Product Datasheet

PepPool: SARS-CoV-2 (SNMO), human
Product code:3622-1

Contents:

The SARS-CoV-2 SNMO defined peptide pool contains 47 peptides fromthe human SARS-CoV-2 virus. The peptides are derived from the spike (S),nucleoprotein (N), membrane protein (M) and open reading frame (ORF)3a, and ORF-7a proteins (O). The purity of the synthetic peptides range
from 60-99%.

Applications:

The peptide pool is recommended for enumeration of cytokine secreting
T cells specific for SARS-CoV-2 S, N, M, and O proteins with ELISpot/FluoroSpot. The peptide pool has been validated using human PBMC fromCOVID-19 convalescent individuals previously PCR-confirmed as SARSCoV-2 positive.

Instructions:

Sterile handling is recommended. Dissolve the lyophilized peptide pool byaddition of 40 μl DMSO to the vial. Then add 85 μl PBS, mix and aliquoteand store at -20°C or below. This stock solution will have a concentrationof 200 μg/ml of each peptide.
Dilute the stock solution 1:100 in cell culture medium to obtain 2 μg/ml
of each peptide in the cell culture. Use the peptide pool in ELISpot andFluoroSpot assay for stimulation of 250,000-500,000 cells per well. Use thediluted peptide solution fresh.

Storage:

Shipped at ambient temperature. Store frozen at -20°C or below uponreceipt. After reconstitution, store aliquotes at -20°C or below. We
recommend the aliquots not be refrozen after initial use.

Quantity:

One vial with 25 ug of each peptide.
Reference: Peng Y, et al. Broad and strong memory CD4+ and CD8+ T cells
induced by SARS-CoV-2 in UK convalescent COVID-19 patients. NatureImmunology, vol 21, Nov 2020.

Note; for research use only.
Mabtech shall not be liable for the use or handling of the product or forconsequential, special, indirect or incidental damages therefrom.
Mabtech AB (Head Office)SwedenTel: +46 8 716 27 00
mabtech@mabtech.com

Updated on 2022-11-22

Mabtech, Inc.
USA
Tel: +1 513 871-4500
mabtech.usa@mabtech.com

CERTIFIED
ISO 9001

CERTIFIED
ISO 13485

Quality management systems

Medical devices
Quality management systems

Developed and manufactured by MABTECH AB, Sweden,
whose quality management system complies with thestandards ISO 9001:2015 & ISO 13485:2016.

www.mabtech.com

Peptides included in PepPool: SARS-CoV-2 (SNMO), human
Peptide1234567891011121314151617181920
21
2223
24
25
2627
28
2930
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47

ID
S-34
S-39
S-42
S-43
S-71
S-77
S-90
S-91
S-103
S-106
S-145
S-150
S-151
S-161
S-174
S-235
S-240
S-242
NP-1
NP-2
NP-12
NP-15
NP-16
NP-46
NP-47
NP-48
NP-50
NP-51
M-19
M-20
M-21
M-23
M-24
M-28
ORF-3a-20
ORF-3a-27
ORF-3a-28
ORF-3a-30
ORF-7a-2
ORF-7a-7
ORF-7a-10
N-E-01
N-E-03S-E-19N
NNSequence
CTFEYVSQPFLMDLEEFVFKNIDGYFKIYS
KHTPINLVRDLPQGF
NLVRDLPQGFSALEPYAWNRKRISNCVADYGVSPTKLNDLCFTNV
GGNYNYLYRLFRKSNYLYRLFRKSNLKPFEVVLSFELLHAPATVCGPKKSTNLVKNKCVNSVTTEILPVSMTKTSSTECSNLLLQYGSFCNLLLQYGSFCTQLNRNFSQILPDPSKPSKRTDEMIAQYTSALLAGGINASVVNIQKEIDR
LIDLQELGKYEQYIYEQYIKWPWYIWLGF
MSDNGPQNQRNAPRITF
NQRNAPRITFGGPSDSTGDQIGYYRRATRRIRMKDLSPRWYFYYLLSPRWYFYYLGTGPEAGLAFFGMSRIGMEVTPSGTWGMEVTPSGTWLTYTGAIKTWLTYTGAIKLDDKDPNFPNFKDQVILLNKHIDAYKLLNKHIDAYKTFPPTEPKLLESELVIGAVILRGHLRGAVILRGHLRIAGHHLGRLRIAGHHLGRCDIKDLPKPKEITVATSRTLSYYKLTSRTLSYYKLGASQRVAIGNYKLNTDHSSSSDNIAYFLCWHTNCYDYCIPYKDCVVLHSYFTSDYYQLYYFTSDYYQLYSTQLSTDTGVGVEHVTFFIYNKIVDEPEEHLITLATCELYHYQECVRFHPLADNKFALTCFSTQF
DGVKHVYQLRARSVSPKLILLNKHIDMEVTPSGTWLQLIRAAEIRASANLAATKSPRWYFYYLYLGTGPEAGLYYLGTGPEA

Epitope source
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Spike
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Nucleoprotein
Membrane protein
Membrane protein
Membrane protein
Membrane protein
Membrane protein
Membrane protein
ORF 3a
ORF 3a
ORF 3a
ORF 3a
ORF 7a
ORF 7a
ORF 7aNucleoprotein
Nucleoprotein
Spike
Nucleoprotein
Nucleoprotein
Nucleoprotein