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it's 2.0 Self-assembled protein arrays with high signal-to-noise and specific reactivity on aminosilanized multimode ultrathin freestanding electrode array substrates for electrochemical immunoassays. Fabrication of aversatile reusable ultrathin film electrode array substrate (UTFAS) with high signal-to-noise (S/N) ratio andspecificity for electrochemical immunoassays is successfully realized using self-assembled protein arrays(SAPAs) anchored on a thin silica film. By means of a simple one-step biomimetic synthesis, the thin film isdeposited onto a micro-nano copper mesh, which is a 3D hierarchical structure with high S/N ratio and specificreactivity to capture capture the protein arrays with high porosity and roughness. The charge transfer resistance(Rct) is significantly lowered in the UTFA sensing platform by maintaining the ultralow film thickness of 2.85
μm, and therefore, a high S/N ratio is achieved without artifacts caused by the high signal attenuation of thesurface and redox mediator. Meanwhile, the thin film deposition process conforms to the merits of theelectrochemical immunoassay with high reliability, and any other interference can be effectively eliminated by
the specific reaction of the designed antibody. The electrochemical signal is amplified by polymerizing nalkylthiophene on the electrode, and this leads to improved sensitivity. Finally, the UTFA was utilized to detect
carcinoembryonic antigen (CEA), a kind of tumor biomarker, in human serum samples with sensitivity as low as
1.88 pg mL-1. Therefore, this work is expected to provide an effective way for fabricating reusable
electrochemical immunoassay 3ef4e8ef8d
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Cpac Imaging Pro 5.0 Full Crack BETTER