SUPER-RESOLUTION MICROSCOPY WORKSTATION

Vutara VXLSingle-Molecule Localization for Advanced Biological Imaging

Vutara VXL
See biology in nanoscale detail
The Vutara VXL comprehensive biologicalworkstation enables both core facilities
and individual investigators to easily
incorporate super-resolution microscopy
in their research. This system utilizes
single-molecule localization microscopy(SMLM) technology to achieve the highest
resolution of all super-resolution techniques. When
combined with Bruker’s microfluidics unit, Vutara VXL enables multiplexedimaging for targeted spatial genomics, transcriptomics, and proteomics
research. With advanced SRX software, researchers can transform complex
data into meaningful information to explore new frontiers in disciplines
ranging from neuroscience and cell biology to virology and more.
The Vutara VXL advantage: SMLM approach delivers the highest
resolution, down to 20 nm laterally Proprietary Biplane™ technology achieves
deepest 3D imaging, with depths ofup to 50 μm
 Innovative top-hat illumination enables
uniform, quantitative data collection“Having published with a Vutara super-resolution system, I can confidently say that they offer
one of the most advanced super-resolution microscopes available. Their attention to detail andongoing close collaboration makes them one my preferred microscopy vendors– Bridgette Ritter, Ph.D., Boston University”

Leading Super-Resolution Technology
Our Approach: Single-Molecule Localization
Bruker has been a leader in super-resolution technology for over a decade, offering the highest quality and mostadvanced technology on the market. Vutara VXL utilizes single-molecule localization (SML), providing the highestresolution compared to other super-resolution approaches, down to 20 nm laterally. To overcome the optical resolution
limit with SMLM, only a limited number of chromophores are activated at a time so that their point-spread-functions(PSF) do not overlap. The microscope detects these chromophores, determines their location, and switches them off.
Then another subset of chromophores is activated, localized, and deactivated. This cycle is repeated multiple times tocreate a high resolution localization map.
Stochastic excitation of molecules can be achieved by either the use of fluorescently labeled proteins or organic
fluorescent dyes. Vutara VXL is fully compatible with common and emerging SMLM techniques, including: Photoactivated localization microscopy (PALM)
 Stochastic optical reconstruction microscopy (STORM) and direct-STORM (dSTORM)
 Points accumulation for imaging in nanoscale topography (PAINT) and DNA-PAINT

Innovation for Unlimited Potential
Developed in collaboration with leaders in industry and academia, Vutara VXL pushes the boundaries of standard
super-resolution capabilities. Proprietary biplane technology, combined with a spatial filter in the emission lightpath, allows you to acquire 3D data up to 50 μm deep into the sample. The system allows researchers to easily
perform a Z series and automatically localizes and reconstructs the entire volume. Additionally, the utilization of
top-hat illumination results in uniform data collection across the entire imaging region, enabling reliable quantitative
data analysis. Vutara VXL was designed to be a comprehensive biological workstation, tailored to the needs ofmicroscopy experiments from start to finish. The imaging ecosystem—including the Vutara VXL microscope, themicrofluidics unit, and the SRX software—supports seamless acquisition and analysis of rich, quantitative data.
Background image:Actin-magenta, microtubule-yellow, mitochondria-orange“

With more than 15 years of experience in designing microscopes in academia and in indutry, I am proud
to contriubute to the development of Vutara's super-resolution microscopes. With Biplane 3D detection”

and fast sCMOS imaging, Vutara has the most advanced super-resolution microscope on the market.
– Joerg Bewersdorf, Ph.D., Yale University

Enabling Advanced Research
Across the Life Sciences
Vutara VXL enables research on DNA, RNA, and proteins, from macromolecular complexes and super-structures tochromatin structure and chromosomal substructures, to studying functional relationships
in genomes and in various subcellular organelles. This novel system also supports
advanced spatial biology research in extracellular matrix structures, extracellular
vesicles, virology, neuroscience, and live-cell imaging. When combined with
Bruker’s unique microscope fluidics unit, Vutara VXL supports multiplexed
imaging for targeted, submicrometer multiomics in genomics,
The synaptic labelling of C. elegans.
transcriptomics, and proteomics research.
Three markers present in this image:yellow (Skylan-s Active zone marker),
magenta (HaloTag::JF646 Calcium
Channel 1) and cyan (SNAPf::JF549
Calcium Channel 2).
Image courtesy of Dr. Sean Merrill and Dr. Erik
Jorgensen, University of Utah.

Neuroscience
Imaging the brain in nanoscale detail beyond the optical
diffraction limit opens doors in neuroscience research. Perhaps
one of the most interesting components of the brain is thesynapse—the junction essential to the transmission ofsignals in the brain. Super-resolution microscopy
is required to visualize these structures thatare approximately 20-40 nm wide. The SML
method is ideal for the study of neuronalstructures and processes such as:Neural development

 Neural circuit assembly Synaptic formationSynaptic transmission
Synaptic proteins

Genomics
Visualizing genomicorganization and structure
on a subchromosomal level is necessary forunderstanding relationships between genes,
their regulation, and their environment.
SMLM can image three-dimensional
chromosome structures at single celland subcellular levels, making it ideal for
genomics applications such as: Functional organization of genome
within the nucleus Multi-location capture ofmultiple nuclei
 Chromatin tracingChromosome 19 topologically associating domain(TAD, in magenta) within a compartment (in blue).
Image courtesy of Ting Wu Lab, Harvard University.

Virology
The Vutara SMLM platform has been a critical
instrument for understanding viral particles.
Viral particles are typically much smaller thanthe optical diffraction limit, making SMLM
the best suited fluorescence techniquefor resolving virus particle structuraldetails or determining localization ofvirus components with the cellularmachinery. Some key applications
for Vutara VXL in virology research
include: Viral particle structure Virus-host interactions
 Virus pathology

Cell Biology
Many interesting cellular structuresare smaller than the optical
diffraction limit of ~200-300 nm andtherefore require super-resolution
microscopy for imaging specificallylabeled structures at this scale. These
include the substructures of most organelles
and all macromolecular machines, channels, andreceptors. Research applications in cell biologythat can be achieved with Vutara VXL include: Molecular quantification
 Molecular distribution
 Colocalization of proteins Particle tracking

Vesicular stomatitis viral particle. Red – VSV-G protein
tagged with Alexa Fluor 647.

Mitochondria stained for TOM20 with
Alexa 647, imaged with SMLM (dSTORM).

Transforming Voxels into Information
Rich Quantitative Analysis
Capabilities
With SRX software and its quantitativelocalization microscopy analysis suite,
Vutara VXL can provide both visual andquantitative information from biologicalsamples. By localizing individual molecules,
Vutara can generate stunning 3D images
while simultaneously providing in-depth
quantitative analysis tools.

Measurements available within theSRX software include: Spatial Distribution — Provides avariety of tools for analyzing spatial
distribution relationships of particles,including Ripley’s K, pair correlation, andnearest neighbors
 Cluster Analysis — Counts clusters,cluster sizes, and cluster densities
 Colocalization — Provides statisticalmeasures on relationships between
particles of two different labels
 Resolution Analysis — Quantifies
resolution for images derived fromlocalized data
 Live-Cell Analysis — Tracks clusters
in live-cell experiments with meansquared displacement and angulardisplacement analyses“The workflow-oriented SRX software includes powerfulstatistical analysis tools.

After scanning the market for super-resolution microscopes and personally visiting and testing mostcommercially available systems with our own samples, I can say that I am most impressed with the Vutara.
In particular, the user friendliness of their imaging software as well as the 3D capability in super-resolution
mode impressed me. Vutara has an excellent support team and staff as a whole making our transition tosuper-resolution a well-supported experience. If you're looking to advance your research by super-resolution
microscopy, I can confidently say that Vutara's systems are an excellent choice.

– Thomas Stroh, Ph.D., Core Facility Directory, McGill University.

”

Multiplexing for
Unlimited Imaging Possibilities
Bruker’s Microfluidics Accessory
The microfluidics unit is specificallydesigned to allow sequential labelling to beincorporated into localization microscopy
protocols with the Vutara VXL microscope.
The unit is controlled by SRX softwareand allows flexible configuration of fluidics
sequences that are run in conjunction withimaging cycles. This enables users tolabel an unlimited number of probes andimage them in a sequential fashion.
With the microfluidics unit, Vutara VXL
can be used to address any localization
microscopy application that requires alarge number of targets to be imaged.
Fifteen probe vials are provided and canbe interchanged during experiments, thus
supporting an unlimited number of targets.
Primary localization microscopy applications
with the microfluidics unit include: OligoSTORM
 RefreshSTORM
 DNA-PAINT Multiplexed antibody labellingSMLM with high Z-resolution for STORM, PALM, PAINT, and related super-resolution applications;
Imaging Modalities

Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications;Epi-fluorescence microscopy with large FOV;
Transmitted light microscopy with large FOV
405 nm, 120 mW

Excitation lasers(nominal laser power at diode)488 nm, 2000 mW (optional)
555 nm, 2000 mW
638 nm, 1200 mW
750 nm, 1500 mW (optional)

Flat Illumination

Flat excitation profile guaranteed by top-hat illumination from a square fiber

Multi-Color Acquisition≤5 colors sequential

Multi-Plane Imaging

Simultaneous imaging of two focal planes allows 1 µm depth discrimination (larger Z range possible in Z-stack mode)

CameraOrca Flash 4.0 v3 sCMOS camera; Orca Fusion BT sCMOS camera (optional)

Objective
Field of View (FOV)

60x magnification; 1.3 NA; Silicon oil immersion; 0.3 mm working distance;#1.5H cover glass (0.170 ±0.005 mm)
200 x 200 µm for multiplexed localization microscopy and widefield imaging; 50 x 50 µm for SMLM with switching(STORM, PALM, PAINT) and 3D localization (Biplane detection); Larger FOV with tile scanning

SMLM Resolution

20 nm laterally (XY); 50 nm axially (Z) with BiplaneImaging Depth>30 µm (dependent on sample)

XY-Stage

Easy access to sample; 100 x 50 mm travel range; Optical encoder with 1 nm resolution

Z-Focus

Course focus to localize sample; Fine focus for fast Z-stack acquisition

Drift Correction
Workflow-Oriented Software
Options

Active focus drift correction during data acquisition; Focus drift <30 nm over 10 minutes;
XY drift correction post-acquisition
Data acquisition; 3D localization based on measured PSF; Particle tracking; Statistical data analysis tools; Visualization
Integrated microfluidics for multiplexed experiments; Live-cell incubation with humidity, CO2, and temperature
control; Network attached storage

Bruker Fluorescence Microscopy | See the Biology of Life More Clearly
Madison, WI • USA
Phone +1.608.662.0022
productinfo@bruker.com

www.bruker.com/Vutara-VXL

All specifications are typical. Bruker is continually improving its products and reserves the right to change specifications
without notice. Biplane, SRX, and Vutara VXL are trademarks of Bruker Corporation. All other trademarks are the propertyof their respective companies. © 2021 Bruker Corporation. All rights reserved. B2009, Rev. A0.

Specifications