Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

This database lists the cell lines that are currently known to be cross-contaminated or misidentified.
Cell lines are listed in alphabetical order. Table 1 contains those cell lines where there is no authentic stock known to the list contributors. The "Contaminating Cell Line",
in most cases, will have overgrown the claimed original, or will have replaced it by a technical error, and the original cells will no longer exist. Table 2 contains cell lines
where some stocks have been shown to be misidentified, but where authentic stock is known to exist.
This database is meant as a preliminary guide to avoiding suspect cell lines; check each cell line that you use before you start work, to see if others have shown it to be
problematic. You should also test all cell lines (e.g., by STR profiling for human cell lines) and compare with the reference stock profile before use.
Observations made in these lists are based on published reports and details obtained from contributors, cell bank websites, and Wikipedia. "Reference PubMed ID" refers
to the unique ID number assigned by the PubMed database (http://www.ncbi.nlm.nih.gov/pubmed/).
Attributions in the last two columns indicate where these misidentifications were reported and in no way imply responsibility for the cause by the authors or institutions.
This database of cross-contaminated or misidentified cell lines is curated by the International Cell Line Authentication Committee (ICLAC). Researchers are very welcome
to submit additional information regarding misidentified cell lines not listed here, authentic stocks, or any other relevant information. Confusion may also arise from two
different cell lines having the same name; information on these would also be welcome. Please contact standards@atcc.org and copy to the ICLAC Chair, Amanda CapesDavis (acapdav@gmail.com). More information on ICLAC can be found at:
http://standards.atcc.org/kwspub/home/the_international_cell_line_authentication_committee-iclac_
The database was developed by Amanda Capes-Davis and Ian Freshney, and published in 2010. To cite the database or learn more, please refer to:Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, Macleod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (2010) Check your cultures!
A list of cross-contaminated or misidentified cell lines. Int J Cancer 127(1): 1-8. PMID: 20143388.
The authors take neither credit nor responsibility for any of the primary observations and have merely attempted to review and collate data previously available on othersites.

Table 1. Misidentified cell lines where no authentic stock is known
Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

222

Human

Ovarian carcinoma

PA1

Human

Teratocarcinoma

Korch et al, 2012

22710073

2474/90

Human

Gastric carcinoma

HT-29

Human

Colon carcinoma

MacLeod et al, 1999

10508494

Nelson-Rees et al,
19816451928

2563 (MAC-21)

Human

Lung carcinoma

HeLa

Human

Cervicaladenocarcinoma

2957/90

Human

Gastric carcinoma

HT-29

Human

Colon carcinoma

MacLeod et al, 1999

10508494

3051/80

Human

Gastric carcinoma

HT-29

Human

Colon carcinoma

MacLeod et al, 1999

10508494

41M

Human

Ovarian carcinoma

OAW 28Human

Ovarian carcinoma

Wilson et al, 1996

8795574

A2008

Human

Ovarian carcinoma

ME-180

Human

Cervical carcinoma

Korch et al, 2012

22710073

ACC2

Human

Salivary gland, adenoid
HeLa
cystic carcinoma

Human

Cervicaladenocarcinoma

ACC3

Human

Salivary gland, adenoid
HeLa
cystic carcinoma

Human

Cervicaladenocarcinoma

ACCM

Human

Salivary gland, adenoid
HeLa
cystic carcinoma

Human

Cervicaladenocarcinoma

ACCNS

Human

Salivary gland, adenoid
Unknown
cystic carcinoma

Mouse

ACCS

Human

Salivary gland, adenoid
T-24
cystic carcinoma

ADLC-5M2

Human

Lung carcinoma

HeLa

Phuchareon et al,
2009; Zhao et al,
2011
Phuchareon et al,
2009; Zhao et al,
2011
Phuchareon et al,
2009; Zhao et al,2011

19557180,
21868764

Unknown

Phuchareon et al,200919557180

Human

Bladder carcinoma

Phuchareon et al,200919557180

Human

Cervicaladenocarcinoma

MacLeod et al, 1999

10508494

Greene et al, 1972;
Nelson-Rees et al,
1981
Greene et al, 1972;
Nelson-Rees et al,
1981

19557180,
21868764

19557180,
21868764

Aedes aegypti ,Suitor's clone

Mosquito

Not specified

UnknownMoth, Antheraea
eucalypti

Not specified

Aedes vexans
cultureMosquito

Not specified

UnknownMoth, Antheraea
eucalypti

Not specified

AG-F

Human

Lymphoma, Hodgkin

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

Drexler et al, 2003

12592342

AKI

Human

Melanoma

HeLa

Human

Cervicaladenocarcinoma

Yoshino et al, 2006

16643607

ALVA-31

Human

Prostate carcinoma

PC-3

Human

Prostate carcinoma

ALVA-41

Human

Prostate carcinoma

PC-3

Human

Prostate carcinoma

ALVA-55

Human

Prostate carcinoma

PC-3

Human

Prostate carcinoma

ALVA-101

Human

Prostate carcinoma

PC-3

Human

AMDURII
AO

Human
Human

ARO

Human

ARO81-1

Human

Skin
AmnionThyroid, anaplastic
carcinoma
Thyroid, anaplastic
carcinoma

LLC-PK1

Pig, Sus scrofa

Prostate carcinoma
Kidney, normal renal
cells
Cervicaladenocarcinoma

4402510,64519284402510,6451928

van Bokhoven et al,11304728,
2001; Varella-Garcia
11433521
et al, 2001
van Bokhoven et al,11304728,
2001; Pan et al, 2001;
11135436,
Varella-Garcia et al,11433521
2001
van Bokhoven et al,14518029
2003
van Bokhoven et al,14518029
2003
Milanesi et al, 2003

14505435

Nelson-Rees et al,
19816451928
21868764

HeLa

Human

HT-29

Human

Colon carcinoma

Zhao et al, 2011

HT-29

Human

Colon carcinoma

Schweppe et al, 2008 18713817

Page 1Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

AV3

Human

Amnion

HeLa

Human

AZ521

Human

Gastric carcinoma

HuTu 80

Human

B2-17

Human

Astrocytoma

U-251 MG

Human

BCC1/KMC

Misidentification
Reported By

Reference
PubMed ID

Cervicaladenocarcinoma

Lavappa et al, 1976;
Nelson-Rees et al,
1981

1250349,
6451928

Duodenal carcinoma

JCRB website

No PMID

Glioblastoma

JCRB website

No PMID

MacLeod et al, 1999

10508494

Drexler et al, 2003

12592342
22710073

Cervicaladenocarcinoma
Leukemia, acutelymphoblastic, T cell

Human

Basal cell carcinoma

HeLa

Human

BE-13

Human

Leukemia, acutelymphoblastic, T cell

PEER

Human

BG-1

Human

Ovarian carcinoma

MCF-7

Human

Breast carcinoma

Korch et al, 2012

BHP 10-3

Human

Thyroid, papillarycarcinoma

TPC-1

Human

Thyroid, papillarycarcinoma

Schweppe et al, 2008 18713817

BHP 14-9

Human

Thyroid, papillarycarcinoma

M14

Human

Melanoma

Schweppe et al, 2008 18713817

BHP 17-10

Human

Thyroid, papillarycarcinoma

M14

Human

Melanoma

Schweppe et al, 2008 18713817

BHP 2-7

Human

Thyroid, papillarycarcinoma

TPC-1

Human

Thyroid, papillarycarcinoma

Schweppe et al, 2008; 18713817,
Zhao et al, 2011
21868764

BHP 5-16

Human

Thyroid, papillarycarcinoma

M14

Human

Melanoma

Schweppe et al, 2008; 18713817,
Zhao et al, 2011
21868764

BHP 7-13

Human

Thyroid, papillarycarcinoma

TPC-1

Human

Thyroid, papillarycarcinoma

Schweppe et al, 2008 18713817

BIC-1

Human

Esophageal
adenocarcinoma

SW-480, SW-620

Human

Colon carcinoma

Boonstra et al, 2010

20075370

BLIN-1 (alsosubclone 1E8)

Human

Leukemia, acutelymphoblastic, B cell
precursor

NALM-6

Human

Leukemia, acutelymphoblastic, B cell
precursor

Drexler et al, 2003

12592342

BM-1604

Human

Prostate carcinoma

DU-145

Human

Prostate carcinoma

MacLeod et al, 1999

10508494
557237,6451928

BrCA 5

Human

Breast carcinoma

HeLa

Human

Cervicaladenocarcinoma

Nelson-Rees and
Flandermeyer, 1977;
Nelson-Rees et al,
1981

BT-B

Human

Bladder carcinoma

HeLa

Human

Cervicaladenocarcinoma

DSMZ website

No PMID

C13

Human

Ovarian carcinoma

ME-180

Human

Cervical carcinoma

Korch et al, 2012

22710073

C16
Human(MRC-5 derivative)

Lung cells, fetal

HeLa

Human

Cervicaladenocarcinoma

ECACC website

No PMID

C-433

Human

Giant cell tumor, benign RD-ES

Human

Sarcoma (Ewing's)

DSMZ website

No PMID

CAC2

Human

Salivary gland, adenoid
Unknown
cystic carcinoma

Rat

Unknown

Phuchareon et al,200919557180

CaMa (clone 15)

Human

Breast carcinoma

Syrian hamsterand mouse

Unknown

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Unknown

6451928

CaOV

Human

Ovarian carcinoma

HeLa

Human

Cervicaladenocarcinoma

Caov-2

Human

Ovarian carcinoma

NIH:OVCAR-2

Human

Ovarian carcinoma

Korch et al, 2012

22710073

CaVe

Human

Gastric carcinoma

HeLa

Human

Cervicaladenocarcinoma

Nelson-Rees et al,
19816451928

CCL3

Human

Chordoma

Unknown

Mouse

Unknown

Brüderlein et al, 2010 21253487

CH1, CH1cisR

Human

Ovarian carcinoma

PA1

Human

Teratocarcinoma

Korch et al, 2012

645192822710073

Lavappa et al, 1976;
1250349,
Nelson-Rees et al,
6451928
1981
Stoolmiller et al,
4640072,
1972; Nelson-Rees et6451928al, 1981

Chang liver

Human

Liver, normal hepatic
cells

HeLa

Human

Cervicaladenocarcinoma

CHB

Human

Astrocytoma

UnknownRat

Not specified

CHP-100

Human

Neuroblastoma

IMR-32

Human

Neuroblastoma

ECACC website

No PMID

CHP-234

Human

Neuroblastoma

Unknown

Human

Unknown

ATCC website

No PMID

Clom 15

Human

Glioblastoma

UnknownRat

Unknown

Higgins et al, 2010

20951163

Clone 1-5c-4

HumanConjunctiva

HeLa

Human

Cervicaladenocarcinoma

ECACC website

No PMID

Clone-16

Human

Pancreas, fetal
endocrine cells

Unknown

Syrian hamster

Unknown

Matsuba et al, 1988

2903855

Page 2

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

Clone 1A

Rainbow trout,
Oncorhynchus
mykissBlood

CHSE-214

Chinook salmon,
Oncorhynchus
Embryotschawytscha

Perry et al, 2001

19002917

CMP

Human

Rectal adenocarcinoma HeLa

Human

CMPII C2

Human

Rectal adenocarcinoma HeLa

Human

CNDT2

Human

Carcinoid tumour

Unknown

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Human

Unknown

Ellis et al, 2010

20959409

Drexler et al, 2003

12592342

6451928
6451928

CO (= COLE)

Human

Lymphoma, Hodgkin

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

COLO-587

Human

Pancreatic carcinoma

COLO-320DM

Human

Colon carcinoma

ATCC FAQ #11058

No PMID

COLO-677

Human

Lung carcinoma, smallcell

RPMI-8226

Human

Myeloma

DSMZ website

No PMID

COLO-818

Human

Melanoma

COLO-800

Human

Melanoma

MacLeod et al, 1999

10508494

CoLo-TC

Human

Colon carcinoma

COLO-205

Human

Colon carcinoma

RIKEN website

No PMID

Greene et al, 1972;
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Culisita inornata
cultureMosquito

Not specified

UnknownMoth, Antheraea
eucalypti

D18T

Human

Synovial cell

HeLa

Human

D98/AH

Human

Not specified

HeLa

Human

D98/AH2 Clone B

Human

Not specified

HeLa

Human

DAMI

Human

Leukemia, acute
myeloid, M7

HEL

Human

DAPT

Human

Astrocytoma, piloid

HeLa

Human

DD

Human

Malignant histiocytosis

K-562

Human

Det30A

Human

Breast carcinoma,
ascitic fluid

HeLa

Human

Detroit-6 (Det6)

Human

Sternal marrow cells

HeLa

Human

Cervicaladenocarcinoma

Detroit-98

Human

Sternal marrow cells

HeLa

Human

Cervicaladenocarcinoma

Detroit 98/AG

Human

Sternal marrow cells

HeLa

Human

Cervicaladenocarcinoma

Detroit 98/AH-2

Human

Sternal marrow cells

HeLa

Human

Cervicaladenocarcinoma

Detroit 98/AH-R

Human

Sternal marrow cells

HeLa

Human

Cervicaladenocarcinoma

Detroit 98s

Human

Sternal marrow cells

HeLa

Human

Cervicaladenocarcinoma

DJM-1

Human

Squamous cell
carcinoma

BSCC-93

Human

Skin, squamous cell
carcinoma

Yoshino et al, 2006

16643607

DM12
Human
(Tu-167 derivative)

Oral squamous cell
carcinoma

UM-SCC-1

Human

Oral squamous cell
carcinoma (recurrence)

Zhao et al, 2011

21868764

DM14
Human
(Tu-167 derivative)

Oral squamous cell
carcinoma

UM-SCC-1

Human

Oral squamous cell
carcinoma (recurrence)

Zhao et al, 2011

21868764

A-375

Human

Melanoma

Zhao et al, 2011

21868764

A-375

Human

Melanoma

Schweppe et al, 2008 18713817

Thyroid, anaplastic
carcinoma
Thyroid, anaplastic
carcinoma

Not specified
Cervicaladenocarcinoma
Cervicaladenocarcinoma

4402510,6451928
6451928

Honma et al, 1992

1730567

Cervicaladenocarcinoma

ECACC website

No PMID

Leukemia, acute
myeloid, M6

MacLeod et al, 1997; 9447816,
Drexler et al, 2003
12592342

Cervicaladenocarcinoma
Leukemia, chronic
myeloid, blast crisis
Cervicaladenocarcinoma

Nelson-Rees et al,
19816451928

Drexler et al, 2003

12592342

Nelson-Rees et al,1981
Lavappa et al, 1976;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,
19816451928
1250349,
6451928566722,6451928566722,6451928566722,6451928566722,6451928566722,6451928

DRO

Human

DRO90-1

Human

DuPro-1

Human

Prostate carcinoma

PC-3

Human

Prostate carcinoma

EB33

Human

Prostate carcinoma

HeLa

Human

Cervicaladenocarcinoma

ECC-1

Human

Endometrial carcinoma

Ishikawa

Human

Endometrial carcinoma

Korch et al, 2012

22710073

Kidney, normal renalcells

Milanesi et al, 2003

14505435

van Bokhoven et al,
2003
Nelson-Rees, 1979;
Nelson-Rees et al,
198114518029
535908,6451928

ECTC

Cow

Thyroid, embryonic

VeroMonkey, African
green(Cercopithecus
aethiops )ECV-304

Human

Endothelium, normal
cells

T-24

Human

Bladder carcinoma

Dirks et al, 1999;
MacLeod et al, 1999

10614862,
10508494

ED27

Human

Chorionic villus

HeLa

Human

Cervicaladenocarcinoma

Kniss et al, 2002

11869090

Page 3

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

EEK

Horse

Kidney, embryonic renal
NSK
cells

Pig, Sus scrofa

Kidney, normal renalcells

Milanesi et al, 2003

14505435

EH

Human

Leukemia, hairy cell

HK

Human

Leukemia, hairy cell

Drexler et al, 2003

12592342

EJ-1

Human

Bladder carcinoma

T-24

Human

Bladder carcinoma

Masters et al, 2001

11416159

Ej138

Human

Bladder carcinoma

T-24

Human

Bladder carcinoma

Azari et al, 2007

17254797

Cervicaladenocarcinoma

Nelson-Rees et al,
19816451928

ATCC website

No PMID

MacLeod et al, 1999

10508494

MacLeod et al, 1999

10508494

Nelson-Rees et al,
19816451928

Yoshino et al, 2006

16643607

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Nelson-Rees et al,
19816451928

ElCo

Human

Breast carcinoma

HeLa

Human

EPC

Carp, Cyprinus
carpioEpithelial papilloma

Unknown

Fathead minnow,
Pimephales
Unknown
promelasEPLC3-2M1

HumanLung carcinoma

HeLa

Human

EPLC-65

Human

Lung carcinoma

HeLa

Human

ESP1

Human

ETK-1

Human

EU-1

Human

EU-7

Human

Lymphoma, Burkitt

HeLa

Human

Cholangiocarcinoma

SSP-25

Human

Leukemia, acutelymphoblastic, B cell
precursor
Leukemia, acutelymphoblastic, T cell

REH

Human

CCRF-CEM

Human

Human

Subcutis, fetal

EVLC2

Human

Umbilical vein
Unknown
endothelium, transfected

Human

Non-endothelial?

Unger et al, 2002

12453433

F2-4E5

Human

Thymic epithelium

SK-HEP-1

Human

Liver carcinoma

MacLeod et al, 1999

10508494

F2-5B6

Human

Thymic epithelium

SK-HEP-1

Human

Liver carcinoma

MacLeod et al, 1999

10508494

Cervicaladenocarcinoma
Thyroid, papillarycarcinoma
Sarcoma(hemangiosarcoma)

Nelson-Rees et al,
19816451928

Ribeiro et al, 2008

19087340

Human

Not specified

FB2

Human

Fitz-HSA

Dog, Canisfamiliaris

Thyroid, papillarycarcinoma
Sarcoma(hemangiosarcoma)

FL

Human

AmnionFlow 13000

Human

Flow 5000

Human

Flow 6000

Human

Flow 7000

Human

Lung cells, embryonic
fibroblast
Lung cells, embryonic
fibroblast
Lung cells, embryonic
fibroblast
Lung cells, embryonic
fibroblast

Human

Cholangiocarcinoma
Leukemia, acutelymphoblastic, B cell
precursor
Leukemia, acutelymphoblastic, T cell
Cervicaladenocarcinoma

EUE

F255A4

HeLa

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

HeLa

Human

TPC-1

Human

DEN-HSA

Dog, Canisfamiliaris

HeLa

Human

MRC-5

Human

Flow 1000

Human

Flow 1000

Human

Flow 3000

Human

Cervicaladenocarcinoma
Lung cells, embryonic
fibroblast
Lung cells, embryonic
fibroblast
Lung cells, embryonic
fibroblast
Lung cells, embryonic
fibroblast

O'Donoghue et al,
2011
Nelson-Rees et al,
1981; Ogura et al,
1993

21908323
6451928,
8397027JCRB website

No PMID

JCRB website

No PMID

JCRB website

No PMID

JCRB website

No PMID

Nelson-Rees et al,
1981; Drexler et al,
20036451928,
12592342

Nelson-Rees et al,
19816451928

FQ

Human

Lymphoma, Hodgkin

OMK-210

Monkey, Owl
Kidney, normal renal(Aotus trivirgatus ) cells

G-11(HBT-3 derivative)

Human

Breast carcinoma

HeLa

Human

Cervicaladenocarcinoma

GHE

Human

Astrocytoma

T-24

Human

Bladder carcinoma

MacLeod et al, 1999

10508494

Lavappa, 1978;
Nelson-Rees et al,
1981

566722,6451928

Girardi heart

Human

Heart, normal cells

HeLa

Human

Cervicaladenocarcinoma

GM1312Human

MyelomaCorrect name,
incorrect cell type

Human

EBV+ B-lymphoblastoid
Drexler et al, 2003
cell line

12592342

GOS-3

Human

Glioma, mixed astrooligodendroglioma

U-343 MG

Human

Glioblastoma

No PMID

GPS-M

Guinea pig

GPS-PDDSMZ website
Nelson-Rees et al,
1981
Nelson-Rees et al,
19816451928

Spleen, adult cells

Strain L-M

Mouse

Connective tissue

Guinea pig

Spleen, adult cells

Strain L-M

Mouse

Connective tissue

GREF-X

Human

Liver, hepatic
myofibroblast

UnknownRat

Unknown

MacLeod et al, 1999

10508494

GT3TKB

Human

Gastric carcinoma

RERF-LC-A1

Human

Lung carcinoma

Yoshino et al, 2006

16643607

Lung carcinoma, small
cell

Personal
communication, M.
Liscovitch to R.
Nardone

No PMID

Williams, 1980

6244232

van Pelt et al, 2003

12619888

van Pelt et al, 2003

12619888

H249

Human

Lung carcinoma, small
cell

H69

Human

H-494

Human

Prostate carcinoma

HeLa

Human

HepG2

Human

HepG2

Human

H7D7A andderivatives
H7D7B andderivatives

Human
Human

Liver, normal cells(SV40-transformed)
Liver, normal cells(SV40-transformed)

Cervicaladenocarcinoma
Liver, hepatocellular
carcinoma
Liver, hepatocellular
carcinoma

6451928

Page 4

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line
H7D7C andderivatives
H7D7D andderivatives
HAG

ClaimedSpecies
Human
Human
Human

Claimed Cell TypeLiver, normal cells(SV40-transformed)
Liver, normal cells(SV40-transformed)
Thyroid adenoma(goitre)Contaminating
Cell Line

Actual Species Actual Cell Type

HepG2

Human

HepG2

Human

T-24

Human

HBC

Human

Breast carcinoma,
invasive ductal tumor

UnknownRat

HBL-100

Human

Breast carcinoma

Unknown

Human

HBT-3

Human

Breast carcinoma

HeLa

Human

Liver, hepatocellular
carcinoma
Liver, hepatocellular
carcinoma
Bladder carcinoma

Misidentification
Reported By

Reference
PubMed ID

van Pelt et al, 2003

12619888

van Pelt et al, 2003

12619888

MacLeod et al, 1999

10508494

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

Nelson-Rees and
Flandermeyer, 1977;
Nelson-Rees et al,
1981
Yoshino et al, 2006;
ATCC website
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Unknown

Unknown

557237,6451928
16643607
6451928
6451928

HBT-39b

Human

Breast carcinoma

HeLa

Human

HBT-E
(HBT-3 clone)

Human

Breast carcinoma

HeLa

Human

HCE

Human

Cervical carcinoma

HeLa

Human

HCu-10Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCu-18Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCu-22

Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCu-27

Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCu-33

Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCu-37

Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCu-39Human

Esophageal squamouscell carcinoma

Hcu-10, Hcu-18, Hcu22, Hcu-27, Hcu-33,
Human
Hcu-37 or Hcu-39(genetically identical)

Esophageal squamouscell carcinoma

van Helden et al,1988

3167823

HCV-29Tmv(HCV-29
derivative)

Human

Bladder, tumorigenic
urothelial cells

T-24

Human

Bladder carcinoma

Christensen et al,
1993

8105864

HEC-155

Human

Uterine adenocarcinoma HEC-180

Human

Uterine carcinoma

JCRB website

No PMID

HEC-180

Human

Uterine adenocarcinoma HEC-155

Human

Uterine adenocarcinoma JCRB website

No PMID

HEK

Human

HEK/HRV(HEK derivative)

Human

Kidney, embryonic renal
HeLa
cells
Kidney, transformed
HeLa
embryonic renal cells

Human
Human

6451928
6451928

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Unknown

Nelson-Rees et al,
19816451928,
7236009

Nelson-Rees et al,
1981; Chen, 1988

6451928,3180844

ECACC website

No PMID

ECACC website

No PMID

Korch et al, 2012

22710073

6451928
6451928

HEL-R66

Human

Not specified

Unknown

Monkey, African
green(Cercopithecus
aethiops )HEp-2 (H.Ep.-2)

Human

Laryngeal carcinoma

HeLa

Human

Hep-2C

Human

Laryngeal carcinoma

HeLa

Human

Hep2 (Clone 2B)

Human

Laryngeal carcinoma

HeLa

Human

HES

Human

Endometrium

HeLa

Human

HIMEG-1

Human

Leukemia, chronic
myeloid

HL-60

Human

Leukemia, acute
myeloid, M2

Drexler et al, 2003

12592342

HKB-1

Human

Lymphoma, Hodgkin

Unknown

Human

Unknown

Drexler et al, 2003

12592342

HKMUS

Human

Cervical carcinoma

SKG-II-SF

Human

Cervical carcinoma

RIKEN website

No PMID

HKMUS-SF

Human

Cervical carcinoma

SKG-II-SF

Human

Cervical carcinoma

Yoshino et al, 2006

16643607

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

Page 5

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

HL111783

Human

Lung carcinoma

HeLa

Human

Yoshino et al, 2006

16643607

HMV-1

Human

Melanoma

HeLa

Human

HPB-MLT

Human

Leukemia, acutelymphoblastic, T cell

MacLeod et al, 1999

10508494

HPB-ALL

Human

HPC-36M(HPC-36
derivative)

Drexler et al, 2003

12592342

Human

Prostate carcinoma

HeLa

Human

Cervicaladenocarcinoma

Masters et al, 2001

11416159

hPTC

Human

Thyroid, papillary cell

Unknown

Pig, Sus scrofa

Unknown

MacLeod et al, 1999

10508494

Hs 677.St

Human

Gastric tissue, normal

Unknown

Mouse

Unknown

ATCC FAQ #1072

No PMID

HSC-41

Human

Gastric carcinoma

HSC-42

Human

Gastric carcinoma

JCRB website

No PMID

HSG

Human

Salivary gland,
submandibular

HeLa

Human

Cervicaladenocarcinoma

ECACC website

No PMID

HSG-AZA1

Human

HeLa

Human

JCRB website

No PMID

HSG-AZA3

Human

HeLa

Human

JCRB website

No PMID

HSGc-C5

Human

HeLa

Human

JCRB website

No PMID
11732505,
12592342

Salivary gland,
submandibular
Salivary gland,
submandibular
Oral carcinoma

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Leukemia, acutelymphoblastic, T cell

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

HS-SULTAN

Human

MyelomaJIJOYE

Human

Lymphoma, Burkitt

Drexler et al, 2001;
Drexler et al, 2003

HSY

Human

Salivary gland, parotid

HeLa

Human

Cervicaladenocarcinoma

JCRB website

No PMIDhTERT-EEC

Human

Endometrium,
immortalized

MCF-7

Human

Breast carcinoma

Korch et al, 2012

22710073

Hu1734

Human

Bladder, non-malignant
HCV-29urothelial cells

Human

Bladder, non-malignant Christensen et al,urothelial cells
19937905254

Hu456

Human

T-24

Human

Bladder carcinoma

Hu549

Human

T-24

Human

Bladder carcinoma

Hu609

Human

Bladder, non-malignant
J82
urothelial cells

Human

Bladder carcinoma

Hu609Tmv(Hu609 derivative)

Human

Bladder, tumorigenic
urothelial cells

T-24

Human

Bladder carcinoma

Hu961a, Hu961t
Human(Hu961 derivatives)

Bladder, tumorigenic
urothelial cells

T-24

Human

Bladder carcinoma

HeLa

Human

HeLa

Human

HuK 39

o

Human

HuL-1

Human

Bladder, tumorigenic
urothelial cells
Bladder, tumorigenic
urothelial cells

Kidney, normal renal
cells
Liver, hepatocellular
carcinoma

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

Christensen et al,
1993
Christensen et al,
1993
Christensen et al,
1993; Masters et al,
2001
Christensen et al,
1993
Christensen et al,
1993; Masters et al,
2001
Nelson-Rees et al,
19818105864
8105864
7905254,11416159
8105864
8105864,
11416159
6451928JCRB website

No PMID

Nelson-Rees et al,
19816451928

MacLeod et al, 1999

10508494

Masters et al, 2001

11416159

RIKEN website

No PMID
566722,6451928

Hut

Human

Not specified

HeLa

Human

IMC-2

Human

Maxillary carcinoma

HeLa

Human

IMC-3

Human

Maxillary carcinoma

HeLa

Human

IMC-4

Human

Maxillary carcinoma

HeLa

Human

Intestine 407(Int-407, HEI)

Human

Intestinal cells(jejunum/ileum),
embryonic

HeLa

Human

Cervicaladenocarcinoma

Lavappa, 1978;
Nelson-Rees et al,
1981

IPDDC-A2

Human

Astrocytoma

UnknownRat

Unknown

ECACC website

No PMID

IPRB

Human

Glioblastoma

UnknownRat

Unknown

Higgins et al, 2010

20951163

IPTP/98

Human

Glioblastoma

UnknownRat

Unknown

ECACC website

No PMID

IST-1

Human

Placenta

SK-OV-3

Human

Ovarian carcinoma

ATCC FAQ #1068

No PMID

J-111

Human

Leukemia, acute
myeloid, M5

HeLa

Human

Cervicaladenocarcinoma

J96

Human

Leukemia

HeLa

Human

Cervicaladenocarcinoma

JCA-1

Human

Prostate carcinoma

T-24

Human

JHC

Human

Placenta

HeLa

Human

JHU012

Human

Oral squamous cell
JHU022
carcinoma (lymph node)

Human

JHU013 (JHU012
derivative)

Human

Oral squamous cell
FaDu
carcinoma (lymph node)

Human

Bladder carcinoma
Cervicaladenocarcinoma
Laryngeal squamouscell carcinoma (lymph
node)
Hypopharyngeal
squamous cell
carcinoma

Nelson-Rees et al,
1981; Drexler et al,
2003
Nelson-Rees et al,
1981
van Bokhoven et al,
2001
Nelson-Rees et al,
19816451928,
12592342
6451928
11522622
6451928

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

Page 6

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

JHU019Human

JHU028Human

JHT(JHC derivative)

Claimed Cell Type
Oropharyngeal
squamous cell
carcinoma
Oral squamous cell
carcinoma

Contaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

PC-3

Human

Prostate carcinoma

Zhao et al, 2011

21868764

A549

Human

Lung carcinoma

Zhao et al, 2011

21868764

Nelson-Rees et al,
19816451928
21868764

Placenta

HeLa

Human

Cervicaladenocarcinoma

JMAR
Human
(Tu-167 derivative)

Oral squamous cell
carcinoma

UM-SCC-1

Human

Oral squamous cell
carcinoma (recurrence)

Zhao et al, 2011

JOSK-I

Human

Leukemia, acute
myeloid, M4

U-937

Human

Lymphoma, histiocytic

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

JOSK-K

Human

Leukemia, acute
myeloid, M5

U-937

Human

Lymphoma, histiocytic

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

JOSK-M

Human

Leukemia, chronic
myeloid, blast crisis

U-937

Human

Lymphoma, histiocytic

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

JOSK-S

Human

Leukemia, acute
myeloid, M5

U-937

Human

Lymphoma, histiocytic

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

JTC-17

Human

Skin

HeLa

Human

JTC-3

Human

Not specified

HeLa

Human

K051

Human

Leukemia, acute
myeloid, M2

K-562

Human

K1

Human

Thyroid, papillarycarcinoma

GLAG-66

K2

Human

Thyroid, papillarycarcinoma

KAK1

Human

KAT10

Human

Honma et al, 1992;
JCRB website

1730567

Ogura et al, 1997

9556756

Leukemia, chronic
myeloid, blast crisis

Drexler et al, 2003

12592342

Human

Thyroid, papillarycarcinoma

Ribeiro et al, 2008;
19087340,
Schweppe et al, 2008 18713817

GLAG-66

Human

Thyroid, papillarycarcinoma

Ribeiro et al, 2008;
19087340,
Schweppe et al, 2008 18713817

Thyroid, follicularadenoma

HT-29

Human

Colon carcinoma

Human

Thyroid, papillarycarcinoma

HT-29

Human

Colon carcinoma

KAT4

Human

Thyroid, anaplastic
carcinoma

HT-29

Human

Colon carcinoma

KAT5

Human

Human

Colon carcinoma

Schweppe et al, 2008 18713817

KAT50

Human

Human

Colon carcinoma

Schweppe et al, 2008 18713817

KAT7

Human

Human

Colon carcinoma

Schweppe et al, 2008 18713817

KB

Human

Oral carcinoma

HeLa

Human

Cervicaladenocarcinoma

Gartler, 1967;
Lavappa et al, 1976;
Nelson-Rees et al,
19814864103,
1250349,
6451928

KB-3-1

Human

Oral carcinoma

HeLa

Human

DSMZ website

No PMID

KB-V1

Human

Oral carcinoma

HeLa

Human

DSMZ website

No PMID

KCI-MOH1

Human

Pancreatic carcinoma

HPAC

Human

Pancreatic carcinoma

DSMZ website

No PMID

KM-3

Human

Leukemia, acutelymphoblastic, B cell
precursor

REH

Human

Leukemia, acutelymphoblastic, B cell
precursor

Drexler et al, 2003

12592342

KM3

Human

Melanoma

UnknownRat

Not specified

Moseley et al, 2003

12740908

KMS-21-BM

Human

MyelomaUnknown

Human

Unknown

Drexler et al, 2003

12592342

KMT-2

Human

Umbilical cord blood

KG-1

Human

Leukemia

Yoshino et al, 2006

16643607

KNS-89Human

Gliosarcoma

U-251 MG

Human

Glioblastoma

JCRB website

No PMID

KOSC-3

Human

Oral carcinoma

Ca9-22

Human

Oral carcinoma

JCRB website

No PMID

KP-1N

Human

Pancreatic carcinoma

PANC-1

Human

Pancreatic carcinoma

JCRB website

No PMID

KPB-M15

Human

Leukemia, chronic
myeloid, blast crisis

KYO-1

Human

Leukemia, chronic
myeloid, blast crisis

Drexler et al, 2003

12592342

KPL-1

Human

Breast carcinoma

MCF-7

Human

Breast carcinoma

DSMZ website

No PMID

KP-P1

Human

Prostate carcinoma

HeLa

Human

Cervicaladenocarcinoma

Nelson-Rees et al,
19816451928

KSY-1

Human

Sarcoma (Kaposi)

T-24

Human

Bladder carcinoma

Stürzl et al, 2012;
ATCC FAQ #1061

22987579

KU7

Human

Bladder carcinoma

HeLa

Human

Cervicaladenocarcinoma

Jäger et al, 2013

23500642

Thyroid, papillary
HT-29
carcinoma
Thyroid, differentiated
HT-29
cells
Thyroid, benign follicular
HT-29
hyperplasia

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Cervicaladenocarcinoma
Cervicaladenocarcinoma

van Staveren et al,17804723,
2007; Schweppe et al,
18713817
2008
van Staveren et al,17804723,
2007; Schweppe et al,
18713817
2008
van Staveren et al,17804723,
2007; Schweppe et al,
18713817
2008

Page 7

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

KU-YS

Human

Neuroblastoma

KU-SN

Human

Misidentification
Reported By

Neuroectodermal tumor RIKEN website

Reference
PubMed ID
No PMID

L-132

Human

Lung cells, embryonic

HeLa

Human

Cervicaladenocarcinoma

Lavappa, 1978;
Nelson-Rees et al,
1981

L-41
(J96 derivative)

Human

Leukemia, bone marrow HeLa

Human

Cervicaladenocarcinoma

ECACC website

No PMID

LED-Ti

Human

Cervical carcinoma

HeLa

Human

Cervicaladenocarcinoma

Nelson-Rees et al,
19816451928

LLC-15MB

Human

Breast carcinoma

M14

Human

Melanoma

Thompson et al, 2004 15679051

Human

Glioma, anaplastic
astrocytoma

LN-992

Human

Glioblastoma

Bady et al, 2012

22570425

Bady et al, 2012

22570425

LN-319Glioblastoma,
recurrence (different
donor)
Leukemia, acutelymphoblastic, B cell
precursor

566722,6451928

LN-443

Human

Glioblastoma

LN-444

Human

LR10.6

Human

Leukemia, acutelymphoblastic, B cell
precursor

NALM-6

Human

LT-1

Frog, grass

Kidney, renal
adenocarcinoma

TH and FHM

TH = box turtle;
FHM = fatheadminnow

LU

HumanLung cells, fetal

HeLa

Human

LU 106

Human

Lung cells, embryonic

HeLa

Human

Lu-130

Human

Lung carcinoma

Lu-134A, B

HumanLung carcinoma

Yoshino et al, 2006

16643607

Nelson-Rees et al,
19816451928

No PMID

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

TH = heart; FHM =unspecified

Nelson-Rees et al,
1981

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

M10T

Human

Synovial cell

HeLa

Human

Cervicaladenocarcinoma

M4A4 andderivatives(MDA-MB-435
derivatives)

Human

Breast carcinoma

M14

Human

Melanoma

ATCC website

MA-1

Human

Lymphoma, MALT

Pfeiffer

Human

Lymphoma, nonHodgkin

Capes-Davis et al,
2013
Whitaker andHayward, 1985;
Milanesi et al, 2003;
ATCC FAQ #1067

Monkey, African
green(Cercopithecus
aethiops )Monkey, African
green(Cercopithecus
aethiops )MA-104

Monkey, Rhesus
Kidney, embryonic renal(MacacaVero?
cellsmulatta )MA-111

RabbitKidney, newborn

Vero?

MA-160Human

Prostate adenoma

HeLa

Human

MaTu

Human

Breast carcinoma

HeLa

Human

MC-4000

Human

Breast carcinoma

HeLa

Human

Kidney, normal renalcells

6451928
6451928
645192823907998

14505435

Kidney, normal renalcells

Nelson-Rees et al,
19816451928

Cervicaladenocarcinoma

Nelson-Rees, 1977;
Nelson-Rees et al,
1981

562836,6451928

MacLeod et al, 1999

10508494

MacLeod et al, 1999

10508494
6451928

Cervicaladenocarcinoma
Cervicaladenocarcinoma

McCoy

Human

Not specified

Strain L

Mouse

Connective tissue

Nelson-Rees et al,1981

MCF-7/AdrR(NCI/ADR-RES)

HumanBreast carcinoma

OVCAR-8

Human

Ovarian carcinoma

Liscovitch and Ravid,
16504380
2007

MDA-MB-435

Human

Breast carcinoma

M14

Human

Melanoma

Ellison et al, 2002;
12354931,
Christgen et al, 2007; 17786032,
Rae et al, 2007
17004106

MDA-N(MDA-MB-435
derivative)

Human

Breast carcinoma,
HER2/ERBB2transfected

M14

Human

Melanoma

Lorenzi et al, 2009

19372543

MDS

Human

Leukemia, chronic
myelomonocytic

JURKAT

Human

Leukemia, acutelymphoblastic, T cell

Drexler et al, 2003

12592342

MEL-HO

Human

Melanoma

Unknown, does notmatch other cell linefrom same donor

Human

Unknown

MacLeod et al, 1999

10508494

ME-WEI

Human

Melanoma

Unknown, does notmatch other cell linefrom same donor

Human

Unknown

MacLeod et al, 1999

10508494

MGH-U1 (EJ)

Human

Bladder carcinoma

T-24

Human

Bladder carcinoma

MGH-U2 (HM)

Human

Bladder carcinoma

T-24

Human

Bladder carcinoma

O'Toole et al, 1983;
Lin et al, 1985
O'Toole et al, 1983;
Lin et al, 1985

6823318,40279866823318,4027986

MHH-225

Human

Leukemia, acute
myeloid, M7

JURKAT

Human

Leukemia, acutelymphoblastic, T cell

Drexler et al, 2003

12592342

Minnesota EE

Human

Esophageal epithelium

HeLa

Human

Cervicaladenocarcinoma

Lavappa, 1978;
Nelson-Rees et al,
1981

566722,6451928

MKB-1

Human

Leukemia, acute
myeloid

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

Page 8

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

MKN28

Human

Gastric carcinoma

MKN74

Human

Gastric carcinoma

JCRB website

No PMID

Gastric carcinoma

Unknown

Human

Lymphoblastoid cells

Suzuki and Sekiguchi, No PMID (book1999
chapter)

Human

Melanoma

Mash-1

Human

Schwannoma

Yoshino et al, 2006

16643607

MOBS-1

Human

Leukemia, acute
myeloid, M5

U-937

Human

Lymphoma, histiocytic

Drexler et al, 2003

12592342

MOLT-15

Human

Leukemia, acutelymphoblastic, T cell

CTV-1

Human

Leukemia, acute
myeloid, M5

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

MPanc-96

Human

Pancreatic carcinoma

AsPC-1

Human

Pancreatic carcinoma

ATCC website

HT-29

Human

Colon carcinoma

Schweppe et al, 2008 18713817

MKN-7

Human

MMAc

MRO87-1

HumanMS(Monkey Stable)

Monkey

MT-1

Human

MUTZ-1Thyroid, follicular
carcinoma
Kidney, normal renal
cells

HeLa

Human

Breast carcinoma

HeLa

Human

Human

Leukemia, acute
myeloid, M2

Namalwa

Human

MV522

Human

Lung carcinoma

HT-29

NC-37

Human

Lymphoblastoid cellline, normal donor

NCC16

Human

NCI/ADR-RES(MCF-7/AdrR)

Cervicaladenocarcinoma
Cervicaladenocarcinoma

No PMID

Milanesi et al, 2003

14505435

MacLeod et al, 1999

10508494

Lymphoma, Burkitt

Drexler et al, 2009

19344951

Human

Colon carcinoma

ATCC FAQ #1075

No PMID

Raji

Human

Lymphoma, Burkitt

JCRB website

No PMID

Cervical carcinoma

PHK16-0b

HumanSkin, immortalised
keratinocytes

JCRB website

No PMID

Human

Breast carcinoma

OVCAR-8

Human

Ovarian carcinoma

Liscovitch and Ravid,
16504380
2007

NCI-H1264

Human

Lung carcinoma

NCI-H157

Human

Lung carcinoma

ATCC FAQ #930,#931

No PMID

NCI-H1304

Human

Lung carcinoma, smallcell

NCI-H1870

Human

Lung carcinoma, small
cell

ATCC FAQ #1069

No PMID

NCI-H1514

Human

Lung carcinoma

Unknown

Human

Unknown

NCI-H157

Human

Lung carcinoma

NCI-H1264

Human

Lung carcinoma

NCI-H1622

Human

Lung carcinoma

Unknown

Human

Unknown

ATCC website

No PMID

NCI-H1870

Human

Lung carcinoma, smallcell

NCI-H1304

Human

Lung carcinoma, small
cell

ATCC FAQ #1069

No PMID

NCI-H249

Human

Lung carcinoma, smallcell

NCI-H69

Human

Lung carcinoma, small
cell

ATCC FAQ #1073

No PMID

NCI-H513

Human

Mesothelioma

NCI-H125

Human

Lung carcinoma

ATCC FAQ #1060

No PMID

NCI-H592

Human

Lung carcinoma, smallcell

NCI-H69

Human

Lung carcinoma, small
cell

ATCC FAQ #1070,#1073

No PMID

NCI-H60

Human

Lung carcinoma, small
cell

NCI-N417

Human

Lung carcinoma, small
cell

ATCC FAQ #1064

No PMID

NCI-H630

Human

Metastatic sample

MixtureHuman

Unknown

ATCC FAQ #1065

No PMID

NCI-H738

Human

Lung carcinoma

Unknown

Human

Unknown

ATCC website

No PMID

Human

Intestinal cells, normal
colon

LoVo

Human

Colon carcinoma

Melcher et al, 2005

15771911

NCOL-1NCTC 2544

Human

Skin epithelium

HeLa

Human

Cervicaladenocarcinoma

NCTC 3075

Human

Not specified

HeLa

Human

Cervicaladenocarcinoma

Durkin et al, 2000;
ATCC website
ATCC FAQ #930,#931

Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,
1981
van Bokhoven et al,200110949990
No PMID

566722,6451928566722,6451928
11304728

ND-1

Human

Prostate carcinoma

DU-145

Human

Prostate carcinoma

NM2C5 andderivatives(MDA-MB-435
derivatives)

Human

Breast carcinoma

M14

Human

Melanoma

ATCC website

No PMID

NOI-90Human

Lymphoma, nonHodgkin, natural killer
cell

REH

Human

Leukemia, acutelymphoblastic, B cell
precursor

Drexler et al, 2003

12592342

NOSE06

Human

Ovary, normal

DOV-13

Human

Ovarian carcinoma

Korch et al, 2012

22710073

NOSE07

Human

Ovary, normal

DOV-13

Human

Ovarian carcinoma

Korch et al, 2012

22710073

NPA87

Human

Thyroid, papillarycarcinoma

M14

Human

Melanoma

Schweppe et al, 2008; 18713817,
Zhao et al, 2011
21868764

NS-3

Human

Gastric carcinoma

COLO 201

Human

Colon carcinoma

JCRB website

No PMID

Page 9

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

OCUM-6

Human

Gastric carcinoma

OCUM-11

Human

Gastric carcinoma

JCRB website

No PMID
6451928,
95567569556756

OE

Human

Endometrium

HeLa

Human

Cervicaladenocarcinoma

Nelson-Rees et al,
1981; Ogura et al,
1997

OF

Human

Not specified

HeLa

Human

Cervicaladenocarcinoma

Ogura et al, 1997

OLGA-PH-J/92[OL-J/92]

Crayfish,Orconectes
limosus

Brain, cerebral ganglion Unknown

Unknown, similar
to amoeba
Unknown
species

Lee et al, 2011; ATCC
21938590
website

ONCO-DG-1

Human

Thyroid, papillarycarcinoma

Human

Ovarian carcinoma

DSMZ website

No PMID

Cervicaladenocarcinoma

Personal
communication, R.
Nardone

No PMID

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

Drexler et al, 2003

12592342

OST

Human

OU-AML-1

Human

OU-AML-2

Human

OU-AML-3

Human

OU-AML-4

Human

OU-AML-5

Human

OU-AML-6

Human

OU-AML-7

Human

OU-AML-8

Human

OV-1063

Human

OV2008,OV2008/C13

Sarcoma(osteosarcoma)

OVCAR-3
HeLa

Human

OCI/AML2

Human

OCI/AML2

Human

OCI/AML2

Human

OCI/AML2

Human

OCI/AML2

Human

OCI/AML2

Human

OCI/AML2

Human

OCI/AML2

Human

Ovarian carcinoma

Unknown

Human

Unknown

ATCC website

No PMID

Human

Ovarian carcinoma

ME-180

Human

Cervical carcinoma

Korch et al, 2012

22710073

Ovary1847

Human

Ovarian carcinoma

NIH:OVCAR-8

Human

Ovarian carcinoma

Korch et al, 2012

22710073

OVMIU

Human

Ovarian carcinoma

OVSAYO

Human

Ovarian carcinoma

JCRB website

No PMID

P1-1A3

Human

Thymic epithelium

SK-HEP-1

Human

Liver carcinoma

MacLeod et al, 1999

10508494

P1-4D6

Human

Thymic epithelium

SK-HEP-1

Human

Liver carcinoma

MacLeod et al, 1999

10508494

P39/TSUGANE(P39/TSU)

Human

Leukemia, acute
myeloid, M2

HL-60

Human

Leukemia, acute
myeloid, M2

Drexler et al, 2003;
JCRB website

12592342

PBEI

Human

Leukemia, acutelymphoblastic, B cell
precursor

NALM-6

Human

PC-93

Human

Prostate carcinoma

HeLa

Human

PC-MDS

Human

Myelodysplastic
syndrome, therapyrelated

K-562

Human

PCI-22A

Human

Oral squamous cell
carcinoma

Unknown, does notmatch other cell linefrom same donor

PCI-22B

Human

PCI-3

Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M2
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M2
Leukemia, acute
myeloid, M5
Leukemia, acute
myeloid, M1
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4

Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4

Leukemia, acutelymphoblastic, B cell
precursor
Cervicaladenocarcinoma

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342
van Bokhoven et al,200314518029

Leukemia, chronic
myeloid, blast crisis

Drexler et al, 2009

19344951

Human

Unknown

Zhao et al, 2011

21868764

Unknown, does not
Oral squamous cell
match other cell line
carcinoma (lymph node)from same donor

Human

Unknown

Zhao et al, 2011

21868764

Human

Oropharyngeal
squamous cell
carcinoma

PC-3

Human

Prostate carcinoma

Zhao et al, 2011

21868764

PEAZ-1

Human

Prostate carcinoma

HT-1080

Humanvan Bokhoven, 2004;
11433418
Sarcoma (fibrosarcoma) Schmelz et al, 2001
[erratum][erratum]PH61-N

Human

Not specified

MIA PaCa-2

Human

Pancreatic carcinoma

JCRB website

No PMID

PLB-985

Human

Leukemia, acute
myeloid, M4

HL-60

Human

Leukemia, acute
myeloid, M2

Drexler et al, 2003

12592342

PPC-1

Human

Prostate carcinoma

PC-3

Human

Prostate carcinoma

Chen, 1993

8428522

PSV811

Human

Skin fibroblast, Werner's
WI-38
syndrome

HumanLung, normal diploidfibroblasts

JCRB website

No PMID

RAMAK-1

Human

Muscle synovium

Human

Bladder carcinoma

T-24

MacLeod et al, 1999

10508494
6451928,
12592342
10508494

RB

Human

Lymphoma, Hodgkin

OMK-210

Monkey, Owl
Kidney, normal renal(Aotus trivirgatus ) cells

Nelson-Rees et al,
1981; Drexler et al,2003

RBHF-1

Human

Liver, hepatoma

Unknown

Non-human

MacLeod et al, 1999

Unknown

Page 10

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

RC-2A

Human

Leukemia, acute
myeloid, M4

CCRF-CEM

Human

RED-3

Human

HL-60

REH-6

Human

RERF-LC-MA

Human

RERF-LC-OK

Human

RGC-5

Misidentification
Reported By

Reference
PubMed ID

Leukemia, acutelymphoblastic, T cell

Drexler et al, 2003

12592342

Human

Leukemia, acute
myeloid, M2

Drexler et al, 2003

12592342

Unknown

Mouse

Unknown

Drexler et al, 2003

12592342

Lung carcinoma, small
cell

SK-MES-1

HumanLung, squamous cell

Personal
communication, A.
Capes-Davis

No PMID

Lung carcinoma

MarcusHuman

Astrocytoma

JCRB website

No PMID

Mouse

Unknown

Van Bergen et al,2009

19443730

Drexler et al, 2003

12592342

Leukemia, acute
myeloid
Leukemia, acutelymphoblastic, B cell
precursor

Rat

Retinal ganglion

RM-10

Human

Leukemia, chronic
myeloid, blast crisis

Unknown
K-562

Human

Leukemia, chronic
myeloid, blast crisis

RMG-I

Human

Ovarian carcinoma

RMG-II

Human

Ovarian carcinoma

JCRB website

No PMID

RMUG-L

Human

Ovarian carcinoma

SNG-II

Human

Endometrial carcinoma

JCRB website

No PMID

RO-D81-1

Human

Thyroid, medullarycarcinoma

HT-29

Human

Colon carcinoma

Dadon et al, 2013

23472229

RO-H85-1

Human

Thyroid, medullarycarcinoma

647-V

Human

Bladder carcinoma

Dadon et al, 2013

23472229

Personal
communication, A.
Capes-Davis

No PMID

RPMI-4788

Human

Colon carcinoma

HeLa

Human

Cervicaladenocarcinoma

RPMI-6666

Human

Lymphoma, Hodgkin

Correct name,
incorrect cell type

Human

EBV+ B-lymphoblastoid
Drexler et al, 2003
cell line

12592342

RPTC-1

Human

Thyroid, papillarycarcinoma

TPC-1

Human

Thyroid, papillarycarcinoma

Zhao et al, 2011

21868764

RS-1

Human

Leukemia, acute
myeloid, M7

K-562

Human

Leukemia, chronic
myeloid, blast crisis

Drexler et al, 2003

12592342

RTSG

Human

Ovarian carcinoma

SNG-II

Human

Endometrial carcinoma

RY

Human

Lymphoma, Hodgkin

SA4

Human

Sarcoma (liposarcoma) HeLa

Human

SAM-1

Human

Leukemia, chronic
myeloid, blast crisis

K-562

Human

SAML-1

Human

Lymphoma, Hodgkin

U-937

Human

SBC-2

Human

SBC-7

Human

Unknown

MonkeyBladder carcinoma

HeLa

Human

Bladder carcinoma

HeLa

Human

SCCKN

Human

SCLC-21/22H

Human

SCLC-21/22H

Human

Oral squamous cell
carcinoma
Lung carcinoma, small
cellLung carcinoma, small
cell

Unknown
Cervicaladenocarcinoma
Leukemia, chronic
myeloid, blast crisis
Lymphoma, histiocytic
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Oral squamous cell
carcinoma
Lung carcinoma, small
cellLung carcinoma, small
cell

JCRB website

No PMID

Harris et al, 1981;
Drexler et al, 2003
Nelson-Rees et al,
19817192801,
12592342

Drexler et al, 2003

12592342

ATCC FAQ #1074

No PMID

MacLeod et al, 1999

10508494

MacLeod et al, 1999

10508494

Yoshino et al, 2006

16643607

MacLeod et al, 1999

10508494

MacLeod et al, 1999

10508494

6451928

SCCTF

Human

SCLC-16H

Human

SCLC-24H

Human

SEG-1

Human

Esophageal
adenocarcinoma

NCI-H460

Human

Lung carcinoma, large
cell

Boonstra et al, 2010

20075370

SF767

Human

Glioma, anaplastic
astrocytoma

ME-180

Human

Cervical carcinoma

Bady et al, 2012

22570425

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Unknown

Silversides et al, 2010 20200241

SH-2

Human

Breast carcinoma

HeLa

SH-3

Human

Breast carcinoma

HeLa

Human
Human

6451928
6451928

SJPL

Pig, Sus scrofa

Lung cells, immortalized
Unknownepithelial

Monkey, African
green(Cercopithecus
aethiops )SK-GT-5

Human

Esophageal
adenocarcinoma

SK-GT-2

Human

Gastric carcinoma(gastric fundus)

Boonstra et al, 2010

20075370

SK-MG-1

Human

Astrocytoma

MarcusHuman

Astrocytoma

JCRB website

No PMID

SK-N-MC

Human

Neuroblastoma

Unknown

Human

Sarcoma (Ewing's)

Staege et al, 2004

15548687

SK-OV-4

Human

Ovarian carcinoma

C-33A

Human

Cervical carcinoma

Korch et al, 2012

22710073

SK-OV-6

Human

Ovarian carcinoma

C-33A

Human

Cervical carcinoma

Korch et al, 2012

22710073

SKW-3

Human

Leukemia, chronic
lymphocytic, T cell

KE-37

Human

Leukemia, acutelymphoblastic

DSMZ website

No PMID

SLK

Human

Sarcoma (Kaposi)

Caki-1

Human

Renal cell carcinoma

Stürzl et al, 2012

22987579

Page 11

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

SNB-19Human

Glioblastoma

U-251 MG

Human

Azari et al, 2007;
ATCC website

17254797

SPI-801

Human

K-562

Human

SPI-802

Human

K-562

Human

SpR

Human

Lymphoma, Hodgkin

OMK-210

Monkey, Owl
Kidney, normal renal(Aotus trivirgatus ) cells

SQ-5

Human

Lung carcinoma

HeLa

Human

Human

Leukemia, acutelymphoblastic, T cell

AML-193

Human

SR-91

Leukemia, acutelymphoblastic, T cell
Leukemia, acutelymphoblastic, T cell

Glioblastoma
Leukemia, chronic
myeloid, blast crisis
Leukemia, chronic
myeloid, blast crisis

Cervicaladenocarcinoma
Leukemia, acute
myeloid, M5Gignac et al, 1993;
Drexler et al, 2003
Gignac et al, 1993;
Drexler et al, 2003
Nelson-Rees et al,
1981; Drexler et al,
2003

8220135,
12592342
8220135,
12592342

Yoshino et al, 2006

16643607

Drexler et al, 2003

12592342

6451928,
12592342

Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,7394535,
1980; Nelson-Rees et6451928al, 1981

SW-527

Human

Colon carcinoma

SW-480, SW-620

Human

Colon carcinoma

SW-598

Human

Colon carcinoma

SW-480, SW-620

Human

Colon carcinoma

SW-608

Human

Colon carcinoma

SW-480, SW-620

Human

Colon carcinoma

SW-613

Human

Colon carcinoma

SW-480, SW-620

Human

Colon carcinoma

SW-732

Human

Colon carcinoma

SW-480, SW-620

Human

Colon carcinoma

SW-733

Human

Colon carcinoma

SW-480, SW-620

Human

Colon carcinoma

T-1

Human

Kidney, normal renal
cells

HeLa

Human

Cervicaladenocarcinoma

T1

Human

Neural stem cell

HeLa

Human

Cervicaladenocarcinoma

Torsvik et al, 2012

22949886

T-33

Human

Leukemia, chronic
myeloid, blast crisis

K-562

Human

Leukemia, chronic
myeloid, blast crisis

Drexler et al, 2003

12592342

Human

Oral squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

T406 (oral SCC)**Different cell line
exists with same
Human
name: T406
(glioblastoma)**Oral squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

T409

Human

Oral squamous cell
carcinoma

UM-SCC-1

Human

Oral squamous cell
carcinoma (recurrence)

Zhao et al, 2011

21868764

T-9(WI-38 derivative)

Human

Lung cells, transformed
HeLa
normal diploidfibroblasts

Human

Cervicaladenocarcinoma

Nelson-Rees et al,
19816451928

TCO-1

Human

Cervical carcinoma

Human

Cervical carcinoma

Yoshino et al, 2006

16643607

T404

TDL-1

Human

TDL-2

Human

TCO-2

Tonsillar lymphoid cells,
P3JHR-1
non-neoplastic
Tonsillar lymphoid cells,
P3JHR-1
non-neoplastic
Tonsillar lymphoid cells,
RPMI 1788
non-neoplastic
Tonsillar lymphoid cells,
Rajinon-neoplastic

Human

Lymphoma, Burkitt

Human

Lymphoma, Burkitt

Human

Lymphoblastoid cellline, normal donor

Human

Lymphoma, Burkitt

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
19816451928
6451928
6451928

TDL-3

Human

TDL-4

Human

TE-12

Human

Esophageal squamouscell carcinoma

TE-2, TE-3, TE-12 orTE-13 (geneticallyHuman
identical)

Esophageal squamouscell carcinoma

Boonstra et al, 2007

17804709

TE-13

Human

Esophageal squamouscell carcinoma

TE-2, TE-3, TE-12 orTE-13 (geneticallyHuman
identical)

Esophageal squamouscell carcinoma

Boonstra et al, 2007

17804709

TE-2

Human

Esophageal squamouscell carcinoma

TE-2, TE-3, TE-12 orTE-13 (geneticallyHuman
identical)

Esophageal squamouscell carcinoma

Boonstra et al, 2007

17804709

TE-3

Human

Esophageal squamouscell carcinoma

TE-2, TE-3, TE-12 orTE-13 (geneticallyHuman
identical)

Esophageal squamouscell carcinoma

Boonstra et al, 2007

17804709

TE671

Human

Medulloblastoma

RD

Human

Human

Medulloblastoma

RD

Human

Stratton et al, 1989;
Chen et al, 1989
ECACC website,
ATCC FAQ #1063

2650908,
2739733

TE671 Subline
No.2

Sarcoma(rhabdomyosarcoma)
Sarcoma(rhabdomyosarcoma)

TE-7

Human

Esophageal
adenocarcinoma

TE-2, TE-3, TE-12 orTE-13 (geneticallyHuman
identical)

Esophageal squamouscell carcinoma

Boonstra et al, 2007

17804709

6451928

No PMID

Page 12

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell Type

Misidentification
Reported By

Reference
PubMed ID

TEC61

Human

Thyroid, endothelium

JEG3

Human

Choriocarcinoma

Patel et al, 2003
[retraction]

12388152[retraction]

TK-1

Human

Glioblastoma

U-251 MG

Human

Glioblastoma

JCRB website

No PMID

TI-1

Human

Leukemia, acute
myeloid, M2

K-562

Human

Rush et al, 2002;
Drexler et al, 2003

11871388,
12592342

TMH-1

Human

Thyroid, benign goitre

IHH-4

Human

Leukemia, chronic
myeloid, blast crisis
Thyroid, papillary
thyroid carcinoma

JCRB website

No PMID

TMM

Human

Leukemia, chronic
myeloid, blast crisis

Correct name,
incorrect cell type

Human

EBV+ B-lymphoblastoid
Drexler et al, 2003
cell line

12592342

TSU-Pr1

Human

Prostate carcinoma

T-24

Human

Bladder carcinoma

van Bokhoven et al,
2001

11522622

Human

Oral squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

Human

Oropharyngeal
squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

Tu-159Human

Oropharyngeal
squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

Tu-167

Human

Oral squamous cell
carcinoma

UM-SCC-1

Oral squamous cell
carcinoma (recurrence)

Zhao et al, 2011

21868764

Human

Oropharyngeal
squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

Human

Hypopharyngeal
squamous cell
carcinoma

T404, T406, Tu-138,
Tu-158LN, Tu-159,
Tu-182, Tu-212, Tu- Human
212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

Tu-212LN

Human

T404, T406, Tu-138,
Hypopharyngeal
Tu-158LN, Tu-159,
squamous cell
Tu-182, Tu-212, Tu- Human
carcinoma (lymph node) 212LN (genetically
identical)

Head and necksquamous cell
carcinoma

Zhao et al, 2011

21868764

TuWi

Human

Kidney, Wilms' tumor

HeLa

Human

Cervicaladenocarcinoma

Lavappa, 1978;
Nelson-Rees et al,
19816451928

U-118 MG

Human

Glioblastoma

U-138 MG

Human

Glioblastoma

ATCC website

No PMID

Human

Bladder carcinoma

Chiong et al, 2009

19375735

Tu-138

Tu-158LN

Tu-182

Tu-212

Human

UM-UC-2

Human

Bladder carcinoma

T-24

UM-UC-3-GFP(UM-UC-3
derivative)

Human

Bladder carcinoma,
GFP-transfected

Unknown, NOT UMHumanUC-3

Unknown

Chiong et al, 2009

19375735

UPES/C

Human

Brain, non-neoplastic
astrocytes

UnknownRat

Unknown

Higgins et al, 2010

20951163

UPHHJA

Human

Glioblastoma

UnknownRat

Unknown

Higgins et al, 2010

20951163

UTMB-460

Human

B-cells

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

Drexler et al, 2003

12592342

VC312R

Human

Medulloblastoma

Unknown

Mixed, human +mouse

Unknown

Higgins et al, 2010

20951163

VM-CUB-III

Human

Bladder carcinoma

VM-CUB-I

Human

Bladder carcinoma

Masters et al, 1986

3708594

WiDr

Human

Colon carcinoma

HT-29

Human

Colon carcinoma

Chen et al, 1987

3472642566722,6451928

WISH

Human

Amnion, normal cells

HeLa

Human

Cervicaladenocarcinoma

Lavappa, 1978;
Nelson-Rees et al,
1981

WKD

Human

Conjunctiva

HeLa

Human

Cervicaladenocarcinoma

ECACC website

No PMID

Cervicaladenocarcinoma

Lavappa, 1978;
Nelson-Rees et al,
1981

566722,6451928

Wong-Kilbourne

Human

Conjunctiva

HeLa

Human

Page 13

Version 7.1 Table 1
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell TypeContaminating
Cell Line

Actual Species Actual Cell TypeWRL 68Human

Liver, embryonic cells

HeLa

Human

WSU-ALCL

Human

Lymphoma, anaplastic
large cell

CCRF-CEM

Human

WSU-CLL

Human

Leukemia, chronic
lymphocytic

REH

Human

YAA

Human

Monocytes

U-937

Human

YAP

Human

Monocytes

U-937

Human

Leukemia, chronic
myelomonocytic

YJ

HL-60

Misidentification
Reported By

Reference
PubMed ID

ECACC website

No PMID

Drexler et al, 2003

12592342

Drexler et al, 2002;
Drexler et al, 2003

12200708,
12592342

Lymphoma, histiocytic

Drexler et al, 2003

12592342

Human

Lymphoma, histiocytic

Drexler et al, 2003

12592342

Human

Leukemia, acute
myeloid, M2

Drexler et al, 2003

12592342

Cervicaladenocarcinoma
Leukemia, acutelymphoblastic, T cell
Leukemia, acutelymphoblastic, B cell
precursor

YMB-1

Human

Breast carcinoma

ZR-75-1

Human

Breast carcinoma

YMB-1-E

Human

Breast carcinoma

ZR-75-1

Human

Breast carcinoma

Personal
communication, A.
Capes-Davis
Personal
communication, A.
Capes-Davis

No PMID

No PMID

Page 14

Version 7.1 Table 2
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

The database of cross-contaminated or misidentified cell lines consists of two tables. Table 1 (see previous pages) contains those cell lines where there is no authentic
stock known to the list contributors.
Table 2 (below) contains cell lines where some stocks have been shown to be misidentified, but where authentic stocks are known to exist.
To help scientists obtain authentic stocks, an additional column in this table shows possible locations for authentic stock. If supplied by a cell line repository, thecatalogue number is given in brackets. Repositories are listed in alphabetical order.

Table 2. Misidentified cell lines where authentic stock is known to exist
Contaminating
Cell Line

ActualSpecies

Authentic
Actual Cell Type Stock
Location

Misidentification Reference
Reported By
PubMed ID

HumanPeter G. Sacks,
Oral squamous cell
New York
carcinoma
University(recurrence)(originator)

Zhao et al, 2011

21868764

Peter G. Sacks,
New York
University(originator)

Zhao et al, 2011

21868764

Misidentified
Cell Line

ClaimedSpecies

Claimed Cell
Type

1483

Human

Oral squamous cell
UM-SCC-1
carcinoma

183

Human

Oropharyngeal
squamous cell
carcinoma

T404, T406, Tu138, Tu-158LN, Tu159, Tu-182, TuHuman
212, Tu-212LN(genetically
identical)

Head and necksquamous cell
carcinoma

207 (EU-2)

Human

Leukemia, acutelymphoblastic, B
cell precursor

REH and SUP-B2

Human

Leukemia, acutelymphoblastic, B
cell precursor

B10XI

Chicken,Gallus gallus

Intestinal cells

Unknown

Pig, Sus
scrofaUnknown

Univeristy of
Magdeburg,
Germany

Steube et al, 2012

22193509

B6

Chicken,Gallus gallus

Intestinal cells,
small intestine

Unknown

Pig, Sus
scrofaUnknown

Univeristy of
Magdeburg,
Germany

Steube et al, 2012

22193509

BJA-B

Human

Bladder carcinoma HeLa

Human

Cervicaladenocarcinoma

BT-20

Human

Breast carcinoma

Human

HeLa

Cervicaladenocarcinoma
Laryngeal
squamous cell
carcinoma(recurrence)

MacLeod et al, 1999; 10508494,
Drexler et al, 2003
12592342

Nelson-Rees &1246601
Flandermeyer, 1976
ATCC (HTB-19)

Nelson-Rees et al,
19816451928JCRB(JCRB0625);
RIKEN(RCB1976)

Zhao et al, 2011

21868764

Korch et al, 2012;
ICLAC
correspondence

22710073

O'Donoghue et al,
2011

21908323

Ca9-22

Human

Oral squamous cell
MSK-922
carcinoma

Human

COLO-720E

Human

COLO684,
Ovarian carcinoma COLO685,
COLO704

Human

Uterineadenocarcinoma

D-17Dog, Canisfamiliaris

Sarcoma(osteosarcoma)

Dog, Canisfamiliaris

Sarcoma(osteosarcoma)

Human

ATCC (CCL-85);
Lymphoma, Burkitt ECACCJCRB website(90121003)

No PMID

FU-RPNT-1

Human

Kidney, renal
primitive
neuroectodermal
tumor

RIKEN website

No PMID

Unknown

Human

Unknown

O'Donoghue et al,
2011

21908323

JURKAT

Human

Leukemia, acutelymphoblastic, T
cell

Drexler et al, 2003

12592342

Unknown

Cow

Unknown

Steube et al, 2012

22193509

Human

Bladder carcinoma

Masters et al, 1986

3708594

Unknown

Mouse

Unknown

JCRB(JCRB1062)

JCRB website

No PMID

Unknown

Human

Unknown

DSMZ (ACC105)

Drexler et al, 1999

10516762

HL-60

Human

Drexler et al, 2003

12592342

CCRF-CEM

Human

DSMZ (ACC-46) Drexler et al, 1999

10516762

DSMZ (ACC-72) Drexler et al, 2003

12592342

Drexler et al, 1999

10516762

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

EB-3

Human

FU-RPNT-2

Human

Grey

Dog, Canisfamiliaris

HPB-ALL

Human

IPEC

Pig, Sus
scrofa

J-82

Human

MorescoLymphoma, Burkitt Daudi
Kidney, renal
primitive
neuroectodermaltumor
Sarcoma(osteosarcoma)
Leukemia, acutelymphoblastic, T
cell
Intestinal cells

Bladder carcinoma T-24
Liver,
hepatocellular
carcinoma
Leukemia, acutelymphoblastic, T
cell
Leukemia, acute
myeloid, M4
Leukemia, acutelymphoblastic, T
cell

JHH-1

Human

KARPAS-45

Human

KBM-3

Human

KE-37

Human

L-540

Human

Lymphoma,
Hodgkin

CCRF-CEM

Human

MB-02

Human

Leukemia, acute
myeloid, M7

HU-3

Human

MDA1986LN

Human

Oral squamous cell
carcinoma (lymph UM-SCC-1
node)

Human

MDA686LN

Human

Oropharyngeal
squamous cell
carcinoma (lymph
node)

Human

UM-SCC-1

Leukemia, acute
myeloid, M2
Leukemia, acutelymphoblastic, T
cell
Leukemia, acutelymphoblastic, T
cell
Leukemia, acute
myeloid, M7

ATCC (CCL-183)

RIKEN(RCB2078)

DSMZ (ACC483); RIKEN(RCB1935)
Univeristy of
Magdeburg,
GermanyPeter G. Sacks,
Oral squamous cell
New York
carcinoma
University(recurrence)(originator)
Peter G. Sacks,
Oral squamous cell
New York
carcinoma
University(recurrence)(originator)

Page 15

Version 7.1 Table 2
Publication Date 22/08/2013

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified
Cell Line

MDA686TU

ActualSpecies

Authentic
Actual Cell Type Stock
Location

Misidentification Reference
Reported By
PubMed ID

ClaimedSpecies

Claimed Cell
Type

Contaminating
Cell Line

Human

Oropharyngeal
squamous cell
carcinoma

T404, T406, Tu138, Tu-158LN, Tu159, Tu-182, TuHuman
212, Tu-212LN(genetically
identical)

Head and necksquamous cell
carcinoma

MacLeod et al, 2013 23319659

O'Donoghue et al,
2011

21908323

Drexler et al, 1999

10516762

Nelson-Rees et al,
19816451928

RIKEN website

No PMID

ECACC website;
ATCC website

No PMID

Peter G. Sacks,
New York
University(originator)

Zhao et al, 2011

21868764

NCI-H929

Human

Myeloma

K-562

Human

ATCC (CRLLeukemia, chronic 9068); DSMZ
myeloid, blast(ACC-163);
crisis
ECACC(95050415)

Parks

Dog, Canisfamiliaris

Melanoma

Unknown

Human

Unknown

RPMI-8402

Human

Leukemia, acutelymphoblastic, T
cell

Unknown

Human

Unknown

RT4

Human

Bladder carcinoma HeLa

Human

Cervicaladenocarcinoma

T3M-12

Human

Lung carcinoma

T3M-1

Human

Oral carcinoma

U-373 MG

Human

Glioblastoma

U-251 MG

Human

Glioblastoma

ECACC(08061901)

10516762

Drexler et al, 2003

12592342

O'Donoghue et al,
2011

21908323

U-937

Human

UT-7

Human

YamadaDog, Canisfamiliaris

Lymphoma,histiocytic

Leukemia, acute
myeloid, M7
Sarcoma(osteosarcoma)

DSMZ (ACC290)
ATCC (HTB-2);
DSMZ (ACC412); ECACC(91091914)
RIKEN(RCB2281)

Unknown

Human

Unknown

ATCC (CRL1593.2); DSMZ
(ACC-5); ECACC
Drexler et al, 1999
(85011440);JCRB(JCRB9021)

U-937

Human

Lymphoma,histiocytic

DSMZ (ACC137)

Unknown

Mouse

Unknown

Page 16

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Version 7.1 References
Publication Date 22/08/2013

Cell Bank Websites Cited
Name
ATCC:
American Type
Culture Collection

Websitehttp://www.atcc.org/

DSMZ:
Deutsche Sammlungvon Mikroorganismen http://www.dsmz.de/und Zellkulturen
GmbH
ECACC:
European Collectionof Cell Cultures

http://www.hpacultures.org.uk/collections/ecacc.jsp

JCRB:
Japanese Collectionof Research
Bioresources

http://cellbank.nibio.go.jp/

RIKEN Bioresource
Center Cell Bank

http://www.brc.riken.go.jp/lab/cell/english/guide.shtml

References
PubMed ID
17254797
22570425
17804709

20075370
21253487

Reference
Azari S, Ahmadi N, Tehrani MJ, Shokri F. Profiling and authentication of human cell lines using short tandem repeat (STR) loci: Report from the
National Cell Bank of Iran. Biologicals 2007; 35: 195-202.
Bady P, Diserens AC, Castella V, Kalt S, Heinimann K, Hamou MF, Delorenzi M, Hegi ME. DNA fingerprinting of glioma cell lines andconsiderations on similarity measurements. Neuro Oncol 2012; 14: 701-11.
Boonstra JJ, van der Velden AW, Beerens EC, van Marion R, Morita-Fujimura Y, Matsui Y, Nishihira T, Tselepis C, Hainaut P, Lowe AW, Beverloo
BH, van Dekken H, Tilanus HW, Dinjens WN. Mistaken identity of widely used esophageal adenocarcinoma cell line TE-7. Cancer Res 2007; 67:7996-8001.
Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang
LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. Verification and unmasking of widely used human esophageal
adenocarcinoma cell lines. J Natl Cancer Inst 2010; 102: 271-4.
Brüderlein S, Sommer JB, Meltzer PS, Li S, Osada T, Ng D, Möller P, Alcorta DA, Kelley MJ. Molecular characterization of putative chordoma celllines. Sarcoma 2010; 2010: 630129.

23907998

Capes-Davis A, Alston-Roberts C, Kerrigan L, Reid YA, Barrett T, Burnett EC, Cooper JR, Freshney RI, Healy L, Kohara A, Korch C, Masters JR,
Nakamura Y, Nims RW, Storts DR, Dirks WG, Macleod RA, Drexler HG. Beware imposters: MA-1, a novel MALT lymphoma cell line, ismisidentified and corresponds to Pfeiffer, a diffuse large B-cell lymphoma cell line. Genes Chromosomes Cancer 2013, Epub Aug 1.

8428522

Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet Cell Genet 1993; 62: 183-4.

2739733

Chen TR, Dorotinsky C, Macy M, Hay R. Cell identity resolved. Nature 1989; 340: 106.

3472642
19375735
8105864
7905254
17786032
23472229
10614862

Chen TR, Drabkowski D, Hay RJ, Macy M, Peterson W Jr. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet
Cytogenet 1987; 27: 125-34.
Chiong E, Dadbin A, Harris LD, Sabichi AL, Grossman HB. The Use of Short Tandem Repeat Profiling to Characterize Human Bladder Cancer Cell
Lines. J Urol 2009; 181: 2737-48.
Christensen B, Hansen C, Debiec-Rychter M, Kieler J, Ottensen S, Schmidt J. Identity of tumorigenic human urothelial cell lines and'spontaneously' transformed sublines. Br J Cancer 1993; 68: 879-84.
Christensen B, Hansen C, Kieler J, Schmidt J. Identity of non-malignant human urothelial cell lines classified as transformation grade I (TGrI) and II(TGrII). Anticancer Res 1993; 13: 2187-91.
Christgen M, Lehmann U. MDA-MB-435: the questionable use of a melanoma cell line as a model for human breast cancer is ongoing. Cancer
Biol Ther 2007; 6: 1355-7.
Dadon T, Ball D, Nelkin BD. Misidentification of Putative Medullary Thyroid Cancer Cell Lines RO-H85-1 and RO-D81-1. J Clin Endocrinol Metab
2013; 98: 954-5.
Dirks WG, MacLeod RA, Drexler HG. ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross-contamination at source. In Vitro Cell
Dev Biol Anim 1999; 35: 558-9.

7682880

Drexler HG. Recent results on the biology of Hodgkin and Reed-Sternberg cells. II. Continuous cell lines. Leuk Lymphoma 1993; 9: 1-25.

10516762

Drexler HG, Dirks WG, MacLeod RA. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 1999; 13:
1601-7.

19344951

Drexler HG, Dirks WG, Macleod RA. Many are called MDS cell lines: One is chosen. Leuk Res 2009; 33: 1011-6.

12592342

Drexler HG, Dirks WG, Matsuo Y, MacLeod RA. False leukemia–lymphoma cell lines: an update on over 500 cell lines. Leukemia 2003; 17: 41626.

11732505

Drexler HG, MacLeod RA, Dirks WG. Cross-contamination: HS-Sultan is not a myeloma but a Burkitt lymphoma cell line. Blood 2001; 98: 3495-6.

12200708

Drexler HG, Quentmeier H, Dirks WG, Uphoff CC, MacLeod RA. DNA profiling and cytogenetic analysis of cell line WSU-CLL reveal crosscontamination with cell line REH (pre B-ALL). Leukemia 2002; 16: 1868-70.

10949990

Durkin AS, Cedrone E, Sykes G, Boles D, Reid YA. Utility of gender determination in cell line identity. In Vitro Cell Dev Biol Anim 2000; 36: 344-7.

20959409
12354931

Ellis LM, Samuel S, Sceusi E. Varying Opinions on the Authenticity of a Human Midgut Carcinoid Cell Line - Letter. Clin Cancer Res 2010; 16:5365-6.
Ellison G, Klinowska T, Westwood RF, Docter E, French T, Fox JC. Further evidence to support the melanocytic origin of MDA-MB-435. Mol
Pathol 2002; 55: 294-9.

4864103

Gartler, SM. Genetic markers as tracers in cell culture. Natl Cancer Inst Monogr 1967; 26: 167-95.

8220135

Gignac SM, Steube K, Schleithoff L, Janssen JW, MacLeod RA, Quentmeier H, Drexler HG. Multiparameter approach in the identification of crosscontaminated leukemia cell lines. Leuk Lymphoma 1993; 10: 359-68.

4402510

Greene AE, Charney J, Nichols WW, Coriell LL. Species identity of insect cell lines. In Vitro 1972; 7: 313-22.

Page 17

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

PubMed ID
7192801
20951163
1730567
23500642
11869090
22710073

Version 7.1 References
Publication Date 22/08/2013

Reference
Harris NL, Gang DL, Quay SC, Poppema S, Zamecnik PC, Nelson-Rees WA, O'Brien SJ. Contamination of Hodgkin's disease cell cultures.
Nature 1981; 289: 228-30.
Higgins SC, Steingrimsdottir H, Pilkington GJ. Human, mouse or rat? Species authentication of glioma-derived cell cultures. J Neurosci Methods
2010; 194: 139-43.
Honma M, Kataoka E, Ohnishi K, Ohno T, Takeuchi M, Nomura N, Mizusawa H. A new DNA profiling system for cell line identification for use incell banks in Japan. In Vitro Cell Dev Biol 1992; 28A: 24-8.
Jäger W, Horiguchi Y, Shah J, Hayashi T, Awrey S, Gust KM, Hadaschik BA, Matsui Y, Anderson S, Bell RH, Ettinger S, So AI, Gleave ME, Lee IL,
Dinney CP, Tachibana M, McConkey DJ, Black PC. Hiding in plain view: Genetic profiling reveals decades old cross-contamination of bladdercancer cell line KU7 with HeLa. J Urol 2013, Epub Mar 7.
Kniss DA, Xie Y, Li Y, Kumar S, Linton EA, Cohen P, Fan-Havard P, Redman CW, Sargent IL. ED(27) trophoblast-like cells isolated from firsttrimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype. Placenta 2002; 23: 32-43.
Korch C, Spillman MA, Jackson TA, Jacobsen BM, Murphy SK, Lessey BA, Jordan VC, Bradford AP. DNA profiling analysis of endometrial andovarian cell lines reveals misidentification, redundancy and contamination. Gynecol Oncol 2012; 127: 241-8.

1250349

Lavappa KS, Macy ML, Shannon JE. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 1976; 259: 211-3.

566722

Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 1978; 14: 469-475.

21938590
4027986
16504380
19372543
10508494
9447816
370859411416159
2903855

Lee LE, Bufalino MR, Christie AE, Frischer ME, Soin T, Tsui CK, Hanner RH, Smagghe G. Misidentification of OLGA-PH-J/92, believed to be theonly crustacean cell line. In Vitro Cell Dev Biol Anim 2011; 47: 665-674.
Lin CW, Lin JC, Prout GR Jr. Establishment and characterization of four human bladder tumor cell lines and sublines with different degrees ofmalignancy. Cancer Res 1985; 45: 5070-9.
Liscovitch M, Ravid D. A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived fromOVCAR-8 human ovarian carcinoma cells. Cancer Lett 2007; 245: 350-2.
Lorenzi PL, Reinhold WC, Varma S, Hutchinson AA, Pommier Y, Chanock SJ, Weinstein JN. DNA fingerprinting of the NCI-60 cell line panel. Mol
Cancer Ther 2009; 8: 713-24.
MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, Drexler HG. Widespread intraspecies cross-contamination of human tumor cell lines
arising at source. Int J Cancer 1999; 83: 555-63.
MacLeod RA, Dirks WG, Reid YA, Hay RJ, Drexler HG. Identity of original and late passage Dami megakaryocytes with HEL erythroleukemia cells
shown by combined cytogenetics and DNA fingerprinting. Leukemia 1997; 11: 2032-8.
Masters JR, Hepburn PJ, Walker L, Highman WJ, Trejdosiewicz LK, Povey S, Parkar M, Hill BT, Riddle PR, Franks LM. Tissue culture model oftransitional cell carcinoma: characterization of twenty-two human urothelial cell lines. Cancer Res 1986; 46: 3630-6.
Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A,
Ward TH, Ayres KL, Debenham PG. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc Natl
Acad Sci U S A 2001; 98: 8012-7.
Matsuba I, Lernmark A, Madsen O, Michelsen B, Nielsen JH, Scholler J, Vissing H, Welinder B, Tommerup N, Mikkelsen M, et al. Gene probes todetect cross-culture contamination in hormone producing cell lines. In Vitro Cell Dev Biol 1988; 24: 1071-6.

15771911

Melcher R, Maisch S, Koehler S, Bauer M, Steinlein C, Schmid M, Kudlich T, Schauber J, Luehrs H, Menzel T, Scheppach W. SKY and genetic
fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1. Cancer Genet Cytogenet 2005; 158: 84-7.

14505435

Milanesi E, Ajmone-Marsan P, Bignotti E, Losio MN, Bernardi J, Chegdani F, Soncini M, Ferrari M. Molecular detection of cell line crosscontaminations using amplified fragment length polymorphism DNA fingerprinting technology. In Vitro Cell Dev Biol Anim 2003; 39: 124-30.

12740908

Moseley GW, Elliott J, Wright MD, Partridge LJ, Monk PN. Interspecies contamination of the KM3 cell line: implications for CD63 function inmelanoma metastasis. Int J Cancer 2003; 105: 613-6.

562836

Nelson-Rees WA. MA160 and HeLa cell lines. In Vitro 1977; 13: 525.

535908

Nelson-Rees WA. Prostatic acid phosphatase in cell lines MA 160, EB33 and HeLa cells. In Vitro 1979; 15: 935.

6451928

Nelson-Rees WA, Daniels DW, Flandermeyer RR. Cross-contamination of cells in culture. Science 1981; 212: 446-52.

1246601

Nelson-Rees WA, Flandermeyer RR. HeLa cultures defined. Science 1976; 191: 96-8.

7394535

Nelson-Rees WA, Flandermeyer RR, Daniels DW. T-1 cells are HeLa and not of normal human kidney origin. Science 1980; 209: 719-20.

21908323
9556756
8397027682331811135436
12388152
7579375
19002917
19557180
17004106
19087340
11871388
11433418
18713817
20200241

O'Donoghue LE, Rivest JP, Duval DL. Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line
validation. J Vet Diagn Invest 2011; 23: 780-785.
Ogura H, Fujii R, Hamano M, Kuzuya M, Nakajima H, Ohata R, Mori T. Detection of HeLa cell contamination--presence of human papillomavirus
18 DNA as HeLa marker in JTC-3, OG and OE cell lines. Jpn J Med Sci Biol 1997; 50: 161-7.
Ogura H,Yoshinouchi M,Kudo T,Imura M,Fujiwara T,Yabe Y. Human papillomavirus type-18 DNA in so-called HEP-2-cells. KB-cells and F1-cells further evidence that these cells are. HeLa-cell derivatives. Cell Mol Biol (Noisy-le-grand) 1993; 39: 463-7.
O'Toole CM, Povey S, Hepburn P, Franks LM. Identity of some human bladder cancer cell lines. Nature 1983; 301: 429-30.
Pan Y, Lui WO, Nupponen N, Larsson C, Isola J, Visakorpi T, Bergerheim US, Kytölä S. 5q11, 8p11, and 10q22 are recurrent chromosomal
breakpoints in prostate cancer cell lines. Genes Chromosomes Cancer 2001; 30: 187-95.
Patel VA, Logan A, Watkinson JC, Uz-Zaman S, Sheppard MC, Ramsden JD, Eggo MC. Isolation and characterization of human thyroid
endothelial cells [retraction]. Am J Physiol Endocrinol Metab 2009; 297: E562.
Pellat-Deceunynk C, Amiot M, Bataille R, Van Riet I, Van Camp B, Omede P, Boccadoro M. Human myeloma cell lines as a tool for studying thebiology of multiple myeloma: a reappraisal 18 years after. Blood 1995; 86: 4001-2.
Perry GM, McDonald GJ, Ferguson MM, Ganassin RC, Bols NC. Characterization of rainbow trout cell lines using microsatellite DNA profiling.
Cytotechnology 2001; 37: 143-51.
Phuchareon J, Ohta Y, Woo JM, Eisele DW, Tetsu O. Genetic profiling reveals cross-contamination and misidentification of 6 adenoid cystic
carcinoma cell lines: ACC2, ACC3, ACCM, ACCNS, ACCS and CAC2. PLoS One 2009; 4: e6040.
Rae JM, Creighton CJ, Meck JM, Haddad BR, Johnson MD. MDA-MB-435 cells are derived from M14 melanoma cells--a loss for breast cancer,
but a boon for melanoma research. Breast Cancer Res Treat 2007; 104: 13-9.
Ribeiro FR, Meireles AM, Rocha AS, Teixeira MR. Conventional and molecular cytogenetics of human non-medullary thyroid carcinoma:characterization of eight cell line models and review of the literature on clinical samples. BMC Cancer 2008; 8: 371.
Rush LJ, Heinonen K, Mrózek K, Wolf BJ, Abdel-Rahman M, Szymanska J, Peltomäki P, Kapadia F, Bloomfield CD, Caligiuri MA, Plass C.
Comprehensive cytogenetic and molecular genetic characterization of the TI-1 acute myeloid leukemia cell line reveals cross-contamination with K562 cell line. Blood 2002; 99: 1874-6.
Schmelz M, Cress AE, Barrera J, McDaniel KM, Davis TL, Fuchs L, Dalkin BL, Nagle RB. PEAZ-1: a new human prostate neoplastic epithelial cellline [erratum]. Prostate. 2004; 61: 375.
Schweppe RE, Klopper JP, Korch C, Pugazhenthi U, Benezra M, Knauf JA, Fagin JA, Marlow LA, Copland JA, Smallridge RC, Haugen BR.
Deoxyribonucleic Acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy andmisidentification. J Clin Endocrinol Metab 2008; 93: 4331-41.
Silversides DW, Music N, Jacques M, Webby R, Gagnon CA. Species origin investigation of the St. Jude porcine lung epithelial cell line (SJPL)
made available to researchers. J Virol 2010; 84: 5454-5.

Page 18

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

PubMed ID
15548687
22193509
4640072265090822987579
No PMID
15679051
22949886
12453433
19443730
11522622
14518029
No PMID
316782312619888

Version 7.1 References
Publication Date 22/08/2013

Reference
Staege MS, Hutter C, Neumann I, Foja S, Hattenhorst UE, Hansen G, Afar D, Burdach SE. DNA microarrays reveal relationship of Ewing family
tumors to both endothelial and fetal neural crest-derived cells and define novel targets. Cancer Res 2004; 64: 8213-21.
Steube KG, Koelz AL, Uphoff CC, Drexler HG, Kluess J, Steinberg P. The necessity of identity assessment of animal intestinal cell lines: A casereport. Cytotechnology 2012; 64: 373-8.
Stoolmiller AC, Dorfman A, Sato GH. Rat origin of CHB cells. Science 1972; 178: 1308.
Stratton MR, Darling J, Pilkington GJ, Lantos PL, Reeves BR, Cooper CS. Characterization of the human cell line TE671. Carcinogenesis 1989;
10: 899-905.
Stürzl M, Gaus D, Dirks WG, Ganem D, Jochmann R. Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant froma known renal carcinoma cell line. Int J Cancer 2013; 132: 1954-8.
Suzuki, T., Sekiguchi, M. (1999). Gastric cancer. In: Human Cell Culture Vol II, Masters JRW & Palsson, B, eds., Kluwer Academic Publishers,
Dordrecht, pp257-291.
Thompson EW, Waltham M, Ramus SJ, Hutchins AM, Armes JE, Campbell IG, Williams ED, Thompson PR, Rae JM, Johnson MD, Clarke R.
LCC15-MB cells are MDA-MB-435: a review of misidentified breast and prostate cell lines. Clin Exp Metastasis 2004; 21: 535-41.
Torsvik A, Røsland GV, Bjerkvig R. Spontaneous transformation of stem cells in vitro and the issue of cross-contamination. Int J Biol Sci 2012; 8:1051-2; discussion 1053-4.
Unger RE, Krump-Konvalinkova V, Peters K, Kirkpatrick CJ. In Vitro Expression of the Endothelial Phenotype: Comparative Study of Primary
Isolated Cells and Cell Lines, Including the Novel Cell Line HPMEC-ST1.6R. Microvasc Res 2002; 64: 384-97.
Van Bergen NJ, Wood JP, Chidlow G, Trounce IA, Casson RJ, Ju WK, Weinreb RN, Crowston JG. Recharacterization of the RGC-5 retinal
ganglion cell line. Invest Ophthalmol Vis Sci 2009; 50: 4267-72.
van Bokhoven A, Varella-Garcia M, Korch C, Miller GJ. TSU-Pr1 and JCA-1 cells are derivatives of T24 bladder carcinoma cells and are not of
prostatic origin. Cancer Res 2001; 61: 6340-4.
van Bokhoven A, Varella-Garcia M, Korch C, Johannes WU, Smith EE, Miller HL, Nordeen SK, Miller GJ, Lucia MS. Molecular characterization ofhuman prostate carcinoma cell lines. Prostate 2003; 57: 205-25.
van Bokhoven A. Models for prostate cancer: molecular characterization and critical appraisal of human prostate carcinoma cell lines. PhD thesis
2004. University of Nijmegen, Nijmegen, The Netherlands.
van Helden PD, Wiid IJ, Albrecht CF, Theron E, Thornley AL, Hoal-van Helden EG. Cross-contamination of human esophageal squamous
carcinoma cell lines detected by DNA fingerprint analysis. Cancer Res 1988; 48: 5660-2.
van Pelt JF, Decorte R, Yap PS, Fevery J. Identification of HepG2 variant cell lines by short tandem repeat (STR) analysis. Mol Cell Biochem
2003; 243: 49-54.

17804723

van Staveren WC, Solís DW, Delys L, Duprez L, Andry G, Franc B, Thomas G, Libert F, Dumont JE, Detours V, Maenhaut C. Human thyroid tumor
cell lines derived from different tumor types present a common dedifferentiated phenotype. Cancer Res 2007; 67: 8113-20.

11433521

Varella-Garcia M,Boomer T,Miller GJ. Karyotypic similarity identified by multiplex-FISH relates four prostate adenocarcinoma cell lines: PC-3, PPC1, ALVA-31, and ALVA-41. Genes Chromosomes Cancer 2001; 31: 303-15.

4043530

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev Biol Stand 1985; 60: 125-31.

6244232

Williams RD. Human urologic cancer cell lines. Invest Urol 1980; 17: 359-63.

8795574

Wilson AP, Dent M, Pejovic T, Hubbold L, Radford H. Characterisation of seven human ovarian tumour cell lines. Br J Cancer 1996; 74: 722-7.

16643607
21868764

Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y. Essential role for gene profiling analysis in the authentication ofhuman cell lines. Hum Cell 2006; 19: 43-8.
Zhao M, Sano D, Pickering CR, Jasser SA, Henderson YC, Clayman GL, Sturgis EM, Ow TJ, Lotan R, Carey TE, Sacks PG, Grandis JR, Sidransky
D, Heldin NE, Myers JN. Assembly And Initial Characterization Of A Panel Of 85 Genomically Validated Cell Lines From Diverse Head And Neck
Tumor Sites. Clin Cancer Res 2011; 17: 7248.

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