August 23, 2021
Minowa Shikko Co., Ltd.
11-25 Kudashicho,
Echizen-city,
Fukui Prefecture,Japan

Dear All,
Novel Coronavirus Decreased by More than 99.9%

We would like to inform you that we confirmed antiviral effect of urushi against novel coronavirus (SARS
CoV-2) by applying it on the test plate and measuring virus infectivity titer.
Test Summary
Antiviral effect of urushi against novel coronavirus (SARS-CoV-2) has been verified by the test conductedat General Incorporated Association Japan Textile Products Quality and Technology Center, in which oururushi was applied on a test plate.
The testing procedure applied ‘ISO21702 Measurement of antiviral activity on plastics and other nonporous surfaces’ (Figure 1). The plate applied with urushi and the intact plate were both contacted with thecoronavirus, and after 24 hours, the infectivity titers of virus (the number of viral particles with cellinfectivity) recovered from them were measured. The test has revealed that the novel coronavirus on theurushi-applied plate decreased by more than 99.9% in comparison with the virus on the plate without urushi
on it (Figure 2).
(1) Testing Institution: General Incorporated Association Japan Textile Products Quality and Technology
Center (Government designated inspection agency)(2) Test Method: ISO21702 Measurement of antiviral activity on plastics and other non-porous surfaces(3) Tested Virus: Severe acute respiratory syndrome coronavirus 2 (novel coronavirus)(4) Materials used for the test: Acrylic plate 50mm × 50mm applied with urushi, an intact plate(5) Result: More than 99.9% reduction in the infectivity titer of virus
Figure 2
Image Diagram

Acrylic plate
24 hrs later

331,131 viral particles

25℃ roomtemperature
90% humidity

194,984 viral particles

ComparedAcrylic plate withurushi applied on it

24 hrs later

331,131 viral particles

25℃ roomtemperature
90% humidity

151 viral particles

The same number of novel coronavirus particles was inoculated onto the surface of urushi-applied
acrylic plate and intact plate respectively. Comparing the number of A with B after 24 hours has
resulted in more than 9.9% reduction rate.

Figure 1
3. Antiviral test on products with
non-porous surfaces

Inoculation of test virus suspension~ washing out virus from a sample

1. A test piece (50mm50mm) is taken out.

2. 0.4ml of test virus suspension is inoculated onto
the test piece surface and covered with a cover ﬁlm(40mm 40mm).

*3. The test piece is left to stand still at
25 ℃ and RH of 90% or higher for 24hours to allow the virus to act on it.

4. 10ml of solution is added to wash
over the test piece and recover the virus
from it.
Provided by General Incorporated Association Japan
Textile Products Quality and Technology Center* About handling of the report
We do not permit any unauthorized duplication, unauthorized reproduction or unauthorized secondary useof this published contents including the test result. We also refuse unauthorized links to or from thewebsite on which the report posted.
Please note that the company is in no way concerned with, or responsible for, any third party that
reproduces, uses, or indicates links to or from, the report or contents of the report without our permission.
Please be aware that if you indicate the result of this report in association with specific products, such
indications might be considered as an indication of the therapeutic efficacy or preventive effect on aparticular disease and there is a high possibility that your indication will come into conflict with the laws,
such as the Premiums and Representations Act (Act against Unjustifiable Premiums and Misleading
Representations) and Pharmaceutical Affairs Law (Law for Ensuring the Quality, Efficacy, and Safety ofDrugs and Medical Devices).
The test result has been obtained by using our urushi that is natural and refined by traditional techniques.
Not all urushi products or lacquer products will have the same outcome.

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