Operating instructions®

Sediplus S 200
SARSTEDT Item No. 90.189.900

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SEDIPLUS S 200

Operating instructions

Document information
Version:Created on:
Valid from machine version:
File:

V 3.2
16042009
S 200 V 01.03
BA-S200-E 16042009.DOC

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SEDIPLUS S 200

Operating instructions

Table of contents
Page

1. Machine data ...................................................................................................................... 4
2. Manufacturer’s address ...................................................................................................... 4
3. Important notes................................................................................................................... 4
Explanation of symbols.................................................................................................................5
Device-specific safety notes .........................................................................................................5
Obligation of the operator .............................................................................................................6
Obligation of the personnel...........................................................................................................6

4. Additional notes .................................................................................................................. 6
Warranty and liability ....................................................................................................................6
Decommissioning / Disposal.........................................................................................................7

5. Description of the device .................................................................................................... 7
6. Transport, assembly and connection.................................................................................. 7
Scope of delivery..........................................................................................................................7
Unpacking ....................................................................................................................................8
Technical data..............................................................................................................................8
Set-up ..........................................................................................................................................9
Commissioning.............................................................................................................................9

7. Operation.......................................................................................................................... 10
Operating elements .................................................................................................................... 10
Preparation of the samples......................................................................................................... 11
Inserting the SARSTEDT S-Sedivettes....................................................................................... 12
Conducting the measurement..................................................................................................... 12
Data storage............................................................................................................................... 15
Measurement values .................................................................................................................. 18
Examples of technical literature.................................................................................................. 19

8. LIS / Printer connection ................................................................................................... 20
9. Device configuration ......................................................................................................... 23
10. Maintenance and repair.................................................................................................. 25
Cleaning ..................................................................................................................................... 25
Maintenance intervals................................................................................................................. 25
Simple functional checks by the user.......................................................................................... 26
Troubleshooting.......................................................................................................................... 27
Service address.......................................................................................................................... 28

11. Glossary ......................................................................................................................... 29
Contamination questionnaire................................................................................................ 30

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Operating instructions

1. Machine data
(to be completed by the customer)
Type:________________________________________________________________________________________________________

Serial no.:____________________________________________________

Location:____________________________________________________

Date:____________________________________________________

Inventory no.:____________________________________________________

2. Manufacturer’s address
SARSTEDT AG & Co.
PO Box 1220
D-51582 Nümbrecht
Telephone: +49 (0) 22 93 / 305 0
Fax:+49 (0) 22 93 / 305 282
info@sarstedt.com
email:

3. Important notes
Prior to commissioning the device, observe the safety notes in these
operating instructions!
A basic prerequisite for the proper handling and correct operation of the device isfamiliarity with the contents of these operating instructions.
Please keep these operating instructions as fundamental information foryour device.
Copyright:
SARSTEDT-AG & Co. has the copyright of the present operating instructions. The operating
instructions are only intended for the operating personnel and the purchaser of the device.
These operating instructions may not be duplicated nor distributed in part or in full without the writtenconsent of SARSTEDT AG & Co.
Non-compliance may be punishable by law.
SARSTEDT S-Sedivette and Sediplus S 200 are registered trademarks of
SARSTEDT AG & Co.
Technical changes reserved!
Nümbrecht, May 2008
SARSTEDT AG & Co.

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Operating instructions
Explanation of symbols

These operating instructions contain the following important symbols in order to indicate dangers andoperating errors.
The “Attention” sign means that in this section:•
Important notes are given.
•
An immediate or potentially health or life-threatening danger is described.
•
A note regarding dangerous situations can be found.
Disregard of this symbol can result in minor to severe or even life-threatening
injuries, or property damage.
The high voltage symbol means:
Warning of dangerous, electric current!

This "Info symbol" gives advice for the proper handling of the device or gives general
information for better understanding. Disregard of the notes can lead to defective
operation or even damage to the device.
Additional symbols contain notes for the theme represented in the symbol, like
accident prevention guidelines, power supply, maintenance, or disposal.
The warning symbol means:
Warning of irritants or materials that are harmful to your health!
•
Disregard of this symbol can lead to severe damage to your health.
•
Constantly observe applicable accident prevention guidelines when handlingthese materials.

Device-specific safety notes
This device is built state-of the-art and according to the recognised technical safety
regulations.
The applicable safety notes and guidelines, as well as work safety and accident
prevention guidelines for application in a laboratory must be observed.
Nevertheless, dangers for the user, for third parties, and/or disturbances on the device
or other material assets may arise during operation.
The device may only be used:• for the designated use.
• in technically-sound, properly-functioning condition.
The device may not be operated in potentially explosive areas.

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SEDIPLUS S 200

Operating instructions
The device conforms to international safety regulations.

The device is operated through an external power supply with a voltage of 12V DC.
The following notes must be adhered to without fail:• No water or other fluids may penetrate into the equipment.
• Only the supplied wall plug transformer may be used for connecting the device
to the network.
• Check the connecting cable for damage before commissioning. Damaged
cables should not be used in any case.
The device should only be operated or completed with accessories that are expressly
intended for it.
The manufacturer recommends inspection of the device at intervals of two years,
in accordance with the applicable accident prevention guidelines (repeated inspectionof electrical equipment).

Obligation of the operator
The operator of the device is obligated to only assign work on the device to personnel that have readand understand these operating instructions. This should be confirmed by their signature.
Personnel should be checked periodically to ensure regular working within safety guidelines.

Obligation of the personnel
Persons working with the device for the first time are obligated, prior to commissioning:• To read through these operating instructions.
• To observe the applicable regulations and the guidelines for accident prevention.

4. Additional notes
Warranty and liability
In principal, the “Terms of delivery and payment” of SARSTEDT AG & Co. apply. These are noted on
the back-side of the invoice.
During the warranty period, the device may only be repaired by SARSTEDT AG & Co. or by persons
authorised by SARSTEDT AG & Co.
With improper handling or repair, any warranty claims expire.
Warranty and liability claims are excluded if they result from one or more of the following causes:•
Improper use of the device.
•
Improper assembly, commissioning, operation, or maintenance of the device.
•
Operation of the device with defective safety equipment, improperly attached, or nonfunctional safety and protective devices.
•
Non-adherence to the notes from the operating instructions in regard to transport,
storage, assembly, commissioning, operating, maintenance, set-up, and disposal.
•
Arbitrary changes to the device.
•
Disaster situations through impact of foreign objects and higher powers.
•
Improperly performed repairs.

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Operating instructions
Decommissioning / disposal••

The device must be handled and disposed of competently and properly inaccordance with the applicable regulations.
The substances that are used and/or introduced in combination with this device
must be handled and disposed of competently and in accordance with theapplicable regulations.

These operating instructions consist of the following materials:
The cover consists of PVC, the rest of paper.

5. Description of the device
The 10-channel blood sedimentation measuring device Sediplus S 200 is based on the extensive
experience of SARSTEDT in the area of blood sedimentation. Through the use of the most modern
micro-controller technology, automatic, easy-to-operate blood sedimentation is available in the
Sediplus S 200.
The sedimentation values measured in the SARSTEDT S-Sedivette are converted by the device
into Westergren values. The user continues to work with the customary data.
Using the Sediplus S 200, either 10 sedimentations can be measured simultaneously or
independently from one another. Ongoing checks by the built-in micro-controller ensure the optimalfunctioning of the measurement system.
During power failure the automatic data storages saves the measurement values that have alreadybeen calculated.
No battery is necessary for data protection.

6. Transport, assembly and connection
Scope of delivery
Standard scope of delivery
90.189.900

Sediplus S 200 incl.
Mains adapter 12V DC, 1.25A, negative pole earthed
Mains cable

Accessories / spare parts:
92.189.910
92.189.911
92.189.915
92.189.930
92.189.935
90.189.720
90.189.750
90.180.700
90.180.600
92.180.615

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Mains adapter 12V DC, 1.25A, negative pole earthed
Mains cable
SediTest S 200
Barcode reader with wedge interface for S 200
IBM keyboard with PS/2 plug
Thermal printer with mains adapter 230/6
Sediplus® Data-Manager
Sarmix® M 200 (for 4 S-Sedivettes)
Sarmix® M2000 (Mixer for S-Sedivette®)
Block rotor for M 2000 (for 40 S-Sedivettes)

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SEDIPLUS S 200

Operating instructions

Should you have questions regarding additional SARSTEDT BSS machines or the SARSTEDT
product range, please contact:
SARSTEDT AG & Co.
PO Box 1220
D-51582 Nümbrecht
Telephone: +49 (0) 22 93 / 305 0
Fax:+49 (0) 22 93 / 305 282
or your local SARSTEDT seller in connection.

Unpacking••••

Check the integrity of the packaging carton and the device.
Immediately inform the carrier of transport damage! Observe the notification periodof the individual transport companies (rail, post, parcel service or carrier). Owing tocircumstances it is only 24 hours.
Check scope of delivery.
Defects and damages must be reported immediately to SARSTEDT AG & Co.!

Technical data
Device data
Device:Manufacturer
Order number:
Measuring principle:
Measuring method:
Measurement range:
Measurement precision:
Display:Interfaces:
SupplyElectrical connection:

Sediplus S 200
SARSTEDT AG & Co.
90.189.900
Reflection measurement IR 880 nm
Measurement in the blood sedimentation device Sediplus S
200 in combination with the SARSTEDT S-Sedivettes
0-63 mm absolute (=0-109 mm Westergren)± 1 mm of the measured displacement
16-digit LC display with 9 mm character height
Lapsed measurement time in the form h:mm:ss
Measurement value in mm (according to Westergren for 1h and2h)
10 3-colour LEDs for status indication of the measurement
channels.
RS232 for EDV connection or serial printerPS/2 connection for IBM keyboard and /or barcode reader.

Active output:
Fuse:

Mains plug with connector for non-heating apparatus:
Input voltage: 100V~ – 240V~ 50/60 Hz
12V DC 1,25A; negative pole earthed.
short circuit protected
15 W maximum
None

Ambient conditions
Allowable ambient
temperature:
Maximum relative humidity:

Working temperature (18°C to 23°C), or according country- orlaboratory specific standards
80 % not condensing

Dimensions
Width x depth x height:
Weight:

300 mm x 300 mm x 160 mm
2.6 kg without mains adapter

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SEDIPLUS S 200

Operating instructions
Set-upBecause the BSS can be influenced by numerous sources of error, the following should be observed
when setting up the machine:
•

•

•
•

Place the machine on a solid, vibration-free and even surface.
The machine must be set up vertically because an inclined position can lead toincorrect sedimentation results.
Deviations from working temperature (see technical data) can lead to changes ofthe sedimentation results.
Therefore the machine should not be set up in the vicinity of radiators or othersources of heat.
Direct solar radiation or light radiation from the front or from the side must be
avoided.
Always keep the measurement lens clean. In particular, blood or plasma spattershould not get into the lens.

Commissioning
Attention with the initial start-up!
• Through the use of the mains adapter (100V~ – 240V~) the machine has a wide
range of operation independent of the available mains voltage. Nevertheless,ensure that your mains supply lies within the indicated range.
• Observe permissible ambient conditions for the device. (see Page 8 Ambientconditions)• During the cold season, after delivery and unpacking, wait approx. 30 to 45
minutes until the device has assimilated to working temperature (see technicaldata).
Insert the round plug of the mains adapter into the device socket on the back side of the
Sediplus S 200.
The On / Off switch is likewise located on the back side of the device.
After switching on, the device carries out internal checks and initialisations. During this time all LEDs
are energised in the 3 different colours: green, red, and orange. The following text appears in thedisplay:S A R S T E D T
After completion of the initialisation the following text is displayed:

S 2 0 0V x x .

y yxx.yy = current firmware version number e.g.: 01.00
At this point in time 1 or more of the LEDs may illuminate.
The following colours are possible:
•

•

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Green LED:

An applicable measurement value is present in thecorresponding channel,
which can be displayed by pressing the channel button.
Red blinking LED: An ongoing measurement was interrupted in this channel by apower failure (see error messages).

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SEDIPLUS S 200

Operating instructions

7. Operation
Operating elements
The operation of the Sediplus S 200 takes place interactively The following input and/or output
elements are available to the user for this:
Input

Buttons 1 – 10
START button
ESC button
Optional barcode reader and/or keyboard
LC-Display 16-digit
3-colour LED per channel
Acoustic signaller

Output

1

2
3

6
4

Index
1
2
3
4
5

5

7

9

8

10

Description
Loading position (channel)
Channel status LED[START] button[ESC] button
16-digit LC display

Index
6
7
8
9
10

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Description
Channel buttons [1] .. [10]On/off switch
Socket for mains plug
EDV connection SUBD socket 9pole.
Connection for keyboard and/or
barcode reader
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SEDIPLUS S 200

Operating instructions

Preparation of the samples
•
•
•

•

•

•
•

Only use the SARSTEDT S-Sedivette.
Observe the notes for handling on the packaging of the SARSTEDT S-Sedivette.
The surface of the S-Sedivette must be clean. No residue from labels or traces ofpowder from gloves may be on them. Additional signals that arise from impurities
are registered and can considerably influence the evaluation of the sedimentation.
The original label or a label affixed at the same height will not disrupt themeasurement.
While inserting the S-Sedivette no droplets of blood should be in the cap, and thecone of the S-Sedivette should not be wet. The measurement could be falsified
by blood dripping down.
The samples should not be moved directly from the refrigerator and placed in the
S 200 for analysis. For the accuracy of the results it is crucial that the samples arealways at working temperature (see technical data).
No blood or plasma spatter should get onto the measurement lens.
If your own label is applied, it must be ensured that it is not affixed significantly
higher than the original label of the S-Sedivette. Your own label may be applied amaximum of 6 mm above the original label.
A label that is applied too high will lead to an error message and the interruption of
the measurement.

The blood sample is conducted with the SARSTEDT S-Sedivette with sodium citrate solution as an
anticoagulant. The S-Sedivette is a closed blood sampling system that simultaneously serves as asedimentation tube. You do not have to transfer blood or fill a separate pipette.

•
•

V 3.2

Every blood sample must be thoroughly mixed immediately after extraction
and immediately before inserting into the device, but without creating foam.
Use the obtained blood sample as soon as possible. If the sample is stored atroom temperature, the analysis should be conducted within 4 hours. The blood
sample should not have coagulated.

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SEDIPLUS S 200

Operating instructions
Inserting the SARSTEDT S-Sedivettes
•

•
•
•

The S-Sedivettes must be correctly filled for the blood sedimentation. For thisit must already be ensured during the blood extraction that the SARSTEDT SSedivettes are handled according to the handling notes on the packaging.
The S-Sedivettes are filled correctly if the plunger is pulled out to the "snapposition".
With an underfilling it is up to the operator’s discretion whether the BSS values
must be doubted.
The blood volume in the Sedivette column influences the sedimentation value.

Insert the SARSTEDT S-Sedivette as shown in the drawings below. For organisational reasons,however, it is generally advisable to place the first sample in the first measuring point, the second
sample in the second measuring point, etc; this means that the 11th sample should be placed in thefirst measuring point. In doing so it is irrelevant in what time intervals the measurements are started,because the time counting for each measuring point takes place separately and the samples aremeasured completely independently of one another.

In order to avoid wrong signals on the measurement lens from the labels attached to
the S-Sedivette, you should make sure that any gap between the ends of the label
face toward the user after inserting the S-Sedivette.

Conducting the measurement
ID Number
After the sample is placed in the measuring point the measurement can begin. For identification of
the measurement an ID number is assigned to it. The ID number involves a 12-digit character string
witch can be entered in 2 different ways.
1. Auto–ID
The device assigns an automatic ID number that is increased by one for eachmeasurement. The last-used Auto-ID remains saved even after switching off thedevice. After switching back on, the next start of a measurement is continued withthe last used Auto-ID + 1.
For resetting the Auto-ID, see “Starting the measurement”.

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SEDIPLUS S 200

Operating instructions

2. Input through barcode reader, or keyboard
The ID number can be entered by reading off through an optional barcode reader
or an IBM keyboard with PS/2 plug.
A max. 12-digit alphanumeric barcode is accepted.
The barcode reader must be prepared so that it ends with the characters read off
with CRLF. See the user's guide of the barcode reader.
The following characters can be entered by the keyboard:•

All characters between ASCII code 32 and 127 (decimal), the button [◄—] forentry corrections, and the button [ENTER].

For differentiating between an Auto-ID and a numeric barcode, the Auto-ID number isalways filled out with the most prominent 0 to 12 positions, whereas with entry throughthe keyboard or barcode reader, the most prominent blank characters are filled out in
12 positions.
Starting the measurement•

Place the sample in the desired remaining available position (channels).
If all channels are available, their LEDs either do not illuminate, illuminate in thecolour green, or blink red after power failure.

•

Press the button [START].
The device issues an Auto-ID to the measurement and prompts the user to start
the measurement in the desired channel by pressing a channel button (1-10). A
continuous tone is released by the internal signaller and the following display
appears (in the example Auto-ID = 17)

0 0 0 0 0 0 0 0 0 0 1 7

C .

?

You now have 10 seconds to react to the prompt described as follows:-

Interrupt by pressing the [START] button again.
An interruption also cancels a previous resetting of the Auto-ID!
Resetting the Auto-ID to the value 000000000001 by pressing the [ESC]
button.
Overwriting the Auto-ID by reading off a barcode, or entry through akeyboard.
Conclusively starting the measurement by pressing the channel button that is
assigned to the position in which the sample was placed.

If the start of the measurement does not take place within 10 seconds, the starting
process is interrupted.
ATTENTION:
Resetting the Auto-ID to the value 000000000001 does not delete any measurement
results already saved.
Be careful when resetting the Auto-ID in order to avoid double allocations of ID
numbers. An inadvertent reset can be undone with [START]!

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SEDIPLUS S 200

Operating instructions

If you are not working with your own ID numbers (barcodes) then there is no reason toreset the Auto-ID, unless you would like to begin the ID numbers on each work daywith 000000000001.
Writing over the Auto-ID with a barcode reader or keyboard does not change the valueof the previous Auto-ID.
The Auto-ID reaches the maximum value of 000000999999. 000000000001 is given
again as the next number.
Process of the measurement
After successfully starting the measurement the samples to be measured are scanned in thefollowing listed intervals:
Time point
Function
immediately
Check of the following criteria:- Sample present ?
- Sample over-filled ?
- Sample under-filled ?
- Label affixed too high ?
The measuring time for the current channel begins to run
The LED of the current channel illuminates red.
During the on/off movement of the measuring head this display appears:

C h a n n e lafter 30 sec

a r

t

1

O f

f

s e t

Evaluation of the 1h value
The LED of the current channel illuminates orange
Display:C h a n n e lafter 120 min

S tFill level of the sample.
The value determined here applies as the starting point (zero point) for the
additional measurements of the 1h value, and/or the 2h value.
Within these 30 sec. blood droplets can manage to run out of the screw cap andyield a different fill level than was the case during the 1st measurement.
The LED of the current channel illuminates red:C h a n n e lafter 60 min

1

1

1 h

Evaluation of the 2h value
The LED of the current channel illuminates green
During the on/off movement of the measuring head this display appears:

C h a n n e lPage E-14

1

2 h

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SEDIPLUS S 200

Operating instructions

During the measurement the process status for each channel can be called up individually.
To do so, press the desired channel button.
One of the following listed readouts appears in the display.
During the 1st hour of the measurement
Channel number (here 1) and the lapsed measuring time (here 0:17:32 hours)

0 1

0 :

1 7 :

3 2

During the 2nd hour of the measurement
Channel number (here 1), sedimentation value after 1 h (here 2 mm) and lapsed
measuring time (here 1:22:10 hours)

0 1

2 m m

1 :

2 2 :

1 0

After the end of the measuring time
Channel number (here 1), sedimentation value after 1h (here 2 mm) and after 2 h (here
5 mm)

0 1

2 m m

5 m m

Interruption of a measurement in progress
A measurement in progress can be interrupted by the user at any time. In order to interrupt a
measurement, proceed as follows:
Press the desired channel button (in the example Channel 6).
One of the status displays shown above appears.
Press the [ESC] button
The following display appears, which prompts you to confirm the procedure.

0 6

I

n t

e r

r

u p t

?

The following reactions are possible:[ESC] button:
The measurement is not interrupted[START] button
The measurement of the selected channel is interrupted

Data storage
The SARSTEDT Sediplus S 200 is equipped with a NV-RAM memory whose content is not lost afterswitching off the power supply.
The following data is saved in this memory.

V 3.2

•

All configuration data•

The data of the measurements in progress of all channels•

The measurement results of a max. 125 samples
The measurement results remain saved until they are called up by an EDV systemor a serial printer. Measurement results that are called up are deleted from thememory.
If no measurement results are called up, then the oldest results are overwritten
according to the FIFO principle, as soon as the data storage is full.
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SEDIPLUS S 200

Operating instructions
Display of the last measurements of the individual measuring channels

Only the last measurement results of a measuring channel can be shown on the display of the
Sediplus S 200. A green LED signals that a result is available in a measuring channel.
This result can also be an error message!
It is available until a new measurement is started in this channel or the last result of a channel ismanually deleted.
By pressing the desired channel button the following information is displayed:•

ID number of the measurement
Press the channel button. As long as the button is pressed and held, the channel
number (left-aligned) and ID number (right-aligned) of the measurement are
displayed.
Here Channel 1 ID Number Sample ID (Entry through keyboard)

0 1•

S a m p l

e -

I

D

Current status of the measurement, or last result of the measurement
After releasing the channel button the display changes as follows:
Depending on whether a measurement is already running in the selected channel
or results are already available, either the current process status or the result of
the last measurement is displayed.

0 1

0 :

1 7 :

3 2

0 1

2 m m

0 1

2 m m

5 m m

0 2

5 7 m m

> 1 1 0 m m

0 3

7 2 m m

N P !

0 4

N P !

7 5 m m

0 5

N P !

N P !

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1 :

2 2 :

1 0

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SEDIPLUS S 200

Operating instructions

Special cases:
The following exceptions have a special meaning:•>110mmThis value is given if a clear sedimentation value of more than 110 mm wasrecognised. This sedimentation applies as “accomplished”.

•

NP! (not plausible)
This value is given if no clear sedimentation value was recognised or therecognised transfer appears implausible.
Therefore it could be that the 1h value is identified as implausible or unclear, but
the 2h value was clearly recognised.

Manual deletion of the last results of an individual measuring channel•

Select the desired channel by pressing a channel button(here Channel 6)•

Press the button [ESC]. You will by prompted to confirm the process through thefollowing display

0 6

D e l

e t

e

?

The following reactions are possible:[ESC] button:
The last result from Channel 6 is notdeleted.
[START] button
The last result from Channel 6 is deleted.
Manual deletion of the last results of all channels•

Simultaneously press the buttons [START] and [ESC]
You will be prompted to confirm the process through the following display:

D e l

e t

e

c h a n n e ls ?

The following reactions are possible:[ESC] button:
Nothing is deleted.
[START] button
The results of all channels aredeleted.
IMPORTANT:
Even if the last measurement result of a channel no longer appears in the display
because of a new measurement or manual deletion, it is still held in the data storage
until it is called up by the EDV or sent out to a printer.
There is no compelling reason to delete the results of individual channels or all
channels.
For some users, however, it may be easier if they can delete the results from achannel that they no longer need, because in doing so the corresponding LED
automatically goes out. The user then immediately recognises whether they havealready read off sedimentation values (LED dark) or not (green).
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SEDIPLUS S 200

Operating instructions

Here, each user must individually find a method of operation that is suitable for them.
Manual deletion of the results in the data storage
There is no function available to the user to delete the results of the data storage. Thisis reserved for the service functions.

Measurement values
The Sediplus S 200 calculates the sedimentation speed of the erythrocytes and converts thesedimentation range to x mm/1h according to Westergren and/or y mm/2h. All results are shown inthe display and can be exported through the RS 232 interface. No additional conversion of thesedimentation values is necessary. The values after 1h correspond to the values according toWestergren.
An evaluation after 2h is also possible in the Sediplus S 200. In this case, as with the sedimentation
system in Westergren tubes, it must be taken into consideration that the increase of sedimentation in
the second hour is slower with higher sedimentation values than in the first hour.
•
•
•
•

•

Always note identification numbers together with the patient details in order tomaintain a clear arrangement of the diagnosis data.
Log room temperature with the results in order to be able to clearly evaluate
the sedimentation values attained.
Observe that deviations from the recommended working temperature (seetechnical data) can significantly change the sedimentation values.
Also observe disturbance factors described in the technical literature e.g.
paraproteinaemia, lipaemia, haematolysis, significantly increased leukocyte
level, e.g. during CLL (chronic lymphocytic leukaemia), that can have an
influence on the measurement of the blood sedimentation and, if necessary,check the plausibility of the measurement results with unusual blood samples.
Check the functionality of the sedimentation device at regular intervals, inwhich you compare the sedimentations evaluated with the sedimentation
values read from the scale. Should you determine large deviations, werecommend having the device inspected.

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Operating instructions

Examples of technical literature
1) German Norm, haematology, DIN 58935-1
Determination of the erythrocyte-sedimentation speed in blood
Part 1: Selected method, December 1997.
2) British Standard Specification for Westergren tubes and support for the measurement oferythrocyte sedimentation rate, BS 2554:1987.
3) L. Thomas: Labs and Diagnosis. 5th Edition.
Die Medizinische Verlagsgesellschaft Marburg 1998.
4) Methods for the Erythrocyte Sedimentation Rate (ESR) Test – 4th Edition; Approved Standard.
NCCLS Document H2-A4, 2000.

V 3.2

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SEDIPLUS S 200

Operating instructions

8. LIS / Printer connection
The SARSTEDT Sediplus S 200 is equipped with a serial interface RS232.
A lab LIS, the Sediplus® Data-Manager, or a printer with serial interface can be electively connectedto this interface.
The connection between Sediplus S 200 and an IBM compatible PC can be made with a normal,
standard 1:1 interface extension cable. The Sediplus S 200 provides a 9-pole SUBD socket on theback side of the device.
The necessary connection for the data flow control of the control lines RTS/CTS is already provided
internally in the SARSTEDT Sediplus S 200.
For connection with other instruments a corresponding cable must be used.
Refer to technical data of the instrument to be connected.
The SUBD socket of Sediplus S 200 is wired as follows:
•
2
TXD
•
3
RXD
•
6
DSR
•
7
RTS
•
8
CTS
For transferring the data no action on the part of the user is required. The connected device forwards
the available data in the Sediplus S 200 by activating its RTS line and stops the transfer bydeactivating this line, if the option "Flow contr." is set to RTS/CTS. If the option is set to NONE, theresult data is transferred immediately after it is available.
The measurement results in the data storage are only deleted when they are completely transferredto the LIS, the Data-Manager, or the printer. If the device is switched off, this data set is completelytransferred again after switching the device on, as long a data set was not completely transferred.
The LIS interface is pre-set with the following transfer parameters, which can be partially changed
with the aid of the device configuration.
Parameter
Baud rate
Data bits
ParityStop bits
Data format
Data flow control

Value
9600
8
None
1
formatted
RTS/CTS

Alternatives
2400, 4800, 9600, 19200
none
none
2
compact
NONE

Analogously to older Sediplus S 200 devices, the data is transferred through the RS232 interface informatted form. That has the advantage that the data is represented in an easily-read tabular form in
the readout on a printer.
Alternatively, the data can also be transferred in compact form, bounded by STX and ETX.
You can choose between the two data formats in the device configuration.
The data blocks of the two data formats are described in the following:
Data formatted:
The data is transferred line-by-line in ASCII format. A total of 7 lines are sent per data set, eachcompleted with CRLF.
The lines are composed as follows:Line Content
C L
1
r f

RemarkBlank line
Page E-20

Length [byte]2
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SEDIPLUS S 200

Operating instructions
2
3

C L
r f

4
5

C L
r f

6

••••••60•min••aaaa•mmCrLf

7••••••120•min••bbbb•mmCrLf••••••ID-No.xxxxxxxxxxxxCrLf••••••Pos.•yyCrLf

Blank line
6 blank spaces at the beginning of
the line
Blank line
6 blank spaces at the beginning of
the line
6 blank spaces at the beginning of
the line
6 blank spaces at the beginning of
the line

2
26
2
15
24
24

Total length of the data set = constant = 95 characters
Data compact:
The data is transferred as a string in ASCII format. The string begins with the control character STX
and ends with the control character ETX. No additional control characters apart from STX and ETX
are used. The string is composed as follows:SE

txxxxxxxxxxxxxyyaaaabbbb tx

Place holder

Meaning

S

Start of text (Hex 02) Beginning of the data block
Alphanumeric ID number, right-aligned
Numeric channel number, right-aligned
Sedimentation value after 1h, alphanumeric
Sedimentation value after 2h, alphanumericEnd of text (Hex 03) End of the data blocktx

xxxxxxxxxxxx
yy
aaaa
bbbb
E

tx

Number ofcharacters
1
12
2
4
4
1

Offset in
the String
0
1
13
15
19
23

Key (applies for both formats):
Place holder
S

tx
E
tx
C L
r f

xxxxxxxxxxxx
yy
aaaa

bbbb

•

V 3.2

MeaningStart of text (Hex 02)
End of text (Hex03)
New line (2 characters Hex 0D0A)
ID number (12-digit alphanumeric), right-aligned
Channel number (2 characters, numeric without leading 0)
Sedimentation value after 1h (4 characters, numeric without leading 0)
Special cases: ">110" = Sedimentation is accomplished" NP!" = Result is implausible
Sedimentation value after 2h (4 characters, numeric without leading 0)
Special cases: ">110" = Sedimentation is accomplished" NP!" = Result is implausible"" = No 2h result available (BSS mode fast).
Blank space (Hex 20)

Page E-21

SEDIPLUS S 200

Operating instructions

ATTENTION:
In line 3 of the formatted data set there is an incompatibility to earlier devices that only
support a 4-digit numeric ID number. In order to practicably configure the entry
through the keyboard and/or barcode reader, this field was expanded to 12
alphanumeric characters.
In lines 6 and 7 the sedimentation results are displayed in 4-digits in order to be able
to carry out a better differentiation between implausible results (NP!) andaccomplished sedimentations (>110). With the older machines the sedimentation
result was always displayed numerically in 3 digits. Implausible or accomplishedsedimentations were designated by the value 120.
The complete data format did not exist with earlier devices.

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SEDIPLUS S 200

Operating instructions

9. Device configuration
In order to adapt the SARSTEDT Sediplus S 200 to the requirements of the user, there is thepossibility of putting configuration data in the NV RAM.
The following settings can be used (bold = standard setting):
Parameter
Language
BSS mode
Baud rate

Stop bits
Data format

Options
DeutschEnglish1h
1h / 2h
2400
4800
9600
19200
1
2
formatted
compact

RTS
NONE
5mm – 15mm
max.
underfillingin 1mmincrements
Frequency of the 1000 Hz – 2000
signaller
Hz in 125
increments
Flow control

V 3.2

RemarkLanguage of the text output in the display.
Measurement is finished after 1h
Measurement is finished after 2hTransfer speed of the LIS connection to baud.

Number of stop bits of the LIS connection.
Formatted output of the data for the printed readout.
Compact output for pure LIS connection.
See LIS / Printer connection.
Data flow control.
See LIS / Printer connection.
Maximum tolerated underfilling of the S-Sedivette.
The volume perception of the acoustic signal depends
heavily on the frequency provided and the individual hearingof the user. The frequency of the signaller can be adjusted
here to the subjective perception of the user.
To change: Button [1] = lower frequency
Button [2] = higher frequency

Page E-23

SEDIPLUS S 200

Operating instructions

In order to get into the set-up mode for the device parameters, press the buttons [1] and [2] whileswitching on, and hold them down until the following display appears:

P a r

a m e te r

After 1 sec the following automatically appears

S p r

a c h ed e u ts c hWith the button [9] you can now select between the individual parameters.
You can change the currently displayed parameter with the button [10]. All changes are effective
immediately. If the language parameter is changed, all of the following parameters are displayed in
the new language. e.g.:

L a n g u a g e

E n g lis h

To complete the parameter entry the following functions are available.
•[ESC] button
The entry is finished. All changes undertaken remain in place as long as thedevice is switched on. After switching off the device, all changes are lost. The
device is subsequently ready for normal operation.

S 2 0 0•V x x .

y y[START] button
The entry is finished. The current parameters are saved to the NV RAM and are
kept there after switching off and back on again.
This display appears for approx. 1 sec:

V a l

u e s

s a v e d !

Afterwards the device is ready again for normal operation.

S 2 0 0V x x .

Page E-24

y y

V 3.2

SEDIPLUS S 200

Operating instructions

10. Maintenance and repair
Cleaning
Observe the guidelines for cleaning the device without fail. Improper cleaningor non-adherence to the guidelines can lead to malfunctions!
• Separate the device from the power supply for cleaning.
• No fluids may penetrate into the device during cleaning.
• Clean the housing parts with a damp cloth. A soapy solution can be used.
Carefully dry the device afterwards.
• Never use abrasives, harsh cleaners, or solvents (alcohol excluded).
• Isopropyl can be used with heavy staining.
Example of a disinfectant:
For disinfection use a water-based solution:
25 g ethanol 96 %, 35 g 1-propanol, 0,1 g glyoxal ad 100 g distilled water.
Apply solution and allow it to work for a few minutes depending on contamination. If
necessary repeat wetting with the disinfection solution.

Maintenance intervals
It is recommended to have the SARSTEDT Sediplus S 200 serviced by the
manufacturer on an annual basis.
To do so, contact your responsible SARSTEDT Service Organisation or your local
SARSTEDT dealer.

V 3.2

Page E-25

SEDIPLUS S 200

Operating instructions

Simple functional checks by the user
With the aid of a so-called “Position test” the user can test the function of their device
to a limited extent on their own. The test tool SediTest S 200 (SARSTEDT item
number 92.189.915) is required for this.
Execution:
Switch off the device and hold down the button [10] while switching back on until thefollowing display appears:

P o s i

t

i

o n

t

e s t

Now place the SediTest S 200 in one of the channels 1 thru 10 and press thecorresponding channel button. The device scans the test tool in the corresponding
channel. The following message appears:

C h a n n e lx x

T e s txx= Channel number.
Afterwards the result is shown in the display.

T r

a n s i

t

i

o n :

0 m m

The target value is 0 mm. A deviation of +/- 1 mm is acceptable.
You can repeat the test as often as you wish, however it should be carried at least
once per measurement channel in order to check all channels.
To do so, place the SediTest S 200 in another channel and press the corresponding
channel button.
The following functions are tested:•

The drive of the measuring head over the entire path of travel•

The analogous measurement value entry is checked purely qualitatively for“functions" / "does not function".
It is not a quantitative inspection of the measuring sensitivity.

•

The function of the channel switching.
In doing so it is important to always use 1 single SediTest S 200 in the device.
With the use of multiple SediTest S 200 an error in the channel switching may not
be recognised.
To finish the test press the [ESC] button.
After the measuring head is moved to its starting position the device is ready again
for normal operation.

S 2 0 0V X x .

Page E-26

y y

V 3.2

SEDIPLUS S 200

Operating instructions

Troubleshooting
In order to avoid operating errors or to independently recognise device errors, the SARSTEDT
Sediplus S200 carries out the following checks during its run time:
Check for operating errors:• Correct fill level of the S-Sedivette
• Correct placement of the label• Availability of the desired measuring channel• Functionality of the desired measuring channel
Check for malfunctions:• Monitoring of the motor drive for step loss
• Check for power failure
Check for data integrity:• Monitoring of all saved data in the NV RAM for correct check sums
As a result the following error messages may arise:
MessagePower failure!!
immediately afterswitching on

LED / Signaller
1 or several
LEDs blinking red.
Continuous tone

xx Power failure
xx= Channel no.

LED xx blinks red

Drive defect !

Continuous tone

Step loss of themeasuring head drive.

xx Drive error
xx= Channel no.

LED xx green

No results are present at
the selected channel
because a drive error wasrecognised whilemeasuring.

V 3.2

ReasonDuring a measurement
the device was switched
off or a power failure
occurred.
The measurements on
the affected channels arelost
The selected channel wasaffected by a power
failure.

Page E-27

Measure(s)Press [ESC] button. The device
can be operated again.

The channel can be usedagain.
The LED goes out after the
new start of a measurement or
can be turned off with the
button [ESC].
The device cannot be operated
again.
Switch off and switch on again.
If the message remains
notify Service.
Scan the results of all channels
and notify Service.

SEDIPLUS S 200

Operating instructions

Config. error!
immediately afterswitching on.

Continuous tone

Channel defect!

No change

Channeloccupied!

No change

All ch. occupied

No change

xx Underfilled
3 Whistle tones
xx= Channel no.
xx Overfilled
3 Whistle tones
xx= Channel no.
xx Label too high 3 Whistle tones

xx No sample

3 Whistle tones

An error of the data
integrity was detected.
The device can no longer
be operated.
You have attempted tostart a measurement thatis marked as defective.
You have pressed the
button of an occupied
channel for starting ameasurement.
You have pressed
the [START] button, but
all channels are occupied.
The tube is not sufficiently
filled.
The tube is overfilled.
The label is placed too
high on the tube
You have not placed asample in the selectedchannel.

Notify Service.
The user can no longer startthe device.
All other channels can continue
to be operated.
Notify Service.
Select the correct channel.

Wait until a channel becomes
free.
Request new material. The
measurement is interrupted.
Request new material. The
measurement is interrupted.
Peel off label and reapply, if
possible. The measurement isinterrupted.
The measurement isinterrupted. Insert sample andstart again.

Service address
If you should have questions or problems with the device, contact the service department of
DESAGA GmbH / SARSTEDT-GROUP in Wiesloch or your local SARSTEDT dealer. Be sure toprovide the serial number of the device.
DESAGA GmbH / SARSTEDT-GROUP
Service department
Ziegelwiesen 1-7
Tel.: +49 (0) 62 22 / 92 88 65
Fax.: +49 (0) 62 22 / 92 88 60

D-69168 Wiesloch•••

Fill out contamination questionnaire (see the end of the operating instructions) andaccompany with a copy of the delivery note and a brief explanation of the
problems that have arisen with the device.
Always send the device in the original packaging. If this is no longer available, thensubstitute packaging must be used to prevent damage to the device duringtransport. Responsibility lies with the sender.
The contamination questionnaire serves for the security of our service employees.
Please fill it out as carefully as possible in order to avoid delays to the repair work.

Page E-28

V 3.2

SEDIPLUS S 200

Operating instructions

11. Glossary
Anticoagulant

Material for inhibiting clotting
Sodium citrate solution (0.106 mol/l), 0.7 ml pre-dosed in the
S-Sedivette.

ASCII-Code

American Standard Code for Information Interchange.

Blood cell sedimentation
speed

Erythrocyte sedimentation rate (ESR) in x mm/h.

Channel button

Buttons of the Sediplus S200 that correspond to the individual
measuring points. The buttons are labelled with the numbers 1-10.

CR LF

2 characters of the ASCII code table for representation of the end of aline CR=Carriage return; LF=Line feed.
Is used in combination with a barcode reader as simulation of theconclusion of an entry (e.g.: <ENTER> button).

Data storage

Electronic module for saving the measurement results.

ESR

Erythrocyte (red blood cell) sedimentation rate

ETX

A line of the ASCII code table for designation of the end of a data block.
ETX = End of text. See also STX.

FIFO principle

First In First Out. Sequence of storage and calling up data from data storage.

ID Number

Identification number of a sample.

IBM keyboard with PS/2
plug

Entry keyboard, is used in the same manner as with common PCs.

LIS

Laboratory Information System

Measuring head

Vertically-moving part of the Sediplus S200 for the assimilation of
sensors that scan the Sedivettes placed in the device from bottom to
top in order to determine the sedimentation value.

Measuring point

One of 10 different sample retainers for the S-Sedivette. Is also often
designated in the document as measuring channel.

NV RAM

Data storage that does not lose its contents after switching off thedevice. No batteries are necessary for securing the data.

NP!

Designation of an implausible measurement value. NP! = not plausible!!
Samples whose measurement result is marked with NP! could not beanalysed properly by the device!

RTS/CTS

Lines of the RS232 interface for control of the data transfer between
devices. See also RS233 interface.

Serial interface
RS232

Serial electronic communications interface according to the Standard
RS232 or EIA 232.

STX

A line of the ASCII code table for designation of the beginning of a data
block. STX = Start of text. See also ETX.

Working temperature

18 °C to 23 °C, or depending on the national/lab-specific determination>110

Designation of a clearly “accomplished" sedimentation.

V 3.2

Page E-29

SEDIPLUS S 200

Operating instructions
Contamination questionnairewith repair orders
Dear valued customer,

We request that you answer all of the following questions for safety reasons before you send yourdevice to DESAGA GMBH / SARSTEDT-GROUP Wiesloch maintenance, repair,or return.
Company:

Department:

Location:

Street:Name:

Telephone no.:Device/article:

SN:

The device is free of hazardous materials
The device came into contact with the following hazardous materials
Material class

Material designation

Caustic materials
Easily flammable materials
Environmentally hazardous materials
Poisonous materials
Health-threatening materials
Radioactive materials
Bio-hazardous materials
Other materials
The device was decontaminated according to the legal guidelines.
(Please cross applicable)
Description of the decontamination implemented:

Date:

Signature:

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V 3.2