LC column catalog
Reversed phase column

LC column catalog

L-column series

L-column, High performance column for LC, Since 1990.
L-column2, Super performance column for LC, Since 2007.

L-column
L-column2

L-column2

L-column- L-column2 ODS- L-column ODS- L-column2 C8- L-column ODS-P- L-column2 C6-Phenyl- L-column C8Line upL-column2

L-column, L-column2

Column developed in pursuit of convenience and high performance.

L-column and L-column2 is the column for reversed-phase high-performance liquid chromatography (RP-HPLC) thatenabled separation only by hydrophobic interaction because of inhibition of the secondary interaction with residual silanolgroups and metal impurities.

Characteristics of L-column
In 1990, the new end-capping method using high-temperature silylation was invented. The performance of L-column
ODS developed using this method was far superior to that of existing C18 columns, and it became a pioneer of the
new-generation end-capping method.

Characteristics of L-column2
L-column2, surpassing L-column in performance, was developed by upgrading the end-capping method. There is now ademand for RP-HPLC columns capable of both handling various issues of trace analysis due to the popularization of
LC/MS (/MS) and providing advanced analytical precision. L-column2 is an ideal column for meeting this demand.
- Sharper peaks for acidic, basic and coordination compounds due to extremely low silanol adsorption.
- High durability in a wide range of pH and temperature.
- Superior peak shapes in both acetonitrile/water and methanol/water eluents makes L-column2 convenient to use.
- Uniform lot to lot reproducibility of analyses due to extensive quality control measures.

Line up

L-column, L-column2
USP
category

Packing materials

L-column

L-column2

Particle
Size
(μm)

Pore
Size
(nm)

Micro column Semi-micro andgeneral-purpose
columnSemi
preparation
columnMetal-freecolumnInner diameter
less than 1 mm

Inner diameter 1 mm ormore and less than 10
mm

Inner diameter
10 mm or more

L-column ODS

L1

3, 5

12

○

○

○

-

L-column ODS-P

L1

5

30

○

○

-

-

L-column C8

L7

5

12

○

○

-

-

L-column2 ODS

L12, 3, 5

12

○

○

○

○

L-column2 C8

L7

3, 5

12

○

○

-

○

L-column2 C6-Phenyl

L 11

3, 5

12

○

○

-

○

Classification by inner diameter cited from JIS K 0124:2011

L-column ODS, L-column2 ODS, L-column ODS-P
USP category:L1
Usable pH range : pH 2 - pH 9Si

(L-column2 ODS: pH 1 - pH 9)

L-column C8, L-column2 C8
USP category:L7
Usable pH range : pH 2 - pH 7.5

Octadecyl (C18)Si
Octyl (C8)L-column2 C6-Phenyl
USP category: L 11
Usable pH range : pH 2 - pH 7.5

Si
Phenyl-Hexyl

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L-column2

Characteristic test
The characteristic test evaluates the following values:<Hydrogen bond property> k (Caffeine) / k (Phenol)
This is the value representing the influence of the hydrogen bond of the silanol group of packing materials and thesamples as represented by the ratio of the retention factors of caffeine with high hydrogen bond property and phenol withlow hydrogen bond property. The larger the value of "hydrogen bond property", the hydrogen bonds are easy to occur
between analyte and packing materials.
<Hydrophobicity> k (Amylbenzene) / k (Butylbenzene)
This is the value representing the magnitude of the retention force of packing materials as represented by the ratio of theretention factors of amyl benzene and butyl benzene. The larger the value of hydrophobicity, the higher thehydrophobicity of the packing materials and the longer the retention time.
<Stereoselectivity> k (Triphenylene) / k (o-Terphenyl)
This is the value representing the plane recognition ability as represented by the ratio of the retention factors of
triphenylene with a plane structure and o-terphenyl with a three-dimensional structure. The larger the value ofstereoselectivity, the stronger the retention of a compound with a plane structure.
L-column2 C8

L-column2 ODS
Cat.No.721070

L-column2 C6-Phenyl

Cat.No.721071

Hydrogen bond property : 0.46
Hydrophobicity: 1.46
Stereoselectivity: 1.61

Cat.No.721076

Hydrogen bond property : 0.40
Hydrophobicity: 1.35
Stereoselectivity: 1.38

3

3

1

7

1

2

Hydrogen bond property : 1.37
Hydrophobicity: 1.32
Stereoselectivity: 1.52
1

3

2

7

2
6

7
5
6
4

0

4

5 6

4

5

4

8
12
Time (min)

16

20

0

4

8
12
Time (min)

16

20

0

4

8
12
Time (min)16

20[Analytical conditions]
Column: 3 μm; Size: 4.6 mm I.D., 150 mm L.; Eluent: CH3OH/H2O (80/20); Flow rate: 1 mL/min; Temp.: 42 ºC; Detection: UV 254 nm; Inj. vol.: 1 μL
Sample: 1. Uracil (t0 marker)

2. Caffeine

3. Phenol

O
H3C
O

N

N
N

4. Butylbenzene

6. Amylbenzene

5. o-Terphenyl

7. Triphenylene

OH

CH3

N

CH3

The retention time of the analytes changes greatly due to each column (C18, C8 and phenyl columns) characteristics.
It is possible to improve the separation of analytes and shorten the analytical time by using each column properly.
L-column2 C8

L-column2 ODS
Cat.No.711020

04L-column2 C6-Phenyl

Cat.No.712021

8

12
16
Time (min)

20

24

28 0

4

Cat.No.712026

8

12
16
Time (min)

20

24

28 0

4

8

12
16
Time (min)

20

24

28

■ Agricultural chemicals (1 mg/L each)[Analyticai conditions]
Column: 3 μm or 5 μm; Size: 2.1 mm I.D.×150 mm L.
Eluent: A: CH3CN, B: 0.1% HCOOH in H2O; A/B, 5/95-100/0(0-40 min); Flow rate: 0.2 mL/min; Temp.: 40 ºC; Detection: ESI-MS(+); Inj. Vol.: 10 μL

2
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L-column2

Base silica
High purity silica, in which metal impurities are
reduced to the absolute limit, is used as the starting
material, facilitating analysis of coordination compounds.
White: silica skeleton
Black: silica porous body
includes: a silica skeleton

Silica test specifications (extract)
Analytical results for packing
materials

Specification

Surface area (ave.)

340 m2/g

Median pore diameter (ave.)

12 nm

Metal contents:

Al< 5.0 ppm

Fe

< 10.0 ppm

Na< 10.0 ppm

Ti< 0.5 ppm

Silica(Expansion)

End-capping
Various end-capping methods are used by column manufacturers, but the residual silanol groups cannot thoroughly beeliminated by the usual end-capping methods. The residual silanol groups of L-column and L-column2 are almost entirely
eliminated. Therefore, peak tailing derived from the residual silanol groups does not occur using L-column ODS and its
high durability, even in acidic or basic eluent, minimizes deterioration.
End-capping

Not end-capped C18
Trifunctional
C18

Kubelka-Munk units

residualsilanols

54
Kubelka-Munk units

Monofunctional
C18

3
2

residualsilanols

1

3
2
1
0

0
4000

4

4000

3000
Wavenumber (cm-1)

3000
Wavenumber (cm-1)

Liquid phase end-capping(Conventional column)
L-column2

L-column

Columns of other company
Brand C-3

C-3

Kubelka-Munk units

Brand D-1

L-column
L-column2
3700

4000
3000
Wavenumber (cm-1)

D-1

4000
3000
Wavenumber (cm-1)

4000
3000
Wavenumber (cm-1)

4000
3000
Wavenumber (cm-1)- C18, particle size: 5 μm

Wavenumber (cm-1)

3200-OH of silanols(3200 - 3700 cm-1)

The level of residual silanol groups is measured by FT-IR spectrum. The spectra of C18 without end-capping and the fully
end-capped L-column2 are shown. The spectrum region for C-H and O-H provides quantitative information as well as qualitative
identification. FT-IR spectra show virtually no presence of silanol groups on L-column2. In addition, the spectrum region for O-H (theright spectra) shows that L-column2 has the least residual silanol groups of any column tested.

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L-column2

Comparison between L-column2 and other columns
L-column2
1

Columns of other company

L-column

Cat.No.722070

Cat.No.622070

2

1

3

Brand C-3

Brand D-1

Brand E-1

2

1
3

2

2

3

2

1

1

1

0

8
16
Time (min)240Brand F-2

2

8
16Time (min)

24

0

8
16
Time (min)

24

0

3

8
16
Time (min)24

1. Propranolol■ Basic compounds

N
H

OH

8
16
Time (min)2402. Benazepril

CH3

O[Analytical conditions]
Column: C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (30/70)
Flow rate: 1 mL/min; Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL

0

3

24

3. Maprotiline
CH3

O

CH3

8
16
Time (min)

H
N

O

CH3

N
H

N

O
OH

O

L-column2
1

Columns of other company

L-column

Cat.No.722070

Cat.No.622070

Brand C-3

1

2

1
2

Brand D-1

2

Brand E-1

Brand F-2
1

1

2

2

2

1

0

1

2
3
Time (min)

4

0

1

2
3
Time (min)

4

0

1

2
3
Time (min)

4

0

1

2
3
Time (min)

4

0

1

2
3
Time (min)

4

0

1

2
3
Time (min)4■ Acidic compounds
1. Fomic acid

[Analytical conditions]
Column: C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/20 mM H3PO4 in H2O (2/98)
Flow rate: 1 mL/min; Temp.: 40 ºC; Detection: 210 nm; Inj. vol.: 1 μL

L-column2

O

H

H 3C

OH

OH

Columns of other company

L-column

Cat.No.722070

2. Acetic acidO

Cat.No.622070

Brand C-3

Brand D-1

Brand E-1

Brand F-2

N.D.

0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)10■ Coordination compound[Analytical conditions]
Column: C18, 5 μm; Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/20 mM H3PO4 in H2O (40/60)
Flow rate: 1 mL/min; Temp.: 40 ºC; Detection: 240 nm; Inj. vol.: 10 μL

Hinokitiol
O
HO
CH3
CH3

4
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L-column2

Improved durability
A durability test was carried out under high temperature conditions which accelerate deterioration of columns.
L-column2 ODS was stable for the longest time. Although it is silica-based, it shows superior durability even underalkaline conditions due to the extremely dense end-capping.
[Accelerated acidic eluent lifetime test]
Under acidic conditions, below pH 1, both theend-capping group and the ODS group are hydrolyzed.
Retention time decreases with the decrease of ODS
groups. Resolution decreases with the progression of
the hydrolysis. L-column2 ODS resists hydrolysis evenunder these harsh conditions to maintain retention andresolution for an extended lifetime.

Stability of N (Naphthalene) (%)

L-column

80

Brand D-1
Brand F-2
Brand H-1

60
40
20
0

■ Durability test (under pH 1, 90 ºC)[Durability test conditions]
Column: C18, 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3OH/2% TFA in H2O (10/90)
Flow rate: 1 mL/min; Temp.: 90 ºC

L-column2

100

0

50

100

150
200
Time (Hour)

250

300

L-column2 ODS
270 h

105 h
0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)10

Brand H-1
105 h
0

[Accelerated alkaline eluent lifetime test]
Dissolution of the base silica is accelerated in alkaline
eluent. Efficiency (theoretical plate number) drops
suddenly in these conditions. L-column2 ODS has
superior durability under high pH conditions due to theprotection of the silica surface afforded by the advanced
end-capping process.

5Time (min)L-column2

Stability of N (Naphthalene) (%)

100
80
60
40

L-column

Brand D-1
Brand F-2
Brand H-1

200

■ Durability test (pH 10, 50 ºC)[Durability test conditions]
Column: C18, 5 μm
Size: 4.6 mm I.D.×150 mm L.
Eluent: CH3OH/50 mM TEA in H2O (10/90)
Flow rate: 1 mL/min; Temp.: 50 ºC

10

0

20

40

60
Time (Hour)

80

100

L-column2 ODS
50 h

25 h
0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)

10

0

5
Time (min)10

Brand H-1
25 h05
www.cerij.or.jp

5Time (min)

10

50 h
0

5
Time (min)10L-column2

Superior reproducibility
Variation between product lots due to residual silanol groups is prevented by superior end-capping. Although
reproducibility of retention times of basic compounds is poor between product lots, the coefficient of variation of theretention times between the product lots is under 1% for L-column2 ODS. Regardless of the product lot, L-column2 ODS
provides the same results in HPLC analysis because of thorough quality control.
3■ Antidepressants[Analytical conditions]
Column: L-column2 ODS, 5 μm
Size: 4.6 mm I.D., 150 mm L. (Cat.No.722070)
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (60/40)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL

1. Doxepin

2. Imipramine

5
6
4

2
1

3. Amitriptyline

O
N

H3C

H3C

N

H3C

N
CH3

CH3

4. Mianserin

N
N
CH3

H3C

N

4

8
Time (min)CH3

5. Clomipramine

N

0

N

6. Trimipramine

Cl

tR(1)

tR(2)

tR(3)

tR(4)

tR(5)

tR(6)

A

5.115

5.796

7.008

7.647

9.546

12.508

B

5.180

5.868

7.103

7.755

9.669

12.648

C

5.184

5.823

7.022

7.687

9.609

12.608

CV(%)

0.647

0.626

0.725

0.707

0.640

0.575

N

CH3

N
CH3

16

Lot.No.

H3C
H3C

12

[Calibration curve]■ Hinokitiol

200000[Analytical conditions]
Column: L-column2 ODS, 5 μm
Size: 4.6 mm I.D., 150 mm L. (Cat.No.722070)
Eluent: CH3CN/20 mM H3PO4 in H2O (40/60)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 240 nm; Inj. vol.: 1 μL

Peak Area

15000010000050000
0Cartification (Certificate of analysis)

0

0.5

1.0
mg/L

1.5

2.0

L-column2

Specifications and test results of each product lot as well as test results for eachcolumn are supplied with the column.

Cer t i f i c at e o f an al y s i s
L-column2 ODS
Lot No. E0000

Analytical Results for Packing

Materials

Specification[ μm][m2/g][nm][mL/g]

0.0-0.0000-000
00.0-00.0
0.00-0.00[ pp m][ pp m][ p pm][ pp m]< 0.0< 00.0< 00.0< 0.0

A nal ysi s of unbonded Si l i ca gel
Median Particle Size(d50)*
Surface Area**
Median Pore Diameter**Pore Volume**
Metal contents***
Al
Fe
Na
Ti

Result* Coulter counter method, ** BET method, *** ICP-AES

Analytical Results for
Car bon cont ent

L-column2[% ]Chr omat ogr aphi c Resul t s
Test A k(Napht hal ene)
Test B k(Pyridine)/k(Phenol)
Test C k(Caffeine)/k(Phenol)
Test C k(Amylbenzene)/k(Butylbenzene)
Test C k(Triphenylene)/k(o-Terphenyl)
Test D k(Acenaphthene)/k(Amitriptyline)
Test E k(Oxine-copper)/k(Caffeine)
Test F
N(Formic acid)

Approved:

0.0-00.0

0.000-0.000
0.000-0.000
0.000-0.000
0.000-0.000
0.000-0.000
0.000-0.000
0.000-0.000
>0000

Date:

-

-

6
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L-column2 ODS

L-column2 ODS

Next generation high performance silica-based ODS column.

L-column2 ODS exceeds even the high performance of L-column ODS by virtue of its advanced new end-capping
method. It can be used for the analysis of acidic, basic and coordination compounds.

Properties
Particle size

2 μm, 3 μm, 5 μm

Pore size

12 nm

Surface area

340 m2/gSi
Octadecyl (C18)

Carbon contents 17%
Bonded phase

Octadecyl silyl (C18, ODS)

End-capping

Advanced end-capping

USP category

L1

Usable pH range pH 1 - pH 9

Comparison between L-column2 ODS and other columns
Columns of other company

L-column2 ODS
2
1

6

3
4 5

0

6

2 3
7

10

20

8

1

9

Time (min)

30

40

7 8

45

50

Brand D-1

L-column ODS

1

2

3

1
2

3

7

6
4

4

6

5

10

7

8

9

8
9

5

Brand E-1

10

Brand C-3

920

Time (min)

30

40

7

50

3 4

2

5

8

9

6

USP Tailing factor

4.0

3.0

Brand F-2

■ 2. Chlorpheniramine■ 3. Triprolidine■ 5. Diphenylpyraline■ 6. Homochlorcyclizine■ 9. Promethazine

1

2

3
4

6

7

5

89

2.0
0
1.0

L-column2
ODS

C-3

D-1

E-1

20

Time (min)3040■ Antihistamines← N.D.

010

F-2[Analytical conditions]
Column: C18, 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (40/60)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL
Sample: 1. Fexofenadine; 2. Chlorpheniramine; 3. Triprolidine;
4. Diphenhydramine; 5. Diphenylpyraline; 6. Homochlorcyclizine;
7. Hydroxyzine; 8. Astemizole; 9. Promethazine

7
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50

L-column2 ODS

L-column2 ODS is perfectly end-capped, so it does not adsorb analytes using any composition of eluents and provides asuperior peak shape. In almost all C18 columns, peak tailing of basic compounds using these eluents occurs, but in
L-column2 ODS does not occur.
Therefore, it can be used in a wide range of compositions of eluents. This is an important point when selecting a column.

Columns of other company

L-column2 ODS
Methanol

Acetonitrile

1

Methanol
1

2

2
2

2
2

1

1

Acetonitrile

2
1

1

1

Brand C-3

2
1

2
0

10
Time (min)

20

0

10
Time (min)20

1

Brand D-1

L-column ODS

2 2

1
1

2 1

2

1

2
2

1

1

1

Brand E-1

2
0

2

1

10
Time (min)

20

0

2

10
Time (min)

1

20

2

1

2

1
2

USP Tailing factor (Amitriptyline)

4.0

1

Brand F-2
2

3.0

2

2

1

1

1

2.0

2
0

10
Time (min)

1.0

20

0

10
Time (min)20■ Antidepressant0

L-column2
ODS

C-3

D-1

Eluent:■ CH3OH/25 mM Phosphate buffer pH 7(80/20)■ CH3OH/10 mM Ammonium acetate (65/35)■ CH3CN/25 mM Phosphate buffer pH 7 (55/45)■ CH3CN/10 mM Ammonium acetate (40/60)

E-1

F-2

[Analytical conditions]
Column: C18, 5 μm
Size: 4.6 mm I.D., 150 mm L.
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 5 μL
1. n-Buthyl p-hydroxybenzoate(I.S.)
O
O
HO

CH3

2. Amitriptyline
H 3C

N
CH3

Basic compounds show peak tailing due to their adsorption by residual silanol groups. Therefore, adsorption is inhibited using an
acidic eluent or a eluent including methanol to control peak tailing. Because the residual silanol groups show intrinsic activity using aneutral eluent or a eluent including acetonitrile as an organic solvent, peak tailing of basic compounds due to adsorption occurs whenusing poorly end-capped columns.

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L-column2 C8

L-column2 C8

Next generation high performance silica-based octyl column.

L-column2 C8 is end-capped using the same advanced method used for L-column2 ODS. This column is easy to use
because it provides sharp peaks in addition to it having high durability. L-column2 C8 is most suitable for reducing theanalysis time of hydrophobic compounds and conserving solvent.

Properties
Particle size

3 μm, 5 μm

Pore size

12 nm

Surface area

340 m2/gSi
Octyl (C8)

Carbon contents 10%
Bonded phase

Octyl silyl (C8)End-capping

Advanced end-capping

USP category

L7

Usable pH range pH 2 - pH 7.5

Comparison between C8 column and C18 column
log k
10.0

■ Reduction of analysis time
In general, the retention time for C8 columns is shortbecause they have lower retention ability than C18
columns. The analysis time of hydrophobic analytes canbe shortened, thus reducing the amount of solvent used.

Ethylbenzene
log Pow=3.2*

C18 column
C8 column

CH3

Propylbenzene
log Pow=3.69*

0.1

ze
en
lb

be

ty

yl

Bu

op

3

CH3

n-Butylbenzene
log Pow=4.38*

n-

Et

Pr

hy

lb

en

ze

Chromatogram of fat-soluble vitamins shows resultsfrom analysis of Fat-soluble vitamins using L-column2
ODS and L-column2 C8 under the same HPLC
conditions. Although the analysis of vitamin K using
L-column2 ODS columns takes longer because thelipophilicity of vitamin K is high, the analysis time using
L-column2 C8 is one quarter of that using L-column2
ODS.

nz

ne

en

e

ne

1.0

log Pow

4

5

CH3* reference:
http://anzeninfo.mhlw.go.jp/■ Alkylbenzenes[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3OH/H2O (85/15)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 254 nm; Inj. vol.: 1 μL

■ Fat-soluble vitamins
L-column2 ODS[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/CH3OH/H2O (76/19/5)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 285 nm; Inj. vol.: 2 μL

1. Vitamin D3

H 3C
CH3

CH3

CH3
HO

CH3

CH3

CH3

2
3

1

CH3

2

CH3

CH2

1

4

CH3

O

H 3C

Cat.No.722071

Analysis timeand used amount ofsolvents↓
1/4

2. Vitamin E

CH3

L-column2 C8

Cat.No.722070

3
4

HO

3. Vitamin E acetate

4. Vitamin K1

CH3
H 3C

CH3

O
H 3C

O
CH3

0

O

O

CH3

CH3

5

10

CH3

CH3

CH3

CH3

CH3

CH3
O

CH3

CH3

9
www.cerij.or.jp

1520Time (min)

25

30

35

0

5
10
Time (min)L-column2 C8■ Difference of separation behavior with C18 column
Compared to C18 columns, almost all analytes are
eluted faster on C8 columns, but the difference between
the retention time for C8 columns and that for C18
columns depends on the analytes. Because the elution
behavior of C8 columns is sometimes different from thatof C18 columns, C8 columns can sometimes improveseparation even if separation using C18 columns is notpossible.

L-column2 ODS

L-column2 C8

Cat.No.722070
1

Cat.No.722071

Improved separation

4

Rs(2,3)=1.53
2

3

Rs(2,3)
=3.75
2 3

6

5

4

1

6

5

■ Antdepressants[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (30/70)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL
Sample: 1. Sulpiride; 2. Desipramine; 3. Paroxetine; 4. Maprotiline;
5. Amoxapine; 6. Trazodone

0

10

20
Time (min)

30

40

0

10
20
Time (min)30Comparison between L-column2 C8 and other columns
Columns of other company

L-column2 C8
5
1

2

3

4

6
1

0

8

16
Time (min)

24

5

4

7

32

Brand F-4

61

■ Antihistamines[Analytical conditions]
Column: C8, 5 μm
Size: 4.6 mm I.D.×150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7.0 (40/60)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL
Sample: 1. Chlorpheniramine; 2. Triprolidine; 3. Diphenhydramine;
4. Diphenylpyraline; 5. Homochlorcyclizine; 6. Hydroxyzine;
7. Clemastine

Brand D-3

6

2+3

7

0

2

3

4

8

5

7

16
Time (min)24

32■ Durability test (under pH 1)
100
Stability of N (Naphthalene) (%)[Durability test conditions]
Column: C8, 5 μm
Size: 2.1 mm I.D.×150 mm L.
Eluent: CH3OH/H2O/TFA (10/90/1)
Flow rate: 0.2 mL/min; Temp.: 40 ºC

L-column2 C880
60

Brand D-3

40
20
0

0

50

100

150
200
Time (Hour)

250

300

10
www.cerij.or.jp

L-column2 C6-Phenyl

L-column2 C6-Phenyl

Next generation high performance silica-based phenyl column.

The main retention mechanisms of L-column2 C6-Phenyl are π-π interaction and hydrophobicity.
L-column2 C6-Phenyl can use the same eluent as an C18, and can obtain some different separation patterns.

Properties
Particle size

3 μm, 5 μm

Pore size

12 nm

Surface area

340 m2/g

Si
Phenyl-Hexyl

Carbon contents 13%
Bonded phase

Phenyl-hexyl silyl (C6-Phenyl)End-capping

Advanced end-capping

USP category

L 11

Usable pH range pH 2 - pH 7.5

Comparison between phenyl column and C18 column■ π-π interaction
The separation behaviors of nitrobenzenes in different
stationary phases were compared.
The retention of C18 column and Phenyl column without
alkyl spacer becomes stronger in order of stronghydrophobicity of nitrobenzenes.
The retention of phenyl column with spacer becomesstronger as the number of nitro groups increases, andthe retention mechanism by π-π interaction appears
strongly.

Phenyl column
Spacer: 0 3
2 1

L-column2 ODS■ Nitrobenzenes

O2N

Cat.No.722076

0

4

3

Spacer: 6
2

NO2

NO2

O2N

L-column2 C6-Phenyl

3

2. 1,3-Dinitrobenzene 3. 1,3,5-Trinitrobenzene
log Pow=1.49*
log Pow=1.18*

NO2

12

Cat.No.722070[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D.×150 mm L.
Eluent: CH3OH/H2O (50/50)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 210 nm; Inj. vol.: 1 μL
1. Nitrobenzene
log Pow=1.86*

3Spacer: 2

1

2

1

Time (min)

8

12

0

4

Time (min)812* References:
http://anzeninfo.mhlw.go.jp/
NO2■ Basic drugs

L-column2 ODS[Analytical conditions]
Column: 5 μm
Size: 2.1 mm I.D., 150 mm L.
Eluent: A: 0.1% HCOOH in CH3CN; B: 0.1% HCOOH in H2O
A/B, 5/95-70/30 (0-45 min)Flow rate: 0.3 mL/min
Temp.: 40 ºC; Detection: ESI-MS(+); Inj. vol.: 1 μL
Sample: 1. Yohimbine; 2. Prazoin; 3. Trazodone; 4. L-Alprenolol;
5. Isopropylantipyrine; 6. Clemastine; 7. Ibudilast

L-column2 C6-Phenyl

Cat.No.712020
25

Different
separation
patterns

1
2 3

11
www.cerij.or.jp

2030Time (min)

3
7

6

10

5

4

7

4

L-column2 C6-Phenyl with hexyl spacer is designed to takefull advantage of the π-π interaction.
The unique structure is the feature of L-column2 C6-Phenyl
and allows column selection for improved separation.

Cat.No.712026

1

6

40 10

20
30
Time (min)40L-column2 C6-Phenyl■ Coordination compound
L-column2 ODS[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/20 mM H3PO4 (10/90)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 294 nm; Inj. vol.: 2 μL(2 g/L)

L-column2 C6-Phenyl

Cat.No.722070

3

Cat.No.722076

3

3. Levofloxacin
H 3C

H
O

N

N

N

HO

F
O

Rs(2,3)=0.51

CH3

1. Impurity A
2. Impurity B

Improved
separation

2O

Rs(2,3)=2.33

1

Chromatogram is comparing impurities in levofloxacin under
the same analytical conditions. Levofloxacin is a newquinolone synthetic antibacterial agent that is easy tocoordinate with metals.
L-column2 C6-Phenyl shows some good peak shapes, andmore impurities than C18 have been separated.

8

2

1

12
16
Time (min)

20

8

12
16
Time (min)20■ Calcium antagonists
L-column2 ODS[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (50/50)
CH3OH/25 mM Phosphate buffer pH 7 (70/30)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 235 nm; Inj. vol.: 1 μL
Sample: 1. Nifedipine; 2. Nitrendipine; 3. Diltiazem; 4. Verapamil;
5. Nicardipine

L-column2 C6-Phenyl

Acetonitrile
1

1
3

2

Retention:
Small
4

L-column2 C6-Phenyl has greater retention time than ODS
column, when methanol is used as the eluent.
It is considered that methanol does not interfere with the π-πinteraction between the phenyl group and the analytes
because methanol does not have π electrons in its structure.

3
2
4

5

5

Methanol
1

3

1 3

2
4

0

8

Retention:
Large

5

2

5

4

16
Time (min)

24

0

8

16
Time (min)24Comparison between L-column2 C6-Phenyl and other columns
Columns of other company

L-column2 C6-Phenyl

Brand A-3

4
1

1

2

Brand A-3
4

2

3

0

5

10
Time (min)

15

20

0

5

10
Time (min)

3

15

20
1

Brand C-7

4

2

■ Basic drugs[Analytical conditions]
Column: Phenyl-Hexyl, 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL
Sample: 1. Butyl p-Hydroxybenzoate; 2. Clemastine;
3. Propranolol; 4. Dextromethorphan

Brand C-70

510Time (min)

15

3

20

0

5

10
Time (min)

15

20

12
www.cerij.or.jp

L-column Micro

L-column Micro

High-performance column for nano and micro HPLC.

L-column Micro is a nano/micro column with 0.075–0.3 mm I.D. It is a high-performance column that was manufactured
by combining low-adsorption packing materials, including L-column2 ODS, an originally developed packing techniqueand a column structure with a small dead volume.

Column Structure[PEEK-sleeved type]This column is easy to handle because it is a fused silica
capillary with an outer sleeve of PEEK resin. Connection
to MS is easy because connecters are attached to thecolumn.

PEEK

Packing
material
Fused silica capillary

1/16”O.D.

(Expansion)
Packing
material

Fused silica capillary, PEEK
Connection: 1/16 inch stainless-steel connector

Fused silica capillary
Electron microgram (0.075 mm I.D.).

Cover ratio■ Tryptic digest of BSA[Analytical conditions]
Column: C18, 3μm
Size: 0.075 mm I.D., 150 mm L.
Eluent: CH3CN/0.1% HCOOH in H2O
Flow rate: 0.25 μL/min
Temp.: Ambient

L-column Micro (Cat.No.611380)
Brand I
60
Sequence Coverage (%)

BSA tryptic digest was analyzed using LC/MS/MS andthe sample concentration and cover ratio wasdetermined using L-column Micro and another brand. A
higher cover ratio means that more amino acidsequences are read.

55
47

40

36
25

20

12
2

0

L-column Micro shows advantage at all concentrationlevels, but excels at the lower levels. With superior
end-capping and very high theoretical plate numbers percolumn, L-column Micro permits identification of many
proteins and is the optimum choice for proteome
analysis.
■ Tryptic digest of BSA (50 fmol)[Analytical conditions]
Column: L-column2 ODS, 3 μm
Size: 0.075 mm I.D., 150 mm L.; PEEK-sleeved type (Cat.No.711400)
Eluent: CH3CN/0.1% HCOOH in H2O, gradient elution
Flow rate: 0.2 μL/min
Temp.: Ambient- Courtesy of Dr. Ueda, RIKEN center for Genomic Medicine

13
www.cerij.or.jp

20200(fmol)

Score: 2207
Sequence Coverage: 79%2000

L-column Micro

Low adsorption and high efficiency
L-column Micro can separate many peptides because of
the low-adsorption packing materials. This is effective forthe identification of proteins. The column has a hightheoretical plate number and high durability because it ispacked homogeneously by a patented technique.

Brand I

L-column Microseparate many
peptides■ Tryptic digest of Enolase 1 (100 fmol)[Analytical conditions]
Column: C18, 3 μm
Size: 0.075 mm I.D., 150 mm L.
Eluent: A: 0.1% HCOOH in CH3CN; B; 0.1% HCOOH in H2O
A/B, 5/95-40/60 (0-30 min)Flow rate: 0.25 - 0.3 μL/min
Temp.: Ambient; Inj.vol.: 1 μL

Trap column
In proteomic analysis using LC/MS/MS, the trap column
is indispensable for increasing the injection volume.
This trap column retains the target constituents firmly
and its media has little irreversible adsorption of thetarget constituents. Therefore, the actual loss of target
constituents is negligible. In the range of 20 - 1000 ng forthe insulin B chain, a linear relationship is obtained
between the injection volume and the peak area.

A linear relationshipis obtained between
the injection volume
and the peak area

(×105)

Peak Area

60■ Insulin, chain B (20 - 1000 ng)

40

20

0

0

200

[Analytical conditions]
Trap column: L-column ODS, 5 μm
Size: 0.3 mm I.D., 5 mm L. (Cat.No.652450)
Eluent:A: 0.1% HCOOH in CH3CN; B; 0.1% HCOOH in H2O
A/B, 5/95-40/60-90/10 (0-10-15 min)Detection: UV 215 nm; Inj.vol.: 1 μL

A small dead volume, low-adsorption property and highretention ability that traps target constituents are
required effectively in a trap column.
L-column trap column does not decrease the theoreticalplate number because of its very small dead volume. Not
only that, because it is a cartridge-type trap column, it ismore economical.
■ Tryptic digest of BSA (500 fmol)

400
600
Injection mass (ng)

800

1000

L-column trap column

Size: 0.3 mm I.D., 5 mm L.
(Cat.No.652450)

Brand D

Size: 0.2 mm I.D., 150 mm L.

Low retention
ability[Analytical conditions]
Column: L-column ODS, 3 μm
Size: 0.075 mm I.D., 50 mm L.; Non-sleeved type (Cat.No.611380)
Eluent: A: 0.1% HCOOH in CH3CN; B; 0.1% HCOOH in H2O
A/B, 5/95-40/60 (0-60 min)Flow rate: 0.25 - 0.3 μL/min
Detection: UV 215 nm; Inj.vol.: 1 μL

10Holder20

30

Time (min)

40

50

60

Cartridge
Cartridge trap column (0.3 mm I.D., 5 mm L.)
Particle size: 5 μm

14
www.cerij.or.jp

L-column ODS

L-column ODS

Established, high-performance standard C18 column.

In 1990, L-column ODS was introduced as a column whose secondary interaction with the residual silanol groups waseliminated by a new end-capping method using high-temperature silylation. At the time, the performance of L-column
ODS was far superior to that of existing C18 columns, and it became a pioneer of the new-generation end-capping
method.

Properties
Particle size

3 μm, 5 μm

Pore size

12 nm

Surface area

340 m2/gSi
Octadecyl (C18)

Carbon contents 17%
Bonded phase

Octadecyl silyl (C18, ODS)

End-cappingHigh temperature gas phase end-capping

USP category

L1

Usable pH range pH 2 - pH 9

Comparison between L-column ODS and other columns
Columns of other company

L-column ODS
12

Brand A-1

2

4
6
Time (min)2

Brand B-1

4
6Time (min)■ Pyridine, Phenol Test

2

Brand C-1

4
6
Time (min)1. Pyridine

2

46Time (min)2. Phenol

Brand A-1

8

4
6
Time (min)2

Brand F-1

4
6Time (min)2

Brand G-1

4
6Time (min)2

46Time (min)Columns of other company

L-column ODS

46Time (min)2

Brand E-1

Column performance can be evaluated using pyridine which is abasic compound, and phenol which is an acidic compound.
L-column obtained a symmetric and sharp peak.

OH
N

2

Brand D-1

2

4
6
Time (min)■ Coordination compound

Brand B-1

8

2

N

4
6
Time (min)Brand C-1

8

2

4
6
Time (min)8

Brand D-1

2

O
O

Cu

N

Oxine-copper[Analytical conditions]
Column: C18, 3 μm
Size: 2 mm I.D. or 2.1 mm I.D., 150 mm L.

15
www.cerij.or.jp

46Time (min)8

Brand E-1

2

4
6Time (min)Brand F-1

8

2

4
6
Time (min)Brand G-1

8

2

4
6
Time (min)8L-column ODS-P

L-column ODS-P Wide pore C18 column for analysis of protein and peptide.
L-column ODS-P is ideal for the analysis of proteins and peptides. The base silica has a pore diameter of 30 nm.
Adsorption is minimized and proteins and peptides elute with sharp peaks. Biological samples are often analyzed using
TFA in the eluent and L-column ODS-P is exceptionally stable in strongly acidic eluent.

Properties
Particle size

5 μm

Pore size

30 nm

Surface area

150 m2/gSi
Octadecyl (C18)

Carbon contents 9%

White: silica skeleton
Black: silica porous body
includes: a silica skeleton

Bonded phase

Octadecyl silyl (C18, ODS)

End-cappingHigh temperature gas phase end-capping

USP category

L1

12 nm

Usable pH range pH 2 - pH 9

30 nm

Silica(Expansion)
Pore size (image)
Left: L-column ODS, rigth: L-column ODS-P

Role of Pore diameter in protein and peptide analysis
A molecular weight of under 10000 does not show different peak shape between 12 nm pore diameter and 30 nm pore
diameter. On the other hand, a molecular weight of over 10000 shows a broad peak when analyzed on the 12 nm
L-column ODS and the main component is not separated from the impurities. Using 30 nm L-column ODS-P, the main
component is separated from the impurities with good peak shape.
Analytes of molecular weight of approximately 10,000 to 20,000 are suitable for L-column ODS-P.

Pore size: 12 nm
L-column2 ODS
L-column ODS(M.W.)

Pore size: 30 nm
L-column ODS-P

5000

Insulin, chain B(MW: 3500)

10000

20000

15000

Cytochrome C(MW: 12400)

25000

Myoglobin(M.W.: 17800)

30000

40000

Pore size 30 nm:
L-column ODS-P

50000

Ovalbumin(M.W.: 44000)

60000

BSA
(M.W.: 66000)(Cat.No.622077)

Pore size 12 nm:
L-column ODS(Cat.No.622070)
8

10Time (min)

12

8

10
Time (min)

12

14

16
Time (min)

18

18

20
Time (min)

22

12

14
Time (min)16

The retention of high molecular weight proteins suchas ovalbumin and BSA is less because they arehydrophobic interactions only on the stationary phase(ODS) surface.

■ Peptides, Proteins[Analytical conditions]
Column: C18, 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: A: 0.1% TFA in CH3CN; B: 0.1% TFA in H2O
A/B, 25/75-60/40-60/40 (0-20-25 min)Flow rate: 1 mL/min
Temp.: 25 ºC; Detection: UV 220 nm; Inj. vol.: 10 μL

16www.cerij.or.jp

L-column C8

L-column C8high-performance standard octyl column.

L-column C8 is end-capped using the same advanced method used for L-column ODS. L-column C8 is most suitable for
reducing the analysis time of hydrophobic compounds and conserving solvent.

Properties
Particle size

5 μm

Pore size

12 nm

Surface area

340 m2/gSi
Octyl (C8)

Carbon contents 10%
Bonded phase

Octyl silyl (C8)End-cappingHigh temperature gas phase end-capping

USP category

L7

Usable pH range pH 2 - pH 7.5■ Antihistamines
L-column ODS[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/50 mM Phosphate buffer pH 6.8 (43/57)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 220 nm; Inj. vol.: 1 μL

2. Diphenhydramine

1

CH3
N

H

CH3

O

N

Shortened
analysis time

3. Clemastine
H 3C

N

N

Cl
H

O

CH3

I.S. 3

I.S.

CH3

3

CH3

Cl

Cat.No.622071

2

2
1

1. Chlorpheniramine

L-column C8

Cat.No.622070

I.S. n-Buthyl p-hydroxybenzoate
O

0
O

4

8
12
Time (min)

CH3

16

20

0

4

8
12
Time (min)

16

20

HO

■ Sulfa drugs
L-column ODS[Analytical conditions]
Column: 5 μm
Size: 4.6 mm I.D., 150 mm L.
Eluent: CH3OH/50 mM CH3COONH4 in H2O (15/85)
Flow rate: 1 mL/min
Temp.: 40 ºC; Detection: 270 nm; Inj. vol.: 15 μL
Sample: 1. Sulfadiazine; 2. Sulfisomidine; 3. Sulfathiazole;
4. Sulfamethoxazole; 5. Sulfamerazine;
6. Sulfamonomethoxine; 7. Sulfadoxine;
8. Sulfamethoxypyridazine; 9. Sulfadimidine;
10. Sulfadimethoxine; 11. Sulfaquinoxaline

L-column C8

Cat.No.622070

23 5
46

Change ofseparation behavior

7

Cat.No.622071

1

1

2
34
56
7

89

8 9
10
11

0

17
www.cerij.or.jp

1020Time (min)

30

10
11

0

10

20
Time (min)30Line upLine up L-column
LengthPacking materials

Particle size

Inner diameter

L-column ODS(USP category: L 1)

3 μm

1.5 mmCat.No.

2.1 mmCat.No.

6116303.0 mmCat.No.

621650

30 mm

35 mm

50 mm

75 mm

611130611240100 mm 150 mm 250 mm
611160611010611140611640611170611020611220621490621600621330621260621320621150621460621180621070621080612130612160612010612140612170612020612220622330622260622320622040622310622070622080Price (JPY)
4.6 mmCat.No.

621250Price (JPY)
20.0 mm

Cat.No.

641230Price (JPY)5 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mm612240Cat.No.
Price (JPY)

4.0 mmCat.No.

4.6 mmCat.No.

622250622150622180Price (JPY)
6.0 mmCat.No.

622090Price (JPY)
10.0 mm

Cat.No.

642100Price (JPY)
20.0 mm

Cat.No.

642230642520642120Price (JPY)
L-column ODS-P(USP category: L 1)5 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mm612247612137612167612017612147612177612027612227622267622327622087

Cat.No.
Price (JPY)

4.6 mmCat.No.

622257622157622187622077612131612161612011612141612171612021612221622261622321622071622081Price (JPY)
L-column C8(USP category: L 7)5 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

612241Price (JPY)
4.6 mmCat.No.

622251622151622181Price (JPY)- Connection type: 1/16” Waters.

18
www.cerij.or.jp

Line upLine up L-column2
Packing materials: L-column2 ODS (USP category: L 1)
LengthParticle size
2 μm

Inner diameter
1.5 mmCat.No.

2.1 mmCat.No.

10 mm

20 mm

30 mm

713780713770

35 mm

50 mm

75 mm

100 mm

150 mm

713630713140713640713160713010713170713020723650723490723600723330250 mmPrice (JPY)
3.0 mmCat.No.
Price (JPY)

3 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mm711130711780711770Cat.No.

711630711240721650711160711010711140711640711170711020711220721490721600721330721260721320721150721460721180721070721080712130712160712010712140712170712020712220722330722260722320722040722310722070722080Price (JPY)
4.6 mmCat.No.

721250Price (JPY)
20.0 mm

Cat.No.

741230Price (JPY)5 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

712240Price (JPY)
4.0 mm
4.6 mmCat.No.
Cat.No.

722250722150722180Price (JPY)
6.0 mmCat.No.

722090Price (JPY)
10.0 mm

Cat.No.

742510742100742520742120100 mm

150 mm

250 mm

711161711011Price (JPY)
20.0 mm

Cat.No.

742230Price (JPY)- Connection type: 1/16” Waters.

Packing materials: L-column2 C8 (USP category: L 7)
LengthParticle size
3 μm

Inner diameter

10 mm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

20 mm

30 mm

35 mm

50 mm

75 mm

711131
711781711771711631711241721651711141711641711171711021711221721491721601721331721261721321721151721461

721181721071721081712131712161712011712141712171712021712221722331722261722321722181722071722081Price (JPY)
4.6 mmCat.No.

721251Price (JPY)5 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

712241Price (JPY)
4.6 mmCat.No.

722251Price (JPY)19- Connection type: 1/16” Waters.

www.cerij.or.jp

722151

L-column2

Line up L-column2
Packing materials: L-column2 C6-Phenyl (USP category: L 11)
LengthParticle size

Inner diameter

3 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

10 mm

20 mm

30 mm

35 mm

711786711776711636711246

50 mm

75 mm

711136721656

100 mm

150 mm

250 mm

711166711016711146711646711176711026711226721496721606721336721266721326721156721466721186721076721086712136712166712016712146712176712026712226722266722326722076722086

Price (JPY)
4.6 mmCat.No.

721256

Price (JPY)5 μm

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

712246

Price (JPY)
4.6 mmCat.No.

722256722156722186

Price (JPY)- Connection type: 1/16” Waters.

Metal-free column
LengthPacking materials

Particle size

L-column2 ODS(USP category: L 1)

3 μm

Inner diameter
2.0 mmCat.No.

30 mm

50 mm

100 mm

150 mm

250 mm

731630731140731170731020731220732140732170732020732220731141731171731021731221732141732171732021732221731146731176731026731226732146732176732026732226

Price (JPY)5 μm

2.0 mmCat.No.
Price (JPY)

L-column2 C8(USP category: L 7)

3 μm

2.0 mm5 μm

2.0 mm3 μm

2.0 mm5 μm

2.0 mmCat.No.

731631Price (JPY)Cat.No.
Price (JPY)

L-column2 C6-Phenyl(USP category: L 11)

Cat.No.

731636

Price (JPY)Cat.No.
Price (JPY)- Connection type: 1/16” Waters.

L-column2 Metal-free column
Glass
Stainlesssteel tube

Glass lined
stainless-steel
tube

Packing materials (L-column2)
Polymer frit
L-column2 metal free columns are improved the peak shape,
carryover and S/N in the analysis of proteins, peptides andcoordination compounds.

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L-column Micro: Nano column / micro column with 0.075 mm I.D. - 0.3 mm I.D.
LengthParticle size: 2 μm

Particle size: 3 μm

Particle size: 5 μm

Packing materials

Inner diameter

50 mm

150 mm

500 mm

50 mm

150 mmL-column2 ODS(USP category: L 1)

0.075 mm

Cat.No.

7114107114207118007124107124200.1 mmCat.No.

7113907114007118107123907124000.2 mmCat.No.

7132907133007112907113007122907123000.3 mmCat.No.

71327071328071127071128071227071228050 mm

150 mmPrice (JPY)
L-column2 C8(USP category: L 7)

0.075 mm

Cat.No.

711411711421712411712421

0.1 mmCat.No.

711391

711401712391712401

0.2 mmCat.No.

711291711301712291712301

Cat.No.

711271711281712271

712281

0.3 mmPrice (JPY)
L-column2 C6-Phenyl(USP category: L 11)

0.075 mm

Cat.No.

711416711426712416712426

0.1 mmCat.No.

711396711406712396712406

0.2 mmCat.No.

711296711306712296712306

0.3 mmCat.No.

711276711286712276712286

Price (JPY)
L-column ODS(USP category: L 1)

0.075 mm

Cat.No.

6114106114206124106124200.1 mmCat.No.

6113906114006123906124000.2 mmCat.No.

6112906113006122906123000.3 mmCat.No.

611270611280612270612280Price (JPY)
L-column ODS-P(USP category: L 1)

0.075 mm

Cat.No.

612417612427

0.1 mmCat.No.

6123976124070.2 mmCat.No.

612297612307

Cat.No.

612277612287

0.3 mmPrice (JPY)- Connection type: 1/16” Valco type.

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Line upLine upGuard column, Trap column (cartridge type)
Packing materials

Particle size

L-column2 ODS

5 μm

Length0.3 mm
2.0 mm

5 μm

L-column2 C85 μm

L-column2 C6-Phenyl

5 μm

L-column ODS

5 μm

L-column ODS-P

5 μm

L-column C8

HolderInner diameter

Direct typeNon-direct typeSpecification

Cat.No.

5 mm

Cartridge (3 pcs)

7524505 mm

Cartridge (3 pcs)

7523304.6 mm

10 mm

Cartridge (3 pcs)

7520500.3 mm

5 mm

Cartridge (3 pcs)

7524532.0 mm

5 mm

Cartridge (3 pcs)

752333

4.6 mm

10 mm

Cartridge (3 pcs)

752053

0.3 mm

5 mm

Cartridge (3 pcs)

752456

2.0 mm

5 mm

Cartridge (3 pcs)

752336

4.6 mm

10 mm

Cartridge (3 pcs)

752056

0.3 mm

5 mm

Cartridge (3 pcs)

6524502.0 mm

5 mm

Cartridge (3 pcs)

6523304.6 mm

10 mm

Cartridge (3 pcs)

6520500.3 mm

5 mm

Cartridge (3 pcs)

6524572.0 mm

5 mm

Cartridge (3 pcs)

652337

4.6 mm

10 mm

Cartridge (3 pcs)

652057

0.3 mm

5 mm

Cartridge (3 pcs)

6524532.0 mm

5 mm

Cartridge (3 pcs)

652333

4.6 mm

10 mm

Cartridge (3 pcs)

652053

For 2.0 mm I.D.

(1 pc)

651332

For 4.6 mm I.D.

(1 pc)

651052

For 0.3 mm I.D.

(1 pc)

652452

For 2.0 mm I.D.

(1 pc)

652332

For 4.6 mm I.D.

(1 pc)

652052

HolderPrice (JPY)

Cartridge
Direct typeNon-direct typePre-column filter
Specification

Cat.No.

Filter (1 pc), holder W+W (1 pc)

Connection type: Column side, Waters type; LC tubing side, Waters type653002Filter (1 pc), holder W+U (1 pc)

Connection type: Column side, Waters type; LC tubing side, UPLC® type653004Filter (5 pcs)

For replacement

653003

HolderPrice (JPY)

FilterTube of LC system
stainless-steel nut
1/16”O.D.
Cat.No.653002
Waters type

3.5 mm1/16”O.D.

Analytical column(Waters type)
Cat.No.653004

UPLC® type

2.3 mm- Length sometime depends on the manufacture.

The pre-column filter prevents shavings generated from theplunger seal, insoluble matters in samples, etc., fromentering the column. The pre-column filter is indispensable
for maintaining column longevity. The theoretical plate
number does not decrease when attaching L-column
pre-column filter because its direct connection with theanalytical column prevents the generation of a dead volume.
L-column pre-column filter can be installed on most HPLC
systems because it has two types, Waters and UPLC®.

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LC column catalog
L-column, L-column2

Chemicals Evaluation andResearch Institute, Japan■ The product specifications are as of January 1, 2020. Please note that they may be changed without prior notice.
■ For questions about part prices or ordering, please contact the Chromatography Department of CERI or a nearby agency. Details areprovided on the page for the Chromatography Department of our website. Please use them as a reference.

https://www.cerij.or.jp
CERI Tokyo
Chromatography department

ISO
9001
Design,Development,Production
and supply of Column
for GC and HPLCe-mail chromato@ceri.jp

FAX +81-480-37-2521
1600 Shimotakano, Sugito-machi, Kitakatsushika-gun,
Saitama 345-0043, JapanⒸ2019 CERI, Japan 2019/10; 2020/01