ܿ੅঩ၳTR06BōńĮŎŔ୺ဥͺηΦॸ໦ଢ଼΃ρθଲ‫ٮ‬੝͈ōńĮŎŔ୺ဥȁ๱ρασ‫ا‬ȪͼϋΗ·ΠȫͺηΦॸ୺ဥ໦ଢ଼΃ρθไဥōńĮŎŔ́۰ౙȆࣞ௸ͅͺηΦॸ໦ଢ଼̦‫خ‬ෝͺηΦॸͬρασ‫̩̭̳̈́͂ͥا‬ōńĮŎŔ໦ଢ଼̧̦̳́͘ōŦŶĭġŊŭŦġ͈̈́̓։଻ఘ໦ၗ͜‫خ‬ෝ̳́ΗϋΩ·ৗࢹ଼ͺηΦॸ͉ĶĮĲı໦́௶೰̧̦̳́͘ͺηΦॸ͞‫۾‬Ⴒ‫ࣣا‬໤ȄΐβίΙΡ̈́̓ͅഐဥ̧̳́͘ͺηΦॸ͈ழ̵ࣣ͉̽̀͢ͅĲ໦ո‫͈ئ‬ΧͼΑσȜίΛΠ໦ଢ଼̧̦̳́ࣞ͘੗ഽ‫ેݩ‬Ώς΃ġİġˏŶ̉ġၥঊġİġͺηΦॸ໦ၗ୺ဥࡥ೰௖Ȫͼ΂ϋ࢐۟ȼਜ਼௖κȜΡȫōńĮŎŔĩİŎŔĪ́Ĳı໦ոඤͅ໦ଢ଼̦‫خ‬ෝArg

aminoadipic acid

Amino acids standard
10 nmol/mLaminoethanol

Thrbeta-aminoisobutylic acidcarnosinehydroxylysine(Cys)2ornithinehydroxyproline
sarcosine

LysAla beta-Ala

methionine sulfone

anserine
methylhistidine

2-Aminobutylic acidtheanineCys

cystathionineVal

citrulline

Tyraminobutylic acidAsnAsp

ProMettaurineLeu

GlnIleSerGlyGlu

TrpPhe01234His56750 x 3 mm

A: acetonitrile /HCOOH =100 /0.1
B: 100mM ammonium formate
14%B (0-3min)14-100%B (3-10min)
100%B (10-15min)
0.6 mL/min, 40°C
2µL (10nmol/mL, 0.1N HCl)
LCMS-8050 (Shimadzu Corp.)
ESI, Positive/Negative, MRM

8910 minৗၾ໦ଢ଼௡౾ȪŎŔȫ͉ৗၾ͈։̈́ͥͺηΦॸͬ஖఼എͅ࠿੄̧́ͥไဥ࠿੄‫̳́ܕ‬ȃȁ̭͈ไဥŎŔ࠿੄‫ڰͬܕ‬ဥ̳̹ͥ͛ͅȄͼϋΗ·Π͉΃ρθ୺࿝ιȜ΃Ȝ̱͂̀ଲ‫́ٮ‬੝͛̀ͺηΦॸ໦ၗ୺ဥࡥ೰௖ͬ‫ٳ‬อ̱̱̹͘ȃŊůŵųŢťŢġłŮŪůŰġłŤŪť΃ρθ͂ōńĮŎŔ͈ழ̵ࣣͤ͢ͅȄρασ‫͈ا‬๏ॠ̯̦̩̈́Ȅౣশ‫ۼ‬໦ଢ଼̦‫خ‬ෝ̱̹̈́ͤ͘ͅȃ̱̥͜ਲြ༹͉̥̹̈́̽ͅ΃ρθ΍ͼΒΨς΀ȜΏοϋͤ͢ͅȄͺηΦॸ͈ழ̵ࣣ͉̽̀͢ͅĲ໦̞̠͂ΧͼΑσȜίΛΠ໦ଢ଼̦৘̧࡛́ͥ‫ڟ‬૧എ̈́ୋ຦̳́ȃȁυͼΏϋİͼΕυͼΏϋ̦໦ၗ̧̺̫̩́ͥ́̈́ȄϸĮȄϹĮͺηΦॸ։଻ఘ͞ΐβίΙΡ̈́̓ȄͺηΦॸ‫۾‬Ⴒ‫ࣣا‬໤͈͒ഐဥํս̦ࢩ̞̭͂͜අಿ̳́ȃဋၗͺηΦॸ໦ଢ଼ȄΗϋΩ·ৗࢹ଼ͺηΦॸழ଼໦ଢ଼ȄͺηΦॸయ৫ࠏȄͺηΦॸΨͼ΂ζȜ΃Ȝ͈̈́̓ࡄ‫ݪ‬໦࿤͈͒؊ဥ̦‫ܢ‬ఞ̯̳ͦ͘ȃ
Imtakt USA (info@imtaktusa.com)
North America

Imtakt Corp./JAPAN (info@imtakt.com)
Other Countries

TR06BJ-TA16ĶĶਅ႒͈ͺηΦॸͬĲı໦́ōńĮŎŔ໦ଢ଼ŊůŵųŢťŢġ łŮŪůŰġ łŤŪťġ ͈ࡥ೰௖͉ȄͺηΦॸ͈༗঵͞໦ၗ͈̹͛ͅඅ༆ͅ୭̯̞̳ࠗͦ̀͘ȃȁਲြ͈ίτρασ‫༹̠͈̈́͢ا‬๏ॠ̯̦̩̈́Ȅ̱̥͜ไဥ͈ōńĮŎŔͬဥ̞̀ΧͼΑσȜίΛΠ໦ଢ଼̦‫خ‬ෝ̱̹͂̈́ͤ͘ȃȁ࡛শത́ĶĶਅ႒͈ͺηΦॸ̤͍͢႒‫׻‬໤ৗ͈໦ଢ଼̦‫خ‬ෝ̱̹͂̈́ͤ͘ȃĶĶਅͺηΦॸȪດ੔দၳȫ
Intrada Amino Acid, 50 x 3 mm
A: acetonitrile /tetrahydrofuran /25mM ammonium formate /formic acid= 9 / 75 / 16 / 0.3
B: acetonitrile / 100mM ammonium formate = 20 / 80
0 %B (0-2.5min)
0-17 %B (2.5-6.5 min)a
100 %B (6.5-10 min)
0.6 mL/min (6MPa), 35°C, 5 µL (1000nmol/mL)
ESI (SIM, positive)

arginine (Arg, m/z 175.1)
ornithine (Orn, m/z 133.1)
hydroxylysine (Hylys, m/z 163.0)
lysine (Lys, m/z 147.0)
carnosine (Car, m/z 227.1)
anserine (Ans, m/z 241.1)ba) 1-methylhistidine (1-Mehis, m/z 170.1)b) 3-methylhistidine (3-Mehis)

histidine (His, m/z 156.0)2-aminoethanol (EtOHNH2, m/z 62.1)
cystathionine (Cysthi, m/z 223.1)
5-aminolevulinic acid (m/z 132.0 )b caa)b)c)d)

cystine ((Cys)2, m/z 241.0)d-aminobutyric acid ( -Abu)-aminobutyric acid ( -Abu)-aminoisobutyric acid ( -Aiba)-aminobutyric acid (GABA, m/z 104.0)

o-phosphoethanolamine (m/z 142.0)5-aminovaleric acid (m/z 118.1)
glycylproline (Gly-Pro, m/z 173.1)
o-phosphoserine (Ser (P), m/z 186.0)ōńĮŎŔ͈ඳത͉Ȅৗၾ̦൳̲‫ࣣا‬໤͉Ȅ΃ρθ́໦ၗ̳ͥຈါ͈̜̭̳ͥ͂́ȃུ΃ρθ͉Ȅ‫ུܖ‬എ̈́ͺηΦॸ̜́ͥυͼΏϋİͼΕυͼΏϋĩĲĴĲŅŢĪ͞ΨςϋİΦσΨςϋ։଻ఘĩĲĲĸŅŢĪ͜໦ၗ̧̳̭̦̳ͥ͂́͘ȃ̹͘ȄΗϋΩ·ৗ͈ͺηΦॸழ଼໦ଢ଼́ਹါ̈́ΏΑΞͼϋॸ͜໦ଢ଼̧̳́͘ȃ̭͈ఈȄ๱ഛடͺηΦॸ͞୆ఘಎ͈ဋၗͺηΦॸ͈ఉུ༹̩̦́໦ଢ଼‫خ‬ෝ͂ࣉ̢̳ͣͦ͘ȃ̯͉ͣͅȄͺϋΓςϋ͞΃σΦΏϋ
͈̈́̓ΐβίΙΡ͜ͅഐဥ̧́
̳͘ȃ

citrulline (Cit, m/z 176.1)
asparagine (Asn, m/z 133.0)
glycine (Gly, m/z 76.4)
glutamine (Gln, m/z 147.0)
serine (Ser, m/z 106.1)ca) alanine (Ala, m/z 90.0)b) sarcosine (Sar)c) -alanine ( -Ala)a b

aspartic acid (Asp, m/z 134.0)
threonine (Thr, m/z 120.1)
aba) o-methylserine (m/z 120.1)b) allo-threonine (allo-Thr)c) homoserinechydroxyproline (Hyp, m/z 116.0)
proline (Pro, m/z 116.0)
taurine (Tau, m/z 126.0)
methionine sulfone (m/z 182.0)
glutamic acid (Glu, m/z 148.0)-aminoadipic acid ( -Aaa, m/z 162.1)
theanine (m/z 175.1)
cysteine (Cys, m/z 122.0)aba) norvalineb) valine (Val, m/z 118.0)-aminopimelic acid (Apm, m/z 162.1)

aba) leucine (Leu, m/z 132.0)b) isoleucine (Ile)allo-isoleucine (m/z 132.0)
norleucine (m/z 132.0)
methionine (Met, m/z 150.0)
tyrosine (Tyr, m/z 182.0)
phenylalanine (Phe, m/z 166.0)

tryptophan (Trp, m/z 205.0)
thioproline (m/z 134.0)
cysteic acid (Cys(O3H), m/z 170.0)

02.5

57.5

210 min‫ڐ‬ಫ଻͈̞ࣞཅີ̈́΃ρθ΍ͼΒ͂਴඲̈́໦ଢ଼ૄ࠯ਲြ͈ͺηΦॸ໦ଢ଼༹ͅ๤͓̀Ȅུ༹͉ুဇഽ͈̞ࣞ໦ଢ଼༹̳́ȃȁ֊൲௖ழ଼ͬͺηΦॸ͈ਅ႒͞໦ଢ଼ۜഽ̈́̓ͅ؊̲̀་ࢵ̧̳̭̳ͥ͂́͘͜ȃġ ̹͘Ȅ΃ρθ΍ͼΒͬ་ࢵ̳̭ͥ͂ͤ͢ͅȄୈྟ໦ၗ಼ࣞ͞௸໦ଢ଼͜‫خ‬ෝ̱̱̹͘ͅȃȁ։଻ఘͬ‫̞̈́͘܄‬ͺηΦॸ̜́ͦ͊Ĳ໦ոඤ͈໦ଢ଼͜‫خ‬ෝ̳́ȃȁ̯͉ͣͅȄࣞޭ଻ΐβίΙΡ̈́̓Ȅ೒ુ͈‫ݙ‬௖κȜΡ͉́ࣾඳ̈́ͺηΦॸࠏ‫ࣣا‬໤͜ͅഐဥ̧̳́͘ȃ৘দၳȪ̥̤̾୯ȫ͈ஜੜၑ͂໦ଢ଼႕
ಒ੄Dried bonito
hot water
extracting, centrifugationੰ౩ฒArg (m/z 175.1)

Extract

Lys (m/z 147.0)

5% sulfosalicylic acid(1:1, vol)mixingcentrifugation(>12krpm, >5min)
Supernatant (Injection Sample)

Car (m/z 227.1)
Ans (m/z 241.1)(Cys)2 (m/z 241.0)
Asn (m/z 133.0)
Gly (m/z 76.0)
Gln (m/z 147.1)

A: ACN / THF / 25mM HCOONH4 / HCOOH= 9 / 75 / 16 / 0.3 (v/v/v/v)
B: ACN / 100mM HCOONH4= 20 / 80 (v/v)
0%B (0-3min), 0-17%B (3-9min)
100%B (9-15min)
0.6 mL/min (9MPa), 35°C, 5µL
ESI, positive, SIM

Ser (m/z 106.0)
Ala (m/z 90.0)
Asp (m/z 134.0)
Thr (m/z 120.1)
Pro (m/z 116.1)(%, 1mM)
100Glu (m/z 148.0)
Val (m/z 118.1)
Leu, Ile (m/z 132.1)(%, 100µM)
100

His (m/z 156.0)

Met (m/z 150.0)

5050

Tyr (m/z 182.1)
Phe (m/z 166.1)
Trp (m/z 205.1)

002

460
8

10121416 min760৘দၳͬ໦ଢ଼̳ͥાࣣȄͺηΦॸ͈ಒ੄͂ੰ౩ฒௌै̦ຈါ̳͂̈́ͤ͘ȃͺηΦॸಒ੄͉ͅদၳͅ؊̲̀କ̱̩͉͜ॸ଻କဣͬဥ̞̳͘ȃੰ౩ฒௌै͉ȄĶĦΑσγ΍ςΙσॸ̱̩͉͜ıįĵŏً‫؂‬ளॸͬ൝ၾഞ‫̭̳͂ͥح‬́ΗϋΩ·ৗ̦௸̥͞ͅާਬ̳͈ͥ́Ȅ‫׿‬૤ͤ͢ͅ۰ౙͅੜၑ̧̳́͘ȃං̹ͣͦॸ଻͈ષୄ‫ס‬̷͉͈͘͘΃ρθͅಕව̧̳̭̦̳ͥ͂́͘ȃऒ଎͉̥̤̾୯ͬ෎କ́ಒ੄̱ȄΑσγ΍ςΙσॸ́ੰ౩ฒ̱̹͈ͬ͜΃ρθಕව̱̹໦ଢ଼႕
̳́ȃȁυͼΏῧͼΕυͼΏϋȪ։଻ఘȫ͜ౣশ‫ۼ‬́໦ၗ̧̤́̀ͤȄ̯ͣͅ‫͈଻ܖ؂‬ΪΑΙΐϋ‫܄‬ခၾ̦අ։എͅఉ̞̭̥̱̹͂ͩͤ͘͜ȃ೰ၾ଻͂ۜഽ͉ȄͺηΦॸࡥခ͈ࢹ௮͞ŎŔ଻ෝͅջం̱̳͘ȃࣞۜഽ໦ଢ଼͉ͅΠςίσঅਹޭŎŔ͞ŕŐŇĮŎŔĭġŇŕĮŎŔ̦̈́̓ଔ੻̯̳ͦ͘ȃ̹ࣞ͘ୈഽ೰ၾ̦ຈါ̈́ાࣣ͉ȄచયͺηΦॸ͈൳պఘͬඤ໐ດ੔̱͂̀ঀဥ̳ͥຈါ̦̜̳ͤ͘ȃ

ĳıਅດ੔ဋၗͺηΦॸ͈ଔ੻໦ଢ଼ૄ࠯͂໦ၗι΃ΣΒθ
Intrada Amino Acid, 100 x 3 mm
A: acetonitrile / formic acid = 100 / 0.3
B: acetonitrile / 100mM ammonium formate = 20 / 80
20%B (0-4 min), 20-100%B (4-14 min), 100%B (14-16 min)
0.6 mL/min (5MPa), 37°C, 5 µL (acidic solution)
MS: SingleQ /TripleQ/ TOF-MS / FT-MS (ESI. positive)

23

1
H3C

ON

H3C

H3C

CH3m/z 156.0

2N

310 min

HN
N

L-histidine

H3C
OH

NH2

L-leucine

H3C

O

H3C

OH
NH2

Intrada Amino Acid, 150 x 3 mm
A: methanol / water / formic acid= 85 / 15 / 0.3
B: acetonitrile / 100mM HCOONH4= 20 / 80
12-13 %B (0-12 min)
100 %B (12-15 min)
0.4 mL/min (11 MPa)
35°C, 5µL (0.1N HCl)
ESI (positive, m/z 132.0)

NH2OOH
NH23-methyl-L-histidine

m/z 170.1O

OH

allo-L-isoleucine

OHN

H3C

NH21-methyl-L-histidine

7.5űŉȆͼ΂ϋ‫ޑ‬ഽͬષ̬Ȅခ‫ܥ‬ဣ෾ෛഽͬ‫̬̀ئ‬ဣ੄ĲııĦŃ͉΃ρθ஄૆͜࠳͇̞̳̀͘
ಕවদၳဣ‫́ئ࠯ૄ଻ॸ͉ס‬ͺηΦॸͬုͼ΂ϋ‫ا‬ȪĳĦΆॸȄıįĳŏġً‫؂‬ளॸȄıįĲŏġ‫͈̓̈́ॸ؂‬ಕවဣ෾ȫዙॠ໤̦ఉ̞ાࣣࣞۜ͞ഽ̦ຈါ̧͉̈́͂
ŎŔİŎŔĭġŕŐŇĮŎŔĭġŇŕĮŎŔ̦ଔ੻̯̳ͦ͘

Intrada Amino Acid, 100 x 3 mm
A: methanol / water / formic acid= 85 / 15 / 0.3
B: 100mM ammonium formate
55-70 %B (0-10 min), 100 %B (10-12 min)
0.4 mL/min (8MPa), 50°C
5µL (1mM, 0.1N HCl)OMS (ESI, positive)
N5ုͼ΂ϋ‫̹̱ا‬ͺηΦॸͬॸ଻‫ئ‬Ȅਜ਼௖Ȇͼ΂ϋഎͅ‫ݟ‬಍υͼΏϋ։଻ఘġĩĲĴĲŅŢĪġ͈໦ଢ଼႕ΪΑΙΐϋ͈ιΙσ‫ا‬։଻ఘ͈໦ଢ଼႕

1ͺηΦॸਅ႒̦ఉ̞ાࣣ͉ͅĲııŹĴŮŮġ̦ଔ੻̯̳ͦ͘OOHNH25743

7.5L-isoleucine

1012.5 min747։଻ఘ͈໦ၗ͉ͅĲııŮŮոષ͈ಿ̞΃ρθ̦ଔ੻̯̳ͦ͘ȃȁུ̯ͣͅ΃ρθ͈໦ၗࡔၑ̜́ͥȶਜ਼௖ȼ΃Ι΂ϋ࢐۟ȷͅ௱ͤȄ੝‫ܢ‬ဣ෾ͬ஖఼଻͈։̈́ͥιΗΦȜσͅఢ̢Ȅਜ਼௖ȼűŉĬͼ΂ϋ‫ޑ‬ഽΈρΐ΀ϋΠ͈࢙෻ͬडഐ‫̭̳́͂ͥا‬։଻ఘ໦ၗ̦ယօ̳̈́ͤ͘ͅȃུ༹͉ȄไဥōńĮŎŔͬဥ̞̹ͥ͛Ȅਲြ͈ͺηΦॸ໦ଢ଼୺ဥΏΑΞθͤ͢͜ুဇഽ̦̩ࣞ̈́ͥ၌ത̦̜̳ͤ͘ȃ̹͘ৗၾ໦ଢ଼௡౾͈ඳത̜́ͥ։଻ఘ͈໦ଢ଼ͅ‫̱̀͜۾‬ȄͺηΦॸͬည൵ఘ‫΃̩̭̳̈́͂ͥا‬ρθུြ͈࿨‫̜ͥ́ڬ‬ȶ໦ၗȷͬယօͅ৘̧࡛̳́͘ȃ

3΋ςϋయ৫໤͂‫۾‬ႲͺηΦॸ
CH31OOOO1POOHOO

CH3಼ࣞ௸໦ଢ଼႕

CH3 1,2-dimyristoyl-sn-glycero-3-phosphocholine+ CH3N
CH3OCH3OO

3

3[M+H]+ m/z 636.4

H3C N

CH3 Cl+5HON[M+H]+ m/z 104.1

H3CSO

HOO

910O

HO

[M+H]+ m/z 184.1

N[M+H]+ m/z 489.1

PHOOH

OPPOO
OH OHm/z 125.1

AlaON

NGln

OH

Gln-Ala Ala-Gln

NH2

Leu+S

HO

15 minO

CH3HO2OOH

31

NH2

5 H3CO

2

3[M+H]+ m/z 182.0[M+H]+ m/z 198.1[M+H]+ m/z 168.1[M+H]+ m/z 154.1

0

NH2[M+HCOO]- m/z 227.0[M-H]- m/z 167.0

54

HOL-tyrosine

3-methoxytyramine

HO4 HOOH

HO6HOHONH2

methylselenocysteineSeselenomethionine

COOH

818

SeSe

selenocystine 100 x 3 mm

ONH2NH2150 x 3 mm
A: acetonitrile / formic acid = 100 / 0.3
B: 100mM ammonium formate
10-100 %B (0-10min), 100 %B (10-15min)
0.5 mL/min (6 MPa), 37°C
20 µL (10µg/mL, 1% HCOOH). ESI, SIM[M-H]- m/z 182.0[M+ACN+H]+ m/z 239.0[M+H]+ m/z 336.9

024

68

1012 min996๱ഛட߿ͺηΦॸ817

421OOHNH2O
H3CH3C̮ಕփུୋ຦͉ȄōńĮŎŔ୺ဥ͈ͺηΦॸȆ‫۾‬Ⴒ‫ࣣا‬໤໦ଢ଼ဥ΃ρθ̳́ȃġ Ŗŗ͞ņōŔŅ̈́̓ͥ͢ͅ࠿੄༹༷͉ȄάȜ·͈൳೰̦ະ‫ږ‬৘̵̧̳͈̤̳̳͂̈́ͤ́͛́ͭ͘͘ȃ໦ଢ଼ۜഽ͉Ȅঀဥ̯ͦͥৗၾ໦ଢ଼௡౾ͅջం̱̳͈́͘Ȅ໦ଢ଼࿒എ͞ຈါۜഽͅഐ̱̹ōńĮŎŔ̮ͬဥփ̩̺̯̞ȃུୋ຦̮ͬঀဥ͈ष๊͉֚ͅ‫ࣣا‬໤͂൳အͅȄஜੜၑ༹༷Ȅ΃ρθ΍ͼΒȄΈρΐ΀ϋΠૄ࠯Ȅ࠿ၾ஌̈́̓Ȅŉőōń໦ଢ଼ૄ࠯͈डഐ‫̸̷͈ͦͦͬا‬ιΕΛΡ̮͂ͅ৘ঔ̳ͥຈါ̦̜̳ͤ͘ȃͺηΦॸழ଼໦ଢ଼̈́̓Ȅদၳ͈ஜੜၑ๊༹༷͉֚എ̈́‫ح‬କ໦༹ٜ̮ͬ४ચ̩̺̯̞ȃ

COOH
NH2

A: ACN / HCOOH = 100 / 0.3
B: ACN / 100mM HCOONH4= 20 / 80
20%B (0-4 min)20-100%B (4-14 min)
100%B (14-16 min)
0.6 mL/min (5 MPa), 37°C
5 µL (1% HCOOH)
ESI, SIMOH

dopamine

15 minNH2HOOC

NH231015 minΓτΦͺηΦॸCOOH

໦ଢ଼ૄ࠯͉̤࿚̵̞ࣣ̩̺̯̞ͩ 45Leu-His
10816

3,4-dihydroxy-L-phenylalanine(L-DOPA)

65SeOOHO

homovanillic acid(HVA)

60 secIntrada Amino Acid, 150 x 3 mm
A: ACN / HCOOH = 100 / 0.3
B: ACN / 100mM HCOONH4 = 60 / 40
70-100%B (0-6min), 100%B (6-15min)
0.6 mL/min (9 MPa), 37°C
10 µL (100nmol/mL, 1%HCOOH)
ESI, posive, SIM

His-Leu
03,4-dihydroxyphenyl acetic acid (DOPAC)

48

His

S-(5'-adenosyl)L-methionine (SAM)N

36

Glu-GluOH

ΙυΏϋయ৫ࠐႹHO

24

τΠυΐβίΙΡ

+Intrada Amino Acid , 100 x 3 mm
NH2
A: acetonitrile / formic acid = 100 / 0.3
B: acetonitrile / 100mM ammonium formate = 20 / 80
10-100%B (0-10min), 100 %B (10-15min)
0.4 mL/min (3 MPa), 37°C, 10 µL (5µg/mL, 2% HCOOH) , ESI (positive, SIM)

112GluH3C N

HO11NOH

NH2taurine

0choline chloride[M+H]+ m/z 399.1

H3COO

CH3CH3NH210OH

N
H

m/z 133.1m/z 176.1m/z 162.1

10cytidine
5'-diphosphocholine[M+H]+ M/Z 104.25OH
NH2citrulline

H2N

CH3+ CH3N
CH3O

CH3+ CH3N
CH3O

N11OO
S OH

phosphocholine90

H2NO-OH8OOH

NH2L-methionine[M+H]+ m/z 150.1O

N,N-dimethylglycine

O7NNH2[M+H]+ nm/z 385.1

carnitine

OH2N

S-(5'-adenosyl)-L-homocysteine (SAH)

NN

7NH2O

6H3CNOH O

CH2CH3 O5OH

S

N+H3C

OH

CH3O

HO

trimethylglycine(betaine)

CH3 O
H3C N+acetylcholine

6[M+H]+ m/z 118.0

4OO

CH3[M+H]+ m/z 146.1

8

CH2

1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine[M+H]+ m/z 678.5

42O

CH32NH2PO
OH

O
O

10 x 2 mm

A: CH3CN /HCOOH = 100 /0.1
B: 100mM HCOONH415-100 %B (0-0.8 min), 100 %B (0.8-1.0 min)
0.4 ml/min (1.6 MPa), 35°C, 1 µL (0.1N HCl)ornithine
ESI, positive

31-aminocyclopropane
carboxylic acidOH 2-aminoisobutyric acid(2-methylalanine)

NH2m/z 102.01-aminocyclopentane
O 2carboxylic acid(cycloleucine)OH
NH2m/z 104.0m/z 130.1m/z 212.10HOHO

257.5OOH
H2N CH31L-methyldopa

10 min771

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