ˤˬ˨˟΃ρθȁͼϋΗ·Π
Highly durable C1 phase஠ఉࢢ଻Ώς΃ၥঊࠂȁĴġЂ̉ळࢢࠂȁˍĴ̊̉ιΙσΏςσ‫ܖ‬ġĩŖŔőĮōĲĴĪűŉġĲįĶĮĺస‫̹̱ק‬ఛॸ଻˟ˍࡥ೰௖Άॸ͞ŕŇł͈̈́̓ॸ଻֊൲௖́‫ح‬କ໦ٜ̱̩̞ͅ૧ܰńĲࡥ೰௖ࣞޭ଻̥ͣ೩ޭ଻‫ࣣا‬໤́͘ࢩํսͅచ؊ŐŅŔ΃ρθ́ဣ੄̱̩̞ͅডဣ଻‫ࣣا‬໤͈໦ଢ଼ŐŅŔ͉͂։̈́ͥ໦ၗ஖఼଻ঀ̞ੳ਀͈ၻ̞ࣞၑა౲ˏЂ̉ၥঊ΃ρθAcid stability (0.1% TFA)

Acid stability (0.5% HCOOH)
Traditional C1(butylbenzoate)

100
Nucleic bases and nucleosides
250 x 3 mm
0.5% HCOOH aq.
0.4 mL/min, 37°C, 260nm

80

0.1% TFA

60
40

Traditional C124hours

2024hours
0.5% HCOOH

0020

40

60

80

100

120hours
0510min

0510min

A29Tizanidine (muscle-relaxant) impurityClNHNHNN

50 x 3 mmA: 10mM HCOONH4
B: 50mM HCOONH4 / ACN= 80 / 20
0 - 10%B (0 - 10min)
0.4mL/min (5MPa), 37°C
2 µL (0.1mg/mL, DMSO)
320nm

N

S

tizanidine

Brand-XB C18024

68 minA27ུୋ຦͈අಿ
ഥൡഎ̈́ńĲ΃ρθ͉Ȅକࠏ֊൲௖ͬဥ̧̞̹͈͂ఛ‫ݛ‬଻Ȅඅͅఛॸ଻ͅඳത̦̜̱̹ͤ͘ȃŖůŪŴŰůġ ŖŌĮńĲ΃ρθ͈‫ٳ‬อͅष̱͉̀Ȅġ ŊůŵųŢťŢ͞ŔŤũŦųŻŰ΃ρθ̈́̓ξΣȜ·̈́ୋ຦‫ٳ‬อ̽̀͢ͅ෽̹ͩͦ૧ܰ̈́ࡥ೰௖୭ࠗܿ੅ͬ؊ဥ̱̱̹͘ȃȁ̷͈ࠫ‫ض‬Ȅ‫ح‬କ໦ٜͅచ̱̀೷ࢯ଻͈̞ࣞńĲນ࿂ࢹ௮ͬ‫ڕ‬ං଼̳̭̱̱̹ͥ͂ࢗ͘ͅȃńĲࡥ೰௖͉ŐŅŔͅ๤͓̀மକ଻̦೩̞฽࿂ȄΏς΃ນ࿂͈ޭ଻‫ै̩ޑ̦ܖ‬ဥ̳̹ͥ͛ȄŐŅŔ΃ρθ͉͂։̈́ͥ஖఼଻̦อ‫̯ͥͦܞ‬ાࣣ̦̜̳ͤ͘ȃȁऒ଎͉́Ȅਲြ͈ŐŅŔ́࠿੄̧̥̹́̈́̽ະ੗໤άȜ·̦໦ၗ࠿੄̯̞̳ͦ̀͘ȃ̹͘ŖůŪŴŰůġ ŖŌĮńĲ΃ρθ͉ȄŐŅŔ͉́ဣ੄̦ࣾඳ̈́ࣞமକ଻໤ৗͅచ̱̀͜Ȅไဥഎ֊൲௖ͬဥ̞̹໦ଢ଼ͅ‫خ‬ෝ଻̦ࡉ੄̵̳͘ȃŐŅŔ΃ρθͬ༞̳ۖͥဥഷͅ‫ݙ‬௖κȜΡ͈యນ̜́ͥńĲĹ΃ρθͬဥ̧̞̹͈͂ဣৗ͈༗঵͉Ȅ̤͜ͅமକഎ௖ࡽैဥ͈̳ͥ́͢͜ͅȃ̱̥̱൳শͅ‫ऺܖ‬ນ࿂͈ޭ଻‫୓ͥ͢ͅܖ‬ഩഎ௖ࡽैဥ̈́̓͜‫ܙ‬ဓ̱̞̹̀ͥ͛Ȅဣৗ͈डਞഎ̈́༗঵͉̭ͦͣໝତ͈໦ঊ‫ۼ‬௖ࡽैဥ͈௙გ̱͂̀ࠨ̳ͤ͘͘ȃȁ̱̹̦̽̀‫ڎ‬௖ࡽैဥ͈‫ܙ‬ဓၚ̦։̈́ͦ͊Ȅࡥ೰௖‫͈́ۼ‬༗঵Ȇ໦ၗඅ଻͜་‫̳̱͘ا‬ȃͺσ΅σङಿ͈ౣ̞ńĲ΃ρθ͉Ȅமକഎ༗঵͉ńĲĹ΃ρθͤ͢͜઀̯̞͈͈͜Ȅဣৗ̦‫ऺܖ‬ນ࿂ͅ୪߃̳̹ͥ͛ͅ୓ഩഎ௖ࡽैဥ͉‫ͅݙ‬ఱ̧̧̩͉̹̳ͣ͘ȃġ̷͈ࠫ‫ض‬ńĲ΃ρθ͉ȄńĲĹ΃ρθ͉͂։̈́ͥ༗঵Ȇ໦ၗඅ଻ͬা̱̳͘ȃ
A36‫ࣣا଻ܖ؂‬໤OHClN

CH3H3Ceperisone (antispasmodic)

75 x 4.6 mm
A: water / formic acid= 100 / 0.1
B: acetonitrile / formic acid = 100 / 0.1
0-50%B (0-15min)
1 mL/min (7MPa), 37°C
5 µL (0.05mg/mL, DMSO), 260nm
0510‫ࣣا଻ܖ؂‬໤ͬΆॸॸ଻֊൲௖́໦ଢ଼̳ͥાࣣȄńĲĹ΃ρθ͉́ဣৗ͈ٜၗેఠ͈ଷࢄ̦ඳ̱̩ȄήυȜΡ̈́άȜ·̭̦̜̳̈́ͥ͂ͤ͘ͅȃġ ńĲ΃ρθ͉ιΙσς΄ϋΡ͈ഐഽ̈́மକ଻ͅ‫̢ح‬ȄΏυ΅΍ϋȪޭ଻‫ܖ‬ȫဇြ͈୓ഩഎ௖ࡽैဥͤ͢ͅȄဣৗٜၗેఠ̦ଷࢄ̱̳̩̈́ͤ͞Ȅၻࢡ̈́άȜ·ࠁેͬဓ̢̳͘ȃŖůŪŴŰůġ ŖŌĮńĲ΃ρθ͉Ȅॸ଻֊൲௖́͜ς΄ϋΡ̦‫ح‬କ໦ٜ̱̩̞ͅນ࿂ࢹ௮̤̈́̽̀ͤͅȄΆॸॸ଻‫͈́ئ‬໦ଢ଼̞ࣞͅఛ‫̳̜̦ͤ͘଻ݛ‬ȃไဥഎ̈́ńĲĹ΃ρθ́ॸ଻֊൲௖ͬဥ̞̀άȜ·ࠁે͞໦ၗ͈‫ه‬ఴ̦อ୆̧̱̹͂ȄŖůŪŴŰůġŖŌĮńĲ΃ρθ͈‫خ‬ෝ଻̦‫ܢ‬ఞ̯̳ͦ͘ȃ

15 minCH3CH3CH3H3CΠςΞσβϋ

CH3CH3Ξσβϋ႒͉மକ଻̦̩ࣞȄ̫͂ͤͩΠςΞσβϋ͉ńĲĹ΃ρθͬဥ̞̀͜๱କࠏ֊൲௖̞́̈́͂ဣ੄̵̧́ͭ͘ȃŖůŪŴŰůġ ŖŌĮńĲ΃ρθ͉Ḙ͈̠̏̈́͢ޭ͛̀மକ଻͈̞ࣞ‫ࣣا‬໤๊֚ͬഎ̈́କĮခ‫ܥ‬ဣ෾ࠏ́ဣ੄̧̭̦́ͥ͂අಿ̳́ȃŖůŪŴŰůġ ŖŌĮńĲ΃ρθ͉Ȅࣞޭ଻‫ࣣا‬໤̺̫̩́̈́೩ޭ଻‫ࣣا‬໤ͅచ̱̀͜࿹̹ͦ؊൞ͬা̱̳͘ȃsqualene

150 x 4.6 mm
A: acetonitrile / water = 70 / 30
B: acetonitrile
25-100%B (0-15min)
0.8mL/min (7MPa)
37°C, ELSD
1 µL (5 mg/mL,THF)
CH3

H3C

CH3CH3CH3CH3CH3CH3squalane0έΗσॸ΀ΑΞσ
2
11OO

35dibutyl phthalate
DBP
4OOOOOO

101520 min

5O2O

C9H19OC9H196O

345610ୋ຦ૂ༭

2030 minOO

C10H21OC10H21O

diisononyl phthalate
DINP

70-100%B (0-30min)

0O

di-2-ethylhexyl phthalate di-n-octyl phthalate
DEHP
DNOP

51OO

50-100%B (0-20min)

0A35

O

butylbenzyl phthalate
BBP

615 min

OO

34

10O

2O

5diisodecyl phthalate
DIDPέΗσॸ΀ΑΞσ͈໦ଢ଼̤̞̀ͅȄńĲĹ͈̠̈́͢ͺσ΅σࡥ೰௖͉ಿङͺσ΅σ‫͈ܖ‬໦‫ܚ‬ङͬ໦ၗ̱̱̠̭̥̀͂ͣ͘೰ၾ଻ͅඳ̦̜̱̹ͤ͘ȃŖůŪŴŰůġŖŌĮńĲ͉ιΙσࡥ೰௖̜́ͤȄς΄ϋΡ͈ͺσ΅σङಿ̦ౣ̞̹͛ͅȄ໦‫̹̱ܚ‬ͺσ΅σङ։଻ఘ͈ে༆ෝ͉̜
ͤ͘࿹̵̞ͦ̀ͭ͘ȃȁ̱̥̱Ḙ͈̏೩̞෇েෝͬ‫ͅݙ‬၌ဥ̳ͦ͊Ȅङಿ̮͂ͅĲུ͈άȜ·̧̭̦̳͂͛ͥ͂́͘͘ͅȃέΗσॸ΀ΑΞσ͈೰ၾ໦ଢ଼ͅŖůŪŴŰůġ ŖŌĮńĲ̦૧̹̈́‫خ‬ෝ଻ͬఽ̧̳͘ȃ150 x 4.6 mm
A: water / AcOH = 100 / 0.1
B: acetonitrile / AcOH = 100 / 0.1
0.8 mL/min (9MPa )37 °C, 260nm
2 µL (1 mg/mL, DMSO)
A34৽̈́ॽအȇȁၥঊࠂȇĴŰųĶЂ̉ȄġळࢢࠂȇĲĴ̊̉Ȅġࡥ೰௖ȇιΙσ‫ܖ‬ĩŖŔőġōĲĴĪȄȁġఛգȇĳĶŎőŢġĩŉŕĻĶıŎőŢĭġŖőĻĲııŎőŢĪġ

΃ρθྴ

(3µm)
(5µm)

΃ρθඤࠂ

΃ρθಿ

1mm, 1.5mm, 2mm, 3mm
4.6mm, 6mm, 10mm
20mm(5µm), 28mm(5µm)

10mm, 20mm, 30mm
50mm, 75mm, 100mm
150mm, 250mm, 500mm

΄ȜΡ΃ρθ΄ȜΡγσΘȜ΃ȜΠςΛΐ΃ρθ

ȶη·υİ΢Φ΃ρθȷġȶιΗσέςȜ΃ρθȷ̞͉̤̾̀ͅ࿚̵̞ࣣ̩̺̯̞ͩȃ

BRUK0A-SK01