Operating Manual Sediplus® S 2000, V 1.2®

Sediplus S 2000, 230V
Operating Manual
SARSTEDT No. 90.189 700

For in vitro diagnostics

Operating Manual Sediplus® S 2000, V 1.2

Basic information!
Please note the information provided in this operating manual before
commencing use of the Sediplus® S 2000!
Knowledge of the contents of this operating manual is the basic precondition for correct
use and handling and trouble-free operation of the instrument.
Copyright:
The copyright for this operating manual resides with SARSTEDT AG & Co. The
operating manual is intended only for the operating staff and for the purchaser ofthe instrument.
This operating manual may be copied, distributed or otherwise communicated to third
parties in part or in whole only with the written agreement of SARSTEDT AG & Co.
No part of this manual may be reproduced in any way and/or copied using electronic
processing systems in any form whatsoever (photocopy or other methods) without thewritten approval of SARSTEDT AG & Co.
Infringements of the above provisions may have civil and/or criminal law
consequences.
Please keep the operating manual available as a source of basic information foryour instrument.

Right of technical modification reserved!
Sediplus®, Sedivette®, Sarmix® and Monovette® are registered trademarks of
SARSTEDT AG & Co.
Nümbrecht, August 2004
SARSTEDT AG & Co
Address of manufacturer/
After-Sales Service
SARSTEDT AG & Co
P.O. Box 1220
D-51582 Nümbrecht
Germany

Instrument data:(to be completed by the customer)
Typ:

Sediplus® S 2000, 230 V[ ] Expansion module for
Sediplus® S 2000

Serial No.:
Telephone+49 (0) 22 93-30 50

Telefax:+49 (0) 22 93-305 282

E-Mail:info@sarstedt.com

Installation location:
Date of installation:
Inventory No.:
EPROM No.:

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Operating Manual Sediplus® S 2000, V 1.2

Page

Table of contents

Basic information! ............................................................................................................................2
Table of contents ..............................................................................................................................3
1 General information.......................................................................................................................5
1.1 Symbols and Notes on Safety.................................................................................................5
1.2 Notes on Safety......................................................................................................................6
2 Introduction....................................................................................................................................7
2.1 Description ............................................................................................................................7
2.2 The "Blood sedimentation" variable .......................................................................................7
2.3 Technical data ........................................................................................................................8
2.4 Unpacking ..............................................................................................................................9
2.5 Scope of supply......................................................................................................................9
2.6 Setting-up and installation of the instrument ......................................................................... 10
2.6.1 Connection of expansion modules to the Sediplus® S 2000....................................... 11
2.6.2 Connection of a PC-AT keyboard and/or a barcode reader ........................................ 122.6.3 Entry of options .......................................................................................................... 15
2.6.3.2 "Measurement at ½h/1h" option......................................................................................172.6.3.3 "Language" option...........................................................................................................172.6.3.4 "Reset consecutive identification number" option...........................................................172.6.3.5 "Set time/date" option .....................................................................................................18
2.6.3.6 "Raise instrument plate for cleaning" function ................................................................18
2.6.3.7 "Automatic issue of consecutive identification number: Yes/No" function ......................18
2.6.3.8 "Underfilling limit 5 to 15 mm" function ...........................................................................19

2.6.4 Description of the serial interface .............................................................................. 20
3 The functional elements of the Sediplus® S 2000...................................................................... 22
3.1 The instrument plate............................................................................................................. 22
3.2 The LEDs on the instrument plate ........................................................................................ 23
3.3 The display ........................................................................................................................... 24
4 Operation of the Sediplus® S 2000.............................................................................................. 25
4.1 Switching on the instrument.................................................................................................. 25
4.2 Preparation of specimens ..................................................................................................... 25
4.3 Insertion of the Sedivette® and starting of a measurement ................................................... 26
4.4 Entry of the identification number .........................................................................................28
4.4.1 Entry of the identification number using a barcode reader (optional) .......................... 28
4.4.2 Entry of the identification number using a PC-AT keyboard (optional) ........................ 30
4.5 Viewing of values already determined during a measurement .............................................. 31
4.6 Acoustic assistance .............................................................................................................. 32
4.7 Erasure of all data ............................................................................................................... 33
4.8 Renewed print-out / Repeat print-out operation ................................................................... 33
5 Malfunctions and operating errors ............................................................................................. 34
5.1 Obstruction of instrument plate's upward and downward movement..................................... 34
5.2 Underfilling of the S-Sedivette® ............................................................................................. 34
5.3 Incorrect measurement........................................................................................................ 36
5.4 Premature removal of a Sedivette® ...................................................................................... 37
5.5 Power failures....................................................................................................................... 39

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Operating Manual Sediplus® S 2000, V 1.2

6 Cleaning the instrument.............................................................................................................. 40
7 Service and maintenance............................................................................................................ 41
8 Decommissioning and disposal ................................................................................................ 41
9 Ordering information................................................................................................................... 42
10 Guarantee and liability .............................................................................................................. 42
11 Glossary ..................................................................................................................................... 43
12 Index of illustrations................................................................................................................. 43
13 References ................................................................................................................................. 43
14 Index ........................................................................................................................................... 44
15 Contamination Questionnaire................................................................................................... 44
16 Annex ......................................................................................................................................... 46
16.1 Initialization of the optional barcode reader ........................................................................ 46
16.2 Serial connecting cables..................................................................................................... 48

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Operating Manual Sediplus® S 2000, V 1.2

1 General information
1.1 Symbols and Notes on Safety
This operating manual contains the following important symbols to draw attention to hazards andpotential operating errors. The "Notes on Safety" contain information on the safe handling and use of
the Sediplus® S 2000.
This "Attention" symbol signifies that the section indicated contains:• important notes• a description of a direct or possibly threatening hazard for life and limb
• a reference to potentially dangerous situations
Failure to adhere to this symbol may result in injuries ranging from slight topotentially lethal and/or to damage to property.
Warning concerning hazardous electrical voltages!
This "Information Symbol" provides an indication of information concerning thecorrect use and handling of the instrument and/or of general information forimproved comprehension. Failure to adhere to these notes may result in the
instrument being incorrectly operated or even damaged.
This "Tip" symbol provides the reader with tips concerning use of the
instrument or particularly useful information for optimum utilization of the
instrument.
Further symbols indicate the subject stated by the symbol, such as the
Accident Prevention Regulations, for example, the necessary power (voltage)
supply, maintenance and/or disposal, etc..

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1.2 Notes on Safety
The Sediplus® S 2000 blood sedimentation analyzer is designed and constructed inaccordance with the latest technological status and the acknowledged rules of safety.
Please note and adhere to the relevant notes on safety and guidelines and to theIndustrial Health and Safety and Accident Prevention Regulations for use in thelaboratory.
It is nonetheless possible during use of the instrument for hazards to occur for theuser, for third parties and/or for the instrument itself and/or other property.
The instrument is intended for use only:• for the proper and correct use• in perfect safety-relevant condition
During the guarantee period, the instrument may be repaired only by SARSTEDT AG& Co or by persons authorized by SARSTEDT AG & Co. Incorrect/unauthorizedhandling and/or repair of the instrument will result in loss of validity of any and all
guarantee claims.
The instrument is operated at a voltage of 230 V, 50 to 60 Hz. No water or otherliquids may be allowed to enter the instrument.
• Only the mains connecting lead supplied with the instrument may be used forconnection of the instrument to the mains supply.
• Always check that the mains voltage accords with the data shown on the
instruments model plate. The instrument may be connected only to an earthed(grounded) safety-contact plug socket.
• Always check the connecting lead for damage prior to use of the instrument.
Damaged leads must never be used.
• Always check the instrument itself for visible exterior damage before use!
Never use a damaged instrument.
• Always remove the plug from the socket if the instrument is not to be used for a
prolonged period and also if it is to be cleaned.
The Sedivettes and the blood specimens in the Sedivettes must under all
circumstances be correctly handled and disposed of. Please note and adhere to theregulations applicable in your country and adhere to the safety regulations in yourlaboratory.
The instrument may be used and augmented only with the accessories described inthis operating manual.
THE OPERATOR'S OBLIGATIONS

The operator of the instrument is obliged to entrust work with and on the instrument
only to persons who have read and understood this operating manual. This fact
should be confirmed by means of the signature of such persons.
The safety-aware manner of working of the operating staff should be checked atregular intervals.
THE OBLIGATIONS OF THE OPERATING STAFF
Persons who perform work with or on the instrument hereby undertake:• to read this operating manual prior to commencing work with or on the
instrument and• to note and adhere to the applicable rules and regulations for prevention of
accidents
Both of these undertakings should be confirmed by the signature of the relevant
persons.

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Operating Manual Sediplus® S 2000, V 1.2

2 Introduction
2.1 Description
Thanks to the use of an infrared-transmission measurement process and of ultra-modern
microprocessor electronic technology, and on the basis of SARSTEDT's many years of experience inthe field of blood sedimentation, the Sediplus® S 2000 constitutes an automatic forty channel blood
sedimentation analyzer.
The Sediplus® S 2000 has been specially developed for large-scale laboratories with a high numberof specimens requiring processing and central blood sedimentation processing. After completion of
measurements, the results can be fed into a computer system. Manual viewing of data is, of course,
possible at any time using the LCD display.
The basic module, with 40 measuring places, is made up of the operating panel and can be expanded
up to a maximum of 160 measuring places, with up to three expansion modules.
Optimum combination with the Sedivette® blood sedimentation system makes it possible to perform
blood sedimentation at any time, immediately following safe obtainment of a blood specimen.
After mixing, measurement starts with insertion of the filled Sedivette® into a vacant measuring
position.
After insertion of the Sedivette® into any available measuring position, the instrument detects the new
Sedivette® and measurement is started. Premature removal of the Sedivette is also detected andindicated as an error.
The measuring times and results of all positions are shown on the display.
The instrument plate is raised and lowered for the measuring procedure. Each Sedivette® isthrough-illuminated by a measuring beam during this process. The beam of light strikes a detector
behind the Sedivette®. The surface of the layer of the erythrocytes is detected by a change in lightintensity.
Correct identification of the specimen is assured by means of the facility for connection of a barcode
reader. The status for each of the forty channels of the basic module and of any expansion modules
can be viewed at any time and is shown on the LCD display.
After completion of measurements, the result record can be outputted via the built-in interface to thecomputer system or printed out via a serial printer, complete with 1h and 2h values (and, optionally,
also ½h and 1h values), date (with two- or four-digit statement of year), time and identity number via
the LCD display.

2.2 The "Blood sedimentation" variable"Blood sedimentation", also referred to as the erythrocyte sedimentation rate (ESR or BSR = (red)blood corpuscle sedimentation rate) can, in accordance with DIN 58935, Part 1, NCCLS H2-A3 andBS 2554, be defined as: The distance (length), in blood rendered non-coagulable, between the liquidsurface of the plasma and the sedimentation surface of the red blood corpuscles stated in mm.
Sedimentation should occur at room temperature in a sedimentation tube of defined height anddefined cross-section. Rate of sedimentation under the influence of gravity is determined after 1 h in
mm using the Westergren method.
For the purpose of performance of blood sedimentation, 2.8 ml of venous blood is absorbed in 0.7 ml
sodium citrate solution in the SARSTEDT S Sedivette®, thoroughly mixed in the S Sedivette®, andplaced vertically in the mounting of the Sediplus® S 2000.
The Sediplus® S 2000 measures the difference in height between the lower meniscus of the liquidsurface of the plasma and the sedimentation surface of the red blood corpuscles. The sedimentation
values of the citrated blood are measured at defined times, converted to mm, and outputted in x mm/h
using the standardized Westergren method after 1 hour. In addition, the Sediplus® S 2000 also makes
it possible to output the sedimentation values after 2 hours in y mm/2h."Page 7

Operating Manual Sediplus® S 2000, V 1.2

2.3 Technical data
Manufacturer's and instrument data
Instrument:
Order number:
Manufacturer:
Address:
Instrument data:
Measuring principle:

Measuring accuracy:
Measuring range:

Display:Interfaces:

Specimen holder:Measuring places:

Measured-data memory:
Data storage:
Data output to:Power supply:Power take-up:
Fuses:
Dimensions:Sediplus® S 2000, 230 V
SARSTEDT No. 90.189 700
SARSTEDT AG & Co.
P.O. Box 1220
D-51582 Nümbrecht, Germany
Infrared transmission measuring process with extraneous lightcompensation
Blood sedimentation measurement in the SARSTEDT Sedivette®
Conversion to Westergren values± 1 mm of measured path
0 to 70 mm, equating to a sedimentation value range of 0 to 147 mm
as per Westergren. Since the Sedivette® has a larger diameter and isshorter than the Westergren sedimentation tube, the sedimentation
value determined is mathematically converted to Westergren values
and displayed by the Sediplus® S 2000.
Measuring time in hours and minutes
Measured value in millimeter (Westergren values)
LCD for status, information and error messages
40 x LED status monitoring of the measuring places
RS 232 (V.24) interface for connection to a printer or acomputer-system. Protocol, see Section: 2.6.4, "Description of serialinterface". Five-pole DIN socket for connection of optional barcode
reader and/or a PC-AT keyboard for data input and/or operation.
25-pole D-SUB interface on rear panel for connection of expansion
modules.
SARSTEDT Sedivette®
Basic module: 40 positions
Expansion module (optional), 40 positions each
A maximum of three expansion modules can be connected to a basic
module.
A memory for a complete record is available for each position.
Data (measured data, date and time) are preserved by means of abuilt-in battery in case of power failure- Display- via RS 232 to a serial printer or a
- computer-based data processing system
230 V~ (-10/+10 %), 50-60 Hz
50 W
0.25 AT (two items fusible cutouts, 5 x 20 mm)
Width: 315 mm
Depth: 326 mm
Height, not inc. Sedivettes: 175 mm (instrument plate retracted)
Height including inserted Sedivettes: 270 mm
5 kg

Weight:Permissible ambient conditions
for storage of the instrument:+ 10° C to +40° C, rel. air humidity max. 80 %, non-condensing
During operation:

Room temperature (18° C to 23° C or as per national or laboratory
specific definition), at max. 80 % rel. air humidity, non-condensing

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Operating Manual Sediplus® S 2000, V 1.2

2.4 Unpacking
The Sediplus® S 2000, 230 V (SARSTEDT No. 90.189 700) or the expansion module for the
Sediplus® S 2000 (SARSTEDT No. 90.189 710) is in each case packed in a carton, complete with allaccessories.
Please check that the carton and the instrument itself are in correct and undamaged
condition. Please contact the carrier immediately in case of any transportation damage!
Please note the notification period for the individual transportation companies (railroad,post, parcels services and freight carriers). This may in some cases be only 24 hours.
Any and all deficiencies and damage must be notified to SARSTEDT AG & Co.
immediately!
Please check the scope of supply using the list provided in the next section.

2.5 Scope of supply
The Sediplus® S 2000, 230 V (SARSTEDT No. 90.189 700) consists of:Quantity1111

Description
Basic module
Mains lead
Anti-dust coverOperating manual

The expansion module for the Sediplus® S 2000 (SARSTEDT No. 90.189 710) consists of:Quantity1
11

Description
Expansion module
Connecting lead for connection to basic
module/expansion module
Anti-dust cover

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Operating Manual Sediplus® S 2000, V 1.2

2.6 Setting-up and installation of the instrument
Attention must under all circumstances be devoted to the following items during the setting-up andinstallation of the instrument, since the BSR can be influenced by numerous sources of error:• Vibration of the filled sedimentation system will cause excessively highsedimentation values.
For this reason the instrument must be positioned on a stable table or anothervibration-free surface.
The instrument must not be positioned on the same laboratory bench as acentrifuge, for example (source of vibration).
• The S-Sedivettes must under no circumstances be scratched, dusty or otherwise
dirty or contaminated.
• Inclination of the filled sedimentation system will cause excessively highsedimentation values.
The instrument must therefore be installed in such a way that the S-Sedivettes are
positioned vertically and the S-Sedivettes can be inserted from above.
• During the measuring operation, the instrument plate moves upward. For thisreason, ensure sufficient space, in order that it is possible to work without
obstruction.
• Deviations and fluctuations (drafts) in room temperature will result in falsification of
the sedimentation values.
For this reason, the instrument must not be installed in the vicinity of radiators orother sources of heat.
• The specimens must not be taken immediately from the refrigerator and then used
for analysis in the S 2000. It is decisive for the correctness of the results that the
specimens are always at room temperature for testing (18° C to 23° C or inaccordance with national or laboratory-specific definition).
• Direct exposure to sunlight or other sources of light must be avoided under all
circumstances.
• A distance of not less than 10 cm from walls and other objects must be maintained,
in order to assure adequate ventilation of the instrument's electronics.
• Always keep the measuring optics scrupulously clean. In particular, no splashes ofblood or plasma may be allowed to enter the optics.
• Protect the instrument against dust and other fouling, etc., by covering it with theanti-dust cover after use.
Connect the instrument to the power supply. The mains plug and mains switch, together
with the fuses, are located on the rear panel of the instrument at the left. The notes onsafety must be read and adhered to before connecting the instrument to the mains.
Ensure adequate spacing from other equipment, in order that there is sufficient spacefor operation and that the function of the instrument is not impaired.
It must be possible to reach and operate the mains switch on the rear of the instrument.
Only switch the mains supply for the Sediplus® S 2000 on once all peripherals (barcode
reader, expansion module(s) and/or PC-AT keyboard) have been correctly connected tothe basic module.

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Operating Manual Sediplus® S 2000, V 1.2

2.6.1 Connection of expansion modules to the Sediplus® S 2000
Important!
The Sediplus® S 2000 basic module must under all circumstances be
disconnected from the power supply when connecting or disconnecting one ormore expansion modules!
A 25-pole D-SUB socket is located on the rear of the Sediplus® S 2000 basic module.
A 25-pole D-SUB socket and a 25-pole D-SUB connector are located in each case on the rear of the
expansion module(s).
A connecting lead (25-pole D-SUB connector/socket) is supplied with each expansion module.

Abbildung 1 Rear view of the basic module (right) and two expansion modules

Please note:
The socket on any unit is always the output and the connector the input of the unit
(Sediplus® S 2000 or expansion module).
This means:
The socket of the basic module must always be connected to the connector of the next
expansion module.
For connection of more than one expansion module, the socket of the first (previous)
expansion module should be connected to the connector of the second (next)
expansion module.

Only use the 25-pole connecting lead supplied with each expansion module for connection of
expansion modules.
The expansion modules are controlled and supplied with power by the basic module. Operation is
also accomplished via the basic module.
A maximum of three expansion modules can be connected to a basic module.

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Operating Manual Sediplus® S 2000, V 1.2

2.6.2 Connection of a PC-AT keyboard and/or a barcode reader
Important!
The Sediplus® S 2000 basic module must under all circumstances be
disconnected from the power supply when connecting or disconnecting one ormore peripherals (barcode reader, PC-AT keyboard, computer system and/or
serial printer)!
Further interfaces for peripherals are located adjacent to the 25-pole D-SUB socket for connection of
expansion modules on the rear of the Sediplus® S 2000:

RS 232 serial interface

Connecting socket forbarcode reader and/or PC
keyboard

Mains switch
Fuses

Mains supply socket

Socket for connection of expansion
modules

Abbildung 2: The rear panel of the Sediplus® S 2000

A barcode reader or a PC-AT keyboard can be connected to the 5-pole DIN connecting
socket, using the adapter.
If you are using the barcode reader for the Sediplus® S 2000, a PC-AT keyboard canalso be used in addition to the barcode reader.
The PC-AT keyboard can in this case be used in parallel to the operating panel and thebarcode reader.

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Operating Manual Sediplus® S 2000, V 1.2
The connections for the three units (S 2000, optional SARSTEDT barcode reader and PC-AT
keyboard) can be seen in the following illustration:
5-pole DIN socket on Sediplus® S 2000

5-pole barcode reader connector
Connecting lead to barcode
reader6-pole connection for PC-AT
keyboard on barcode reader
connector

6-pole connector for PC-AT
keyboard

Please note that the adapter
and connections depend on thebarcode reader and may vary

Abbildung 3: Connection of a keyboard and the optional barcode reader to the Sediplus® S 2000

The PC-AT keyboard can be used immediately after switching-on of the Sediplus® S 2000.
The optional SARSTEDT barcode reader is pre-programmed and initialized for thefollowing codes:- Code 39- Codabar- Code 11- UPC-A/UPC-E- MSI- Code 39 Full ASCII- 2/5 interleaved- Code 128- EAN-8/EAN-11

The reason for a barcode reader not detecting the barcode viewed, reading it
incorrectly or adding characters (e.g. preceding zeros) is often that a different code isused for the barcode (e.g. transmission of check sums).
In this case, it is necessary to reprogram the barcode reader to the code you areusing.
Please check for this purpose the notes and remarks in the operating manualsupplied with the barcode reader.

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Operating Manual Sediplus® S 2000, V 1.2
A plastic mounting is supplied with the optional barcode reader. Please fix this barcode-reader
mounting as follows to the side of the instrument housing:- Firstly remove the double-sided adhesive tape
- Press the mounting against the housing (at the right or the left), as shown in the diagram below- The barcode reader can be inserted into the mounting from above. The mounting serves, on the
one hand, as a storage place and, in addition, the Sedivettes bearing the barcode (adhesive tape)can be held up to the barcode reader before they are inserted into the instrument. The operator's
hands are therefore kept free, and the Sedivette® is vertical during reading of the barcode.
- Remove the barcode reader upward from the mounting if reading off the barcode from a patient's
record sheet

The red LEDs illuminate when the barcode reader is switched on.
- Please see the operating manual supplied with the optional barcode reader for more information on
the barcode reader.

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Operating Manual Sediplus® S 2000, V 1.2

2.6.3 Entry of options
The Sediplus® S 2000 provides a facility for selection of a number of options andparameters prior to use.
The options menu is described in the following sections. Please also read this sectionof the operating manual carefully and select your setting for the various options andparameters.
Switch the instrument on after installing the basic module and peripherals. Keep the [ENTER] key
pressed during this.
The Options menu will appear on the display.
This menu contains eight items; menu item [1] also features a sub-menu. Menu items 5 to 8 can befound on a "new page" on the display. It is possible to leaf between the two "pages" using the arrow
keys.
[1] Output measuring record: Yes[2] Measure at 1h/2h[3] Language: English[4] Reset consecutive ID number[5] Set time/date[6] Move instrument plate up for cleaning[7] Automatic ID No. issue: No[8] Underfilling limit: 05 mm- Use the numerical keys to select the option orparameter which you wish to set or change- Switch the instrument off after editing the parameters
and options- Every change is stored immediately and does notneed to be confirmed (exception: Resetting ofconsecutive ID number)- The modified settings apply when the Sediplus®
S 2000 is switched on again now"Output measuring record" option
A measurement for determination of blood sedimentation values (1h and 2h values) will
normally take two hours (we shall ignore the ½ h/1h option for the time being).

I) Press the [1] numerical key and select "Output measuring record: No".
No output of measuring record via RS 232:
This means that in standard mode, the Sediplus® S 2000 will, after 2 hours have:- determined the 1h and 2h values- mathematically converted the measured data to Westergren values- And provided the values to the user in the list of measurements
The green pilot light at the appurtenant position goes out and the measurement is completed.
Measuring time continues to be counted for checking purposes if the Sedivette® is not immediately
removed from the position. The measured data has been determined at the correct times, however!
Once the Sedivette® has been removed, the measured data is stored until the position in the
instrument is occupied again.
II) Press the [1] numerical key and select "Output measuring record: Yes".
The measuring record is to be outputted via the RS 232:
If the "Output measuring record" option is set to "Yes", the instrument should be connected to thecomputer system or to a serial printer which is ready for operation.
In the case of the Sediplus® S 2000, measurement is only completed in this mode once the measured
data for the respective position has been transmitted to the computer system or to the printer.
This signifies that the Sediplus® S 2000 has, after two hours:- determined the 1h and 2h measured values- mathematically converted the measured data to Westergren values- provided the user with the values in the list of ongoing measurements
The instrument will then attempt to output the data via the serial interface to a printer or to thecomputer system.
- II a) The measuring record can be outputted via the RS 232:
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Operating Manual Sediplus® S 2000, V 1.2-

If this succeeds, measurement is complete.
The Westergren values for the measurements continue to be available to the user
The green pilot lamp on the appurtenant position goes out
The Sedivette® can be removed
The measuring time continues to be counted for checking purposes if the Sedivette® is notremoved from the position immediately. The measured data have been determined at the correct
times, however!
- Once the Sedivette® has been removed, the measuring record remains stored until the position is
occupied again.

- II b) The measuring record cannot be outputted:- If the measuring record cannot be transmitted, the measurement is not complete- The Westergren values for the measurements are available to the user and can be viewed on thedisplay- The green pilot LED on the appurtenant position does not go out- Measurement continues (even if the Westergren values have already been correctly determined)
until the measuring record can be outputted via the RS 232- Removal of the Sedivette® at this point will be detected by the instrument as premature removal ofa Sedivette®
- The instrument issues a corresponding error message and emits a signal tone
- The measurement is completed as soon as the instrument is able to output the measuring record
via the RS 232 (see "II a").
III) Measuring-record options: [2] Date: Output year: Two-digit or Output year: Four-digit
Press the figure "1" until you reach the "Measuring-Record Options" menu. The year contained in
the date of the records can be outputted in two digits or four digits via the serial interface. Press
button "2" in order to select the number of digits for the year:
This is indicated on the display by the message "Output year 2 (4)-digit for date".
*** Measuring record options ***[2] Output year with four digits fordate[3] For measured value: Output >symbol-> Close using ESC*** Measuring record options ***[2] Output year with two digits for date[3] For measured value: Output > symbol-> Close using ESC

This setting must under all circumstances be taken into account for connection of the
instrument to the laboratory or hospital computer system. Consult your systemadministrator in case of doubt.
IV) Measuring-record option "Output measured value with ">" symbol"
Due to the optical measuring principle used, the Sediplus® S 2000 detects the column of blood up to
the maximum of the label of the Sedivette®. Sedimentation lengths which are larger than the areascanned cannot be detected by the instrument.
The blood may be able to sediment further behind the label during the measuring period.
For this reason, the Sediplus® S 2000 has been expanded with a new function as from Version 1.9.
As from Version 1.9, the instrument detects whether the measuring-range limit is reached or
exceeded. The S 2000 indicates this by means of a ">" symbol prefixing the measured value.
The measured value actually determined (zero measurement minus the height of the start of thelabel) is indicated as the measured value.
The ">" symbol continues to be displayed in all cases for the 2h measured value for plausibility
reasons if a measuring-range overshoot is ascertained in the case of the 1h measured value.
In order to keep the measuring record outputted via the RS 232 interface compatible
with preceding versions, it is possible to suppress output of the ">" symbol via theserial interface. Selection can be accomplished using the "Measuring-Record Options"menu of the S 2000.

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Operating Manual Sediplus® S 2000, V 1.2
Press button [1] twice in order to access the "Measuring-Record Options" sub-menu of the "Output
measuring record" item:[1] Output measuring record: Yes[2] Measurement at: 1h/2h[3] Language: English[4] Reset consecutive ID No.

Press button [3] in order to output the measured data with or without the ">" symbol:*** Measuring record options ***[2] Output year with four digits for date[3] For measured value: No output > symbol-> Close using ESC*** Measuring record options ***[2] Output year with four digits for date[3] For measured value: No output > symbol-> Close using ESC

Close the sub-menu by pressing the [ESC] key.
For safety's sake, please check the settings for Language, Underfilling limit, Serialinterface output Yes/No, Reset consecutive ID number or not and 1h or 2hmeasurement.
If necessary, set the values you require again (see Section 2.6.3, "Entry of options").

2.6.3.2 "Measurement at ½h/1h" option
It is possible, using the Sediplus® S 2000, to determine and output the ½h and 1h measured values
instead of the 1h and 2h measured values. Operation of the instrument is not altered by this selection.
The measured results are displayed as the ½h/1h values, however. The measurement operation for a
specimen is completed after one hour if the "½h and 1h" setting is selected.
Press numerical key [2]. The instrument will switch to the "½h/1h" mode. The instrument switches
back to the 1h/2h mode when the numerical key [2] is pressed again.
The settings are stored when the instrument is switched off.

2.6.3.3 "Language" option
Like the options described already, "Language", too, can also be altered by pressing the numericalbutton.
Button [3] can be used to toggle between German and English text output. Reconfiguration to an
external PC-AT keyboard with a US character set is installed automatically when English is selected
as the language.

2.6.3.4 "Reset consecutive identification number" option
Press button [4] in order to reset the identification number. The current identification number, e.g. "ID:0000082344, the "The identification number will now be reset" message and → "Press key to confirm"
will appear on the display. Press any key to reset the identification number to the initial value 00...1.
This procedure can be aborted using [ESC].

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Operating Manual Sediplus® S 2000, V 1.2

2.6.3.5 "Set time/date" option
The Sediplus® S 2000 has a built-in electronic clock.
This menu makes it possible to set the time, date and day of the week.
The date, time and day of the week are default set at the manufacturer's works.
Pressing numerical button [5] in the Option menu accesses the menu for entry of thetime, day of the week and date:*** Enter time and date ***Hour: 11 → Value: 0 to 23

Use the numerical band to enter the required data and confirm your entry by pressing the [ENTER] ineach case. The cursor will then move on to the next box.
The following settings are possible:
Hour
Minute
Day
Month
Year
Day of week:[ENTER][ENTER][ENTER][ENTER][ENTER]
Use [Ident] to select the day of the week and then use [ESC] to close this menu.
It is possible to select between two-digit (04) and four-digit (2004) formats for the
display of the year.

2.6.3.6 "Raise instrument plate for cleaning" function
Pressing of button [6] raises the instrument plate automatically to permit cleaning of the
instrument. The instrument plate remains in this position for 120 s. The message "S 2000 Cleaning
Position" appears on the instrument's display. In addition, the remaining time is counted down in thefollowing message: "The instrument plate will move down again automatically in 120 s". This process
can be aborted at any time using [ESC].

2.6.3.7 "Automatic issue of consecutive identification number: Yes/No"
function
Consecutive identification number:
A patient or specimen identification number must be issued for every measurement. This
number can be entered in various ways:- Using the touch-type keyboard on the Sediplus® S 2000
- By means of an optional barcode reader (if purchased)- By means of a connected PC-AT keyboard (if available)- In addition to the above possibilities, the instrument can also suggest a consecutive
identification number
If the identification number suggested is accepted, the instrument increases it by one automatically at
the start of every new measurement.
The "Automatic issue of consecutive identification number" menu item can be found in the Options
menu.
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Operating Manual Sediplus® S 2000, V 1.2
This function can be set to "Yes" or "No" using numerical key [7].
With the function set to "No", the identification number can be entered as described in Section 4.4,
"Entry of identification number".
If the function is set to "Yes", the next consecutive identification number for the Sedivette® is issued
automatically when the Sedivette® is inserted (without previous pressing of the [IDENT] button orreading of a barcode).

2.6.3.8 "Underfilling limit 5 to 15 mm" function
The "Underfilling limit 5 mm" menu item can be found in the Options menu.
This option is used to define in millimeters the limit, up to which underfilling in the Sedivettes is not to
be detected as an error by the Sediplus® S 2000 (please also see Section 5.2, "Underfilling of the
S-Sedivette®"). This limit can be set to values of between 5 and 15 mm.
Example:A value of 8 mm is selected for this option:- The measurement/the measured data will not be identified as "underfilled" on the result record if the
Sedivette® is filled only up to a point 8 mm (or less) below the widening-point in the neck of the
Sedivette.
- The measurement/the measured data will be identified as "underfilled" on the result record if the
Sedivette® is filled only up to a point 8 mm (or more) below the widening-point of the neck of the
Sedivette.
- Assessment of the data obtained from underfilled Sedivettes is at the discretion of the operating
staff, the laboratory management or the responsible physician. The reason for this is the differing
sedimentation behaviour when Sedivettes are filled to differing extents.
- The Sedivettes are correctly filled provided the plunger is fully extended.
- Ensure when breaking off the plunger that it does not get thrust back into the Sedivette® a short way
again.
- The blood level in the Sedivette® should extend up to the start of the widening-point in the neck of
the Sedivette®.
Numerous tests have demonstrated that underfilling of up to 5 mm below the widening
point of the neck of the Sedivette produces tolerable results.
Any change in the limit is at the discretion of the operating staff, the laboratory
management or the responsible doctor. The same applies to the assessment of themeasured data obtained using "underfilled" Sedivettes.
Please note when assessing measured data that the maximum measuring length which
can be evaluated extends from the meniscus of the column of blood up to the top edgeof the label.
The effective measuring range for Sedivettes containing a smaller initial volume istherefore shortened by enlarging the "underfilling limit" (see Section 5.2 "Underfilling of
the S-Sedivette®").
Setting of the required limit:
Repeated pressing of button [8] increases the limit by one millimeter in each case. The available
selection restarts at a value of 5 mm once the 15 mm extreme limit has been reached. The limit
selected is displayed to the right of line "[8] Underfilling limit:"
↑ [5]
[6]
[7]
[8]

Set time/date↑Raise measuring arm for cleaning
Automatic issue of consecutive ID No.: No
Underfilling limit: 5 mm

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Operating Manual Sediplus® S 2000, V 1.2

2.6.4 Description of the serial interface
The Sediplus® S 2000 is equipped with a serial interface for connection of a serial printer or alaboratory computer system.
Only one connection to the laboratory computer system or to the printer is needed,even if work is being performed using multiple expansion modules.
The "Output measuring record" option must be set to "Yes" if you wish to output themeasuring records via the serial interface after measurement.
Please see on this subject the detailed description contained in Section 2.6.3.1,"Output measuring record" option.
The complete record of the measurement is outputted via the serial interface as soon
as the Sediplus® S 2000 has determined the 1h and 2h (or, optionally, the ½h and 1h)
values for blood sedimentation for one position.
If you are using the optional SARSTEDT printer, it must under all circumstances beensured that the Sediplus® S 2000 is switched on first, followed only then by the serial
printer. Communication problems may otherwise occur, with the printer printing only
special characters..
a) Description of interface (important for connection to the laboratory computer system):
The RS 232 C standard, corresponding to DIN 66259, Part 1, applies to the electrical properties of theinterface signals and for assignment of signal states.
Character format:
ASCII format

18Start bit
Data bits

None
1

Parity bit
Stop bit

Transmission rate:
9600 Baud (Bit/s)

Data flow control:
Hardware handshake DSR

Data flow between the Sediplus® S 2000 and the laboratory computer system is controlled by meansof hardware handshake. This means that the Sediplus® S 2000 expects at its DSR input (Pin 6: DSR
input on 9-pole D-SUB connector of S 2000) the DTR (Data Transmission Ready) signal or the RTS(Request To Send) signal from the laboratory computer system.
Once the corresponding signal is received, data transmission can be controlled from the computer
system.
The question of which of the two signals (DTR or RTS) is ultimately used depends on the
programming of the computer system and on the cable connection between the S 2000 and thecomputer system.
b) Connector (pin) assignment on the Sediplus® S 2000:
9-pole D-SUB connector in accordance with IBM standard
Pin 1 and 2:
Pin 3:
Pin 4:
Pin 5:Pin 6:
Pin 7 to 9:

not assigned
TxD (output)
not assigned
GND
DSR (input)
not assigned®

The Sediplus S 2000 expects at its DSR
input (Pin 6) the DTR (Data Transmission
ready) or RTS (Request To Send) signal
from the laboratory computer system.

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Operating Manual Sediplus® S 2000, V 1.2c) Interface protocol:
The data for the measuring record is transmitted block-by-block in ASCII code via the RS 232. Everydata block consists of a start character, the measuring record and the end character.
- The control character STX (start of text) is transmitted at the start- The ETX (end of text) character is transmitted at the end- Every print-line is terminated with a CR/LF.
Example: (ID Number = 12345 (max. 36 characters); 1h value = 1 mm; 2h value = 10 mm)
STX control character (start of text) / ASCII 2

In the "Year Two-Digit" option, the format isshown as in the example on the left. In the"Year Four-Digit" option, the year istransmitted in four-digit form. The remaining
data in the line is shifted by two places.

STX
27.10.03, 11:52 UhrModul: 1 position: 12 U
ID: 12345
1h-value: 1 mm P
2h-value: 10 mm
ETX

1 x "space" & "U" in case of underfilling of the®
Sedivette
The identification number terminates with a
CR/LF
1 x "space" 6 "P" in case of error due to lack of®
Sedivette or power failure at time of
measurement.
ETX control character (end of text) / ASCII 3

Always use a high-quality interface connector cable featuring the following
properties to connect the Sediplus® S 2000 to a printer or to the computer system, inorder to meet the requirements of the Electromagnetic Compatibility Directive:
Screening: Lead with screening braid. The braid must be applied flatly on the
shrouding collar.
Shrouding: The connector/socket shrouding must be of solid metal.
Length: Use the lead supplied for the printer (or a comparable lead with a maximum
length of 1.80 m).
Diagrams of the pin assignments of these leads can be found in the Annex.

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Operating Manual Sediplus® S 2000, V 1.2

3 The functional elements of the Sediplus® S 2000
Guide holes for the®
Sedivette®

Sedivette
insertedControl LEDs
Instrument
plate

Operating panel

Display

Abbildung 4 Front view of the Sediplus® S 2000

3.1 The instrument plate
The instrument plate features forty guide holes for the Sedivettes, each with a green pilot LED. Eachof the forty guide holes, each with a pilot LED, corresponds to a position for performance of ameasurement.
The Sedivettes are inserted vertically into the mountings of the basic module via the guide holes.
The sensor system for measurement of sedimentation is housed in the instrument plate.
The movement (upward and downward movement during measurement) of the instrument plate must
therefore not be obstructed.
Use only SARSTEDT Sedivette® as the patient's blood carrier for the Sediplus®
S 2000.
Other tubes, such as Westergren tubes, for example, will produce incorrect
blood sedimentation readings.

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Operating Manual Sediplus® S 2000, V 1.2

3.2 The LEDs on the instrument plate
The LEDs indicate the various states of the appurtenant position or of the
instrument as a whole.
All 40 LEDs flash simultaneously.
Visual signal: The instrument plate will move upward and down again in the next 5 seconds for the
purpose of measurement. No Sedivettes may be inserted during this movement period. Wait until themeasuring sequence has been completed, then insert the Sedivettes.
The LED for a specific position is off and the appurtenant position is not occupied.
This position is vacant. A Sedivette® can be inserted for the purpose of measurement.
The LED of a position is on. The position is occupied by a Sedivette®.
Measurement using the Sedivette® inserted has not yet been completed.
The current status of this position can be viewed on the display.
The LED of an occupied position is off.
Measurement has been completed. The result can be viewed on the display. The Sedivette® can beremoved from the instrument. The measuring position thus becomes vacant for the next
measurement.
The LED for a position is flashing but the position is not occupied.
The Sedivette® which was in this position has been removed before completion of a measurement.
The following warning message appears on the display:Mod. 1 Pos. 7: This is the position
at which an error has occurred.
Mod. 1 = Module 1 (basic module)Pos. 7 = Position 7

Indication: Malfunction®

The Sedivette has been removed

IMPORTANT! Mod. 1 Pos. 7 has been removed!
Re-insert the SEDIVETTE or abort measurement using[DEL]

DISPLAY

IDENT

ESC

7

8

9

4
1
0

5
2

6
3

DEL

↵

Rectify problem. Select one of the methods
suggested for this purpose.

- The position will be in one of the states described above once the error/fault/malfunction has been
rectified, using one of the two methods- If more than one LEDs were flashing previously, check the error message for the next position
affected on the display

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Operating Manual Sediplus® S 2000, V 1.2

3.3 The display
The display makes it possible to view all measured data for completed and ongoing measurements,using the operating panel. Instrument status (date, day of week, time, number of modules, number ofvacant and number of occupied positions and number of measurements completed) is outputted via
the display. The following illustration shows the status of the display after switching-on:

Total number of vacant
positions (on all modules).

Date and time
Number of occupied
positions. This figure iscontinuously updated.

Total number ofvacant positions (onall modules).
SARSTEDT S 2000 08:18 on Mo. 11.10.2004
1 module 40 positions vacant 0 occupied
0 measurements completed→ Press IDENT to start measurement

DISPLAY

IDENT

7

8

9

4

5

6

1

2

3

Note concerning operation
ESC
and procedure(alwaysdisplayed)

DEL

The [DISPLAY] key can be used (provided the corresponding data is present) to toggle between thelist of ongoing measurements (or positions from which the Sedivette® has not yet been removed),
completed measurements (or positions from which the Sedivette® has been removed) and the status
display (condition of the display after switching-on).
The [\/] and [/\] keys can be used to display the results of various positions.
Use the numerical keys on the operating panel to toggle between the records of various modules if
more than one module is in operation.

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Operating Manual Sediplus® S 2000, V 1.2

4 Operation of the Sediplus® S 2000
4.1 Switching on the instrument
Use the mains switch to activate the Sediplus® S 2000. The following display will appear. This
contains the version number of the software in the EPROM.
***** SARSTEDT ********** AG & CO. *****SEDIPLUS S 2000 <====> VERSION X.X

The instrument switches onward automatically after a few seconds. The status display will appear.
The instrument is then immediately ready for operation.
*SARSTEDT S 2000* 08:18 on Mo. 11.10.2004
1 module 40 positions vacant 0 occupied
0 measurements completed→ Press IDENT to start measurement

4.2 Preparation of specimens
Please also note the disruptive factors (e.g. paraproteinaemia, lipaemia, haemolysis)
described in the medical literature which may have an influence on measurement ofblood sedimentation, and check the plausibility of the test result in case of any suspectblood specimens.
• Only use S-Sedivettes for the SARSTEDT Sediplus® S 2000 blood
sedimentation system• The surface of the S-Sedivettes must be clean. There must be no remnants oflabels or traces of powder (from gloves) on this surface. Such fouling would
cause additional signals which are registered and which could significantly impair
determination of sedimentation. The original label and labels applied by users at
the same height as the original label do not affect measurement.
• It must in addition be ensured that there are no droplets of blood in the cap andthat the cone in the S-Sedivette® is not wetted during insertion. This can occur
after taking of a blood sample and/or after mixing. The measurement could befalsified after starting as a result of dripping of blood.
Safety-S-Sedivette® for taking of blood samples
Use SARSTEDT S-Sedivette® with sodium citrate solution as an anti-coagulant for taking of blood
samples.
Please note under all circumstances the notes on use and handling in the
SARSTEDT S-Sedivette® pack

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Operating Manual Sediplus® S 2000, V 1.2
The S-Sedivette® is a sealed blood-sampling system which is used simultaneously as thesedimentation tube. There is no need for decanting of blood or transfer of blood to a separate pipette.
Important!
Every blood sample must in every case be mixed thoroughly but without generation offoam immediately after having been taken and immediately prior to insertion into the
instrument.
Such mixing can be performed manually, in such a way that the mixing ball containedin the Sedivette® passes through the complete blood specimen or gently andconveniently using a machine specially developed by SARSTEDT AG & Co. for thispurpose, the Sarmix® M 200 (see Section 9, "Information for ordering").
Use the SARSTEDT Sarmix® M 200 for careful and gentle mixing. This mixer makes it
possible to mix four S-Sedivettes® simultaneously in eight mixing cycles with alternatinghigh and low mixing speeds.
Keeping of blood specimens:
Wherever possible, use the blood specimen obtained immediately. Analysis should be
performed within 2 h if the specimen is stored at room temperature.
The blood specimen must not be coagulated.

4.3 Insertion of the Sedivette® and starting of a measurement
The identification number can be entered, the Sedivette® inserted and measurement started invarious ways (or in various orders).
The following sequence applies in principle to all types of insertion:- Measurement starts automatically once the Sedivettes have been inserted.
- All LEDs start flashing 20 seconds after the final Sedivette® has been inserted. No further Sedivettes
may now be inserted.
- After a further 5 seconds, the instrument plate scans the newly inserted Sedivette(s)®.
- In this case, scanning of the Sedivettes starts 25 seconds after insertion of the final Sedivette®.
The Sedivettes inserted will in every case be scanned not later than two minutes after
insertion of the final Sedivette®.
- After scanning, the display will show the status view with information on the number of modules,
occupied and vacant positions, and how many measurements have been completed. New
Sedivettes can now be inserted and further measurements thus started.
Method 1:
The barcode or patient's identification number is located on the Sedivette®. This must
therefore be entered before the Sedivette® is inserted.
- Press the button [IDENT] on the operating panel (as described on the display). The following display
will appear:*** Start sedimentation ***ID: 0000000001
Please enter an ID number or confirm thenumber shown using IDENT- Enter the identification number:
The identification number can be entered via the operating panel or using an optional PC-AT
keyboard and confirmed using the [ENTER] key. Another possibility is that of accepting (confirming)
the suggested identification number by pressing the [IDENT] key.

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Operating Manual Sediplus® S 2000, V 1.2- The following display will appear:*** Start sedimentation ***ID: New Identification Number
Now insert the Sedivette into a vacant
position. (Abort using ESC)

- Insert one or more Sedivettes into any vacant positions.
Method 2:
The barcode or patient identification number is only available separately (i.e., not on the
Sedivette®).
- If the patient's identification number is not located on the Sedivette®, the Sedivettes can be inserted
into vacant measuring positions without previously pressing a button.
- Measurement starts automatically upon insertion. The following display will appear:
IMPORTANT. Mod. 1 Pos. 14 has no ID!
ID: 0000000001
Please enter an ID number of confirm thesuggested number using IDENT

Method 2:
The barcode or patient identification number is only available separately (i.e., not on the
Sedivette®).
- If the patient's identification number is not located on the Sedivette®, the Sedivettes can be inserted
into vacant measuring positions without previously pressing a button.
- Measurement starts automatically upon insertion.
The following display will appear:*SARSTEDT S 2000 08:18 on Mo, 11.10.2004
1 module 39 positions vacant 1 occupied
0 measurements completedpress IDENT to start measurement

Method 3:
Using an optional barcode reader to enter the patient's identification number.
- Hold the barcode reader up to the barcode for the Sedivette® (on the Sedivette®, for example, or on
the patient's record sheet) without previously pressing any button on the control panel and without
inserting the Sedivette® into a vacant position.
- If no error is on at this time (as a result of a Sedivette® being removed prematurely, for example), thebarcode is automatically recognized and the following message will appear on the display:*** Start sedimentation ***ID: BARCODE READ
Please now insert the SEDIVETTE into avacant position.
(Abortusing ESC)

- Insert the Sedivette® into any vacant position-The status display will appear

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Operating Manual Sediplus® S 2000, V 1.2

4.4 Entry of the identification number
As described in the previous section, there are various ways of entering the identification number.
This can, on the one hand, be accomplished on request using the operating panel, or by means of abarcode reader or a PC keyboard (option).
The Sediplus® S 2000 also makes it possible to issue the same identification number multiple times(Danger of incorrect identification!). In such a case, differentiation is possible only on the basis of
position, module or time!
- The instrument suggests a consecutive identification number when the [IDENT] button is pressed orwhen a Sedivette® is inserted.
- This number, if confirmed, is increased by one automatically by the instrument in each case witheach succeeding measurement.
- This (suggested) consecutive number can be confirmed as the identification number by pressing the[IDENT] button.
- It is also possible to enter one's own identification number.
- For this purpose, use the numerical keys from "1" to "0".
- The suggested identification number can be erased/overtyped by entering the first numeral.
- Further numerals can be entered or the last numeral in each case erased using the [DEL] key.
- Confirm the entry using the [ENTER] key.
- The procedure is analogous if a keyboard is used.

4.4.1 Entry of the identification number using a barcode reader (optional)
A further method of entering the identification number isthat of scanning the number using an optional barcode
reader (in many cases, the patient's identification number ispresent on the Sedivette® or on a patient's record sheet in
the form of a barcode).
Always be sure to hold the Sedivette® vertical whenreading the barcode off from it. Move the barcode on the
Sedivette® along the fixed barcode reader (see Section
2.6.2, "Connection of a PC-AT keyboard and/or a barcode
reader" or Section 16, "Annex" for information on
installation of the optional barcode reader).
Pick up the barcode reader and move it over the barcodeon the patient's record sheet.
Please adhere to the following angles and distances whenreading off barcodes from the Sedivette® or the patient's
record sheet.

Always hold the
Sedivette® vertical!

Move the barcode reader over the barcode.
Keep it at a distance of 0 to 1 cm.

Distance: 0 to 10 mm

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Operating Manual Sediplus® S 2000, V 1.2

Vertical angle: 0 to 10°
Maintain a vertical angle of 0 to 10°.
.

This angle must not be greater than 10° when
the barcode reader is tilted forward.
Front angle: max. 10°°

This angle must not be greater than 10°
when the barcode reader is tilted backward.

Rear angle: max. 10°°- If the barcode is not read immediately, point the barcode reader in a different direction for a secondor two or hold it against a different object (e.g. text on the patient's record sheet). Then try again toread the required barcode.
- If an error or other message is on the display, e.g.:
Important! Mod. 1 Pos. 7 has been
removed!

or:Please replace the SEDIVETTE or abortmeasurement using DEL!

*** Instrument plate has been
obstructed! ***Please remove the obstruction!
Confirm by pressing key

and an attempt is being made to read a barcode with the barcode reader, the instrument willrecognize the barcode but not display it.
The cause of the error must firstly be eliminated before the instrument indicates that it hasrecognized a barcode. It is then possible to start measurement or abort using [ESC].

The S 2000 has received the following
BARCODE
ID: BARCODE READ
Please now insert the SEDIVETTE into avacant position.
(Abort using ESC)DISPLAY

Please replace the SEDIVETTE orabort measurement using DEL!.

IDENT

ESC

7

8

9

4

5

6

1

2

3

0

DEL

↵

The [ESC] key on the operating panel canbe used to abort the entry. The display thenreverts to the status display.

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Operating Manual Sediplus® S 2000, V 1.2

4.4.2 Entry of the identification number using a PC-AT keyboard (optional)
It is possible to connect a PC-AT keyboard to the 5-pole connecting socket for the barcode reader(please also see Section 2.6.2, "Connection of a PC-AT keyboard and/or a barcode reader").
The PC-AT keyboard can be used in parallel with the operating panel and the barcode reader.
The following table indicates which keys on the Sediplus® S 2000's operating panel (and therefore
which functions) correspond to the keys on the PC-AT keyboard:®

Keys on the Sediplus S 2000

Keys on the PC-AT keyboard
Cursor key (arrow up)

Cursor key (arrow down)

Cursor keys
(arrow right and arrow left)

Display[ENTER] key
ENTER

IDENT
[ESC] key

ESC

ESC
[ENTER] key

↵

ENTER
"Delete" or "Backspace" key

DEL

Entf

Numerical keys from [1] to [0]

1

1

0

The PC-AT keyboard can be used toassign letters for the identification
number. This is not possible using the®
Sediplus S 2000's operating panel.

0

The remaining keys can be used for entry of letters and special
characters ([SHIFT] & "Character" only) for the identification number.
A US keyboard (differing key assignment) can be connected if English
is selected as the language.

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Operating Manual Sediplus® S 2000, V 1.2

4.5 Viewing of values already determined during a measurement
Determination of measured data for one position is completed after one hour (½h/1h option) or twohours (1h/2h option), depending on the option selected. The two sedimentation values for the position
are then available.
It is possible to view the values already determined on the display prior to completion of
measurement. Only measured data converted to Westergren values is displayed.
Example:The status display is shown during a measurement. It indicates the number of occupied positions andthe number of measurements completed from this number.
*SARSTEDT S 2000 08:18 on Mo, 18.08.2004
1 module 38 positions vacant 2 occupied
0 measurements completed
Press IDENT to start measurement- A list of the measurements which have not yet been completed appears if the [DISPLAY] button ispressed once.
In the following example, a Sedivette® with identification number 000000002 has been in (M.)
Module 1 at (Pos.) Position 9 for 1 hour and 10 minutes. The 1h value of 5 mm is available. No value
is available as yet for (Pos.) Position 10 in (M.) Module 1, since measurement has been progressing
here only for 5 minutes.
M. Pos. ID number 1h value 2h value Time1
9 000000002
5mm-01:10
1 10 000000003--00:05- Use the arrow keys [\/] and [/\] to scroll up and down the display for viewing of other measurements
if more than three measurements are being performed.
The numerical keys on the touch-type keyboard can be used to access the records of other modules if
further modules are connected to the basic module. Press:- Key [1] for the records in the basic module- Key [2] for the records in the first expansion module- etc..
Switching of the display has no effect on the measuring operation.
- There are in principle two possibilities if the [DISPLAY] key is pressed again:a) No measurements as yet completed:
Positions at which a measurement has, however, been completed at an earlier time have alreadybeen re-occupied with a Sedivette® (the records of these previous measurements have beenerased from the memory).
- In this case, the status display will appear if the [DISPLAY] key is pressed again- The measured data for completed measurements, the positions of which have been re-allocated, isno longer available- Continue with measurementsb) Measurements have been completed:
Positions at which a measurement has been completed have not yet been re-occupied with a
Sedivette® (the records of these measurements have not yet been erased from the memory).
- If the [DISPLAY] key is pressed a second time in this case, the measuring records can be viewed,
complete with the module, position and identification number of the Sedivettes removed, and therelevant sedimentation values (provided the position in the instrument has not yet been
re-occupied).
- The instrument indicates removed Sedivettes on the display as follows:*** Values for removed SEDIVETTES ***-> Sedimentation values: Module 1/Position 1
ID: 0000000001
1h value: 3mm
2h value
5mm

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Operating Manual Sediplus® S 2000, V 1.2- The arrow keys can be used to access the records for further Sedivettes which have already been
removed- The numerical keys can be used to access the records of Sedivettes which have already been
removed from other modules (if any other modules are connected)- The status display appears if the [DISPLAY] key is pressed again
There are three ways of accessing the measuring record once a measurement has been completed.
a) Before removing the Sedivette®:- It is possible to press the [DISPLAY] key and move to the list of measurements
The measuring record will be found in this list (provided the Sedivette® has not yet been removed).
The time figure under "Time" will continue to be counted.
- The same procedure can be selected in order to view the measured data for measurements not yet
completedb) The display if a Sedivette® has been removed:- The instrument switches the display and shows the measuring record for 15 seconds at the moment
at which the Sedivette® is removed- The measuring record will then be erased from the list of measurements- It is possible to view the result in the "Values for Sedivettes® removed" list, however.
c) Measuring record after removal of a Sedivette®:- Use the [DISPLAY] key to move to the "Values for removed Sedivettes®" list
- Browse the values for the various Sedivettes removed using the arrow keys
Important!
When a new Sedivette® is inserted into a position, the measuring record for the
Sedivette® previously removed from this position is erased immediately!
The data is no longer available!

4.6 Acoustic assistance
The Sediplus® S 2000 features acoustic assistance for operation. It is necessary todifferentiate between:a single tone andtwo short tones in quick succession
The single tone is always used as a warning signal, as in the following cases, for example:• When a Sedivette® has been inserted but the identification number has not yet been entered• If a Sedivette® is removed before completion of measurement• If the [DEL] key is pressed and the instrument switched on, in order to erase ongoing measurements• If the instrument plate has been obstructed during downward movement
The double tone indicates confirmation of the identification number entered. This is the case:• if the [ENTER] key is pressed after the identification number has been entered• if the consecutive identification number is confirmed using the [IDENT] key• if a valid barcode has been recognized using the optional barcode reader

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4.7 Erasure of all data
It is not possible to reverse this function!
Performance of this function will erase all data for both completed anduncompleted measurements.
- Switch the instrument off- Press the [DEL] key on the operating panel- Switch the instrument on, keeping the [DEL] key pressed while doing this
- The instrument will switch to the following display:
All ongoing measurements to be erased
Confirm by pressing key- Press the [ESC] key in order to cancel erasure of the ongoing measurements
Pressing of any other key will result in erasure of the ongoing measurements.
Any Sedivettes in the instrument will be detected as newly inserted Sedivettes by the Sediplus®
S 2000 after the erasure operation.
The instrument scans the identification numbers of all Sedivettes and starts the measurements.

4.8 Renewed print-out / Repeat print-out operation
If the "Output measuring record: Yes/No" option was set to "Yes" prior to the start of measurements, itis then possible to transfer certain measured data/records to the computer system or to output themagain on the serial printer connected.
This applies only to the records for those positions:- the measurement of which has already been completed (i.e., the Sediplus® S 2000 has determinedboth sedimentation values for this position and has outputted the appurtenant record via the serialinterface)- and from which the Sedivette® has not yet been removed
Press both arrow keys [\/] and [/\] simultaneously. All the records for completed measurements will be
printed out again provided the appurtenant Sedivettes have not yet been removed. The appurtenant
record can no longer be transmitted once the Sedivette® has been removed from its position.

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5 Malfunctions and operating errors
Please also note the disrupting factors described in medical literature (e.g.
paraproteinaemia, lipaemia, and haemolysis), which may have an influence on
measurement of blood sedimentation, and check the plausibility of the results obtained incase of suspect blood specimens.

5.1 Obstruction of instrument plate's upward and downward movement
The instrument plate must not be obstructed during its upward or downward
movement. There must be no objects on or next to the instrument plate. If downward
movement is obstructed for any reason, the instrument plate will move up again andremain motionless. The LEDs will flash and the following error message will appear:*** Instrument plate has been
obstructed!***Please remove obstruction.
.-> Confirm by pressing key

Remove the obstructive objects and press any key.
The measuring arm will move to the starting position. Measurement will be repeated after a few
seconds.
If the instrument plate is obstructed while moving upward, it will return to the starting position after a
few seconds. In this case, there is no message on the display. The obstructed measurement isrepeated after 20 seconds.

5.2 Underfilling of the S-Sedivette®
The Sedivettes must be correctly filled for measurement of blood sedimentation. It
must therefore be ensured as early as the blood-sampling stage that the SARSTEDT
Sedivettes are correctly used and handled (see Notes on Use in the Sedivette pack).
- The Sedivettes are correctly filled provided the plunger is fully drawn out and notthrust back a distance into the Sedivette® during breaking off of the plunger stem.
- The blood level in the Sedivette® should reach up to the start of thewidening-point of the neck of the Sedivette- In case of underfilling, the operator must decide at his discretion whether the
BSR/ESR values are reliable- The volume of blood in the column in the Sedivette has an effect on thesedimentation value
The instrument detects whether the Sedivette is underfilled at the first measurement. The height fromwhich underfilling is detected as an error and recorded can be specified in the Options menu (Section
2.6.3.8, "Underfilling limit 5 to 15 mm function"). An underfilled Sedivette® is recorded and indicatedon the display.

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Sedimentation values as a function of filling height
P 0:

P0
P1
P2
P3

P 1:

P 2:
P 3:

Range P 1 to P 3:

P 4:

"Cylindrical-to-tapering" transition point,corresponds to filling level at 50 mbar venous
pressure
5 mm below P 0
A Sedivette® filled above this point is notmarked with an "U" to indicate "underfilled"
10 mm below P 0
15 mm below P 0
Below this point, a Sedivette® is always
marked with a "U" to indicate "underfilled"
Possible setting range for users of the S 2000
software for marking of the Sedivette® with a"U" for "underfilled"
Upper edge of label
End of usable measuring window

P4
Absolute distances, mm
P 0 to P 4
P 1 to P 4
P 2 to P 4
P 3 to P 4

70 mm
65 mm
60 mm
55 mm

corresponding toWestergren, mm
147 mm
116 mm99 mm
86 mm

In the list of ongoing measurements (accessible using the [DISPLAY] key):
The first measurement is performed a few seconds after insertion of the Sedivette®. The instrument
has detected underfilling of the Sedivette® even at this point.
- Pressing of the [DISPLAY] key causes the instrument to display the list of ongoing measurements- In case of underfilling, a white "U" is displayed on a dark background after the position number. This"U" remains visible throughout the period of the measurement.
M. Pos ID-Nummer
1 15U0000000009
1 16U0000000010
1 17U0000000011

1h-Wert 2h-Wert Dauer
23mm
25mm 02:05
10mm-00:30--00:01

On a display, if a Sedivette® has been removed after measurement:
When a Sedivette® is removed after completion of measurement, the sedimentation values for thisSedivette® will appear immediately on the screen.
On this display, too, a white "U" is shown on a dark background (in case of underfilling) after the
position number.
Important: This display of the measuring record remains visible only for 15 seconds. The
sedimentation values for this position can then be found in the "Values for Sedivettes removed" list.
-> SEDIMENTATION VALUES: Module 1 / Position 10U
ID: 0000000015
1h value: 21mm
2h value: 30mm
Sedimentation time: 2 hours, 5 minutes

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Operating Manual Sediplus® S 2000, V 1.2"Values for Sedivettes removed" list:
The measured data from Sedivettes which have been removed can also be viewed at a later time.
This is possible only as long as no new Sedivette® has been inserted into the relevant position.
The "Values for Sedivettes removed" list can be accessed using the [DISPLAY] key. Use the arrow
keys to leaf through the values for the individual positions in this list.
The numerical keys can be used to switch to record lists for other modules (where other modules are
installed).
In this display, too, a light "U" in a dark box after the position number indicates a case of underfilling.
*** Values for SEDIVETTES removed ***-> SEDIMENTATION VALUES: Module 1 / Position 10U
ID: 0000000015
1h VALUE: 21mm
2h VALUE:
30mm

5.3 Incorrect measurement
Please note:A negative value is outputted if the value determined for a measurement is greaterthan that registered for the zero measurement. The measured value is prefixed witha minus sign.
A negative value can only occur as a result of an incorrect measurement.
Check the Sedivette® for correct filling level and for external damage (fouling,
scratches, etc.). Then repeat the measurement. Please contact the DESAGA GmbH
Service Department at Wiesloch or your local SARSTEDT branch or office if theerror persists.

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5.4 Premature removal of a Sedivette®
Premature removal of a Sedivette® is treated by the instrument as an error.
This error occurs if a Sedivette® is removed from its position but the measurement
has not yet been completed (please also see Section 4.3, "Insertion of the Sedivette®
and starting of measurement").
Cases in which the option "Output measuring record:" has been set to "Yes" but it has not beenpossible to send any data to the computer system or to a printer are also evaluated as premature
removal of a Sedivette®. A warning message is also given in this case.
When the Sedivette® is re-inserted, measurement is continued until the data has been transmitted by
the instrument to the computer system.
During this time, the measuring time ("Time" on the display) continues to be counted. It is therefore
possible to achieve measuring times with values greater than two hours (the sedimentation values will
have been determined at the correct time, however).
The measurement is considered aborted if the [DEL] key is pressed after premature removal of the
Sedivette® (as suggested on the instrument's display). The data is then no longer available.
The measuring position is then vacant for a new measurement.
- A warning note is always given on the display if a Sedivette® is removed prior to the completion of
the measurement- New measurements cannot be started until the error has been rectified- If required, abort the measurement
Press the [DEL] key for this purpose. All data in the measuring record (identification number andmeasured data already determined) will be erased. The position can now be re-allocated.
- Measurement will be continued if the Sedivette® is re-inserted into the same position
The instrument treats and documents the error differingly, depending on the time at which the
Sedivette was removed, and how long it remained removeda) No measurement was being performed in the period for which the Sedivette® was removed- Measurement is continued- Both measured values can be determined at the correct timeb) The time for a measurement occurred during the period in which the Sedivette® remained
removed. Not more than five minutes elapsed between the time at which the measurement
should have occurred and the time at which the Sedivette® was re-inserted.
- The measurement which has been missed will be performed retrospectively 25 seconds after
re-insertion of the Sedivette®
- The measuring delay is less than five minutes and is not taken into account in any other way or
documentedc) The time for a measurement occurred during the period in which the Sedivette® remained
removed. More than five minutes elapsed between the time at which the measurement
should have occurred and the time at which the Sedivette® was re-inserted.
- The measurement which has been missed will be performed retrospectively 25 seconds after
re-insertion of the Sedivette®
- The measuring delay was greater than five minutes and is documented as follows:
Indication on display of incorrectly timed measurement in the list of ongoing measurements:- The measurement is performed retrospectively 25 seconds after the re-insertion of the Sedivette®
- As early as this point, the instrument detects incorrectly timed measurement of BSR- The instrument will display the list of ongoing measurements when the [DISPLAY] key is pressed. In
this case, a white "P" is displayed on a dark background after the affected sedimentation values(and before the "mm" data).

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Operating Manual Sediplus® S 2000, V 1.2
M.Pos.
1
1
1

ID number
10 0000000015
16 0000000010
17 0000000011

1h value
21Pmm---

2h value
30mm
20Pmm
--

Time
02:05
01:30
00:01

Display if a Sedivette® has been removed after measurement:- If a Sedivette® is removed after completion of measurement, the sedimentation values for thisSedivette® will appear immediately on the display. A "P" on a dark background is also seen in thisdisplay.
Important! This record remains visible for 15 seconds only. After this, the sedimentation values forthis position can be found in the "Values for Sedivettes removed" list.
-> SEDIMENTATION VALUES: Module 1 / Position 10
ID: 0000000015
1h value:
21Pmm
2h value: 30mm
Sedimentation time: 2 hours, 5 minutes"Values for Sedivettes removed" list:- The measured data for the Sedivettes removed can be viewed at a later time.
This is possible only as long as no new Sedivette® is inserted in the relevant position.
- The "Values for Sedivettes removed" list can be selected using the [DISPLAY] key- Leaf through the values for the individual positions in this list using the arrow keys
- The numerical keys can be used to switch to the record lists of other modules (if other modules havebeen installed)- A light-coloured "P" in a dark box is also apparent after the sedimentation value (and before the"mm" unit) in this display.
*** Values for SEDIVETTES removed ***-> SEDIMENTATION VALUES: Module 1 / Position 10
ID: 0000000015
1h value:
21Pmm
2h value: 30mm

Please note:
The Sedivette® can be removed until a measurement is due without the instrument
aborting the measurement. This means that removal of the Sedivette® after the zeromeasurement (for example) and its re-insertion into its position shortly before the
1 hour measurement will result in the instrument performing the overdue measurement
retrospectively. The same applies to removal after the 1 hour measurement timely
return to the position before the 2 hour measurement. The instrument performs ameasurement retrospectively as soon as the relevant Sedivette(s)® has been returned
to its position. The fact that measurement was not performed at the correct time isrecorded on the measuring record (as from five minutes of exceeded measuring time)
but not the duration of the delay, however.
Since sedimentation continues to progress, measurement at a delayed point will resultin an excessively high sedimentation value. The user must decide whether a delay istolerable for diagnosis purposes. Responsible procedure is that of discarding thesedimentation values in case of "late measurement" indicated by the Sediplus® S 2000
and performance of a new sedimentation measurement using a new blood specimen.
Please note that even slight vibrations of the Sedivette during removal of thespecimen between measurements are capable of causing incorrect results.

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Operating Manual Sediplus® S 2000, V 1.2

5.5 Power failures
No error is registered if the instrument is disconnected from the mains supply during a measurement
provided no measured-value registration cycle occurs during the period of disconnection from the
supply.
The built-in battery ensures that the data already determined remains preserved (stored). This is also
the case if the instrument is switched off after completion of all measurements and access to the data
is required only on the following day.
The capacity of the built-in battery is not adequate for performance of a measurement if ameasured-value registration cycle falls within the period of power failure, however.
The instrument performs the overdue measurement(s) retrospectively once the power supply has berestored.
A white "P" on a dark background is positioned between the data for the overdue mm value and the"mm" unit if the time of measurement has been exceeded by more than 5 minutes.
Please see the descriptive problems contained in Section 5.4, "Premature removal of a Sedivette®".
Here, the measurement cannot be performed, because the Sedivette® has been removed. The
instrument treats both of these incorrect operating actions (errors) identically.
Example of failure of power supply:- A measurement has been started. The instrument is disconnectedfrom the mains or
switched off a few minutes later.
- The interruption to power supply is noticed after (for example) 2 h and 10 min. andthe instrument is switched on again.
- After approx. 20 and 40 seconds, respectively, the instrument retrospectively
performs the two missing measurements for the 1 h and 2 h values.
->SEDIMENTATION VALUES: Module 1 / Position 10
ID: 0000000015
1h VALUE: 21Pmm
2h VALUE: 21Pmm
Sedimentation time: 2 hours, 30 minutes

The list of results is outputted on the display as shown above. Identical sedimentation
rates have been determined for the 1h and 2h values and displayed as "Pmm".
The same display also occurs if a Sedivette® is removed a few minutes after the startof measurement and re-inserted after the 130th. minute. Both measured values
feature a "P" on the record.

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Operating Manual Sediplus® S 2000, V 1.2

6 Cleaning the instrument
Please adhere under all circumstances to the specifications for cleaning of the
instrument. Inadequate cleaning and failure to adhere to the specifications may
result in malfunctions!
• Disconnect the instrument from the power supply for the purpose of cleaning• Exception: The Options menu contains the "Raise instrument plate for cleaning"menu item. This function is used to move the instrument plate upward. It remains
up for 120 seconds. The housing elements located underneath the instrument plate
can be cleaned during this time. The seconds remaining are indicated on thedisplay. This function can be aborted prematurely using the [ESC] key. In this case,the instrument plate is moved down again immediately.
• Do not spray cleaning agents under the instrument plate• Use only a slightly moistened cloth to clean the housing elements underneath the
instrument plate• Avoid under all circumstances contact with the PCB on the underside of the
instrument plate, contact with the connecting lead and contact with the guide
spindles• Operate the instrument at a voltage of 230 V. No liquid may penetrate into the
instrument during cleaning work.
• Always clean the instrument from outside only!
• Clean all accessible housing elements using a slightly moistened cloth. Soap
solution may also be used for this purpose. Then carefully dry the instrument.
• Never use scouring agents, aggressive cleaning agents or solvents• No disinfectant may penetrate into the interior of the instrument.
Never use disinfectants in spray canisters.
• Traces of dust or lint can be removed using compressed air• Never open the instrument in order to remove fouling within the housing• Have the instrument serviced by the DESAGA service department at regularintervals (please see Section 7, "Service and maintenance".
• The instrument must not be autoclaved
Blood is a potentially infectious substance; the relevant health and safety
regulations and conditions must therefore be adhered to under all circumstances
and all equipment cleaned and disinfected correctly.
Obtain within your Hygiene Department the necessary information concerning thedisinfection provisions necessary for your instrument (the instrument can, for example, be
sterilized in an ethylene-oxide chamber or cleaned using suitable disinfectants).
Example of a disinfectant:
The following aqueous solution can be used as a disinfectant:
25 g ethanol 96 %, 35 g 1-propanol, 0.1 g glyoxal to 100 g aqua dest. Allow this aqueous
mixture a number of minutes to act (depending on degree of contamination) and apply thedisinfectant solution again if necessary.
Then clean the instrument using a cloth slightly moistened with water and soap. Then dry
the instrument carefully.
Also recommendable are Sagrotan med. (disinfectant solution from Reckitt & Colman
Sagrotan GmbH) and Bacillol AF from BODE (for medical equipment in the sense of the
MPG).

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Operating Manual Sediplus® S 2000, V 1.2

7 Service and maintenance
Please contact the DESAGA Service department in Wiesloch or your local SARSTEDT sales agency
if you have any questions concerning or problems with the instrument. Never attempt to open or
repair the instrument yourself.
Important! In the majority of service cases, we require the Service Number and EPROM program
version for your instrument. The service number can be found on the model plate of your instrument.
The EPROM program version is shown on the display immediately after switching-on of the
instrument (see Section 4.1, "Switching on the instrument").
Defective instruments are inspected and repaired by our Service Center as rapidly as possible within
the framework of our Post-Based Repair Service system.
For this purpose, please return the defective instrument together with a description of the problem andthe completed contamination questionnaire and a copy of the delivery note, in the original packaging,to the following address or to your local SARSTEDT sales agency:
DESAGA GmbH / SARSTEDT GROUP
Service Department
In den Ziegelwiesen 1-7
Tel.:+49 (0) 62 22 / 92 88 65
Telefax: +49 (0) 62 22 / 92 88 60

D-69168 Wiesloch
Germany

Recommendation:
Have your instrument serviced by the manufacturer once each year.
Use SARSTEDT test Sedivettes to check your instrument for correct functioning.
Have your instrument inspected by the DESAGA GmbH Service Center in case ofdeviations from the defined sedimentation values.
(Test Sedivettes for Sediplus® S 2000: 1 set, SARSTEDT No.: 91.189 715)

8 Decommissioning and disposal
Important notes for disposal:
Since the Sediplus® S 2000 has been in contact with pathological material in yourlaboratory, please contact an approved specialist company or your local
hazardous-waste disposal company to ensure correct disposal of the instrument andavoid environmental harm.
SARSTEDT S-Sedivettes® and blood specimens used in conjunction with the
Sediplus® S 2000 instrument are potential sources of infection. Please always ensure
the necessary disinfection and correct disposal!
The spine and cover of this operating manual consist of PVC. The remainder consistsof paper and can be recycled after removal of the spine and covers.

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Operating Manual Sediplus® S 2000, V 1.2

9 Ordering information
Instrument/Accessories
Sediplus® S 2000, 230 V
Sediplus® S 2000, expansion module
Barcode reader for Sediplus® S 2000, 230 V
Thermo-printer with power pack (230/12 V)
Roll of thermo-paper for printer, 5 items
Sarmix® M 200, 230 V, inc. power pack
S-Sedivette®
Test Sedivettes for Sediplus® S 2000, 1 set

Order No.:
90.189 700
90.189 710
90.189 730
90.189 720
90.188 055
90.180 700
06.1690.001
91.189 716

Other blood sedimentation analyzers available from SARSTEDT AG & Co.:
Article:SEDIPLUS® S 200, 230 V
SEDIPLUS® S 200, 230 V, with computer/printer interface
SEDIPLUS® S 100, 230 V
SEDIPLUS® S 100, 230 V, with computer/printer interface
BSR Monovette® with membrane seal, with print
BSR Monovette® with membrane seal, with label
Plastic sedimentation pipette with installed plunger sleeve and safety stopper
Glass sedimentation pipette (0 marking)
Plunger sleeve for glass sedimentation pipette

Order No.:
90.189 600
90.189 604
90.189 500
90.189 504
05.10 7905.1079.001
86.19 9695.10 10
14.10 57

Please contact SARSTEDT at the following address if you have questions concerning other
SARSTEDT products or the SARSTEDT product range:
SARSTEDT AG & Co.
Postfach 1220
D-51582 Nümbrecht
Telefon:Telefax:+49 (0) 22 93-30 50+49 (0) 22 93-305-122

oder mit Ihrer lokalen SARSTEDT Verkaufsagentur in Verbindung.

10 Guarantee and liability
Our "General Terms and Conditions of Supply and Payment" apply in all cases. These
conditions can be found on the reverse of the invoice.
Any and all guarantee and liability claims shall be deemed excluded if they are the resultof one or more of the following causes:▪ Improper or incorrect use of the instrument▪ Incorrect installation, commissioning, start-up, operation or maintenance of the
instrument▪ Operation of the instrument with defective safety systems/equipment and/or withincorrectly fixed or non-functional safety and protective systems and equipment▪ Failure to adhere to the notes and remarks contained in the operating manual
concerning transportation, storage, installation, start-up and commissioning,
operation, maintenance and servicing, equipping and disposal▪ Unauthorized modifications to the instrument▪ Disasters resulting from the action of foreign bodies (??????; im deutschen Text steht"Fremdkörpereinwirkung"; Vorschlag: third parties) and/or Force Majeure▪ Incorrectly performed repairs
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Operating Manual Sediplus® S 2000, V 1.2

11 Glossary
Anticoagulant
BarcodeBarcode reader
Blood corpuscle sedimentation
rate (BSR)
Documentation
Expansion module
Basic module
Handshake
Identification number
Instrument plate
Measuring record
Measuring points
Room temperature
RS 232 interface
Sedimentation rate®
S-Sedivette
Westergren

Sodium citrate solution (0.106 mol/l), 0.7 ml pre-metered®
Patient identification, number on Sedivette or patient's record sheet
Scanner for reading of identification numbers
Erythrocyte sedimentation rate in x mm/h
Analysis results complete with patient's name and temperature
Expansion of basic module, up to three modules, each with 40 measuring
places40 measuring places
Communication
Instrument-specific distribution of analysis number, number entered at will, or
patient barcode
Guide plate for 40 Sedivettes with built-in sensor system and monitoring LEDs
Results bearing 1/2h values (½/1h values), date, time and identification
number;output via LCD display, serial printer or via interface to computer system®
40 specimen mounting for S-Sedivette
Temperature range (18 to 23° C as per DIN 58935, Part 1 [1], 18 to 25° C as
per NCCLS H2 to A3 [2], 20 ± 3° C as per BS 2554 [3] or as per national orlaboratory-specific definition)
Serial connecting instrument for data interchange
Rate of descent of erythrocytes in x mm/h
Safety blood-sampling system from SARSTEDT
Sedimentation value in mm/h

12 Index of illustrations
Abbildung 1 Rear view of the basic module (right) and two expansion modules ............................... 11
Abbildung 2: The rear panel of the Sediplus® S 2000....................................................................... 12
Abbildung 3: Connection of a keyboard and the optional barcode reader to the Sediplus® S 2000 .. 13
Abbildung 4 Front view of the Sediplus® S 2000 .............................................................................. 22

13 References
1) Deutsche Norm, Hämatologie, DIN 58935-1,
Bestimmung der Erythrozyten-Sedimentationsgeschwindigkeit im Blut
Teil 1: Ausgewählte Methode, Dezember 1997.
2) Methods for the Erythrocyte Sedimentation Rate (ESR) Test – Third Edition; Approved Standard. NCCLS
Document H2-A3, August 1993.
3) British Standard Specification for Westergren tubes and support for the measurement of erythrocyte
sedimentation rate, BS 2554:1987.

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Operating Manual Sediplus® S 2000, V 1.2

14 Index
Acoustic assistance 36
Anticoagulant 47
barcode reader 14
blood level 22, 38
blood sedimentation 6
Blood sedimentation 8cleaning 44
Communication 47
contamination 44
disruptive factors 28
Electromagnetic Compatibility Directive 24
exceeded measuring time 42
installation 12
instrument plate 21, 25
maintenance 5, 45
measuring record 17

Notes on Safety 6
options 17
peripherals 14
pin assignments 24
plasma 12
Power failure 43
Premature removal 41
RS 232 9
safety regulations 6
serial printer 8, 14
specimen 42
Symbols 5
system administrator 18
transportation damage 11
Underfilling 17, 22Westergren 9, 17, 35, 47

15 Contamination Questionnaire
Please complete the Contamination Questionnaire (Page 45) before returning the Sediplus® S 2000
instrument to DESAGA GmbH / SARSTEDT GROUP.
Please send the instrument, together with the completed Contamination Questionnaire, a short
description of the problem and a copy of the delivery note to:
DESAGA GmbH Service Department / SARSTEDT-GROUP Wiesloch Service Department or to yourlocal SARSTEDT sales agency.

Page 44Operating Manual Sediplus® S 2000, V 1.2

Contamination Questionnaire
for repair services
Dear Customer,
For safety reasons, we kindly request you to answer the following questions before returning
your instrument to DESAGA GmbH / SARSTEDT GROUP Wiesloch for maintenance, repair orsurrender.
Company:

Department:

Town:

Street address:

Name:

Telephone No.:

Instrument/Article:

SN:

We hereby confirm that the instrument is not contaminated.
The instrument is free of pollutants and other harmful substances and materials

The instrument has been in contact with the following pollutants and harmful
substances:
Substance category

Substance

Toxic substances
Corrosive substances
Explosive substancesRadioactive substance
Infectious substances
Inflammatory substances
Other hazardous substances
The instrument has been decontaminated in accordance with legal requirements(

Description of decontamination performed:

Date:

Signature:

Page 45

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Operating Manual Sediplus® S 2000, V 1.2

16 Annex
16.1 Initialization of the optional barcode reader

BarcodeThe optional SARSTEDT barcode reader is preprogrammed and initialized for thefollowing codes:- Code 39- UPC-A/UPC-E- 2/5 interleaved- Codabar- MSI- Code 128- Code 11- Code 39 Full ASCII- EAN-8/EAN-11
The reason for a barcode reader not detecting the barcode viewed,reading it
incorrectly or adding characters (e.g. preceding zeros) is often that a different codeis used for the barcode (e.g. transmission of check sums, etc.).
It is often helpful to initialize the barcode reader again.

For this purpose, use the Programming Chart for the barcode reader, which can be found on thefollowing page. This is valid only for optional barcode readers supplied by SARSTEDT!
Press the button on the left of the handle to initialize each barcode.
- Connect the barcode reader to the Sediplus® S 2000
- Switch the S 2000 and the barcode reader on- Perform initialization by moving the barcode reader downward across all codes on the Programming
Chart on the following page. The barcode reader emits an acoustic signal for acknowledgement ofeach barcode recognized.
Master Default- If this does not solve your problems, the barcode probably has a different code (e.g.: Barcode withcheck sum, etc.)- Please note the operating manual for the barcode reader

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Operating Manual Sediplus® S 2000, V 1.2

Barcodereader
P/N: 3820

SARSTEDT Default
FUZZY-LITE
ProgramOperation Mode

#5

Buzzer Tone Adjust#0

Preamble

#0

#6

#0

#D

FinishExit

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Operating Manual Sediplus® S 2000, V 1.2

16.2 Serial connecting cables
Sediplus® S 2000 / SARSTEDT log printer:Sediplus S 2000

SARSTEDT

9-pole socket

log printerDPU 201 GS
1
6

6

DSR

2
7

7
3

8

RxD

TxD

3
8

CTS

4

Busy
9

9
5

GND

5
GND

Sediplus® S 2000 / PC or EDV:
In cases in which the computer system/PC operates using the DTR (Data Transmission Ready)signal:PC or computer system serial interface

Sediplus S 2000

1

1

DSR

6

6
2

RXD

2

7

7
3

3

8

TXD

8
4

DTR

5

GND

4

9

9
5
GND

In cases in which the computer system/PC operates using the RTS (Request To Send) signal:

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