Test Results

2021000535

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 21 0169

4-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival Date

Approval Date

17-Feb-2021

18-Feb-2021

01-Mar-2021

Notes
Shikata facility, Mouse, Serology testing for routine HM

Diagnostic Summary
TestColony

Tested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.

Printed on Mar-01-2021 at 05:46 GMT
Page 1 of 6Test Results

2021000535

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM
Results approved by Kato, Katsuhiko on 01 Mar 2021

Serology
123

4567891012345678910ELISA SEND----------ELISA MHV----------ELISA MPUL----------ELISA CPIL----------ELISA LCMV----------ELISA ECTRO----------MAG C. kutscheri----------MAG S.Typhimurium----------

111213

1415

16

1718

19

20

111213

14

15

16

17

18

19

20

ELISA SEND----------ELISA MHV----------ELISA MPUL----------ELISA CPIL----------ELISA LCMV----------ELISA ECTRO----------MAG C. kutscheri----------MAG S.Typhimurium----------

21222324

21222324

ELISA SEND----ELISA MHV----ELISA MPUL----

ELISA CPIL----

ELISA LCMV----ELISA ECTRO----MAG C. kutscheri----MAG S.Typhimurium----Remarks

Printed on Mar-01-2021 at 05:46 GMTPage 2 of 6

Test Results

Order #:2021000535
Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction
with tissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by otherserologic assays or by testing of additional samples from the same source; PDG = pending; QNS = Quantity notsufficient.

Printed on Mar-01-2021 at 05:46 GMT
Page 3 of 6

Test Results

2021000535

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrain

AgeSexLocatioDilutionn3109, Sample Type: Serum
23 23

Mouse

Shikata facility 3109

ICR

14 weeks

Female-114 weeks

Female-1Sentinel
3121, Sample Type: Serum
24 24

Mouse

Shikata facility 3121

ICRSentinel
Room 1, Sample Type: Serum
5 5

Mouse

Shikata facility Room 1

ICR

14 weeks

Female

back

1

ICR

14 weeks

Femalefront

1(5107) Sentinel
6 6

Mouse

Shikata facility Room 1(5107) Sentinel

Room 10, Sample Type: Serum
17 17

Mouse

Shikata facility Room 10

ICR

14 weeks

Femaleback1

ICR

14 weeks

Femalefront

1(4117) Sentinel
18 18

Mouse

Shikata facility Room 10(4117) Sentinel

Room 11, Sample Type: Serum
15 15

Mouse

Shikata facility Room 11

ICR

14 weeks

Femaleback1

ICR

14 weeks

Femalefront

1(4116) Sentinel
16 16

Mouse

Shikata facility Room 11(4116) Sentinel

Room 2, Sample Type: Serum
3 3

Mouse

Shikata facility Room 2

ICR

14 weeks

Female

back

1

ICR

14 weeks

Femalefront1

Femaleback

1(5106) Sentinel
4 4

Mouse

Shikata facility Room 2(5106) Sentinel

Room 3, Sample Type: Serum
1 1

Mouse

Shikata facility Room 3

ICR

14 weeks(5105) Sentinel

Printed on Mar-01-2021 at 05:46 GMT
Page 4 of 6

Test Results

2021000535

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Locatio

Dilutionn
Room 3, Sample Type: Serum (continued)
2 2

Mouse

Shikata facility Room 3

ICR

14 weeksFemalefront1(5105) Sentinel
Room 4, Sample Type: Serum
7 7

Mouse

Shikata facility Room 4

ICR

14 weeks

Femaleback1

ICR

14 weeks

Femalefront

1(5115) Sentinel
8 8

Mouse

Shikata facility Room 4(5115) Sentinel

Room 5, Sample Type: Serum
13 13

Mouse

Shikata facility Room 5

ICR

14 weeks

Female

back

1

ICR

14 weeks

Femalefront

1(4106) Sentinel
14 14

Mouse

Shikata facility Room 5(4106) Sentinel

Room 6, Sample Type: Serum
11 11

Mouse

Shikata facility Room 6

ICR

14 weeks

Femaleback1

ICR

14 weeks

Femalefront1(4105) Sentinel
12 12Mouse

Shikata facility Room 6(4105) Sentinel

Room 7, Sample Type: Serum9 9

Mouse

Shikata facility Room 7

ICR

14 weeks

Femaleback1

ICR

14 weeks

Femalefront1(4104) Sentinel
10 10

Mouse

Shikata facility Room 7(4104) Sentinel

Room 8, Sample Type: Serum
21 21

Mouse

Shikata facility Room 8

ICR

14 weeks

Femaleback1

ICR

14 weeks

Femalefront

1(4119) Sentinel
22 22

Mouse

Shikata facility Room 8(4119) Sentinel

Room 9, Sample Type: Serum

Printed on Mar-01-2021 at 05:46 GMT
Page 5 of 6Test Results2021000535

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Locatio

Dilutionn
Room 9, Sample Type: Serum (continued)
19 19

Mouse

Shikata facility Room 9

ICR

14 weeks

Femaleback1ICR

14 weeks

Femalefront1(4118) Sentinel
20 20

Mouse

Shikata facility Room 9(4118) Sentinel

Printed on Mar-01-2021 at 05:46 GMT
Page 6 of 6Test Results

2021000536

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method

Oriental Yeast Co., Ltd.
PO#: RADS SS 21 0169

Purchase Order

4-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection DateArrival Date

Approval Date

17-Feb-2021

18-Feb-2021

01-Mar-2021

Notes
Shikata facility, Rat, Serology testing for routine HM

Diagnostic Summary
TestColony

Tested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.
Results approved by Kato, Katsuhiko on 01 Mar 2021

Serology
1234525

26

27

28

29

ELISA SEND-----ELISA SDAV-----ELISA MPUL-----ELISA CPIL-----

ELISA HANT-----MAG B.bronchiseptica-----MAG C. kutscheri-----

MAG S.Typhimurium-----Remarks
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction
with tissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by otherserologic assays or by testing of additional samples from the same source; PDG = pending; QNS = Quantity notsufficient.

Printed on Mar-01-2021 at 05:45 GMT
Page 1 of 2

Test Results

2021000536

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Dilution Locatio
n3107, Sample Type: Serum
1 25

Rat

Shikata facility 3107

Wistar

14 weeks

Female1-

14 weeks

Female1-

Wistar

14 weeks

Female1-

Wistar

14 weeks

Female1monitorSentinel
3108, Sample Type: Serum
2 26

Rat

Shikata facility 3108

WistarSentinel
3119, Sample Type: Serum
3 27

Rat

Shikata facility 3119
Sentinel

4 28

Rat

Shikata facility 3119
Sentinelbox
3104, Sample Type: Serum

5 29

Rat

Shikata facility 3104

Wistar

14 weeksSentinel

Printed on Mar-01-2021 at 05:45 GMT
Page 2 of 2Female

1-Test Results

2021000542

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 21 0169

4-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival DateApproval Date

17-Feb-2021

18-Feb-2021

01-Mar-2021

Notes
Shikata facility, Mouse, PCR testing for routine HM

Diagnostic Summary
TestColony

Aspiculuris tetraptera PCR

Shikata facility Room 5 (4106)

Tested++/-?

PDG

210002100021000

Sentinel Mice
Helicobacter hepaticus PCR
Helicobacter hepaticus PCR
Helicobacter hepaticus PCR
Helicobacter hepaticus PCR

Shikata facility Room 5 (4106)
Sentinel Mice
Shikata facility Room 6 (4105)
Sentinel Mice+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.

Printed on Mar-01-2021 at 05:45 GMT
Page 1 of 5

Test Results

2021000542

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 JapanNotes
Shikata facility, Mouse, PCR testing for routine HM
Results approved by Kato, Katsuhiko on 01 Mar 2021

Molecular Diagnostics:
Infectious Disease PCR
1

23

4

5

6

7

8

910

1

2

3

4

567

8910

Helicobacter hepaticus PCR----------

Aspiculuris tetraptera PCR----------Syphacia obvelata PCR----------

1112

13

14

15

16

17

18

192011121314151617181920

Helicobacter hepaticus PCR

-+-+-

-----

Aspiculuris tetraptera PCR--+-------

Syphacia obvelata PCR----------

21

22232421222324

Helicobacter hepaticus PCR----

Aspiculuris tetraptera PCR----

Syphacia obvelata PCR----

Infectious Disease PCR Profile: Helicobacter hepaticus PCR
Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive

Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous
DNA or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for thepurpose of determining the presence of PCR inhibitors.The RNA spike control is also used to evaluate thereverse-transcription of RNA. Amplification of spike template indicates that there is no detectable inhibition and theassay is valid.

Printed on Mar-01-2021 at 05:45 GMT
Page 2 of 5

Test Results

2021000542

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Locatio

Additionn Info-Pooled3109, Sample Type: Fecal pellet
23 23

Mouse

Shikata facility 3109

ICR

14 weeks

FemaleSentinelup to
2:1
3121, Sample Type: Fecal pellet

24 24

Mouse

Shikata facility 3121

ICR

14 weeks

Female-Sentinel

Pooledup to2:1

Room 1, Sample Type: Fecal pellet
5 5

Mouse

Shikata facility Room 1

ICR

14 weeks

Femaleback(5107) Sentinel

Pooledup to
2:1

6 6

Mouse

Shikata facility Room 1

ICR

14 weeks

Femalefront(5107) Sentinel

Pooledup to
2:1

Room 10, Sample Type: Fecal pellet
17 17

Mouse

Shikata facility Room 10

ICR

14 weeks

Femaleback(4117) Sentinel

Pooledup to
2:1

18 18

Mouse

Shikata facility Room 10

ICR

14 weeks

Femalefront(4117) Sentinel

Pooledup to
2:1

Room 11, Sample Type: Fecal pellet
15 15

Mouse

Shikata facility Room 11

ICR

14 weeksFemaleback(4116) Sentinel

Pooledup to
2:1

16 16

Mouse

Shikata facility Room 11ICR

14 weeks(4116) Sentinel

Femalefront

Pooledup to2:1

Room 2, Sample Type: Fecal pellet

Printed on Mar-01-2021 at 05:45 GMT
Page 3 of 5

Test Results

2021000542

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexLocatio

Additionn InfobackPooled

Room 2, Sample Type: Fecal pellet (continued)
3 3

Mouse

Shikata facility Room 2

ICR

14 weeks

Female(5106) Sentinel

up to
2:1

4 4

Mouse

Shikata facility Room 2

ICR

14 weeks

Femalefront(5106) Sentinel

Pooledup to
2:1

Room 3, Sample Type: Fecal pellet
1 1

Mouse

Shikata facility Room 3

ICR

14 weeks

Femaleback(5105) Sentinel

Pooledup to
2:1

2 2

Mouse

Shikata facility Room 3

ICR

14 weeks

Femalefront(5105) Sentinel

Pooledup to
2:1

Room 4, Sample Type: Fecal pellet
7 7

Mouse

Shikata facility Room 4

ICR

14 weeks

Femaleback(5115) Sentinel

Pooledup to
2:1

8 8

Mouse

Shikata facility Room 4

ICR

14 weeks

Femalefront(5115) Sentinel

Pooledup to
2:1

Room 5, Sample Type: Fecal pellet
13 13

Mouse

Shikata facility Room 5

ICR

14 weeks

Femaleback(4106) Sentinel

Pooledup to
2:1

14 14

Mouse

Shikata facility Room 5

ICR

14 weeks(4106) Sentinel

Femalefront

Pooledup to
3:1

Room 6, Sample Type: Fecal pellet

Printed on Mar-01-2021 at 05:45 GMT
Page 4 of 5

Test Results

2021000542

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Locatio

Additionn Infoback

PooledRoom 6, Sample Type: Fecal pellet (continued)
11 11Mouse

Shikata facility Room 6

ICR

14 weeks

Female(4105) Sentinel

up to
2:1

12 12

Mouse

Shikata facility Room 6

ICR

14 weeks

Femalefront(4105) Sentinel

Pooledup to
2:1

Room 7, Sample Type: Fecal pellet
9 9

Mouse

Shikata facility Room 7

ICR

14 weeks

Femaleback(4104) Sentinel

Pooledup to
2:1

10 10

Mouse

Shikata facility Room 7

ICR

14 weeks

Femalefront(4104) Sentinel

Pooledup to
2:1

Room 8, Sample Type: Fecal pellet
21 21

Mouse

Shikata facility Room 8

ICR

14 weeks

Femaleback(4119) Sentinel

Pooledup to2:122 22

Mouse

Shikata facility Room 8

ICR

14 weeks

Femalefront(4119) Sentinel

Pooledup to2:1

Room 9, Sample Type: Fecal pellet
19 19

Mouse

Shikata facility Room 9

ICR

14 weeks

Femaleback(4118) Sentinel

Pooledup to
2:1

20 20

Mouse

Shikata facility Room 9

ICR

14 weeks(4118) Sentinel

Femalefront

Pooledup to
2:1

Printed on Mar-01-2021 at 05:45 GMT
Page 5 of 5

Test Results

2021000543

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 21 0169

4-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival Date

Approval Date

17-Feb-2021

18-Feb-2021

01-Mar-2021

Notes
Shikata facility, Rat, PCR testing for routine HM

Diagnostic Summary
Test

ColonyTested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference. We havesummarized this important information in infectious agent Technical Sheets, which you can view by visiting
http://www.criver.com/info/disease_sheets.
Results approved by Kato, Katsuhiko on 01 Mar 2021

Molecular Diagnostics:
Infectious Disease PCR

Syphacia muris PCR

1234

525262728

29-----

Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive

Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous
DNA or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for thepurpose of determining the presence of PCR inhibitors.The RNA spike control is also used to evaluate thereverse-transcription of RNA. Amplification of spike template indicates that there is no detectable inhibition and theassay is valid.

Printed on Mar-01-2021 at 05:45 GMT
Page 1 of 2

Test Results

2021000543

Order #:Charles River Japan

LTM Customer ID: 42510(CR RADS Japan)

Okayama University

955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Locatio

Additionn InfoFemale-

No pool

Female-No pool

Female-No pool

Sex3104, Sample Type: Fecal pellet
5 29RatShikata facility 3104

Wistar14 weeks

Sentinel
3107, Sample Type: Fecal pellet
1 25

Rat

Shikata facility 3107

Wistar

14 weeks

Sentinel
3108, Sample Type: Fecal pellet
2 26

Rat

Shikata facility 3108

Wistar

14 weeks

Sentinel
3119, Sample Type: Fecal pellet
3 27

Rat

Shikata facility 3119

Wistar

14 weeks

Female-No pool

Wistar

14 weeks

FemaleMonitorNo pool

Sentinel
4 28

Rat

Shikata facility 3119
Sentinelbox

Printed on Mar-01-2021 at 05:45 GMT
Page 2 of 2