17IPB_ques.txt★★★ ＜第１７回知的財産翻訳検定試験【第８回英文和訳】＞ ★★★
≪１級課題 -バイオテクノロジー-≫【解答にあたっての注意】
１．問題の指示により和訳してください。
２．解答語数に特に制限はありません。適切な個所で改行してください。
３．課題文に段落番号がある場合、これを訳文に記載してください。
４．課題は４題あります。それぞれの課題の指示に従い、４題すべて解答してください。
問１．***START***から***END***までを和訳してください。
(WO2013149323)
NATURAL PRODUCTS FOR SKIN CARE***START***
Essentially, there exist several skin types:
Combination skin: This skin type is very common and requires separate
treatment for each area. It is most generally oily down the T-zone of thefacial area and dry on the cheeks.
Dry Skin: This type of skin lacks both sebum and moisture. It looks finetextured, transparent, patchy and fragile. The skin flakes and chaps moreeasily compared to other skin types, especially after cleansing, where it
feels rough, dry and tight. Skin looks dull.
Oily Skin: This skin type has over-reactive sebaceous (oil-producing) glands,
which makes the face shiny especially down the central panel (T-zone) of yournose, forehead and chin.
Sensitive Skin: This skin type is prone to redness and is reactive. It often
feels tight, hot, rough, or itchy in some areas. It reacts easily toenvironmental factors, such as the cold, the wind, pollution, and the like.
Normal Skin: This skin type has a balanced oil and water content and it feels
smooth and velvety. The color of the skin glows below the translucent surface.
This skin type definitely needs care if it is to last.
***END***
問２．***START***から***END***までを和訳してください。
(EP2322561)
ANTI-NIK ANTIBODIES AND USES THEREOF***START***
Purifying the antibody or antibody fragment capable of binding thetarget antigen with a desired affinity from a preparation of the present
invention, such as an unpurified anti-serum of the present invention, can beachieved, for example, via affinity chromatography purification of an
unpurified or more preferably a protein-G purified-anti-serum of the presentinvention, by using the target antigen as an affinity ligand, and via
selective elution of a substrate-bound antibody or antibody fragment underconditions of controlled stringency (for example under conditions ofcontrolled pH and/or salt concentration). In particular, an antibody orantibody fragment of the present invention capable of binding the target
ページ(1)

17IPB_ques.txt
antigen with a maximal affinity may be conveniently obtained by elution underconditions of effectively maximal stringency (for example under conditions ofeffectively maximal or minimal pH and/or maximal salt concentration).
Typically,an antibody or antibody fragment may be bound to a
substrate-attached cognate antigen thereof under conditions of physiologicalpH and salt concentration, and such an antibody or antibody fragment may
typically be eluted from the substrate by decreasing the pH to 2.5 or lower,
or by increasing the pH toll or higher.
***END***問３．***START***から***END***までを和訳してください。
(WO2013151650)
NEUROPHILIC NANOPARTICLES***START***
Primary Schwann cell cultures were established from newborn rat pups.
Briefly, sciatic nerves can be dissociated enzymatically and mechanically andplated in normal growth medium. From one neonatal rat litter enough cells wereobtained for up to six experiments, each providing multiple 6-cm and two24-well plates. Since the yields from rat samples are much higher as comparedto mouse, initial experiments were performed in rat cells. Based on
preliminary studies, DOPC/P188 liposomes compositions that are taken up by ratcells (Fig. 1) are similarly effective in cultures from mice (Fig. 5), andDOPC/P188/Cholesterol liposome compositions that are taken up by rat cells(Fig. 3) are similarly effective in cultures from mice (Fig. 5). The purity of
the Schwann cell cultures was routinely evaluated by immunolabelling and wasover about 90% as judged from anti-p75 and anti-S100 reactivity.
Dissociated dorsal root ganglion (DRG) neuron cultures wereestablished from embryonic day 15 (E15) rodents. These non-myelinating,dissociated cultures can serve to assess the uptake of liposomes into sensory
neurons.
***END***
問４．***START***から***END***までを和訳してください。
(WO2013138684)
GENETICALLY ENCODED FLUORESVENT SENSORS FOR DETECTING INTRACELLULAR SIGNALING
THROUGH DIACYLGLYCEROL PATHWAYS***START***
What is claimed is:
1. A diacylglycerol (DAG) sensor fusion protein comprisinga. a PKC protein comprising a DAG binding domatin and a fusion region,andb. a circularly permuted fluorescent protein,
wherein the fusion region is located upstream from the DAG bindinh
domain or within the DAG binding domain;
wherein the fluorescent protein is fused with the PKC protein at aページ(2)17IPB_ques.txt
fusion site present within the fusion region;
and wherein the fluorescene of the DAG sensor fusion protein changes
upon binding to DAG.
14. A multiplex system for detecting one or more analytes comprising the DAG
sensor fusion protein of any one of claims 1-13, and one or more addisional
fluorescent sensor that specifically detects and analyte other than DAG,
wherein the fluorescent sensor comprises a) a fluoerscent sensor fusion
protein comprising a fluorescent protein, or b) a fluorescent dye.
***END***

ページ(3)