Tao Su
2020 May 20thWTW OxiTop® システム アプリケーションレポート
Dr. Tao Su
Xylem Japan Product Manager, Wastewater Segment, North Asia
はじめに
過去30年間、WTW OxiTop®は、生物化学的酸素要求量（BOD）の測定と高度な呼吸活性測定試験の分野で定着した用語である。これまでに、20万個を超えるOxitop
ヘッドが世界中で販売された。これらの製品は、業界だけでなく科学分野でも大きな役割を果たしてきた。
Google Scholarで公開された論文を「Oxitop」というキーワードで検索すると、4380件の結果が得られる。特に、最近の10年間で論文数が大幅に増加した（図1）。

を測定する。圧力低下は基本的な呼吸の作用によるものである。**CO2 は、NaOHにより吸収される。

C（有機物） + O2 (ガス) → CO2 (ガス)+ H2O
酸素利用速度（OUR）と二酸化炭素の発生の計算は、圧力降下の測定に基づいている。圧力降下はOxitopヘッドのデータロガー機能で日毎に記録される。呼吸活性測定試験中の典型的な圧力降下を図2に示す。

図2 呼吸活性測定試験中の典型的な圧力降下
図1 Google ScholarでOxitopというキーワードで検索した際に表示された論文数

OxiTop®システムはBOD測定だけでなく、呼吸活性測定試験においてもより多くの利点が実証されている(Roppola et al. 2007)(Silveira et al. 2019)。本報では、測定原理、主なアプリケーションを紹介する。

主な特長
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確実な結果を得られる扱いやすさ、BOD測定結果を直接表示
Bluetoothによるワイヤレス測定
最大圧力範囲：1500 hPa、最大測定期間：180日
OECD/微生物学に準拠した生物学的分解性の測定 DIN ISO 29 408 / ISO 9408 / OECD 301 Fに準拠した実験手順

測定原理
OxiTop®システムは、圧力センサーを利用して、一定温度下において密閉容器内の気体の位相圧力変化（減圧）www.xylem-analytics.asia© 2020 Xylem

OURや二酸化炭素の変化は、物質のモル数と理論的な酸素消費量の方程式による圧力降下の関係から求めることができる（Lueders 2010）：

ΔP =𝑛𝑅ΔT𝑉

ΔP – 圧力降下 (kPa),n – 物質のモル数 (kモル)
ΔT – ガス温度の変化 (ºK)
R – 一般的なガス定数 (8.134 kJ/モルe· ºK)
詳細な嫌気性計算式は、WTWアプリケーションノート
（Lueders 2010）にある。

アプリケーション
OxiTop®システムには多くの機能があり、研究者に幅広い分野で使用されている。直近15年間に発表された
OxiTopに関する論文をレビューし、主に生分解試験アプリケーションを列挙したものを表1に示した。モデル
チェンジした生物学的分解試験用のOxitopシステムの写真を図3に示した。

Tao Su
2020 May 20th
上位3つのアプリケーションは、木材防腐剤（PHB、PHAを含む）、土壌呼吸活性分析、油の分解性である。
表1

直近の15年間に発表されたOxiTop® の生分解分野のアプリケーション

生分解
木材防腐剤, PHA, PHB

土壌

油

医薬品
廃棄物
界面活性剤
揮発性疎水性物質
有機化合物
プラスティック
揮発性炭化水素
生物凝集剤
包装材料
不織布
生体高分子
泡沫
ペルフルオロオクタンスル
ホン酸塩
キチン質
ナフタレン
コラーゲンハイドロゲル
カチオン性および両性原料
自然風化した車のタイヤ
コリンアミノ酸
農薬
シリコン
革

参考文献
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図3 OxiTop® 生分解性試験セット

Tao Su
2020 May 20th
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