ᚑくᄢቇℂᎿቇ⎇ⓥႎ๔
J. Fac. Sci. Tech., Seikei Univ.
Vol.46 No.2 (2009) pp.99-106
㧔․೎⎇ⓥ⾌ߦଥࠆ⺰ᢥ㧕ࠝ࡝࡯ࡉᴤߩశჇᗵ㉄ൻߦኻߔࠆࠞࡠ࠹ࡁࠗ࠼ߩ㒐ᱛലᨐ㑄↰ ᢥੱ ࡮ේ ▵ሶ Prevention Effect of Carotenoids on the Photo-sensitized Oxidation of Olive Oil
Ayato KANDA*1 and Setsuko HARA *2ABSTRACT㧦Virgin olive oil is known to be oxidized easily, although it contains oleic acid as its main
constituent fatty acid. The susceptibility of virgin olive oil to oxidation was thought to be caused bychlorophyll, which acts as a photo-sensitizer in oils.
In the present work, the oxidation behavior of olive oil to which 0-50 ppm of chlorophyll were added
was investigated and the anti-oxidative activities of carotenoids and tocopherol in olive oil containing
chlorophyll were evaluated under autoxidation and photo-sensitized oxidation.
The results show that, the olive oil was oxidized more or less easily depending on the chrolophyll
content. β-Carotene and astaxanthin inhibited remarkably the photo-sensitized oxidation of olive oils
containing chrolophyll, while tocopherol showed a weak prevention effect on the photo-sensitizedoxidation of olive oil.
Therefore, it was confirmed that carotenoids were effective antioxidants for chlorophyll-containing oils
such as virgin olive oil.
Keywords㧦virgin olive oil, chlorophyll, photo-sensitized oxidation, antioxidant, carotenoids                           (Received Septenmber 17, 2009)ࠢࡠࡠࡈࠖ࡞ߪࡐ࡞ࡈࠖ࡝ࡦⅣߩਛᔃߦ Mg2+߇ࠠ࡟࡯㧝㧚✜ ⺰ ࠻ߒߚಽሶౝ㍲Ⴎߢ޽ࠅ㧘Fig 1 ߦߪࡐ࡞ࡈࠖ࡝ࡦⅣߦ⚿ ⁛․ߥ㚅ࠅߣ㘑๧ࠍᜬߟࠝ࡝࡯ࡉᴤߪ࿾ਛᶏ࿾ᣇߢฎวߒߚቭ⢻ၮ߇⇣ߥࠆࠢࡠࡠࡈࠖ࡞ a㧘b㧘ߩ᭴ㅧࠍ␜ߒߊ߆ࠄⷫߒ߹ࠇ㧘ᦨㄭߢߪᣣᧄߢ߽ஜᐽ㘩᧚ߣߒߡᵈ⋡ߚ‫ޕ‬ߐࠇߡ޿ࠆ‫ߩࡉ࡯࡝ࠝߦ․ޕ‬ᨐታࠍߘߩ߹߹៦ࠅൻቇಣℂ߿ടᾲಣℂࠍ৻ಾⴕࠊߥ޿ࡃ࡯ࠫࡦࠝ࡝࡯ࡉᴤߦߪ㧘ฦ⒳ࡆ࠲ࡒࡦ㘃㧘ࡐ࡝ࡈࠚࡁ࡯࡞㧘ࠬࠢࠕ࡟ࡦߥߤߩ᭽‫ޘ‬ߥ᦭ലᓸ㊂ᚑಽ߇⼾ንߦ฽߹ࠇߡ޿ࠆ‫ࠄࠇߎޕ‬㧘ฦ⒳ࡆ
࠲ࡒࡦ㘃߿ࡐ࡝ࡈࠚࡁ࡯࡞ߪ↢૕ౝߦ߅޿ߡᵴᕈ㉄⚛ߩᖡᓇ㗀ࠍᛥ೙ߔࠆലᨐࠍ߽ߜ㧘⠧ൻ㒐ᱛ߿߇ࠎ੍㒐ߦലᨐ⊛ߢ޽ࠆߎߣ߇ႎ๔ߐࠇߡ޿ࠆ㧝㧕㧕‫ߚ߹ޕ‬㧘ਇ㘻๺⢽⢌㉄ߩਛߢ߽ᦨ߽㉄ൻߐࠇߦߊ޿ࠝ࡟ࠗࡦ㉄ࠍਥ᭴ᚑ⢽⢌㉄ߣߒߡ޿ࠆߚ߼㧘ⴊਛ LDL ࠦ࡟ࠬ࠹ࡠ࡯࡞୯ࠍਅߍ㧘േ⣂⎬ൻ╬ߩⴊᩖ∝࡮ᔃ⤳∛ߥߤࠍ੍㒐ߔࠆߎߣ߇⍮ࠄ

(KI ࠢࡠࡠࡈࠖ࡞ߩ᭴ㅧࠇߡ޿ࠆ㧝  ‫ޕ‬✛㤛⦡㊁⩿߿ᨐታ㧘✛⮺ਛߦߪࠢࡠࡠࡈࠖ࡞ a ߣ b ߇߅ ࠝ࡝࡯ࡉᴤ․᦭ߩ✛㤛⦡ߪ㧘ࠝ࡝࡯ࡉߩታߦሽ࿷ߒߡ㧕޿ࠆ⢽ṁᕈ⦡⚛ߢ޽ࠆࠢࡠࡠࡈࠖ࡞ߦࠃࠆ߽ߩߢ޽ࠆ
 *1 ‛⾰↢๮ℂᎿቇ⑼ഥᚻ
 *2 ‛⾰↢๮ℂᎿቇ⑼ᢎ᝼㧔shara@st.seikei.ac.jp㧕‫ޕ‬ࠃߘ 2㨪3㧦1 ߩഀวߢሽ࿷ߒߡ߅ࠅ㧘⣕⥇࡮Ვ⩶૞↪㧘఺∉ജᒝൻ㧘ࠦ࡟ࠬ࠹ࡠ࡯࡞ᛥ೙૞↪㧘ஜ⢗ஜ⣺૞↪ߥߤߩ૞↪ࠍ߽ߟߎߣ߇ႎ๔ߐࠇߡ޿ࠆ 4㧕‫ޕ‬㧙㧙ߒ߆ߒ㧘ࠢࡠࡠࡈࠖ࡞ߪశჇᗵ೷޽ࠆ޿ߪశჇᗵ‛⾰ࠖ࡞㊂ߩ⇣ߥࠆࠝ࡝࡯ࡉᴤߩ㉄ൻ᜼േࠍㅊ〔ߒߚ‫ࠄߐޕ‬ߣ๭߫ࠇ㧘ਅ⸥ߩࠃ߁ߦ㧘ᴤ⢽ߩ㉄ൻࠍଦㅴߔࠆశჇᗵ

ߦ㧘ࠢࡠࡠࡈࠖ࡞฽᦭㊂ߩዋߥ޿ࠝ࡝࡯ࡉᴤߣࠢࡠࡠࡈ‫ߜࠊߥߔޕ‬㧘ࠢࡠࡠ

ࠖ࡞ࠍ 10ppm ᷝടߒߚࠝ࡝࡯ࡉᴤࠍ↪޿ߡ㧘ࠞࡠ࠹ࡁࠗࡈࠖ࡞ߦశ߇ᒰߚࠆߣࠢࡠࡠࡈࠖ࡞ߪశߩࠛࡀ࡞ࠡ࡯ࠍ

࠼ߩ৻㊀㗄㉄⚛ᶖ෰⢻߅ࠃ߮࡜ࠫࠞ࡞᝝ᝒ૞↪ࠍ࠻ࠦࡈๆ෼ߒߡബ⿠৻㊀㗄⁁ᘒ㧔 Chl*㧕ࠍ⚻ߡਇ቟ቯߥਃ㊀㗄ࠚࡠ࡯࡞ߩ㉄ൻ㒐ᱛലᨐߣᲧセߔࠆߎߣߦࠃࠅ㧘ᴤ⢽ߩ⁁ᘒ㧔 Chl*㧕ߦߥࠆ‫ޕ‬෻ᔕᕈߩ㜞޿ࠢࡠࡠࡈࠖ࡞ಽሶߪ

శჇᗵ㉄ൻߦኻߔࠆ᦭ലߥ㒐ᱛᴺࠍ㐿⊒ߔࠆߎߣࠍ⋡⊛ࠛࡀ࡞ࠡ࡯ࠍઁߩ‛⾰ߦਈ߃቟ቯߥၮᐩ⁁ᘒߦᚯࠆ‫৻ޕ‬ߣߒߚ‫ޕ‬૞↪ࠍ␜ߔߎߣ߽⍮ࠄࠇߡ޿ࠆ5)-7)13ᣇ㧘ࠛࡀ࡞ࠡ࡯ࠍਈ߃ࠄࠇߚ‛⾰ߪൻቇ෻ᔕࠍ⿠ߎߔ‫ޕ‬ߟ߹ࠅ㧘ࠢࡠࡠࡈࠖ࡞߇ബ⿠⁁ᘒ߆ࠄၮᐩ⁁ᘒߦᚯࠆߣ㧞㧚ታ㛎ᣇᴺ߈㧘ࠛࡀ࡞ࠡ࡯ࠍၮᐩ⁁ᘒߩਃ㊀㗄㉄⚛㧔 O2㧕ߦਈ߃㧘

3ᵴᕈߩ㜞޿৻㊀㗄㉄⚛㧔 O2㧕ࠍ↢ߓߐߖࠆ‫৻ޕ‬㊀㗄㉄⚛
1㧝 ⹜ᢱ߅ࠃ߮⹜⮎ߪ㔚ሶ߳ߩⷫ๺ᕈ߇㜞ߊ㧘⢽⾰ࠍ᭴ᚑߔࠆਇ㘻๺⢽⢌㉄⹜ᢱᴤߪᩣᑼળ␠ ,㧙ࠝࠗ࡞ࡒ࡞࠭⵾ ࡈࠖ࡝࠶ࡐ࡮

ߦኻߒߡኈᤃߦઃട෻ᔕࠍ⿠ߎߒ㧘ㆊ㉄ൻ⢽⾰㧔ࡅ࠼ࡠࡌ࡝ࠝ ࡇࡘࠕࠝ࡝࡯ࡉࠝࠗ࡞㧔એਅࠝ࡝࡯ࡉᴤ㧕ࠍ૶ࡍ࡞ࠝࠠࠪ࠼㧘LOOH㧕ࠍ↢ߓࠆ‫ޕ‬ᓥߞߡ㧘৻㊀㗄㉄⚛

↪ߒߚ‫㉄ޕ‬ൻ㒐ᱛ೷ߣߒߡ↪޿ߚǪࠞࡠ࠹ࡦߪ๺శ⚐⮎ߩᶖ෰ߪ㘩ຠߩഠൻ㒐ᱛߦ㊀ⷐߢ޽ࠅ㧘శჇᗵ㉄ൻߦࠃᎿᬺᩣᑼળ␠⵾Ǫࠞࡠ࠹ࡦ㧘ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߪࡗࡑࡂߞߡ↢ߓߚ৻㊀㗄㉄⚛ࠍၮᐩ⁁ᘒߩਃ㊀㗄㉄⚛ߦᚯߔࠢ⊒േᯏࢃ⵾ࡇࡘࠕࠬ࠲ࠝࠗ࡞㧔ࡋࡑ࠻ࠦ࠶ࠞࠬ⿠Ḯ㧕ࠍࠛࡦ࠴ࡖ࡯ߣ๭߫ࠇࠆ৻㊀㗄㉄⚛ᶖ෰೷߇ᵈ⋡ߐࠇ㧘ߘ૶↪ߒ㧘࠻ࠦࡈࠚࡠ࡯࡞ߪᣣᷡࠝࠗ࡝ࠝࠣ࡞࡯ࡊᩣᑼળߩઍ⴫⊛ߥ߽ߩߦࠞࡠ࠹ࡁࠗ࠼߇᜼ߍࠄࠇࠆ‫ޕ‬␠⵾ࡒ࠶ࠢࠬ࠻ࠦࡈࠚࡠ࡯࡞ࠍ૶↪ߒߚ‫ޕ‬ࠞࡠ࠹ࡁࠗ࠼ߪ㤛㧘ᯍ㧘⿒⦡ࠍ๒ߔࠆ⢽ṁᕈ⦡⚛ߣߒߡᐢߊേᬀ‛ߦಽᏓߒ㧘ಽሶਛߦᄙᢙߩ౒ᓎੑ㊀⚿วࠍ ಽᨆ߽ߜ㧘὇ൻ᳓⚛߆ࠄߥࠆࠞࡠ࠹ࡦ㘃ߣ㉄⚛ࠍ฽߻ࠠࠨࡦ ⹜ᢱߩ⚵ᚑಽᨆ࠻ࡈࠖ࡞㘃ߦᄢ೎ߐࠇࠆ‫ޕ‬ઍ⴫⊛ߥࠞࡠ࠹ࡁࠗ࠼ߣߒߡ

⹜ᢱᴤߩ⢽⾰⚵ᚑߪ 10mg/mL ࠢࡠࡠࡎ࡞ࡓṁᶧ 1ǴLߪ㧘ǩ㧘Ǫ㧘ǫࠞࡠ࠹ࡦ㧘࡝ࠦࡍࡦ㧘ࠢ࡝ࡊ࠻ࠠࠨࡦ

ࠍࠢࡠࡑࡠ࠶࠼ߦ⽶⩄ߒ㧘ዷ㐿ṁᇦߦࡌࡦ࠯ࡦ㧦ࠢࡠࡠ࠴ࡦ㧘࡞࠹ࠗࡦ㧘ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߥߤ߇޽ࠆ‫࠹ࡠࠞޕ‬ࡎ࡞ࡓ㧦㈶㉄㧩35㧦15㧦1㧔v/v/v㧕ࠍ↪޿ߡዷ㐿ߒߚᓟ㧘ࡁࠗ࠼ߪ৻㊀㗄㉄⚛߆ࠄࠛࡀ࡞ࠡ࡯ࠍฃߌขࠅ㧘㉄⚛ࠍTLC-FID ᴺߦࠃࠅಽᨆߒߚ‫ߚ߹ޕ‬㧘⹜ᢱᴤߩ⢽⢌㉄⚵ᚑၮᐩ⁁ᘒߩਃ㊀㗄㉄⚛ߦᚯߔߣߣ߽ߦ㧘⥄ࠄ߇ฃߌขߞ

ߪ੍߼ Jham ᴺ 13㧕ߦࠃࠅᴤ⢽ߩ᭴ᚑ⢽⢌㉄ࠍࡔ࠴࡞ࠛࠬ

ߚࠛࡀ࡞ࠡ࡯ߪᾲߣߒߡ᡼಴ߔࠆߎߣߦࠃߞߡ৻㊀㗄㉄⚛

࠹࡞ൻߒߚᓟ㧘ⵝ⟎ߣߒߡ⚿วဳࠠࡖࡇ࡜࡝࡯ࠞ࡜ࡓ

ࠍ‛ℂ⊛ߦᶖ෰ߔࠆ૞↪ࠍ߽ߟߎߣ߇⍮ࠄࠇߡ޿ࠆ

8㧕,9)

‫ޕ‬

߹ߚ㧘ࠞࡠ࠹ࡁࠗ࠼ߦߪ࡜ࠫࠞ࡞ㅪ㎮෻ᔕࠍ஗ᱛߐߖ
ࠆᯏ᭴߽ႎ๔ߐࠇߡ߅ࠅ㧘ਇ㘻๺ᴤ⢽ߩ⥄േ㉄ൻߩㆊ

HR-SS-10㧔0.25mm25m㧕
㧔ା๺ൻቇ㧔ᩣ㧕㧕ߣ᳓⚛Ἳࠗࠝࡦൻᬌ಴ེࠍⵝ஻ߒߚࠟࠬࠢࡠࡑ࠻ࠣ࡜ࡈ GC-18A

ဳ㧔㧔ᩣ㧕ፉᵤ⵾૞ᚲ㧕ࠍ↪޿㧘ࠞ࡜ࡓ᷷ᐲ 200͠ߦ߅޿⒟ߢ↢ߓߚ⢽⾰࡜ࠫࠞ࡞ࠍࠞࡠ࠹ࡁࠗ࠼ߩ౒ᓎੑ㊀⚿วߡ GLC ಽᨆߒߚ‫ޕ‬ߦ࠻࡜࠶ࡊߒߡ㧘ᴤ⢽ߩ⥄േ㉄ൻࠍ㒐ᱛߔࠆ૞↪߽␜ ㆊ㉄ൻ‛ଔߩ᷹ቯߔ10) ⢽⾰ߩ㉄ൻഠൻߩᜰᮡߣߒߡ㧘⢽⾰ߩ⥄േ㉄ൻߦ߅ߌ‫ޕ‬৻⥸ߦਇ㘻๺⢽⾰ߩ࡜ࠫࠞ࡞ㅪ㎮෻ᔕߦࠃࠆ㉄ൻࠍᛥࠆ৻ᰴ㉄ൻ↢ᚑ‛ߢ޽ࠆ⢽⾰ࡅ࠼ࡠࡍ࡞ࠝࠠࠪ࠼㊂ࠍ᷹೙ߔࠆߦߪ㧘ᒝ޿࡜ࠫࠞ࡞᝝ᝒ૞↪ࠍ߽ߜ㧘ᄙߊߩᬀ‛ቯߔࠆᦨ߽ઍ⴫⊛ߥᣇᴺߢ޽ࠅ㧘ࡅ࠼ࡠࡍ࡞ࠝࠠࠪ࠼ߣ

11㧕‫ࠦ࠻ޕ‬࡛࠙ൻࠞ࡝࠙ࡓߩ෻ᔕߦࠃߞߡㆆ㔌ߒߚ࡛࠙⚛ࠍ࠴ࠝ⎫ࡈࠚࡠ࡯࡞ߪࡈࠚࡁ࡯࡞ᕈ᳓㉄ၮࠍ฽߻᭴ㅧࠍ߽ߜ㧘⥄㉄࠽࠻࡝࠙ࡓߢṢቯߒ㧘⢽⾰ 1kg ߦኻߔࠆࡅ࠼ࡠࡍ࡞ࠝേ㉄ൻ෻ᔕߩㅪ㎮Ბ㓏ߢ↢ߓࠆ⢽⾰ࡍ࡞ࠝࠠࠪ࡜ࠫࠞ࡞

ࠠࠪ࠼ߩࡒ࡝ᒰ㊂ᢙ㧔meq/kg㧕ߢ⴫ߔㆊ㉄ൻ‛ଔࠍ㔚૏ߣఝవ⊛ߦ෻ᔕߒߡ࠻ࠦࡈࠚࡠࠠࠪ࡜ࠫࠞ࡞㧔Toc࡮㧕ࠍᏅṢቯᴺ 14㧕ߦࠃࠅ᷹ቯߒߚ‫ޕ‬↢ߓ㧘ߎߩ࠻ࠦࡈࠚࡠࠠࠪ࡜ࠫࠞ࡞ߪ߽߁߭ߣߟߩ⢽⾰ ࠻ࠦࡈࠚࡠ࡯࡞ߩቯ㊂ࡍ࡞ࠝࠠࠪ࡜ࠫࠞ࡞ߣ෻ᔕߒߡ㧘㕖࡜ࠫࠞ࡞ൻߔࠆ‫߹ޕ‬ ⥄േ㉄ൻ⹜㛎ߦ߅ߌࠆ࠻ࠦࡈࠚࡠ࡯࡞㊂ߩ⚻ᤨᄌൻᴤਛ฽߹ࠇߡ޿ࠆ࠻ࠦࡈࠚࡠ࡯࡞߇᦭ലߢ޽ࠆ

ߚ㧘࠻ࠦࡈࠚࡠ࡯࡞ߦߪ৻㊀㗄㉄⚛ߩᶖ෰૞↪߽޽ࠆߎߣ߽ႎ๔ߐࠇߡ޿ࠆ

ߪ HPLC ಽᨆߦࠃࠅㅊ〔ߒߚ‫߅ߥޕ‬㧘ౝㇱᮡḰ‛⾰ߣߒߡ(ᩣ)ࠛ࡯ࠩࠗ⵾ߩ 2㧘2㧘5㧘7㧘8-ࡍࡦ࠲ࡔ࠴࡞-6-ࠢࡠ

12㧕‫ޕ‬

ᧄ⎇ⓥߢߪ㧘ࠢࡠࡠࡈࠖ࡞฽᦭㊂߇శჇᗵ㉄ൻߦ෸߷ࡑࡁ࡯࡞ࠍ↪޿㧘੍߼ᮡḰ⹜ᢱࠍ↪޿ߡ૞ᚑߒߚᬌ㊂✢ߔᓇ㗀ࠍ⺞ߴࠆߚ߼㧘߹ߕ㧘శᾖ኿ਅߦ߅޿ߡࠢࡠࡠࡈ

ߦࠃࠅቯ㊂ߒߚ‫߅ߥޕ‬㧘࿕ቯ⋧ߣߒߡ㗅⋧ࠪ࡝ࠞࠞ࡜ࡓ

㧙㧙

ߢ޽ࠆ Fine SIL5㧔4.6250mm㧕㧘⒖േ⋧ߣߒߡࡋࠠࠨࡦ㧦࠹ࡦߩ㉄ൻ㒐ᱛലᨐࠍᬌ⸛ߒߚ‫ޕ‬2-ࡊࡠࡄࡁ㧙࡞㧩99.2㧦0.8㧔0.7mL/min㧕㧘ᬌ಴ེߣߒߡ

Ⱟశᬌ಴ེ㧔Ex.295nm㧘Em.325nm㧕ࠍ↪޿ߚ‫ޕ‬ Ǫࠞࡠ࠹ࡦߣ࠻ࠦࡈࠚࡠ࡯࡞ߩ౒ሽലᨐߩᬌ⸛

2. 2. 4 Ǫ-ࠞࡠ࠹ࡦߩቯ㊂ శჇᗵ㉄ൻ᧦ઙਅߦ߅ߌࠆǪ-ࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛല ⹜ᢱਛߩǪ-ࠞࡠ࠹ࡦ㊂ߪᭂᄢๆ෼ᵄ㐳ߢ޽ࠆ 464nm

ߦ߅ߌࠆๆశᐲࠍ᷹ቯߒ㧘੍߼૞ᚑߒߚᬌ㊂✢߆ࠄ᳞߼ߚ‫߅ߥޕ‬㧘200ppm એ਄ߩǪ-ࠞࡠ࠹ࡦࠍᷝടߒߚࠝ࡝࡯
ࡉᴤߦߟ޿ߡߪ㧘⹜ᢱ 0.1㨙L ࠍࡋࠠࠨࡦߢ 10㨙L ߦᏗ㉼ߒߚṁᶧࠍ૶↪ߒߡๆశᐲࠍ᷹ቯߒߚ‫ޕ‬ᨐࠍ࠻ࠦࡈࠚࡠ࡯࡞ߣᲧセߔࠆߚ߼ߦ㧘࠻ࠦࡈࠚࡠ࡯࡞
ࠍ㒰෰ߒߚ♖⵾ࠝ࡝࡯ࡉᴤߦࠢࡠࡠࡈࠖ࡞ 10ppm ߣԘ࠻ࠦࡈࠚࡠ࡯࡞ 0㧘250㧘500㧘1000㧘1500㧘2500㧘4500ppm

ᷝടߒߚ࠻ࠦࡈࠚࡠ࡯࡞ᷝട♽㧘ԙǪ-ࠞࡠ࠹ࡦ 0㧘750㧘

1500㧘2400㧘3000㧘4000ppm ࠍᷝടߒߚǪ-ࠞࡠ࠹ࡦᷝട
♽ߦߟ޿ߡ㧘ⰯశἮᾖ኿ਅߢ⥄േ㉄ൻ⹜㛎ࠍⴕߞߚ‫ߐޕ‬ࠄߦ㧘Ǫ-ࠞࡠ࠹ࡦߣ Toc ߩ౒ሽലᨐࠍᬌ⸛ߔࠆߚ߼ߦ㧘 ࠢࡠࡠࡈࠖ࡞ߩ᛽಴ ታ㛎ߢ↪޿ࠆࠢࡠࡠࡈࠖ࡞ߪਅ⸥ߩࠃ߁ߦ㧘ࡎ࠙࡟ࡦ⨲߆ࠄࡋࠠࠨࡦ㧦ࠛ࠲ࡁ࡯࡞㧔3㧦1㧕ṁᶧߦࠃࠅ᛽಴㧘Ԛ࠻ࠦࡈࠚࡠ࡯࡞ 200ppm ߣࠢࡠࡠࡈࠖ࡞ 10ppm ࠍᷝടߒߚ♖⵾ࠝ࡝࡯ࡉᴤߦǪ-ࠞࡠ࠹ࡦࠍ 600㧘1200㧘1800㧘

᳓ᵞ㧘⣕᳓㧘Ớ❗ߒߡ☻᛽಴‛ࠍᓧߚ‫ߦࠄߐޕ‬㧘ṁ಴ṁ

2400㧘3000㧘3600ppm ᷝടߒߚ࠻ࠦࡈࠚࡠ࡯࡞+Ǫ-ࠞࡠᇦߣߒߡࡋࠠࠨࡦ㧦㈶㉄ࠛ࠴࡞㧔5㧦1㧘3㧦1㧘2㧦1㧘1㧦࠹ࡦᷝട♽ߦߟ޿ߡ߽ห᭽ߦ㉄ൻ⹜㛎ࠍⴕߞߚ‫߅ߥޕ‬㧘

1㧕ࠍ↪޿ߚࠪ࡝ࠞࠥ࡞ࠞ࡜ࡓࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ࠍⴕ޿㧘Ყセߩߚ߼ߦ㧘࠻ࠦࡈࠚࡠ࡯࡞ࠍᷝടߖߕߦǪ-ࠞࡠ࠹ࡦࡋࠠࠨࡦ㧦㈶㉄ࠛ࠴࡞㧔2㧦1㧕ṁ಴඙ಽߣߒߡࠢࡠࡠࡈ

ߩߺࠍ 2400㧘3000ppm ᷝടߒߚ♽ߦߟ޿ߡ߽૬ߖߡ⹜㛎ࠖ࡞Ớ❗‛ࠍᓧߚ‫ޕ‬Ớ❗‛߅ࠃ߮⹜ᢱਛߩࠢࡠࡠࡈࠖ࡞ߒߚ‫ޕ‬

฽᦭㊂ߪ‫ⷙޟ‬Ḱᴤ⢽ಽᨆ⹜㛎ᴺ‫⸥ޠ‬タߩᣇᴺ15㧕

ߦၮߠ ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߩ㉄ൻ㒐ᱛലᨐߩᬌ⸛

߈㧘630㧘670㧘710nm ߩๆశᐲ߆ࠄ᳞߼ߚ‫ޕ‬ ࠞࡠ࠹ࡁࠗ࠼ߣߒߡࠕࠬ࠲ࠠࠨࡦ࠴ࡦࠍ 8㧑฽߻ࡗࡑ ࠢࡠࡠࡈࠖ࡞ߩ㉄ൻଦㅴ૞↪ߩᬌ⸛ࡂ⊒േᯏࢃ⵾ࡇࡘࠕࠬ࠲ࠝࠗ࡞㧔ࡋࡑ࠻ࠦ࠶ࠞࠬ⿠Ḯ㧕 ࠝ࡝࡯ࡉᴤߩశ㉄ൻߦ෸߷ߔࠢࡠࡠࡈࠖ࡞฽᦭㊂ߩࠍ↪޿㧘ࠝ࡝࡯ࡉᴤ߅ࠃ߮ࠢࡠࡠࡈࠖ࡞ᷝടࠝ࡝࡯ࡉᴤ

ᓇ㗀ࠍ⺞ߴࠆߚ߼㧘ࠝ࡝࡯ࡉᴤߦ  ߢᓧߚࠢࡠࡠࡈࠖ

ߦኻߒߡࠕࠬ࠲ࠠࠨࡦ࠴ࡦ߇ 800-20000ppm ߦߥࠆࠃ߁

࡞ࠍߘࠇߙࠇ㧘0㧘5㧘10㧘25㧘50ppm ᷝടߒߚ⹜ᢱࠍ⺞ߦᷝടߒߡ㧘 ߣห᭽ߦࠕࠬ࠲ࠠࠨࡦ࠴ࡦߩ㉄ൻ㒐ᱛ⵾ߒ㧘ౝᓘ 9cm ߩࠪࡖ࡯࡟ߦ 40g ౉ࠇ㧘1 ᣣ⚂ 12㨔㧘Ⱟലᨐࠍᬌ⸛ߒߚ‫ޕ‬శἮᾖ኿ਅ(700㨪800LUX)ߦ⟎޿ߡ⥄േ㉄ൻߐߖ㧘⚻ᤨ⊛ߦㆊ㉄ൻ‛ଔ㧘࠻ࠦࡈࠚࡠ࡯࡞㊂㧘ࠢࡠࡠࡈࠖ࡞㊂㧘㧟㧚⚿ᨐߣ⠨ኤࠞࡠ࠹ࡁࠗ࠼㊂ࠍ᷹ቯߒߚ‫ޕ‬ ࠝ࡝࡯ࡉᴤߩ⚵ᚑߣᕈ⁁ Ǫࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐߩᬌ⸛ ᧄ⎇ⓥߢ↪޿ߚࠝ࡝࡯ࡉᴤ㧘ࠢࡠࡠࡈࠖ࡞ᷝടࠝ࡝࡯

 Ǫ-ࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐߦߟ޿ߡ  ߣห᭽ߦਅ

ࡉᴤ㧘♖⵾ࠝ࡝࡯ࡉᴤ߅ࠃ߮ࠢࡠࡠࡈࠖ࡞ᷝട♖⵾ࠝ࡝⸥ߩ⹜㛎ߦࠃࠅᬌ⸛ߒߚ‫߅ߥޕ‬㧘ၮ⾰ߩ㉄ൻᐲߪㆊ㉄ൻ࡯ࡉᴤߩ⢽⢌㉄⚵ᚑߣㆊ㉄ൻ‛ଔ㧘࠻ࠦࡈࠚࡠ࡯࡞㊂㧘‛ଔ㧘࠻ࠦࡈࠚࡠ࡯࡞㊂㧘ࠢࡠࡠࡈࠖ࡞㊂߅ࠃ߮ࠞࡠ࠹

ࠢࡠࡠࡈࠖ࡞㊂߅ࠃ߮ࠞࡠ࠹ࡁࠗ࠼㊂ࠍ 6CDNG ߦ␜ߒ

ࡁࠗ࠼㊂ࠍ⚻ᤨ⊛ߦ᷹ቯߔࠆߎߣߦࠃࠅㅊ〔ߒߚ‫ޕ‬ߚ‫ࡉ࡯࡝ࠝ⵾♖ޕ‬ᴤߦߟ޿ߡߪࠝ࡝࡯ࡉᴤߦ฽߹ࠇߡ޿

 ࠢࡠࡠࡈࠖ࡞ήᷝട♽ߚ࠻ࠦࡈࠚࡠ࡯࡞㧘ࠢࡠࡠࡈࠖ࡞߅ࠃ߮ࠞࡠ࠹ࡁࠗ࠼߇ ࠢࡠࡠࡈࠖ࡞ࠍ߶ߣࠎߤ฽߹ߥ޿ࠝ࡝࡯ࡉᴤߦǪ-ࠞ߶߷ቢోߦ㒰෰ߐࠇߡ޿ࠆߎߣࠍ⏕⹺ߒߚ‫ߚ߹ޕ‬㧘ࠝ࡝ࡠ࠹ࡦࠍ 0㧘600㧘1200㧘3600ppm ᷝടߒߚ⹜ᢱࠍ⺞⵾ߒ࡯ࡉᴤ߅ࠃ߮♖⵾ࠝ࡝࡯ࡉᴤߦኻߒ㧘10ppm ⒟ᐲߩࠢࡠ

ߡ⥄േ㉄ൻ⹜㛎ࠍⴕ޿㧘⥄േ㉄ൻ᧦ઙਅߦ߅ߌࠆǪ-ࠞࡠࡠࡈࠖ࡞ࠍᷝടߒߚ߽ߩࠍ㧘ߘࠇߙࠇࠢࡠࡠࡈࠖ࡞ᷝട

࠹ࡦߩ㉄ൻ㒐ᱛലᨐࠍᬌ⸛ߒߚ‫ޕ‬ࠝ࡝࡯ࡉᴤ߅ࠃ߮♖⵾ࠝ࡝࡯ࡉᴤߣߒߡ↪޿ߚ‫ޕ‬ ࠢࡠࡠࡈࠖ࡞ᷝട♽  ࠢࡠࡠࡈࠖ࡞ࠍࡃ࡯ࠫࡦࠝ࡝࡯ࡉᴤ⋧ᒰ㊂㧔10ppm㧕

ᷝടߒߚࠝ࡝࡯ࡉᴤߦǪ-ࠞࡠ࠹ࡦࠍ 0㧘
600㧘
1200㧘
1800㧘



2400㧘3000㧘3600ppm ߘࠇߙࠇᷝടߒ㧘ⰯశἮᾖ኿ਅߢ

⥄േ㉄ൻ⹜㛎ࠍⴕ޿㧘శჇᗵ㉄ൻ᧦ઙਅߦ߅ߌࠆǪ-ࠞࡠ㧙㧙

6CDNG ↪޿ߚฦ⒳ࠝ࡝࡯ࡉᴤߩ⚵ᚑߣᕈ⁁ࠢࡠࡠࡈࠖ࡞ᷝട♖⵾ࠢࡠࡠࡈࠖ࡞ᷝട

ࠝ࡝࡯ࡉᴤ

ࠝ࡝࡯ࡉᴤ♖⵾ࠝ࡝࡯ࡉᴤ

ࠝ࡝࡯ࡉᴤ⢽⾰⚵ᚑ㧔㧑㧕 TAG

100.0

100.0

100.0

100.0

C16:1

1.9

1.9

3.7

3.7

C16:0

C18:0

⢽⢌㉄⚵ᚑ
㧔㧑㧕

17.8

17.8

2.2

2.2

C18:1

68.7

68.7

C18:3

0.5

0.5

C18:2

ㆊ㉄ൻ‛ଔ㧔meq/kg㧕࠻ࠦࡈࠚࡠ࡯࡞(ppm)

8.9

13.5

ࠞࡠ࠹ࡁࠗ࠼(ppm)

16.6

2.0

2.0

58.2

58.2

8.9

19.1

19.1

16.5

0.6

5.1

169.1

169.1

20.5

50.0

0.8

ࠢࡠࡠࡈࠖ࡞(ppm)

16.6

0.4

0.4

0

10.5

00

10.5

00


 ࠢࡠࡠࡈࠖ࡞ߩ㉄ൻଦㅴ૞↪ߩჇടߦ઻ߞߡㅦ߿߆ߦᷫዋߔࠆߎߣ߇ⷰኤߐࠇߚ‫ࠢޕ‬ ࠝ࡝࡯ࡉᴤߦࠢࡠࡠࡈࠖ࡞ࠍߘࠇߙࠇ㧘0㧘5㧘10㧘25㧘

ࡠࡠࡈࠖ࡞ߦࠃࠆࠝ࡝࡯ࡉᴤߩ㉄ൻଦㅴ૞↪ߦኻߒ㧘࠻

50ppm ᷝടߒߚ⹜ᢱࠍ 2.4 ߩ᧦ઙਅߦ⥄േ㉄ൻߐߖ㧘⚻ࠦࡈࠚࡠ࡯࡞ߪ㉄ൻ㒐ᱛ೷ߣߒߡᯏ⢻ߔࠆ߽ߩߩ㧘㉄ൻᤨ⊛ߦㆊ㉄ൻ‛ଔ㧘࠻ࠦࡈࠚࡠ࡯࡞㊂㧘ࠢࡠࡠࡈࠖ࡞㊂㧘ߩㅴⴕ߇ㅦ޿ࠢࡠࡠࡈࠖ࡞ᷝട♽ߢߪ㧘ᧄታ㛎ߢ↪޿ߚࠞࡠ࠹ࡁࠗ࠼㊂ࠍ᷹ቯߒߚ⚿ᨐ㧘(KI ߦ␜ߒߚࠃ߁ߦ

ࠝ࡝࡯ࡉᴤߦ฽߹ࠇࠆ 170ppm ⒟ᐲߩ㊂ߢߪ㉄ൻࠍലᨐࠝ࡝࡯ࡉᴤߩࠢࡠࡠࡈࠖ࡞฽᦭㊂߇Ⴧടߔࠆߦ઻ߞߡㆊ⊛ߦ㒐ᱛߔࠆߎߣߪߢ߈ߥ߆ߞߚߣ⠨߃ࠄࠇߚ‫ߚ߹ޕ‬㧘㉄ൻ‛ଔ߇㗼⪺ߦ਄᣹ߒߚ‫ޕ‬㘩ຠⴡ↢ᴺߦቯ߼ࠄࠇߡ޿㉄ൻߩㅴⴕߦ઻ߞߡ⹜ᢱߩ✛⦡߇ㅌ⦡ߒ㧘ࠢࡠࡠࡈࠖ࡞

ࠆᴤ⢽฽᦭㘩ຠߩၮḰ୯ߢ޽ࠆ 30meq/kg ߦ㆐ߒߚᤨ㑆㊂ߩ 300h ᓟߩᱷሽ₸ߪ޿ߕࠇߩ⹜ᢱߦ߅޿ߡ߽ 30㨪
40㧑ߢ޽ߞߚߎߣ߆ࠄ㧘శߩᓇ㗀ߦࠃࠆࠢࡠࡠࡈࠖ࡞ߩ

ࠍฦ⹜ᢱߦߟ޿ߡᲧセߔࠆߣ㧘ࠢࡠࡠࡈࠖ࡞ήᷝടߩ⹜
ᢱߢߪ 42h ߢ޽ߞߚߩߦኻߒ㧘ࠢࡠࡠࡈࠖ࡞ 5㧘10㧘25㧘

ಽ⸃߇ⷰኤߐࠇߚ‫ޕ‬

50ppm ᷝട♽ߢߘࠇߙࠇ 10㧘8㧘7㧘5h ߣߥࠅ㧘ᷝട㊂ߎࠇࠄߩ⚿ᨐ߆ࠄ㧘ࠢࡠࡠࡈࠖ࡞ࠍㅢᏱ 10ppm ⒟ᐲ฽

ߩჇടߦ઻ߞߡࠝ࡝࡯ࡉᴤߩ㉄ൻ߇ଦㅴߐࠇߚߎߣ߆ࠄ㧘᦭ߔࠆᏒ⽼ߩࡃ࡯ࠫࡦࠝ࡝࡯ࡉᴤߦ߅޿ߡ㧘ࠢࡠࡠࡈࠖ

ࠢࡠࡠࡈࠖ࡞ߩశჇᗵ૞↪߇㗼⪺ߢ޽ࠆߎߣ߇⏕⹺ߐࠇ࡞ߩశჇᗵ૞↪ࠍ㒐ᱛߔࠆߚ߼ߩ᦭ലߥ㉄ൻ㒐ᱛᴺߩ㐿ߚ‫߅ߥޕ‬㧘౒ሽߔࠆ࠻ࠦࡈࠚࡠ࡯࡞㊂߽ࠢࡠࡠࡈࠖ࡞㊂⊒߇ຠ⾰▤ℂ਄ᔅⷐߢ޽ࠆߎߣ߇್᣿ߒߚ‫ޕ‬ㆊ㉄ൻ‛ଔ䊃䉮䊐䉢䊨䊷䊦ᱷሽ₸䉪䊨䊨䊐䉞䊦ᱷሽ㊂㪌㪇㪇㪈㪇㪇㪋㪇㪇㪏㪇㪍㪇㪊㪇㪇㪉㪇㪇㪈㪇㪇䉪䊨䊨䊐䉞䊦㊂㩿㫇㫇㫄㪀䊃䉮䊐䉢䊨䊷䊦ᱷሽ₸㩿㩼㪀ㆊ㉄ൻ‛ଔ㩿㫄㪼㫈㪆㫂㪾㪀㪌㪇㪍㪇㪋㪇㪋㪇㪊㪇㪉㪇㪉㪇㪈㪇㪇㪇㪇㪇㪉㪇㪇㪋㪇㪇㪇㪉㪇㪇㪋㪇㪇ᤨ㑆㩿㪿㪀ᤨ㑆㩿㪿㪀㪉㪇㪇ᤨ㑆㩿㪿㪀ᤨ㑆㪋㪇㪇䉪䊨䊨䊐䉞䊦ήᷝട䉪䊨䊨䊐䉞䊦㪌㫇㫇㫄䉪䊨䊨䊐䉞䊦㪈㪇㫇㫇㫄䉪䊨䊨䊐䉞䊦㪉㪌㫇㫇㫄㪝㫀㪾㪅㪉 䉥䊥䊷䊑ᴤ䈱శჇᗵ㉄ൻ䈮෸䈿䈜䉪䊨䊨䊐䉞䊦䈱ᓇ㗀㧙㧙䉪䊨䊨䊐䉞䊦㪌㪇㫇㫇㫄 Ǫࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛ૞↪෰ߔࠆ૞↪ߦട߃㧘ㅢᏱߩ㉄ൻ᧦ઙਅߢ⿠ߎࠆ࡜ࠫࠞ࡞ ࠢࡠࡠࡈࠖ࡞ήᷝടࠝ࡝࡯ࡉᴤ߅ࠃ߮ࠢࡠࡠࡈࠖ࡞ᷝ

㉄ൻ෻ᔕߦኻߔࠆ㉄ൻ㒐ᱛ૞↪߽᦭ߔࠆߎߣ߇᣿ࠄ߆ߦ

ടࠝ࡝࡯ࡉᴤߦኻߔࠆǪࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐࠍ⹜ߥߞߚ‫ߚ߹ޕ‬㧘Ǫ-ࠞࡠ࠹ࡦࠍᄙߊ฽᦭ߔࠆ⹜ᢱ߶ߤࠢࡠᢱᴤߩㆊ㉄ൻ‛ଔ߇ 100meq/kg ߦ㆐ߔࠆ߹ߢߩᤨ㑆ࠍǪࡠࡈࠖ࡞ߩᷫዋ߇ᛥ೙ߐࠇࠆߎߣ㧘Ǫࠞࡠ࠹ࡦߩᷫዋ߇ࠞࡠ࠹ࡦήᷝട⹜ᢱࠍ 1.00 ߣߒߡᲧセߒ‫⚿ߩߘޔ‬ᨐࠍᄢ߈޿ߎߣ߇ⷰኤߐࠇߚ‫ߪࠇߎޕ‬శࠛࡀ࡞ࠡ࡯ࠍࠢࡠࡠ

Fig.3 ߦ␜ߒߚ‫࡞ࠖࡈࡠࡠࠢޕ‬ήᷝട♽ߦ߅޿ߡǪࠞࡠࡈࠖ࡞ߦઍࠊߞߡǪࠞࡠ࠹ࡦ߇ฃߌࠆߎߣߦࠃߞߡࠢ

࠹ࡦࠍᷝടߔࠆߣᷝട㊂ߦ઻ߞߡ㉄ൻ߇ᛥ೙ߐࠇ㧘ࡠࡠࡈࠖ࡞ߩశಽ⸃ࠍ㒐ᱛߔࠆߣߣ߽ߦ㧘ࠢࡠࡠࡈࠖ࡞

3600ppm ᷝടߩ႐วߦߪ 100meq/kg ߦ೔㆐ߔࠆᤨ㑆߇ήߦࠃࠆ৻㊀㗄㉄⚛ߩ⊒↢ࠍᛥ೙ߔࠆߎߣߦࠃࠅ㧘ࠝ࡝࡯

ᷝടߩ 6.5 ୚ߦ߹ߢᑧ㐳ߐࠇ㧘ࠢࡠࡠࡈࠖ࡞ߦࠃࠆశჇ

ࡉᴤߩ㉄ൻ߇ㅴⴕߔࠆߩࠍ㒐ᱛߒߡ޿ࠆߣ⠨߃ࠄࠇߚ‫ޕ‬ᗵ૞↪߇߶ߣࠎߤߥ޿♽ߦ߅޿ߡ㧘Ǫࠞࡠ࠹ࡦ߇㉄ൻࠍ߹ߚ㧘(KI ߦࠢࡠࡠࡈࠖ࡞ᷝടࠝ࡝࡯ࡉᴤߩ⺃ዉᦼ㒐ᱛߔࠆലᨐ㧘ߔߥࠊߜ㧘࡜ࠫࠞ࡞ㅪ㎮෻ᔕߦࠃࠆ㉄ൻࠍࠢࡠࡠࡈࠖ࡞ήᷝടࠝ࡝࡯ࡉᴤߩ⺃ዉᦼࠍ 1.00 ߣߒߡࠍᛥ೙ߔࠆߎߣ߇⏕⹺ߐࠇߚ‫ޕ‬߹ߚ㧘ࠢࡠࡠࡈࠖ࡞ 10ppm␜ߒߚ‫ߦ߁ࠃߩߎޕ‬㧘ࠢࡠࡠࡈࠖ࡞ߩᷝടߦࠃߞߡ⺃ዉࠍᷝടߒߚࠝ࡝࡯ࡉᴤߦ߅޿ߡ߽㧘Ǫ-ࠞࡠ࠹ࡦߩᷝടߪ

ᦼߪ 0.26 ߹ߢ⍴❗㧘ߔߥࠊߜ㧘㉄ൻ߇㕖Ᏹߦଦㅴߐࠇߡ㉄ൻ㒐ᱛലᨐࠍ␜ߒ㧘శჇᗵ㉄ൻߦኻߒߡ߽ᛥ೙ലᨐࠍ

޿ࠆߎߣ߇್᣿ߒߚ‫ޕ‬ᓥߞߡ㧘ࠢࡠࡠࡈࠖ࡞ᷝട♽ߢߪ߽ߟߎߣ߇್᣿ߒߚ‫⚿ߩߎޕ‬ᨐ߆ࠄ㧘Ǫࠞࡠ࠹ࡦࠍ

ᷝട㊂ߦ઻޿ࠃࠅᄙ㊂ߩǪࠞࡠ࠹ࡦߩᷝട߇ᔅⷐߢ޽

3600ppm ᷝടߔࠆߣࠢࡠࡠࡈࠖ࡞ߩሽ࿷ߩ᦭ήߦ㑐ࠊࠄࠅ㧘10ppm ᷝടߒߚࠢࡠࡠࡈࠖ࡞ߩ㉄ൻଦㅴലᨐࠍ⋧Ვߕ㉄ൻ෻ᔕࠍ⚂ 1㧛6 ୚ߦᛥ೙ߢ߈ࠆߎߣ߇್᣿ߒ㧘Ǫߔࠆߚ߼ߦߪ㧘Ǫࠞࡠ࠹ࡦࠍ 2000ppm એ਄ᷝടߔࠆߎ

ࠞࡠ࠹ࡦߪࠢࡠࡠࡈࠖ࡞ߦࠃࠅ⊒↢ߒߚ৻㊀㗄㉄⚛ࠍᶖߣ߇ᔅⷐߢ޽ࠆߎߣ್߽᣿ߒߚ‫ޕ‬㊂‫ࡉ࡯࡝ࠝغ‬ᴤ‫ࡉ࡯࡝ࠝغ‬ᴤ‫࡞ࠖࡈࡠࡠࠢع‬ᷝടࠝ࡝࡯ࡉᴤ

(KIࠝ࡝࡯ࡉᴤߣࠢࡠࡠࡈࠖ࡞ᷝടࠝ࡝࡯ࡉᴤߦኻߔࠆǪ́ࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐ㪈㪅㪇㪇㱎㪄䉦䊨䊁䊮㊂㊂ήᷝട㪇㪅㪉㪍‫ࡉ࡯࡝ࠝغ‬ᴤ‫࡞ࠖࡈࡠࡠࠢع‬ᷝടࠝ࡝࡯ࡉᴤ㪍㪇㪇㫇㫇㫄㪇㪅㪋㪌㪈㪉㪇㪇㫇㫇㫄㪈㪏㪇㪇㫇㫇㫄㪇㪅㪍㪈㪇㪅㪏㪊㪉㪋㪇㪇㫇㫇㫄㪈㪅㪈㪏㪈㪅㪉㪋㪊㪇㪇㪇㫇㫇㫄㪊㪍㪇㪇㫇㫇㫄㪈㪅㪌㪏㪇㪇㪅㪌㪈㪈㪅㪌ㆊ㉄ൻ‛ଔ䈏㪈㪇㪇㫄㪼㫈㪆㫂㪾䈮೔㆐䈜䉎䉁䈪䈱ᤨ㑆䈱Ყ㪉(K㨓 ࠝ࡝࡯ࡉᴤߩࠢࡠࡠࡈࠖ࡞ߦࠃࠆ㉄ൻଦㅴ૞↪ߣǪ́ࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐ㧙㧙 Ǫࠞࡠ࠹ࡦߣ࠻ࠦࡈࠚࡠ࡯࡞ߩ౒ሽലᨐ

ᶖ෰೷ߣߒߡߪߎࠇએ਄ߩ㜞޿෻ᔕᕈ߇᳞߼ࠄࠇࠆ‫ޕ‬ (KI ߩ⚿ᨐߢߪࠝ࡝࡯ࡉᴤߦ 170ppm ߩ࠻ࠦࡈࠚࡠ

ࠞࡠ࠹ࡁࠗ࠼ߩ৻㊀㗄㉄⚛ᶖ෰෻ᔕㅦᐲቯᢙߪ

࡯࡞߇฽᦭ߐࠇߡ޿ߚߚ߼㧘Ǫࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐࠍ࠻ࠦࡈࠚࡠ࡯࡞ߣᲧセߔࠆߎߣࠍ⋡⊛ߣߒߡ㧘߹ߕ㧘
Toc ࠍ฽߹ߥ޿♖⵾ࠝ࡝࡯ࡉᴤߦࠢࡠࡠࡈࠖ࡞ 10ppm ࠍᷝടߒߚᓟ㧘Toc ߹ߚߪǪࠞࡠ࠹ࡦࠍᷝടߒߚ⥄േ㉄ൻ⹜㛎ߦߟ޿ߡ  ߣห᭽ߦㆊ㉄ൻ‛ଔ߇ 100meq/kg ߦ೔1010M-1s-1 ⒟ᐲ㧘࠻ࠦࡈࠚࡠ࡯࡞ߩ৻㊀㗄㉄⚛ᶖ෰෻ᔕㅦ

ᐲቯᢙߩ 108M-1s-1 ⒟ᐲߢ޽ࠆߎߣ߇ႎ๔ߐࠇߡ޿ࠆ߇㧠㧕㧘ᧄታ㛎⚿ᨐ߆ࠄ߽ࠝ࡝࡯ࡉᴤߩశჇᗵ㉄ൻߩ㒐ᱛߦߟ޿ߡ㧘Ǫ-ࠞࡠ࠹ࡦߪ㧘৻㊀㗄㉄⚛ߩ‛ℂ⊛ᶖ෰೷ߣߒߡ࠻

ࠦࡈࠚࡠ࡯࡞ߣᲧセߒߡ㜞޿ല₸ࠍ᦭ߔࠆߎߣ߇⏕⹺ߐ㆐ߔࠆᤨ㑆ߢ (KI Ԙ㧘ԙߦ␜ߒߚ‫⚿ߩߎޕ‬ᨐ߆ࠄ㧘Tocࠇߚ‫ޕ‬

ߢ߽ 2 ୚એਅߢ޽ߞߚߩߦኻߒ㧘Ǫࠞࡠ࠹ࡦߢߪ

ࠍᷝടߒߚ♖⵾ࠝ࡝࡯ࡉᴤߦኻߔࠆǪ-ࠞࡠ࠹ࡦߩ㉄ൻ

ࠍ 250-4500ppm ߹ߢᷝടߒߡ߽⺃ዉᦼߩᑧ㐳ലᨐߪᦨᄢ

750-5400ppm ߩᷝടߦࠃߞߡᦨᄢߢ 6.35 ୚߹ߢᑧ㐳ߐࠇ㧘 ᰴߦ㧘ࠢࡠࡠࡈࠖ࡞ 10ppm ߣ࠻ࠦࡈࠚࡠ࡯࡞ 200ppm

㒐ᱛലᨐࠍᬌ⸛ߒ㧘ߘߩ⚿ᨐࠍ (KI Ԛߦ␜ߒߚ‫ߎޕ‬

Toc ߦᲧセߒߡᒝ޿㉄ൻ㒐ᱛലᨐࠍ␜ߔߎߣ߇್᣿ߒߚ‫ޕ‬ߩ⚿ᨐ߆ࠄ࠻ࠦࡈࠚࡠ࡯࡞߇౒ሽߔࠆߣǪࠞࡠ࠹ࡦනߐࠄߦ㧘ਔ⠪ߩᷝട㊂ࠍࡕ࡞ᢙߢᲧセߒߡ߽߿ߪࠅ㧘Ǫ⁛ߩ႐วߦᲧセߒߡ㉄ൻ㒐ᱛലᨐ߇ะ਄ߔࠆߎߣ㧘ߔߥࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐ߇ఝࠇߡ޿ࠆߎߣ߇⏕⹺ߐࠇߚ‫ޕ‬ࠊߜ㧘౒ሽലᨐ߇⏕⹺ߐࠇߚ߇㧘࠻ࠦࡈࠚࡠ࡯࡞ߦࠃࠆ

ߎࠇ߹ߢߦ৻㊀㗄㉄⚛߇⢽⾰ਛߩੑ㊀⚿วߣ෻ᔕߔࠆ㉄ൻ㒐ᱛലᨐߩะ਄ߪ㗼⪺ߢߪߥߊ㧘౒ሽ♽ߦ߅ߌࠆ㉄ᤨߩ෻ᔕㅦᐲቯᢙߪ 10 M s ⒟ᐲߣႎ๔ߐࠇߡ߅ࠅ㧘৻ൻ㒐ᱛലᨐߪǪࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐߦଐሽߒߡ޿㊀㗄㉄⚛ߦࠃࠆ⢽⾰ߩ㉄ൻࠍ㒐ᱛߔࠆߚ߼ߩ৻㊀㗄㉄⚛

ࠆߎߣ߇್᣿ߒߚ‫ ޕ‬

-1 -1

ᷝട㊂

ᷝട㊂

ᷝട㊂

5(K㨓 ࠝ࡝࡯ࡉᴤߦኻߔࠆǪ́ࠞࡠ࠹ࡦߣ࠻ࠦࡈࠚࡠ࡯࡞ߩశჇᗵ㉄ൻ㒐ᱛലᨐ㧙㧙౒ሽ♽ߦ߅ߌࠆǪࠞࡠ࠹ࡦ߅ࠃ߮࠻ࠦࡈࠚࡠ࡯࡞ߩ⋧ ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߩ㉄ൻ㒐ᱛലᨐ੕㑐ଥࠍ⺞ߴࠆߚ߼㧘⹜ᢱᴤߩㆊ㉄ൻ‛ଔ߇ 100meq/kg(KI ߦ ␜ ߒ ߚ ࠃ ߁ ߦ 㧘 ࠕ ࠬ ࠲ ࠠ ࠨ ࡦ ࠴ ࡦ ߪߦ೔㆐ߒߚᤨὐߦ߅ߌࠆǪࠞࡠ࠹ࡦ߅ࠃ߮࠻ࠦࡈࠚࡠ
࡯࡞ߩᱷሽ₸ࠍ᳞߼ߚ‫⚿ߩߘޕ‬ᨐ㧘࠻ࠦࡈࠚࡠ࡯࡞ᱷሽ
₸ߪ޿ߕࠇߩ♽ߢ߽ㆊ㉄ൻ‛ଔߩ਄᣹ߦ઻ߞߡ⋥✢⊛ߦ

800-20,000ppm ߩᷝടߦࠃߞߡ㧘ࠢࡠࡠࡈࠖ࡞ᷝടࠝ࡝

࡯ࡉᴤߦኻߒߡߪㆊ㉄ൻ‛ଔ 100meq/kg ߹ߢߩ೔㆐ᤨ㑆ࠍࠕࠬ࠲ࠠࠨࡦ࠴ࡦήᷝട⹜ᢱߩ 17-19 ୚ߦ߹ߢᑧ㐳ߔૐਅߒߚ߇㧘100meq/kg ೔㆐ᤨߩᱷሽ₸ߪ࠻ࠦࡈࠚࡠ࡯

ࠆߎߣ߇್᣿ߒߚ‫ߒ߆ߒޕ‬㧘ࠢࡠࡠࡈࠖ࡞ήᷝട⹜ᢱߢ

࡞න⁛♽ߢߪ 50㧑⒟ᐲߢ޽ߞߚߩߦኻߒ㧘౒ሽ♽ߢߪߪ 4,000ppm ᷝടߒߡ߽ 4 ୚ߩᑧ㐳ߦߣߤ߹ߞߚߎߣ߆

30㧑ߣૐߊ㧘න⁛♽ߦᲧセߒߡࠃࠅᕆỗߥᷫዋ߇ⷰኤߐ

ࠄ㧘ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߪࠝ࡝࡯ࡉᴤߩ࡜ࠫࠞ࡞ㅪ㎮෻ᔕࠇߚ‫৻ޕ‬ᣇ㧘100meq/kg ೔㆐ᤨߩǪࠞࡠ࠹ࡦߩᱷሽ₸ߪߦࠃࠆ⥄േ㉄ൻߦኻߔࠆ㒐ᱛലᨐߦᲧセߒߡ㧘ࠢࡠࡠࡈǪࠞࡠ࠹ࡦන⁛ᷝട♽ߢߪ⚂ 40㧑ߢ޽ߞߚߩߦኻߒ㧘ࠖ࡞ߦࠃࠆశჇᗵ㉄ൻߦኻߒߡ㕖Ᏹߦ㗼⪺ߥ㒐ᱛലᨐࠍ౒ሽ♽ߢߪ 70㧑⒟ᐲߣ㜞ߊ౒ሽߦࠃࠅǪࠞࡠ࠹ࡦߩᷫዋ߇ᛥ೙ߐࠇߡ޿ࠆߎߣ߇ⷰኤߐࠇߚ‫⚿ߩࠄࠇߎޕ‬ᨐ߆ࠄ㧘Ǫࠞࡠ࠹ࡦߣ࠻ࠦࡈࠚࡠ࡯࡞ߩ౒ሽ♽ߦ߅޿ߡ㧘࠻␜ߔߎߣ߇್᣿ߒߚ‫ߒ߆ߒޕ‬㧘ᧄታ㛎ߢࠕࠬ࠲ࠠࠨࡦ࠴ࡦߣߒߡ↪޿ߚࡇࡘࠕࠬ࠲ࠝࠗ࡞ߪ 8㧑ߩࠕࠬ࠲ࠠࠨࡦ

࠴ࡦએᄖߦ࠻࡝ࠕࠪ࡞ࠣ࡝࠮ࡠ࡯࡞ 36㧑㧘ࠫࠕࠪ࡞ࠣ࡝

ࠦࡈࠚࡠ࡯࡞ߪࠢࡠࡠࡈࠖ࡞ߦࠃࠆࠝ࡝࡯ࡉᴤߩశჇᗵ

࠮ࡠ࡯࡞ 8㧑㧘⢽⢌㉄ 40㧑ߥߤߩ⢽⾰ࠍ฽ߺ㧘ߐࠄߦߎ㉄ൻߦኻߒߡ⋥ធ⊛ߦ㗼⪺ߥ㒐ᱛലᨐࠍ␜ߐߥ޿߇㧘Ǫࠇࠄߩਥ᭴ᚑ⢽⢌㉄ߪࠝ࡟ࠗࡦ㉄㧔C18:1㧕ߣ࡝ࡁ࡯࡞㉄ࠞࡠ࠹ࡦߩᷫዋࠍᛥ೙ߔࠆ૞↪㧘ߔߥࠊߜ㧘Ǫࠞࡠ࠹ࡦ

㧔C18:2㧕ߢ޽ࠆߚ߼㧘⹜ᢱᴤߩ㉄ൻ᜼േߦኻߔࠆߎࠇࠄ

ߦኻߔࠆ଻⼔૞↪ࠍ␜ߒ㧘ߎࠇ߇ਔ⠪ߩ౒ሽ♽ߦ߅ߌࠆߩ౒ሽᚑಽߩᓇ㗀߇ ᔨߐࠇࠆߚ߼㧘੹ᓟ㧘శჇᗵ㉄ൻߦ

㉄ൻ㒐ᱛലᨐߩะ਄ߩⷐ࿃ߢ޽ࠆߣ⠨ኤߐࠇߚ‫ޕ‬ኻߔࠆ㒐ᱛലᨐߩᒝߐߦߟ޿ߡߪࠃࠅ⚐ᐲߩ㜞޿ࠕࠬ࠲䉝䉴䉺䉨䉰䊮䉼䊮ᷝട㊂ࠠࠨࡦ࠴ࡦࠍ↪޿ߡᬌ⸛ߔࠆߎߣ߇ᔅⷐߣ⠨߃ࠄࠇࠆ‫ޕ‬ϭ͘ϬϬϭ͘ϬϬήᷝട‫ࡉ࡯࡝ࠝغ‬ᴤ‫࡞ࠖࡈࡠࡠࠢع‬ᷝടࠝ࡝࡯ࡉᴤϴϬϬƉƉŵϰ͘ϬϰϰϬϬϬƉƉŵϰ͘ϮϯϴϬϬϬƉƉŵϯ͘ϲϵϭϳ͘ϲϬϭϵ͘Ϭϯϭϳ͘ϭϴϮ͘ϱϬϮϬϬϬϬƉƉŵϬϱϭϴ͘ϭϬϭϬϭϱϮϬϮϱㆊ㉄ൻ‛ଔ䈏ϭϬϬŵĞƋͬŬŐ䈮೔㆐䈜䉎䉁䈪䈱ᤨ㑆䈱Ყ(KI ࠝ࡝࡯ࡉᴤߣࠢࡠࡠࡈࠖ࡞ᷝടࠝ࡝࡯ࡉᴤߦኻߔࠆࠕࠬ࠲ࠠࠨࡦ࠴ࡦߩ㉄ൻ㒐ᱛലᨐߘߎߢ㧘ࠝ࡝࡯ࡉᴤߦኻߔࠆࠞࡠ࠹ࡁࠗ࠼ߩ㉄ൻ㒐ᱛ㧠㧚✚ ᜝ ലᨐߦߟ޿ߡᬌ⸛ߒߚ⚿ᨐ㧘Ǫࠞࡠ࠹ࡦᷝട♽ߢߪࠢࡠ ࠝ࡝࡯ࡉᴤߦࠢࡠࡠࡈࠖ࡞ࠍ 050ppm ᷝടߒߚ⥄േ㉄ൻ⹜㛎ߩ⚿ᨐ߆ࠄ㧘ࠝ࡝࡯ࡉᴤߩ㉄ൻ቟ቯᕈߪࠢࡠࡠ
ࡈࠖ࡞ᷝട㊂ߦ઻ߞߡ㗼⪺ߦૐਅߔࠆߎߣ߇್᣿ߒߚ‫ޕ‬Ꮢ⽼ߩࡃ࡯ࠫࡦࠝ࡝࡯ࡉᴤߩࠢࡠࡠࡈࠖ࡞฽᦭㊂ߪ
10ppm ⒟ᐲߢ޽ࠆߎߣ߆ࠄ㧘᦭ലߥ㉄ൻ㒐ᱛᴺߩ㐿⊒߇ᔅⷐߣ⠨߃ࠄࠇߚ‫ޕ‬ࡠࡈࠖ࡞ߩᷝട࡮ήᷝടߦ߆߆ࠊࠄߕࠝ࡝࡯ࡉᴤߩㆊ㉄ൻ‛ଔߩ਄᣹ߪᛥ೙ߐࠇ㧘Ǫࠞࡠ࠹ࡦ 3,600ppm ᷝട⹜ᢱߢߪ 100meq/kg ߦ೔㆐ߔࠆᤨ㑆߇Ǫࠞࡠ࠹ࡦήᷝട

⹜ᢱߦᲧセߒߡ޿ߕࠇߩ♽߽⚂ 6 ୚ߦᑧ㐳ߐࠇࠆߎߣ߇
⏕⹺ߐࠇߚ‫ߚ߹ޕ‬㧘10ppm ߩࠢࡠࡠࡈࠖ࡞ߦࠃࠆ㉄ൻଦㅴ૞↪ߪ㧘Ǫࠞࡠ࠹ࡦࠍ 2000ppm ⒟ᐲᷝടߔࠆߎߣߦ

㧙㧙

67,192-196(1990)ࠃࠅ⋧Ვߢ߈ࠆߎߣ߇್᣿ߒߚ‫৻ޕ‬ᣇ㧘࠻ࠦࡈࠚࡠ࡯࡞
ߪశჇᗵ㉄ൻߦኻߔࠆ㒐ᱛലᨐߪᒙ޿߽ߩߩ㧘Ǫࠞࡠ࠹5㧕 R.Apricio, L.Roda, A. Albi, F. Gutierrez; Effectsof various compounds on virgin olive oil stabilityࡦ౒ሽਅߢߪǪ-ࠞࡠ࠹ࡦߩ㉄ൻ㒐ᱛലᨐࠍჇᒝߔࠆߎ

measured by Rancimat, J.Agric.Food Chem., 47,

ߣ߇⏕⹺ߐࠇߚ‫ߚ߹ޕ‬㧘ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߪ
80020,000ppm ߩᷝടߦࠃߞߡ㧘ࠢࡠࡠࡈࠖ࡞ 10ppm ᷝടࠝ࡝࡯ࡉᴤߦኻߒߡ㧘ㆊ㉄ൻ‛ଔ߇ 100meq/kg ߦ೔㆐ߔࠆᤨ㑆ࠍࠕࠬ࠲ࠠࠨࡦ࠴ࡦήᷝട⹜ᢱߩ 1719 ୚ߦᑧ4150-4155(1999)

6㧕 J. Velasco, C. Dobarganes; Oxidative stability ofvirgin olive oil, Eur. J. Lipid Sci. Technol.,
104,661-676 (2002)㐳ߒߚ߇㧘ࠢࡠࡠࡈࠖ࡞ήᷝട⹜ᢱߢߪࠕࠬ࠲ࠠࠨࡦ࠴
ࡦࠍ 4,000ppm ᷝടߒߡ߽ 4 ୚ߩᑧ㐳ߦߣߤ߹ߞߚߎߣ

7㧕 ᵤᔒ↰⮮ੑ㇢✬㧘㘩ຠߩశഠൻ㒐ᱛᛛⴚ㧘p.29-35㧘
ࠨࠗࠛࡦࠬࡈࠜ࡯࡜ࡓ㧔2001㧕߆ࠄ㧘ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߪశჇᗵ㉄ൻߦኻߒߡࠃࠅ㗼⪺8㧕 ᵤᔒ↰⮮ੑ㇢✬㧘㘩ຠߩశഠൻ㒐ᱛᛛⴚ㧘p.121-130㧘ߥ㒐ᱛലᨐࠍ␜ߔߎߣ߇್᣿ߒߚ‫ޕ‬ࠨࠗࠛࡦࠬࡈࠜ࡯࡜ࡓ㧔2001㧕એ਄ߩ⚿ᨐ߆ࠄ㧘ࡃ࡯ࠫࡦࠝ࡝࡯ࡉᴤߩࠃ߁ߦᧂ♖⵾ߢࠢࡠࡠࡈࠖ࡞ࠍ฽᦭ߔࠆᴤ⢽ߦߟ޿ߡߪࠢࡠࡠࡈࠖ࡞

9㧕 K.Warner, E.N.Frankel; Effects of Ǫ-carotene on
light stability of soybean oil, J.Am.Oil Chem.Soc.,

ߩశჇᗵ૞↪ߦࠃߞߡ㉄ൻ߇ଦㅴߐࠇࠆߚ߼㧘ߘߩ㉄ൻ

64, 213-218 (1987)ߦኻߒߡචಽߥᵈᗧ߇ᔅⷐߢ޽ࠅ㧘Ǫࠞࡠ࠹ࡦ߿ࠕࠬ࠲ࠠࠨࡦ࠴ࡦߥߤߩࠞࡠ࠹ࡁࠗ࠼ࠍ↪޿ߚ㉄ൻ㒐ᱛ╷ࠍ⻠10㧕 ੑᧁ㍈㓶㧘ፉፒᒄᐘ㧘⟤Ớ⌀✬㓸㧘᛫㉄ൻ‛⾰̆ࡈߓࠆߎߣ߇ߘߩ቟ోߥ೑↪ߦኻߒߡ㊀ⷐߢ޽ࠆߎߣ߇್

࡝࡯࡜ࠫࠞ࡞ߣ↢૕㒐ᓮ̆㧘p.59-69㧘ቇળ಴᣿ߒߚ‫ޕ‬

࠲࡯㧔1994㧕
11㧕 ᣣᧄᴤൻቇળ✬㧘ᡷ⸓╙ 2࠮ࡦ ᴤ⢽࡮⢽⾰ߩၮ␆ߣᔕ↪㧘p.147-152㧘ᣣᧄᴤൻቇળ㧔2009㧕ෳ⠨ᢥ₂

1㧕 V. Ruiz-Gutierrez, F. Y. G. Muriana, J. Villar; Virginolive oil and carodiovascular disease, Grasas Aceites, 49,9-29(1998)

2㧕 V.Piironen, G.Lindsay, T. A. Miettinen, J. Toivo,

12㧕 ੑᧁ㍈㓶㧘ፉፒᒄᐘ㧘⟤Ớ⌀✬㓸㧘᛫㉄ൻ‛⾰̆ࡈ࡝࡯࡜ࠫࠞ࡞ߣ↢૕㒐ᓮ̆㧘p.87-97㧘ቇળ಴࠲࡯㧔1994㧕

13㧕 G. N. Jham, F. F. F. Teles, L. G. Campos ; Use of
aqueous HCl/MeOH as esterification reagent foranalysis of fatty acids derived from soybean lipids,A.M.Lampi ; review plant sterols : Biosynthesis,
biological function and their importance to humannutrition, J. Sci. Food Agric.㧘80, 939-966 (1981)

3㧕 F.Visioli, C. Galli; Olive oil phenols and theirpotentional effects on human health, J. Agri FoodChem., 46, 4292-4296 (1998)

࠮ࡦJ. Am. OilChem. Soc., 59, 132-133 (1982)14㧕 ᣣᧄᴤൻቇળ✬㧘ⷙḰᴤ⢽ಽᨆ⹜㛎ᴺ㧘ᥳ 4-2000
㧔2003㧕
15㧕 ᣣᧄᴤൻቇળ✬㧘ⷙḰᴤ⢽ಽᨆ⹜㛎ᴺ㧘ෳ 2.9-1996
㧔2003㧕

4㧕 M.I.Minguez, B. Gandul-Rojas, J. Garrido-Fernandez,L.Gallardo-Guerrero, Pigment present in virgin olive oil,㧙㧙