JCB-SGT
A protein crystallization containerFeatures◆ Onboard space experiments: JCB-SGT has been used in the protein

crystallization project of JAXA. A 3-cell type with 3 cylindrical vessels
and a 6-cell type with 6 vessels on a 30 mm wide sheet are available.
◆ Various crystallization methods: Various crystallization methods, suchas batch, vapor diffusion, counter diffusion, dialysis, and diffusion pair osmotic concentration can be performed. It is useful for the optimizationof the conditions for space experiments and for ground-based control
experiments.
◆ Soft material: The JCB-SGT is made of PET sheet. By crushing thesilicon tube portion of the tubular sample container inside the JCB-SGT,
the diffusion can be suppressed. With releasing, crystallization can bestarted at the same timing as in space experiments.

6 cell type3 cell type

These products use theresults of technological◆ Heat seal: After filling the sample, it is heat-sealed. This prevents

development by JAXA

leakage of liquid completely and ensures high reproducibility andunder license.

reliability. Since the material is low gas permeability, water vapor
permeation from inside the container is minimized and the crystals arestable for more than one year.

Cat. ID

Product Name

Qty.

Price(w/o VAT)CRT420JCB-SGT 3 cell

2

¥19,000-

CRT421JCB-SGT 6 cell2¥38,000-

JCB-SGT LoadingTools
Point Sealer

Heat Controller Transformer

Cat. ID

Product Name

Qty.

Price(w/o VAT)

CRT300JCB-SGT Loading Tools(Point Sealer & HeatController)

1¥33,000-

CRT301

Transformer for 220V

1

Please contact us※ Please inquire for prices of space-standard products.

Available crystallization methods
Counter diffusion (CD): A capillary is filled with proteinsolution, a gel tube (CRT231-6) is attached and theother end is sealed with sealing compound. The capillary
is immersed in a crystallization reagent solution 1). Asingle capillary can screen a wide range ofcrystallization concentration conditions 2).
Dialysis (DL): After the sample loading, a gel tube
(CRT901-RC-6) with dialysis membrane is attached.
Others are the same as CD1).
Diffusion pair-osmotic concentration (DPOC): Fill aDPOC vessel (CRT850SP-6) with protein sample
solution and crystallization solution successively1).

SealingcompoundCapillary

Proteinsolution

Gel tubeCrystallizationsolution1) Takahashi S, et.al. Int. J. Microgravity Sci. Appl. (2019). 36(1), 360107.
https://doi.org/10.15011//jasma.36.360107.
2) Garcia-Ruiz, J.M. & Moreno, A.: Acta Cryst., D50, (1994) 484-490.
https://doi.org/10.1107/S0907444993014350.
To simulate the space experiment, C-Cap (CRT-413), gel tube with waterproof cover(CRT213-P-6), and DPOC vessel (CRT-850SP-6) are necessary.
For the optimization of crystallization conditions, C-Kit Ground Pro XRD (CRT101-1)
is available as an experimental kit for CD and DPOC methods.

JCB-SGT Set up procedure (CD method)
1. Fill each cell of the JCB-SGT with crystallization
solution up to ①. (It is easier to use the tool
made with a glass capillary connected to the tipof the pipettor tip via a silicon tube.)
2. Prepare the capillary assembly with the sample.
3. Load the assemblies into the cells of the JCBSGT, two for the 3-cell type and one for the 6cell large part. Normally, the gel tube side should
be down.
4. Add crystallization reagent to 1 mm below ②.
5. Heat-seal the upper side of ② while slightly
overflowing the crystallization solution. Be
careful not to leave any air in each cell.
6. Cut the cell with scissors at the position ③.
7. Heat seal the upper side of ②.
8. Place in an incubator to crystallize.

③②①※ Please inquire details on the setup method for space experiments.

Confocal Science Inc.
Musashino Bld. 2F Fukasawa 5-14-15,
Setagaya-ku Tokyo, 158-0081 Japan
TEL: +81-3-5809-1561 FAX: +81-3-6411-6261
E-mail : info@confsci.co.jp
Web: http://www.confsci.co.jp

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