C-Clinic AD
Comprehensive Service for trouble shooting in protein crystallization
Proposing appropriate countermeasures for protein expression, crystallization,structural analysis, and others.

1 『Providing crystallization reagents and devices』
Hearing the problems in the crystallization, Confocal Science will provide with several appropriate
crystallization reagent solutions. C-Kit Ground Pro XRD*1 is necessary for the crystallization
devices.
Customer provides: amino acid sequence of protein (FASTA format)
and crystallization problems
It is not necessary to disclose the details of the protein or to provide aprotein sample. If it is difficult to disclose the amino acid sequence, thephysicochemical properties of the protein sample estimated by C-Profile*2
is necessary.
◆Solution delivery: ¥40,000 ～ 60,000 (w/o VAT) / 1 time (4～6 species）◆Disclosure of solution compositions: ¥300,000 (w/o VAT)/ 1 species『Evaluation of the protein sample』
Evaluate protein samples for suitability for crystallization and isothermal titration calorimetry (ITC).
Based on biochemical analysis and physicochemical properties estimatedfrom the amino acid composition of the protein sample, as well as
empirical evaluation, Confocal Science will suggest ways to improve or
purify the sample.

SDS-PAGE Native-PAGE

Items provided by the customer: Protein sample (about 0.5 mg)
Amino acid composition of proteins※ If it is difficult to disclose the amino acid sequence, thephysicochemical properties of the protein sample estimated by C-Profile*2
is necessary.
◆Cost: ¥900,000（w/o VAT）

DLS

3 『Examination of the purification methods』
Examine purification method of protein samples to make them suitable for crystallization or ITC.

After conducting “Evaluation of protein samples”, a suitable purification
method will be proposed.
Items provided by the customer: Protein sample (about several tens mg)
Amino acid composition of proteins
クロマトによる精製◆Cost: Estimated based on the result of “Evaluation of the proteinsample”.

4 『Optimization of crystallization conditions for XRD』
Optimize crystallization conditions to obtain a crystal for X-ray diffraction and confirm its quality.
Confocal Science optimizes the crystallization conditions to obtain acrystal for X-ray diffraction experiment. If a good shaped crystal isobtained, the crystallization condition and method will be reported. Ifrequested, X-ray diffraction data will be collected to confirm the diffraction
quality of the crystals.
Items provided by the customer: Protein sample (about several tens mg)
Amino acid composition of proteins◆Cost: Estimated based on the result of “Evaluation of the proteinsample”.

5『Optimization of conditions for a large crystal』
Optimization of crystallization conditions for large crystals for neutron diffraction experiments.

Confocal Science optimize the crystallization conditions to obtain a large
crystal for neutron diffraction experiment. If a good and large crystal isobtained, the crystallization condition and method will be reported. Ifrequested, X-ray diffraction data will be collected to confirm the diffraction
quality of the crystals.
Items provided by the customer: Protein sample (about several tens mg)
Amino acid composition of proteins◆Cost: Estimated based on the result of “Evaluation of the proteinsample”.
C-Tube LCDM

※1 C-Kit Ground Pro XRD: A protein crystallization kit for X-ray diffraction experiments.
※2 C-Profile: A program that calculates the basic physicochemical properties of a protein from its
amino acid sequence.

Cat. ID.

ProductName

Qty.

Price(w/o VAT)

Content

CRT101-1

C-Kit Ground
Pro XRD

1 set¥50,400-

A set of experimental devices

CRT700C-Profile

1 set¥10,000-

CD, Instruction Manual

Confocal Science Inc.
Musashino Bld. 2F. Fukasawa 5-14-15, Setagaya-ku
Tokyo, 158-0081 Japan
TEL: +81-3-5809-1561 FAX: +91-3-6411-6261
e-Mail： info@confsci.co.jp
Web: http://www.confsci.co.jp/(Ver.2406)