Official Journal of the Romanian Society of Physiological Sciences

CHIEF EDITOR
CO-CHIEF EDITORSFRANCISC SCHNEIDERIOANA SISKACARMEN TATUASSOCIATE EDITORSMIHAI NECHIFORSORIN RIGAEXECUTIVE EDITORSFLORINA bojin
GABRIELA TANASIE
daciana nistor
	cALIN MUNTEANEDITORIAL BOARD
ARDELEAN AURELBADIU GHEORGHEBĂDĂRĂU ANCA
BENEDEK GYÖRGYBENGA GHEORGHEBUNU CARMEN
COJOCARU MANOLECUPARENCU BARBUCONSTANTIN NICOLAE
HAULICĂ ION
IANCĂU MARIAMIHALAŞ GEORGETA
MUNTEAN DANINAMUREŞAN ADRIANANESTIANU VALERIUOPREA TUDOR(Arad)(Constanţa)(Bucureşti)(Szeged)(Cluj)(Timişoara)(Bucureşti)(Oradea)(Bucureşti)(Iaşi)(Craiova)(Timişoara)(Timişoara)(Cluj)(Craiova)(New Mexico)PĂUNESCU VIRGILPETROIU ANA
POPESCU LAURENŢIU
RÁCZ OLIVERRIGA DANSABĂU MARIUS
SIMIONESCU MAIA
SIMON ZENOSAULEA I. AURELSWYNGHEDAUW BERNARDTANGUAY M. ROBERTTATU FABIAN ROMULUS
VLAD AURELIANVOICU VICTORZĂGREAN LEON

(Timişoara)(Timişoara)(Bucureşti)(Košice)(Bucureşti)(Tg. Mureş)(Bucureşti)(Timişoara)(Chişinău)(Paris)(Canada)(Timişoara)(Timişoara)(Bucureşti)(Bucureşti)Accredited by CNCSIS - B category - code 240
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Fiziologia ‑ Physiology  2010 supplement

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Official Journal of the Romanian Society of Physiological Sciences

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Fiziologia ‑ Physiology  2010 supplement

SYMPOSIUM PROGRAMEUROPEAN STRATEGY FOR PHD PROGRAMSUNIVERSITY OF MEDICINE AND PHARMACY VICTOR BABES TIMISOARA,TIMISOARA,
20-22 SEPTEMBERSYMPOSIUM
PROGRAM 2010
EUROPEAN STRATEGY FOR PHD PROGRAMSUNIVERSITYOF
MEDICINE AND PHARMACY VICTOR BABES TIMISOARA,
Key speakers from EU partner universities:
TIMISOARA,Frankfurt
20-22 SEPTEMBER 2010
• Johann Wolfgang Goethe University- Prof. Josef Pfeilschifter- fromProf.
Key speakers
EUErhard
partner Seifried
universities:
 Johann
Wolfgang
GoetheRadeke
University Frankfurt - proposed dates: 20-21/22.09- Prof.
Heinfried
Prof.
Josef
Pfeilschifter- Prof. Reinhard Henschler- Prof. Erhard Seifried• Szeged- University
Prof. Heinfried Radeke- - Prof.
Prof. Ferenc
ReinhardBari
Henschler

SzegedUniversity
proposed• Medical University of Viennadates: 20-22.09
Prof. Ferenc Bari
- - Prof.
Rudolf Mallinger
 Medical University of Vienna - proposed dates: 21-22.09 - departure to Cluj on 23- - Prof.
Boehm
Prof. Stefan
Rudolf Mallinger-

Prof. Stefan Boehm

Monday 20.09.2010, SENATE HALL – Main University building, 2A Eftimie Murgu Square
Monday 20.09.2010, SENATE HALL – Main University building, 2A Eftimie Murgu Square
14:00-14:10

Marius Raica: Welcome address

14:10-14:30

Simona Dragan: Short presentation of project POSDRU 63117, project partners and roles

14:30-15:00
14:30-14:45
14:45-15:00

Doctoral Schools of EU partner universities - organization, function, stake holders
Josef Pfeilschifter: Frankfurt experience in developing PhD programs
Ferenc Bari: Szeged experience in developing PhD programs

15:00-15:30

Romanian Doctoral Schools - organization, function, stake holders from Romanian partner universities
Carmen Bunu, Pompilia Dehelean: Medical PhD School in Timisoara – past and present
Smaranda Iancu: Medical PhD School in Iasi

15:00-15:15
15:15-15:30

16:00-17:00
16:00-16:20
16:20-16:40
16:40-17:00

Coffee breakPress Conference - Official launch of the project POSDRU/88/1.5/S/63117
EU perspectives of doctoral studies Joint doctoral programs
Erhard Seifried: Frankfurt-Timisoara ERASMUS Cooperation
Ferenc Bari: Cross border HU-RO projects
Corina Duncescu: Orpheus document

19:30-21:00

Dinner

15:30-16:00

Tuesday, 21.09.2010 SENATE HALL – Main University building, 2A Eftimie Murgu Square
10:00-11:00
10:00-10:15
10:15-10:30
10:30-10:45
10:45-11:00
11:00-11:30

Bridging science - meeting with the PhD coordinators from Timisoara and Iasi Medical Universities
Iancu Smaranda: Microbiology as model of “interdisciplinary approach”
Danina Muntean: Mitochondrial centrality in cardio-vascular research – a translational approach
Margit Serban: Trends in paediatric research and their impact on doctoral study
Ioan Sporea: How to drive a PhD student?
Coffee break16:00-17:00
16:00-16:20
16:20-16:40
16:40-17:00

Press Conference - Official launch of the project POSDRU/88/1.5/S/63117
EU perspectives of doctoral studies Joint doctoral programs
Erhard Seifried: Frankfurt-Timisoara ERASMUS Cooperation
Ferenc Bari: Cross border HU-RO projects
Corina Duncescu: Orpheus document

19:30-21:00

DinnerTuesday,
SquareTuesday,21.09.2010
21.09.2010SENATESENATEHALL
HALL––Main
MainUniversity
Universitybuilding,building, 2A
2A Eftimie
Eftimie Murgu Square
10:00-11:00
10:00-10:15
10:15-10:30
10:30-10:45
10:45-11:00
11:00-11:30
11:30- 13:00
13:00-14:00

Bridging science - meeting with the PhD coordinators from Timisoara and Iasi Medical Universities
Iancu Smaranda: Microbiology as model of “interdisciplinary approach”
Danina Muntean: Mitochondrial centrality in cardio-vascular research – a translational approach
Margit Serban: Trends in paediatric research and their impact on doctoral study
Ioan Sporea: How to drive a PhD student?
Coffee break
Poster session of PhD students: Prerequisites for science
Lunch break

14:00-16:00
14:00-14:30

Interdisciplinary approach to medical research
Reinhard Henschler: Experimental Models to study interaction of cellular therapeutics with the blood vessel
wall
Wednesday, 22.09.2010 SENATE HALL – Main University building, 2A Eftimie Murgu Square
14:30-15:00
Dan Gaita: University of Medicine and Pharmacy “Victor Babes” Timisoara – 10 centers for one goal:
15:00-15:30
Excellency research
14:00-16:00 Heinfried
Interdisciplinary
approach to medical
research
15:30-16:00
Radeke: Excellenceclusters:
Building a brand and optimising inter-faculty programs to
14:00-14:30

Henschler:
Experimental Models to study interaction of cellular therapeutics with the blood vessel
attractReinhard
excellent
PhD students

Anca wall
Miron: Bridging ethnobotany and modern medicine
14:30-15:00
Dan Gaita: University of Medicine and Pharmacy “Victor Babes” Timisoara – 10 centers for one goal:
16:00-16:30
Coffeebreak research
15:00-15:30
Excellency
16:30-18:00
Round
table Vienna,Frankfurt,
Biotechnology and Immunophysiology Center,
15:30-16:00
Heinfried
Radeke:ExcellenceSzeged
clusters
Best practiceexchanges
between Doctoral
Anca Miron:
Bridging ethnobotany
and modern medicine Emergency County Hospital Timisoara – Agilrom
Scientific Workshop
16:00-16:30 Schools.
Coffee break
16:30-18:00
Round table Vienna, Frankfurt, Szeged
Biotechnology
and Immunophysiology
Center,Hands-on training– Lab-on-a-chip
technology –Best practice exchanges between Doctoral
Emergency
County Hospital
Timisoara– Agilrom
2100 Bioanalyzer
Agilent– Symposiumfor PhD
Schools.
Scientific
Workshop
students,
coordinated by Florina Bojin
Hands-on training – Lab-on-a-chip technology –19:00
Dinner2100 Bioanalyzer Agilent – Symposium for PhD
students, coordinated by Florina Bojin
19:00
DinnerWednesday, 22.09.2010 SENATE HALL – Main University building, 2A Eftimie Murgu Square
Wednesday, 22.09.2010 SENATE HALL – Main University building, 2A Eftimie Murgu Square
09:00-11:00
Opportunities within European graduate schools
09:00-11:00

Opportunities within European graduate schools

09:00-09:30
Rudolf Mallinger: Doctoral Schools: The European Approach
09:30-10:00
Boehm:The impactof Schools:
Excellence
Programmeof Doctoral Schools at the Medical University of Vienna
09:00-09:30 StefanRudolf
Mallinger:
Doctoral
The European
Approach
Bari:Boehm:
The benefitsof highquality Doctoral
Schoolfor developing
career offorVienna
PhD students
09:30-10:00 Ferenc
StefanThe impact
of Excellence
Programmeof Doctoral
Schools atthe
the research
Medical University
FerencBari: The benefits of high quality Doctoral School for developing the research career for PhD students
10:00-10:30
at Szeged
University
10:00-10:30 Simonaat Szeged
University
Dragan:Perspectives of Doctoral programs at UMFVB
SimonaDragan:Perspectives of Doctoral programs at UMFVB
10:30-11:00
10:30-11:00
Concluding
remarks for best practice exchanges between Doctoral Schools
Concluding remarks for best practice exchanges between Doctoral Schools
11:00-12:00
Pius
Brinzeu
CentreofofResearchresearchpotential
11:00-12:00 Visit to
Visit
to Pius
Brinzeu
CentreResearch –– evaluation
evaluation ofof
thetheresearchpotential
Mihai Mihai
IonacIonac
13:00-14:30
13:00-14:30 LunchLunch

Organizing Committee
Committee
Organizing
Marius Raica
Marius Raica
Pompilia Dehelean
Pompilia
SimonaDehelean
DraganSimonaCarmenBunuDragan
Panaitescu
CarmenAdina
BunuBucur
Panaitescu
CarmenBucur
Tatu
Adina
Florina
Bojin
Carmen Tatu
Florina Bojin
Contact person: Adina Bucur, e-mail: adina.bucur@gmail.com

Contact person: Adina Bucur, e-mail: adina.bucur@gmail.com

Official Journal of the Romanian Society of Physiological Sciences

1. RADIOPACITY OF FIBER POSTS
B. Baldea1, G. Furtos2, D. Bratu1
Faculty of Dental Medicine, Timisoara, Romania”Raluca Ripan” Institute of Research in Chemistry, Cluj-Napoca,
Romania.
1

2

Objectives: The aim of this study was to analyse the radiopacityof some glass/carbon fibers and metal post and to compare withthe radiopacity of human enamel and dentin.
Materials and Methods: Four disks of each post (21 materials),mesiodistally sections of human molar (1±0.01 mm thickness)
and aluminium step wedges were radiographed on dental X-ray
films. After development, dental films were digitized by scan andradiopacity values were recorded for each sample. The radiopacityof the samples was expressed in terms of the equivalent thicknessof aluminium per 1mm unit thickness of material.
Results: ParaPost XP (Coltene Whaledent), FRC Postec Plus
(Ivoclar Vivadent), Danville Ice Light (Danvile), Light Post, DT Light
Post (RTD), showed radiopacity values higher than enamel, Glassix(NORDIN S.A), UniCore Post (Ultradent), Danville Ice Post (Danvile),
ParaPost Fiber Lux, ParaPost TaperLux (Coltene Whaledent) showedradiopacity values significantly greater than dentin while ParaPost
FiberWhite (Coltene Whaledent), RelyX™ Fiber Post (3M ESPE),
Mirafit White, Mirafit Carbon (Hager & Werken), Fibrapost (PDSA),
Saremco posts Non-Stop Fibre (Saremco Dental AG), Aestheti-Plus,
DT White Post (RTD), materials showed radiopacity values lower
than dentin. Composites from Reforpost Glass Fiber (Angelus),
Core post - Glass fiber post, Core post - Carbon fiber post (DenMat)
had radiopacity lower than dentin while the second componentof these posts metal had greater radiopacity than enamel. The
results recorded showed statistically significantly differences(significance level = 0.05) when evaluated with One-Way ANOVA
statistical analysis.
Conclusions: Future fiber posts are recommended to havehigher radiopacity values than dentin and perhaps ideallysimilar to or higher than that of enamel for improved of clinicaldetection. The posts with a lower radiopacity than 1 mm Al
could be considered sufficiently radiopaque if the posts would
be cemented with higher radiopaque cement. Further works inthis direction are needed.

2. PARACLINICAL DIAGNOSTIC VALUE FOR
ENDOCARDITIS INVOLVING OF SEPSIS
Begezsán (Loghin), I. I. , Dorobăţ, M. C.
University of Medicine and Pharmacy “Gr.T.Popa”, Faculty of
Medicine, Department of Infectious Diseases, Iasi, Romania,
Infectious endocarditis is an infection of the endocardiumwith microorganisms present in lesions, indicating that it may
be valvular endocardium, septal defects or persistent arterial
channel. In terms of infection disease, we can say about thispathology that it’s a particular form of systemic infection withunlimited evolution, where the endocardium is the primary focus
of multiplying germs.
Although in the last half century occured major changes
regarding endocarditis, by modifying etiological spectrum,
appearance of new antibiotics and new laboratory technique; also

Fiziologia ‑ Physiology  2010 supplement

the susceptible population has changed, and the proportion of
immunocompromised persons and those with prosthetic valve or
pecemaker increasing at the detriment of those with rheumatic
valvular disease. Despite major advances in diagnosis and treatment,
infective endocarditis remains a disease with high morbidity, andwith a mortality of 20% to 30%. One cause of the high mortalityof infective endocarditis is the long latency from the onset ofsymptoms to the definitive diagnosis of infectious endocarditis
and the initiation of appropriate treatment.
This research project aims main objective to improve theparaclinical methods of investigation and highlighting new usefulparameters, in order to establish the right diagnosis, complete
and early of infectious endocarditis, and also to establish thetreatment at the right time.
Project will be based on two-stage study:
I.Retrospective study targeting diagnosed patients withendocarditis in sepsis by classical procedure and an other groupof suspected patients.
II.Clinical and laboratory correlations between the two groups
which will be added to another group of prospective study (which
could lead in the second stage to conclusions regarding a rightand quick diagnosis).

3. CLINICAL APPLICATIONS OF THE BIOMOLECULARINVESTIGATIONS, PERSONAL CONTRUBUTIONSIN ONCOLOGY
Boldeanu Florina Maria1, Serban Margit2
Regional Center for Transplant Immunology, Emergency
Clinical Country Hospital, Timisoara, Romania
2
University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Romania1

The aim of this project is to investigate residual minimal disease
in oncology utilizing the relationship between fusion genes andoncological diseases, especially residual minimal disease in caseof bone marrow transplantation, and the incidence of these fusion
genes and their identification by molecular biology methods. Afterthe biological samples collection, will establish the group of thepatients for the study, will make hematological and biochemical
analysis, thus will establish the general physical condition before
and after the surgical intervention. Will perform complex molecularbiology analysis to the group of patients in order to investigate
the residual minimal disease
The methods used will be Southern blot analysis, PCR(polymerase chain reaction) analysis of the T receptor cellular
genes, RT-PCR analysis or immunological methods (flowcytometry – is based on detection of the anormal or unusual
phenotype). We will use DNA or cDNA obtained from RNA after
RT-PCR (reverse transcription of the polymerase chain reaction).
Will extract DNA or RNA from the biological samples (peripheral
blood is collected on EDTA) with extraction kits (e.g. Qiagen kit)
or automatic method with MagNa Pure LC Roche. The DNA or
RNA will be amplified by PCR.
The results will be correlated with chronic pathology clinicaldiagnosed, will establish the incidence of these fusion genes andresidual minimal disease. The obtained results will be compared to
the literature data. These results will contain also positive controls
in order to eliminate the false positive results.

5Official Journal of the Romanian Society of Physiological Sciences

4. THE VALUE OF ACOUSTIC RADIATION FORCEIMPULSE ELASTOGRAPHY (ARFI) FOR THE
EVALUATION OF CHRONIC HEPATOPATHIES
Simona Bota
“Victor Babes” University of Medicine and Pharmacy,
Timisoara, Romania, Department of Gastroenterology andHepatology
ARFI is a new method used for the assessment of liver fibrosis,of thyroid gland and breast nodules, provided by Siemens andintegrated into ACUSON S2000 ultrasound system. Unlike Transient
Elastography (TE) – FibroScan, this new technique can be also
performed in patients with ascites.
This research project will assess the liver stiffness (LS) in: 200
patients with chronic HCV hepatitis, 150 with HBV chronic hepatitis,
350 with cirrhosis, 100 subjects without liver disease and 200with malignant and benign liver tumors. In patients with chronic
hepatitis, liver biopsy (LB), TE and ARFI will be performed in thesame session. In subjects without liver disease, LS will be assessedby TE and ARFI. The result of LS measurements by TE and ARFI will
be calculated as the median value of 10 valid measurements.
In patients with liver cirrhosis LS will be determined by meansof ARFI and TE and gastroscopy will be performed. Characterizationof liver tumors will be based on histological examination, imaging
techniques (CT, MRI and CEUS), tumor markers and LS by meansof ARFI, which will be evaluated as compared to those in healthy
liver tissue.
We will compare data from patients with chronic HBV and HCV
hepatitis with LB and we will create a score for predicting liver
fibrosis based on the noninvasive tests. In liver tumors we will try
to predict the nature of tumor based on the ARFI measurements. In
cirrhotic patients we will try to determine if the ARFI measurements
can predict vascular decompensation, esophageal varices andvariceal bleeding.

5. FETAL DISTRESS RELATED WITH PATHOLOGY
OF UMBILICAL CORD AND THE PLACENTA
Brisan, C.1, Brisan, L.2, Bernad, E.1, Meszaros, G. 3,
Munteanu, I.1
Department of Obstetrics and Gynecology University of
Medicine and Pharmacy “Victor Babes” Timisoara
2
Department of General Surgery University of Medicine andPharmacy “Victor Babes” Timisoara
3
University of Szeged, Faculty of Medicine

1

The prenatal diagnosis of fetal distress is an important concernof the current obstetrics. The pathology of the umbilical cord andplacenta is present in a significant number of cases with fetal distress.
Therefore, this project aims to generate the following:- Making a fundamental research related to investigating andanalyzing the characteristics of the flow through the placental unit,
giving a special attention to identifying specific phenomena thattake place at this level in physiological conditions and in various
pathological conditions.
- The study and description of flow phenomena in the fetoplacental circulatory system, in view of identifying key issues of6

the existence of weak flow underlying the production of severe
pathologies associated with fetal development.
More accurate description of the phenomenon of flow inhuman circulatory system shows a great impact both at scientificand social level with long-term benefits for the society.
The novelty is brought by measurements and simulations
conducted to help understand and prevent pathological changesof the feto-placental vascular system highlighting the best time to
extract the fetus from the uterus before the onset of fetal distresswith with psychomotor echo on neural development.
Modern techniques of three-dimensional reconstruction of
the placental circulatory system geometries, numerical simulation
techniques to real phenomenon dimensional flow, measurements
in vivo, is a rather specific means of achieving this project.
We follow these medical and scientific objectives:- identifying the flow issues at umbilical cord blood- highlighting the critical flow, blood flow analysis by noninvasive methods (Doppler cross); experimental flow measurements
and views on real 3D models, correlation and quantification of
results obtained by numerical simulation of blood flow to thecases investigated- combination field parameters for hemodynamic circulation
with placental pathological factors that determine the onset ofpathological blood flow.

6. CLINICAL AND EXPERIMENTAL EVALUATIONOF SOME ALL-CERAMIC FIXED PARTIAL DENTURES
Candea, A. 1, Bratu, D. 1
Department of Prosthodontics, University of Medicine andPharmacy “Victor Babes“, Timisoara, Romania
The aim of my PhD thesis is to study all-ceramic inlay-retained
fixed partial dentures (IRFPDs), because the field is a relatively
new one; they also represent an aesthetic, minimally invasive
alternative to traditional fixed partial dentures using full crowns
as retainers.
My first objective is to study all-ceramic inlay-retained fixed
partial dentures using finite element analysis, in order to assess
geometrically the most favourable design of inlay preparations
which would have the best results in the case of replacing a
posterior missing tooth using all-ceramic IRFPDs.
Another purpose is to carry out a comparative study on thefracture strength of all-ceramic three unit IRFPDs with yttria
stabilized polycrystalline tetragonal zirconia framework andall-ceramic three unit IRFPDs made from a heat-pressed lithiumdisilicate based glass-ceramic.
I also aim at conducting a study on the marginal fit of allceramic three unit IRFPDs.
I want to carry out a clinical study, which would enable
me to evaluate the clinical performance of all-ceramic IRFPDs,
especially the longevity of the restorations and the viability ofthis treatment method.
Should they be favourable, the results of the studies conductedwithin my PhD project could provide a conceptual and practicalframework for the adoption of this minimally invasive method of
replacing a posterior missing tooth, maximizing the preservationof dental tissue and diminishing the risks which might occur

Fiziologia ‑ Physiology  2010 supplement

Official Journal of the Romanian Society of Physiological Sciences
during the tooth preparation for full-crowns. We hope to collectvaluable data which will throw new light on the suitability of thistype of treatment.
Keywords: all-ceramic, minimally invasive, metal-freeprosthodontics, inlay-retained fixed partial denture, marginal fit

7. Body weight loss using Meal Replacements
Chirila, I.
PhD student at ”Gr. T Popa” University of Medicine andPharmacy Iasi
National Institute of Public Health - RCoPH Iasi
Introduction. Given the increasing incidence of overweight
and therefore escalating associated health care costs, healthcare
providers must discover how to effectively treat this complex
condition. A topic discussion in international literature is use ofmeal replacements (MR) to help patients prevent weight gain,
optimizing individual interventions for weight reduction and longterm maintenance. Study objective was to assessing effectivenessof MR in body weight management, for people of working age.
Material and methods. Overweight or obese adults followed
a low-calorie diet using MR, at least six weeks and indicators were
followed: size, weight, percentage of fat (bioimpedance) and bodyperimeters. Were proposed a customized diet, replacing two
meals a day with MR formulas and the third meal was composedof conventional, balanced food. This strategy generally provided
5040 - 6700 kJ (1200 -1600 kcal) per day and slightly high protein(1.1 to 1.3 g / kg, 20-35% of energy), low lipid (15-25%) and low
carbohydrate (35-60%).
Results and discussion. A group of 44 adults (age 42.91 ±14.5 years , BMI = 34.3 ± 5.7 kg/m2; 32 female with 92.3 ± 14.1 kg,
fat = 44.9 ± 7.3%, waist 115.7 ± 12.7 cm, hip 120.2 ± 10.6 cm and12 male with 107.4 ± 23.8 kg , fat = 34.1 ± 6.5 %, waist 117.3 ±18.2 cm, hip 114.7 ± 9.0 cm) followed a customized weight- loss
program for average 4.02 months (± 0.33 SE) and the entire grouplost on average 11.87 kg (1.06 ± ES) of body weight, 13.67 cm (±1.58 SE) in abdominal perimeter and 5.85 (± 0.63 SE) in percentage
of body fat.
Conclusions. High protein MR increase satiety and decrease
food intake, being helpful for energy-restricted diets in improving
weight loss with retention of lean mass.

8. A HIGHER SUCCESS RATE OF DENTAL IMPLANTS
THROUGH PROPHYLAXIS
Cristescu Caius
University of Medicine and Pharmacy “Victor Babes” Timisoara
The purpose of the project is to develop a methodology thatsums up specific and innovative prophylactic methods applied
before and after the dental implant, aiming to increase the overall
quality of the patient’s life.
The projects stages will be implemented as follows:
1. Identifying dental practitioners specialized in dental
implants.

Fiziologia ‑ Physiology  2010 supplement

2. Briefing the practitioners about the project, its purpose andtheir involvement in the project
3. Creating the patient database
4. Before the implant:- Informing the patients about oral and dental threatening
substances- Informing the patients about proper dental brushing,
tongue cleaning, oral rinsing and oral showers – practicaldemonstrations- Identifying and visualizing bacterial plaque – practicaldemonstrations- Applying local prophylactic age-specific measures: fluoride
treatment, dental sealing- Professional complex cleaning of the oral cavity and teeth- Recommendations about nutrition and diet
5. Taking part at the surgical procedures preceding thedental implants ( sinus lift, bone graft, additive bone procedures,
repositioning of the mandibular canal, etc - if they are necessary,depending on the clinical situation) and at the surgical insertionof the dental implant.
6. After the implant:- Evaluating the degree of oral hygiene- Oral rinsing and oral showers- Prophylactic measures, age-specific- Complex professional dental cleaning
7. Comparative study of the patients who accepted the prophylactic
measures, as opposed to the patients who refused it- Centralizing the data collected through the customized forms
from the implantologists- Data analysis and interpretation- Conclusion

9. EVALUATION OF CLINICO- FUNCTIONALSTATUS AND LIFE QUALITY IN PATIENTS WITH
AUTOIMMUNE RHEUMATIC DISEASES BASEDON A COMMON FUNCTIONAL INDEX
Razvan Gabriel Dragoi
University of Medicine and Pharmacy “Victor Babes” Timisoara
Timisoara City University and Emergency Hospital – Medical
Rehabilitation and Rheumatology Department
State of the art and potential contributions to its development
The autoimmune inflammatory rheumatic diseases arecaused by autoimmune pathological processes, leading toclinical manifestations and imaging biological specific. In
the current doctoral study I try to find a common way inevaluating the autoimmune rheumatic diseases that result
by joint destructions and specific conditions, lack in the
mioartrokinetic chain(MAK) progressively increased, leading
to decreased quality of life of these patients, including direct
and indirect significant costs.
Project objectives
The research project has as main objective the improvementin the methodology for tracking biological and clinicalprognosis in major autoimmune diseases with osteoarticular7

Official Journal of the Romanian Society of Physiological Sciences
destruction and musculoskeletal injury. The secondary
objectives of the proposed research project are: Quantifying
the contribution that brings quality of life assessment in the
management of patients with autoimmune rheumatic disease.
Optimizing recovery treatment in patients with autoimmune
rheumatic diseases, by setting the timetable therapeuticinterventions based on monitoring of quality of life scores.
Identify whether the initiation of biological therapy, depending
on clinical progress and determining functional prognosis index.
Extension of interdisciplinary cooperation areas (medicine,
physiotherapy) to develop new methodologies and solutions
for improving the efficiency of the treatment in patients with
autoimmune rheumatic diseases.
Keywords: life quality, functional index, Rheumatoid
arthritis, Ankylosing spondylitis, Psoriatic arthritis

10. THE ROLE OF MITOCHONDRIA IN CARDIOPROTECTION OF SENESCENT AND PATHOLOGICALMYOCARDIUM
Duicu, O. M., Muntean, D.
Department of Pathophysiology, “Victor Babes” University of
Medicine and Pharmacy Timisoara
Early myocardial reperfusion, the only effective therapy able
to reduce the infarct size and to improve the clinical outcome,is also responsible for the lethal reperfusion injury that may
paradoxically reduce the beneficial effects of revascularization.
Mitochondria play a central role in initiating cell death inducedby the postischemic reperfusion as well as in cardioprotection
against it. Accordingly, modulation of mitochondrial functionin the settings of ischemia/reperfusion (I/R) injury represents
a major therapeutic target of pharmacological agents able
to reduce the cell death at reperfusion. Several drugs were
demonstrated to reduce lethal reperfusion injury whenadministrated as monotherapy in different experimental
settings. However, multiple pharmacological associations
represent a recently investigated therapeutic alternative
aimed at increasing cardioprotection at reperfusion while
decreasing the doses.
The aim of the present project is to assess the efficiency of
cardioprotective strategies applied during the postischemic
reperfusion in aged animals and with experimentally induced
pathology (diabetes and hypertension).
The objectives of the project are: 1) implementation andvalidation of the methodology for studying permeabilized
myocardial fibers isolated from senescent and pathological
rat hearts subjected to different protocols of global I/R injury,2) assessment the possibility to enhance cardioprotection atreperfusion in these settings by modulating mitochondrial
function via the association of pharmacological agents known
for their individual protective effect and 3) investigation of
the possibility to recapitulate the protective effects in humanpermeabilized fibers harvested from patients undergoingheart surgery.
Keywords: ischemia/reperfusion injury, senescent andpathological heart, cardioprotection, permeabilized fibers
Research supported by the National Authority for Scientific Research
grant 42-122/2008 and Fellowship Project 1.5/88/S/ID 6311.

8

11. THE QUALITY OF THE ENDOMETRIUM IN
INFECTIOUS ETIOLOGY FEMALE UTERINE INFERTILITY.
CORRELATIONS BETWEEN INFLAMMATORYFACTORS AND MICROBIAL AGENTS (Chlamydia
trachomatis, Mycoplasma hominis, Ureaplasma
urealyticum)
Dumitriu (Miron) Nora, Anghelache-Lupascu Ivona
University of Medicine and Farmacy “Gr.T.Popa”, Iasi, Romania
Departament-Obstetrics and Gynecology
Methodology: The research project will be investigated a totalof 200 patients diagnosed with primary or secondary infertility ofuterine question. It will make hysteroscopy which will remove theendometrial biopsy, and after anatomic-pathologic examination will
determine whether inflammatory endometrial pathology is present(endometritis).There will also be collected samples to determine
the presence in the uterine cervix, the following agents: (Chlamydia
trachomatis, Mycoplasma hominis, Ureaplasma Urealyticum). For
microbiological examinations will be used the following methods:• Detection of Mycoplasma hominis and Ureaplasma urealyticum andsensitivity to antibiotics will make galleries using Mycoplasma IST
2 (Bio-Mérieux, France) will be tested for sensitivity to the following
antibiotics: doxycycline, josamycin, ofloxacin, tetracycline, erythromycin,
ciprofloxacin, azithromycin, claritomicine and pristinamycin.
• Chlamydia-antigen detection will be realised with Chlamydia
Microplate EIA - BioRad; also will correlate with the presence orabsence of Ig A, Ig M, Ig G serum Chlamydia from patients with
Nova Lisa Novatec GmbH Immunodiagnostica.
The main objective of the study is diagnosis and treatment ofendometrial pathology of inflammatory and infectious, the causeof female infertility.
The secondary objectives: We will try to determine the presenceof infection correlated with inflammatory markers in serum or at
the endometrial biopsy sample (immunohistochemistry methods
will be used for proper determination of cytokines: IL-1, IL-8, TNFα).
Will compare data from tests conducted on these patients and will
try develop a therapeutic protocol in endometrial inflammation,
the ability to restore fertility to these women.
Keywords: Female uterin infertility, Embryo implatation,
Genital infections, Hysteroscopy, Endometrium derived cytokinesand growth factor.

12. THE INFLUENCE OF AMBULATORY BLOOD
PRESSURE PROFILE AND RISK FACTORS ON
LEFT VENTRICULAR GEOMETRY
Florea Cristina“Victor Babes” University of Medicine and Pharmacy Timisoara
The objective of the study was to determine the influence ofhigh blood pressure values and profile determined by ABPM, onleft ventricular hypertrophy and LV geometry.
Material and methods: Ambulatory blood pressure monitoring
was applied to 65 patients, with age of 50±6 years, with mild toFiziologia ‑ Physiology  2010 supplement

Official Journal of the Romanian Society of Physiological Sciencesmoderate hypertension, sever­ity stages I–II, who had never beentreated and had a standard echocardiographic evaluation and risk
factors evaluation. LV hypertrophy was considered present when
LV mass index (LVMI) was >125 g/m2 in men or 110 g/m2 in women.
Increased relative wall thickness (RWT) was present when the ratio
septal wall thickness + posterior wall thickness/LV inter­nal dimension
in diastole was > 0.43.
Results: With regard to the ambulatory blood pressure profiles,
patients were divided into two groups: group 1, consisting of 36(55.45%) patients, in which night blood pressure levels decreasedby more than 10% compared to their daytime blood pressurelevels (dipper) and group II, with 29 (44.6%) patients, whose bloodpressure levels did not decrease as much (non-dipper). Mass index
and relative wall thickness of the patients established the following
echocardiography data: normal geometry, concentric remodeling,eccentric hypertrophy, and concentric hypertrophy. In the dipper
and non-dipper groups’ age, gender, systolic, and diastolic bloodpressure showed no significant differences. Normal geometry,
concentric remodeling, and concentric hypertrophy were nearly
similar in both groups. In group I, normal LV geometry was present
at 27.7%, concentric remodeling at 30%, eccentric hypertrophy
at 8.3% and concentric remodeling at 33.3%. In group II, normal
geometry was present at 24%, concentric remodeling at 13.7%,
eccentric remodeling at 37.9% and concentric hypertrophy at
24.1%. Eccentric hypertrophy was higher in the non-dipper group,compared to the dipper group (37.9% vs 8.3%, p < 0.05). Risk
factors present at group II were: diabetes at 42%, dyslipidemia
at 45%, smoking at 27%, obesity at 40.5% and multiple risk
factors at 58.5%.
Conclusions: patients with mild and mod­erate hypertension, whosenight blood pressure does not decrease enough, develop eccentric
hypertrophy more often compared with the dipper hypertensive
patients. Risk factors associated with eccentric hypertrophy wereobesity, diabetes and dyslipidemia.
Key words: hypertension, dipper, non-dipper, ambulatory bloodpressure monitoring, left ventricular geometry, risk factors

13. CONTRIBUTIONS TO THE ELUCIDATION OF
TRITERPENIC COMPOUNDS ANTITUMORALEFFECTS
Gheorgheosu D.E.1,2, Dehelean C2., Muntean, D.1
Department of Pathophysiology
Department of Toxicology “Victor Babeş” University of Medicine
and Pharmacy, Timişoara

1

2

Increasing body of evidence suggests that several
molecules derived from natural compounds are promisingtherapeutic agents against carcinogenesis. Among thesetriterpenes (eg., betulinic acid, betulin, lupeol and oleanolicacid) have shown to possess anti-proliferative properties
against multiple tumoral cells lines. In particular, betulinic acidhas been consistently associated with important biologicalantiviral, anti-inflammatory, antiseptic effects, and alsowith the inhibition of cancer cells growth. The antitumoral
property of betulinic acid has been related to its ability to
induce caspase activation in a mitochondria dependent
fashion, as recently demonstrated in several tumor cell lines
by a reduction in mitochondrial membrane potential andFiziologia ‑ Physiology  2010 supplement

cytochrome c release. Recent experimental evidence forother triterpenic compounds relates the antiapoptotic effectto reactive oxygen species overproduction that was directly
responsible for the reduction in the mitochondrial membranepotential as well as mitochondrial dysfunction.
The aim of the present work is to gain further insights into theantitumoral effects in relation with the mitochondrial function/
dysfunction of two natural triterpenic compounds (betulinic acidand betulin) after acute and chronic administration in transgenic
mice C57BL/6 with experimentally induced carcinogenesis andplurivisceral metastasis.
The project objectives are: (i) implementation and validationof an experimental model for the study of liver mitochondria
function after isolation by differential centrifugation technique;(ii) evaluation of acute and chronic administration of twotriterpenoid compounds (betulin and betulinic acid) on liver
mitochondria and (iii) assessment of acute and chronic effectsof triterpenic compounds on liver mitochondrial suspension intransgenic mice with experimentally induced carcinogenesis andliver metastasis.
Keywords: triterpenic compounds, cancer, mitochondria,
apoptosis, cytochrome c
Research partially supported by the Fellowship Project
1.5/88/S/ID 6311.

14. ELECTRONEUROPATOLOGYCAL PATTERNOF EEG BRAIN MAPPING IN HYPERTENSIVEDISEASE ASSOCIATED WITH PREEXISTINGPREGNANCY
C. Ilea, Ivona Anghelache Lupascu
University of Medicine GR. T. POPA, Obstetrics andGynecology Departament
CUZA VODA Hospital, IASI , ROMANIA
The research project will be evaluated in terms ofquantification and localization of brain electrical activity
by topographic EEG mapping a group of patients including
women with hypertensive disease associated with pregnancy-especially without obvious clinical manifestation framedin the category of pregnant women with obstetrical risk.
It will consider highlighting and spatial distribution ofelectroneuropatologycal pattern of brain activity in focal/
or diffuse lesions of hypoxic encephalopathy. In consecutive
maternal monitoring study group will include both a balance
hypertension clinical-HTA curve, weight curve, diuresis,
subjective complaints, and one paraclinical cardiovascular,
kidney, liver and blood chemistries changes. Fetal monitoring
surveys will be performed by repeated clinical obstetrics
investigation, as well as by ultrasound assessment of fetal
wellbeing, including placental maturity degree and Doppler
velocimetry of umbilical or cerebral fetal artery. It will aim toestablish statistical correlation between the anomalies detectedby EEG MAPPING and clinical/paraclinical accusations-with orwithout changes in blood pressure, pathological ultrasoundaspects of umbilical/cerebral artery IR, fetal IUGR without
an identificable cause, and whether these changes can be a
predictive factor for maternal and fetal prognosis
Key words: pregnancy, EEG mapping, hypertensive disease9Official Journal of the Romanian Society of Physiological Sciences

15. EVALUATION OF SUDDEN DEATH RISK IN
ATHLETES, DIAGNOSIS AND ELECTROPHYSIOLOGICALTREATMENT
Negru, A. ², Drăgulescu, St., I. ¹,², Ivănică, G.², Negru, S².,
Ivanica, A².
Instiute of Cardiovascular Diseases Timisoara, Romania
University of Medicine and Pharmacy „Victor Babeş” Timişoara,
Romania

1

2

Background and Purpose: Sudden death in athletes isinsufficiently studied in relation with its gravity, in terms of
subjacent mechanisms, triggering, diagnosis, risk stratification andtreatment of the various underlying arrhythmias. In our previous
experience a significant number of the acquired and congenital
heart diseases, were strongly associated with sudden death risk
(mostly during exercise). We consider that the actual screening
chart for athletes by standard and effort ECG is not enough accurate
to assess those at high risk.
Methods: The present study proposes the inclusion of minimum
80-85 athletes who consent to electrophysiological screening inpresence of any rest or effort ECG changes, any clinical history ofarrhythmia (presence of at least one anamnesis or documented episode),
resuscitated cardiac arrest or syncope, in which the electrophysiologicalstudy will evidence and trigger any form of tachycardia. Depending of
the arrhythmogenic substrate the radiofrequency catheter ablation
will be tempted in selected cases.
Expected results: We estimate the inclusion of 80-85 patientswith proved significant rhythm disorders. A risk chart will be provided
after statistical analysis of the study group, which will consider the
benefits of additional investigations (ECG, echocardiography, andelectrophysiological study), the mechanisms and the complex
management of cases after diagnosis.
Conclusions: The athletes at high risk, malignant arrhythmiasof all types have maximum indication of exploration, guided bya thorough algorithm and adequate treatment.

16. INTEGRATING MEDICAL DATA IN THE
ELECTRONIC HEALTH RECORD
Leonard Mada
“Victor Babes” University of Medicine and Pharmacy
Timisoara
Introduction: Existing Electronic Health Records are largely
confined to a single or a limited number of providers, missing
the numerous interactions between different medical specialties
and health care providers within a modern health care system. In
modern health care, the medical information is actively exchanged
between the three levels of the system, primary health care,specialty outpatient care and inpatient care. Additional data
stemming from peripheral sensors and directly from the patient
will become more prevalent in the future.
Objectives: The primary objective is to develop the principles andmechanisms for automatic semantic and temporal integration of allavailable medical data in a unique electronic health record. Secondary
objectives include the semantic and temporal standardization of
medical data to enable secondary reuse of this data.
Materials and methods: Applying a formal analysis of semantic

10

and spatio-temporal relationships in medical data and generalization
and abstractization of data types and relations. Extending medical
ontologies on the generated model. Defining interactions between
data objects. Developing standardized query mechanisms forsecondary reuse of medical data, including epidemiologic studies,
quality management, automatic evaluation of loco-regional,
temporal, genetic and other risk factors; facilitating clinical trials,
including enhanced electronic recruitment, automatic monitoringof trial subjects and enabling pharmacovigilance studies.

17. LONG TERM PROGNOSIS OF PATIENTSAFTER HEMATOPOIETIC STEM CELLS TRANSPLANTATION
Mirci-Danicar, O.C, Serban M.
“Victor Babes” University of Medicine and Pharmacy, Timisoara,
Romania
Background: Hematopoietic stem cell transplantation(HSCT) is a life saving procedure, for many severe diseases,
without other therapeutic alternatives. In Romania it was
initiated in Bone Marrow (BM) and Hematopoietic Stem Cells(HSC) Transplantation Center of the Children’s Emergency
Hospital “L. Turcanu” Timisoara with autologous transplant (2001),
allogeneic related donor (2003), allogeneic unrelated donor(2009), allogeneic stem cells transplant from umbilical cord (2009).
The purpose of this study is to develop a protocol for screening,monitoring and diagnosis of secondary morbidity of HSCT and
identify the factors that can improve the long-term prognosis of
the patients and increase their quality of life.
Objectives: 1. Secondary pathology detection of HSCT bydynamic analysis of clinical and biological parameters following apredetermined algorithm to clearly identify the factors influencing
long-term prognosis of patients with HSCT. 2. Identifying factorsthat cause secondary pathology of HSCT. 3. Impact on quality oflife of the developed protocol as a result of identifying factorsthat influence secondary pathology of HSCT.
Study Material: The study will be retrospective and prospective:
patients who underwent HSCT since 2001 and patients that will betransplanted until the end of 2012 in BM and HSC Transplantation
Center from our hospital. As part of the study, I will perform adescriptive and predictive experimental, multidisciplinary model,
on Sprague Dawley rats. This should determine whether brain
natriuretic peptide (BNP) has predictive value for veno-occlusive
disease, cardiac dysfunction and major hydroelecrolitic imbalanceson rats after HSCT.
Key words: hematopoietic stem cells transplantation, longterm prognosis, quality of life, brain natriuretic peptide

18. THE INVESTIGATION OF CARBON MONOXIDEINTOXI-CATION IN FORENSIC MEDICINE
Muresan Camelia Oana¹, Enache Alexandra¹, Talos Ioan²,
Sturz Valentima²¹University of Medicine and Pharmacy “Victor Babes”, Timisoara,
Romania²Institute of Legal Medicine, Timisoara, Romania
Carbon monoxide is a colorless and odorless gas generated in the

Fiziologia ‑ Physiology  2010 supplement

Official Journal of the Romanian Society of Physiological Sciences
incomplete combustion of organic substance. It binds by the hemic
protein, including hemoglobin (the affinity is 210 times bigger thenfor the oxygen) and myoglobin, replacing the oxygen and reducing
it’s capacity to release oxygen, witch leads to hypoxia.
The objectives of my research are to implement a techniqueof work to determine the carboxihemoglobin concentration byelectrophoresis method (the determination of carboxihemoglobine
is made, usually, by spectrofotometric method) and is toimplement a technique of work to determine the concentrationof carboximyoglobin (carbon monoxide binds by hemoglobin andmyoglobine). Another objective is to determine the concentrationof hydrocyanic acid and carboxihemoglobin in the blood of thevictims of a fire, to determine if the death occurred after carbon
monoxide poisoning or due to hydrocyanic acid poisoning, or
because of the combination of them. I also want to determine
the lethal concentration of carboxihemoglobin taking into
account various parameters (age, sex, associated diseases, ethanol
intoxication or intoxication with other substances). I want todetermine the concentration of carboxihemoglobin in the blood
kept at different temperatures (blood stored for 15 minutes at
50°C, at 70°C and at 90°C) and see the relationship between agiven storage temperature and the possibility to still determine
the carboxihemoglobin.

19. RESTORING TOTAL EDENTULOUS PATIENTSWITH PROSTHETIC RESTORATIONS ON SPLINTEDIMPLANTS
Naiche D.1, Bratu D.1
Department of Prosthetic Dentistry, University of Medicine
and Pharmacy “Victor Babes “ Timisoara, Romania

1

The aim of my PhD thesis is studying the behavior of different
types of prosthetic restorations (fixed or removable) supported
on splinted implants in totally edentulous patients.
Usually before loading implants need a rest period of 3 or 6months (mandible respectively maxilla), to osteointegrate. Rigid
splinting reduces mechanical stress exercised on each implant,
increases primary stability of the pluri-implant structure, whichallows immediate loading with a provisional restoration.
One of the purposes of my research project is to register tensions
that occur at the interface between Mezostructure (abutments
splinted with a bar) and Superstructure (Prosthesis), when the
Prosthesis is subjected to forces of insertion and desinsertion,
similar to those applied by the patient.
Another goal of the project is to compare how the occlusalpressures are distributed in bone to implant interface ( after
splinting the abutments ), being known that after loading theimplants, around their necks appears a conoidal bone resorption,
especially in the first year of function.
Stress at the bone - implant interface will be registered in two
situations: when implants are splinted with a bar made in dentallaboratory and when implants are splinted by cold welding of
the titanium bar, directly intraoral. The process of cold welding
is called syncrystallization, and the device used for this purpose
is called SINCRISTALLIZZATRICE . This method could simplify andshorten implant prosthetic treatment, the stage for manufacturing
the bar in dental laboratory, no longer being needed.
The results obtained from recordings made with these studies

Fiziologia ‑ Physiology  2010 supplement

will allow conclusions to be drawn about how effective is splinting
implants in supporting prosthetic restorations.

20. IMPROVING MATERNAL RISK PREDICTION
IN PATIENTS WITH HYPERTENSIVE DISORDERS
OF PREGNANCY BY ASSESSING THE ROLE
OF CLASSICAL AND NOVEL ULTRASOUNDTECHNIQUES IN DIAGNOSTIC AND PROGNOSTIC
PROTOCOLS
Dan Pascut, Rodica Avram
University of Medicine and Pharmacy “Victor Babes” Timisoara
Hypertensive disorders represent the first cause of medical
complications during pregnancy. Our aim is to optimize theprotocol for imaging diagnosis and prognosis in hypertensive
disorders of pregnancy (HDP) by assessing the utility of Tissue
Doppler echocardiography (TDE), which would considerably
improve the outcome of these patients (p) both during pregnancy
and postpartum.
This study will be conducted in close collaboration with colleagues
at the Clinical Hospital of Obstetrics and Gynecology Dr. Dumitru
Popescu Timisoara (CHOGDPT) over a period of 3 years. We expect
to include at least 300 p. Hemodynamic and clinical parameters,
lab tests, ECG, Doppler imaging of peripheral circulation anduterine artery, classical trans-thoracic echocardiography and TDE
will be recorded, assessing the role of EDT in predicting the risk ofmortality and morbidity in HDP. Various parameters (body massindex, excess weight during pregnancy, ultrasound parameters)
will be assessed as potential risk predictors for the occurrence ofcomplications.
The lot will be classified in p with chronic hypertension,
gestational hypertension, early preeclampsia (<34 weeks gestation),late preeclampsia (> 34 weeks) and preeclampsia superimposed on
chronic hypertension. P will be followed until the end of pregnancy
and at 1 and 6 weeks postpartum according to CHOGDPT andpresent study protocols. Using this multidisciplinary approach,
we also aim to find eventual correlations between utero-placental,
fetal and maternal circulation.

21. INTERFACE STUDIES OF PARTIAL FIXEDPROSTHESIS
Emanuela Petrescu, Mihai Rominu
UMFT “Victor Babes”, Romania, Timisoara, Faculty of Dentistry,
Department of Propedeutics and Dental Materials
This research project’s objectives are the improving of
quality and adhesion of partial fixed prostheses interfaces
made from different materials. These objectives are: testing
the physical parameters of prostheses, mechanical resistance,
share bond strength, through invasive and non-invasive
techniques. Investigation methods involved are PC software
numerical simulations, imagistic investigations involving
optical microscopy, optical coherent tomography in Time
Domain mode, Spectral Domain, Micro Computer Tomography,
Electronic Microscopy. Partial fixed prostheses can be made11

Official Journal of the Romanian Society of Physiological Sciencesfrom different materials which can assure a different qualityof prosthesis. The interfaces between materials involved inperforming prostheses, are metal-polymeric, metal-ceramic andall ceramic. Probes are divided depending on the material fromwhich they are made, the conditioning and testing protocol,
are proposed for this project.
The special part of this paper intends to investigate theinterfaces through non-invasive technologies as Optical Coherent
Tomography, Micro-computer Tomography, Optic and Electronic
Microscopy and chemical analysis. These investigation methods
will test metal-ceramic and all ceramic partial fixed prostheses
which were conditioned through different methods for improving
adhesion.
The researches will target conclusions with clinicalapplicability and will try to obtain positive long term effects
through bracing the quality and thorough research into thementioned domain.

22. MODERN THERAPEUTIC APPROACHES IN
ARTERIAL OCCLUSIVE DISEASES OF LOWER
LIMBS
Popa, S.1, Datcu, D. M.1,2
University of Medicine and Pharmacy “Gr. T. Popa”, Iasi,
Romania2“Sf. Spiridon” Hospital, Ist Medical clinic, Iasi, Romania

1

Background:
Transplantation of various bone marrow derived STEM cells leadto engraftment of the cells in the transplanted area, with provenevidence that indicates the possibility of generating specialized
cells according to the area needs, contributing to regenerationof tissues, organs etc
Objectives:
The goal of the study is to induce angiogenesis in affected lower
limbs, aiming to modify the following medical conditions: reduce
the need of limb amputation, increase vascular perfusion index,
reduce distal trophic lesion, increase the distance of independent
walk, as well as pain reducing.
The overall output aims to increase the quality of life of affectedpatients and to offer valid alternative to surgical treatment. Thesemethods would give a long-term positive effect for these potentiallyinvalidating diseases.
Methods:
The study protocol will follow a classical clinical approach. We
will start with the selection of subjects, subsequently dividingthem randomly into two (case and control) groups. Evaluationof the detailed individual state following well establishedparameterization. The procedure consists of bone marrowfrom iliac crest extraction and immunophenotyping, followed
by intramuscular injection of the aspirate near vascular lesion.
After the procedure both groups will be monitorized for healing
process evolution.
Expected results:
Identification of an efficient treatment for PAOD and the typeof adult STEM cells most potent in angiogenesis.
Keywords: Peripheral Arterial Occlusive Diseases (PAOD),
neovascularization, adult stem sells, autologus bone marrowtransplant

12

23. CLINICAL ASPECTS AND OUTCOME OF DEPRESSIVE EPISODES IN RECURRENT DEPRESSIVEDISORDER VERSUS DEPRESSIVE EPISODES INBIPOLAR DISORDERRomoşan, R.
“Victor Babeş” University of Medicine and Pharmacy,Timişoara, Romania
The general objective of this study is to identify potential
differences in clinical symptoms and outcome between depressive
episodes in recurrent depressive disorder and depressive episodes
in bipolar disorder.
The research results will enhance specific knowledge regarding
the depressive episode in bipolar and recurrent depressive disorder,
favoring the technological transfer in the diagnostic domain(clinical and standardized), the drug therapy domain (benefit
which could be taken into consideration by the pharmaceuticalindustry) and the non-drug therapy domain (benefits that can betaken into account by the psycho-social practices).
Another aim of this study is to disseminate the obtained
information by publishing it in articles, journals and presentationsat specific scientific events (both national and international)
which can lead to new collaboration possibilities in the specificresearch field.
Research results are expected to be significant, both clinicalas well as the neurocognitive, retrieval of profiles of depressive
episodes may lead to both a better understanding of the disorderand the correct diagnosis of the first episodes of disease andtherefore optimize therapeutic strategies to enable a goodremission and stability over time and thus improve the qualityof life of the patients.

24. Qualitative and Quantitative Analysis of
Bacterial Aerosols in Dental Practice, indoor
air quality improvement alternatives
Serban, D., Vlaicu, B.
”Victor Babes” University of Medicine and Pharmacy Timisoara,
Romania
The spread of infection has long been considered one of
the main concerns in the dental community. Indeed, infectious
agents may be transmitted to patients and dental staff via
several vectors, including instruments and air. Bioaerosols are an
important consideration for infection control and occupational
health, as these particles may consist of or convey potentiallyhazardous microorganisms, allergens, or other toxic substances.
Infective causative agents may include bacteria, viruses, fungi,
and possibly even prions.
Although the existence of dental and microbiologic aerosols
has been known for a long time, the scientific analysis of therole they have in dentistry has been investigated only recently.
Furthermore, data concerning bacterial contamination of air in
multichair dental clinics, such as those found in dental schools,
hardly exists.
The main objective of this study is to investigate qualitatively
and quantitatively the bacterial aerosols before and after therapeutic

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Official Journal of the Romanian Society of Physiological Sciences
procedures within dental practices.

25. PHARMACOLOGICAL AND CLINICAL STUDYON ANTI-INFECTIOUS DRUGS IN PERINATALPERIOD
Sipos, S.
University of Medicine and Pharmacy of Timisoara- Pharmacology
Department, Romania
Introduction
In paediatric pharmacology, the perinatal period is a topicalfield that has yet to be explored more thoroughly. The firstpart of the thesis, which deals with the current stage of theknowledge of the perinatal period, comprises a study of data in
the literature of the field regarding the pharmacokinetics of thematernal-foetal unit, with an emphasis on the mechanisms ofdrug transfer across the placental barrier and the mechanismsof anti-infectious drugs excretion in the breast milk.
Objective
The second part of the thesis – personal contributions –includes clinical studies that evaluate the placental transfer ofanti-infectious drugs and their transfer in the breast milk, bydetermining their concentration in human serum and breast milkwith HPLC (high-performance liquid chromatography). Comparisons
will be made among drugs of the same class and among known
drug representatives and their modern forms. The main objectiveof the study is to establish a correlation between the levels ofanti-infectious drug concentration in maternal serum, in theumbilical cord blood and in breast milk. Therapeutic efficacy willbe estimated with specific laboratory tests both in the pregnant
woman and the new-born. The therapeutic safety profile will bemonitored by registering the possible adverse effects in relation
to the administered dose, with a view to prevent toxic effects inthe new-born. These will be correlated with the levels of drugconcentration.

26. IMMUNOGENETICS STUDIES AND THERAPEUTICCORRELATIONS IN IDIOPATHIC JUVENILE
ARTHRITIS
Somogyi-Militaru, A., Sabau, I.
“Victor Babes” University of Medicine and Pharmacy,
Timisoara, Romania
Background. Juvenile idiopathic arthritis (JIA) is the most common
chronic autoimmune disease in paediatric pathology. JIA has still
no reliable biomarkers for monitoring disease progression.
Objectives. The aim of this project is to study the correlations
between biomarkers profile of the patient and the clinical subgroupof JIA, inflammatory syndrome, radiologic modifications and theevolution under the treatment of the patient.
Study Material
The design of the project includes a retrospective and aprospective study. The retrospective study, based on a cohort of58 patients with diagnosed JIA (in the period 2004-2009, in theFirst Pediatric Clinic, Timisoara) will evaluate the prevalence ofrheumatoid factor (RF), anticyclic citrullinated peptide antibodies

Fiziologia ‑ Physiology  2010 supplement(anti-CCP) in comparison with a control group without articularpathology. These biomarkers will be correlated with the clinicalform, the inflammatory syndrome and the diseases evolution
under therapy. Treatment’s efficiency will be measured by DAS28
score, according to EULAR, and ACR score, according to ARA’s
recommendations.
The prospective study will include the new patients with
JIA. Patients will be evaluated physical, biological (inflammatory
syndrome), paraclinic (bone density DEXA), hormonal (prolactin, testosterone), immunological with determination of the two
isotopes of RF (IgA, IgM), the study of anti-CCP antibodies (IgA,
IgM, IgG), C3 and C4 components of the complement. The results
wil be compared with a control lot.
Cytokines profile, pro- and anti-inflammatory, it will be
performed using the multiplex immunoassay method, on two
lots: in children with AJI in remission respectively in patients withactive juvenile arthritis.
The major histocompatibility complex HLA (DR4, DR8, DR5,
B-27) will be studied depending on AJI subgroup.
Keywords: juvenile idiopathic arthritis, anti-CCP antibodies,rheumatoid factor, cytokines

27. THE ROLE OF MITOCHONDRIA IN PATHOGENESIS OF EXPERIMENTAL ENDOTHELIAL
DYSFUNCTION
Sturza, A.1, Muntean, D.1
Department of Pathophysiology, “Victor Babes” University of
Medicine and Pharmacy Timisoara, Romania

1

Cardiovascular disease is a leading cause of morbidity
worldwide and endothelial dysfunction (ED) represents both awidely investigated underlying mechanism and a therapeutic target.
Endothelial mitochondria have emerged as central regulators of
the calcium-dependent balance between reactive oxygen species(ROS) and nitric oxide (NO) production but the contribution of
mitochondrial damage to ED is not known. Increased production of
mitochondrial reactive oxygen species (ROS) clearly contributes to
ED pathogenesis, albeit the most relevant sources/sites are yet tobe elucidated. Recent data have incriminated monoaminooxidases(MAO) with two isoforms, A and B, as important source of deleterioushydrogen peroxide production. Increased activity of myocardial
MAO-A, the predominant isoform in the cardiovascular system, hasbeen reported to occur with ageing and contribute to both acuteventricular dysfunction associated with postischemic reperfusion,and to chronic maladaptative evolution from myocardial hypertrophy
to heart failure. However, information regarding the involvementof vascular MAO-A in cardiovascular pathology with respect toendothelial dysfunction are rather scarce.
The aims of the project is to evaluate the contribution of
ROS generated by the MAO-A isoform at the level of vascularendothelium as: (i) second messengers for eNOS activation andredox signaling and (ii) putative contributors to the development
and progression of experimental endothelial dysfunction inpathological conditions pathologies that share oxidative stressas a common denominator.
Project objectives are as follows:1) Implementation andvalidation of an experimental model to study function/dysfunctionof endothelial mitochondria (respiratory function and organ bathstudies of vascular rings); 2) Assessment of MAO-A contribution

13

Official Journal of the Romanian Society of Physiological Sciences
to the: (i) redox signalling, including NO cross-talking (if any)and (ii) pathogenesis of experimental endothelial dysfunction,
including the possibility to modulate the phenomenon with
MAO-A inhibitors.
Research partially supported by the Fellowship Project
POSDRU/88/1.5/S/63117
Key words: endothelial dysfunction, mitochondria, monoamineoxidase, nitric oxide

28. AN IN VIVO EVALUATION OF SOY ISOFLAVONOID
GENISTEIN, INCORPORATED IN RAMIFIEDCYCLODEXTRIN COMPLEX ON EXPERIMENTAL
B16 MOUSE MELANOMA
Tiulea, C.1, Peev, C.1 , Dehelean, C.2 , Soica, C.3, Feflea,
S.1, Motoc, A.4
University of Medicine and Pharmacy Victor Babes, Timisoara,
Romania, Department of Pharmacognosy
2
University of Medicine and Pharmacy Victor Babes, Timisoara,
Romania, Department of Toxicology
3
University of Medicine and Pharmacy Victor Babes, Timisoara,
Romania, Department of Pharmaceutical Chemistry4
University of Medicine and Pharmacy Victor Babes, Timisoara,
Romania, Department of Anatomy

1

Genistein is an isoflavonoid, phytoestrogenic compoundfound in soybeans, the vegetal product from the plant Glycinemax (L.) family Fabaceae. This compound is a specific inhibitor ofprotein tyrosine kinase and was found to exhibit chemopreventive,
cardioprotective and antiosteoporosis activities. One of the major
problems of this active compound is its poor water solubility.
Genistein was acquired from Extrasynthese(France),hydroxylpropyl-beta cyclodextrin (HPBCD) from Cyclolab Hungary, andB16F0 melanoma was acquired from ECACC and Sigma Aldrich,origin Japan stored UK . C57BL/6J mice were purchased fromCharles River (Germany). In order to improve the solubility ofgenistein a cyclodextrin complex was prepared. Genistein wasincorporated in hydroxyl-propyl-beta cyclodextrin complex ina molar ratio 1:2 by kneading method and a phase solubility
evaluation was prepared to observe the increasing of water solubility
for active compound. Results showed a direct relation between
the amount of cyclodextrin and the solubility of genistein. Ten
C57BL/6J mice, female of eight weeks were inoculated with 0.5
ml B16F0 melanoma cell suspension containing 0,5x105 viable
cells prepared after standard protocols on tail vein using as
solvent saline solution. Genistein in complex was ultrasonicatedinto suspension in 0.9% saline prior to injection. Mice weredivided in two groups and one group was injected with genistein
complex i.p. at daily doses of 15 mg/kg b. w. for five days fromthe first day of experiment. Animals were inspected daily forthe development of tumors, which became visible after 17 days
from inoculation, when they where sacrificed. Tissue samples

14(skin) were fixed in 10% formalin solution and were embedded
in paraffin and cut at 4 microns. Finally after deparaffinized
the samples were stained with H&E (hematoxylin-eosin) andmicroscopically analyzed. In case of untreated group it can beobserved a tumor proliferation at the level of dermal tissue
and also neighboring muscle fibers. In case of treated group
analyses showed ectaziate vessels and dermis with perivascularproliferative process consisting of round cells relatively uniform
and hyperchrom nucleus.
The main conclusions are that the water solubility of genistein
could be increased by cyclodextrin complexation and it is an active
compound in melanoma on coetaneous level.

29. METHOD VALIDATION FOR SIMULTANEOUS
MULTIANALYT DETECTION OF DRUGRESIDUES IN HONEY USING BIOCHIP ARRAYTECHNOLOGY
Popa (Morariu) ID1, Schiriac CE2, Cuciureanu, R1
University of Medicine and Pharmacy “Gr.T.Popa”, Faculty of
Pharmacy, Department of Environmental Chemistry and Food,
Iasi, Romania,
2
S.C. Investigatii Medicale Praxis S.R.L.,Biochemistry and Immunology Departament, Iasi, Romania

1

A new method for simultaneous determination of drug residues
in honey samples was validated according to the criteria set byCommission Decision 2002/657/EC. Three different immuno‑
assay based multi-analytes arrays for drug residues testing
have been evaluated: Antimicrobial Array I for simultaneousscreening of twelve sulphonamides (sulphamethazine, sulph‑
adiazine, sulphadimethoxine, sulphaquinoxaline, sulphathiazole,sulphisoxazole, sulphapyridine, sulphamerazine, sulphamethoxy‑
pyridazine, sulphachlorpyridazine, sulphamethizole, sulphadoxine),
Antimicrobial Array II for quinolones, ceftiofur, thiampheni‑col, streptomycin, tylosin and tetracyclines simultaneously,
Antimicrobial Array III for four nitrofuran metabolites andchloramphenicol simultaneously. The analytes determination
was carried out using Biochip Array Technology (Randox Laboratories
Ltd, Crumlin, Northern Ireland), an immunoassay testing platform
for the simultaneous multi-analyte assessment of a panel of tests.
All the tests on the biochip are performed simultaneously in asingle procedure using a single undivided sample. A supercooledcharged coupled device camera and customized image-processing
software simultaneously read the results for the entire panel of tests.
The method shows good linearity, sensitivity, specificity, precision(repeatability and intra-laboratory reproducibility), recovery, rug‑gedness and decision limits (CCα) below 50% of MRL (maximum
residue limits). The results of the validation process demonstratethat the method is suitable for application, as a quantitative screen‑
ing method, in European Union statutory veterinary drug residue
surveillance programs.

Fiziologia ‑ Physiology  2010 supplement

Official Journal of the Romanian Society of Physiological Sciences

AGILROM SCIENTIFIC WORKSHOP - HANDS-ON TRAININGLAB-ON-A-CHIP TECHNOLOGY - 2100 BIOANALYZER AGILENT

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Fiziologia ‑ Physiology  2010 supplement

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• „The Agilent Advantage”, Agilent Application Note, Publication
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• www.genomics.agilent.com
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Official Journal of theautHorS
Romanian Societyof Physiological Sciences
index

BALDEA, B................................................................ 5

MADA, L..................................................................10

BEGEZSAN (LOGHIN), II....................................... 5

MIRCI-DANICAR, OC...........................................10

BOLDEANU, FM...................................................... 5

MURESAN, CO.......................................................10

BOTA, S...................................................................... 6

NAICHE, D..............................................................11

BRISAN, C.................................................................. 6

PASCUT, D..............................................................11

CANDEA, A............................................................... 6

PETRESCU, E..........................................................11

CHIRILA, I.................................................................. 7

POPA, M..................................................................19

CRISTESCU, C.......................................................... 7

POPA, S....................................................................12DRAGOI, R.G............................................................ 7

ROMOSAN, R.........................................................12

DUICU, OM............................................................... 8

SERBAN, D..............................................................12

DUMITRIU (MIRON), N......................................... 8SIPOS, S...................................................................13

FLOREA, C................................................................. 8

SOMOGYI-MILITARU, A.....................................13

GHEORGHEOSU, DE............................................. 9

STURZA, A..............................................................13

ILEA, C........................................................................ 9

TIULEA, C................................................................14

IVANICA, A..............................................................10
16

Fiziologia ‑ Physiology  2010 supplement