CDM4MAb
HYCLONE MEDIA AND SUPPLEMENTS

HyClone™ CDM4MAb is a chemically defined and animalderived component-free (ADCF) medium. This regulatoryfriendly medium is developed through the HyClone Metabolic
Pathway Design process (see box) to increase process yields
for the manufacture of antibodies and antibody fragments
for therapeutic use in a variety of engineered hybridoma andmyeloma cell lines.
CDM4MAb medium has been successfully tested in a variety ofculture systems, including T-flasks, shaker flasks, and bioreactors
including fed-batch and perfusion cultures. CDM4MAb medium
does not contain phenol red and is available with or without
L-glutamine and poloxamer 188. CDM4MAb is available in liquidand powder formats in user-friendly packaging (Fig 1).
Key features of CDM4MAb medium include•

Animal-derived component-free•Chemically defined formulation•

Designed for high cell yield and monoclonal antibody(MAb) production•

Allows for direct or sequential adaptation•

Component traceability•

Manufactured according to cGMP guidelines

Specifications•

Does not contain phenol red•

CDM4MAb liquid medium is available with poloxamer 188,
with or without L-glutamine•

CDM4MAb powder medium does not contain L-glutamine,
sodium bicarbonate, or poloxamer 188•

Store medium at 2°C to 8°C, away from light•

Powder medium should be stored protected from moisture
in a tightly sealed container

cytiva.com

Fig 1. CDM4MAb medium is available as liquid or powder in pack sizes suitable
for small-volume cell culture as well as large-scale bioprocessing applications.

Metabolic Pathway Design process

An optimal cell culture process is dependent on a varietyof factors, such as cell line, specific clones, media, andfeeds, as well as processes to maximize viable celldensities and productivity. Our experts in medium designand development know and understand these factors at
the metabolic level. They evaluate each metabolic profile,understanding nutritional demands and waste creation, tomake sure the correct nutrient type and quantity is used
to minimize waste and resultant cell toxicity. Our experts
use their understanding of metabolic pathways to optimize
media for enhanced viable cell densities and productivity.
Once the medium has been optimized using this Metabolic
Pathway Design process, our scientists can help you devise
an effective cell culture strategy using a combination ofmedia and feeds to further enrich productivity and reduce
process inefficiencies.

Suggested preparation

Sequential adaptation

Reconstitution of CDM4MAb powder medium

1. Transfer cells grown in current medium into CDM4MAbmedium at a ratio of 1:1 using a seeding density of
2.0 × 105 cells/mL.

1. While stirring, add 19.55 g/L of CDM4MAb powder medium
to cell culture-grade water at 90% of final preparation volume.
If your water source is normally cool, it may be useful to adjust
the water temperature. Using warmer room temperaturewater (22°C to 25°C) will improve solubilization time. Mix for
20 min or until dissolved.
2. Add 0.5 g/L poloxamer 188 and 3.2 g/L sodium bicarbonate.
Minimum mixing time: 20 min.
3. Bring vessel to final volume with cell culture-grade water.
Allow solution to mix for 10 to 20 min.
4. Check pH and osmolality. Expected values:• pH 7.0 to 7.4• Osmolality 280 to 320 mOsm/kg
5. Sterile filter into desired container using a 0.2 µm sterile filter.

Preparation notes
CDM4MAb powder medium does not contain L-glutamine.
Recommended concentration: 6 mM.

2. Incubate culture until two population doublings are observed.
Subculture cells by mixing equal volumes of cell suspension in
conditioned medium and fresh CDM4MAb medium (1:1 ratio).
3. Continue to subculture the cells using this method until
the previously used medium is reduced below 0.05%
concentration and cell viability is > 85%.

Cryopreservation
CDM4MAb medium adapted cells can be cryopreserved ina medium consisting of a 1:1 ratio of fresh and conditioned
CDM4MAb medium. To this mixture, add DMSO to a final
concentration of 7.5%.

Quality control testing
Quality control test specifications are listed in Table 1.
Table 1. Test specifications1

Appearance

Clear solution

Osmolality

280 to 320 mOsm/kg

pH

7.0 to 7.4

2. The caps on culture flasks should be loosened and adequate
vessel headspace should be given to provide gas exchange.

Sterility

No growth (bacteria or fungi)

Endotoxin≤ 1.0 EU/mL1

3. Seeding densities should be ~ 2.0 × 10 5 cells/mL. Cells should
typically be subcultured every 2 to 4 days, as necessary.

Application

Growth promotion

Direct adaptation

Custom production

1. Transfer cells grown in current medium directly into
CDM4MAb medium at 2.0 × 105 cells/mL. Note: increasing
the seeding density at the onset of adaptation can improvesuccess ratios.

Formulations and delivery systems can be customized toyour specific process requirements or optimized to maximize
process yields.

2. When viable cell density reaches 1.0 to 1.5 × 106 cells/mL,subculture the cells.

Rapid Response Production (RRP)

General culture recommendations
1. Cultures should be incubated at 37°C in a 5% CO2 environment.

3. Cells should be subcultured every 48 to 96 h.
4. If cell viability drops below 80%, proceed to sequential
adaptation.

2

1

Refer to certificate of analysis for actual results.

Our RRP program manufactures up to 200 L of your custom
prototype formulation within seven working days of your request.
Use our RRP service to expedite the development and testing ofcustom buffers and process liquids for your biopharmaceuticalmanufacturing process.

Table 2. Supplement matrix

Amino
TraceGrowth Hypoxanthine/ ADCF*
ADCF*
acids Vitamins Glucose elements factors
thymidinelipids cholesterol Suitable for
Cell Boost 1
Supplement (R05.2)•

Cell Boost 2
Supplement (R15.4)•

Cell Boost 3
Supplement (JM3.5)

••

•

•

Cell Boost 4
Supplement (PS307)
Cell Boost 5
Supplement (CN-F)

•
•

•
•

•
•

•
•

•
•

Cell Boost 6
Supplement (CN-T)

••

•

•

•

•

Code number•

HEK293
CHOSH30584•

PER.C6™
CHOSH30596

HybridomaMyeloma

SH30825•LS250 supplement
LS1000 supplement••CHO

SH30857•••

Hybridoma
NS0HEK293
CHOSH30865

•

•

•

T-Cells
Hybridoma
NS0HEK293
CHOSH30866•••

NS0SH30554

NS0SH30555* Animal-derived component-free

Related products
Table 2 gives an overview of HyClone supplements.

HyClone Cell Boost kit
Cell Boost™ Process Supplements (100 g each) contain samples
of supplements designed to increase cell productivity in a varietyof cell lines (Table 2). Each supplement is developed through theMetabolic Pathway Design process and is chemically defined andprotein-free with no animal derived components.

HyClone LS250 supplement
LS250 is a chemically defined, animal-derived component-freelipid supplement developed to stimulate cell growth and MAb
production in NS0 cell cultures using traditional hybridoma
serum-free media.

HyClone LS1000 supplement
LS1000 supplement is a chemically defined, animal-derived
component-free lipid supplement developed to stimulate cell
growth and MAb production in NS0 cell cultures using traditional
hybridoma serum-free media.
The supplement is formulated using a proprietary complexing
process for enhanced cholesterol delivery. LS1000 has beensuccessfully tested in a variety of serum-free medium cultures,including HyClone CDM4NS0 and CDM4MAb media.

3Ordering information
CDM4MAb medium is manufactured in homogenous liquid lot
sizes up to 10 000 L and powder lots up to 250 000 L.
ProductSize

HyClone CDM4MAbliquid medium

500 mL bottle

SH30801.01

1000 mL bottle

SH30801.02

5 L bag

SH30801.03

10 L bag

SH30801.04

20 L bag

SH30801.05

50 L bag

SH30801.06

With L-glutamine
With poloxamer 188

HyClone CDM4MAbliquid medium
Without L-glutamine
With poloxamer 188

HyClone CDM4MAbpowder medium
Without L-glutamine
Without poloxamer 188

Size

HyClone Cell Boost kit

6 × 100 g

HyClone LS1000
cholesterol supplement

50 mL bottle

Product code

100 L bag

SH30801.07

200 L bag

SH30801.08

500 L bag

SH30801.09

500 mL bottle

SH30802.01

1000 mL bottle

SH30802.02

5 L bag

SH30802.03

10 L bag

SH30802.04

20 L bag

SH30802.05

50 L bag

SH30802.06

100 L bag

SH30802.07

200 L bag

SH30802.08

500 L bag

SH30802.09

1 × 5 L*SH30800.01

1 × 10 L*

SH30800.02

1 × 50 L*

SH30800.03

1 × 100 L*

SH30800.04

1 × 500 L†

SH30800.05

1 × 1000 L†High-density polyethylene (HDPE) bottle†Polybag/pail*cytiva.com/hyclone
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco
LLC or an affiliate. Cell Boost and HyClone are trademarks of Global Life
Sciences Solutions USA LLC or an affiliate doing business as Cytiva.
PER.C6 is a trademark of Crucell. All other third-party trademarks arethe property of their respective owners.
© 2020 Cytiva
All goods and services are sold subject to the terms and conditions of saleof the supplying company operating within the Cytiva business. A copyof those terms and conditions is available on request. Contact your local
Cytiva representative for the most current information.
For local office contact information, visit cytiva.com/contact

CY12692-08Jul20-DF

Related products

SH30800.06

HyClone LS250
lipid supplement

Product code
SH30890
SH30554.01

100 mL bottle

SH30554.02

500 mL bottle

SH30554.03

1000 mL bottle

SH30554.04

100 mL bottle

SH30555.01

500 mL bottle

SH30555.02

1000 mL bottle

SH30555.03