Jpn. J. Food Chem. Safety, Vol. 29(3), 2022

179ノート

日本食品化学学会誌、Vol. 29(3), 179-183(2022)
Japanese Journal of Food Chemistry and Safety (JJFCS)

Note

クルクミンの活性は、最初に分散・溶解させる溶媒によって変化する（2022 年 5 月 11 日受付）

（2022 年 8 月 15 日受理）

長野一也 a, b)、前北 光 a)、平井はるな a)、中尾友洋 a, c)、木下圭剛 c)、坂田西野雅之 c)、辻野博文 a, d)、東阪和馬 a, e)、堤 康央 a, f)

慎 c)、a) 大阪大学大学院薬学研究科b) 和歌山県立医科大学薬学部c) 三栄源エフ・エフ・アイ株式会社e) 大阪大学高等共創研究院d) 大阪大学総合学術博物館f) 大阪大学国際医工情報センター

Activity of curcumin depends on initial dispersion solvent(Received May 11, 2022)(Accepted August 15, 2022)

Kazuya Nagano a, b), Hikaru Maekita a), Haruna Hirai a), Tomohiro Nakao a, c), Keigo Kinoshita c), Makoto Sakata c),
Masayuki Nishino c), Hirofumi Tsujino a, d), Kazuma Higashisaka a, e), Yasuo Tsutsumi a, f)a) Graduate School and School of Pharmaceutical Sciences, Osaka Universityb) School of Pharmaceutical Sciences, Wakayama Medical Universityc) San-Ei Gen F.F.I., Inc.
d) Museum of Osaka Universitye) Institute for Advanced Co-creation Studies, Osaka Universityf) Global Center for Medical Engineering and Informatics, Osaka UniversityAbstract
Curcumin (Cur) is the main component of turmeric. Cur performs various physiological actions; however, it is poorly soluble inwater. Because Cur is barely absorbed by the body, its activity is minimal. We have developed an amorphous formulation of Curthat displays improved water solubility and bioavailability. In this study, we analyzed cancer cell cytotoxicity of amorphous Cur andthe conventional formulation (unformulated powders of Cur and commercial formulation of Cur) dispersed in water, to compare thefunctional expression of Cur. In the cytotoxicity assay against HepG2 cancer cells, amorphous Cur exhibited high cytotoxicity, while
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cancer cell cytotoxicity of commercial Cur. This variation may be due to poorly water-soluble reagents, such as inhibitors, being
dissolved in dimethyl sulfoxide (DMSO) prior to dilution with the medium. Thus, we hypothesized that the functional expression ofCur depended on the solvent used for initial dispersion. We evaluated the dispersibility of each Cur formulation in water or DMSO.
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crystal particles in unformulated and commercial Cur, which restricted their solubility in water. Conversely, the Cur formulation
completely dissolved when dispersed in DMSO. To evaluate the cytotoxicity of each Cur formulation in water or DMSO, HepG2
cancer cells were treated with each formulation. In case of the Cur formulation dissolved in water, only the amorphous Cur showedcytotoxicity; in contrast, all Cur formulations dispersed in DMSO showed cytotoxicity. The collective data demonstrate that theF\WRWR[LFLW\RI&XUDJDLQVW+HS*FDQFHUFHOOVGHSHQGVRQWKHVROYHQWXVHGIRUWKHLQLWLDOGLVSHUVLRQ7KHVH¿QGLQJVVXJJHVWWKH
importance of designing an experimental method according to the purpose of the research.

Keywords : クルクミン、溶媒、分散性、生理活性
curcumin, solvent, dispersibility, physiological activity

連絡先： 〒 565-0871大阪府吹田市山田丘 1-6

大阪大学大学院薬学研究科 長野一也・堤 康央

Corresponding author: Kazuya Nagano and Yasuo Tsutsumi, Graduate School of Pharmaceutical Sciences, Osaka University,
1-6 Yamadaoka, Suita, Osaka 565-0871, Japan