Optogenetics catalog 7.5 -2

Optogenetics catalog 7.5 - Optogenetics products catalog overview

3

Table of contents
Optogenetics products catalog overview...........................4
Products road map............................................................ 7
Fiber-coupled light sources............................................... 9
Splitters/Combiners with fiber-optic connectors...............25
NA converter.................................................................... 31
Connectorized U-Bracket................................................ 33
Fiber-optic Rotary Joints.................................................. 34
Electrical rotary joint........................................................ 43
Hybrid Rotary Joints (optical, liquid and electrical)..........45
Fiber-optic cables for optogenetics .................................49
Fiber-optic Cannula......................................................... 58
Stereotaxic holders and adapters....................................75
Accessories..................................................................... 78
Optogenetics kits............................................................. 83
Single-cell fiber-optrode cannula kit................................88

Optogenetics catalog 7.5 - Optogenetics products catalog overview

4

Optogenetics products catalog overview
Optogenetics is a scientific method, as a matter of fact, the Method of
the Year 2010 according to Nature Methods magazine, that enables
control of targeted cell functions using light stimulation and geneticallyencoded light-sensitive proteins. Currently, the most popular method of
optical stimulation in optogenetics research is to send the light, from alaser or an LED source, along an optical fiber to the brain of a laboratory
animal, typically mouse or rat. As time goes by, this simple optical link isevolving into more complex circuitry, resembling an early fiber-optic
telecommunication network. This fiber-to-the-brain (FTTB) network
consists of fiber coupled light sources and their drivers, light shutters or
modulators, rotary joints for in vivo experiments, beam-splitters, fiber-optic
patch cords, variety of fiber-optic cannulas with implantable fibers and thelist goes on. And that network is not using only optical signals. It is actuallya hybrid network monitoring interaction of tissue with light, electricalsignals and different fluids. The applications where the optical fiber is used
to illuminate neurons, cells and other tissues and to collect laser-induced
fluorescence, back-scatter etc. are becoming common. The use of the
optogenetics method is no more limited to neurons and is being applied toother cell types, e.g., heart muscle cells.
Due to an ever growing demand for new products and customization,
we have outlined the optogenetics product road map that states what isbeing produced, what we anticipate that customers might need and where
we are focusing our R&D efforts. We are trying hard to educate ourselves
about those needs and we do invite you, our customer, to suggest or
discuss with us further expansions of that road map.

Optogenetics catalog 7.5 - Optogenetics products catalog overview

5

OTPG4

LEDRV_1CH

LEDRV_2CH

LEDRV_1CH

LEDC1
LEDC2

LEDFRJ

FRJ_FC-FC
FRJ_1x2_FC

MFC
MFC
TFC

Optogenetics catalog 7.5 - Optogenetics products catalog overview

6

OTPG4

Infusion
Electrical
recording
LEDRV_1CH

LEDFL_1CH

FRJ_FC-FC

HRJ-OL

ERJ

OFC
MFC

Electrodes

Optogenetics catalog 7.5 - Products road map

7

Products road map
Optogenetics TTL Pulse generators
TTL

4ch

8ch

Light sources
LED Light sources

SingleDual

TripleQuadruple

Driver





N/A



8 LEDsConnectorized modules









LED Light-sources











LED+Fiber-optic cannula





N/A

N/A

N/A

LED + Rotary Joint









Connectorized splitters/combiners
Intensity
division

Wavelength division

Fluorescence

Doric Mini Cubes







Multiple intensity
splitters



N/A

N/A

Multiple spectral splitters

N/A





Rotary joints
Fiber-optic Rotary
jointsIntensity
division

Wavelength
division/combining

Fluorescence/Imaging

1x1 passive

N/A

N/A

F

1x1 high RPM

N/A

N/A

F/I

1x2





N/A

2x1

N/A

Inverted 1x2 



1x4





N/A

4x1

N/A

Inverted 1x4 

2x2

Wavelenth combination +Intensity splitting

nxnElectrical Rotary joints
passive

6 channels

12 channels

Hybrid Rotary Joint OL(optic, liquid)

Optic 1x1, Fluid 1 to 6 (22,25 ga)

Optic 1x2 wavelength
or intensity splitting,fluid 1 to 6 (20-25 ga)

Optogenetics catalog 7.5 - Products road map

8Patch cords
Fiber-optic Patch
cords

Mono fiber

Dual fiber

Branching fiber patch
cord







Dual fiber

Two ferrule

CannulasFiber-optic cannula

Mono fiber

Ferrule 1.25

Ferrule 2.5



M3



M2



Rectangular MagneticHybrid Cannulas

Optical & fluid

Optical & electrical

Optical, electrical &
fluid

M3





development

Rectangular Magneticdevelopment

Stereotaxic tools
Stereotaxic tools

Holder
1.25mmFerrule 1.25

Ferrule 2.5

Holder 2.5mm

M3 adapter

M2 adapterMagnetic receptacleOptogenetics catalog 7.5 - Optogenetics TTL Pulse generator

9

Optogenetics TTL Pulse generator
The optogenetics methods use lightpulses to modulate the activity ofgenetically engineered light sensitive
cells like neurons or others. Long
gone are the days when sending of acontinuous
streakof blue lightalong the optical fiber to the micebrain would make him running andprovokeworldwide
scientificsensation.
Today’s
optogenetics Optogenetics TTL Pulse Generator
4 channels
experiments require light pulse trainsthat correlate not only to muscle
contraction but to number of other tissue events, monitoring andmeasurements. LEDs and laser diodes are the light sources well-suited tomodulation by TTL pulse generators. In order to facilitate vertical
integration of our optogenetics hardware, we have developed TTL pulse
generator that seamlessly integrates with the rest of our optognetics
product portfolio. The pulse train parameters and its triggering are
specified via open source software and its graphical interface. The pulse
generator needs to be connected to computer with USB cable and with
BNC cable to Doric or any other LED driver. Currently the pulse amplitudeis fixed at 5V. However, the next generation of Doric LED drivers,
expected later in the year, will allow for manual amplitude setting.
Four channel TTL pulse generator can drive up to four LED drivers or LED
sources. However, this number is if some of the channels are used as
triggers. Example: Four channels pulse generator can control pulsing of
two LED sources with Channel 1 and Channel 2 while Channels 3 and 4
are used as triggers.
The pulse programming is done using freeware that can be downloaded
from our website.

ORDERING CODE: OTPG_□
Number of channels: 4 or 8
Related products: LED drivers 1 to 4, Branching Fiber Patch cords (1xN)
and LED fiber-optic light sources 1 to 8 channels.

Optogenetics catalog 7.5 - Fiber-coupled light sources

10

Fiber-coupled light sources
The fiber optic circuits are driven by light and hence the need to couple
the light sources into the optical fiber. The most common sources used tocouple the light into the fibers are the laser diodes and LEDs, while theminiature incandescent lamps are used only when broad and smooth
spectrum is needed. The incandescent lamps can be used as primarysources from which different spectral bands and profiles can be extracted.
The light sources like LEDs or laser diode require specific drivers
that are either integrated within the light source or they are made and soldseparately. We offer stand alone LED drivers for one or several diodes, orconnectorized LEDs packages for up to 4 different wavelength LEDs andthe fiber-optic light-sources that integrate up to four connectorized LED(s)with their respective drivers within one box.
Our drivers can be used within wide range of inputs, 100–240 VAC, 5060 Hz. The power cord terminations or plugs toward wall socket are
selected to match the destination country standard.

LED drivers
Depending of the number of channels, our LED drivers support manual
CW operation of up to four independent high brightness LED modules. For
each driver’s channel, there is a BNC connector that allows independent
TTL or analog modulation of respective LED with an input amplitude from0 to 5V and the frequency of up to 10kHz. Another BCN connector gives
feedback of the LED signal with an oscilloscope. To avoid potential LED
damage, the maximum current is factory limited to 1A or to customer
selected maximum value. When drivers are used for implantable LEDs the
maximum current is limited to 0.35 A or 0.5 A.

ORDERING CODE: LEDRV_□CH_□□□□
Number of channels 1, 2 or 4
Imax (mA) 350, 500, 1000
For each LED channel, there is a M8-4pin female receptacle on the front
panel of the LED driver. The electrical connection to our LEDs is via 1.5
meter long cable with corresponding M8-4pin male and female
connectors.

Optogenetics catalog 7.5 - Fiber-coupled light sources11

Two channel LED driver

Two channel LED driver powers-up one or two single LED modules of ourdual connectorized high brightness LED module. To put it simply, thisdriver is like two single channel drivers in one box.

The four channel LED driverprovides tunable DC current tofour individual LEDs or clustersof two, three or four diodes
combined into single optical
output. The 4 channel LED driveris more silent and more compact
by volume per channel than its 1
and 2 channel brethren.
Four channel LED driverOptogenetics catalog 7.5 - Fiber-coupled light sources

12

Connectorized single LED
Our robust and compact modules
couple high brightness LEDs into different
types of optical fibers via FC receptaclethat allows change or replacement of thefiber-optic patch cord. The novelty is an
optimized heat sink design with active
cooling that enhances LED’s longevity as
well as thermal and spectral stability.
Our micro-optic approach provides
maximum coupling possible efficiency
and is well-suited for coupling LEDs into
multimode optical fibers.The coupling Connectorized High brightness
single LED
efficiency depends on LED emitter size,
fiber core diameter and fiber’s numericalaperture. The factory testing of the optical coupling is with 1 mm plastic
optical fiber. Refer to Table 1 for detailed LED specifications.
The module features M8-4pin male connector. The electrical connection to
our LED driver is via 1.5 meter long cable with corresponding M8-4pin
male and female connectors normally provided with the driver.

ORDERING CODE: LEDC1-B_FC
LED Color code
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources

13

Connectorized dual LEDs
This package combines the light from two LEDs of different color into asingle output fiber and uses patent pending regular pentagon mirrors
configuration. The coupling efficiency for respective colors is nearing thoseof our single LED sources. Refer to Table 1 for details. The LEDs are
driven independently from each other via two M8 connectors whenconnected to any of our drivers like two Single channel LED drivers, Two
Channel LED driver or Four channel LED driver. The electrical connection
to our LED drivers is via two 1.5 meter long cables with corresponding M84pin connectors.

ORDERING CODE : LEDC2-B/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources

14

Connectorized 3 LED clusters
Using three LEDs of different emissions spectrum coupled to the same
optical fiber adds new light firing capabilities to Optogenetics toolbox. The
package uses compact and efficient patent pending regular pentagon
mirror configuration. The LEDs are driven independently from each othervia our Four Channel LED driver.

ORDERING CODE : LEDC3-B/G/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources

Connectorized 4 LED clusters
Using four spectrally different LEDs coupled to the same optical fiber
further enhances light firing capabilities to Optogenetics toolbox. The
package uses compact and efficient patent pending regular pentagon
mirror configuration.

ORDERING CODE : LEDC4-U/B/G/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

15

Optogenetics catalog 7.5 - Fiber-coupled light sources

16

LED Fiber-optic Light-sources
To operate pigtailed or connectorized LEDs, one needs appropriate
drivers. We offer fiber-ready LEDs and LED drivers as separate packages
that need to be connected with electrical cables. However, for those who
prefer plug and play approach, we have introduced the fiber-optic LED
light-sources featuring the driver and connectorized LED within a samebox. While preserving all functionalities of the LED drivers andconnectorized LEDs, our fiber-optic LED sources offer simplicity of one
box design, improved thermal management, patented coupling assemblyand more informative and user-friendly interface.

Single LED fiber-optic light-source
When only one colorLEDsourceisneeded, this union ofLEDdriverandconnectorized LED is
the least expensive
and most compact
option that provides
full control over CW
operationandmodulation of thelight signal.

Single LED fiber-optic light-source

ORDERING CODE: LEDFL_B_FC
LED Color code
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources

Dual LED fiber-optic light-source
When two LEDs ofdifferent colors need to becoupled into one fiber, one canuse two channel LED driverand connectorized dual LED
or this Dual LED fiber-optic
source which, as a single
package,providesunsurpassed
simplicity,
patented coupling assembly,
low cost and space saving
option.

LEDFL2ORDERING CODE: LEDFL_B/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

17

Optogenetics catalog 7.5 - Fiber-coupled light sources

18

4 LED fiber-optic light-sourceThis is our top of the line light source that provides unprecedented light
coupling efficiency based on our patented regular pentagon assembly of
dichroic filters. Each LED has independent current modulation and canwork in analog, digital and manual mode. The output of all LEDs is
combined into one fiber connector.

ORDERING CODE: LEDFL_U/B/G/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Four channel LED fiber-optic light source

Optogenetics catalog 7.5 - Fiber-coupled light sources

Multiple fiber-optic light sources
This product consists of 2 to 8
independent LED light sources,
each with its own fiber-optic
connector, CW amplitude knob
and TTL modulation port.
The device can be used with awide range input voltage
100-240 (V) AC, 50-60 Hz.

8 blue LED fiber-optic light-source

ORDERING CODE: LEDFL_2A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

ORDERING CODE: LEDFL_8B_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Application: The product can be used to illuminate fiber cannula
arrays up to 1x8

19Optogenetics catalog 7.5 - Fiber-coupled light sources

20

Single LED + Fiber-optic cannula
We have developed an assembly where the LED is
the integral part of the fiber-optic cannula thus
providing an lightweight optical source attached tothe head of the animal suitable for deep brain
illumination. The protruding optical fiber is implanted
into the skull.
In order to keep the assembly small and lightthere is no heatsink. To limit the heat generation,
only medium brightness LEDs are used. Maximum
current should be limited to 150mA.
The electrical connector is so called Header with
3 positions 0.050” gold wire Male Pins (Sullins part #GRPB031VWVN-RC).

LED + Fiber-optic
cannulaORDERING CODE : LFC-B_□□□□□-□□□_□□□
LED color code
B or A are stock items
Other colors from Table 1 available ascustom productFiber-optic code
200-0.53 for glass fiber 200 um core - NA=0.53
240-0.63 for plastic fiber 240 um core - NA=0.63
Fiber length “L” (mm)

Accessories
Due to the lack of standard use of those implants, we provide 3 wires 15
cm long (or longer if needed) electrical cable with stripped wires on an end
and Female socket connector header (Sullins part # LPPB032CFFN-RC)
on the other end.

Optogenetics catalog 7.5 - Fiber-coupled light sources

21

LEDs + FRJ
It is a common practice to connect LED
light sources or connectorized LEDs with rotary
joints via fiber-optic patch cord. If that patch
cord has uncoated fiber ends, which is often thecase due to the extra cost and complexity, atleast 8% of the light is lost to Fresnel reflection,
not to mention other possible connection losses.
One way of getting around this avoidable loss is
to integrate the LED or LEDs within the fiberoptic rotary joint, thus eliminating that fiber patch
cord all-together. That is exactly what we did byjoining our legendary fiber-optic rotary joint andequally well known LED sources.

LED+FRJ

The surface of LED emitters is typically larger than the diameter of the
optical fibers commonly used in optogenetics experiments and that leads
to more uniform optical coupling. All our LED assemblies with integrated
rotary joints have M8 connectors for each LED in the package.

Single Led + Fiber-optic Rotary Joint
ORDERING CODE: LEDFRJ-B_FC
LED Color code
B or A are stock items
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources22

Dual Led + Fiber-optic Rotary Joint
This patent pending assembly is perfect for light activation of
Channelrhodopsin-2 (ChR2) with 473 nm blue light and Halorhodopsin
inhibition with 589 nm yellow light. However, other LED wavelength
combinations are also possible as long as they are not overlapping.

ORDERING CODE: LEDFRJ-B/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources23

3 Leds + Fiber-optic Rotary Joint
Combining three different colors is very old problem and has been triedin numerous ways when synthesizing the “white light” or making highquality color cameras. With new type of opsins emerging on a daily bases,
it is very likely that a new wavelengths will be needed within standard
activation set. This patent pending assembly provides possibility to
combine three distinct wavelengths and couple them to rotating opticalfiber.

ORDERING CODE: LEDFRJ-B/G/A_FC
LED Color Codes
Other colors from Table 1 available ascustom product
Receptacle code
FC is stock item, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-coupled light sources

4 LEDs + Fiber-optic Rotary Joint
Using four spectrally different LEDs coupled to the same optical fiber
further enhances light firing capabilities to Optogenetics toolbox. The
package uses compact and efficient patent pending regular pentagon
mirror configuration.

ORDERING CODE: LEDFRJ-U_B_G_A_FC
LED Color Codes
Other colors from Table 1 available ascustom product

Receptacle code
FC is standard, SMA available as custom product.

24

Optogenetics catalog 7.5 - Fiber-coupled light sources25

LED specifications
Table 1: LED color code and main specifications
LED

FIBER OUTPUT POWER @1000mA

Color

Code

Wavelength

Bandwidth
FWHM

Near UV

385

385 nm

15 nm

core 200µm
NA = 0.48

core 240µm
NA = 0.63

core 400µm
NA = 0.48

core 960µm
NA = 0.50

2.0 mW

2.2 mW

8.0 mW

25 mW

U

405nm

-

-

-

-

-

Royal

Y

455 nm25 nm

3.0 mW

3.5 mW

16 mW

65 mW

Blue

B

465 nm25 nm

5.0 mW

5.6 mW

22 mW

80 mW

Cyan

C

505 nm30 nm

2.0 mW

2.2 mW

8.0 mW

25 mW

Green

G

515 nm35 nm

2.0 mW

2.2 mW

8.0 mW

25 mW

Amber

A

595 nm20 nm

0.8 mW

1.0 mW

3.0 mW

12 mW

OrangeH

625 nm20 nm

3.5 mW

3.5 mW

13 mW

35 mW

Red

R

635 nm20 nm

3.5 mW

4.0 mW

16 mW

40 mW

Infrared

850

850 nm45 nm

3.0 mW

3.0 mW

16 mW

20 mW

Infrared

940

940 nm35 nm

2.0 mW

2.0 mW

9.0 mW

20 mW

White

W45

4500 K

250 nm

2.5 mW

3.0 mW

8.0 mW

35 mW

White

W55

5500 K

250 nm

3.5 mW

4.0 mW

12 mW

55 mW

Optogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

26

Splitters/Combiners with fiber-optic connectors
As fiber optics is finding wider use in microscopy, optogenetics and life
sciences in general, it is becoming increasingly popular to use wavelengthand intensity division or combination of the light within fiber optic circuits.
The beam-splitters have been used in optics for many years and almost
exclusively within the parallel beam of light and at 45 degrees angle ofincidence. Since the light coming out of the optical fiber is divergent, itneeds to be made parallel or collimated before the beam-splitters can beused. For combining or splitting of the light output from optical fibers
requires good collimation lenses, beam-splitters with steep transition
curves and precision positioning to get efficient coupling. Inspired by
microscopy cubes and the need for user friendly beam-splitting in thefiber-optics applications, we have developed a family of doric mini cubes
and multiple splitters, that integrate beam-splitting glass plates, collimationlenses and fiber-optic receptacles, all insmall and convenientconnectorized packages. Apart from shrinking the size of the cubes wehave introduced higly efficient beam-splitters with unprecedented balanceof the s and p polarization reflection curve based on our patent pending
low angle of incidence design.

Doric Mini Cubes: Intensity divisionThis mini cube contains a beam splitter that
separates a beam in two output beams with equal
power. This cube can be effectively used only as asplitter.
The receptacles can be chosen between FC,
SMA or M3. The output receptacles are typically of
the same type but can be different if needed.

ORDERING CODE: DMC_1x2i_VIS_FC
VIS for 450 to 650 nm
Other ranges available as custom product
Input receptacle code

Doric Mini cube
Intensity DivisionOptogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

27

FC is standard, SMA available as custom product.
The angle of incidence of light to partially dichroic mirror inside standard,
intensity division Doric mini cubes is 22.5 degrees that helps better control
s and p polarization.

Doric Mini Cubes: Wavelength division
The wavelength division mini cube
contains a dichroic mirror that combinesor separates different wavelengths.
Consequently, the cube can be used asa splitter, a combiner or to separate
excitation
andfluorescencewavelengths. This version of mini cube
has no other filters except a dichroic
mirror. However, the sensitivity offluorescence measurements of thedoric mini cube can be increased if
narrow band filters for excitation andfluorescent channel are added.
Externally, this is done by adding
Connectorized U-Brackets with desired
filters to respective channels. If the Doric Mini cube for separation of
470nm and 590nm - standard
specific filters are put inside the cube, itversionbecomes Fluorescence Mini Cube.

ORDERING CODE: DMC_1x2w_470/590_FC
Wavelength 1 (nm)Wavelength 2 (nm)Receptacle code
FC is standard, SMA available as custom product.

Optogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

Beam combiner

Beam splitter

28

Separation of excitationand fluorescence beams

Doric Mini Cube – Wavelength division

The angle of incidence oflight to the dichroic mirror
found inside standard Doricmini cubes is 22.5 degrees.
That helps better control of sand p polarization. For
customers using traditional
dichroic filters made for 45
degrees angle of incidence,
we can make use of them ina custom product as shownon this picture. Call us fordetails.

Example of custom assembly Doric mini cube for separation of 470 nm
and 530 nm band

Optogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

Fluorescence Mini CubeFor
more
precisefluorescencemeasurements we have devised a cubethat incorporates dichroic splitter, thenarrow band pass filters for excitation
light and corresponding filter for
fluorescent spectrum, all within the same
cube. The LED sources typically havebroader spectrum and filtering the input
with narrow band filter is highly
recommended. The same applies tofluorescence detection channel where theappropriate long pass filter greatly
improves signal to noise ratio.

Fluorescence mini cube

ORDERING CODE: FMC_1x2f_□□□/□□□-□□□_FC
Excitation Wavelength (nm)Fluorescent Wavelength Range (nm)Minimum – Maximum
Receptacle code
FC is standard, SMA available as custom product.

29

Optogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

30Light Intensity Distributor
The fiber coupled laser sources typically offer
high intensity within relatively small fiber
diameter. When running several simultaneousin-vivo experiments with those types ofsources, it makes perfect sense to use thelight distributor which is basically an intensity
splitter. By doing this, the required number ofmodulation channels, drivers and opticalsources can be reduced. Our patent pending
Light intensity distributor provides compact,
connectorized package with low insertion andpolarization dependent loss (PDL), ideal for
multimode fibers.

Light intensity distributor

ORDERING CODE: LID_1x4_VIS_FC
or
LID_1x3_VIS_FC
Receptacle code
FC is standard, SMA available as custom product.
VIS stands for visible wavelength range from 450 to 650 nm. Other ranges
available as custom product. The expected intensity percentage in eachchannel is typically slightly less than 100 divided by the number ofchannels.
NB Our standard products assume the use of identical fiber diameters,
receptacles and equal intensity for each channel. However, this can becustomized if needed at extra cost.
The main difference between Doric Mini Cube and Light Intensity
Distributor is the angle of incidence to dichroic mirror which is in the latercase 18 degrees. The small angle of reference minimizes differences
between the s and p polarization and improves the compactness of theassembly.

Optogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

31

Light Spectrum Mixer
For in-vivo optogenetics experiments there isa need to illuminate the tissue with specificpulses of spectrally different light using thesame fiber. To put it simply, the light fromdifferent fiber coupled LEDs or lasers needs tobe combined into one beam and coupled to an
optical fiber leading to fiber-optic implant orcannula. Our patent pending light spectrum
mixer provides compact, connectorizedpackage with highly efficient coupling and low
polarization dependent loss (PDL), ideal for
multimode fibers.

Light spectrum mixer

The same device can be used in the opposite direction as a light spectrum
separator. The concept of spectrum mixer or splitter is analog to conceptof wavelength division multiplexing and demultiplexing
in opticaltelecommunication.

Optogenetics catalog 7.5 - Splitters/Combiners with fiber-optic connectors

32

ORDERING CODE: LSM_1x4_405/470/530/590_FC
or
LSM_1x3_
470/530/590_FC
Center wavelengths (nm)Other LED or laser wavelengths available as custom product
Receptacle code
FC is standard, SMA available as custom product.
NB Our standard products assume the use of identical fiber diameters andidentical receptacle codes. However, this can be customized if needed at
extra cost.
The main difference between Doric Mini Cube and Light Spectrum Mixer isthe angle of incidence to dichroic mirror which is in the later case 18
degrees. The small angle of reference minimizes differences between thes and p polarization and improves the compactness of the assembly.

Optogenetics catalog 7.5 - NA converter

33

NA converter
The Lagrange invariant can be defined as a
product of numerical aperture and diameter of the
optical fiber. With a lens system that re-images
the light from one fiber end to the other, one canchange the light cone converging toward theother fiber (NA) and create different spot size.
NA converter
However, the product of NA and spot diameter or
Lagrange invariant remains the same as its name implies.
When coupling multimode fibers with different diameter and/or
numerical aperture (NA) the following situations can be encountered:-Input fiber with smaller diameter and smaller NA couples well with output
optical fiber with larger diameter and NA. Common connectors like FC/PC,
SMA and others can be used. However, in the opposite direction theconnection is very lossy.
-The coupling between optical fibers ofsimilar values of Lagrange invariant but
different NA and core diameters cannot be
butt-coupledwithout
introducingasignificant loss in both directions.
Lagrange invariant - 2X
However, their coupling can be effectively
magnification
optimized using a lens system with theappropriate magnification. Then, the connection has a high transmission inboth directions. For the lab situations where different types of fibers haveto be connected, these NA converters can be effective match makers.
They can also be used to increase the filling of the fiber's NA resulting inlarger illuminating cone on the other end of the fiber.
Example:If input fiber has 0.48 NA and 100 um core diameter, the lens systemwith 2X magnification will reduce the NA on the other side of converter to0.24 and increase the spot size to 200 um.

Optogenetics catalog 7.5 - NA converter

ORDERING CODE: NAC_□□□_□□□_FC
Input NA
Output NA

Receptacle code
FC is standard, SMA available as custom product.
Output NA equals Input NA divided by Magnification.

34

Optogenetics catalog 7.5 - Connectorized U-Bracket

35

Connectorized U-Bracket
Sometimes it is necessary to attenuate the lightsignal or to filter its spectrum within a fiber-optic
link. A simple connectorized U-bracket allows
setting the desired intensity attenuation or spectral
filtering by inserting the appropriate mini filter fromthe mini filter set. To state the obvious, it isnecessary that NAs and diameters of input and Connectorized U-bracket
output fibers are of the same or similar typeand filter insert
unless some loss is tolerated. The NA of the fibers
should be less than the NA of the collimating lenses.

ORDERING CODE: CUB_0.5_FC
Max fiber NA
Receptacle type
FC is standard, SMA available as custom product.

U-Bracket Inserts
The inserts can be fitted with attenuating filters orspectral filters made from a variety of glass materials. Asa matter of fact, any filter can be fitted to our standard
filter holder and its characteristics or code can beengraved on its body. In this way you can build your set
Attenuating or
using off the shelf and custom filters.
spectral filter

insert
The narrow band filters can be useful for filtering
fluorescence excitation spectrum or for the fluorescence light.

ORDERING CODE: UBI_□□□□□_□□□□□
Glass type or Filter glass manufacturere.g. Semrock, Omega, Chroma, Schott
Manufacturer part number
or Attenuation (% or dB)
Examples: UBI_Semrock_FF01-474/23-25,
UBI_Chroma_ET470/40x

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

36

Fiber-optic Rotary Joints
The fiber-optic rotary joint optically connects the fiber-optic tips within
the fiber receptacle on the fixed side and on the rotating side of the joint. It
consists of high precision bearings and a lens system which allow arotation-insensitive optical power transfer between the fiber tips. In some
optogenetics experiments, the optical fiber is connected to the mousehead and when the mouse moves inside confined space, the rotary joint
releases the twisting of the optical fiber. The nomenclature that we use forthis product is FRJ_mXn where m represents the number of input fiberson the fixed side and n represents the number of output fibers on therotating side.

1x1 Fiber-optic Rotary Joint
Basic, most popular type of the rotary joint. It
consists of a body, two bearings, two collimating
lenses and of a receptacle on each side. Whenconnectors are inserted in receptacles the fiber tips
are in the focal planes of the respective collimating
lenses. Between the lenses the beam is parallel.
Typically used with optical fibers with core diameterof 200µm and NA of up to 0.5.
1x1 fiber-optic rotary
joint FC output

ORDERING CODE: FRJ_1x1_FC-FC
Intput Receptacle CodeFC is stock item, SMA
available as custom productOutput Receptacle CodeFC and M3 are stock items, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

37

1x2 Fiber-optic Rotary Joints
1x2 fiber-optic rotary joints have a single fiber receptacle on the fixedside and two fiber receptacles on the rotating side. These rotary joints areused to send light coming from a single optical fiber to two points on themoving target via separate optical fibers.
There are two distinct versions of this product, one for the intensity
division and the other for the wavelength division of light. Each version
can be further customized if needed.

Intensity division
The intensity division rotary joints send half of the input light into eachof the two output receptacles. The input receptacle is typically the FC typewhile output receptacles can be any of M3, SMA or FC types.
The rotary joint with FC receptacle on
input side and M3 receptacles on therotating side is small and compact andmeets the low rotation torque requirementsof some optogenetics experiments. The
rotary joint with FC or SMA output
connectors is somewhat larger. The fiber
patch cords with corresponding connectors
should be used to connect to rotary joints.
The other side of those patch cords can be
fitted with the M3 connectors, magnetic
connectors or with a fiber ferrule that 1x2 fiber-optic intensity
connect to fiber-optic cannula. For morerotary joint
information on the matching patch cords
for rotary joints, see the patch cord
section.

ORDERING CODE: FRJ_1x2i_FC-2FC_□□□
Input Receptacle CodeFC is standard, SMA available as custom product.
Output Receptacle Code (same for both fibers)
FC and M3 are stock items, SMA available as custom
product.
Numerical Aperture
0.22 or 0.5

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

38

Wavelength division
The wavelength division rotary joints split the spectral band originatingfrom the input receptacle and send each band to the corresponding
rotating fiber receptacles. In some optogenetics experiments, they can beused for example to separate the 473-488 nm blue light (ON signal) andthe 590 nm orange light (OFF signal).
The rotary joint with FC receptacle on input
side and M3 receptacles on the rotating side issmall and compact and meets the low rotation
torque requirements of some optogenetics
experiments. The rotary joint with FC output
receptacles is somewhat larger. The fiber
patch cords with corresponding connectors
should be used to connect to rotary joints. On
the output side, the loose ends of the fibers
can be terminated with the M3 connectors,
magnetic connectors or with a fiber ferrule thatconnect to the fiber-optic cannula. For more
information on the matching patch cords forrotary joints, see patch cord section.

1x2 fiber-optic rotary joint
wavelength division

ORDERING CODE:
FRJ_1x2w_473/590_FC-2FC_□□□
Wavelengths (nm)
Input Receptacle CodeFC is stock item, SMA and M3 available as custom productOutput Receptacle Code (same for both fibers)
FC and M3 are stock items, SMA available as custom product.
Numerical Aperture
0.22 or 0.5

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

39

Interestingly enough, the wavelength division
1x2 rotary joint can be used in the opposite
direction as spectral combiner. In optogenetics
stimulation applications that means combining
activation and silencing fiber coupled lightsources on a static part of the rotary joint anddelivering those beams to single rotating fiber on
the other side of the joint.

2x1 fiber-optic rotary joint
combines two different
wavelengths

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

40

Shared Light Path 2x2 FRJ
The simplest form of 2x2 rotary joint isused to mix two spectrally different Under construction
sources on the fixed side and to do
intensity split on the rotating side. This is 4x1 fiber-optic rotary joint intensity
division with FC outputs
anexcellent
tool
forbilateral
optogenetics stimulation, be it for
activation or silencing. This tool isdesigned for people wanting to combine twolaser sources or one laser and one LED
source. Those wanting to combine two LEDsof different colors are better served with Dual
LED + FRJ or LEDFRJ-B/A_FC and
Branching patchcord.
2wx2i fiber-optic rotary joint

ORDERING CODE: FRJ_2wx2i_473/590_2FC-2FC
Wavelengths (nm)
Input Receptacle CodeFC is stock item, SMA available as custom productOutput Receptacle Code (same for both fibers)
FC and M3 are standard, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

41

Separate Light Path 2x2 FRJ
In some cases there is a need for rotary jointthat connects two arbitrary fiber-optic types on
the stationary side of rotary joint with theirrespective counterparts on the rotating side.
The examples of such applications areplentiful and here are some of them:- In vivo fluorescence measurements where
one path is used for excitation light while theother path is used by collecting fiber with highdiameter and high NA,- Concurrent optogenetics experiments of two 2x2 fiber-optic rotary joint
different brain centers having different
illumination modulation,- Increasing the power of LED illumination, e.g. amber, by combining two
fiber coupled LEDs over strategically positioned fiber-optic cannulas andthe list goes on.
This innovative patent pending technology offers unprecedented
possibilities for laser or LED based optogenetics lighting and otherapplications requiring compact and low loss dual channel fiber-optic rotary
joint.

ORDERING CODE: FRJ_2x2_VIS_2FC-2FC
Wavelength range VIS, IR, ...
Input Receptacle CodeFC is stock item, SMA available as custom productOutput Receptacle Code (same for both fibers)
FC and M3 are standard, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

42

1x4 Fiber-optic Rotary Joints
These joints are used to send the light coming from a single opticalfiber to 4 different points on the moving animal via separate optical fibers.
There are two distinct versions of this product, one featuring theintensity division and the other the intensity and wavelength division oflight. Each version can be further customized if needed.

Intensity division
The purely intensity division rotary joint sends one quarter of the input lightinto each of four output receptacles. Its input receptacle is typically FC or
SMA, while the output receptacles can be M3, SMA or FC types. However,
due to the torque limitations in some optogenetics experiments, westrongly recommend the use of M3 version over bulkier FC and SMA
versions.
The fiber patch cords with corresponding connectors should be
used to connect to rotary joints. On the output side, the loose ends of thefibers can be terminated with the M3 connectors, magnetic connectors orwith a fiber ferrule that connect to fiber-optic cannula. For more
information on the matching patch cords for 1x4 fiber-optic rotary joint see
patch cord section.

ORDERING CODE: FRJ_1x4i_FC-4FC
Input Receptacle Code:FC is standard, SMA available as custom productOutput Receptacle Code: (same for all four)
FC and M3 are stock items, SMA available as custom product.

Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

43

4x1 Fiber-optic Rotary Joints
4x1 fiber-optic rotary joints have four fiber receptacles on the fixed side
and one fiber receptacle on the rotating side. They are used to combine
spectrally different light beams coming from up to four optical fibers and tocouple those beams to rotating fiber on the other side of the joint.
Unlike 1x4 FRJ which has intensity division version, the 4x1 FRJ has
only the version that combines different wavelengths.
For more information on the matching patch cords for 4x1 fiber-optic
rotary joint, see patch cord section.

ORDERING CODE:
FRJ_4wx1_405/470/530/590_4FC-FC
Wavelengths (nm)Other LED or laser wavelengths
available as custom product
Input Receptacle Code (same for both fibers)
FC is standard, SMA available as custom productOutput Receptacle CodeFC is standard, SMA and M3 available as custom product.
Numbers ofter the slash represent center wavelength (nm) for thecorresponding spectral band. Other LED or laser wavelengths available ascustom product.+Optogenetics catalog 7.5 - Fiber-optic Rotary Joints

44

Technical specifications of fiber-optic rotary joints
The principal motivation behind the development of our fiber-optic
rotary joints is to provide optogenetics research labs with simple andinexpensive passive tools for connecting light sources with freely moving
laboratory animals via optical fibers. Consequently, the main parameter toconsider is the torque needed to move the joint or the resistance to therotation of the joint. Typically smaller devices will have smaller torque.
The numerical aperture of the connecting fibers can affect the overall
transmission of the joints and we recommend that appropriate fibers be
used if the best transmission results are to be obtained. Additionally, thetransmission and its variation during rotation are influenced by the fiber
diameter. The specs shown below relate to 200µm diameter fibers.
FRJ

TorqueNA

Weight ODmax

Length Transmission (T)FRJ_FC-FC

7µN.m

0.48

18g

17mm

30mm

> 80%

FRJ_1x2i/w_FC-2M3

9µN.m

0.22

22g

19mm

36mm

> 2x35% *FRJ_1x4i/w_FC-4M3

16µN.m

0.22

38g

28mm

40mm

~ 4x16% *FRJ_1x2i/w_FC-2FC

14µN.m

0.22

117g

40mm

60mm

> 2x35% *FRJ_1x4i/w_FC-4FC

30µN.m

0.22

275g

60mm

70mm

~ 4x15% ** : valid for intensity division
For all fiber-optic rotary joints, the variation of the power with theangular position of the joint is lower than 5% per channel.

Optogenetics catalog 7.5 - Electrical rotary joint

45

Electrical rotary joint
The electrical rotary joints have
been
used
for
in-vivoelectrophysiology recordings for many
years. The arrival of optogenetics
created
the
need
for
electrophysiological recordings of the
optical stimulation of the tissue. That
requires certain degree of optoelectrical hybridization of cannulas,connecting cables and rotary joints.
The electrical rotary joint with thethrough hole is optogenetics-ready if
its through hole diameter is larger
than the diameter of fiber-optic
connector on either end of the patch
cord that connects fiber-optic rotary
joint and fiber-optic cannula implant. If
this is the case, the electrical and Electrical rotary joint - 12 channels
fiber-optic rotary joints can be used intandem. With this in mind, we havedeveloped passive electrical rotary joint with low torque of 0.9 mNm and1.8 mNm for 6 and 12 electrical contacts respectively that can be usedeither purely for electrophysiology, or, when combined with fiber-optic
rotary joints, for electrophysiological recordings of optogenetically induced
events.
The joints have 7.5 mm through hole that is sufficiently large to allow
passing of M3 connector or ferrule/sleeve type connectors across.
The joint also comes equipped with accessories for attaching our standard
1x1 or 1x2 fiber-optic rotary joints.
Notes:-The fiber patch cord must have at least one end with connectors or
ferrules that are less than through hole diameter.
-The number of electrical contacts does not necessarily equals thenumber of recording channels as some of them might be used byelectronics on a head stage.

Optogenetics catalog 7.5 - Electrical rotary joint

46

ORDERING CODE: ERJ_□□_HRW-HRW# of electrical contacts:
6, 12
Fixed side connector type:
HRW for HARWIN M80-82612 or 06 standard
WIR for 30 cm long leads custom
Rotating side connector type:
HRW for HARWIN M80-82612 or 06, WIR for 30 cm long leads
Related products: 1x1 and 1x2 Fiber-optic rotary joints, Branching FiberPatch cords (1xN)

ERJ and FRJ used in tandem

Optogenetics catalog 7.5 - Hybrid Rotary Joints (optical, liquid and electrical)

47

Hybrid Rotary Joints (optical, liquid and electrical)
To better understand the flow of ions in biological tissue or within asingle cell, the scientist use electrophysiological recordings to monitor
optogenetically marked and stimulated tissue while administering various
liquids to the observation site. For in-vivo experiments, one needs the
hybrid rotary joints that combines some or all functions of the fiber-optic,
electrical and liquid rotary joints within one instrument. For us, each of thepossible combinations of these functions is a product category on its own.

Fiber-optic & Liquid rotary joint
The optogenetics method provides thepossibility to directly observe the influence ofdifferent drug solutions on cell processes. To
enable in-vivo observations of those interactions,
we have developed hybrid optical/liquid rotary
joints that allows optical connection and liquiddelivery via up to six tubes within rotating part of
the rotary joint. This joint couples to any type ofthe liquid swivels from Instech Solomon. It
incorporates a holder for liquid swivel andcorresponding interchangeable metal tubes for
insertion of plastic tubing. It is possible toexchangetubingandprevents
crosscontamination when changing liquid solutions.

Fiber-optic and liquidrotary joint

ORDERING CODE: HRJ-OL_FC-FC
Optical Channel Input Receptacle CodeFC is stock item, SMA available as custom productOptical Channel Output Receptacle CodeFC are stock items, SMA or M3 available as custom products.
NB the liquid swivel and the plastic tubing are not included.
Related products: Optic & Fluid cannula, Branching Fiber Patch cords(1xN)

Optogenetics catalog 7.5 - Hybrid Rotary Joints (optical, liquid and electrical)

Fiber-optic & Electrical rotary joint
By combining light stimulation andelectrophysiological recordings in optogenetics
experiments, one can observe correlation ofelectrical pulses with light stimulation. At thisstage, most of research is focused on combining
the comb of electrodes with a single illuminatingfibers or one fiber one electrode. To facilitate thiskind of in vivo experiments, we have developed
passive low torque hybrid rotary joints withnumber of electrical channels and one opticalchannel with FC connectors on both ends. This
product is more compact than combination ofelectrical rotary joint and 1x1 fiber-optic rotary
joint where the fiber is passed through central
hole of the electrical joint.

Fiber-optic &electricalrotary joint

ORDERING CODE:
HRJ-OE_FC-FC_□□_HRW-HRW
Optical Input Receptacle CodeFC is stock item, SMA is custom productOptical Output Receptacle CodeFC is stock item, SMA and M3 available
as custom products.
# of electrical channels
6,12
Fixed side connector type
HRW for HARWIN M80-82612 or 06 depending on # of channels
WIR for 30 cm long leads
Rotating side connector type
HRW for HARWIN M80-82612 or 06 depending on # of channels
WIR for 30 cm long leads
Related products: Branching Fiber Patch cords (1xN)

48

Optogenetics catalog 7.5 - Hybrid Rotary Joints (optical, liquid and electrical)

49

Optical, Liquid & Electrical rotary joint
ORDERING CODE:

HRJ-OLE_FC-FC_□□_HRW-HRW
Optical Input Receptacle CodeFC is stock item, SMA is custom productOptical Output Receptacle CodeFC is stock item, SMA and M3 available
as custom product# of electrical channels
6, 12
Fixed side connector type
HRW for HARWIN M80-82612 or 06,
WIR for 30 cm long leads
Rotating side connector type
HRW for HARWIN M80-82612 or 06,
WIR for 30 cm long leads

Optogenetics catalog 7.5 - Assisted Hybrid Rotary Joints

50

Assisted Hybrid Rotary Joints
So far, we spared no effort to reduce friction in our rotary joints for in-vivo
brain research involving small animals like mice and rats. However, theincreased complexity level of today’s optogenetics experiments requires
evermore optical, electrical and liquid channels to be linked to animal’s
brain and, at certain point, there is no choice but to use assisted rotation
joints.

Assisted Fiber-optic & Electrical rotary joint
By combining light stimulation andelectrophysiologicalrecordingsinoptogenetics experiments, one canobserve their correlation. At this stage,
most of research is focused on combining
single illuminating fiber with either one
electrode or comb of electrodes. Eachelectrode requires at least one slip ring,where each ring has the braking effect on
the rotary joint. Our assisted rotary joint
removes this impediment to rotation
regardless of the number of electricalchannels. The standard joint comes with
12 electrical channels and one opticalchannel and FC connectors on both ends. Assisted Fiber-optic & electricalrotary joint
This product is more compact than
combination of electrical rotary joint and
1x1 fiber-optic rotary joint where the fiber is passed through central holeof the electrical joint.
Contact us to discuss possibilities for more electrical or optical channels.

Optogenetics catalog 7.5 - Assisted Hybrid Rotary Joints

51

ORDERING CODE:
AHRJ-OE_FC-FC_12_HRW-HRW
Optical Input Receptacle CodeFC is stock item, SMA, M3 optional
Optical Output Receptacle CodeFC is stock item, SMA and M3 optional# of electrical channels
Fixed side connector type
HRW for HARWIN M80-82612
WIR for 30 cm long leads
Rotating side connector type
HRW for HARWIN M80-82612
WIR for 30 cm long leads
Related products: Branching Fiber Patch cords (1xN)

Optogenetics catalog 7.5 - Connectorized Mechanical Shutter Adapter

52

Connectorized Mechanical Shutter Adapter
The modulation of light signal is very
importantfor
optogenetics
experiments. The light sources, like
LEDs or laser diodes are well-suited for
direct electrical modulation, while some
popular laser types require external
modulation via mechanical shutters or
acousto-optic modulators. However,mechanical shutters are gaining
popularity
with
laser
based
optogenetics set-ups as they are better
suited for use multimode fibers.
The inconvenience of mechanicalshutters is that they require parallel Connectorized Machenical Shutter
beam of light and subsequent coupling
Adapter -SRS
into optical fiber can be tricky andunstable. To facilitate the use of mechanical shutters we are providing
connectorized adapters for major shutter brands like SRS475 and Uniblitz.
These assemblies provide relatively inexpensive alternative to acoustooptic modulators. We can supply the adapter only or connectorized SR475
or Uniblitz xxx shutter head with or w/out their respective Shutter
Controllers.

ORDERING CODE: CMSA-SR475_FC-FC
Shutter type
Optical Input Receptacle CodeFC is stock item, SMA is custom productOptical Output Receptacle CodeFC is stock item, SMA and M3 available as custom products.

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

53

Fiber-optic cables for optogenetics
A fiber-optic patch cord connects two distant fiber optic ends and uses
the same type of fiber and connections as the tips of the respective fibers
it connects. In the context of optogenetics experiments with the rotary
joint, a fiber optic patch cord is needed to connect the light source and therotary joint and yet another patch cord to connect the rotary joint and thefiber-optic cannula.

Structure of a patch cord

Cladding

JacketBufferCore

The core and the cladding are two layers that make up the lightguide.
However, the light travels inside the core of the fiber-optic, barely or notinside the cladding. For this reason, interconnected fiber-optics shouldhave the same core diameter. Different cladding diameters have noinfluence on the coupling efficiency.
The buffer is a protective layer that tightly encircles the cladding. For
patch cords, we usually recommend the use of another protective layer,called jacket, which is a loose tube covering the previously mentioned
layers of the cable.

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

54

Table 2: Termination codes for patch cords
Description

PictureTerminationcode

FC connector

FC

SMAconnector

SMA

Zirconia ferrule OD=1.25mm

ZF1.25

Zirconia ferrule OD=1.25mm with flange

ZF1.25(F)Metal ferrule OD=1.25mm

MF1.25

Zirconia ferrule OD=2.5mm

ZF2.5Zirconia ferrule OD=2.5mm with flange

ZF2.5(F)

Metal ferrule OD=2.5mm

MF2.5Rectangular magnetic connector

RMC

M3 connector

CM3

M3 connector – peek plastic

CM3(P)

M2 connector

CM2

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

55

Table 3: Plastic optical fibers
Outer diameter
BufferJacketNumerical
Aperture

Fiber-optic code

250

1000

-

0.50

240/250/1000-0.50

500

1000

-

0.50

480/500/1000-0.50

960

1000

2200

-

0.50

960/1000/2200-0.50

1480

1500

3000

-

0.50

1480/1500/3000-0.50

Core

Cladding

240
480

Unless otherwise specified, the patch cords are black.
Table 4: Silica multimode optical fibers
Outer diameter
BufferJacketNumerical
Aperture

Fiber-optic code

125

250

900

0.22

50/125/900-0.22

62.5

125

250

900

0.27

62.5/125/900-0.27

100

110

125

900

0.22

100/110/900-0.22

100

110

125

900

0.37

100/110/900-0.37

100

110

500

900

0.44

100/110/900-0.44

105

125

250

900

0.22

105/125/900-0.22

200

220

240

900

0.22

200/220/900-0.22

200

240

400

900

0.22

200/240/900-0.22

200

220

245

900

0.37

200/220/900-0.37

200

230

500

900

0.37

200/230/900-0.37

200

230

500

900

0.39

200/230/900-0.39

200

230

500

900

0.48

200/230/900-0.48

200

220

500

900

0.53

200/220/900-0.53

300

330

370

900

0.22

300/330/900-0.22

300

330

650

1000

0.37

300/330/1000-0.37

400

440

480

900

0.22

400/440/900-0.22

400

430

730

1100

0.37

400/430/1100-0.37

400

430

730

1100

0.39

400/430/1100-0.39

400

430

730

1100

0.48

400/430/1100-0.48

400

440

475

900

0.53

400/440/900-0.53

Core

Cladding

50

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

56

For better fiber protection, we also offer larger jackets made of PVC
tubing. In this case, we use the following color convention, or we can use
the black jacket if preferred. Metal jacket or jackets made of othermaterials are also available on request.
Table 5: Alternative jackets for patch cords
Alternative fiber-optic codes

Jacketcolor

Fiber core
diameter (µm)

PVC jacket OD 2mm

PVC jacket OD 3mm

Red

50

50/125/2000-0.22

50/125/3000-0.22

Green

100 or 105

100/□□□/2000-□□□or
105/125/2000-0.22

100/□□□/3000-□□□or
105/125/3000-0.22

Dark blue200

200/□□□/2000-□□□200/□□□/3000-□□□Light blue300

300/□□□/2000-□□□300/□□□/3000-□□□Orange400

400/□□□/2000-□□□400/□□□/3000-□□□

Yellow

600

600/□□□/2000-□□□600/□□□/3000-□□□

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

57

Mono Fiber Patch cords
The simplest form of the patch cord is a piece of fiber with buffer
coating and two ferrules on its ends. So far, the most popular fiber in
optogenetics research is a fiber with 200 µm core diameter and NA=0.22.

Mono fiber-optic patch cord

ORDERING CODE:

MFP_□□□/□□□/□□□-□□□_□□□_□□□-□□□

Fiber-optic code
See Table 3, Table 4 and Table 5
for available codes
Fiber length (m)From ferrule tip to ferrule tip
Termination code : End 1
See Table 2 for available codes
Termination Code: End 2
See Table 2 for available codes
However, it is important to mention that diameter of the fiber and its
numerical aperture limit the useful collection angle and coupling into thefiber. Therefore, if higher coupling from sources like LEDs into optical fiberof specific diameter is needed, please select higher NA fiber and follow it
all the way through to the fiber optics cannula.

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

58

Dual Fiber Patch cords
A dual fiber patch cord has two fiber-optic strands within the jacket,
where the fiber ends are inserted into mono or dual fiber ferrules orconnectors.
Some dual fiber patch cords were especially designed to easily bring
the light from the two optical channels of the 1x2 rotary joint into a dual
fiber cannula.
There are several types of dual fiber patch cords, depending on thetype of connectors and cannulas used.

Dual ferrule

Dual fiber patch cord

ORDERING CODE:

DFP_□□□/□□□/□□□-□□□_□□□_□□□-□□□
Fiber-optic code
See Table 3 and Table 4
for available codes
Fiber length (m)(From ferrule tip to ferrule tip)
Termination code : Single connector side
See Table 6 for available codes
Termination Code: Two connectors side
See Table 2 for available codes

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

59

Table 6: Termination codes for patch cords for dual fiber patch cord
(single connector side)
Description

PictureTerminationcode

Dual ferrule with pitch 0.7mm -Titanium

DF0.7

Dual ferrule with pitch 1.0mm - Titanium

DF1.0

Dual ferrule with pitch 1.2mm - Titanium

DF1.2

Dual ferrule with pitch 1.5mm -Titanium

DF1.5

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

60

Branching Fiber Patch cords (1xN)
A branching fiber patch cord consists of multiple single fiber patch
cords that are at one end joined within one connector and its ferrule, while
the loose side of the patch cords ends with mono fiber ferrules orconnectors.
We offer fiber-optic patch cords that branch from one input to N output
connectors. These patch cords have a single FC or SMA connector on
bundled fibers end, and FC, SMA, M3, zirconia or metal ferrule for eachfiber on the other end. The optical power entering the fiber bundle isdivided between the channels.

FerruleFibersAny patch cord
termination
Connects to:»Fiber with a larger core diameter»LED source

Optogenetics catalog 7.5 - Fiber-optic cables for optogenetics

ORDERING CODE:

BFP(□)_□□□/□□□/□□□-□□□_□□□_□□□-□□□
Number of branches
Fiber-optic code
See Table 3 and Table 4 for available codes
Fiber length (cm)
(From one ferrule tip to another ferrule tip)
Termination code : Single connector side
See Table 2 for available codes
Termination Code: Multiple connectors side
See Table 2 for available codes
Example: Branching Fiber Patch Cord with two fibers
BFP_2_200/220/900_0.53_100_FC_2xZF1.25

61

Optogenetics catalog 7.5 - Fiber-optic Cannula

62

Fiber-optic Cannula
A fluid cannula is an assembly of a metal tube and a
fluid tube receptacle, used for administering fluids whenmetal tube is inserted into the body. A venous cannula
is inserted into a vein to obtain blood samples or todeliver medicines. The body of a cannula has a form
that easily connects to or disconnects from the plastic
tubing. The plastic tubing can be disconnected while
the cannula remains attached to the body surface withthe hollow needle (tube) inserted into the body for thelater use. Similar to those fluid cannulas, biomedical
and optogenetics applications need fiber-optic cannulas
to introduce the laser or LED light into the body tissue. Mono fiber-optic
cannulaAs an example, illuminating the neurons within the
Rectangularmouse brain with the blue or orange light has become
magnetic
an essential tool for studying the processes within
genetically modified photosensitive neurons. In
early days of optogenetics, researchers used a fluid
cannula to insert the optical fibers into the brain
tissue, where the metal tube was guiding the fiber
to the neurons. After the experiment, the opticalfiber was removed from the cannula only to bereinserted later. The optical fiber removal and reentry could lead to infections and clogging of thefluid cannula.
With some exceptions, the fiber-optic cannula istypically used without the metal tube of the fluid
cannula. It consists of a fiber-optic ferrule with some
sort of fiber-optic receptacle on one side and theimplantable fiber protruding from the other side.
When the fiber-optic cannula is fixed to the body
Optic & Fluid Cannula - and the fiber implanted, the light can be delivered tothe tissue and the fluorescence or scatter from theM3tissue can be captured. In these experiments, it isimperative that the connection between the delivery fiber and the cannula
is light, small and simple to connect and disconnect. For a mono fiberdelivery, the connection between the ferrules of the light delivery fiber
patch cord and the fiber-optic cannula is achieved, in its simplest form, via

Optogenetics catalog 7.5 - Fiber-optic Cannula

63

fiber-optic sleeve. The connector type connection is preferred but it is notalways applicable. In some optogenetics experiments it is necessary to
introduce two or more implantable fibers within a small, precise distance.
Those applications call for the dual fiber-optic cannula that is easily
connected to the matching delivery fibers.
The concept of fiber-optic cannulas with different optical fibers,
receptacle types and fiber terminations is bound to be further fragmented.
So far we carry Mono fiber cannulas, Dual fiber cannulas, Two ferrules
cannulas and hybrid cannulas. In effect, we are developing hybrid
cannulas that transmit combination of light, liquid and electrical signals.
Tables 7, 8 and 9 show different possibilities possible with each canula
type.
Table 7: Silica multimode optical fibers
Core Outer diameter Numerical
(µm)
(µm)
Aperture

Buffercolor

Outer Layer

Fiber-optic
code

50

70

0.22

yellow

Polymide buffer

50/70-0.22

100

125

0.22

yellow

Polymide buffer

100/125-0.22

100

125

0.37

yellow

Polymide buffer

100/125-0.37

100

150

0.44

clear

Silicone buffer

100/150-0.44

200

240

0.22

yellow

Polymide buffer

200/240-0.22

200

260

0.22

clear

Silicone buffer

200/260-0.22

200

230

0.37

clear

Hard polymer cladding

200/230-0.37

200

245

0.37

yellow

Polymide buffer

200/245-0.37

200

230

0.48

clear

Hard polymer cladding

200/230-0.48

200

245

0.53

blue

Hard polymer cladding

200/245-0.53

300

370

0.22

yellow

Polymide buffer

300/370-0.22

300

330

0.37

clear

Hard polymer cladding

300/330-0.37

400

480

0.22

yellow

Polymide buffer

400/480-0.22

400

430

0.37

clear

Hard polymer cladding

400/430-0.37

400

430

0.48

clear

Hard polymer cladding

400/430-0.48

400

475

0.53

clear

Hard polymer cladding

400/475-0.53

Optogenetics catalog 7.5 - Fiber-optic Cannula

64

Table 8: Plastic optical fibers
Core

Outer diameter

Numerical
Aperture

Buffer color

Fiber-optic code

240

250

0.63

clear

240/250-0.63

480

500

0.50

clear

480/500-0.50

Table 9: Fiber-optic termination codes for cannulas
Termination
Description
code
FLT

Axx

DrawingSpecifications

Flat tip

Angled tip

Standard angles: 45°;
60°
Other angles on request(max 60°)

θ
θ

Bxx

Bi prism tip

θCxx

Conical tip

MA45

Mirror tip
at 45°

Standard angles: 45°;
60°
Other angles on request(max 60°)
Rounded tip thickness:~ 0.1x to 0.2x core
diameterStandard angles: 45°;
60°
Other angles on request(max 60°)

Note: Axx, Bxx, Cxx and Rxx are offered to facilitate the insertion of the fiber-optic
in the tissue. However, they have little influence on the light spread.

Optogenetics catalog 7.5 - Fiber-optic Cannula

65

Mono Fiber-optic Cannula
The mono fiber-optic cannula is an assembly of a bare optical fiber, afiber ferrule and a receptacle or a sleeve. One side of the ferrule ispolished while the implantable part of the fiber protrudes from the opposite
end of the ferrule. The ferrule is placed within receptacle or sleeve toallow connecting to the fiber-optic patch cord. The protruding fiber can be
implanted into the body while the ferrule or the receptacle is attached tothe skin. When the cannula is connected with the patch cord, it is possible
to send the light signals to and from the tissue close to fiber tip. It isimperative for in-vivo optogenetics applications that the fiber-optic cannula
allows for efficient, plug and play type connection with the fiber-optic patchcord.
A receptacle is a mechanical holder that defines the positions of thefiber tip and guides the connecting ferrule to the optical coupling position.
For mono fiber-optic cannulas we offer Zirconia sleeves as the simplest
form of receptacle, M2, M3 and rectangular magnetic receptacles. For
more information refer to receptacle section.
N.B.: Zirconia sleeves are ordered separately.
The tolerance on the length of protruding fiber is better than 0.1mm.

ORDERING CODE: MFC_□□□/□□□-□□□_□□□_□□□_□□□
Fiber-optic code
See Table 7 and Table 8
Length “L” (mm)Receptacle code:
See Table 10
Fiber Termination code:
See Table 9

Optogenetics catalog 7.5 - Fiber-optic Cannula

66

Table 10: Receptacle codes for mono fiber-optic cannula
Description
Zirconia ferrule
OD 1.25mm

PictureTermination code
ZF1.25

Metal ferrule
OD 1.25mm

MF1.25

Zirconia ferrule
OD 2.5mm

ZF2.5

Metal ferrule
OD 2.5mm

MF2.5

Receptacle with M2 thread
Titanium

RM2

Receptacle with M3 thread
Titanium

RM3

Receptacle with M3 thread
Peek plastic

RM3(P)

Rectangular Magnetic Receptacle
Titanium

RMR

See receptacle section for details on mass and dimensions.

Optogenetics catalog 7.5 - Fiber-optic Cannula

67

Dual Fiber-optic Cannula
A dual fiber-optic cannula provides two implantable fibers at a precise
distance within a single ferrule. The tolerance on the protrusion for eachfiber is less than 0.1 mm. These cannulas
are perfectly suited for the applicationsGuiding
where two brain centers close to each hole
other
are
simultaneously
optically
stimulated or controlled.
FlangeThe positioning of one mono fiber cannula
FerruleFiberat a time with the stereotaxic equipment
opticshas greater likelihood of 3D positioning
errors (lateral and depth). Additionally, the diameter of the ferrules limits
the minimum distance between the fiber tips. With dual fiber-optic cannula
the insertion of the fiber is faster (single shot), the distance between thefiber tips is predefined and the protrusion depth is assured. The cannula
includes a guiding hole to insure precise alignment when connecting to adual fiber-optic connector (equipped with a guiding pin). The dual fiber
cannula can be made for any distance in 0.7 to 1.7 mm range. If larger
distances between the brain centers need to be covered, please refer to
Two Ferrules Cannulas.
Our dual fiber-optic cannula has a typical transmission higher than 75%
for each fiber.

ORDERING CODE: DFC_□□□/□□□-□□□_□□□_□□□_□□□
Fiber core diameter (µm)
See Table 7 and Table 8
Length “L”(mm)See convention in Table 11
Receptacle code:
See Table 11
Fiber Termination code:
See Table 9 for available codes

Optogenetics catalog 7.5 - Fiber-optic Cannula

68

Table 11: Receptacle codes for dual fiber cannula“Pitch” = Distance between thefibers (mm)0.7

PictureReceptacle
code

L

DF0.7

1.0mm

DF1.0

1.2mm

DF1.2

1.5mm

DF1.5

Select distance (x) in 0.7 mm – 1.7 mm
range

DFx

Optogenetics catalog 7.5 - Fiber-optic Cannula

69

Two Ferrules Cannula
The two ferrules cannula provides two implantable fibers, each within
its own ferrule, at a precise distance exceeding 1.7 mm. The tolerance on
the protrusion for each fiber is less than 0.1 mm. These cannulas are
perfectly suited for the applications where two brain centers at a distancelarger than 1.7 mm from each other are optically stimulated or controlled.
The positioning of one mono fiber cannula at a time with the stereotaxic
equipment has greater likelihood of 3D positioning errors (lateral anddepth). With two ferrules cannula the insertion of the fiber is faster (singleshot), the distance between the fiber tips is predefined and the protrusion
depth is assured.
Two types of receptacles are currently available for the two ferrule
cannula (see pictures on next page). They both consist of precision
machined holders that house zirconia ferrules and determine the spacing
between the ferrules centers. First type of two ferrule cannula connects toa pair of patch cords terminated with ferrules 1.25mm by using two
zirconia sleeves (ID 1.25mm). In the other case, the holder also includes apair of magnets, so that the cannula can connect to a pair of rectangularmagnetic connectors.
The two ferrules cannula can be made for distances larger than 1.7
mm. For shorter distances between the brain centers, please refer to Dualfiber-optic Cannulas.

ORDERING CODE: TFC_□□□/□□□-□□□_□□□_□□□_□□□
Fiber-optic code
See Table 7 and Table 8
Length of fiber “L”(mm)Receptacle code:
See Table 12
Fiber Termination code:
See Table 9 for available codes
NOTE : Unless otherwise specified, an aluminum housing and 1.25mm
zirconia ferrules are being used.

Optogenetics catalog 7.5 - Fiber-optic Cannula

70

Table 12: Termination codes for two ferrules cannulaCenter-to-center
distance between
ferrules (mm)PictureTermination
code

Sleeve connection
2.0 mmTF2

2.5 mmTF2.5

3.0 mmTF3

3.5 mmTF3.5

4.0 mmTF4

Other (x)TFx

Magnetic connection
3mm

TM3

4mm

TM4

Other (x)TMxOptogenetics catalog 7.5 - Fiber-optic Cannula

Fiber-optic Cannula Arrays
Basically this product can be described as a loose fiber optic
bundlewith FC/PC connectors on loose end and V-groove based
fiber-optic cannula array on the other end.

ORDERING CODE:FCA1x8_□□□/□□□-□□□_□□□_□□□_□□□_□□□
Fiber-optic code
See Table 7 and Table 8
Protrusion of fiber “L”(mm)Fiber-optic pitch (um):
See Table 12
Overall fiber length (mm)Termination Code for Multiple connectors side
See Table 2 for available codes71

Optogenetics catalog 7.5 - Fiber-optic Cannula72

Hybrid Cannula
As convergence of different techniques for cell monitoring(optogenetics, electrophysiology) and fluid administration gathers speed,
we are determined to facilitate this trend by providing photonics hardware
products such as new hybrid cannula types.

Optic & Fluid Cannula
The basic idea behind the optogenetics is the introduction of the virus
born proteins like channelrhodopsin-2 to targeted cells or neurons and theillumination of the same through fiber optic tip. So far this has been a two
step process with two different cannulas with inherent imprecision. Led bya request from Brain Science Institute, RIKEN in Japan, we have designed
a hybrid cannula with a metal tube that guides the optical fiber andrestricts liquid delivery around the fiber tip. The design of the hybrid
cannula in one version is based on an M3 receptacle, a metal tube and aside hole to receive liquid injection tube. In other incarnations of hybrid
cannula we use our rectangular magnetic receptacle and similar side
opening for receiving the injection tube. If users want to target the cells
around the fiber tip, the length of the tube and the length of the fiber haveto be the same.
All our mono fiber-optic cannulas have a typical transmission higher than80%.

ORDERING CODE:

OFC_□□□/□□□-□□□_□□□_□□□_□□□
Fiber-optic code
See Table 7 and Table 8
Length “L” (mm)See convention in Table 13
Receptacle code
See Table 13 for available codes
Fiber Termination code
See Table 9 for available codes

Optogenetics catalog 7.5 - Fiber-optic Cannula

73

More information on www.optogenetics-at-doric.com.
Table 13: Receptacle codes for Optic & Fluid Cannula
Description

Termination
code

PictureL
Receptacle with M3 thread

RM3

L
Rectangular Magnetic Receptacle

RMR

Optogenetics catalog 7.5 - Fiber-optic Cannula

74

Table 14: Technical specifications of fiber-optic cannulas
Part

Mass [mg]

Max OD [mm]Length [mm]1.25mm ferrules
Zirconia ferrule 1.25mm

50

1.25

6.5

Zirconia ferrule with MU flange

120

2.5

12

Metal ferrule 1.25mm

50

1.25

6.5

350

2.5

10.5

Not measured

4.5

16

400

2.5

10 or 12.5

Not measured

4.0

10 + 1.5 (pin)2.5mm ferrules
Zirconia ferrule 2.5mm
Zirconia ferrule 2.5mm with FC flange
Metal ferrule 2.5mm
Dual ferrule 2.5mm

Sleeves
Zirconia sleeve ID=1.25mm

20

1.6

6

Zirconia sleeve ID=2.5mm

80

3.2

12

M2 receptacle - titanium

?

2

6

M2 receptacle - plastic

?

2

6

M2 screw - titanium

?

4

3.2

M2 screw - plastic

?

4

3.2

M2 protective cap

?

4

2.5

M3 receptacle - titanium

300

4

7.6

M3 receptacle - plastic

100

4

7.6

M3 screw - titanium

90

4

4.5

M3 screw - plastic

30

4

4.5

M3 protective cap

40

4

4.5

M2

M3

Rectangular magnetic
Rectangular Magnetic receptacle

180

5x2

~8

Rectangular Magnetic connector

150

5x2

5

Cannula assemblies
1.25mm Zirconia ferrules (x2) + sleeve

120

1.6

13

1.25mm metal ferrules (x2) + sleeve 1.25

120

1.6

13

2.5mm Zirconia ferrule (x2) + sleeve 2.5mm

780

3.2

21

2.5mm metal ferrules (x2) + sleeve 2.5mm

880

3.2

21

Optogenetics catalog 7.5 - Fiber-optic Cannula
Dual ferrule (x2) + sleeve 2.5mm

75

Not measured

4.5

20

M3 receptacle titanium + M3 screw titanium+ Zirconia ferrule with flange

500

4

~11

M3 receptacle plastic + M3 screw plastic +Zirconia ferrule with MU flange

250

4

~11

Rectangularmagnetic
receptacle
Rectangular magnetic connector

330

5x2

10

+

Optogenetics catalog 7.5 - Fiber-optic Cannula

76

Receptacles for optogenetics
Connecting a fiber-optic cannula and a fiber-optic patch cord requires
matching the receptacle on the cannula side and the connector on thepatch cord end. The receptacle is the female part of the fiber-optic
connection that contains a centered fiber tip, guides the connecting
ferrule to the optical coupling positions and fastens it in place. In fiber
optics, there are many different types of receptacles. However, here weshow only those specific to our optogenetics products like Zirconia sleeve,
M3 and rectangular magnetic receptacles. Other receptacle types like
SMA, FC/PC are too big to be used for fiber-optic cannulas.

Zirconia sleeve as receptacle
The simplest form of receptacle consists of an implantable fiber glued
in a Zirconia or a metal ferrule inserted in a Zirconia sleeve. The matching
ferrule from the patch cord side is simply inserted in the sleeve.
Connecting 1.25mm ferrules with Zirconia sleeve
Connecting dual ferrules with Zirconia sleeve

Two versions of Zirconia sleeve receptacles areavailable:
Inner diameter Outer diameter
Length1.25 mm

1.6 mm

6.8 mm

2.5 mm

3.2 mm

11.4 mm
Zirconia sleeve

ORDERING CODE : SLEEVE_ZR_□□□
Inner diameter(mm)1.25, 2.50

Optogenetics catalog 7.5 - Fiber-optic Cannula77

M2 receptacle
Ø1.25mm

M2 threads

6mm
2.5 mm

Ø2.6mm

M2 receptacle

M2 connector

M3 receptacle
Ø1.25mm

M3 threads

2.6mm

5mm

4mm

Ø4mm

M3 receptacle

M3 connector

Rectangular Magnetic Receptacle

4mm

5 mm

1 mm

Ø1.25mm
5mm
Rectangular magnetic
connector

5 mm
Rectangular magnetic
receptacle

Optogenetics catalog 7.5 - Fiber-optic Cannula

78

Optic and Fluid Cannula with M3 receptacle
Liquid delivery tube

4.76mmØ4.76mm

9.53mm
Optic connector – M3

Optic & fluid receptacle – M3

Optic and Fluid Cannula with rectangular magnetic receptacle

5 mm

6.5 mmLiquid delivery
tube

Ø1.25mm
5 mm
Optic & fluid connector
Rectangular magnetic

5 mm
Optic & fluid receptacle
Rectangular magnetic

Optogenetics catalog 7.5 - Stereotaxic holders and adapters

79

Stereotaxic holders and adapters
Stereotaxic arm (1) is valued for its positioning precision. However,when it comes to positioning the fiber-optic cannula, some of the precision
is lost when attaching or detaching the cannula to the arm. To simplify the
implantation of the cannula and maintain the precision, we havedeveloped a stereotaxic cannula holder (2) and fiber-optic cannula
adapters(3) for attaching our M3 cannulas to the stereotaxic arm.
1

1

1

2

2

2

3

4

3

3

4

4
Fig a: Cannula implantation

1 Stereotaxic arm
2

Stereotaxic
cannula holder

Fig b: Stereotaxic arm
detachment

Fig c: Adapter and holder
detachment

3 Fiber-optic

cannula adapter

4

CannulaOptogenetics catalog 7.5 - Stereotaxic holders and adapters

Stereotaxic Cannula Holder
8-32 threads

6.35mm80

Ø7.95mm

80mm
Stereotaxic holder 1.25
8-32 threads

6.35mmØ9.55mm

80mm
Stereotaxic holder 2.5

ORDERING CODE : SCH_□□□
Ferrule diameter (mm)2.50, 1.25

M3 or M2 receptacle adapter
ORDERING CODE : FCA_□□□_□□□
Attachment diameter
2.50, 1.25
Receptacle adapter
RM2, RM3

Fiber-optic cannula
adapter – 2.5mm

Fiber-optic cannula
adapter – 1.25mm

Optogenetics catalog 7.5 - Stereotaxic holders and adapters

81

Adapters for Stereotaxic holder
We offer adapters to attach our stereotaxic holders on stereotaxic
frames that use 7.9 mm diameter rods as standard. We can offer otheradapters on request.

In-line adapter
This adapter consists of a 7.9mm diameter rod, with 8-32threads at one
end, and 4-40 threads at the other end.

Stereotaxic In-line Adapter

OD7.9mm8-32 screw
Stereotaxic holder

ORDERING CODE : SIA_7.9
Clamp
This adapter is a double clamp, designed to hold a 7.9mm diameter rod on
one side and a 6.35mm diameter rod on the other side.

ID 7.9mm

ID 6.35mm

Stereotaxic arm

ORDERING CODE : SCL_7.9

Clamp

Stereotaxic
holderOptogenetics catalog 7.5 - Accessories

82

Accessories
Table 15: Mating adapters
Description

PictureCode

Zirconia sleeve ID1.25mm

SLEEVE_ZR_1.25

Zirconia sleeve ID2.5mm

SLEEVE_ZR_2.5

Bronze sleeve ID1.25

SLEEVE_BR_1.25

Bronze sleeve ID2.5

SLEEVE_BR_2.5

FC/FC mating adapter

ADAPTER_FC

SMA/SMA mating adapter

ADAPTER_SMA

M3/M3 mating adapter

ADAPTER_M3

Optogenetics catalog 7.5 - Accessories

83

Table 16: Dust caps
Description

SMA
receptacle cap

FC receptacle
cap

PictureCompatibility
Assemblies that include SMA receptacles:•
Mini cubes•
Fiber-optic rotary joints

CAP_SMA

Assemblies that include FC receptacles:•
Mini cubes•
Fiber-optic rotary joints

CAP_FC•
Ferrule 2.5
cap

•

•
Ferrule 1.25
cap

M3 receptacle
cap

Code

•
•

Cannulas or patch cords with ferrules
OD2.5mm (MF2.5, ZF2.5, DFxx)
FC connectors

Cannulas or patch cords with ferrules
OD1.25mm (MF1.25, ZF1.25)
M3 connectors
Rectangular magnetic connectors

Assemblies that include M3 receptacles:•
Mini cubes•
Fiber-optic rotary joints•
Cannulas with M3 receptacles (RM3)

All products listed in the table are supplied with dust caps.

CAP_Ferrule_2.5

CAP_Ferrule_1.25

CAP_M3

Optogenetics catalog 7.5 - Accessories

84

M3 connectors
M3 connectors offer a secured, light and small connection for
multimode fibers. The standard material of the flange and the screw istitanium. Alternative to titanium is the peek plastic.

M3 connector - parts included: ferrule, screw, strain relief

ORDERING CODE: CM3_□□□
Ferrule inner diameter (µm)
125, 127, 230, 235, 245, 330

ORDERING CODE: CM3P_□□□
Ferrule inner diameter (µm)
125, 127, 230, 235, 245, 330
The letter P indicate that peek plastic is used instead of titanium.

Optogenetics catalog 7.5 - Accessories

85

Table 17: Holders for fiber-optic rotary joints
Description

PictureCode

Holder for•

FRJ_1x1

Holder_FRJ_small•

FRJ_1x2

Holder_FRJ_large•

FRJ_1x4

Holder for

Holder for ERJ, FRJ_1x2,
FRJ_1x4 or combination of ERJ+ FRJ

Holder_ERJ

All 1x2 and 1x4 fiber-optic rotary joints are supplied with their respective
holders.

Optogenetics catalog 7.5 - Accessories
Description

PictureCleaver86
Code

CleaverCablesDescription

M8 male / M8 female,
1.5m long

PictureCode

Cable_M8-M8

Optogenetics catalog 7.5 - Optogenetics kits

87

Optogenetics kits
Until recently, the optogenetics hardware was sold mainly in a modular
fashion or at component level. However, for your convenience, we haveintroduced kits that contain necessary bits and pieces to perform certainclass of experiments. Those kits simplify ordering process and represent a
better deal than buying the same components individually.

Optogenetics beginner’s kit
The beginner’s kit is made up of the single channel LED driver, a single
blue LED diode and rotary joint assembly (LED+FRJ), four types 0.5 m
long mono fiber patch cords and 10 fiber-optic implant cannulas for eachpatch cord type and one dual branching patch cord for bi-lateral activation.
On top of all this, there are zirconia sleeves of 1.25 mm and 2.5 mm innerdiameter and a cleaver. This is what we believe to be the basic photonics
hardware required to perform optogenetics experiments with freely moving
animals.
Optogenetics Beginner’s Kit

Item code Qty
LEDRV_1CH_1000

1

LEDFRJ-B_FC

1

MFP_200/220/900-0.53_0.5_FC-ZF1.25(F)1Single channel LED driverSingle Blue Led + Fiber-optic Rotary Joint
Mono Fiber Patch cord
CannulaMono Fiber Patch cord
CannulaMono Fiber Patch cord
CannulaMono Fiber Patch cord
Cannula

MFC_200/245-0.53_50_ZF1.25_FLT 10
MFP_200/220/900-0.53_0.5_FC-ZF2.5

1

MFC_200/245-0.53_50_ZF2.5_FLT 10
MFP_200/220/900-0.53_0.5_FC-CM31

MFC_200/245-0.53_50_RM3_FLT 10
MFP_200/220/900-0.53_0.5_FC-RMC

1

MFC_200/245-0.53_50_RMR_FLT

10Optogenetics catalog 7.5 - Optogenetics kits

88

Branching Fiber Patch Cord BFP(2)_200/220/900_0.53_100_FC_2xZF1.25(F)2

Zirconia sleeves 1.25 mm

SLEEVE_ZR_1.25 10

Zirconia sleeve 2.5 mm

SLEEVE_ZR_2.5

Cleaver

10
1

ORDERING CODE: OBK
N.B.: The length of fiber-optic implant is 50mm and can be cleaved todesired length by user. The length of fiber protruding from cannula andlength of the patch cord can be changed to whatever length suits yourexperiment.

Optogenetics intermediary level kit
The intermediary kit is made up of a blue and amber dual LED fiberoptic source and a rotary joint, all within a single box that can beconveniently placed on the top of the cage. In addition, four 0.5 m longfiber-optic patch cords are provided to match 4 different types of fiber-optic
implant (cannula) sets with 5 cannulas in each set. This photonics
hardware enables light activation and silencing in optogenetics
experiments with freely moving animals.

ORDERING CODE: OIK
N.B.: The length of fiber-optic implant is 50mm and can be cleaved todesired length by user. Alternatively you may indicate the desired fiber
length.
Optogenetics Intermediary Kit

Item code

Qty

Two channel LED driver

LEDRV_2CH_1000

1

Dual Led + Fiber-optic Rotary Joint

LEDFRJ-B_A_FC

1

MFP_200/220/900-0.53_0.5_FC-ZF1.25(F)1

MFC_200/245-0.53_50_ZF1.25_FLT

10Blue and amber LED light source with fiber-optic rotary joint

Mono Fiber Patch cord
CannulaOptogenetics catalog 7.5 - Optogenetics kits

Mono Fiber Patch cord

MFP_200/220/900-0.53_0.5_FC-ZF2.5

1

MFC_200/245-0.53_50_ZF2.5_FLT

10

MFP_200/220/900-0.53_0.5_FC-CM31

MFC_200/245-0.53_50_RM3_FLT

10

MFP_200/220/900-0.53_0.5_FC-RMC

1

MFC_200/245-0.53_50_RMR_FLT

10CannulaMono Fiber Patch cord
CannulaMono Fiber Patch cord

89

CannulaBranching Fiber Patch Cord

BFP(2)_200/220/900_0.53_100_FC_2xZF1.25(F)2

Zirconia sleeves 1.25 mm

SLEEVE_ZR_1.25

10

Zirconia sleeve 2.5 mm

SLEEVE_ZR_2.5

10

Cleaver1

Optogenetics catalog 7.5 - Optogenetics kits

90

Optogenetics professional kit
The professional kit includes the latest photonics hardware required toperform optogenetics experiments with freely moving animals. Basically,
we have added electrical rotary joint with 12 electrical contacts to ourintermediary kit.

ORDERING CODE: OPK
N.B.: The length of fiber-optic implant is 50mm and can be cleaved todesired length by user. Alternatively you may indicate the desired fiber
length.
Optogenetics Professional Kit
Item code

Qty
LEDRV_2CH_1000

1

Dual Led + Fiber-optic Rotary Joint
LEDFRJ-B/A_FC
Blue and amber LED light source with fiber-optic rotary joint

1

Electrical Rotary Joint

ERJ_12_HRW-HRW

1

Mono Fiber Patch cord

MFP_200/220/900-0.53_0.5_FC-ZF1.25(F)1

MFC_200/245-0.53_50_ZF1.25_FLT

10

MFP_200/220/900-0.53_0.5_FC-ZF2.5

1

MFC_200/245-0.53_50_ZF2.5_FLT

10

MFP_200/220/900-0.53_0.5_FC-CM31

MFC_200/245-0.53_50_RM3_FLT

10

MFP_200/220/900-0.53_0.5_FC-RMC

1

MFC_200/245-0.53_50_RMR_FLT

10

Two channel LED driver

CannulaMono Fiber Patch cord
CannulaMono Fiber Patch cord
CannulaMono Fiber Patch cord
CannulaBranching Fiber Patch Cord

BFP(2)_200/220/900_0.53_100_FC_2xZF1.25(F)2Optogenetics catalog 7.5 - Optogenetics kits

91

Zirconia sleeves 1.25 mm

SLEEVE_ZR_1.25

10

Zirconia sleeve 2.5 mm

SLEEVE_ZR_2.5

10

Cleaver1

Optogenetics catalog 7.5 - Single-cell fiber-optrode cannula kit

92

Single-cell fiber-optrode cannula kit
Doric Lenses presents a first commercially available kit for single-cell
activity recording with fiber-optrodes. These optrodes allow for in-vivo
single-cell combined electrophysiological recordings and optical excitationand monitoring. The opto-electrical interface area is a tip of the fiberoptrode that is made smaller than the cell itself to assure sufficient spatial
resolution.

Source: LeChasseur et al., Nat. Methods. 8, 319–325, 2011

Optogenetics catalog 7.5 - Single-cell fiber-optrode cannula kit

The fiber-optrode probe is madefrom a dual-core optical fibre with
one core filled with electrolyte
serving as an electrode while thehigh-index glass core guides
excitation and fluorescent light. The
initial dual-core optical fiber has 900µm outer diameter, 500 µm opticalguiding core and 100 µm hollow core.
One end of that fiber is pulled tosmaller diameter to create a longtaper that can be cut to diameter sizethat fits the targeted cell size.
Typically this diameter is around 15µm. The custom designed hybrid
connector assures good optical
coupling
andproperelectricalconnection with fiber-optrode probe.
This technique1 has great potential
for single cell identification, local
fluorescencemonitoringandoptogenetics applications.

93

Optogenetics catalog 7.5 - Single-cell fiber-optrode cannula kit

94

ORDERING CODE: SFK
Single-cell Fiber-optrode Cannula Kit
Single LED fiber-optic light-source

Item code Qty
LEDFL_B_FC

1

Low-noise GFP Fluorescence cube

1

Fiber-optic patch cord connection to light source

1

Fiber-optic patch cord connection to reference photodiodeFiber-optic patch cord connection to fluorescence detector

1Fiber-optic patch cord connection to fiber-optrode cannula

1

Fiber-optrode cannula2

Fiber-optrode probe
Piece of dual-core optical fiber with 900 um diameter with one end 100
pulled to 15 um diameterContact us for more information on measurement and assembly
procedure.