目 次（contents）【Special lecture】

Three dimensional structures of mitochondrial cristae using electron
tomography

Mun, J.-Y. et al

pp.1

Missing link between eukaryote and prokaryote

Kozuka,Y. et al

pp.1

Mitochondrial function and structure: gonadotropin stimuli increase amountof adrenodoxin in the cap-shaped mitochondria, and glutathione S-transferase
π translocates in mitochondrial innermembrane for protection against
oxidative stress

Izumi, S.

pp.2

Three dimensional analysis of the vacuolar structure in papilla cells during
self- and cross-pollination in Brassicaceae

Iwano, M. et al

pp.2

Micromorphology of needle epicuticular wax of pine species by electron
microscopy

Kim, K.-W. et al

pp.3

Use of ionic liquid for developing new techniques for electron microscopy

Kuwabata, S. et al

pp.3

Measurement of intercellular space of normal esophageal epithelium byelectron microscopy

Uhm C.-S. et al

pp.4

New generation SEM technology for biological samples using the on-line
digital image acquisition and processing as well as several methods for signal
detection

Oho, E. et al

pp.4

A novel staining method for thin sections and en bloc tissues of rat kidney
fixed with glutaraldehyde and osmium tetroxide using methanolic hafnium
chlorideOnoe, T. et al

pp.5

Platinum blue staining for transmission electron microscopy as an alternative
to uranyl acetate

Inaga, S. et al

pp.5

Application of high voltage electron microscopy in neuroscience research

Rhyu, I.-J.

pp.6

Structure and function of protozoan cytoskeletonsSuzaki, T.

pp.6

Three-dimensional architecture of actin parallel bundle within a microvillusof rat absorptive epithelial cell by electron tomography

Ohta, K.

pp.7

The cockroach midgut cell population is regulated by autophagy andapoptosis under starvation

Park, M.-S. et al

pp.7

Cryo-electron tomography of Vibrio flagellar hook-basal body with Zernike
phase-contrast transmission electron microscopy

Hosogi, N. et al

pp.8

Hing, H. L. et al

pp.8【Education lecture】

Networking and consultant lab for diagnostic electron microscopy ininfectious diseases

Live CLEM: A new approach for observing molecular dynamics in highresolution

Haraguchi, T.et al

pp.9

New melasma treatment by collimated low fluence Q-switched Nd:YAG lasermodify the 3D structure of melanocyte by subcellular selective
photothermolysis

Mun, J.-Y.et al

pp.9

The efficacy evaluation of tourmaline-ionized water in animal study

Qi, X.-F.et al

pp.10

The effect of electrolyzed reduced water on the melamine-related toxicity in
mice

Yoon, Y.-S.et al

pp.10

Immunohistochemical and ultrastructural features of neuroendocrine
differentiated carcinomas of the prostate

Hirano, D.et al

pp.11

CD40 membrane protein reconstitution into liposome membrane and thecellular uptake of reconstituted liposome

Lee, K.-E.et alpp.11

Pathological roles of reactive oxygen species in plant pathogen

Hyon, G.-S.et al

pp.12

Visualization of the mRNA structure in human cells by immuno-electron
microscopy

Ishigaki, Y.et al

pp.12

Three dimensional structure of Trichomonas vaginalis

Lee, K.-E. Y.et al

pp.13

The cytoarchitecture of the cardiac muscles in the human right ventricular
outflow tract

Yamaguchi, T.et al

pp.13

The organization of the epithelium of the lower rectum and the anal canal in
the adult humanTanaka, E.et al

pp.14

The structural organization of the lamina muscularis mucosae and lymphatic
vessels in the human stomach

Akashi, Y.et al

pp.14

Epidermal tight junction: the master skin barrier regulator

Sasaki, H.

pp.15

Artifacts are functionless structures which mislead the electron microscopic
evaluation in stressed biological tissues

Muranaka, Y.et al

pp.15

A speedy specimen preparation method of plant tissues infected withpathogens for scanning electron microscopy by using ionic liquid

Koga, H.et al

pp.17

A speedy specimen preparation method of Neotyphodium endophyte hyphae
in grasses for immuno-scanning electron microscopy by ionic liquid

Mizuno, S.et al

pp.17

A new sample preparation method using ionic liquid for scanning electron
microscopy in the field of bacteriology

Hotta, Y.et al

pp.18

The future of scanning electron microscope observation method using ionicliquid

Jike, T.et al

pp.18

New scanning electron microscopy of the cell and tissue by using the ionicliquidKawazato, H.et al

pp.19【Poster presentation】

The progression process of stack formation in the Golgi apparatus revealed byscanning electron microscopy

Naguro, T. et al

pp.19

Low vacuum SEM for rapid three-dimensional analysis of renal biopsy
sections

Inaga, S. et al

pp.20

3D-scanning electron microscopy with functions for image observation,
reconstruction and quantitative measurement

Oho, E. et al

pp.20

Quality improvement in SEM images of biological samples by using helium
gas under a low-voltage, low-vacuum environment

Yamazaki, S. et al

pp.21

Practical method for obtaining ultrahigh quality SEM images using complex
hysteresis smoothing and its application to biological fieldSuzuki, K. et al

pp.21

SBF-SEM. Serial block face imaging in the scanning electron microscope

Mancuso, J. et al

pp.22

Lower energy observation biological and soft-materials for the reduction ofbeam damage

Fujisawa, A. et al

pp.22

Relationship between different relative thickness, mean free path andmapping exactitude in Ca element analysis by using EF-TEM

Moriguchi, K. et al.

pp.23

Comparison of a non-invasive evaluation of liver fibrosis and histopathologic
and electron microscopic evaluation methods for NASH

Fukuzawa, Y. et al

pp.23

Electron computed tomography construction of the synapse with fiducial
marker free automated system

Oh, S.-H. et al

pp.24

Treatment of periodic acid may enhance the attachment ability between
specimen surfaces and the ambient resin during ultrathin sectioning

Shimoi, S. et al

pp.24

Water-involved resin blocks always give us bad thin-sections causing the poor
cell images of biological tissues

Meguro, H. et al

pp.25

A novel staining method of methanolic hafnium chloride (MHC) for uranyl
acetate; staining effect of MHC for thin sections of bacteria, fungi, humanbiopsies, rat tissues and plants

Horiuchi, Y. et al

pp.25

The usefulness of oolong tea extract in an electron staining with platinum
blueTakei, S. et al

pp.26

Hematoxylin as an alternative staining solution to uranyl acetate fortransmission electron microscopy

Arai, H. et al

pp.26

Preparation for nematodes for electron microscopy

Adachi, M. et al

pp.27

Oxygen-bubbly glutaraldehyde f ixation can preserve mucoproteins ormucopolysaccharides in the vacuoles of plant cells

Kitaoka, H. et al

pp.27

Extinction of membrane contrast with Sorensen phosphoric acid buffer
including potassium in the preparation of transmission electron microscopesamples

Ohta, I. et al

pp.28

Oxygen free radical production in human polymorphonuclear leukocytes
when fixed with glutaraldehyde

Moriguchi, K. et alpp.28

Ionic liquid is a powerful agent to observe the TEM ultrastructure of fixed or
unfixed biological materials

Nemoto, N. et al

pp.29

Morphological changes with time-course after sampling human platelet

Yokoyama, M.et al

pp.29

Recent role of electron microscopy in rapid diagnosis of emerging and reemerging infectious agents in Vietnam

Nguyen, T. T.et al

pp.30

Ultrastructural images reveal basidiospores development in an edible
mushroom, “shoro”

Shimomura, N.et al

pp.30

Taxonomic evolution of septal pore ultrastructure in the cantharellales(Basidiomycota, fungi)

Yanaga, K.et al

pp.31

Evaluation of the toxic effect of azoxystrobin on Botrytis cinerea

Park, P.et al

pp.31

The dynamics of reactive oxygen species generation during sclerotia
development in Sclerotinia minor

Ikeda, K.et al

pp.32

Mycelial incompatibility in Rosellinia necatrix

Inoue, K.et al

pp.32

Ultrastructural observation of mature and immature sheaths formed byLeptothrix ochracea

Suzuki, T.et al

pp.33

SEM and EDX analyses on the sheath of a strain of Leptothrix cholodnii
isolated from groundwater in Japan

Sawayama, M.et al

pp.33

Characterization of iron oxide sheath formed by iron oxidizing bacteria
Leptothrix ochracea

Furutani, M.et al

pp.34

Is a coccoid form of Helicobacter pylori a partial denaturing of the bacillaryform or is it simply a temporary defense mechanism to endure theunfavorable environment ?

Lee, S.-H.et al

pp.34

Complement-mediated bacteriolysis after binding of specific antibodies todrug-resistant Pseudomonas aeruginosa

Tanaka, J.et al

pp.35

Silver ions induced damage of Staphylococcus aureus observed by usingtransmission electron microscope

Kawakami, H.et al

pp.35

Electron microscopic study on antibacterial activity of silver nanoparticles

Tran, Q. H.et al

pp.36

Antibacterial effect of sealants containing TiO2 particle (in vitro and in vivo)

Nagame, S.et al

pp.36

Influence of oral bacteria, TW295, on cerebral hemorrhage in mice

Hokamura, K.et al

pp.37

Ultrastructural study on the indigenous bacteria in the lumen of rat intestine

Ito, E.et al

pp.37

Effect of Clonorchis sinensis infection on the mitochondrial enzyme of SD rat
bile duct fibroblast

Kim, S.-J..et al

pp.38

Pathogenicity of Staphylococcus aureus in different growth phase to mouse
kidneySeki, K.et al

pp.38

Effect of Clonorchis sinensis infection on the mitochondrial enzyme of SD rat
bile duct fibroblast

Kim, S.-J.

pp.39

The oogenesis of Rhynchocypris oxycephalus, Leuciscinae, Teleostei

Kim, D.-H. et al

pp.40

Ultrastructure of germ cells and the functions of leydig cells and sertoli cells
associated with spermatogenesis in Pampus argenteus (Teleostei,Perciformes, Stromateidae)

Yang, Y.-C. et al

pp.40

Sperm structure of the bastard halibut, Paralichthys olivaceus(Paralichthyidae, Pisces)

Lee, B.-C. et al

pp.41

Encapsulation of sericin into liposome and their application to culture
supplement for islet model system

Lee, K.-E.et al

pp.41

Ultrastructural change of Drosophila spermatogenesis in mip40 mutant

Park, S.-J. et al

pp.42

A C.elegans homolog of the human ATP synthase subunit e and g controls
cristae morphology in muscle cellMun, J.-Y. et al

pp.42

Microdistribution of copper and iron nanoparticles in wood treated with a
newly-developed wood preservative

Matsunaga, H. et al

pp.43

Ultrastructural observation of the compatible and incompatible pollen tube in
the style in S-homozygotes of the Japanese pear

Kono, Y. et al

pp.43

Three-dimensional analysis of intracellular organelles in a papilla cell ofBrassica rapa

Nagai, R. et al

pp.44

Ultrastructural analysis of the papilla formation during plant-microbe
interaction

Miyata, N. et al

pp.44

Generation of reactive oxygen species in necrotic leaves of crossed progeny
between tetraploid wheat and Aegilops tauschii

Mizuno, N. et al

pp.45

Abnormal chloroplasts observed in synthetic hexaploid wheat lines showing
hybrid chlorosis

Nakano, H. et al

pp.45

Non-mycorrhizal plant, Qing-Gng-Cai (Brassica rapa var. chinensis), canabsorbs directly humic substance, named PEON

Yoshida, T. et al

pp.46

Water transport in xylem elements in the parasitic interaction of host (Lotusjaponicas) and parasitic plants

Kubo, M. et al

pp.46

Observation of the protrusion structure on cell outer layer of halotolerant
microalga Dunaliella parva caused by the hyperosmotic stimulation

Hatanaka, Y. et al

pp.47

Effect of cyclosporin A and nifedipine on the amiodarone-induced
phospholipidosis in the liver of rats

Yamamoto, K. et al

pp.47

Effects of red wine on the brown adipose tissue and fatty liver

Wang, H.-P. et al

pp.48

Morphological diversity of mitochondria in some cultured cell types- High voltage electron microscopic analysis of whole mount cells -Kim, H.-W. et al

pp.48

Observation of tight junction-like structure by HAADF-STEM tomographyand quick-freeze-fracture-replica

Kato, T. et al

pp.49

Basic fibroblast growth factor induced rat bone marrow mesenchymal stem
cells differentiate into cardiomyocyte-like cells in vitro

Wang, H.-P. et alpp.49

Effects of sensory stimulation on neurogenesis in the barrel cortex after focal
ischemic stroke in adult rats

Kim, S.-H. et al

pp.50

Ultrastructural analysis of osteoblasts, osteocytes and odontoblasts in Runx2
transgenic mice

Miyazaki, T.et al

pp.50

ED1 antibody reactivity of osteoclasts in the alveolar bone around rat incisors
shown by pre-embedding immunoelectron microscopy

Nishikawa, S.

pp.51

FE-SEM observation of surface changes following treatment with surfactant
in human T and B lymphocytes

Kono, M. et al

pp.51

A selective inhibitor of macrophage, 2-chloadenosine induce necrosis ofmouse macrophage

Saito, T. et al

pp.52

Ultrastructure of fatty components in human milk

Yasuda, A. et al

pp.52

Ultrastructural and immunohistochemical study on the apoptotic process ofintestinal epithelial cells in rats

Nishida, M. et al

pp.53

Ultrastructural and histoplanimetrical demonstration of direct transportationof chylomicrons via subepithelial blood capillaries in rat intestinal villi

Takahara, E. et al

pp.53

Scanning electron microscopic study on the persorption mechanism of large
antigenic particulates from small intestinal lumen in rats

Fukui, Y. et al

pp.54

Immunohistochemical study on the Toll-like receptor expression in villous
columnar epithelial cells of rat small intestine

Mantani, Y. et al

pp.54

Communication routes between the epithelium and lamina propria mucosae
in the human colon

Nagai, K. et al

pp.55

Three dimensional observation of the connective tissue papillae of humanoral mucosa

Asai, S. et al

pp.55

Effect on the human epidermis by glycolic acid peeling

Chang, B.-S. et al

pp.56

Activin A and eCG recover reproductive dysfunction induced by neonatal
exposure to an estrogenic endocrine disruptor in adult male mice

Warita, K. et al

pp.56

Localization and developmental fate of fetal Leydig cells in mouse testis

Yokoyama, T. et al

pp.57

The spermatogenesis of Coreoleuciscus splendidus, Gobioninae, Cyprinidae,
TeleosteiTeng, Y.-C. et alpp.57Comparative study on morphology of Malaysian cow sperms preserved using
high pressure freezing and freezing substitution as compared to sperms addedwith coenzyme Q10Hing, H. L. et al

Cytoskeletal dynamics in the bovine oocytes following nuclear transferredoocytesKim, N.-H. et al

Chondrosarcoma with myxoid change; a study using quick-freezing anddeep-etching method
Comparative examination of electron microscopic feature withhistopathologic stage of NASH

pp.58

pp.58

Hemmi, A. et al
pp.59

Ohji, M. et alpp.59

Effects of bacillus calmette-guerin (BCG) on the DNA synthesis of mouse
duodenal mucosal epithelial cells inoculated with Ehrlich carcinoma cells

Ko, J.-S. et al

pp.60

Human aorta and arteries as revealed by light microscopy and scanningelection microscopy

Zhang, H. et al

pp.60

Interpretation of cytotoxicity of nano-sized ferric oxides with different shapes

Choi, E.-K. et al

pp.61

Analysis of the ultrastructures of four light-cure orthodontic composite-resins

Scougall-Vilchis,R. J. et al

pp.61

6th ISEM 2009 Commemorative Photo【学会通信】【投稿規定】【表紙】

写真説明：クローディン３発現マウスL細胞の急速凍結割断レプリカで、GA固定、FEI社製TecnaiG2F20（加速電圧200kv）備え付けのHAADF-STEMで行いました。
図中のbarは200nmです。