2014 Annual Meeting of The Institute of Electrostatics JapanHiroshima International University
September 7-8, 2014

9pC-1
September 9th (Tue.), <14:00-15:00>
Room 1

2-D Concentration Distribution of ROS Supplied on Liquid
Target by Non-thermal Plasma Jet○Masaki HAMADA, Yasutaka WAKABAYASI, Wataru ETO, Yasuhumi ABE,Keisuke KIHARA, Miho SAKAI, and Toshiyuki KAWASAKI
Faculty of Engineering, Nippon Bunri University

Abstract:
The distribution of reactive oxygen species (ROS) generated by aplasma jet has been investigated using the chemical reagent prepared in ourlaboratory. This chemical reagent provides the important and special information
which can’t be obtained by other methods. In this paper, the influence of theirradiation distance, supplied gas and water on the 2-D ROS distribution wasstudied using the chemical reagent. Helium (He) gas with or without oxygen (O2)were supplied into the generator. The irradiation distance was changed from 5mm to 50 mm. The experiment with the water layer on the reagent was also
conducted in order to study the production and transport of ROS through water.
The relative concentration distribution was also obtained by an absorbancemeasurement. The ROS distribution significantly depended on the irradiation
distance in He plasma jet. The high efficiency and local supply of ROS to thetarget can be controlled by the irradiation distance. In the case of O2 (0.5%)/He
mixture gas supply, it is considered that the ROS distribution significantly
influenced by the synergistic effect of long-lived species generated in the plasmaphase. The interesting 2-D distribution patterns were visually obtained in thewater using the reagent underwater.

2014 Annual Meeting of The Institute of Electrostatics Japan
Hiroshima International University
September 7-8, 2014

9pC-2
September 9th (Tue.), <14:00-15:00>
Room 1

Influence on Plants by Irradiation of Atmospheric-pressure
LF Plasma Jet○Kei ETO, Daichi OTORI, Shuichi AKAMINE, Ryuta ICHIKI, Seiji KANAZAWA
Department of Electrical and Electronic Engineering, Oita University

Abstract:Inthisstudy,
Arabidopsis
thaliana
wasirradiatedbyatmospheric-pressure low frequency (LF) plasma jet. Here, helium gas was used
as an operating gas. We evaluated the influence of the atmospheric-pressureplasma jet on growth of the plants by measuring the leaf area of Arabidopsis
thaliana and comparing with a control group. We also measured chlorophyll
fluorescence of leaves. As a result, the effect of atmospheric-pressure plasma
jet was different with state and form of plants. The growth stimulation wasobserved for the seeds irradiated by atmospheric pressure-plasma jet aftervernalization. After germination the apoptosis occurred for the leaves irradiatedby the plasma jet. This inactivation was related to the intensity decrease ofchlorophyll fluorescence from the leaves.

2014 Annual Meeting of The Institute of Electrostatics Japan
Hiroshima International University
September 7-8, 2014

9pC-3
September 9th (Tue.), <14:00-15:00>
Room 1Analysis of Biomolecular Damage and Cellular Responses
Induced by Atmospheric Pressure Plasma Exposure○Hirofumi KURITA*, Kaori SANO*, Mika SHIMIZU*, Tomoko NAKAJIMA*, Kazue
MIZUNO**, Ryo ONO**, Hachiro YASUDA*, Kazunori TAKASHIMA*, and Akira
MIZUNO**Toyohashi University of Technology, **The University of Tokyo

Abstract:
Recently, non-thermal atmospheric pressure plasma has been studied inbiological and medical applications. Among them, reactive oxygen andnitrogen species (RONS) in aqueous solution injected by the plasma exposure
play an important role. Therefore, we have been trying to use large DNA
molecules as a biomarker to estimate intensity of RONS in the aqueous media.
Here, we report the measurement of OH radical by electron spin resonance(ESR) spectroscopy with spin trapping technique. The correlation between thesignal intensity of OH radical measured by ESR and the number of strand
breaks obtained by single-molecule DNA observation was examined.
Furthermore, cellular responses after plasma exposure in human cell lines werealso studied.

2014 Annual Meeting of The Institute of Electrostatics JapanHiroshima International University
September 7-8, 2014

9pC-4
September 9th (Tue.), <14:00-15:00>
Room 1

DNA Detection using Dynamic Change of Microbeads
Dielectrophoresis with DNA Labeling○Michihiko Nakano, Zhehnao Ding, Hiromichi Kasahara, Junya Suehiro
Kyushu University

Abstract:
In this paper, a new DNA detection method using dielectrophoresis ofdielectric microbeads is described. This method can detect DNA amplified bypolymerase chain reaction (PCR) rapidly and easily. PCR is used to diagnosebacterial/viral infection as the one of the most sensitive and specific methods.
PCR amplifies DNA for the target pathogens. After PCR, the amplified DNA
should be determined by a method such as an agarose gel electrophoresis. Thistakes a few hours and requires complicated processes. It was found thatdielectrophoresis of the microbeads was dramatically altered by DNA
immobilization on them. The DNA-labeled microbeads were trapped on a
microelectrode by positive dielectrophoresis, whereas the pristine ones wererepelled from the microelectrode. The trapped microbeads were measured byelectrically. This method is faster than a standard DNA detection.