⬨᳝ື≀ࡢᅄ⫥෌⏕⬟ຊ࡟✀ᕪࢆࡶࡓࡽࡍ࣓࢝ࢽࢬ࣒ࡢ◊✲Analysis of molecular mechanisms underlying the species differencesin Verteblate limb regeneration㛗὾ࣂ࢖࢜኱Ꮫ   ᕝཱྀ ⲁὴ㐵ᮇ㛫    2015 ᖺ 11 ᭶ 1 ᪥㹼2016 ᖺ 4 ᭶ 30 ᪥◊✲ᶵ㛵 
CRTD / DFG-Forschungszentrum für Regenerative Therapien Dresden◊✲ᣦᑟ⪅  Professor Dr. Elly Margaret Tanaka

Higher vertebrate cannot regenerate when they lost organs like limbs due to limitation of regeneration capacity. On
the other hand, amphibians such as newt and axolotl can regenerate their lost organs with perfect shape and function.
However, it is unclear the mechanisms defining regeneration capacity of amphibians. I hypothesized that the differencesof regeneration ability between animal species are based upon the differences of epi-genome transcriptional regulation
mechanisms. In order to analyze epi-genome regulation in amphibian, perfect genome information of this species is needed.
The laboratory where I stay in this study abroad, is the only one having axolotl (one of amphibians) genome information.
However, the whole consecutive genome sequences had not been assembled yet. Thus, I first tried to assemble multiplegenome fragments to complete whole axolotl genome sequence. Actually, I had contributed to establish High-through put
Chromatin Conformation Capture method (HiC) to assemble the genome pieces as one of genome project members. Now
I had established the method and I had tried to assemble the genome using the HiC results. Finally, the axolotl genome isalmost assembled and there are just 65 scaffolds. This success of the axolotl genome project would be great help for thefuture regeneration research.
◊✲┠ⓗ  ᚚࡢ㐪࠸ࡣ࡝ࡢࡼ࠺࡞ศᏊᇶ┙ࡢ㐪࠸࡟ࡼࡗ࡚⏕ࡌ ⬨᳝ື≀ࡢ෌⏕⬟ຊࡣ✀㛫࡛኱ࡁࡃ␗࡞ࡿࠋ౛࠼࡚࠸ࡿࡢ࠿ࠊᮍࡔ᫂ࡽ࠿࡛ࡣ࡞࠸ࠋࡇࡢ෌⏕⬟ຊࡢࡤࠊ့ங㢮ࡣኻࡗࡓᡭ㊊ࢆ෌⏕࡛ࡁ࡞࠸ࡀࠊ࢖ࣔࣜ✀ᕪࢆ⏕ࡳฟࡍ௙⤌ࡳࢆゎ᫂ࡍࡿࡇ࡜ࡣࠊࣄࢺࡢ㞃ࡸ࢔࣮࣍ࣟࢺࣝ࡜࠸ࡗࡓ᭷ᑿ୧⏕㢮ࡣ࡜ࡾࢃࡅ෌⏕ࡉࢀࡓ෌⏕⬟ຊࢆ᫂ࡽ࠿࡟ࡍࡿ✺◚ཱྀ࡜࡞ࡿㄢ㢟࡛⬟ຊࡀ㧗ࡃࠊኻࡗࡓᡭ㊊ࡢࡳ࡞ࡽࡎࠊᚰ⮚ࡍࡽ෌⏕࠶ࡿࠋࡑࡇ࡛⏦ㄳ⪅ࡣࠊ࢔࣮࣍ࣟࢺࣝࡢᅄ⫥෌⏕࡟ࡍࡿࡇ࡜ࡀ࡛ࡁࡿࠋ୍᪉࡛ࠊྠࡌ୧⏕㢮࡛ࡶࢶ࣓࢞࠾ࡅࡿ఩⨨᝟ሗࢆྖࡿ㑇ఏᏊ⩌ࡢࢡ࣐ࣟࢳ࢚ࣥࣞ࣋ࣝࣝࡢᡂయࡣࠊኻࡗࡓᡭ㊊ࢆ㒊ศⓗ࡟ࡋ࠿෌⏕ࡁࡎ

࡛ࡢⓎ⌧ไᚚ࡟ὀ┠ࡋࠊ᏶඲࡞୕ḟඖჾᐁࢆྍ⬟࡟ࠊࢫࣃ࢖ࢡ࡜ࡼࡤࢀࡿᴟᛶࡢ࡞࠸ᵓ㐀ࢆ෌⏕ࡍࡿࡍࡿ࣓࢝ࢽࢬ࣒ࢆ᫂ࡽ࠿࡟ࡍ࡭ࡃࠊὴ㐵ඛ◊✲ᐊ࡟㸦Endo H., et al., 2000㸧ࠋࡇࡢ✀࡟ࡼࡿ෌⏕⌧㇟ࡢ࠾࠸࡚ゎᯒࢆ㛤ጞࡋࡓࠋ㐪࠸ࡣࠊ୍ࡘࡣ㑇ఏᏊࡢⓎ⌧ไᚚᶵᵓࡢ㐪࠸࡟౫Ꮡ ὴ㐵ᙜึࠊࣔࢹࣝ࡜ࡍࡿ࢔࣮࣍ࣟࢺࣝࡢࢤࣀ࣒᝟ࡍࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿࠋ඲ᣦᥞࡗࡓᡭ㊊ࢆ෌⏕ࡍࡿሗࡣᮍࡔ᫂ࡽ࠿࡛࡞ࡃࠊὴ㐵ඛ◊✲ᐊ࡛ୡ⏺࡟ඛ㥑࢔࣮࣍ࣟࢺ࡛ࣝࡣࠊ෌⏕⫥ⱆ࡟࠾࠸࡚ᡭࡢࡦࡽࡢࣃࡅ࡚ゎㄞࡀヨࡳࡽࢀ࡚࠸ࡿ᭱୰࡛࠶ࡗࡓࠋࡑࡢࡓࡵ

ࢱ࣮ࣥᙧᡂࢆᨭ㓄ࡍࡿ Sonic hedgehog (Shh)㑇ఏᏊࡀࠊ⏦ㄳ⪅ࡣᮏㄢ㢟ࢆ㐙⾜ࡍࡿࡓࡵ࡟ࠊ࢔࣮࣍ࣟࢺࣝ㐺ษ࡞⟠ᡤ࡟Ⓨ⌧ࡍࡿࠋࡋ࠿ࡋ࡞ࡀࡽࠊ㸯ᮏᣦࡋ࠿ࡢࢤࣀ࣒ࣉࣟࢪ࢙ࢡࢺ࡟ཧຍࡋࠊ㈈ᅋ࡟ࡼࡿᨭ᥼ࢆ

෌⏕ࡋ࡞࠸ࢶ࣓࢚࢞ࣝᡂయ࡛ࡣ Shh ࡀⓎ⌧ࡏࡎ㸦ኚ࠸ࡓࡔ࠸࡚࠸ࡿᮇ㛫࡛ࡣࠊ◊✲ᇶ┙ࡢ❧ࡕୖࡆࢆ⾜ែᚋࠊⓎ⌧ࡋ࡞ࡃ࡞ࡿ㸧ࠊࡇࡢࢶ࣓࢚࢞ࣝࡢ୍ᮏᣦ࠺ࡇ࡜ࢆ┠ⓗ࡟◊✲ࢆ⾜ࡗࡓࠋ෌⏕⫥ⱆ࡟ Shh ⺮ⓑ㉁ࢆ௜୚ࡍࡿ࡜」ᩘࡢᣦࡀᙧᡂ
ࡉࢀࡿࡇ࡜ࡀ᫂ࡽ࠿࡟࡞ࡗ࡚࠸ࡿ㸦Yakushiji N., et◊✲⤒㐣࡜⪃ᐹ

al., 2009㸧ࠋࡲࡓ⭎ࡢᇶ㒊ඛ➃ࢆྖࡿ㑇ఏᏊ⩌࡛࠶ࡲࡎࠊὴ㐵᫬Ⅼ࡛ࡣࠊ࢔࣮࣍ࣟࢺࣝࡢࢤࣀ࣒᝟ሗࡣ

ࡿ HoxA/D 㑇ఏᏊ⩌ࡢⓎ⌧ࡀ஘ࢀࡿࡇ࡜ࡀሗࡉࢀ࡚

ᖹᆒ 25000bp ⣙ 200,000 ᩿∦࠿ࡽ࡞ࡿ PACBIO࠸ࡿ㸦Ohogo Y., et al., 2011㸧ࠋ ࡇࡢ㑇ఏᏊⓎ⌧ไ㸦Pacific Bioscience sequencing㸧࡟ࡼࡿࢤࣀ࣒᝟ሗ࡟㝈ࡽࢀ࡚࠸ࡓࠋ࢔࣮࣍ࣟࢺࣝࡢࢤࣀ࣒㛗ࡣࠊ࣐࢘ࡋࡓࢧࣥࣉࣝ࡜ࡣ␗࡞ࡿࢡ࣐ࣟࢳࣥ≧ែࢆ♧ࡍྍ⬟ࢫࡸ့ங㢮ࡢࢤࣀ࣒㛗ࡢ 10 ಸ࡜ண ࡉࢀ࡚࠾ࡾ㸦Uᛶࡀ࠶ࡿࠊ㸦㸰㸧ᇶ♏◊✲࡞࡝࡟ࡼࡃ⏝࠸ࡽࢀࡿ့

npublished data㸧ࠊ◊✲ࣔࢹࣝ࡜ࡋ࡚฼⏝ྍ⬟࡞≧ែங㢮ࡢᇵ㣴⣽⬊㸦NIH3T3 ⣽⬊ᰴࡸ Cos7 ⣽⬊ᰴ➼㸧࡟ࡍࡿࡓࡵ࡟ࡣࠊࡇࡢ᩿∦㞟ࢆṇࡋࡃ⧅ࡆࡿᚲせࡀ࠶ࡿࠋࡑࡢࡓࡵ࡟ࠊ⏦ㄳ⪅ࡣ de novo ࢤࣀ࣒࢔ࢵࢭࣥࣈࣜࢆ┠ⓗ࡜ࡋࡓ HiC/High-throughput chromatin

࡜ẚ㍑ࡍࡿ࡜ AL1 ⣽⬊ ࡣ⣽⬊࿘ᮇࡀ㐜࠸ࠊ㸦㸱㸧
AL1 ⣽⬊ࡢ┤ᚄࡣࠊࡇࢀࡽࡢ⣽⬊࡜ẚ࡭࡚⣙ 3-4 ಸ

conomation capture ࡢ❧ࡕୖࡆࢆ⾜࡞ࡗࡓࠋᐇ㦂㛤ጞ࠶ࡾࠊࣇࣛࢫࢥ༢఩ᙜࡓࡾ࡛ᅇ཰࡛ࡁࡿ⣽⬊ᩘࡀ⣙ᙜึࠊ࢔࣮࣍ࣟࢺࣝࡢ⫢⮚⣽⬊ࢆ⏝࠸ࡓ HiC ἲࡢ☜

1/10 ⛬ᗘ࡛࠶ࡿࡇ࡜ࠊ࡞࡝ࡢⅬࡀ࠶ࡆࡽࢀࡿࠋ㸦㸯㸧❧ࢆヨࡳࡓࠋ ⫢⮚ࡣ⣽⬊ᩘࡀከࡃࠊ኱ࡁ࡞⮚ჾ࡛࡟ࡘ࠸࡚࡛࠶ࡿࡀࠊAL1 ⣽⬊ࡣ࢟ࣕࣛࢡࢱࣜࢮ࣮ࢩ࠶ࡿࡓࡵࠊ୍ಶయ࠿ࡽࡢ⣽⬊ᩘࡀ☜ಖ࡛ࡁࡿ࡜⪃࠼ࣙࣥࡀ⾜ࢃࢀ࡚࠸࡞࠸⣽⬊ᰴ࡛࠶ࡿࠋࡑࡢࡓࡵࠊࢡࡓ㸦1x10^7 ⣽⬊ࡀᚲせ࡛࠶ࡿ㸧ࠋࡋ࠿ࡋ࡞ࡀࡽࠊᐇ࣐ࣟࢳࣥࡢゎᯒࣔࢹࣝ࡜ࡋ࡚⏝࠸ࡿ࡟ࡣ୙☜ᐃせ⣲㦂ࢆ㐍ࡵࡿ࠺ࡕ࡟ࠊ㸦㸯㸧⫢⮚ࡀ⬡㉁ࢆከࡃྵࡴࢧ
ࣥࣉ࡛ࣝ࠶ࡿࡇ࡜ࠊࡲࡓ⫢⮚࡟Ꮡᅾࡍࡿ⬡㉁ࢆ㝖ࡃࢫࢸࢵࣉࡣࢡ࣐ࣟࢳࣥࡢ┦஫స⏝ࢆኚ໬ࡉࡏ࡚ࡋࡲࡀከ࠸ࠋ㸦2㸧࡜㸦3㸧ࡣࠊHiC ἲ࡟ᚲせ࡞ 1x10^7 ࡢ⣽⬊ࢆᅇ཰ࡍࡿࡇ࡜ࢆᅔ㞴࡟ࡍࡿせᅉ࡛࠶ࡿࠋࡑࡢ࠺ࡇ࡜ࠊ㸦㸰㸧⫢⮚࡟ྵࡲࢀࡿ㉥⾑⌫ࡀ㠀≉␗ⓗ࡞⣽⬊࿘ᮇࡢ㐜ࡉࡺ࠼ࠊࢥࣥࣇ࢚ࣝࣥࢺ࡟฿ࡿࡲ࡛࡟ゎᯒࢩࢢࢼࣝࢆ⏕ࡳฟࡍࡇ࡜ࠊࡀࢃ࠿ࡗࡓࠋࡲࡎࠊ

2-3 㐌㛫࠿࠿ࡿୖࠊ1 ࣇࣛࢫࢥ࠶ࡓࡾ࡟ᅇ཰࡛ࡁࡿᩘ㸦㸯㸧ࡢၥ㢟ࢆゎỴࡍࡿࡓࡵ࡟ࠊ⬡㉁ࢆྲྀࡾ㝖ࡃ᪉

ࡀ 0.3x10^6 ⣽⬊࡛࠶ࡿࡇ࡜࠿ࡽࠊᐇ㉁ 30 ᯛ⛬ᗘࡢἲࢆྲྀࡾධࢀࡓࡀࠊ᰾ᰁⰍࡢ⤖ᯝ࠿ࡽࠊ᰾ࡀ⫧኱ࡋࣇࣛࢫࢥࡀᚲせ࡜࡞ࡿࠋ୍ᗘࡢヨ⾜ᐇ㦂࡛ 2-3 㐌㛫ࡓࡼ࠺࡞ᙧ࡟ኚ໬ࡋ࡚࠸ࡿࡇ࡜ࡀࢃ࠿ࡗࡓࠋࡑࡢࡇ

࠿࠿ࡾࠊࡲࡓࡇࡢࣇࣛࢫࢥࡢᩘᏐࡣ⌧ᐇⓗ࡞ᩘ࡛ࡣ࡜࠿ࡽࠊࢡ࣐ࣟࢳࣥࡢᮏ᮶ࡢ┦஫స⏝ࢆኚ໬ࡉࡏ࡚࠸ࡿྍ⬟ᛶࡀ㧗ࡃࠊࢧࣥࣉࣝ࠿ࡽ⬡㉁ࢆ㝖ࡃࡇ࡜ࡣ࡛ࡁ࡞࠸࡜ุ᩿ࡋࡓࠋ㸦㸰㸧࡟ࡘ࠸࡚࡛࠶ࡿࡀࠊ୧

࡞࠿ࡗࡓࡓࡵࠊ᭱ึࡢヨ⾜࡛ࡣ࣐ࢸࣜ࢔ࣝ࡜ࡋ࡚୙㐺ษ࡜ࡋุ࡚᩿ࡋ࡚࠸ࡓࠋࡋ࠿ࡋࠊࡑࡢ୍᪉࡛ࠊࡇ⏕㢮ࡢ㉥⾑⌫ࡣᡃࠎ࡜␗࡞ࡾ᭷᰾࡛ࠊ࣒࣍ࣝ࢔ࣝࢹࡢ⣽⬊ࢆ౑࠺᭱኱ࡢ฼Ⅼࡣࠊ⣽⬊㞟ᅋࡀ࠶ࡿ⛬ᗘᆒࣄࢻᅛᐃ࡟ࡼࡿ⣽⬊ᅇ཰ࡢ㝿࡟ࠊ㉥⾑⌫ࡶྠ᫬࡟ᅛ୍࡞࣏ࣆ࣮ࣗࣞࢩ࡛ࣙࣥ࠶ࡿ࡜ண᝿ࡉࢀࡿࡇ࡜࡛࠶ᐃࡉࢀࡿࠋࡇࡢ᫬ࠊ㉥⾑⌫ࡀከࡃྵࢇ࡛࠸ࡿ≉ᚩⓗࡿࠋࡘࡲࡾࠊAL1 ⣽⬊ࡣୖ⭎ࡢ෌⏕㒊఩࠿ࡽᶞ❧ࡉ࡞⣽⬊㦵᱁㸦ࢫ࣌ࢡࢺࣜࣥ㸧ࡣ࣒࣍ࣝ࢔ࣝࢹࣄࢻ࡟ࢀࡓ⣽⬊ᰴ࡛࠶ࡿࡓࡵ➽ᖿ⣽⬊ࡸ㦵ⱆ⣽⬊࡞࡝ከࡃࡼࡗ࡚ᙉࡃᯫᶫࡉࢀࡿࡇ࡜ࡀࢃ࠿ࡗࡓࠋHiC ゎᯒ࡛ࡢ⣽⬊✀ࢆྵࡴ࡜ண᝿ࡉࢀࡿࡀࠊᶞ❧ᚋ㛗ࡽࡃ⥅௦⏝࠸ࡿ᰾ࢧࣥࣉࣝࡣࠊ࣒࣍ࣝ࢔ࣝࢹࣄࢻ࡛ᆒ୍࡟ె
ቃࡉࢀࡿᚲせࡀ࠶ࡿࠋࡋ࠿ࡋ࡞ࡀࡽ࠸ࡃࡘ࠿ࡢ࣒࣍ࣝ࢔ࣝࢹࣄࢻᯫᶫ࡟ࡘ࠸࡚ࡢ᮲௳᳨ウ࠿ࡽࠊ⫢⮚ࢆࡉࢀ࡚ࡁࡓࡓࡵࠊඃ఩࡟ศ⿣⬟ຊࢆᣢࡗࡓ⤖ྜ⤌⧊⏤᮶ࡢ⣽⬊࡟㑅ᢥࡉࢀ࡚࠸ࡿ࡜⪃࠼ࡓࠋ⫢⮚ࢆ࣐ࢸᵓᡂࡍࡿ⣽⬊ࡢ୍ࡘ࡛࠶ࡿ࣊ࣃࢺࢧ࢖ࢺ࡞࡝ࡢ⣽⬊ࣜ࢔ࣝ࡜ࡋࡓ㝿࡟⏕ࡌࡓࠊ࣊ࣃࢺࢧ࢖ࢺࠊ⬡㉁ࠊ㉥࡜㉥⾑⌫⣽⬊ࡣ࣒࣍ࣝ࢔ࣝࢹࣄࢻ࡟ᑐࡍࡿ⭷ࡢᾐ㏱⾑⌫࡞࡝ࡢከࡃࡢ⣽⬊✀ࡀዃ㞧ࡍࡿྍ⬟ᛶࡣప࠸ࠋ

ᛶ࡜ࢱࣥࣃࢡ㉁ࡀ㐺ษ࡟ᯫᶫࡉࢀࡿ⃰ᗘࡀ␗࡞ࡿࡇ

⤖ᯝ࡜ࡋ࡚ࠊAL1 ⣽⬊ࢆ࣐ࢸࣜ࢔ࣝ࡜ࡋ࡚⏝࠸ࡓ࡜ࡀࢃ࠿ࡗࡓࠋࡲࡓࠊPercoll ࡟ࡼࡿ⏕⣽⬊ศ⏬࡟ࡼ

HiC ἲ࡛ࡣࠊ࣒࣍ࣝ࢔࣑ࢻెቃࡢ⃰ᗘ࡜᫬㛫ࠊࡲࡓࡗ࡚࣊ࣃࢺࢧ࢖ࢺ࡜㉥⾑⌫ࡢ⣽⬊ࢆศ㞳ࡍࡿࡇ࡜ࡶ

⣽⬊ࡸ᰾ᢳฟ≀ࡢᘏఙ㏿ᗘࡢ᮲௳᳨ウ࡞࡝ࠊ඲࡚ࡢヨࡳࡓࠋࡑࡢ⤖ᯝࠊ㉥⾑⌫࡜࣊ࣃࢺࢧ࢖ࢺࡢศ㞳࡟ࡣᡂຌࡋࡓࡀࠊ࣊ࣃࢺࢧ࢖ࢺ࡜⬡㉁ࡣྠࡌẚ㔜ࡢᒙ࡟ศ⏬ࡉࢀࡿࡇ࡜ࡀࢃ࠿ࡗࡓࠋࡑࡢࡓࡵࠊ⫢⮚ࢆ࣐཯ᛂࡢຠ⋡࡜⢭ᗘࢆ᪤Ꮡࡢࣉࣟࢺࢥࣝ࠿ࡽᨵኚࡍࡿࡇ࡜࡟ᡂຌࡋࠊ⚽࡛ࡓࢹ࣮ࢱࢆ⏕ࡳฟࡋࡓࠋゎᯒࢹࢸࣜ࢔ࣝ࡜ࡋ࡚⏝࠸ࡿࡇ࡜ࡣ㞴ࡋ࠸࡜ุ᩿ࡋࡓࠋ

࣮ࢱࡢࢤࣀ࣒࢔ࣀࢸ࣮ࢩࣙࣥ⢭ᗘࡣࠊ90%ࢆ㉸࠼ࡿ ḟ࡟ࠊ࢔࣮࣍ࣟࢺࣝࡢୖ⭎෌⏕㒊⏤᮶ࡢᇵ㣴⣽⬊ࡶࡢ࡛࠶ࡾࠊde novo ࢤࣀ࣒࢔ࢵࢭࣥࣈࣜࢆ㐍ࡵࡿ࡛࠶ࡿ AL1 ⣽⬊ᰴࢆ⏝࠸࡚᮲௳᳨ウࢆ⾜࡞ࡗࡓࠋᐇୖ࡛ࡣ༑ศ࡛࠶ࡿࠋ⌧ᅾࡲ࡛࡟ࠊ⣙  Ꮡᅾࡋ㦂ᙜึ࠿ࡽᇵ㣴⣽⬊ࢆ⏝࠸࡞࠿ࡗࡓ⌮⏤࡛࠶ࡿࡀࠊ

ࡓ▷࠸᩿∦ࢆࠊ ࡢࢫ࢟ࣕࣇ࢛࣮ࣝࢻ࡟ࡲ࡛࢔ࢵࢭ㸦㸯㸧ᶞ❧ࡉࢀ࡚࠿ࡽ᫬㛫ࡀ⤒㐣ࡋ࡚࠾ࡾࠊ⥅௦ࡀࣥࣈࣜࡍࡿࡇ࡜࡟ᡂຌࡋࡓࠋᮏ᮶ࡢ࢔࣮࣍ࣟࢺࣝࡢ㐍ࢇ࡛࠸ࡿᇵ㣴⣽⬊⣔⤫࡛࠶ࡿࡓࡵࠊಶయ࠿ࡽᅇ཰

ᰁⰍయᩘࡣ  ᮏ࡜ண᝿ࡉࢀ࡚࠸ࡿࡇ࡜࠿ࡽࠊ࡯࡜ࢇ࡝ࡢᰁⰍయࡢ࢔ࢵࢭࣥࣈࣜ࡟ᡂຌࡋ࡚࠸ࡿ࡜⪃࠼

Live Imaging of Axolotl Digit Regeneration Revealsࡽࢀࡿࠋ⌧ᅾࡣࠊࡇࡇࡲ࡛࡟ᵓ⠏ࡋࡓࢤࣀ࣒᝟ሗࢆ

Spatiotemporal Choreography of Diverse Connectiveᇶ┙࡜ࡋ࡚ࠊ⏦ㄳ⪅ࡢᮏ᮶ࡢ◊✲ㄢ㢟࡛࠶ࡿ఩⨨᝟

Tissue Progenitor Pools. Developmental Cell, 39:ሗࡢ୕ḟඖ෌ᵓ⠏ࡢศᏊᶵᵓࡢゎᯒࢆ㛤ጞࡋࡓ࡜ࡇ

411–423 (2016)ࢁ࡛࠶ࡿࠋຍ࠼࡚ࠊ㠀ࣔࢹࣝື≀࡟࠾࠸࡚ +L& ἲࢆ☜❧ࡋࡓࡇ࡜࠿ࡽࠊ࢘࢕࣮ࣥ኱Ꮫࡢ◊✲⪅࠿ࡽඹྠᘬ⏝ㄽᩥ◊✲ࡢ⏦ㄳࢆཷࡅࠊࣄ࣓࢖࢝㸦Ꮫྡ㸸 ,GLRVHTLXV

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Xenopus forelimb regeneration” Developmentalࢫࡢඹྠ◊✲⪅ࢆ➹㢌ⴭ⪅࡜ࡋࡓࢤࣀ࣒ࣉࣟࢪ࢙ࢡ

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ࢺㄽᩥࠊཬࡧ࢔࣮࣍ࣟࢺࣝࡢ HiC ἲ࡟ࡘ࠸࡚ࡢグ㏙

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ㄽᩥ࡜ࡋ࡚ᢞ✏‽ഛ୰࡛࠶ࡿࠋ

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Froglet Limb Regeneration”

ཱྀ㢌Ⓨ⾲ヱᙜ࡞ࡋㄅୖⓎ⾲
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