Optosplit II

One Camera Solution
Dual Emission Image Splitter

1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Multi-Wavelength Imaging

Low Light Imaging

Features and Benefits

Dual Emission Image Splitter• Single camera
Cost effective - only one camera required

The Optosplit II image splitter is a simple and elegant device for dividing an image into• Variable internal path separation
Minimizing the introduction of aberrations

a single camera chip.

• Dichroic mirror and emission filters mounted in areadily interchangeable cube
Exchange filter sets both easily and quickly. Some
competing products have factory fitted filters• Variable and locking rectangular diaphragm
aperture for defining field size
Define the ROI both horizontally and vertically and set
the images to the optimum size for the camera sensor• Compact design with integral C-mount input andoutput ports
Integral C-mounts allow it to be easily attached toa wide variety of standard microscopes and CCDcameras• Simple and precise controls for image registration
Split images can be accurately and easily centredin the desired field of view, and pixels aligned withrespect to each other• Interchangeable filter/dichroic holders for dual andsingle wavelength imaging
Flexibility to use multiple wavelengths by simply
changing filter and re-sizing the defined field• Aperture diaphragms to balance signal levels if
appropriate
Acts as an adjustable neutral density filter, which canbe more convenient than using neutral density filters• Rotating filter mount for polarization studies
Accurately orientates the emission polarization tomaximize the contrast

two separate, spatially equivalent, components that can be displayed side by side on

Splitting is usually performed on the basis of wavelength, allowing applications suchas ratiometric calcium imaging or FRET, however, polarizing beamsplitters are alsosupported.
The two images can be captured simultaneously offering a major benefit over manualor electronic filter changers. A rectangular aperture is used to define the region to beimaged, with a set of simple controls allowing the user to vary the relative positionsof the two output images on the camera. The Optosplit II can significantly widen thescope of any fluorescence imaging system.
The Optosplit II includes controls to allow the two images to be positioned accurately
and conveniently within the camera frame. Images can be acquired using any imaging
software and processed either manually off-line or using an appropriate analysis toolsuch as the Splitview module in MetaMorph (Molecular Devices) or Field Split in AndoriQ.

Key Applications
Ratiometric calcium and pH imaging
Dual probe imaging
Fluorescence Resonance Energy Transfer (FRET)
Total Internal Reflectance Fluorescence (TIRF)
Polarization Studies1

Optosplit II1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

One Camera Solution
Dual Emission Image Splitter

Creating The Optimum
Product for You
How to customize the Optosplit II:
Step 1.
Select the Optosplit II product code.
Step 2.
Please indicate the filter set yourequire.
Step 3.

Step 1.
Choose the Optosplit II using the following product code:
TR-OPTS-20B		

1.0x magnification. TR-OPTS-20B suits the larger sensor size of the						Zyla 4.2, Zyla 5.5, Neo 5.5 and iXon Ultra 888. This Optosplit II is 		suitable for all imaging cameras from Andor, ‘one size fits all’.

Step 2.
The following filter sets are available:

Please indicate which accessories
are required.

Part CodeShort Description

TR-EMFS-F01

GFP/RFP

Semrock FF01-514/30-25, FF02-617/73,
Dichroic FF580-FDi01-25x36

Items shipped with your Optosplit II

TR-EMFS-F02

CFP/YFP

Semrock FF01-475/28, FF01-550/49-25,
Dichroic FF509-FDi01-25x36

TR-EMFS-F05

CAMELEONS

TR-EMFS-F07

GFP/YFP

TR-EMFS-F08

680/732 Filter Set

Semrock FF01-680/13-25, FF01-732/68-25,
Dichroic FF700-Di01-25x36

TR-EMFS-F09

Cy3-Cy5Semrock FF01-579/34-25, FF01-679/41-25,
Dichroic FF640-FDi01-25x36

1 x rectangular input diaphragm
1 x calibration cube with 50/50 mirror
1 x shutter plate1 x corrector lens & holder
1 x Ex/Em empty cube
1 x ND kit with 4 ND filters

Long Description

Semrock FF01-483/32-25, FF01-542/27-25,
Dichroic FF506-Di02-25x36,
Semrock FF01-497/16-25, FF01-550/32,
Dichroic FF509-FDi01-25x36

Emission / excitation filter cube with integrated
polarizing beamsplitter cube &Rotating Optosplit auxillary component holder with
25mm polarizer (Full width)

TR-EMFS-F10Polarizing Filter set

TR-EMFS-F12

Cy3/Cy5.5

Semrock FF01-579/34-25, FF01-692/40-25,
Dichroic FF640-FDi01-25x36

TR-EMFS-F13

Fluo4/Fura Red

Semrock FF01-530/43-25, Chroma HQ615LP,
Dichroic FF580-FDi01-25x36

TR-EMFS-F14

GFP/Cy5

TR-EMFS-F1550/50 BS Mirror

Chroma 50/50 beamsplitter, 25.2x35.6x1mm laser flat

TR-EMFS-F17

GFP/mCherry

Semrock FF02-525/40-25, FF01-640/40-25,
Dichroic FF580-FDi01-25x36

TR-EMFS-F20

GFP/Cy5Semrock FF01-534/42-25, FF01-655/40-25,
Dichroic FF580-FDi01-25x36

TR-EMFS-F21

GFP/mCherry:wide

Semrock FF01-534/42-25, FF01-641/75-25,
Dichroic FF580-FDi01-25x36

Semrock FF02-525/40, FF01-679/41-25,
Dichroic FF580-FDi01-25x36

Step 3.
The following accessories are available:
TR-OPTS-F00

Optosplit filter cube

Empty filter cube for Optosplit II/III

2

Optosplit II1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

One Camera Solution
Dual Emission Image Splitter

Product Drawings

Recommended Software
Device drivers are included in several commercial imaging packages to assist registration and to allow real-time and off-line ratioing or fluorescenceoverlays. Alternatively, the Optosplit can be used with simple image capture software and the processing carried out manually off-line.
The simple and accessible design makes the Optosplit an excellent platform for alternative applications, such as dual polarization imaging. Whilst
optimized for coupling to a scientific microscope, the Optosplit can also be used with camera lenses or any other system of lenses that produce animage plane of suitable size.

Order Today
Need more information? At Andor we are committed to finding
the correct solution for you. With a dedicated team of technicaladvisors, we are able to offer you one-to-one guidance andtechnical support on all Andor products. For a full listing of ourregional sales offices, please see andor.com/contact
Our regional headquarters are:Europe 				Japan
Belfast, Northern Ireland 			Tokyo
Phone +44 (28) 9023 7126			
Phone +81 (3) 6732 8968
Fax +44 (28) 9031 0792			
Fax +81 (3) 6732 8939
North America				ChinaConnecticut, USA 				Beijing
Phone +1 (860) 290 9211			
Phone +86 (10) 8271 9066
Fax +1 (860) 290 9566			
Fax +86 (10) 8271 9055

FM40523

EMS91062

OHS502497

MOptosplitIISS 0615 R13