目第1章

次化学実験とは ·······································································

11.1 化学実験の基本 ···································································

11.2 実験データ ········································································

81.3 化学実験便利帳 ···································································

12第2章

物理化学・分析化学 ·······························································

20

2.1 キレート滴定（水の硬度測定） ··················································

202.2 液体の密度 ········································································

232.3 液体の粘度 ········································································25

2.4 気体の密度 ········································································

272.5 反応速度（一次反応） ····························································302.6 酸・塩基平衡 ······································································

332.7 反応速度（二次反応） ····························································

38

着 ···········································································

41

2.9 電気化学的分析法 ·································································

45

2.8 吸第3章

無機化学 ············································································52

3.1 炭酸カルシウムの合成 ····························································

52

3.2 炭酸カルシウムの粒度分布 ·······················································

58

3.3 炭酸カルシウムの比表面積 ·······················································

62

3.4 フェライトの合成 ·································································

65

3.5 セラミックスの作製と評価 ·······················································

70

3.6 熱重量分析（TGA） ·····························································

75

3.7 熱機械分析（TMA） ·····························································

83

第4章

有機化学・高分子化学 ·····························································

87

4.1 アセトアニリドの合成 ····························································

87

4.2 p ニトロアニリンの合成 ·························································

91

4.3 ベンゾイル乳酸エチルの合成 I··················································

95

4.4 ベンゾイル乳酸エチルの合成 II·················································

98

4.5 ジベンザルアセトンの合成 ·······················································

103

4.6 旋光度測定 ········································································

106

4.7 ポリエステルの合成 ······························································

110

4.8 メタクリル酸メチルの蒸留 ·······················································

114

4.9 ポリメタクリル酸メチルの合成 ··················································

118vi目次

4.10 鎖状高分子モデルの統計実験 ···················································

123

4.11 高分子希薄溶液の粘性 ··························································

1284.12 高分子の温度特性 ·······························································

134

4.13 静的粘弾性 ·······································································

137

4.14 ゴム弾性 ·········································································

141

4.15 結晶性高分子材料の結晶化度 ···················································

144

第5章

149

5.1 糖質・アミノ酸の定性分析 ·······················································

149

5.2 緩衝液の調製と酵素反応 ·························································

153

5.3 ブラッドフォード法を用いたタンパク質定量と分光計の取り扱い ············

157

5.4 微生物培養 ········································································

160

5.5 タンパク質の精製 ·································································

168

5.6 酵素活性測定 ······································································

174

5.7 ゲノム DNA の抽出と定量 ·······················································

186

5.8 遺伝子のクローニング ····························································

189

5.9 プラスミド DNA の抽出と精製 ··················································

194

5.10 RNA の抽出と 1st ストランド cDNA の合成·································

197

5.11 特異的なプライマーによる PCR を用いた遺伝子の増幅 ·····················

200

5.12 プラスミド DNA の制限酵素処理 ··············································

202

5.13 アガロースゲル電気泳動 ························································

204

5.14 DNA フラグメントの抽出と精製（ゲル抽出） ································

206

5.15 ライゲーション ··································································

208

5.16 シークエンス反応 ·······························································

210

5.17 シークエンス反応物の精製 ·····················································

212

5.18 シークエンス解析 ·······························································

214

5.19 塩基配列のデータベース解析 ···················································

216第6章索

生物化学 ············································································

化学工学・機能化学 ·······························································

218

6.1 物質の状態と物性 ·································································

218

6.2 物質収支 ···········································································

228

6.3 物質分離 ···········································································

235

6.4 流体輸送 ···········································································

240

6.5 ミクロサイズ電気分解による水素の発生とその爆発性の評価 ·················

247

6.6 固体高分子形燃料電池による水素 → 電気エネルギー変換 ·····················

251

6.7 色素増感型太陽電池の作成と評価 ···············································

256

引 ······················································································

262