CONTENTS
1. Immediate and delayed effects of postnatal administration of diprophos
Simona Clichici, Adriana Filip, Adela Joantă, C Puică
2. The effects of hemodialysis on some oxidative stress markers in chronic renal failure patients
Soimita Suciu, Simona Racasan, Adriana Muresan, Lavinia Sabau
3. Modulation of rat liver prooxidant/antioxidant balance by thyroid hormones
Adela Joantă, Adriana Filip, Simona Clichici, Şoimiţa Suciu, M Dorofteiu
4. Clinical significance and prognostic value of urinary low molecular weight (“tubular”) proteinsevaluated by SDS-polyacrylamide gel electrophoresis in glomerulonephritis
M Gherman-Caprioara, A Cristea, C Spanu, L Kacso
5. The effect of rehabilitation treatment after an uncomplicated acute myocardial infarction inpatients with or without type 2 diabetic nephropathy. Echocardiographic data
S Blaga, S Pop
6. Plasma levels of sE-selectin in patients with unstable angina pectoris
Luminiţa Vida-Simiti, Anca Cristea
7. Implications of erythropoietin in oxidative stress in hemodialysed patients suffering of chronic
renal failure
Irina Chis, Adriana Muresan
8. The involvement of free radicals in chronic venous insufficiency
Adriana Filip, Adriana Muresan, Adela Joanta, Simona Clichici, Gabriela Chereches, Doina
Daicoviciu, Nicoleta Decea
9. Book review: Textbook of algesiology
Francisc Schneider

1. IMMEDIATE AND DELAYED EFFECTS OF POSTNATAL ADMINISTRATION OF
DIPROPHOS
Simona Clichici1, Adriana Filip1, Adela Joanta1, C Puica2
1
Department of Physiology, University of Medicine and Pharmacy, Cluj-Napoca
2
Department of Biological Research, Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACT
Experimentally, we studied in rats the immediate and delayed effects of Diprophos administration(2µg/g body weight, i.m.) during the first 24 postnatal hours. Our results indicate the presence ofneuronal lesions 48 hours after the Diprophos administration, especially in the cerebral cortex andhippocampus, partially reversible until the age of 3 months, when the cerebral structures have analmost normal aspect, with the exception of mediobasal hypothalamus. Our results also indicate astatistically significant decrease of the learning capacity of conditioned reflexes of active avoidanceat the age of 3 months, demonstrated through the decrease of the positive reactions percentage andthe increase of the global time of latency average.
Key words: glucocorticoids, nervous system, Diprophos, learning, hippocampus, neurogenesis.

2. THE EFFECTS OF HEMODIALYSIS ON SOME OXIDATIVE STRESS MARKERS IN
CHRONIC RENAL FAILURE PATIENTS
Somita Suciu, Simona Racasan1, Adriana Muresan, Lavinia Sabau
Department of Physiology, University of Medicine and Pharmacy “Iuliu Hateganu” Cluj-Napoca
1“Mihai Manasia” Nephrology Clinic, Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACTIn this study we determined the plasma levels of thiobarbituric reactive substances (TBARS) andcarbonyl content of proteins, as well as the hydrogen donating ability, in 15 patients with chronic
renal failure undergoing hemodialysis, before and after one hemodialysis session. We found that the
average baseline levels of TBARS neither differ from those in controls, neither rose during
hemodialysis session. The carbonyl content of proteins did not show significant differences fromthose in controls, but increased significantly during hemodialysis session. Hydrogen donating ability
showed a large decrease during the hemodialysis session. The increase in the plasma levels ofcarbonyl content of protein as well as the decrease of hydrogen donating ability during one session ofhemodialysis suggest that hemodialysis per se may cause oxidative stress.
Key words: chronic renal failure, hemodyalisis, oxidative stress.

3. MODULATION OF RAT LIVER PROOXIDANT/ANTIOXIDANT BALANCE BY
THYROID HORMONES
Adela Joanta, Adriana Filip, Simona Clichici, Şoimita Suciu, M Dorofteiu
Physiology Department, University of Medicine and Pharmacy ”Iuliu Hatieganu” Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACTThe effects of the altered thyroid status on prooxidant/antioxidant balance of the rat’s liver were
examined. Hypothyroidism was induced by administering carbimazole (0,10 mg/100g body weight)
in drinking water for 14 days. Hyperthyroidism was elicited by a 7- day treatment of hypothyroid rats
with L-Thyroxine (10µg/100 g body weight). It has been determined from the rat’s liver theoxidative stress parameters such as thiobarbituric acid reactive substances (TBARS), protein
carbonyl content and the antioxidant enzymes activity (catalase, glutathion peroxidase,superoxiddismutase). The results of the present study indicated that administration of LThyroxine(T4) to hypothyroid rats resulted in significant augmentation of malondialdehyde(TBARS)
and protein carbonyl content of the liver comparative to euthyroid and hypothyroid rats. In
hypothyroid rats has been found a decrease of catalase activity, an increase of glutathione peroxidase and superoxiddismutase activity comparative to euthyroid rats. In hyperthyroid rats it has
been reported an increase of the antioxidant enzymes activity. The results of the present study
suggest that the liver prooxidant/antioxidant balance is considerably influenced by the thyroid statusof the body.
Key words: liver, oxidative stress, antioxidant enzymes.

4. CLINICAL SIGNIFICANCE AND PROGNOSTIC VALUE OF URINARY LOW
MOLECULAR WEIGHT (“TUBULAR”) PROTEINS EVALUATED BY SDSPOLYACRYLAMIDE GEL ELECTROPHORESIS IN GLOMERULONEPHRITIS
Gherman-Caprioara M1, A Cristea 2, C Spanu 1, I Kacso 1
1“Mihai Manasia” Nephrology Clinic,
2
Clinical Immunology Laboratory – 1st Medical Clinic
University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACTIn this study we characterized by SDS-PAGE the proteinuria of 220 patients with glomerulonephritis(GN) and 20 patients with microalbuminuria (diabetes mellitus,and essential hypertension).The

proteinuric patterns of a separate group of 35 patients with renal biopsy and diagnosed with primary
GN were correlated with the morphopathological lesions. The degree of irreversible tubulointerstitialdamage correlated with the low molecular weight proteins, which may have a predictive role for the
progression to CRF. The characterization of the proteinuria by SDS-PAGE may be a useful,noninvasive clinic tool for establishing prognosis in GN.
Key words: SDS-PAGE, progression to chronic renal failure, glomerulonephritis, proteinuric
patterns, low molecular weight proteins.

5. THE EFFECT OF REHABILITATION TREATMENT AFTER AN UNCOMPLICATEDACUTE MYOCARDIAL INFARCTION IN PATIENTS WITH OR WITHOUT TYPE 2
DIABETIC NEPHROPATHY. ECHOCARDIOGRAPHIC DATAS Blaga, S Pop
1st Medical Clinic – Internal Medicine and Cardiology,
University of Medicine and Pharmacy “Iuliu Haţieganu”, Cluj-Napoca, Romania
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACTThe paper studies the role of the rehabilitation treatment in type 2 diabetic patients with an acute
myocardial infarction, evaluated by the echocardiographic data.
We analyzed several echocardiographic parameters of the left ventricular function, in 40 type 2
diabetic patients, with of without diabetic nephropathy, after an acute myocardial infarction, who
underwent 6 weeks of rehabilitation program. The presence of the diabetic nephropathy has thesignificance of a poor prognosis in the rehabilitation period.
Key words: myocardial infarction, diabetic nephropathy, echocardiography, rehabilitation program.

6. PLASMA LEVELS OF sE-SELECTIN IN PATIENTS WITH UNSTABLE ANGINAPECTORIS
Luminita Vida-Simiti, Anca Cristea
1st Medical Clinic - Internal Medicine and Cardiology
University of Medicine and Pharmacy “Iuliu Haţieganu” Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACTIntroduction. Recent evidence suggests that inflammation plays a role in unstable angina pectoris(UAS). Leukocyte-endothelial cell interactions in inflammation are regulated by cell adhesion
molecules. E-selectin is a cell adhesion molecule that is only expressed on activated endothelium andis required for optimal leukocyte rolling and firm adhesion.
Aim of the study. The aim of the study was to evaluate the plasma levels of E-selectin in patientswith UAS in the first 5 days after the last spontaneous angina.
Methods. We measured sE-selectin (ELISA-sandwich) and CRP (nano-RID) in 9 patients with UAS
in the Braunwald class IIIB at 6, 12, 18 and 24 hours after the last angina, in 14 patients with UAS in
the class IIB at 5 days after de last angina and in 9 healthy volunteers (controls).
Results. As compared with the controls (4.8 ± 1.91ng/ml), in patients with UAS, plasma levels ofsE-selectin were significantly elevated in the first 6 hours after the last angina (25.91± 5.75 ng/ml)
and remained elevated at least 5 days (17. 64 ± 2.52ng/ml). The peak of the levels was recorded at 18
hours after the last angina. A negative correlation between sE-selectin and CRP was found.
Conclusion. In patients with UAS the demonstration of sE-selectin in the blood proves that theendothelial activation persists even in the clinically stabilized disease.

Key words: sE-selectin, CRP, unstable angina pectoris.

7. IMPLICATION OF ERYTHROPOIETIN IN OXIDATIVE STRESS IN HEMODIALYSEDPATIENTS SUFFERING OF CHRONIC RENAL FAILURE
Irina Chis, Adriana Muresan
Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACT
Even if aerobic metabolism provides advantages to different forms of life, oxygen as is both an
indispensable element for life and a source for very active detrimental and destructive potential
known under the name of reactive oxygen species (ROS). Its destructive effects on stimulated whenever it is an overproduction of ROS or when antioxidative defense systems on overtaken leading tooxidative stress.
Nowadays, the implications of ROS in different physiological and pathological processes are wellknown, some of them having relevance for clinical practice, as a causal factor, leading accompanying
or aggravating primary lesions. To sum up, it is perfectly justified the attempt to use biologicalagents with antioxidant effect in diseases involving ROS.
Erythropoietin (Epo) is an alpha 1-glicoprotein with an important antioxidant role intercepting
peroxide radicals and inhibiting lipid peroxidation amplification. In sources of ROS is chronic renal
failure and hemodialysis (HD) in patients with chronic renal failure.
Authors have looked for the protective role of Epo in oxidative stress in hemodialised patients andchronic renal failure. We determined the ROS in hemodialysis patients with chronic renal failure,through determination of lipid peroxides and proteins carbonyl; we also dosed antioxidative ferriccapacity, through determination of ceruloplasmin, an antioxidant which acts in extracellular tissue,
and donor capacity of H+, which shows the quantity of buffer substances from serum which is able toneutralize stable ROS after the patients were previously treated with recombinant humanerythropoietin (rHuEpo). The results were compared with the ones from a control group of healthy
persons.
Key words: erythropoietin, reactive oxygen species, protein carbonyl, lipid peroxides, hemodialysis.

8. THE INVOLVEMENT OF FREE RADICALS IN CHRONIC VENOUS INSUFFICIENCY
Adriana Filip1, Adriana Muresan1, Adela Joanta1, Simona Clichici1, Gabriela Chereches2, Doina
Daicoviciu2, Nicoleta Decea11
Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca
2Biochemistry, Immunology and Cellular Cultures Laboratory, Institute of Oncology Cluj-Napoca
Address for correspondence: Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, 13
Emil Isac Street, Cluj-Napoca, Romania

ABSTRACT
Chronic venous insufficiency is a frequent health problem, especially in females with a prolonged
evolution and important social costs.
There are a few pathogenetical hypothesis, but the hypothesis of white cell trapping with release freeoxygen radicals is the most actual.
This research aims to demonstrate the intervention of FOR through the dosage of parameters, whichquantify oxidant stress from blood, drawn from varicose veins and tissue homogenate from the veinwall. The values of lipid peroxides and carbonylate proteins were increased, demonstrating the local
production of free oxygen radicals (FOR) The local antioxidant defense was measured through thedetermination of the hydrogen donor capacity of a stable radical in the presence of an antioxidant

mix and ceruloplasmin. The results show that the inhibition of a free radical used is reduced inpatients with varicose illness.
Key words: chronic venous insufficiency, lipid peroxides, carbonylate proteins.

9. BOOK REVIEW: TEXTBOOK OF ALGESIOLOGY
Ostin C Mungiu
Edited by Ostin C Mungiu, Polirom Publishing House, 2002, ISBN 973-683-966-1
Professor Ostin C Mungiu, a prestigious Pharmacology Professor from Iasi, the initiator ofAlgesiology in Romania, coordinated an exceptional textbook regarding pain, including causes,
consequences and treatment.
Reviewing the 34 chapters, we realize the textbook touches every single domain where pain
generates sufferance and can be healed. It is signed by 43 authors of the most representative in the
medicine in Iasi and collaborators from Targu Mures and Bacau. Among authors are professors likeacademician Ion Haulica, Stefan Antohe, Mircea D Pavelescu, Eusebie Zbranca representing theclinical domain.
Pain, known in more then ten thousand diseases, is defined in the beginning of the book, followed byneuroanatomy, physiology, pharmacology and chronobiology. The methods of pain evaluation are
described next, pointing out pain as symptom in humans. The following chapters focus on opioid andnon-opioid analgesics and para-analgesics, local anesthetics and stress-induced analgesia. The mostrepresentative types of pain considering age, localization, symptomatic and causal therapy arereviewed.
The most important pain syndromes, such as angina pectoris, acute abdomen, pain in gynecology andobstetrics and rheumatic pain are exhaustively described. The next chapters concern central andperipheral neurological pain syndrome, migrainous and non-migrainous cephalalgia, etc. Special
chapters are dedicated to cancer pain and psychopathology of pain.
The last one hundred pages, out of 1036 pages of the book, present kinetotherapy, electrotherapy andplacebo therapy, ending with possible perspectives in pharmaco-analgesic strategies.
It is a captivating book, useful to those who serve the people in sufferance. Subsequently covering
this textbook we have to subscribe to the final conclusion of the editor “…as long as PAIN exists noeffort should be separated for its relief”.
Francisc Schneider