J.T.Baker® BAKERBOND®
PROchievA™ recombinant
protein A affinity
chromatography resin
Enabled by a new, proprietary protein A derived ligand, J.T.Baker®
BAKERBOND® PROchievA™ provides best-in-class purification
performance for monoclonal antibodies, Fc-fusion proteins, andIgG antibody type molecules.

Designed and manufactured by Avantor to the highstandards established by our J.T.Baker® brand, theBAKERBOND® PROchievA™ resin is designed for highperformance in the critical affinity chromatography
step of mAbs and Fc-fusion protein manufacturing. Its
new ligand provides an independent chromatography
resin supply alternative for protein A resins, delivered in a
non-flammable storage solution through Avantor’s global
supply chain.
Used with Avantor’s proven J.T.Baker® family of process
chromatography buffers and additives, the BAKERBOND®
PROchievA™ resin can provide biopharma operations withgreater efficiencies and higher purity profiles within theaffinity chromatography step.

Features

Higher purity levels– Proprietary ligand developed exclusively by Avantor withdemonstrated first-class dynamic binding capacity formAbs and improved protein purification capability in othercomplex molecules– Standard agarose backbone for ease-of-use and supply
reliability– Traditional particle size allows for use of established column
packing procedures and operating protocols– Delivery in a non-hazardous, non-flammable buffer solution
to eliminate burdensome handling requirements

As biologic molecules become more complex, so will thedemands on downstream processing. In several applications
the BAKERBOND® PROchievA™ resin has been shown to deliver
higher levels of protein purity, thus reducing the burden on
subsequent purification operations. Several examples areshown in the emerging market for Fc-fusion proteins.

Dynamic binding capacity is a primary driver to processintensification as it directly drives resin and buffer consumption,
cycle time, and capital investment. BAKERBOND® PROchievA™
resin provides equivalent dynamic binding capacities as
the best-in-class protein A resins on the market today andsignificantly higher performance relative to the most commonlyused protein A resins on both the process development lab andmanufacturing scale.
The high DBC and high column linear velocity offeredby BAKERBOND® PROchievA™ affinity chromatography
resins gives biopharma operations the ability to intensify
downstream throughput while generating higher puritymAb products.

90
Target Protein Purity (%)

Proven dynamic binding capacity

100
80
70
60
50
4030
20
100
Fc-Fusion Protein 1

Fc-Fusion Protein 2

Avantor PROchievACompetitor A

Testing performed on two different Fc-fusion proteins.

Alkaline stability

In addition, the porous 75µm particles and inherent lack of fines
enable high flow rates to be used during purification, resulting
in higher efficiency in downstream processing.

The proprietary ligand in the BAKERBOND® PROchievA™ resin
has been engineered to be stable under alkaline conditions,so standard clean-in-place (CIP) procedures using sodiumhydroxide can be performed. As a result, the BAKERBOND®
PROchievA™ chromatography resin will retain its high dynamicbinding capacity through several purification cycles, providing
cost-effective means for performing multiple purifications.

7060
50403020
100012345678910Residence Time (minutes)
Competitor ACompetitor B

Competitor C

Competitor D

Competitor E

Avantor PROchievA

Relative Capacity at 10% Breakthrough(percent)Dynamic Binding Capacity at 10%Breakthrough (mg/mL)

120100806040200020406080100Cycle
0.1 N NaOH - 60 minute contact time

Using purified human IgG1.

2vwr.com | J.T.Baker® BAKERBOND® PROchievA™ protein A resin

0.5 N NaOH - 15 minute contact time

Each cycle represents equilibration, load, wash, elute and CIP with 3CV NaOH. Alkaline stability
evaluation was performed at 4⁰ C.

Convenient packaging

Process and applications support

The BAKERBOND® PROchievA™ resin comes conveniently
packaged in a non-hazardous/non-flammable storage buffer,eliminating many of burdensome shipping, handling, andstorage requirements common among protein A resins.

Avantor has deep expertise in process chromatography
optimization and can work with you to help ensure that theBAKERBOND® PROchievA™ affinity chromatography resin delivers
the improved performance you need in your protein A purification
step. Technical support from our scientists and application
specialists is available from our multiple global research andinnovation centers.

Form

Pre-Packed LabColumns

ResinSize
1 mL column(box of 1)
1 mL column(box of 5)
5 mL column(box of 1)
5 mL column(box of 5)
25 mL
100 mL
500 mL
1L
5L

AvantorPart Number

VWR North
American Cat. No.

VWR EU/AMEA
Cat. No.

C789-11

JTC789-11

C789-11

C789-07

JTC789-07

C789-07

C789-18

JTC789-18

C789-18

C789-25
7899-01
7899-027899-037899-04
7899-05

JTC789-25
JT7899-01
JT7899-02
JT7899-03
JT7899-04
JT7899-05

C789-25
7899-01
7899-027899-037899-047899-05

Key application information
Functionality

IgG antibody and Fc-fusion protein capture

Functional Group

Recombinant protein affinity ligand for IgG antibodies

Dynamic Binding Capacity>65mg hIgG/mL at 8 minutes residence time1

Average Particle Size

70–80 µm

Binding Buffers

HEPES, PBS, Tris

Binding pH Range

7.0–8.0

Elution pH Range

3.0–4.4

Cleaning pH Range

11.0-14.0

Shipping Stability

200 mM sodium acetate, 2% alcohol,
2% benzyl alcohol, 2–8°C

1

Dynamic binding capacity was determined at 10% breakthrough.

Contact your VWR sales representative to learn more about
J.T.Baker® BAKERBOND® PROchievA™ chromatography resin orother BAKERBOND® resins.

J.T.Baker® BAKERBOND® PROchievA™ protein A resin | vwr.com

3Setting science inmotion to create
a better world
From breakthrough discovery to agile delivery, we offer an extensive
portfolio of mission-critical products, services, and solutions. We area trusted global partner to customers in the life sciences, advancedtechnologies, and applied materials industries.

VWR, part of Avantor provides an integrated, seamlesspurchasing experience optimized for your success.
Learn how the new Avantor is moving forward atavantorsciences.com©2020 Avantor ®, Inc. All rights reserved.

Prices, product, and/or services details are current when published and subject to change without notice. | Certain products or services may be limited by country, federal, state, provincial, or local
regulations. | VWR, part of Avantor, makes no claims or warranties concerning sustainable/green products. Any claims concerning sustainable/green products are the sole claims of the manufacturer andnot those of VWR International, LLC and/or Avantor, Inc. or affiliates. Offers valid in countries listed above, void where prohibited by law or company policy, while supplies last. | Trademarks are owned byAvantor, Inc. or its affiliates, unless otherwise noted. | Visit vwr.com to view our privacy policy, trademark owners, and additional disclaimers. © 2020 Avantor, Inc. All rights reserved.

ZPROAVTR26819-EN

26819-KS-WH

PB19019_NA