PRODUCT INSERT

Dharmacon™ SMARTvector™ lentiviralshRNA pooled libraries: SMARTvector
indexing PCR and sequencing primer
kits A & B
Cat. #PRM7668 & PRM10186

Product description
Dharmacon™ SMARTvector™ Lentiviral shRNA Pooled Libraries are an
efficient and cost-effective method for screening large numbers ofshRNAs without costly automation. SMARTvector library construction,
pooling techniques, and NGS-compatible screening workflows havebeen experimentally validated to ensure reproducibility and accurate hit
identification. SMARTvector libraries are offered for a variety of targeted
gene families as well as druggable genome and whole genome to fit many
experimental designs.

The SMARTvector Indexing PCR and Sequencing Primer Kits A & B containthe following 15 DNA oligonucleotides (See tables for kit-specific part••••

The SMARTvector Lentiviral shRNA Pooled Library screening system
includes Illumina™-adapted PCR primers for identification of shRNA hairpin
sequences from genomic DNA (gDNA) by high-throughput sequencing
on Illumina instrumentation. SMARTvector primer pairs are optimizedfor efficient amplification of shRNA while minimizing thermodynamic
bias and variation in representation. In addition to efficiently amplifying
shRNA hairpins from gDNA, SMARTvector PCR primers have built-inadaptor and index sequences that allow the researcher to easily movefrom PCR amplification to Illumina™ high-throughput sequencing. Direct
identification of shRNA passenger strand facilitates data analysis andensures accurate target gene identification.

dharmacon.horizondiscovery.com

1 × 600 μL tube of SMARTvector Forward PCR Primer
1 × 600 μL tube of each of 12 SMARTvector Reverse Index PCR Primers
1 × 100 μL tube of SMARTvector Read 1 Sequencing Primer
1 × 100 μL tube of SMARTvector Index Read Sequencing Primer

Concentration
Cat. #Description

PRM7668

SMARTvector Indexing PCR and Sequencing Primer Kit A
SMARTvector Forward PCR Primer

PRM10186

Item #

Concentration of oligo solution (μM)

Volume per aliquot (μL)

PRM10211

50600

SMARTvector Reverse Index PCR Primer 2

PRM10188

50600

SMARTvector Reverse Index PCR Primer 4

PRM10190

50600

SMARTvector Reverse Index PCR Primer 5

PRM10191

50600

SMARTvector Reverse Index PCR Primer 6

PRM1019250600

SMARTvector Reverse Index PCR Primer 7

PRM10193

50600

SMARTvector Reverse Index PCR Primer 12

PRM10198

50600

SMARTvector Reverse Index PCR Primer 13

PRM10199

50600

SMARTvector Reverse Index PCR Primer 14

PRM10200

50600

SMARTvector Reverse Index PCR Primer 15

PRM10201

50600

SMARTvector Reverse Index PCR Primer 16

PRM10202

50600

SMARTvector Reverse Index PCR Primer 18

PRM10203

50600

SMARTvector Reverse Index PCR Primer 19

PRM10204

50600

SMARTvector Index Read Sequencing Primer

PRM10214

100100

SMARTvector Read 1 Sequencing Primer

PRM10212

100100SMARTvector Indexing PCR and Sequencing Primer Kit B
SMARTvector Forward PCR Primer

PRM10211

50600

SMARTvector Reverse Index PCR Primer 1

PRM10187

50600

SMARTvector Reverse Index PCR Primer 3

PRM10189

50600

SMARTvector Reverse Index PCR Primer 8

PRM10194

50600

SMARTvector Reverse Index PCR Primer 9

PRM10195

50600

SMARTvector Reverse Index PCR Primer 10

PRM10196

50600

SMARTvector Reverse Index PCR Primer 11

PRM10197

50600

SMARTvector Reverse Index PCR Primer 20

PRM10205

50600

SMARTvector Reverse Index PCR Primer 21

PRM10206

50600

SMARTvector Reverse Index PCR Primer 22

PRM10207

50600

SMARTvector Reverse Index PCR Primer 23

PRM10208

50600

SMARTvector Reverse Index PCR Primer 25

PRM10209

50600

SMARTvector Reverse Index PCR Primer 27

PRM10210

50600

SMARTvector Index Read Sequencing Primer

PRM10214

100100

SMARTvector Read 1 Sequencing Primer

PRM10212

100100

Shipping and storage
Store at –20 °C. The SMARTvector Indexing PCR and Sequencing Primer Kit
is shipped at ambient temperature and should be stored in a -20 °C freezer.
Components provided with this kit are for single use only.

t +44 (0) 1223 976 000 (UK) or +1 800 235 9880 (USA); +1 303 604 9499 (USA)f + 44 (0)1223 655 581
w horizondiscovery.com/contact-us or dharmacon.horizondiscovery.com/service-and-support
Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom
Illumina is a trademark of Illumina, Inc. All trademarks are the property of Horizon Discovery Company unless otherwise specified. ©2018 Horizon
Discovery Group Company—All rights reserved. First published July 2014. UK Registered Head Office: Building 8100, Cambridge Research Park,Cambridge, CB25 9TL, United Kingdom.

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