Scientific CMOS
Zyla and Neo sCMOS Cameras
Widen Your Expectations

Introducing the
Zyla 4.2 PLUS
• 82% QEmax• 99.8% linearity• 53 fps (USB 3.0)
• 100 fps (Camera Link)

Scientific CMOS (sCMOS)
technology overview

sCMOS - Imaging without compromise

Scientific CMOS (sCMOS) is a breakthrough technology that offers an advanced set of
performance features that render it ideal to high fidelity, quantitative scientific measurement.
Scientific CMOS can be considered unique in its ability to simultaneously deliver on many key performance parameters,
overcoming the ‘mutual exclusivity’ associated with current scientific imaging technology standards, and eradicating theperformance drawbacks traditionally associated with CMOS imagers.

sCMOS is uniquely capable of simultaneously delivering:• Extremely low noise• Rapid frame rates• Wide dynamic range• High resolution• Large field of view• High Quantum Efficiency (QE)
• Rolling and Global (Snapshot) exposure modes

Neo cameras will literally allow one
to see cells in a new light with ultra
sensitive imaging at speeds neverachieved before - as we have seen in
our tests of vesicle trafficking. Thesescientific CMOS cameras are nota small step, but a quantum leap,that will open up new possibilities ofwhat can be studied in fast cellular
processes, rapid screening, andsuper-resolution imaging.

The multi-megapixel sensors offer a large field of view and high resolution, withoutcompromising read noise, dynamic range or frame rate. Rolling and Global (Snapshot)
shutter readout ensure maximum application flexibility.
Read noise is exceptional, even when compared to the highest performance ‘slow-scan’ CCDs. The fact that an sCMOS device
can achieve 1 electron rms read noise while reading out up to 5.5 megapixels at 100 fps through the 10-tap cameralink interface,or 40 fps through the USB 3.0 interface renders it truly extraordinary in the market. By way of comparison, the lowest noiseInterline CCD, reading out only 1.4 megapixels at ~16 fps would do so with ~10 electrons read noise.
The low noise readout is complemented by up to 33,000:1 dynamic range. Usually, for CCDs or EMCCDs to reach their highest
dynamic range values, there needs to be a significant compromise in readout speed, yet sCMOS can achieve this value while
delivering high frame rates. The unique dual amplifier architecture of sCMOS allows for high dynamic range by offering a large
well depth, despite the relatively small 6.5 µm pixel size, alongside lowest noise. A 1.4 megapixel Interline CCD with similarly small
pixels achieves only ~1,800:1 dynamic range at 16 fps.

ParametersCMOS (Zyla 4.2 PLUS)

Interline CCD

EMCCD

Sensor Format

4.2 megapixel

1.4 to 4 megapixel

0.25 to 1 megapixel

Pixel Size

6.5 µm

6.45 to 7.4 µm

8 to 16 µm

Read Noise

0.9 e- @ 30 fps
1.1 e- @ 100 fps

4 - 10 e-< 1e- (with EM gain)

Full Frame Rate (max.)

Sustained: 100 fps full frame

3 to 16 fps~30 fps

Quantum Efficiency (max.)

82%

60%

90% ‘back-illuminated’

Dynamic Range

33,000:1 (@ 30 fps)

~3,000:1 (@ 11 fps)

8,500:1 (@ 30 fps with low
EM gain)Multiplicative Noise

None

None

1.41x with EM gain

Derek Toomre, PhD., Associate Professor,
Department of Cell Biology, Yale
University School of Medicine, USA

Speed
Resolution

Field of
View

Dynamic
Range

Sensitivity

See page 40 for‘Comparing sCMOS withother detectors’
technical note2

3

Features and Benefits

Neo 5.5

Minimization of dark current to maintain low noise advantage under all exposure conditions.
Minimization of hot pixel blemishes meaning more useful pixels. Fan-off mode for vibration
sensitive set-ups.

TE cooling to -40°C

Rolling and true Global (Snapshot)
shutterMaximum exposure and readout flexibility across all applications. Snapshot for ‘Interline CCD’

1 e read noise

Offers lower detection limit than any CCD.

5.5 megapixel sensor format and6.5 μm pixels

Delivers extremely sharp resolution over a 22 mm diagonal field of view: ideal for cell
microscopy, digital pathology, high content screening and astronomy.

Dark Noise Suppression (DNS)
technology

advantage across range of exposure conditions.

Rapid frame rates>30 fps over extended kinetic series. Burst to memory at 100 fps full frame.

UltraVac™

Sustained vacuum integrity and unequalled cooling with 5 year warranty; complete sensor
protection.

Dual-Gain amplifiers

Maximum well depth and lowest noise simultaneously, affording extended dynamic range of
30,000:1.

GPU Express

Simplify and optimize data transfers from camera to Graphical Processing Unit (GPU) card tofacilitate accelerated GPU processing as part of the acquisition pipeline.

4 GB on-head image buffer

Enables bursts of 100 fps @ full dynamic range. Capture extended kinetic series faster thanPC write speed, avoiding prohibitively expensive PCs.

Sub-microsecond inter-frame gap

Global Shutter offers down to 100 ns inter-frame gap, ideal for PIV applications.

Extensive FPGA on-head dataprocessing

Essential to ensure best image quality and quantitative fidelity from sCMOS technology.

Hardware timestamp

FPGA generated timestamp with 25 ns accuracy.

Dynamic baseline clamp

Essential to ensure quantitative accuracy across the image area and between successive
images of a kinetic series.

Spurious noise filter

Realtime FPGA filter that identifies and compensates for spurious high noise pixels.

Data flow monitor

Innovatively manage acquisition capture rates vs data bandwidth limitations.

Comprehensive trigger modes
and I/O

Communication and synchronization within intricate experimental set-ups.

-

Andor’s Neo sCMOS vacuum cooled camera platform has been engineered from the groundup, specifically to realize the absolute highest sensitivity from this exciting new sensor
technology.
Neo 5.5 offers an exceptionally low dark current and read noise floor detection limit, maintained even under longer acquisition
times, alongside a wide dynamic range of 30,000:1. Speeds of 30 fps (full frame) can be maintained over extended kinetic
series acquisitions, with 100 fps achievable in burst mode.
Neo 5.5 offers an advanced set of unique performance features and innovations, including deep vacuum TE cooling to -40°C,
extensive ‘on-head’ FPGA data processing capability, a 4 GB memory buffer and a Data Flow Monitor. Andor’s UltraVacTM
vacuum process has been implemented to offer not only the necessary deep cooling capability, but also complete protectionof the sensor. These capabilities have been conceptualized to drive the best possible performance, image quality and longevity
from sCMOS technology.

NEW

Neo 5.5 offers both Rolling and true Global (also known as ‘Snapshot’) shutter exposure mechanisms. Snapshot mode
provides an exposure sequence that is analogous to that of an Interline CCD, whereby all pixels begin the exposure
simultaneously and end the exposure simultaneously.
Key Features-40ºC vacuum cooling30 fps / 100 fps burst

Rolling and true Global
shutter modes

30,000:1 dynamic range

Vacuum longevity

Superb image quality

Blemish minimization

Quantitative stability

4 GB on-head memory

Vibration free fan off mode

5.5 megapixel

Fast exposure switching

1 e- noise

GPU Express

exposure capability.

Extremely competitive low dark current of 0.14 e-/pix/sec with fan cooling. Maintains low noise

Neo 5.5 QE curve
Key Specifications
Active Pixels

2560 x 2160

Pixel Size (W x H; μm)

6.5 x 6.5

Sensor size (mm)16.6 x 14

Read Noise (median, e-)Page 4

1.3 @ 560 MHz

Sensor Cooling-40°C

Pixel Well Depth (e-)

30,000Max Readout Rate (MHz)4

1 @ 200 MHz

560 MHz(280 MHz x 2 outputs)

Max Frame Rates (fps)Sustained: 30 fps full frame
Burst: 100 fps full frame

QE max60%

5Features and Benefits

Zyla 5.5
NEW

Andor’s Zyla 5.5 sCMOS camera offers high speed, high sensitivity imaging
in a remarkably light and compact design. Both Rolling and true Global
shutter modes offer extensive application flexibility. Global shutter is ideallysuited to fast multi-dimensional microscopy, offering tight synchronization to‘moving’ peripheral devices such as z-stage or light source.

Highest QE sCMOS sensor, providing optimal signal to noise in low light applications.

Rolling and true Global(Snapshot) shutter

Maximum exposure and readout flexibility across all applications. Snapshot for ‘Interline CCD
mode’ freeze frame capture of fast moving/changing events.

Industry fastest frame rates

100 fps sustained via Camera Link (full frame). Industry fastest USB 3.0 frame rates.

FCS Mode

Offers a market-leading speed of 27,057 fps from a 2560(h) x 8(v) region of interest, ideallymatched to the temporal demands associated with Fluorescence Correlation Spectroscopy.

Low fan vibration

Designed with vibration sensitive experiments in mind, such as super-resolution microscopy.

1.2 e read noise

Offers lower detection limit than any CCD.

5.5 megapixel sensor format and6.5 μm pixels

Delivers extremely sharp resolution over a 22 mm diagonal field of view: Ideal for cell
microscopy, digital pathology, high content screening and astronomy.

Dual-gain amplifiers

Maximum well depth and lowest noise simultaneously, affording extended dynamic range of
25,000:1.

12-bit and 16-bit modes

12-bit for smaller file size and absolute fastest frame rates through USB 3.0; 16-bit for fulldynamic range.

TE cooling to 0°C in 35°C
ambient

Ideal for OEM integration into enclosed systems.

-

Featuring Industry
Fastest USB 3.0
40 fps @ 5.5 megapixel

Zyla is ideally suited to many cutting edge applications that push the boundaries of speed, offering
sustained frame rate performance of up to 100 fps (faster with ROI). A highly cost-effective USB 3.0
version is also available, offering an unparalleled 40 fps (full frame) and 1.2 e- rms read noise, representing
an ideal low light ‘workhorse’ camera solution for both
microscopy and physical science applications, in either research
or OEM environments.
Key Features
Rolling and Global (Snapshot) shutter readout ensures maximum
application flexibility. Global shutter in particular provides an
Compact and light
25,000:1 dynamic range
important ‘freeze frame’ exposure mechanism that emulates that
Rolling and Snapshot
0°C cooling @ up to 35°C
of an Interline CCD, overcoming the transient readout nature of
exposure
ambient
Rolling shutter mode.

‘Conceptualized to dramaticallyoutperform Interline CCD technology
within a ‘mid-range’ price bracket,
Andor’s Zyla 5.5 sCMOS is ideallyplaced to become the new gold
standard workhorse imaging detector.’

Compact and light

100 fps sustained

Superb image quality

1.2 e- noise @ 30 fps

Quantitative stability

5.5 megapixel

GPU Express

Cost effective

Market leading USB 3.0

NEW

Simplify and optimize data transfers from camera to Graphical Processing Unit (GPU) card tofacilitate accelerated GPU processing as part of the acquisition pipeline.

GPU Express
Dark Noise Suppression (DNS)
technology

Extremely competitive low dark current of 0.14 e-/pix/sec with fan cooling. Maintains low noise
advantage across range of exposure conditions.

Sub-microsecond inter-frame
gap

Global Shutter offers down to 100 ns inter-frame gap, ideal for PIV applications.

Water cooled option

Access lowest possible vibration and -10ºC cooling.

High Quantum Efficiency

Optimized for popular green / red emitting fluorophores.

Extensive FPGA on-head dataprocessing

Essential to ensure best image quality and quantitative fidelity from sCMOS technology.

Hardware timestamp

FPGA generated timestamp with 25 ns accuracy.

Dynamic baseline clamp

Essential to ensure quantitative accuracy across the image area and between successive
images of a kinetic series.

Spurious noise filter

Real time FPGA filter that identifies and compensates for spurious high noise pixels.

Zyla 5.5 QE curve
Key Specifications
2560 x 2160

Pixel Size (W x H; μm)

6.5 x 6.5

Sensor size (mm)16.6 x 14
1.2 @ 200 MHz

Zyla sCMOS for OEM

Read Noise (median, e-)

The light and compact form factor
coupled with design and mounting
adaptability, board level or private
labelling options, and unparalleledengineering support, renders the Zyla
highly suited to OEM integration.

Sensor Cooling

0°C (up to +30°C ambient)

Pixel Well Depth (e-)

30,000Please call Andor to discuss how Zyla
can be made to work for you.

6

Active Pixels

Max Readout Rate (MHz)1.45 @ 560 MHz

560 MHz(280 MHz x 2 outputs)

Max Frame Rates (fps)Camera Link: 100
USB 3.0: 40

QE max60%

7Features and Benefits

Zyla 4.2 PLUS

NEW

Highest available photon capture efficiency across visible/NIR, optimized for all common fluorophores.

0.9 e read noise

Lowest read noise sCMOS. Significantly lower than any CCD.

NEW

Market leading USB 3.0 speed

Superb USB 3.0 data transfer efficiency and Zyla’s unique 12-bit high speed mode deliver up
to 53 fps full resolution, 77% faster than competing sCMOS. Follow dynamic processes withimproved temporal resolution.

NEW

FCS Mode

Offers a market-leading speed of 26,041 fps from a 2048(h) x 8(v) region of interest, ideallymatched to the temporal demands associated with Fluorescence Correlation Spectroscopy.

NEW

LightScan PLUS

-

NEW

sCMOS

Zyla 4.2 PLUS is the latest sCMOS technology advancement from Andor.
Offering the highest and broadest sCMOS QE profile available, maximizing at
82%, and ideally suited to a wide range of common fluorophores. The Zyla
4.2 PLUS is also uniquely speed-optimized to deliver a sustained 53 fps at full
resolution through a convenient USB 3.0 interface, 77% faster than competingsCMOS cameras. New on-camera intelligence delivers a significant linearity
improvement, providing unparalleled quantitative measurement accuracy
across the full dynamic range.
The high resolution 4.2 megapixel camera delivers QE
performance that is 10% higher than the previous gold-standard
sCMOS sensor, coupled with < 1 e- read noise and 33,000:1
dynamic range in a light, compact, low vibration design, intended
for both research and OEM usage. The Zyla 4.2 PLUS also
includes application specific capability, such as FCS Mode. FCSMode offers a market-leading speed of 26,041 fps from a2048(h) x 8(v) region of interest, ideally matched to thetemporal demands associated with Fluorescence Correlation
Spectroscopy.

82% QE

Reduce background and improve contrast and resolution in scattering samples. Designed toLight Sheet Microscopy and Line Scanning Confocal Microscopy.

Industry Fastest
USB 3.0 53 fps@ 4.2 megapixel

Key Features

NEW GPU Express facilitates real time data processing.
The Zyla 4.2 PLUS differs fundamentally from Zyla 5.5 in termsof shutter flexibility. Whereas Zyla 5.5 offers both Rolling andtrue Global shutter modes, the Zyla 4.2 PLUS operates in Rolling
shutter mode. However, a mechanism called ‘Simulated Global
Exposure’ is available, whereby a TTL output from the camera canbe used to activate a pulsed light source, emulating the Global
shutter exposure condition, albeit with less efficiency.

allow users to maximise signal and confocality concurrently in applications such as Scanned

Low fan vibration

Designed with vibration sensitive experiments in mind, such as super-resolution microscopy.

4.2 megapixel sensor format
and 6.5 μm pixels

Delivers extremely sharp resolution over a 18.8 mm diagonal field of view; ideal for cell
microscopy and astronomy.

Compact and light

Ideal for integration into space restrictive set-ups. Ideal for OEM.

Rolling shutter and simulated
Global Exposure mode

Rolling shutter mode optimizes read noise and frame rate. Employ simulated Global shutter
method if possibility of Rolling shutter spatial distortion.

Dual-gain amplifiers

Maximum well depth and lowest noise simultaneously, affording extended dynamic range of
33,000:1.

> 80% QE

Cost effectiveNEW

99.8 % linearity

Unparalleled quantitative accuracy of measurement across the full dynamic range.

< 1 e- read noise

33,000:1 dynamic range

NEW

GPU Express

Dark Noise Suppression
Technology

Superb image quality

Simplify and optimize data transfers from camera to Graphical Processing Unit (GPU) card tofacilitate accelerated GPU processing as part of the acquisition pipeline.

TE cooling to 0°C in 27ºC
ambient

Ideal for OEM integration into enclosed systems.

100 fps sustained

Quantitative stability

Market leading USB 3.0
speed

GPU Express

Dark Noise Suppression (DNS)
technology

Extremely competitive low dark current of 0.14 e-/pix/sec with fan cooling. Maintains low noise
advantage across range of exposure conditions.

4.2 megapixel

LightScan PLUS with
CycleMax

Water cooled option

Access lowest possible vibration and -10ºC cooling.

Hardware timestamp

FPGA generated timestamp with 25 ns accuracy.

Dynamic baseline clamp

Essential to ensure quantitative accuracy across the image area and between successive imagesof a kinetic series.

Spurious noise filter

Real time FPGA filter that identifies and compensates for spurious high noise pixels.

iCam

Market leading exposure switching with minimal overheads.

100

Zyla 4.2 PLUS QE curve

90

Key Specifications

80

Featuring LightScan PLUS

Quantum efficiency (%)

70
60

Active Pixels

2048 x 2048

Pixel Size (W x H; μm)

6.5 x 6.5

Sensor size (mm)13.3 x 13.3

50

Read Noise (median, e-)
40
30
20
10
0
300

400

500

600

700

800

900

1000

0.9 @ 216 MHz
1.1 @ 540 MHz

Sensor Cooling

0°C (up to +27°C ambient)

Pixel Well Depth (e-)

30,000Max Readout Rate (MHz)540 MHzMax Frame Rates (fps)Camera Link: 100
USB 3.0: 53

QE max82%

The Andor Zyla provides animpressive combination of fieldof-view, sensitivity, resolution andspeed. It is a highly versatile camerathat can be used for many different
applications.
Kurt Thorn, Assistant Adjunct Professor, UCSF
School of Medicine, California, USA

Wavelength (nm)89Zyla - The Biologist’s Choice
Zyla sCMOS has proven a superb
camera choice for the biologist
and microscopist. Many simply
see the Zyla as an amazing
value, superb price/performance‘workhorse’ camera with which toreplace their existing Interline CCD
and upgrade the performance of
their fluorescence microscope.
Others are driven by distinct
application performance criteriathat only sCMOS can answer.

Quality, Throughput,
Performance, Accessibility…
•H
 igh Sensitivity and Wide Dynamic Range– quantify very weak and very bright structureswith one image.
• Superb Image Quality – high resolution 				 and uniform backgrounds for publication-quality		 imaging.
• Capture Everything – the larger field of view		 matches that of modern microscopes. Achieve
		 better statistics and higher throughput in high		 content experiments.

Zyla - The Physicist’s Choice

Example Areas of Application
Lightsheet Microscopy

Super Resolution Microscopy

LightScan PLUS is a new feature
set available on Zyla 4.2 PLUS has
the following benefits for Light Sheet
Microscopy applications:

The low vibration, high QE (QEmax 82%),
low noise and speed capability of Zyla
4.2 PLUS (USB 3.0 and CameraLink)
is well suited to the particular
detection criteria of single molecule
based ‘STORM / PALM’ approaches,
and is used by some as an alternative
to EMCCDs for this purpose. Note,
this should be considered distinct fromthe general needs of single molecule
microscopy, which are best served
by back-illuminated EMCCD cameras(see Andor iXon EMCCD range).
There is the capability to switch off
interpolative filtering and the provisionof custom blemish maps. GPU Express
for real time data processing.

• Optimize signal to noise AND
confocality concurrently• CycleMax – Maximum frame rates
with reduced dead-time, no needto reset light sheet for each alternate
frame

TIRF Microscopy
The Zyla’s fine pixel resolution, great
sensitivity, large field of view and fast
imaging speed offers a superb choiceof platform for following/tracking fast
processes at the cell membrane.
Multi-wavelength TIRF may benefit
from Zyla 5.5 in Global shutter.

High Content Screening
Zyla sCMOS yields markedly improved
throughput and statistical validity ofdata in high content analysis. For
example, a larger field of view results
in analysis of more cells per image;wider dynamic range means a fieldof variable intensity cells can bequantified in only one acquisition; andhigher sensitivity results in reduced
acquisition times. GPU Express for realtime data processing.

Physiology / Ion Imaging
The fast frame rate and excellent
sensitivity of Zyla is ideally suited tothe particular needs of ion signalling
microscopy. Zyla 4.2 PLUS offers
superlative sensitivity at speed, but
electrophysiology may require the
Global shutter exposure mode of Zyla
5.5 to ensure temporal correlation
across the whole image.

Cell Motility
The motile cell is captured extremely
well by the speed and resolution of
the Zyla. Generally, the Rolling shutterof Zyla 4.2 PLUS is suitable, but caremust be taken of distortive effects if
the cell is moving particularly fast. For
example, it has been noted that the
Zyla 5.5 in Global shutter mode was
required to image motile sperm cells.

• Blazingly Fast – more and more studies of cell		 processes require greater temporal resolution.
		 GPU Express for real time data processing.

Zyla sCMOS has become a wellestablished detector amongst
physicists, biophysicists andastronomers; the advanced
combination of speed, sensitivity anddynamic range enabling new groundto be broken.

Performance and Adaptability• Dual Amplifier – novel pixel architecture means
		 you don’t need to pre-select gain. Access lowest
		 read noise and full well depth simultaneously.
• 1000 fps – access extremely fast frame rates
		 through user definable Region of Interest control,		 suited to many applications within the physical		 sciences. GPU Express for real time data 				 processing.

Particle Imaging Velocimetry (PIV)

The true Global Shutter mode of Zyla
Zyla’s fast frame rate and large field
5.5 facilitates an inter-frame gap ofof view are ideal for this resolution
enhancing technique. GPU Express for down to 100 ns.
real time data processing.

Solar Astronomy
Fast frame rates, wide dynamic range
and great linearity present a very
formidable solution to the specificdetector needs of next generation
large solar telescopes.

Adaptive Optics
Accessing > 1000 fps using ROIs
renders the Zyla an ideal Wavefront
detector. Use with a data splitterto enable direct data access. GPU
Express for real time data processing.

Bose Einstein Condensation

Fluorescence Correlation
Spectroscopy

The FCS mode, now available on Zyla
4.2 PLUS provides market leading
speeds of 26,041 fps from a 2048(h)x8(v) region of interest, ideally matchedto the temporal demands associatedwith Fluorescence Correlation
Spectroscopy.

X-Ray / Neutron Tomography
The Zyla can be readily lens coupled
to scintillators and phosphors,presenting a high resolution, sensitiveand fast solution for tomography.*

The QE profile of Zyla PLUS is very goodin the red/NIR region, ideal for BEC of Rb.

• Low dark current – low read noise is 				 complimented by extremely competitive dark		 current, also ensuring minimized hot pixel 				 blemishes.
• Cooling options – standard camera air cools		 to 0ºC up to +35ºC ambient. Water cooled option
		 available on request.

• Ease of use – designed to get you up and 				 imaging with minimal fuss.
• Flexible – fast or slow, big or small, weak or		 bright … Zyla is adaptable all of your imaging
		 challenges.

• Compact and Light – the extremely small volume
		 footprint of Zyla renders it adaptable to intricate
		 optical set-ups.

10

Lucky / Speckle Imaging• Global shutter – Zyla 5.5 offers this important		 mode that completely avoids spatial distortion,
		 and ensures temporal correlation across all regions
		 of the sensor. Sub-microsecond inter-frame gap,
		 ideal for PIV applications.

• Blemish correction maps and advanced
		 control – upon request, Andor provides bespoke
		 capability to turn off/on blemish correction, for		 those who prefer to perform this themselves.
		 Blemish maps can be provided.

Other biological applications include:
Neuroscience, Vesicle Transport, Parasitology, Blood Flow, Ophthalmology

Example Areas of ApplicationsCMOS image courtesy of Jin Ma, Xinglong Observatory, National Astronomical
Observatory of Chinese Academy of Sciences* Zyla HF
Check out Andor’s new Zyla HF Fiber Optic coupled camera, superb for fastindirect X-Ray applications such as tomography or non-destructive testing.

11

Upgrade Your Microscope’s Performance

Why is Zyla such an impressive all-round imager?

Zyla sCMOS - Disruptive Technology, Familiar Price• Superior performance – vastly superior to Interline across key performance parameters.
• Rolling and Global exposures (Zyla 5.5) – Zyla is unique in offering both these exposure modes in one camera. Global
shutter (Snapshot) is directly analogous to the Interline exposure mechanism.
• Image quality – a huge amount of effort and on-camera (FPGA) intelligence has gone into optimizing image quality in
Zyla.

What was the benchmark imaging detector?
Interline CCD technology has been the dominant ‘workhorse’ detector type for fluorescence cell microscopy for almost 15
years, the benchmark sensor being a 1.4 megapixel, 6.45 µm pixel size device, offering typical read noise floor between 5 and
8 e- rms at modest frame rates of 11-12 fps.

• Flexibility – fast, slow, weak, bright, pixel binned, region of interest, Rolling shutter, Snapshot shutter… Zyla is 		
adaptable to a broad gamut of application requirements.
• Affordable price – with so many superb features, we have endeavoured to make the Zyla accessible to every lab. 		
Request a quote, you’ll be pleasantly surprised!
• USB 3.0 - ‘Plug and play’ interface with industry fastest frame rates.

Upgrade your microscope using theimaging superiority of Zyla sCMOS:

Benefits of Upgrading

4x more pixels

Fundamentally, sCMOS technology has been
conceptualized as a vastly superior alternative toInterline CCDs. Indeed, Andor’s Zyla sCMOS offers
dramatically higher performance, yet remains within thesame price bracket as Interline cameras, and is ideallyplaced to become the new gold standard ‘workhorse’laboratory detector. Importantly, Zyla uniquely comeswith both Rolling and true Global (Snapshot) shutter.

sCMOS

CCD

Key Biological Applications

5x more sensitive

Live cell imaging

10x more dynamic range

Widefield fluorescence microscopy

16x faster

Developmental biology
Embryo studies

5x more sensitive

In particular, Global shutter offers a simple Snapshot
imaging capability, directly analogous to that of Interline
CCDs, offering zero image distortion and perfect forsynchronizing to peripheral devices.

Physiology / Ion Imaging
Neuroscience / Vesicle Transport

5.5 MP sCMOS

Parasitology
Cell Motility

The table below compares some typical performance specifications of a 1.4MP Interline CCD to those of the new Zyla sCMOS,
outlining the approximate factor improvement that is available.
Parameter

Typical Interline CCD
Specifications

Zyla 5.5 sCMOS
Specifications

sCMOS Factor
Improvement (approx.)

Read Noise

6 e-

1.2 e-

5x more sensitive

Sustained Frame Rate

12 fps @ 1.4 MP

100 fps @ 5.5 MP*
200 fps @ 1.4MP ROI*

16x faster

Dynamic Range

2,250:1

25,000:1

Sensor Format

1.4 megapixel

5.5 megapixel

10x more dynamic range
4x more pixels

1.4 MP CCD

4x larger field of view

* Frame rates provided are for the ‘10-tap’ Cameralink model. An even more affordable USB 3.0 model is available, in the caseof the Zyla 5.5 offering 40 fps sustained at full 5.5 megapixel resolution.

1213Performance and Innovations
Extended Dynamic Range
The Andor Neo and Zyla cameras are designed to make use of the innovative dual‘column-level’ amplifier design of the sensors.
Traditionally, sensors require that the user must select up-front between high or low amplifier gain (i.e. sensitivity) settings,
depending on whether they want to optimize for low noise or maximum well depth. The dual amplifier architecture of thesCMOS sensor circumvents this need, in that signal can be sampled simultaneously by both high and low gain amplifiers. As
such, the lowest noise of the chip can be harnessed alongside the maximum well depth, affording widest possible dynamic
range of up to 33,000:1.

Lowest Noise Floor
Andor’s ultra sensitive sCMOS cameras have broken new ground in offering down to 0.9
electron rms read noise, without signal amplification technology.
What is truly extraordinary is that this performance level isachievable at 30 fps, representing 200 MHz pixel readout
speed. Furthermore, even at full readout speed, the read noise
floor is negligibly compromised, maintaining down to 1.3 e­rms at 100 fps. For the best CCD cameras to even approach
2 electrons noise, a readout speed of 1 MHz or slower isrequired. This minimal detection limit renders Andor’s sCMOS
cameras suitable for a wide variety of challenging low light
imaging applications.

Dual Amplifier Architecture:

See page 49 for‘Understanding Read Noise’technical note

Each column within each half of the sensor is equipped with dual column level amplifiers and dual analog-to-digitalconverters (ADC).
This architecture was designed to simultaneously minimize read noise and maximize dynamic range. The dual column
level amplifier/ADC pairs have independent gain settings, and the final image is reconstructed by combining pixel
readings from both the high gain and low gain readout channels to achieve an unprecedented intra-scene dynamic range
from the relatively small 6.5 µm pixel pitch.

Readout Speed (MHz)Neo 5.5 Readout Noise (e-)
Rolling Shutter

Global Shutter

200

1

2.3

560

1.3

2.5

Readout Speed (MHz)Zyla 4.2 PLUS Readout Noise (e-)
Rolling Shutter

200

0.9

540

1.1

(a)
Neo sCMOSHigh contrast image recorded with dual amplifier 16-bit mode of Neo

Interline CCD

Pixels sampled by high gain
amplifier (~800 counts )Pixels sampled by low gain
amplifier (~8,000 counts )Comparative low light images taken with
Neo sCMOS (1.3 electrons read noise @560 MHz) vs. Interline CCD (5 electrons
read noise @ 20 MHz), displayed with same
relative intensity scaling.
(a) LED signal in a light-tight imaging
enclosure, intensity ~30 photons/pixel;(b) Fluorescently labelled fixed cell using a
CSU-X spinning disk confocal microscope(x60 oil objective), each 100 ms exposure,same laser power,

See page 34 for‘Dual Amplifier Dynamic Range’
technical note(b)
Neo sCMOS

Interline CCD
Spurious Noise Filter

Zyla uniquely offers both12-bit and 16-bit modes.
12-bit for smaller file size andabsolute fastest frame rates
through USB 3.0; 16-bit for
full dynamic range.

14

Neo and Zyla platforms both comeequipped with an optional in-built FPGA
filter that operates in realtime to reduce
the frequency of the occurrence ofhigh noise pixels that would otherwise
appear as spurious ‘salt and pepper’noise spikes in the image background.

15Performance and Innovations

Data Flow Monitor

Rapid Frame Rates
The parallel readout nature of sCMOS means it is capable of reaching very rapid frame
rates of up to 100 full frames per second, and much faster with region of interest.
Distinctively, this is accomplished without significantly sacrificing read noise performance, markedly distinguishing thetechnology from CCDs. Andor’s sCMOS cameras are uniquely designed to harness this speed potential.

Array Size

Zyla 5.5 USB 3.0

Zyla 5.5 10-tap

For sustained kinetic series measurements it is possible tobe rate limited by:

Zyla 4.2 PLUS
10-tapZyla 4.2 PLUS
USB 3.0

Rolling shutter

Global shutter

Rolling shutter

Global shutter

Rolling shutter

Rolling shutter

2560 x 2160

40 (30)40 (30)100 (75)

49 (49)--2048 x 2048

53 (40)52 (39)105 (98)

52 (52)101 (101)

53 (40)1920 x 1080

107 (80)

98 (80)200 (200)

97 (97)192 (192)

107 (80)

512 x 512422 (422)

201 (201)422 (422)

201 (201)406 (406)

406 (406)

128 x 128

1691 (1691)

716 (716)

1691 (1691)

716 (716)

1627 (1627)

1627 (1627)

2048 x 8 (FCS mode)

27057 (27057)

4008 (4008)

27057 (27057)

4008 (4008)

26041 (26041)

26041 (26041)

Maximum frame rates achievable from the Zyla 5.5 and Zyla 4.2 PLUS sCMOS USB 3.0 and 10-tap Camera Link versions 12-bit (16-bit)

NEW

Market Leading USB 3.0 Performance

Zyla’s speed optimized USB 3.0 interface delivers an
unparalleled 40 fps from 16-bit mode and 53 fps from Zyla’s
unique 12-bit mode (4.2 megapixel array).

The sCMOS sensor in Neo and Zyla iscapable of extremely fast data read rates,
but this in itself imposes considerable
challenges.

(a) bandwidth of the Camera Link interface connecting the		 camera to the PC

e.g. 1 - Requested kinetic series within capability of Camera
Link data transfer bandwidth and Hard Disk Drive write speed.

(b) hard drive write speed
In such circumstances the true frame rate threshold also
depends on many set-up factors, including exposure time,
ROI size, binning, pixel readout rate and choice of single ordual amplifier data.
The Data Flow Monitor, available through Andor Solisacquisition and analysis software, has been innovated toprovide a simple visual tool that enables you to instantly
ascertain if your acquisition parameters will result in a rate ofdata transfer that is too fast for either interface or hard drive.
It will also determine if the kinetic series size is within thecapacity of camera memory, hard drive space or PC RAM.

e.g. 2 - Hard Disk Drive will not write data fast enough for therequested kinetic series. Advised to first reduce data rate.

iCam fast exposure switching
Neo and Zyla benefit from Andor’s iCam technology, aninnovation that ensures minimal overheads associated with
fast exposure switching.
This is particularly important during multi-color microscopy
acquisition protocols, whereby it is necessary to repeatedly
and rapidly flip between pre-set exposure times matched to
the relative signal intensity of each fluorophore.

Our experiments with Andor’s new
sCMOS camera have been highly
encouraging. The combination ofvery low noise sensitivity at rapid
frame rates, coupled with high pixel
resolution and large dynamic range,
will enable us to investigate single
molecules at timescales which werepreviously not accessible.

iCam offers market leading acquisition efficiency, whether
software or externally triggered.

NEW

GPU Express

The Andor GPU Express library has been created to simplify
and optimize data transfers from camera to a CUDA-enabled
NVidia Graphical Processing Unit (GPU) card to facilitate
accelerated GPU processing as part of the acquisition
pipeline. Integrates easily with Andor SDK3.

Prof Stefan Diez, Heisenberg Professorshipfor BioNanoTools, Max Planck Institute of
Molecular Cell Biology and Genetics,
Dresden, Germany

1617Performance and Innovations

UltraVac™ (Neo only)
The Andor Neo is the only vacuum housed CMOS sensor available on the market, offeringsuperior quality, performance and longevity.

Deep Thermoelectric Cooling
Andor’s Neo offers the deepest sensor cooling available from any CMOS imaging cameraon the market, minimizing both dark current and hot pixel blemishes. Additionally, throughthe use of water cooling, the fan can be switched off in the software to minimize camera
vibration; ideal for set-ups that are particularly vibration sensitive.
Neo Cooling Temperature

Dark current (e /pix/sec)-30ºC (fan cooling)

0.015-40ºC (10ºC liquid)

0.007

-

sCMOS @ -40ºC

sCMOS @ +5ºC

Andor’s proprietary UltraVac™ process has a proven track record of field reliability, accumulated over more than 15 years ofshipping high-end vacuum cameras. Using a proprietary technique, we have adapted these process for use with the additional
connections associated with the sCMOS sensor.

• Permanent hermetic vacuum seal
• Sustained deep TE cooling• No risk of condensation

Deep TE cooling is useful for a number 		
of reasons:
Minimization of dark current

Minimization of vibration

Exposure time: 1 s

NTY
RA

AC

V

Hot pixels are spurious pixels with significantly higher darkcurrent than the average and can be problematic even underrelatively short exposure times. Cooling has a major influencein minimizing the occurrence of such events, offering bothan aesthetically cleaner image and a greater number of
unfiltered, usable pixels.

Thermal noise can sacrifice the sCMOS low detection limit. Low
light images recorded with a Neo sCMOS camera at +5ºC and-40ºC sensor cooling temperatures; 50 sec exposure time; 200 MHz
readout giving 1 electron read noise.

U U M WA

Single input
Window(Anti-reflection
Coated)Heat
Removal

5 Year Vacuum
Warranty

O SCMOSNE
YEAR

Minimization of hot pixel blemishes

Vacuum• No maintenance / re-pumping• Minimize out-gassing

sCMOS cannot be considered a truly flexible, workhorse
camera unless dark current contribution has been minimized.
Deep cooling means the low noise advantage can be
maintained under all exposure conditions.

Permanent,
All-Metal SealR

Our faith in the unique sCMOS
vacuum process used in Neomeans that we are proud to offer
an extensive 5 year warranty on thevacuum enclosure.

sCMOS
SensorTE Cooler

Schematic of the Neo sCMOS permanent vacuum head

+5ºC
-40ºC

Many optical configurations are sensitive to vibrations fromthe camera fan.
Andor’s Neo offers:(a) Two fan speeds(b) The ability to turn off the fan, either temporarily or 				 permanently if flowing liquid through the camera
		 (the latter also allows the Neo to be stabilized at -40ºC)
Hot pixel blemishes are significantly reduced at deeper coolingtemperatures, requiring much reduced pixel correction. Uncorrected
images are shown above for 1 sec exposure.

See page 36 for‘Importance of TE Cooling’technical note

18

Thermostatic Precision
The temperature sensor in the Neo and Zyla sCMOS
cameras measures with a thermostatic precision of 0.05ºC

19

Performance and Innovations

Large Field of View

Advanced FPGA on-head processing
Andor’s Neo and Zyla cameras are each equipped with considerable FPGA processing
power. This is essential in order to dynamically normalize data at the pixel level for minor
variations in bias offset, thus eradicating fixed pattern noise associated with this CMOS
phenomenon. This superior dynamic processing capability is also utilized to optionally filter
the small percentage of spurious noise pixels from the image.

The multi-megapixel sensors present in the Neo
and Zyla offer an extended field of view, markedly
exceeding the FOV available from alternative Interline
CCD devices.
Flexibility is key however, and if a large FOV is not required for a particularapplication, Neo and Zyla offer a range of pre-selected ROI sizes at the clickof a button, as well as user defined (with single pixel granularity).
• 21.8 mm diagonal (Zyla 5.5 and Neo 5.5); 18.8 mm diagonal (Zyla 4.2 PLUS)

Pixel-level bias offset compensation
With pixel compensation

The advanced processing power and memory capacity permits
implementation of bias offset compensation for every pixel in thearray. This ultimately relates to a lower noise background.

• Closely matched to modern microscopes• Pre-selected ROIs to quickly opt for smaller FOV if required• x 3.5 larger than popular 512 x 512 EMCCD sensor• x 3.9 larger than popular 1.4 MP Interline CCD sensor

Dynamic baseline clamp• Combine large FOV with dual wavelength image splitter (OptoSplit)

A real time algorithm that uses dark reference pixels on eachrow to stabilize the baseline (bias) offset. Necessary to ensure
quantitative accuracy across each image and between successive
images.

Without pixel compensation

5.5 megapixel sCMOS

Spurious noise filter
An optional real time filter that identifies and compensates for‘spurious’ high noise pixels that are greater than 5 electrons (< 1%of all pixels).

CMOS data requires compensation for fixed patternvariation. This is accomplished in real time for every
pixel within the FPGA of Andor’s sCMOS cameras,
essentially eliminating this noise source from theimage.

Andor offer the capability to switch off interpolative pixel filteringand provision of custom blemish maps, important for applications
such as super-resolution microscopy or astronomy.
Neo sCMOS

Competitor sCMOS

1.4 megapixelInterline CCD
Dynamic Baseline Clamp affords superior background image quality (Dark
image, Rolling shutter, 10 ms exposure)

6.5 μm pixel size combined with 30,000 electron well depth
The 6.5 μm pixel present in Neo and Zyla has been
specifically designed to offer an optimal balance of opticalresolution, photon collection area and well depth. This pixel
size has been determined to provide ideal over-sampling of
the diffraction limit in typical cell microscopy with x 60 and
x 100 objectives.

20• Ideal balance of resolution, photon collection and well		 depth• Superb 30,000 electron well depth• No pixel binning required = no doubling of read noise

Field of View Comparison
Neo 5.5 and Zyla 5.5 Field of View vspopular 1.4 megapixel Interline CCD• No demagnification optics = no wasteful photon loss

21

Performance and Innovations

Key Benefits of true Global shutter (Zyla 5.5 and Neo 5.5)
Global shutter in particular is viewed as an important mode for the biologist, as its benefits are deeply synergistic withthe core imaging requirements of live cell microscopy.

Rolling and Global (Snapshot) Shutter Modes
Neo 5.5 and Zyla 5.5 are distinct in offering both Rolling shutter and true Global shutter
modes from the same sensor, such that the most appropriate mode can be selected
dependent on application requirements.

• No spatial distortion – avoiding the spatial distortion risk of Rolling exposure• 3D / 4D microscopy - recommended for synching to peripheral switching devices• Higher signal to noise due to reduced dead time offering higher ‘effective’ QE• Simplicity – all the benefits of an ‘Interline exposure mode’

Uniquely benefit from a choice…

• Compatible with continuous or pulsed light sources

The 5.5 megapixel sCMOS sensor that is in Zyla 5.5 and Neo 5.5 sCMOS cameras uniquely offers both Rolling and Global
exposure modes. This provides superior application and synchronization flexibility and the ability, through Global exposure, toclosely emulate the familiar ‘Snapshot’ exposure mechanism of Interline CCDs.

Global Exposure is Distortion Free

Rolling and Global Shutter Mechanisms
Rolling and true Global shutter modes describe two distinct types of exposure and readout sequence.
In Rolling shutter, available in all Andor sCMOS cameras, different lines of the array are exposed at different times as theread out ‘wave’ sweeps through the sensor. 10 ms are required at the start to ‘activate’ the sensor to expose, and then 10
ms are required at the end to readout the sensor. Use when not synchronizing to peripheral devices and only when there isa minimal risk of spatial distortion from slow moving sample.
Global shutter exposure and readout (single scan)

Exposure start

Exposure

Exposure End

Rolling shutter exposure and readout (single scan)

Exposure start

Exposure

Readout

In true Global shutter, offered in both Neo 5.5 and Zyla 5.5 models, each pixel in the sensor begins the exposure
simultaneously and ends the exposure simultaneously. This provides a true ‘Snapshot’ exposure capability for moving
samples that is both ‘photon-efficient’ and easy to synchronize to, especially useful for 3D / 4D microscopy. Zyla 4.2 PLUS,
while utilizing a Rolling shutter sensor, offers a Simulated Global Exposure mechanism to overcome risk of spatial distortion.
This mechanism is more elaborate and less photon/time efficient than true Global shutter. True Global Shutter is alsoessential for applications such as Particle Imaging Velocimetry (PIV), where sub-microsecond inter-frame gaps are required.

Rolling

Correct

Distorted

If light is falling on the sensor during the ‘transient’ phases (first 10
ms and final 10 ms) of the Rolling shutter exposure mechanism, andan object is moving during this time, then there is a chance of somedegree of spatial distortion. The degree of distortion is dictated by the
relative size, direction and speed of the object. Global shutter avoids
spatial distortion since there are no ‘transient’ exposure phases.
These images show the head of a moving sperm cell, imaged by the
Neo sCMOS in both Global and Rolling shutter. Distortion of the shapeof the sperm head is evident in Rolling shutter.

For our work on quantifying red
blood cell velocity in the retinal
capillaries we elected to operate in
Global shutter mode, which produces
minimally distorted images. When
operating in Rolling shutter mode weobserved significant image warping,even for moderate eye movements.

See page 30 for ‘Rolling and Global
shutter’ technical note

The customer is highly advised to make an informed choice before purchasing, since in microscopy the more photon-efficient
Global shutter approach can actually result in a higher signal to noise, faster synchronized frame rates and non-distorted
images.

22

GlobalDr. Phillip Bedggood, Metha Laboratory, Department ofOptometry and Vision Sciences, University of
Melbourne, Australia

What should I be aware of as a buyer? Beware of ‘Gen II’ claims!
This topic carries particular relevance, as not all ‘scientific CMOS’cameras on the market offer a choice of Rolling and true Global
exposure. Most offer one or the other. In fact, a sensor that iscurrently being widely positioned in the market as “Gen II”, isactually the sensor used in the Zyla 4.2 PLUS. However, we would
not go so far as to describe this as a 2nd generation sensor, as itachieves a higher Quantum Efficiency at the notable expense oftrue Global shutter capability.

• Sub-microsecond inter-frame gap, ideal for PIV applications

To find out more about how to synchronize
to Rolling and Global shutter (and to view
our FAQs) visit andor.com/learning andread the ‘Synchronizing to Andor sCMOS
Cameras’ technical note.

23Performance and Innovations

sCMOS Software Solutions

Comprehensive trigger functionality

Andor Solis

Neo and Zyla offer a selection of advanced trigger modes, designed to provide tight synchronization of the camera within avariety of experimental set-ups. Triggering is compatible with both Rolling and Global shutter modes.

Solis is a ready to run Windows package with richfunctionality for data acquisition and image analysis/
processing.

• External TTL, Software and Internal trigger (including Simulated Global Exposure - Zyla 4.2 PLUS)• ‘Time Lapse’ and ‘Continuous’ (overlapped) kinetic series

Andor SDK3 and GPU Express• Fast exposure switching (iCam)

Trigger Mode

Description

Trigger Sources

Time Lapse

Each exposure started by a trigger event (e.g. TTL rising edge). Exposure
duration is internally defined.

Internal, External Software

Continuous

Exposures run back to back with no time delay between them. Exposure
time defined by time between consecutive trigger events.

Internal, External

External Exposure

Exposure time defined by TTL width (sometimes known as ‘bulb mode’).

External

External Start

TTL rising edge triggers start of internally defined kinetic series.

External trigger, followed
by internal timer

Available Neo and Zyla trigger modes, applicable to both Rolling and Global shutter.

Andor’s sCMOS camera is the ideal
solution for imaging flow cytometry
where both high speed and low readnoise are critical.

Andor SDK3 allows full control of sCMOS cameras
from your own application, available as 32 and 64
bit libraries for WIndows (7 and 8) and Linux. GPU
Express integrates with SDK3 (Windows), createdto simplify and optimize data transfers from camera
to NVidia GPU card for real time processing of highbandwidth data sets.

Andor iQ
A comprehensive multi-dimensional imaging software
package. Offers tight synchronization of camera witha comprehensive range of microscopy hardware,
along with comprehensive rendering and analysis
functionality.

Bitplane Imaris
Imaris delivers all the necessary functionality forvisualization, segmentation and interpretation ofmultidimensional datasets, up to 100s of Gigabytes in
size.

Dr. Ethan Schonbrun, Rowland Institute,
Harvard University, USA

Third Party Software Compatibility
The range of third party software drivers for Andor’s sCMOS
camera platforms is expanding steadily. Please enquire forfurther details.

Hyperspectral Imaging of Fluorescent Beads. Image courtesy of Dr. Ethan Schonbrun.

24

25

The Andor Imaging Range
Have you found what you are looking for? As an alternative to the sCMOS cameras, Andor
offers an extensive portfolio of high performance low light imaging camera technologies.

iKon CCD

Clara Interline CCD

Neo sCMOS

Deep cooled, low noise CCD

High-performance Interline CCD

Vacuum cooled, lowest noise sCMOS-100ºC coolingIndustry lowest Interline read noise (2.4 e-)

1 electron read noise @ 30 fps

Back-illuminated > 90% QE-55ºC fan cooled; -40ºC vibration free mode

5.5 megapixel / 6.5 μm

1 megapixel to 4 megapixel

1.4 megapixel-40ºC vacuum cooling

Enhanced NIR versions

USB 2.0 true plug and play

30 fps sustained; 100 fps burst‘PV Inspector’ model(Optimized for EL / PL in-line inspection)
USB 2.0 true plug and play

4 GB on head memory
Zyla sCMOS

Fast, sensitive, compact, light sCMOS
1 electron read noise @ 30 fps
5.5 and 4.2 megapixel sensors / 6.5 μm
0ºC cooling at +30°C ambient
100 fps sustained (10-tap Camera Link)
Cost effective USB 3.0 option
16-bit data range

16-bit data range
Fan off vibration free mode

iXon EMCCDHigh performance EMCCD platform

We tested the Andor sCMOS
camera in conjunction with a popularcooled CCD camera, and comparedwith results from a similar test of acompetitor’s scientific CMOS camera.
Andor’s camera showed lowest darknoise, biggest field of view with very
good sampling resolution (number ofpixels), fastest frame rate, compatible
signal to noise ratio and potentiallylargest dynamic range of detection.
It is the most suitable camera on themarket for our project.

Single photon sensitive and back-illuminatedIndustry fastest frame rates-100ºC cooling
Flexible yet intuitive

Dr. Yan Gu, Confocal Imaging and Analysis
Lab National Institute for Medical Research,
London, UK

Quantify in electrons or photons

26

27

Application & Technical Notes
New technology and innovation heralds a lot of new questions!
The Andor Learning Center is dedicated to providing a greater depth of understandingof the performance and innovations associated with the Andor scientific CMOS camera
platform. Deeper insight is provided into areas such as the unique dual amplifierarchitecture (for extended dynamic range), sCMOS read noise distribution, dark noise
effects, vacuum sensor protection and Rolling vs. Global shutter readout modes.
Visit the Learning Center now to discover more at andor.com/learning.
Key sCMOS Resources include:• Rolling and Global Shutter• Dual Amplifier Dynamic Range• The Importance of TE Cooling to sCMOS Technology• Comparing sCMOS With Other Scientific Detectors• Andor sCMOS PC Recommendations and Data Flow Considerations• Understanding Read Noise in sCMOS• Interpolative Blemish Corrections on sCMOS NEW• PIV Mode for Neo and Zyla

NEW

We developed a quantitative phase scanner, capableof imaging an entire microscope slide in less than 50
minutes, with submicron resolution. Due to the highsensitivity of Andor sCMOS cameras we were able
to reduce our exposure time from 100 ms to 15 ms.
The fast frame rates and large field of view of Zyla 5.5
are instrumental in studying fast dynamics in cells andreducing the number of frames to be acquired.

Prof. Gabriel Popescu, Beckman Institute for Advanced
Science and Technology, University of Illinois, USA28

29

Notes...

30

31

Customer Support
Andor products are regularly used in critical applications and we canprovide a variety of customer support services to maximize the return on
your investment and ensure that your product continues to operate at its
optimum performance.
Andor has customer support teams located across North America, Asia
and Europe, allowing us to provide local technical assistance and advice.
Requests for support can be made at any time by contacting our technicalsupport team at andor.com/support.
Andor offers a variety of support under the following format:• On-site product specialists can assist you with the installation and		 commissioning of your chosen product• Training services can be provided on-site or remotely via the Internet• A testing service to confirm the integrity and optimize the performance
		 of existing equipment in the field is also available on request.
A range of extended warranty packages are available for Andor products
giving you the flexibility to choose one appropriate for your needs. These
warranties allow you to obtain additional levels of service and include bothon-site and remote support options, and may be purchased on a multi-yearbasis allowing users to fix their support costs over the operating life cycleof the products.

Project part financed by the European Regional Development Fund under
the European Sustainable Competitiveness Programme for Northern
Ireland.

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