Test Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 23 0007

4-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival Date

Approval Date

21-Feb-2023

22-Feb-2023

03-Mar-2023

NotesShikata facility, Mouse, Serology and PCR testing for routine HM

Diagnostic Summary
TestColony

Aspiculuris tetraptera PCR

Shikata facility Room 5 (4106)

Tested++/-?

PDG

210002100021000

Sentinel Mice
Aspiculuris tetraptera PCR

Shikata facility Room 7 (4104)
Sentinel Mice

Helicobacter hepaticus PCR
Helicobacter hepaticus PCR

Shikata facility Room 6 (4105)
Sentinel Mice+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference.

Printed on Mar-03-2023 at 00:50 GMT
Page 1 of 9Test Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 JapanNotes
Shikata facility, Mouse, Serology and PCR testing for routine HMResults approved by Kato, Katsuhiko on 03 Mar 2023

Molecular Diagnostics:
Infectious Disease PCR
242526

27

28

2930

3132

33

12345678910

Helicobacter hepaticus PCR----------

Aspiculuris tetraptera PCR--------+-

Syphacia obvelata PCR----------

34

35

36

37

38

39

40

41

42

43

11

12

13

14

15

16

17

18

1920

Helicobacter hepaticus PCR-+--------

Aspiculuris tetraptera PCR--+-------

Syphacia obvelata PCR----------

444546212223

Helicobacter hepaticus PCR---

Aspiculuris tetraptera PCR---Syphacia obvelata PCR---Infectious Disease PCR Profile: Helicobacter hepaticus PCR
Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive

Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous
DNA or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for thepurpose of determining the presence of PCR inhibitors.The RNA spike control is also used to evaluate thereverse-transcription of RNA. Amplification of spike template indicates that there is no detectable inhibition and theassay is valid.

Rodentibacter pneumotropicus: (former) P. pneumotropica-Jawetz
Rodentibacter heylii: (former) P. pneumotropica-Heyl
Filobacterium rodentium: (former) CAR Bacillus

Printed on Mar-03-2023 at 00:50 GMT
Page 2 of 9

Test Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM
Results approved by Kato, Katsuhiko on 02 Mar 2023

Serology
12345678910

12345678910

ELISA SEND----------ELISA MHV----------ELISA MPUL----------ELISA CPIL----IN-----ELISA LCMV----------ELISA ECTRO----------MAG C. kutscheri----------MAG S.Typhimurium----------IFA CPIL

111213141516171819201112131415161718

19

20

ELISA SEND----------ELISA MHV----------ELISA MPUL----------ELISA CPIL----------ELISA LCMV----------ELISA ECTRO----------MAG C. kutscheri----------MAG S.Typhimurium----------21

2223212223

ELISA SEND---ELISA MHV---ELISA MPUL---ELISA CPIL---ELISA LCMV---ELISA ECTRO---MAG C. kutscheri---

MAG S.Typhimurium---Remarks

Printed on Mar-03-2023 at 00:50 GMT
Page 3 of 9

Test Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shiAdvanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 JapanNotes
Shikata facility, Mouse, Serology and PCR testing for routine HM
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction
with tissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by other
serologic assays or by testing of additional samples from the same source; PDG = pending; QNS = Quantity notsufficient.

FROD: Filobacterium rodentium ((former) CAR bacillus)

Printed on Mar-03-2023 at 00:50 GMT
Page 4 of 9Test Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSex

Additio

Location Infon

UndilutBackSample Type: Serum
1 1

Mouse

Shikata facility Room 3

ICR

13 weeks

Female(5105) Sentinel
2 2

Mouse

Shikata facility Room 3

edICR

13 weeks

Female(5105) Sentinel
3 3

Mouse

Shikata facility Room 2

Mouse

Shikata facility Room 2

ICR

13 weeks

FemaleMouse

Shikata facility Room 1

ICR

13 weeks

FemaleMouse

Shikata facility Room 1

ICR

13 weeks

FemaleMouse

Shikata facility Room 4

ICR

13 weeks

FemaleMouse

Shikata facility Room 4

ICR

13 weeks

FemaleMouse

Shikata facility Room 7

ICR

13 weeks

FemaleMouse

Shikata facility Room 7

ICR

13 weeksFemale

Mouse

Shikata facility Room 6ICR

13 weeks

FemaleMouse

Shikata facility Room 6ICR

13 weeks

FemaleMouse

Shikata facility Room 5ICR

13 weeks

Female

14 14

Mouse

Back

Undilut

Front

Undilut

Back

Undilut

Front

Undilut

Back

Undilut

Front

ed
ICR

13 weeks

Female(4106) Sentinel
Shikata facility Room 5

Undiluted(4105) Sentinel
13 13

Front

ed

(4105) Sentinel
12 12

Undilut

ed(4104) Sentinel
11 11

Backed(4104) Sentinel
10 10

Undilut

ed(5115) Sentinel
9 9

Front

ed(5115) Sentinel
8 8

Undilut

ed(5107) Sentinel
7 7Backed(5107) Sentinel
6 6

Undilut
ed(5106) Sentinel
5 5Front

ed(5106) Sentinel
4 4Undilut

Undilut

BackedICR

13 weeks(4106) Sentinel

FemaleUndiluted

Printed on Mar-03-2023 at 00:50 GMT
Page 5 of 9FrontTest Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 JapanNotes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location InfonUndilut

BackSample Type: Serum (continued)
15 15

Mouse

Shikata facility Room 11ICR

13 weeks

Female(4116) Sentinel
16 16

Mouse

ed

Shikata facility Room 11ICR

13 weeks

Female(4116) Sentinel
17 17

Mouse

Mouse

Shikata facility Room 10

ICR

13 weeks

FemaleMouse

Shikata facility Room 10

ICR

13 weeks

FemaleMouse

Shikata facility Room 9

ICR

13 weeks

FemaleMouse

Shikata facility Room 9

ICR

13 weeks

FemaleMouse

Shikata facility Room 8

ICR

13 weeks

FemaleMouse

Undilut

BackUndilut

Front

Undilut

BackedShikata facility Room 8

ICR

13 weeks

Female(4119) Sentinel
23 23

Front

ed

(4119) Sentinel
22 22

Undiluted(4118) Sentinel
21 21

Backed(4118) Sentinel
20 20

Undiluted(4117) Sentinel
19 19

Front

ed(4117) Sentinel
18 18

Undilut

Undilut

FrontedShikata facility Room 12ICR

13 weeks

Female(3121) Sentinel

Undilut

BackedSample Type: Fecal pellet

24 1

Mouse

Shikata facility Room 3

ICR, etc-Female(5105) Sentinel

PooledBackup to
10:1

25 2

Mouse

Shikata facility Room 3

ICR, etc-Female(5105) Sentinel

PooledFrontup to10:1

26 3

Mouse

Shikata facility Room 2

ICR, etc-(5106) Sentinel

FemalePooledup to
10:1

Printed on Mar-03-2023 at 00:50 GMT
Page 6 of 9BackTest Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location InfonPooledFront

Sample Type: Fecal pellet (continued)
27 4

Mouse

Shikata facility Room 2

ICR, etc-Female(5106) Sentinel

up to
10:1

28 5

Mouse

Shikata facility Room 1

ICR, etc-Female(5107) Sentinel

PooledBackup to
10:1

29 6

Mouse

Shikata facility Room 1

ICR, etc-Female(5107) Sentinel

Pooled

Front

up to
10:130 7Mouse

Shikata facility Room 4

ICR, etc-Female(5115) Sentinel

PooledBackup to
10:131 8

Mouse

Shikata facility Room 4

ICR, etc-Female(5115) Sentinel

PooledFrontup to
10:132 9

Mouse

Shikata facility Room 7

ICR, etc-Female(4104) Sentinel

PooledBackup to
10:1

33 10

Mouse

Shikata facility Room 7

ICR, etc-Female(4104) Sentinel

PooledFrontup to
10:134 11

Mouse

Shikata facility Room 6

ICR, etc-Female(4105) Sentinel

PooledBackup to
10:135 12

MouseShikata facility Room 6

ICR, etc-(4105) Sentinel

FemalePooledup to10:1

Printed on Mar-03-2023 at 00:50 GMT
Page 7 of 9FrontTest Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSex

Additio

Location InfonPooled

BackSample Type: Fecal pellet (continued)
36 13

Mouse

Shikata facility Room 5

ICR, etc-Female(4106) Sentinel

up to
10:1

37 14

Mouse

Shikata facility Room 5

ICR, etc-Female(4106) Sentinel

PooledFrontup to
10:1

38 15

Mouse

Shikata facility Room 11

ICR, etc-Female(4116) Sentinel

PooledBackup to
10:1

39 16

Mouse

Shikata facility Room 11

ICR, etc-Female(4116) Sentinel

Pooled

Front

up to
10:1

40 17

Mouse

Shikata facility Room 10

ICR, etc-Female(4117) Sentinel

PooledBackup to10:141 18

Mouse

Shikata facility Room 10

ICR, etc-Female(4117) Sentinel

PooledFront

up to10:1

42 19

Mouse

Shikata facility Room 9

ICR, etc-Female(4118) Sentinel

PooledBackup to10:1

43 20

Mouse

Shikata facility Room 9

ICR, etc-Female(4118) Sentinel

PooledFront

up to10:1

44 21

Mouse

Shikata facility Room 8

ICR, etc-(4119) Sentinel

FemalePooledup to
10:1

Printed on Mar-03-2023 at 00:50 GMT
Page 8 of 9BackTest Results

2023000588

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Mouse, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAge

Sex

Additio

Location InfonPooledFront

Sample Type: Fecal pellet (continued)
45 22

Mouse

Shikata facility Room 8

ICR, etc-Female(4119) Sentinelup to10:1

46 23

Mouse

Shikata facility Room 12

ICR, etc-(3121) SentinelFemale

Pooledup to10:1

Printed on Mar-03-2023 at 00:50 GMT
Page 9 of 9BackTest Results

2023000589

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Billing Information
Payment Method
Purchase Order

Oriental Yeast Co., Ltd.
PO#: RADS SS 23 0007

4-4-1 Minamisuita
Suita-shi Osaka 564-0043 Japan

Details
Sample(s) from:

Multiple locations

Collection Date

Arrival Date

Approval Date

21-Feb-2023

22-Feb-2023

03-Mar-2023

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM

Diagnostic Summary
TestColony

Tested++/-?

PDG

All results NEGATIVE+ = Positive, +/- = Equivocal, ? = Indeterminate, PDG = Pending
To assure the health status of your research animal colonies, it is essential that you understand the sources, pathobiology,
diagnosis and control of pathogens and other adventitious infectious agents that may cause research interference.

Printed on Mar-03-2023 at 00:49 GMT
Page 1 of 4Test Results

2023000589

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM
Results approved by Kato, Katsuhiko on 03 Mar 2023

Molecular Diagnostics:
Infectious Disease PCR

Syphacia muris PCR

567830313233----Remarks- = Negative; I = Inhibition, +/- = Equivocal; + = Positive

Sample Suitability/Detection of PCR Inhibition:Sample DNA or RNA is spiked with a low-copy number of a exogenous
DNA or RNA template respectively. A spike template-specific PCR assay is used to test for the spike template for thepurpose of determining the presence of PCR inhibitors.The RNA spike control is also used to evaluate thereverse-transcription of RNA. Amplification of spike template indicates that there is no detectable inhibition and theassay is valid.

Rodentibacter pneumotropicus: (former) P. pneumotropica-Jawetz
Rodentibacter heylii: (former) P. pneumotropica-Heyl
Filobacterium rodentium: (former) CAR Bacillus

Printed on Mar-03-2023 at 00:49 GMT
Page 2 of 4

Test Results

2023000589

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM
Results approved by Kato, Katsuhiko on 02 Mar 2023

Serology123430

313233ELISA SEND----ELISA SDAV----ELISA MPUL----ELISA CPIL--IN-

ELISA HANT----

MAG C. kutscheri----

MAG S.Typhimurium----

MAG B.bronchiseptica----

IFA CPIL-Remarks
MFIA/ELISA/IFA Results: - = Negative; +/- = Equivocal; + = Moderate to strong positive; TC = Non-specific reaction
with tissue control. All Assays: IN = positive result interpreted as non-specific because not confirmed by otherserologic assays or by testing of additional samples from the same source; PDG = pending; QNS = Quantity notsufficient.

FROD: Filobacterium rodentium ((former) CAR bacillus)

Printed on Mar-03-2023 at 00:49 GMT
Page 3 of 4

Test Results

2023000589

Order #:LTM Customer ID: 42510

The Jackson Laboratory Japan, Inc.

Okayama University(Monitoring Center, JAX in Japan)
955 Kamibayashi, Ishioka-shi

Advanced Science Research Center, OU

Ibaraki 315-0138 Japan

Shikata Campus
2-5-1 Shikatacho, Kita-ku
Okayama-shi Okayama 700-8558 Japan

Notes
Shikata facility, Rat, Serology and PCR testing for routine HM

Sample Information
NumberCodeSpecies

ColonyStrainAgeSexAdditio

Location InfonUndilut-Sample Type: Serum
1 30

Rat

Shikata facility 3107

Wistar

13 weeks

FemaleSentinel
2 31

Rat

ed

Shikata facility 3108

Wistar

13 weeks

FemaleSentinel
3 32

Rat

Rat-

ed

Shikata facility 3119

Wistar

13 weeks

FemaleSentinel
4 33

Undilut

Undilut-ed

Shikata facility 3119

Wistar

13 weeks

FemaleSentinel

Undilut

Monitored

box

Pooled-Sample Type: Fecal pellet
5 30

Rat

Shikata facility 3107

Wistar-FemaleSentinel

up to
6:1

6 31

Rat

Shikata facility 3108

Wistar, etc-

FemaleSentinel

Pooled-

up to
10:1

7 32

Rat

Shikata facility 3119

Wistar-FemaleSentinel

Pooled-

up to
9:1

8 33

Rat

Shikata facility 3119

Wistar-Sentinel

FemalePooledMonitorup tobox

8:1

Printed on Mar-03-2023 at 00:49 GMT
Page 4 of 4