Faculty of Education, Laboratory for Exercise andEnvironmental Physiology, Assoc Prof. Tatsuro Amano, PhD

Physiological measurements and products-related
interventions during exercise and heat stress in humans【Keywords】

SweatingSkin blood flow

thermoregulation

Heat stroke

Cardiovascular■ Summary
It is well recognized that exercise and heat stress potentiallyinduce hyperthermia and would cause heat-related illness.
Sweating and cutaneous vasodilation are primary heat loss
pathways in humans and thus understanding these functions isrequired for preventing heat-related illness. We have beeninvestigated human physiology especially thermoregulation
during exercise/heat stress in humans in vivo.

■ Details
Typically we measure core and skin temperatures, local
sweat rate, sweat rate per a gland, numbers of activatedsweat glands, ion reabsorption in sweat glands, skin andforearm blood flows, oxygen uptake, heart rate, bloodpressure, and collecting blood samples (and othervariables).
○ Unique technique(s)• We perform iontophoresis and intradermal microdialysis
which are invasive and non-invasive drug delivery
techniques into the skin. By using these methods, wehave been reported mechanisms of sweating during
exercise and heat stress in humans in vivo (see the refs).
• We also evaluate integrative physiological functions inhumans○ Potential applications and future directions• Elucidating in vivo physiological mechanisms in human,evaluating products such as foods and clothing,enhancing human performance during sports andworks, elucidating sweating mechanisms, anddeveloping new devices.

■ Potential collaboration

References• Amano T, Igarashi A, Fujii N, Hiramatsu D, Inoe Y, and Kondo N. β-adrenergic receptor blockade does
not modify non-thermal sweating during static exercise and following muscle ischemia in habitually
trained individuals. Eur J Appl Physiol, accepted.
• Amano T, Fujii N, Inoue Y, and Kondo N. Cutaneous adrenergic nerve blockade attenuates sweating
during incremental exercise in habitually trained men. J Appl Physiol, accepted.
• Amano T, Okushima D, Breese BC, Bailey SJ, Koga S, and Kondo N. Influence of dietary nitrate
supplementation on local sweating and cutaneous vascular responses during exercise in a hotenvironment. Eur J Appl Physiol, 118:1579-1588, 2018.
• Amano T, Fujii N, Kenny GP, Inoue Y, and Kondo N. Do nitric oxide synthase and cyclooxygenase
contribute to sweating response during passive heating in endurance-trained athletes? Physiological
Reports, 5: e13403, 2017.
• Amano T, Shitara Y, Fujii N, Inoue Y, and Kondo N. Evidence for β-adrenergic modulation of sweating
during incremental exercise in habitually trained males. Journal of Applied Physiology, 123: 182-189,2017.
• Amano T, Hirose M, Konishi K, Gerrett N, Ueda H, Kondo N, and Inoue Y. Maximum rate of sweat ionsreabsorption during exercise with regional differences, sex, and exercise training. European Journal ofApplied Physiology, 117: 1317-1327, 2017.
• Amano T, Fujii N, Louie JC, Meade RD, and Kenny GP. Individual variations in nitric oxide synthasedependent sweating in young and older males during exercise in the heat: role of aerobic power.
Physiol Report, 5: e13208, 2017.
• Amano T, Kai S, Nakajima M, Ichinose-Kuwahara T, Gerrett N, Kondo N, and Inoue Y. Sweatingresponses to isometric handgrip exercise and forearm muscle metaboreflex in prepubertal children andelderly. Experimental Physiology, 102:214-227, 2017.
• Amano T, Ishitobi M, Ogura Y, Inoue Y, Koga S, Nishiyasu T, and Kondo N. Effect of stride frequency onthermoregulatory responses during endurance running in distance runners. Journal of Thermal Biology,
61: 61-66, 2016.

• Companies associated with skin care, food, drinks, and any kind of products, as well as working
places in the heat (e.g., factory).
Contact us: Institute for Social Innovation and Cooperation
TEL：025-262-7554 FAX：025-262-7513 E-mail：onestop@adm.niigata-u.ac.jp