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Tissue-Tek® Coverslipping Film
Instruction for use

REF

Tissue-Tek® Coverslipping Film is a film designed to be used onlywith the Sakura Finetek Tissue-Tek Film®.

Intended Purpose
This product is used to cover a specimen on a microscope slide,to serve as a physical barrier from mechanical force orenvironmental exposure. The specimen to be protected can be aformalin-fixed and paraffin-embedded (FFPE) section, frozen
section or cytology sample on a microscope slide. The adhesionis activated by an actuator and once the adhesive has dried, thecoverslipped slides can be used for microscopic evaluation,scanning or transport. This product, if desired, can be removed.
It is a single use device and is used in the Film coverslipper.

Intended User
This product is intended to be used by trained professionals inclinical pathology, anatomy or cytology who are trained using theSakura Finetek Tissue-Tek Film®.

How to use• This product only uses xylene as the actuator.
• This product can be used on both hydrophilic and hydrophobic
glass slides.
• Allow the film to completely acclimate to the environment inwhich it will be used. The film package should be opened toallow the film to acclimate for 24 hours prior to use.
• Discard the first 80 cm of film before installing a new roll on thecoverslipper. This will ensure removal of the blemished area
originally secured with adhesive tape.
• When re-installing, remove 3 to 6 mm of the film using theblade cutter of the instrument.
• At the end of the workday or once you have finished working
for the day, remove the roll from the coverslipper, rewind thefilm onto the roll and store it in the plastic bag to prevent thefilm taking the curvature of the roller(s).
• For film installation and more detail of use, refer to the operating
manual of the device to be used together with this product.
Note: In the final steps of specimen staining, clean, reagentgrade xylene should always be used to prepare slides forcoverslipping.

4770-E

Removal of the Coverslipping Film
This film can be removed according to the following procedure.
1. Dip a film coverslipped slide into a minimum of 50 mLacetone for 6 minutes. When processing multiple slides atonce, leave some space between them so that the peeledfilms on the slide do not come in contact with each other.
2. After the slides are soaked in acetone for 6 minutes, remove
the film by using tweezers.
3. Dip slides up and down in 2 changes of xylene, (each 50 mL)
to remove remaining film and adhesive until the white turbidity
on the slide disappears. 50 mL of xylene is sufficient for up to
10 coverslipped slides.
Note: The slide(s) can be re-coverslipped with new film.

Precautions for use• Observe the operating and storage environment as
deterioration of the film may cause coverslipping errors.
• Do not use film when stored out of specifications.
• Please confirm if the surface of the slide is dried out before use
in a digital slide scanner.
• Inspect slides to reduce and/or limit thickness variations.
Cytology specimens may present specimen thickness that
may vary within wide extremes. Bubbles may occur and renderspecimens difficult to diagnose after storage.

Safety measures
Wear gloves and a mask to prevent exposure from actuator(xylene).

Operating and storage environment
Operating and storage:
10°C to 30°C (50°F to 86°F), humidity 30% to 70%.
Film coverslipped slide storage (Recommendation):
20°C to 25°C (68°F to 77°F), less than humidity 50%.

Expiration date
The expiration date is printed on the front of 1 case and on topof the 1 roll package.

Disposal
Dispose to an approved waste company or in accordance withapplicable local regulations.

Note
Any serious incidents that occurred in relation to the device shallbe reported to the manufacturer, authorized representative andyour competent authority.

Sakura Finetek Japan Co., Ltd.
31-1, Nihonbashi-Hamacho 2-chome,Chuo-ku, Tokyo 103-0007 Japan

In vitro diagnostic
medical device

Keep away from sunlight

Sakura Finetek Europe B.V.
Flemingweg 10a2408 AV Alphen aan den Rijn
The Netherlands

Consult instructions for use

EC REP

Keep dry

CH REP

MedNet SWISS GmbH
D4 Platz 4, 6039 Root D4, Switzerland
Sakura Finetek Europe B.V.
Flemingweg 10a2408 AV Alphen aan den Rijn
The Netherlands
Sysmex Suisse AG
Tödistrasse 50, 8810 Horgen,
Switzerland
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continuous innovation for pathology

Fragile, handle with care

30°C
10°C

Temperature limit70%

CE marking

Humidity limitation
Do not re-use

30%

Please visit our website
Sakura Finetek Japan Co., Ltd. www.sakura-finetek.com
Sakura Finetek Europe B.V. www.sakura.com
2024.3.14

D-23H003-I016-02