HyClone classical media
CELL CULTURE

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F12 Ham’s
Dry powdered media
Code number
SH30004, SH30301

Code number
SH30069, SH30339

mg/Lmmol/L

mg/Lmmol/L

Calcium chloride (anhydrous)

116.611.0507116.611.0507

Copper sulfate-5H2 00.0013

0.00001

0.00130.00001

Ferric nitrate-9H2 00.05

0.00010.050.0001

Ferrous sulfate-7H2 00.417

0.00150.417

0.0015Component description
Inorganic salts

Potassium chloride

311.8

4.1824311.8

4.1824Magnesium chloride (anhydrous)28.610.3005

28.61

0.3005Magnesium sulfate (anhydrous)

48.84

0.405648.840.4056

Sodium chloride

6999.5

119.7724

6999.5

119.7724

Sodium phosphate dibasic (anhydrous)

71.02

0.500371.02

0.5003

Sodium phosphate monobasic H2 062.5

0.452962.50.4529

Zinc sulfate-7H2 00.43

0.00150.43

0.0015

Amino acids
L-Alanine

4.45

0.05

4.45

0.05

L-Arginine-HCl

147.5

0.7002147.50.7002

L-Asparagine-H2 0

7.501

0.05

7.501

0.05

L-Aspartic acid

6.65

0.05

6.65

0.05

L-Cysteine-HCl-H2 017.560.1

17.56

0.1

L-Cystine-2HCl

31.29

0.099931.29

0.0999L-Glutamic acid

7.35

0.05

7.35

0.05

L-Glutamine

365

2.4974365

2.4974Glycine18.75

0.249818.75

0.2498

L-Histidine-HCl-H2 031.48

0.150231.48

0.1502

L-Isoleucine

54.47

0.415354.47

0.4153

L-Leucine59.05

0.450259.05

0.4502

L-Lysine-HCl

91.26

0.499691.26

0.4996

L-Methionine

17.24

0.115517.24

0.1155

L-Phenylalanine

35.48

0.214835.48

0.2148L-Proline

17.25

0.149817.25

0.1498

L-Serine

26.25

0.249826.25

0.2498

L-Threonine

53.45

0.448753.45

0.4487

L-Tryptophan

9.02

0.04429.02

0.0442

L-Tyrosine-2Na-2H2 055.79

0.213655.79

0.2136

L-Valine

52.85

0.451152.85

0.4511

cytiva.com

Code number
SH30004, SH30301

Code number
SH30069, SH30339

mg/L

mmol/L

mg/L

mmol/LD-Biotin

0.00360.00001

0.00360.00001

Calcium D-pantothenate

2.24

0.00472.240.0047

Choline chloride

8.98

0.06438.98

0.0643Folic acid

2.65

0.006

2.650.006

Myo-inositol

12.6

0.069912.60.0699

Niacinamide2.020.0165

2.020.0165

Pyridoxal hydrochloride

0000

Component description
Vitamins

Pyridoxine-HCl

2.030.0099

2.030.0099

Riboflavin0.22

0.00060.22

0.0006

Thiamine-HCl

2.170.0064

2.170.0064

Vitamin B120.68

0.00050.68

0.0005

D-Glucose

3151

17.49

3151

17.49

HEPES

3575

15.002100

Hypoxanthine-2Na

2.70.015

2.70.015

Linoleic acid

0.04

0.00010.04

0.0001DL-α-Lipoic acid

0.105

0.00050.105

0.0005

Phenol red-Na

8.60.0229

8.60.0229

Putrescine-2HCl

0.080.0005

0.080.0005

Sodium pyruvate

550.4999550.4999

Thymidine

0.370.0015

0.370.0015

Add 1200

Add 14.284

Add 1200

Add 14.284

OtherSodium bicarbonate

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CY13877-10Aug20-DF