実 験 動 物 ニュース
The Japanese Association for Laboratory Animal Science

目

次

他学会情報

ICLAS 情報....................................................................................................................... 1

The Guidelines of International Nomenclature................................................................... 3
EXPERIMENTAL ANIMALS 57(1) 収載論文和文要約集........................................ 5

日本実験動物学会正会員名簿の変更一覧........................................................................i
維持会員名簿.........................................................................................................................ii

Vol. 57 No. 1 / January 2008

実験動物ニュース他

学会

ICLAS 情報1．関連学会，講習会等の案内

a. NABR’s 2008 Leadership Conference andInternational Forum, Communication &Collaboration: An International Forum for
Animal Research Policy

June 23–25, 2008, Washington, DC

F o r m o r e i n f o r m a t i o n , s e e h t t p : / / w w w.
animalresearchforum.org/.

b. Alternatives and 3Rs Workshop

January 24–25, 2008 in Los Angeles, sponsored

by UCLA and the Johns Hopkins Center for
Alternatives to Animal Testing (CAAT). Objectiveof the workshop is to learn about new scientificinnovations and ethical perspectives in research
animal alternatives and the “3Rs”. Please contact

Kathy Wadsworth at arc@oprs.ucla.edu if you
request additional information.

c. ACLAM Foundation

The ACLAM Foundation Committee members arepleased to announce our solicitation of research

proposals in Laboratory Animal Science andMedicine.

For more information, go the aclam foundation
website:http://aclam.org/foundation/rfp.html
or contact:

Dr. Gregory P Boivin

Scientific Director, ACLAM Foundation
University of Cincinnati

P.O. Box 670529 Cincinnati, OH 45267-0529
Phone: 513-558-9156

E-mail: boivingp@ucmail.uc.edu

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情

報

d. 29th World Veterinary Congress
July 27–31, 2008

Vancouver, Canada

S u p p o r t e d & P r o m o t e d b y C V M A , Wo r l dOrganization for Animal Health and World
Veterinary Association<http://www.meet-ics.com/wvac2008/support/footer.
jpg>

CONGRESS SECRETARIAT: International
Conference Services Ltd., Suite 2101-1177 West
Hastings Street, Vancouver, BC Canada V6E 2K3Phone: (604) 681 2153 Fax: (604) 681 1049
E-mail: wvac2008@meet-ics.com

Privacy Policy <http://www.meet-ics.com/ics_priva
cy_policy_june_2004.pdf>
2．ニュースDr. Joe Held 逝去のお知らせ

ICLAS is saddened to announce the death of

Dr. Joe Held.

Dr. Held was an inspiring leader inlaboratory animal science in North and South America
and throughout the world. His contributions to theadvancement of laboratory animal science in Latin
America were significant.

He was instrumental indeveloping laboratory animal resources at the U.S.
National Institutes of Health and extended thoseresources to the activities of the PanAmerican HealthOrganization. Among his many contributions, he
was chairman of the International Scientific Advisory
Board of the Institute for Primate Research, National

Museums of Kenya. He chaired the committee of theCouncil for International Organizations of Medical
Sciences (CIOMS) responsible for drafting CIOMS’s“International Guiding Principles for Biomedical



実験動物ニュース

Research Involving Animals”.

He and Dr. Tatsuji

Nomura instituted the US-Japan meetings, held
annually to discuss salient issues in the use and qualityof animals required for research.

Dr. Held was a friend of ICLAS and we honor his

many contributions throughout the world. We send oursincerest condolences to his wife and family.
3．出版(2004) Guidelines for the Use of Fishes in Research.

These are available at the American Fisheries Society.

web page: http://www.fisheries.org/afs/publicpolicy/

guidelines2004.pdf
4．ICLAS 会議

a. ICLAS International Consortium Meeting

2007年10月16日，
米国North CarolinaのCharlotte

で AALAS と合同の上記会議が開催されました。
その議事録によりますと，
参加者は12カ国，25名，日本からは黒澤先生が参加されました。その主要議題を以下に示します。

1. New ICLAS Governing Board (2007–2011)

2. Harmonization of Guidelines update.
3. Reciprocal Registration

4. ICLAS Network for the Promotion of Animal

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Quality in Research update

5. Americas Committee update

6. ILAR Update of the Guide for the Care and Useof Laboratory Animals

7. Association for the Assessment and Accreditationof Laboratory Animal Care International(AAALAC) update.

8. Announcements and Future Meetings

b. ICLAS FYI Bulletin

ICLAS FYI Bulletin の受信者を更新中です。受信希望者は氏名，メールアドレスを送ってください。

I am in the process of updating the list of recipientsof the ICLAS FYI Bulletin. Please let me know if
you wish your name to be removed or if you would

like to have individuals added. Please send me names
and email addresses if you wish to add colleagues tothe list.

Steven P. Pakes, DVM, PhD

Professor of Pathology, UTexas Southwestern Med.
Ctr.

5323 Harry Hines Blvd. Dallas, TX 75390-9072
E-mail: steven.pakes@UTSouthwestern.edu
Phone: 214-648-1684 Fax: 214-648-4096
URL: http://www.iclas.org

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The Guidelines of International Nomenclature
The information of animals, strains, genes, etc, appearing in papers submitted to the official journal
Experimental Animals, should be given according to the rules and guidelines of international nomenclature.
For this reason, authors are required to implement the nomenclature. The editorial board advises authors
to refer to the information below, when preparing a manuscript for submission to Experimental Animals for
publication.
The current nomenclature applied to mice and rats follows the rules and guidelines established by theInternational Committee on Standardized Genetic Nomenclature for Mice. This nomenclature will beapplied to other experimental animals, too.

1. Scientific name of experimental animals
The scientific name of animals used in animal experimentations should be given.
Examples:a. Mouse: Mus musculus musculusb. Rat: Rattus norvegicus

2. Breeder’s name or Institute’s name
The official title of the Breeder or Institute from which the experimental animals originated or werepurchased should be given.
a. Use of “in-house” or “domestic”, is recommended, if the animals originate from the author’s
animal facility.
b. The official title should be referenced from the home page or brochure (pamphlet) issued by the
Breeder or Institute.

3. Strain name(1) Names of strains distributed by commercial breeders should be referenced from the Breeder’shome page or brochure (pamphlet).
(2) Names of newly bred strains should be given according to international nomenclature andinternational index of experimental animals.
Home page of Rules and Guidelines for Nomenclature of Mouse and Rat Strains:
http://www.informatics.jax.org/mgihome/nomen/strains.shtml
http://rgd.mcw.edu/nomen/nomen.shtml#StrainNomenclature
Home page of the Index of Major Mouse Strains:
http://www.informatics.jax.org/external/festing/mouse/STRAINS.shtml
Home page of the Index of Major Rat Strains:
http://www.informatics.jax.org/external/festing/rat/STRAINS.shtml
http://rgd.mcw.edu/strains/

4. Genes, Genetic Markers, Alleles, and Mutations
Scientific and formal names of genes and proteins should be found in Mouse Genome Informatics(MGI) and Rat Genome Database (RGD).
MGI home page: http://www.informatics.jax.org/mgihome/nomen/gene.shtml
RGD home page: http://rgd.mcw.edu/nomen/nomen.shtml#StrainNomenclature

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5. Laboratory code (Lab code)
A Laboratory Code is a code of usually one to four letters (first letter is upper case, followed by lower
case letters), that identifies a particular institute, laboratory, or investigator that produced, and may
hold stocks of a DNA marker, an animal strain, or a mutation. Laboratory codes are also used innaming chromosomal aberrations and transgenes.
Examples:(1) C3H/HeH: Mouse substrain derived at Harwell (H) from the Heston (He) substrain of C3H(2) D8Mit17: A DNA segment that has the 17th locus mapped to mouse Chromosome 8 by MIT
Institute for Laboratory Animal Research (ILAR)
Home page for search: http://dels.nas.edu/ilar_n/ilarhome/search_lc.php
Home page for registry: http://dels.nas.edu/ilar_n/ilarhome/register_lc.php

6. Miscellaneous(1) Full translations and comprehensive explanations of nomenclatures translated to Japanese areavailable at the following address.
http://cardb.cc.kumamoto-u.ac.jp/transgenic/index.jsp(2) “Godfather” as a naming tool is available at the following address for learning nomenclatures.
http://www.shigen.nig.ac.jp/tools/godfather/index.jsp?lang=japaneseNote
The implementation of the nomenclature is carried out at the following databases, MGD (Mouse
Genome Database), IMSR (International Mouse Strain Resources), CARD (Center for Animal Resource
and Development at Kumamoto University), BRC (RIKEN BioResource Center), EMMA (European Mouse
Mutant Archive), RGD (Rat Genome Database) and NBRP-Rat (National Bio Resource Project for the Rat)
through each Genetic Nomenclature Committee.

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Experimental Animals—和 文 要 約—Vol. 57, No. 1 January 2008

原著

不活化狂犬病ウイルスの経鼻免疫による狂犬病発症防御免疫誘導.....................................1–9
米田篤史 1)・土屋耕太郎 2)・高島康弘 3)・新川 武 4)・辻 尚利 5)・
林 良博 1)・松本安喜 1)
東京大学大学院農学生命科学研究科国際動物資源科学研究室， 2) 日生研， 3) 岐阜大学応用生物科
学部獣医寄生虫学分野， 4) 琉球大学遺伝子実験センター， 5) 独立行政法人農業・生物系特定産業技術研究機構動物衛生研究所寄生虫病学研究室

1)

低侵襲性の免疫法として有望である経鼻免疫による狂犬病発症防御免疫誘導の可能性について検討した。経鼻免疫抗原には，不活化狂犬病ウイルスを超遠心濃縮したもの（CRV）を用いた。CRV 30 m l に粘膜免疫アジュバントであるコレラ毒素（CT）5 m g を添加し，6 週齢雌ddY マウスに 5 回経鼻投与した。また，対照群として，CT 非添加経鼻免疫群，および同量のCRV を 2回腹腔免疫した群を設定した。CT添加経鼻免疫群の抗原特異的IgG 抗体価は腹腔免
疫群と同程度であった。また，CT 添加経鼻免疫群の狂犬病ウイルス RC-HL 株に対する中和抗体価は，腹腔免疫群より若干低値を示した。一方，CT 非添加経鼻免疫群の特異的 IgG 抗体価
および中和抗体価は，前述の 2 群よりも有意に低値であった。さらに，CT 添加経鼻免疫群と腹腔免疫群では，特異的 IgG2a 抗体価が高値を示し，Th1 細胞の活性化が示唆された。免疫マウ
スに致死量の狂犬病ウイルス CVS 株を接種したところ，CT 添加および CT 非添加経鼻免疫群のそれぞれ 67% および 17% が生残し，腹腔免疫は全頭生残した。生残マウスと死亡マウスの間には特異的IgG および IgG2a 抗体価，中和抗体価に有意な差が認められた。これらの結果は，不活化狂犬病ウイルスを用いた経鼻免疫による狂犬病発症防御免疫の誘導が可能であること，
さらに CT の添加によりその効果が増強されることを示唆している。

Licorice Extract Does Not Impair the Male Reproductive Function of Rats................. 11–17
Sunhee SHIN1), Ja Young JANG1), Byong-il CHOI1), In-Jeoung BAEK1), Jung-Min YON1),
Bang Yeon HWANG2), Dongsun PARK1), Jeong Hee JEON1), Sang-Yoon NAM1),
Young Won YUN1), and Yun-Bae KIM1)
1)

College of Veterinary Medicine and Research Institute of Veterinary Medicine, 12 Gaeshin-dong,
Cheongju, Chungbuk 361-763, and 2)College of Pharmacy, Chungbuk National University,
12 Gaeshin-dong, Cheongju, Chungbuk 361-763, Korea

The effect of water extract of licorice (Glycyrrhiza uralensis), one of the most widely used
medicinal plants in Oriental nations and in Europe, on male reproductive function was investigated inrats. Licorice extract was prepared as in Oriental clinics and orally administered at doses of 500, 1,000
or 2,000 mg/kg, the upper-limit dose (2,000 mg/kg) recommended in the Toxicity Test guideline of the

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Korea Food and Drug Administration, to 6-week-old male rats for 9 weeks. Licorice extract neither
induced clinical signs, nor affected the daily feed consumption and body weight gain. There were no
significant changes in testicular weights, gross and microscopic findings, and daily sperm production
between vehicle- and licorice-treated animals, in spite of slight decreases in prostate weight and daily
sperm production at the high dose (2,000 mg/kg). In addition, licorice did not affect the motility andmorphology of sperm, although the serum testosterone level tended to decrease without significant
difference, showing a 28.6% reduction in the high-dose (2,000 mg/kg) group. The results suggest thatthe no observed adverse-effect level of licorice extract is higher than 2,000 mg/kg, the upper-limit
dose, and that long-term exposure to licorice might not cause profound adverse effects.

Effect of Maternal Restraint Stress on Fetal Development of ICR Mice..................... 19–25
Yea Eun LEE1), Sang Kuk BYUN1), Sunhee SHIN1), Ja Young JANG1), Byong-il CHOI1),
Dongsun PARK1), Jeong Hee JEON1), Sang-Seop NAHM2), Jong-Koo KANG1),
Seock-Yeon HWANG3), Jong-Choon KIM4), and Yun-Bae KIM1)
1)

College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National
University, 12 Gaeshin-dong, Cheongju, Chungbuk 361-763, 2)College of Veterinary Medicine, Konkuk
University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, 3)Department of Clinical Laboratory
Science, Juseong College, San 4 Deokam-ri, Naesu-eup, Cheongwon, Chungbuk 363-794, and4)
College of Veterinary Medicine, Chonnam National University, 300 Yongbong-dong, Buk-gu,
Gwangju 500-757, Korea

The present study was conducted to elucidate the susceptibility of embryos and fetuses at different
gestational stages to the maternal stress in mice. Groups of pregnant ICR mice were subjected todaily 12-h restraint stress, taped in the supine position on a plastic board, on gestational days (GD) 1–4,
5–8, 9–12 and 13–16, respectively. Caesarean sections were performed on gestational day 18, and thefetuses were weighed and examined for morphological defects. During the daily restraint for 4 days,
the maternal body weights markedly decreased. Although the body weights recovered gradually aftertermination of the stress, the recovery was not full until the final stage of pregnancy. Interestingly,
restraint stress caused growth retardation of the fetuses, leading to a significant decrease in theirbody weights, and increased early and late resorptions of embryos and fetuses according to the stressperiods. Although the preceding (GD1–4) and concurrent (GD5–8) stresses did not affect embryonic
implantation, restraint stress on GD9–12 caused cleft palate. Whereas vertebral abnormalities, mainly
bipartite ossification, were observed only in animals stressed on GD5–8, abnormalities of sternebrae,
exhibiting asymmetric or bipartite ossification, were enhanced by the stress at all of the gestational
stages. On the other hand, the incidence of other malformations including renal malposition andcostal abnormalities was not increased by stress at any of the 4 stages. Taken together, the results
suggest that intensive restraint stress influences the maternal body weight resulting in growth
retardation and increased mortality of embryos and fetuses, in addition to gestational stage-specificventricular dilatation, cleft palate and sternal abnormalities.

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The Effect of Progesterone and Exogenous Gonadotropin on Preimplantation Mouse
Embryo Development and Implantation.................................................................. 27–34
Soraya Rasi GHAEMI, Mojdeh SALEHNIA, and Mojtaba Rezazadeh VALOJERDI
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

The aim of this study was to evaluate the effects of progesterone and ovarian stimulation on thedevelopment and implantation rate of mouse embryos. Two-cell embryos were collected fromsuperovulated mice and cultured in the presence of different concentrations of progesterone (0, 5, 10
and 20 ng/ml). Also other mice were rendered pregnant in unstimulated, unstimulated progesteroneinjected, superovulated and superovulated progesterone-injected groups to collect the blastocysts.
The number of blastocysts and implantation sites were recorded on the 4th and 7th day of pregnancy,
respectively. The diameter and cell number of blastocysts were analyzed in the in vitro and in vivogroups. After 120 h culture, the percentage of hatched blastocyst embryos in control and 5, 10 and
20 ng/ml progesterone-injected groups were 63.9%, 64.2%, 64.2% and 75.6% respectively. There
were significant differences between the developmental rates of embryos in the presence of 20 ng/ml
progesterone and the control and other concentrations of progesterone-injected groups (P≤0.001).
The in vivo blastocyst survival rate (97.68%) and implantation rate (92.06%) in the unstimulated
and progesterone-injected groups were higher than in the other groups. Blastocyst cell numbers inthe superovulated (128.62 ± 1.30) and superovulated progesterone-injected groups (126.88 ± 1.60)
were significantly different from the control (P<0.001). The progesterone injection without ovarian
induction improved the embryo survival and implantation rates, but after superovulation it did notameliorate the negative effects of superovulation on the implantation rate.

雌雄マウスの免疫反応に対するメタンフェタミン投与回数の影響............................... 35–43
斎藤正好 1, 4)・寺田 賢 2)・川田哲也 1)・伊東久夫 1)・茂松直之 3)・.
プチャラポーン クロムカン 4)・横須賀 誠 4)・斎藤 徹 4)1)

千葉大学大学院医学研究院放射線医学，2) 東邦大学医学部法医学，3) 慶應大学医学部放射線腫瘍・
核医学科， 4) 日本獣医生命科学大学獣医学部比較動物行動医学

メタンフェタミン（methamphetamine：MAP）の投与回数が免疫反応におよぼす影響について雌雄マウス（ddY）を用いて検討を行った。マウスの腹腔内にMAP 5 mg/kg/日を1，5および10回の投与を実施し，白血球数，脾リンパ球のマイトゲン反応（phytohemagglutinin：PHA,

lipopolysaccharide：LPS）およびnatural killer（NK）細胞活性について検討を加えた。また，雌
雄マウスの脾臓内MAP濃度とその代謝過程で産出されるアンフェタミンの濃度を経時的に測定した。その結果，MAP の単一ならび複数回投与で雌雄ともに白血球数の減少が認められた。
PHAに対する反応では，単一投与で雄のみに明らかな上昇が認められ，LPS反応では雌雄とも僅かな上昇がみられた。NK 活性では雌雄とも活性の低下がみられ，特に雌では 5 回の投与後に活性が消失した。しかし，10回の投与後にNK活性と白血球数は対照群のレベルまで回復する傾向がみられた。脾臓内のMAP代謝は雌で遅延する傾向が認められた。これらの結果より，
MAP の免疫機能に対する影響は，投与量のみならず投与回数や雌雄によっても変化する可能性が示唆された。このような免疫反応に対する MAP の基礎的な検討は，中毒者の免疫系の変化を理解する上で必要であると思われる。

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Experimental Autoimmune Encephalomyelitis (EAE) Induced by Antigen Pulsed
Dendritic Cells in the C57BL/6 Mouse: Influence of Injection Route.....................45–55
Nasser AGHDAMI1), Farhad GHARIBDOOST2), and Seyed-Mohammad MOAZZENI1)
1)

Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, andRheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran2)

Dendritic cells (DCs) are the most potent antigen-presenting cells (APC) of the immune system,
and are critically involved in initiation of immune responses in autoimmune diseases. They canmodulate the nature of immune responses to stimulatory or tolerogenic fashion. Previous studies
have demonstrated that the administration route of DCs is an important variable in eliciting antitumor immunity. In this study we used experimental autoimmune encephalomyelitis (EAE) as an
animal model of multiple sclerosis to compare different protocols of DC delivery in autoimmunity
or tolerance induction. Dendritic cells were generated from bone marrow cells of C57BL/6 mice by
culturing in the presence of GM-CSF and IL-4 for 7 days, followed by 2 days culture with TNF-alpha.
The obtained DCs were pulsed in vitro with myelin oligodendrocyte glycoprotein (MOG) peptide andinjected (5x105 cells/mouse) via the intravenous (i.v.), intraperitoneal (i.p.) or subcutaneous (s.c.) route
into female C57BL/6 mice. In some instances pertussis toxin was also injected zero and 48 hours after
DC injection. After follow up of the mice pretreated in this way for 4 weeks, in the i.v. group in which
no clinical signs of EAE occurred, the mice were immunized with MOG peptide for EAE induction
via the common method and the results were compared with mice that were not pre-immunized.
Only after three s.c. DC injections with pertussis toxin, the mice showed mild clinical signs of EAE,
whereas mice given i.v. or i.p. injections with or without pertussis toxin failed to develop EAE after
4 weeks. Induction of EAE via the common method after three injections of TNF-alpha treated DCs,
in i.v. injected groups showed no protection from EAE. It seems that several factors influence thetolerance versus immunity induction by DCs. Our results showed that the administration route of DCs
is one of the pivotal factors in DC-based induction of autoimmune diseases.

Pasteurella pneumotropica とＶ因子要求性 Pasteurella 科菌の.

F344-rnu ラットを用いた感染実験........................................................................................ 57–63
林元展人・保田昌彦・植野昌未・後藤一雄・高倉 彰
財団法人実験動物中央研究所 ICLAS モニタリングセンター

P. pneumotropica（Pp）とラットから分離された V 因子要求性 Pasteurella 科菌（VFDP）の免
疫不全ラットに対する病原性を調査するためにそれぞれの菌株3株（ATCC 35149，CNP 160，
RPZ）と4株（V6–V9）を用いF344-rnuラットに対し感染実験を行った。4匹1群の動物に各株菌液を二回（0 日目，14 日目）経鼻接種し，初回接種後 60 日，120 日に剖検，細菌学的および組織病理学的検索を行った。Pp株を接種した動物では31日目までにATCC 35149株とCNP 160株を接種したそれぞれ数匹の動物にくしゃみの症状が観察された。細菌学的検索では 60 日
目に主に鼻腔，気管，120 日目に鼻腔，気管，肺から菌が分離された。組織病理学的検索ではATCC 35149株とCNP 160株を接種した全ての動物の鼻粘膜上皮に炎症・壊死像が観察された。
RPZ株を接種した動物からは臨床上の異常症状も組織病理学的な異常所見も観察されなかった。VFDP株を接種した動物の細菌学的検索では60日目に主に気管，120日目に気管と肺から菌が分離されたが，全ての動物で臨床上異常な症状と組織病理学的に異常な所見は観察されなかった。その結果，Pp は株により免疫不全ラットへの病原性が異なる事が示された。また
VFDP はラットの呼吸器に寄生する非病原性の細菌だと思われた。

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繰り返しテイルフリックテストにおける Isoflurane 浅麻酔と.

放射線強度設定の必要性.......................................................................................................... 65–72
高杉嘉弘・冬田昌樹・杉浦順子・薮田浩一・岩元辰篤・古賀義久
近畿大学医学部麻酔科学教室

テイルフリック反射は脊髄反射と考えられているが，上位中枢からの影響も受けている。テ
イルフリックテストは痛覚の測定にもっとも用いられている方法であるが，熱源の特性と繰り返し試験における測定値の信頼性について記述された報告は少ない。本研究では，上位中枢からの影響を抑制する浅麻酔下におけるテイルフリックテストでの有用性とテイルフリック潜時に影響を及ぼす放射熱の皮膚面温度への経時的効果について検討した。はじめに0，0.5，1.0，
1.5％イソフルラン吸入麻酔下での1時間の繰り返しテストにおける潜時への影響について検討し，つぎに熱刺激装置の赤外線（IR）放射熱量の特性を測定し，非線形近似式によって尾皮膚温の変化を推定し，再現性を得るための設定について検討した。この結果，吸入麻酔は濃度依存性にテイルフリック反射を抑制した。また IR 放射熱量は 2.5 秒以後に一定になり，皮膚温は最大IR放射熱量と放射時間に依存した。1.0％イソフルラン吸入ならびに10秒後に65℃となる161.5 mW/cm2 の放射熱量による刺激強度によって再現性のある潜時が得られた。本研究の結果は，テイルフリックテストにおいて再現性のある潜時を得るために浅い吸入麻酔は有用であり，熱刺激装置の特性を考慮する必要があることを示した。

短報

マウス第 15 染色体における組換えの抑制から明らかにされた Koala（Koa）.

および hairy ears（Eh）突然変異に関連した染色体逆位の領域................................... 73–77
片山健太郎・古野亜紀・宮本沙也加・中村美有紀・小鹿 泉・.
新海雄介・秋山耕陽・辻 岳人・国枝哲夫
岡山大学大学院自然科学研究科

Koa および Eh は，ともに第 15 番染色体上のほぼ同じ領域の逆位に起因するマウス突然変異であり，その表現型には耳介の被毛と頭蓋顔面の形成異常等の類似した部分が認められている。そのため両突然変異個体の表現型は逆位により生じた同一の遺伝子の変異に起因している可能性が考えられた。連鎖解析により組換え抑制領域を特定することで逆位領域を推定したところ，それぞれの逆位領域は異なっていることが判明した。そのためKoaおよびEhの表現型は異なる遺伝子によると推測された。

クロム化合物の Eimeria piriformis オーシスト芽胞化に対する影響............................... 79–83
李 明憲 1, 2)・黄 鴻堅 3)1)

国立中興大学獣医微生物学研究所，2) 中台科技大学医学検験生物技術系，3) 国立中興大学獣医学系

ウサギの糞便に排泄したばかりの未芽胞化（unsporulated）Eimeria piriformisオーシストを各濃度（1％，2.5％，5％および 10％）の各クロム化合物（K2Cr2O7，CrO3，Cr(NO3)3，K2CrO4）に処理した後，その芽胞化（sporulation）の程度を観察した。1から10％のK2Cr2O7 で処理したオー
シストは28時間でほとんどのものが芽胞化した。しかし，2.5％のCrO3 及びCr(NO3)3 で処理したオーシストが完全芽胞化するのに約 60 時間を要した。また，蒸留水，1％及び 10％ K2CrO4で処理したオーシストの完全芽胞化には，それぞれ216，156及び96時間を要した。よって E.
piriformis オーシストを芽胞化させるのに K2Cr2O7 が最適であることが明らかである。

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