Pen-type electronic pipette with smartphone APP
Pen-type electronic pipette with wireless communication function
pipetty Smart APP (Dedicated application)

Operation Manual

Operation Manual Ver.

1.0.1

Firmware Ver.

6.0.2

Application Ver.

1.0.1.0

2020.12.28

Index1

Overview ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 1
1.1

About this Operation Manual ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 1

1.2

Notes on using the application ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 1
1.2.1

About application ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 1

1.2.2

About connection ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 1

1.2.3

About Installation ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 1

2

Features ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 2

3

Setup ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 2

4

5
6
7
8

9

3.1

Setting APP permissions ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 2

4.1

How to start

4.2

Startup screen（Signup screen）・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 4

4.3

Main screen ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 5

5.1

Registration of device in Smartphone ・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 6

5.2

Registration of device in APP ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 8

6.1

Registration of Memory ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 11

7.1

How to use Keypad input ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 16

7.2

How to use Voice input ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 18

8.1

Edit ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 19

8.2

Protocol File ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 22

8.3

Modiﬁcation of Protocol File ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 26

9.1

Log File ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 28

Start the program ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 4

・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 4

Connection ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 6
Memory ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 11

Keypad/Voice ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 16
Protocol ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 19

Logging function ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 28

10 Complete the operation ・・・・・・・・・・・・・・・・・・・・・・・・ 29
11 Troubleshooting ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 29©2020 Icomes Lab Co., Ltd.
Icomes Lab, logo mark of Icomes Lab, pipetty and pipetty Pro are registered trademarks of Icomes Lab.
The name "Android", the Android logo, "Google Play" and the Google Play logo are registered trademarks of
Google LLC. In the United States and other countries. Bluetooth Low Energy is a registered trademark ofBluetooth SIG Inc. in the United States and is used by Icomes Lab Inc. under license.
In this Operation Manual, it may be abbreviated as follows.
Bluetooth Low Energy is Bluetooth LE or BLE
Other company names and product names are trademarks or registered trademarks of their respective
companies.
The application speciﬁcations are subject to change without notice.
pipetty-Pro and pipetty Smart have built-in wireless equipment that has received construction design
certiﬁcation as a wireless station for low power data communication systems based on the Radio Law. EYSHCN:
001-A10745

1 Overview
1.1

About this Operation Manual
Please read this Operation Manual before using this product for the ﬁrst time.
We recommend that print this Operation Manual and store it near the product.
This manual is for pipetty Smart or pipetty Pro Android ™ smartphone apps only.
Compatible with ver.1. *. *. * software (application) and ver.PP-6. *. *, ver.PS-6. *. * software (device).

1.2

Notes on using the application
1.2.1 About application【Note】●The log ﬁle is output as the operation result and does not guarantee the dispensed volume.
Depending on the usage conditions, there may be a diﬀerence between the log ﬁle and theactual dispensed volume.
●This application display does not have a tracking function. Therefore, we cannot guarantee thatthe pipetting portion will be wrong due to the work.
● We are not responsible for any trouble or damage caused by using this application.
●The screen images in this manual are examples of use, so the contents may diﬀer depending on
the actual input contents.

1.2.2 About connection【Note】● In connection, when used around a wireless LAN, other wireless devices, around devices that
emit radio waves such as microwave ovens, in places with many obstacles, or in otherenvironments with poor radio wave conditions, the connection may be interrupted frequently, orcommunication may be lost. The speed may be extremely reduced, or an error may occur.
● If you repeatedly charge the battery without using it up, a phenomenon called the “memory
eﬀect” occurs in which the discharge voltage drops, and the connection may be interrupted or
the communication speed may drop even after charging.
●For other precautions regarding wireless communication, please read the pipetty series
Operation Manual.

1.2.3 About Installation
The following system is required for installation.

OS

Android™8.1 ~− 1 −2 Features
The "pipetty Smart App" which is interlocking with a pen-type electric pipette with a wireless communication
function supports reduce researcherʼ s burden and make eﬃcient operation by the reduce time required forswitching between diﬀerence volume pipettes, mode switching, dispensing volume switching and experimentlog saving.

3 Setup
The application can be downloaded from the "Google Play ™" link on our website.
[ URL：https://www.icomes.co.jp/product/pipetty-smart/ ]3.1

Setting APP permissions* It is necessary to turn on the application permission from "Settings" on the smartphone start menu, "Apps andnotiﬁcations" ⇒ "pipettySmartApp.Android" ⇒ "Permissions".

− 2 −Turn on "Storage", "Microphone", and "Location" in APP permission screen.
After completing the settings, tap ● "Close" on the smartphone.

●Location●Microphone●Storage− 3 −➡ Authority required for BT connection.
➡ Access authority for voice recognition.
➡ Folder creation authority for saving logs.

4 Start the program
4.1

How to start( 1 ) On smartphone
Tap the "pipetty Smart" icon.

4.2

Startup screen (Signup screen)
When you run pipetty SmartApp, the startup screen (Registration screen) shown below is displayed.
Proceed the User registration by inputting information from this startup screen.
User registration is optional, but please be aware that if you do not register the necessary information, we may
not be able to provide the service you desire.

Tap "Registration" to complete registration and move to the "Main" screen.
* Icomes Lab Co., Ltd. handles personal information obtained from customers in compliance with the Personal
Information Protection Law and in accordance with the personal information protection policy.
Regarding protection of personal information, tap "Menu" ⇒ "Terms of Service" to open "About protection of
personal information" on Icomes Lab Website, so be sure to conﬁrm the contents.

− 4 −

4.3

Main screen
Description●When you tap Menu, "Registration information" The "Terms of Service" screen will be displayed.

Registration of device(Section 5.2)
Conﬁrmation ofregistration
information(Section 4.2)

Memory function(Section 6)
Keypad/Voice Input(Section 7)
Protocol Function(Section 8)

Be sure to read the Icomes Lab website.
[Caution] Please use a terminal in the online environment.
URL:
https://www.icomes.co.jp/about/privacypolicy/#protection

Please be sure to read "About the protection of personal information".

− 5 −5 Connection
5.1

Registration of device in Smartphone
In order to use pipetty SmartApp, need to register pipetty device in smartphone.
①Open "Settings" in the start menu of the smartphone.
②Open "Settings" in the start menu of the smartphone.
①②③On the connected terminal (Bluetooth), tap "Pair with new device".
* At this time, turn on the pipetty device (the display screen of the pipetty is ﬂashing for mode selection").
③

− 6 −

④"Nordic̲UART" is displayed as "Available device" on the pairing screen with the new device.
⑤ "Nordic̲UART" Tap to switch to the connected terminal screen, and "Nordic̲UART" is displayed in "Currently
connected terminals". (Pairing completed)⑥Tap “Nordic̲UART” to switch to the detailed screen of the terminal and “Connect” will be highlighted.
④⑤⑥

Tap Connect⑦⑦Complete the pairing of pipetty and smartphone by "Back" or "Close" on the smartphone.

− 7 −

5.2

Registration of device in APP
Register the paired device in pipetty Smart App.
Register the device (20 µL, 250 µL, 1000 µL) and the application side with same volume.
【Registration procedure (Registration example: pipetty 20µL)】①Tap the setting of 20µL display on the Main screen.
②If it is ﬁrst setting, "Incorrect pipette information" is displayed, so tap "OK".
③Tap the information ﬁeld of the device to register.
①②③④Conﬁrm the MAC address to register from Select a device and tap it.
⑤Conﬁrm the connection preparation (make sure pipetty device is turned on), then tap "Yes".
⑥Conﬁrm that the MAC address is registered in the information ﬁeld.
④

⑤

⑥

− 8 −

【How to conﬁrm pipetty Number】
pipetty device Number can conﬁrm by pressing the DOWN button while pressing the PUSH button
while the mode selection is ﬂashing.
The lower 6 digits of the MAC address are saved in the shipping state.
Also, can check the lower 6 digits of the factory-set MAC address by pressing the UP button whilepressing the PUSH button.
Note: Cannot be displayed while connected to the application.
（Fig.1）【About error messages】●If the connection destination is incorrect① When selecting a connection, if the device is incorrect, the following display will appear. Tap "OK" toreselect.
● If the pipetty is not turned on(2) (3) The following display also appears when the pipetty is turned oﬀ. Turn on the pipetty and tap "OK" toreselect.
①

②

③

− 9 −

【Register multiple pipetty Smart device 】
By registering up to 3 devices that are paired, can switch between capacities, modes, and dispensing volumes.
* Please note that cannot register multiple items with the same capacity device.
After registering 20µL, 250µL and 1000µL respectively, the screen will be as shown below.
①The button color will change when device registration is completed.
②③ When you tap the capacity button and the button turns blue, the operation is ready.
①

②

③

After

Before2− 10 −

6 Memory
Up to 5 types of dispensing settings can be stored in memory.
By storing frequently used dispensing settings in memory, do not need to set every time.

6.1

How to register Memory①Tap "Memory"①②To register from the Memory screen, tap "Edit" to enter the memory No. registration screen in ③.
②③
Memory No.1 〜 5
Select capacity
Dispensing speed (5 steps)
Aspiration speed (5 steps)
Dispensing mode selects
Single-Mode: Dispense at once
Multi-Mode: Continuous dispensing
Discharge volume setting
Single-Mode: Volume at once
Multi-Mode: Volume and number oftimes* [Settings decimal point of dispensing volume]●250μ → 1.0-99.9 can be set. (However, the device display is up to 19.9)● 1000μ → 5.0-99.9 can be set. (Same as above)
Display on the device is up to 19.9, but the application can set and operate up to 99.9 after the decimal point.
− 11 −

④ Tap 2nd to register the dispensing setting in memory No.2
[Registration example: 20 µL, Dispense speed 4, Aspiration speed 4, Muiti-Mode, 1.5 µL, 6 times]⑤Volume 20µL, Dispense speed 4, Aspiration speed 4, select⑥ Muti-Mode④⑤⑥⑦⑧⑨Tap the number of Multi-Mode dispensing volume µL and the number of times Time to input(input example: Dispense 1.5 µL 6 times)⑦⑧⑨− 12 −

⑩After conﬁrming the completion of input, tap “Return” ◀ to complete the dispensing setting registration andreturn to the Memory screen.
⑩

1⑪The registered dispensing volume is displayed, and tap the corresponding memory No.2, The setting volume
will be transferred to the device.
⑪（Fig.1）* Other memory No. inputs can be set from "Edit" as well.

− 13 −

【Error message】● Incorrect memory entry①②③→When the input value of Volume is incorrect①②③④⑤⑥→When the input value is incorrect (Volume × number of times exceeds the speciﬁed value)④⑥⑤− 14 −

●In case of connection error⑦ When the device is not turned on or when the main screen switch (blue) is not turned on⑦− 15 −

7 Keypad/Voice
Keypad input on the screen and voice input (Japanese / English) to set and enter the volume, dispensing mode,and dispensing volume of pipetty.

7.1

How to use Keypad
Voice input ON and JP / EN switchingON

Select capacity of connected devices
Select dispensing mode
Single-Mode: Dispense at once
Multi-Mode: Continuous dispensing
Dispensing volume setting
Single-Mode: Dispense volume at 1 timeMulti-Mode: Dispense volume andnumber of times
Keypad ( numeric input)
Enter the setting[Keypad input registration example: 1000µL, Single-Mode, 500µL]①Tap 1000µL capacity and select Single-Mode in dispensing mode
②When tap the number part of the dispensing volume setting, the number disappear, and can proceed input.
①②− 16 −

* [Settings below the decimal point of dispensing volume]●250μ → 1.0 - 99.9 can be set. (However, the display of device is up to 19.9)●1000μ → 5.0 - 99.9 can be set. (Same as above)
The display on device is up to 19.9, but the application can set and operate up to 99.9 after the decimal point.

③ After inputting a numerical value on the keypad, tap "Send" to transfer to device.
③（ﬁg.1）* Tap the "Back button" on the smartphone to reset the entered information.
[Error messages]● If the keypad value is entered incorrectly① When the input value of Volume is incorrect②When Volume × number of times exceeds the speciﬁed value①②

Input the “Dispense volume”
which does not exceed thespeciﬁed value.

Input the “Dispense
volume × Number of times”
which does not exceed
the speciﬁed value.

− 17 −

7.2

How to use Voice input[Voice input (Japanese)]①Turn on the capacity button from the Main screen②Turn on the VOICE button and tap "JP (Japanese)".
[Input example: Capacity 1000 µL, Multi-mode, 100 µL, when inputting voice for setting 5 times (Japanese)]③Speak "1000 micro" into the microphone of your smartphone.
→The recognized words are displayed as a keypad and the capacity switches to 1000 µL.
①

②

③
ON

ON

Speak while displaying Recognizing

The recognized words are displayed④When you speak "multi", the keypad is displayed, and it switches to Multi-mode.
⑤The setting of the dispensing volume is called “volume” .
⑥When the value is blank, speak the value “100” . When recognized, the dispensing volume in µL is displayed.
④⑥⑤

Will be blank− 18 −

Will be entered

⑦ It can also specify the number of dispenses in the same way.
⑧The numerical value is called "5".
⑨When you say “Send” after completed the input, the data will be transferred to device.
⑦⑧⑨

8 Protocol
8.1

Edit
Create and input protocol ﬁles and edit.
①Tap "Edit" to open the edit screen.
②If there is no target ﬁle for "Save File" and "Delete File", an error will be displayed.
①②− 19 −

③ - ⑤Tap “Add” to add items and create the required protocol.
③④⑤

Tap twice to enter characters⑥ - ⑧Select ” Capacity” and “Mode”⑥⑦⑧* [Select Mode]” Single” → Quantitative dispensing operation “Multi” → Equal volume continuous dispensing operation“Super Multi” → Diﬀerent volume continuous dispensing operation” Mix” → Mixing (Pipetting) operation “None” → No selection (When do not run device)* [Selecting the number of times]
Only "Multi" can select the number of times.
"Mix" repeats aspiration and dispensing while pressing the operation button of device. (One-time operation)− 20 −

⑨ - ⑪Then enter the “Volume (Dispensing volume)” . Single mode is automatically set to once.
⑨⑩⑪* [Settings below the decimal point of dispensing volume]●250μ → 1.0 - 99.9 can be set. (However, the display of device is up to 19.9)●1000μ → 5.0 - 99.9 can be set. (Same as above)
The display on device is up to 19.9, but the application can set and operate up to 99.9 after the decimal point.
⑫Continue tapping “Add” to add necessary items and input.
(* In the case of Super Multi mode, enter the volume to be dispensed into each item once.)⑬After entered the ﬁle name, "Save File" is enabled.
⑫⑬⑭

Tap twice to enter characters− 21 −

⑮Tap "Save File" to complete and save to a ﬁle.
⑯ If the same ﬁle name exists, a conﬁrmation screen for overwriting will be displayed.
OK ⇒ Overwritten
Cancel ⇒ Please enter a new name
⑮

8.2

⑯

File (Call protocol file)Calls the entered protocol ﬁle and transfers it to device. (Example: Protocol 1)①Before opening File, turn on the connection button.
①

Turn on the button of device

Tap Protocol− 22 −

②Tap File ﬁrst. And tap③ "File Name" twice to display ④"Please select a ﬁle".
②③④

Tap twice to enter characters⑤Select the speciﬁed ﬁle and tap “Start” to start the protocol.
Press the PUSH button on device, and after operation in item 1 is complete, check mark will appear in "Done".
⑤⑥⑦− 23 −

⑧If enter only the protocol characters as in No. 4, tap "Done" manually. ⑨A check mark is added and be able toproceed to the next section.
⑩When the last check mark is completed, a PDF ﬁle will be output as a record in the log ﬁle in the smartphone.
* If operation canceled in the middle of the protocol, a PDF ﬁle that records the time until the check mark is
output.
⑧⑨⑩[Display of popup]①If the following message is displayed during protocol operation, press the "PUSH" button on device to turn on.
②When Cancel is selected,③ "Failed to connect device" is displayed and the screen returns to the protocol
screen. If so, start the protocol over again.
①②③

Conﬁrm the device isturned on− 24 −

④If the following message appears while the protocol is operating, and the message⑤ "... mode start failed"appears after tapping "Yes", the device may not in the mode selection state. Set the device to standby state
and try the protocol again.

④

⑤
Flash

（Fig1）

After tapping "OK", put thedevice in the mode standby
state.

[In case communication is interrupted]●If “Incorrect pipette information” or “Failed to connect” is displayed on the application screen, please try again
from Section 5.2 (pipetty Smart registration).
In that case, please remove the battery from device once, and re-insert it.

− 25 −

8.3

Modify the protocol file
When need to modify the item of the called ﬁle, tap Edit and "Tap File Name” twice.
*If tap only once, the screen "Enter ﬁle name" will appear, and the protocol ﬁle will be created. Return with Cancel
and tap twice to try again.

[File modiﬁcation]①Tap Edit.
②Tap the File Name ﬁeld twice.
③Tap the ﬁle name need to modify.
①②③− 26 −

④ The selected protocol is displayed.
⑤Tap twice on the part need to modify. (Example: Volume in Section 7 changed from 160 to 150 µL)⑥Enter the value to be modiﬁed.
④⑤⑥⑦Conﬁrm that the value has been changed, and tap "Save File".
⑧A conﬁrmation message for overwriting the ﬁle will be displayed. OK ⇒ Overwrite.
If set a new ﬁle name, Cancel ⇒ Return, tap the File Name ﬁeld once to enter a new name, and tap OK (⑨ is thecorrection completion screen)⑦⑧⑨− 27 −

9 Logging function
In the logging function mode, basic operations are performed by device, and only the operation results arerecorded in the log.

9.1

Log files
Operation result log is from the shared storage in the smartphone ﬁle.
The execution result log is output as a pdf ﬁle to the shared storage\ Android \ data \ jp.co.icomes.pipettysmartapp \ ﬁles \ PipettySmartAppin the smartphone ﬁle. The following is the output of the operation result by the protocol of 8.2.

Protocol A
No
Protocol1 Dispense 500 micro of Reagent A into the tube
2 Dispense 20 micro of Reagent B
3 Dispense 5 micro of mixed liquid into wells 4times
4 Leave for 1 minute
5 Dispense Reagent C into wells by 100,125,150, 175 micro
6
7
8

Capacity
Mode
Volume Times
1000µ L
Single
500.0
1
20µ L
Single
20.0
1
20µ L
Multi
5.0
4
1000µ L SuperMulti
100.0
11000µ L SuperMulti
125.0
11000µ L SuperMulti
160.0
11000µ L SuperMulti
175.0
1

EndTime2020/06/03 11:17:37
2020/06/03 11:17:51
2020/06/03 11:18:08
2020/06/03 11:18:09
2020/06/03 11:18:15
2020/06/03 11:18:17
2020/06/03 11:18:19
2020/06/03 11:18:26

The following operations are saved as the operation result log.
○ Protocol (Item) ○ Capacity (Device volume) ○ Mode ○ Volume (Dispensing volume)○ Times (Number of times) ○ End Time (Completion recording time)* EndTime is output for the part with the "Done" check mark on protocol operation.
[File storage destination for protocol and memory registration]
Shared storage \ Android \ data \ jp.co.icomes.pipettysmartapp \ ﬁles \ PipettySmartApp.
【Notes】
Depending on the smartphone model, the "Protocol ﬁle", "Memory ﬁle", and "Log ﬁle" may not be viewable fromthe PC. In that case, please restart your smartphone or tablet device.
【Notes】
If an error (Err004) is displayed on device during aspiration, the device has exceeded the normal operating
temperature range, so suspend the operation and place the device in a cool place for a certain period of time orchange the dispensing volume.

− 28 −

10 Complete the operation
After completed the operation, return to main screen and tap button of used capacity to disconnection.

11 Troubleshooting
If the problem cannot be solved by the following method or if any other abnormality occurs, please contact us or
distributor.
ProblemCannot connect to device

Easy to disconnect

Inferred factors

SolutionsNo battery inserted in device

Insert the battery

The device display is not
the mode setting screen

Switch the display of device to the mode setting screen (initial screen)

Low battery

Replace with a charged battery

Battery has memory eﬀect

After discharging the battery on the 1st
scale as a guide, repeat charging 2-3 times.

Battery has reached cycle life

Replace with a new battery

Battery is inserted in
the opposite direction
No location permissions
in Smartphone

Insert the battery correctly
Setting location information
authority of smartphone

Low battery

Replace with a charged battery

Battery has memory eﬀect

After discharging the battery on the 1st
scale as a guide, repeat charging 2-3 times.

Battery has reach

Replace with a new battery

Aﬀected by peripherals

Check if there is any inﬂuenceof other wireless communication− 29 −

Problem

Inferred factors

Solutions

The device and smartphone are
too far apartEasy to disconnect

There is a wall or other shield
between device and the smartphone
The timing to operate is early

Cannot ﬁnd protocol data / log
data

Storage settings are hidden in
Smartphone

Cannot input voice

No microphone authority

Cannot create folder

There is no permission forstorage (Create log)

“Incorrect pipette information” isdisplayed“Connection failed” is displayed

Reduce the distance between device
and the smartphone
Make sure there is no obstruction
between device and smartphone
Operate according to the displayof the application

Show storage settings of Smartphone
Set microphone permission
on Smartphone
Set storage permissions
on Smartphone

Incorrect settings or device
selection

Check the device capacity and MAC
address and try again in Section 5.2.

Device is not turned on

Press the PUSH button to turn on

Low battery

Replace with a charged battery

Problems on the smartphone side

Retry the "pipetty smartphoneregistration" in section 5.1.

Customer Support
Icomes Lab Co., Ltd.
2-4-23, Kitaiioka, Morioka, Iwate, 020-0857 JAPANHealthtech Innovation Hub
TEL: +81-19-601-8228

FAX: +81-19-601-8227

E-mail: globalinfo@icomes.co.jp− 30 −

Website: https://www.icomes.co.jp/en