Product datasheet

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080
3 Abreviews 656 References 画像数 6

製品の概要
製品名

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

由来種

Goat

ターゲット生物種

Rabbitアプリケーション

適用あり : IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

免疫原

Other Immunogen Type corresponding to Rabbit IgG.

標識

Alexa Fluor® 594. Ex: 590nm, Em: 617nm

製品の特性
製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

バッファー

Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA

精製度

Immunogen affinity purified

特記事項（精製）

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

ポリ /モノ

ポリクローナルアイソタイプ

IgG

特記事項

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher ScientificCompany. The Alexa Fluor® dye included in this product is provided under an intellectual property
license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, thepurchase of this product conveys to the buyer the non-transferable right to use the purchased
product and components of the product only in research conducted by the buyer (whether thebuyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale ofthis product is expressly conditioned on the buyer not using the product or its components, or any
materials made using the product or its components, in any activity to generate revenue, which
may include, but is not limited to use of the product or its components: in manufacturing; (ii) toprovide a service, information, or data in return for payment (iii) for therapeutic, diagnostic orprophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research.
For information on purchasing a license to this product for purposes other than research, contact
Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or1

outlicensing@thermofisher.com.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150080の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション

特記事項

Abreviews

IHC-Fr(1)

Use at an assay dependent concentration.

ICC/IF

1/200 - 1/1000.

ELISA

Use at an assay dependent concentration. Use at an assay
dependent dilution

IHC-P

Flow Cyt(1)

Use at an assay dependent concentration. Use at an assay
dependent dilution
1/2000 - 1/4000.

画像

ICC/IF image of ab6046 stained HeLa cells. The cells were
4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X100 for 5 minutes and then incubated in 1%BSA / 10% normal
donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block
non-specific protein-protein interactions. The cells were thenincubated with the primary antibody (ab6046, 5µg/ml) overnight at+4°C. The secondary antibody (orange) was ab150080 AlexaFluor® 4594 goat anti-rabbir IgG (H+L) used at 2µg/ml for 1h.DAPI
was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay todemonstrate low non-specific binding of the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Goat
Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)2Overlay histogram showing Jurkat cells stained with ab40763 (redline). The cells were fixed with 4% paraformaldehyde (10 min) andthen permeabilized with 0.1% PBS-Tween for 20 min. The cells
were then incubated in 1x PBS / 10% normal goat serum / 0.3M
glycine to block non-specific protein-protein interactions followed by
the antibody (ab40763, 1/1000 dilution) for 30 min at 22°C. The
secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 594)
(ab150080) was used at 1/4000 dilution for 30 min at 22°C. Isotypecontrol antibody (black line) was rabbit IgG (monclonal) (ab172730,0.1μg/1x106 cells) used under the same conditions. Unlabelledsample (blue line) was also used as a control. Acquisition of >5,000
Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexaevents were collected using a 561nm laser and 610/20 bandpass

Fluor® 594) (ab150080)

filter.

Cross-reactivity of the polyclonal secondary antibody ab182016
was tested using a sandwich ELISA approach. The wells werecoated with the indicated IgG standards at 1 µg/ml (50 µl/well) andincubated overnight at 4°C, followed by a 5% BSA blocking step for
2h at RT. ab182016 was then added starting at 1 µg/ml andgradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For
the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used
at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

For the batch tested, ab182016 showed a cross-reactivity of
5-7% towards Human IgG and below 2% towards Mouse IgG,Rat IgG and Chicken IgY.
ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor®
594) (ab150080)

This data was developed using the unconjugated antibody(ab182016).

3

Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat
anti-Rabbit IgG H&L obtained from two different vendors was tested
using a sandwich ELISA approach. The wells were coated with theindicated IgG standards (Rabbit, Human, Mouse and Rat) at 1µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5%
BSA blocking step for 2h at RT. Secondary antibodies were thenadded starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well),followed by incubation for 2h. For the detection Donkey anti-GoatIgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well),followed by incubation for 1h at RT. This data is from a
representative dilution.
ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor®

This data was developed using the unconjugated antibody594) (ab150080)(ab182016).

IHC-P image of alpha smooth muscle actin (ab5694) staining
E16.5 mouse embryo gut. Paraformaldehyde fixed and paraffin
embedded E16.5 mouse embryo gut sections were dewaxed andrehydrated before antigen retrieval (4 mins in a pressure cooker in
10mM Tris/0.4mM EDTA buffer pH 9.5). They were then incubatedin 50mM NH4Cl for 30 minutes and washed/blocked in 3x 10
minute washes of PBS containing 1% BSA + 0.2% gelatine and0.05% saponin. Sections were incubated overnight with a primary
antibody against alpha smooth muscle actin (ab5694), diluted
1/250 in PBS containing 0.1% BSA and 0.3% triton. After 3 x 10
minute washes in of PBS containing 0.1% BSA, 0.2% gelatine andImmunohistochemistry (Formalin/PFA-fixed paraffin-

0.05% saponin, the sections were incubated for 1 hr in theembedded sections) - Goat Anti-Rabbit IgG H&L

secondary antibody (ab150080, diluted 1/400, shown in red) and(Alexa Fluor® 594) (ab150080)

then the 3 washes repeated. Sections were mounted in Vectashield

This image is courtesy of an anonymous abreview.

with DAPI (blue).

4Alexa Fluor® - Goat Anti-Rabbit IgG H&L (AlexaFluor® 594) (ab150080)

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