Test Results

2020000553

Order #:Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
4-4-1, Minamisuita
Suita-shi Osaka 564-0043 Japan

PO#: RADS SS 20 0168

Details
Sample(s) from:

Multiple locations

Collection Date
27-Feb-2020

Arrival Date
28-Feb-2020

Approval Date
07-Mar-2020

Notes
Shikata facility, Mouse, Serology testing for routine HM

Diagnostic Summary
TestColony

Tested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.

Printed on 07-Mar-2020 at 2:16 GMTPage 1 of 4Test Results

Order #:2020000553

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HMSerology

ELISA SENDELISA MHVELISA MPUL
ELISA CPIL
ELISA LCMVELISA ECTROMAG C. kutscheri
MAG S.Typhimurium

Results approved by Kato, Katsuhiko on 07 Mar 2020
1234567891012345678910--IN--------

1112131415161718

1920

11121314151617181920----------2122232421222324----IFA CPIL

ELISA SENDELISA MHVELISA MPUL
ELISA CPIL
ELISA LCMVELISA ECTROMAG C. kutscheri
MAG S.Typhimurium

ELISA SENDELISA MHVELISA MPUL
ELISA CPIL
ELISA LCMVELISA ECTROMAG C. kutscheri
MAG S.Typhimurium

Remarks
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction withtissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by other serologic assays
or by testing of additional samples from the same source; PDG = pending; QNS = Quantity not sufficient.

Printed on 07-Mar-2020 at 2:16 GMT
Page 2 of 4

Test Results

Order #:

2020000553

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM

Sample Information
NumberCodeSpecies

1 1

Mouse

2 2

Mouse

3 3

Mouse

4 4

Mouse

5 5

Mouse

6 6

Mouse

7 7

Mouse

8 8

Mouse

9 9

Mouse

10 10

Mouse

11 11

Mouse

12 12

Mouse

13 13

Mouse

14 14

Mouse

15 15

Mouse

16 16

Mouse

ColonyStrainAge

Room 3, Sample Type: SerumICR
13 weeks
Shikata facility Room 3(5105) Sentinel
ICR
13 weeks
Shikata facility Room 3(5105) Sentinel
Room 2, Sample Type: SerumICR
13 weeks
Shikata facility Room 2(5106) Sentinel
ICR13 weeks
Shikata facility Room 2(5106) Sentinel
Room 1, Sample Type: SerumICR
13 weeks
Shikata facility Room 1(5107) Sentinel
ICR
13 weeks
Shikata facility Room 1(5107) Sentinel
Room 4, Sample Type: SerumICR
13 weeks
Shikata facility Room 4(5115) Sentinel
ICR
13 weeks
Shikata facility Room 4(5115) Sentinel
Room 7, Sample Type: SerumICR
13 weeks
Shikata facility Room 7(4104) Sentinel
ICR
13 weeks
Shikata facility Room 7(4104) Sentinel
Room 6, Sample Type: SerumICR
13 weeks
Shikata facility Room 6(4105) Sentinel
ICR
13 weeks
Shikata facility Room 6(4105) Sentinel
Room 5, Sample Type: SerumICR
13 weeks
Shikata facility Room 5(4106) Sentinel
ICR
13 weeks
Shikata facility Room 5(4106) Sentinel
Room 11, Sample Type: Serum
13 weeks
Shikata facility Room 11 ICR(4116) Sentinel
13 weeks
Shikata facility Room 11 ICR(4116) Sentinel
Room 10, Sample Type: Serum
Printed on 07-Mar-2020 at 2:16 GMT
Page 3 of 4Sex

Locatio Dilution
nFemaleback1

Femalefront

1Femaleback1

Femalefront1

Femaleback

1Femalefront

1

Femaleback

1Femalefront

1Femaleback

1Femalefront

1Femaleback

1Femalefront

1Femaleback

1Femalefront

1Femaleback

1Femalefront

1

Test Results

Order #:2020000553

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM

Sample Information
NumberCodeSpecies

17 17

Mouse

18 18

Mouse19 19Mouse

20 20

Mouse

21 21

Mouse

22 22

Mouse

23 23

Mouse

24 24

Mouse

ColonyStrainAge

Room 10, Sample Type: Serum (continued)
13 weeks
Shikata facility Room 10 ICR(4117) Sentinel
13 weeks
Shikata facility Room 10 ICR(4117) Sentinel
Room 9, Sample Type: SerumICR
13 weeks
Shikata facility Room 9(4118) Sentinel
ICR
13 weeks
Shikata facility Room 9(4118) Sentinel
Room 8, Sample Type: SerumICR
13 weeks
Shikata facility Room 8(4119) Sentinel
ICR
13 weeks
Shikata facility Room 8(4119) Sentinel
3109, Sample Type: SerumICR
13 weeks
Shikata facility 3109
Sentinel
3121, Sample Type: SerumICR
13 weeks
Shikata facility 3121
Sentinel

Printed on 07-Mar-2020 at 2:16 GMT
Page 4 of 4Sex

Locatio Dilution
nFemaleback

1

Femalefront

1Femaleback

1Femalefront

1Femaleback

1Femalefront

1Female-1Female-1Test Results

2020000556

Order #:Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
4-4-1, Minamisuita
Suita-shi Osaka 564-0043 Japan

PO#: RADS SS 20 0168

Details
Sample(s) from:

Multiple locations

Collection Date
27-Feb-2020

Arrival Date
28-Feb-2020

Approval Date
07-Mar-2020

Notes
Shikata facility, Mouse, PCR testing for routine HM

Diagnostic Summary
Test

ColonyHelicobacter hepaticus PCR
Helicobacter hepaticus PCR
Helicobacter hepaticus PCR
Helicobacter hepaticus PCR

Shikata facility Room 1 (5107)
Sentinel Mice
Shikata facility Room 5 (4106)
Sentinel MiceTested++/-?

PDG

2100022000+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.

Printed on 07-Mar-2020 at 2:16 GMT
Page 1 of 5

Test Results

Order #:

2020000556

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Molecular Diagnostics:
Infectious Disease PCR

Helicobacter hepaticus PCR
Syphacia obvelata PCR
Aspiculuris tetraptera PCR

Helicobacter hepaticus PCR
Syphacia obvelata PCR
Aspiculuris tetraptera PCR

Helicobacter hepaticus PCR
Syphacia obvelata PCRAspiculuris tetraptera PCR

Results approved by Kato, Katsuhiko on 07 Mar 2020

1234

567891012345678910-----+-

----

1112

13

14

15

16

17

18

19

20

11

12

13

14

15

16

17

18

19

20

-

-+-+-

------

21

22

23

24

21

22

23

24

-

---

Infectious Disease PCR Profiles: Helicobacter hepaticus PCR; Pinworm Screen PCR
Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive
Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous DNA
or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for the purpose ofdetermining the presence of PCR inhibitors.The RNA spike control is also used to evaluate the reverse-transcription of RNA.
Amplification of spike template indicates that there is no detectable inhibition and the assay is valid.

Printed on 07-Mar-2020 at 2:16 GMT
Page 2 of 5

Test Results

Order #:

2020000556

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCode

StrainAge

Sex

Locatio Addition
nInfoFemaleback

13 weeks

Femalefront

Room 2, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 2(5106) Sentinel

Femaleback

13 weeks

Femalefront

Room 1, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 1(5107) Sentinel

Femaleback

13 weeks

Femalefront

Room 4, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 4(5115) Sentinel

Femaleback

13 weeks

Femalefront

Room 7, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 7(4104) Sentinel

FemalebackFemale

front

Femaleback

FemalefrontSpecies

Colony1 1

Mouse

Room 3, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 3(5105) Sentinel

2 2

Mouse

3 3

Mouse

4 4

Mouse

5 5

Mouse

6 6

Mouse

7 7

Mouse

8 8

Mouse

9 9

Mouse

10 10

Mouse

11 11

Mouse

12 12

Mouse

Shikata facility Room 3(5105) Sentinel

Shikata facility Room 2(5106) Sentinel

Shikata facility Room 1(5107) Sentinel

Shikata facility Room 4(5115) Sentinel

ICRICR

ICR

ICR

ICR
13 weeks
Shikata facility Room 7(4104) Sentinel
Room 6, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 6(4105) Sentinel
Shikata facility Room 6(4105) Sentinel

ICR

13 weeks

Printed on 07-Mar-2020 at 2:16 GMTPage 3 of 5

Pooledup to
2:1
Pooledup to2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to2:1Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
No pool

Pooledup to
2:1
Pooledup to
2:1

Test Results

Order #:2020000556

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCodeStrain

AgeSex

Locatio Addition
nInfoFemaleback

13 weeks

FemalefrontRoom 11, Sample Type: Fecal pellet
13 weeks
Shikata facility Room 11 ICR(4116) Sentinel

Femaleback

13 weeks

Femalefront

Room 10, Sample Type: Fecal pellet
13 weeks
Shikata facility Room 10 ICR(4117) Sentinel

Femaleback

13 weeks

Femalefront

Room 9, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 9(4118) Sentinel

Femaleback

13 weeks

Femalefront

Room 8, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 8(4119) Sentinel

Femaleback

13 weeks

Femalefront

3109, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility 3109
Sentinel

Female-Species

Colony

13 13

Mouse

Room 5, Sample Type: Fecal pellet
ICR
13 weeks
Shikata facility Room 5(4106) Sentinel

14 14

Mouse

15 15

Mouse

16 16

Mouse

17 17

Mouse

18 18

Mouse

19 19

Mouse

20 20

Mouse

21 21

Mouse

22 22

Mouse

23 23

Mouse

Shikata facility Room 5(4106) Sentinel

Shikata facility Room 11(4116) Sentinel

Shikata facility Room 10(4117) Sentinel

Shikata facility Room 9(4118) Sentinel

Shikata facility Room 8(4119) Sentinel

ICRICR

ICR

ICR

ICR

3121, Sample Type: Fecal pellet

Printed on 07-Mar-2020 at 2:16 GMT
Page 4 of 5

Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1
Pooledup to
2:1

Test Results

Order #:2020000556

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCode24 24Strain

AgeSpecies

ColonyMouse3121, Sample Type: Fecal pellet (continued)
ICR
13 weeks
Shikata facility 3121
Sentinel

Printed on 07-Mar-2020 at 2:16 GMT
Page 5 of 5Sex

Locatio Addition
nInfoFemale-Pooledup to2:1

Test Results

Order #:

2020000558

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-kuOkayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method

Oriental Yeast Co., Ltd.
4-4-1, Minamisuita
Suita-shi Osaka 564-0043 Japan

PO#: RADS SS 20 0168

Purchase Order

Details
Sample(s) from:

Multiple locations

Collection Date
27-Feb-2020

Arrival Date
28-Feb-2020

Approval Date
07-Mar-2020

Notes
Shikata facility, Rat, Serology testing for routine HM

Diagnostic Summary
Test

ColonyTested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.
Results approved by Kato, Katsuhiko on 07 Mar 2020

Serology

ELISA SENDELISA SDAVELISA MPUL
ELISA CPIL
ELISA HANTMAG B.bronchiseptica
MAG C. kutscheri
MAG S.Typhimurium
IFA CPIL

12345282930

3132IN-IN--IN--RemarksMFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction withtissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by other serologic assays
or by testing of additional samples from the same source; PDG = pending; QNS = Quantity not sufficient.

Printed on 07-Mar-2020 at 2:15 GMT
Page 1 of 2

Test Results

Order #:

2020000558

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama UniversityAdvanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology testing for routine HM

Sample Information
NumberCodeSpecies

1 28

Rat

2 29

Rat

3 30

Rat

4 31

Rat

5 32

Rat

ColonyStrainSample Type: Serum
Wistar
Shikata facility 3107
Sentinel
Wistar
Shikata facility 3108
Sentinel
Wistar
Shikata facility 3119
Sentinel
Wistar
Shikata facility 3104
Sentinel
Wistar
Shikata facility Wet lab
Sentinel

AgeSexDilution

13 weeks

Female113 weeks

Female113 weeks

Female113 weeks

Female128 weeks

Male1

Printed on 07-Mar-2020 at 2:15 GMT
Page 2 of 2Test Results

Order #:2020000560

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method

Oriental Yeast Co., Ltd.
4-4-1, Minamisuita
Suita-shi Osaka 564-0043 Japan

PO#: RADS SS 20 0168

Purchase OrderDetails
Sample(s) from:

Multiple locations

Collection Date27-Feb-2020Arrival Date
28-Feb-2020

Approval Date
07-Mar-2020

Notes
Shikata facility, Mouse, PCR testing for routine HM

Diagnostic Summary
TestColony

Tested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.
Results approved by Kato, Katsuhiko on 07 Mar 2020

Molecular Diagnostics:
Infectious Disease PCR

Syphacia muris PCR

12345282930

3132-----

Infectious Disease PCR Profile: Pinworm Screen PCR
Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive
Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous DNAor RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for the purpose ofdetermining the presence of PCR inhibitors.The RNA spike control is also used to evaluate the reverse-transcription of RNA.
Amplification of spike template indicates that there is no detectable inhibition and the assay is valid.

Printed on 07-Mar-2020 at 2:15 GMT
Page 1 of 2

Test Results

Order #:

2020000560

Charles River Japan(CR RADS Japan)
955 Kamibayashi, Ishioka-shi
Ibaraki 315-0138 Japan

LTM Customer ID: 42510
Okayama University
Advanced Science Research Center, OU
Shikata Campus
2-5-1, Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 JapanNotes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCodeSpecies

1 28

Rat

2 29

Rat

3 30

Rat

4 31

Rat

5 32

Rat

ColonyStrain

AgeSample Type: Fecal pellet
Wistar
13 weeks
Shikata facility 3107
Sentinel
Wistar
13 weeks
Shikata facility 3108
Sentinel
Wistar
13 weeks
Shikata facility 3119
Sentinel
Wistar
13 weeks
Shikata facility 3104
Sentinel
Wistar28 weeks
Shikata facility Wet lab
Sentinel

Printed on 07-Mar-2020 at 2:15 GMT
Page 2 of 2Sex

Addition
InfoFemale

No pool

FemaleNo pool

FemaleNo poolFemale

No poolMale

No pool