POLYAMINES 2000
BIOLOGICAL AND CLINICAL PERSPECTIVES
2ND EUROPIAN POLYAMINE CONFERENCE
Program★
Plenary Lecture
Chairman: C. M. Caldarera (Italy)
A. E. Pegg (USA):
Polyamines: the past, present and future.

Session 1: Polyamines, Growth, Senescence and Apoptosis
Chairman: H. M. Wallace (UK)[C 1.1] H. M. Wallace, J. Keen, A. V. Fraser, L. Denby, K. Thompson, G. S.
Lindsay (UK):The role of polyamines in apoptosis.
[C1.2] M. A. Desiderio, E. Matteucci (Italy):
Growth arrest and apoptosis in tumor cells are correlated with antizyme
induction.
[C1.3] L. R. Johnson, M. J. Viar, R. Ray, L. M. Pfeffer (USA):
Polyamine depletion prevents apoptosis in intestinal epithelial cells, in part by
an NF-k dependent mechanism.
[C 1.4] C. Stefanelli, C. Mackintosh, I. Stanic, F. Bonavita, A. Facchini, M.
Fattori, C. Pignatti, B. Tantini, F. Flamigni, C. Clo, A. E. Pegg, C. M.
Caldarera (Italy/USA):
Polyamines and caspase activation.
[C1.5] C. Hanfrey, C. Illingworth, E. Morlon, M. Mayer, A. Dale, S.
Ellwood, A. J. Michael (UK):
Translational regulation of the Arabidopsis S-adenosylmethionine
decarboxylase.

[C1.6] M. M. Altamura, G. Falasca, M. Possenti, G. Tronelli, D. Zaghi, F.
Capitani, N. Bagni (Italy):Interaction between calcium and putrescine in the control of organogenesis
from tobacco thin layers and leaf explants.

Session 2: Polyamines, transport and signal transduction
Chairman: O. Heby (Sweden)[C 2.1] U. Bachrach (Israel):
Regulation of ornithine decarboxylase expression.
[C 2.2] A. Facchini, B. Zanella, B. Tantini, E. Giordano, C. Steranelli, C. M.
Caldarera, F. Flamigni (Italy):
Opposite effects of P44/42 MAPK and P38 MAPK inhibitors on the inductionof ornithine decarboxylase.
[C 2.3] O. Erez, C. Kahana (Israel):
Isolation of a new protein kinase that acts as a key regulator of polyaminetransport.
[C 2.4] K. Igarashi, K. Sakata, A. Kuraishi, H. Tomitori, K. Kashiwagi(Japan):Polyamine transport in Escherichia coli and animal cells.
[C 2.5] E. Morlon, A. J. Michael (UK):
Isolation of two protein kinases from Arabidopsis by functional
complementation of a yeast polyamine transport mutant.
[C 2.6] H. Tomitori, K. Kashiwagi, T. Asakawa, Y. Kakinuma, A. J. Michael,
K. Igarashi (Japan):
Multiple polyamine transport systems on the vacuolar membrane in yeast.

Session 3: Polyamine Metabolism
Chairman: G. Dandrifosse (Belgium)[C 3.0] G. Dandrifosse (Belgium):(Introduction) Importance of polyamine metabolism in cellular life.
[C 3.1] P. Coffino (USA):
Control of polyamine metabolism by means of targeted protein degradation.[C 3.2] J. L. A. Mitchell, G. G. Judd, S. Moore, C. DeBlecourt (USA):On the control of antizme processing, cellular location and activity.
[C 3.3] L. Persson, T. Koichi, E. Lovkvist Wallstrom, S. Nazisadeh, L.
Thiman (Sweden):Translational control of ornithine decarboxylase.
[C 3.4] E. Masini, R. Federico, O. Befani, M. A. Mateescu, J. F. Ndisang, M.
Moncini, P. F. Mannaioni, B. Mondovi (Italy/Canada):
The effect of pea seedling diamine oxidase on guinea pig anaphylaxis.
[C 3.5] K. Kashiwagi, S. Hayashi, T. Fukuchi-Shimogori, K. Nishimura, K.
Igarashi (Japan):
Translational regulation of mRNA having GC-rich 5'-untranslated region ofODC mRNA.
[C 3.6] O. Lepri, E. Hitchin, G. El-Hussieni, L. Bassie, P. Christou, T. Capell(UK):
Putrescine and spermidine accumulation in different rice genotypes
overexpressing the human ornithine decarboxylase cDNA.
[C 3.7] M. T. Olmo, D. Rodriguez-Agudo, C. Rodriguez-Caso, J. L. Urdiales,
M. A. Medina, F. Sanchez-Jimenez (Spain):
Structure/activity and structure/stability relationship studies on recombinant
rat histidine decarboxylase.
[C 3.8] M. Pietila, J. Parkkinen, M. Halmekyto, L. Alhonen, J. Janne(Finland):Mice over-expressing SSAT have similar hairless phenotype and similar
response to retionic acid as the rhino (HRrh) strain.
[C 3.9] M. C. Bastida, A. Cremades, F. Tejada, R. Penafiel (Sweden):
Ornithine decarboxylase and steroidogenesis: influence of aminoglutethimideand DFMO.
[C 3.10] M. Halmekyto, M. Pietila, T. Pirttila, V.-P. Korhonen, L. Alhonen, J.
Janne (Finland):Characterisation of spermidine/spermine N1-acetyltransferase deficient cellline.
[C 3.11] A. Tassoni, M. van Buuren, M. Franceschetti, S. mornale, N.
Bagni (Italy):
Polyamine content and metabolism in Arabidopsis thaliana and effect ofspermidine on plant development.
[C 3.12] S. Soyka, A. G. Heyer (Germany):Differential regulation of Arabidopsis thaliana ADC genes under abiotic
stress.

Session 4: Polyamines and Transglutaminases
Chairman: S. Beninati (Italy)[C 4.1] L. Fesus (Hungary):Induction of transglutaminase in dying cells.
[C 4.2] C. M. Bergamini (Italy):
Structure and stability of tissular transglutaminase in relation to regulation byligands.
[C 4.3] A. Lentini, S. Beninati (Italy):
Theophylline reduces B16-F10 melanoma cells invasion by the enhancementof protein-polyamine cross-links catalyzed by transglutaminase.
[C 4.4] F. Facchiano, D. D'arcangelo, S. Beninati, M. C. Capogrossi (Italy):
Polyamine-induced apoptosis in vascular cells is a transglutaminase-mediatedphenomenon.
[C 4.5] D. Serafini-Fracassini, M. Della Mea, F. Poli, G. Sacchetti, S. Del
Duca, F. Monti, A. M. Bregoli (Italy):Morpho-functional aspects of the Nicotiana tabacum corolla during
development, senescence and programmed cell death.
[C 4.6] R. Ientile, S. Pedale, V. Macaione, V. Torre, S. Macaione (Italy):
Tissue transglutaminase mediates NMDA effects in primary cultures ofcerebellar granule cells.

Session 5: Polyamines and Clinical Aspects
Chairman: A. Corti (Italy)[C 5.1] I. D. Algranati, S. Cejas, M. Cortes, C. Carrillo, N. S. Gonzales(Argentina):
Sensitivity of trypanosomatid parasites to -difluoromethylornithinecorrelates with the metabolic stability of their ornithine decarboxylase.
[C 5.2] R. A. Casero, P. M. Woster (USA):
Terminally alkylated polyamine analogues as chemotherapeutic agents.

[C 5.3] R. G. Schipper, J. C. Romijn, C. M. de Ridder, A. Valasinas, V. K.
Reddy, H. S. Basu, L. J. Marton, B. Frydman, A. A. J. Verhofstad (USA):
Conformationally restricted analogues tetra- and pentamines: growth
inhibitory effects on human prostatic carcinoma cell lines.
[C 5.4] E. Agostinelli, A. Calcabrini, S. Lord-Fontaine, G. Arancia, D. A.
Averill (Italy/Canada):
Cytotoxicity and morphological changes induced by enzymatic oxidation
products of spermine in multidrug resistant cultured cell lines, at 37 and 42 ｡
C.
[C 5.5] B. Martin, F. Posseme, F. Carreaux, B. Carboni, J.-P. Moulinoux, J.-G.
Delcros (France):
In vitro biological evaluation of new boron-containing polyamines for boron
neutron capture therapy.

Session 6: Polyamines and Cancer
Chairman: E. Holtta (Finland)[C 6.1] F. Scorcioni, P. Davalli, S. Astancolle, A. Corti, S. Bettuzzi (Italy):
Simultaneous manipulation of the expression of genes coding for regulatory
proteins of polyamine metabolism resulted in specific alterations of cell cycle
progression in immortalized human prostate epithelial cells.
[C 6.2] V. Milovic, A. R. Khomutov, L. Turchanowa, R. M. Khomutov, W. F.
Caspary, J. Stein (UK/Germany):
Impaired growth of colon cancer cells by hydroxylamine-containing inhibitorsof polyamine biosynthesis and uptake.
[C 6.3] G. Marverti, S. Bettuzzi, M. G. Monti, M. S. Moruzzi (Italy):
Differential transcriptional and post-transcriptional regulation ofspermidine/spermine N1-acetyltransferase activity induced by
N1,N12-bis(ethyl)spermine in cisplatin-sensitive and -resistant cancer cells.
[C 6.4] G. Schillinger, G. Rohn, A. Kleckner, R. Schroder, N. Klug, R. I.
Ernestus (Germany):
ODC-elevation in astrocytomas is regulated posttranscriptionally.
[C 6.5] R. I. Ernestus, G. Rohn, T. Els, G. Schillinger, R. Schroder, N. Klug
(Germany):
Polyamine metabolism in brain tumors: diagnostic significance of quantitativebiochemistry.
[C 6.6] G. Rohn, A. Klekner, G. Schillinger, R. Schroder, N. Klug, R. I.
Ernestus (Germany):
Regulation of ornithine decarboxylase gene expression in recurrent
meningiomas.

Session 7: Polyamines and Food
Chairman: M. A. Grillo (Italy)[C 7.0] M. A. Grillo (Italy):(Introduction) Biologically active amines and food.
[C 7.1] P. Christou, L. Bassie, E. Hinchin, O. Lepri, G. El-Husseiny, P, Hang,P. Nhgia, T. Capell (UK):
Genetic engineering of the polyamine biosynthetic pathway in cereal grains.
[C 7.2] G. Hajos, A. Sass-Kiss, E. Szerdahelyi (Hungary):
Changes of polyamine content of Tokaj grapes and wines.
[C 7.3] L. Colin, L. Geny, M. Brodequis (France):
Evaluation of hydroxycinnamic acid amides during flowering and berries
development of Vitis vinifera.
[C 7.4] S. Biondi, S. Scaramagli, V. Ziosi, P. Torrigiani (Italy):
Modulation of the polyamine-ethylene metabolic interactions in ripening
peach fruits.