, 30, 44
40
32
30
12, 32, 44
5
29
8
40
24
22, 23, 34, 41, 46
22, 34, 41, 46
40
14, 2411
18, 266, 10, 13, 28, 49
17
6
12, 1633
112, 41, 42
20
18
7
64
8
55

Official Journal of the Romanian Society of Physiological SciencesŞTEFAN SORIN ARAMĂ,STELA TODIRASH,
STOICA B,
STRATULAT SILVIA,SUZANA DANOIU,SUZANA ROGOZ,
SZILÁGYI TIBOR,
T. BOCK,
T. DĂIANU,
T. MOCAN,
T.PAPACOCEA,TANASIE G,TARIG OMER ELAMIN,TATU C,TATU CA,TEODORA ROMAN,TRAIAN BOCU,
TUCALIUC ES,
Tudor- adrian balseanu,
TUDOR-ADRIANBĂLŞEANU,
ŢURCANU ŞT.,UNTESCO MAXIM,
V. BICIUSCA,
V. ERDELEAN,
V. HABOR,
V. IOVANEL,
V. NESTIANU,
V. NEŞTIANU,
V. PADUREANU,
V. STEFAN,
V.CIUBOTARU,
V.KOBETS,
V.MINCIUNA,
Valerica tudorica,
VALERIU NEŞTIANU,
VARTICIAN ANA,VASILE STEFAN,
VATA LUMINITA GINA,
VEGHE EVA-MARIA.,
VERDES DOINA,VERONICA LUCA,VERONICA SFREDEL,
VICTOR NIMIGEAN,
VICTORIA BELU,
VIORELA CristEa,
VIORELA ENACHESCU,VIRGIL PAUNESCU,
56

9
47
22
44
41
42
34
26
39
12, 30
34
6
14
6
6
38
29
46
30
11

VLAD FLORIN
IOVĂNESCU,VLASE LAURIAN,VOICU TUDORACHE,VORONEANU L,
W.R. SCHÄBITZ,ZGABARUS MIHAELASIMONA,ZOTA IE.,ZOTA LARISA,

13
35
38
23
3
49
49
37, 44

46
49
42
33
22
6
5
38
42, 43
43
40
40
40
30
23
19
43
15, 21, 27
13
21
23
8, 17, 28, 41, 42
4
5
13
17, 4233
Fiziologia ‑ Physiology  2009 Supplement