1999 GORDON RESEARCHCONFERENCE ON POLYAMINES<<Poster Presentations>>[Poster Set I]
1. J. MoshierContinuing studies on the role of ODC deregulation on tumorigeneictransformation. New studies utilizing inhibitors of polyamine uptake on
progressively tumorigenic human breast cell lines.
2. M. va der Graaf, R. Shipper, G. O. N. Oosterhof, J. A. Shalken, A. A. J.
Verhofstad, A. Heerschap (University Hospital Nijmegen, Netherland)
H MRS of prostatic tissue focused on the detection of spermine, a possible
biomarker of malignant behaviour in prostate cancer.
3. J. Ekstrom (Cornell University, USA)
Structural studies of S-adenosylmethionine decarboxylase.
4. O. Levillain, J.-J. Diaz, I. Reymond, A. Greco, A. Didier (Lyon RTH
Laennec/CNRS, France)
Fine localization of ornithine decarboxylase in the kidney of control andtestosterone-treated female mouse.
5. K. Kiiransen (University of Kuopio, Finland)
Disruption of the mouse spermine synthase gene.
6. R. Shipper, N. Romain, A. A. Otten, J. Tan, W. P. Lange, A. A. J.
Verhofstad (University Hospital Nijmegen, Netherland; Presbyterian Hospitalof Dallas/University of Texas Southwestern Medical Center at Dallas, USA)
Immunocytochemical detection of ornithine decarboxylase.

7. M. Cayre, C. Strambi, P. Charpin, R. Augier, A. Strambi (CNRS, France)
Influence of polyamines on structuring the brain of an adult insect.
8. B. P. Kirby, S. Ryder, J. Renault, N. Seiler, G. G. Shaw (Trinity
College/Universite de Rennes1/IRCAD, France)N1-dansyl-spermine: a new antagonist of spermine's modulatory action at theNMDA receptor macrocomplex.
9. P. Berntsson (Lund University, Sweden)
Differential translational regulation of ornithine decarboxylase
and S-adenosylmethionine decarboxylase during the cell cycle.
10. C.-Lin, V. Tolia, R. Vijesurier (Wayne State University and Children's
Hospital of Michigan, USA)
Essential role of polyamine biosynthesis in intestinal growth induced by earlyweaning.
11. S. M. Oredsson, P. S. H. Berntsson, K. Alm (Lund University, Sweden)
Half lives of ornithine decarboxylase and S-adenosylmethionine
decarboxylase activities during the cell cycle of Chinese hamster ovary cells.
12. W. O'Brien (Baylor College of Medicine, USA)
Understanding the role of arginase II in physiology and in polyaminesynthesis.
13. M. C. Bastida, F. Tejada, A. Cremades, R. Penafiel (University of Murcia,Spain)
Effects of polyamine deprivation on sex hormones and reproductive functions
in mice.
14. L. J. Shore, M. B. Hodgins, S. K. Gilmour, J. D. Pitts, M. E. Finbow (CRCBeatson Institute for Cancer Research, Scotland)
Keratinocyte gap junctional intercellular communication is regulated byornithine decarboxylase and polyamines.

15. K. Alm, S. Oredsson (Lund University, Sweden)
Spermine deficiency induced DNA strand brakes in Chinese hamster ovarycells.
16. C. Audit (Universite de Bordeaux, France)
Mechanism of thalidomide teratogenicity.
17. R. Autelli, P. Gaddi, E. Dametto, L. Persson, S. Crepaldi, G. Bonelli, F. M.
Baccino (University of Turin/Centro CNR di Immunogenetica ed Oncologia
Sperimentale, Italy; University of Lund, Sweden)
Efect of cysteine replacement on ornithine decarboxylase degradation andactivity.
18. C. S. Coleman, A. E. Pegg (Pennsylvania State University, USA)
Ubiquitin targets spermidine/spermine N1-acetylspermine (SSAT) for
degradation by the proteasome.
19. F. Flamigni, A. Facchini, C. Capanni, C. Stefanelli, B. Tantini, C. M.
Caldarera (Universita di Bologna, Italy)
p44/42 mitogen-activated protein kinase is involved in the expression of theornithine decarboxylase in leukaemia L1210 cells.
20. C. Hegardt, G. Andersson, S. Oredsson (Lund University/Active Biotech
Research, Sweden)
Different roles of spermine in glucocorticoid- and FAS-induced apoptosis.
21. E. A. Hudson, L. Howells, M. S. Squires, M. M. Manson (University ofLeicester, UK)
Mechanisms of action of the chemopreventive agent indole-3-carbinol.
22. D. E. McCloskey, A. E. Pegg (Pennsylvania State University, USA)
Altered SSAT activity as a cause of cellular resistance to polyamine
analogues.
23. S. L. Moore, J. L. A. Mitchell (Northern Illinois University, USA)
Antizyme forms originating from the first start site of the AZ-1 gene.

24. P. Berntsson, K. Alm, D. Kramer, C. Porter, S. Oredsson (Lund University,Sweden; Roswell Park Cancer Center, USA)
Early cell cycle effects in Chinese hamster ovary cells treated with N1,
N11-diethylnorspermine (DENSPM).
25. M. Sanjay (University of Pittsburgh, USA)
Cloning and tissue-specific expression of agmatinase: an alternative pathway
for polyamine synthesis in mammalian cells.
26. L. Shantz (The Milton S. Hershey Medical Center, USA)The mechanism of ODC induction during Ras transformation.
27. K. T. Thompson, H. M. Wallace (University of Aberdeen Medical School,UK)
The role of polyamines in cytotoxicity.
28. C. Toth, P. Coffino (University of California, USA)
Regulated degradation of yeast ornithine decarboxylase.
[Poster Set II]29. M. T. Olmo, H. Hayashi, M. A. Medina, F. Sanchez-Jimenez (Universityof Malaga, Spain)
Spectroscopic and direct mutagenesis approaches to the catalytic mechanismof mammalian histidine decarboxylase.
30. A. Khomutov (Bakh Institute of Biochemistry, Russia)
Novel acid-free deprotection of N-(2-hydroxyarylidene) amines.
31. I. D. Algranati (Fundacion Campomar, Argentina)
Expression of an exogenous ornithine decarboxylase gene in Trypanosoma
cruzi epimastigotes.
32. S. Roberts (Oregon Health Sciences University, USA)
Genetic analysis of polyamine metabolism in Leishmania donovani.

33. S. Biondi, S. Fornale, S. Scaramagli, K. Oksman-Caldentey, N. Bagni, P.
Torrigiani (University of Bologna, Italy; VTT Biotechnology & FoodResearch, Finland)
Jasmonate-stimulated polyamine biosynthesis in cultured plant tissues leads toover-accumulation of hydroxycinnamoyl amides and methylputrescine.
34. M. Franceschetti, C. Hanfrey, S. Scaramagli, A. J. Michael (Institute ofFood Research, UK)
Conservation of the bicistronic message structure and rapid processing
characteristics of the S-adenosylmethionine decarboxylase family between
monocots and dicots.
35. C. Hanfrey, A. J. Michael (Institute of Food Research, UK)
The Arabidopsis S-adenosylmethionine decarboxylase is translationallyregulated by the encoded product of an upstream ORF.
36. F. Hennion J. Martin-Tanguy (Universite de Rennes, France)
Amine distribution and content in organs of the subantarctic apecies Pringlea
actiscorbutica are responsive to external conditions.
37 C. Illingworth, A. J. Michael (Institute of Food Research, UK)
Post-translatonal regulation of polyaine biosynthesis in plants; isolation ofornithine decarboxylase-binding protein from Arabidopsis.
38. H. Maki (Japan Women's University, Japan)
Biosynthesis and function of polyamines in growth of rice cells.
39. M. Mayer, S. Sommer, A. Liggett, J. Hamill, A. J. Michael (Institute ofFood Research, UK)
Early events in the regulation of plant polyamine metabolism by woundingand jasmonate.
40. S. Minocha (University of New Hampshire, USA)
Genetic manipulation of the metabolism of polyamines and its effects onrelated pathways.

41. E. Morlon, A. J. Michael (Institute of Food Research, UK)
Use of a 'smart' genetic screen in yeast to isolate Arabidopsis suppressors of
the growth defect caused by low levels of polyamines.
42. R. Minocha, S. Long (USDA Forest Service, USA)
Polyamines as markers of environmental stress in forest trees.
43. D. Serafini-Fracassini, S. Del Duca, D. Dondini, M. Della Mea(University of Bologna, Italy)
Plant transglutaminases: possible roles in cytoskeleton organization andphotosynthesis.
44. A. Tassoni, R. Napier, N. Bagni, M. Venis (University of Bologna, Italy;
Horticulture Research Internatioinal, UK)
Purification of a specific spermidine-binding protein from maize (zea mays L)
coleoptiles.
45. J. Atkinson (Brock University, Canada)
Synthesis of hydroxycinnamoyl amides of polyamines and assessment of theirneurotoxicity to invertebrates in comparison to polyamine spider toxins.
46. E. Csuhai (Transylvania University, USA)The spermine binding site of NRD convertase.
47. K. Samejima (Josai University, Japan)
Application of ultrafiltration for measurements of macromolecule-bound andliberated polyamines in rat liver homogenates.
48. M. Belting, L. Fransson (Lund University, Sweden)
Binding of polyamines to proteoglycans - implications for growth regulation.
49. M. Matsushima, E. Fujiwara, M. Wada, A. Shirahata (Josai University,Japan)
Detection of biotinylated proteins in HTC cells cultured in the presence ofbiotinylpolyamine.

50. M. Halmekyto S. Vujcic, J. Janne, C. W. Porter (Roswell Park Cancer
Institute, USA; University of Kuopio, Finland)Over-expression of spermidine/spermine N1-acetyltransferase activity in
MCF-7 breast carcinoma cells causes growth inhibition and increases
sensitivity to the polyamine analog N1, N11-diethylnorspermine.
51. K. Kaasinen, L. Alhonen, J. Koistinaho, A. Vitanen (University of Kuopio,Finland)
Injury induced glial fibrillary acidic protein expression is reduced in mice byoverexpressing spermidine/spermine N1-acetyltransferase.
52. C. A. Mackintosh, D. J. Feith, A. E. Pegg (Pennsylvania State University,USA)
Characterization of mice and cells deficient in spermine synthase.
53. M. Pietila, T. Hyvonen, J. Parkkinen, M. Halmekyto, L. Alhonen, J. Janne(University of Kuopio, Finland)Life without fat; transgenic mice which overexpress spermidine/spermine
N1-acetyltransferase (SSAT) show accelerated fatty acid breakdodwn.
54. S. Suppola (University of Kuopio, Finland)
Characterization of transgenic mice overexpressing both ODC and SSAT.
55. M. Kaouass, M. Audette R. Poulin (CHUL research Center, Canada)
Polyamine homeostasis is under the control of calcineurin and Ppz
phosphatases in Sccharomyces cerevisiae.
56. K. Kashiwagi, A. Kuraishi, K. Igarashi (Chiba University, Japan)
Identification of amino acid residues in PotE involved in putrescine uptake
and excretion.
57. J. W. Olson, P. Babal, M. Ruchko, M. N. Gillespie (University of SouthAlabama, USA)
Hypoxia regulation of ornithine decarboxylase (ODC) and polyaminetransport in rat pulmonary artery endothelial cells (PAEC).

58. K. Sakata, K. Kashiwagi, K. Igarashi (Chiba University, Japan)
Regulation of polyamine transport by the antizyme.
59. R. S. Weeks, S. M. Vanderwerf, C. L. Carlsson, M. R. Burns, C. L. O'Day,
F. Cai, H. Webb (Oridigm Corporation, USA)
Novel lysine-spermine conjugate alters in vitro and in vivo tumour cell
grorwth through inhibition of polyamine transport.
60. M. Yoshida, D. Meksuriyen, K. Kashiwagi, G. Kawai, K. Igarashi (ChibaUniversity/Chiba Institute of Technology, Japan)
Polyamine stimulation of the synthesis of oligopeptide-binding protein(OppA): involvement of a structural change of the Shine-Dalgarno sequenceand the initiation codon AUG in OppA mRNA.
61. A. K. Mattoo, T. Cassol, A. Handa, R. Mehta, N. Li, N. Ali (USDA-S
Vergetable Laboratory, USA)
Tomato fruits transformed with the yeast SAMDC gene accumulates
spermidine and polyamines.
62. L. Kovassin, D. Soulet, M. Audette, R. Charest-Gaudreault, R. Poulin(Laval University Medical Research Centre, Canada)
Design and evaluation of a spermidine - bodipy conjugate as a probe of thepolyamine transport system.
63. L. Kovassin, M. J. Bonneau, M. D. Jardins, M. Audette, R.
Charest-Gaudreault, R. Poulin (Laval University Medical Research Centre,Canada)
Non-cytotoxic spermidine and norspermidine diamers act as pure antagonistsof polyamine transport and potentiate polyamine depletion caused byalpha-difluoromethylornithine (DFMO).
64. R. Minocha, S. Long, H. Maki, S. C. Minocha (University of NewHanpshire, USA)
Assays for the activities of polyamine biosynthetic enzymes using intacttissues.

65. P. Torrigiana, S. Scaramagli, M. M. Altamura, S. Biondi (University ofBologna, Italy)
Perturbation of the free to conjugated polyamine ratio alters organogenesis intobacco plants.
66. Y. Hanzawa, T. Takahashi, Y. Komeda (Hokkaido University, Japan)
The first genetic evidence of the involvement of a spermine synthese in plantdevelopment.
67. A. Nakabachi, H. Ishikawa (University of Tokyo, Japan)
Unique profile of polyamine composition in aphid's endosymbiotic system.
68. K. Shiokawa, M. Kai, T. Higo, J. Yokosuka, M. Shibata, Y. Yasuhiko, M.
Nagano, E. Takayama, K. Igarashi, H. Fukamachi (University of Tokyo/ChibaUniversity, Japan)
Overexpression of S-adenosylmethionine decarboxylase (SAMDC) inearly Xenopus embryos switches on apoptotic program shortly after MBT andinhibits transition from the blastula to gastrula stage.