Multiphoton Laser Scanning Microscope

FVMPE-RSFLUOVIEW

See More Detail at DepthHigh Speed
Extended
IR Range
Deep Observation

Inverted
Microscope System

1

Multi-color
Laser Light
Stimulation

High-Speed Imaging at Depth
The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing
both detail and dynamics. Innovative features for efficient delivery and detection of photons in scattering media enable highsignal-to-noise ratio acquisition. This translates to bright images with precise details — even from deep within the specimen.
High sensitivity is matched with high-speed imaging to capture rapid in vivo responses. For advanced applications,dual-wavelength excitation extending to 1300 nm is available. Independent control of visible or multiphoton laser lightstimulation and the ability to synchronize with patch clamp data are also possible.
Multiple configurations are available on the FVMPE-RS platform; each imaging system is customizable to meet your uniqueresearch requirements.

Upright
Microscope System

GantryMicroscope System2Designed for Deep Imaging
Maximize Resolution in Deep Imaging
Olympus TruResolution objectives maximize resolution and contrast for 3D imaging deep within thick specimens. The objectives are equippedwith a motorized correction collar that can automatically and dynamically compensate for spherical aberration while maintaining focus
position. Refractive index mismatch between the sample and immersion medium introduces spherical aberration that degrades the focus ofan objective lens. This aberration worsens deeper into the sample, yielding progressively poorer images with depth. TruResolution objectives
solve this challenge with an autocorrection collar. The system can be driven either by an algorithm that finds the best collar setting at eachlayer of a z-stack or by user-defined manual presets to deliver consistently bright and sharp images through out the captured volume.
0

TruResolution Objectives

200

400

600

With
TruResolution

FV30-AC10SV

10X multi-immersion
NA 0.6, W.D. 8mm

FV30-AC25W

25X water-immersion
NA 1.05, W.D. 2mm

Without
TruResolution

XZ images of 0.2μm fluorescent micro spheres in scattering gel (RI=1.36)
at various depth acquired by using FV30-AC25W.

3D reconstructed image of mouse in vivo brain (Thy1-YFP-H mouse, sensory cortex) acquiredby using TruResolution objective with auto adjustment function.
Image data courtesy of: Dr. Hiromu Monai, Dr.Hajime Hirase and Dr. Atsushi Miyawaki,
RIKEN BSI-Olympus Collaboration Center.

Without TruResolution

With TruResolution

Maximum projection of images acquired at around 600um depth.
Image data courtesy of: Dr. Hiromu Monai, Dr.Hajime Hirase and Dr. Atsushi Miyawaki,
RIKEN BSI-Olympus Collaboration Center.

Maximize Signal with Deep Focus Mode
Deep focus mode adjusts the diameter of the laser beam based on
the laser scattering conditions. For in vivo specimens with heavy
laser scattering, the beam is narrowed so that more excitation
photons reach deeper within your sample, helping produce bright,
high-resolution images.
Laser Beam Diameter(Large)Laser Beam Diameter(Large)ScatteredLight

Both maximum intensity projection from 23 slices,
Deep Focus Mode improves image brightness

Laser Beam Diameter(Small)Scattered
Light

Normal image
Specimen with
Low Scattering

3

Specimen with
Heavy Scattering

Specimen with
Heavy Scattering

Deep Focus Mode image

Image data courtesy of: Urs Ziegler and Jose Maria Mateos,
Center for Microscospy and Image Analysis, University Zurich.
Mouse line L15 kindly provided by Pico Caroni, FMI, Basel

800 μmObserve In Vivo and Cleared Tissue Specimens Up to 8 mm Deep
Olympus dedicated multiphoton objectives are optimized for deep
imaging under in vivo and cleared tissue conditions. A diverse
lineup provides you the opportunity to select an objective according
to your research requirements. Different optical designs emphasize
high numerical aperture, long working distance, wide field of view,and compatibility with a range of immersion media and tissue
clearing agents.

XLPLN10XSVMP
W.D. 8mm

XLPLN25XWMP2
W.D. 2mm

Wide Field of View
Wide fields of view
enable these objectives
to efficiently collectscattered fluorescencephotons and generatebrighter images fromdeep within turbid
specimens.

Dedicated Multi Photon Objectives

NA

W.D.(mm)

Immersion Index

XLPLN10XSVMP

0.6

8.0

1.33–1.52

XLPLN25XWMP2

1.05

2.0

1.33

XLPLN25XSVMP2

1.00

4.0

1.33–1.40

XLSLPLN25XSVMP2

0.95

8.0

1.33–1.40

XLSLPLN25XGMP

1.00

8.0

1.41–1.52

XLPLN25XSVMP2
W.D. 4mm

XLSLPLN25XSVMP2
W.D. 8mm

XLSLPLN25XGMP
W.D. 8mm

Silicone Oil Immersion for Live Cell Imaging
The UPLSAPO30XSIR objective applies the efficient IR transmission
coating of MPE dedicated objectives to a high numerical aperturesilicone oil immersion objective. This combination makes theobjective well-suited to multiphoton imaging of live cell specimens.
The closer refractive index matching between silicone oil and live
cells minimizes image distortion in the Z-direction and improves
image brightness and resolution.
During time-lapse imaging, the refractive index of silicone oil remains
constant, and the oil does not dry out, minimizing the amount of time
researchers need to spend tending the experiment.

Silicone Immersion Objective
UPLSAPO30XSIR
Magnification: 30x
NA: 1.05 (silicone immersion oil)
W.D.: 0.8 mm
Cover glass thickness: 0.13–0.19 mm
Operation temperature: 23ºC–37ºC

Refractive Index is Important with
Deep Tissue Observation
Water immersion objective

Silicone immersion objective
Specimenne≈1.38

Water
ne≈1.33

Cover glassne≈1.52

When working with a water
immersion objective, the difference
between the refractive index of thesample and water results inspherical aberration in deep tissue,
causing resolution to deteriorate
and fluorescence to become dim.

Visible to IR Transmission with Olympus 1600 Coating

When working with a silicone
immersion objective, the difference
between the refractive index of thesample and silicone oil is minimal.
So it achieves brighter fluorescence
images with higher resolution fordeep tissue observation.

Transmittance Curve of Objective
Transmittance (%)

Olympus multiphoton objectives and scanner optics have an optical coating that offers
excellent transmission from 400 nm to 1600 nm. Efficient infrared transmission translates
to more available power for fluorescence excitation at depth while strong support at shortwavelengths maintains efficient collection of fluorescence and harmonic emissions.

Silicone oil
ne≈1.40

100
90
80
70
60
50
40
30
20
10
0
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600
Wavelength (nm)Conventional XLPLN25XWMPXLPLN25XWMP2 with 1600 Coating4Capture Fast, Dynamic Cellular Processes
High-Speed, 438 Frames-per-Second Scanning
A fast resonant scanner and conventional galvanometer scanner
provide high-speed and high-resolution imaging in a single
system. Avoid motion artifacts when imaging dynamic samples
with capture rates of 30 fps at 512 × 512 pixels at the full field ofview (FN 18) or up to 438 fps at 512 × 32 pixels. These speeds
enable applications such as tracking of fast moving cells in blood
flow, and observing rapid membrane potential dynamics acrossneurons and other cells.

Galvanometerscanner

Resonant scannerHigh speed imaging

0ms
2ms
4ms
6ms
41ms
282ms
520ms
543ms

Efficient Laser Transmission
100
90
80

Reflectance [%]Olympus silver-coated scanner mirrors help deliver more laser power to your sample to better excite fluorescence, and yield brighter
images. The silver-coated mirrors achieve very high reflectanceacross a broad wavelength range, from visible to near infrared. The
total reflectance for the XY scanner is particularly improved in thenear infrared range compared to conventional aluminum-coatedmirrors. The increased reflectance helps maximize available laser
power and deliver the power needed for deep in vivo experiments.

70
60
50
40
30

Silver coating

20

Aluminum coating

10
0
400

500

600

700

800

900

1000

Wavelength [nm]

5

1100

1200

1300

A Cooled, High-Sensitivity GaAsP Detector Acquires High S/N Images
High signal-to-noise ratio imaging can be acquired even from faint fluorescence through the use of high sensitivity gallium arsenide
phosphide (GaAsP) photomultiplier tube (PMT) detectors. — GaAsP PMTs deliver greater quantum efficiency than standard multialkali PMTs.
Fan-less Peltier cooling further improves the signal-to-noise ratio. You can also leverage the advantages of both detector types by combining
them in a single system.

Image captured with multialkali detector

Image captured with GaAsP detector

Arc-dVenus transgenic mouse (8-week-old), coronal brain block, hippocampal
dentate gyrus
Projection image of 300 — 400 μm depth (5 μm steps)
Image data courtesy of:
Dr. Norio Takata, Dr. Hajime Hirase Laboratory for Neuron-Glia Circuitry, RIKEN BSI
Dr. Shun Yamaguchi Gifu University Graduate School of Medicine

Greater Fluorescence Capture
The non-descanned detection path has been designed for greaterlight efficiency—it is positioned close to the specimen and featureslarge area optics to better capture scattered fluorescence photons.

Microsecond Timing for Electrophysiology and Optogenetics
A hardware sequencer provides microsecond precision timing forstimulation and triggering events. Stimulation can be spatially andtemporally synchronized to the imaging scan, facilitating the captureof fast response dynamics at precise locations. In the context ofelectrophysiology and optogenetics, this could mean the difference
between distinguishing a synchronous versus an asynchronous
stimulus response. For acquisitions lasting two weeks or longer
or experiments with complex procedures that require switching
between imaging tasks, the sequence manager software module
maintains millisecond precision, providing high-quality data indemanding in vivo and in vitro experiments.

XY control

XYZ control

XYZ + stimulation control

6

Spend Less Time Tuning the Laser:
Flexible Dual-Line Multiphoton Imaging
Multiwavelength Excitation for Multiphoton Imaging
The FVMPE-RS imaging platform supports a dual wavelength infrared pulsed laser or two independent infrared lasers for multichannel, multiphoton
excitation imaging. You can optimally excite different fluorophores without having to repeatedly tune the laser. Simultaneous excitation withindependent power control of each laser line enables users to capture balanced images of different fluorophores. Separate excitation wavelengths
for individual fluorophores can also reduce background tissue autofluorescence by shifting excitation away from the 800 nm range.

Efficient Longer Wavelength Excitation
The InSight X3 pulsed IR laser by Spectra-Physics supports
multiphoton imaging with tunable excitation from 680–1300 nm. The
dual-line version adds a second fixed line at 1045 nm. With improved
laser power beyond 1000 nm, the InSight X3 laser provides accessto new multiphoton imaging capabilities. Utilize the growing libraryof red-shifted dyes and fluorescence proteins for deeper imaging or
broader multichannel coverage. Perform third harmonic generation
imaging in biological specimens without UV damage.
Crosstalk occurred after simultaneous
excitation of GFP and DS-RED with a single
wavelength IR pulsed laser(950 nm).

Two fluorescence proteins are clearly
separated after individual excitation of GFP and
DS-RED with a dual wavelength IR pulsed laser(950 nm/1040 nm).

Image data courtesy of:
Director Naoki Mochizuki
Department of Cell Biology, National Cerebral and Cardiovascular Center

Third harmonic generation imaging of porcine adipose tissue
Unlabeled porcine adipose tissue was irradiated with femtosecond laser at 1250 nm, second harmonicis detected from collagen fibers at 625 nm and third harmonic from lipid interfaces at 416 nm.

User-Friendly, Accurate Imaging with Auto Laser Alignment
Quadralign 4 axis laser alignment simplifies system upkeep by maintaining the precise alignment of the excitation beam into the scanner unit,
even in the face of laser drift due to wavelength tuning, temperature fluctuation, and other sources of cavity shift. The beam position andangle are automatically adjusted to deliver higher laser power andconsistent pixel registration. If your system has two excitation laserlines, this feature offers an additional benefit. Auto laser alignment
maintains co-alignment between the beams, helping eliminate coregistration errors between channels. The laser alignment can also be
manually fine-tuned using the software interface.
Laser beam 1

Pixel shift

7

Laser beam 2

Correction of pixel shiftOptional Features for Advanced Applications
Simultaneous Multiphoton Stimulation and Imaging with the SIM Scanner
The SIM scanner, an independent galvanometer scanner, andvisible laser modules can be added for precise microsecond
photostimulation and photobleaching experiments. On systems
with two IR imaging lines, the SIM scanner enables simultaneous
multiphoton stimulation and imaging.

Synchronize Electrophysiological Data and Laser Light Stimulation with the Analog Unit
Analog inputs and TTL I/O are available to support electrophysiology
experiments. The analog input unit records external voltage signals
as images that are treated the same way as normal image data.
Light-stimulated electrical signals measured with patch clamps
can be synchronized with image capture and displayed as a
pseudocolor intensity overlay.

35˚
NA 1.05

W.D. 2mm

Create 3D Stimulation Reaction Maps
To achieve highly targeted laser light stimulation, the observation
field is divided into a grid, and the laser illuminates each area ina pseudorandom sequence that avoids sequential stimulationof adjacent areas. A stimulation reaction map is drawn basedon patch clamp recording or imaging intensity. Integration of an
optional piezo nosepiece extends the reaction map to 3D, withstimulation delivered at depths different from the imaging plane.
A Typical Combination of Laser Light Stimulation and an Analog Unit
Laser Light
Stimulation

Fluorescence image

0
5
10
20

Overlay of morphology
image and reaction map

30
40
(pA)

Going tothe Analog
Unit

Electro-physiology reaction
map recorded by analog unit

*Data acquired with equivalent function in FV1000

10pA
1mA

Image data courtesy of:Haruo Kasai
Center for Disease Biology and Integrative Medicine, Faculty of Medicine,
The University of Tokyo

8

Intuitive Software Optimized for Multiphoton Observation

1. Layout

2. Acquisition Conditions

3. Acquisition

4. Viewer

5. Analysis•Select the layout
based on yourexperiment•Reload the settings fromprevious experiments•Acquire multidimensional images•Acquire multiarea images•Acquire images using a complex
protocol•Review images during
or after the acquisition
using various display
methods•Various image analysis tools
are available•Perform analysis via a oneclick macro

Customizable Layout
Customize which controls you see in the software interface and where they are located. Save and reload your favorite layouts.

Save and Recall Settings
Save the acquisition parameters that you use during an experiment. The parameters can be easily recalled for repeatable imaging conditions.

Precisely Control Your Experiments
The sequence manager makes it easy to coordinate experiments. Complex protocols, such as changing the frame rate during time-lapse
imaging or repeating photostimulation events at different positions during image acquisition, can be organized and accurately carried out with
precise timing. Protocols can also be saved and later reloaded for consistent execution of experiments.

9

Combine Wide Field of View with High Resolution
See your entire specimen at high resolution and in context with thetiling function. This software feature scans multiple adjacent images
and stitches them together. With a motorized stage, images canbe stitched together in a very wide field of view, while the mapping
feature makes it easy to locate the position of specific cells in thelarger image.

Using the map function with motorized stage,finding target field of view is easy
Image data courtesy of:
Urs Ziegler and Jose Maria Mateos
Center for Microscospy and Image Analysis, University Zurich
Mouse line L15 kindly provided by Pico Caroni, FMI, Basel

Expanded Analysis Functions
The FVMPE-RS imaging platform’s software is integrated with Olympus cellSens image analysis software, expanding the system’s analyticalcapabilities. Optional features include 3D deconvolution for Z-stack images, area estimation for each particle in an image, an image
processing filter, and colocalization analysis.

Separate Overlapping Channels with Spectral Deconvolution
Closely overlapping fluorescence spectra can complicate biologicalstudies that look at multiple labels simultaneously. Separation of
overlapping spectral channels is possible via spectral deconvolution
based on either a blind unmixing algorithm or previously saved
multichannel profiles. Cross-talk between the channels can even be
eliminated during image acquisition via live processing.

Mouse kidney tissue stained with
Alexa Fluor 488 WGA and Alexa Fluor 568 Phaloidin
Normal mode

Live unmixing mode

3D Rendering
Large amounts of Z-stack data can be rendered into a 3D display.
Important views can be registered as key frames, making it easy
to create animated views of 3D images that zoom and transition todifferent camera angles.

4 mm 3D stack on blood vessel label with Texas Red in mouse brain
Image data courtesy of:Hiroshi Hama, Rie Ito, Atsushi Miyawaki
Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute10Choose From 3 Frames and 3 Laser Configurations
Designed for Multiphoton Microscopy

Upright Microscope System• Non-descanned detectors are sensitive to ambient light, and theblack microscope frame helps reduce undesired light reflections.
• A large focus stroke accommodates a range of specimens, fromtissue slices to live mice and other small animals.
• The optional transmitted fluorescent light detector expands
the system’s capabilities in multiphoton imaging. The high NA
condenser efficiently collects transmitted fluorescence as well astransmitted second and third harmonic generation signals.
• The motorized fluorescence illuminator has a double deck design
to reduce vibration during mirror unit exchange. It’s easy to change
observation conditions (multiphoton and widefield fluorescence),even during simultaneous patch clamp experiments.

For in vivo Observation that Require Maximum Space

Gantry Microscope System• A removable, manual XYZ stage enables height adjustments.
Changing from thin sample to whole animal imaging is quick andeasy.
• The frame maintains a large working space beneath the objective,
making it easier to position experiment equipment.

DW
H

640(W)×355(H)×520(D)(mm)

For Observing Tissue Cultures, 3D Cultures, and Cell Cultures (Spheroid)

Inverted Microscope System• The frame supports observations of 3D cultures and multicellularclusters that are difficult to image using an upright frame.
• The optical performance of the Olympus IX83 fully automatedmicroscope was optimized to efficiently collect scatteredfluorescence light. Non-descanned detection performance isimproved compared to previous multiphoton systems on inverted
frames.

11

One Laser System
This streamlined system uses a single multiphoton
infrared laser for imaging. Add an optional SIM scannerfor visible laser stimulation.

575

Power
Suppy Unit
& Chiller

900
System Desk

Rack

600

Rack
(Option)

610

550

618

2700

1500

1L1S

1650*
2550

618

Power
Suppy Unit
&610
Chiller

Rack
Rack(Option)Power
Suppy Unit
& Chiller

Rack

900
System Desk

900

600

Rack
(Option)

550

575

610

550

618

Dual

2700 600

System Desk

1500

575

Dual Laser Lines System

600

1500

900

System Desk

Rack

2000
3100

600

550

Tw 2L2S
Dual
20%

1500

1L1S

1650*
2550

2700

Power
Suppy Unit
& Chiller

550

System Desk

Rack
(Option)

610
575

900

618
Rack

1500

575

Power
Suppy Unit
& Chiller

900
System Desk

2700

618

Power
Power
Suppy Unit Suppy Unit Rack Rack
(Option)& Chiller
610 & Chiller

600
2700

Rack

610
575

618
550

1650*
2550

2700

1500

This system supports dual wavelengths for multiphoton,
1L1S
multicolor imaging. Add an optional SIM scanner forvisible laser stimulationand simultaneous multiphoton
1650*
2550
stimulation at 1045
nm.

610

900
System DeskTwin Lasers System

600

Rack

Power
Power
Suppy Unit Suppy Unit 618 Rack
& Chiller& Chiller
Rack(Option)

550

575

610

550

618
1650*
2550

900
600

2700

2700

1500

1500

575

Tw 2L2S
System Desk
Rack
This flexible system employs two multiphoton
Dualinfrared lasers for imaging. In addition to multiphoton,
20% andmulticolor imaging,visible laser stimulation
2000
3100
simultaneous multiphoton
stimulation across tunablewavelengths is also supported in combination with
an optional SIM
scanner.
1650*
Power
Suppy Unit
& Chiller

2550

System Desk

2000
3100

600

Rack

900

1500

575

Power
Power
Suppy Unit Suppy Unit
& Chiller& Chiller

2700

Rack

610

550

618

Tw 2L2S

20%

12

Modular Units Designed for Your Applications
Lasers for Multiphoton Configurations
The InSight X3 Dual-OL laser enables dual wavelength, simultaneous imaging for deep observation. It has a high peak power with short, 120
fs pulse widths, a broad continuously tunable range from 680 nm to 1300 nm, and a fixed wavelength line at 1045 nm. A selection of othermodels are available to match your multiphoton excitation requirements.
INSIGHT X3/X3 DUAL-OL

Manufacturer
Spectra-Physics

MAITAI HPDS/eHPDS-OL

Chameleon Vision I/II-OL

Model

Wavelength covered

Manufacturer

Model

Wavelength covered

MAITAI HPDS-OL

690 nm — 1040 nm

COHERENT

Chameleon Vision I Olympus

690 nm — 1040 nm

MAITAI eHPDS-OL

690 nm — 1040 nm

Chameleon Vision II Olympus

680 nm — 1080 nm

INSIGHT X3-OL

680 nm — 1300 nm

Chameleon Vision S Olympus

690 nm — 1050 nm

INSIGHT X3 DUAL-OL
INSIGHT X3 DUALC-OL

680 nm — 1300 nm
1045 nm (fixed)*Chemeleon Series of lasers are not available from Olympus in some regions.

Visible Beam Combiner for
Laser Light Stimulation

Light Guide Illumination Source(U-HGLGPS)

Transmitted Non-descanned Light
Detector

The laser combiner enables solid-statelaser combinations for laser light stimulation
at wavelengths of 405 nm, 458 nm, and588 nm.

This light source is equipped with a liquid
light guide that minimizes the impactof vibration and lamp heat on both themicroscope and specimens. With a metalhalide bulb, the light source offers anaverage lifetime of 2000 hours.

A high NA condenser and transmitted nondescanned light detector for multiphoton
imaging detect fluorescence and harmonic
emissions scattered in the forward
direction from the sample plane.

Reflected Non-descanned Light Detector
Multialkali PMT 2CH Detector

Cooled GaAsP PMT 2CH Detector

This basic multialkali 2CH PMT provides
robust performance across a wide range of
wavelengths.

This Peltier-cooled 2CH GaAsP PMT
provides higher sensitivity for weak signals
or short pixel dwell times.

Multialkali PMT 4CH Detector

Multialkali PMT 2CH +2CH Cooled GaAsP PMT Detector

Expand your simultaneous detection
capability with a total of 4 multialkali PMTs.

13

Combine the robustness and dynamic
range of multialkali PMTs with the highsensitivity of GaAsP PMTs.

FLUOVIEW FVMPE-RS SPECIFICATIONSOne Laser System

Unit

Qualified IR pulsed
lasers with negative
chirp for multi photon
excitation

Main IR pulsed laser

MAITAI HPDS-OL
MAITAI eHPDS-OL
INSIGHT X3-OL Chameleon Vision I
Olympus
Chameleon Vision II Olympus
Chameleon Vision S Olympus

Additional IR line Laser:
Use as second imaging
line/ laser or for
simultaneous stimulation(optional SIM scanner)

Automatic Introduction
Optic

Introduction optic with AOM attenuation(0% — 100%, 0.1% increment)
Including fully automated beam expander,
XY shifter and two axes angle alignment.
(4 Axes Quadralign Auto Alignment optic)
Direct coupling to laser port of scanning unit.

Twin Lasers System

INSIGHT X3 DUAL-OL
INSIGHT X3 DUALC-OL

MAITAI HPDS-OL
MAITAI eHPDS-OL
INSIGHT X3-OL
Chameleon Vision I Olympus
Chameleon Vision II Olympus
Chameleon Vision S Olympus

1045 nm fixed line from
INSIGHT X3 DUAL / DUALC-OL

MAITAI HPDS-OL
MAITAI eHPDS-OL
Chameleon Vision I Olympus
Chameleon Vision II Olympus
Chameleon Vision S Olympus

Introduction optic with 2 sets of AOM attenuation (0% — 100%, 0.1% increment)
Including 2 sets of fully automated beam expander, XY shifter and two axes anglealignment.
(4 Axes Quadralign Auto Alignment optic)
Direct coupling to laser port of scanning unit.
Motorized light path switcher, with DM900, DM1000R, DM1100 to combine two IR
wavelength for imaging.

IR laser combining optic

Scanning Unit

Dual Lines System∙ Spectra-Physics products :MAITAI HPDS-OL: 690 nm — 1040 nm
MAITAI eHPDS-OL: 690 nm — 1040 nm
INSIGHT X3-OL: 680 nm — 1300 nm
INSIGHT X3 DUAL/DUALC-OL: 680 nm — 1300 nm + 1045 nm∙ Coherent products:
Chameleon Vision I Olympus : 690 nm — 1040 nm
Chameleon Vision II Olympus : 680 nm — 1080 nm
Chameleon Vision S Olympus : 690 nm — 1050 nm

Optional Visible lightlaser for stimulation

405 nm/50 mW, 458 nm/20 mW, 588 nm/20 mW laser source with AOTF attenuation. 0% — 100%, 0.1% increment, < 2 µs rising time

Scanning Method

Light deflection via 2 silver-coated galvanometer scanning mirrors, or silver-coated resonant scanning mirror.

Scanning Speed

Galvanometer Scanner (Normal Imaging) : 512 × 512 with 1.1 s — 264 s. Pixel time : 2 µs — 1000 µs.
Resonant Scanner (High Speed Imaging) : 30 fps at 512 × 512, 438 fps at 512 × 32

Scanning Mode

XY, XYZ, XYT, XYZT, free line, XZ, XT, XZT, PointT

Galvanometer Scanner(Normal Imaging)

Galvanometer ROI scanning: Rectangle Clip, Ellipse, Polygon, Free Area, Line, FreeLine & Point.
Zoom: 1.0× — 50.0× with 0.01× increment, support 0° — 360° rotation and pan
Scanning Field Number: 18
Image Size: 64 × 64 — 4096 × 4096

Resonant Scanner(High Speed Imaging)Resonant ROI scanning: Rectangle Clip, Line.
Zoom: 1.0× — 8.0× with 0.01× increment
Scanning Field Number: 18
Image Size: 512 × 512

Optical Coating

IR support optic with 1600 Coating.

Non Descanned MPE
imaging detectors

Reflected detection: 2 or 4 channel configuration: 2 PMTs configuration, 4 PMTs configuration or 2 PMTs + 2 cooled GaAsP-PMTs
Transmitted detection: 2 PMTs unit with high NA condenser.

Transmitted lightdetector

Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation
to microscope frame

Z-Drive

Integrated motorized focus module of the microscope, minimum increment 0.01 µm
Optional: highly rigid piezo nosepiece*1

Optional Simultaneous
Stimulation ScannerHighly synchronized simultaneous stimulation scanner, including a set of Galvanometer Scanner, VIS and IR laser port.
ROI scanning: Rectangle Clip, Ellipse, Polygon, Tornado, Free Area, Line, FreeLine & Point.

Optional analog anddigital in out box

4 channels analog signal input, 6 channels digital TTL trigger input, 5 channel digital TTL trigger output. Scanner timing output

MPE Objective
FV30-AC10SV
with Auto Spherical
Aberration
Compensation Function FV30-AC25W

MAG.: 10X, NA: 0.6, W.D.: 8mm, Immersion Medium: SCALEVIEW-A2 (Water, Silicone oil and Normal oil available)

Operation Environment

Room temperature: 20 — 25°C, humidity: 75% or less at 25°C, requires continuous (24-hour) power supply

Size of Anti-vibration table

1500 mm × 1650* mm*1800 mm with Inverted Microscope System

SoftwareMAG.: 25X, NA: 1.05, W.D.: 2mm, Immersion Medium: Water
1500 mm × 1650* mm*1800 mm with Inverted Microscope System

1500 mm × 2000 mm

Basic Feature

Dark room matching GUI design. User arrangeable layout.
Acquisition parameter reload features. Hard disk recording capability, Adjust laser power and HV with Z-Stack acquisition.
Z-Stack with alpha blending, Maximum intensity projection, Iso-Surface rendering

IR laser controlling

Fully integrated IR laser wavelength control and Deep Focus Mode

Optional Motorized
Stage software

XY motorized stage control, Map image acquisition for easy target locating. Tiling acquisition and software image stitching.
Define multiple area for time lapse imaging.

Optional Mappingand Multiple point
stimulation software

Multiple point stimulation and data acquisition software. Mapping multiple point stimulation to generate reaction map.
Filtering feature to select points. Multiple point stimulation. Single or repeat stimulation.
Each point independent stimulation wavelength selection.

Optional Sequence
Manager

Advanced programmable software to define multiple imaging/stimulation tasks and execute by hardware sequencer.
Minimum gap 100 ms delay between tasks.

Optional Auto
Compensation software

Automatic spherical aberration compensation software.
Control of objective lens with auto spherical aberration compensation function. Auto adjustment of motorized correction collar to find thebest position at certain observation depth.
Auto adjustment of correction collar along with Z movement.

*1 Not available in some areas.

14

Image data on cover page: YFP-H mouse Brain of 20 weeks old treated by Scal eS
Courtesy of:Hiroshi Hama, Atsushi Miyawaki
Laboratory for Cell Function Dynamics RIKEN Brain Science Institute•is ISO14001 certified.
•is FM553994/ISO9001 certified.
• Illumination devices for microscope have suggested lifetimes.
Periodic inspections are required. Please visit our website for details.

www.olympus-lifescience.com• This product is designed for use in industrial environments for the EMC performance. Using it in a residential
environment may affect other equipment in the environment.
• All company and product names are registered trademarks and/or trademarks of their respective owners.
• Images on the PC monitors are simulated.
• Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer.

Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

Printed in Japan M1779E-012020