⎇ⓥ⺖㗴ฬ㧦⥄↱㔚ሶ࡟࡯ࠩ࡯ߩ⚵❱ౣ↢ലᨐߦ㑐ߔࠆ⎇ⓥ⎇ⓥઍ⴫⠪㧦ᷡ᳓ ౖ૫ (ᣣᧄᄢቇ ᱤቇㇱᱤቇ⑼)⎇ⓥᓥ੐⠪㧦⍹ਣ ㅘሶ (ᣣᧄᄢቇ ᱤቇㇱᱤቇ⎇ⓥ⑼⥃ᐥ♽ኾ᡹)㚍⍫ේℙᨑ (ᣣᧄᄢቇ ᱤቇㇱᱤቇ⎇ⓥ⑼⥃ᐥ♽ኾ᡹)౒ห⎇ⓥ⠪㧦਄↰ ␭ੑ (ᣣᧄᄢቇ ᧻ᚭᱤቇㇱᱤቇ⑼)‫⎇ޣ‬ⓥ⋡⊛‫ޤ‬ૐ಴ജ࡟࡯ࠩ࡯ߦߪഃ்ᴦ≹૞↪߿‫ޔ‬㛽ୃᓳଦㅴ૞↪߇޽ࠆߎߣ߇ႎ๔ߐࠇߡ޿ࠆ‫ޕ‬ᚒ‫ ߽ޘ‬invivo, in vitro ߩታ㛎ߢૐ಴ജඨዉ૕࡟࡯ࠩ࡯ߦ㛽ᒻᚑଦㅴ૞↪߇޽ࠆߎߣࠍႎ๔ߒߡ޿ࠆ‫ޔߒ߆ߒޕ‬࡟࡯ࠩ࡯శߪᾖ኿㊂‫ޔ‬ᾖ኿ኒᐲ‫ޔ‬ᵄ㐳‫ࠬ࡞ࡄޔ‬᏷‫ޔ‬ᾖ኿ᤨᦼ╬ߦࠃࠅ↢‛ቇ⊛૞↪߇⇣ߥࠆߣ⠨߃ࠄࠇࠆߚ߼‫ߥ߁ࠃߩߎޔ‬ᾖ኿᧦ઙߩ㆑޿ߦࠃࠆ㛽ୃᓳലᨐࠍᬌ⸛ߒ‫ᦨޔ‬ᄢߩ㛽ᒻᚑଦㅴലᨐࠍ⊒ើߔࠆᾖ኿᧦ઙࠍ⷗಴ߒߚ޿ߣ⠨߃ߚ‫⺃❱⚵ߩ࡯ࠩ࡯࡟ޔߦࠄߐޕ‬ዉଦㅴࡔࠞ࠾࠭ࡓࠍ⸃᣿ߔࠆߎߣ
ߦࠃࠅૐ಴ജ࡟࡯ࠩ࡯ߩᱤ⑼⥃ᐥᔕ↪ࠍߪ߆ࠅߚ޿‫ޕ‬‫⎇ޣ‬ⓥ᭎ⷐ‫ޤ‬
Ga-Al-As ඨዉ૕࡟࡯ࠩ࡯ᾖ኿ེ(830nm,500mW)ࠍ↪޿ߡ㛽⧘⚦⢩ߦㅪ⛯ᵄ㧔CI㧕ߣ 1Hz ࡄ࡞ࠬᵄ(PI-1)ߩᾖ኿ࠍⴕ޿‫࡯ࠩ࡯࡟ޔ‬ᾖ኿ߦࠃࠆ㛽ᒻᚑଦㅴ૞↪ߦߟ޿ߡᬌ⸛ߒߚ‫ޕ‬ᅧᆼ㧞㧝ᣣ⋡ߩ࡜࠶࠻⢝੿߆ࠄࠦ࡜ࠥ࠽࡯࠯ࠍ↪޿࡜࠶࠻⢝੿↱᧪㛽⧘⚦⢩ࠍណขߒ‫ޔ‬หኒᐲߢၭ㙃ߒߚ‫ޕ‬ၭ㙃㛽⧘⚦⢩ߦ 0.48㨪3.84J/cm2 ࡟࡯ࠩ࡯ᾖ኿ࠍⴕ޿‫⚦ޔ‬⢩Ⴧᱺ‫ޔ‬㛽⧘⚦⢩ಽൻࡑ࡯ࠞ࡯ߩࠕ࡞ࠞ࡝ࡎࠬࡈࠔ࠲࡯࠯㧔ALP㧕ᵴᕈ‫ޔ‬ALP ㆮવሶ⊒⃻‫ ߮ࠃ߅ޔ‬bone nodule ᒻᚑࠍᬌ⸛ߒߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬ኻᾖ⟲ߦᲧߴᾖ኿⟲ߢߪ‫ޔ‬ၭ㙃㧢㧘㧥ᣣ⋡ߢ⚦⢩Ⴧᱺ߇Ⴧᄢߒ‫ޔ‬㧥㧘㧝㧞㧘㧝㧡ᣣߢߪ ALP ᵴᕈ߇Ⴧᄢߒ‫ޔ‬㧞㧝ᣣ㑆ߩታ㛎ߢߪ bone nodule ߩᢙߣ㕙Ⓧ߇᦭ᗧߦჇᄢߒߚ‫ߚ߹ޕ‬ᾖ኿㊂ߦᔕߓߡ bone nodule ᢙ߽Ⴧᄢߒߡ޿ߚ‫ߩࠄࠇߎޔߦࠄߐޕ‬ലᨐߪ CI ߦᲧߴ PI-1 ߇᦭ᗧߦჇᄢߒߡ߅ࠅ‫ޔ‬㑆᱉⊛ߥశೝỗ߇㛽ᒻᚑߦ᦭ലߢ޽ࠆߎߣ߇␜ໂߐࠇߚ㧝㧕‫ޕ‬ᰴߦ࡜࠶࠻⢝੿↱᧪㛽⧘⚦⢩ࠍ↪޿ߚห᭽ߩታ㛎ߢ‫ޔ‬CI, PI-1 ߩ߶߆ߦ㧞㧘㧤Hz ߩࡄ࡞ࠬᵄ(PI-2,-8)ߩᾖ኿ࠍⴕ޿Ყセᬌ⸛ߒߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬ኻᾖ⟲ߦᲧߴߔߴߡߩᾖ኿⟲ߢ⚦⢩Ⴧᱺ‫ޔ‬ALP ᵴᕈ߅ࠃ߮ㆮવሶ⊒⃻‫ޔ‬bone nodule ᢙ‫ޔ‬㕙ⓍߪჇᄢߒߡ޿ߚ߇‫ ߪࠄࠇߎޔ‬CI ߿ PI-8 ߦᲧߴ PI-1,-2߇᦭ᗧߦჇᄢߒߡ޿ࠆߎߣ߇ࠊ߆ߞߚ‫ޔࠄ߆ߣߎߩߎޕ‬ၭ㙃㛽⧘⚦⢩ߦኻߔࠆඨዉ૕࡟࡯ࠩ࡯ᾖ኿ߩ㛽ᒻᚑଦㅴ૞↪ߪ‫ޔ‬㧝޽ࠆ޿ߪ 2Hz ߩㆃ޿ࡄ࡞ࠬᵄ߇ㅪ⛯ᵄ߿ᣧ޿ࡄ࡞ࠬᵄࠃࠅ᦭ലߢ޽ࠆߎߣ߇ࠊ߆ࠅ‫ࠬ࡞ࡄޔ‬㗫ᐲ߇⚦⢩ߩ↢‛ቇ⊛ᵴᕈߦᓇ㗀ࠍਈ߃ߡ޿ࠆߎߣ߇ࠊ߆ߞߚ 2)‫ޕ‬ ᰴߦ‫⚦ߩ࡯ࠩ࡯࡟ޔ‬⢩ᗵฃᕈߦ㑐ߔࠆታ㛎ࠍⴕߞߚ‫ޕ‬ಽൻ⒟ᐲߩ⇣ߥࠆ㧞⒳㘃ߩ࡜࠶࠻㗡⬄౰↱᧪㛽⧘⚦⢩᭽ᩣൻ⚦⢩‫ᧂޔ‬ಽൻဳ C-26 ⚦⢩ߣಽൻဳ C-20 ⚦⢩ࠍၭ㙃ߒ‫ޔ‬Ga-Al-As ඨዉ૕࡟࡯ࠩ࡯
ᾖ኿ (830nm,500mW)ࠍⴕ޿‫⚦ޔ‬⢩ߩ ALP ᵴᕈࠍ᷹ቯߒߚ‫ޕ‬ᾖ኿ߪ 3.82J/cm2 ࠍ㧝࿁ߣߒᢙ⒳ߩᾖ኿
ࠍⴕߞߚ‫⚿ߩߘޕ‬ᨐ‫ᧂޔ‬ಽൻဳߩ C-26 ⚦⢩ߢߪ᠞⒳ᓟ㧝ᣣ‫ޔ‬㧟ᣣ⋡ߦ㧝࿁ᾖ኿ࠍⴕ߁ߣ㕖ᾖ኿⟲ߦᲧセߒߘߩᓟߩ ALP ᵴᕈ߇᦭ᗧߦ਄᣹ߒߚ߇‫ⶄޔ‬ᢙᾖ኿ߢߪߘߩലᨐߪ⷗ࠄࠇߥ߆ߞߚ‫৻ޕ‬ᣇ‫ޔ‬ಽൻဳߩ C-20 ⚦⢩ߢߪ‫ⶄޔ‬ᢙᾖ኿ࠍ฽߼ߤߩࠃ߁ߥ᧦ઙߢ߽ ALP ᵴᕈߦᄌൻߪߥ߆ߞߚ‫ߎߩߎޕ‬ߣࠃࠅ‫ޔ‬ඨዉ૕࡟࡯ࠩ࡯ᾖ኿ߪᧂಽൻߥ⚦⢩ߦᾖ኿ߔࠆߎߣߢಽൻࠍଦㅴߔࠆᯏ⢻ࠍ᦭ߔࠆߣ⠨߃ࠄࠇ‫⥋ޔߚ߹ޔ‬ㆡᾖ኿㊂߇ሽ࿷ߒ‫ޔ‬ㆊ೾ᾖ኿ߢߪലᨐ߇ߥ޿ߎߣ߇␜ໂߐࠇߚ 3)‫ޕ‬ߐࠄߦ‫ޔ‬ඨዉ૕࡟࡯ࠩ࡯ᾖ኿ߩ㛽ᒻᚑࡔࠞ࠾࠭ࡓࠍᬌ⸛ߔࠆߚ߼ߦ‫࠻࠶࡜ޔ‬⢝੿↱᧪㛽⧘⚦⢩ࠍណขߒ‫ޔ‬਄⸥ห᭽หኒᐲߢၭ㙃ߒߚ‫ޕ‬ၭ㙃㛽⧘⚦⢩ߦ 3.82J/cm2㧝࿁ᾖ኿ࠍⴕ޿‫ޔ‬㛽ߩᚑ㐳࿃ሶߢ޽ࠆ insulin-like growth factor (IGF-I)-I ߩ࠲ࡦࡄࠢ෸߮ㆮવሶ⊒⃻ߦߟ޿ߡᬌ⸛ߒߚ‫࠽ࡆࡦࠦ࡝ߚ߹ޕ‬ࡦ࠻(r)IGF-I ߿ IGF-I ᛫૕ࠍ૞↪ߐߖߡ࡟࡯ࠩ࡯ᾖ኿ߒߚᤨߩ bone nodule ᒻᚑ⢻ߦߟ޿ߡ߽ᬌ⸛ࠍⴕߞߚ‫⚿ߩߘޕ‬ᨐ‫(ޔ‬r)IGF-I ૞↪⟲ߢߪ bone nodule ᒻᚑߪ㗼⪺ߦଦㅴߐࠇߚ‫࡯ࠩ࡯࡟ޕ‬ᾖ኿ߦࠃࠆ
bone nodule ᒻᚑଦㅴ૞↪ߪ IGF-I ᛫૕ࠍ૞↪ߐߖࠆߣኻᾖ⟲࡟ࡌ࡞߹ߢૐਅߒߚ‫ޔߚ߹ޕ‬IGF-I ߩ࠲ࡦࡄࠢ෸߮ㆮવሶ⊒⃻ߪᾖ኿ᓟߦ㗼⪺ߦჇᄢߒߚ‫ޕ‬એ਄ߩߎߣ߆ࠄ‫࡯ࠩ࡯࡟ޔ‬ᾖ኿ߦࠃࠆ bonenodule ᒻᚑଦㅴ૞↪ߪ‫ޔ‬㛽⧘⚦⢩߇↥↢ߔࠆ IGF-I ߩㆮવሶ߅ࠃ߮࠲ࡦࡄࠢ⊒⃻ࠍ੺ߒߡ⿠ߎߞߡ޿ࠆߎߣ߇ࠊ߆ߞߚ‫ޕ‬‫ޤ߼ߣ߹ޣ‬ ᧄ⎇ⓥߢ࡟࡯ࠩ࡯ᾖ኿ߦࠃࠆ↢‛ቇ⊛⚦⢩ᔕ╵ᕈߦߟ޿ߡ޿ߊߟ߆ߩ⚿ᨐ߇ᓧࠄࠇߚ߇‫ߦ⚦⹦ޔ‬ߟ޿ߡߪ޿߹ߛਇ᣿ߥὐ߇ᄙ޿‫੹ޕ‬ᓟߪࡅ࠻㗶㛽߆ࠄណขߒߚ㛽⧘⚦⢩ࠍၭ㙃ߒ‫ޔ‬⒳‫᧦ߩޘ‬ઙߢ⥄↱㔚ሶ࡟࡯ࠩ࡯ᾖ኿ࠍⴕ޿‫ޔ‬bone nodule ᒻᚑࠍᦨᄢߦଦㅴߔࠆ᧦ઙࠍ⷗಴ߔ‫᧦ߩߘޕ‬ઙߢၭ㙃㛽⧘⚦⢩ߦห᭽ߦ࡟࡯ࠩ࡯ᾖ኿ࠍⴕ޿‫⚦ޔ‬⢩߆ࠄ mRNA ࠍ࿁෼ߒ‫⸃ࠗ࡟ࠕࡠࠢࠗࡑޔ‬ᨆࠪࠬ࠹ࡓࠍ↪
޿ᄙߊߩㆮવሶ⊒⃻࡟ࡌ࡞ߩᄌൻߣ⚦⢩ౝࠢࡠࠬ࠻࡯ࠢࠍᬌ⸛ߒ‫ߩ࡯ࠩ࡯࡟ޔ‬㛽ᒻᚑࡔࠞ࠾࠭ࡓߦ
ߟ޿ߡ⸃᣿ߒߚ޿ߣ⠨߃ࠆ‫ߥ߁ࠃߩߎޕ‬ᬌ⸛ࠍട߃ࠆߎߣߢ‫ߩ࡯ࠩ࡯࡟ޔ‬ᱤ⑼⥃ᐥᔕ↪ࠍߪ߆ࠅߚ޿‫ޕ‬‫⎇ޣ‬ⓥᬺ❣╬‫ޤ‬࡮⊒⴫⺰ᢥ(1) Y Ueda and N Shimizu (2001) Pulse irradiation of low-power laser stimulates bone nodule formation. JOral Science 43, 55-60(2) Y Ueda and N Shimizu (2003) Effects of pulse frequency of low-power laser therapy (LLLT) on bonenodule formation in rat calvarial cells. J of Clin Laser Med Surg 21, 271-277(3) ⍹ਣㅘሶ(2003)ૐ಴ജ࡟࡯ࠩ࡯ᾖ኿߇㛽⧘⚦⢩᭽ᩣൻ⚦⢩ߩࠕ࡞ࠞ࡝ࡎࠬࡈࠔ࠲࡯࠯ᵴᕈߦਈ߃ࠆᓇ㗀 ᣣᄢᱤቇ 77, 293-300(4) Shimizu N, Ozawa Y, Ueda Y, Ishimaru T, Mayahara K, Iwase T, Moro T, Abiko Y:

Low-intensity laser

irradiation stimulates bone nodule formation via insulin-like growth factor-I expression in rat calvarialcells㧔ᛩⓂḰ஻ਛ㧕࡮⪺ᦠ(1) ᷡ᳓ౖ૫(2003) ⍶ᱜߣ࠰ࡈ࠻࡟࡯ࠩ࡯, ࡟࡯ࠩ࡯ߩ㛽ᒻᚑଦㅴ૞↪, ࡟࡯ࠩ࡯⥃ᐥ߹ࠆߎߣᄢㄉౖ ╙㧝, ࠺ࡦ࠲࡞࠳ࠗࠕࡕࡦ࠼␠, ᧲੩, 130-133࡮ቇળ⊒⴫(1) ⍹ਣㅘሶ‫ޔ‬ਛ⷏ℙᨑ‫ޔ‬ᄢጊᐘᴦ‫᧛⚊ޔ‬᤭ศ‫ޔ‬ᷡ᳓ౖ૫(2003.10) ૐ಴ജ࡟࡯ࠩ࡯ᾖ኿߇⇣ߥࠆ
ಽൻ⒟ᐲߩ㛽⧘⚦⢩᭽ᩣൻ⚦⢩ߩࠕ࡞ࠞ࡝ࡎࠬࡈࠔ࠲࡯࠯ᵴᕈߦਈ߃ࠆᓇ㗀 ╙ 62 ࿁ ᣣᧄ⍶
ᱜᱤ⑼ቇળᄢળ㧔ᣂẟ㧕(2) Shimizu N, Ozawa Y, Ueda T, Ishimaru T, Iwase T, Moro I, Abiko Y, Namura S (2002) Low-intensity
laser irradiation stimulates bone nodule formation via IGF-I gene expression in rat calvarial cells. The 8thInternational Congress in Lasers in Dentistry, (Yokohama, Japan)࡮ቇ૏ߩขᓧ⁁ᴫ(1) ⍹ਣㅘሶ: ඳ჻(ᱤቇ), ૐ಴ജ࡟࡯ࠩ࡯ᾖ኿߇㛽⧘⚦⢩᭽ᩣൻ⚦⢩ߩࠕ࡞ࠞ࡝ࡎࠬࡈࠔ࠲࡯࠯ᵴᕈߦਈ߃ࠆᓇ㗀, 2003 ᐕ 3 ᦬, ᣣᧄᄢቇᄢቇ㒮ᱤቇ⎇ⓥ⑼⥃ᐥ♽ኾ᡹