For research purposes only, not for human use

Product Data Sheet
Anti-TRK B Antibody
Catalog #Source

Reactivity

Applications

CPA1832

RabbitH, M, R, B, C, D, Mk, Rb

WB, IH, IF/IC

Description

Rabbit polyclonal antibody to TRK B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the centerregion of human TRK B. The exact sequence is proprietary.

Purification

The antibody was purified by immunogen affinity chromatography.

Specificity

Recognizes endogenous levels of TRK B protein.

Clonality

Polyclonal

Conjugation
Form

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol,and 0.01% sodium azide.

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)

Gene Symbol

NTRK2

Alternative Names

TRKB; BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine
kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B

Entrez Gene

4915 (Human); 18212 (Mouse); 25054 (Rat)

SwissProt

Q16620 (Human); P15209 (Mouse); Q63604 (Rat)

Storage/Stability

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid
freeze/thaw cycles.

Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, ICImmunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility
Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference
Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, RbRabbit, S- Sheep, Z- Zebrafish

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For research purposes only, not for human use

Product Data Sheet
Western blot analysis of TRK B expression in rat heart (A),mouse kidney (B) whole cell lysates. (Predicted band size: 91
kD; Observed band size: 92; 130 kD)

Immunohistochemical analysis of TRK B staining in humanbrain formalin fixed paraffin embedded tissue section. Thesection was pre-treated using heat mediated antigen retrieval
with sodium citrate buffer (pH 6.0). The section was thenincubated with the antibody at room temperature anddetected using an HRP conjugated compact polymer system.
DAB was used as the chromogen. The section was thencounterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of TRK B staining in HL60 cells.
Formalin-fixed cells were permeabilized with 0.1% Triton
X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS
for 30 minutes at room temperature. Cells were probed withthe primary antibody in 3% BSA-PBS and incubated overnightat 4 °C in a humidified chamber. Cells were washed with PBST
and incubated with a DyLight 594-conjugated secondary
antibody (red) in PBS at room temperature in the dark. DAPI
was used to stain the cell nuclei (blue).

Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, ICImmunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility
Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference
Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, RbRabbit, S- Sheep, Z- Zebrafish

COHESION BIOSCIENCES LIMITEDWEB
www.cohesionbio.com

ORDER
order@cohesionbio.com

SUPPORT
techsupport@cohesionbio.com

CUSTOM
custom@cohesionbio.com