Kaneka Thermostatic Color Sensor

MyAbscope®
Instruction Manual
KN-T100901

MyAbscope® Instruction Manual

Introduction
Thank you for purchasing MyAbscope ® (Product code: KN-T100901, Product model:
PAS-822-KN). To ensure long-term use of this product, be sure to read this manual
carefully and fully understand the outline of this product, how to operate, and safety
precautions. No part of this manual may be copied to be used by a third party without
Kaneka’s permission.
The contents of this manual are subject to change without prior notice.
The design, specifications, and application for this product are subject to change without
prior notice.
The company names and product names described in this manual are trademarks orregistered trademarks of respective companies.

1

MyAbscope® Instruction ManualContents
Handling Precautions

3

Configuration

9

Operation Procedure10

1. Part Names of Main Unit

10

2. Operation Procedure (Preparation)

13

3. Operation Procedure (High-temperature Treatment Unit)

19

4. Operation Procedure (Thermostatic Absorbance Measurement Unit)

26

A. Setting reaction conditions

27

B. Light intensity calibration

32

C. Preheating

34

D. Thermostatic absorbance measurement

37

E. Finishing measurement and taking out sample

40

5. Data Analysis

42

Cleaning and Storage48

Troubleshooting50

After-sales Service

52

Contact

52

Specifications

53

Quality Assurance

54

2

MyAbscope® Instruction ManualHandling Precautions
To ensure your safety, read and understand the following handling precautions beforeuse.

WARNING

Indicates that a serious injury accident* may occur if you do notfollow the operating or maintenance procedure correctly.

* Severe injury accidents refer to loss of sight, injury, burns, electric shock, and others that haveaftereffects or require hospitalization or long-term medical treatment.

⚫

Do not disassemble this product or change the structure of this product.
Or it may cause electric shock, fuming, flaming, or failure.

⚫

Be sure to turn off the power of the main unit when performing maintenancesuch as cleaning.
Or it may cause electric shock.

⚫

If smoke or an unusual odor occurs, or water or foreign matter enters the mainunit, immediately turn OFF the main unit, disconnect the power plug from theoutlet, and stop using the product.
Or it may cause electric shock, fuming, flaming, or failure.

⚫

Do not directly touch the sample ports or sample tubes during and for a while
after measurement since they are hot.
Or it may cause burns.

⚫

Do not calibrate the light intensity with the heat bonnet open. Also, do not lookdirectly at the direct or reflected light of LED.
Or it may cause eye pain or blurred vision.

⚫

Use the AC/DC adapter supplied with this product. Make sure that the rated
power of the AC/DC adapter does not exceed the power supply capacity of theoutlet.
Or it may cause burns, fuming, flaming, or failure.

⚫

Do not cover the product with paper or cloth. Do not put anything on the main
unit. Be careful so that no liquids, metals, or flammable substances enter themain unit.
Or it may cause a fire.

⚫

Do not touch with wet hands.
Or it may cause electric shock, fuming, flaming, or failure.

⚫

Be careful not to scratch electric wires or insulated parts with a knife or the like.
Do not damage, alter, modify, forcibly bend, twist, stretch, apply heavy load to,or heat the power cable or connectors.
Using them in a damaged state may cause electric leakage, electric shock, or a fire.

3

MyAbscope® Instruction Manual⚫

Be careful when opening the heat bonnet while the product is in operation.
Since hot solution may scatter. Also, insert and remove sample tubes in a
sufficiently cooled state.
Or it may cause injury or burns.

4

MyAbscope® Instruction ManualHandling Precautions
To ensure your safety, read and understand the following handling precautions before
use.

CAUTION

Indicates that a minor injury accident* may occur if you do
not follow the operating or maintenance procedure correctly.

* Minor injury accidents refer to injury, burns, electric shock, and others that do not require hospitalization
or long-term medical treatment.

⚫

Do not place this product in direct sunlight.
Or it may cause fuming, flaming, or failure.

⚫

We recommend using this product in an environment with a temperature of 20
to 30°C and a humidity of 70% or less.
Using this product in a low-temperature or high-humidity environment may cause electricleakage or failure due to condensation, and using it in a high temperature environment may
cause fuming, flaming, or failure.

⚫

Do not use this product with the main unit exposed to water or oil, in ahigh-humidity, flammable or corrosive atmosphere, or in a dusty place.
Or it may cause failure, fuming, or flaming.

⚫

Do not place this product in unstable places, such as on a wobbling table, in an
inclined place, and in a place with vibration.
Or it may cause failure or injury.

⚫

If the main unit dropped, immediately turn it OFF and disconnect the powerplug from the outlet.
Or it may cause fuming, flaming, or injury.

⚫

Do not use the main unit if it dropped.
Or it may cause fuming, flaming, or injury.

⚫

Do not use the heat bonnet if it came off the main unit.
Or it may cause burns or injury.

⚫

Do not transport the main unit by holding the power cable or heat bonnet.
When moving or transporting the main unit, hold the handles on both sides
firmly.
Or it may cause failure, fuming, or flaming.

⚫

Remove the power plug and cables connected to external devices whenmoving or transporting the main unit.
Or it may cause failure, fuming, or flaming.

⚫

Do not block the air vents. Using this product with the air vents blocked may
cause failure, fuming, or flaming due to overheating.
Or it may cause failure, fuming, or flaming.

5

MyAbscope® Instruction Manual⚫

Be sure to turn OFF the power of the main unit after use. Even after the control
application screen is turned OFF, the main unit may still be in operation.
Or it may cause fuming, flaming, injury, or failure.

⚫

Be careful not to get your fingers caught in the heat bonnet.
Or it may cause injury or burns.

⚫

The periphery of the heat bonnet is hot while the heater is in operation. Do nottouch it.
Or it may cause injury or burns.

⚫

Exhaust air comes out from behind the heat bonnet while the cooling fan isoperating. Be careful of burns.
Or it may cause burns.

6

MyAbscope® Instruction ManualHandling Precautions
Please acknowledge the following precautions before using this product.
⚫

This product is for research use only and not designed to be applied to fields
requiring high reliability, such as medical care. Be aware that Kaneka will notbear any responsibility for any loss or damage caused by using this product insuch fields.

⚫

Use the main unit in an indoor place of horizontal and pollution level 2, altitudeof 2000 meters or less (overvoltage category II).

⚫

The sample volume should be up to 500 L/tube for the high-temperature
treatment unit, and 25 to 100 L /tube for the thermostatic absorbancemeasurement unit.

⚫

Do not prepare solution on or above the main unit. Prepare solution in anotherplace, securely close the caps of the tubes, then set the tubes to the main unit.

⚫

Do not move this product with the fan filters removed.

⚫

The high-temperature treatment unit and thermostatic absorbancemeasurement unit cannot be used simultaneously.

Safety⚫

Heating stops when the heater reaches a certain temperature.
High-temperature treatment unit

HeatLid*1: 130°C, Well*2: 120°C

Thermostatic absorbance measurement unit

HeatLid: 100°C, Well: 90°C

*1: Contact section with the tube cap inside the heat bonnet*2: Heating section of the tube

Light measurement⚫

Calibrate the light intensity for every measurement.

⚫

Since measurement results may vary depending on the type of tube, we do notrecommend using multiple types of tubes for comparative evaluation.

Cooling⚫

If the main unit unintentionally stops while this product is in operation, thecooling fan is not activated when the product is restarted. In this case, natural
cooling is required for the main unit.

Communication/control device⚫

The operation of the application is checked with Nexus 9 or ZenPad 3 8.0 (Z581KL) or
MediaPad M3 Lite 10 (hereinafter referred to as “tablet”).

⚫

If the application task is terminated without tapping the Stop button on the application
screen, the main unit may still be in operation. Be sure to turn OFF the power of themain unit.

⚫

If any of the following phenomena occurs during absorbance measurement, theongoing measurement cannot be resumed and data will be lost.
- Disconnection of Bluetooth connection

7

MyAbscope® Instruction Manual- Shutdown of the tablet- Suspension of power supply to the main unit⚫

Do not use this product near medical electrical equipment such as cardiac pacemakers.
Such equipment may be affected by radio waves.

⚫

This unit only supports Bluetooth Low Energy and does not support any other wirelessconnections.

⚫

This product uses the 2.4 GHz frequency band. This frequency band is used not only
by industrial, scientific, and medical equipment, but also by other similar wirelessstations, mobile object identifying premises radio stations requiring licenses, which are
typically used in factory manufacturing lines, specific low-power radio stations requiring
no licenses, amateur radio stations, and others (hereinafter referred to as “otherwireless stations”). Make sure that no “other wireless stations” are not in operation
nearby before using this product. If there is interference between this product and“other wireless stations,” immediately avoid radio wave interference by relocating thisproduct or turning OFF the power of the main unit.

8

MyAbscope® Instruction Manual

Configuration(1) Main unit ......................................................................................... 1 unit
(2) AC/DC adapter................................................................................ 1 piece(3) Instruction Manual ........................................................................... 1 copy(4) CD-ROM (containing application installation software) .................... 1 disk(5) MyAbscope® Application Installation Manual ................................... 1 copy

AC/DC adapter

Main unit

Do not disassemble this product or change the structure of this product.
（Or it may cause electric shock, fuming, flaming, or failure.）
Please confirm the shape and direction of the USB cable carefully and use itcorrectly. If you use something that does not fit in shape or size, there is a riskthat the device generates heat. （Or it may cause fuming, flaming.）
During measurement or after the measurement for a while, do not touch theright and left unit and sample tube directly. （Or it may cause burns.）
During heating, there is a possibility that the solution is bumping. Be careful ofburns. （Or it may cause injury or burns.）

Please read the instruction manual carefully before using this product.

9

MyAbscope® Instruction Manual

Operation Procedure
1. Part Names of Main Unit
<Main unit>
Heat bonnet

Thermostatic absorbancemeasurement unit
(for 0.2 mL tubes)

Figure 1-1: Top of main unit

<Sides of main unit>

HandleFigure 1-2: Sides of main unit

10

High-temperature
treatment unit
(for 1.5 mL tubes)

MyAbscope® Instruction Manual<Bottom of main unit>
Fan filter outlet

Product label

Bluetooth device address label
Figure 1-3: Bottom of main unit

<Back of main unit>
AC adapter
connection port

Air vent

microUSB
connection port

Bluetoothswitch*Air vent* Do not touch the Bluetooth switch (Left: OFF, Right: ON).
Turning it OFF disables communication with the control tablet.
Figure 1-4: Back of main unit

11

MyAbscope® Instruction Manual<Power button and pilot lamp on main unit>
Pilot lamp
Lights in red : The main unit is ON (Bluetooth not connected).
Lights in blue : Bluetooth connection has been established between the main unit
and tablet (Standby state).
Blinks in blue : The main unit is in operation (Light intensity calibration, heating, orabsorbance measurement).
Power button

Pilot lamp

Figure 1-5: Top of main unit

12

MyAbscope® Instruction Manual

2. Operation Procedure (Preparation)<Devices and accessories required for measurement>⚫

Main unit of MyAbscope⚫

AC/DC adapter (Type: B3, Accessory)⚫

Control tablet (Sold separately) * The dedicated application should be installed.

⚫

1.5 mL tubes (Sold separately)⚫

0.2 mL tubes (8-strip) (Sold separately. Recommended tube: Kaneka Microtube(0.2 mL 8-strip tubes with flat caps))<Starting main unit>(1) Connect the AC/DC adapter (19 V DC) to the main unit and outlet.
(2) Press the Power button on the main unit. The pilot lamp beside the Power buttonon the main unit will light in red.

<Starting software>(3) Start “MyAbscope” on the tablet. For how to install the application, see

MyAbscope® Application Installation Manual. Make sure that Bluetooth is ON on
the tablet.

Figure 2-1: MyAbscope application

When the MyAbscope application is activated, the connection setting screen(Figure 2-2) is displayed.

13

MyAbscope® Instruction Manual(1) Device Search button
Searches for aconnectable main unit.

(2) Connect button
Starts connection.

Figure 2-2: Connection setting screen<Connecting Bluetooth device>(4) Tap the “Device Search” button (1) to search for a connectable main unit. If no unit

is found (Figure 2-3), turn ON the power of the main unit again and tap the “Device
Search” button. If found, the Bluetooth device address is displayed as shown in
Figure 2-4.

(1)Figure 2-3: If not foundFigure 2-4: If found

14

MyAbscope® Instruction Manual(5) Tap the same address (Bluetooth device address) as that written on the bottom of

the main unit you intend to connect. The background color of the address changes(Figure 2-5) to indicate that it is selected.

(2)

Figure 2-5: Display after the address is selected(6) Tap the “Connect” button (2) to start connection.
(7) When the connection is established, a message “Connected.” is displayed on thescreen, then the top screen after connected is displayed. The pilot lamp beside thePower button on the main unit turns from red to blue, and the gray circle  on thelower right of the screen changes to a green circle  (Figure 2-6).

Figure 2-6: Top screen after connected

15

MyAbscope® Instruction Manual<View mode connection> (Used for checking the saved data only)
Start the main unit and software by following steps (1) to (3) above and tap “ViewMode” (3). Then the background color changes (Figure 2-7) to indicate that it isselected.

(3)

Figure 2-7: Display after View Mode is selected

Tap the “Connect” button to display the top screen after connected. For how to checkthe data, see “5. Data Analysis” procedure on page 42.

16

MyAbscope® Instruction Manual<Top screen>
Tap the “Connect” button (2) on the connection setting screen (Figure 2-2) to establish
the connection. Then the top screen (Figure 2-8) is displayed.
(4) High-temperature
treatment process button
Displays the screen for
performing high-temperature
treatment (Figure 3-4).

(5) Thermostatic
absorbancemeasurement process
button
Displays the screen for
performing thermostatic
absorbance measurement(Figure 4-2).

(6) Data button
Displays the screen forreferring the saved data
(Figure 5-2).

(7) Information display
button
Displays the screen for
performing thermostatic
absorbance measurement(Figure 4-2).

Home button
Returns to the home screen.

 In preparation
 In operation
 Not connected

Battery status of the main
unit
Battery voltage: Normal
Battery voltage: Low
Not connected with themain unit
Return button
Returns to the previous
screen.

Figure 2-8: Top screen

17

Application history button
Used for checking thehistory of the applications
used on the tablet.

MyAbscope® Instruction Manual<Information screen>
Firmware* version display
field
Displays the firmware
version information of themain unit connected.
Displays “N/A” when notconnected.
Bluetooth address display
field

Displays the Bluetooth
device address of themain unit connected.
Displays “View Mode” inview mode.
Application version
information display field
Displays the version
information of theapplication being used.

* Firmware refers to the software installed in the main unit.
Figure 2-9: Information screen (when a main unit is connected)

18

MyAbscope® Instruction Manual

3. Operation Procedure(High-temperature Treatment Unit)<Main unit>
High-temperature
treatment unit
(for 1.5 mL tubes)

Figure 3-1: High-temperature treatment unit (Right)

Applications: DNA extraction etc.

19

MyAbscope® Instruction Manual<Setting samples>(1) Slowly raise the heat bonnet.

Figure 3-2: Setting a tube to the high-temperature treatment unit of the main unit

(2) Set 1.5 mL tubes containing samples to the high-temperature treatment unit. At thattime, make sure that the caps of the 1.5 mL tubes are firmly closed (Figure 3-2).
(3) Slowly lower the heat bonnet and close it completely.
Handle the heat bonnet with care. It may be damaged by rough handling.

<Tablet operation>(4) Tap “High-temp. process,” the high-temperature treatment process button (Figure

3-3).

Figure 3-3: Top screen

20

MyAbscope® Instruction Manual

HeatLid*1 temperature, Well*2
temperature: Updated every
10 seconds (Not displayedwhen not connected with themain unit).
Time elapsed: Starts to be
counted when both the
HeatLid temperature andWell temperature haverespectively reached thepreset temperature ±1°C.

(1) File name input field(2) HeatLid temperature,
Well temperature, andheating time input fields(3) High-temperature
treatment start button
Valid only in the “Ready”
state.

(4) High-temperature
treatment stop button
Valid only in the “Heating”
state.

(5) Parameter file nameinput field

Not ready: Not ready for
operation
Ready: Ready for operation
Heating: In heating
Cooling: In cooling(6) Parameter file readbutton(7) Parameter file savebutton*1: Contact with the tube cap inside the heat bonnet*2: Heating section of the tube
Figure 3-4: High-temperature treatment process screen(5) Enter the CSV data storage file name in the file name input field (1).

If not entered, the CSV data storage file is automatically titled “untitled.”(6) Set the intended values for the HeatLid temperature, Well temperature, and heatingtime (2). When all parameters are specified, the status indication changes to“Ready” (Figure 3-5).
(Effective range of temperature and time for high-temperature treatment process)
HeatLid temperature: Up to one decimal place in the range from 35.0 to 120.0°C
Well temperature: Up to one decimal place in the range from 35.0 to 100.0°C
Heating time: Only integers from 1 to 10 minutes* If a value out of the above ranges is entered, an error message “Setting value is outof range.” is displayed.

21

MyAbscope® Instruction Manual

Figure 3-5: Indication of Ready state(How to save settings)
To save the settings, tap the parameter file save button (7) in Figure 3-4, enter the file name on the savescreen shown in Figure 3-6, then tap “Save.” If you tap “Cancel,” the file is not saved.
If a file with the same name exists, tap “Yes” on the confirmation screen shown in Figure 3-7 to overwrite it,or tap “No” to return to the parameter save screen.

Figure 3-6: Parameter save screen

Figure 3-7: Overwrite confirmation screen

To read the settings from saved parameters, highlight the parameter file name in (5) in Figure 3-4, then tap
the parameter file read button (6).

22

MyAbscope® Instruction Manual(7) Tap “Start,” the high-temperature treatment start button, (3) in Figure 3-4 to starthigh-temperature treatment process. When heating starts, the status indication
changes to “Heating,” the green circle  on the lower right of the tablet screenchanges to a red circle , and the pilot lamp beside the Power button on the main
unit blinks in blue.
(8) The HeatLid temperature and Well temperature are updated in real time every 10

seconds while being raised to the preset temperature and during high-temperature
treatment (Figure 3-8).

Figure 3-8: Indication of Heating state(9) After the preset time has elapsed, you will be informed that the data is saved asshown in Figure 3-9 on the next page. Then tap “OK.” The preset conditions are
saved in the CSV format (with the file name entered in the input field (1) on thehigh-temperature treatment process screen, which is suffixed with“_yymmddhhmmss.csv”* (see “5. Data Analysis,” (CSV file)). Three seconds after
the preset time has elapsed, the cooling fan starts to operate, and the statusindication changes to “Cooling” as shown in Figure 3-10. When both the HeatLid
temperature and Well temperature have fallen below 35°C (within about 10 minutes
or less), cooling is finished, and the status indication changes to “Ready” as shownin Figure 3-11. Confirm that the temperatures have fallen on the tablet screen.
* “yymmddhhmmss” stands for the time when the data is saved. For example, “160801133406” meansthat the data is saved at 13:34:06 on August 1, 2016.
Hot air may come out from the back of the heat bonnet. Be careful of burns.

23

MyAbscope® Instruction Manual

Figure 3-11: Indication of Ready state

Figure 3-9: Indication of Saved state

Figure 3-10: Indication of Cooling state(How to quit high-temperature treatment halfway)
To quit high-temperature treatment halfway, tap the high-temperature treatment stop button (4) in Figure 3-4.
You will be asked “Are you sure you want to stop?” as shown in Figure 3-12. Tap “Yes” to stop, or “No” tocontinue the high-temperature treatment.

Figure 3-12: High-temperature treatment stop confirmation screen
If you tap “Yes,” you will be informed that the data is saved as shown in Figure 3-9. Then tap “OK.” The
preset conditions are saved in the CSV format (with the file name entered in the input field (1) on thehigh-temperature process screen, which is suffixed with “_yymmddhhmmss.csv.” Three seconds after you
tap “Yes,” the cooling fan starts to operate and the status indication changes to “Cooling” as shown in Figure3-10. When both the HeatLid temperature and Well temperature have fallen below 35°C, the statusindication changes to “Ready” as shown in Figure 3-11.

24

MyAbscope® Instruction Manual<Taking out sample>

Figure 3-13: Taking out sample(10) Slowly raise the heat bonnet.
(11) Pull out the 1.5 mL tubes containing samples (Figure 3-13).
- Handle the heat bonnet with care. It may be damaged by rough handling.
- Tubes containing samples may be hot. Be careful of burns.

25

MyAbscope® Instruction Manual

4. Operation Procedure (Thermostatic
Absorbance Measurement Unit)<Thermostatic absorbance measurement flow>
A: Setting reaction conditions

B: Light intensity calibration

C: Preheating:: Light intensity calibration
D: Thermostatic absorbance measurement

E: Finishing measurement and taking out sample<Thermostatic absorbance measurement procedure>(1) Tap “Thermostatic absorbance meas. process” (Figure 4-1), the thermostatic

absorbance measurement process button, on the top screen after this product isconnected to display the thermostatic absorbance measurement process setting
screen (Figure 4-2).

Thermostatic absorbancemeasurement process button

Figure 4-1: Thermostatic absorbance measurement process button on top screen

26

MyAbscope® Instruction Manual[A: Setting reaction conditions](1) File name input field(2) Sensor selection button(3) Threshold input field(6) Parameter file savebutton(4) Parameter file nameinput field(7) Next button(5) Parameter file readbuttonFigure 4-2: Thermostatic absorbance measurement process setting screen(2) Enter the CSV data storage file name in the file name input field (1). If not entered,

the CSV data storage file is automatically titled “untitled.”

27

MyAbscope® Instruction Manual(3) Select the (R, G, or B) sensor suitable for the wavelength region to be measured

with the sensor selection buttons (2) in Figure 4-2. You can select only one sensor
for one measurement.
(Reference) The (typical) absorption wavelength band of each sensor is as follows:
R: 575 to 660 nm, G: 455 to 630 nm, B: 400 to 540 nm
For example, to measure a reaction system in which a colorless solution turns into
yellow, you should select the B sensor.
Color ofabsorbed
light

Absorbed
wavelength

Typical color of solution(Complementary color ofabsorbed light)

VioletYellow green

Dark
blue

Yellow

Blue

OrangeGreen

Red

Yellow

VioletOrangeBlue

RedBlue green

Absorbed
wavelengthband of
B sensor
Absorbed wavelengthband of G sensor
Absorbed
wavelengthband ofR sensor* The above colors are forillustrative purposes only.

Figure 4-3: Measurement wavelength band of each sensor and typical colors of sample solutions

It is not possible to set a specific wavelength.

(4) To set thresholds, select the absorbances to be used as the thresholds (then thevalues higher than “High” are displayed with “+,” those lower than “Low,” with “-,”and those between “High” and “Low,” with “±”). Otherwise, tap “Next.”(5) To set thresholds at the time of judgment, tap the threshold input field (3) in Figure

4-2 to display the numeric input screen as shown in Figure 4-4. Then select anumerical value for each channel and then tap “OK.” If you set a threshold for all, it
applies to all channels.

28

MyAbscope® Instruction Manual

Figure 4-4: Threshold setting screen(How to save parameters)
To save the parameters, tap the parameter file save button (6) in Figure 4-2, enter the file name on the savescreen shown in Figure 4-5, then tap “Save.” If you tap “Cancel,” the file is not saved.
If a file with the same name exists, tap “Yes” on the confirmation screen shown in Figure 4-6 to overwrite it,or tap “No” to return to the parameter save screen.

Figure 4-5: Parameter save screen

Figure 4-6: Overwrite confirmation screen

To read the saved parameters, select the parameter file name in (4) in Figure 4-2, then tap the parameter
file read button in (5).

29

MyAbscope® Instruction Manual(6) Tap “Next,” the next button, (7) in Figure 4-2 to display the thermostatic absorbancemeasurement process screen (Figure 4-7).
(2) HeatLid temperature, Welltemperature, and heating time
input fields for thermostatic
absorbance measurement

Displays the file name andsensor specified on thethermostatic absorbancemeasurement process setting
screen.

(3) HeatLid temperature, Welltemperature, and heating time
input fields for two-stepprocess(1) Delay Time input field(4) Light intensity calibration
start button
Valid only in the “Not ready”
and “Ready” states.

HeatLid temperature, Welltemperature: Updated every 10
seconds (Not displayed when notconnected with the main unit).
Time elapsed: Starts to be counted
when both the HeatLid temperatureand Well temperature haverespectively reached the preset
temperature ±1°C (for both reaction
and two-step processes).

(6) Reaction process start
buttonValid only in the “Preheating”
state.
Not ready: Not ready for operation
Ready: Ready for operation
Calibration: In light intensity
calibration
Preheating: In preheating
Preheat Complete: Preheatingcompleted
Measuring&Heating: In absorbancemeasurement
Cooling: In cooling(5) Preheating start button
Valid only in the “Ready” state.
(7) Process stop button
Valid only in the “Calibration,”“Preheating,” “Preheat Complete,”and “Measuring&Heating” states.
(8) Parameter file name input field(10) Parameter file save button(9) Parameter file read button
Figure 4-7: Thermostatic absorbancemeasurement process screen(11) Thermostatic absorbancemeasurement result display
button(7) (1) Enter “Delay Time*.” If not specified or specified as “0,” zero adjustment andabsorbance measurement will start just after the “Start” button is tapped.
* Delay time: Time taken before the start of zero adjustment and absorbance measurement(8) Set the intended values for the HeatLid temperature, Well temperature, and heatingtime (2).
When all parameters are specified, the status indication changes to “Ready”(Figure 4-8). By specifying the temperature and time in (3), you can also define
two-step temperature change (for example, for enzyme deactivation).
If the heating time is set to “0” or parameters are not defined at all in (3), two-stepprocess is not performed.

30

MyAbscope® Instruction Manual(Effective range of temperature and time for thermostatic absorbance measurement unit)
They can be specified up to one decimal place in the following range:
Step 1 Heat Lid temperature: 35.0 to 80.0°C, Well temperature: 35.0 to 70.0°C,
Time (heating time): 1 to 65 minutes** Including Delay Time. For example, if Delay Time is set to 300 seconds, the effective range ofTime (heating time) is 1 to 60 minutes.

Step2

Heat Lid temperature: 35.0 to 80.0°C, Well temperature: 35.0 to 80.0°C,
Time (heating time): 0 to 20 minutes* If a value out of the above ranges is entered, an error message “Setting value is outof range.” is displayed.

(How to save parameters)
To save the parameters, tap the parameter file save button (10) in Figure 4-7, enter the file name on the
save screen shown in Figure 4-8, then tap “Save.” If you tap “Cancel,” the file is not saved.
If a file with the same name exists, tap “Yes” on the confirmation screen shown in Figure 4-9 to overwrite it,or tap “No” to return to the parameter save screen.

Figure 4-8: Parameter save screen

Figure 4-9: Overwrite confirmation screen

To read the saved parameters, select the parameter file name in (8) in Figure 4-7, then tap the parameter
file read button in (9).

31

MyAbscope® Instruction Manual[B: Light intensity calibration]<Main unit>
Thermostatic absorbancemeasurement unit
(for 0.2 mL tubes)

Figure 4-10: Thermostatic absorbance measurement unit (Left)

Applications: Isothermal nucleic acid amplification reaction, enzyme reaction
measurement etc.

<Setting samples>(9) Slowly raise the heat bonnet.

Figure 4-11: Setting a 0.2 mL tube to the thermostatic absorbance measurement unit

(10) Set 8-strip 0.2 mL tubes containing at least 25 L of distilled water each to thethermostatic absorbance measurement unit (Figure 4-11). At that time, make surethat the caps of the 0.2 mL tubes are firmly closed.
If distilled water is less than 25 L per tube, you cannot calibrate the light intensity
accurately.

(11) Slowly lower the heat bonnet and close it completely.
Handle the heat bonnet with care. It may be damaged by rough handling.

32

MyAbscope® Instruction Manual(12) Tap “Calibration,” the light intensity calibration start button, (4) in Figure 4-7 to start

light intensity calibration, displaying “Calibration” (Figure 4-12). (During calibration,
the pilot lamp beside the Power button on the main unit blinks in blue, and thegreen circle  on the lower right of the tablet screen changes to a red circle .
Light intensity calibration will take about three minutes.)
When the light intensity is calibrated for all channels, the indication changes from
“Calibration” to “Ready.”

Figure 4-12: Light intensity calibration screen(How to quit light intensity calibration halfway)
To quit light intensity calibration halfway, tap the process stop button (7) in Figure 4-7. You will be
asked “Are you sure you want to stop?” as shown in Figure 4-13. Tap “Yes” to stop, or “No” to continue
the light intensity calibration treatment.

Figure 4-13: Process stop confirmation screen

33

MyAbscope® Instruction Manual(13) Open the heat bonnet and pull out the 8-strip 0.2 mL tubes containing distilledwater from the thermostatic absorbance measurement unit.

[C: Preheating](14) Tap “Preheat,” the preheating start button, (5) in Figure 4-7 to start preheating,displaying “Preheating” (Figure 4-14). The Well temperature is raised to the preset
temperature before the reaction. When heating starts, the pilot lamp beside thePower button on the main unit blinks in blue, and the green circle  on the lower
right of the tablet screen changes to a red circle . During preheating, the HeatLid
temperature and Well temperature are updated every 10 seconds in the “Running”
fields. When the HeatLid temperature and Well temperature have respectively
reached the preset temperature, the indication changes to “Preheat Complete,”and the time elapsed is displayed (Figure 4-15).
If absorbance measurement is not started within 15 minutes after the preset preheattemperature is reached, cooling automatically starts.

Figure 4-15: Display of measured
temperature and time elapsed
and indication of Preheat
Complete state
Figure 4-14: Indication of Preheating state

34

MyAbscope® Instruction Manual

If the thermostatic absorbance measurement start button (6) in Figure 4-7 is not tapped within 15
minutes after the preset preheat temperature is reached, you will be informed that the data is saved
as shown in Figure 4-16. Then tap “OK.” Three seconds after 15 minutes have elapsed, the cooling fan
starts to operate, and the status indication changes to “Cooling” as shown in Figure 4-17. When both the
HeatLid temperature and Well temperature have fallen below 35°C, cooling is finished, and the statusindication changes to “Ready” as shown in Figure 4-18.

Figure 4-16: Indication of Saved state

Figure 4-18: Indication of Ready state

Figure 4-17: Indication of Cooling state

35

MyAbscope® Instruction Manual(How to quit preheating halfway)
To quit preheating halfway, tap the process stop button (7) in Figure 4-7. You will be asked “Are you sure
you want to stop?” as shown in Figure 4-19. Tap “Yes” to stop, or “No” to continue preheating.

Figure 4-19: Preheating stop confirmation screen
If you tap “Yes,” you will be informed that the data is saved as shown in Figure 4-20. Then tap “OK.” Theprocess data before the stop is saved in the CSV format (with the file name entered in the input field (1) on
the thermostatic absorbance measurement process setting screen, which is suffixed with“_yymmddhhmmss.csv” (see “5. Data Analysis,” (CSV file)). Three seconds after you tap “Yes,” the cooling
fan starts to operate and the status indication changes to “Cooling” as shown in Figure 4-21. When both the
HeatLid temperature and Well temperature have fallen below 35°C, the status indication changes to“Ready” as shown in Figure 4-22.

Figure 4-22: Indication of
Ready state

Figure 4-20: Indication of Saved state
Figure 4-21: Indication of Cooling state

36

MyAbscope® Instruction Manual[D: Thermostatic absorbance measurement](15) Set the 0.2 mL tubes containing the sample to be subjected to thermostatic

absorbance measurement to the thermostatic absorbance measurement unit.
If the sample volume is less than 25 L, the absorbance cannot be measured accurately.

(16) If you tap the reaction process start button (6) in Figure 4-7 within 15 minutes after

the preset preheat temperature is reached, absorbance measurement starts after
setting the initial value (zero adjustment), and the thermostatic absorbancemeasurement result screen is displayed (Figure 4-23). The status indication
changes to “Measuring&Heating” on the setting screen (Figure 4-24 on the nextpage). During absorbance measurement, Abs values (up to four decimal places)
are updated in real time every 20 seconds, and at the same time, themeasurement results are plotted on graphs in real time (Figure 4-25 on the nextpage). By tapping the relevant judgment result field, you can hide the(unnecessary) graphs of unmeasured Wells (Figure 4-26 on the next page).
After the first-step reaction is completed, the second-step process is automatically
performed at the preset temperature and time.
HeatLid temperature, Welltemperature: Updated every 10
seconds (Not displayed when notconnected).
Time elapsed: Starts to be counted
when both the HeatLid
temperature and Well temperature
have respectively reached thepreset temperature ±1°C (for bothreaction and two-step processes).

Abs value: Updated every
20 seconds during lightmeasurement (displayed up
to four decimal places).

Judgment result field+: If Abs value > Threshold (High)−: If Abs value < Threshold (Low)±: If Threshold (Low) ≤ Abs value≤ Threshold (High)Plot (graph) display ofabsorbanceFigure 4-23: Thermostatic absorbance measurement result screen

37

MyAbscope® Instruction Manual

Figure 4-24: Indication of Measuring&Heating state

Figure 4-25: Graph display during
absorbance measurement

Figure 4-26: Switching show/hide formeasured data

38

MyAbscope® Instruction Manual(How to quit thermostatic absorbance measurement halfway)
To quit thermostatic absorbance measurement halfway, tap the process stop button (7) in Figure 4-7. You
will be asked “Are you sure you want to stop?” as shown in Figure 4-27. Tap “Yes” to stop, or “No” tocontinue the thermostatic absorbance measurement.

Figure 4-28: Process stop confirmation screen
If you tap “Yes,” you will be informed that the data is saved as shown in Figure 4-28. Then tap “OK.” Theprocess data before the stop is saved in the CSV format (with the file name entered in the input field (1) on
the thermostatic absorbance measurement process setting screen, which is suffixed with“_yymmddhhmmss.csv” (see “5. Data Analysis,” (CSV file)). Three seconds after you tap “Yes,” the cooling
fan starts to operate and the status indication changes to “Cooling” as shown in Figure 4-29. When both the
HeatLid temperature and Well temperature have fallen below 35°C, the status indication changes to“Ready” as shown in Figure 4-30.

Figure 4-30: Indication of
Ready state

Figure 4-28: Indication of Saved state
Figure 4-29: Indication of Cooling state

39

MyAbscope® Instruction Manual[E: Finishing measurement and taking out sample](17) After the preset second-step process time has elapsed, you will be informed thatthe data is saved as shown in Figure 4-31. Then tap “OK.” The process data is
saved in the CSV format (with the file name entered in the input field (1) on thethermostatic absorbance measurement process setting screen, which is suffixedwith “_yymmddhhmmss.csv” (see “5. Data Analysis,” (CSV file)). Three seconds
after the preset second-step process time has elapsed, the cooling fan starts tooperate, and the status indication changes to “Cooling” as shown in Figure 4-32.
When both the HeatLid temperature and Well temperature have fallen below 35°C
(within about 10 minutes or less), cooling is finished, and the status indication
changes to “Ready” as shown in Figure 4-33.
Hot air may come out from the back of the heat bonnet. Be careful of burns.

Figure 4-33: Indication of
Ready state

Figure 4-31: Indication of Saved state
Figure 4-32: Indication of Cooling state

40

MyAbscope® Instruction Manual<Taking out sample>(18) Slowly raise the heat bonnet.
Handle the heat bonnet with care. It may be damaged by rough handling.

(19) Pull out the 0.2 mL tubes containing samples (Figure 4-34).
Tubes may be hot. Be careful of burns.

Figure 4-34: Taking tubes out of the thermostatic absorbance measurement unit

* To continue thermostatic absorbance measurement, restart it from entering the filename as described in [A: Setting reaction conditions] on page 27.

<Shutting down main unit>(20) Press the Power button on the main unit. The pilot lamp beside the Power buttonon the main unit will turn OFF.

41

MyAbscope® Instruction Manual

5. Data Analysis<Confirming data on application>(1) Tap “Data” on the top screen (Figure 5-1). The detailed data confirmation screen isdisplayed as shown in Figure 5-2.

Figure 5-1: Top screen(1) Stored data nameinput field(2) Judgment result field+: If Abs value > Threshold (High)−: If Abs value < Threshold (Low)±: If Threshold (Low) ≤ Abs value≤ Threshold (High)

Figure 5-2: Detailed data confirmation screen

42

MyAbscope® Instruction Manual(2) Tapping the stored data name input field (1) in Figure 5-2 displays the file nameselection screen shown in Figure 5-3. Select the file name you want to check andtap “OK,” then the data and graphs are displayed as shown in Figure 5-4.

Figure 5-3: File name selection screen

Figure 5-4: Detailed data screen(3) By tapping the judgment result field (2) in Figure 5-2, you can hide the graph of therelevant channel as shown in Figure 5-5. To redisplay the hidden graph, tap therelevant judgment result field again.

Figure 5-5: Switching show/hide for the graph of each channel

43

MyAbscope® Instruction Manual<Confirming data on PC (Windows 7 or 10)>(1) Connect the tablet to a PC using the accessory USB cable ferrite core is attached of
the tablet.The CSV file is saved in the following internal storage folder of the tablet.
Computer¥Nexus 9¥internal storage¥Android¥data¥com.kaneka.myabscope¥files

Select “Open the device to show files” on the automatic reproduction screen shownin Figure 5-6.

Figure 5-6: Automatic reproduction screen(2) Open “Android” in the internal storage.

Figure 5-7: Internal storage folder

44

MyAbscope® Instruction Manual(3) Open “data” in “Android.”

Figure 5-8: Android folder(4) Open “com.kaneka.myabscope” in “data.”

Figure 5-9: data folder

45

MyAbscope® Instruction Manual(5) Open “files” in “com.kaneka.myabscope.”

Figure 5-10: com.kaneka.myabscope folder(6) Open the intended CSV file in “files.”Figure 5-11: files folder

46

MyAbscope® Instruction Manual(7) The CSV file is opened as follows: The absorbance data is shown in row 22 andafter in columns A through I (enclosed with a red frame in Figure 5-12). The preset
data is shown in the blue frame in Figure 5-12.

<Enlarged view>
Figure 5-12: Absorbance data recorded in a CSV file47

MyAbscope® Instruction Manual

Cleaning and Storage
Do not wipe this product with organic solvent. Disconnect the product from the power
supply before cleaning.

Cleaning⚫

Main unit
If the main unit gets dirty, gently wipe off the dirt with a clean soft cloth dampened
with a neutral detergent diluted with water, then wipe off water with a clean dry soft
cloth.

⚫

Thermostatic absorbance measurement unit
If the sample ports get dirty, gently wipe off the dirt with a clean soft cloth or cottonswabs.
If you use a hard cloth to wipe off dirt, the optical unit may be scratched, resulting inincorrect measurement.

48

MyAbscope® Instruction Manual⚫

Fan filter
If the fan filter is clogged, open the filter outlet on the bottom of the main unit, takeout the fan bracket, and remove the dust. After cleaning the filter, insert the fan
blanket in the direction shown in the lower right figure, then close the lid.

⚫

Long-term storage
If you do not use this product for a long time, firmly close the heat bonnets of thehigh-temperature treatment unit and thermostatic absorbance measurement unit,
and store the product avoiding direct sunlight, dust, and high temperature andhumidity.

49

MyAbscope® Instruction Manual

Trouble shooting
Q1. It is not possible to begin treatment as the instrument stops before reaching the set
temperature.
A1. If it does not reach the set temperature within twenty minutes after commencing
temperature control when conducting high-temperature treatment or when measuring
constant-temperature absorbance, then the heating process will automatically stop.
Re-start treatment after re-starting both the instrument and the App.
Q2. The App continues to display “cooling” even after the cooling fan has stopped, and it
is not possible to start the next treatment.
A2. If it continues to display “cooling” even after the cooling fan has stopped, thenre-start the App.
Q3. The App does not operate properly.
A3. Make sure that the PC (Windows) you are using is connected to the instrument, andthen re-start the App.
Q4. My PC is not connecting properly. (The COM Port number of the instrument is not
displayed even though the COM Port Search Button has been depressed.)A4-1. Try pressing it several times.
A4-2. Make sure that the instrument is ON.
A4-3. Make sure the Bluetooth on the instrument is OFF. It cannot connect to the PC
when the Bluetooth is ON.
A4-4. Re-start both the instrument and the App.
Q5. It is not possible to properly measure absorbance.
A5-1. Check the absorption wavelength range of the sensor you are using.
A5-2. Make sure that you are using at least 25 μl of sample solution and that the sample
solution is free of bubbles and deposits.
If the tube has scratches, please use a new one.
A5-3. Samples have multiple absorption peaks, and measurements may not be possible
if these peaks fluctuate individually.
Q6. Which sensor should I use for constant-temperature absorbance measurements?
A6. The absorption wavelength ranges for each sensor are listed on page 28（on page
10 of the simple manual）. Select the sensor for which the wavelengths that
increase/decrease by reaction is included in the absorption wavelength range.

50

MyAbscope® Instruction Manual

Q7. Is it possible to conduct measurements using the R, G and B sensors
simultaneously?
A7. It is not possible to conduct measurements simultaneously. Select one sensor foreach measurement.
Q8. Heating cuts off during the pre-heating process. Why is this?
A8. If the instrument is left alone for 15 minutes after the pre-heating process has been
completed, it shuts off for safety reasons.
Q9. When conducting constant-temperature absorbance measurements, is theabsorbance measurement data in Step 2 saved?
A9. Absorbance measurement data is not saved in Step 2. Only Step 1 is saved.
Q10. Is light intensity calibration necessary for each measurement ?
A10. It is absolutely necessary after launching the App. It is also necessary to conduct
light intensity calibration when changing the selected sensor. When conducting
constant-temperature absorbance measurements in succession with the same sensor, it
is not necessary to conduct light intensity calibration after the second time.
Q11. Is it possible to use batteries?
A11. The instrument is confirmed to operate using a mobile battery 23000mAh“700-BTL017BK” (manufactured by Sanwa Supply in Japan). The instrument may beunusable with some battery types.

51

MyAbscope® Instruction Manual

After-sales Service
If something is wrong✓

Restart the application.

✓

Turn OFF and then ON the main unit and tablet.

✓See “Troubleshooting” above.

If the problem is not fixed even after that, or how to deal with the problem that is notdescribed in “Troubleshooting” above, please contact your dealer.

When you contact us, please inform us of the following.


Model



Serial numberDate of purchaseDealer

Failure situationUse history

Contact
Medical Devices Solutions Vehicle, Kaneka Corporation
Email: info_dquick@kaneka.co.jp
Open: 9:00 AM to 5:00 PM (excluding Saturdays, Sundays, and national holidays)

52

MyAbscope® Instruction Manual

Specifications
Item
External dimensions
WeightPower supply
Measurement
Temperature
setting range

Temperature
control accuracy

Heating rate

Temperatureholding time
setting range
Cooling method
Number ofsamples

Measurement
interval
Absorbancemeasurement
Sensor unit
Light source
Detector
Minimum sample volume
External interface
Power
Voltage
Current
DC plugCompatible
Tablet (wireless)devicePC (wired)
Language
Data output
Storage format
Destination folder

Specifications
H92 x W312 x D190 (mm)2 kg (excluding AC/DC adapter)
AC/DC adapter 19 V DC
<Sample chamber>
Thermostatic absorbance measurement unit (with 8
wells): 35 to 70°C
High-temperature treatment unit (with 4 wells): 35 to
100°C<Heat bonnet>
Thermostatic absorbance measurement unit: 35 to
80°C
High-temperature treatment unit: 35 to 120°C
Thermostatic absorbance measurement unit (with 8
wells): 63 ± 1°C
High-temperature treatment unit (with 4 wells): 95 ±4°C
Thermostatic absorbance measurement unit (with 8
wells): Reaches 63°C within 5 minutes
High-temperature treatment unit (with 4 wells):
Reaches 95°C within 10 minutes
Thermostatic absorbance measurement unit: 1 to 65
minutes
High-temperature treatment unit: 1 to 10 minutes
Cooling by internal fan
Thermostatic absorbance measurement unit: 8 tubes(0.2 mL tubes)
High-temperature treatment unit: 4 tubes (1.5 mL
tubes)
20 seconds
Up to four decimal places
White LED
RGB color sensors
25 L
Bluetooth Low Energy (Bluetooth smart) or USB
19 V DC
2A
φ5.5/2.1, L = 9, Plastic part: φ11.5 or less
Nexus 9, ZenPad 3 8.0 (Z581KL), MediaPad M3 Lite 10
Windows 7, Windows 10
EnglishCSV file
com.kaneka.myabscope

53

MyAbscope® Instruction Manual

Quality Assurance
1. The warranty period of this product is one year from the date of purchase. If a failure
occurs during the warranty period, we will exchange the product for free provided
that a document that shows the date of purchase is submitted.
2. Warranty exclusions
Failure of this product caused by any of the following reasons is not covered bywarranty.
⚫ Failure due to conditions, environments, handling, or usage other than thosedescribed in this manual, catalog, or other technical data
⚫ Repair or alteration by anyone other than Kaneka’s customer service personnel
or those specified by Kaneka⚫ Failure due to any factor other than that attributable to this product, such as thedesign of the customer's device or software⚫ Failure due to external factors for which we are not responsible, such as natural
disasters⚫ Failure due to reasons that could not be predicted by the level of technology at
the time of shipment⚫ Failure due to noncompliance with laws, regulations, or safety standards outside
Japan (such as CE, VL, and CSA) except those separately agreed with thecustomer
3. Disclaimer⚫ Regardless of the warranty period, we will not be liable for any loss of opportunity,
loss of business profits, secondary disasters, damage to machines other than
this product, and employment injuries caused by the failure of this product for
which we are not responsible.
⚫ We will not take responsibility for any loss, such as data loss and malfunction,
caused by radio wave interference with devices other than this product for which
we are not responsible.
4. Others⚫ Service parts for this product are stored until five years have elapsed fromdiscontinuation.

Date of purchase

DealerPhone:()
1901

54