The 71st Annual Meeting of
the Japanese Association forLaboratory Animal Science
ProgramProgram

The 71st Annual Meeting of the Japanese Association for Laboratory Animal Science
May 30 (Thu)

13:30 〜 15:30

Room 1 (ROHM Theatre Kyoto 1F South Hall)■ Honorable Contribution Award
Dr. Toshiaki Ochiai (Hamri Co., Ltd.)Dr. Yasuko Yamada (National Institute of Infectious Diseases)■ Ando-Tajima Award
L-1

Ichiro Miyoshi (Tohoku University)

Research on genome editing and animal models in experimental animal science○ Tomoji Mashimo
The University of Tokyo Institute of Medical Science■ Encouragement Award
L-2

Ichiro Miyoshi (Tohoku University)

Development of adriamycin nephropathy model and podocyte injury model using TRECK
method○ Masaki Watanabe
Lab. of Laboratory Animal Science and Medicine, Dep. of Veterinary Medicine, Kitasato Univ.

■ 2023 Best Paper Award of Experimental Animals
Rescue of oocytes recovered from postmortem mouse ovaries
Nagi Fujii, Yuta Nakata, Yoko Kato
Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- andsex-dependent manner
Masayuki Mori, Jian Dai, Hiroki Miyahara, Ying Li, Xiaojing Kang, Kazuto Yoshimi,
Tomoji Mashimo, Keiichi Higuchi, Kiyoshi Matsumoto

Establishment of a human microbiome- and immune system-reconstituted dual-humanized mousemodel
Yuyo Ka, Ryoji Ito, Ryoko Nozu, Kayo Tomiyama, Masami Ueno, Tomoyuki Ogura, Riichi Takahashi— 84 —

May 29 (Wed)

16:30 ～ 17:30

Room 4 (Miyako Messe B1F Main Conference Room)■ International Award

O-44

Kazuo Goto (Teikyo University)
Dinh Thi Huong Tra (RIKEN BRC)

Therapeutic potential of fenugreek seed extracts on Escherichia coli urinary tract infection in
BALB/c mice
○ Siti Nor Hikmah Abdul Rasid , Leslie Thian Lung Than , Pike See Cheah , Shalini Vellasamy
1)

1)
2)

O-45

1)

1)

2)

Faculty of Medicine and Health Sciences, Universiti Putra Malaysia,
Faculty of Medicine, Bioscience and Nursing, MAHSA University

Cognitive and social behaviors during abstinence from chronic toluene inhalation in adolescentrats○ Joannes Luke B. Asis , Johanna C. Munar , Ajina C. Carampel , Jariel N. Bacar ,3)
2)
2)
Cynthia Grace C. Gregorio , Gregory J. Quirk , Rohani Cena-Navarro
1)

2)

1)

2)

1)

College of Medicine, University of the Philippines Manila,
National Institute of Molecular Biology and Biotechnology, National Institutes of Health,
University of the Philippines Manila,
3)Institute of Chemistry, University of the Philippines Diliman
2)

O-46

Increased amyloid burden precedes glial activation in a genetic mouse model of Alzheimer’sdisease○ Yuek Ling Chai , Wei Wang , Jasinda H Lee , Christopher LH Chen , Thiruma V Arumugam ,1,2)
Mitchell KP Lai
1,2)

1)

3,4)

1,2)

5,6)

1)

Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore,
Memory, Ageing and Cognition Centre, National University Health System, Singapore,3)
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore,
4)
NUS Healthy Longevity Translational Research Programme, Yong Loo Lin School of Medicine,National University of Singapore,5)
Department of Physiology, Anatomy and Microbiology, La Trobe University, Australia,
6)
School of Pharmacy, Sungkyunkwan University, Republic of Korea
2)

O-47

Evaluation of phytochemical constituents, antioxidant capacity with special reference tohypoglycaemic activity of the tuberous root of Asparagus racemosus on Wistar albino rats
○ Anusha Senevirathne , Vijitha Paheerathan , Piratheepkumar Rajadurai , Sivakanesan Ramiah
1)

2)

2)

3)

1)

Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura,
Faculty of Siddha Medicine, Trincomalee Campus, Eastern University,
3)
Department of Biochemistry, Faculty of Medicine, University of Peradeniya
2)

O-48

Simultaneous protective immune responses of ducks against duck plague and fowl cholera byrecombinant duck enteritis virus vector expressing Pasteurella multocida OmpH gene
○ Nisachon Apinda , Venugopal Nair
1)

1)
3)

2,3,4)

1), Nattawooti Sthitmatee
2)

Faculty of Veterinary Medicine, Chiang Mai University, The Pirbright Institute,4)
Jenner Institute, University of Oxford, Department of Biology, University of Oxford— 85 —

Plenary Lecture 1 May 29 (Wed) 13:30 〜 14:30 Room 1 (ROHM Theatre Kyoto 1F South Hall)
Chairperson: Gen Kondoh (Kyoto University)

PL-1

Mechanism and in vitro reconstitution of mammalian germ-cell development○ Mitinori Saitou

1,2,3)

1)

Institute for the Advanced Study of Human Biology, Kyoto University Institute for Advanced Study,
Kyoto University,2)
Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University,
3)
Center for iPS Cell Research and Application, Kyoto University

Plenary Lecture 2 May 30 (Thu) 15:45 〜 16:45 Room 1 (ROHM Theatre Kyoto 1F South Hall)
Chairperson: Masahide Asano (Kyoto University)

PL-2

Regulation of phospholipid distribution in the lipid bilayer by ﬂippases and scramblases○ Shigekazu Nagata
Immunology Frontier Research Center, Osaka University

Special Lecture

11:10 〜 11:45

May 30 (Thu)

Room 4 (Miyako Messe B1F Main Conference Room )Special Lecture from KALAS (Korea)
SP-1

Chairperson: Masahide Asano (Kyoto University)

Molecular pathway of beneﬁcial effect of exercise in Germ Free mouse○ Je Kyung Seong
1)

1,2)
2)

College of Veterinary Medicine, Seoul National University, Korea Mouse Phenotyping Center (KMPC)

Open Public Lecture
May 31 (Fri)13:30 〜 15:30

Room 1 (ROHM Theatre Kyoto 1F South Hall)

Learning with Animals: From the Zoo to the African Jungle
Chairpersons: Masahide Asano (Kyoto University)
Chie Naruse (Kyoto University)

PLC-1 Activities of Kyoto City Zoo to support the well-being of wild and captive animals○ Yumi Yamanashi
Center for Research and Education of Wildlife, Kyoto City Zoo

PLC-2 A brief history of Japanese primatology and the last ape (Bonobo) society○ Gen’ ichi Idani
Wildlife Research Center, Kyoto University— 86 —

Symposium 1

May 29 (Wed)

9:30 ～ 11:50 Room 2 (ROHM Theatre Kyoto B2F North Hall)“Contributions to One Health ～ Current Status of Drug Resistances ～”Chairpersons: Kentaro Setoyama (Kagoshima University)
Hitoki Yamanaka (Shinshu University)

One Health has recognized internationally as a comprehensive approach to secure the health of peoples, animals,
and ecosystems. Recently, the spread of drug-resistant bacteria has been noticed in livestock and companion animals,
which are thought to affect human society, and the use of antibiotics must be carefully considered. In experimental
animals, the prevalence of drug-resistant bacteria may not be concerned due to improved microbiological control
techniques and minimized antibiotics usage. We therefore need to reconsider the prevalence of drug-resistant bacteria
in experimental animals and its impact. This symposium will provide the general and latest information on the drugresistances in humans, the environment, livestock, and companion animals, which would be important for us toreconsider the impacts of drug-resistances in experimental animals and learn the contributions to One Health.

S1-1Gain an overview of the circulation of AMR bacteria at the human-animal-environment interface○ Noriyuki Nagano
School of Health Sciences, Faculty of Medicine, Shinshu University

S1-2

Prevalence of antimicrobial resistant bacteria derived from livestock by perspective of One
Health○ Masaru Usui
Rakuno Gakuen University

S1-3

AMR in companion animals in Japan: current status and issues○ Kazuki Harada
Laboratory of Veterinary Internal Medicine, Tottori University

Symposium 2

May 29 (Wed)14:45 ～ 17:15 Room 1 (ROHM Theatre Kyoto 1F South Hall)“A New Stage in Memory Mechanism Research”

Chairpersons: Masahide Asano (Kyoto University)
Akihiro Goto (Kyoto University)

Memory mechanisms are one of the most important issues that humans have not been able to elucidate. Recent
genetic modification techniques using rodents, techniques for measuring neural activity and the use of various
memory tasks are beginning to reveal the reality of this problem. Dr. Goto will give a lecture on the development of
a new optogenetic method to erase synaptic LTP by light and the mechanism of long-term storage of memory. Dr.
Nomoto will give a lecture on the mechanisms by which multiple sensory information is integrated as memory, as
revealed by in vivo calcium imaging in mice. Dr. Fujisawa will give a lecture on the mechanism of spatial navigationthat predicts future movement by performing large-scale extracellular neural activity recordings in rats. Dr. Kida
will give a lecture on the mechanisms of fear memory remobilization and erasure using the fear conditioning context
task and the passive avoidance response task. The symposium will introduce latest studies that use cutting-edge
technology to uncover the mechanisms of memory.

S2-1

Elucidation of synaptic plasticity mechanism of long-term memory storage○ Akihiro Goto
Department of Pharmacology, Kyoto University Graduate School of Medicine— 87 —

S2-2

2+

Sensory information processing in the hippocampus through Ca imaging○ Masanori Nomoto
1,2,4)
Kaoru Inokuchi

1,2,3,4)

1,2)

1,2)

1,2)

, Emi Murayama , Shuntaro Ohno , Reiko Okubo-Suzuki ,1)Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama,
Research Center for Idling Brain Science, University of Toyama,
3)
4)Japan Agency for Medical Research and Development (AMED), CREST, JST
2)

S2-3

Neuronal representation for future spatial information in the hippocampus and entorhinal cortex○ Shigeyoshi Fujisawa
RIKEN Center for Brain Science

S2-4

Mechanisms of fear memory after retrieval○ Satoshi Kida
Graduate School of Agriculture and Life Sciences, The University of Tokyo

Symposium 3

May 29 (Wed)14:45 ～ 17:15Room 2 (ROHM Theatre Kyoto B2F North Hall)“Digital Transformation in Animal Testing Techniques Contributing to the
Advancement of the 3Rs”
Chairpersons: Mutsumi Suzuki (Non-Clinical Evaluation Expert Committee, Drug Evaluation Committee,
Japan Pharmaceutical Manufacturers Association (JPMA)/ Kyowa Kirin Co., Ltd.)
Kazuto Watanabe (Non-Clinical Evaluation Expert Committee, Drug Evaluation Committee,
Japan Pharmaceutical Manufacturers Association (JPMA))

Digital Transformation (Dx) has become indispensable, contributing to increased productivity, accuracy, andoptimization in a variety of areas. The utility of Dx is similar in animal studies. It improves the quality of animal
research by collecting and sharing accurate data free of human error, and improves efficiency by reducing work
time and the number of workers. It is also expected to contribute to the improvement of animal welfare by promptly
detecting and responding to animal abnormalities. This symposium will focus on the contribution of Dx to the 3Rs in
animal use, and will provide an opportunity to discuss the use of Dx from various perspectives in animal experiments.

S3-1

Implementation of Virtual Control Group in toxicology studies utilizing historical control data
○ Gen Sato

1,2)

1)

S3-2

2)

Global Nonclinical Regulatory Affairs, Eisai Co., Ltd., Japan Pharmaceutical Manufacturers Association

AI pathology in laboratory animal science and non-clinical research ﬁeld
○ Izumi Matsumoto

1,2)

1)

S3-3

2)

Sumitomo Pharma Co., Ltd., AI Pathology Task Force, Non-Clinical Evaluation Expert Committee, JPMA

Challenges to innovation in evaluation of clinical symptoms using novel wearable device
technology○ Ryota Tochinai
Vet. Pathophysiol. and Anim. Health, The University of Tokyo

S3-4

Contribution of e-learning video materials on proper animal experiments to the 3Rs○ Mariko Amano
KAC Co., Ltd.

— 88 —

S3-5

Vivarium management and 24/7 homecage monitoring with Digital transformation (DX)○ Yusuke Tanaka
Tecniplast Japan Co., Ltd.

Symposium 4

May 30 (Thu)

9:30 ～ 12:00

Room 1 (ROHM Theatre Kyoto 1F South Hall)“Cutting-Edge Cancer Research Utilizing Experimental Animals”
Chairpersons: Kazuhiro Okumura (Chiba Cancer Center Research Institute)
Chie Naruse (Kyoto University)

Using experimental animals in cancer research has provided significant insights over an extensive history. These
include a variety of models, such as tumor transplantation, carcinogenesis, genetically engineered animal models,and personalized medicine models, including immunotherapy. Various animal models have been developed, given
the inherent limitations of modeling cancer development within the human body. From genetic, pathological, andreproductive technology perspectives, experimental animals like rodents remain indispensable in cancer research.
In this symposium, five speakers will present the latest findings from various cancer research, ranging from basic
research to pre-clinical trials. We aim to provide an opportunity for active discussion and participation from a diverse
audience to deepen our understanding of cancer research, gain insights into new therapies and drug development, andrevisit the emerging role of experimental animal models in cancer biology.

S4-1

Analysis of senescent cells using a mouse model○ Akiko Takahashi

1,2)

1)
2)

S4-2

Division of Cellular Senescence, Cancer Institute, Japanese Foundation for Cancer Research,
Cancer Cell Communication Project, NEXT-Ganken Program, Japanese Foundation for Cancer Research

Cell competition between normal and transformed epithelial cells○ Yasuyuki Fujita
Kyoto University Graduate School of Medicine

S4-3

Creating a pancreatic cancer rat model using the pancreas-targeted hydrodynamic gene delivery○ Osamu Shibata, Kenya Kamimura, Yuto Tanaka, Hiroyuki Abe, Satoshi Ikarashi, Takeshi Yokoo,
Shuji Terai
Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences,
Niigata University

S4-4

Nonclinical evaluation of CAR-T cell products○ Yukari Fujiwara
Novartis Pharma K.K. Translational Medicine

S4-5

Approach for the prevention of hereditary cancer○ Shinya Toyokuni , Yingyi Kong , Yaguang Luo , Tatsuhiko Imaoka , Tomoji Mashimo
1)

1)

1)

1)

2)

3)

Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine,
Department of Radiation Effects Research, National Institute of Radiological Sciences,
National Institutes for Quantum Science and Technology,
3)
Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo2)— 89 —

Symposium 5

May 30 (Thu)

9:30 ～ 12:00 Room 2 (ROHM Theatre Kyoto B2F North Hall)“Infectious Diseases and Microbiological Control of Immunodeﬁcient Animals”
Chairpersons: Miyuu Tanaka (Osaka Metropolitan University)
Shinichiro Nakamura (Azabu University)

In recent years, severely immunodeficient animals such as NOG mice and genetically modified animals for
specific immune-related genes have been produced and used in various experiments. Immunocompromised animalmodels are susceptible to infection, and strict microbiological control is important in their breeding and management.
In this symposium, we will discuss the basics of immunodeficient animals (mice and rats), case studies of infectious
diseases, microbiological monitoring and husbandry in immunodeficient animals, and aim to deepen understanding ofappropriate microbiological quality control and husbandry of immunodeficient animals.

S5-1

History, development, and application of immune deﬁcient animals○ Takeshi Takahashi
Central Institute for Experimental Medicine and Life Science

S5-2

A case report of Pneumocystis outbreak in a breeding room of immunodeﬁcient mice
○ Shinichiro Nakamura , Ichiro Terakado , Hideaki Tsuchiya
1,2)

1)
2)

S5-3

2)

2)

Veterinary School of Medicine, Azabu University,
Research Center for Animal Life Sciences, Shiga University of Medical School

Rat polyomavirus 2 infection in a colony of immunodeﬁcient rats
○ Miyuu Tanaka

1,2)

1)

S5-4

2)

Veterinary Pathology, Osaka Metropolitan University, Institute of Laboratory Animals, Kyoto University

Murine Chapparvovirus (MuCPV) — a virus that causes renal disease in immunocompromised
mice○ Shigeri Maruyama
The Jackson Laboratory Japan, Inc.

S5-5

Microbiological quality control of immunodeﬁcient mice
○ Nobuhito Hayashimoto
ICLAS Monitoring Center, CIEM

S5-6

Breeding and management of severe immunodeﬁcient mice at CLEA Japan, Inc.
○ Megumi Nishiwaki
CLEA Japan, Inc.

— 90 —

Symposium 6

May 31 (Fri)9:30 ～ 12:00

Room 1 (ROHM Theatre Kyoto 1F South Hall)“Current Status of 3Rs in Animal Experimentation: International Guidelines,
Scientiﬁc Advancements, and Implementation Strategies”
Chairpersons: Shigiko Takei (Ina Research Inc.)
Hidenori Watanabe (JT Creative Service Inc.)

The symposium will focus on international guidelines related to the 3Rs and engage in a comprehensive
discussion about the progress of scientific and technological advancements alongside practical implementation
strategies. The purpose is to share the forthcoming developments toward the practical application of the 3Rs
principles (Reduction, Refinement, Replacement) based on the most recent international guidelines and to understandhow new guidelines and advancements in science and technology will be implemented in practice. Moreover, throughthis symposium, we aim to explore the direction of sustainable animal experimentation and research. Our goal is toprovide participants with an opportunity to consider new ideas and strategies for the future development of the 3Rs.

S6-1

Awareness and understanding of anesthesia for laboratory animal euthanasia in Japan andoverseas○ Atsushi Asano
Joint Faculty of Veterinary Medicine, Kagoshima University

S6-2

Risk assessment utilizing new approach methods promoted by the OECD
○ Yoko Hirabayashi
National Institute of Health Sciences

S6-3

Research trend and regulatory/industrial acceptance in microphysiological systems (MPS)○ Hitoshi Naraoka
Non-clinical Bioscience, Astellas Pharma Inc.

S6-4

The current state of the 3Rs in Pharmaceutical Toxicology Study Guidelines○ Mutsumi Suzuki
Non-Clinical Evaluation Expert Committee, Drug Evaluation Committee,
Japan Pharmaceutical Manufacturers Association

Symposium 7

May 31 (Fri)9:30 ～ 12:00

Room 2 (ROHM Theatre Kyoto B2F North Hall)“Laboratory Animal Bioresources for the New Era”
Chairpersons: Atsushi Yoshiki (RIKEN BRC)
Tomoji Mashimo (The University of Tokyo)

Experimental animals have become increasingly important as a fundamental resource for life science researches,
including toxicity testing, safety assessment, and as disease models for personalized medicine. On the other hand,genome editing technologies have been rapidly advancing, and the abundance of genomic and associated information
is becoming crucial for users. In this symposium, prominent representatives from national and internationalbioresource centers will explain in an accessible manner the changing landscape of these circumstances and the future
utilization of these resources for participants.

S7-1

Advancing mouse resource infrastructure program○ Atsushi Yoshiki
RIKEN BioResource Research Center— 91 —

S7-2

Past and future of the rat resource project○ Masahide Asano
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

S7-3

Why do we use monkeys for experiments?
○ Katsuki Nakamura
Center for the Evolutionary Origins of Human Behavior

S7-4

Chicken/ quail resources: new resources with genetic engineering○ Ken-ichi Nishijima, Yuya Okuzaki
Graduate School of Bioagricultural Sciences, Nagoya University

S7-5

Zebraﬁsh as a model experimental animal and NBRP
○ Hitoshi Okamoto
RIKEN Center for Brain Science

S7-6

Seoul National University Hospital Marmoset Model Network Center (SNUH-MMNC) and diseasemodel○ Byeong-Cheol Kang

1,2)

1)
2)

Seoul National University College of Medicine,
Biomedical Research Institute, Seoul National University Hospital

Symposium 8

May 31 (Fri)13:30 ～ 15:30 Room 2 (ROHM Theatre Kyoto B2F North Hall)“New Perspectives to Explore Animal Welfare”

Chairpersons: Naoko Hashimoto (Kyoto University)
Hironari Koyama (KAC Co., Ltd.)This symposium focuses on animal welfare in animal care and management, which is unfamiliar to animal
experimenters. Animal welfare is an important aspect to improve reproducibility of animal experiment results, and
it is necessary for technicians and researchers to have a better understanding of animal care and management. In
this symposium, three speakers will introduce animal welfare initiatives in various fields including laboratory small
animals, fishes, and farm animals. We would like to discuss with the speakers and audience participants how animal
welfare considerations for laboratory animals support animal experiments and how animal welfare can be promotedin the future. We hope that this symposium will provide an opportunity for both technicians and researchers to rethink
animal welfare, to gain knowledge and useful insights from discussions and exchanges of opinions among experts
involved in different fields, and to consider how to improve the accuracy of animal experiments.

S8-1

Anguish of laboratory animal Breeder — Balance between animal welfare and customer needs —○ Yohei Goto
The Jackson Laboratory Japan, Inc.

S8-2

Welfare of laboratory ﬁsh for animal drug development○ Kiyotaka Kawamura
Kyoritsu Seiyaku Corporation— 92 —

S8-3

Understanding and controlling of farm animal welfare○ Tsuyoshi Shimmura
Tokyo University of Agriculture and Technology

LAS Seminar 1

May 29 (Wed)9:00 ～ 11:30

Room 3（Miyako Messe B1F Special Exhibition Hall A）

I want to know! Experimental Animals part 3 (in Japanese)
Organizers: Hiroshi Kiyonari (RIKEN Center for Frontier Biosciences)
Ryuichi Miura (Life Science Research Ethics Support Ofﬁce, The University of Tokyo)

Seminar Outlines
In animal experimentation, it is crucial to select the appropriate species, maintain them in suitable environments
and handle them properly. Therefore, this seminar provides an opportunity to comprehend the characteristics of a
new laboratory animal species and acquire essential knowledge about its management, including acquisition, careand treatment as described below. We also encourage individuals already using the target animal species to take thisseminar as an opportunity to reconfirm their methods of care and handling. This year will mark the third installment
in a series that began two years ago and will cover bats , squirrel monkeys , naked mole rats and pigs .

- What advantages of use: biological classification, physiology/ecology, characteristics as a laboratory animal, statusof research use, etc.
- How to obtain: source, precautions for acquisition, relevant laws, etc.
- How to care: environmental condition, housing, feeding, environmental enrichment, etc.
- How to treat: picking up, holding, administration, etc.

Speakers:
LAS1-1

Shizuko Hiryu (Doshisha University)
No problem in the darkness! Bats manipulate their ability to‘see’through sound

LAS1-2Shin-ichi Yokota (The University of Tokyo)
Mischievous and amiable!“Saimiri boriviensis”

LAS1-3

Yoshimi Kawamura (Kumamoto University)
The secret of the naked mole-rat

LAS1-4

Arata Honda (Jichi Medical University)
Advancing medical research: the unique beneﬁts of using pigs as model organisms— 93 —LAS Seminar 2

May 30 (Thu)

9:00 ～ 11:30

Room 3（Miyako Messe B1F Special Exhibition Hall A）

Understanding animal feelings (behavior analysis)
Organizers: Kimie Niimi (Riken CBS)
Noboru Ogiso (NCGG)

Seminar Outlines:
This seminar will present examples of behavior analysis using frogs, bats, pigeons, mice, common marmosets,
and macaque monkeys, as well as the analysis of the results. The lectures will cover a wide range of topics from an
overview of animal behavior science to the latest research results on efforts to understand animal feelings fromanimal behavior.

Speakers:
LAS2-1

Nozomi Nishiumi (NBB)
Adaptive decision-making explored through the tactics of predators and prey

LAS2-2

Kazunari Miyamichi (RIKEN BDR)
Functional dissection of hypothalamus underlying emotion and instinct behaviors:
lessons from aggressive and parental behaviors in mice

LAS2-3Teppei Ebina (The University of Tokyo)
Development of behavior tasks for functional imaging of neuronal activity in common
marmosets

LAS2-4

Kentaro Miyamoto (RIKEN CBS)
Investigation of introspective ability of macaque monkeys during foraging tasks andnatural behaviour observation— 94 —LAS Seminar 3

May 31 (Fri)9:00 ～ 11:30

Room 3（Miyako Messe B1F Special Exhibition Hall A）

Getting started with bioinformatics
Organizers: Ayako Isotani (Nara Institute of Science and Technology)
Takashi Inoue (Central Institute for Experimental Medicine and Life Science)

Seminar Outlines:
Understanding biological phenomena requires analysis of various components such as RNA transcribed fromgenomic DNA, proteins which are the final products of genes, and various metabolic products synthesized in the bodythrough protein functions. In recent years, life science research has made it possible to easily obtain a vast amountof information with the advancement of high-throughput instruments, as typified by next-generation sequencers. On
the other hand, bioinformatics is essential to narrow down the information from these vast datasets and understandbiological phenomena.
Among wet-lab researchers and technicians, who mainly focus on animal experiments, some may have aslight discomfort with bioinformatics. However, recent findings reported in top-tier life science journals using
bioinformatics underscore its crucial role in understanding new biological phenomena.
In this seminar, experts in single-cell RNA sequencing analysis, proteomic analysis, metabolomic analysis,
and transomic analysis will deliver lectures on the characteristics and applications of each field for beginners inbioinformatics. We hope that beginners in bioinformatics will utilize this knowledge to advance their researchendeavors.

LAS3-1

Itoshi Nikaido (RIKEN BDR)
Guided by AI: practical analysis of single-cell RNA sequencing data

LAS3-2

Yusuke Kawashima (Kazusa DNA Research Institute)
Introduction to proteomics and its data analysis

LAS3-3

Yuki Sugiura (Kyoto University)
Spatial metabolomics -exploring metabolic biochemistry with molecular imaging

LAS3-4

Toshiya Kokaji (Nara Institute of Science and Technology)
Pathway estimation from omic datasets for wet laboratory

Best Presentation Award

May 29 (Wed) 9:30 ～ 12:00

Room 1 (ROHM Theatre Kyoto 1F South Hall)

May 30 (Thu) 17:00 ～ 18:00

Poster & Exhibiton (Miyako Messe 1F Exhibition Hall C,D)

Poster presentaion

Chairpersons: Seiji Kito (Nagoya University)
Natsuki Matsushita (Aichi Medical University)

BP-01 Study on proteins and glycans that play an important role in homing of hematopoietic stem cells○ Xuchi Pan, Chie Naruse, Tomoko Matsuzaki, Kazushi Sugihara, Masahide Asano
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University— 95 —

BP-02 Investigation of functional exons in organogenesis using a novel genome editing mouse series○ Hayate Suzuki, Akihiro Kuno, Kazuya Murata, Fumihiro Sugiyama, Seiya Mizuno
Laboratory Animal Resource Center, Institute of Medicine, University of Tsukuba

BP-03 Seminoripid is required for Sertoli cell polarity and the function of blood-testis barrier○ Kanon Akiyama , Kousuke Noritake , Airi Nakano , Mikio Furuse , Tatsuyuki Yamashita ,
5)
6)
6,7)
8)
9,10)
Kouishi Honke , Manabu Ozawa , Masahito Ikawa , Yoshitaka Fujihara , Tokuko Iwamori ,
11)
12)
12)
1,13,14)
Seiya Mizuno , Narumi Ogonuki , Atsuo Ogura , Seiji Takashima
1)

1)

1)

2)

3)

1)

2,3)

4)

4)

5)

Shinshu Univ., NIPS, SOKENDAI, Kochi University Medical School, Kochi Univ.,
7)
8)
9)
10)
11)
The Univ. of Tokyo, Osaka Univ., NCCC, Kyushu Univ., JSPS-RPD, Univ. of Tsukuba,
12)
13)
14)
RIKEN BRC, ICCER, Shinshu Univ., SSXI, Shinshu Univ.
6)

BP-04 Development of AAV vector-mediated knock-in in porcine embryos○ Mitsuhiro Noguchi , Fuminori Tanihara , Yuka Inage , Makoto Inoue , Yutaka Hanazono ,2)
1)
Takashi Yokoo , Arata Honda
1)

1)

1)

2)

1,3)

2)

1)

3)

Jichi Medical University, The Jikei University School of Medicine, Sumitomo Phama Co., Ltd.

BP-05 A newly developed Kit mutat rat allows for hematopoietic stem cell engraftment without
irradiation○ Ryuya Iida , Saeko Ishida , Jinxi Wang , Kosuke Hattori , Kazuto Yoshimi , Satoshi Yamazaki ,1,2)
Tomoji Mashimo
1)

1)

1)

1)

1,2)

3,4)

1)

Division of Animal Genetics, Laboratory Animal Research Center, IMSUT, The University of Tokyo,
Division of Genome Engineering, Center for Experimental Medicine and Systems Biology, IMSUT,
The University of Tokyo,
3)
Division of Cell Regulation, Center of Experimental Medicine and Systems Biology, IMSUT,
The University of Tokyo,
4)
Laboratory of Stem Cell Therapy, Faculty of Medicine, University of Tsukuba
2)

BP-06 Skin graft with dermis and appendages generated in vivo by cell competition○ Naoaki Mizuno , Hisato Nagano , Hideyuki Sato , Eiji Mizutani , Ayaka Yanagida ,
2,6)
2)
2)
2)
7)
Mayuko Kano , Mariko Kasai , Hiromi Yamamoto , Motoo Watanabe , Fabian Suchy ,
2)
1)
2,7)
Hideki Masaki , Masami Kanai , Hiromitsu Nakauchi
1,2)

2,3)

2)

2,4)

2,5)

1)

Center for Experimental Animals, Tokyo Medical and Dental University,
Stem Cell Therapy Laboratory, Tokyo Medical and Dental University,
3)
Department of Plastic and Reconstructive Surgery, National Defense Medical College,
4)
Laboratory of Stem Cell Therapy, University of Tsukuba,
5)
Department of Veterinary Anatomy, The University of Tokyo,
6)
Metabolism and Endocrinology, St. Marianna University School of Medicine,
7)
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
2)

BP-07 Exocyst complex is essential for oogenesis in mice
○ Kim Chi Lieu Nguyen , Woojin Kang , Fumihiro Sugiyama , Atsuo Ogura , Satoru Takahashi ,2)
Seiya Mizuno
1)

2)

2)

3)

2)

1)

PhD. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba,
Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba,
3)
Bioresource Engineering Division, RIKEN BioResource Research Center2)

BP-08 Selective breeding and gut microbiota: role of intestinal bacteria in domestication of mice
○ Bhim Bahadur Biswa , Hiroshi Mori , Atsushi Toyoda , Ken Kurokawa , Tsuyoshi Koide
1,2)

2,3)

4)

1)

2,5)

Mouse Genomics Resource Lab, National Institute of Genetics,
3)
Graduate University for Advanced Studies (SOKEDNAI), Genome Diversity Lab, NIG,
4)
5)
Comparative Genomics Lab, NIG, Genome Evolution Lab, NIG
2)— 96 —

1,2)

BP-09 Pate family genes expressed in the epididymis cooperatively support sperm maturation○ Hina Shinohara , Ayumu Taira , Kimi Araki , Masahito Ikawa , Taichi Noda
1)

1)

1)

1)

2)

1,2)

2)

IRDA, Kumamoto University, RIMD, Osaka University

BP-10 Effects of parent-child separation during infancy on brain gene expression in marmosets○ Haruka Shinohara , Makiko Meguro-Horike , Takashi Inoue , Miyuki Shimazu ,
4)
4)
4)
1)
2,3)
Machiko Hattori , Hitoshi Hibino , Kazumasa Fukasawa , Erika Sasaki , Shin-ichi Horike
1,3)

2)

1)

2)

1)

Central Institute for Experimental Medicine and Life Science,
Research Center for Experimental Modeling of Human Disease, Division of Integrated Omics Research,
Kanazawa University,
3)
United Graduate School of Child Development, Osaka University, Kanazawa University,
Hamamatsu University School of Medicine, Chiba University and University of Fukui,
4)
Yaotsu Breeding Center, CLEA Japan, Inc.
2)

BP-11 Development of human IgH/Ig λ antibody-producing mice by using chromosome engineering
technology○ Kazuto Shimoya , Shigenori Baba , Takashi Moriwaki , Satoshi Abe , Akane Okada ,3)
1,2,3,4)
Kanako Kazuki , Yasuhiro Kazuki
1)

2)

1,2)

3)

3)

1)

Department of Chromosome Biomedical Engineering, Graduate School of Medical Sciences,
Tottori University,2)
Department of Chromosome Biomedical Engineering, School of Life Science, Faculty of Medicine,
Tottori University,
3)
Chromosome Engineering Research Center (CERC), Tottori University,
4)
Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems(ExCELLS), National Institutes of Natural Sciences

BP-12 Establishment of a humanized mouse model for contact hypersensitivity induced by humanTh2 cells○ Yusuke Ohno , Misa Mochizuki , Kenji Kawai , Yukio Nakamura , Ryuji Suzuki ,
1)
1)
1)
1)
Motohito Goto , Riichi Takahashi , Mamoru Ito , Ryoji Ito
1)

1)

1)

1)

2)

2)

2)

Central Institute for Experimental Medicine and Life Science, Repertoire Genesis Inc.

Oral Presentation

Oral Presentation 1 (O-01 〜 17)
May 29 (Wed) 9:00 〜 11:50 Room 4 (Miyako Messe B1F Main Conference Room)
Oral Presentation 2 (O-18 〜 33)
May 29 (Wed) 14:40 〜 17:20 Room 3 (Miyako Messe B1F Special Exhibition Hall A)
Oral Presentation 3 (O-34 〜 43)
May 29 (Wed) 14:40 〜 16:20 Room 4 (Miyako Messe B1F Main Conference Room)
Oral Presentation 4 (O-49 〜 59)
May 30 (Thu) 9:00 〜 10:50 Room 4 (Miyako Messe B1F Main Conference Room)
Oral Presentation 5 (O-60 〜 74)
May 31 (Fri) 9:00 〜 11:30 Room 4 (Miyako Messe B1F Main Conference Room)— 97 —

Poster Presentation

May 29 (Wed) 9:00 〜 30 (Thu) 18:00
Room: Poster & Exhibition (Miyako Messe 1F Exhibition Hall C,D)Core Time:May 29 (Wed) 17:30 〜 18:30 (Odd numbers of P-001 〜 137)
May 30 (Thu) 17:00 〜 18:00 (Even numbers of P-001 〜 137 and BP-01 〜 12)
Luncheon Seminar LS-1 (Sponsor Name: CLEA Japan Inc., Tokyo, Japan)

May 29 (Wed) 12:15 〜 13:15 Room 2 (ROHM Theatre Kyoto B2F North Hall)
Chairperson: Masami Shinohara (CLEA Japan Inc., Tokyo, Japan)
Title: Unmet medical needs and repositioning of animal models
Speaker: Takeshi Ohta (Laboratory of Animal Physiology and Functional Anatomy, Graduate School of
Agriculture, Kyoto University, Kyoto, Japan)
Luncheon Seminar LS-2 (Sponsor Name: The Jackson Laboratory Japan, Inc.)

May 29 (Wed) 12:15 〜 13:15 Room 3 (Miyako Messe B1F Special Exhibition Hall A)
Chairperson: Takashi Ueda (The Jackson Laboratory Japan, Inc.)
Title: JAX® Mice: Unique Models for Ground-Breaking Biomedical Discoveries
Speaker: Aya Uchida (The Jackson Laboratory Japan, Inc. Technical Information Services)
Luncheon Seminar LS-3 (Sponsor Name: Natsume Seisakusho Co., Ltd.)May 29 (wed) 12:15 〜 13:15 Room 4 (Miyako Messe B1F Large Conference Room)
Chairperson: Teppei Ogawa (Natsume Seisakusho Co., Ltd.)
Title: Animal Experiment Equipment Moves to the Next Stage with the AUTiv Mouse Tail
Vein Auto Injection System Using Deep Learning
Speaker: Takuya Inoue (Preferred Networks, Inc.)
Takashi Koshikawa (Summit Pharmaceuticals International Corporation)
Teppei Ogawa (Natsume Seisakusho Co., Ltd.)
Luncheon Seminar LS-4 (Sponsor Name: MIURA CO.,LTD.)May 30 (Thu) 12:15 〜 13:15 Room 2 (ROHM Theatre Kyoto B2F North Hall)
Chairperson: Erika Sasaki (Central Institute for Experimental Medicine and Life Science)
Title: Innovative Approaches to Energy Conservation in Cleaning & Sterilization Practices
Speaker: Naoto Abe (MIURA CO.,LTD.)
Luncheon Seminar LS-5 (Sponsor Name: KAC CO.,Ltd.)May 30 (Thu) 12:15 〜 13:15 Room 3 (Miyako Messe B1F, Special Exhibition Hall A)
Chairperson: Hironari Koyama (KAC CO.,Ltd.)
Title 1: Genotyping of G0 generation genome-edited mice
Speaker: Seiya Mizuno (University of Tsukuba, Institute of Medicine, Transborder Medical
Research Center, Laboratory Animal Resource Center)
Title 2: How do we deal with such a situation? FAQ on primate husbandry
Speaker: Naoko Hashimoto (Center for the Evolutionary Origins of Human Behavior, Kyoto University)
Luncheon Seminar LS-6 (Sponsor Name: The Jackson Laboratory Japan, Inc.)

May 30 (Thu) 12:15 〜 13:15 Room 4 (Miyako Messe B1F Large Conference Room)
Chairperson: Shigeri Maruyama (The Jackson Laboratory Japan, Inc.)
Title: The usefulness of environmental samples in terms of the detection status of pathogenic
microorganisms and the stability of bacteria in the environment
Speaker: Yuki Suzuki (The Jackson Laboratory Japan, Inc. Monitoring center)— 98 —

Luncheon Seminar LS-7 (Sponsor Name: Avidity Science Co., LTD.)May 31 (Fri) 12:15 〜 13:15 Room 3 (Miyako Messe B1F, Special Exhibition Hall A)
Chairperson: Ryoichi Sugimoto (Avidity Science Co., LTD.)
Title: Animal Welfare, your drinking systems options
Speaker: Mike Douglas (Allentown, LLC)
Equipment Exhibition

May 29 (Wed) 9:00 〜 31 (Fri) 12:00
Room: Poster & Exhibition (Miyako Messe 1F Exhibition Hall C,D)
Hospitality RoomMay 29 (Wed) 9:00 〜 31 (Fri) 12:00
Venue: Miyako Messe B1F【HR-1】 CLEA Japan (Craft Room)【HR-2】 Central Institute for Experimental Medicine and Life Science (CIEM) (Conference Room 1)【HR-3】 ARK Resource (Conference Room 2)【HR-4】 The Jackson Laboratory Japan (Conference Room 3)
Social Gathering (Banquet)

May 30 (Thu) 18:30 〜 20:30
Hotel Okura Kyoto, 4F Gyoun（暁雲）— 99 —