Evec, Inc.

www.evec.jp

Production of Human Monoclonal Antibody
Our company uses EBV-treated human PBMC (Peripheral Blood Mononuclear Cells) to clone andobtain fully-human monoclonal antibodies

We offer assistance for the commercialization of drug
discovery seeds and antibody development for drugcandidates
The benefits of our fully-human antibody production service• We screen the possibility to obtain a target
antibody from our company’s PBMC library thatoriginated from more than 100 healthy donors. Ifthe possibility to obtain the target antibody fromhealthy donor library is considerably high, wecan start the antibody screening from ourspecimens.
• For antibodies that are only expressed in specificdiseases or antibodies that are difficult to becloned from healthy donor specimens, it ispossible to screen the PBMC specimens from thecostumer. In such cases, we can start the DNA
cloning step from the specimens that we receive.
• By using our optimized technologies (flowcytometry or micro array), the antibody producer
cells can be concentrated or isolated such that it
is possible to obtain the antibody DNA of interest
even with a low number of the target antibody
producing cells. These techniques dramatically increase the possibility to clone the target
antibody DNA.
• Our company’s antibody DNA cloning service emphasizes on obtaining antibodies with originalactivity, thus we normally only construct full-length heavy and light chains, and do not construct
antibody derivatives such as scFv.

Flow Cytometry
Flow cytometry saves time and is an eﬃcient way to collect positive cells
without limiting dilution
If it is possible to prepare the antigen thatcan bind to living cells, flow cytometry can beused as a powerful method to concentrate
positive cells, as the B cells obtained from acandidate donor also express antibodies on theircell surface. By the introduction of this sorting
method, it is now possible to quickly separate
and recover the B-cells that produce the target
antibodies without the time-consuming limited
dilution method.
Production of Human Monoclonal Antibody

1Ver.3 160719

Evec, Inc.

www.evec.jp

Cell Micro Array
Strategic isolation of particular antibody-producing B-cell by cell microarraysystemEvec’s original cell micro array method was developed
to isolate positive cells that produce the antibody ofinterest. The method is performed by dropping anantibody-producing cell into each of 90,000 wells in a
micro array chip, followed by identification of positive
wells using a slide glass coated with the antigen.
As each positive well contain single antibody-producing
cell, it is possible to isolate each positive cell by using acapillary, followed by single-cell RT-PCR to isolate andsequence the antibody DNA fragments. Because it ispossible to clone the antibody DNA with only 1 positivewell, theoretically cloning can be performed although thepossibility of obtaining positive cell is only 0.01%.
Furthermore, as the cell micro array isolates antibody
DNA from single cells, the identification of positive genes
and the pairing of light and heavy chain can be performed
rapidly.

For more information, please contact:

Evec, Inc.
Antibody Drug Development Laboratory
1 Techno Park Shimonopporo, Atsubetsu-ku
Sapporo 004-0015, Japan

Email: support@evec.jp

Production of Human Monoclonal Antibody

Web: http://www.evec.jp/2Ver.3 160719