Test Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 22 00664-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival Date

Approval Date

16-Feb-2022

17-Feb-2022

01-Mar-2022

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Diagnostic Summary
TestColony

Aspiculuris tetraptera PCR

Shikata facility Room 5 (4106)

Tested++/-?

PDG

2100021000Sentinel Mice
Helicobacter hepaticus PCR
Helicobacter hepaticus PCR

Shikata facility Room 6 (4105)
Sentinel Mice+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.

Printed on Mar-01-2022 at 10:50 GMT
Page 1 of 9

Test Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HMResults approved by Kato, Katsuhiko on 01 Mar 2022

Molecular Diagnostics:
Infectious Disease PCR
242526

27

28

2930

31323312345678910

Helicobacter hepaticus PCR----------

Aspiculuris tetraptera PCR----------

Syphacia obvelata PCR----------

34

35

36

37

38

39

40

41

42

43

11

12

13

14

15

16

17

18

1920

Helicobacter hepaticus PCR-+--------

Aspiculuris tetraptera PCR---+------

Syphacia obvelata PCR----------

4445

46

212223

Helicobacter hepaticus PCR---Aspiculuris tetraptera PCR---Syphacia obvelata PCR---Infectious Disease PCR Profile: Helicobacter hepaticus PCR
Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive

Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous
DNA or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for thepurpose of determining the presence of PCR inhibitors.The RNA spike control is also used to evaluate thereverse-transcription of RNA. Amplification of spike template indicates that there is no detectable inhibition and theassay is valid.

Rodentibacter pneumotropicus: (former) P. pneumotropica-Jawetz
Rodentibacter heylii: (former) P. pneumotropica-Heyl
Filobacterium rodentium: (former) CAR Bacillus

Printed on Mar-01-2022 at 10:50 GMT
Page 2 of 9

Test Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OUIbaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM
Results approved by Kato, Katsuhiko on 01 Mar 2022

Serology
12

3

4

5

6

7

8

910

1

2

3

4

5

6

7

8

910

ELISA SEND----------ELISA MHV----------ELISA MPUL----------ELISA CPIL----------ELISA LCMV----------ELISA ECTRO----------MAG C. kutscheri----------MAG S.Typhimurium----------

1112131415161718

1920

111213141516

1718

19

20

ELISA SEND----------ELISA MHV----------ELISA MPUL----------ELISA CPIL--IN-------ELISA LCMV----------ELISA ECTRO----------MAG C. kutscheri----------MAG S.Typhimurium----------IFA CPIL

212223212223

ELISA SEND---ELISA MHV---ELISA MPUL---ELISA CPIL---ELISA LCMV---

ELISA ECTRO---MAG C. kutscheri---

MAG S.Typhimurium---Remarks

Printed on Mar-01-2022 at 10:50 GMT
Page 3 of 9

Test Results

Order #:2022000645
Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 JapanNotes
Shikata facility, Mouse, Serology and PCR testing for routine HM
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction
with tissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by other
serologic assays or by testing of additional samples from the same source; PDG = pending; QNS = Quantity not
sufficient.

FROD: Filobacterium rodentium ((former) CAR bacillus)

Printed on Mar-01-2022 at 10:50 GMT
Page 4 of 9

Test Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Additio

Location InfonUndilutback

Room 2, Sample Type: Serum3 3Mouse

Shikata facility Room 2

ICR

13 weeksFemale(5106) Sentinel
4 4Mouse

Shikata facility Room 2ed
ICR

13 weeks

Female(5106) Sentinel

Undilut

frontedRoom 3, Sample Type: Serum1 1

Mouse

Shikata facility Room 3

ICR

13 weeks

Female(5105) Sentinel
2 2

Mouse

Shikata facility Room 3

Undilut

backedICR

13 weeks

Female(5105) Sentinel

Undilut

frontedRoom 1, Sample Type: Serum5 5

Mouse

Shikata facility Room 1

ICR

13 weeks

Female(5107) Sentinel
6 6

Mouse

Shikata facility Room 1

Undilut

backedICR

13 weeks

Female(5107) Sentinel

Undilut

frontedRoom 4, Sample Type: Serum7 7

Mouse

Shikata facility Room 4

ICR

13 weeks

Female(5115) Sentinel
8 8

Mouse

Shikata facility Room 4

Undilut

backedICR

13 weeks

Female(5115) Sentinel

Undilut

frontedRoom 7, Sample Type: Serum
9 9

Mouse

Shikata facility Room 7

ICR

13 weeks

Female(4104) Sentinel
10 10

Mouse

Shikata facility Room 7

Undilut

backedICR

13 weeks

Female(4104) Sentinel

Undilut

frontedRoom 6, Sample Type: Serum
11 11

Mouse

Shikata facility Room 6ICR

13 weeks

Female(4105) Sentinel
12 12

Mouse

Shikata facility Room 6Undilut

backedICR

13 weeks(4105) Sentinel

FemaleUndiluted

Printed on Mar-01-2022 at 10:50 GMT
Page 5 of 9frontTest Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location Infon

Undilut

backRoom 5, Sample Type: Serum
13 13

Mouse

Shikata facility Room 5

ICR

13 weeks

Female(4106) Sentinel
14 14

Mouse

ed

Shikata facility Room 5

ICR

13 weeks

Female(4106) Sentinel

Undilutfronted

Room 11, Sample Type: Serum
15 15

Mouse

Shikata facility Room 11ICR

13 weeks

Female(4116) Sentinel
16 16

Mouse

Undilut

backedShikata facility Room 11ICR

13 weeks

Female(4116) Sentinel

Undilutfronted

Room 10, Sample Type: Serum
17 17

Mouse

Shikata facility Room 10

ICR

13 weeks

Female(4117) Sentinel
18 18

Mouse

Undilutbacked

Shikata facility Room 10

ICR

13 weeks

Female(4117) Sentinel

Undilut

frontedRoom 9, Sample Type: Serum19 19

Mouse

Shikata facility Room 9

ICR

13 weeks

Female(4118) Sentinel
20 20

Mouse

Undilut

backedShikata facility Room 9

ICR

13 weeks

Female(4118) Sentinel

Undilut

frontedRoom 8, Sample Type: Serum
21 21

Mouse

Shikata facility Room 8

ICR

13 weeks

Female(4119) Sentinel
22 22

Mouse

Undilut

backedShikata facility Room 8

ICR

13 weeks

Female(4119) Sentinel

Undilut

fronted3121, Sample Type: Serum23 23

Mouse

Shikata facility 3121

ICR

13 weeks

SentinelFemale

Undiluted

Room 3, Sample Type: Fecal pellet

Printed on Mar-01-2022 at 10:50 GMT
Page 6 of 9-Test Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location InfonPooledback

Room 3, Sample Type: Fecal pellet (continued)
24 1

Mouse

Shikata facility Room 3

ICR, etc-Female(5105) Sentinel

up to
10:1

25 2

Mouse

Shikata facility Room 3

ICR, etc-Female(5105) Sentinel

Pooledfrontup to
9:1

Room 2, Sample Type: Fecal pellet
26 3

Mouse

Shikata facility Room 2

ICR, etc-Female(5106) Sentinel

Pooledbackup to
10:1

27 4

Mouse

Shikata facility Room 2

ICR, etc-Female(5106) Sentinel

Pooledfrontup to
10:1

Room 1, Sample Type: Fecal pellet
28 5

Mouse

Shikata facility Room 1

ICR, etc-Female(5107) Sentinel

Pooledbackup to
10:1

29 6

Mouse

Shikata facility Room 1

ICR, etc-Female(5107) Sentinel

Pooledfrontup to
10:1

Room 4, Sample Type: Fecal pellet
30 7

Mouse

Shikata facility Room 4

ICR, etc-Female(5115) Sentinel

Pooledbackup to
10:1

31 8

Mouse

Shikata facility Room 4

ICR, etc-(5115) Sentinel

FemalePooledup to
10:1

Room 7, Sample Type: Fecal pellet

Printed on Mar-01-2022 at 10:50 GMT
Page 7 of 9frontTest Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location InfonPooledback

Room 7, Sample Type: Fecal pellet (continued)
32 9

Mouse

Shikata facility Room 7

ICR, etc-Female(4104) Sentinelup to9:1

33 10

Mouse

Shikata facility Room 7

ICR, etc-Female(4104) Sentinel

Pooledfrontup to
10:1

Room 6, Sample Type: Fecal pellet
34 11

Mouse

Shikata facility Room 6

ICR, etc-

Female(4105) Sentinel

Pooledbackup to
10:1

35 12

Mouse

Shikata facility Room 6

ICR, etc-

Female(4105) Sentinel

Pooledfrontup to
10:1

Room 5, Sample Type: Fecal pellet
36 13

Mouse

Shikata facility Room 5

ICR, etc-Female(4106) Sentinel

Pooledbackup to
10:1

37 14

Mouse

Shikata facility Room 5

ICR, etc-

Female(4106) Sentinel

Pooledfrontup to
10:1

Room 11, Sample Type: Fecal pellet
38 15

Mouse

Shikata facility Room 11

ICR, etc-Female(4116) Sentinel

Pooledbackup to
9:1

39 16

Mouse

Shikata facility Room 11

ICR, etc-(4116) Sentinel

FemalePooledup to
9:1

Room 10, Sample Type: Fecal pellet

Printed on Mar-01-2022 at 10:50 GMT
Page 8 of 9frontTest Results

2022000645

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location InfonPooledback

Room 10, Sample Type: Fecal pellet (continued)
40 17

Mouse

Shikata facility Room 10

ICR, etc-Female(4117) Sentinel

up to
9:1

41 18

Mouse

Shikata facility Room 10

ICR, etc-Female(4117) Sentinel

Pooledfrontup to
10:1

Room 9, Sample Type: Fecal pellet
42 19

Mouse

Shikata facility Room 9

ICR, etc-Female(4118) Sentinel

Pooledbackup to10:1

43 20

Mouse

Shikata facility Room 9

ICR, etc-Female(4118) Sentinel

Pooledfrontup to
10:1

Room 8, Sample Type: Fecal pellet
44 21

Mouse

Shikata facility Room 8

ICR, etc-Female(4119) Sentinel

Pooledbackup to
10:1

45 22

Mouse

Shikata facility Room 8

ICR, etc-Female(4119) Sentinel

Pooledfrontup to
10:1

3121, Sample Type: Fecal pellet
46 23

Mouse

Shikata facility 3121

ICR, etc-Sentinel

FemalePooledup to10:1Printed on Mar-01-2022 at 10:50 GMT
Page 9 of 9-Test Results

2022000646

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 22 00664-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival Date

Approval Date

16-Feb-202217-Feb-2022

01-Mar-2022

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM

Diagnostic Summary
TestColony

Tested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.

Printed on Mar-01-2022 at 10:49 GMT
Page 1 of 4

Test Results

Order #:

2022000646
Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM
Results approved by Kato, Katsuhiko on 01 Mar 2022

Molecular Diagnostics:
Infectious Disease PCR

Syphacia muris PCR

56781234----Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive

Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous
DNA or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for thepurpose of determining the presence of PCR inhibitors.The RNA spike control is also used to evaluate thereverse-transcription of RNA. Amplification of spike template indicates that there is no detectable inhibition and theassay is valid.

Rodentibacter pneumotropicus: (former) P. pneumotropica-Jawetz
Rodentibacter heylii: (former) P. pneumotropica-Heyl
Filobacterium rodentium: (former) CAR Bacillus

Printed on Mar-01-2022 at 10:49 GMT
Page 2 of 4

Test Results

Order #:2022000646
Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM
Results approved by Kato, Katsuhiko on 01 Mar 2022

Serology
12341234

ELISA SEND----ELISA SDAV----ELISA MPUL----ELISA CPIL----ELISA HANT----

MAG C. kutscheri----

MAG S.Typhimurium----

MAG B.bronchiseptica----Remarks
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction
with tissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by otherserologic assays or by testing of additional samples from the same source; PDG = pending; QNS = Quantity not
sufficient.

FROD: Filobacterium rodentium ((former) CAR bacillus)

Printed on Mar-01-2022 at 10:49 GMT
Page 3 of 4

Test Results

2022000646

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology and PCR testing for routine HMSample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Additio

Location InfonUndilut-3108, Sample Type: Serum
2 2RatShikata facility 3108

Wistar

14 weeks

FemaleSentineled3107, Sample Type: Serum

1 1RatShikata facility 3107

Wistar

13 weeks

FemaleSentinel

Undilut-ed3119, Sample Type: Serum

3 3RatShikata facility 3119

Wistar15 weeks

FemaleSentinel
4 4RatUndilut-ed

Shikata facility 3119

Wistar16 weeks

Undilut

Monitored

boxFemaleNo pool-Female

Pooled-FemaleSentinel
3107, Sample Type: Fecal pellet5 1Rat

Shikata facility 3107

Wistar-Sentinel
3108, Sample Type: Fecal pellet
6 2RatShikata facility 3108

Wistar, etc-

Sentinelup to7:1
3119, Sample Type: Fecal pellet

7 3RatShikata facility 3119

Wistar-FemaleNo pool-

Wistar-FemaleNo pool

MonitorSentinel
8 4RatShikata facility 3119
Sentinelbox

Printed on Mar-01-2022 at 10:49 GMT
Page 4 of 4