eEF2Control:
Control:
eEF2
2+2+
Ca
Ca
cAMP
cAMP
mTORC1mTORC1,UVLight
Light(GCN2)(GCN2)UV
HemeDeficiency
Deficiency(HRI)
(HRI)
Heme
HeatShock,
Shock,ER
ERStress,
Stress,Heat
Hypoxia(PERK)(PERK)
Hypoxia

ON
ON

DHX29
DHX29

InitiationCodon
Codon
Initiation
Recognition
Recognition

Factor
FactorRelease
Release

Cap
Cap

AUG
AUG

OFF
miRNA OFF
miRNA

Cap
Cap
AA(n)
(n)

eIF1
eIF1

F2K
F2K

48S
48S

Scanning
Scanning

48S
48S

AUG
AUG

Initiation
Initiation

eIF4B
eIF4B

Initiation
Initiation
Complex
Complex

AA(n)
(n)

eIF5
eIF5

43S
43S
eIF4F
eIF4F

eIF2
eIF2

Cap
Cap

StressGranules
GranulesStressP-bodies
P-bodies

GDP
GDP

eIF3
eIF3

PABPAA
PABP

(n)
(n)

eIF6
eIF6

AUG
AUG

eIF2
eIF2
Met-tRNAi
Met-tRNAi

GTP
GTP

miRNA
miRNA

eIF1A
eIF1A

40S
40S

Cap
Cap

60S
60S

GTP
TP

AA(n)
(n)

AUG
AUG

GTP
GTP

eIF1A
eIF1A
Doing
good science is important.

eIF1
eIF1

60S
60S

So is just doing good.
eIF5
eIF5

eIF5B
eIF5B

eEF1
eEF1
eEF2
eEF2

eIF5B
eIF5B

Elongation
Elongation

GDP
GDP

eIF3
eIF3

GTP
GTP

40S
40S
AUG
AUG

A(n)
A(n)

60S
60S

ERLumen
Lumen
ER

6060
SS
Polypeptide
Polypeptide
Chain
Chain

4040
SS

40S
40S

A
AAAA
AAAAA

Nascent
Nascent
Polypeptide
Polypeptide

Cap
Cap

Termination
Termination

eRF1
eRF1
eRF3
eRF3
GTP
GTP
rev.01/16/20
01/16/20
rev.

This multiplex IHC analysis enables observation of CD8 (#70306, magenta) T cells with three immune
checkpoint receptors: PD-1 (#43248, green), LAG-3 (#15372, orange), and TIM-3 (#45208, yellow) in
non-small cell lung cancer tissue. The Pan-Keratin antibody (#4545, cyan) facilitates visualization oftumor cells, while CD68 (#76437, red) enables observation of macrophages.

2

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

A company of problem solvers.
A culture of caring.
Our mission at Cell Signaling Technology (CST) has always been about
advancing science, first and foremost. Do researchers around theworld have the tools they need to get the answers they’re after? Whatgaps can we help fill? What solutions can we provide? Each discovery
made becomes knowledge that benefits all.
CST is a privately held, family-owned company—a company dedicatedto innovation and enabling positive change. And when you have thatmindset, you naturally see other ways to make the world better. So in
addition to the things we do for our Customers, we work to be partof the solution for environmental challenges facing our planet. For
disadvantaged and underserved members of our community. For
young scientists who need access to better science education. And forthe members of our own CST family around the world.
Beyond innovating to create new research tools, what else can we do?
What else can we give? For all of us at Cell Signaling Technology, these
are the central questions we look to answer every day.

•3

Scientists who put science first.

As a company founded
and led by researchscientists, we’re
working to help unlock
the secrets of life.
To empower the global scientificcommunity, accelerate biologicalunderstanding, and enable
personalized medicine. And webelieve in the power and promiseof science to solve the challenges
we face as a global community.
Because we’re scientists, weunderstand your needs as a
researcher. We know what it takes
to achieve meaningful results—
the patience, rigor, sacrifice, anddedication. And we know you haveto be able to rely on the tools you
use in your investigations, whether
in an academic setting or anindustry one.
That’s why we’re so fanatical
about the quality of our antibodies
and other products. And why
we’re dedicated to providing ourCustomers with the best possible
service and support. Engaging insolutions-focused consultation andsupport is a foundational part of
who we are and what we do.
Every day, we work to develop
innovative, proven solutions that canhelp you find the answers you’relooking for—answers that can lead
to better understanding, diagnosis,
and treatment of disease.

4

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

Solutions you can trust.

Arindam Bose, M.S., PhD
Lead Scientist –Spatial Proteomics
Leica Microsystems

Vital Statistics

Founded in 1999 by
Michael J. Comb, PhD

Products distributed
in 61+ countries

Headquarteredin Danvers,
Massachusetts,
U.S.A.

Regional offices inShanghai, China;
Tokyo, Japan;
Leiden,The Netherlands

Over 600
employees
worldwide

178+ CST scientificpublications inpeer-reviewed
journals

I feel that CST is the embodiment of a perfect company. The validation of antibodies thatyou painstakingly do is really important for customers. But not only that—socially, youhave a big presence in making sure that you’re giving back to the community. And fromthe standpoint of global change, you’re putting your money where your mouth is andreally focused on issues that matter . . . So you’re not only an antibody company, you’realso driving science, which I think is really amazing.

•5

Our name says Cell Signaling.

CST got its start incellular signaling–and
we’re still a world leader
in research productsthat help scientists
better understandsignal transduction.
But today’s CST offers you a lot
more. Our ever-expanding portfolio
includes best-in-class antibodies,
products, and services for a wide
range of applications and humandisease research areas. In addition
to our antibodies and reagents,
we can provide you with assay
kits, custom conjugation services,proteomics services, and manyother tools and services aimed atmoving your research forward.
CST was first to market with many
important products, including
ALK, ROS1, PDL-1 antibodies, andPTMScan® proteomics. Newer
products like our CUT&RUN kit,
CAR linker antibodies, andSignalStar™ Multiplex IHC have also
been widely lauded. And we’re
working with a number of platform
providers in exciting areas likespatial biology to help develop newtechnologies aimed at ensuring you
get reliable results faster.
There’s no limit to where CST may
go next, because our journey
as a company is defined not bycommercial goals but by ourlongstanding commitment to beinga resource that you can rely on,
wherever your investigations may
take you.

6

Scientists have recently demonstrated the importance of elucidating spatial localization andproximity of immune cells expressing immune checkpoints in the tumor microenvironment.
Shown here are tumor cells stained with PD-L1 XP® Rabbit mAb #13684 in yellow, as well as T
cells stained with PD-1 XP Rabbit mAb #86163 in green.

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

But that’s just the beginning.

Products & Services•

Best-in-Class Research Antibodies•

Immunoassay Kits•

Antibody Formulation & Conjugation•

Proteomic Analytical Services•

Application Workflow Reagents

Applications Supported•

Immunohistochemistry•

Spatial Biology•

High-Content Analysis

•

ELISA

•

Immunofluorescence•

Flow Cytometry•

ChIP & ChIP-seq•

CUT&RUN and CUT&Tag•

Immunoprecipitation•

Western Blot & Simple Western

Disease Areas•

Oncology & Immuno-Oncology•

Alzheimer’s, Parkinson’s & Other
Neurodegenerative Diseases•

Metabolic Disease•

Cardiovascular Disease•

Autoimmunity & Inflammation•

Fibrotic Disease

Resources for Your
Research

Diya Binoy Joseph, PhD
Scientist,
DBT-InStem

I can be sure that when I use a CST®
product that it will work almost 100% of thetime. We do a lot of immunostaining andflow cytometry, so the antibodies are very
crucial there. To have reliable antibodies
that are already optimized for a particularapplication, that makes it much easier for me.

•

Interactive Pathway Diagrams•

Technical Videos & Webinars•

Assay Protocols•

Application Guides•

Scientific and Educational Posters•

7

CST® products measure up to the highest
standards.
You need results that areunambiguous, specific,
and reproducible.
Our industry-leading validation
process means that we canguarantee our antibodies will
work as expected in your assays.
Every time. Because unlike othercompanies, we make more than
95% of our antibodies in-houseand control all aspects of theantibody development process.
The truth is, an antibody’s
specificity in one application
doesn’t mean that it will performreliably in others. That’s why we
don’t rely on a single assay toevaluate an antibody, and that
includes knockout validation.
Instead, we validate our antibodies
independently in each application—and guarantee them to work inthose applications.
The results of our painstaking
process speak for themselves. Of
the 10 antibodies most often cited inpeer-reviewed publications, six are
from CST—and more than one-thirdof the top 100-cited antibodies areours, including the number-one
most-cited primary and secondary
antibodies.* Because when it
matters most, more researchers
trust CST antibodies to give you thedefinitive, reproducible results youneed to succeed in your journey of
discovery.

*CiteAb, “What were the 100 research antibodies
of 2022?” July, 2023

8

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

Ours.

Our Unique Approach to Antibody Validation
CST tailors a combination of validation strategies applied to each antibody. Thismeans customizing our validation process according to the biological role of thetarget, while considering the sensitivity requirements of the downstream assay, theavailability of appropriate testing models, and the relevance of each application totarget investigation.
To ensure our antibodies will work in your experiment, we validate our antibodies ineach application independently, and adhere to the Hallmarks of Antibody Validation™,
six complementary strategies used to determine the functionality, specificity, andsensitivity of an antibody in a given assay. By performing validation in the same
application you plan to use for your experiment, we can guarantee that the antibody
will perform as expected, every time. We will never recommend an antibody for any
application for which it has not passed our stringent validation process .

Antibodies are Validated in Each Application Independently

Application 1

Antibody
development
for desired
applications

Application 2

Application 3

Pass

BinaryStrategy
Complementary
Strategies

RangedStrategy

Heterologous
Strategy

Orthogonal
Strategy
Multiple Antibody
Strategy

Fail

Antibody
approved foruse in validated
applications

Pass

Hallmarks of Antibody Validation

Sullim Lee, PhD
Gachon University,
South Korea

I choose CST® products based on publishedpapers and colleague’s recommendations.
They’re application-specific, and their results
are specific and reproducible. I know I canwork faster and better thanks to CST, because
I don’t have to worry about the antibodies’
function and reproducibility.

•

9The planet needs our help.

Our planet and all
who inhabit it face amultitude of challenges.
As scientists, we consider it our
duty to help protect the Earth’s
ecosystems, combat climatechange, and safeguard biodiversity.
We also believe in supportingorganizations that help theunderserved. These priorities havebeen cornerstones of our company
values since our inception.
At work, that means innovating
to reduce our carbon footprint,
operating our businesssustainably, and encouraging our
employees worldwide to workand live more sustainably. In ourcommunities, it means working
to create positive change forour neighbors, inspiring the next
generation of scientists, promoting
employee volunteerism, and more.
CST has set an ambitious, sciencebased goal of achieving net-zeroemissions by 2029. We are thefirst life science member of 1%
for the Planet. And we’re active
in supporting and promoting
nonprofit partners that areleading the movement towardgreener labs. Altogether, CST
helps fund the work of more than
110 nonprofits around the world.
We view these commitments asnecessary investments in ethicalbusiness practices for the future of
our planet and every living thingthat calls it home.

10

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

So do our neighbors.

Environmental andSocial Responsibility•

First Life Science Member of 1% for
the Planet•

Committed to Net-Zero Emissions
by 2029•

Sustainable & Recyclable Packaging•

Energy & Waste Management
Programs•

Ongoing Commitment to
Sustainable Operations•

Grants for EnvironmentalCommunity-Science Education•

Science Scholarship Programs•

Rising Black Scientists Awards(with Cell Press & Elsevier)•Support for Arts Communities•

Employee-Driven Volunteering and
Giving Programs

CST is proud to be the first life science
member to join the 1% for the Planet
network, through which we support
nonprofit organizations around theworld that are addressing our mostpressing social and environmental
issues. The commitment means that wecontribute 1% of our annual revenue tomember nonprofits, and work to inspireindustry peers and customers to join
the movement.

Kate Williams,
CEO,
1% for the Planet

CST definitely walks the walk. They came to us with intentionality. They give thoughtfully. They
engage their employees in the process . . . It’s an extremely credible commitment that they’re making
with 1% for the Planet, and it’s part of a larger journey that they’re on to do business differently, andline up their business with what’s good for the planet, for people, for their employees, and reallyacross the whole ecosystem.

•

11

More than a company.

We believe that ourcompany’s most valuableassets are our people,
whatever their roles andwherever they live.
From our inception, CST has beenunique in the ways that we’ve
supported and engaged with our
employees, both at work and intheir personal lives. This support
goes far beyond providing the
traditional benefits one expects
from an employer—and translates
into meaningful benefits for ourcustomers. Our employees areproud to work at CST, and it showsin the work they do.
Prioritizing things like diversity,
equity, inclusion, and work-lifebalance is nothing new to us. We
educate one another via weekly
scientific seminars. We support
each other through employeerun resource groups. We promoteeach other’s health and wellbeing through wellness programs
and company-paid health clubmemberships. Employee-run
committees choose our grantrecipients and scholarship winners,
too, while we volunteer together
to support important causes being
championed by CST employees allaround the world.
During the COVID-19 pandemic,
not a single CST employee waslaid off or suffered any reduction insalary to mitigate the pandemic’s
impact on our business. Because
it isn’t enough just to say that you
care about your employees. In thiscontext as in so many others, actions
truly speak louder than words.

12

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

A family.

Angelina Calnan,
Research Associate,
CST

Global Employee
Engagement Programs•

Diversity, Equity & Inclusion
Initiatives•

Employee-Run Committees & Giving
Decisions•

Scientific Seminars & Weekly Lecture
Series•Employee Donations Match
Programs•

Reward & Recognition Programs•

Employee Resource Groups•

Alternative Transportation
Incentives•

Volunteering Opportunities•

Social Events & Employee Concerts•

Art Exhibits & Receptions•

Employee Wellness Programs &Events•Company-Paid, Plant-Based Dining
Options

I’ve worked at CST for four and a half years now, and it’s amazing to see the company evolve.
They have offered resources that other employers definitely do not, and they help guide
employees through obstacles throughout their life and in work. And I truthfully feel that theresource groups have propagated such a beautiful, diverse environment.

•

13This multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma shows PD-L1
XP® rabbit mAb #13684 (red), PD-1 XP rabbit mAb #86163 (green), LAG3 XP rabbit mAb #15372 (magenta),
TIM-3 XP rabbit mAb #45208 (yellow), CD8α mouse mAb #70306 (orange), and Pan-Keratin mouse mAb#4545 (cyan).

14

For Research Use Only. Not for Use in Diagnostic Procedures.

|

cellsignal.com

You’re on a mission.
We are, too.
Scientific inquiry is about asking questions. Testing hypotheses.
Observing results. And sharing findings. The scientific community works
together in ways that few others do. Achieving the breakthroughs thatmight lead to cures or better therapies for cancer, Alzheimer’s disease,metabolic diseases, or any number of other human health issues
requires all of us to work hand in hand, as one.
We pledge to do our part. To provide you with the most reliable
products and services that our best minds can offer. To be yourcollaborative partner in your investigations, and be there for you
whenever you hit a roadblock. Our knowledge is your knowledge. Our
expertise is your expertise. Whatever the target of your investigation,
our mission is to continue to innovate, and to pioneer the developmentof new, specific, high-affinity reagents and other research tools
designed to help you advance your mechanistic studies and fuelbiological discovery.
We invite you to let us know how we can assist you in the importantresearch you’re doing. Whether you’re looking to explore new ways ofdeciphering cell activity, have a protocol question that our dedicatedteam can answer, want to download a signaling pathway, or just needa product shipped to you fast, we want to be your partner in doing
good science. As well as in doing good.

•15

Local Sales Representative

Doing good science is important.
So is just doing good.
Cell Signaling Technology (CST) is a different kind of life
sciences company—one founded, owned, and led by active
research scientists, with the highest standards of product andservice quality, technological innovation, and scientific rigor
for over 20 years. We consistently provide fellow scientists
around the globe with best-in-class products and services tofuel their quests for discovery.
Helping researchers find new solutions is our main mission
every day—but it’s not our only mission. We’re also dedicatedto helping identify solutions to other problems facing ourworld. We believe that all businesses must be responsible andwork in partnership with local communities, while seeking tominimize their environmental impact. That’s why we joined
1% for the Planet as its first life sciences member, and havecommitted to achieving net-zero emissions by 2029.
At CST, we believe in the power and promise of science tosolve the challenges we face as a global community. We’re acompany of caring people driven by a devotion to facilitating
good science—a company committed to doing the right thingfor our customers, our communities, and our planet.

cellsignal.com
CST® Antibody Performance Guarantee:
CST antibodies are guaranteed to work–the first time, and every time.
To learn more, please visit: cellsignal.com/abguarantee© 2023 Cell Signaling Technology, Inc. All rights reserved. Cell Signaling Technology, CST, PTMScan, XP, andHallmarks of Antibody Validation are trademarks or registered trademarks of Cell Signaling Technology, Inc.
CUT&RUN and CUT&Tag are licensed from Active Motif, Inc. under certain pending and granted patents.
For purchasers’ internal research use only. May not be used for resale, services, or other commercial use.
U.S. Patent No. 11,733,248, foreign equivalents, and child patents deriving therefrom. All other trademarks
are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
23-BCH-79930-EN-OCT23 For Research Use Only. Not for Use in Diagnostic Procedures.