Drug absorption by skin treated by microplasma discharge
J. Kristof , T. Aoshima, M. G. Blajan and K. Shimizu

Shizuoka University, Nakaku, Johoku, Hamamatsu, 432-8561, Japane-mail: jaroslav.kristof@gmail.com, shimizu@cjr.shizuoka.ac.jp
APSPT-11, Kanazawa 11.-14. Dec. 2019

Introduction

ATR-FTIR characterization of the pig skin

Stratum corneum is first barrier that protect our body anddoes not allow penetration of amount of objects such as drugs.
Intercellular route is very often used for drug penetration
when stratum corneum lipids are extracted or their structureis changed. Change of properties of the skin surface canchange flow of transdermal drug delivery – if the drug is force
to adsorb on the skin surface.

Plasma treatment of the pig skin

Adenosine: hydrophilic drug
For treatment of abnormally fastheart rhythmwith molecular weight of 267 Da

Invetigation of the skinabsorption ofGalantamine Hydrobromide:hydrophilic drugfor Alzheimer disease
with molecular weight of 287 Da

ATR-FTIR analysis

Infrared lipid bands simulation

Symmetric CH3
Asymmetric CH3
Asymmetric CH2Symmetric CH2

Cyclosporine A:
Lipophilic drug
As imonosupressive drug during
and after transplantation of organs
with molecular weight of 267

Etching effect of the microplasma treatmentof the pig skin
Stratum corneum
Thickness = 30 μm

Increase of thebandwidth andwavenumber shift
indicate disturbationof lipid structure

Stratum corneum
Thickness = 14 μm

200 μmHigh Performance liquid Chromatography (HPLC)

200μm

drug

HPLC analysis
40
39

38

38

37

37

36

36

35

35

34

34

33

33

32

32

31

3130

30

29

29
030

60

90

120000Adhesive tape strip

60000
40000
20000Absorption and permeability of GaHBr

1010.1

microplasma

control

140000120000aborbance (a.u.)Stratum spinosum

Conclusions

controlabsorbed
permeated

Stratum corneum
(SC)

04.4
7.25
9.75
12.25
Stratum corneum density ρ= 1g/cm3
depth in µm
Surface areaS = (1.5 x 1.5) cm2
Tape
strippingallow
astoRemoved thickness
d = m/(ρ S)
analyze depth of influence ofthe microplasma

microplasma

100

Stratum granulosum

Absorption of Cycloporine A
After plasma treatment, absorption of the skinfor Cyclosporine A increased about 32%

800000time [s]absorbed

100000120 150 180 210 240 270 300

Tape tripping test

HPLC analysis

Skin permeability/absorption
140000aborbance (a.u.)

DBD microplasma

39aborbance (a.u.)

Skin temperature is under 45°C –No temperature damage of the skin40

temperature of skin during
treatment [°C]

Ar microplasma discharge

temperature of skin during
treatment [°C]

Temperature of plasma treated skin

Skin soaked indrug solvent
For 24 hour

Absorption of Adenosine

absorbed

100000
80000
60000
40000
20000
0

After plasma treatment, absorption of the skinfor GaHBr was comparable with non-treatedskin but permeability increased significantly

microplasma■ Microplasma irradiation can etch the surface of the skin
■ Plama temperature allows safe skin treatment■ Microplasma diturb structure of the skin lipids (ATR-FTIR meaurements)■ Microplasma can improve drug absorption of the skin or permeability of the skincontrol

After plasma treatment,
absorption of the skin for
GaHBr was comparablewith non-treated skin
but permeability
increased significantly