Operating Instruction Centrifuge LC 6

Instruction ManualCentrifuge LC 6
with horizontal rotor 6 x
SARSTEDT-No.: 90.184.500

V. 2.1.2 / 01.2009

Operating Instruction Centrifuge LC 6

Table of contents
Page E-

1. Device data ........................................................................................................................ 3
2. Manufacturer's address ...................................................................................................... 3
3. Important notes................................................................................................................... 3
Explanation of symbols.................................................................................................................4
Device-specific safety notes .........................................................................................................4
Responsibilities of the owner........................................................................................................5
Responsibilities of operating personnel ........................................................................................5

4. Other information
Guarantee and liability..................................................................................................................6
Shutdown / Disposal.....................................................................................................................6

5. Description of the centrifuge............................................................................................... 6
6. Transport, Installation and Connection ............................................................................... 7
Delivery Schedule ........................................................................................................................7
Unpacking ....................................................................................................................................7
Technical Data .............................................................................................................................8
Installation....................................................................................................................................8
Rotor Removal and Installation.....................................................................................................9
Commencing Operation................................................................................................................9

7. Operation.......................................................................................................................... 10
Control Elements........................................................................................................................10
Setting the time ..........................................................................................................................10
Counter ......................................................................................................................................11
Access to the Rotor Chamber during a Power Failure ................................................................11
Loading the Rotor.......................................................................................................................11
Centrifuging................................................................................................................................11

8. Maintenance and servicing
Service address .........................................................................................................................12
Cleaning.....................................................................................................................................13
Regular Maintenance .................................................................................................................13
Index ..........................................................................................................................................13
contamination questionnaire.......................................................................................................14page E-2

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Operating Instruction Centrifuge LC 6

1. Device data
(to be completed by customer during installation)
Type:______________________________________________________________________________________________________

Serial no.:___________________________________________________

Place of installation:___________________________________________________

Date of installation:___________________________________________________

Inventory no.:___________________________________________________

2. Manufacturer's address
SARSTEDT AG & Co.
Postfach 1220
D-51582 Nümbrecht
Phone: +49 (0) 22 93 / 305 0
Fax:+49 (0) 22 93 / 305 282
E-Mail: info@sarstedt.com

3. Important notes
Read the safety instructions in this manual
before putting the device into operation!
A sound knowledge of the contents of these operating instructions is a basic
requirement to ensure correct use and trouble-free operation of the device.
Please keep these instructions in a safe place for future reference.
Copyright
The copyright for these operating instructions lies with SARSTEDT-AG & Co. The instructions are
only intended for use by operating personnel and for the purchaser of the device.
Reproduction or distribution of this publication, in whole or in part, is not permitted without the writtenconsent of SARSTEDT AG & Co.
All rights reserved. Infringements can be prosecuted.
Sarmix and Sedivette are registered trade names of SARSTEDT AG & Co.
Technical modification reserved.
Nümbrecht, January 2009
SARSTEDT AG & Co.

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Operating Instruction Centrifuge LC 6
Explanation of symbols
These operating instructions contain important symbols to indicate dangers and possible operating
errors.
The ”Caution“ symbol means that in this section:• Important information is given.
• An immediate or possible risk of danger to life and limb of persons is
described.
• A note on dangerous situations is given.
Non-observance of these symbols can lead to minor, serious, or evenlife-threatening injuries as well as to material damage.

Warning of hazardous electrical voltage!

Warning against harmful or irritating substances!
• Failure to observe this symbol can result in severe harm to health.
• Always comply with the applicable accident prevention regulations (APR)
when handling these substances.
This “Info” symbol refers to notes regarding proper use of the device or to general,
explanatory information. Non-observance of these notes or information can lead to thedevice being operated incorrectly or even becoming damaged.
Additional symbols contain notes on the topic displayed in the symbol, for example
accident prevention regulations, supply voltage, maintenance, or disposal.

Device-specific safety notes
This device has been built according to the latest state of the art and the accepted
principles of safety engineering.
All relevant safety information and guidelines, as well as regulations for safety at workand accident prevention regulations for laboratory use must be observed.
Nonetheless, danger can arise for the user or third parties during operation, as well as
damage to the device or other material assets.
The device may only be used:• for the intended purpose• in technically safe and perfect condition.
• in adherence with the current accident prevention regulations.
• for the separation of materials of varying density or particle size suspended ina fluid.
• with a maximum sample density of 1.15 g/cm3.
The device may not be used:• in explosion-hazarded areas.
• with flammable or explosive materials or substances that may precipitatestrong reactions with one another.
• for centrifugation of toxic or radioactive materials and pathogenic microorganisms without appropriate safety systems.

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Operating Instruction Centrifuge LC 6
During the warranty period, the device may only be repaired by SARSTEDT AG & Co.
or by persons authorized by SARSTEDT AG & Co.
In case of improper use or repair, the warranty will be void.
The device is a piece of laboratory equipment and complies with the international
standard EN 61010 (safety requirements for laboratory equipment).
The device is operated with a mains voltage of 230 V ~, ± 10 %, 50 Hz. Therefore,
the following notes must be observed:• No water or other liquids may be permitted to enter the device.
• The device may only be connected to the mains supply by means of a suitable
power cord supplied.
• Before operating the device, check the power cord for signs of damage.
• Damaged power cord must not be used under any circumstances.
• The device must only be connected to the mains voltage specified on the typelabel.
• A damaged device must not be operated.
• Never open housing parts that are fixed with screws.
• Remove the plug from the wall outlet, if the device is not used for a prolonged
period of time or if it is to be cleaned.
The device must only be used with the accessories described in these operating
instructions.

Responsibilities of the owner
The device owner is responsible for ensuring that only such persons are allowed to operate thedevice, who have read these instructions and understand them. This should be confirmed by the
person's signature.
Moreover, the observance of safety regulations by personnel should be checked at regular intervals.

Responsibilities of operating personnel
Before working with the device for the first time, the operator must:• read these operating instructions.
• observe the applicable rules and the regulations for accident prevention.

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Operating Instruction Centrifuge LC 6

4. Other information
Guarantee and liability
The “Delivery and Payment Conditions” of SARSTEDT AG & Co. always apply. These are printed on
the reverse side of the invoice.
Guarantee and liability claims are excluded, if they are based on one or more of the following causes:•
use of the device for other than the intended purpose.
•
improper installation, commissioning, operation, or maintenance of the device.
•
operation of the device with defective safety fittings or incorrectly attached or non-functionalsafety or protective equipment.
•
non-observance of the notes in these operating instructions regarding transport, storage,
installation, commissioning, operation, maintenance, setting up, or disposal.
•
unauthorized modifications to the device.
•catastrophic failure due to external cause and/or force majeure.
•incorrect repairs.

Shutdown / Disposal••

If the device is to be scrapped, it must be handled and disposed of in accordancewith the applicable laws and regulations.
All substances used in connection with the device must be handled and disposedof in accordance with the applicable laws and regulations.

These operating instructions consist of the following materials:
Back binder and protective cover are of PVC – the rest is paper.
Remove the paper by tearing off the back binder.

5. Description of the centrifuge
The centrifuge LC 6 is a table-top centrifuge with horizontal rotor designed for continuous operation.
The horizontal rotor contained in the standard delivery schedule and the tube holders canaccommodate up to 6 specimen tubes. The special horizontal rotor facilitates a fast and simple
specimen charge and complete horizontal separation.
A lid safety switch prevents the centrifuge from operating when the lid is open. The yellow “LATCH”
indicator at the front of the centrifuge lights up as soon as the lid has been correctly locked. In
addition to the lid safety switch, the LC 6 is equipped with a “0 rpm” lid locking system. This safetysystem keeps the lid closed at all times (even in the event of a power failure) and ensures that the lid
can be unlocked only when the rotor has come to a standstill.

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Operating Instruction Centrifuge LC 6

6. Transport, Installation and Connection
Delivery ScheduleStandard delivery schedule
1
6
6
6
1
1

x
x
x
x
x
x

Centrifuge LC 6 with horizontal rotor 6 x, SARSTEDT-No. 90.184.500
tube holder, 100 mm
tube holder, 75 mm
Cap for tube holder
power cord
operating instruction

Accessories
92.184.554 0.25 inch rubber adapter
92.184.553 1.0 inch rubber adapter(recommended for glass and flat bottom tubes)(recommended for glass and flat bottom tubes)

If you have any questions concerning other SARSTEDT products or the SARSTEDT product range,
please contact:
SARSTEDT AG & Co.
Postfach 1220
D-51582 Nümbrecht
Telefon:Telefax:+49 (0) 22 93 / 305 0+49 (0) 22 93 / 305 282

or contact your local SARSTEDT sales office.

Unpacking••••

Check that the packaging shows no signs of damage.
In case of transport damage, please contact the shipping agent immediately.
Observe the notification periods of the different freight carriers (railway, mail,parcel service, trucking company, etc.). It can be as short as 24 hours.
Check the scope of delivery for completeness.
Defects and damage must be notified immediately to SARSTEDT AG & Co.

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Operating Instruction Centrifuge LC 6
Technical Data
Machine data:
Apparatus:
Rotor:
Tube holder:
Order number:Centrifuge motor:
Kinetic energy:
Timer:

Centrifuge LC 6
horizontal rotor 6 x
6 pieces
SARSTEDT No. 90.184.500

Speed:
RCF: with tube holder 100 mm
RCF: with tube holder 75 mm
Time preset:

HP brushless induction motor< 200 Nmelectronic,
continuous operation or 1-30 minutes +/- 1 %max. 3,800+/- 100 min-1
max. 2,000 x g+/- 100 x g
max. 1,650 x g+/- 100 x g
15 minPower supply
Electrical connection:
Power consumption:
Fusing:230 V ∼, ± 10 %, 50 Hz
200 watts
4.0 A, re-startable

Ambient conditions
Admissible ambient temperature:
Maximum relative humidity:+5 °C to +40 °C
80 %, non-condensing

Dimensions
Width x depth x height:
Weight:200 x 280 x 340 mm
approx. 10.5 kg

Loading of the rotor:
Max. tube size
17 mm x 100 mm
17 mm x 75 mm

number of tubes
6
6Installation
•
•
•
•
•
•
•
•

Position the centrifuge on a firm, vibration-free and flat surface.
Maintain a safety zone around the centrifuge of at least 30 cm.
During centrifuging, no person or dangerous substance must come within thesafety zone.
During operation, no contact must occur with other equipment or objects.
Do not place vibration-sensitive objects or samples on the installation surface.
There must be sufficient space above the installation surface to open thecentrifuge lid.
Ensure that there is adequate air circulation to prevent the samples and thecentrifuge from overheating.
The installation location must always be well ventilated.

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Operating Instruction Centrifuge LC 6
Rotor Removal and Installation
•
•
•
•

•

Open the lid lock using the“OPEN/(EMERGENCY STOP)“ button.
Pull out the mains plug.
Remove the tube holder.
Losen and remove the rotor nut in the centreof the rotor using a 13 mm
wrench(open counterclockwise direction).
Pull off the rotor in an upwards direction.

The rotor is refitted in the reverse order.

Ensure that the rotor is seated firmly on the axle after fitting
and is correctly screwed on.

Commencing Operation
Caution before first use!
• Check that the actual supply voltage corresponds with the stated supply voltage.
• Comply with the permitted ambient conditions for the machine.
• In the cold season, after delivery and unpacking, wait approx. 1 hour until thecentrifuge has reached ambient temperature (18 °C to 23 °C).
• The control elements are shown in Section 7.
For safety reasons, the locking system is always activated.
• The user can switch it off for approx. 15 seconds by pressing the“OPEN/(EMERGENCY STOP)“ button briefly. This is possible only with thecentrifuge switched on and the rotor at a standstill.
• At the end of a centrifugation, the lid is automatically unlocked for approx. 60
seconds.
In order to exclude transport damage and to check correct installation, carry out a test run whenstarting up the centrifuge.
Procedure:
•
•
•
•
•
•
•
•
•
•
•
•
•

Connect centrifuge with the power cord to the power supply.
Press “OPEN/(EMERGENCY STOP)“ 0 button.
“UNLOCKED“ display lights up.
Turn lid knob in counterclockwise direction and open lid.
There should be no foreign bodies in the rotor chamber.
Turn rotor by hand and check whether it runs freely and horizontally and that the screw in the
middle of the rotor is tightened firmly. If this is not the case, the centrifuge must not be put into
operation.
Insert empty tube holder into the rotor and check that it is fitted correctly.
The rotor must be loaded evenly.
Close lid and turn lid knob in clockwise direction to end position.
“LATCH“ display lights up.
The centrifuge will only start running if the lid has been closed correctly.
Press “START“ button and perform a test run.
“RUN“ display lights up.

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Operating Instruction Centrifuge LC 6••

Apparatus accelerates, tube holders slip into a horizontal position.
Pay attention to the running noise of the centrifuge. There should be a soft humming. In thecase of loud or unusual noise stop centrifuge immediately by pushing the“OPEN/(EMERGENCY STOP)“ button and then check the above points once again.
Press “OPEN/(EMERGENCY STOP)“ button.
The test run is ended and the rotor braked to a standstill.
The centrifuge is now ready for operation.

•••

7. Operation
Control Elements

2
1
4
5
3

6
7

8
1
4

Lid
„RUN“

2
5

Lid knob„LATCH“7“OPEN/(EMERGENCY STOP)“ button

8“START” button

3
6

Safety lock
„UNLOCKED“

Setting the time
Checking the set centrifugation time
Connect centrifuge to the power supply using the power cord.
Deactivate the interlocking mechanism by pressing the “OPEN/(EMERGENCY STOP)“
button and open the lid.
Press down the “START“ button for approximately 3 seconds.
When the “LATCH“ display starts to flash, release the “START“ button.
The “RUN“ display flashes once for every minute set.
Changing the set centrifugation time
Deactivate the interlocking mechanism by pressing the “OPEN/(EMERGENCY STOP)“
button and open the lid.
Press the “OPEN/(EMERGENCY STOP)“ and “START“ buttons simultaneously and holddown for approx. 3 seconds.
Release the buttons once the “LATCH“ display starts to flash.
The set time is reset to 0 once the switch is released.
Set the requisite centrifugation time using the “START“ button;press the “START“ button once for every minute (max. 30 minutes).
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Operating Instruction Centrifuge LC 6
Press the “OPEN/(EMERGENCY STOP)“ button 3 times until all display lights havestopped flashing and are off, to confirm the set time.
Recommended conditions for centrifugation of S-Monovettes with gel preparation:
Ø Tube holder 100 mm
Ø Serum-gel

g-forceØg-force

Li-Hep-gel

2,000 x g (= fix set) / 15 min / 20 °C(within 4 hours after blood collection)
2,000 x g (= fix set) / 20 min / 20 °C(within 4 hours after blood collection)

Concerning centrifugation conditions for other type of blood collection tubes refer to data provided
by manufacturers.

Counter
The cycle counter keeps a count of the total number of machine runs for servicing and maintenancerecords. The counter is located at the lower front side.

Access to the Rotor Chamber during a Power Failure
In the event of a power failure, it may be impossible to unlock
the lid in the usual manner. If this happens, you can gain accessto the rotor chamber by removing the label in front of the lid lockand manually deactivating the locking mechanism with a pen(see illustration). Pull the mechanism in the direction of thecontrol panel, unlock it and open the lid. Then re-stick the label
in position.

If the centrifuge is damaged, have it checked by a technician before further use!

Loading the Rotor
The centrifuge must be evenly loaded for it to function properly.
To ensure even loading, comply with the following rules when loading the horizontal rotor for
six tube holders:• Opposite-located tube holders must be identical.
• Opposite-located tube holders must either be empty or loaded with the same
number of samples of the same weight.
• When centrifuging an uneven number of samples, insert a tube filled withwater as a counterbalance.

Centrifuging
If a problem occurs during operation which makes it necessary to switch off thecentrifuge, push the “OPEN/(EMERGENCY STOP)“ button immediately.

Procedure:••

Press “OPEN/(EMERGENCY STOP)“ button to unlock the lid and open lid.
Insert specimen tubes in the tube holders. Make sure they are evenly loaded.

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Operating Instruction Centrifuge LC 6
•
•
•
•
•
•

•
•
•

Close lid and turn lid knob to end position in clockwise direction. The “LATCH“ display lights
up in order to show correct interlocking.
If the lid knob is not snapped in completely, the “LATCH“ display will not light up and it will
not be possible to put the centrifuge into operation.
Press the “START“ button.
The rotor will start to turn. The “RUN“ display lights up.
At the end of the running time the “RUN“ display goes out and the rotor break is applied
until it comes to a complete standstill.
The “UNLOCKED“ display lights up; the interlocking mechanism is deactivated thereby
facilitating access to the rotor chamber. After expiry of the automatic unlocking time the lid
can be unlocked with idling rotor using the “OPEN/(EMERGENCY STOP)“ button.
Turn lid knob in an anticlockwise direction and open lid.
Remove specimens.
The centrifuge can be put into operation immediately again.

8. Maintenance and servicing
Service address
Should you have questions or problems concerning the device, please contact the customer service
department of DESAGA GmbH / SARSTEDT-GRUPPE in Wiesloch or your local SARSTEDT sales
office. Remember to specify the Serial no. of the device.
DESAGA GmbH / SARSTEDT-GRUPPE
Serviceabteilung
In den Ziegelwiesen 1-7
D-69168 Wiesloch
Phone: +49 (0) 62 22 / 92 88 65
Fax.:+49 (0) 62 22 / 92 88 60•••

Fill in the Contamination Questionnaire (Page 18) and ship it together with thedevice, a copy of the delivery note, and a short description of the problem.
Always ship the device in the original packaging.
The Contamination Questionnaire serves to protect our service personnel.
Therefore, please fill it in carefully and completely.

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Operating Instruction Centrifuge LC 6

Cleaning
Potential danger of contamination

The regulations for cleaning the device must be observed. Inattentive cleaningor non-observance of the regulations can cause malfunctions!
•

Disconnect the device from the mains supply before cleaning.

•

Disinfect the apparatus before cleaning••

No liquid may be permitted to enter the device during cleaning.
Clean the housing surfaces with a damp cloth. A soap solution may be used.
Carefully dry the device after cleaning.
Do not use scouring agents or aggressive cleaning liquids or solutions (except
alcohol) under any circumstances.
Isopropanol may be used in case of severe soiling.
Fully/partially halogenated hydrocarbons, ketones, esters, and other chemical
not specified by the manufacturer may damage the rotor and tube holders andmay not be used.

•••

Example for a disinfectant:
The following aqueous solution can be used as a disinfectant:
25 g ethanol 96 %, 35 g 1-propanol, 0,1 g glyoxal ad 100 g aqua dest.
Apply the solution, and allow it to react for a few minutes, depending on thecontamination. If necessary, repeat the application of disinfectant.
Tubeholder:
Small glass particles from a tube breakage remaining in the tube holder can stick to
the next sample tube inserted in this holder. When handling this tube, the broken glasscan penetrate protective gloves and cause injury from cuts to the user’s fingers or
hand. Broken glass remaining can result in stress points with subsequent tubes
causing further breakage. If a tube breaks, remove it carefully from the tube holder.
Dispose of the sample and tube fragments correctly, and clean both the inside andoutside of the tube holder. If necessary use a new rubber adapter and reinsert the
tube holder in the rotor.

Regular Maintenance
It is recommended, depending on the amount of use and the external conditions, thatthe rated speed and the electrical safety be checked every 1-2 years to ensure
continuing safe operation.
It is recommended that the rotor chamber be cleaned every 6 months or if a tube
breaks.
It is to replace the tube holders after 5,000 machine runs or the latest after 24 months
of use.

Index
centrifugation time 10
lid safety switch 6
Loading the Rotor 11

Machine data 8
safety notes 4
test run 9

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Operating Instruction Centrifuge LC 6

Contamination questionnaire
for repair orders
Dear Customer,
Please complete the contamination questionnaire before you return the instrument to the service
department of DESAGA GmbH / SARSTEDT-GRUPPE or to your local SARSTEDT sales
representative for maintenance or repair.
Firm:

Department:

Town:

Street:Name:
Name of
Instrument/Artikel:

Telephone No.:SN:

The instrument is free from dangerous substances
The instrument came into contact with the following dangerous substancesGroups of substances

Substance nametoxic
acridly
explosive
radio-active
contagiously danger substances
igniting subst.
Other hazardous subst.
The instrument has been decontaminated according to the legal regulations.
(Please cross off if applicable!)
Description of the decontamination:

Date:

V. 2.1.2 / 01.2009

Signature: