Sepharose Fast Flow ion
exchange resins and prepacked
column formats
ION EXCHANGE CHROMATOGRAPHY

The popularity of Sepharose™ Fast Flow ion exchange (IEX)
chromatography resins reflects the important role they play inprotein purification today. The reliability and well-documented
performance of the resins have made them a common choicefor capture and intermediate purification of proteins in bothresearch and industry.
Sepharose Fast Flow ion exchangers offer many practicaladvantages:•

high binding capacity and good flow properties•

high chemical and physical stabilities•

reliable and reproducible performance•easy and effective cleaning-in-place (CIP)/sanitization•

various, convenient prepacked column formats•

predictable scale-up

IEX chromatography
IEX is probably the most frequently used and versatile method
for fractionating proteins and peptides, even those with small
differences in charge. Furthermore, binding and elution conditions
are easy to optimize, resulting in fast separations that are
reproducible and cost-effective to scale up.

Fig 1. Sepharose Fast Flow ion exchangers, available in different formats forprocess development to production scale, are a common choice in preparative
protein separations.

The technique is based on reversible interactions between
charged molecules and immobilized ion exchange groups of
opposite charge. The charged molecules are allowed to bind to
the separation resin at low ionic strength and are eluted with asalt or pH gradient. Continuous gradient elution is most often
used when good resolution is needed, while simple stepwise
gradient elution is employed for sample preparation, groupseparation, or concentration.

Sepharose Fast Flow ion exchangers include resins that are called
weak (CM, DEAE, and ANX) or strong (SP and Q). The binding
capacity of weak ion exchangers varies considerably morewith pH than that of strong ion exchangers, which might affectselectivity. In contrast, the ligands of strong ion exchangers
remain charged, with a consistently high capacity maintained
over a broad working pH range.

cytiva.com

Chromatography resin characteristics

give fast separation cycles, which is especially important early inpurifications when rapid enrichment is required. For washing andequilibration, flow velocities can be extended up to 750 cm/h.

Sepharose Fast Flow ion exchange resins comprise SP Sepharose
Fast Flow, CM Sepharose Fast Flow, Q Sepharose Fast Flow, DEAE
Sepharose Fast Flow, and ANX Sepharose 4 Fast Flow (high sub).

ANX Sepharose 4 Fast Flow (high sub) is based on a cross-linked,
spherical, 4% agarose matrix, resulting in a resin with higher
porosity, which is particularly useful for purifying high molecularweight proteins. Like the other Sepharose Fast Flow ion exchange
resins, ANX has high flow rates for processing.

SP, CM, Q, and DEAE are based on a robust, cross-linked,
spherical, 6% agarose matrix with very good flow properties andhigh loading capacity. Typical flow velocities of 300 to 400 cm/h
through a 15 cm high bed at a pressure of 1 bar (14.5 psi, 0.1 MPa),
Table 1.Characteristics of Sepharose Fast Flow ion exchangers

Cation exchangers
MatrixParticle size, d50V

SP Sepharose Fast Flow

CM Sepharose Fast Flow

Cross-linked agarose, 6%, spherical

Cross-linked agarose, 6%, spherical~ 90 µm~ 90 µm

Type of resin

Strong cation

Weak cation

Charged group-CH2-CH2-CH2SO3-Ionic capacity

0.18 to 0.25 mmol H /mL resin

Dynamic binding capacity~ 70 mg ribonuclease A/mL resin

Pressure/flow characteristics3

400 to 700 cm/h at < 0.1 MPa in a XK 50/30 column
with 5 cm diameter and 15 cm bed height (at 25°C
using buffers with the same viscosity as water)

300 to 600 cm/h at < 0.1 MPa in a XK 50/30 column
with 5 cm diameter and 15 cm bed height (at 25°C
using buffers with the same viscosity as water)

pH stability, operational4

4 to 13

4 to 13

pH stability, CIP

3 to 142 to 14

1-O-CH2COO 0.09 to 0.13 mmol H+/mL resin+

5~ 50 mg ribonuclease A/mL resin2

2

pH ligand fully charged6

Entire pH range

Above 6

Chemical stability

Stable to commonly used aqueous buffers, 1.0 M NaOH , 8 M urea, 6 M guanidine hydrochloride, and 70% ethanol

Avoid

Oxidizing agents, cationic detergents and buffers

Storage

20% ethanol in 0.2 M sodium acetate, 4˚C to 30˚C

7

20% ethanol, 4˚C to 30˚C

Anion exchangers
Q Sepharose Fast Flow

DEAE Sepharose Fast Flow

ANX Sepharose 4 Fast Flow (high sub)Matrixcross-linked agarose, 6%, sphericalcross-linked agarose, 6%, sphericalcross-linked agarose, 4%, spherical

Particle size, d50V1~ 90 µm~ 90 µm~ 90 µm

Type of resin

Strong anion

Weak anion

Charged group-N (CH3)3-N (C2H5)2H

Ionic capacity

0.18 to 0.24 mmol Cl /mL resin

0.11 to 0.16 mmol Cl /mL resin

0.13 to 0.18 mmol Cl-/mL resin~ 42 mg BSA/mL resin8~ 110 mg HSA/mL resin2~ 5 mg/thyroglobulin/mL resin9+-

Dynamic binding capacity
Pressure/flow characteristics+

3

Weak anion-N+(C2H5)2H10

10-400 to 700 cm/h at < 0.1 MPa in a
300 to 600 cm/h at < 0.1 MPa in a
≥ 200 cm/h at 0.1 MPa in a XK50/60
XK 50/30 column with 5 cm diameter XK 50/30 column with 5 cm diameter column with 5 cm diameter and 25 cm
and 15 cm bed height (at 25°C using and 15 cm bed height (at 25°C using bed height (at 20°C using buffers withbuffers with the same viscosity
buffers with the same viscosity
the same viscosity as water)as water)as water)

pH stability, operational4

2 to 122 to 123 to 13

pH stability, CIP5

2 to 142 to 142 to 14

pH ligand fully charged6

Entire pH range

Below 9Chemical stability

Stable to commonly used aqueous buffers, 1.0 M NaOH , 8 M urea, 6 M guanidine hydrochloride, and 70% ethanol

Avoid

Oxidizing agents, anionic detergents and buffers

Storage

20% ethanol, 4˚C to 30˚C

Below 9720% ethanol, 4˚C to 30˚C

20% ethanol, 4˚C to 30˚C

Median particle size of the cumulative volume distribution
Samples were applied at 75 cm/h until 50% breakthrough; columns: 0.5 × 5 cm; buffers: 0.05 M Tris, 2 M NaCl (in the elution buffer), pH 7.5 (DEAE) or 0.1 M acetate, 2 M NaCl (in the elution buffer), pH 5.0 (SP and CM)
The pressure/flow characteristics describe the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
4
pH range where resin can be operated without significant change in function
5
pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function
6
pH range where ligand is fully charged; although the ligand is fully charged throughout the range stated, only use the resin within the stated stability range.
7
1.0 M NaOH should only be used for cleaning purposes.
8
Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of 300 cm/h in a Tricorn 5/100 at 10 cm bed height (2 min residence time) for BSA in 50mM Tris, pH 8.0
9D ynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of 300 cm/h in a XK 16/20 column at 13 cm bed height (2.6 min residence time) for thyroglobulin in
0.05 M Tris with 1 M NaCl, pH 7.5
10
Note: The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose 4 Fast Flow (high sub). The difference is the length of the carbon chain of the charged group.
1
2
3

2

All resins are chemically robust and withstand rigorous CIP andsanitization procedures (see “Chemical stability”). Their functional
ion exchange groups are attached to the matrices via chemicallystable ether linkages.
The main resin characteristics are listed in Table 1. Figure 2 shows
the pH working ranges of the ion exchangers as titration curves(using sodium hydroxide), that is, the pH ranges over which thefunctional groups are charged.
(A) 12

pH

10
8
6
4
0

(B) 12

pH

10
8
6
4
0

(C) 12

pH

10
8
6
4
0

(D) 12

pH

10
8
6
4
0

(E) 1210pH

8
6

Packing in laboratory columns
Sepharose Fast Flow resins are supplied in laboratory packs forusers who prefer the flexibility of packing columns of their choice.
Straightforward and well-proven recommendations for packing,operation, and maintenance are included in the instructions
supplied with each pack.

Prepacked Sepharose Fast Flow ion
exchange columns
By providing extra speed, convenience, and reproducibility,
prepacked formats extend the usefulness of Sepharose
Fast Flow ion exchangers. In addition, ÄKTA™ chromatographysystems include preset method templates for the prepacked
columns, which further simplify operation, improve reproducibility,
and save time.
The resins are supplied in four types of columns: HiPrep™ 16/10(20 mL), HiScreen™ (4.7 mL), HiTrap™ (1 mL and 5 mL) andPreDictor™ RoboColumn™ units (200 μL and 600 μL). The five
HiTrap 1 mL columns are also included in HiTrap IEX Selection Kit
together with SP Sepharose XL and Q Sepharose XL. Q Sepharose
Fast Flow and SP Sepharose Fast Flow are also available in theprefilled PreDictor 96-well filter plates for fast and easy processdevelopment. These plates are filled with either 6 μL, 20 μL, or
50 μL chromatography resin.

HiPrep 16/10 columns
HiPrep SP FF 16/10, HiPrep CM FF 16/10, HiPrep Q FF 16/10, and
HiPrep DEAE FF 16/10 are prepacked, ready-to-use IEX columns
for preparative, small-scale purifications. The columns are simple
to operate and compatible with single pump configurations as wellas ÄKTA systems. Rapid enrichment during the initial capture ofproteins from the start material is a suitable application for any of
the columns named above.
HiPrep 16/10 columns are made of polypropylene, which isbiocompatible with biomolecules. Its bed height of 10 cm gives
a volume of 20 mL. See Table 2 for the main characteristics ofthe HiPrep 16/10 column. Note that HiPrep columns cannot be
opened or repacked.
Table 2. Characteristics of HiPrep 16/10 column

Column volume

20 mL

Column dimensions

1.6 × 10 cm

4

Recommended flow rate

2 to 10 mL/min (60 to 300 cm/h)

2

Maximum flow rate

10 mL/min (300 cm/h)

0

Column hardware pressure limit

0.5 MPa (5 bar, 73 psi)

Fig 2. Titration curves showing the pH ranges over which the functionalgroups are charged. (A) CM Sepharose Fast Flow; (B) SP Sepharose Fast Flow;(C) DEAE Sepharose Fast Flow; (D) Q Sepharose Fast Flow; (E) ANX Sepharose
4 Fast Flow (high sub).

1

1

1

Water at room temperature. Flow rate is determined by v × η < 10 mL/min where v = flow rate andη = viscosity.

3

HiScreen columns

PreDictor 96-well plate and PreDictor RoboColumn

HiScreen columns are part of the process development platform
available from Cytiva and designed for method optimization andparameter screening in packed bed. HiScreen columns havesmall bed volumes (4.7 mL), reducing the cost for sample andbuffer consumption. The prepacked columns give reproducible
results scalable to BioProcess™ columns packed with the same
resins using the same flow velocity.

Q Sepharose Fast Flow and SP Sepharose Fast Flow are available
in prepacked PreDictor plates in three different volumes (6 μL,
20 μL, and 50 μL) or in PreDictor RoboColumns of two different
volumes (200 μL and 600 μL).

HiScreen columns are made of biocompatible polypropylene that
do not interact with biomolecules. The columns can be run withperistaltic pumps or chromatography systems, such as ÄKTAsystems. Table 3 lists the characteristics of HiScreen columns.

PreDictor plates are disposable, 96-well filter plates made ofpolypropylene and polyethylene. Each well is prefilled with thedefined amount of chromatography resin. The plates can beused with centrifugation or vacuum, manually or in automatedrobotic systems. PreDictor plates support high-throughput
process development (HTPD) by allowing parallel screening ofchromatographic conditions.

Note: HiScreen columns cannot be opened or repacked.

PreDictor RoboColumns are prepacked miniaturized
chromatography columns.

Table 3. Characteristics of HiScreen columns

PreDictor RoboColumns are a convenient screening format andare also part of Cytiva’s tools for HTPD. These miniaturized columns
support HTPD using a robotic liquid handling workstation, suchas Freedom EVO™ from Tecan, for fully automated and parallel
chromatographic separations. Perform HTPD work using PreDictor
RoboColumn, alone, or as a complement to PreDictor plates.

Column volume

4.7 mL

Column dimensions

0.77 × 10 cm

Column hardware pressure limit

11

0.8 MPa (8 bar, 117 psi)

 ote: The pressure over the packed bed varies depending on a range of parameters such as theNcharacteristics of the chromatography resin and the column tubing used.

Chemical stability

HiTrap columns
HiTrap SP FF, HiTrap CM FF, HiTrap Q FF, HiTrap DEAE FF, and
HiTrap ANX FF (high sub) are small, affordable, and easy-to-use
prepacked 1 mL or 5 mL IEX columns.
The 1 mL column is often used for method screening to quickly
establish optimized binding and elution conditions in packed
bed. The fast and simple column operation is well-suited forthis role, as well as for small-scale purifications. The larger 5 mLcolumn is an excellent choice for group separation and sample
concentration, and when the purification method has been
established and larger amounts of protein need to be purified.
For quick scale-up of purification, two or three HiTrap columns
can easily be connected in series. Further scale-up can be
conducted on HiPrep 16/10 columns (Fig 3).
HiTrap columns are made of polypropylene, which is biocompatible
with biomolecules. Table 4 lists the characteristics of HiTrap
columns. Note that HiTrap columns cannot be opened or repacked.
Table 4. Characteristics of HiTrap 1 mL and 5 mL columns

Column volumes

1 mL and 5 mL

Column dimensions

0.7 × 2.5 cm (1 mL)
1.6 × 2.5 cm (5 mL)

Recommended flow rate

1 mL/min (1 mL)
5 mL/min (5 mL)

Maximum flow rate1

4 mL/min (1 mL)
20 mL/min (5 mL)

Column hardware pressure limit

0.5 MPa (5 bar, 73 psi)

1

4

Room temperature, aqueous buffers

Good chemical stability allows the use of effective CIP schemesthat result in high recoveries over many purification cycles.
Likewise, it allows regular sanitization to prevent microbial
growth and maintain a high level of hygiene. Thus, both CIP
and sanitization promote good economy and are therefore
key factors to consider when selecting ion exchange resins
and prepacked columns for preparative applications.
For CIP, regular washing with 0.5 to 1.0 M sodium hydroxideshould be sufficient to remove most contaminating material,
although very hydrophobic molecules might bind so tightlythat they need to be eluted with strong detergents or organic
solvents, such as 70% ethanol or 30% isopropanol.
General CIP and sanitization protocols for SP Sepharose Fast Flow,
CM Sepharose Fast Flow, Q Sepharose Fast Flow, DEAE Sepharose
Fast Flow, and ANX Sepharose 4 Fast Flow (high sub) are suppliedwith the resins. Note that specific protocols should be developed
according to the nature and condition of the starting material.

Applications
Sepharose Fast Flow ion exchangers have many applications.
Their speed, reliability, and documented success make thema good choice for preparative protein separations. Separating
protein mixtures early in purification schemes puts the highflow rates and capacities of the columns to very effective use.
The excellent availability of suitable equipment also contributes
to effective use of the columns, especially as ÄKTA systems havemethod templates for HiPrep, HiScreen, and HiTrap columns.

Scaling up five-fold and twenty-fold using different
prepacked Q Sepharose Fast Flow columns
As shown in Figure 3, easy scale-up is a key practical benefit
of working with any Sepharose Fast Flow ion exchanger.
A laboratory protein separation was scaled up first five-fold
and then 20-fold on prepacked HiTrap Q FF and HiPrep Q FF 16/10
columns, respectively, with excellent reproducibility.
1. Conalbumin, 2 mg/mL
2. α-lactalbumin, 4 mg/mL
3. Soy trypsin inhibitor, 6 mg/mL
1 column volume (CV) (A) 1 mL; (B) 5 mL; (C) 20 mL
50 mM Tris-HCl, pH 7.3
50 mM Tris-HCl, 0.5 M NaCl, pH 7.3
0% to 100% elution buffer in 20 CV(A) 20 mL; (B) 100 mL; (C) 400 mL(A) 1 mL/min (150 cm/h); (B) and (C) 5 mL/min (150 cm/h)

Sample:Sample volume:
Start buffer:
Elution buffer:
Gradient:
Flow rate:
(A)

2

mAU
280 nm

Separation of bovine plasma components
on SP Sepharose Fast Flow at laboratory andindustrial scales
Figure 4 shows a protein separation scaled up from laboratory
scale (50 mL sample) to industrial production level (6.5 L sample)
on SP Sepharose Fast Flow, again with high reproducibility.
Analysis of the separated proteins showed no significant
differences in the pattern or purity of the individual peaks ateither scale. Note that the height equivalent to the theoreticalplate (HETP) values are essentially the same for both packed
columns, despite their widely differing sizes.
Column:Sample:Sample volume:
Start buffer:
Elution buffer:
Flow rate:

XK 26/20, 15 cm bed height, 80 mL bed volume
Filtered bovine plasma in 0.1 M sodium acetate, pH 5.2
50 mL sample (25 g/L)
0.1 M sodium acetate, pH 5.2
0.4 M sodium acetate, pH 8.0
530 mL/h (100 cm/h)(A) AA280
280nm
nm

15.0

10.0
10.0

3
10.0

ElutionElutionbufferbuffer

1
5.0

5.0
5.0
Start
Start
bufferbuffer

0.0

0

(B)

5

10

15

20

25

30

Volume (mL)

0.0
0.0

mAU
280 nm
25.0

2

00

20
20

30
30

Time(min)
(min)
Time

20.0

Column:Sample:Sample volume:
Start buffer:
Elution buffer:
Flow rate:

3
15.0
1

10.0

50

(B)

0.0
0

(C)

10
10

20

40

60

80

100

120

140 Volume (mL)

BPG 300/500, 15 cm bed height, 10.5 L bed volume
Filtered bovine plasma in 0.1 M sodium acetate, pH 5.2
6.5 L sample (25 g/L)
0.1 M sodium acetate, pH 5.2
0.4 M sodium acetate, pH 8.0
70 L/h (100 cm/h)

AA280
280nm
nm
10.0
10.0

2

mAU
280 nm25.0

ElutionElutionbufferbuffer
5.0
5.0

20.0

Start
Start
bufferbuffer

3

15.0
1

10.0

0.0
0.0
00

5.0

10
10

20
20

30
30

Time(min)
(min)
Time

0.0
0

100

200

300

400

500 Volume (mL)Fig 3. Five-fold and twenty-fold scale-up on prepacked Q Sepharose Fast Flowcolumns. (A) HiTrap Q FF, 1 mL (0.7 × 2.5 cm); (B) HiTrap Q FF, 5 mL (1.6 × 2.5 cm);(C) HiPrep Q FF 16/10, 20 mL (1.6 × 10 cm).

Fig 4. Scale-up from (A) a laboratory column (bed volume 80 mL) packed withSP Sepharose Fast Flow to (B) a column suitable for industrial production (bedvolume 10.5 L) with retained packing and separation characteristics.

5Effect of pH on the separation of standard proteinson a HiPrep CM FF 16/10 column

Influence of different anion functional groups on
retention times for alkaline phosphatase

In Figure 5, the effect of pH on the separation of standard
proteins on a prepacked HiPrep CM FF 16/10 column is shown.
As can be seen, pH 7.5 gives the most distinct separation.

Not only the elution pH affects the separation profile. As Figure 6
illustrates, different ligands, in this case anion groups, can givesmall but significant differences in retention times. After sample
application, the columns were washed and eluted with the same
linear gradient.

HiPrep CM FF 16/10, 20 mL
10 mg apotransferrin, ribonuclease A, and cytochrome C in 1 mL
CIEX pH 3 to 7.5 BufferPrep kit
0% to 50% B in 300 mL (15 CV) where 50% B = 0.5 M NaCl
10 mL/min (300 cm/h)

Sample:Sample:Buffer:Gradient:
Flow rate:(A) A280 nm

%B

200

50

(A) HiTrap DEAE FF, 1 mL, (B) HiTrap Q FF, 1 mL,
and (C) HiTrap ANX FF (high sub), 1 mL
Sample:2 mL E. coli lysate clarified by centrifugation
Sample application: 2 mL
Start buffer:
20 mM Tris-HCl, pH 7.4
Elution buffer:
20 mM Tris-HCl, 0.5 M NaCl, pH 7.4
Equilibration:
20 mL start buffer
Wash:
10 mL start buffer
Elution:
40 mL, linear gradient, 0% to 100% elution buffer
Flow rate:
1 mL/min (150 cm/h)
Sample:280 nm

(A)

A405

350

0
0

200

Volume (mL)

(B) A280 nm

%B

40.0
300
250

30.0

200
20.0

150
100

Conductivity (mS/cm)

0Absorbance 280 and 405 nm (mAU)

100

10.0

50
0.0

0
0.0

10.0

20.0

30.0

40.0

50.0 Volume (mL)

200

50

280 nm

(B)

A405

100

0

0
0

200

Volume (mL)

(C) A280 nm

%B

40.0
500
30.0

400
300

20.0

200

Conductivity (mS/cm)

Absorbance 280 and 405 nm (mAU)

600

10.0
100
0
0.0

10.0 20.0

30.0 40.0 50.0 Volume (mL)

200
280 nm

(C)

A405

600

50

0

0
0

200

Volume (mL)Fig 5. Separations of standard proteins on HiPrep CM FF 16/10 at:(A) pH 5.0; (B) pH 6.5; (C) pH 7.5.

30.0

400
300

20.0

200

Conductivity (mS/cm)

100

Absorbance 280 and 405 nm (mAU)

40.0
500

10.0
100
0
0.0

10.0

20.0

30.0

40.0

50.0

Volume (mL)Fig 6. Clarified E. coli lysate separated on: (A) HiTrap DEAE FF, 1 mL;(B) HiTrap Q FF, 1 mL; (C) HiTrap ANX FF (high sub), 1 mL. Alkaline
phosphatase activity measured at A405.
6

Process development and scale-up to production
The excellent performance of Sepharose Fast Flow ion exchangers
for laboratory-scale, preparative applications naturally lends
itself to process development and scale-up. The resins are well
supported for this task.

Prepacked formats

Quantity

Product code

HiTrap DEAE FF

5 × 1 mL

17505501

5 × 5 mL

17515401

5 × 1 mL

17505601

5 × 5 mL

17515501

5 × 1 mL

17516201

HiTrap CM FF

As members of the BioProcess family, all ion exchangers aresupported with special services and documentation to facilitate
the development, scale-up, and routine operation of production
applications. Validated manufacture, secure supply, and regulatory
support constitute just part of this package.

HiTrap ANX 4 FF (high sub)Ordering information
Product

Quantity

Product code

Q Sepharose Fast Flow

25 mL

17051010

300 mL17051001

5L

17051004

10 L

17051005

60 L

17051060

25 mL

17072910

300 mL

17072901

5L

17072904

10 L

17072905

60 L

17072960

25 mL

17070910

500 mL

17070901

10 L

17070905

60 L

17070960

25 mL

17071910

500 mL

17071901

10 L

17071905

60 L

17071960

25 mL

17128710

SP Sepharose Fast Flow

DEAE Sepharose Fast Flow

CM Sepharose Fast Flow

ANX Sepharose 4 Fast Flow(high sub)5 × 5 mL

17516301

HiTrap IEX Selection Kit

7 × 1 mL

17600233

PreDictor Q Sepharose
Fast Flow, 6 μL

4 × 96-well plates

28943269

PreDictor Q Sepharose
Fast Flow, 20 μL

4 × 96-well plates

28943270

PreDictor Q Sepharose
Fast Flow, 50 μL

4 × 96-well plates

28943271

PreDictor SP Sepharose
Fast Flow, 6 μL

4 × 96-well plates

28943272

PreDictor SP Sepharose
Fast Flow, 20 μL

4 × 96-well plates

28943273

PreDictor SP Sepharose
Fast Flow, 50 μL

4 × 96-well plates

28943274

PreDictor RoboColumn Q
Sepharose Fast Flow, 200 μL

1 × 8-row columns

28986086

PreDictor RoboColumn SP
Sepharose Fast Flow, 200 μL

1 × 8-row columns

28986087

PreDictor RoboColumn Q
Sepharose Fast Flow, 600 μL

1 × 8-row columns

28986180

PreDictor RoboColumn SP
Sepharose Fast Flow, 600 μL

1 × 8-row columns

28986181

Accessories

Quantity

Product code

1/16" male/Luer female*2

18111251

Tubing connector flangeless/M6 female2

18100368

Tubing connector flangeless/M6 male218101798

Union 1/16" female/M6 male6

18111257

Union M6 female/1/16" male5

18385801

Union luerlock female/M6 female2

18102712

500 mL

17128701

5L

17128704

28401081

17128705

HiTrap/HiPrep, 1/16" maleconnector for ÄKTA systems

8

10 L
60 L

17128760

Stop plug female, 1/16"†

511000464

511000355

Fingertight stop plug, 1/16"‡

Prepacked formats

Quantity

Product code

HiPrep DEAE FF 16/10

1 × 20 mL

28936541

HiPrep CM FF 16/10

1 × 20 mL

28936542

HiPrep Q FF 16/10

1 × 20 mL

28936543

Related literature

Product code

HiPrep SP FF 16/10

1 × 20 mL

28936544

11000421

HiScreen Q FF

1 × 4.7 mL

28950510

Handbook: Ion Exchange Chromatography:
Principles and Methods

HiScreen SP FF

1 × 4.7 mL

28950513

Selection guide: Ion Exchange Columns and Media

18112731

HiScreen DEAE FF

1 × 4.7 mL

28978245

Selection guide: Prepacked chromatography
columns for ÄKTA systems

28931778

HiTrap Q FF

5 × 1 mL

17505301

Data file: AxiChrom columns

28929041

5 × 5 mL

17515601

5 × 1 mL

17505401

5 × 5 mL

17515701

HiTrap SP FF* One connector included in each HiTrap package.
†
Two, five, or seven stop plugs female included in HiTrap packages depending on products.
‡
One fingertight stop plug is connected to the top of each HiTrap column at delivery.

7

cytiva.com/protein-purification
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC
or an affiliate. ÄKTA, BioProcess, HiPrep, HiScreen, HiTrap, PreDictor andSepharose are trademarks of Global Life Sciences Solutions USA LLC
or an affiliate doing business as Cytiva.
Freedom EVO is a registered trademark of Tecan Group Ltd. RoboColumn is atrademark of Atoll GmbH. All other third party trademarks are the property of
their respective owners.
© 2020 Cytiva
All goods and services are sold subject to the terms and conditions of saleof the supplying company operating within the Cytiva business. A copy of
those terms and conditions is available on request. Contact your local Cytiva
representative for the most current information.
For local office contact information, visit cytiva.com/contact

CY13444-20Jul20-DF