EPIGENTEK
Complete Solutions for Epigenetics

EpiMag™ HT (96-Well) Magnetic Separator
Base Catalog # Q10002
INTENDED USE
EpiMag™ HT (96-Well) Magnetic Separator allows paramagnetic bead precipitation of liquid samples from various
96-Well microplates. It can be used for isolation and purification of nucleic acids and proteins, immunoprecipitation,immunoassays (ELISA), cell sorting, and purification of biomolecules.

PRODUCT CONTENTS
Component
96-Well Magnetic Separator
96-Well Microplate
Adhesive Cover Film

Cat. #Q10002
111

Storage Upon ReceiptRTRTRTSHIPPING & STORAGE
The product is shipped at ambient temperature. Upon receipt: Store this magnet at room temperature and a safe
distance from magnetic storage devices such as hard disks and cards with magnetic stripes, such as credit cards.
Keep this magnet away from electronic devices and persons with cardiac pacemakers.

GENERAL PRODUCT INFORMATION
Quality Control: EpiMag™ HT (96-Well) Magnetic Separator is tested against predetermined specifications toensure consistent product quality. EpiGentek guarantees the performance of all products in the manner described inour product instructions.
Product Warranty: If this product does not meet your expectations, simply contact our technical support unit or yourregional distributor. We also encourage you to contact us if you have any suggestions about product performance or
new applications and techniques.
Safety: Suitable lab coat, disposable gloves, and proper eye protection are required when working with this product.
Product Updates: EpiGentek reserves the right to change or modify any product to enhance its performance anddesign. The information in this User Guide is subject to change at any time without notice. Thus, only use the User
Guide that was supplied with the product when using that product.
Usage Limitation: The EpiMag™ HT (96-Well) Magnetic Separator is for research use only and is not intended for
diagnostic or therapeutic application.
Intellectual Property: The EpiMag™ HT (96-Well) Magnetic Separator and methods of use contain proprietary
technologies by EpiGentek.

PRODUCT DESCRIPTION
The implementation of twenty-four extremely powerful magnetic rods made of neodymium-iron boron enables fastand easy magnetic separation. Each magnetic rod addresses four wells of the 96-well plate. With this magnetic
separator, paramagnetic beads will be firmly pulled to the side of the plate wells, which ensures complete removal ofthe magnetic beads from the solution by a robot or with a multichannel pipette, minimizing sample loss. This producthas the following features:
110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735
Tel: 1-877-374-4368 ■ Fax: 1-718-484-3956 ■ E-mail: info@epigentek.com ■ Web: www.epigentek.com© EpiGentek Group Inc. All rights reserved. Products are for research use only.

Page 1Printed 2022-01-20Q10002

EPIGENTEK
Complete Solutions for Epigenetics•

Ultra-Powerful NdFeB Magnets - allows for the fastest separation times and minimal bead loss.

•Efficient Particle Washing - Vertical orientation of the bar magnetic field attracts and suspends the magnetic
particles both to the side and up from the bottom of the wells. Pipette tips can be inserted to the bottom of thewell, insuring thorough and efficient washing and removal of fluids.

•Maximum Sample Concentration - Samples can be efficiently recovered from 10 µl to 2 ml of liquid.

•Universal Compatibility – Suitable for most magnetic beads or particles including magnetic iron oxide
nanoparticles and magnetic agarose beads (e.g., EpiNext beads, AMPure XP, Axygen, etc). Compatible with all
microtiter plates, including standard 96-well plates (0.25 to 0.3 ml wells) and deep well plates (1 to 2 ml) withvarious well bottoms (U-bottom, V-bottom, and flat bottom).

•Lightweight Handheld Design - Allows for easy repeated handling.

PROTOCOL OF USE
The working procedure is described as the follows:Standard or Deep-Well Microplate1.

Add the sample solution into a microplate (if the microplate does notcontain sample solution), then add appropriate binding buffer (ifnecessary) and magnetic beads into the plate for an appropriate
incubation time.

2.

Place the microplate onto the HT (96-Well) Magnetic Separator. The
magnetic beads will aggregate to the side or bottom of the well. The
time period of complete capture of the beads is dependent on thesolution volume and size of the beads, ranging from several seconds
to several minutes.

3.

Remove the fluid completely with a pipette and wash the beads withappropriate wash solution several times. Leave the plate on theseparator for 1-4 min to to ensure optimal removal of any residual fluid.

4.

Elute the sample molecules from the beads with appropriate elution
buffer.

PCR Microplates
Note: PCR microplates have narrow wells which increases surface
tension and makes removing the excess fluid of the original sample fromthe beads difficult. For the best results, we recommend transferring thesample solution in the PCR plate into the 96-well microplate included withthe separator or a standard microplate.

_________________________________________
Fig 1. Workflow for using EpiMag™ HT (96-well)
Magnetic Separator for sample molecule isolation.

RELATED PRODUCTS
Q10003
P-1059P-1060P-1063
P-1064

EpiMag™ 96-Well MicroplatePEpiNext™ DNA Size Selection Kit
EpiNext™ NGS Barcode (Index) Set-12
EpiNext™ DNA Purification HT System
EpiQuik™ Circulating Cell-Free DNA Isolation Kit

110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735
Tel: 1-877-374-4368 ■ Fax: 1-718-484-3956 ■ E-mail: info@epigentek.com ■ Web: www.epigentek.com© EpiGentek Group Inc. All rights reserved. Products are for research use only.

Page 2Printed 2022-01-20
Q10002