C-ProﬁleTo calculate the physicochemical
cal properties of a protein from its amino acidasequence.

Based on the amino acid sequence of a protein (FASTA format), molecular weight, pH dependence of charge/charge density,pI, ε, GRAVY, and other physicochemical properties of it can be obtained.
It is useful for puriﬁcation of protein samples and for investigation of crystallization conditions.
It is provided with a macro ﬁle that runs in Microsoft Excel.

KV FGRCELAAAMKRLHGPLAALG
CFL
ILVL
SLLMR

DFESNFNTQATN
AAKNYRNRGYSLGNWVCTDGSTFeatures
C-Proﬁle is provided as a Microsoft Excel ﬁle with macros, installed on Windows 7/10/11
Works with Microsoft Excel 2010 or later versions (32-bit and 64-bit).
The following calculations are performed and displayed from the amino acid sequence in FASTA format.
The top of the graph shows the number of charges, and the bottom of the graph shows the charge density.

・Each and total amino acid number

・

Molecular weight（average and monoisotropi・The most probable Vm value

・

Molar extinction coeﬃcientε（280nm）

・Isoelectric point (pI)・GRAVY・

Dependence of electric charge number on pH・

Dependence on electric charge density on pH

positive charge
negatice charge
Total charge

Functions
・Input amino acid sequence in FASTA format into the form ﬁeld.

・Another amino acid sequence such as peptide ligand can be input successively.

・The Vm value for the calculation of charge density can be the estimated by C-proﬁle or ﬁxed value.

・The number of Cystine (S-S bond) can be set.

・The electric charge and charge density can be calculated at given pH value.

・Conversion between absorbance at 280nm, weight concentration (mg/ml), and molarity (mM) are calculated muturaly

・A title can be given for the calculation results.

・The result can be saved in a named Excel ﬁle (.xlsx) without the amino acid sequence.

・The saved Excel ﬁle can be reloaded in C-Proﬁle to conduct all the command above.

•The change of physicochemical parameters easily, when some of the amino acids are replaced .

•According to the experimentally-obtained absorbance, the weight concentration (mg/ml) can be calculateeasily.

•The electric charge and electric density of the protein molecule at a selected pH can be estimated.

•The estimation result will be useful for e. g. the optimization of the solutions for ion-exchange chromatography
and protein crystallization.

Eﬀective usage

PriceCat. ID

Product NameQty.

Price (w/o VAT)

Content

CRT700C-Proﬁle1 set

10,000 (JPY)

CD, Ist. Manual

Restrictions
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The validity of C-Proﬁle expires one year after the activation.
C-Proﬁle can only be used and be copied in PC of the purchaser. The copy takes over the expiration data from theoriginal one.
C-Proﬁle is available for the estimation of the physicochemical property of the protein molecule only for academic
use on your own responsibility.
The results of C-Proﬁle assume no responsibility.
Neither alteration of the program nor decryption of the source code is allowed.
Transfer of C-Proﬁle to a third party is prohibited.
• The program of C-Proﬁle may be improved and/or the price of C-Proﬁle may be changed without any notice.

Confocal Science Inc.
Musashino Bld. 2F Fukasawa 5-14-15,
Setagaya-ku Tokyo, 158-0081 Japan
TEL: +81-3-5809-1561 FAX: +81-3-6411-6261
e-Mail: info@confsci.co.jp
Web: http://www.confsci.co.jp(Ver. 2407)