Topographic Raman Imaging

TrueSurface Microscopy™

Chemical Analysis and Profilometry in One Pass

Topographic Raman microscopy image of microstructured
silicon with impurities (maximum height variation 8 µm).

https://raman.oxinst.com

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TrueSurface Micros
Topographic Raman Imaging

TrueSurface is a patented optical profilometry technology pioneered byWITec. It allows confocal Raman microscopy to be applied to roughly
textured and very tilted samples by keeping their surfaces in constant focus.
This technique is known as topographic Raman imaging.
Why use TrueSurface? To achieve the highest resolution and signal-to-noise ratio
in confocal microscopy, it is essential to keep the sample surface precisely in focus.
Measuring rough or inclined samples can thus be challenging. To overcome thisobstacle, WITec developed TrueSurface technology. It actively compensates forheight variations throughout the entire measurement, resulting in perfectly sharp
and detailed images.

Working Principle
A sensor actively monitors and maintains a set distance
between the objective and sample surface with submicrometer resolution using closed-loop feedback
operation. Thus, Raman imaging can be carried out
precisely along or at a set distance from irregularsurfaces. Topographic and chemical information are
obtained at the same time.

DDD

D = Set distance
The TrueSurface™ microscopy option enables theprecise tracing of the surface while simultaneously
acquiring Raman imaging data, resulting in a
topographic Raman image.

Benefits
One-pass simultaneous optical profilometry and Raman imaging
Sharp 3D chemical imaging on coarsely textured or inclined samples• No specialized sample preparation necessary• Closed-loop feedback to compensate for dimensional variations
during long measurements••

D = Set distancecopy

TrueSurface microscopy in the arts and archaeology

Topographic Raman image of a piece of an ancient wall painting.
The maximum height difference within the investigated area was 500 µm.
Sample courtesy of Prof. Dr. Admir Masic, Department of Civil and Environmental Engineering,MIT, Cambridge, MA, USAB

C

acetaminophen

Intensity (a.u.)

aaspirin
excipient 1
caffeine
excipient 2600

1200

1800

2400

Raman shift (cm-1)

Topographic Raman imaging of a pharmaceutical tablet(A) Topography of a painkiller tablet (scan range: 7 x 7 mm²). (B) Topographic Raman microscopy
image of the same sample area (overlay of topography and Raman image). (C) Raman spectra of
the tablet’s chemical components.

Award-Winning Technology
Topographic Raman
microscopy image ofcellulose fibers in paper.

3000

alpha300 S:
Scanning Near-field
Optical Microscopealpha300 A:
Atomic ForceMicroscopealpha300 R:
Confocal Raman
Microscopealpha300 apyron™: AutomatedConfocal Raman Microscope

We take care – WITec uses environmentally friendly printed materials.
While this policy is only a small contribution to a healthy environment, we at
WITec believe that focusing on details can effect positive change in the world.

WITec Microscopesalpha300 Ri:
Inverted Confocal
Raman Microscope

RISE®: Raman Imagingand Scanning Electron
Microscopealpha300 access:Confocal Micro-Raman System

Find your regional WITec and Oxford Instruments
contact at https://raman.oxinst.com/contact
WITec Headquarters
WITec Wissenschaftliche Instrumente und Technologie GmbH
Lise-Meitner-Str. 6, D-89081 Ulm, Germany
Phone +49 (0) 731 140 700, Fax +49 (0) 731 14070 200
info@witec.de, https://raman.oxinst.com

Explore the TrueSurface
product pagehttps://raman.oxinst.com/products/
imaging-options/truesurface