国立環境研究所研究報告 第182号
ISSN 1341-3643
Research Report from the National Institute for Environmental Studies, Japan No. 182, 2004

R-182-2004

NIES-Collection

LIST OF STRAINS
Seventh Edition
2004
Microalgae
andProtozoa

Edited by
Fumie Kasai, Masanobu Kawachi,
Mayumi Erata and Makoto M. Watanabe
Supervised byCommittee for Evaluating Microbial Culture Strains

National Institute for Environmental Studies
JAPANNIES-Collection. List of Strains
Seventh Edition
Microalgae and Protozoa
March 1, 2004

Contributors:
Fumie Kasai, Masanobu Kawachi,
Mayumi Erata, Makoto M. Watanabe,
Akira Shimizu, Fumi Mori, Kosei Yumoto,
Mayumi Moriya and Miwa Ishimoto

Acknowledgment
Sincere thanks are expressed to Dr. T. Nakayama, University of Tsukuba, and Ms. T.
Suzuki and S. Takasaki, National Institute for Environmental Studies, for their helpfuladvice and technical assistance about the taxonomy and the maintenance of some strains.

Cover design : Fumi Mori

−ii−

第七版の序国立環境研究所微生物系統保存施設が、2000 年に保存株リスト第六版を発行してから、４年を迎えることとなった。初版〜六版に関して、国内国外の各方面から多くの建設的意
見や激励が寄せられたことには非常に勇気づけられたと共に、我々の事業が環境科学分野
のみならず、基礎生物学、農学、水産学、食品学、医学等の分野でも注目され、重要視されていることを知り、責任の重さを痛感したものである。
この第七版は、初版〜六版と同様に微生物系統保存株評価委員会の監修を受け、微細藻類 1,215 株、原生動物 6 株を掲載した。特に保存株の分類、保存株特性については注意深
い検討がなされたが、不備な点はご指摘願えれば幸いである。
本施設に保存されている微生物株の殆どは、わが国の藻類学者によって分離培養されたものであり、他の微生物保存機関には保存されていないものである。今後、貴重な微生物株については、国内国外の微生物保存機関と密接な連携・協力関係を組み、共通のルールで共有していくことを考えている。また、本施設の事業は、微生物株の収集・保存・分譲にとどまらず、分類学的研究、保存技術の開発、株情報の収集およびその電算機管理シス
テムの開発等多岐に亘っているが、これらの事業が益々充実し成果をあげるために、施設・要員の充実と拡充をはかっていく所存である。今後とも一層のご批判とご支援を賜ることができれば幸いである。
最後に、寄託依頼された藻類株の評価並びに本リスト刊行に際して様々なご指導とご助言をいただいた評価委員会委員に深甚な謝意を表するとともに、微生物系統保存施設のスタッフ一同の熱意に満腔の敬意を表したい。

平成１６年３月

国立環境研究所微生物系統保存株評価委員会委員長
国立環境研究所生物圏環境領域長

渡

−iii−

邉

信

第六版の序国立環境研究所微生物系統保存施設が、1997 年に保存株リスト第五版を発行してから、３年を迎えることとなった。初版〜五版に関して、国内国外の各方面から多くの建設的意
見や激励が寄せられたことには非常に勇気づけられたと共に、我々の事業が環境科学分野
のみならず、基礎生物学、農学、水産学、食品学、医学等の分野でも注目され、重要視されていることを知り、責任の重さを痛感したものである。
この第六版は、初版〜五版と同様に微生物系統保存株評価委員会の監修を受け、微細藻類 752 株、原生動物 2 株を掲載した。特に保存株の分類、保存株特性については注意深い
検討がなされたが、不備な点はご指摘願えれば幸いである。
本施設に保存されている微生物株の殆どは、わが国の藻類学者によって分離培養されたものであり、他の微生物保存機関には保存されていないものである。今後、貴重な微生物株については、国内国外の微生物保存機関と密接な連携・協力関係を組み、共通のルールで共有していくことを考えている。また、本施設の事業は、微生物株の収集・保存・分譲にとどまらず、分類学的研究、保存技術の開発、株情報の収集およびその電算機管理シス
テムの開発等多岐に亘っているが、これらの事業が益々充実し成果をあげるために、施設・要員の充実と拡充をはかっていく所存である。今後とも一層のご批判とご支援を賜ることができれば幸いである。
最後に、寄託依頼された藻類株の評価並びに本リスト刊行に際して様々なご指導とご助言をいただいた評価委員会委員に深甚な謝意を表するとともに、微生物系統保存施設のスタッフ一同の熱意に満腔の敬意を表したい。

平成１２年３月

国立環境研究所微生物系統保存株評価委員会委員長
国立環境研究所生物圏環境部長

渡

−iv−

辺

信

第五版の序国立環境研究所微生物系統保存施設が、1994年に保存株リスト第四版を発行してから、3年を迎えることとなった。初版、二版、三版並びに四版に関して、国内国外の各方面から多くの建設的意見や激励が寄せられたことには非常に勇気づけられたと共に、我々の事業
が環境科学分野のみならず、基礎生物学、農学、水産学、食品学、医学等の分野でも注目され、重要視されていることを知り、責任の重さを痛感したものである。
この第五版は、初版、二版、三版並びに四版と同様に微生物系統保存株評価委員会の監修を受け、微細藻類619株、原生動物3株を掲載した。特に保存株の分類、保存株特性については注意深い検討がなされたが、不備な点はご指摘願えれば幸いである。
本施設に保存されている微生物株の殆どは、我が国の藻類学者によって分離培養されたものであり、他の微生物保存施設には保存されていないものである。今後、貴重な微生物株については、国内国外の微生物保存機関と密接な連携・協力関係を組み、共通のルールで共有していくことを考えている。また、本施設の業務は、微生物株の収集・保存・分譲にとどまらず、分類学的研究、保存技術の開発、株情報の収集および株情報の電算機管理システムの開発等多岐に亘っているが、これらの業務を益々充実させ成果をあげていく所存である。今後とも一層のご批判とご支援を賜わることができれば幸いである。

平成９年３月
国立環境研究所微生物系統保存株評価委員会委員長
国立環境研究所生物圏環境部長

岩 熊 敏 夫−v−

保存株リスト第一版発刊に寄せて
国立環境研究所に我が国最初の環境微生物の系統保存施設が設置されたのは、昭和58年1月であったが、その後約2年間にわたって、同研究所の関係者の並々ならぬ努力によって、微生物保存事業に関する周到なる準備作業が繰り展げられ、ようやくここにその成果を保存株リストとして集大成されたことは、環境科学にたずさわる多くの研究者にとって、これ程慶ばしいことはない。ここに関係者各位に対して満腔の敬意を表明したい。
今回刊行された保存株リストは、当面環境生物学上重要な生産者である微細藻類に的を絞ったものであるが、これは我が国の現行微生物系統保存事業のうちで最も弱点とされていた分野であり、学界・産業界からもその実現が強く要望されていたところである。微細藻類の系統保存は、長年にわたり活発に研究されてきた細菌類や菌類の系統保存とは異なり、その分離、培養、保存等の条件が極めて複雑で、技術的に多くの困難な作業を伴うものである。本研究所においてはその性格上多角的研究に取り組んでいるが、その特徴を生かして所内の衆知を結集してこの点を克服し、世界的に通用する信頼度の高い系統保存事
業を軌道に載せることに成功した。本施設の保存する微生物株は、その特性が科学的に実証されているために、これを実験的に使用する研究者、あるいはそれら微生物株データの利用者にとって、高い信頼感をもって利用することができる。しかも本施設では、保存微生物株に関する独自の電算機管理システムを開発したので、その保存株データを環境生物に関するデータベースの一環として利用することが可能となった。このことによって、とかく遅れがちであった我が国環境生物学の近代化が著しく促進されるものと信ずる。
本施設の当初の目標は環境問題に関係ある多種多様の微生物株を総合的に収集保存することにあったが、現状ではようやく微細藻類についての系統保存体制が確立されたに止まっている。今後益々施設設備の充実をはかって、微細藻類のみならず、環境生物学の調査研究上欠かすことのできないその他の微生物の系統保存をも実施し、名実ともにそなわった世界的な環境微生物株保存センターの一つとして発展されることを期待したい。
昭和60年2月

元富山大学長東京大学名誉教授

柳 田 友 道

−iv−

目

次

第七版の序.............................................................. 渡 邉信保存株リスト第一版発刊に寄せて .......................................... 柳 田 友 道
Ⅰ．はじめに ...................................................................... 1
Ⅱ．培養株の寄託 .................................................................. 4
１．寄託条件 ................................................................... 4２．寄託にあたっての同意事項 ................................................... 4
３．寄託方法 ................................................................... 5Ⅲ．保存株の分譲 .................................................................. 9
１．分譲にあたっての同意事項 ................................................... 9２．株の注文方法 .............................................................. 10３．株の価格 .................................................................. 10４．"Untransportable"（輸送が困難な）株と凍結保存株の分譲についての注意 .......... 10５．1993年以降採集された外国産株の取り扱い .................................... 11
６．有毒物質を生産する株の取り扱い ............................................ 11
７．国立環境研究所の職員、客員研究員、共同研究員への分譲 ...................... 11
Ⅳ．分譲株の培養保存法 ........................................................... 19
Ⅴ．藻類培地作成の基本手法 ....................................................... 19
１．保存試薬液 ................................................................ 19２．培地作成 .................................................................. 21
３．寒天斜面培地 .............................................................. 22Ⅵ．培地（Media）................................................................ 49
１．藻類の保存培地（Stock media for algae）....................................... 49
２．無菌検査培地（Bacteria-free check media） ..................................... 56
３．微量金属混液・ビタミン混液・土壌浸出液（Trace metals, vitamin mixes and soil extract）................................... 57
４．原生動物の保存培地（Stock medium for protozoa） .............................. 58
Ⅶ．保存株データの利用法 ......................................................... 59
Ⅷ．保存株データ（Strain data） .................................................... 63
付表１．凍結保存株一覧（List of cryopreserved strains） ......................... 188
付表２．有毒株一覧（List of toxic strains）..................................... 190
付表３．遺伝子データ一覧（List of gene data） ................................. 191
Ⅸ．索引（Indexes）............................................................. 198
１．株番号索引（Numerical index）............................................. 198
２．分類群別索引（Systematic index） .......................................... 211
Ⅹ．文献（References） .......................................................... 226−v−

I. はじめに独立行政法人国立環境研究所微生物系統保存施設（NIESコレクション）は1983年に環境微生物の系統保存を行うことを目的として設立された。当時は湖沼の富栄養化や大気汚染が現在より深刻な時代であった。そのような時代に、NIESコレクションは環境研（当時は公害研）内の研究者が分離した株や他のコレクションから移管された微細藻類株、250株余りでスタートを切った（文献526, 527）。小さなコレクションとしてのスタートであったが、まさに時を得ていたといえる。設立当時はシャットネラやヘテロシグマといった赤潮形成藻、ミクロキスティスのような水の華を形成するシアノバクテリアが代表的な保存株であり、これらは現在でも諸外国のコレクションにはないユニークなコレクションとしてのNIESコレクションの特徴となっている。
保存株
研究者からの寄託と保存施設における独自の分離によって、設立から20年の間に保存株
数は1,400株余りに増加し、コレクションは大きく躍進した (Fig. 1)。この保存株リスト
第７版は、このうち微細藻類1,215株と原生動物6株をNIES株として掲載している。微細藻類については現在知られているほとんどの綱を網羅している(Table 1)。
NIESコレクションでは、ほとんどの株が5〜25℃、4〜40μmol / m2sec (12時間ごとの明暗周期)の最適増殖条件あるいはそれに準ずる環境で維持され、10日〜4ヶ月ごとに新しい培地に植え継がれている（継代培養）。培養条件は、このリストの第Ⅷ章に示されているが、株によって異なる。また、継代培養中に何らかの原因で保存株の状態が悪くなり、最終的に株を失ってしまう危険性をコレクションは常にもっている。これをできる限り防ぐために保存株の生育状態を毎週チェックしている。また、現在保存されている株のうち400株程度が無菌培養株である。これらは遺伝学や生理学の研究には欠かせない。これらの無菌培養株については毎年無菌チェックを行っている。
デジタル画像や18Sリボソーム遺伝子の塩基配列データの収集も業務として行い、保存株の評価に用いている。これらのデータは、通常3年ごとに出版している保存株リスト（文献521, 528, 529, 531, 532, 540,541）における分類学的位置の確認
緑藻

保
存
株
数

などに利用され、またホームページ（URL: http://www.nies.go.jp/biology/

ラフィド藻
珪藻
渦鞭毛藻

mcc/home.htm）でも検索できるよう

ｼｱﾉﾊﾞｸﾃﾘｱ

Fig. 1. 第１版〜７版に掲載された保存株数

準備が進められている。

の推移。緑藻は狭義の緑藻綱、ペディノ藻
その他

綱、アオサ藻綱、トレボキシア藻綱、車軸藻綱を含む。

年-1-

Table 1. NIESコレクションに保存されている保存株の属、種、株数
門
藍色植物門
灰色植物門
紅色植物門
クリプト植物門
不等毛植物門

ハプト植物門
渦鞭毛植物門ユーグレナ植物門
クロララクニオン植物門
緑色植物門

原生動物

綱

属

種/変種

株

藍藻綱
灰色藻綱
紅藻綱
クリプト藻綱
黄金色藻綱
ラフィド藻綱
ディクチオカ藻綱
珪藻綱
褐藻綱
黄緑色藻綱
ペラゴ藻綱
ピングイオ藻綱
シゾクラディオ藻綱
プリムネシオ藻綱
パブロバ藻綱
渦鞭毛藻綱
ユーグレナ藻綱
クロララクニオン藻綱
プラシノ藻綱
ペディノ藻綱
アオサ藻綱
トレボキシア藻綱
緑藻綱
車軸藻綱

28
2
2
4
3
6
1
19
1
3
1
1
1
12
1
19
3
1
6
1
2
14
52
16

68
3
2
19
5
10
1
27
1
3
1
1
1
16
1
38
6
1
11
1
5
24
130
60

445
4
6
40
7
42
1
46
1
3
1
2
1
39
1
58
7
1
23
1
6
57
275
148

貧膜口綱
ビコソエカ綱プラシディア綱

2
1
2

2
1
2

2
1
3

204

438

1,221

合計

分譲と寄託
過去10年間、NIESコレクションでは毎年400〜600株を研究や商業目的で分譲してきた。
Microcystis aeruginosa、Selenastrum capricornutum (現在はPseudokirchneriella subcapitataとして知られている)、Chlorella vulgaris、Anabaena flos-aquaeや Chattonella antiquaが過去5年間で最もよく分譲された種である。分譲された株は、AGPや生態毒性テスト、系統分類学的研究、新規生理活性物質の探求など、様々な分野の研究に用いられている。
NIESコレクションは、また、寄託も受け付けている（第Ⅱ章参照）。寄託された株は原則として全て、系統保存株評価委員会の評価の後NIES株番号が付され、公開される。
分譲依頼があると、新しい培地に植え継がれ、依頼者に郵送ないし宅配便で送られる。
NIESコレクションでは、NIES株を使った研究論文を出版した場合、別刷の送付をお願いしている。参考文献の項に示されているように、これまでに多くの科学的に有用な研究成-2-

果が出版されている。
凍結保存
凍結保存は、カルチャーコレクションにおける培養株の長期保存には必須の手段である。
繰り返しの植え継ぎ作業がほとんどないことから、継代培養では常に危惧される細菌などの混入の危険を回避できる。また、細胞分裂を押さえることから、保存中の培養株の変質、
特に突然変異などによる遺伝的な変質を最小限におさえることができる。現在、NIESコ
レクションには300余りのシアノバクテリア株と単細胞性紅藻株が液体窒素中の凍結保存（気相保存）のみで維持されている。Appendix I を参照されたい。
分類学的再検討
コッコイドの緑藻は、近年、鞭毛装置構造などの微細構造、18Sリボソーム遺伝子やrbcL遺伝子などの塩基配列解析によって分類学的位置の再検討が行われている。一部の目が改訂されたり、トレボキシア藻綱が設立されたりするなど、分類体系の再検討がなされる一方、科や属レベルでの多系統性が指摘されるようになった。そこで、NIESコレク
ションにおいても18Sリボソーム遺伝子の解析を独自に行い、一部の株を緑藻綱からトレ
ボキシア藻綱に移行した。また、この解析の過程で、不適当な種名または分類学的位置にある藻類株が見つかった。これらは、寄託者による誤同定か、寄託当時の分類学情報の欠如によるものであると考えられるが、本リストではコメントをつけてそのままにした。今後の分類学的研究に託したい。
シアノバクテリア株について
水の華形成藻類はNIESコレクションの特徴ともなっており、多くの分類学的研究がNIES 株 を 用 い て 行 わ れ て い る 。 そ の 中 で Microcystis 属 の 5 種 [M. aeruginosa (Kützing)
Lemmermann、M. ichthyoblabe Kützing、M. novacekii (Komárek) Compère、M. viridis (A.
Braun) Lemmermann、M. wesenbergii Komárek] をM. aeruginosaに統合することが提案されている（第Ⅹ章参照）。NIESコレクションではこれを支持し、Microcystis属の5種のうちNIESコレクションで保存する株をM. aeruginosaと表示し、前名をデータ中に示した。
他の保存施設との連携
NIESコレクションは、現在、英国のCCAP、フランスのPCC、米国のCCMP、ドイツのSAG、ノルウェーのNIVA（略称の正式名称は第Ⅶ章参照）と一部の株を共有している。
これらの株にはNIES番号が付してあるが、元の保存機関における株番号も各々の株デー
タ欄に記してある。このような保存機関同士の連携は、保存株を災害などで失わないため、
また、世界各地で微細藻類培養株の利用を可能にするために重要な活動である。NIESコ
レクションでは、このような他機関との連携をさらに拡大しようと考えている。

-3-

II. 培養株の寄託
１.

寄託条件

国立環境研究所微生物系統保存施設では基本的に次の条件を満たす株について寄託を受け付けています。寄託された株の受け入れの可否は微生物系統保存株評価委員会で決定します。寄託された株は、原則として、すべて分譲の対象になります。
(1) 環境問題にかかわる微生物、指標生物、タイプ株、有用な性質をもつ微生物、重要な研究で使われた微生物などの科学的に重要な微生物。
(2) 履歴が明らかであり、適正な種名のついた株であることを原則としますが、既に属名のみで多くの研究に使われている株は受け入れ対象とします。
(3) 保存条件が確立しており、安定した培養が可能な株であること。無菌培養株が望まし
いが、微細藻類の場合はクローン培養株か単藻培養株であること。原生動物の場合は、無菌株か餌としての生物のみが混入している単一種培養株、細菌類は純粋培養株であること。
(4) その他、微生物系統保存株評価委員会が特に必要と認めた株。

２.

寄託にあたっての同意事項

国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）は以下の同意事
項に同意していただいた方からの寄託を受け付けます。
(1) 寄託者は、寄託株を NIES コレクションに無償で寄託することとします。この寄託においては、知的所有権の移転は含まれません。寄託を受けて、NIES コレクションは、寄託された培養株の維持、保存、増殖を行い、また研究者に対し提供することができます。
(2) 寄託者は、寄託にあたって、寄託株の特性や品質に関する正確な情報（特許等を含む）を添付することとします（微生物株寄託依頼書兼同意書参照）。
(3) NIES コレクションに寄託するにあたり、寄託株は法律上あるいは契約上いかなる制限も受けていないものであり、その由来は以下のいずれかに該当することとします。
・寄託株は、寄託者が分離・開発した培養株である。
・他者が分離・開発した培養株であるが、寄託にあたっては分離・開発者の許可を得ている。
・寄託者が購入したものであるが、譲渡や寄託をすることについて制限を受けていない。

(4) NIES コレクションは寄託者の定める以下の条件で利用を希望する者へ寄託株を提供することができます。

-4-

・論文発表まで寄託株を公開・分譲しない。
・その他、寄託者の定める条件。
条件が付与されている場合でも、非公開は原則として寄託日から 1 年以内を目安とします。
また、条件が付与されていない場合は、寄託後、保存株評価委員会等の審査を経て直ちに公開・分譲します。

(5) 寄託者は、寄託株の維持・保存・増殖段階でのやむを得ない事情による変質・滅失あ
るいは自然災害その他の不可抗力による滅失・散逸などについて、NIES コレクショ
ンに対し責任を問うことはできません。
(6) NIES コレクションは、保存株評価委員会等の意見等を踏まえ、維持方針の変更が生じた場合は事前に寄託者に連絡のうえ、寄託株の維持･保存・提供の中止その他の処分をすることができます。

３.

寄託方法

「微生物株寄託依頼書兼同意書」（6〜8 頁）に必要事項を記入し、以下の宛先に郵送してください。ファクスまたは PDF ファイルで送付いただく場合でも、署名捺印された上記「依頼書兼同意書」の原本を後日必ず郵送してください。実際の株の引き取り時期などについては、スタッフにおたずねください。
宛先：〒305-8506

つくば市小野川16-2

国立環境研究所微生物系統保存施設
電話：029-850-2556
ファクス：029-850-2587
電子メール：mcc@nies.go.jp
寄託された株を受領後、株の状態が「寄託依頼書」に記された内容と相違した場合、当施設の判断で株の受け入れを取り消すことがあります。

-5-

微生物株寄託依頼書兼同意書
寄託依頼年月日：
依頼者名（フリガナ付）：
所属機関名（日本語名）：
所属機関名（英語名）：
所属機関住所：〒
電話：
FAX：
Eメールアドレス：

年

月

（内線

日

）

下記微生物の寄託を依頼します。
寄託理由：[基本情報]
学名及び命名者名：
門名：
綱名：
目名：
科名：
シノニム：
同定者名（フリガナ付）：
同定年：
年
月
日
株番号又は符号：
他の寄託先情報（同じ株を別の機関に寄託している場合，機関名と保存株番号を記述してくだ
さい）：

[採集]
採集年月日：年
月日
採集者名（フリガナ付）：
採集地情報
国名：
産地住所（県名から）：
地名（河川，湖，池，湾，砂浜等の名称）：
緯度経度：
海域名と最も近い国名：
生息環境：□陸域 □海域 □汽水域(塩濃度:‰)
生息環境の詳細：□貧栄養 □中栄養 □富栄養 □腐植栄養 □表層 □クロロフィル極大水深□その他の水深(−m) □その他()
陸水環境の詳細：□湖沼 □河川 □湿原 □湿地 □水田 □塩水 □土 □温泉 □鉱泉 □雪または氷□その他()
海水環境の詳細：□海浜域 □沿岸域 □外洋域 □潮だまり（タイドプール） □潮間帯 □干潟□マングローブ □河口 □港湾 □漂着（打ち上げ） □ドレッジ □その他()
生息環境コメント：[分離]
分離年月日：年
月日
分離者名（フリガナ付）：
分離試料源：□水 □海水 □砂 □泥 □底泥 □土 □植物 □海藻 □海草 □動物 □サンゴ □スポンジ□雪または氷 □その他()
分離時の状態：□運動性栄養細胞 □非運動性栄養細胞 □休眠細胞 □胞子 □四分胞子 □果胞子
□接合子 □単為発生配偶子 □葉状体 □その他()
分離方法：□ピペット洗浄法 □切り出し □希釈法 □寒天平板法 □走性 □セルソーター
□その他(
)

-6-

分離時の培養条件（培地の種類，濃度，光，温度条件等）が後述の保存条件と異なる場合，その詳細を記述してください：
分離時の処理：□無処理 □抗生物質(名称：，mg/l) □紫外線照射□化学物質(名称：，mg/l) □熱処理 □集積強化培養 □超音波処理□酸化ゲルマニウム □その他()[状態]
藻類・シアノバクテリア:

バクテリア:
原生動物:
最新の無菌検査年月日：

□単藻
□クローン
□無菌
□純粋
□無菌
□混合

□混合
□非クローン□非無菌□混合□二者培養 (餌生物:
年

月

)
日

[保存条件]
保存形態：□継代培養 □凍結保存 □両者併用 □その他()
培地名：
培地の出典：
培地形状：□液体 □半固体 □固体 □二相 □その他()
培地作成等の特記事項：
継代培養による保存条件
温度（℃）：
光強度（Lux）：
光強度（μE/m2sec）：
光質：□白色蛍光灯 □赤色蛍光灯 □青色蛍光灯 □自然光 □その他()
明暗周期：
継代培養（植え継ぎ）周期：（□日 □月間隔）
培養条件や植え継ぎ操作に関する特記事項（前培養の条件，特別な処理，最大の増殖率を得る条件，
細胞の接種方法や接種量など）：
凍結保存：□可 □否 □不明
凍結保護剤名：(濃度％）：
保存温度（℃）：
凍結方法：
凍結乾燥保存：□可 □否 □不明
L-乾燥保存：□可 □否 □不明[特性]
環境上の特性□赤潮形成 □水の華形成 □AGP試験 □生物指標 □水の華形成藻の捕食 □異味 □異臭□浄水障害 □毒性 □高CO2固定 □有害物質分解 □生分解活性 □酸化池 □汚泥 □生物膜処理□腐食性 □その他()
生理生態的特性□独立栄養 □混合栄養 □摂食栄養 □従属栄養 □浮遊性 □底生 □共生 □寄生 □腐生□コスモポリタン □広温性 □狭温性 □好熱性 □好冷性 □広塩性 □狭塩性 □好塩性 □好酸性
□陽生型 □ 陰生型 □窒素固定 □発酵 □生物発光 □走光性 □水素発生 □オイル生産□内生植物的生活 □植物着生 □ 岩石着生 □その他()
その他の特性□分類学上重要な株 □タイプ株 □運動性 □非運動性 □休眠胞子形成 □休眠胞子非形成 □色順応
□突然変異株 □雌雄異株 □雌雄同株 □雌雄異体 □雌雄同体 □同型配偶 □異型配偶 □卵生殖
□H,h 型生活環 □ H,d型生活環 □ D,d+h型生活環 □倍数性株 □交配型(＋) □交配型(−)□雌 □雄 □その他()-7-

[遺伝子データ（随時コピーを行い，登録されている全てのデータを記入してください）]
遺伝子名：
登録番号：
登録者名（フリガナ付）：
登録年月日：
年月日[文献]
この株を扱った文献（以下の例に従って記述してください）（例）Otsuka, S., Suda, S., Shibata, S., Oyaizu, H., Matsumoto, S. & Watanabe, M.M. 2001. A proposal for theunification of five species of the cyanobacterial genus Microcystis Kützing ex Lemmermann 1907 under
the rules of the Bacteriological Code. Int. J. Syst. Evol. Microbiol., 51, 873-879.

この株に関する参考文献（同定に用いた文献など，上の例に従って記述してください）

[その他]
コメント：

寄託にあたって以下の同意事項に同意いたします。
年
氏名：

月

日
印（又はサイン）

寄託にあたっての同意事項
1. 寄託者は、寄託株をNIESコレクションに無償で寄託することとします。この寄託においては、知的所
有権の移転は含まれません。寄託を受けて、NIESコレクションは、寄託された培養株の維持、保存、増殖を行い、また研究者に対し提供することができます。
2. 寄託者は、寄託にあたって、寄託株の特性や品質に関する正確な情報（特許等を含む）を添付することとします（寄託依頼書）。
3. NIES コレクションに寄託するにあたり、寄託株は法律上あるいは契約上いかなる制限も受けていないものであり、その由来は以下のいずれかに該当することとします。（該当するものを■とする。）□ 寄託株は、寄託者が分離・開発した培養株である。
□ 他者が分離・開発した培養株であるが、寄託にあたっては分離・開発者の許可を得ている。
□ 寄託者が購入したものであるが、譲渡や寄託をすることについて制限を受けていない。
□ その他（）
4. NIES コレクションは寄託者の定める以下の条件で利用を希望する者へ寄託株を提供することができます。（該当するものを■とする。）□ 論文発表まで寄託株を公開・分譲しない。
□ その他（）
条件が付与されている場合でも、非公開は原則として寄託日から 1 年以内を目安とします。また、条件が付与されていない場合は、寄託後、保存株評価委員会等の審査を経て受理後直ちに公開・分譲します。
5. 寄託者は、寄託株の維持・保存・増殖段階でのやむを得ない事情による変質・滅失あるいは自然災害
その他の不可抗力による滅失・散逸などについて、NIESコレクションに対し責任を問うことはできません。
6. NIESコレクション は、保存株評価委員会等の意見等を踏まえ、維持方針の変更が生じた場合は事前に寄託者に連絡のうえ、寄託株の維持･保存・提供の中止その他の処分をすることができます。

-8-

III.
１.

保存株の分譲

分譲にあたっての同意事項

国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）は、以下の事項に同意していただいた方に培養株を分譲します。
(1) NIES コレクションから分譲された微生物培養株、それを増殖させたもの及び由来物（以後培養株等と記す）は教育、試験・研究および製品等の開発目的でのみ使用できます。人に直接使用することはできません。また、利用者は使用の際に、分譲された培養株に潜在的な危険性があることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。特に有毒物質を生産する株については、使用中の管理に責任を持ち、使用後は株及び由来物を適切な方法で処理しなければなりません。
(2) 分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。
(3) 培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。
(4) 利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。
(5) 利用者は分譲された培養株等を第三者に分与または販売することはできません。
(6) NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し（例：NIES-123）、国立環境研究所微生物系統保存
施設に保存されている株であることを明記してください。分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。
(7) 分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。
(8) 分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は、利用者自らの責任で処理しなくてはなりません。
(9) 利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。
ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

-9-２.

株の注文方法(1) 「微生物株分譲依頼書兼同意書」（12 頁）に必要事項を記入し、以下の宛先に郵送してください。依頼者が学生、非常勤職員などの場合は、指導教官、雇用者などから依頼するようお願いします。株は指導教官、雇用者宛に郵送されます。また、お急ぎの場合は、分譲依頼のみをあらかじめファクス、PDF ファイルまたは電子メールでお送りください。なお株は署名捺印された依頼書兼同意書原本の到着確認後でないと発送できませんので、この点くれぐれもご注意ください。
(2) 保存株を受け取った方は、受領後 1 ヶ月以内に「微生物株受領報告書」（18 頁）に必要事項を記入し、以下の宛先にファクス、郵送、または電子メールで連絡してください。
宛先：〒305-8506

つくば市小野川16-2

国立環境研究所微生物系統保存施設
電話：029-850-2556
ファクス：029-850-2587
電子メール：mcc@nies.go.jp

３.

株の価格

約 15mL の培養液に植え込まれた培養株１本につき、非営利組織：6,000 円、営利組
織：10,000 円となります。この他に消費税と郵送料がかかります。小、中、高等学校の学
校教育用、また、大学であっても教育目的で使用する場合は無料です。ただし、これらの場合、種類の選択は保存施設にお任せいただきます。

４. “Untransportable”（輸送が困難な）株と凍結保存株の分譲についての注意“Untransportable” と記されている株は、保存株の状態や季節によって分譲依頼を受け付けられない場合があります（第Ⅶ章を参照のこと）。原則的に、これらの株はとりに来ていただくことになります。スタッフにご相談ください。
また、“[Cryopreserved]” と記されている株は、凍結保存されている株を解凍後、培養してからお送りします。したがって株を発送するまでに１ヶ月くらいかかる場合があります（Appendix I も参照のこと）。

- 10 -

５.

1993 年以降採集された外国産株の取り扱い

1993 年１月以降に外国より採集された株については、リストに掲載されていても生物多様性条約に関連して当面分譲を見合わせているものがあります。分譲依頼に際しては必ず保存株リストで産地および採集年をご確認の上、本項に該当すると思われる場合にはスタッフにお問合せください。

６.

有毒物質を生産する株の取り扱い

現時点で有毒物質を生産することがわかっている培養株は “Toxic” と記されています（Appendix II も参照のこと）。これらの株の分譲を希望する場合は、「有毒株分譲依頼書兼同意書」（13 頁）を提出してください。

７.

国立環境研究所の職員、客員研究員、共同研究員への分譲

それぞれに該当する「微生物株分譲依頼書兼同意書」（14〜17 頁）に必要な事項を記入し、署名捺印のうえ所内便で送付するか、微生物系統保存施設のスタッフに直接渡してください。お急ぎの場合は、分譲依頼のみ電子メール（mcc@nies.go.jp宛）で受け付けます。後日上記依頼書を必ずお持ちください。保存株は無料です。

- 11 -

微生物株分譲依頼書兼同意書（一般用）
分譲依頼年月日：
年
月日
依頼者名 (フリガナ)：（
所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：請求先：(上記機関、住所等と異なる場合にご記入ください)）

下記微生物についての分譲を依頼します。
微生物学名（NIES株番号）：
研究目的（できるだけ詳しくご記入ください）：

合計株数：
株データシートの必要な株番号：
分譲依頼にあたって以下の同意事項に同意いたします。
年
月
日
氏名：

印（又はサイン）分譲依頼者が学生、非常勤職員等の場合、指導教官又は責任者は以下の欄にご記入ください。
年月日
指導教官又は責任者名：
所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：

印（又はサイン）分譲にあたっての同意事項
1.

2.
3.
4.
5.
6.

7.
8.

9.

国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）から分譲された微生物培養株、それを増殖させたもの及び由来物（以後培養株等と記す）は教育、試験・研究および製品等の開発目的でのみ使用できます。人に直接使用することはできません。また、利用者は使用の際に、分譲された培養株に潜在的な危険性があることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。
分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。
培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。
利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。
利用者は分譲された培養株等を第三者に分与または販売することはできません。
NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し（例：NIES-123）、国立環境研究所微生物系統保存施設に保存されている株であることを明記してください。
分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。
分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。
分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は利用者自らの責任で処理しなくてはなりません。
利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

- 12 -

有毒株分譲依頼書兼同意書（一般用）
分譲依頼年月日：
年
月日
依頼者名 (フリガナ)：（
所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：請求先：(上記機関、住所等と異なる場合にご記入ください)）

下記微生物についての分譲を依頼します。
微生物学名（NIES株番号）：
研究目的（できるだけ詳しくご記入ください）：

合計株数：
株データシートの必要な株番号：
分譲依頼にあたって以下の同意事項に同意いたします。
年
月
日
氏名：

印（又はサイン）分譲依頼者が学生、非常勤職員等の場合、指導教官又は責任者は以下の欄にご記入ください。
年月日
指導教官又は責任者名：
所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：

印（又はサイン）分譲にあたっての同意事項
1．

2．
3．
4．
5．
6．

7．
8．

9．

国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）から分譲された微生物培養株、それを増殖させたもの及び由来物（以後培養株等と記す）は教育、試験・研究および製品等の開発目的であり、公共の安全が保証される目的でのみ使用できます。人に直接使用することはできません。また、利用者は、使用の際に、分譲された培養株が有毒物質を生産する株であることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。利用者は使用中の管理に責任を持ち、使用後は培養株をオートクレーブなどで死滅させ、由来物質については適切に処理しなければなりません。
分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。
培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。
利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。
利用者は分譲された培養株等を第三者に分与または販売することはできません。
NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し(例：NIES-123)、国立環境研究所微生物系統保存施設に保存されている株であることを明記してください。
分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。
分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。
分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は利用者自らの責任で処理しなくてはなりません。
利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸
送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

- 13 -

微生物株分譲依頼書兼同意書（国立環境研究所職員用）
分譲依頼年月日：
依頼者名 (フリガナ)：
所属部署：

年

月

日
（

）

領域/プロジェクト/センター/ラボ
研究室/チーム
電話：
FAX：
Ｅメールアドレス：
下記微生物についての分譲を依頼します。
微生物学名（NIES株番号）：
研究目的（できるだけ詳しくご記入ください）：

研究課題名：
研究課題コード：
合計株数：
株データシートの必要な株番号：
分譲依頼にあたって以下の同意事項に同意いたします。
年
月
日
氏名：

印（又はサイン）分譲にあたっての同意事項
1.

国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）から分譲された微生物培養株、それを増殖させたもの及び由来物（以後培養株等と記す）は教育、試験・研究および製品等の開発目的でのみ使用できます。人に直接使用することはできません。また、利用者は使用の際に、分譲された培養株に潜在的な危険性があることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。

2.

分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。

3.

培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。

4.

利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。

5.

利用者は分譲された培養株等を第三者に分与または販売することはできません。

6.

NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し（例：NIES-123）、国立環境研究所微生物系統保存施設に保存されている株であることを明記してください。
分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。

7.

分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。

8.

分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は利用者自らの責任で処理しなくてはなりません。

9.

利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

- 14 -

有毒株分譲依頼書兼同意書（国立環境研究所職員用）
分譲依頼年月日：
依頼者名 (フリガナ)：
所属部署：

年

月
（

日
）

領域/プロジェクト/センター/ラボ
研究室/チーム
電話：
FAX：
Ｅメールアドレス：
下記微生物についての分譲を依頼します。
微生物学名（NIES株番号）：
研究目的（できるだけ詳しくご記入ください）：

研究課題名：
研究課題コード：
合計株数：
株データシートの必要な株番号：
分譲依頼にあたって以下の同意事項に同意いたします。
年
月
日
氏名：

印（又はサイン）分譲にあたっての同意事項
1． 国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）から分譲された微生物培養株（及びそれを増殖させたものを含み、以後培養株等と記す）は教育、試験・研究および製品等の開発目的であり、公共の安全が保証される目的でのみ使用できます。人に直接使用することはできません。また、利用者は、使用の際に、分譲された培養株が有毒物質を生産する株であることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。利用者は使用中の管理に責任を持ち、使用後は培養株をオートクレーブなどで死滅させ、由来物質については適切に処理しなければなりません。
2． 分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。
3． 培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。
4． 利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。
5． 利用者は分譲された培養株等を第三者に分与または販売することはできません。
6． NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し（例：NIES-123）、国立環境研究所微生物系統保存施設に保存されている株であることを明記してください。
分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。
7． 分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。
8． 分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は利用者自らの責任で処理しなくてはなりません。
9． 利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

- 15 -

微生物株分譲依頼書兼同意書（客員研究員・共同研究員用）
分譲依頼年月日：
年
依頼者名 (フリガナ)：
所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：
国立環境研究所の受け入れ職員名：
所属部署：

月
（

日
）

下記微生物についての分譲を依頼します。
微生物学名（NIES株番号）：
研究目的（できるだけ詳しくご記入ください）：

合計株数：
株データシートの必要な株番号：
分譲依頼にあたって以下の同意事項に同意いたします。
年
月
日
氏名：

印（又はサイン）分譲依頼者が学生の場合は以下の欄も記入してください。
年月日
受け入れ職員名：

印（又はサイン）分譲にあたっての同意事項
1.

2.
3.
4.
5.
6.

7.
8.

9.

国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）から分譲された微生物培養株、それを増殖させたもの及び由来物（以後培養株等と記す）は教育、試験・研究および製品等の開発目的でのみ使用できます。人に直接使用することはできません。また、利用者は使用の際に、分譲された培養株に潜在的な危険性があることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。
分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。
培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。
利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。
利用者は分譲された培養株等を第三者に分与または販売することはできません。
NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し（例：NIES-123）、国立環境研究所微生物系統保存施設に保存されている株であることを明記してください。
分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。
分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。
分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は利用者自らの責任で処理しなくてはなりません。
利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

- 16 -

有毒株分譲依頼書兼同意書（客員研究員・共同研究員用）
分譲依頼年月日：
年
依頼者名 (フリガナ)：
所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：
国立環境研究所の受け入れ職員名：
所属部署：

月
（

日
）

下記微生物についての分譲を依頼します。
微生物学名（NIES株番号）：
研究目的（できるだけ詳しくご記入ください）：

合計株数：
株データシートの必要な株番号：
分譲依頼にあたって以下の同意事項に同意いたします。
年
月
日
氏名：

印（又はサイン）分譲依頼者が学生の場合は以下の欄も記入してください。
年月日
受け入れ職員名：

印（又はサイン）分譲にあたっての同意事項
1． 国立環境研究所微生物系統保存施設（以後 NIES コレクションと記す）から分譲された微生物培養株（及びそれを増殖させたものを含み、以後培養株等と記す）は教育、試験・研究および製品等の開発目的であり、公共の安全が保証される目的でのみ使用できます。人に直接使用することはできません。また、利用者は、使用の際に、分譲された培養株が有毒物質を生産する株であることを認識し、その国、自治体、機関等の法令や規則を遵守しなければなりません。利用者は使用中の管理に責任を持ち、使用後は培養株をオートクレーブなどで死滅させ、由来物質については適切に処理しなければなりません。
2． 分譲を希望する場合は、利用者本人が分譲依頼書を提出してください。
3． 培養株等に関する知的所有権等が、分譲によって利用者へ与えられるものではありません。
4． 利用者が分譲時に示した使用目的から大幅に異なる目的に使用する場合、利用者はその旨を NIES コレクションに書面で連絡しなくてはなりません。
5． 利用者は分譲された培養株等を第三者に分与または販売することはできません。
6． NIES コレクションから分譲された株を利用した成果を発表する場合、番号の前に必ず NIES-をつけた株番号を記し（例：NIES-123）、国立環境研究所微生物系統保存施設に保存されている株であることを明記してください。
分譲された株を利用して論文発表した場合は別刷りまたはコピーを２部、NIES コレクションに送ってください。
7． 分譲された培養株等の使用が第三者の知的所有権やその他の権利を侵害していた場合、利用者は利用者の責任によって対処しなければなりません。
8． 分譲された培養株等が、欠点、危険な特性、不具合等を有している可能性があること、あるいは特定の目的に合致しているとは限らないことを認識し、分譲された培養株等の利用によって損失が生じた場合は、利用者は利用者自らの責任で処理しなくてはなりません。
9． 利用者は、培養株を受領後、1 ヶ月以内に受領報告書を NIES コレクション宛に提出しなくてはなりません。輸送中の環境条件の変化などにより、その時点で増殖状態が悪い場合、利用者は NIES コレクションに無償で再分譲を要求することができます。ただし、NIES コレクションはその期間内であっても、利用者の過失に対しての責任は負いません。

- 17 -

微生物株受領報告書
受領報告年月日：

年

月

依頼者名 (フリガナ)：

日（）

所属機関名：
郵便番号：〒
住所：
電話：
FAX：
Ｅメールアドレス：平成年

月日に分譲された微生物株の受領と受領時の株の状態について下記のように報告します。

受付番号：
微生物学名（NIES株番号）：

株が到着した日付：

年月

株の受領時の状態
良

好：

不

良：

その他：

ご意見、要望など：- 18 -

日

IV. 分譲株の培養保存法
微生物株は、ねじ口試験管に培養された状態で郵送される。株の分譲を受けた場合、株を絶やさないために下記の点に留意する必要がある。
i)

培地は株を受け取る前に作成しておく。

ii)

株を受領後速やかに荷をとき、新鮮な培地に植え継ぎ、当方で指示した温度と光強度下（第Ⅷ章参照）で培養する。その場合明暗サイクルは12時間明期12時間暗期とし、ねじ口試験管のねじ蓋をゆるくする。
iii)

良好な増殖が確認された後に、更に株を保存する場合には、当方で指示した期間

毎に新鮮な培地に移植する必要がある（第Ⅷ章参照）。

V. 藻類培地作成の基本手法
藻類株の保存には、数多くの培地を必要とする。それぞれの培地は次章に掲載した処方せんに従って作成されるが、正確かつ簡便に培地を作成するために、本施設で採用している基本手法について述べておきたい。

１.

保存試薬液

培地は一般に多量栄養素、微量金属、およびビタミン類(表２)で構成されている。これ
らの諸成分の保存試薬液を作成しておくことが、培地作成の簡便さをもたらす。このうち微量金属やビタミン類の保存液の濃度は非常に低いので、保存試薬液作成時には、より濃度の高い原液を作成する必要がある。以下、各々について保存試薬液の濃度と作成方法について述べる。
Ａ

多量栄養素：各栄養素につき、10mg/mlの濃度の保存試薬液を別々に作成し、冷蔵庫(5℃)で保管する。
Ｂ

微量金属：これらの成分は、各種の保存試薬液として別々に作成され保管される場

合と、混液で保管される場合がある。
(1) 各種保存試薬液i)10〜100mg/mlの濃度で各種金属の原液を作成する。

ii)

各原液を1mg/mlの濃度に希釈し冷蔵庫(5℃)に保管する。

- 19 -

表２. 培地に使われる各種栄養素＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿
多量栄養素

微量金属

NaCl

H3BO3

KCl

MnCl2・4H2O

CaCl2・2H2O

MnSO4・7H2O

MgCl2・6H2O

FeCl3・6H2O

Na2SO4FeSO4・7H2O

K2SO4

CoCl2・6H2O

MgSO4・7H2O

ZnSO4・7H2O

NaNO3

CuSO4・5H2O

KNO3

Na2MoO4・2H2O

Ca(NO3)2・4H2O

ビタミン類

NH4NO3

Vitamin B12

NaH2PO4・2H2O

Biotinβ-Na2 glycerophosphate・5H2O

Thiamine HCl

KH2PO4

Nicotinic acidK2HPO4

Calcium panthothenate

Na2CO3

p-Aminobenzoic acid

NaHCO3

Inositol

Na2SiO3・9H2O

Folic acid
Thymine＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿(2) 混液i)(1)-i)と同様の操作を行う。

ii)

必要量の80％の蒸留水をビーカーに加える。

iii)

十分に撹拌しながら必要量のNa2EDTAを溶解する。

iv)

十分に撹拌しながら各種微量金属原液を必要量添加する。

v)

蒸留水を加え、最終量に調整し、冷蔵庫(5℃)に保管する。
ビタミン類：ビタミンＢ12、ビオチン、チアミンの３種のビタミンだけで多くの藻Ｃ類が増殖するので、殆どの培地はこれら３種のビタミン類だけが添加されている。しかし、いくつかの培地では、他のビタミン類が添加されている。
(1) ビタミンＢ12、ビオチン、チアミンi)

ビタミンＢ12とビオチンについては、各々0.1mg/mlの原液を作成し、チアミンについては10mg/mlの原液を作成する。

- 20 -

ii)

これらの原液を多数の試験管に1mlずつ分注し、オートクレーブ滅菌(121℃,

20min)後、−20℃のフリーザーに保管する。
iii)

各ビタミンについて、保存原液の1mlを融解し、蒸留水で1/100に希釈してビタミ

ンＢ12、ビオチンについては1µg/mlの保存試薬液、チアミンについては、100µg/mlの保存試薬液を作成し、冷蔵庫に保管しながら使用する。
(2) 他のビタミン類：ある培地では、多種のビタミン類が混液の形で添加される(第Ⅵ章-64参照)。大量に作成しておくことをすすめる。
i)

各種のビタミンについて0.1〜1mg/mlの原液を作成する。

ii)

必要量の80％の蒸留水をビーカーに加える。

iii)

十分に撹拌しながら各種ビタミンを必要量加える。

iv)

蒸留水で最終量に調整する。

v)

ミリポアフィルター(0.22μm)でろ過滅菌したのち、滅菌された試薬瓶に100mlずつ分注し、−20℃のフリーザーで保管する。一部を融解し、冷蔵庫(5℃)に保管しながら使用する。

２.

培地作成

培地は、合成培地と強化培地に大別される。すべての淡水藻や一部の海産藻は合成培地
で、殆どの海産藻は強化培地で保存されている。
(1) 合成培地(淡水)i)

必要量の80〜90％の蒸留水をビーカーに加える。

ii)

十分に撹拌しながら、Tris、glycylglycine、HEPES、TAPS、Bicine、MES等の緩衝剤(必要とされる場合)を必要量天秤で秤量し、添加する。
iii)

各種栄養塩を各々の保存液から必要量添加する。

iv)

蒸留水で最終量に調整する。

v)

緩衝剤が使用されている場合、1N HClあるいは、1N NaOHで、使用されていな

い場合は各々1/10の濃度でpHを調整する。
vi) 培地10mlずつ試験管(18×150mm)に分注し、オートクレーブで滅菌する（121℃,
20min)。
(2) 合成培地(海水)i)

必要量の80％の蒸留水をビーカーに加える。

ii)

十分に撹拌しながら、緩衝剤(Tris,NTA等)および多量栄養塩類(NaCl,MgSO4・

7H2O,KCl,CaCl2・2H2O)を必要量天秤で秤量し、添加する。
iii)

他の各種栄養塩を各々の保存液から必要量添加する。

iv)

蒸留水で最終量に調整する。

- 21 -

v)

1N HClでpHを調整する(通常8.0)。

vi)

培地10mlずつ試験管に分注し、オートクレーブで滅菌する(121℃, 20min)。

(3) 強化海水培地
i)

汚染のない外洋海水を採取し、ワットマンGF/Cフィルターでろ過し、粒子を除く。
ii)

塩分を調べる。通常の外洋海水の塩分は約35‰である。

iii)

必要量の80〜90％の海水をビーカーに加える。

iv)

必要量のTris等の緩衝剤を天秤で秤量し、溶解する(必要とされる場合)。

v)

他の栄養塩類を各々の保存液から必要量添加する。

vi)

海水で最終量に調整する。

vii)

pHを測定する。指示されている場合は1N HClで調整する(通常8.0)。

viii)

培地10mlずつ試験管に分注し、オートクレーブで滅菌する(121℃, 20min)。

３.

寒天斜面培地

通常寒天は1.5％の濃度で滅菌する前に液体培地に加えられる。
i)

寒天を必要量天秤で秤量し、液体培地に添加し、オートクレーブで121℃に熱し、

溶解する。
ii)

溶解後、速やかに10mlずつ試験管に分注し、オートクレーブで滅菌する(121℃,

20min)。
iii)

滅菌後、試験管上部に直径1cmの枕木をして寝かせ、放冷して培地を斜面状に固まらせる。

- 22 -

PREFACE TO THE SEVENTH EDITION
Four years have past since we published the sixth edition of the list. During this period a
considerable number of new cultures have been added to the NIES-Collection. We appreciate themany comments and words of encouragement about the publications from people in diverse places.
These have led us to recognize more than ever the value of the NIES-Collection for research anddevelopment. Its use extends not only to environmental science, but also to basic biology andmicrobiology-related fields such as agriculture, fisheries, food manufacture and medical science.
The seventh edition lists 1,215 strains of microalgae and 6 strains of protozoa. These have been
evaluated by the Committee for Evaluating Microbial Culture Strains, which is composed of
microbiologists from this institute and authorities from other organizations. Although special carehas been exercised to ascertain that the taxonomy and characteristics of all strains are clear andprecise, we are always grateful for further advice and criticism.
Most of the strains in the NIES-Collection were isolated originally by phycologists in ourcountry and do not exist in other collections. We plan to share responsibility for preservation of theimportant strains by keeping close contacts with other culture collections.
The NIES-Collection carries out such wide-ranging activities as collection, preservation,
distribution, taxonomy, preservation technology, and development of a microbial strain dataprocessing system. We hope to make steady progress in these various activities by expansion offacilities and staff. We would much appreciate your advice, criticism and cooperation concerning theperformance of the NIES-Collection.
I wish to express my sincere thanks to all of the members of the committee for their effort
devoted to the evaluation of microbial strains for deposit, and their numerous considerations andsuggestions for this publication. I would also like to pay my respect to the staffs of the NIESCollection for their enthusiasm for culture collection.

March 1, 2004

Makoto M. Watanabe, D.Sci.
Chairman for the Committee for
Evaluating Microbial Culture Strains
Director of Environmental Biology Division- 23 -

PREFACE TO THE FIRST EDITIONIn January 1983, the first culture collection of environmental microorganisms in Japan wasestablished at the National Institute for Environmental Studies. In the two years since that time, many
dedicated people have collaborated in the collection of microorganisms for the institute. The fruits of
their efforts have culminated in a "List of Strains," which I feel will be highly praised by environmental
scientists. I would like to extend to all who were involved, my most sincere thanks and gratitude.
The list published herein focuses on microalgae which are important primary producers in theenvironment.

Notwithstanding the fact that there has been a high demand for microalgal collections byboth the academic and industrial worlds, until the establishment of the NIES-Collection, no microalgal
culture collection for environmental studies per se existed in Japan. Unlike the culture collection ofbacteria and fungi, organisms which have been actively studied for a long time, the isolation, cultivation,and preservation of microalgae are technically much more complex.

Since this institution has

characteristically performed interdisciplinary studies, it was possible to conquer these difficulties, and set
the culture collection of microalgae on the right path by utilizing the knowledge of its many experts.
Users of the microbial strains of the NIES-Collection will find both their quality and the data
maintained about them, highly reliable because the characteristics of the microalgae have been carefully
examined and re-examined. Due to the development of the strain computer data processing system,
strain data have added to the general data base of environmental biology.

Collectively, thesedevelopments will contribute to the rapid growth of environmental microbiology, and allow it to catch up
with microbiological research in other fields.
Although the ultimate objective of the NIES-Collection is to collect and preserve a great variety of
microorganisms related to environmental problems, at present only the collection of microalgal cultures
has been established. I hope that in the future the NIES-Collection will preserve not only microalgae,
but also other microorganisms which are indispensable to environmental biology.

By planning

expansion of the facilities and the staff, the NIES-Collection should develop as an international culturecollection center, truly worthy of the name.

September 1, 1985

Tomomichi Yanagita, D. Sci.
Professor Emeritus of the University of Tokyo- 24 -

CONTENTS
Preface to the Seventh Edition ........................................ Makoto M. Watanabe
Preface to the First Edition........................................... Tomomichi Yanagita
Ⅰ．Introduction .................................................................. 27
Ⅱ．Deposition of Strains ........................................................... 30
１．Conditions for deposit ........................................................ 30
２．Agreement for deposit ........................................................ 30
３．Procedure for deposit ......................................................... 31
Ⅲ．Ordering and Distribution of Strains ............................................. 35
１．Agreement for distribution..................................................... 35
２．Order procedures ............................................................ 35
３．Price of strains .............................................................. 36
４．Special warning for distribution of "Untransportable" and "[Cryopreserved]" strains ....... 36
５．Special warning for distribution of strains collected outside of Japan since 1993........... 36
６．Special warning for "Toxic" strains .............................................. 37
７．Distribution to researchers belonging to the NIES .................................. 37
Ⅳ．Establishment of Fresh Cultures ................................................. 45
Ⅴ．Basic Methods for Preparation of Algal Culture Media .............................. 45
１．Stock solutions.............................................................. 45
２．Media ..................................................................... 47
３．Agar slant.................................................................. 48
Ⅵ．Media ....................................................................... 49
１．Stock media for algae ........................................................ 49
２．Bacteria-free check media ..................................................... 56
３．Trace metals, vitamin mixes and soil extract ....................................... 57
４．Stock medium for protozoa .................................................... 58
Ⅶ．Explanatory Notes about Strain Data ............................................. 61
Ⅷ．Strain Data ................................................................... 63
Appendix I. List of cryopreserved strains......................................... 188
Appendix II. List of toxic strains ............................................... 190
Appendix III. List of gene data ................................................. 191
Ⅸ．Indexes ..................................................................... 198
１．Numerical index............................................................ 198
２．Systematic index ........................................................... 211
Ⅹ．References .................................................................. 226- 25 -

I. INTRODUCTION
The Microbial Culture Collection at the National Institute for Environmental Studies (NIESCollection) was founded in 1983 within NIES.

When the NIES-Collection was created,environmental issues such as eutrophication of lakes and rivers, air and water pollution, and humanhealth problems caused by environmental pollution were much more severe than nowadays.

The

NIES-Collection started with ca. 250 strains mostly deposited by NIES scientists who were involvedin environmental research and by other culture collections in Japan (Ref. 526, 527).

Thus, red-tide-

forming algae, such as Chattonella antiqua and Heterosigma akashiwo, and water-bloom-forming
cyanobacteria, such as Microcystis aeruginosa were representatives at the start, and still characterize
the culture collection.

Strains maintained
During 20 years, the number of strains has increased (Fig. 1) by deposition from researchersand original isolation in the NIES-Collection. At present, the NIES-Collection holds more than
1,400 strains in total, including unidentified strains, and 1,221 strains (Table 1), which cover almost
all classes of algae so far described, are available to distribution as the NIES strains listed herein.
In the NIES-Collection, most strains are maintained under optimal and/or suboptimal
conditions ranging from 5 to 25 °C and 4 to 40 µmol /m2 sec light intensity in a 12-h-light:12-h-dark
photo-regime.

The strains are serially transferred at 10 days to 4 months intervals.

These

maintenance conditions differ depending on algal strains as described in Chap. VIII of this list. To
prevent loss of the strains during maintenance by serial transfer, we check growth weekly. Once a
year, a check for absence of bacteria is also carried out for the axenic strains by using several
bacterial check media (refer to Chap. V). Axenic strains are necessary for conducting some genetic
and physiological work.

Now, we have about 400 axenic strains.

Digital images and sequences of 18S rDNA are also routinely collected for evaluation of thestrains.

These data are reflected in the list published every 3 years (Ref. 521, 528, 529, 531, 532,
540, 541) or can be searched online(http://www.nies.go.jp/biology/mcc/home.
htm).

Fig. 1. Changes in strain numbers appearedin the List of Strains, from the first to
seventh editions. In this figure *Chlorophyceae includes Chlorophyceae sensu
stricto, Pedinophyceae, Ulvophyceae,
Trebouxiophyceae and Charophyceae.

- 27 -

Table1.

Number of genera, species and strains in the NIES-Collection.

PhylumClass

Cyanophyta (Cyanobacteria)
Glaucophyta
Rhodophyta
Cryptophyta
Heterokontophyta

Haptophyta
Dinophyta
Euglenophyta
Chlorrarachniophyta
Chlorophyta

Protozoa

Genus

Species

Strains

Cyanophyceae
Glaucophyceae
Rhodophyceae
Cryptophyceae
Chrysophyceae
Raphidophyceae
Dictyochophyceae
Bacillariophyceae
Phaeophyceae
Xanthophyceae
Pelagophyceae
Pinguiophyceae
Schizocladiophyceae
Prymnesiophyceae
Pavlophyceae
Diophyceae
Euglenophyceae
Chlorarachniophyceae
Prasinophyceae
Pedinophyceae
Ulvophyceae
Trebouxiophyceae
Chlorophyceae
Charophyceae

28
2
2
4
3
6
1
19
1
3
1
1
1
12
1
19
3
1
6
1
2
14
52
16

68
3
2
19
5
9
1
27
1
3
1
1
1
16
1
38
6
1
11
1
5
24
130
60

445
4
6
40
7
42
1
46
1
3
1
2
1
39
1
58
7
1
20
1
6
57
275
148

Oligohymenophorea
Bicoecea

2
1

2
1

2
1

2
204

2
438

3
1,221

Placididea
Total

Distribution and deposition
In the last 10 years, the NIES-Collection has distributed 400 to 600 strains a year to bothacademic and commercial users. Microcystis aeruginosa, Selenastrum capricornutum (currently
known as Pseudokirchneriella subcapitata), Chlorella vulgaris, Anabaena flos-aquae, andChattonella antiqua were the 5 most popular species in the last 5 years.

Distributed strains areused for various purposes, e.g. various tests to evaluate algal growth potential and ecotoxicity,
phylogenetic studies, characterization of physiological traits leading to the development of bioactive
compounds, and so on.
The NIES-Collection accepts the deposit of strains, all of which become available to all users
after evaluation by the Committee for Evaluating Microbial Culture Strains and allocation of a NIES
strain number.

Upon request, the strain is transferred to fresh medium, and shipped to the customer.

- 28 -

We ask customers to send reprints if they publish results based on our strains.

A lot of challenging

studies have been conducted using NIES strains, as seen in Chap. X.

Cryopreservation
Cryopreservation is necessary for long-term preservation in culture collections, preventing
contamination during repetitive transfer and genetic changes by mutation.

We have about 300

cyanobacterial and unicellular red algal strains preserved only in liquid nitrogen as listed inAppendix I.

Taxonomic re-evaluation
The taxonomic positions of coccoid green algae have been revised in recent years on the basisof the ultrastructure of the flagellar apparatus and DNA sequences. This revision has advanced thetaxonomy of green algae and led to the revision of several orders as well as the creation of a newclass.

However, as polyphyly in some families and genera is recognized, it is difficult to classify

those groups without sequence data. Moreover, this fact has directly affected the list of strains, inwhich classification to class level is necessary.

Thus, we started the sequencing of 18S rDNA to

increase the knowledge of culture strains in the NIES-Collection. As a result, some strains havebeen transferred from Chlorophyceae to Trebouxiophyceae.

In addition, we found several strains

assigned to inappropriate taxonomic status, e.g. taxonomic position and species names, in the courseof those analyses.
information.

It may be due to misidentification by depositors, or to paucity of phylogenetic

We left those strains as they were with comments.

Cyanobacterial strains
As mentioned above, bloom-forming cyanobacterial strains are characterizing our culturecollection, and taxonomic studies of cyanobacteria have been comprehensively carried out using
NIES strains (see Chap. X).

As a result of those taxonomic studies on Microcystis, fivemorphospecies of Microcystis (M. aeruginosa (Kützing) Lemmermann, M. ichthyoblabe Kützing, M.
novacekii (Komárek) Compère , M. viridis (A. Braun) Lemmermann, and M. wesenbergii Komárek
were unified into one species, M. aeruginosa.

The NIES-Collection accepted this proposal. In

Chap. VIII, however, former names are also shown in individual strain data.

Collaboration with other culture collections
Now, the NIES-Collection shares several strains with CCAP, PCC, CCMP, SAG and NIVA
(see Chap. VII for acronyms).
culture collections.

Those strains are shown with the strain numbers given by the former

We are planning to expand these collaborations to other culture collections as

well, for safety and to enlarge the availability of strains in the world.

- 29 -

II. DEPOSITION OF STRAINS
1. Conditions for deposit

The Committee for Evaluating Microbial Culture Strains at NIES (abbreviate to CEMCS
hereafter) has decided to accept for deposit strains that satisfy the following conditions as the NIES
strains maintained in the NIES-Collection. In principle, all deposited strains will be available to the
public.
(1) Scientifically important microorganisms, e.g. microorganisms which cause or remediate
environmental problems, bioindicators, type strains, microorganisms with useful physiologicaland biochemical properties, and established strains which have been used for valuable research.
(2) The background of the strain should be clarified, and the specific name should be established;however, strains that have been used in a number of studies may be accepted even if only thegeneric name is known.
(3) The strain should be stable under defined culture conditions and shall be in one of the following
states:・ Microalgae: clonal or unialgal strain (axenic strains are preferable)
・ Protozoa: axenic or xenic strain with supplemented microorganisms as food・ Bacteria: pure strain(4) Some other microorganisms may be accepted for deposit when the CEMCS recognizes theirimportance.

2. Agreement for deposit

When the depositor agrees to the following conditions, the NIES-Collection shall accept
his/her submission of the Strain Deposit Request and Agreement Form:(1) The depositor shall deposit the strain to the NIES-Collection without charge. The transfer ofintellectual properties is not included in the agreement. The NIES-Collection may maintain,
culture and distribute the strain to users.
(2) The depositors shall submit accurate strain data to the NIES-Collection, which shall include
patents, properties and states of the strain (see Strain Deposit Request and Agreement Form).
(3) The strain shall be free from any limitation, legally and contractually, pursuant to one of thefollowing reasons:・ The strain was isolated/developed by the depositor
・ The strain is deposited with the permission of the isolator/developer- 30 -

・ The strain has been purchased without any limitation regarding the deposit thereof(4) The NIES-Collection may distribute the deposited strains to users in accordance with thefollowing condition(s):・ The strain shall not be disclosed to the public until the paper regarding the strain has been
published・ Other reason specified by the depositor
This condition will last no longer than one year and the strain will be open to the public, even if
the depositor imposes conditions. If the depositor does not specify a condition, then the strain
will be open to the public immediately after approval by the CEMCS.
(5) The NIES-Collection shall bear no responsibility for inevitable change and loss during
maintenance, or for loss caused by natural disasters.
(6) The NIES-Collection may stop the maintenance and distribution of the strains according to adecision of the CEMCS, after prior notification is given to the depositor.

3. Procedure for deposit(1) The depositor should complete the “Strain Deposit Request and Agreement Form” (pp.32〜34)
and send it to the following address:
Microbial Culture Collection
National Institute for Environmental Studies,
16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Phone : +81-29-850-2556
Fax : +81-29-850-2587
Email: mcc@nies.go.jp(2) The decision regarding the deposit of the strain is given within one month from the date ofreceipt of the Strain Deposit Request and Agreement Form.
(3) The depositor should send an actively growing or lyophilized sample of the strain with twocopies of relevant reprint(s), if available, within one month of the date of acceptance.
(4) If the state of the strain sent does not coincide with the description on the Stain Deposit Request
and Agreement Form, or does not meet any of the conditions described above, the NIESCollection acceptance for deposit shall be canceled.

- 31 -

Strain Deposit Request and Agreement Form
Date:
Depositor's full name with family name in capital:
Depositor's affiliation and address:
Tel:
Fax:
E-mail:I wish to deposit the following microbial culture strain to the NIES-Collection.
Reason for deposit:[Basic information]
Scientific name with author name(s):Division:
Class:
Order:
Family:Synonym:
Identified by (full name with family name in capital):
Identification year:
Strain designation or code:
Other collection number (note collection name and number, if you deposit the strain in other collection):[Collection]
Collection date:
Collector's name (full name with family name in capital):
Site information
Country:
Address (most detailed one):
Place name (e.g. name of river, lake, pond, bay and coast):
Latitude and longitude:
Ocean name with a nearest country:
Habitats: □marine □freshwater (incl. terrestrial and aerial) □brackish (salinity:‰)
Details of habitats: □oligotrophic □mesotrophic □eutrophic □dystrophic □surface□depth in chlorophyll max □other depth (- m) □others ()
Details on the freshwater environment: □lake □pond □river □wetland □rice field□salt water □soil □rock □bark □hot spring □cold spring □snow or ice□others ()
Details on the marine environment: □shore □coastal □pelagic □tide pool □intertidal□tidal flat □mangrove □estuary □harbor □wrack □dredge sample□others ()
Other information or comments on the habitat:[Isolation]
Date of isolation:
Isolator's name (full name with family name in capital):
Isolation source: □water □seawater □sand □mud □sediment □soil □plant □seaweed□sea grass □animal □coral □sponge □snow □ice □others ()
Isolation objective □motile vegetative cell □non-motile vegetative cell □dormant cell □spore□tetraspore □carpospore □zygote □parthenogenetic gamete □thallus□others ()
Isolation method: □pipette washing □cut-out of specimen □dilution □agar plating □taxis□flow cytometry with cell sorter □others ()
Notes on isolation conditions (e.g. medium, light, temperature, if different from maintenance conditions):- 32 -

Isolation treatment: □none□chemicals (name:□GeO2 □others ([Strain status]
Algae and cyanobacteria:
Bacteria:
Protozoa:□antibiotics (name:,□unialgal□clonal□axenic□pure□axenic□mixedmg/l),mg/l) □ultra-violet irradiation□heat □ultra-sonic □enrichment culture)□mixed□non-clonal□non-axenic□mixed□monoxenic (food:)Date of axenic check:[Preservation conditions]
State of the preservation: □subculture □cryo-preservation □both □others ()
Medium:Reference of the medium:
Medium form: □liquid □semisolid □solid □soil water biphasic
Notes for preparation of medium:Sub-culturing conditions
Temperature (°C):
Light intensity （Lux）:
Light intensity （µE/m2 sec）:
Light quality: □white fluorescent □red fluorescent □blue fluorescent □natural light□others ()
L/D cycle:
Duration (day(s), month(s)):
Culture vessel: □test tube □Erlenmeyer flask □plastic culture flask□others ()
Additional notes for culture conditions (e.g. pre-culture conditions, special treatments, information for
optimal growth conditions, transfer methods, quantity of cells to transfer, others ()):
Cryopreservation: □yes □no □unknown
Cryoprotectant:(concentration
Preservation temperature (°C):
Method:Preservation in freeze-drying: □yes □no □unknown
Preservation in L-drying: □yes □no □unknown%)[Characteristics]
Environmental characteristics□red tide □water bloom □AGP □bioindicator □predator of water bloom forming species□offensive taste □offensive odor □clogging of purification □toxic□high CO2 fixing potential □decomposition of hazardous substances □biodegradation□oxidation pond □activated sludge □biofilim process □corrosion□others ()
Physiological and ecological characteristics□autotrophic □mixotrophic □phagotrophic □heterotrophic □planktonic □benthic□symbiotic □parasitic □saprophytic □cosmopolitan □eurythermal □stenothermal□thermophilic □cryophilic □euryhaline □stenohaline □halophilic □acidophilic□sun plant type □shade plant type □nitrogen fixation □fermentation □bioluminescence□phototaxis □hydrogen evolution □oil (hydrocarbon) production □endophytic □epiphytic□epilithic □others ()
Miscellaneous characteristics□taxonomic importance □type strain □motile □immotile □resting spore forming□resting spore not forming □chromatic adaptation □mutant □heterothallic □homothallic□dioecious □monoecious □isogamy □anisogamy □oogamy □life cycle (H, h type)□life cycle (H, d type) □life cycle (D, d+h type) □polyploidy □mating type(＋)□mating type(-) □female □male □others ()- 33 -

[Genetic information (please write all registered data)]
Gene name:
Accession no:
Registrant (full name with family name in capital):
Registration date:[References]
Publications in which the strains were used (please make a reference list according to an example below)(Example) Otsuka, S., Suda, S., Shibata, S., Oyaizu, H., Matsumoto, S. & Watanabe, M. M. 2001. A
proposal for the unification of five species of the cyanobacterial genus Microcystis Kützing ex
Lemmermann 1907 under the rules of the Bacteriological Code. Int. J. Syst. Evol. Microbiol., 51, 873879.

Other references relevant to the strain(s) (e.g. references used for identification, please make a reference
list according to an example above)[Others]
Any other remarks and comments:

I accept the following conditions for deposit of the strain(s).
Signature
Printed name

Date

Agreement for deposit
1. The depositor shall deposit the strain to the NIES-Collection without charge. The transfer ofintellectual properties is not included in the agreement. The NIES-Collection may maintain, culture
and distribute the strain to users.
2. The depositor shall submit accurate strain data to the NIES-Collection, which shall include patents,
properties and states of the strain.
3. The strain shall be free from any limitation, legally and contractually, pursuant to one of the following
reasons (please tick).
□ The strain was isolated/developed by the depositor□ The strain is deposited with the permission of the isolator/developer□ The strain has been purchased without any limitation regarding the deposit thereof
4. The NIES-Collection may distribute the deposited strains to users in accordance with the following
condition (please tick):□ The strain shall not be disclosed to the public until the paper regarding the strain has been
published□ Other reason ()
This condition will last no longer than one year and the strain will be open to the public, even if
the depositor imposes conditions. If the depositor does not specify a condition, then the strain
will be open to the public immediately after approval by the Committee for Evaluating Microbial
Culture Strains.
5. The NIES-Collection shall bear no responsibility for inevitable change and loss during maintenance,or for loss caused by natural disasters.
6. The NIES-Collection may stop the maintenance and distribution of the strains according to a decisionof the Committee for Evaluating Microbial Culture Strains, after prior notification is given to thedepositor.

- 34 -

III. ORDERING AND DISTRIBUTION OF STRAINS
1. Agreement for distribution

The NIES-Collection will distribute strains to users who agree to the following conditions:(1) The strains (including replicates and derivatives from the strains), which are distributed fromthe NIES-Collection, shall be available for education, research, and development purpose only.
The strains are not intended to apply directly to humans.

The user hereby acknowledges andaccepts the potential risks of the strains and shall use them in compliance with domestic andforeign laws, regulation, and guidelines. Especially for strains that produce toxic substances,
the user shall store and discard them appropriately.
(2) The user shall be requested to submit the application form personally.
(3) The user shall not acquire any intellectual property rights by the purchase of the strain.
(4) The user shall provide written notice to the NIES when the purpose has changed considerablyfrom the purpose that was stated at the time of submission.
(5) The user shall not distribute the strains, replicates and derivatives to any third party.
(6) The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or
photocopies thereof to the NIES-Collection.
(7) When the use of the strain violates another person’s rights, the user shall bear responsibility
therefor, and deal with the matter on its own.
(8) The user shall acknowledge the possibility that the strain is deficient and harmful, andinadequate to the user’s aim.

Thus, if the user suffers any loss by the strain, he/she shall bear

responsibility therefor and deal with the matter on its own.
(9) The user shall submit the Strain Receipt Form within one month of the date of the receipt.
The user may request that the strains be sent again without charge if the strain does not show
good growth during this warranty period.

The NIES-Collection shall not bear any

responsibility for mistakes by the user.

2. Order procedures(1) All requests to the NIES-Collection for strains shall be by completing the “Strain Ordering andAgreement Form” (p.38), and by sending it via mail to the following address:
Microbial Culture Collection
National Institute for Environmental Studies,- 35 -

16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Phone : +81-29-850-2556
Fax : +81-29-850-2587
Email: mcc@nies.go.jp
Please note that we can ship the strain only after we accept the original copy (with theuser’s signature and date) of the order form.
(2) Upon receipt of a strain, the “Strain Receipt Form” (p.44) should be completed and returned to
the NIES-Collection within one month.

3. Price of strains

Sales tax (5%) and postage will be requested in addition to the culture price for 15 mL.
Academic purpose

6,000 yen

Commercial purpose

10,000 yen

For educational use in schools and universities, the NIES-Collection distributes selectedstrains without charge.

Please contact the staff of the NIES-Collection. In this case, the user cannot

specify species and strains.

4. Special warning for distribution of "Untransportable" and "[Cryopreserved]" strains

Orders for the strains designated as "Untransportable" in the strain description may not be
accepted, depending on the season or condition of the cultures. For transport overseas, such strains
should in principle be carried personally (e.g., in hand luggage). Such transportation shall bearranged by individual requestors.
For strains designated as "[Cryopreserved]" in the strain description, frozen cells are thawed
and inoculated into fresh medium just after the order is accepted.
month for the overseas shipping of these strains.

As a result, it takes at least one

See also Appendix I.

5. Special warning for distribution of strains collected outside of Japan since 1993

Distribution of some strains collected and isolated outside of Japan since 1993 is now
suspended pursuant to the Convention of Biological Diversity, although the strain data are available
in the catalogue.

Please contact the staff of the NIES-Collection if you wish to request those strains.

- 36 -

6. Special warning for “Toxic” strains

Strains that have been reported to produce toxic substance are shown “toxic” in theirdescriptions (See also Appendix II). Users who order those strains must complete the “Toxic Strain
Ordering and Agreement Form” (p.39) and send it to the NIES-Collection.

7. Distribution to researchers belonging to the NIES

Strains are available without charge.

Please complete the “Strain Ordering and Agreement

Form” (pp.40〜43) and send it via in-house mail or pass it to the staff directly.

- 37 -

Strain Ordering and Agreement Form
Date:
Requestor’s full name (family name with capital letters):
Requestor’s affiliation and address:
Tel:
Fax:
E-mail:I request the following culture strain(s).
Scientific name(s) and strain number(s):Object of use (in detail):

Do you need the strain data? (Yes or No):I accept the following conditions for ordering the strain(s).
Signature

Printed name

Date

Agreement for distribution
1. The strains (including replicates and derivatives from the strains), which are distributed from the
NIES-Collection, shall be available for education, research, and development purpose only. The
strains are not intended to apply directly to humans. The user hereby acknowledges and accepts thepotential risks of the strains and shall use them in compliance with domestic and foreign laws,
regulation, and guidelines.
2. The user shall be requested to submit the application form personally.
3. The user shall not acquire any intellectual property rights by the purchase of the strain.
4. The user shall provide written notice to the NIES when the purpose has changed considerably fromthe purpose that was stated at the time of submission.
5. The user shall not distribute the strains, replicates and derivatives to any third party.
6. The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or photocopies
thereof to the NIES-Collection.
7. When the use of the strain violates another person’s rights, the user shall bear responsibility therefor,and deal with the matter on its own.
8. The user shall acknowledge the possibility that the strain is deficient and harmful, and inadequate to
the user’s aim. Thus, if the user suffers any loss by the strain, he/she shall bear responsibility
therefor and deal with the matter on its own.
9. The user shall submit the Strain Receipt Form within one month of the date of the receipt. The user
may request that the strains be sent again without charge if the strain does not show good growthduring this warranty period. The NIES-Collection shall not bear any responsibility for mistakes by
the user.

- 38 -

Toxic Strain Ordering and Agreement Form
Date:
Requestor’s full name (family name with capital letters):
Requestor’s affiliation and address:
Tel:
Fax:
E-mail:I request the following culture strain(s).
Scientific name(s) and strain number(s):Object of use (in detail):

Do you need the strain data? (Yes or No):I accept the following conditions for ordering the strain(s).
Signature

Printed nameSignature of responsible person

Printed name

Date
(Please sign if the requestor is a student/ part-time staff.)

Date

Agreement for distribution
1. The strains (including replicates and derivatives from the strains), which are distributed from the
NIES-Collection, shall be available for education, research, and development purpose only. The
strains are not intended to apply directly to humans. The user hereby acknowledges and accepts therisks of toxic strains and shall use them in compliance with domestic and foreign laws, regulation,and guidelines. The user shall store and discard them appropriately.
2. The user shall be requested to submit the application form personally.
3. The user shall not acquire any intellectual property rights by the purchase of the strain.
4. The user shall provide written notice to the NIES when the purpose has changed considerably fromthe purpose that was stated at the time of submission.
5. The user shall not distribute the strains, replicates and derivatives to any third party.
6. The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or photocopies
thereof to the NIES-Collection.
7. When the use of the strain violates another person’s rights, the user shall bear responsibility therefor,and deal with the matter on its own.
8. The user shall acknowledge the possibility that the strain is deficient and harmful, and inadequate to
the user’s aim. Thus, if the user suffers any loss by the strain, he/she shall bear responsibility
therefor and deal with the matter on its own.
9. The user shall submit the Strain Receipt Form within one month of the date of the receipt. The user
may request that the strains be sent again without charge if the strain does not show good growthduring this warranty period. The NIES-Collection shall not bear any responsibility for mistakes by
the user.

- 39 -

Strain Ordering and Agreement Form (For NIES staff)
Date:
Requestor’s full name (family name with capital letters):
Requestor’s affiliation at NIES:
Tel:
Fax:
E-mail:I request the following culture strain(s).
Scientific name(s) and strain number(s):Object of use (in detail):

Registered research name:Registered research code:
Do you need the strain data? (Yes or No):I accept the following conditions for ordering the strain(s).
Signature

Printed name

Date

Agreement for distribution
1. The strains (including replicates and derivatives from the strains), which are distributed from the
NIES-Collection, shall be available for education, research, and development purpose only. The
strains are not intended to apply directly to humans. The user hereby acknowledges and accepts thepotential risks of the strains and shall use them in compliance with domestic and foreign laws,
regulation, and guidelines.
2. The user shall be requested to submit the application form personally.
3. The user shall not acquire any intellectual property rights by the purchase of the strain.
4. The user shall provide written notice to the NIES when the purpose has changed considerably fromthe purpose that was stated at the time of submission.
5. The user shall not distribute the strains, replicates and derivatives to any third party.
6. The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or photocopies
thereof to the NIES-Collection.
7. When the use of the strain violates another person’s rights, the user shall bear responsibility therefor,and deal with the matter on its own.
8. The user shall acknowledge the possibility that the strain is deficient and harmful, and inadequate to
the user’s aim. Thus, if the user suffers any loss by the strain, he/she shall bear responsibility
therefor, and deal with the matter on its own.
9. The user shall submit the Strain Receipt Form within one month of the date of the receipt. The user
may request that the strains be sent again without charge if the strain does not show good growthduring this warranty period. The NIES-Collection shall not bear any responsibility for mistakes by
the user.

- 40 -

Toxic Strain Ordering and Agreement Form (For NIES staff)
Date:
Requestor’s full name (family name with capital letters):
Requestor’s affiliation and address:
Tel:
Fax:
E-mail:I request the following culture strain(s).
Scientific name(s) and strain number(s):Object of use (in detail):
Registered research name:Registered research code:
Do you need the strain data? (Yes or No):I accept the following conditions for ordering the strain(s).
Signature

Printed nameSignature of responsible person

Printed name

Date
(Please sign if the requestor is a student/ part-time staff.)

Date

Agreement for distribution
1. The strains (including replicates and derivatives from the strains), which are distributed from the
NIES-Collection, shall be available for education, research and development purpose only. The
strains are not intended to apply directly to humans. The user hereby acknowledges and accepts therisks of toxic strains and shall use them in compliance with domestic and foreign laws, regulations
and guidelines. The user shall store and discard them appropriately.
2. The user shall be requested to submit the application form by personally.
3. The user shall not acquire any intellectual property rights by the purchase of the strain.
4. The user shall provide written notice to the NIES when the purpose has changed considerably fromthe purpose that was stated at the time of submission.
5. The user shall not distribute the strains, replicates and derivatives to any third party.
6. The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or photocopies
thereof to the NIES-Collection.
7. When the use of the strain violates another person’s rights, the user shall bear responsibility therefor,and deal with the matter on its own.
8. The user shall acknowledge the possibility that the strain is deficient and harmful, and inadequate to
the user’s aim. Thus, if the user suffers any loss by the strain, he/she shall bear responsibility
therefor and deal with the matter on its own.
9. The user shall submit the Strain Receipt Form within one month of the date of the receipt. The user
may request that the strains be sent again without charge if the strain does not show good growthduring this warranty period. The NIES-Collection shall not bear any responsibility for mistakes by
the user.

- 41 -

Strain Ordering and Agreement Form (For guest researchers and collaborators)
Date:Requestor’s full name (family name with capital letters):
Requestor’s affiliation and address:
Tel:
Fax:
E-mail:Responsible researcher at NIES
Affiliation
I request the following culture strain(s).
Scientific name(s) and strain number(s):Object of use (in detail):

Do you need the strain data? (Yes or No):I accept the following conditions for ordering the strain(s).
Signature

Printed name

Date

Agreement for distribution
1. The strains (including replicates and derivatives from the strains), which are distributed from the
NIES-Collection, shall be available for education, research and development purpose only. The
strains are not intended to apply directly to humans. The user hereby acknowledges and accepts thepotential risks of the strains and shall use them in compliance with domestic and foreign laws,
regulations and guidelines.
2. The user shall be requested to submit the application form by personally.
3. The user shall not acquire any intellectual property rights by the purchase of the strain.
4. The user shall provide written notice to the NIES when the purpose has changed considerably fromthe purpose that was stated at the time of submission.
5. The user shall not distribute the strains, replicates and derivatives to any third party.
6. The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or photocopies
thereof to the NIES-Collection.
7. When the use of the strain violates another person’s rights, the user shall bear responsibility therefor,and deal with the matter on its own.
8. The user shall acknowledge the possibility that the strain is deficient and harmful, and inadequate to
the user’s aim. Thus, if the user suffers any loss by the strain, he/she shall bear responsibility
therefor and deal with the matter on its own.
9. The user shall submit the Strain Receipt Form within one month of the date of the receipt. The user
may request that the strains be sent again without charge if the strain does not show good growthduring this warranty period. The NIES-Collection shall not bear any responsibility for mistakes by
the user.

- 42 -

Toxic Strain Ordering and Agreement Form (For guest researchers and collaborators)
Date:Requestor’s full name (family name with capital letters):
Requestor’s affiliation and address:
Tel:
Fax:
E-mail:I request the following culture strain(s).
Scientific name(s) and strain number(s):Object of use (in detail):
Do you need the strain data? (Yes or No):I accept the following conditions for ordering the strain(s).
Signature

Printed nameSignature of responsible person

Printed name

Date
(Please sign if the requestor is a student/ part-time staff.)

Date

Agreement for distribution
1. The strains (including replicates and derivatives from the strains), which are distributed from the
NIES-Collection, shall be available for education, research, and development purpose only. The
strains are not intended to apply directly to humans. The user hereby acknowledges and accepts therisks of toxic strains and shall use them in compliance with domestic and foreign laws, regulation,and guidelines. The user shall store and discard them appropriately.
2. The user shall be requested to submit the application form personally.
3. The user shall not acquire any intellectual property rights by the purchase of the strain.
4. The user shall provide written notice to the NIES when the purpose has changed considerably fromthe purpose that was stated at the time of submission.
5. The user shall not distribute the strains, replicates and derivatives to any third party.
6. The user shall use the NIES strain number (e.g., NIES-125) when he/she uses a NIES strain in apaper which is subsequently published, and shall send two copies of the reprint(s) or photocopies
thereof to the NIES-Collection.
7. When the use of the strain violates another person’s rights, the user shall bear responsibility therefor,and deal with the matter on its own.
8. The user shall acknowledge the possibility that the strain is deficient and harmful, and inadequate to
the user’s aim. Thus, if the user suffers any loss by the strain, he/she shall bear responsibility
therefor and deal with the matter on its own.
9. The user shall submit the Strain Receipt Form within one month of the date of the receipt. The user
may request that the strains be sent again without charge if the strain does not show good growthduring this warranty period. The NIES-Collection shall not bear any responsibility for mistakes by
the user.

- 43 -

Strain Receipt Form
Date:
Recipient’s full name (family name with capital letters):
Recipient’s affiliation and address:

Tel:
Fax:
E-mail:I received the following culture strain(s).
Date of strain receipt:
Scientific name(s) and strain number(s):States of strain(s) received:□Good (strain number)□Poor (strain number)□Others (strain number)Comments:- 44 -

IV. ESTABLISHMENT OF FRESH CULTURES
When investigators are to receive culture strains, the following steps should be carried out toestablish fresh cultures.

i)Appropriate culture media should be prepared before receipt of the strains according to therecipes given in Chap. VI and with reference to the basic methods given in Chap. V.

ii)

Immediately after receipt, cultures should be unpacked, transferred to new media and grownat the temperature and light intensity directed by the Collection (cf. Chap. VIII); the light-darkcycle should be 12 hours light : 12 hours dark, and the screw-cap on the tube should be loosened.

iii)

After detecting good growth, further maintenance of cultures requires transfer into new media
at intervals suggested by the Collection (cf. Chap. VIII).

V. BASIC METHODS FOR PREPARATION OF ALGAL CULTURE MEDIA
A number of media are used for maintenance of algal cultures and prepared according to therecipes given in the next chapter. The present chapter introduces the basic methods for preparation
adopted in the Global Environmental Forum.

1.

Stock solutions

Media are generally composed of three components, macronutrients, trace metals andvitamins (cf. Table 2) and prepared from stock solutions of these components. The concentration ofstock solutions of trace metals and vitamins is very low and primary stock solutions are prepared for
dilution to obtain the stock solutions.
A.

Macronutrients : Separate stock solutions with a concentration of 10 mg/ml of eachmacronutrient are prepared and stored in a refrigerator (5°C).
B.

Trace metals : These elements are prepared either as separate stock solutions or mixed stock

solutions.
(1)

Separate stock solutionsi) Prepare a separate primary solution with a concentration of 10-100 mg/ml.
ii) Dilute each primary solution to prepare the stock solution with a concentration of 1 mg/ml
and store in a refrigerator (5°C).

- 45 -

Table 2. Chemicals employed for culture media______________________________________________________________________________
Macronutrients
Trace metals
NaCl
H3BO3
KCl
MnCl2·4H2O
CaCl2·2H2O
MnSO4·7H2O
MgCl2·6H2O
FeCl3·6H2O
Na2SO4FeSO4·7H2O
K2SO4
CoCl2·6H2O
MgSO4·7H2O
ZnSO4·7H2O
NaNO3
CuSO4·5H2O
KNO3Na2MoO4·2H2O
Ca(NO3)2·4H2O
Vitamins
NH4NO3
Vitamin B12
NaH2PO4·2H2O
Biotinβ-Na2 glycerophosphate·5H2O
Thiamine HCl
KH2PO4
Nicotinic acidK2HPO4
Calcium panthothenate
Na2CO3
p-Aminobenzoic acid
NaHCO3
Inositol
Na2SiO3·9H2O
Folic acid
Thymine______________________________________________________________________(2)

Mixed stock solutioni) Same as (1)-i)ii) Add approximately 80 % of the required volume of distilled water to a beaker.
iii) Dissolve the required amount of Na2EDTA, while stirring continuously.
iv) Dispense the required volume of each trace metal from primary solution, while stirringcontinuously.
v) Dilute to final volume with distilled water and store in a refrigerator (5°C).

C.

Vitamins : Only three vitamins, vitamin B12, biotin, and thiamine HCl have been foundnecessary for growth of many microalgae and are added to most media. Some media, in addition,
contain other vitamins(1)

Vitamin B12, biotin and thiamine HCli) Prepare separate primary stock solution with a concentration of 0.1 mg/ml of vitamin B12and biotin and 10 mg/ml of thiamine HCl.
ii) After dispersing 1 ml of these primary stock solution into each of a number of test tubes
and autoclaving (121°C, 20 min), store in a freezer at −20°C.
iii) Thaw and dilute 1 ml of primary stock solution of each vitamins to prepare the working
stock solution with a concentration of 1 µg/ml of vitamin B12 and biotin or of 100 µg/ml
of thiamine HCl, and store in a refrigerator (5°C).

(2)

Other vitamins: Additional vitamins are added to some media in the forms of mixes (cf. Chap.

VI-64). It is recommended to prepare a large volume of mixed stock solution.

- 46 -

i) Prepare a separate primary solution with a concentration of 0.1-1.0 mg/ml.
ii) Add approximately 80 % of the required volume of distilled water to a beaker.
iii) Dispense the required volume of each vitamin from the primary solution, while stirringcontinuously.
iv) After sterilization by passing through a Millipore filter (0.22 µm), aseptically dispense
100 ml of the mixed stock solution into each of a number of vessels and store in a freezer
at −20°C.

2. MediaMedia are divided broadly into two categories, synthetic and enriched. The former are used formaintenance of all freshwater algal cultures and some marine ones and the latter for most marine ones.
(1)

Synthetic medium (freshwater)i) Add approximately 80-90% of the required volume of distilled water to a beaker.
ii) Dissolve appropriate quantities of weighed buffer such as Tris (hydroxymethyl)aminomethane (known as Tris), glycylglycine, HEPES, TAPS, Bicine, MES or 1, 2, 3, 4cyclopentan tetracarboxylic acid (if required), while stirring continuously. These buffers
are easily soluble with stirring.
iii) Dispense the appropriate nutrients from previously prepared stock solutions, while stirringcontinuously.
iv) Dilute to final volume with distilled water.
v) Check the pH and make any adjustments with either 1N HCl or 1N NaOH (if buffers
required) or with either 0.1N HCl or 0.1N NaOH (if no buffers required).
vi) Dispense 10 ml of medium into each of the test tube (18×150mm) and sterilize by
autoclaving (121°C, 20 min).

(2)

Synthetic medium (marine)i) Add approximately 80% of the required volume of distilled water to a beaker.
ii) Dissolve appropriate quantities of weighed Tris, Nitrilotriacetic acid (known as NTA) andmajor salts such as NaCl, MgSO4·7H2O, KCl and CaCl2·2H2O, while stirringcontinuously.
iii) Dispense the other nutrients from previously prepared stock solutions.
iv) Dilute to the final volume with the distilled water.
v) Check the pH, which is usually adjusted to 8.0 with 1N HCl.
vi) Dispense 10 ml of medium into each of the test tubes and sterilize by autoclaving (121°C,
20 min).

- 47 -

(3)Enriched seawater mediumi) Collect offshore water free from gross pollution and remove particulate matter with
Whatman GF/C filters.
ii) Check the salinity. A salinity of 35‰ is considered normal seawater.
iii) Add approximately 80-90% of the required volume of seawater to a beaker.
iv) Dissolve appropriate quantities of weighed Tris (if required).
v) Dispense the appropriate nutrients from previously prepared stock solutions.
vi) Dilute to the final volume with seawater.
vii) Check the pH and adjust to 8.0 with 1N HCl if necessary.
viii) Dispense 10 ml of medium into each test tube and sterilize by autoclaving (121°C,
20 min).

3. Agar slant

Agar is added usually at concentrations of 1.5% after liquid medium has been prepared, prior to
autoclaving.
i) Add the appropriate quantities of weighed agar to liquid medium and heat to 121°C by
autoclaving to melt all the agar.
ii) After melting, quickly dispense 10 ml of agar medium into each test tube and sterilize by
autoclaving (121°C, 20 min).
iii) After sterilization, lay the upper part of the test-tube on a rod (1 cmφ) and cool to form
an agar slant.

- 48 -

VI. MEDIA

1)1 )-1.

(163)１）NaNO３NH４NO３
MgSO４･7H２O
KH２PO４K２HPO４
CaCl２･2H２O
CaCO３2)Fe-citrate
Citric acid
BiotinThiamine HCl
Vitamin B６
Vitamin B１２
Trace metals２）
Distilled water
pH 6.6３）

3)

2.

14
2.2
3
1
0.5
1
1
0.2
0.2
0.2
1
0.1
0.1
0.5
99.5

Allen

(1)

(NH４)２SO４
KH２PO４MgSO４･7H２O
CaCl２･2H２O
Allen Metals１）
Distilled water
pH 2.5２）

For freshwater algae

1. AF-6

1)
2)

3.

Stock media for algae

mg
mg
mg
mg
mg
mg
mg
mg
mg
µg
µg
µg
µg
ml
ml

1)
2)

(12)

NaNO３
KH２PO４K２HPO４MgSO４･7H２O
CaCl２･2H２O
NaCl
KOH
FeSO４･7H２O
H３BO３
ZnSO４･7H２O
MnCl２･7H２O
MoO３
CuSO４･5H２O
Co(NO３)２･6H２O
Na２EDTA
Distilled waterAF-6 / 2

AF-6 medium is diluted with distilled water to 1 / 2.

- 49 -

mg
mg
mg
mg
ml
ml

See 55
pH is adjusted to 2.5 with 1 N H２SO４.

4. BBM

Reference number in parentheses.
In the NIES-Collection, CaCO ３ is removed andPIV metals are used instead of trace metals.
pH is adjusted to 6.6 by buffering with 40 mg MES
in the NIES-Collection.

132
27.2
24.6
7.4
0.01
99.9

25
mg
17.5
mg
10
mg
7.5
mg
2.5
mg
2.5
mg
3.1
mg
0.498 mg1.142 mg
0.882 mg0.144 mg
0.071 mg0.157 mg
0.049 mg
5
mg
100
ml

5. C (90)
Ca(NO３)２･4H２O
KNO３β-Na２glycerophosphate･5H２O
MgSO４･7H２O
Vitamin B１２
BiotinThiamine HCl
PIV metals１）
Tris (hydroxymethyl)aminomathane
Distilled water
pH 7.5
1)

8.
15
10
5
4
0.01
0.01
1
0.3

mg
mg
mg
mg
µg
µg
µg
ml

50
99.7

mg
ml

Carefoot

NaNO３
CaCl２･2H２O
MgSO４･7H２O
K２HPO４
KH２PO４NaCl
PIV metals1)
Distilled water
pH 7.5

(16)
24.7
1.1
4.7
0.9
2.3
1.5
0.5
99.5

mg
mg
mg
mg
mg
mg
ml
ml

* In the NIES-Collection, 0.02 µg Vitamin B１２, 0.02 µg
Biotin and 2 µg Thiamine HCl are added to this medium.
1) See 62

See 62

9. CB
6. CA (102)
Ca(NO３)２･4H２O
KNO３
NH４NO３β-Na２glycerophosphate･5H２O
MgSO４･7H２O
Vitamin B１２
BiotinThiamine HCl
PIV metals１）
Fe (as EDTA; 1:1 molar)２）
HEPES
Distilled water
pH 7.2
1)
2)

2
10
5
3
2
0.01
0.01
1
0.1
0.1
40
99.9

C medium with pH adjusted to 9.0 by buffering withBicine instead of Tris (hydroxymethyl) aminomethane.

mg
mg
mg
mg
mg
µg
µg
µg
ml
mg
mg
ml

10. CC (96)
C medium with pH adjusted to 3.0 by buffering with 1, 2,
3, 4 - cyclopentan tetracarboxylic acid instead of Tris
(hydroxymethyl) aminomethane.

11.

See 62
See 58CSi

C medium with pH adjusted to 7.0 by buffering with 50
mg HEPES instead of Tris (hydroxymethyl) aminomethane. Thereafter, 10 mg Na２SiO３･9H２O is added.

7. CAM
12.
CA medium with pH adjusted to 6.5 by buffering with
MES instead of HEPES.

CSi+Cu0.25 mg CuSO４･5H２O is added to CSi medium.

- 50 -

13. CT (522)18.

C medium with pH adjusted to 8.2 by buffering with 40
mg TAPS instead of Tris (hydroxymethyl) aminomethane.

KH２PO４MgSO４･7H２O
Sodium acetate
Potassium citrate
Polypeptone
Yeast extract
Vitamin B１２Thiamine HClDistilled water
pH 6.4

14. CYT
10 mg Yeast extract and 20 mg Tryptone are added to C
medium.

HUT

(89)
2
2.5
40
4
60
40
0.05
0.04
100

mg
mg
mg
mg
mg
mg
µg
mg
ml

* Add 150 mg agar to 100 ml of the medium for semi-

15.

C+10%Seawater (N. Tezuka, unpubl.)

solid medium.

C medium with 10% filtrated seawater.

19.
16. D

(17)

Ca(SO４)･2H２O
KNO３
NaNO３
Na２HPO４NaCl
MgSO４･7H２O
FeCl３･6H２O
NTA
D solution１）
Distilled water

6
10.3
68.9
11.1
0.8
10
0.05
10
0.05
100

M-11

NaNO３
K２HPO４MgSO４･7H２O
CaCl２･2H２O
Na２CO３
FeSO４･7H２O
Na２EDTA･2H２O
Distilled water
pH 8.0

mg
mg
mg
mg
mg
mg
mg
mg
ml
ml

20. MA1)17.

(60), (553)
10
1
7.5
4
3
0.1
0.1
100

mg
mg
mg
mg
mg
mg
mg
ml

5
10
5
4
5
10
0.5
0.05
0.5
0.05
0.5
0.08
2
50
100

mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
ml

(92)

See 57Ca(NO３)２･4H２O
KNO３
NaNO３
Na２SO４MgCl２･6H２O
β-Na２glycerophosphate･5H２O
Na２EDTA
FeCl３･6H２O
MnCl２･4H２O
ZnCl２
CoCl２･6H２O
Na２MoO４･2H２O
H３BO３
BicineDistilled water
pH 8.6

DH+Fe (I.I. Brown, unpubl.)

D medium with 1.14 mg FeCl２･6H２O and adjusted to pH
8.24-8.26 by buffering with 120mg HAPES.

- 51 -

21.

MAF-6

24.

M Chu No. 10

Ca(NO３)２･4H２O
KH２PO４MgSO４･7H２O
Na２CO３
Na２SiO３･9H２O
HCl (1N)１）Na２EDTA･2H２O
FeCl３･6H２O
H３BO３
MnCl２･4H２O(NH４)６Mo７O２４･4H２O
Vitamin B１２Thiamine HCl
BiotinDistilled water
1)

(20)
2.0
mg
0.62 mg
2.5
mg
2
mg
2.5
mg
0.025 ml
0.2
mg
0.1
mg
0.248 mg0.139 mg
0.1
mg
1
µg
0.1
µg
0.1
µg
100
ml

1)
2)

mg
mg
mg
mg
µg
µg
µg
ml
ml
mg
ml

See 62
See 58MG medium with pH adjusted to 6.5 by buffering with
MES instead of HEPES.

In the NIES-Collection, pH is adjusted to 7.6 withrespective volume of 1 N HCl.

100
25
25
10
1
0.1
0.1
1.5
99.8

MW

(397)

Urea
NaNO３
NH４Cl
Ca(NO３)２･4H２O
CaCO３
CaCl２･2H２O
KNO３
KHCO３
β-Na２glycerophosphate･5H２O
MgSO４･7H２O
PIV metals１）
Vitamin B１２Thiamine HCl
BiotinGlycylglycine
Distilled water
pH 7.2

MDM (491)

KNO３
MgSO４･7H２O
K２HPO４NaCl
CaCl２･2H２O
Fe solution１）
A５ solution２）
Agar
Distilled water
pH 8.0
1)
2)

2
10
3
2
0.01
0.01
1
0.1
0.1
40
99.9

25. MGM

26.
23.

(91)

Ca(NO３)２･4H２O
KNO３β-Na２glycerophosphate･5H２O
MgSO４･7H２O
Vitamin B１２
BiotinThiamine HCl
PIV metals１）
Fe (as EDTA; 1:1 molar)２）
HEPES
Distilled water
pH 7.210 mg glucose and 10 mg sodium acetate are added toAF-6 medium.

22.

MG

mg
mg
mg
mg
mg
ml
ml
g
ml

See 59See 541)- 52 -

See 620.85 mg
0.17 mg
0.042 mg
10
mg
1
mg
1.4
mg
1
mg
0.9
mg
2
mg
1.5
mg
0.05 ml
0.02 µg2µg0.02 µg10mg99.95 ml27.

30. VT (391)

MW / 5Ca(NO３)２･4H２O
β-Na２glycerophosphate･5H２O
MgSO４･7H２O
KCl
Vitamin B１２
BiotinThiamine HCl
PIV metals１）
Glycylglycine
Distilled water
pH 7.5

MW medium is diluted with distilled water to 1 / 5.

28. P 35

(92)

NH４NO３
MgSO４･7H２O
KCl
CaCl２･2H２O
β-Na２glycerophosphate･5H２O
Sodium acetate
Vitamin B１２
BiotinThiamine HCl
PIV metals１）
Tris (hydroxymethyl)aminomethane
Distilled water
pH 8.0
1)

10
4
5
7.4
5
100
0.01
0.01
1
0.3

mg
mg
mg
mg
mg
mg
µg
µg
µg
ml

1)

31.
50
99.7

mg
ml

See 62VTAC (332)

See 62

URO

VTYT

(96)

10 mg yeast extract and 20 mg tryptone are added to VT
medium.

(177), (263)

NH４NO３β-Na２glycerophosphate･5H２O
MgSO４･7H２O
CaCl２･2H２O
KCl
Thiamine HCl
Vitamin B１２
BiotinFe-EDTAPIV metals１）
Distilled water
pH 7.52)
1)
2)

mg
mg
mg
mg
µg
µg
µg
ml
mg
ml

20 mg sodium acetate is added to VT medium.

32.
29.

11.78
5
4
5
0.01
0.01
1
0.3
50
99.7

0.5
0.4
1
1
0.1
1
0.01
0.01
0.05
0.1
99.9

mg
mg
mg
mg
mg
µg
µg
µg
mg
ml
ml

33.

W

(519)

Ca(NO３)２･4H２O
KNO３
MgSO４･7H２O
β-Na２glycerophosphate･5H２O
Urea
Thiamine HCl
Vitamin B１２
Biotin
PIV metals１）
Glycylglycine
Distilled water
pH 7.5

See 62
pH is adjusted to 7. 5 with 0.1 N HCl.

1) See 62- 53 -

10
mg
1
mg
1.5
mg
2
mg
1.7
mg
0.2
µg
0.002 µg0.002 µg0.05 ml
10mg
99.95 ml

34. SW(386)

1 )-2.

A small amount of dried soil is put into a test tube, and
20 ml distilled water is added.

35. SOT

1.68
50
250
100
100
20
4
1
8
0.1
99.9

ESM

(356)

NaNO３
K２HPO４
Vitamin B１２
BiotinThiamine HCl
Fe-EDTA
Mn-EDTA
Tris (hydroxymethyl)aminomethane
Soil extract１）
Sea water
pH 8.0(349)

NaHCO３K２HPO４
NaNO３
K２SO４
NaCl
MgSO４･7H２O
CaCl２･2H２O
FeSO４･7H２O
Na２EDTAA５ solution１）
Distilled water1)

36.

For marine algae

g
mg
mg
mg
mg
mg
mg
mg
mg
ml
ml

1)

12
0.5
0.1
0.1
10
25.9
33.2

mg
mg
µg
µg
µg
µg
µg

100
5
95

mg
ml
ml

7.5
0.6
0.05
0.05
10
1
0.1
99.9

mg
mg
µg
µg
µg
mg
ml
ml

See 65See 54

37.

f/2

(59)

NaNO３
NaH２PO４･2H２O
Vitamin B１２
BiotinThiamine HCl
Na２SiO３･9H２O
f / 2 metals１）
Sea water
1)- 54 -

See 6038. M-ASP7 (541)
NaCl
MgSO４･7H２O
KCl
CaCl２･2H２O
NaNO３
NaH２PO４･2H２O
Vitamin B１２
Vitamin mix S３１）
Na２SiO３･9H２O
PＮ metals２）
Tris (hydroxymethyl)aminomethane
NTA
Distilled water
pH 8.0
1)
2)

41.
2.5
900
70
30
5
2
0.1
1
1
3

g
mg
mg
mg
mg
mg
µg
ml
mg
ml

100
7
96

mg
mg
ml

MNK (297)

See 64
See 63

42. URO-YT
39.

MF

43. URO-1/10YT

See 6540. MKM
KNO３
KH２PO４MgSO４･7H２O
Fe-citrate
Agar
Sea water
Distilled water(246)

10 mg yeast extract and 20 mg tryptone are added to URO
medium, which is made with seawater instead of distilledwater.

f / 2 medium with Na２SiO３･9H２O replaced by 1.0ml
soil extract1) and adjusted to pH 8.0 by buffering with
100mg Tris (hydroxymethyl) aminomethane.
1)

2
mg
0.1
mg
0.028 mg
0.015 µg0.015 µg2
µg0.12 µg0.24 µg0.9µg0.006 µg0.003 µg0.07 µg0.37 µg
2.6
µg
3.3
µg
100
ml

NaNO３
K２HPO４
Na２HPO４･12H２O
Vitamin B１２
BiotinThiamine HCl
CoSO４･7H２O
ZnSO４･7H２O
MnCl２･4H２O
CuSO４･5H２O
Na２SeO３
Na２MoO４･2H２O
Na２EDTA･2H２O
Fe-EDTA
Mn-EDTASea water(247)

1 mg yeast extract and 2 mg tryptone are added to URO
medium, which is made with seawater instead of distilledwater.

(491)
75
2.5
2
250
1.5
50
50

mg
mg
mg
µg
g
ml
ml

44.

WESM

ESM medium with 95 ml sea water replaced by 85 ml sea
water and 10 ml distilled water.

- 55 -

2)

Bacteria-free check media

2 )-1.

2 )-2.

51. STPFor freshwater algae

46. B - I

ml
mg
mg

100
100

ml
mg

(103)

Stock medium
Proteose peptone

47.

100
100
200

(389)

NaNO３
K２HPO４
Sodium glutamate
GlucoseGlycineD, L - Alanine
Vitamin mix 8１）
Trypticase
Yeast autolysate２）
SucroseSoil extract３）
Sea water
Distilled water
pH 7.5

45. YT (96)Stock medium
Yeast extract
Tryptone

For marine algae

20
1
50
20
10
10
0.1
20
20
100
5
80
15

mg
mg
mg
mg
mg
mg
ml
mg
mg
mg
ml
ml
ml

B - II (103)1)

Stock medium
Yeast extract

48.

100
500

ml
mg

In the NIES-Collection, vitamin mix 8 is replaced byVitamin mix S３.
2) In the NIES-Collection, yeast autolysate is replacedby yeast extract.
3) See 65

100
500
300

ml
mg
mg

52.

B - III (103)

Stock medium
PeptoneBeef extract

50.

B-V

Stock medium
Sodium acetate
GlucoseTryptone
Yeast extract

100
100
100

ml
mg
mg

100
50
50
50
30

ml
mg
mg
mg
mg

( M. Tatewaki, pers. comm.)

NaCl
MgSO４･7H２O
KCl
NaNO３
CaCl２･2H２O
K２HPO４
SucrosePII metals１）
FeCl３･6H２O
Thiamine HCl
BiotinVitamin B１２
C-Source Mix II２）
Tris (hydroxymethyl)aminomethane
Distilled water
pH 8.049. B - IV (103)
Stock medium
GlucosePeptone

MM23

(103)

1)
2)

- 56 -

See 61
See 56

1.8
500
60
100
36.7
6
400
2
48
10
0.1
0.2
1

g
mg
mg
mg
mg
mg
mg
ml
µg
µg
µg
µg
ml

100
97

mg
ml

53.

Bf / 2 (573)

56.

ASP7１）
Trypticase
Yeast extract
1)

100
50
5

ml
mg
mg

GlycineD, L - Alanine
L - Aspargine
Sodium acetate･3H２O
GlucoseL - Glutamic acid
Distilled water

In the NIES-Collection, ASP7 is replaced by f / 2
medium.

57.

3 ) Trace metals, vitamin mixes andsoil extract
54.

A5 solution

55.

Allen metals１）

Fe-EDTA
MnCl２･4H２O
H３BO３
ZnSO４･7H２O
CuSO４･5H２O(NH４)６MoO２４･ 4H２O
NH４VO３Distilled water

286
250
22.2
7.9
2.1
100

mg
mg
mg
mg
mg
ml

(1)
30.16
1.79
2.86
220
79
130
23
100

ml
mg
mg
mg
mg
mg
mg
ml

Fe (as EDTA; 1:1 molar) (388)
70.2
66
100

mg
mg
ml

1 ml of this solution contains 0.1 mg Fe.

Fe solution(96)

FeSO４･7H２O
Distilled water
Conc･H２SO４
1) 2 drops / 500ml (Ref. 96).

- 57 -

0.05
228
50
50
2.5
2.5
4.5
99.95

58.

*

In the NIES-Collection, diluted with distilled water to
1 / 1,000.

mg
mg
mg
mg
mg
mg
ml

Indicated as “Micronutrient solution” in Ref.17.

59.
1)

100
100
100
200
200
200
100

1)

Fe(NH４)２(SO４)２･6H２O
Na２EDTA･2H２O
Distilled water

g
g
g
mg
mg
mg
mg
ml

(M. Tatewaki, pers. comm.)

D solution１） (17)Conc･H２SO４
MnSO４･H２O
ZnSO４･7H２O
H３BO３
CuSO４･5H２O
Na２MoO４･2H２O
CoCl２･6H２O
Distilled water

(85)

H３BO３
MnSO４･7H２O
ZnSO４･7H２O
CuSO４･5H２O
Na２MoO４･2H２O
Distilled waterC - Source Mix II

200
mg
100
ml
0.026 ml1)

60.

f / 2 metals (59)Na２EDTA･2H２O
FeCl３･6H２O
CoSO４･7H２O
ZnSO４･7H２O
MnCl２･4H２O
CuSO４･5H２O
Na２MoO４･2H２O
Distilled water

61. P II metals

64.
440
316
1.2
2.1
18
0.7
0.7
100

mg
mg
mg
mg
mg
mg
mg
ml

114
4.9
16.4
2.2
480
100
100

mg
mg
mg
mg
µg
mg
ml

Thiamine HCl
Nicotinic acid
Calcium pantothenate
p - Aminobenzoic acid
BiotinInositolFolic acid
ThymineDistilled water

65.

19.6
3.6
2.2
0.4
0.25
100
100

Na２EDTA･2H２O
H３BO３
FeCl３･6H２O
CoSO４･7H２O
ZnSO４･7H２O
MnCl２･4H２O
Distilled water

Stock medium for protozoa

66. LE

mg
mg
mg
mg
mg
mg
ml

L Solution: White part of lettuce is dried at 90 °C for
16 - 18 h without scorching. 300 mg of the dried lettuce isadded to 100 ml boiling water (9 : 1 distilled water / tapwater) and boiled for 30 minutes, while stirring. The
supernatant is passed through cottonwool.
E solution: 300 mg of crushed yolk of hardboiled egg
is added to 100ml water (9 : 1 distilled water / tap water)and boiled for 30 minutes, while stirring. The supernatantis passed through cottonwool.
Equal quantities of L and E solutions are mixed. The
pH is adjusted to 6.8 - 7.0 with 1 N NaOH. 100 ml of thesolution is dispensed into each 200ml-Erlenmayer flasks
and sterilized by autoclaving (121°C, 15 min).

In the NIES-Collectoin, ZnCl２ is replaced by
ZnSO４⋅7H２O.

PN metals

Soil extract (389)(390)

FeCl３･6H２O
MnCl２･4H２O
ZnSO４･7H２O１）
CoCl２･6H２O
Na２MoO４･2H２O
Na２EDTA･2H２O
Distilled water

63.

mg
mg
mg
mg
mg
mg
mg
mg
ml

100g soil combined with 100ml distilled water is heatedfor 2h and then cooled. The supernatant is passed througha GF / C filter and then distilled water added until there isa total of 100ml.

4)

1)

5
1
1
0.1
0.01
50
0.02
30
100

(387)

H３BO３
FeCl３･6H２O
MnSO４･4H２O
ZnSO４･7H２O
CoSO４･7H２O
Na２EDTA･2H２O
Distilled water

62. P IV metals

Vitamine mix S3 (387)

(541)
100
mg
113
mg
6.3
mg
0.093 mg4.66 mg
3.2
mg
100
ml

- 58 -

VII.

保存株データの利用法

系統保存株の学名はアルファベット順に並べてあり、学名が同じ場合は株番号順に並べてある。同定者が記載されていない限り、学名は原則として分離者によってつけられたものである。また、株番号は、数字の前に “NIES-” を付けて使用することとし(例:NIES-1)、株の学名が命名法などの変更で変わった場合や、やむをえない理由で株が消失した場合にも変更したり付け変えたりしないものとする。
個々の項目についての説明は下記の例を参照されたい。
Spirulina platensis (Gomont) Geitler1)
Syn. Arthrospira platensis Gomont2)
453)
Lake Kasumigaura / Ibaraki4) (1975-11)5)
IAM M-1846), Unialgal, Clonal7), M.M.Watanabe8) (1975-11)9)
Identified by: M.M.Watanabe10)
Culture conditions: MA, 25ﾟC, 24 µE / m2 sec, 1M, [Cryopreserved]11)
Characteristics: Water bloom, Freshwater,
Forming water bloom in Inbanuma12)
KAS-6-5013)
References: 79, 96, 242, 450, 518, 522, 530, 549, 55914)
1) 学名と原著者名：原著者名は学名の後に記した。
2) 異名。
3) 株番号：数字の前に “NIES-” を付けて使用すること。
4) 採集地。
5) 採集年月。
6) 他の保存機関に保存されている場合の株番号。保存機関名は略号で株番号の前に記されている。
IAM： 東京大学分子細胞生物学研究所

TAC： 国立科学博物館筑波実験植物園

CCAP： 英国 CCAPカルチャーコレクション

NIVA： ノルウェー 水界研究所藻類株保存施設

PCC： フランス パスツール研究所カルチャーコレクション

SAG： ドイツ ゲッチンゲン大学藻類株保存施設

CCMP： 米国 ビゲロウ海洋研究所カルチャーコレクション

UTEX： 米国 テキサス大学藻類株保存施設- 59 -

7) 株の状態。

Axenic の表示があるものは無菌株である。この表示のない場合は無菌ではないので特に注意されたい。
8) 分離者。
9) 分離年月。
10) 同定者。
11) 保存条件。培地名、保存温度、保存光強度、継代周期の順である。本施設では明暗周期は12時間明期／12時間暗期に設定されている。培地は特に記さない限り液体である。軟寒天培地：SS、寒天斜面培地：Sの場合は略号を（また（）内に記した。

）内の温度および光強度は前培養が必要な場合、その条件である。

なお光強度の表記については、前第６版より µE / m2 sec を採用している。
現在凍結保存中の株については [Cryopreserved] と記した。
12) 株の性質。

Unstable; 保存状態が不安定で永続的な維持が困難である株。
Untransportable; 長時間の（航空便での）郵送では、生存状態で受け取るのが困難である株。
13) 分離者等の使用している株名。
14) 参考文献の番号。
なお、第Ⅸ章 2. 分類群別索引（211〜224頁）における藻類門・綱の分類および配列は
千原光雄（編）「藻類の多様性と系統」（バイオダイバーシティ・シリーズ、裳華房 1999）に掲載された分類表におおむねしたがった。

- 60 -

VII. EXPLANATORY NOTES ABOUT STRAIN DATA
The strains are listed by scientific names in alphabetical order.

Strains with the samescientific name are arranged in order of their strain numbers.

The scientific name of each strain

was designated by the isolator, unless the identifier is described.

The number assigned to the given

strain remains the same, regardless of any change in nomenclature. The strain number should be
used with the initials "NIES-"(e.g. NIES-1).

A detailed example of a strain description is presented

below.

Spirulina platensis (Gomont) Geitler1)
Syn. Arthrospira platensis Gomont2)
453)
Lake Kasumigaura / Ibaraki4) (1975-11)5)
IAM M-1846), Unialgal, Clonal7), M.M.Watanabe8) (1975-11)9)
Identified by: M.M.Watanabe10)
Culture conditions: MA, 25ﾟC, 24 µE / m2 sec, 1M, [Cryopreserved]11)
Characteristics: Water bloom, Freshwater,
Forming water bloom in Inbanuma12)
KAS-6-5013)
References: 79, 96, 242, 450, 518, 522, 530, 549, 55914)

1)

Scientific name with authority.

2)

Synonym.

3)

Strain number (used with the initials "NIES-").

4) Collection site.
5)

Collection date.

6)

The strain designations in other culture collections or institutions.

The following abbreviations

are presented before the strain number.
IAM: Institute of Molecular and Cellular Biosciences, University of Tokyo.

TAC: Tsukuba Botanical Garden, National Science Museum.

CCAP : Culture Collection of Algae and Protozoa, U.K.
NIVA : Culture Collection of Algae, Norwegian Institute for Water Research.
PCC: Pasteur Culture Collection of Cyanobacteria, Institute Pasteur, France.

SAG: Culture Collection of Algae at the University of Göttingen, Germany.

CCMP : Provasoli-Guillard Center for Culture of Marine Phytoplankton, U.S.A.

- 61 -UTEX : Culture Collection of Algae at the University of Texas at Austin, U.S.A.
7)

Status of the strain (Unialgal or Axenic, Clonal or Non-clonal).

8)

Isolator.

9)

Isolation date.

10)

Identifier.

11)

Culture condition for maintenance: medium＊,temperature, light intensity＊＊ and durationof subculturing＊＊＊.
The light-dark cycle is defined as 12 hours light 12 hours dark.
＊

Unless otherwise noted the phase of the medium is liquid.
The abbreviations in parentheses are SS for semi-solid and S for solid.

＊＊

Light intensity is indicated as µE / m2 sec in this edition.

＊＊＊ Preculture temperature and light intensity are given in parentheses when preculture isrequired.
" [Cryopreserved] " indicates that the strain is preserved as a frozen condition at present.
12)

Characteristics of the strain.
"Unstable" indicates that the strain probably cannot be maintained indefinitely, for various
reasons including unsuccessful induction of auxospore formation and germination in diatom.
"Untransportable" indicates that the strain is not robust enough to be sent by air mail,involving much time.

13)

Strain designation given by the isolator.

14)

Reference number. References corresponding to the numbers are listed in pp.135~158.

Special Note.

Algal phyla and classes and assignment of strains to each taxon (as shown in Chap.

IX. 2. Systematic Index (pp.211~224) are arranged according principally to the system in Chihara(Ed.), “Diversity and Evolution of Algae” (Shokabo, Tokyo, 1999).

- 62 -

Appendix I. List of cryopreserved strains

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa

Cyanophyceae
Anabaenopsis circularis
Aphanocapsa montana
Aulosira laxa
Calothrix brevissima
Calothrix crustacea
Calothrix parasitica
Calothrix parasitica
Calothrix scopulorum
Chamaesiphon subglobosus
Limnothrix redekei
Lyngbya hieronymusii var. hieronymusii
Merismopedia tenuissima
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa

21
416
50
22
266
267
334
268
434
847
929
230
44
87
88
89
90
91
98
99
100
101
298
299
478
103
104
105
106
107
108
109
110
111
112
604
843
901
902
903
904
1025
1026
1027
1028
1029
1043
1050
1051

- 188 -

1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1069
1070
1071
1072
1073
1074
1077
1078
1079
1080
1081
1083
1084
1087
1088
1089
1090
1091
1094
1095
1096
1098
1099
1100
1101
1102
1103
1104
1105
1106
1107
1108
1109
1110
1111
1112

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Myxosarcina burmensis
Nostoc commune
Nostoc commune
Nostoc linckia var. arvense
Oscillatoria amphibia
Oscillatoria animalis
Oscillatoria limnetica
Oscillatoria rosea
Oscillatoria tenuis
Phormidium foveolarum
Phormidium foveolarum
Phormidium tenue
Phormidium tenue
Phormidium tenue
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix pseudoagardhii
Planktothrix pseudoagardhii
Planktothrix pseudoagardhii
Planktothrix pseudoagardhii
Planktothrix rubescens
Planktothrix rubescens
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.

1115
1117
1118
1119
1121
1122
1129
1130
1132
481
24
38
28
361
206
36
208
33
32
34
30
512
611
204
205
594
595
596
905
906
907
908
909
910
989
990
844
911
912
913
845
914
915
916
610
928
937
938
939
940
941
942
943
944
945
946
947

Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Tolypothrix tenuis
Tychonema bourrelii

948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
969
970
971
972
973
974
975
976
977
978
979
980
981
982
983
984
985
986
987
988
37
846

Rhodophyceae
Porphyridium sp.
Porphyridium sp.
Porphyridium sp.
Porphyridium sp.
Rhodella sp.
Rhodella sp.

- 189 -

1032
1033
1034
1035
1036
1037

Appendix II. List of toxic strains

Cyanophyceae
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Planktothrix agardhii

991
992
993
1040
1041
1042
1259
1260
1261
1262
88
89
90
102
103
107
298
478
843
901
902
903
904
933
1025
1026
1027
1028
1029
1043
1064
1070
1071
1072
1077
1085
1086
1095
1097
1099
1140
1141
905

Planktothrix agardhii
Planktothrix agardhii
Planktothrix rubescens
Planktothrix rubescens
Planktothrix rubescens

1263
1264
928
1266
1267

Dinophyceae
Alexandrium hiranoi
Coolia monotis
Prorocentrum lima

- 190 -

612
615
617

Appendix III. List of gene data

1067 Microcystis aeruginosa
1068 Microcystis aeruginosa
1068 Microcystis aeruginosa
1072 Microcystis aeruginosa
1072 Microcystis aeruginosa
1076 Microcystis aeruginosa
1085 Microcystis aeruginosa
1085 Microcystis aeruginosa
1086 Microcystis aeruginosa
1090 Microcystis aeruginosa
1090 Microcystis aeruginosa
1090 Microcystis aeruginosa
1091 Microcystis aeruginosa
1091 Microcystis aeruginosa
1092 Microcystis aeruginosa
1105 Microcystis aeruginosa
1105 Microcystis aeruginosa
1115 Microcystis aeruginosa
1117 Microcystis aeruginosa
1122 Microcystis aeruginosa
1133 Microcystis aeruginosa
1142 Microcystis aeruginosa
1143 Microcystis aeruginosa
1143 Microcystis aeruginosa
1164 Microcystis aeruginosa
33 Oscillatoria tenuis
30 Phormidium tenue
512 Phormidium tenue
611 Phormidium tenue
917 Planktothricoides raciborskii
918 Planktothricoides raciborskii
919 Planktothricoides raciborskii
204 Planktothrix agardhii
205 Planktothrix agardhii
594 Planktothrix agardhii
595 Planktothrix agardhii
596 Planktothrix agardhii
905 Planktothrix agardhii
906 Planktothrix agardhii
907 Planktothrix agardhii
844 Planktothrix mougeotii
911 Planktothrix mougeotii
912 Planktothrix mougeotii
913 Planktothrix mougeotii
845 Planktothrix pseudoagardhii
914 Planktothrix pseudoagardhii
915 Planktothrix pseudoagardhii
916 Planktothrix pseudoagardhii
610 Planktothrix rubescens
945 Synechococcus sp.
951 Synechococcus sp.

16S rRNA gene
Cyanophyceae
41 Anabaena circinalis
73 Anabaena flos-aquae
f. flos-aquae
76 Anabaena spiroides
21 Anabaenopsis circularis1031 Chroogloeocystis siderophila
847 Limnothrix redekei
929 Lyngbya hieronymusii
var. hieronimusii
44 Microcystis aeruginosa
87 Microcystis aeruginosa88 Microcystis aeruginosa
89 Microcystis aeruginosa
90 Microcystis aeruginosa
91 Microcystis aeruginosa
98 Microcystis aeruginosa
99 Microcystis aeruginosa
100 Microcystis aeruginosa
101 Microcystis aeruginosa
102 Microcystis aeruginosa
104 Microcystis aeruginosa
104 Microcystis aeruginosa
104 Microcystis aeruginosa
105 Microcystis aeruginosa
106 Microcystis aeruginosa
107 Microcystis aeruginosa
108 Microcystis aeruginosa
109 Microcystis aeruginosa
110 Microcystis aeruginosa
111 Microcystis aeruginosa
111 Microcystis aeruginosa
112 Microcystis aeruginosa
112 Microcystis aeruginosa
298 Microcystis aeruginosa
299 Microcystis aeruginosa
604 Microcystis aeruginosa
843 Microcystis aeruginosa
1054 Microcystis aeruginosa
1054 Microcystis aeruginosa
1055 Microcystis aeruginosa
1055 Microcystis aeruginosa
1055 Microcystis aeruginosa
1058 Microcystis aeruginosa
1061 Microcystis aeruginosa
1062 Microcystis aeruginosa
1062 Microcystis aeruginosa
1067 Microcystis aeruginosa

AB042859
AB042858
AB047104
AB043537
AY380791
AB045929
AB045906
AB015361
D89031
AB023255
U03403
AB023256
AB023257
D89032
AB023258
AB023259
AB023260
D89033
AB015387
AB023266
AJ133174
AB023267
AB023268
U40333
AB023269
AB023270
AB023271
D89034
AB015388
U40334
AB023272
AB023261
AB023262
AB023273
AB035549
AB012336
AB015374
AB012334
AB015389
AB023274
AB015400
AB023281
AB015400
AB023276
AB012337- 191 -

AB023285
AB015376
AB023286
AB012332
AB015362
AB023287
AB012333
AB015363
AB015364
AB012339
AB015367
AB023282
AB015402
AB023278
AB015403
AB015368
AB023283
AB015369
AB015370
AB023284
AB023263
AB023264
AB012340
AB015365
AB023265
AB042844
AB042857
AB042838
AB042842
AB045953
AB045967
AB045964
AB045954
AB045955
AB045956
AB045957
AB045958
AB045896
AB045904
AB045905
AB045971
AB045972
AB045969
AB045970
AB045968
AB045907
AB045965
AB045966
AB045959
AF216951
AF216952

953 Synechococcus sp.
957 Synechococcus sp.
959 Synechococcus sp.
969 Synechococcus sp.
970 Synechococcus sp.
971 Synechococcus sp.
972 Synechococcus sp.
973 Synechococcus sp.
978 Synechococcus sp.
979 Synechococcus sp.
981 Synechococcus sp.
984 Synechococcus sp.
846 Tychonema bourrellyi

185 Closterium pusillum
339 Closterium selenastrum
187 Closterium spinosporum
var. crassum
188 Closterium spinosporum
var. malaysiense
191 Closterium spinosporum
var. ryukyuense
194 Closterium spinosporum
var. spinosporum
198 Closterium tumidum
199 Closterium venus
200 Closterium wallichii

AF216953
AF216954
AF216955
AF448060
AF448073
AF448061
AF448079
AF448062
AF448063
AF448074
AF448064
AF448066
AB045897

AF352235
AF352242
AF352241
AF352227
AF352240
AF352224
AF352234
AF352236
AF352243

Placididea
1015 Wobblia lunata

AB032606

16S-23S ITS region
atpB gene

Cyanophyceae
44 Microcystis aeruginosa
104 Microcystis aeruginosa
111 Microcystis aeruginosa

AB015361
AB015387
AB015388

Chlorophyceae
418 Astrephomene gubernaculifera
854 Astrephomene gubernaculifera
564 Astrephomene perforata
566 Basichlamys sacculifera
425 Carteria cerasiformis
421 Carteria crucifera
428 Carteria obtusa
432 Carteria radiosa
567 Characiochloris sasae
884 Chlamydomonas debaryana
var. cristata
968 Chlamydomonas kuwadae
1048 Chlamydomonas noctigama
446 Chlamydomonas tetragama
123 Chlorogonium fusiforme
439 Chlorogonium neglectum
447 Chloromonas insignis
722 Eudorina cylindrica
721 Eudorina elegans var. carteri
456 Eudorina elegans var. elegans
717 Eudorina elegans var. elegans
718 Eudorina elegans var. elegans
719 Eudorina elegans var. elegans
720 Eudorina elegans var. elegans
568 Eudorina elegans var. synoica
460 Eudorina illinoisensis
723 Eudorina illinoisensis
856 Eudorina minodii
726 Eudorina unicocca
var. peripheralis
724 Eudorina unicocca
var. unicocca
725 Eudorina unicocca
var. unicocca
737 Gonium multicoccum
851 Gonium octonarium

18S rRNA gene
Cryptophyceae
715 Chilomonas paramecium
274 Cryptomonas ovata

AB073108
AB073109

Phaeophyceae
548 Acinetospora crinita

AF038005

Schizocladiophyceae
1044 Schizocladia ischiensis

AB085614

Ulvophyceae
360 Oltmannsiellopsis viridis

D86495

Trebouxiophyceae
996 Stichococcus ampulliformis

AB087559

Chlorophyceae
858 Phacotus lenticularis

X91628Charophyceae
125 Closterium acerosum
AF352230
271 Closterium calosporum
var. calosporum
AF352225
165 Closterium calosporum
var. galiciense
AF352239
170 Closterium calosporum
var. himalayense
AF352229
180 Closterium gracile
AF352237
181 Closterium incurvum
AF352231
174 Closterium moniliferum
var. moniliferum
AF352233
175 Closterium navicula
AF352232
52 Closterium peracerosumstrigosum-littorale complex AF352226
449 Closterium pleurodermatum
AF352238- 192 -

AB014022-3
AB044181
AB014024
AB014015
AB084321
AB084320
AB084323
AB084322
AB084331
AB014034
AB084318
AB101502
AB084319
AB084329
AB084326
AB084313
AB014033
AB014012
AB014009
AB047071
AB047072
AB047073
AB014010
AB014011
AB014013
AB047069
AB047068
AB047070
AB014008
AB014007
AB014020
AB014018

569 Gonium pectorale var. pectorale AB014016-7
653 Gonium quadratum
AB014019
289 Gonium viridistellatum
AB076118-9
654 Gonium viridistellatum
AB014021
857 Gonium viridistellatum
AB076117144 Haematococcus lacustris
AB084325
257 Hafniomonas montana
AB101504
656 Hafniomonas montana
AB101505
474 Lobomonas monstuosa
AB044533
572 Pandorina colemaniae
AB014027
886 Pandorina morum
AB044180887 Pandorina morum
AB044179
889 Pandorina morum
AB044178
890 Pandorina morum
AB044177574 Pandorina morum var. morum AB014025-6
727 Paulschulzia pseudovolvox
AB014040
213 Pediastrum duplex
AB084306
858 Phacotus lenticularis
AB014039
728 Platydorina caudata
AB014032
735 Pleodorina californica
AB014004
736 Pleodorina indica
AB014006
522 Pseudocarteria mucosa
AB084324
861 Pteromonas angulosa
AB014038
96 Scenedesmus quadricauda
AB084305
571 Tetrabaena socialis var. socialis AB014014
875 Vitreochlamys aulata
AB076121882 Vitreochlamys ordinata
AB014036
883 Vitreochlamys pinguis
AB076120541 Volvox aureus var. aureus
AB013998
891 Volvox aureus
AB076104892 Volvox aureus
AB076105730 Volvox barberi
AB014001
732 Volvox carteri f. kawasakiensis AB013999
731 Volvox dissipatrix
AB014000734 Volvox rousseletii
AB014003
893 Volvulina boldii
AB044176582 Volvulina compacta
AB014029
895 Volvulina pringsheimii
AB014028
545 Volvulina steinii
AB044713
896 Volvulina steinii
AB044174898 Volvulina steinii
AB044175
666 Yamagishiella unicocca
AB014030
872 Yamagishiella unicocca
AB044172388 Phaeocystis globosa
12 Prorocentrum micans
369 Scrippsiella trochoidea

AB000133-4
AB000135

Euglenophyceae
381 Eutreptiella gymnastica

AB000136

Trebouxiophyceae
415 Actinastrum hantzschii
D63660227 Chlorella vulgaris var. vulgaris D63763
227 Chlorella vulgaris var. vulgaris AB011523

Chlorophyceae
209 Pediastrum boryanum
96 Scenedesmus quadricauda
96 Scenedesmus quadricauda

D63659D63658AB011524

cpcBA-IGS gene
Cyanophyceae
937 Synechococcus sp.
938 Synechococcus sp.
939 Synechococcus sp.
940 Synechococcus sp.
941 Synechococcus sp.
942 Synechococcus sp.
943 Synechococcus sp.
944 Synechococcus sp.
945 Synechococcus sp.
946 Synechococcus sp.
947 Synechococcus sp.
948 Synechococcus sp.
949 Synechococcus sp.
950 Synechococcus sp.
951 Synechococcus sp.
952 Synechococcus sp.
953 Synechococcus sp.
954 Synechococcus sp.
955 Synechococcus sp.
956 Synechococcus sp.
957 Synechococcus sp.
958 Synechococcus sp.
959 Synechococcus sp.
960 Synechococcus sp.
961 Synechococcus sp.
962 Synechococcus sp.
963 Synechococcus sp.
964 Synechococcus sp.
965 Synechococcus sp.

COXI gene
Cryptophyceae
274 Cryptomonas ovata

AB000120

Dinophyceae

AB009419

AF223433
AF223434
AF223438
AF223452
AF223439
AF223440
AF223441
AF223453
AF223428
AF223443
AF223444
AF223454
AF223455
AF223448
AF223429
AF223449
AF223430
AF223456
AF223445
AF223450
AF223431
AF223435
AF223432
AF223436
AF223446
AF223442
AF223447
AF223437
AF223451

Raphidophyceae
1 Chattonella antiqua

AF037990

Phaeophyceae
548 Acinetospora crinita

gyrB gene

AF037996

Cyanophyceae

Prymnesiophyceae
8 Cricosphaera roscoffensis
353 Gephyrocapsa oceanica

104 Microcystis aeruginosa

AB000117
AB000118- 193 -

AB074771

736 Pleodorina indica
AB044195-7
571 Tetrabaena socialis var. socialis AB044415
875 Vitreochlamys aulata
AB076143
882 Vitreochlamys ordinata
AB044420
883 Vitreochlamys pinguis
AB076142
891 Volvox aureus
AB076123892 Volvox aureus
AB076124541 Volvox aureus var. aureus
AB044182
730 Volvox barberi
AB044186
732 Volvox carteri f. kawasakiensis AB044184-5
731 Volvox dissipatrix
AB044183734 Volvox rousseletii
AB044188
893 Volvulina boldii
AB044225
582 Volvulina compacta
AB044217-9
895 Volvulina pringsheimii
AB044220
545 Volvulina steinii
AB044221-2
896 Volvulina steinii
AB044223
898 Volvulina steinii
AB044224
666 Yamagishiella unicocca
AB044213
872 Yamagishiella unicocca
AB044216

Mitochondrial complete DNA
Prasinophyceae
296 Mesostigma viride
484 Nephroselmis olivacea

AF353999
AF110138

petG, psbK, D, psaI etc.
Bacillariophyceae
323 Skeletonema costatum

AJ132266

Plastid complete DNA
Prasinophyceae
296 Mesostigma viride
484 Nephroselmis olivacea

AF166114
AF137379

psaA gene

psaB gene

Chlorophyceae
418 Astrephomene gubernaculifera AB044233-4
854 Astrephomene gubernaculifera AB044235
564 Astrephomene perforata
AB044236-8
566 Basichlamys sacculifera
AB044416
884 Chlamydomonas debaryana
var. cristata
AB044417-8
722 Eudorina cylindrica
AB044210
721 Eudorina elegans var. carteri
AB044202-3
456 Eudorina elegans var. elegans AB044199
720 Eudorina elegans var. elegans AB044200-1
460 Eudorina illinoisensis
AB044198
724 Eudorina unicocca
var. unicocca
AB044204-6
725 Eudorina unicocca
var. unicocca
AB044207-9
737 Gonium multicoccum
AB044239-40
851 Gonium octonarium
AB044241
569 Gonium pectorale var. pectorale AB044242
653 Gonium quadratum
AB044243
289 Gonium viridistellatum
AB076140-1
654 Gonium viridistellatum
AB044244
857 Gonium viridistellatum
AB076139
474 Lobomonas monstuosa
AB044421
572 Pandorina colemaniae
AB044232
886 Pandorina morum
AB044231
887 Pandorina morum
AB044229-30
889 Pandorina morum
AB044228
890 Pandorina morum
AB044227574 Pandorina morum var. morum AB044226
727 Paulschulzia pseudovolvox
AB044422-3
728 Platydorina caudata
AB044211-2
735 Pleodorina californica
AB044190-2- 194 -

Chlorophyceae
418 Astrephomene gubernaculifera AB044458
854 Astrephomene gubernaculifera AB044459
564 Astrephomene perforata
AB044460
566 Basichlamys sacculifera
AB044467-8
425 Carteria cerasiformis
AB084359
421 Carteria crucifera
AB084358
428 Carteria obtusa
AB084361-3
432 Carteria radiosaAB084360
567 Characiochloris sasae
AB084376
884 Chlamydomonas debaryana
var. cristata
AB044469
968 Chlamydomonas kuwadae
AB084356
1048 Chlamydomonas noctigama
AB101513
446 Chlamydomonas tetragama
AB084357
123 Chlorogonium fusiforme
AB084370
439 Chlorogonium neglectum
AB084366
447 Chloromonas insignis
AB084348
722 Eudorina cylindrica
AB044441
721 Eudorina elegans var. carteri
AB044438
456 Eudorina elegans var. elegans AB044435
720 Eudorina elegans var. elegans AB044436-7
460 Eudorina illinoisensis
AB044434
724 Eudorina unicocca
var. unicocca
AB044439
725 Eudorina unicocca
var. unicocca
AB044440
737 Gonium multicoccum
AB044461
851 Gonium octonarium
AB044462
569 Gonium pectorale var. pectorale AB044463
653 Gonium quadratum
AB044464
289 Gonium viridistellatum
AB076156

654 Gonium viridistellatum
AB044465
857 Gonium viridistellatum
AB076155
144 Haematococcus lacustris
AB084365
257 Hafniomonas montana
AB101515
656 Hafniomonas montana
AB101516
474 Lobomonas monstuosa
AB044472
572 Pandorina colemaniae
AB044457
886 Pandorina morum
AB044456
887 Pandorina morum
AB044455
889 Pandorina morum
AB044454
890 Pandorina morum
AB044453
574 Pandorina morum var. morum AB044452
727 Paulschulzia pseudovolvox
AB044473
213 Pediastrum duplex
AB084340
858 Phacotus lenticularis
AB084373-4
728 Platydorina caudata
AB044442
735 Pleodorina californica
AB044430
736 Pleodorina indica
AB044432-3
522 Pseudocarteria mucosa
AB084364
861 Pteromonas angulosa
AB084371-2
96 Scenedesmus quadricauda
AB084339
571 Tetrabaena socialis var. socialis AB044466
875 Vitreochlamys aulata
AB076158882 Vitreochlamys ordinata
AB044471
883 Vitreochlamys pinguis
AB076157
891 Volvox aureus
AB076145
892 Volvox aureus
AB076146
541 Volvox aureus var. aureus
AB044424
730 Volvox barberi
AB044427
732 Volvox carteri f. kawasakiensis AB044425
731 Volvox dissipatrix
AB044426
734 Volvox rousseletii
AB044429
893 Volvulina boldii
AB044451
582 Volvulina compacta
AB044446
895 Volvulina pringsheimii
AB044447
545 Volvulina steinii
AB044448
896 Volvulina steinii
AB044449
898 Volvulina steinii
AB044450
666 Yamagishiella unicocca
AB044443
872 Yamagishiella unicocca
AB044445

psbA gene
Dinophyceae
331 Amphidinium carterae
12 Prorocentrum micans

psbC gene
Chlorophyceae
418 Astrephomene gubernaculifera AB044513-4
854 Astrephomene gubernaculifera AB044515-7
564 Astrephomene perforata
AB044518-9
566 Basichlamys sacculifera
AB044526
884 Chlamydomonas debaryana
var. cristata
AB044527
722 Eudorina cylindrica
AB044493
721 Eudorina elegans var. carteri
AB044487-8
456 Eudorina elegans var. elegans AB044485
720 Eudorina elegans var. elegans AB044486
460 Eudorina illinoisensis
AB044484
724 Eudorina unicocca
var. unicocca
AB044489-90
725 Eudorina unicocca
var. unicocca
AB044491-2
737 Gonium multicoccum
AB044481
851 Gonium octonarium
AB044520
569 Gonium pectorale var. pectorale AB044521
653 Gonium quadratum
AB044522-3
289 Gonium viridistellatum
AB076173
654 Gonium viridistellatum
AB044524
857 Gonium viridistellatum
AB076172
474 Lobomonas monstuosa
AB044530572 Pandorina colemaniae
AB044512
886 Pandorina morum
AB044510-1
887 Pandorina morum
AB044509
889 Pandorina morum
AB044508
890 Pandorina morum
AB044506-7
574 Pandorina morum var. morum AB044505
727 Paulschulzia pseudovolvox
AB044531-2
728 Platydorina caudata
AB044494
735 Pleodorina californica
AB044480
736 Pleodorina indica
AB044483
571 Tetrabaena socialis var. socialis AB044525
875 Vitreochlamys aulata
AB076175-7
882 Vitreochlamys ordinata
AB044529
883 Vitreochlamys pinguis
AB076174891 Volvox aureus
AB076160
892 Volvox aureus
AB076161
541 Volvox aureus var. aureus
AB044474
730 Volvox barberi
AB044477
732 Volvox carteri f. kawasakiensis AB044475
731 Volvox dissipatrix
AB044476
734 Volvox rousseletii
AB044479
893 Volvulina boldii
AB044504
582 Volvulina compacta
AB044498

psaC, psbA etc.
Bacillariophyceae
323 Skeletonema costatum

AJ132264

psaD gene
Glaucophyceae
547 Cyanophora paradoxa

AB025586
AB025585

AJ132477- 195 -

895 Volvulina pringsheimii
545 Volvulina steinii
896 Volvulina steinii
898 Volvulina steinii
666 Yamagishiella unicocca
872 Yamagishiella unicocca

AB044499
AB044500
AB044501
AB044502-3
AB044495
AB044497

psbC, D, petB, D etc.
Bacillariophyceae
323 Skeletonema costatum

AJ132263

rbcL gene
Schizocladiophyceae
1044 Schizocladia ischiensis

AB085615

Chlorophyceae
418 Astrephomene gubernaculifera
854 Astrephomene gubernaculifera
564 Astrephomene perforata
566 Basichlamys sacculifera
424 Carteria cerasiformis
425 Carteria cerasiformis
421 Carteria crucifera
630 Carteria crucifera
631 Carteria eugametos
632 Carteria eugametos
633 Carteria eugametos
634 Carteria eugametos
635 Carteria eugametos
636 Carteria eugametos
422 Carteria inversa
423 Carteria inversa
428 Carteria obtusa
432 Carteria radiosa
567 Characiochloris sasae
884 Chlamydomonas debaryana
var. cristata
968 Chlamydomonas kuwadae
1048 Chlamydomonas noctigama
446 Chlamydomonas tetragama
692 Chlorogonium capillatum
742 Chlorogonium capillatum
743 Chlorogonium capillatum
744 Chlorogonium capillatum
745 Chlorogonium capillatum
746 Chlorogonium capillatum
747 Chlorogonium capillatum
748 Chlorogonium capillatum
749 Chlorogonium capillatum
750 Chlorogonium capillatum
751 Chlorogonium elongatum
752 Chlorogonium elongatum

D63428AB044169-70
D63429D63430D89767
D89768
D63431
D89758
D89762
D89763
D89764
D89761
D89759D89760D89765
D89766
D89769D89770AB084338
D86838AB084334
AB101506-7
AJ001880
AB010230
AB010234
AB010235
AB010236
AB010231
AB010232
AB010233
AB010237
AB010238
AB010239
AJ001881
AB010240- 196 -

753 Chlorogonium elongatum
AB010241754 Chlorogonium euchlorum
AB010226
755 Chlorogonium euchlorum
AB010227756 Chlorogonium euchlorum
AB010228
757 Chlorogonium euchlorum
AB010224
758 Chlorogonium euchlorum
AJ001882
759 Chlorogonium euchlorum
AB010225
760 Chlorogonium euchlorum
AB010229
123 Chlorogonium fusiforme
AB010242
761 Chlorogonium kasakii
AB010244
439 Chlorogonium neglectum
AB010243
447 Chloromonas insignis
AB022226
722 Eudorina cylindrica
D86833721 Eudorina elegans var. carteri
D88806456 Eudorina elegans var. elegans D63432
717 Eudorina elegans var. elegans D88803
718 Eudorina elegans var. elegans D88810
719 Eudorina elegans var. elegans D88804
720 Eudorina elegans var. elegans D88805
458 Eudorina elegans var. synoica D88807
568 Eudorina elegans var. synoica D88808
460 Eudorina illinoisensis
D63433723 Eudorina illinoisensis
D88809856 Eudorina minodii
AB047074-6
726 Eudorina unicocca
var. peripheralis
D86830724 Eudorina unicocca
var. unicocca
D86829725 Eudorina unicocca
var. unicocca
D63434737 Gonium multicoccum
D63435569 Gonium pectorale var. pectorale D63437
653 Gonium quadratum
D63438289 Gonium viridistellatum
AB076091
654 Gonium viridistellatum
D86831857 Gonium viridistellatum
AB076092-3
144 Haematococcus lacustris
AB084336-7
257 Hafniomonas montana
AB101509-10
656 Hafniomonas montana
AB101511-2
474 Lobomonas monstuosa
AB044171572 Pandorina colemaniae
D63441
886 Pandorina morum
AB044167887 Pandorina morum
AB044166
889 Pandorina morum
AB044165
890 Pandorina morum
AB044164574 Pandorina morum var. morum D63442
727 Paulschulzia pseudovolvox
D86837
213 Pediastrum duplex
AB084333
858 Phacotus lenticularis
AJ001883
859 Phacotus lenticularis
AJ001884
728 Platydorina caudata
D86828729 Platydorina caudata
D86827735 Pleodorina californica
D63439736 Pleodorina indica
D86834577 Pleodorina japonica
D63440522 Pseudocarteria mucosa
AB084335
861 Pteromonas angulosa
AJ001887

862 Pteromonas angulosa
AJ001888
96 Scenedesmus quadricauda
AB084332
571 Tetrabaena socialis var. socialis D63443
875 Vitreochlamys aulata
AB050486-7
876 Vitreochlamys aulata
AB050488-9
877 Vitreochlamys aulata
AB050492
878 Vitreochlamys aulata
AB050493
879 Vitreochlamys fluviatilis
AB050484
880 Vitreochlamys gloeocystiformis AB050485
881 Vitreochlamys nekrassovii
AB050494
882 Vitreochlamys ordinata
AB014041
883 Vitreochlamys pinguis
AB050490-1
891 Volvox aureus
AB076096
892 Volvox aureus
AB076086
541 Volvox aureus var. aureus
D63445730 Volvox barberi
D86835732 Volvox carteri f. kawasakiensis D63446
731 Volvox dissipatrix
D63447734 Volvox rousseletii
D63448893 Volvulina boldii
AB044162-3
582 Volvulina compacta
D86832895 Volvulina pringsheimii
D63444545 Volvulina steinii
AB044159
896 Volvulina steinii
AB044160
898 Volvulina steinii
AB044161
578 Yamagishiella uniclcca
AB000811
666 Yamagishiella uniclcca
D86823762 Yamagishiella uniclcca
AB000810
872 Yamagishiella unicocca
AB044168979 Synechococcus sp.
980 Synechococcus sp.
981 Synechococcus sp.
982 Synechococcus sp.
983 Synechococcus sp.
984 Synechococcus sp.
985 Synechococcus sp.

AF448109
AF448097
AF448085
AF448113
AF448104
AF448087
AF448102

rpoD1 gene
Cyanophyceae
104 Microcystis aeruginosa

AB074821

trnD, I, T etc.
Bacillariophyceae
323 Skeletonema costatum

AJ132265

tufA gene
Phaeophyceae
548 Acinetospora crinita

AF038004

ycf24, partialrbcL-462 intron

Bacillariophyceae
323 Skeletonema costatum

Chlorophyceae
418 Astrephomene gubernaculifera
289 Gonium viridistellatum
654 Gonium viridistellatum
857 Gonium viridistellatum
875 Vitreochlamys aulata
892 Volvox aureus
582 Volvulina compacta

AB076095
AB076091
AB076090
AB076092
AB076097
AB076086
AB076089

rpoC1 gene
Cyanophyceae
104 Microcystis aeruginosa
969 Synechococcus sp.
971 Synechococcus sp.
972 Synechococcus sp.
974 Synechococcus sp.
975 Synechococcus sp.
976 Synechococcus sp.
977 Synechococcus sp.
978 Synechococcus sp.

AB074794
AF448082
AF448083
AF448117
AF448101
AF448099
AF448100
AF448105
AF448084- 197 -AJ132267

IX. INDEXES

1. Numerical index

1
2
3
4
5
6
7
8
9
10
12
14
15
16
17
18
19
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
44
45
46
47
48

Chattonella antiqua
Chattonella antiqua
Chattonella marina
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterocapsa triquetra
Cricosphaera roscoffensis
Heterosigma akashiwo
Heterosigma akashiwo
Prorocentrum micans
Chattonella marina
Olisthodiscus luteus
Skeletonema costatum
Skeletonema costatum
Tetraselmis cordiformis
Anabaena cylindrica
Anabaenopsis circularis
Calothrix brevissima
Anabaena variabilis
Nostoc commune
Nostoc linckia
Nostoc minutum
Spirulina subsalsa
Nostoc linckia var. arvense
Nostoc minutum
Phormidium tenue
Oscillatoria laetevirens
Phormidium foveolarum
Oscillatoria tenuis
Phormidium foveolarum
Pseudokirchneriella subcaiptata
Oscillatoria limnetica
Tolypothrix tenuis
Nostoc commune
Spirulina platensis
Anabaena affinis
Anabaena circinalis
Microcystis aeruginosaSpirulina platensis
Spirulina platensis
Euglena gracilis
Euglena gracilis

49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70

- 198 -

Euglena gracilis var. bacillaris
Aulosira laxa
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex

71
73
74
75
76
77
78
79
80
81
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122

Achnanthes minutissima
Anabaena flos-aquae f. flos-aquae
Anabaena flos-aquae f. flos-aquae
Anabaena flos-aquae f. flos-aquae
Anabaena spiroides
Anabaena spiroides f. spiroides
Anabaena spiroides f. crassa
Anabaena spiroides f. spiroides
Anabaena solitaria f. solitaria
Aphanizomenon flos-aquae f. gracile
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Scenedesmus acuminatus var. tetradesmoides
Scenedesmus dimorphus
Scenedesmus acutus
Scenedesmus acutus
Scenedesmus quadricauda
Scenedesmus serratus
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Chattonella antiqua
Chattonella antiqua
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella marina
Scenedesmus dimorphus
Scenedesmus acutus
Chattonella marina
Chlamydomonas pulsatilla

123
124
125
127
128
129
130
131
132
133
134
135
136
137
138
139
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177

- 199 -

Chlorogonium fusiforme
Closterium acerosum
Closterium acerosum
Closterium acerosum
Closterium calosporum var. galiciense
Coelastrum astroideum
Coelastrum astroideum
Coelastrum proboscideum
Coelastrum reticulatum
Cosmarium contractum
Dimorphococcus lunatus
Dimorphococcus lunatus
Fibrocapsa japonica
Echinosphaeridium nordstedtii
Gonatozygon brebissonii
Gonatozygon brebissonii
Haematococcus lacustris
Heterosigma akashiwo
Heterosigma akashiwo
Hyalotheca dissiliens
Hyalotheca dissiliens
Hyalotheca dissiliens
Hyalotheca dissiliens
Micractinium pusillum
Micrasterias crux-melitensis
Chlorosarcinopsis delicata
Characium maximum
Tetracystis chlorococcoides
Urnella terrestris
Chlamydomonas monticola
Chlamydomonas augustae var. ellipsoidea
Pseudopleurococcus printzii var. longissimus
Chlorosarcinopsis caeca
Chattonella antiqua
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. himalayense
Closterium calosporum var. himalayense
Closterium calosporum var. himalayense
Closterium moniliferum var. moniliferum
Closterium moniliferum var. moniliferum
Closterium moniliferum var. moniliferum
Closterium navicula
Closterium navicula
Closterium navicula

178
179
180
181
182
183
185
186
187
188
189
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
223
224
225
226
227
228
229
230

Closterium navicula
Closterium gracile
Closterium gracile
Closterium incurvum
Closterium moniliferum var. submoniliferum
Closterium moniliferum var. submoniliferum
Closterium pusillum var. maius
Closterium spinosporum var. crassum
Closterium spinosporum var. crassum
Closterium spinosporum var. malaysiense
Closterium spinosporum var. malaysiense
Closterium spinosporum var. ryukyuense
Closterium spinosporum var. ryukyuense
Closterium spinosporum var. ryukyuense
Closterium spinosporum var. spinosporum
Closterium spinosporum var. spinosporum
Closterium spinosporum var. spinosporum
Closterium spinosporum var. spinosporum
Closterium tumidum
Closterium venus
Closterium wallichii
Closterium wallichii
Closterium wallichii
Oedogonium obesum
Planktothrix agardhii
Planktothrix agardhii
Oscillatoria animalis
Planktothricoides raciborskii
Oscillatoria rosea
Pediastrum boryanum
Pediastrum duplex var. duplex
Pediastrum duplex var. gracillimum
Pediastrum duplex
Pediastrum duplex var. duplex
Pediastrum duplex var. gracillimum
Pediastrum simplex
Pediastrum tetras
Penium margaritaceum
Prorocentrum micans
Prorocentrum triestinumAlexandrium catenella
Pterosperma cristatum
Skeletonema costatum
Staurastrum dejectum
Tabellaria flocculosa
Graesiella emersonii
Chlorella vulgaris var. vulgaris
Closterium ehrenbergii
Closterium ehrenbergii
Merismopedia tenuissima

231
232
233
234
235
237
238
241
242
243
244
245
246
247
248
251
252
253
254
255
256
257
258
259
261
262
263
265
266
267
268
271
274
275
276
277
278
279
280
281
282
284
285
286
287
288
289
290

- 200 -

Coelastrum morus
Polyedriopsis spinulosa
Synura petersenii
Synura spinosa
Heterocapsa triquetra
Prorocentrum minimum
Prorocentrum minimum
Volvox aureus
Pandorina morum
Pandorina morum
Coelastrum astroideum
Coelastrum reticulatum var. reticulatum
Schroederia setigera
Gonatozygon monotaenium
Cosmocladium constrictum
Pyramimonas aff. amylifera
Nephroselmis astigmatica
Euglena clara
Pyramimonas parkeae
Monomastix minuta
Monomastix minuta
Hafniomonas montana
Closterium aciculare var. subpronum
Closterium aciculare var. subpronum
Closterium peracerosum-strigosumlittorale complex
Closterium peracerosum-strigosumlittorale complex
Anabaena spiroides f. spiroides
Asterionella glacialis
Calothrix crustacea
Calothrix parasitica
Calothrix scopulorum
Closterium calosporum var. calosporum
Cryptomonas ovata
Cryptomonas ovata
Cryptomonas platyuris
Cryptomonas rostratiformis
Cryptomonas rostratiformis
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Dinobryon divergens
Docidium undulatum var. undulatum
Euglena mutabilis
Gonatozygon monotaenium
Gonium viridistellatum
Gonium viridistellatum
Gonium viridistellatum

293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
315
316
318
319
320
321
323
324
325
329
330
331
333
334
336
337
338
339
340
341
342
343
344
345
346
347
348

Heterosigma akashiwo
Hyalotheca dissiliens
var. dissiliens f. tridentula
Hydrodictyon reticulatum
Mesostigma viride
Micrasterias foliacea var. foliacea
Microcystis aeruginosa
Microcystis aeruginosa
Pediastrum angulosum var. angulosum
Pediastrum boryanum
Pediastrum simplex
Penium margaritaceum
Peridinium willei
Phormidium ramosum
Pleurotaenium cylindricum var. stuhlmannii
Pleurotaenium ehrenbergii var. curtum
Pleurotaenium ehrenbergii var. curtum
Pleurotaenium ehrenbergii var. ehrenbergii
Pleurotaenium ehrenbergii var. ehrenbergii
Pleurotaenium ehrenbergii var. curtum
Pleurotaenium nodosum var. nodosum
Pleurotaenium ovatum
Prorocentrum gracile
Prorocentrum micans
Protoceratium reticulatum
Protoceratium reticulatum
Pyramimonas aff. amylifera
Pyrophacus steinii
Skeletonema costatum
Skeletonema costatum
Spinoclosterium cuspidatum
Ulothrix variabilis
Achnanthes subconstricta
Amphidinium carterae
Melosira granulata
var. angustissima f. spiralis
Calothrix parasitica
Closterium calosporum var. himalayense
Closterium incurvum
Closterium rostratum var. subrostratum
Closterium selenastrum
Closterium selenastrum
Closterium spinosporum var. crassum
Coelastrum astroideum
Coolia monotis
Cryptomonas platyuris
Cryptomonas rostratiformis
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa

349
350
351
353
356
359
360
361
362
363
365
366
369
372
375
376
377
378
379
380
381
382
384
385
387
388
390
391
392
394
395
396
397
398
403
405
407
408
409
410
411
412
413
414
415
416
417
418
419
420

- 201 -

Cylindrocystis brebissonii var. brebissonii
Ditylum brightwellii
Eudorina elegans
Gephyrocapsa oceanica
Katodinium rotundatum
Oltmannsiellopsis unicellularis
Oltmannsiellopsis viridis
Oscillatoria amphibia
Pandorina morum
Pedinomonas minor
Peridinium volzii
Peridinium willei
Scrippsiella trochoidea
Achnanthes minutissima var. saprophila
Brachiomonas submarina
Ceratium hirundinella
Chaetoceros sociale
Dictyochloropsis irregularis
Eremosphaera gigas
Eremosphaera viridis
Eutreptiella gymnastica
Lagerheimia ciliata
Monoraphidium contortum
Monoraphidium griffithii
Phacus agilis
Phaeocystis globosa
Staurastrum inconspicuum
Fragilaria capucina
Tetraëdron incus
Treubaria triappendiculata
Uroglena americana
Volvox aureus
Volvox carteri
Volvox carteri
Tetrahymena pyriformis
Amphidinium britannicum
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Actinastrum hantzschii
Aphanocapsa montana
Asterionella glacialis
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Cachonina niei

421
422
423
424
425
426
427
428
429
430
431
432
433
434
436
437
438
439
440
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
471
472
473
474
475
476
477

Carteria crucifera
Carteria inversa
Carteria inversa
Carteria cerasiformis
Carteria cerasiformis
Carteria klebsii
Carteria multifilis
Carteria obtusa
Carteria obtusa
Carteria obtusa
Carteria obtusa
Carteria radiosa
Chamaesiphon polymorphus
Chamaesiphon subglobosus
Characium polymorphum
Chlamydomonas fasciata
Chlamydomonas monadina var. monadina
Chlorogonium neglectum
Chlamydomonas parkeae
Chlamydomonas tetragama
Chloromonas insignis
Closterium acerosum
Closterium pleurodermatum
Closterium praelongum var. brevius
Closterium praelongum var. brevius
Cosmarium hians
Dictyosphaerium pulchellum
Draparnaldia plumosa
Errerella bornhemiensis
Eudorina elegans var. elegans
Eudorina elegans var. elegans
Eudorina elegans var. synoica
Eudorina illinoisensis
Eudorina illinoisensis
Eunotia pectinalis var. minor
Fibrocapsa japonica
Glenodiniopsis uliginosa
Gloeomonas lateperforata
Gomphonema gracile var. gracile
Gomphonema parvulum var. parvulum
Gomphonema parvulum var. parvulum
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Hemidinium nasutum
Heterocapsa pygmaea
Heterocapsa pygmaea
Lobomonas monstruosa
Mesostigma viride
Mesostigma viride
Mesostigma viride

478
479
480
481
483
484
485
486
487
488
489
494
495
497
499
501
503
504
505
506
507
509
510
512
514
515
519
520
522
523
524
527
528
529
530
531
532
533
534
536
537
538
539
540
541
542
543
544
545
546

- 202 -

Microcystis aeruginosa
Microthamnion kützingianum
Monoraphidium circinale
Myxosarcina burmensis
Nephroselmis olivacea
Nephroselmis olivacea
Nephroselmis olivacea
Nephroselmis viridis
Nitzschia palea
Nitzschia palea
Nitzschia palea
Oxyrrhis marina
Peridinium bipes f. globosum
Peridinium bipes f. occultatum
Peridinium inconspicuum subsp. remotum
Peridinium volzii
Phormidium foveolarum
Phormidium foveolarum
Phormidium foveolarum
Phormidium jenkelianum
Phormidium jenkelianum
Phormidium molle
Phormidium mucicola
Phormidium tenue
Planctonema lauterbornii
Plectonema radiosum
Alexandrium catenella
Alexandrium catenella
Pseudocarteria mucosa
Pseudocarteria mucosa
Pseudocarteria mucosa
Spirulina subsalsa
Staurastrum paradoxum
Stichococcus bacillaris
Stichococcus bacillaris
Stigeoclonium aestivale
Stigeoclonium fasciculare var. fasciculare
Tetraselmis cordiformis
Thalassionema nitzschioides
Ulothrix zonata
Ulothrix zonata
Uronema confervicolum
Uronema gigas
Uronema gigas
Volvox aureus var. aureus
Volvox aureus var. aureus
Volvox prolificus
Volvox tertius
Volvulina steinii
Volvulina steinii

547
548
553
556
557
558
559
560
561
562
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
592
593
594
595
596
597
598
600
601
603
604
605
608

Cyanophora paradoxa
Acinetospora crinita
Chaetoceros sociale
Triceratium dubium
Chattonella antiqua
Chattonella antiqua
Chattonella marina
Fibrocapsa japonica
Heterosigma akashiwo
Chrysochromulina parva
Astrephomene perforata
Astrephomene perforata
Basichlamys sacculifera
Characiochloris sasae
Eudorina elegans var. synoica
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Tetrabaena socialis var. socialis
Pandorina colemaniae
Pandorina colemaniae
Pandorina morum var. morum
Pandorina morum var. morum
Pleodorina californica
Pleodorina japonica
Yamagishiella unicocca
Yamagishiella unicocca
Volvox carteri f. kawasakiensis
Volvox carteri f. kawasakiensis
Volvulina compacta
Volvulina compacta
Volvulina steinii
Volvulina steinii
Chaetoceros didymus
Hantzschia amphioxys var. compacta
Lithodesmium variabile
Odontella aurita
Odontella longicruris
Fischerella major
Hydrococcus rivularis
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Spirulina platensis
Spirulina subsalsa
Peridinium bipes var. tabulatum
Prorocentrum micans
Chattonella ovata
Microcystis aeruginosa
Fibrocapsa japonica
Prorocentrum micans

609
610
611
612
613
614
615
617
618
619
620
621
622
623
624
626
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661

- 203 -

Pyrocystis lunura
Planktothrix rubescens
Phormidium tenue
Alexandrium hiranoi
Amphidinium klebsii
Cachonina niei
Coolia monotis
Prorocentrum lima
Prorocentrum mexicanum
Woloszynskia leopoliense
Gomphonema angustatum var. obtusatum
Botrydiopsis arrhiza
Botrydium granulatum
Pavlova gyrans
Chlorarachnion reptans
Pterosperma cristatum
Astrephomene gubernaculifera
Chlorella protothecoides
Carteria crucifera
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria eugametos
Characiochloris acuminata
Characiochloris sasae
Characium angustum
Chlorella saccharophila
Chlorella vulgaris var. vulgaris
Chlorella vulgaris var. vulgaris
Eremosphaera viridis
Eremosphaera viridis
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium viridistellatum
Gonium viridistellatum
Hafniomonas montana
Mesotaenium kramstae
Mesotaenium kramstae
Oocystis borgei
Oocystis lacustris
Oocystis lacustris662 Oocystis lacustris
663 Pleurotaenium nodosum var. borgei
664 Pleurotaenium nodosum var. borgei
665 Staurastrum dorcidentiferum
666 Yamagishiella unicocca
667 Yamagishiella unicocca
668 Paramecium bursaria
670 Chattonella verrculosa
671 Chattonella ovata
672 Oltmannsiellopsis geminata
674 Alexandrium catenella
675 Alexandrium catenella
677 Alexandrium catenella
678 Alexandrium insuetum
680 Gymnodinium mikimotoi
682 Prorocentrum dentatum
683 Prorocentrum sigmoides
684 Scrippsiella sweeneyae
685 Scenedesmus abundans
686 Chlorella vulgaris var. vulgaris
687 Graesiella emersonii
688 Graesiella emersonii
689 Graesiella emersonii
690 Graesiella emersonii
691 Tetrabaena socialis
692 Chlorogonium capillatum
693 Volvox aureus
694 Volvox aureus
695 Synura sphagnicola
696 Synura sphagnicola
697 Cryptomonas acuta
698 Cryptomonas irregularis
699 Rhodomonas atrorosea
700 Rhodomonas baltica
701 Rhodomonas chrysoidea
702 Rhodomonas falcata
703 Chroomonas collegionis
704 Chroomonas dispersa
705 Chroomonas placoidea
706 Chroomonas nordstedtii
707 Chroomonas nordstedtii
708 Chroomonas nordstedtii
709 Chroomonas nordstedtii
710 Chroomonas nordstedtii
711 Chroomonas nordstedtii
712 Chroomonas caudata
713 Chroomonas coerulea
714 Chroomonas coerulea
715 Chilomonas paramecium
716 Haramonas dimorpha

717 Eudorina elegans var. elegans
718 Eudorina elegans var. elegans
719 Eudorina elegans var. elegans
720 Eudorina elegans var. elegans
721 Eudorina elegans var. carteri
722 Eudorina cylindrica
723 Eudorina illinoisensis
724 Eudorina unicocca var. unicocca
725 Eudorina unicocca var. unicocca
726 Eudorina unicocca var. peripheralis
727 Paulschulzia pseudovolvox
728 Platydorina caudata729 Platydorina caudata
730 Volvox barberi
731 Volvox dissipatrix
732 Volvox carteri f. kawasakiensis
733 Volvox carteri f. kawasakiensis
734 Volvox rousseletii
735 Pleodorina californica
736 Pleodorina indica
737 Gonium multicoccum
738 Pteromonas aculeata
739 Pteromonas angulosa
740 Pteromonas multipyrenoidea
741 Chrysochromulina hirta
742 Chlorogonium capillatum
743 Chlorogonium capillatum
744 Chlorogonium capillatum
745 Chlorogonium capillatum
746 Chlorogonium capillatum
747 Chlorogonium capillatum
748 Chlorogonium capillatum
749 Chlorogonium capillatum
750 Chlorogonium capillatum
751 Chlorogonium elongatum
752 Chlorogonium elongatum
753 Chlorogonium elongatum
754 Chlorogonium euchlorum
755 Chlorogonium euchlorum
756 Chlorogonium euchlorum
757 Chlorogonium euchlorum
758 Chlorogonium euchlorum
759 Chlorogonium euchlorum
760 Chlorogonium euchlorum
761 Chlorogonium kasakii
762 Yamagishiella unicocca
763 Cyanophora paradoxa
764 Cyanophora tetracyanea
765 Rhodomonas duplex
766 Chilomonas paramecium- 204 -

767
768
769
770
771
772
773
774
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817

Chilomonas paramecium
Cosmarium askenasyi
Cosmarium askenasyi
Cosmarium askenasyi
Cosmarium askenasyi
Euastrum turgidum
Euastrum turgidum
Micrasterias anomala
Micrasterias anomala
Micrasterias foliacea
Micrasterias foliacea
Micrasterias mahabuleshwarensis
Micrasterias mahabuleshwarensis
Micrasterias thomasiana var. notata
Micrasterias thomasiana var. notata
Micrasterias truncata var. pusilla
Micrasterias truncata var. pusilla
Pleurotaenium nodosum var. nodosum
Pleurotaenium nodosum var. nodosum
Pleurotaenium nodosum var. gutwinskii
Pleurotaenium nodosum var. gutwinskii
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Cyclotella meneghiniana
Cyclotella meneghiniana
Cyclotella meneghiniana
Anabaena compacta
Anabaena kisseleviana
Anabaena mendotae
Anabaena mucosa
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica

818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867

- 205 -

Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena ucrainica
Anabaena ucrainica
Anabaena viguieri
Anabaena ellipsoides
Anabaena oumiana
Anabaena smithii
Anabaena smithii
Anabaena ucrainica
Anabaena lemmermannii
Anabaena planktonica
Anabaena compacta
Botryococcus braunii
Emiliania huxleyi
Gephyrocapsa oceanica
Cosmarium dilatatum
Euastrum biverrucosum
Staurastrum levanderi
Staurastrum tsukubicum
Microcystis aeruginosa
Planktothrix mougeotii
Planktothrix pseudagardhii
Tychonema bourrelii
Limnothrix redekei
Chattonella minima
Chattonella ovata
Chattonella verruculosa
Gonium octonarium
Gonium octonarium
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Eudorina minodii
Gonium viridistellatum
Phacotus lenticularis
Phacotus lenticularis
Pteromonas aculeata
Pteromonas angulosa
Pteromonas angulosa
Volvox africanus
Volvox aureus
Volvox carteri f. nagariensis
Volvox carteri f. weismannia
Volvox gigas

868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917

918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
966
967

Volvox obversus
Volvox tertius
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Vitreochlamys aulata
Vitreochlamys aulata
Vitreochlamys aulata
Vitreochlamys aulata
Vitreochlamys fluviatilis
Vitreochlamys gloeocystiformis
Vitreochlamys nekrassovii
Vitreochlamys ordinata
Vitreochlamys pinguis
Chlamydomonas debaryana var. cristata
Gonium multicoccum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Volvox aureus
Volvox aureus
Volvulina boldii
Volvulina boldii
Volvulina pringsheimii
Volvulina steinii
Volvulina steinii
Volvulina steinii
Ceratium fusus
Prorocentrum dentatum
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix pseudagardhii
Planktothrix pseudagardhii
Planktothrix pseudagardhii
Planktothricoides raciborskii- 206 -

Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothrix rubescens
Lyngbya hieronymusii var. hieronymusii
Cylindrospermopsis raciborskii
Gloeocapsa decorticans
Raphidiopsis curvata
Microcystis aeruginosa
Nephroselmis spinosa
Nephroselmis spinosa
Pterosperma cristatum
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Glaucocystis nostochinearum
Trentepohlia sp.

968 Chlamydomonas kuwadae
969 Synechococcus sp.
970 Synechococcus sp.
971 Synechococcus sp.
972 Synechococcus sp.
973 Synechococcus sp.
974 Synechococcus sp.
975 Synechococcus sp.
976 Synechococcus sp.
977 Synechococcus sp.
978 Synechococcus sp.
979 Synechococcus sp.
980 Synechococcus sp.
981 Synechococcus sp.
982 Synechococcus sp.
983 Synechococcus sp.
984 Synechococcus sp.
985 Synechococcus sp.
986 Synechococcus sp.
987 Synechococcus sp.
988 Synechococcus sp.
989 Planktothrix agardhii
990 Planktothrix agardhii
991 Cylindrospermopsis raciborskii
992 Cylindrospermopsis raciborskii
993 Cylindrospermopsis raciborskii
994 Cylindrospermopsis raciborskii
995 Mesostigma viride
996 Stichococcus ampulliformis
997 Calyptrosphaera sphaeroidea
998 Chrysochromulina quadrikonta
999 Emiliania huxleyi
1000 Gephyrocapsa oceanica
1001 Imantonia rotunda
1002 Glossomastix chrysoplasta
1003 Pelagomonas calceorata1004 Chroomonas coerulea
1005 Rhodomonas chrysoidea
1006 Rhodomonas salina
1007 Synura petersenii
1008 Pedinella squamata1009 Gonyostomum semen
1011 Ophiocytium capitatum
1012 Cafeteria roenbergensis
1013 Placidia cafeteriopsis
1014 Placidia cafeteriopsis
1015 Wobblia lunata
1016 Hymenomonas coronata
1017 Prymnesium parvum
1018 Prymnesium parvum

1019 Tetraselmis striata
1020 Apiocystis brauniana
1021 Chlamydomonas coccoides
1022 Chlamydomonas parkeae
1023 Spirogyra sp.
1025 Microcystis aeruginosa
1026 Microcystis aeruginosa
1027 Microcystis aeruginosa
1028 Microcystis aeruginosa
1029 Microcystis aeruginosa
1031 Chroogloeocystis siderophila
1032 Porphyridium sp.
1033 Porphyridium sp.
1034 Porphyridium sp.
1035 Porphyridium sp.
1036 Rhodella sp.
1037 Rhodella sp.
1038 Gonium multicoccum
1039 Gonium multicoccum
1040 Cylindrospermopsis raciborskii
1041 Cylindrospermopsis raciborskii
1042 Cylindrospermopsis raciborskii
1043 Microcystis aeruginosa
1044 Schizocladia ischiensis
1045 Cylindrotheca closterium
1046 Cylindrotheca fusiformis
1047 Cylindrotheca sp.
1048 Chlamydomonas noctigama
1049 Eucampia zodiacus
1050 Microcystis aeruginosa
1051 Microcystis aeruginosa
1052 Microcystis aeruginosa
1053 Microcystis aeruginosa
1054 Microcystis aeruginosa
1055 Microcystis aeruginosa
1056 Microcystis aeruginosa
1057 Microcystis aeruginosa
1058 Microcystis aeruginosa
1059 Microcystis aeruginosa
1060 Microcystis aeruginosa
1061 Microcystis aeruginosa
1062 Microcystis aeruginosa
1063 Microcystis aeruginosa
1064 Microcystis aeruginosa
1065 Microcystis aeruginosa
1066 Microcystis aeruginosa
1067 Microcystis aeruginosa
1068 Microcystis aeruginosa
1069 Microcystis aeruginosa
1070 Microcystis aeruginosa- 207 -

1071 Microcystis aeruginosa
1072 Microcystis aeruginosa
1073 Microcystis aeruginosa
1074 Microcystis aeruginosa
1075 Microcystis aeruginosa
1076 Microcystis aeruginosa
1077 Microcystis aeruginosa
1078 Microcystis aeruginosa
1079 Microcystis aeruginosa
1080 Microcystis aeruginosa
1081 Microcystis aeruginosa
1082 Microcystis aeruginosa1083 Microcystis aeruginosa1084 Microcystis aeruginosa
1085 Microcystis aeruginosa
1086 Microcystis aeruginosa
1087 Microcystis aeruginosa1088 Microcystis aeruginosa1089 Microcystis aeruginosa
1090 Microcystis aeruginosa
1091 Microcystis aeruginosa
1092 Microcystis aeruginosa
1093 Microcystis aeruginosa
1094 Microcystis aeruginosa
1095 Microcystis aeruginosa
1096 Microcystis aeruginosa
1097 Microcystis aeruginosa
1098 Microcystis aeruginosa
1099 Microcystis aeruginosa
1100 Microcystis aeruginosa
1101 Microcystis aeruginosa
1102 Microcystis aeruginosa
1103 Microcystis aeruginosa
1104 Microcystis aeruginosa
1105 Microcystis aeruginosa
1106 Microcystis aeruginosa
1107 Microcystis aeruginosa
1108 Microcystis aeruginosa
1109 Microcystis aeruginosa
1110 Microcystis aeruginosa
1111 Microcystis aeruginosa
1112 Microcystis aeruginosa
1113 Microcystis aeruginosa
1114 Microcystis aeruginosa
1115 Microcystis aeruginosa
1116 Microcystis aeruginosa
1117 Microcystis aeruginosa
1118 Microcystis aeruginosa
1119 Microcystis aeruginosa
1120 Microcystis aeruginosa

1121 Microcystis aeruginosa
1122 Microcystis aeruginosa
1123 Microcystis aeruginosa
1124 Microcystis aeruginosa
1125 Microcystis aeruginosa
1126 Microcystis aeruginosa
1127 Microcystis aeruginosa
1128 Microcystis aeruginosa
1129 Microcystis aeruginosa
1130 Microcystis aeruginosa
1131 Microcystis aeruginosa
1132 Microcystis aeruginosa
1133 Microcystis aeruginosa
1134 Microcystis aeruginosa
1135 Microcystis aeruginosa
1136 Microcystis aeruginosa
1137 Microcystis aeruginosa
1138 Microcystis aeruginosa
1139 Microcystis aeruginosa
1140 Microcystis aeruginosa
1141 Microcystis aeruginosa
1142 Microcystis aeruginosa
1143 Microcystis aeruginosa
1144 Microcystis aeruginosa
1145 Microcystis aeruginosa
1146 Microcystis aeruginosa
1147 Microcystis aeruginosa
1148 Microcystis aeruginosa
1149 Microcystis aeruginosa
1150 Microcystis aeruginosa
1151 Microcystis aeruginosa
1152 Microcystis aeruginosa
1153 Microcystis aeruginosa
1154 Microcystis aeruginosa
1155 Microcystis aeruginosa
1156 Microcystis aeruginosa
1157 Microcystis aeruginosa
1158 Microcystis aeruginosa
1159 Microcystis aeruginosa
1160 Microcystis aeruginosa
1161 Microcystis aeruginosa
1162 Microcystis aeruginosa
1163 Microcystis aeruginosa
1164 Microcystis aeruginosa
1165 Microcystis aeruginosa
1166 Microcystis aeruginosa
1167 Microcystis aeruginosa
1168 Microcystis aeruginosa
1169 Microcystis aeruginosa
1170 Microcystis aeruginosa- 208 -

1171 Microcystis aeruginosa
1172 Microcystis aeruginosa
1173 Microcystis aeruginosa
1174 Microcystis aeruginosa
1175 Microcystis aeruginosa
1176 Microcystis aeruginosa
1177 Microcystis aeruginosa
1178 Microcystis aeruginosa
1179 Microcystis aeruginosa
1180 Microcystis aeruginosa
1181 Microcystis aeruginosa
1182 Microcystis aeruginosa
1183 Microcystis aeruginosa
1184 Microcystis aeruginosa
1185 Microcystis aeruginosa
1186 Microcystis aeruginosa
1187 Microcystis aeruginosa
1188 Microcystis aeruginosa
1189 Microcystis aeruginosa
1190 Microcystis aeruginosa
1191 Microcystis aeruginosa
1192 Microcystis aeruginosa
1193 Microcystis aeruginosa
1194 Microcystis aeruginosa
1195 Microcystis aeruginosa
1196 Microcystis aeruginosa
1197 Microcystis aeruginosa
1198 Microcystis aeruginosa
1199 Microcystis aeruginosa
1200 Microcystis aeruginosa
1201 Microcystis aeruginosa
1202 Microcystis aeruginosa
1203 Microcystis aeruginosa
1204 Microcystis aeruginosa
1205 Microcystis aeruginosa
1206 Microcystis aeruginosa
1207 Microcystis aeruginosa
1208 Microcystis aeruginosa
1209 Microcystis aeruginosa
1210 Microcystis aeruginosa
1211 Microcystis aeruginosa
1212 Microcystis aeruginosa
1213 Microcystis aeruginosa
1214 Microcystis aeruginosa
1215 Microcystis aeruginosa
1216 Microcystis aeruginosa
1217 Microcystis aeruginosa
1218 Microcystis aeruginosa
1219 Microcystis aeruginosa
1220 Microcystis aeruginosa

1221 Microcystis aeruginosa
1222 Microcystis aeruginosa
1223 Microcystis aeruginosa
1224 Microcystis aeruginosa
1225 Microcystis aeruginosa
1226 Microcystis aeruginosa
1227 Microcystis aeruginosa
1228 Microcystis aeruginosa
1229 Microcystis aeruginosa
1230 Microcystis aeruginosa
1231 Microcystis aeruginosa
1232 Microcystis aeruginosa
1233 Microcystis aeruginosa
1234 Microcystis aeruginosa
1235 Microcystis aeruginosa
1236 Microcystis aeruginosa
1237 Microcystis aeruginosa
1238 Microcystis aeruginosa
1239 Microcystis aeruginosa
1240 Microcystis aeruginosa
1241 Microcystis aeruginosa
1242 Microcystis aeruginosa
1243 Microcystis aeruginosa
1244 Microcystis aeruginosa
1245 Microcystis aeruginosa
1246 Microcystis aeruginosa
1247 Microcystis aeruginosa
1248 Microcystis aeruginosa
1249 Microcystis aeruginosa
1250 Microcystis aeruginosa
1251 Microcystis aeruginosa
1252 Microcystis aeruginosa
1253 Microcystis aeruginosa
1254 Microcystis aeruginosa
1255 Microcystis aeruginosa
1256 Microcystis aeruginosa
1257 Microcystis aeruginosa
1258 Aphanizomenon flos-aquae
1259 Cylindrospermopsis raciborskii
1260 Cylindrospermopsis raciborskii
1261 Cylindrospermopsis raciborskii
1262 Cylindrospermopsis raciborskii
1263 Planktothrix agardhii
1264 Planktothrix agardhii
1265 Planktothrix agardhii
1266 Planktothrix rubescens
1267 Planktothrix rubescens
1268 Amphidinium tetsudo
1269 Chlorella sp.
1270 Nanochlorum sp.

- 209 -

1271 Trebouxia anticipata
1272 Trebouxia anticipata
1273 Trebouxia anticipata
1274 Trebouxia arboricola
1275 Trebouxia arboricola
1276 Trebouxia arboricola
1277 Trebouxia arboricola
1278 Trebouxia corticola
1279 Trebouxia corticola
1280 Trebouxia corticola
1281 Trebouxia corticola
1282 Trebouxia corticola
1283 Trebouxia corticola
1284 Trebouxia corticola
1285 Trebouxia corticola
1286 Trebouxia corticola
1287 Trebouxia corticola
1288 Trebouxia corticola
1289 Trebouxia higginsiae
1290 Trebouxia higginsiae
1291 Trebouxia higginsiae
1292 Trebouxia higginsiae
1293 Trebouxia higginsiae
1294 Trebouxia higginsiae
1295 Trebouxia higginsiae
1296 Trebouxia higginsiae
1297 Trebouxia showmanii
1298 Asterochloris cf. glomerata
1299 Asterochloris cf. glomerata
1300 Asterochloris cf. glomerata
1301 Asterochloris cf. glomerata
1302 Glossomastix chrysoplasta
1303 Fibrocapsa japonica
1304 Calcidiscus leptoporus
1305 Calcidiscus leptoporus
1306 Calcidiscus leptoporus
1307 Calcidiscus leptoporus
1308 Calyptrosphaera sphaeroidea
1309 Calyptrosphaera sphaeroidea
1310 Emiliania hyxleyi
1311 Emiliania hyxleyi
1312 Emiliania hyxleyi
1313 Emiliania hyxleyi
1314 Emiliania hyxleyi
1315 Gephyrocapsa oceanica
1316 Gephyrocapsa oceanica
1317 Gephyrocapsa oceanica
1318 Gephyrocapsa oceanica
1319 Gephyrocapsa oceanica
1320 Oolithotus fragilis

1321 Oolithotus fragilis
1322 Oolithotus fragilis
1323 Umbilicosphaera sibogae var. foliosa
1324 Umbilicosphaera sibogae var. sibogae
1325 Thoracosphaera heimii
1326 Thoracosphaera heimii
1327 Cryptomonas rostratiformis
1328 Gephyrocapsa oceanica
1329 Gephyrocapsa oceanica
1330 Prymnesium calathiferum- 210 -

2. Systematic index

ALGAE
Anabaena ucrainica
Anabaena ucrainica
Anabaena ucrainica
Anabaena variabilis
Anabaena viguieri
Anabaenopsis circularis
Aphanizomenon flos-aquae
Aphanizomenon flos-aquae f. gracile
Aphanocapsa montana
Aulosira laxa
Calothrix brevissima
Calothrix crustacea
Calothrix parasitica
Calothrix parasitica
Calothrix scopulorum
Chamaesiphon polymorphus
Chamaesiphon subglobosus
Chroogloeocystis siderophila
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Cylindrospermopsis raciborskii
Fischerella major
Gloeocapsa decorticans
Hydrococcus rivularis
Limnothrix redekei
Lyngbya hieronymusii var. hieronymusii
Merismopedia tenuissima
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa

CYANOPHYTA (CYANOBACTERIA)
Cyanophyceae
Anabaena affinis
Anabaena circinalis
Anabaena compacta
Anabaena compacta
Anabaena cylindrica
Anabaena ellipsoides
Anabaena flos-aquae f. flos-aquae
Anabaena flos-aquae f. flos-aquae
Anabaena flos-aquae f. flos-aquae
Anabaena kisseleviana
Anabaena lemmermannii
Anabaena mendotae
Anabaena mucosa
Anabaena oumiana
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena planktonica
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena smithii
Anabaena solitaria f. solitaria
Anabaena spiroides
Anabaena spiroides f. crassa
Anabaena spiroides f. spiroides
Anabaena spiroides f. spiroides
Anabaena spiroides f. spiroides

40
41
806
835
19
828
73
74
75
807
833
808
809
829
810
811
812
813
814
815
816
817
834
818
819
820
821
822
823
824
830
831
80
76
78
77
79
263

- 211 -

825
826
832
23
827
21
1258
81
416
50
22
266
267
334
268
433
434
1031
930
991
992
993
994
1040
1041
1042
1259
1260
1261
1262
592
931
593
847
929
230
44
87
88
89
90
91
98
99

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa

100
101
102
103
104
105
106
107
108
109
110
111
112
298
299
478
604
843
901
902
903
904
933
1025
1026
1027
1028
1029
1043
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1069
1070

- 212 -

1071
1072
1073
1074
1075
1076
1077
1078
1079
1080
1081
1082
1083
1084
1085
1086
1087
1088
1089
1090
1091
1092
1093
1094
1095
1096
1097
1098
1099
1100
1101
1102
1103
1104
1105
1106
1107
1108
1109
1110
1111
1112
1113
1114
1115
1116
1117
1118
1119
1120

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa

1121
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
1139
1140
1141
1142
1143
1144
1145
1146
1147
1148
1149
1150
1151
1152
1153
1154
1155
1156
1157
1158
1159
1160
1161
1162
1163
1164
1165
1166
1167
1168
1169
1170

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa- 213 -

1171
1172
1173
1174
1175
1176
1177
1178
1179
1180
1181
1182
1183
1184
1185
1186
1187
1188
1189
1190
1191
1192
1193
1194
1195
1196
1197
1198
1199
1200
1201
1202
1203
1204
1205
1206
1207
1208
1209
1210
1211
1212
1213
1214
1215
1216
1217
1218
1219
1220

Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Microcystis aeruginosa
Myxosarcina burmensis
Nostoc commune
Nostoc commune
Nostoc linckia
Nostoc linckia var. arvense
Nostoc minutum
Nostoc minutum
Oscillatoria amphibia
Oscillatoria animalis
Oscillatoria laetevirens
Oscillatoria limnetica
Oscillatoria rosea
Oscillatoria tenuis

1221
1222
1223
1224
1225
1226
1227
1228
1229
1230
1231
1232
1233
1234
1235
1236
1237
1238
1239
1240
1241
1242
1243
1244
1245
1246
1247
1248
1249
1250
1251
1252
1253
1254
1255
1256
1257
481
24
38
25
28
26
29
361
206
31
36
208
33

Phormidium foveolarum
Phormidium foveolarum
Phormidium foveolarum
Phormidium foveolarum
Phormidium foveolarum
Phormidium jenkelianum
Phormidium jenkelianum
Phormidium molle
Phormidium mucicola
Phormidium ramosum
Phormidium tenue
Phormidium tenue
Phormidium tenue
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothricoides raciborskii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix agardhii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix mougeotii
Planktothrix pseudagardhii
Planktothrix pseudagardhii
Planktothrix pseudagardhii
Planktothrix pseudagardhii
Planktothrix rubescens- 214 -

32
34
503
504
505
506
507
509
510
305
30
512
611
207
917
918
919
920
921
922
923
924
925
926
927
204
205
594
595
596
905
906
907
908
909
910
989
990
1263
1264
1265
844
911
912
913
845
914
915
916
610

Planktothrix rubescens
Planktothrix rubescens
Planktothrix rubescens
Plectonema radiosum
Raphidiopsis curvata
Spirulina platensis
Spirulina platensis
Spirulina platensis
Spirulina platensis
Spirulina subsalsa
Spirulina subsalsa
Spirulina subsalsa
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.

928
1266
1267
515
932
39
45
46
597
27
527
598
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
969
970
971
972
973
974
975
976
977

Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Synechococcus sp.
Tolypothrix tenuis
Tychonema bourrelii

978
979
980
981
982
983
984
985
986
987
988
37
846

GLAUCOPHYTA
Glaucophyceae
Cyanophora paradoxa
Cyanophora paradoxa
Cyanophora tetracyanea
Glaucocystis nostochinearum

547
763
764
966

RHODOPHYTA
Rhodophyceae
Porphyridium sp.
Porphyridium sp.
Porphyridium sp.
Porphyridium sp.
Rhodella sp.
Rhodella sp.

1032
1033
1034
1035
1036
1037

CRYPTOPHYTA
Cryptophyceae
Chilomonas paramecium
Chilomonas paramecium
Chilomonas paramecium
Chroomonas caudata
Chroomonas coerulea
Chroomonas coerulea
Chroomonas coerulea
Chroomonas collegionis- 215 -

715
766
767
712
713
714
1004
703

Chroomonas dispersa
Chroomonas nordstedtii
Chroomonas nordstedtii
Chroomonas nordstedtii
Chroomonas nordstedtii
Chroomonas nordstedtii
Chroomonas nordstedtii
Chroomonas placoidea
Cryptomonas acuta
Cryptomonas irregularis
Cryptomonas ovata
Cryptomonas ovata
Cryptomonas platyuris
Cryptomonas platyuris
Cryptomonas rostratiformis
Cryptomonas rostratiformis
Cryptomonas rostratiformis
Cryptomonas rostratiformis
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Cryptomonas tetrapyrenoidosa
Rhodomonas atrorosea
Rhodomonas baltica
Rhodomonas chrysoidea
Rhodomonas chrysoidea
Rhodomonas duplex
Rhodomonas falcata
Rhodomonas salina

704
706
707
708
709
710
711
705
697
698
274
275
276
344
277
278
345
1327
279
280
281
282
346
347
348
699
700
701
1005
765
702
1006

Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella antiqua
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella marina
Chattonella minima
Chattonella ovata
Chattonella ovata
Chattonella ovata
Chattonella verrculosa
Chattonella verrculosa
Fibrocapsa japonica
Fibrocapsa japonica
Fibrocapsa japonica
Fibrocapsa japonica
Fibrocapsa japonica
Gonyostomum semen
Haramonas dimorpha
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Heterosigma akashiwo
Olisthodiscus luteus

HETEROKONTOPHYTA
Chrysophyceae
Dinobryon divergens
Synura petersenii
Synura petersenii
Synura sphagnicola
Synura sphagnicola
Synura spinosa
Uroglena americana

284
233
1007
695
696
234
395

Dictyochophyceae
Pedinella squamata

Raphidophyceae
Chattonella antiqua

2
83
84
85
86
113
114
161
557
558
3
14
115
116
117
118
121
559
848
603
671
849
670
850
136
462
560
605
1303
1009
716
4
5
6
9
10
145
146
293
561
15

1

- 216 -

1008

Bacillariophyceae
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima
Achnanthes minutissima var. saprophila
Achnanthes subconstricta
Asterionella glacialis
Asterionella glacialis
Chaetoceros didymus
Chaetoceros sociale
Chaetoceros sociale
Cyclotella meneghiniana
Cyclotella meneghiniana
Cyclotella meneghiniana
Cylindrotheca closterium
Cylindrotheca fusiformis
Cylindrotheca sp.
Ditylum brightwellii
Eucampia zodiacus
Eunotia pectinalis var. minor
Fragilaria capucina
Gomphonema angustatum var. obtusatum
Gomphonema gracile var. gracile
Gomphonema parvulum var. parvulum
Gomphonema parvulum var. parvulum
Hantzschia amphioxys var. compacta
Lithodesmium variabile
Melosira granulata
var. angustissima f. spiralis
Nitzschia palea
Nitzschia palea
Nitzschia palea
Odontella aurita
Odontella longicruris
Skeletonema costatum
Skeletonema costatum
Skeletonema costatum
Skeletonema costatum
Skeletonema costatum
Tabellaria flocculosa
Thalassionema nitzschioides
Triceratium dubium

Phaeophyceae
71
407
408
409
410
411
412
413
414
372
330
265
417
586
377
553
803
804
805
1045
1046
1047
350
1049
461
391
620
465
466
467
587
588
333

Acinetospora crinita

548

Xanthophyceae
Botrydiopsis arrhiza
Botrydium granulatum
Ophiocytium capitatum

621
622
1011

Pelagophyceae
Pelagomonas calceorata

1003

Pinguiophyceae
Glossomastix chrysoplasta
Glossomastix chrysoplasta

1002
1302

Schizocladiophyceae
Schizocladia ischiensis

1044

HAPTOPHYTA
Prymnesiophyceae
Calcidiscus leptoporus
Calcidiscus leptoporus
Calcidiscus leptoporus
Calcidiscus leptoporus
Calyptrosphaera sphaeroidea
Calyptrosphaera sphaeroidea
Calyptrosphaera sphaeroidea
Chrysochromulina hirta
Chrysochromulina parva
Chrysochromulina quadrikonta
Cricosphaera roscoffensis
Emiliania huxleyi
Emiliania huxleyi
Emiliania hyxleyi
Emiliania hyxleyi
Emiliania hyxleyi
Emiliania hyxleyi

487
488
489
589
590
16
17
223
323
324
225
534
556

- 217 -

1304
1305
1306
1307
997
1308
1309
741
562
998
8
837
999
1310
1311
1312
1313

Emiliania hyxleyi
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Gephyrocapsa oceanica
Hymenomonas coronata
Imantonia rotunda
Oolithotus fragilis
Oolithotus fragilis
Oolithotus fragilis
Phaeocystis globosa
Prymnesium calathiferum
Prymnesium parvum
Prymnesium parvum
Umbilicosphaera sibogae var. foliosa
Umbilicosphaera sibogae var. sibogae

1314
353
838
1000
1315
1316
1317
1318
1319
1328
1329
1016
1001
1320
1321
1322
388
1330
1017
1018
1323
1324

Coolia monotis
Glenodiniopsis uliginosa
Gymnodinium mikimotoi
Hemidinium nasutum
Heterocapsa pygmaea
Heterocapsa pygmaea
Heterocapsa triquetra
Heterocapsa triquetra
Katodinium rotundatum
Oxyrrhis marina
Peridinium bipes f. globosum
Peridinium bipes f. occultatum
Peridinium bipes var. tabulatum
Peridinium inconspicuum subsp. remotum
Peridinium volzii
Peridinium volzii
Peridinium willei
Peridinium willei
Prorocentrum dentatum
Prorocentrum dentatum
Prorocentrum gracile
Prorocentrum lima
Prorocentrum mexicanum
Prorocentrum micans
Prorocentrum micans
Prorocentrum micans
Prorocentrum micans
Prorocentrum micans
Prorocentrum minimum
Prorocentrum minimum
Prorocentrum sigmoides
Prorocentrum triestinum
Protoceratium reticulatum
Protoceratium reticulatum
Pyrocystis lunura
Pyrophacus steinii
Scrippsiella sweeneyae
Scrippsiella trochoidea
Thoracosphaera heimii
Thoracosphaera heimii
Woloszynskia leopoliense

Pavlovophyceae
Pavlova gyrans

623

DINOPHYTA
Dinophyceae
Alexandrium catenella
Alexandrium catenella
Alexandrium catenella
Alexandrium catenella
Alexandrium catenella
Alexandrium catenella
Alexandrium hiranoi
Alexandrium insuetum
Amphidinium britannicum
Amphidinium carterae
Amphidinium klebsii
Amphidinium tetsudo
Cachonina niei
Cachonina niei
Ceratium fusus
Ceratium hirundinella
Coolia monotis

220
519
520
674
675
677
612
678
405
331
613
1268
420
614
899
376
343

615
463
680
471
472
473
7
235
356
494
495
497
600
499
365
501
304
366
682
900
315
617
618
12
218
316
601
608
237
238
683
219
318
319
609
321
684
369
1325
1326
619

EUGLENOPHYTA
Euglenophyceae
Euglena clara
Euglena gracilis
Euglena gracilis- 218 -

253
47
48

Euglena gracilis var. bacillaris
Euglena mutabilis
Eutreptiella gymnastica
Phacus agilis

49
286
381
387

Ulvophyceae

624

Trebouxiophyceae

Oltmannsiellopsis geminata
Oltmannsiellopsis unicellularis
Oltmannsiellopsis viridis
Ulothrix variabilis
Ulothrix zonata
Ulothrix zonata

CHLORARACHNIOPHYTA

672
359
360
329
536
537

Chlorarachniophyceae
Chlorarachnion reptans

Actinastrum hantzschii
Asterochloris cf. glomerata
Asterochloris cf. glomerata
Asterochloris cf. glomerata
Asterochloris cf. glomerata
Chlorella protothecoides
Chlorella saccharophila
Chlorella vulgaris var. vulgaris
Chlorella vulgaris var. vulgaris
Chlorella vulgaris var. vulgaris
Chlorella vulgaris var. vulgaris
Chlorella sp.
Dictyochloropsis irregularis
Dictyosphaerium pulchellum
Eremosphaera gigas
Eremosphaera viridis
Eremosphaera viridis
Eremosphaera viridis
Errerella bornhemiensis
Lagerheimia ciliata
Micractinium pusillum
Microthamnion kützingianum
Nanochlorum sp.
Oocystis borgei
Oocystis lacustris
Oocystis lacustris
Oocystis lacustris
Stichococcus ampulliformis
Stichococcus bacillaris
Stichococcus bacillaris
Trebouxia anticipata
Trebouxia anticipata
Trebouxia anticipata
Trebouxia arboricola
Trebouxia arboricola
Trebouxia arboricola
Trebouxia arboricola
Trebouxia corticola

CHLOROPHYTA
Prasinophyceae
Mesostigma viride
Mesostigma viride
Mesostigma viride
Mesostigma viride
Mesostigma viride
Monomastix minuta
Monomastix minuta
Nephroselmis astigmatica
Nephroselmis olivacea
Nephroselmis olivacea
Nephroselmis olivacea
Nephroselmis spinosa
Nephroselmis spinosa
Nephroselmis viridis
Pterosperma cristatum
Pterosperma cristatum
Pterosperma cristatum
Pyramimonas aff. amylifera
Pyramimonas aff. amylifera
Pyramimonas parkeae
Tetraselmis cordiformis
Tetraselmis cordiformis
Tetraselmis striata

296
475
476
477
995
255
256
252
483
484
485
934
935
486
221
626
936
251
320
254
18
533
1019

Pedinophyceae
Pedinomonas minor

363- 219 -

415
1298
1299
1300
1301
629
640
227
641
642
686
1269
378
453
379
380
643
644
455
382
151
479
1270
659
660
661
662
996
529
530
1271
1272
1273
1274
1275
1276
1277
1278

Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia corticola
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia higginsiae
Trebouxia showmanii

1279
1280
1281
1282
1283
1284
1285
1286
1287
1288
1289
1290
1291
1292
1293
1294
1295
1296
1297

Carteria obtusa
Carteria obtusa
Carteria obtusa
Carteria radiosa
Characiochloris acuminata
Characiochloris sasae
Characiochloris sasae
Characium angustum
Characium maximum
Characium polymorphum
Chlamydomonas augustae var. ellipsoidea
Chlamydomonas coccoides
Chlamydomonas debaryana var. cristata
Chlamydomonas fasciata
Chlamydomonas kuwadae
Chlamydomonas monadina var. monadina
Chlamydomonas monticola
Chlamydomonas noctigama
Chlamydomonas parkeae
Chlamydomonas parkeae
Chlamydomonas pulsatilla
Chlamydomonas tetragama
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium capillatum
Chlorogonium elongatum
Chlorogonium elongatum
Chlorogonium elongatum
Chlorogonium euchlorum
Chlorogonium euchlorum
Chlorogonium euchlorum
Chlorogonium euchlorum
Chlorogonium euchlorum
Chlorogonium euchlorum
Chlorogonium euchlorum
Chlorogonium fusiforme
Chlorogonium kasakii
Chlorogonium neglectum
Chloromonas insignis
Chlorosarcinopsis caeca
Chlorosarcinopsis delicata
Coelastrum astroideum
Coelastrum astroideum

Chlorophyceae
Apiocystis brauniana
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Astrephomene gubernaculifera
Astrephomene perforata
Astrephomene perforata
Basichlamys sacculifera
Botryococcus braunii
Brachiomonas submarina
Carteria cerasiformis
Carteria cerasiformis
Carteria crucifera
Carteria crucifera
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria eugametos
Carteria inversa
Carteria inversa
Carteria klebsii
Carteria multifilis
Carteria obtusa

1020
418
419
628
853
854
855
564
565
566
836
375
424
425
421
630
631
632
633
634
635
636
422
423
426
427
428

- 220 -

429
430
431
432
637
567
638
639
154
436
158
1021
884
437
968
438
157
1048
440
1022
122
446
692
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
123
761
439
447
160
153
129
130

Coelastrum astroideum
Coelastrum astroideum
Coelastrum morus
Coelastrum proboscideum
Coelastrum reticulatum
Coelastrum reticulatum var. reticulatum
Dimorphococcus lunatus
Dimorphococcus lunatus
Draparnaldia plumosa
Echinosphaeridium nordstedtii
Eudorina cylindrica
Eudorina elegans
Eudorina elegans var. carteri
Eudorina elegans var. elegans
Eudorina elegans var. elegans
Eudorina elegans var. elegans
Eudorina elegans var. elegans
Eudorina elegans var. elegans
Eudorina elegans var. elegans
Eudorina elegans var. synoica
Eudorina elegans var. synoica
Eudorina illinoisensis
Eudorina illinoisensis
Eudorina illinoisensis
Eudorina minodii
Eudorina unicocca var. peripheralis
Eudorina unicocca var. unicocca
Eudorina unicocca var. unicocca
Gloeomonas lateperforata
Gonium multicoccum
Gonium multicoccum
Gonium multicoccum
Gonium multicoccum
Gonium octonarium
Gonium octonarium
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Gonium pectorale var. pectorale
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium quadratum
Gonium viridistellatum
Gonium viridistellatum

244
342
231
131
132
245
134
135
454
137
722
351
721
456
457
717
718
719
720
458
568
459
460
723
856
726
724
725
464
737
885
1038
1039
851
852
468
469
569
570
645
646
647
648
649
650
651
652
653
288
289

Gonium viridistellatum
Gonium viridistellatum
Gonium viridistellatum
Gonium viridistellatum
Graesiella emersonii
Graesiella emersonii
Graesiella emersonii
Graesiella emersonii
Graesiella emersonii
Haematococcus lacustris
Hafniomonas montana
Hafniomonas montana
Hydrodictyon reticulatum
Lobomonas monstruosa
Monoraphidium circinale
Monoraphidium contortum
Monoraphidium griffithii
Oedogonium obesum
Pandorina colemaniae
Pandorina colemaniae
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum
Pandorina morum var. morum
Pandorina morum var. morum
Paulschulzia pseudovolvox
Pediastrum angulosum var. angulosum
Pediastrum boryanum
Pediastrum boryanum
Pediastrum duplex
Pediastrum duplex var. duplex
Pediastrum duplex var. duplex
Pediastrum duplex var. gracillimum
Pediastrum duplex var. gracillimum
Pediastrum simplex
Pediastrum simplex
Pediastrum tetras
Phacotus lenticularis
Phacotus lenticularis
Planctonema lauterbornii
Platydorina caudata
Platydorina caudata
Pleodorina californica
Pleodorina californica
Pleodorina indica- 221 -

290
654
655
857
226
687
688
689
690
144
257
656
295
474
480
384
385
203
572
573
242
243
362
886
887
888
889
890
574
575
727
300
209
301
212
210
213
211
214
215
302
216
858
859
514
728
729
576
735
736

Pleodorina japonica
Polyedriopsis spinulosa
Pseudocarteria mucosa
Pseudocarteria mucosa
Pseudocarteria mucosa
Pseudokirchneriella subcapitata
Pseudopleurococcus printzii
var. longissimus
Pteromonas aculeata
Pteromonas aculeata
Pteromonas angulosa
Pteromonas angulosa
Pteromonas angulosa
Pteromonas multipyrenoidea
Scenedesmus abundans
Scenedesmus acuminatusvar. tetradesmoides
Scenedesmus acutus
Scenedesmus acutus
Scenedesmus acutus
Scenedesmus dimorphus
Scenedesmus dimorphus
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus gutwinskii var. heterospina
Scenedesmus quadricauda
Scenedesmus serratus
Schroederia setigera
Stigeoclonium aestivale
Stigeoclonium fasciculare
var. fasciculare
Tetrabaena socialis
Tetrabaena socialis var. socialis
Tetracystis chlorococcoides
Tetraëdron incus
Trentepohlia sp.
Treubaria triappendiculata
Urnella terrestris
Uronema confervicolum
Uronema gigas
Uronema gigas
Vitreochlamys aulata
Vitreochlamys aulata
Vitreochlamys aulata
Vitreochlamys aulata
Vitreochlamys fluviatilis
Vitreochlamys gloeocystiformis

577
232
522
523
524
35
159

Vitreochlamys nekrassovii
Vitreochlamys ordinata
Vitreochlamys pinguis
Volvox africanus
Volvox aureus
Volvox aureus
Volvox aureus
Volvox aureus
Volvox aureus
Volvox aureus
Volvox aureus
Volvox aureus var. aureus
Volvox aureus var. aureus
Volvox barberi
Volvox carteri
Volvox carteri
Volvox carteri f. kawasakiensis
Volvox carteri f. kawasakiensis
Volvox carteri f. kawasakiensis
Volvox carteri f. kawasakiensis
Volvox carteri f. nagariensis
Volvox carteri f. weismannia
Volvox dissipatrix
Volvox gigas
Volvox obversus
Volvox prolificus
Volvox rousseletii
Volvox tertius
Volvox tertius
Volvulina boldii
Volvulina boldii
Volvulina compacta
Volvulina compacta
Volvulina pringsheimii
Volvulina steinii
Volvulina steinii
Volvulina steinii
Volvulina steinii
Volvulina steinii
Volvulina steinii
Volvulina steinii
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca
Yamagishiella unicocca

738
860
739
861
862
740
685
92
94
95
120
93
119
797
798
799
800
801
802
96
97
246
531
532
691
571
155
392
967
394
156
538
539
540
875
876
877
878
879
880

- 222 -

881
882
883
863
241
396
693
694
864
891
892
541
542
730
397
398
580
581
732
733
865
866
731
867
868
543
734
544
869
893
894
582
583
895
545
546
584
585
896
897
898
578
579
666
667
762
870
871
872
873

Yamagishiella unicocca874

Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium pleurodermatum
Closterium praelongum var. brevius
Closterium praelongum var. brevius
Closterium pusillum var. maius
Closterium rostratum var. subrostratum
Closterium selenastrum
Closterium selenastrum
Closterium spinosporum var. crassum
Closterium spinosporum var. crassum
Closterium spinosporum var. crassum
Closterium spinosporum var. malaysiense
Closterium spinosporum var. malaysiense
Closterium spinosporum var. ryukyuense
Closterium spinosporum var. ryukyuense

Charophyceae
Closterium acerosum
Closterium acerosum
Closterium acerosum
Closterium acerosum
Closterium aciculare var. subpronum
Closterium aciculare var. subpronum
Closterium calosporum var. calosporum
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. galiciense
Closterium calosporum var. himalayense
Closterium calosporum var. himalayense
Closterium calosporum var. himalayense
Closterium calosporum var. himalayense
Closterium ehrenbergii
Closterium ehrenbergii
Closterium gracile
Closterium gracile
Closterium incurvum
Closterium incurvum
Closterium moniliferum var. moniliferum
Closterium moniliferum var. moniliferum
Closterium moniliferum var. moniliferum
Closterium moniliferum
var. submoniliferum
Closterium moniliferum
var. submoniliferum
Closterium navicula
Closterium navicula
Closterium navicula
Closterium navicula
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex
Closterium peracerosumstrigosum-littorale complex

124
125
127
448
258
259
271
128
162
163
164
165
166
167
168
169
170
171
336
228
229
179
180
181
337
172
173
174
182
183
175
176
177
178
51
52
53
54

- 223 -

55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
261
262
449
450
451
185
338
339
340
186
187
341
188
189
191
192

Closterium spinosporum var. ryukyuense
Closterium spinosporum var. spinosporum
Closterium spinosporum var. spinosporum
Closterium spinosporum var. spinosporum
Closterium spinosporum var. spinosporum
Closterium tumidum
Closterium venus
Closterium wallichii
Closterium wallichii
Closterium wallichii
Cosmarium askenasyi
Cosmarium askenasyi
Cosmarium askenasyi
Cosmarium askenasyi
Cosmarium contractum
Cosmarium dilatatum
Cosmarium hians
Cosmocladium constrictum
Cylindrocystis brebissonii var. brebissonii
Docidium undulatum var. undulatum
Euastrum biverrucosum
Euastrum turgidum
Euastrum turgidum
Gonatozygon brebissonii
Gonatozygon brebissonii
Gonatozygon monotaenium
Gonatozygon monotaenium
Hyalotheca dissiliens
Hyalotheca dissiliens
Hyalotheca dissiliens
Hyalotheca dissiliens
Hyalotheca dissiliens
var. dissiliens f. tridentula
Mesotaenium kramstae
Mesotaenium kramstae
Micrasterias anomala
Micrasterias anomala
Micrasterias crux-melitensis
Micrasterias foliacea
Micrasterias foliacea
Micrasterias foliacea var. foliacea
Micrasterias mahabuleshwarensis
Micrasterias mahabuleshwarensis
Micrasterias thomasiana var. notata
Micrasterias thomasiana var. notata
Micrasterias truncata var. pusilla
Micrasterias truncata var. pusillaPenium margaritaceum
Penium margaritaceum

193
194
195
196
197
198
199
200
201
202
768
769
770
771
133
839
452
248
349
285
840
772
773
138
139
247
287
147
148
149
150
294
657
658
774
776
152
777
778
297
779
780
781
782
783
784
217
303

Pleurotaenium cylindricum
var. stuhlmannii
Pleurotaenium ehrenbergii var. curtum
Pleurotaenium ehrenbergii var. curtum
Pleurotaenium ehrenbergii var. curtum
Pleurotaenium ehrenbergii
var. ehrenbergii
Pleurotaenium ehrenbergii
var. ehrenbergii
Pleurotaenium nodosum var. borgei
Pleurotaenium nodosum var. borgei
Pleurotaenium nodosum var. gutwinskii
Pleurotaenium nodosum var. gutwinskii
Pleurotaenium nodosum var. nodosum
Pleurotaenium nodosum var. nodosum
Pleurotaenium nodosum var. nodosum
Pleurotaenium ovatum
Spinoclosterium cuspidatum
Spirogyra sp.
Staurastrum dejectum
Staurastrum dorcidentiferum
Staurastrum inconspicuum
Staurastrum levanderi
Staurastrum paradoxum
Staurastrum tsukubicum
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile
Triploceras gracile＊- 224 -

306
307
308
311
309
310
663
664
787
788
312
785
786
313
325
1023
224
665
390
841
528
842
789
790
791
792
793
794
795
796

The order of algal phyla and classes, and assignment ofstrains to each taxon in this index is according to thesystem in Chihara (Ed.), “Diversity and Evolution of
Algae” (Shokabo, Tokyo, 1999), and Nakayama, T.
(unpubl.).

PROTOZOA
Oligohymenophorea *Paramecium bursaria
Tetrahymena pyriformis

668
403

* See Ref.226.
Bicoecea
Cafeteria roenbergensis

1012

Placididea
Placidia cafeteriopsis
Placidia cafeteriopsis
Wobblia lunata

1013
1014
1015

- 225 -

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Fujita, Y. & Hattori, A. 1960 Formation ofphycoerythrin in pre-illuminated cells ofTolypothrix tenuis with special reference tonitrogen metabolism. Plant & Cell Physiol., 1,
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Fujita, Y. & Hattori, A. 1960 Effect ofchromatic lights on phycobilin formation in ablue-green alga, Tolypothrix tenuis. Plant &Cell Physiol., 1, 293-303.

30

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143, Prevention and Extermination Strategies ofHarmful Algal Blooms [Suisan-gaku shirîzu 134,Yûgai-yûdoku sôrui burûmu no yobô to kujo] ,Eds. by Hiroishi, S., Imai, I. & Ishimaru, T.,
Koseisha-Koseikaku, Tokyo, p. 43-53 (inJapanese without English title).

579

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& Showa Denko K.K. 1992 Separation andpurification of useful substances from Euglena.
[Euglena kara no yûyô-busshitsu no Bunri
Seisei.] In The Advanced Technology of the
Separation and Purification of the Materials for
Physiologically Functional Foods and Their

Development [Kinôsei Shokuhin Sozai no Kôdo
Bunri Seisei to Kaihatsu], Ed. and published by
The Japanese Research and Development
Association for the High Separation System inFood Industry [Shokuhin Sangyô Hai-separêshon
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- 256 -

Committee for Evaluating Microbial Culture Strains

Makoto M. Watanabe, D. Sci.

Environmental Biology Division

Kunimitsu Kaya, D. A.

Laboratory of Intellectual Fundamentals<Chairman>

for Environmental Studies
Yuhei Inamori, D. Sci.

Research Center for Material Cycles and Waste Management

Noriko Takamura, Ph. D

Regional and Community Environment Division

Akio Imai, Ph. D

Water and Soil Environment Division

Tomoharu Sano, D. Pharm.

Laboratory of Intellectual Fundamentals
for Environmental Studies

Junko Shimura, Ph. D

Laboratory of Intellectual Fundamentals
for Environmental Studies

Fumie Kasai, D. Sci.

Environmental Biology Division<Secretary>

Masanobu Kawachi, D. Sci.

Environmental Biology Division

Tomomichi Yanagita, D. Sci.

Professor Emeritus of University of Tokyo<Adviser>

Mitsuo Chihara, D. Sci.

Professor Emeritus of the University of Tsukuba<Adviser>Kazuo Komagata, D. A.

Professor Emeritus of University of Tokyo<Adviser>

Yoshinobu Shigenaka, D. Sci.

Professor Emeritus of Hiroshima University<Adviser>

Yuzaburo Ishida, D. A.

Professor of Fukuyama University<Adviser>

Isao Inouye, D. Sci.

Professor of the University of Tsukuba<Adviser>

Contributors
Fumie Kasai, D. Sci.
Masanobu Kawachi, D. Sci.
Mayumi Erata, D. Sci.
Makoto M. Watanabe, D. Sci.
Akira Shimizu
Fumi Mori
Kosei Yumoto
Mayumi Moriya, D. Sci.
Miwa Ishimoto

Environmental Biology Division
Environmental Biology Division
Global Environmental Forum
Environmental Biology Division
Social and Environmental Systems Division
Global Environmental Forum
Global Environmental Forum
Global Environmental Forum
Global Environmental Forum- 257 -

January 2004
Accepted by Editorial Board
National Institute for Environmental Studies[Research Report from the National Institute for Environmental Studies R-182-2004]
ISSN 1341-3643© National Institute for Environmental Studies 2004

NIES-Collection. List of Strains
Seventh Edition
Microalgae and Protozoa
2004
Correspondence
Fumie Kasai
Laboratory of Intellectual Fundamentals for Environmental Studies
National Institute for Environmental Studies

1 March, 2004
Published by National Institute for Environmental Studies
16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan,
Tel: +81-29-850-2556

Fax: +81-29-850-2587

http://www.nies.go.jp/biology/mcc/home.htm
Email: mcc@nies.go.jp

Printed by Nissei Eblo Co., Ltd.
Address: 3-13-11, Azuma, Tsukuba, Ibaraki 305-0031 Japan

Cover Photos

Front Cover

1

Gymnodinium mikimotoi (NIES-680)

2

Skeletonema costatum (NIES-16)

3

Spirulina subsalsa (NIES-27)

4

Tetrahymena pyriformis (NIES-403)

5

Botryococcus braunii (NIES-836)

6

Chattonella marina (NIES-3)

7

Porphyridium sp. (NIES-1035)

8

Volvox aureus var. aureus (NIES-541)

1

4

7

5

2

8

6

3

Back Cover
9

Actinastrum hantzschii (NIES-415)

10

Achnanthes subconstricta (NIES-330)

11

Acinetospora crinita (NIES-548)

12

Aulosira laxa (NIES-50)

13

Pediastrum duplex var. gracillimum (NIES-211)

14

Pleurotaenium ehrenbergii var. curtum (NIES-307)

15 Scenedesmus quadricauda (NIES-96)
16

Ceratium hirundinella (NIES-376)

17

Coelastrum astroideum (NIES-130)

13
9

11
12

10

16
14
15

17