Prime 95B 25mm™ Scientific CMOS Camera Datasheet
CMOS, EMCCD AND CCD CAMERAS FOR LIFE SCIENCES

95B 25mm
Large Field of View BSI Scientific CMOS
Discovery depends on every photon

25mm

D1608

iagonalThe Prime 95B 25mm delivers an extremely large imaging area andcombines this with the near-perfect sensitivity of a Backside Illuminated(BSI) Scientific CMOS sensor. The Prime 95B 25mm sensor converts
up to 95% of incident photons into measurable signal, and provides
an unmatched 25mm field of view. The Prime 95B 25mm is optimallypositioned to maximize detection and imaging throughput when
mounted on the Nikon ECLIPSE Ti2 microscope.
The extreme sensitivity not only allows fainter signals to be detected,
it provides the flexibility to increase frame rates, or turn down theexcitation intensity to reduce cellular photo-damage. The Prime 95B
25mm improves the field of view and maintains the high frame rates
and extremely low read noise that has made sCMOS so popular forlive-cell imaging.
95% Quantum Efficiency

1608

25mm Field of View (1608 x 1608)
1.6e- Read Noise (median)
30fps @ 16-bit

Features

Advantages

High Quantum Efficiency
95% Peak QE

Maximizes ability to detect weak signals, enables short exposure times for high frame rates, minimizes phototoxicity
across a wide range of wavelengths

Large 25mm Field of View

Maximize imaging area and increase throughput

Large 11µm Pixel Size

Maximize light collection while maintaining proper spatial sampling

Extremely Low Read Noise

Maximize your ability to detect faint fluorescence

Fast Frame Rates

Capture highly dynamic events with high temporal resolution

Enhanced Dynamic Range

Measure both bright and dim signal levels within the same image
50,000:1 Dynamic Range (94 dB)

Multiple Expose Out Triggering

Control up to four light sources for multi-wavelength acquisitions

SMART Streaming

Faster acquisition rates with variable exposures, ideal for multi-probed live cell imaging
Compatible with Multiple Expose Out Triggering

Rev A2-06052017©2017 Photometrics. All rights reserved.

1

95% Quantum Efficiency

Prime 95B 25mm™ Scientific CMOS Camera Datasheet

2.6 Megapixel BSI CMOS Sensor
Backside Illuminated Sensor
1.6e- Read Noise (Median)>95% peak QE80,000e- full well
11 x 11μm pixels
25mm diagonal

Easily Mounted and Secured
F-mount
Two ¼”-20 mounting holes per side

Convenient Interface
16-bit Data• 30fps

Multiple Cooling Options
Forced Air Cooling• -10ºC Cooling• Selectable Fan Speed
Liquid Cooling• -25ºC Cooling• Leak-proof, quick-disconnect ports

Advanced Triggering Capabilities
Effective Global Shutter
Up to four selectable expose-out lines

Rev A2-06052017©2017 Photometrics. All rights reserved.

2

Prime 95B 25mm™ Scientific CMOS Camera Datasheet

95% Quantum Efficiency

Specifications

Camera PerformanceSensor

GPixel GSense 400 BSI CMOS Gen IV, Grade 1 in imaging area

Active Array Size

1608 x 1608 pixels (2.58 Megapixel)

Pixel Area

11μm x 11μm (121μm2)

Sensor Area

17.69mm x 17.69mm
25mm diagonal

Peak QE%>95%
1.6e- (Median)

Read Noise

1.8e- (RMS)

Full-Well Capacity

80,000e-

Dynamic Range

50,000:1

Bit Depth

16-bitReadout Mode

Rolling Shutter
Effective Global Shutter

Binning

2x2 (on FPGA)

Cooling Performance

Sensor Temperature

Dark Current

Air Cooled-10ºC @ 30ºC Ambient

2.9e-/pixel/second

Liquid Cooled-25ºC @ 30ºC Ambient

0.7e-/pixel/second

Specifications

Camera Interface

Digital Interface

PCIeLens Interface

F-Mount

Mounting Points

2 x ¼ 20” mounting points per side to prevent rotation

Liquid Cooling

Quick Disconnect Ports

Triggering Mode

Function

Input Trigger Modes

Trigger-First – Sequence triggered on first rising edge
Edge – Each frame triggered on rising edge
SMART Streaming – Fast iteration through multiple exposure times

Output Trigger ModesFirst Row – Expose signal is high while first row is acquiring data
Any Row – Expose signal is high while any row is acquiring data
All Rows – Effective Global Shutter – Expose signal is high when all rows are acquiring data

Output Trigger Signals

Rev A2-06052017©2017 Photometrics. All rights reserved.

Expose Out (up to four signals), Read Out, Trigger Ready

Note: Specifications are subject to change.

3

Prime 95B 25mm™ Scientific CMOS Camera Datasheet

95% Quantum Efficiency

100
Frame Rate (PCIe interface)

QUANTUM EFFICIENCY (%)

9080
7060
5040

Array Size

16-bit1608 x 1608

301608 x 1200

411608 x 512

961608 x 256

1921608 x 128

384

3020100400500600700
WAVELENGTH (NM)

800

900

1000

Accessories (Included)

Accessories (Additional)

PCIe Card/Cable

Manuals and QuickStart Guide

Liquid Circulator

Trigger Cable

Performance and Gain Calibration Test Data
47.13 1.855
DISTANCE FROM F-MOUNTFLANGE TO SENSOR

Liquid Cooling Tubes

Power Supply

12.16 .479

162.23 6.387

27.22 1.071

8X 1/4-20 UNC

6.35 0.250
12.16 .479

8X 1/4-20 UNC

6.35 0.250

162.23 6.387

146.70 5.776
201.61 7.937

108.39 4.267

47.13 1.855
DISTANCE FROM F-MOUNTFLANGE TO SENSOR
59.75 27.22
2.352 1.07134.89 1.374
59.75 2.352

LEFT

108.39 4.267

108.39 4.267

300

12.16

FRONT

146.70 5.776

34.89 1.374

108.39 4.267

8X 1/4-20 UNC

201.61 7.937

LEFT

Photometrics is a registered trademark.
Prime 95B 25mm is a trademark of Photometrics.
All other brand and product names are the trademarks of their respective owners.
Specifications in this datasheet are subject to change.
Refer to the Photometrics website for most current specifications.

Rev A2-06052017©2017 Photometrics. All rights reserved.

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