GeneAce SYBR™ qPCR Mix α Low ROX
I.

V.

Description

Typical PCR Protocol

GeneAce SYBR™ qPCR Mix α Low ROX is a convenient

Choose an appropriate total reaction volume, depending

2X premix for real-time qPCR using SYBR™ Green I dye.

on the instrument used. (e.g., 50 µl or 25 µl)

This product was optimized for instruments that require alow ROX reference signal for fluorescence normalization

Component

VolumeVolume(Compatible instruments: ABI 7500, Stratagene Mx3000P

2x GeneAce SYBR qPCR Mix α Low ROX

25.0 μl12.5 μl/ 3005 / 4000, etc.).

25 μM each Primers

1.0 μl

0.5 μlThis product provides high specificity and reproducibility

Template

5.0 μl

2.5 μland allows for reducing non-specific amplification by using

d.d.H2O

50.0 μl25.0 μl

up tooptimized buffer components and Hot-Start Gene Taq NT,a chemically modified Taq DNA Polymerase.

Recommended cycler conditions

II. Storage–20°C, protected from light.
This product should be protected from light. After thawing,
the master mix may be stored at 4°C for up to one month

95°C

10 min.

95°C30 sec.

60°C

1 min.

Enzyme activation *3)
45 cycles

Melting curve analysis

or returned to -20°C for long term storage.
Optimum PCR condition depends on primer sequenceIII.

and template DNA and so on.

Components
300 rxns

2 x GeneAce SYBR™
qPCR Mix α Low ROX *1)*3) Ensure that the cycling program includes the DNA polymerase
1.5 ml x 5*1)

It contains Hot-Start Gene Taq NT, dNTP Mixture, Mg2+,
SYBR™ Green I, ROX reference dye, stabilizer.

IV.

Notes

・ The PCR must start with an initial 10 minutesactivation step. Initial 10 minutes incubation at 95ºC is required.

For Licensee Products sold in the Research Field:
This product is provided under an intellectual property license fromLife Technologies Corporation.

The transfer of this product isincubation at 95°C to activate the chemicallyconditioned on the buyer using the purchased product solely inmodified hot-start Taq DNA polymerase.

research conducted by the buyer, excluding contract research or

・ We recommend two-step cycling protocols. (See V.
Typical PCR protocol)
・ This product cannot be used for runs in “Fast”*2)

any fee for service research, and the buyer must not (1) use thisproduct or its components for (a) diagnostic, therapeutic orprophylactic purposes; (b) testing, analysis or screening services, ormode on ABI real time instruments.

information in return for compensation on a per-test basis; or (c)*2) 95°C 20 sec→(95°C 3 sec , 60°C 30 sec) x cycle numbers

manufacturing or quality assurance or quality control, and/or

・ Always ensure that the product has been fully thawed(2)

sell or transfer this product or its components for resale, whether orand mixed before use. Gently mix the mixtures without

not resold for use in research.

creating bubbles.

license to this product for purposes other than as described above,

・ Uracil-N-Glycosylase treatment is unusable on thisproduct.

NIPPON GENE CO., LTD.
www.nippongene.com

For information on purchasing acontact Life Technologies Corporation, 5781 Van Allen Way,
Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

For Research Use Only

GeneAce SYBR™ qPCR Mixα Low ROX
I.

V.

製品説明

GeneAce SYBR™ qPCR Mix α Low ROX は、ROX を用い使用例

ご使用の装置に対応した反応液量でご使用下さい。てウェル間の蛍光シグナルの補正を行うリアルタイム PCR 装置（ABI 7500、Stratagene Mx3000P / 3005 / 4000 等）に対＜反応液（例）＞[ 50 μl 系 ][ 25 μl 系 ]応したインターカレーター法用のリアルタイム PCR 用試薬で2x GeneAce SYBR qPCR Mix α Low ROX

25.0 μl12.5 μl

す。2 x 濃度に予め調製されたマスターミックスが小分け分注

25 μM each Primers

1.0 μl

0.5 μl済みです。

Template

5.0 μl

2.5 μl

本製品は、ホットスタート PCR 用酵素 Hot-Start GeneTaq

d.d.H2O

50.0 μl25.0 μlup to

NT と最適化されたバッファーにより、非特異的増幅を抑制し、＜推奨 PCR サイクル条件＞＊3)

高い特異性と再現性を実現しています。

II. 保存－20℃ (遮光)4℃保存(遮光)も可能ですが、その場合は１ヶ月以内にご使

95℃

10 min.

95℃

30 sec.

60℃

1 min.

45 cycles

融解曲線解析

用下さい。
プライマーの設計や鋳型 DNA 等により反応の至適条件が変III.

わることがあります。

製品内容
試 薬

300 反応用

2 x GeneAce SYBR™
qPCR Mix α Low ROX *1)

1.5 ml x 5 本＊1)＊3)酵素の活性化ステップは、95℃10 分間必ず行って下さい。

Hot-Start Gene Taq NT, dNTP Mixture, Mg2+, SYBR™ GreenⅠ,
ROX Reference Dye, stabilizer を含んでいます。

For Licensee Products sold in the Research Field:

IV.

注意

・ 化学修飾を施したホットスタート PCR 用酵素を用いている

This product is provided under an intellectual property license from
Life Technologies Corporation.

The transfer of this product is

ため、酵素活性化ステップ（95℃ 10min.）を必ず実施してconditioned on the buyer using the purchased product solely in下さい。

research conducted by the buyer, excluding contract research or・ 推奨 PCR サイクル条件にてご使用下さい。（V.使用例 参照）

any fee for service research, and the buyer must not (1) use this・ ABI リアルタイム PCR 装置のランモード「Fast」＊2)にはproduct or its components for (a) diagnostic, therapeutic or対応しておりません。
＊2) 95℃ 20 sec →(95℃ 3 sec , 60℃ 30 sec)×ｻｲｸﾙ数

・ 使用時は、泡立てないように穏やかに転倒混和し、試薬を十分均一にしてからご使用下さい。
・ Uracil-N-Glycosylase によるキャリーオーバー処理は出来ません。

prophylactic purposes; (b) testing, analysis or screening services, orinformation in return for compensation on a per-test basis; or (c)manufacturing or quality assurance or quality control, and/or(2)

sell or transfer this product or its components for resale, whether or
not resold for use in research.

For information on purchasing alicense to this product for purposes other than as described above,
contact Life Technologies Corporation, 5781 Van Allen Way,Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
本製品は、試薬（試験研究用）として販売しているものです。
医薬品の用途には使用しないでください。

株式会社ニッポンジーン

TEL 076-451-6548

FAX 076-451-6547

www.nippongene.com
HO1808-KM2309