IHC

Immunohistochemistry

Polymer Detection System for Human-Histofine®

Advantages of-Histofine®

in comparison with conventional streptavidin-biotin systemwaMoushinnt
ging-Histofine® Simple Stain

Conventional
Streptavidin-biotin System(RT 30min.)Protein Blocking(RT 10min.)Primary antibody(RT or 4℃)

ginshwa

gin

shwa

Biotinylated
Streptavidin-enzyme
Secondary antibody conjugate(RT 10min.)ginshwa

ginshwa

M
ount
ing

(RT or 4℃)

Counterstaining

Primary antibodyCounterstaining

washing

Simplified
staining
steps!

wa
shing-Histofine® Simple Stain(RT 5-10 min.)-Histofine® Simple Stain

Highsensitivity!

Primary antibody
Tissue section

AntigenConventional Streptavidin-biotin System

Primary antibody
Tissue section

Antigen-Histofine® Simple Stain

Unaffected byendogenous
biotin in thetissue!
:-Histofine® Simple Stain: Biotin

Conventional Streptavidin-biotin System

Backgroundstaining: Biotinylated secondary antibody: Streptavidin-enzyme conjugate: BiotinDetection system for Human tissue sections-Histofine® Simple Stain MAX PO
This product adopted the UIP (Universal Immuno-enzyme Polymer) method, Nichirei Bio’s patented technology.

Feature-Histofine® Simple Stain MAX PO is a detection reagent designed specifically to allow immunohistochemical
staining on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by
combining amino acid polymers with multiple molecules of peroxidase (PO) and secondary antibody which is
reduced to Fab’ fragment. To eliminate background staining, solid-phase absorption of secondary antibody is
carried out with human serum.

Principle & Procedure
Fab' fragment ofsecondary antibody-Histofine®

Simple Stain MAX PO
Peroxidase

Amino-acidpolymer

ngniitagrsetintnnuouM
Co

Human tissue
sectionAntigen
30 min.
Add primary antibody.

Add polymer.

Add substrate solution.

Advantages
1. Simplified staining steps.
2. High sensitivity.
3. Unaffected by endogenous biotin in the tissue.
4. Ready to use.

Staining Results■ Mouse anti-Progesteron
Receptor antibody (clone:1A6)■ Rabbit anti-S-100 protein
antibody■Mouse anti-Estrogen Receptor
antibody (clone: 1D5)

Human breast cancer (treated with hightemperature epitope unmasking method) stainedwith-Histofine® Simple Stain MAX PO(M) andDAB chromogen.
Note nuclear staining of breast cancer cells.

Human colon stained with-Histofine® Simple
Stain MAX PO(R) and DAB chromogen.
Note cytoplasmic staining of nerve cells.

Human breast cancer (treated with hightemperature epitope unmasking method) stainedwith -Histofine® Simple Stain MAX PO (MULTI)
and DAB chromogen.
Note nuclear staining of breast cancer cells.

Detection system for Human tissue sections-Histofine® Simple Stain AP
This product adopted the UIP (Universal Immuno-enzyme Polymer) method, Nichirei Bio’s patented technology.

Feature-Histofine® Simple Stain AP is a detection reagent designed specifically to allow immunohistochemical staining
on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by combining
amino acid polymer with alkaline phosphatase (AP) and secondary antibody which is reduced to Fab’ fragment. To
eliminate background staining, solid-phase absorption of secondary antibody is carried out with human serum.

Principle & Procedure
Fab' fragment ofsecondary antibody-Histofine®

Simple Stain AP
Alkaline
phosphatase

Amino-acidpolymer

Human tissue
sectionAntigen
30 min.

Add primary antibody.

Add polymer.

Add substrate solution.

ngniitagrsinettnunou
M
Co

Advantages
1. Simplified staining steps.
2. High sensitivity.
3. Unaffected by endogenous biotin in the tissue.
4. Ready to use.

Staining Results■Mouse anti-Muscle Actin antibody(clone:HHF35)■Rabbit anti-S-100 protein antibody
Human stomach stained with -Histofine®
Simple Stain AP (M) and New fuchsin
chromogen.
Note intense staining of smooth muscle in
the walls of blood vessel and muscularis
mucosae.

Human colon stained with -Histofine®
Simple Stain AP (R) and New fuchsin
chromogen.
Note cyroplasmic staining of nerve cells
scattered in smooth muscle andAuerbach’s plexus.

Product List

Liquid. Ready to use.
Mark in EU

For Human Tissue Sections
1705001500
170500
1500
170
500
1500
170
500
1705001705001705001 x 17ml
3 x 17ml
9 x 17ml
1 x 17ml
3 x 17ml
9 x 17ml
1 x 17ml
3 x 17ml
9 x 17ml
1 x 17ml
3 x 17ml
1 x 17ml
3 x 17ml
1 x 17ml
3 x 17ml
1 x 17ml
3 x 17ml

414151F
Mouse and rabbit
414152F
primary antibodies
414154F
414131F
Mouse
414132F primary antibody
414134F
414141F
Rabbit414142F
primary antibody
414144F
414161F Goat
414162F primary antibody
414261F Mouse and rabbit
414262F primary antibodies
414241F Mouse
414242F primary antibody
414251F Rabbit
414252F primary antibody

500
1500

3 x 3ml
3 x 9ml

415192F
Peroxidase
415194F-Histofine® Simple Stain AEC Solution

500
1500

3 x 17ml
9 x 17ml

415182FPeroxidase
415184F-Histofine® New Fuchsin Substrate kit

2000

3 x 6ml,1 x 12ml

Alkaline
415161F phosphatase-Histofine® Simple Stain MAX PO (MULTI)-Histofine® Simple Stain MAX PO (M)-Histofine® Simple Stain MAX PO (R)-Histofine® Simple Stain MAX PO (G)-Histofine® Simple Stain AP (MULTI)-Histofine® Simple Stain AP (M)-Histofine® Simple Stain AP (R)
Enzyme Substrate Systems-Histofine® DAB-3S kit

Reagent A : DAB solution concentrate
Reagent B : Enhancer solution concentrate
Reagent C : Hydrogen peroxide solution concentrate

Reagent A : New Fuchsin solution concentrate
Reagent B : Activating reagent concentrate
Reagent C : Substrate buffer concentrate
Reagent D : Substrate solution concentrate-Histofine® series application on IHC staining
TissueEnzymeHRP

Primary antibody

Detection systems

Mouse orRabbit ab

Simple Stain
MAX PO (MULTI)Mouse ab

Simple Stain
MAX PO (M)Rabbit ab

Simple Stain
MAX PO (R)

Goat ab

Simple Stain
MAX PO (G)Mouse orRabbit ab

Simple Stain
AP (MULTI)Mouse ab

Simple Stain
AP (M)

Rabbit ab

Simple Stain
AP (R)

Human TissueAP6-19-20, TSUKIJI, CHUO-KU,TOKYO, 104-8402, JAPAN
PHONE: 81-3-3248-2207
FAX: 81-3-3248-2243
http://www.nichirei.co.jp/bio/english

2011.01