CONTENTS
1. The results of the experimental investigations concerning the lactogenic effect of STH in sows
Iuliana Codreanu, N Constantin
2. The cytotoxic activity of A2 phospholipase produced by Fusobacterium necrophorum
Camelia Papuc, I Togoe, Aneta Pop, M Serban
3. The increase of mechanical resistance of eggshell by manipulating the physiological processes
involved in eggshell formation
Liliana Stoica, N Constantin
4. The dynamics of immunoglobuline G from blood and colostrums in sheep during peripuerperal
periodCarmen Negoita, Mariana Tetu-Oporanu, I Togoe, N Constantin
5. Aspects of acid-base balance dynamics in cerebral hypoxic hypoxia during the ozone-oxygentherapy
Michaela Rodica Perieteanu
6. Biochemical modifications determined by the cryoconservation of the bull semen
Cristina Diaconescu, Emanuela Ionescu, M Serban
7. The insulin-like action of apius graveolus lectin on BB-DP rats
Aneta Pop, Raluca Negru, R Buligescu, Camelia Papuc, Manuella Militaru, D Cheta, M Serban

1. THE RESULTS OF THE EXPERIMENTAL INVESTIGATIONS CONCERNING THE
LACTOGENIC EFFECT OF STH IN SOWS
Iuliana Codreanu, N Constantin
Faculty of Veterinary Medicine, Bucharest
Address for correspondence: Faculty of Veterinary Medicine, Splaiul Independentei No. 105, Sector 5, Bucharest,Romania

ABSTRACT
Knowing the general anabolic role of GH and its implications in inducing and developing milk
production, it has been aimed to obtain this effect for the mainly milk constituents (carbohydrates,fats, proteins) in correlation with blood biochemical parameters.
This hormone (commercial name is Norditropin) has been administered in sows, from 10 days before
farrowing and in the next 10 days after farrowing.
The study concerning the implications in milk production has been made through the ponderal
dynamic (individual and group weight) in experimental and control group. We laso tried to establish
some possible relations between quantitative expressions (through the piglets weight development)
and qualitative expression (through milk main components dosing).
Key words: GH, sows, lactation, piglets.

2. THE CYTOTOXIC ACTIVITY OFFUSOBACTERIUM NECROPHORUM
Camelia Papuc, I Togoe, Aneta Pop, M Serban
Faculty of Veterinary Medicine, Bucharest

A2PHOSPHOLIPASEPRODUCEDBY

Address for correspondence: Faculty of Veterinary Medicine, Splaiul Independentei No. 105, Sector 5, Bucharest,Romania

ABSTRACTAn A2 phopholipase was isolated from the culture supernatant of the Fusobacterium necrophorum byammonium sulfate precipitation and size exclusion chromatography. The enzyme activity was
assayes on L-α-phosphatidylcholine-β-oleoyl-γ-palmitoyl substrate and emphasized by thin layerchromatography and the cytotoxic effect was performed and demonstrated on bovine thrombocytes.
The cytotoxic activity of this enzyme is responsible for the bacterium pathogenity.
Key words: Fusobacterium necrophorum, A2 phospholipase, cytotoxicity.

3. THE INCREASE OF MECHANICAL RESISTANCE OF EGGSHELL BY
MANIPULATING THE PHYSIOLOGICAL PROCESSES INVOLVED IN EGGSHELL
FORMATION
Liliana Stoica, N Constantin
Faculty of Veterinary Medicine, Bucharest
Address for correspondence: Faculty of Veterinary Medicine, Splaiul Independentei No. 105, Sector 5, Bucharest,Romania

ABSTRACTThe tests were aimed at studying the effect of ration supplementation on laying hens, on the basis ofmineral elements having the role of enzyme activators, i.e. Mn and Zn, their quantity and rate, as
well as different organic and inorganic sources.
The results of the experiment showed that the ration supplementation by Mn and Zn in the laying
hens aged over 48 weeks has led to superior eggshell quality and reduced number of broken eggs.
The organic sources of Mn and Zn proved to be superior to the inorganic sources. The breaking
strength was 2332.33 g in the batch fed on organic sources; 2186.26 g in the batch fed on inorganic
Zn and 2169.22 g in the batch supplementary fed on mineral Mn.
Key words: eggshell, manganese, zinc, acetazolamide, shell weight, shell thickness, shelldeformation, breaking strength.

4. THE DYNAMICS OF IMMUNOGLOBULINE G FROM BLOOD AND COLOSTRUMSIN SHEEP DURING PERIPUERPERAL PERIOD
Carmen Negoita1, Mariana Tetu-Oporanu2, I Togoe1, N Constantin11Faculty of Veterinary Medicine, Bucharest
2N.S. “Institute Pasteur” R.A. Bucharest
Address for correspondence: Faculty of Veterinary Medicine, Splaiul Independentei No. 105, Sector 5, Bucharest,Romania

ABSTRACTIn ruminants, immunoglobuline G (especially IgG1) represents the main protein of sero-colostrums(80%).
Our works revealed that colostrums immunoglobuline G presented reverse fluctuations with serum
immunoglobuline G. In addition, the colostrums immunoglobuline G reached a peak 7 days
antepartum; another increase was recorded on the last 12 hours of pregnancy, then it decreased
becoming sub unitary after 72 hours of parturition.
Key words: immunoglobuline G, sheep, blood, colostrums.

5. ASPECTS OF ACID-BASE BALANCE DYNAMICS IN CEREBRAL HYPOXICHYPOXIA DURING THE OZONE-OXYGEN THERAPY
Michaela Rodica PerieteanuArmy medical Research Center, Bucharest, Romania
Address for correspondence: Michaela Perieteanu, PO 12-PB 303, Bucharest

ABSTRACT
It was conceived and verified experimental pattern for ozone-oxygen therapy of the cerebral hypoxicstress (acute).
A group of white, male rats (weight 180 ± 10 g), acute stressed by hypoxic hypoxia (10% O2 and90% N2) and cured by ozonized blood (40 mg/l O3) (HIP + O3 group) was studied comparatively
with the normal group (NOR group), and a group of identical stressed animals (HIP group).
For all the groups the following parameters were dosed, which characterized the blood acid-basebalance: blood pH, pO2 (mmHg), pCO2 (mmHg), HCO3- ions concentration (mmol/l), totalhemoglobin (tHb, g/dl).
With the regard to the acute hypoxic hypoxia: it was surveyed the dynamics of the above blood
parameters (under the presence and in the absence of treatment with ozone), exposing that thetherapy reduces significantly statistic (p<0.01) the deviations from normal and shorted (with 8 days)
the normalizing period of the values. The statistic analysis of the dosed values showed a correlatedevolution of these parameters.
Our results may be considered as a premise for the inclusion of the ozone-oxygen therapy into ananti-hypoxic set up.
Key words: stress, hypoxic hypoxia, acid-base balance, ozone-oxygen therapy.

6. BIOCHEMICAL MODIFICATIONS DETERMINED BY THE CRYOCONSERVATION
OF THE BULL SEMEN
Cristina Diaconescu, Emanuela Ionescu, M Serban
Faculty of Veterinary Medicine, Bucharest
Address for correspondence: Faculty of Veterinary Medicine, Splaiul Independentei No. 105, Sector 5, Bucharest,Romania

ABSTRACTTe present study analyzes the effects of the conservation process, on variable periods of time, onmembrane lipids, free fatty acids and on the intracellular enzyme activity: superoxide dismutase(SOD) and glutathione peroxidase (GPX) from bovine sperm.
The free fatty acids spectrum, revealed through chromatographic gas, both in seminal plasma and in
the spermatozoa extract congealed for different time periods, showed a general decrease of thepalmitic acid concentration with the increase of the time of refrigeration. It was also detected asevere reduction of the oleic acid concentration because of freezing at – 196oC for a long period oftime (12 months), decrease that was considered a measure of appreciation for the lipid peroxidation
degree of the frozen seminal material.
At enzymatic level, even after a short time (48 hours), the refrigeration produced a partial
inactivation of SOD and GPX. Their intracellular activities decreased significantly (p<0.05) anddetermined an increase of the superoxide anion (O2-) and hydroxyl radical (HO-) concentration.
Conservation through freezing in liquid nitrogen, even in optimal conditions, produces a depreciationof the semen from biochemical point of view, resulting in the decrease of the spermatozoa fertility.
Key words: frozen bovine sperm, SOD activity, lipid peroxidation.

7. THE INSULIN-LIKE ACTION OF APIUS GRAVEOLUS LECTIN ON BB-DP RATS
Aneta Pop1, Raluca Negru2, R Buligescu2, Camelia Papuc1, Manuella Militaru1, D Cheta2, M
Serban2
1
Faculty of Veterinary Medicine, Bucharest
2
Nicolae Paulescu Institute of Nutrition and Metabolic Diseases, Bucharest
Address for correspondence: Faculty of Veterinary Medicine, Splaiul Independentei No. 105, Sector 5, Bucharest,RomaniaABSTRACT
Apium graveolus (celery) leaves used in folk medicine as antidiabetic, were the source of a new
fucose lectin isolated by affinity chromatography on Fetuin-Sepharose. Purified lectin, administeredorally to 5 normal, healthy rats (0.2 mg/g body weight) induced hypoglycemia. A partially purified
lectin preparation was orally administered to 30 BB-DP rats of pre-diabetogenic ages (0.8 mg/g bodyweight) and followed for 3 months. Another group of 30 rats, BB-DP treated under the same
condition was used as control. Diabetus mellitus was diagnosed on standard criteria at 16.6% of thelectin treated group and at 30% of the animals in the control group. The lectin treatment induced adelay of the onset of the disease (97.1% days compared with 81.55 days at the control group) and adecrease of the glycemia level at the onset of the disease (316.6 mg/dl at the lectin treated groupversus 391.2 mg/dl at the control group).
Key words: diabetes mellitus, lectins, insulin-like action.