Simple and Sensitive COVID-19 Detection

Quick SARS-CoV-2 Multiplex Kit

Testing for COVID-19 Detection

The Quick SARS-CoV-2 Multiplex Kit is a real time reverse transcription PCR (rRT-PCR) test for the qualitative
detection of RNA from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is responsible for
COVID-19. The Quick SARS-CoV-2 Multiplex Kit detects a SARS-CoV-2 specific target from the nucleocapsid (N)gene and a host-specific target from the human RNase P gene.

Highlights
Top PerformanceCertifications

SimpleWith an LoD of only 167 GEC/ml,
the Quick SARS-CoV-2 Multiplex
Kit is one of the most sensitive
molecular tests on the market.

The Quick SARS-CoV-2 Multiplex
Kit is CE IVD marked for in vitro
diagnostic use and in submission
for FDA EUA.

With a ready-to-use master mix
that detects both SARS-CoV-2
and an internal host control, theQuick SARS-CoV-2 Multiplex Kit
minimizes hands-on time.

Competitive Pricing

Flexible

Robust Controls

We are committed to offering theQuick SARS-CoV-2 Multiplex Kit
at competitive pricing to enable
low cost testing for COVID-19.

The Quick SARS-CoV-2 Multiplex
Kit is compatible with Real Time
PCR instruments capable ofdetecting HEX/VIC and Quasar
670/Cy5 fluorophores.

The controls included in the kit
enable the easy identification ofproblems that may arise during
specimen collection, nucleic acidextraction, and RT-PCR.

Kit Components
No Template Control

This control is extracted in parallelwith clinical samples and is used toidentify potential contamination of
extraction and RT-PCR reagents.

CV Positive Control

2X CV Mix

Detects one viral target located in theSARS-CoV-2 N gene (detected in theHEX/VIC channel) and a target for human
RNase P (detected by the Quasar 670/Cy5channel). Detection of RNase P serves asa quality control for specimen collectionand nucleic acid extraction.

The positive control simulates a low positive patient sample and contains 2.5 GEC/µl of a SARS-CoV-2
RNA fragment (25 GEC/rxn) and 1 ng/ul of human RNA (10 ng/rxn). This control is added directly to theRT-PCR plate to ensure that all components of the kit are working properly.

Specifications
Extracted RNA samples are analyzed using the CFX96 Touch Real-Time PCR System (Bio-Rad) or a RT-PCR instrument
capable of detecting HEX/VIC and Quasar 670/Cy5 fluorophores.

RT-PCR Set-up

Data Analysis & Interpretation
Interpret the results based on Ct values

Component

Volume per Reaction

Host Target

Viral Target

Results

2X CV Mix
Template RNA

10 µl
10 µl+-SARS-CoV-2 Negative++SARS-CoV-2 PositiveTotal

20 µl-+SARS-CoV-2 Positive--Invalid

Performance:• PPA: 30/30 = 100% (95% CI: 88.7% - 100%)• NPA: 30/30 = 100% (95% CI: 88.7% - 100%)• 100% accurate detection as low as 10 GEC/rxn

Percent of Positive
Samples Detected

Select HEX/VIX channel for the detection of SARS-CoV-2 target
and Quasar 670/Cy5 for the detection of human target.

100%
85%

85%

2.55SARS-CoV-2 GEC/reaction

Ordering Information
Catalog Number

Product Name

Size

R1106
R1107

DNA/RNA Shield™ Collection Tube w/ Swab (1 ml fill)

10 pack
50 pack

R1108
R1109

DNA/RNA Shield™ Collection Tube w/ Swab (2 ml fill)

10 pack
50 pack

R1210

DNA/RNA Shield™ Saliva/Sputum Collection Kit

1 x 2 ml fillSample Purification

R2140
R2141Quick-DNA/RNA™ Viral MagBead Kit

1 x 96 preps
4 x 96 preps

SARS-CoV-2 Detection

R3013

Quick SARS-CoV-2 Multiplex Kit

100 tests per kit
1,000 tests per kit
10,000 tests per kit

Sample Collection

The test has been not FDA cleared, approved, or authorized.

10Cat. No. B1017-2-02

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info@zymoresearch.com

www.zymoresearch.com

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