Powerful platform enabled by NMR

Nightingale
Blood
Biomarker
Analysis
Service

Unique combination of biomarkers

Comprehensive overview of an individual’s health

Biomarkers include clinically validated routine markers, emerging biomarkers with strong medical
relevance and promising, new biomarkers.

Biomarkers in our panel provide a physiologicallymeaningful picture of the overall health making it
possible to explore novel connections between
metabolites and an individual’s health status.

Robust and highly reproducible results
Our fully automated analysis process is constantly
monitored under a certified quality management
system. And the NMR technology allows highreproducibility which ensures consistent andreliable results across all sample sets.

Nightingale’s quality management system has beencertified according to EN ISO 13485 standard.

TECH SPECIFICATIONS

Fast, cost-efficient and scalable technology
We use a high-throughput NMR technology which
ensures efficient analysis for sample sets of all sizes
without batch effects.

Technology

1Specimen type

Serum and Plasma

Sample volume

100 µL and 350 µL

H NMR Spectroscopy,
Nightingale Health’s
proprietary analysis

Accurate and fully quantified metabolic data

Number of biomarkers

250

Not only our analysis, but even our quantification
process of the NMR spectral data is fully automated,which provides precise and accurate metabolite
results in absolute concentration units.

Result units

Absolute biomarker
quantification (mmol/l or g/l)

Sample storage

Long-term storage-70˚C or below

CLINICALLY AND ANALYTICALLY VALIDATED BIOMARKERS (37)

Cholesterol

Boost your researchwith comprehensive blood
metabolite insights.

Certified quality management system

Total cholesterol
VLDL cholesterol
Clinical LDL cholesterol
HDL cholesterol

mmol/lmmol/lmmol/lmmol/lTriglycerides
Total triglycerides

mmol/lFatty acids
Total fatty acids
Omega-3 fatty acids
Omega-6 fatty acids
Polyunsaturated fatty acids
Monounsaturated fatty acids
Saturated fatty acids
Docosahexaenoic acidmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lFatty acid ratios
Ratio of omega-3 fatty acids
to total fatty acids
Ratio of omega-6 fatty acids tototal fatty acids%%

Ratio of polyunsaturated fatty
acids to total fatty acids
Ratio of monounsaturated fatty
acids to total fatty acids
Ratio of saturated fatty acids tototal fatty acids
Ratio of docosahexaenoic acidto total fatty acids
Ratio of polyunsaturated fatty acids
to monounsaturated fatty acids
Ratio of omega-6 fatty acids
to omega-3 fatty acids

%
%
%
%

Aromatic amino acids%

Phenylalanine
Tyrosine

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lGlycolysis related metabolites
g/l
g/l
ratio

Amino acids
AlanineGlycineHistidine

Total concentration of
branched-chain amino acids(leucine + isoleucine + valine)
Isoleucine
LeucineValine%Apolipoproteins
Apolipoprotein B
Apolipoprotein A1
Ratio of apolipoprotein B
to apolipoprotein A1

Branched-chain amino acids

mmol/lmmol/lmmol/lGlucose
Lactatemmol/lmmol/lFluid balance
Creatinine
Albuminmmol/lg/lInflammation
Glycoprotein acetyls

mmol/lList of all biomarkers

Cholesterol
Total cholesterol
Total cholesterol minus HDL-C
Remnant cholesterol(non-HDL, non-LDL -cholesterol)
VLDL cholesterol
Clinical LDL cholesterol
LDL cholesterol
HDL cholesterol

Other lipids
mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lTriglycerides
Total triglycerides
Triglycerides in VLDL
Triglycerides in LDL
Triglycerides in HDL

mmol/lmmol/lmmol/lmmol/lPhospholipids
Total phospholipids in lipoprotein particles
Phospholipids in VLDL
Phospholipids in LDL
Phospholipids in HDL

mmol/lmmol/lmmol/lmmol/lCholesteryl esters
Total esterified cholesterol
Cholesterol esters in VLDL
Cholesterol esters in LDL
Cholesterol esters in HDL

mmol/lmmol/lmmol/lmmol/lFree cholesterol
Total free cholesterol
Free cholesterol in VLDLFree cholesterol in LDLFree cholesterol in HDL

mmol/lmmol/lmmol/lmmol/lTotal lipids
Total lipids in lipoprotein particles
Total lipids in VLDL
Total lipids in LDL
Total lipids in HDL

Apolipoprotein B
Apolipoprotein A1
Ratio of apolipoprotein B to apolipoprotein A1

Total fatty acids
Degree of unsaturation
Omega-3 fatty acids
Omega-6 fatty acids
Polyunsaturated fatty acids
Monounsaturated fatty acids
Saturated fatty acids
Linoleic acid
Docosahexaenoic acidmmol/lmmol/lmmol/lmmol/lBranched-chain amino acids

Average diameter for VLDL particles
Average diameter for LDL particles
Average diameter for HDL particles

All listed biomarkers are available for Serum and Heparin plasma samples.

nm
nm
nm

mmol/ldegreemmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lFatty acid ratios
Ratio of omega-3 fatty acids to total fatty acids
Ratio of omega-6 fatty acids to total fatty acids
Ratio of polyunsaturated fatty acids to total fatty acids
Ratio of monounsaturated fatty acids to total fatty acids
Ratio of saturated fatty acids to total fatty acids
Ratio of linoleic acid to total fatty acids
Ratio of docosahexaenoic acid to total fatty acids
Ratio of polyunsaturated fatty acids
to monounsaturated fatty acids
Ratio of omega-6 fatty acids to omega-3 fatty acids

AlanineGlutamine
GlycineHistidine

Total concentration of branded -chain aminoacids (leucine + isoleucine + valine)
Isoleucine
LeucineValine

%
%
%
%
%
%
%
ratio
ratio

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lBiomarkers marked with * are not available for EDTA plasma samples.
Biomarkers marked with ** are not available for Citrate plasma samples.

Medium VLDL (average diameter 44.5 nm)
mmol/lmmol/lmmol/lmmol/lmmol/l3-Hydroxybutyrate
AcetateAcetoacetate
Acetonemmol/lmmol/lmmol/lmmol/lFluid balance
Creatinine
Albuminmmol/lg/lInflammation
Glycoprotein acetyls

mmol/l

Lipoprotein subclasses
Chylomicrons and extremely large VLDL
(particle diameters from 75 nm upwards)
Concentration of chylomicrons andextremely large VLDL particles
Total lipids in chylomicrons andextremely large VLDL
Phospholipids in chylomicrons andextremely large VLDL
Cholesterol in chylomicrons andextremely large VLDL
Cholesteryl esters in chylomicrons andextremely large VLDLFree cholesterol in chylomicrons andextremely large VLDL
Triglycerides in chylomicrons andextremely large VLDL

mmol/lmmol/lmmol/lmmol/lmmol/lConcentration of large VLDL particles
Total lipids in large VLDL
Phospholipids in large VLDL
Cholesterol in large VLDL
Cholesteryl esters in large VLDLFree cholesterol in large VLDL
Triglycerides in large VLDL

Concentration of small VLDL particles
Total lipids in small VLDL
Phospholipids in small VLDL
Cholesterol in small VLDL
Cholesteryl esters in small VLDLFree cholesterol in small VLDL
Triglycerides in small VLDL
Concentration of very small VLDL particles
Total lipids in very small VLDL
Phospholipids in very small VLDL
Cholesterol in very small VLDL
Cholesteryl esters in very small VLDLFree cholesterol in very small VLDL
Triglycerides in very small VLDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lIDL (average diameter 28.6 nm)

Large LDL (average diameter 25.5 nm)

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lVery small VLDL (average diameter 31.3 nm)

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lSmall VLDL (average diameter 36.8 nm)

mmol/lmmol/lVery large VLDL (average diameter 64 nm)
Concentration of very large VLDL particles
Total lipids in very large VLDL
Phospholipids in very large VLDL
Cholesterol in very large VLDL
Cholesteryl esters in very large VLDLFree cholesterol in very large VLDL
Triglycerides in very large VLDL

Concentration of medium VLDL particles
Total lipids in medium VLDL
Phospholipids in medium VLDL
Cholesterol in medium VLDL
Cholesteryl esters in medium VLDLFree cholesterol in medium VLDL
Triglycerides in medium VLDL

Concentration of IDL particles
Total lipids in IDL
Phospholipids in IDL
Cholesterol in IDL
Cholesteryl esters in IDLFree cholesterol in IDL
Triglycerides in IDL

mmol/lLarge VLDL (average diameter 53.6 nm)

Aromatic amino acids
Phenylalanine
Tyrosine

Glucose
LactatePyruvate
Citrate **Glycerol *Ketone bodies
g/l
g/l
ratio

Fatty acids

Amino acids

Lipoprotein particle sizes

Glycolysis related metabolites
mmol/lratio
mmol/lmmol/lmmol/lApolipoproteins

mmol/lmmol/lmmol/lmmol/l

Lipoprotein particle concentrations
Total concentration of lipoprotein particles
Concentration of VLDL particles
Concentration of LDL particles
Concentration of HDL particles

Phosphoglycerides
Ratio of triglycerides to phosphoglycerides
Total cholines
Phosphatidylcholines
Sphingomyelins

Concentration of large LDL particles
Total lipids in large LDL
Phospholipids in large LDL
Cholesterol in large LDL
Cholesteryl esters in large LDL
Free cholesterol in large LDL
Triglycerides in large LDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lMedium LDL (average diameter 23 nm)
Concentration of medium LDL particles
Total lipids in medium LDL
Phospholipids in medium LDL
Cholesterol in medium LDL
Cholesteryl esters in medium LDL
Free cholesterol in medium LDL
Triglycerides in medium LDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lLB-0428 2/2020

List of all biomarkers

Small LDL (average diameter 18.7 nm)
Concentration of small LDL particles
Total lipids in small LDL
Phospholipids in small LDL
Cholesterol in small LDL
Cholesteryl esters in small LDL
Free cholesterol in small LDL
Triglycerides in small LDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lVery large HDL (average diameter 14.3 nm)
Concentration of very large HDL particles
Total lipids in very large HDL
Phospholipids in very large HDL
Cholesterol in very large HDL
Cholesteryl esters in very large HDL
Free cholesterol in very large HDL
Triglycerides in very large HDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lLarge HDL (average diameter 12.1 nm)
Concentration of large HDL particles
Total lipids in large HDL
Phospholipids in large HDL
Cholesterol in large HDL
Cholesteryl esters in large HDL
Free cholesterol in large HDL
Triglycerides in large HDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lMedium HDL (average diameter 10.9 nm)
Concentration of medium HDL particles
Total lipids in medium HDL
Phospholipids in medium HDL
Cholesterol in medium HDL
Cholesteryl esters in medium HDL
Free cholesterol in medium HDL
Triglycerides in medium HDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lSmall HDL (average diameter 8.7 nm)
Concentration of small HDL particles
Total lipids in small HDL
Phospholipids in small HDL
Cholesterol in small HDL
Cholesteryl esters in small HDL
Free cholesterol in small HDL
Triglycerides in small HDL

mmol/lmmol/lmmol/lmmol/lmmol/lmmol/lmmol/lCholesteryl esters to total lipids ratio in medium LDL
Free cholesterol to total lipids ratio in medium LDL
Triglycerides to total lipids ratio in medium LDL

%
%
%
%
%

Small LDL ratios

%
%
%
%
%

Very large HDL ratios

%
%
%
%
%

Large HDL ratios

%
%
%
%
%

Medium HDL ratios

%
%
%
%
%

Small HDL ratios

Very large VLDL ratios
Phospholipids to total lipids ratio in very large VLDL
Cholesterol to total lipids ratio in very large VLDL
Cholesteryl esters to total lipids ratio in very large VLDLFree cholesterol to total lipids ratio in very large VLDL
Triglycerides to total lipids ratio in very large VLDL

Large VLDL ratios
Phospholipids to total lipids ratio in large VLDL
Cholesterol to total lipids ratio in large VLDL
Cholesteryl esters to total lipids ratio in large VLDLFree cholesterol to total lipids ratio in large VLDL
Triglycerides to total lipids ratio in large VLDL

Medium VLDL ratios
Phospholipids to total lipids ratio in medium VLDL
Cholesterol to total lipids ratio in medium VLDL
Cholesteryl esters to total lipids ratio in medium VLDLFree cholesterol to total lipids ratio in medium VLDL
Triglycerides to total lipids ratio in medium VLDL

Small VLDL ratios
Phospholipids to total lipids ratio in small VLDL
Cholesterol to total lipids ratio in small VLDL
Cholesteryl esters to total lipids ratio in small VLDLFree cholesterol to total lipids ratio in small VLDL
Triglycerides to total lipids ratio in small VLDL

Very small VLDL ratios
Phospholipids to total lipids ratio in very small VLDL
Cholesterol to total lipids ratio in very small VLDL
Cholesteryl esters to total lipids ratio in very small VLDLFree cholesterol to total lipids ratio in very small VLDL
Triglycerides to total lipids ratio in very small VLDLIDL ratios
Phospholipids to total lipids ratio in IDL
Cholesterol to total lipids ratio in IDL
Cholesteryl esters to total lipids ratio in IDLFree cholesterol to total lipids ratio in IDL
Triglycerides to total lipids ratio in IDL%

Phospholipids to total lipids ratio in large LDL
Cholesterol to total lipids ratio in large LDL
Cholesteryl esters to total lipids ratio in large LDL
Free cholesterol to total lipids ratio in large LDL
Triglycerides to total lipids ratio in large LDL%

Medium LDL ratios%

Phospholipids to total lipids ratio in medium LDL
Cholesterol to total lipids ratio in medium LDL

Chylomicrons and extremely large VLDL ratios

All listed biomarkers are available for Serum and Heparin plasma samples.

%

%
%
%
%
%

Large LDL ratios

Relative lipoprotein lipid concentrations
Phospholipids to total lipids ratio in chylomicrons
and extremely large VLDL
Cholesterol to total lipids ratio in chylomicrons
and extremely large VLDL
Cholesteryl esters to total lipids ratio in
chylomicrons and extremely large VLDLFree cholesterol to total lipids ratio in
chylomicrons and extremely large VLDL

Triglycerides to total lipids ratio in chylomicrons
and extremely large VLDL%

Biomarkers marked with * are not available for EDTA plasma samples.
Biomarkers marked with ** are not available for Citrate plasma samples.

%
%
%
%
%

%
%

Phospholipids to total lipids ratio in small LDL
Cholesterol to total lipids ratio in small LDL
Cholesteryl esters to total lipids ratio in small LDL
Free cholesterol to total lipids ratio in small LDL
Triglycerides to total lipids ratio in small LDL
Phospholipids to total lipids ratio in very large HDL
Cholesterol to total lipids ratio in very large HDL
Cholesteryl esters to total lipids ratio in very large HDL
Free cholesterol to total lipids ratio in very large HDL
Triglycerides to total lipids ratio in very large HDL
Phospholipids to total lipids ratio in large HDL
Cholesterol to total lipids ratio in large HDL
Cholesteryl esters to total lipids ratio in large HDL
Free cholesterol to total lipids ratio in large HDL
Triglycerides to total lipids ratio in large HDL

Phospholipids to total lipids ratio in medium HDL
Cholesterol to total lipids ratio in medium HDL
Cholesteryl esters to total lipids ratio in medium HDL
Free cholesterol to total lipids ratio in medium HDL
Triglycerides to total lipids ratio in medium HDL
Phospholipids to total lipids ratio in small HDL
Cholesterol to total lipids ratio in small HDL
Cholesteryl esters to total lipids ratio in small HDL
Free cholesterol to total lipids ratio in small HDL
Triglycerides to total lipids ratio in small HDL

%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%
%

%
%
%
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%
%

ABOUT USNightingale Health Plc provides a NMR(Nuclear Magnetic Resonance) based
metabolomics technology, supplying
biomarker analysis services for humanblood, urine, CSF and umbilical cord blood
samples. By measuring biomarkers frommultiple pathways in a single experiment,
Nightingale equips public health researcherswith comprehensive insights into the effects
of lifestyle factors and future diseaserisk, accelerating future breakthroughs inprecision medicine. In the long term, thecompany plans to fully integrate its services
into clinical practice, helping to empower
patients to follow-up on their own well-being
and take proactive steps to stay healthy.