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Tissue-Tek® Hematoxylin 3G
For Prisma (Plus) standard solution reservoir
Instruction for use
9130-E Solution #1 Solution #2
REF

Intended Purpose
This product is intended for staining nucleus in formalin-fixed andparaffin-embedded (FFPE) specimens or cytopathological specimens. It is
commonly used in conjunction with Eosin (staining cytoplasm), and stained
specimens can be observed for the morphological and structuralcharacteristics of cells under the light microscope.

Intended User• In case of inhalation, immediately cover victim with a blanket, move to freshair and keep quiet. Make victim blow his/her nose and gargle. Seek medical
attention if needed.
• In case of accidental ingestion, give water or salt water to drink, and induce
vomiting. Immediately seek medical advice.
• Immediately call a POISON CENTER/doctor.
• Absorb spillage to prevent material damage.

The intended user is a person who is working in the field of pathology,
anatomy or clinical cytology with basic knowledge of their respective field (e.g.
pathologist, medical laboratory technician) and who is responsible formicroscope slide preparation.

Storage

Test principle

The expiration date is printed on the package.

Nucleus, ribosomes are stained blue by Hematoxylin.

Disposal

How to mix Solution #1 and Solution #2• Outsource disposal of solutions and bottles to a waste company or disposeof them in accordance with applicable local regulations.

M Take one each of Solution #1 and Solution #2 out of the box.
N Add Solution #1 to Solution #2. If Solution #1 is a little left in its bottle, add
Solution #2 a little into the bottle #1 to rinse and put back into the Solution#2.
Attention : Do not grip the pouch too tightly. Doing so may cause thesolution to splash.
O Close the Solution #2 pouch cap firmly. Mix the two solutions well by gentle
inversion approximately 20 times.
Attention : Take care not to spill when mixing the two solutions.
P Record the date of solution preparation on the Solution #2 pouch(Preparation date:), if needed.
Attention : Be sure to close the pouch cap tightly before recording thepreparation date on the pouch.

Materials required but not supplied
Microscope Slides, Xylene, Ethanol, Eosin

Precautions for use• Prepare the staining solution by mixing all of Solution #1 and Solution #2 atroom temperature at least 24 hours before the start of use.
• If the staining solution is used right after preparation, hematoxylin is notoxidized completely, which will result in weak staining.
• Be sure to add all of Solution #1 to Solution #2. Failure to do so will change
the mixture proportion, causing improper staining.
• After mixed, use up within one year and prior to expiration date of theproduct.
• Solution #2 may turn yellow but the change does not affect the staining
quality of the product. Please use as it is.
• Basically, the prepared staining solution should be all placed into thestandard solution reservoir.
• If staining quality or solution volume decreases, replace the staining solution
in use with a newly-prepared one (at least one day after preparation).
• Depending on the use conditions of the staining solution, a differentiation
step may be required.
• If you have any questions or need assistance in using the Hematoxylin 3G,
contact the Sakura distributor or representative.

Safety measures• Confirm the hazard and toxicity information through the Safety Data Sheet(SDS) of the product before use.
• Wear protective gear/eye protection/face protection, as appropriate, whenhandling the product.
• Keep away from heat, hot surfaces, sparks, open flames and other ignition
sources. No smoking.
• Provide adequate ventilation.
• Wash hands thoroughly after handling.
• In case of fire: Use carbon dioxide (CO2), alcohol-resistant foam, dry
chemical or water fog to extinguish.

First aid measures• In case of eye contact, immediately flush the affected eye under running tap
water for at least 15 minutes. Seek medical advice. If eye irritation persists:
Get medical advice/attention.
• In case of skin contact, immediately remove contaminated clothing andshoes, and wash the affected area with plenty of water.

Please visit our website
Sakura Finetek Japan Co., Ltd. www.sakura-finetek.com
Sakura Finetek Europe B.V. www.sakura.com
continuous innovation for pathology• Keep frost-free, in a cool, dry and well-ventilated place (< 30°C). Keep awayfrom oxidizing agents. Protect from sunlight.

Expiration datePrecautions
Warning Solution #1

Danger Solution #2

Solution #1
The above are pictograms for ethanol contained in Solution #1. Contains 10% of components with unknown hazards to the aquatic environment.
• Flammable liquid and vapor• Causes serious eye irritation
Solution #2
The above is a pictogram for Aluminium sulphate, hydrate and Sodium iodate
contained in Solution #2.
• May be corrosive to metals.
• Causes serious eye damage.
• May produce an allergic reaction.

Specifications
ProductContains

Tissue-Tek® Hematoxylin 3G
For Prisma (Plus) standard solution reservoir

Solution #1

24ml

Solution #2

800ml

Active ingredient; Hematoxylin 5 -< 10%Note• The 2D barcode (Tissue-Tek® Prisma barcode) on the pouch label is for use
in the Solution Management with Tissue-Tek Prisma® Plus Automated Slide
Stainer using the 2D barcode reader (option).
• Do not damage the 2D barcode printed on the pouch label. Blurred ordamaged barcodes will result in a read failure.
• If there are any serious incidents that have occurred in relation to the device
shall be reported to the manufacturer and your competent authority.

In vitro diagnostic
medical device

Sakura Finetek Japan Co., Ltd.,
31-1, Nihonbashi-Hamacho 2-chome,Chuo-ku, Tokyo 103-0007 Japan
Sakura Finetek Europe B.V.
Flemingweg 10a
EC REP
2408 AV Alphen aan den Rijn
The Netherlands
MedNet SWISS GmbHCH REP
D4 Platz 4, 6039 Root D4, Switzerland
Sakura Finetek Europe B.V.
Flemingweg 10a2408 AV Alphen aan den Rijn
The Netherlands

CE marking
Keep away from sunlight
30°C

Upper limit of temperature
Fragile, handle with care
Consult instructions for use or
consult electronic instructions for use

Sysmex Suisse AG
Tödistrasse 50, 8810 Horgen,
Switzerland
Made in Japan
2024.03.31

D-21G003-E017-03