HPLC applicationnote & productinformation

HPLC application note &product information
Avantor® offers a full range of materials and consumables for biopharma, healthcare, education,
advanced technology and applied materials industries. We combine a broad portfolio of our own
quality, trusted brands as well as access to millions of other products.
Brands that belong to Avantor (for HPLC market)
J.T.Baker High quality and various reference for HPLC Solvent
Macron Fine Chemicals: Consistent and reliable chemicals
VWR Chemicals BDH: Various products and high quality for LCMS Sovlent (EU Base)
Rankem: Competitive price for HPLC Solvent
VWR Consumables: High quality, various product list for HPLC Consumable
Avantor® ACE® and Avantor® Hichrom: High quality range of U/HPLC chromatography products

Avantor’s portfolio of manufactured
U/HPLC columns offers:‒‒ High quality products‒‒ Competitive prices‒‒ Fast delivery‒‒ Expert technical support‒‒ Excellent after-sales care

2

HPLC application note & product information | vwr.com

HPLC analysis method
development service
Avantor provides the well-organized method development andvalidation platform in accordance with ICH and state-of-the-art
technique to be able to help establishment of your quality attribute.
Core Capability‒‒ HPLC analytical method development‒‒ Biochemical analysis method development‒‒ Product stability test by ICH‒‒ Optimization‒‒ Method transfer

Method development Procedure

Non
Disclosure
Agreement

Consulting

Document
Support

Methoddevelopment

Report(optional)

Service Industry
Bio/Pharmaceuticals

Amino acids, Peptide, Proteins

ClinicalHormones, Drug metabolites, Poisons

Industrial Chemicals

Petrochemicals, Electronic materials, Synthesized chemicals

Food

Preservatives, Health supplement, Hair dye, Pigments

Environment

Contaminants, Pesticide, Herbicide

Cosmetics

Cosmeceuticals, Preservatives, Hair dye

Materials in R&D Step

vwr.com | HPLC application note & product information

3

FOOD

WATER SOLUBLE VITAMINS (1)Pyridoxamine
Thiamine (Vit B1)
3. L-Ascorbic acid (Vit C)
4. Niacinamide (Vit B3)
5. Nicotinic acid
6. Pyridoxal
7. Pyridoxine

P-Aminobenzoic acid
Pantothenic acid (Vit B5)
10. Folic acid (Vit B9)
11. Cyanocobalamin (Vit B12)
12. Rivoflavin (Vit B2)
13. d-Biotin(Vit B7)
14. Thioctic acid

1.

8.

2.

9.

Column : ACE 5 C8 (4.6*250mm)
Mobile phase : Gradient analysis
A : 50mM KH2PO4 pH 2.5
B : MeOH
Flow rate: 1 mL/min
Temperature: Ambient
Detector: UV, 205 nm

Time (mins)

%B

0

0

3

0

16.5

45

19.5

80

WATER SOLUBLE VITAMINS (2)Pyridoxine (Vit B6)
p-Aminobenzoic acid
3. Pantothenic acid
4. Folic acid
5. d-Biotin
6. Cyanocobalamin
7. Riboflavin
1.

2.

Column: ACE Ultracore 2.5
SuperPhenylHexyl (2.1*50 mm)
Mobile phase : Gradient analysis
A : 20mM KH2PO4, pH 2.7
B : 20mM KH2PO4, pH 2.7 in MeOH/
Water(50:50 v/v)
Flow rate: 0.4 mL/min

4

HPLC application note & product information | vwr.com

Injection vol.: 1 μL

Time (mins)
0

%B
20

Temperature: 40 °C

1.5

60

Detector: UV, 205
and 254nm

3

70

3.75

70

4.5

20

FOOD

SUGARS-MONOSACCHARIDES AND DISACCHARIDES
Fructose
Glucose3. Sucrose
1.

2.

Column : ACE Excel 3 NH2 (4.6*150mm)
Mobile phase : ACN : Water (75:25 v/v)
Flow rate: 1.0 mL/min
Injection vol.: 10μL
Temperature: 35 °C ℃
Detector: RID, 35

MONOSACCHARIDES ANALYSISDISACCHARIDES ANALYSIS

Column : ACE Excel 5 NH2 (4.6*150mm)

Column : ACE Excel 3 NH2 (4.6*150mm)

Mobile phase : ACN : Water (75:25 v/v)

Mobile phase : ACN : Water (80:20 v/v)

Flow rate: 1.0 mL/min

Flow rate: 1.0 mL/min

Injection vol.: 10μL

Injection vol.: 20μL

Temperature: 35 °C

Temperature: 35 °C ℃

Detector: RID, 35

Detector: RID, 35℃

vwr.com | HPLC application note & product information

5

FOOD

SWEETENERS AND PRESERVATIVES IN ENERGY DRINK
Pyridoxine (Vit B6)
Citric acid
3. Pantothenic acid (Vit B5)
4. Acesulfame K
5. Caffeine
6. Aspartame
7. Sorbate
8. Benzoate
1.

2.

Column : ACE Excel 3 C18-mide (4.6*150mm)
Mobile phase : Gradient Analysis
A: 0.1% Phosphoric Acid in Water
B: 0.1% Phosphoric Acid in ACN
Flow rate: 1.0 mL/min

HPLC application note & product information | vwr.com%B

Injection vol.: 5μL

0

0

12

65

Temperature: 30 °C

15

95

16

95

16.5

5

30

5

Detector: UV, 214nm

6Time (mins)

FOOD

FAST SEPARATION OF ORGANIC ACIDS
Sinapic acid
Ferulic acid
3. 3,4-Dimethoxycinnamic acid
4. Cinnamic acid
5. 4-Methoxycinnamic acid1.

2.

Column : ACE Excel 3 C18-mide (2.1*250mm)
Mobile phase : 20mM H3PO4 n MeOH/
Water (40:60 v/v)
Flow rate: 0.21 mL/min
Injection vol.: 5μL
Temperature: 20 °C
Detector: UV, 210nm

Column : ACE Excel 1.7 C18Amide (3.0*50mm)
Mobile phase : 20mM H3PO4 in MeOH/
Water (40:60 v/v)
Flow rate: 0.8 mL/min
Injection vol.: 2μL
Temperature: 20 °C ℃
Detector: UV, 210nm

vwr.com | HPLC application note & product information

7

FOOD

FATTY ACID ANALYSIS
Linolenic acid
Myristic acid
3. Linoleic acid
4. Palmitic acid
5. Oleic acid
6. Stearic acid1.

Column : ACE 3 C8 (4.6*150mm)

2.

Mobile phase : ACN/Water (90:10 v/v)
Flow rate: 1.0 mL/min
Injection vol.: 5μL
Temperature: 15 °C
Detector: ELSD, 22

PRODUCT LIST
Description

Product Name

Product Number

Brand

ColumnACE 5 C8

ACE-122-2546

ACE

ColumnACE Ultracore 2.5 SuperPhenylHexyl

CORE-25B-0502U

ACE

ColumnACE Excel 3 NH2 (4.6*150mm)

EXL-1114-1546U

ACE

ColumnACE Excel 5 NH2 (4.6*150mm)

EXL-1214-1546U

ACE

ColumnACE Excel 3 C18-Amide (4.6*150mm)

EXL-1112-1546U

ACE

ColumnACE Excel 3 C18-Amide (2.1*250mm)

EXL-1112-2502U

ACE

ColumnACE Excel 1.7 C18-Amide (3.0*50mm)

EXL-1712-0503U

ACE

ColumnACE 3 C8

ACE-112-1546

ACE

Guard Holder

1 cm integral holder for 2,1 mm ID columns

H0004

ACE

Guard cartridge

For 2,1 mm ID columns

ACE-213-0102GD

ACE

Pre-Column Filter

UltraShield Pre-Column Filter

ACE-US1505

ACE

HPLC Grade Solvent

Water

4218-88

J.T.Baker

HPLC Grade Solvent

Acetonitrile

9012-88

J.T.Baker

HPLC Grade Solvent

Methanol9093-88

J.T.Baker

Mobile Phase Additives

Phosphoric Acid0260-01

J.T.Baker

Mobile Phase Additives

Potassium Phosphate Monobasic (KH2PO4)

3246-01

J.T.Baker

8

HPLC application note & product information | vwr.com

COSMETICSFAST SEPARATION OF ORGANIC ACIDS
p-Phenylenediamine
p-Aminophenol
3. Toluene-2,5-diamine
4. m-Aminophenol
5. o-Phenylenediamine
6. 2-Chloro-pphenylenediamine
7. o-Aminophenol
8. Resorcinol
1.

2.

2-Nitro-pphenylenediamine
10. Toluene-3,4-diamine
11. 4-Amino-2-hydroxytoluene
12. 2-Methylresorcinol
13. 6-Amino-m-cresol
14. N,N-Diethyltoluene-2,5diamine
15. 4-Amino-3-nitrophenol
9.

Column: ACE Excel 5 C18-Amide (4.6*250mm)
Mobile phase: A:B=60:40 (v/v) A: 1.8
g disodium phosphate
dodecahydrate+ 2.8 g
potassium dihydrogen
phosphate + 1.0 g sodium
1-heptanesulfonate in
Water(1L)B: ACN
Flow rate: 1.0 mL/min
Temperature: 25 °C
Detector: UV, 280nm

vwr.com | HPLC application note & product information

9

COSMETICSPRESERVATIVES
Phthalic acid
p-Hydroxybenzoic acid
3. Benzoic acid
4. Sorbic acid
5. Methyl paraben
6. Ethyl paraben
7. Propyl paraben
1.

2.

Column: ACE 5 C18, (4.6*250mm)
Mobile phase: ACN/50mM KH2PO4 pH 4.4 in
Water (40:60 v/v)
Flow rate: 1.0 mL/min
Temperature: Ambient
Detector: UV, 230nm

WATER SOLUBLE VITAMINS (2)Phthalic acid
p-Hydroxybenzoic acid
3. Sorbic acid
4. Methyl paraben
5. Ethyl paraben
6. Propyl paraben
1.

2.

Column: ACE Excel 1.7 C18 (3.0*50mm)
Mobile phase: ACN/20mM KH2PO4 pH 2.5 in
Water (30:70 v/v)
Flow rate: 0.43 mL/min
Temperature: 20 °C
Detector: UV, 230nm

10 HPLC application note & product information | vwr.com

COSMETICSSUN SCREEN AGENTS
Benzophenone-3
Octocrylene
3. Octyl dimethyl PABA
4. Octyl methoxycinnamate
5. Avobenzone
6. Ethylhexyl salicylate
7. Homosalate
8. Ethylhexyl triazone
9. Tinosorb® M
10. Tinosorb® S
1.

Column: ACE 3 C18, 4.0*150mm, 3 μm

2.

Mobile phase: Gradient analysis
A: MeOH/Water (85:15 v/v)
B: THF
Flow rate: 0.85 mL/min

Time (mins)

%B

0

0

Temperature: : 30 °C

7

0

10

50

Detector: UV, 310nm

14

50

16

0

PRODUCT LIST
Description

Product Name

Product Number

Brand

ColumnACE Excel 5 C18-Amide

EXL-1212-2546U

ACE

ColumnACE 5 C18

ACE-121-2546

ACE

ColumnACE Excel 1.7 C18

EXL-171-0503U

ACE

ColumnACE 3 C18

ACE-111-1504

ACE

Guard Holder

1 cm integral holder for 2,1 mm ID columns

H0004

ACE

Guard cartridge

For 2,1 mm ID columns

ACE-213-0102GD

ACE

HPLC Grade Solvent

Water

4218-88

J.T.Baker

HPLC Grade Solvent

Acetonitrile

9012-88

J.T.Baker

HPLC Grade Solvent

Methanol9093-88

J.T.Baker

HPLC Grade Solvent

THF

9440-88

J.T.Baker

Mobile Phase Additives

Sodium Phosphate Dibasic, 12-Hydrate3822-01

J.T.Baker

Mobile Phase Additives

Potassium Phosphate Monobasic (KH2PO4)

3246-01

J.T.Baker

Mobile Phase Additives

1-Heptanesulfonic Acid, Sodium Salt

2173-01

J.T.Baker

vwr.com | HPLC application note & product information 11

ENVIRONMENTPESTICIDE & HERBICIDE ANALYSIS
Deisopropylatrazine
Desethylatrazine
3. Simazine
4. Cyanazine
5. Atrazine
6. Internal Standard
7. Sebuthylazine

Propazine
Terbuthylazine
10. Prometryn
11. Terbutryn
12. Alachlor
13. Pendimethalin

1.

8.

2.

9.

Column : ACE 3 C18 (2.1*150mm)
Mobile phase : Gradient analysis
A: 0.1M Ammonium Acetate in Water
B: ACN
Flow rate: 0.3 mL/min
Injection vol.: 25μL
Temperature: 40 °C
Detector: UV, 220 nm(Pendimethalin at 245nm)

Phenolo-Cresol
3. 2-Chlorophenol
4. 4-Chlorophenol
5. 2,6-Dichlorophenol
6. 6-CP
7. 2,4-D

MCPA
PCOC
10. 2,4-Dichlorophenol
11. 2,4-DP
12. CMPP
13. 2,4-DB
14. MCPB

1.

8.

2.

9.

Time (mins)

%B

0

10

40

80

47

90

49

10

Column : ACE 3 C18-PFP (4.6*150mm)
Mobile phase : Gradient analysis
A: 0.1% Formic Acid in Water
B: MeOH
Flow rate: 1.0 mL/min
Injection vol.: 10μL

12 HPLC application note & product information | vwr.com

Temperature: 35 °C

Time (mins)

Detector: UV, 280 nm

0

10

20

100

%B

ENVIRONMENTPHENOLIC COMPOUNDS IN GROUND WATER AND SOIL
Pyrocatechol
2.Resorcinol
3. 3.Phenol
4. 4.m-Cresol
5. 5.o-Cresol
6. 6.2,4-Dimethylphenol
7. 7.3,4-Dimethylphenol

8.3,5-Dimethylphenol
9.1-Naphthol
10. 10.3,4,5-Trimethylphenol
11. 11.2,3,6-Trimethylphenol
12. 12.2,4,6-Trimethylphenol
13. 13.2-Naphthol

1.

8.

2.

9.

Column : ACE Excel 3 C18-Amide (4.6*150mm)
Mobile phase : 0.1% Formic acid in
ACN/Water (35:65 v/v)
Flow rate: 1.0 mL/min
Injection vol.: 10μL
Temperature: 30 °C
Detector: UV, 274 nm

EXPLOSIVE ANALYTES
1,3,5-Trinitrobenzene
Nitrobenzene
3. 1,3-Dinitrobenzene
4. 2,4-Dinitrotoluene
1.

2.

Column : ACE Excel 5 CN-ES (4.6*150mm)
Mobile phase : MeOH/Water (50:50 v/v)
Flow rate: 1.0 mL/min
Injection vol.: 5μL
Temperature: 20°C
Detector: UV, 254 nm

vwr.com | HPLC application note & product information 13

ENVIRONMENTPAHS BEYOND THE EPA PRIORITY POLLUTANTS
Naphthalene
Acenaphthylene
3. 1-Methylnaphthalene
4. 2-Methylnaphthalene
5. Acenaphthene
6. Fluorene
7. Phenanthrene
8. Anthracene
9. Fluoranthene
10. Pyrene
11. Benzo[c]phenanthrene
12. Cyclopenta[cd]pyrene
13. Benz[a]anthracene

14. Chrysene

1.

15. Benzo[b]naptho[2,1-d]

2.

Column : Vydac 218TP54 (4.6*150mm)

thiopen
16. 7,12-Dimethylbenz[a]anthracene
17. Benzo[e]pyrene
18. Benzo[b]fluoranthene
19. Benzo[k]fluoranthene
20. Benzo[a]pyrene
21. Dibenz[ah]anthracene
22. Benzo[ghi]perylene
23. Indeno[1,2,3-cd]pyrene

Mobile phase : A: Water, B: ACN
Gradient: 50 to 100 %B over 30mins
Flow rate: 1.0 mL/min
Temperature: 30 °C
Detector: UV/Vis, 254 nm

PRODUCT LIST
Description

Product Name

Product Number

Brand

ColumnACE 3 C18

ACE-111-1502

ACE

ColumnACE 3 C18-PFP

ACE-1110-1546

ACE

ColumnACE Excel 3 C18-Amide

EXL-1112-1546U

ACE

ColumnACE Excel 5 CN-ES

EXL-1213-1546U

ACE

ColumnVydac 201TP

201TP5415

Vydac

Guard Holder

1 cm integral holder for 2,1 mm ID columns

H0004

ACE

Guard cartridge

For 2,1 mm ID columns

ACE-213-0102GD

ACE

HPLC Grade Solvent

Water

4218-88

J.T.Baker

HPLC Grade Solvent

Acetonitrile

9012-88

J.T.Baker

HPLC Grade Solvent

Methanol9093-88

J.T.Baker

Mobile Phase Additives

Formic acid0128-01

J.T.Baker

Mobile Phase Additives

Ammonium Acetate

3272-04

J.T.Baker

14 HPLC application note & product information | vwr.com

PROTEIN/PEPTIDEGRADIENT SEPARATION OF PEPTIDE AND PROTEINS
Compound information
Oxytocin
Bradykinin
3. Angiotensin ll
4. Angiotensin l
5. Ribonuclease A
6. Cytochrome C
7. Bovine Insulin
8. Human Insulin
9. Porcine Insulin
10. Lysozyme
1.

2.

1007 Da
1060 Da
1046 Da
1296 Da
13.7 kDa
12.3 kDa
5733 Da
5808 Da
5777 Da
14.3 kDa

Analysis Condition
Column : ACE 3 C4-300 (2.1*150mm)
Mobile phase : Gradient analysis
A : 0.1 % TFA in Water
B : 0.1 % TFA in ACN : Water (80 : 20 v/v)
Flow rate: 0.5mL/min
Injection vol.: 3μL
Temperature: 60 °C
Detector: UV, 220nm

Time (mins)

%B

0

10

15

50

17.5

50

18

10

vwr.com | HPLC application note & product information 15

ENVIRONMENTRAPID SEPARATION OF PEPTIDES
Compound information
Gly-Tyr (Mw 238.2)
Val-Tyr-Val (Mw 379.5)
3. Tyr-Gly-Gly-Phe-Met(Met‑Enkephalin, Mw 573.7)
4. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe(Angiotensin ll, Mw 1046.2)
5. Tyr-Gly-Gly-Phe-Leu(Leu Enkephalin, Mw 555.5)

Analysis Condition

1.

2.

Column: ACE Excel 1.7 C18, 3.0*50mm, 1.7 μm
Mobile phse: Gradient analysis
A: 0.05% TFA in Water
B: 0.05% TFA in ACN
Flow rate: 0.8mL/min
Injection vol.: 1μL
Temperature: 60°C
Detector: UV, 220nm

16 HPLC application note & product information | vwr.com

Time (mins)

%B

0

5

3.0

40

4.0

90

5.5

90

10.5

5

PROTEIN/PEPTIDEANALYSIS OF BOVINE, HUMAN, PORCINE INSULIN
Bovine Insulin
Human Insulin
3. Porcine Insulin
1.

Column: ACE 5 C18, (4.6*250mm)

2.

Mobile phase: Gradient analysis
A: 0.1% TFA in Water/ACN (71:29 v/v)
B: 0.1% TFA in Water/ACN (68:32 v/v)
Flow rate: 1 mL/min
Injection vol.: 10μL
Temperature: Ambient

Time (mins)

Detector: UV, 215nm

%B

0

10

10

90

CATECHOLAMINES AND METANEPHRINES SEPERATION
Norepinephrine
Epinephrine
3. Normetanephrine
4. Dopamine
5. Metanephrine
1.

2.

Column : ACE Excel 5 CN-ES(4.6*150mm) 5μm
Mobile phase : MeOH/Water (50:50 v/v)
Flow rate: 1.0 mL/min
Injection vol.: 5μL
Temperature: 25 °C
Detector: UV, 260 nm

vwr.com | HPLC application note & product information 17

PROTEIN/PEPTIDEMILK PROTEINSColumn: ACE 5 C18-300, (2.1*150mm)
Mobile phase: Gradient analysis
A: 0.1% TFA in Water
B: 0.1% TFA in ACN
Flow rate: 0.2 mL/min
Temperature: 45
Detector: UV, 214nm

Time (mins)

%B

0

33

5

33

9

35

18

37

22

40

27.5

41

28

41

43

43

PRODUCT LIST
Description

Product Name

Product Number

Brand

ColumnACE 3 C4-300 (2.1*150mm)

ACE-213-1502

ACE

ColumnACE Excel 1.7 C18 (3.0*50mm)

EXL-171-0503U

ACE

ColumnACE 5 C18 (4.6*250mm)

ACE-121-2546

ACE

ColumnACE 5 C18-PFP (4.6*150mm)

ACE-1210-1546

ACE

ColumnACE 5 C18-300 (2.0*150mm)

ACE-221-1502

ACE

Pre-Column Filter

UltraShield Pre-Column Filter

ACE-US1505

ACE

HPLC Grade Solvent

Water

4218-88

J.T.Baker

HPLC Grade Solvent

Acetonitrile

9012-88

J.T.Baker

Mobile Phase Additives

Trifluoroacetic acid ≥99.5% for HPLC

9470-00

J.T.Baker

18 HPLC application note & product information | vwr.com

CLINICAL/DRUGSANALGESIC SEPARATION

Column: ACE 3 C18-AR (4.6*50mm)
Mobile phase: Gradient analysis
A: 0.1% Formic acid in Water
B: 0.1% Formic acid in ACN
Flow rate: 1.0 mL/min

Time (mins)%B

Temperature: 40°C

0

5

9

35

Detector: UV, 240nm

1435

4-Acetamidophenol
4-Aminobenzoic acid
3. 4-Hydroxybenzoic acid
4. Caffeine
5. 2-Acetamidophenol
6. 3-Hydroxybenzoic acid
7. Salicylamide
8. Acetanilide
1.

2.

9.

Phenol

10. Acetylsalicylic acid
11. Benzoic acid
12. Sorbic acid

13. Salicylic acid14. Phenacetin

15. Salicylaldehyde

Column: ACE Excel 2 SuperC18, (2.1*50mm)
Mobile phase: Gradient analysis
A: 0.1% Formic acid in Water
B: 0.1% Formic acid in ACN
Flow rate: 1.2 mL/min
Injection vol.: 0.5μL
Temperature: 50 °C
Detector: UV, 214nm

Time (mins)

%B

0

5

1

100

1.5

100

1.6

5

3.1

5

Paracetamol
Hydrochlorothiazide
3. Aspirin
4. Bendroflumethiazide
5. Ketoprofen
6. Flurbiprofen
7. Ibuprofen
1.

2.

vwr.com | HPLC application note & product information 19

CLINICAL/DRUGSANTIHISTAMINES AND EXPECTORANTS
Pseudoephedrine
2. Scopolamine
3. Doxylamine
4. Chlorpheniramine
5. Triprolidine
6. Diphenhydramine
7. Acrivastine
8. Promethazine
9. Fexofenadine
10. Cetirizine
11. Loratadine
1.

Maleic acid
Norephedrine
3. Salicylamide
4. Guaifenesin
5. Guaiacol
6. Chlorpheniramine maleate
7. Dextromethorphan
1.

2.

Column: ACE UltraCore 2.5 SuperC18 (3.0*100mm)
Mobile phase: Gradient analysis
A: 20mM ammonium formate pH 3.0 in Water
B: 20mM ammonium formate pH 3.0 in
MeOH/Water (9:1 v/v)
Flow rate: 1.2 mL/min

Time (mins)
00

Temperature: 25 °C

7.5

100

8.5

100

9.0

0

Detector: UV, 254nm

Column: ACE 5 C18 4.6*250mm, 5 μm
Mobile phase: MeOH/50mM KH2PO4 pH 3.0 in
Water (50:50 v/v)
Flow rate: 1.0 mL/min
Temperature: 22 °C
Detector: UV, 220nm

20 HPLC application note & product information | vwr.com%BCLINICAL/DRUGSSEPARATION OF PENICILLINSβ-BLOCKERS ANALYSIS

1.

Penicillin V
Piperacillin
3. Ampicillin
4. Amoxicillin

1.

2.

2.

Penicillin V
Piperacillin
3. Ampicillin
4. Amoxicillin

Column: ACE 5 HILIC-B (4.6*150mm)

Column: ACE 5 HILIC-A (4.6*150mm)

Mobile phase: 10 mM Ammonium Formate
pH 4.7 in ACN:Water ( 80:20 v/v )Mobile phase: 12 mM Ammonium Formate		Flow rate: 1.5 mL/min

pH 4.7 in ACN:Water ( 88:12 v/v )Flow rate: 1.5 mL/min

Temperature: 30 °C

Temperature: 25 °C

Detector: UV, 214nm

Detector: UV, 230nm

NON-STEROIDAL ANTI-INFLAMMATORY DRUGSColumn: ACE Excel 2 SuperC18 (3.0*50mm)
Mobile phase: Gradient analysis
A: 0.1% Formic acid in Water
B: 0.1% Formic acid in ACNTime (mins)

%B

0

20

Injection vol.: 1.4μL

0.25

20

Temperature: 40 °C

3.5

70

4.0

20

4.25

20

Flow rate: 0.86 mL/min

Detector: UV, 254nm
AspirinPhenacetin
3. Sulindac
4. Tolmetin
5. Naproxen
6. Nimesulide
7. Flurbiprofen
8. Diclofenac
9. Phenylbutazone
10. Meclofenamic acid1.

2.

vwr.com | HPLC application note & product information 21

CLINICAL/DRUGSANALYSIS OF NICOTINE AND RELATED SUBSTANCES

2.

Mobile phase: Gradient analysis
A: 10mM Ammonium Acetate, pH 10.0
B: ACN
Flow rate: 0.6 mL/min
Injection vol.: 5μL
Temperature: 30 °C
Detector: UV, 260nm

Nicotine-cis-N-oxide
Nicotine-trans-N-oxide
3. Norcotinine
4. Cotinine
5. Nornicotine
6. Anatabine
7. Myosmine
8. MNP
9. Anabasine
10. Nicotine
11. β-nornicotyrine
12. β-nicotyrine
1.

Column: ACE Excel 2 SuperC18 (2.1*100mm)

Time (mins)

%B

0

0

8.5

34

10.0

0

11.5

0

22 HPLC application note & product information | vwr.com

CLINICAL/DRUGSBIOMARKER PROFILING

Column: ACE UltraCore 2.5 SuperC18 (2.1*50mm)
Mobile phase: A:B=90:10
A: 7 % Methanol, 2.1 mM Tetrabutylammonium
bisulfate + 84mM KH2PO4, pH 6 with KOH
B: 7% Methanol, 2.1 mM Tetrabutylammonium
bisulfate + 8.4mM KH2PO4, pH 6 with KOH
Flow rate: 0.4 mL/min
Injection vol.: 2μL
Temperature: Ambient

Deoxyuridine
Deoxyguanosine
3. Deoxythymidine
4. Adenosine
5. Deoxyadenosine
6. 2-Fluoro-2’-fluoroadenine
arabinoside
7. 2-Fluorodeoxyadenosine
8. 2-Fluoroadenosine
9. 2-Chlorodeoxyadenosine
10. Clofarabine
11. Methylthioadenosine
1.

2.

Detector: UV, 260nm

vwr.com | HPLC application note & product information 23

CLINICAL/DRUGSSTEROIDS ANALYSIS BY UHPLC

2.

Mobile phase: Gradient analysis
A: 0.1% Formic acid in Water
B: 0.1% Formic acid in ACNTime (mins)

Flow rate: 0.4 mL/min

Cortisone
Corticosterone
3. 11α-hydroxyprogesterone
4. 11-Ketoprogesterone
5. β-Estradiol
6. 17α-Estradiol
7. 17α-Ethinylestradiol
8. Estrone
1.

Column: ACE Excel 2 CN-ES (2.1*50mm)

Temperature: 40 °C
Detector: UV, 260nm

%B

0

25

10

80

10.5

80

11.0

25

Description

Product Name

Product Number

Brand

ColumnACE 3 C18-AR

ACE-119-1546

ACE

ColumnACE Excel 2 SuperC18

EXL-1011-0502U

ACE

ColumnACE UltraCore 2.5 SuperC18

CORE-25A1003U

ACE

ColumnACE 5 C18

ACE-121-2546

ACE

ColumnACE 5 HILIC-B

HILB-5-1546U

ACE

ColumnACE 5 HILIC-A

HILA-5-1546U

ACE

ColumnACE Excel 2 SuperC18

EXL-1011-0503U

ACE

ColumnACE Excel 2 SuperC18

EXL-1011-1002U

ACE

ColumnACE UltraCore 2.5 SuperC18

CORE-25A-0502U

ACE

ColumnACE Excel 2 CN-ES

EXL-1013-0502U

ACE

Pre-Column Filter

UltraShield Pre-Column Filter

ACE-US1505

ACE

HPLC Grade Solvent

Water

4218-03

J.T.Baker

HPLC Grade Solvent

Acetonitrile

9012-03

J.T.Baker

HPLC Grade Solvent

Methanol9093-88

J.T.Baker

Mobile Phase Additives

Trifluoroacetic acid ≥99.5% for HPLC

9470-00

J.T.Baker

Mobile Phase Additives

Formic acid0129-01

J.T.Baker

Mobile Phase Additives

Potassium Phosphate Monobasic (KH2PO4)

3246-01

J.T.Baker

Mobile Phase Additives

Ammonium Acetate

3272-04

J.T.Baker

24 HPLC application note & product information | vwr.com

LC-MS

MYCOTOXINS BY LC-MS/MS

Aflatoxin G2 (m/z 331.1 → 313.1)
Aflatoxin G1 (m/z 329.0 → 243.1)
3. Aflatoxin B2 (m/z 315.1 → 287.0)
4. Aflatoxin B1 (m/z 313.1 → 285.0)
5. HT-2-toxin (m/z 442.2 → 263.1)
6. T-2-toxin (m/z 484.2 → 305.1)
7. Ochratoxin A (m/z 404.1 → 239.0)
1.

Column: ACE Excel 2 C18-AR, 50 x 2.1 mm

2.

Mobile phase: Gradient analysis
A: 1 mM ammonium acetate + 0.5%
Acetic acid acid in Water
B: 1 mM ammonium acetate + 0.5%
Acetic acid in 95% MeOH
Flow rate: 0.6 mL/min
Temperature: 40 °C
Detector: LC-MS/MS

Time (mins)

%B

0

40

1.0

40

2.4

60

6.8

87

vwr.com | HPLC application note & product information 25

LC-MSβ-BLOCKERS BY LC-MS/MS

Column: ACE Excel 2 C18 50 x 2.1 mm
Mobile phase: Gradient analysis
A: 2mM Ammonium acetate + 0.1%
Formic acid in Water
B: 2
 mM Ammonium acetate + 0.1%
Formic acid in MeOH
Flow rate: 0.4 mL/min
Temperature: 40 °C
Injection volume: 10 μL
Detector: LC-MS/MS

Time (mins)

%B

0

10

3.0

50

3.1

10

Sotalol (m/z 272.9 → 212.8)
Atenolol (m/z 267.0 → 89.8)
3. Pindolol (m/z 248.9 → 115.8)
4. Nadolol diastereomers (m/z 310.0 → 253.9)
5. Metoprolol (m/z 268.0 → 115.8)
6. Labetalol (m/z 329.1 → 161.8)
7. Propranolol (m/z 260.0 → 115.7)
8. Alprenolol (m/z 250.0 → 115.8)
1.

2.

26 HPLC application note & product information | vwr.com

LC-MSPERFLUORINATED COMPOUNDS IN WATER BY LC-MS/MS

Column: ACE Excel 1.7 C18 2.1*100mm, 1.7μm EXL-171-1002U
Mobile phase: Gradient analysis
A: 2mM Ammonium acetate, 0.1% Formic acid in Water/MeCN (90:10 v/v)
B: 2mM Ammonium acetate, 0.1% Formic acid in Water/MeCN (10:90 v/v)
Flow rate: 0.5 mL/min

Time (mins)

%B

0

25

Injection vol.: 10μL
Temperature: 40 °C
Detector: LC-MS/MS

0.5

25

3.5

70

4.0

100

5.5

100

6.0

25

9.0

25

Perfluorohexanoic acid (m/z 313.0 → 268.9)
Perfluorobutanesulfonic acid (m/z 299.0 → 79.9)
3. Perfluorooctanoic acid (m/z 413.0 → 368.9)
4. Perfluorohexanesulfonic acid (m/z 399.0 → 80.0)
5. Perfluorononanoic acid (m/z 463.0 → 419.0)
6. Perfluorodecanoic acid (m/z 513.0 → 469.0)
7. Perfluorooctanesulfonic acid (m/z 499.0 → 80.0)
1.

2.

vwr.com | HPLC application note & product information 27

LC-MS

Organophosphorus flame retardants inwater by LC-MS/MS

Column: ACE3 C18 2.1*100mm, ACE-111-1002
Mobile phase: Gradient analysis
A: 0.05mM Ammonium formate, 0.005%
Formic acid in Water
B: Water:MeCN = 95:5 (v/v)
Flow rate: 0.25 mL/min
Injection vol.: 80μL
Temperature: 25 °C
Detector: LC-MS/MS

Time (mins)

%B

0.1

50

12

90

13

100

15

100

15.1

50

20

50

Trimethyl phosphate (TMP) (m/z 141 → 109)
Triethyl phosphate (TEP) (m/z 183 → 127)
3. Tris(2-chloroethyl) phosphate (TCEP) (m/z 285 → 223)
4. Bis(1,3-dichloro-2-propyl) phosphate (BDCP) (m/z 321 → 99)
5. Triiso-propyl phosphate (TiPP) (m/z 225 → 99)
6. Tri-n-propyl phosphate (TPrP) (m/z 225 → 99)
7. Tris((2R)-1-chloro-2-propyl) phosphate (TCPP) (m/z 327 → 99)

Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) (m/z 431 → 99)
Triphenyl phosphate (TPP) (m/z 327 → 215)
10. Tri-n-butyl phosphate (TBP) (m/z 267 → 211)
11. Tris(2-butoxyethyl) phosphate (TBEP) (m/z 399 → 299)
12. Bis(2-ethylhexyl) phosphate (BEHP) (m/z 323 → 99)

1.

8.

2.

9.

28 HPLC application note & product information | vwr.com

LC-MSAMPHETAMINES IN URINE BY LC-MS/MS

Column: ACE Excel 3 SuperC18 75 x 2.1 mm
Mobile phase: Gradient analysis
A: 5mM Ammonium hydroxide (pH 10.8) in Water
B: 5mM Ammonium hydroxide (pH 10.8) in MeOH/
Water (90/10 v/v)
Flow rate: 0.4 mL/min
Temperature: 40 °C

Time (mins)

%B

0

30

8

95

Detector: LC-MS/MS

Phenylpropanolamine (m/z 151.6 → 134.0)(l)-Ephedrine (m/z 166.2 → 148.0)
3. (dl)-3,4-MDA (m/z 179.7 → 163.0)
4. (d)-Amphetamine (m/z 135.8 → 90.9)
5. (dl)-3,4-MDMA (m/z 193.7 → 163.0)
6. 4-Methylthioamphetamine (m/z 182.2 → 165.0)
7. (±)-MDEA (m/z 207.7 → 165.0)
1.

2.

PRODUCT LIST
Description

Product Name

Product Number

Brand

ColumnACE Excel 2 C18-AR (50*2.1mm)

EXL-109-0502U

ACE

ColumnACE Excel 2 C18 (50*2.1mm)

EXL-101-0502U

ACE

ColumnACE Excel 1.7 C18 (100*2.1mm)

EXL-171-1002U

ACE

ColumnACE 3 C18 (100*2.1mm)

ACE-111-1002

ACE

ColumnACE Excel 3 SuperC18 (75*2.1mm)

EXL-1111-7502

ACE

Pre-Column Filter

UltraShield Pre-Column Filter

ACE-US1505

ACE

HPLC Grade Solvent

Water

4218-88

J.T.Baker

HPLC Grade Solvent

Methanol9012-88

J.T.Baker

HPLC Grade Solvent

Acetonitrile

9093-88

J.T.Baker

Mobile Phase Additives

Ammonium Acetate

3272-04

J.T.Baker

Mobile Phase Additives

Ammonium Hydroxide

9721-00

J.T.Baker

Mobile Phase Additives

Acetic Acid

9508-00

J.T.Baker

Mobile Phase Additives

Formic Acid0128-01

J.T.Baker

vwr.com | HPLC application note & product information 29

ACE portfolio specifications
15 PHASES

11 STANDARD COLUMN LENGTHS

C18 | C18-AR | C18-PFP | C18-Amide | CN-ES | SuperC18| SuperPhenyHexyl | AO | C8 | C4 | Phenyl | Amino | CN |C 18-HL | Silica | *20 mm | 30 mm | 35 mm | 50 mm | 75 mm | 100 mm | 125 mm
150 mm | 200 mm | 250 mm | 300 mm
12 STANDARD COLUMN LENGTHS

3 PORE SIZES

0.075 mm | 0.10 mm | 0.30 mm | 0.50 mm | 1.0 mm | 2.1 mm
3.0 mm | 4.0 mm | 4.6mm | 10.0 mm | 21.2 mm | 30.0mm

90A | 100A | 300A
8 PARTICLE SIZESSix fully Porous particles. 1.7 μm | 2 μm | 3 μm | 5 μm | 10 μm |15 μm | and two superficially porous particles. 2.5 μm | 5 μm

PHASE

USP LISTINGFUNCTIONAL GROUPENDCAPPEDPARTICLE SIZE (µm)*

PORE SIZE (Å)

SURFACE AREA (m2/g)

100% AQ
CARBON LOAD (%) PH RANGE compatible

ACE® Traditional Chemistries
C18

L1

Octadecyl

Yes

1.7, 2, 3, 5, 10

100

300

15.5

2–8

-

C18-HL

L1

Octadecyl

Yes

3, 5, 10, 15

90

400

20

2–8

-

C8

L7

Octyl

Yes

2, 3, 5, 10

100

300

9

2–8

-

C4

L26

Butyl

Yes

2, 3, 5, 10

100

300

5.5

2–8

-

Ph

L11

PhenylYes

2, 3, 5, 10

100

300

9.5

2–8

-

CN

L10

Cyano

Yes

2, 3, 5, 10

100

300

5.5

2–7

-

AQ

L1

ProprietaryYes

2, 3, 5, 10

100

300

14

2–8

YES

SIL

L3

UnbondedNo2, 3, 5, 10

100

300

N/A

2–7

-

ACE NH2

L8

Proprietary aminopropyl

Proprietary

1.7, 3, 5

100

300

3.5

2–7

YES

Octadecyl encapsulated
Octadecyl with integral
phenylOctadecyl with integral
PFP

Encapsulated

1.7, 2, 3, 5, 10

90

400

14.8

1.5 – 11.5-

Yes

1.7, 2, 3, 5, 10

100

300

15.5

2–8

YES

Yes

1.7, 2, 3, 5, 10

100

300

14.3

2–8

YES

Polar embedded amide
CN with extended alkyl
spacerYes

1.7, 2, 3, 5, 10

100

300

16.4

2–8

YES

Yes

1.7, 2, 3, 5, 10

100

300

12.6

2–8

YES

2.5

95

130

7

1.5 – 11.0

-

100

5.4
1.5 – 11.0-

ACE® Novel Chemistries
SuperC18L1

C18-AR

L1

C18-PFP

L1

C18-Amide

L1 / L60

CN-ES

L10

Solid Core Technology ACE® Phases
UltraCore

L1

Octadecyl encapsulated

Encapsulated

SuperC18
UltraCore

5
L11SuperPhenylHexyl

Phenyl Hexyl

Encapsulated

Encapsulated

2.5

95

5

130

4.6

100

3.6

Large Molecule Wide Pore ACE® Phases
C18-300

L1

Octadecyl

Yes

3, 5, 10

300

100

9

2–8

-

C8-300

L7

Octyl

Yes

3, 5, 10

300

100

5

2–8

-

C4-300

L26

Butyl

Yes

3, 5, 10

300

100

2.6

2–8

-

CN-300

L10

Cyano

Yes

3, 5, 10

300

100

2.6

2–7

-

Ph-300

L11

PhenylYes

3, 5, 10

300

100

5.3

2–8

-

ACE HILIC-A

L3

Proprietary SIL

No1.7, 3, 5

100

300

N/A

2–7

-

ACE HILIC-B

L8

Proprietary aminopropyl

No1.7, 3, 5

100

300

4

2–7

-

ACE HILIC-N

pending

Proprietary polyhydroxyNo1.7, 3, 5

100

300

7

2–7

-

C18 (2)

L1

Octadecyl

Yes

1.8, 3, 5

95

350

20.3

2–8

-

C8 (2)

L7

Octyl

Yes

1.8, 3, 5

95

350

11.9

2–8

-

Phenyl-Hexyl

L11

Phenyl Hexyl

Yes

1.8, 3, 5

95

350

15.2

2–8

-

Sil

L3

UnbondedNo

1.8, 3, 5

95

350

-

2–8

-

ACE® HILIC Phases

ACE® Generix

30 HPLC application note & product information | vwr.com

J.T.Baker®
LC/MS and HPLC solvents and reagents
Product name

Code

Acetonitrile 4L

9017-88

Methanol 4L

9093-88

Water 4L

4218-88

Hexanes(95% N-Hexane) 4L

9304-88

Acetone 4L

9002-88

Tetrahydrofuran 4L

9440-88

2-Propanol 4L

9095-88

Application

J.T. Baker• BAKER ANALYZED- HPLC
Reagents

J.T. Baker BAKER ANALYZED- LC/MS J.T. Baker BAKER ANALYZED- ULTRA J.T. Baker ULTRA RESI-ANALYZEDReagents
LC/MS Reagents
Reagents

HPLC - converntlonol◆◆◆◆

HPLC - QC/QA◆◆◆◆

HPLC - Research

◆◆◆

◆◆

◆◆◆

LC/MS - QC/QA◆◆

Gos Chromatography(GC}

◆◆◆
◆◆◆

◆

LC/MS - Research

◆◆◆

◆◆

LC/MS - Critical Research

◆◆

◆◆◆

UHPLC - QC/QA

◆◆◆

◆

◆

UHPLC - General research

◆◆◆

◆

◆◆◆

◆

◆◆◆

UHPLC - Critical research◆ Suitable ◆◆ Preffered ◆◆◆ Ideal‒‒ Low backgrounds free of extraneous peaks‒‒ Low UV absorbance in critical ranges assured throughUV absorbance‒‒ Testing at a variety of points and through gradientelution testing‒‒ Fluorescence testing for trace impurities, which would
cause interference‒‒ Function-tested for: assay, water, minimal residue after
evaporation and UV absorbance and fluorescence in
critical ranges‒‒ Innovative packaging options to assure solvent quality to thepoint-of-use‒‒ Lot -to-lot consistency

vwr.com | HPLC application note & product information 31

J.T.Baker®
ULTRA RESI-ANALYZED™ SOLVENTSFor Gas Chromatography, Environmental Extraction
Applications, and EPA Protocols (Water and Soil)
Product

Product Number

Acetone9254

Acetonitrile

9255

Chloroform

9257

Cyclohexane

9258

Ether

9259

Ethyl Acetate

9260

n-Heptane

9338

Hexanes (99% n-hexane)

N168

Hexanes (95% n-hexane)

9262

Methanol

9263

2-Propanol

9334

Toluene

9336

Water

4219

Methylene Chloride

9264

APPLICATION-OPTIMIZED:FUNCTION-TESTED:‒‒ Dual stabilizer systems provide unmatched product formethylene chloride.
‒‒ Optimized distillation processes target removal of reactive
impurities, production of higher assays, and narrowsolvent fronts.
‒‒ Blanketed with inert gas to prevent contamination.

‒‒ Extensively characterized by high resolution capillary GC.
‒‒ Suitability tests on both ECD and FID detectors.
‒‒ Meets EPA requirements for extraction/concentration‒‒ procedures and AOAC requirements for pesticide residue
analysis.
‒‒ Performance-tested to purity levels below the Lower Limit of
Quantitation (LLQ) for trace analyte detection by standard
EPA methods.
‒‒ Meets or exceeds ACS specifications

32 HPLC application note & product information | vwr.com

J.T.Baker®
Solid phase extraction products

GENERAL PRODUCT OVERVIEW TABLE
Description
Standard J.T.Baker® BAKERBOND™ spe 1,
3 and 6ml columns, round-rimmed andearshaped in ultraclean polypropylene
and glass
J.T.Baker® BAKERBOND Speedisk™ 1, 3
and 6 ml columns are configured to run 9
times faster than traditions SPE columns,
operating with smaller solvent volumes
and having higher capacity per milligram
sorbent than conventional SPE columns
50 mm disks that are the correct choice
for samples from 200 ml to 2 L. They areneither cartridge nor membrane. A thin
bed of J.T.Baker® BAKERBOND™ sorbent
micro particles is supported in a laminarstructure to maintain speed and capacityand enhance reproducibility of adsorption.

Attribute

Silica based SPE products

J.T.Baker
BAKERBOND™
spe columns

Reverse phase Normal phase
Ion exchange Adsorption
Drug of abuse

J.T.Baker®
BAKERBOND
Speedisk™
Columns

Reverse phase Normal phase
Ion exchange Adsorption
Drug of abuse

J.T.Baker®
BAKERBOND
Speedisk™
Extraction Disks

Extraction disks for manual
extraction stations and forautomated extractors®

J.T.BAKER® BAKERBOND™ SPE SILICA-BASED COLUMNS
Description

General application

Octadecyl (C18)

Non-ionic, non-polar to moderately polar analytes

PolarPlus Octadecyl (C18)

Non-ionic, non-polar to moderately polar analytes

Octyl (C8)Non-ionic, non-polar to moderately polar analytes

Phenyl (C6H5)

Polar from non-polar/polar solvents using hydrogenbonding like mechanisms

Cyano (CN)Non-ionic, non-polar to moderately polar analytes

Diol (COHCOH)

Non-ionic, polar analytes

Amino (NH2)

Lipids (fatty acids, cholesterol)

Quaternary Amine (N+)

Ionic, acidic analytes

Aromatic Sulfonic Acid (ArSO2OH)

Ionic, acidic analytes

Carboxylic Acid (COOH)

Ionic, acidic analytes

Florisil (Mg2SiO3)

Low to moderately polar analytes from
nonaqueous solutions

Silica Gel (SiOH)

Polar analytes from non-polar solvents likehydrocarbons and less polar esters and ethers

Alumina Neutral

Weakly or moderately polar compounds

Narc-2 (Cocaine, BEC)

Hydrophobic/basic analytes(Cocaine, Benzoylecgonine)

Wide Pore Butyl (C4)

Small peptides, separations where C18 gives excessive
retention or poor recovery

Wide Pore CBX

Weak bases such as purines, pyrimidines, vitamin B6,
cyclic hydroxyamines

vwr.com | HPLC application note & product information 33

VWR Chemicals BDH
Lab reagents for quality control and research
BDH LC-MS SOLVENT OFFER‒‒ High purity‒‒ Various product list‒‒ Competitive Price‒‒ Good reference at Europe

HiPerSolv CHROMANORM® LC-MS and ULTRA LC-MS solvents for LC-MS and ULC-MS applications
Product Part No.

Acetonitrile

Acetonitrile ULTRA

1L

83640.290

83642.290

2.5L

83640.320

83642.320

Ethyl acetate
85481.320

Methanol

Methanol ULTRA

2-Propanol

THF

Water

83638.290

85800.290

84881.290

84882.290

83645.290

83638.32085800.320

84881.320

84882.320

83645.320

HiPerSolv CHROMANORM® LC-MS and ULTRA LC-MS solvents for LC-MS and ULC-MS applications
Product Part No.

Acetic acid 99%

Ammonium acetate

Ammonium formate

Formic acid

Triethylamine

Trifluoroacetic

100ml

84874.180

84885.180

84884.180

84865.180

84883.180

84868.180

500ml

84874.260

84885.260

84884.260

84865.260

84883.260

84868.260

1L84874.290

84865.290

84868.290

HiPerSolv CHROMANORM® LC-MS grade mixes for HPLC
Product Part No.

Acetonitrile with 0,1%
acetic acid

Water with 0,1%
acetic acid

Acetonitrile with 0,1%
formic acid

Water with 0,1%
formic acid

Acetonitrile with
0,1% TFA

Methanol with
0,1% TFA

Water with 0,1% TFA

1L84872.290

84873.290

84866.290

84867.290

84869.290

84870.290

84871.290

2.5L

84872.320

84873.320

84866.320

84867.320

84869.320

84870.320

84871.320

BDH HAS EVERY ITEMS FOR QC‒‒ ICP/ICP_MS Standard‒‒ Karlfish‒‒ GC solvent /‒‒ Media for environmental monitoring‒‒ Karl fisher‒‒ Kjeldahl catalysts‒‒ ETC34 HPLC application note & product information | vwr.com

RANKEM
HPLC solvents – enable better detection andseparation of chemical compounds
HPLC TECHNIQUES GIVE BEST RESULTS FOR
IMPURITIES PROFILE AND PURITY VERIFICATION‒‒ Controlled acidity and alkalinity‒‒ Low in moisture for better performance in normal phasecromatography‒‒ Ensure high reproducibility and accuracy in results‒‒ Minimum baseline noise in gradient analysis‒‒ Filtered through 0.2 Micron filers to enhance longevityof the column‒‒ Accurate peak quantification‒‒ High resolution and detection of trace impurities‒‒ Processed with rigorous quality checks to ensure batch tobatch consistency‒‒ Available in small and replaceable large packs‒‒ Free from uv absorbing impurities to ensure there are nointerfering peaks
RANKEM HPLC SOLVENTS ARE IDEALLY SUITABLE FOR‒‒ Sample preparation‒‒ Method development‒‒ Preparation chromatography‒‒ Analytical application

Detector

Condition

Code

Product Name

PDA

A2104

Acetonitrile preparative HPLC-2.5L

HPLC Column

C-18, 5micron, 250mm x4.6mm

M0170

Methanol LR-2.5 L

Mode

Gradient

P0690

Propanol-2-Ol( Iso-propyl alcohol) LR

Mobile phase

Water HPLC/Acetonitrile HPLC

A0160

Acetone LR-2.5 L

Method0-100% ACN over 24min (V/V)

H0100

Hydrochloric acid LR-2.5 LIT

Wavelength

205nm and 254nm

W0020

Water HPLC 1L

Flow Rate

1.8ml/min

S0550

Sulphuric acid LR-2.5 LIT

N0090

Nitric acid LR-2.5 LIT

M0270

Methanol HPLC-1 L

vwr.com | HPLC application note & product information 35

VWR Consumable
SYRINGE FILTERS‒‒ Available with either PP, PTFE or nylon membrane.
Membranes are available in either 0,2 or 0,45 μm pore sizewith the choice often made on analytical column packing
size. If the column packing size is larger than 3 μm then use
0,45 μm and if 3μm or smaller then use 0,2 μm.
‒‒ Suitable for HPLC: Low levels of UV-detectable extractables

Pore size (μm)

Ø (mm)

SterilePk

Cat.No.

Cellulose acetate membrane
0.2 **

25+

50

VWRI514-0061

0.2 **

25-

100

VWRI514-0060

0.45

25

+

50

VWRI514-0063

0.45

25

-

100

VWRI514-0062
VWRI514-0073

PES membrane
0.2

25

+

50

0.2

25

-

100

VWRI514-0072

0.45

25

+

50

VWRI514-0075

0.45

13

-

100

VWRI514-0074

Pore size (μm)

Ø (mm)

SterilePk

Cat.No.

0.2

25

-

100

VWRI514-0064

0.45

25

-

100

VWRI514-0065

0.2

13

-

100

VWRI514-0068

0.2

25

-

100

VWRI514-0070

0.45

13

-

100

VWRI514-0069

0.45

25

-

100

VWRI514-0071

0.2

25

-

100

VWRI514-0066

0.45

25

-

100

VWRI514-0065

PP membrane

PTFE membrane

Nylon membrane

36 HPLC application note & product information | vwr.com

VWR Consumable
VWR ALL PP SYRINGES‒‒ Luer tip type‒‒ Luer lock type

SINGLE-USE SYRINGES
Disposable, two-piece, without needle, PP barrel and PE
piston, latex- and silicone oil-free. Syringes are also available
with especially designed oral tip, which prevents accidental
Luer or hypodermic connection, in volumes of 5 or 10 ml.
Incompatible with injection needles. Please enquire for details.
‒‒ Defined position of the plunger at volume "0" to feel whenplunger is completely inserted‒‒ No dead space for 1 ml insulin and tuberculin syringe‒‒ Extended graduation for wider range of applications‒‒ Positive safety stop to prevent accidental spills
Packaging: Sterile versions are packed in individual blister strips.
Non sterile versions are bulk packed.

Description

Capacity

Pk

Cat.No.

Luer tip type
Single-use syringe, Luer tip

1ml

100

VWRI613-2001

Single-use syringe, Luer tip

2ml

100

VWRI613-2003
VWRI613-2005

Single-use syringe, Luer tip

5ml

100

Single-use syringe, Luer tip

10ml

100

VWRI613-2007

Single-use syringe, Luer tip

20ml

100

VWRI613-2009

Single-use syringe, Luer tip

30ml

50

VWRI613-2033

Single-use syringe, Luer tip

50ml

30

VWRI613-2036

Luer lock type
Single-use syringe, Luer lock tip

2ml

100

VWRI613-2004

Single-use syringe, Luer lock tip

5ml

100

VWRI613-2006
VWRI613-2008

Single-use syringe, Luer lock tip

10ml

100

Single-use syringe, Luer lock tip

20ml

100

VWRI613-2010

Single-use syringe, Luer lock tip

30ml

50

VWRI613-2035

Single-use syringe, Luer lock tip

50ml

30

VWRI613-2038

vwr.com | HPLC application note & product information 37

VWR Consumable
VWR disposable gloves

LATEX, NON STERILE, POWDER-FREE,TEXTURED FINGERTIPS

THIN NITRILE, NON STERILE,
POWDER-FREE, TEXTURED FINGERSNITRILE, NON STERILE, POWDER-FREE,TEXTURED FINISH

Single-use, ambidextrous gloves madefrom natural latex. Suitable for foodhandling, laboratory and industrial
applications with complex risks and for
medical applications.

Thin ambidextrous gloves are suitablefor use in medical applications, foodprocessing and for general applications
in non hazardous areas.

For laboratory use in pharmaceutical,
industrial and medical applications andin the food industry.

Very thin : Provide excellent elasticity
for good wearing comfort.

Description

Highly resistant to chemicalsolvents and fats.

Size

pk

Cat.No.

GLOVE TEXTURED POLYMER COATED PF LATEX

XS

100

VWRA112-5397

GLOVE TEXTURED POLYMER COATED PF LATEXS

100

VWRA112-5398

Latex powder-free gloves

GLOVE TEXTURED POLYMER COATED PF LATEX

M

100

VWRA112-5399

GLOVE TEXTURED POLYMER COATED PF LATEX

L

100

VWRA112-5440

GLOVE TEXTURED POLYMER COATED PF LATEX

XL

90

VWRA112-5441

Nitrile gloves, NITRILE EXTRA LIGHT

XS

200

VWRI112-4193

Nitrile gloves, NITRILE EXTRA LIGHT

S200

VWRI112-4194

Thin nitrile powder-free gloves

Nitrile gloves, NITRILE EXTRA LIGHT

M

200

VWRI112-4195

Nitrile gloves, NITRILE EXTRA LIGHT

L200

VWRI112-4196

Nitrile gloves, NITRILE EXTRA LIGHT

XL

180

VWRI112-4197

GLOVE NITRILE AMBI POWDER-FREE

S100

VWRI112-2371

GLOVE NITRILE AMBI POWDER-FREE

M

100

VWRI112-2372

Strong chemical resistant nitrile powder-free gloves

GLOVE NITRILE AMBI POWDER-FREE

L

100

VWRI112-2373

GLOVE NITRILE AMBI POWDER-FREE

XL

90

VWRI112-3101

38 HPLC application note & product information | vwr.com

VWR Equipment
BOTTLE-TOP DISPENSERVARIABLE VOLUME, AUTOCLAVABLE.
Fine adjustment for exact, reproducible dispensing. All wettedparts are chemically resistant and autoclavable at +121 °C.
Easily removable PTFE piston for smooth action and easycleaning. Borosilicate glass barrel protected with a transparent
PP sleeve can be easily removed and cleaned. Easy priming
and minimum waste with no leakage back into the reservoir

Description

Range

Accuracy

VWR BOTTLE TOP DISPENSER 1-10ML ZIPPETTE

1~10ml±0.3%

Cat.No.
VWRI612-4178

VWR BOTTLE TOP DISPENSER 3-30ML ZIPPETTE

3~30ml

±0.3%

VWRI612-4179

VWR BOTTLE TOP DISPENSER 5-50ML ZIPPETTE

5~50ml

±0.3%

VWRI612-4180

VWR BURETTE DIGITAL 50ML DIGITRATE PRO

0~50ml

±0.2%

VWRI613-5287

We can handle a lot of
equipment and instruments
INSTRUMENTS AND EQUIPMENT'S
The VWR Collection offers an extensive selection of laboratory
Instruments & Equipment's ranging from bench top to higher
end instruments
PORTFOLIO OVERVIEW‒‒ Refrigerators, freezers & ULT's‒‒ Ovens‒‒ Incubators and heating blocks‒‒ Glassware washers‒‒ Benchtop and micro centrifuges‒‒ Vortex mixers‒‒ Ultrasonic cleaners‒‒ Shakers‒‒ Microscopes‒‒ Ph meters conductivity meters & electrodes‒‒ Balances and moisture balances‒‒ Air samplers‒‒ Hotplates and stirrers‒‒ Spector photometers‒‒ Bath chillers circulators and thermostats pumps‒‒ Homogenizers‒‒ Autoclaves

vwr.com | HPLC application note & product information 39

VWR.COMVWR International
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02/2020-SM-KM

Your contact person
HPLC application note &product information