Primer Support 5G
OLIGONUCLEOTIDE PRODUCTION

Primer Support™ 5G is the fifth generation solid support for
oligonucleotide synthesis from Cytiva. This high-loaded solid
support is optimized for synthesis of DNA and RNA oligonucleotides
with capability up to 70 bases. The base particle is composed ofcross-linked polystyrene and delivers higher crude purity andsynthesis yield compared to previous generations of our solid
supports. These benefits are most evident for synthesis of RNA
oligonucleotides. This support is available derivatized with DNA
nucleosides, UnyLinker™, or RNA nucleosides. Primer Support 5G
has been proven in scalable processes from research toindustrial scale1.
Key benefits:•••

High loading capacity: A nucleoside loading of 350 µmol/g
for DNA and 300 µmol/g for RNA can be used withoutcompromising yield or purity. Suitable for synthesis of DNA andRNA oligonucleotides up to 70 bases. See the Synthesis oflong oligonucleotides section.

Table 1. Primer Support 5G characteristics

Matrixporous and polydispersed
divinylbenzene cross-linked
polystyrene particles

Cost efficient synthesis: With higher nucleoside loading,Primer Support 5G allows for major cost savings comparedto our previous generations of solid supports

Particle size
Swelling in acetonitrile

3.5 mL/g

Convenience and flexibility: UnyLinker can be used for any
DNA starting base with any modifications that are compatible
with the cleavage conditions (e.g., DNA or 2’OMe), hence
enabling stocking of one support

Swelling in toluene

7.5 mL/g

Storage

4°C to 30°C

Degree of nucleoside substitution

up to 400 μmol/g

Max recommended bed height

10 cm

Product characteristics
Primer Support 5G is derivatized with DNA nucleosides or UnyLinker
at 350 μmol/g and RNA nucleosides at 300 μmol/g. The base matrix
is made of 35–75 µm cross-linked polystyrene particles. The main
product characteristics are summarized in Table 1.

Scalability
Primer Support 5G provides excellent scalability with provenconsistency in purity and yield up to 1000 mmol synthesis scale1.
1

Fig 1. Primer Support 5G is a high-loaded solid support designed foroligonucleotide synthesis using phosphoramidite chemistry.

Data generated by Ionis Pharmaceuticals

cytiva.com

Recommended column packing volume2

38–75 µm (400–200 mesh)2

111 mg/mL (9 mL/g)

This recommendation is for a 20-mer. The recommended packing density varies with the sequencelength; see Packing density of Primer Support 5G section for details.

Synthesis of DNA oligonucleotides
Loading optimization
Primer Support 5G allows high nucleoside loading with maintained
performance in terms of yield, purity, N-1, and N+. Synthesis runs
were performed on ÄKTA™ oligopilot™ plus 100 using Primer
Support 5G with nucleoside loadings of 350 and 400 µmol/g.
A 20-mer phosphodiester DNA oligonucleotide (5’ ATA CCG ATTAAG CGA AGT TT 3’) was synthesized and the synthesis parameters
used are listed in Table 2 and summarized in Figure 2. The analytical
data are based on ion exchange (IEX)-HPLC analysis.

Table 2. DNA oligonucleotide synthesis parameters

Coupling optimization

Amidite concentration

0.1–0.2 M in acetonitrile (ACN)

Amidite excess

1.5–2.0 equiv.

Coupling

3.0 min recirculation1

Activator

0.3 M BTT

Oxidation

2.0 equiv. 50 mM I2 Pyr/H2O 9:1 (v/v)2

Ox CT

4.0 min3

Synthesis runs were carried out, using the preferred nucleosideloading of 350 µmol/g, to investigate the effect of different amountsof nucleotide monomer excess in the coupling reaction. Apart fromthe amidite excess, the same synthesis parameters were used as in
the previous experiment (see Table 2). The results are shown in
Figure 3.

Capping

0.5 Column volumes (CV), 0.5 min CT

Solid support

111 mg/mL CV

Column

6.3 mL

Oligonucleotide length

20

2
3

Yield (A260/µmol)

1

Recommendation: 2.0–3.0 min
Recommendation: 2.5 equiv.
Recommendation: 1.0 min (up to 4.0 min has not shown a negative effect)(A)

Yield vs amidite equiv. 350 µmol/g, test 20 DNA sequence
140

Purity vs loading for test 20 DNA sequence

130
120
110
100

100

1.4

1.5

1.6

85

1.9

2.0

2.1

80
75
70
280

300

(B)

320

340
360
Loading (µmol/g)

380

400

420

Yield vs loading for test 20 DNA sequence
150

Yield (A260/µmol)

1.8

Fig 3. Synthesis of a 20-mer DNA oligonucleotide using differing amountsof monomer excess in the coupling reaction. Yield is improved up to 1.75 equiv. ofamidite, without affecting purity, N-1, or N+. The plot shows yield vs amidite equiv.

90

140

The data demonstrate that between 1.5 and 2.0 equiv. of amidite,yield is improved up to 1.75 equiv. Full length purity was constantly~85% within this range of amidite excess. Based on the data,
preferred amidite excess is 1.75 equiv. for synthesis of DNA
oligonucleotides. Figure 4 shows an IEX-HPLC chromatogram after
synthesis of the 20-mer DNA oligonucleotide with a loading of
350 µmol/g and an excess of 1.75 equiv. of amidite during thecoupling step.
100

130

Purity: 87%
Yield: 132 A260/µmol

120
110
280

80
300

320

340
360
Loading (µmol/g)

380

400

420

Fig 2. Synthesis of a 20-mer DNA oligonucleotide gives high yield and purity athigh nucleoside loadings. Above 350 µmol/g a tendency of lowered purity was
repeatedly observed. Plots showing (A) Purity vs loading and (B) Yield vs loading.

The results show that the synthesis performance ofPrimer Support 5G is maintained for nucleoside loadings up to
350 µmol/g with a consistent yield of 125 to 135 A 260/µmol andpurity of 80% to 85%. The N-1 levels using Primer Support 5G,
loaded at 350 µmol/g, were consistently below 2%. Based on thegenerated data, the preferred nucleoside loading is 350 µmol/g forsynthesis of DNA oligonucleotides up to 25 bases.

Norm.

Purity (% FLP)

95

2

1.7

Amidites excess (equiv.)

60

40

20

N-1: 1.5%

0
0

10

20
Time (min)30

40

Fig 4. Chromatogram from IEX-HPLC analysis after synthesis of the 20-mer DNA
oligonucleotide using Primer Support 5G with a loading of 350 μmol/g and an
excess of 1.75 equiv. of amidite during the coupling step. Synthesis was
performed in a 6.3 mL column.

Primer Support 5G UnyLinker

75

A260 (mAU)

Primer Support 5G UnyLinker is a flexible alternative to standard
nucleoside-derivatized solid-phase supports, because it can beused as a starting position for any first base in the sequence.
Primer Support 5G UnyLinker is compatible with DNA, 2’OMe bases,
and other amidites with modifications that can survive at least nine
hours in ammonium hydroxide at 55°C, conditions required tocleave finished oligonucleotides from the support. Because ofthis requirement, UnyLinker is not suitable for use with
tert-butyldimethylsilyl (TBDMS) RNA amidites.

50

25

0
15

Coupling conditions

20

25

30
35
Time (min)

40

45

50

Synthesis runs were performed on ÄKTA oligopilot plus 10 running
UNICORN™ 5.31 software. Primer Support 5G was used with thepreferred UnyLinker loading of 350 μmol/g. Four batches were
evaluated. A standard UNICORN method was modified to extend
detritylation by 2–3 min in the first cycle, double the coupling timein the first cycle, and double the capping time in the first cycle.
Cleavage from the support was performed using ammoniumhydroxide for 14–16 h at 55°C according to the amidite supplier’s
instructions. A 21-mer phosphodiester DNA oligonucleotide wassynthesized using the parameters summarized in Table 3. The
results are shown in Figure 5.

Fig 5. Chromatogram from IEX-HPLC analysis after synthesis of the 21-mer DNA
oligonucleotide using Primer Support 5G with a UnyLinker loading of 350 µmol/g.
Four batches of UnyLinker support were evaluated using the same synthesis
parameters. Synthesis was performed on a 1.2 mL column.

Table 3. DNA oligonucleotide synthesis parameters

3. Double Capping in the first cycle

Amidite concentration

0.1 M in acetonitrile (ACN)* Method templates for ÄKTA oligopilot plus 10 and 100 are available on cytiva.com/oligo under the
documentation for the systems. Alternatively, search for product number 18114042 or 18113679.

Amidite excess

2.0 equiv.

Coupling

3.0 min recirculation

Activator

0.3 M BTT

Oxidation

4.0 equiv. 50 mM I2 Pyr/H20 9:1 (v/v)

Ox CT

1.0 min

Capping

0.5 CV, 0.5 min CT

Solid support

111 mg/mL CV

Column

1.2 mL

Oligonucleotide length21

Use of UNICORN software with UnyLinker
When using the UnyLinker support with Cytiva’s oligonucleotide
synthesizers, we recommend one or more of the following
modifications to the standard UNICORN methods*:
1. Extend Detritylation by 2–3 minutes in the first cycle
2. Double Coupling in the first cycle

Figure 6 shows an example of a modified UNICORN method.

The data in Figure 5 show the reproducibility of synthesis using
the UnyLinker support.
Fig 6. Standard UNICORN method with a coupling block and a capping block
inserted. The Detritylation flow is stopped at 3.00 min instead of 0.5 min, so an
extra 2:30 min Detritylation is added.

When using ÄKTA oligopilot plus 100 with Primer Support 5G
UnyLinker, a strategy upgrade is required to handle the high loaded
support. The strategy can be downloaded from our Life Sciences
Web site by searching for code number 29135879. When usingÄKTA oligopilot plus 10, a UNICORN strategy upgrade is notnecessary.

3

Synthesis of RNA oligonucleotides

Coupling optimization

Loading optimization

Using the preferred nucleoside loading of 300 µmol/g, synthesis toinvestigate the effect of using different amounts of monomer
excess in the coupling reaction were carried out. Apart from theamidite excess, the same synthesis parameters were used as in
Table 4. The results of these evaluations are summarized in Figure 8.
(A)

Purity vs amidite equiv., 300 µmol/g, 21-mer RNA sequence
90

Purity (% FLP)

To maximize the nucleoside loading for synthesis of RNA
oligonucleotides while maintaining performance in terms ofyield and purity, a set of synthesis runs were done using
Primer Support 5G with nucleoside loadings of 200, 300, and350 µmol/g. The synthesis parameters are listed in Table 4. The
sequence was a 21‑mer phosphodiester RNA oligonucleotide(5’ acg uug cag acu ccu aau gg T 3’). The results are summarized
in Figure 7. The analytical data are based on IEX-HPLC analysis.
Table 4. RNA oligonucleotide synthesis parameters

Amidite concentration

0.1–0.2 M in ACN

Amidite excess

1.5–3.5 equiv.

Coupling

12.0 min recirculation1

Activator

0.3 M BTT

Oxidation

4.0 equiv., 50 mM I2 Pyr/H2O 9:1 (v/v)2

Ox CT

1.0 min3

Capping

0.5 CV, 0.5 min CT

Solid support

111 mg/mL column volume

Column

6.3 mL

Oligonucleotide length21

Deprotection

20 mL Methylamine in ethanol(33% wt)/25% NH4OH (aq) 1:1,
55°C, 40 min
Evaporate
Add 5 mL DMSO
Add 5 mL TEA 3HF (TREAT),
60°C to 65°C, 3 to 3.5 h

80
70
60
50
1.0

(B)

1.5

2.0
2.5
3.0
Amidites excess (equiv.)

3.5

4.0

Yield vs amidite equiv., 300 µmol/g, 21-mer RNA sequence

Yield (A260/µmol)

150
130
110
90
1.0

1.5

2.0
2.5
3.0
Amidites excess (equiv.)

3.5

4.0

Fig 8. Synthesis of a 21-mer RNA oligonucleotide using differing amounts ofmonomer excess in the coupling reaction. Plots showing (A) Purity vs amidite
equiv. and (B) Yield vs amidite equiv. for replicate experiments.

Recommendation: 10.0–12.0 min
Recommendation: 2.5–4.0 equiv.
3
Recommendation: 1.0–4.0 min
1
2

As shown in Figure 7, the performance of Primer Support 5G ismaintained for nucleoside loadings up to 300 µmol/g. The full length
purity is consistently above 80% when the loading is up to
300 µmol/g. The yield is ~140 µmol/g even above this loading. The
N-1 levels using Primer Support 5G, loaded at 300 µmol/g, wereconsistently below 3%. Based on the generated data, the preferred
nucleoside loading is 300 µmol/g for synthesis of RNA
oligonucleotides up to 25 bases.

The generated data indicate that between 1.5 and 3.5 equiv. ofamidite, yield and purity is improved up to 2.0 equiv. Based on thedata, the preferred amidite excess is 2.0 equiv. for synthesis of RNA
oligonucleotides (Fig 9).
120

Purity: 81%
Yield: 149 A260/µmol

100
80
Norm.

Purity vs loading, 21-mer RNA sequence

Purity (% FLP)

90
85

40

80

20

N-1: 2.5%

0

75
70
180

5
200

220

240

260 280 300
Loading (µmol/g)

320

340

360

Fig 7. Synthesis of a 21-mer RNA oligonucleotide gives high yield and purity athigh nucleoside loadings up to 300 µmol/g. The plot shows purity vs. loading.

4

60

10

15
20
Time (min)25

30

Fig 9. Chromatogram from IEX-HPLC analysis after synthesis of the 21-mer RNA
oligonucleotide using Primer Support 5G with a loading of 300 μmol/g and an
excess of 2.0 equiv. of amidite during the coupling step. Synthesis was performed
in a 6.3 mL column.

(A)Packing density of Primer Support 5G

90
90

150 mg/mL

80
80
Norm.
Norm.

Optimization of packing densities
To evaluate the effect of packing density using
a nucleoside-derivatized support, we synthesized a model DNA
oligonucleotide 13-mer. Five packing densities between 22 and150 mg/mL CV were used. Oligonucleotides were synthesized onÄKTA oligopilot plus 10 running UNICORN 5.31 software. The
synthesis parameters are summarized in Table 5, and IEX-HPLC
data are shown in Table 6 and Fig 10.

00

Amidite excess

4.0 equiv.

Coupling

2.5 min recirculation

Activator

0.3 M BTT

Oxidation

4.0 equiv. 50 mM I2 Pyr/H20 9:1 (v/v)

Ox CT

1.0 min

Capping

2.0 CV, 0.5 min CT

Solid support

22–150 mg/mL CV

Column

1.2 mL

Oligonucleotide length

13

Norm.
Norm.
Norm.

(B)

0.1 M in acetonitrile (ACN)

40
40
20
20

Table 5. DNA oligonucleotide synthesis parameters

Amidite concentration

60
60

0
0

90
90
90
80
80
80
60

Norm.
Norm.
Norm.

(C)

20
Time20(min)Time (min)

30
30

40
40

10
10
10

20
Time20(min)Time20(min)Time (min)

30
30
30

40
40
40

142 mg/mL

60
60
40
40
40
20
20
20
0
00
0
0
0
50 mg/mL

90
90
90
80
80
80
60
60
60
40
40
40
20

20
20
0
00
10
20
30
40
0
Time20(min)
0
10
30
40
0
10
30
40
Time20(min)Fig 10. Chromatogram from IEX-HPLCTime
analysis(min)after synthesis of the 13-mer
DNA oligonucleotide
using Primer Support 5G with a loading of 300 µmol/g and90
a packing density of (A) 150, (B) 142, or (C) 50 mg/mL CV. Synthesis was performed
in a 1.280
mL column.

Table 6. DNA oligonucleotide test-13 synthesis using different packing densities

Solid support(mg/mL CV)

Scale(µmol)

Yield(AU260/µmol)

Total
AU260

150*

63.5

88

5600

142

†

60

92

5490

135

57

88

4950

50

21

89

1890

35

15

84

1246

22

10

73

675

Norm.

As shown by the data in Table 6 and Figure 10, Primer Support 5G
makes it possible to vary the packing density, and therefore thesynthesis scale, in the same column from 135 to 50 mg/mL CV withsimilar yield per µmol and similar purity. For synthesis of a 13-mer
we recommend a minimum packing density of 50 mg/mL, but wealso tested two packing densities below 50 mg/mL. In thisexperiment 35 mg/mL gave just slightly lower yield and purity
compared with 50 mg/mL (Table 6). Two runs with higher packing
density than the recommendation are included. They show that
there are high pressure alarms towards the end of the synthesis,but the results are still acceptable. Thus, it is possible to use slightly
higher packing density if the synthesis parameters are modified.

10
10

60
40
20
0
0

Purity(% FLP)

Purity(% N-1)

Ave. coupling
efficiency (%)

91

1.2

99.2

92

1.3

99.3

94

1.3

99.4

90
87

201.7
Time (min)
2.1

91

1.5

10

30

99.1

40

98.8
99.2

* high pressure alarm in last 5 cycles in oxidation plus last 3 cycles in detritylation†high pressure alarm in last 4 cycles, oxidation only

5The following analytical conditions were used for IEX-HPLC analysisof all synthesized oligonucleotides:
10 mM Tris, 10 mM NaClO4
10 mM Tris, 300 mM NaClO4
1%–70% in 40 min
2 µL
254 nmDionex NucleoPac PA100
35°C

(C)

The results* are summarized in Table 7 and Figure 11.
Table 7. Results of long DNA oligonucleotide syntheses

Length(bases)Packing density
Yield(mg/mL CV)(AU260/µmol)

FLP
(%)

Ave. coupling
efficiency (%)
98.9

30

95

198

73

40

83

255

61

98.7

50

72

303

57

98.8

60

67

362

44

98.6

70

62

337

36

98.6

Norm.
Norm.
Norm.
Norm.
Norm.

Buffer A:
Buffer A:
Gradient:
Injection:
Wavelength:
Column:Column temp:

(B)

Norm.
Norm.
Norm.
Norm.
Norm.

Primer Support 5G T was used at 350 µmol/g to synthesize
DNA oligonucleotides of five lengths: 30, 40, 50, 60, and 70
bases. The packing densities (Table 7) were adjusted based onrecommendations for oligonucleotide length. See the Packing
density for oligonucleotides > 30 bases section for more
information. For synthesis of these long oligonucleotides a large
excess of amidite (3.0-5.0 equiv.) and oxidation (4.0 equiv 50 mM I2
Pyr/H2O 9:1 [v/v]) was used to minimize the risk of coupling failure.
However, an amidite excess of 2.0 equiv., oxidation of 2.5 equiv.
50 mM I2 Pyr/H2O 9:1 (v/v), and an Ox CT of 2.0 min should be
sufficient for most syntheses.

Norm.
Norm.
Norm.
Norm.
Norm.

(A)

Synthesis of long oligonucleotides(D)

The results demonstrate that Primer Support 5G can be usedsuccessfully to synthesize oligonucleotides of at least 70 bases
when the packing density is adjusted for oligonucleotide length.

Norm.
Norm.
Norm.
Norm.
Norm.

FLP = full-length product* Note that results from analysis of oligonucleotides > 50 bases long are largely dependent on theinjected volume, gradient, column temperature, and integration parameters used to calculate thebaseline. Varying any of these parameters is likely to affect the results.

Norm.
Norm.
Norm.
Norm.
Norm.

(E)

90
90
90
90
80
90
80
80
80
60
80
60
60
60
40
60
40
40
40
20
40
20
20
20
0
200
0 0
0 0
0 0
90 0
90 0
90
90
80
90
80
80
80
60
80
60
60
60
40
60
40
40
40
20
40
20
20
20
0
200
0 0
0 0
0 0
90 0
90 0
90
90
80
90
80
80
80
60
80
60
60
60
40
60
40
40
40
20
40
20
20
20
0
200
0 0
0 0
0 0
90 0
90 0
90
90
80
90
80
80
80
60
80
60
60
60
40
60
40
40
40
20
40
20
20
20
0
200
0 0
0 0
0 0
90 00
90
90
90
80
90
80
80
80
60
80
60
60
60
40
60
40
40
40
20
40
20
20
20
200
0
0 0
0 0
0 0
0
0

30-mer1010
10
1040-mer10

20
30
20 Time (min) 30
20
30
(min)
20 TimeTime (min) 30
20 Time (min)30
Time (min)

40
40
40
40
40

50
50
50
50
50

10
10
10
10
10

20
30
20 Time (min)30
20 Time (min)30
20 Time (min)30
20 Time (min)30
Time (min)

40
40
40
40
40

50
50
50
50
50

10
10
10
10
10

20
30
20 Time (min)30
20
30
20 Time (min)30
20 Time (min)30
Time (min)Time (min)

40
40
40
40
40

50
50
50
50
50

20
30
20 Time (min)30
20
30
20 Time (min)30
20 Time (min)30
Time (min)Time (min)

40
40
40
40
40

50
50
50
50
50

20
30
20Time (min) 30
20
30
(min)
20TimeTime (min) 30
20 Time (min)30
Time (min)

40
40
40
40
40

50
50
50
50
50

50-mer60-mer

10
10
10
10
10

70-mer

10
10
10
10
10

Fig 11. Chromatogram from IEX-HPLC analysis after synthesis of 30-, 40-, 50-,
60-, and 70-mer DNA oligonucleotides using Primer Support 5G and the packing
densities listed in Table 7. Synthesis was performed in a 1.2 mL column.

6Packing density for oligonucleotides > 30 bases

150
A260 (mAU)

A modified packing density is an option that enables synthesis oflong sequences using the same stocked solid support. Decreasing
packing density as the sequence length increases helps to maintainpressure towards the end of the synthesis within acceptable levels.
The recommendations in Figure 12 apply to both nucleoside- andUnyLinker-derivatized supports. Note that when synthesizing DNA
or RNA oligonucleotides > 30 bases long, it is necessary to switchfrom 260 nm to a more sensitive UV1 wavelength to ensure efficient
integration and UV watch steps. Typically, 500 nm is used.

Primer Support 5G T, 350 µmol/g
Primer Support 40s, 41 µmol/g

200

100
50
0
10

160

20

25
30
Time (min)

35

40

45

Fig 13. Chromatogram from IEX-UHPLC analysis after synthesis of a 50-mer DNA
oligonucleotide using Primer Support 5G T, 350 µmol/g or Primer Support 40s,
41 µmol/g, Synthesis was performed in a 1.2 mL column.

140
Packing density (mg/mL)

15

120
100

Synthesis of 50-mer DNA oligonucleotides on both supports
generated similar IEX-UHPLC profiles. The percentage offull-length product was 52% for Primer Support 5G and 54%
for Primer Support 40s.

80
60
40
20
0
10

20

Oligonucleotide length

30
40
50
Oligonucleotide length

13

60

70

20

30

40

50

60

70

Packing density (mg/mL CV)* 135 111

95

83

72

67

62

* Note: To synthesize RNA oligonucleotides > 25 bases, reduce the packing density by 		
approximately 10%.

Fig 12. Packing density recommendations for Primer Support 5G based onoligonucleotide length.

Comparison of Primer Support 5G
and Primer Support 40s foroligonucleotides > 30 bases
Synthesis runs were performed on ÄKTA oligopilot plus 10 using
either Primer Support 5G T with nucleoside loading of 350 µmol/g
or Primer Support 40s with nucleoside loading of 41 µmol/g.
A 50-mer was synthesized using either support. Analysis was
performed using IEX-UHPLC.
IEX-HPLC analysis results are provided in Figure 13. UHPLC wasused instead of HPLC in order to improve the resolution between
full-length product and shorter products.

Total solution for production oftherapeutic oligonucleotides
For over 25 years, Cytiva has been providing high quality
solid supports for the process development and production ofoligonucleotides. In addition to providing reliable solid supports,
Cytiva has proven expertise in designing customized process-scale
instrumentation for the synthesis of oligonucleotides from 10 mmol
upwards. Our OligoProcess™ systems are custom-designed
through careful planning with each production facility. Our
well-established global distribution network allows material for yourproduction facilities to be obtained world wide.
ÄKTA oligopilot plus 10 and 100, and OligoPilot 400
instruments have a proven reputation in process development
and in validated manufacturing processes. Our experienced
personnel have comprehensive knowledge and expertise ofoligonucleotide production, regulatory aspects, and installation
qualification/operational qualification (IQ/OQ).

Regulatory support
Cytiva has decades of experience working with biopharmaceuticalmanufacturers in validating their production processes. This
expertise is also available for our oligonucleotide synthesis
instruments for process development and production. In addition,
we provide essential information for the registration of materials
used for oligonucleotide production in the form of confidential
Regulatory Support Files. For more detailed information, contact
Fast Trak Validation Services or contact your local Cytiva
representative.

7

Custom Primer Support

Products

Cytiva offers Custom Primer Support to meet your exact needs bycoupling with linkers, labels, modified bases, alternative protecting
groups, or almost any molecule of your choice. Primer Support 5G
can be loaded at 20 to 400 μmol/g and delivered in bulk or in
prepacked, disposable Oligosynt™ columns. For more information,please contact your local sales representative or go tocytiva.com/oligo.

Ordering information
Products

Product code

Primer Support 5G ribo A 300

1 mmol28996440

Primer Support 5G ribo A 300

10 mmol

28996444

Primer Support 5G ribo C 300

1 mmol28996441

Primer Support 5G ribo C 300

10 mmol

28996445

Primer Support 5G ribo G 300

1 mmol28996442

Primer Support 5G ribo G 300

10 mmol

28996446

Primer Support 5G ribo U 300

1 mmol28996443

Primer Support 5G ribo U 300

10 mmol

28996447

Primer Support 5G T 300

1 mmol29014619

Primer Support 5G T 300

10 mmol

29014620

Quantity

Product code

1 mmol28996425

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Primer Support 5G dA 350

10 mmol

28996430ÄKTA oligopilot plus 1018114042

Primer Support 5G dC 350

1 mmol28996426

ÄKTA oligopilot plus 100

18113679
28989965
on request

Primer Support 5G dC 350

10 mmol

28996431

OligoPilot 400

Primer Support 5G dG 350

1 mmol28996427

OligoProcess

Primer Support 5G dG 350

10 mmol

28996432

Related literature

Primer Support 5G T 350

1 mmol28996428ÄKTA oligopilot plus, Data file

Primer Support 5G T 350

10 mmol

28996433

Oligosynthesis product guide

18117275

Primer Support 5G UnyLinker 350

1 mmol29025962

Optimization and scaleup of siRNA synthesis

28405796

Primer Support 5G UnyLinker 350

10 mmol

29025963

cytiva.com/oligo
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco
LLC or an affiliate. ÄKTA, Primer Support, OligoPilot, OligoProcess, Oligosynt,
and UNICORN are trademarks of Global Life Sciences Solutions USA LLC or anaffiliate doing business as Cytiva.
UnyLinker is a trademark of Ionis Pharmaceuticals, Inc. All other third-party
trademarks are the property of their respective owners.
ÄKTA oligopilot and OligoPilot 400 is covered by US patent number
5,807,525 and equivalent patents and patent applications in other countries.
Post-synthesis procedures for selective deprotection of oligonucleotides
on solid supports is covered by US patent number 6,887,990 and equivalent
patents and patent applications in other countries, and a license thereto
is only given when synthesis of oligonucleotides is performed on Cytiva
instruments. No other license is granted to the purchaser either directly or
by implication, estoppel or otherwise.
© 2020 Cytiva
All goods and services are sold subject to the terms and conditions of saleof the supplying company operating within the Cytiva business. A copy of
those terms and conditions is available on request. Contact your local Cytiva
representative for the most current information.
For local office contact information, visit cytiva.com/contact

CY13694-24Aug20-DF

18114466