Instruction manual［11th Edition］

1-18-2 Hakusan, Midori-ku, Yokohama, Kanagawa
226-0006, JAPAN E-mail:eng@kyoritsu-lab.co.jpⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp.

4032

Table of Contents
1.… Introduction…………………………………………………………………………

4

2. Precautions for Use of Reagent… ……………………………………………

4

3. Precautions for Handling of Cell… ……………………………………………

4

4. Precautions for Measurement… ………………………………………………

5

5. Basic Measurement Operation Procedures… ………………………………

6

6. List of Analytes and List of Reagents…………………………………………

8

7. Normal measurement method… ………………………………………………

10

8. Measurement Methods… ………………………………………………………

14

Al			

Aluminum………………………………………………………………………

14

As			

Arsenic… ……………………………………………………………………………

15

As-D		

Arsenic（Low Range）… …………………………………………………………

17

B-C		

Boron（High Range）…………………………………………………………

18

B			

Boron… ………………………………………………………………………

19

Cd			

Cadmium………………………………………………………………………

20

Cl-500Chloride（High Range）………………………………………………………

21

Cl			

Chloride… ……………………………………………………………………

22

ClO-C		

Residual Chlorine（High Range）… ………………………………………

23

ClO-DPD

Residual Chlorine（Free）……………………………………………………

24

T-ClO		

Total Residual Chlorine… …………………………………………………

25

ClO2		

Chlorine Dioxide………………………………………………………………

26

NaClO2

Sodium Chlorite………………………………………………………………

27

NaClO2-D

Sodium Chlorite（Low Range）… …………………………………………

29

CN-2		

Free Cyanide… ………………………………………………………………

30

CNT		

Total Cyanide…………………………………………………………………

31

T

CN -D		

Total Cyanide（Low Range）… ……………………………………………

32

COD		

COD with KMnO4… …………………………………………………………

33

Color		

Color……………………………………………………………………………

34

Cr6+		

Chromium（Hexavalent）… …………………………………………………

35

6+

Cr -D		

Chromium（Hexavalent）（Low Range）……………………………………

36

CrT			

Total Chromium………………………………………………………………

37

Cu			

Copper…………………………………………………………………………

38

Cu-M-2

Copper（DDTC）………………………………………………………………

39

DET		

Anionic Surfactants…………………………………………………………

40

F			

Fluoride（Free）… ……………………………………………………………

41

Fe			

Iron… …………………………………………………………………………

42

Fe-D		

Iron（Low Range）… …………………………………………………………

43

Fe2+		

Iron（Divalent）… ……………………………………………………………

44

2+

Fe -D		

Iron（Divalent）（Low Range）… ……………………………………………

45

Fe3+		

Iron（Trivalent）… ……………………………………………………………

46

FOR		

Formaldehyde…………………………………………………………………

47

GLU		

Glucose… ……………………………………………………………………

48

H2O2-C

Hydrogen Peroxide（High Range）…………………………………………

49

H2O2		

Hydrogen Peroxide… ………………………………………………………

50

HYD		

Hydrazine………………………………………………………………………

51

KMnO4

Potassium Permanganate Consumption…………………………………

52

MAL		

M-Alkalinity……………………………………………………………………

53

PAL		

P-Alkalinity… …………………………………………………………………

54

Mn			

Manganese……………………………………………………………………

55

Mo			

Molybdenum… ………………………………………………………………

56

Ni-D		

Nickel（DPM）…………………………………………………………………

57

－ 2 －

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NH4		

Ammonium… …………………………………………………………………

58

NH4-N		

Ammonium-Nitrogen…………………………………………………………

59

NH4-D		

Ammonium（Low Range）……………………………………………………

60

NH4-N-DAmmonium-Nitrogen（Low Range）… ……………………………………

61

NO2-C		

Nitrite（High Range）…………………………………………………………

62

NO2-N-C

Nitrite-Nitrogen（High Range）… …………………………………………

63

NO2		

Nitrite… ………………………………………………………………………

64

NO2-N		

Nitrite-Nitrogen………………………………………………………………

65

NO3-C

Nitrate (High Range)… ……………………………………………………

66

NO3-C_1

ー2

Nitrate（High Range）（NO ≤1mg/L）……………………………………

67

NO3-C_2

Nitrate（High Range）（High Range）（NO2ー≤10mg/L）

68

NO3-N-C

Nitrate-Nitrogen (High Range)……………………………………………

69

NO3-N-C1

Nitrate-Nitrogen（High Range）（NO2ー-N ≤0.3mg/L）… ………………

70

NO3-N-C2

Nitrate-Nitrogen（High Range）（NO -N ≤3mg/L）… …………………

71

NO3

Nitrate…………………………………………………………………………

72

ー
2

NO3_1		

Nitrate（NO = 0mg/L）… …………………………………………………

73

NO3_2		

Nitrate（NO2ー≤0.2mg/L）……………………………………………………

74

ー
2

NO3_3		

Nitrate（NO ≤5mg/L）………………………………………………………

75

NO3-N

Nitrate-Nitrogen… …………………………………………………………

76

NO3-N_1

		

ー
2

ー
2

77

ー
2

Nitrate-Nitrogen（NO -N=0mg/L）… ……………………………………

NO3-N_2

Nitrate-Nitrogen（NO -N ≤0.06mg/L）… ………………………………

78

NO3-N_3

Nitrate-Nitrogen（NO2ー-N ≤1.5mg/L）… …………………………………

79

OIL-M		

Mineral Oil in Water… ……………………………………………………………

80

OIL-V		

Vegitable Oil in Water… …………………………………………………………

81

OIL-S		

Oil in Soil………………………………………………………………………………

82

Pb-SPK

Lead（SPK）… ………………………………………………………………………

83

PhenolPhenol（Reagent Model： WAK-PNL）… …………………………………

84

Phenol-2

Phenol（Reagent Model： WAK-PNL-2）… ………………………………

85

PO4-C		

Phosphate（High Range）… ………………………………………………

86

PO4-P-C

Phosphate-Phosphorus（High Range）……………………………………

87

PO4		

Phosphate… …………………………………………………………………

88

PO4-P		

Phosphate-Phosphorus… …………………………………………………

89

PO4-D		

Phosphate（Low Range）……………………………………………………

90

PO4-P-D

Phosphate-Phosphorus（Low Range）……………………………………

91

S			

Sulfide（Hydrogen sulfide）…………………………………………………

92

SiO2		

Silica……………………………………………………………………………

93

SiO2-D

Silica（Low Range）… ………………………………………………………

94

SO4		

Sulfate…………………………………………………………………………

95

TH			

Total Hardness… ……………………………………………………………

96

TN-2		

Total Nitrogen… ……………………………………………………………

97

TP-2		

Total Phosphorus……………………………………………………………

98

Turbid-F

Turbidity（Formazine）… ……………………………………………………

99

Turbid-P

Turbidity（Polystyrene）… …………………………………………………

100

Zn-D		

Zinc（Low Range）……………………………………………………………

101■ T his entire instruction manual is protected by copyright. No part of this instruction manual may be
reproduced in any form without permission.

		■ The contents of this instruction manual are subject to change without notice.

		■ The specifications of this product are subject to change without notice.

		■ Changes and additions to analyte will be reported via our website or other media as needed. 		
https://kyoritsu-lab.co.jp/english/index.html－ 3 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

1. Instruction
Thank you for purchasing DIGITALPACKTEST·MULTI SP (model: DPM-MTSP).
This product is incorporated with calibration curve data that have been obtained by using PACKTEST or otherreagents and enables simple measurement of the concentration of analyte in a sample.
Read this instruction manual before use to thoroughly understand the functions of this product for proper operation.

2. Precautions for Use of Reagent
Use reagents made by KYORITSU CHEMICAL-CHECK Lab., Corp. As the reagent varies depending on the analyte, referto the instructions. Use a reagent after reading the instructions, the GHS symbols, and SDS. For precautions and therelevant first aid method on reagent prepared by the user, refer to the SDS supplied from the manufacturer.
＜ Safety measurement ＞● Thoroughly clean the hands before and after measurement. Do not inhale or contact the reagent.
● Wear PPE such as protective gloves, protective goggles, and mask as much as possible.
● Do not leak reagent or waste liquid into the surrounding environment.

＜ First aid ＞
If reagent or solution enters your eye→

immediately wash your eye with water for 15 minutes or longer.

		Immediately consult an ophthalmologist even if you do not experience any pain or abnormal symptoms.
If reagent or solution contacts your skin or clothing→

immediately rinse the skin or clothing with water.

If reagent or solution enters your mouth→

immediately rinse your mouth with water.

If you have swallowed reagent or solution or you experience any abnormal symptoms after receiving the above
treatment, immediately consult a doctor.
For details, refer to the instructions for the reagent or the SDS.
＜ Storage ＞ Store reagent in the dry and dark place at room temperature beyond the reach of children.
＜ Disposal ＞ Appropriately dispose of reagent according to the relevant laws and regulations.
＜ Others ＞

Before using a reagent, check its expiration date. Measurement using a reagent beyond its expiration period is invalid.

3. Precautions for Handling of Cell
Use PACKTEST Square Cup as a cell. For some analytes, Round Cell is used asa reaction container.
1.Use "PACKTEST Square Cup" (model: WAK-CC10) as the Cell.
2.Use the same Cell in steps from zero adjustment to measurement.
3.The side faces of the Cell serve as an optical path. Do not hold the faces
where light passes with your hand.
4.If the water temperature is significantly lower than the ambient temperature, dew may be condensed on the Cell tocause turbid and abnormal measurement value.
5.When setting the Cell in the cell box, cleanly wipe its surface so as not to leave water droplets or finger prints, andthen gently put it the cell box.
6.When setting the Cell in the cell box, remove the cap of the cell to prevent the leak.
7.After measurement, take out the Cell or Round Cell, clean it with pure water, because reagent or other substances
remaining in it may cause a measurement error in the next measurement. If pure water is not available, thoroughly
rinse it with tap water, and then clean it with the sample before conducting the next measurement.
8.As a flaw or dirt on the Cell causes a measurement error, replace the Cell with a new one as necessary.
9.The material of the Cell and that of Round Cell are as follows. Dispose of them according to the relevant instructionof the local government.
Sort of cell
PACKTEST Square Cup
Round Cell

Material of cell
polystyrene
glass

Material of cap
polyethylene
polypropylene（PP）

10. Values displayed when the Cell is not set in the cell box are i nvalid.
－ 4 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

4. Precautions for Measurement
1.As a basic measurement method, put the sample in PACKTEST Square Cup, cancel the sample color as of before
measurement by pressing【Zero】, and then press【Start】after sucking the sample into the tube or adding thespecified reagent. Have the reagent react with the sample by lightly shaking to produce a solution, and put the Cellto which the solution has been returned in the cell box within the reaction time. Then, the result is automaticallydisplayed when the reaction time ends.
2.As the measurement reagent, the PACKTEST, Reagent Set for Water Analyzer or an analysis set of varying type isused depending on the analyte. When performing measurement by using PACKTEST or an analysis set of varying type,also carefully read the instruction for the reagent. Note that the reaction time, measurement range, and influenceof coexistent substance differ between the case where the Standard Color and the color development are visuallycompared and the case where measurement is conducted by using this measurement device. Also, depending on theanalyte, a special pretreatment method, a tool (sold separately) or a reagent (sold separately or separately procured by
the customer) may be required. Carefully read "8. Measurement Methods".
3.Adjust the pH of the sample within the pH range specified for the analyte. As the reagent contains pH buffer, pH
adjustment of a sample near neutral is unnecessary, but be sure to neutralize a strong acid or strong alkali sample,
in particular an acidified or alkalized sample.
4.If the sample is excessively turbid or colored, zero adjustment may not be possible. Perform filtration, dilution, andthe like as necessary.
5.The reagent may not be completely dissolved. This does not affect color development, but floating of the reagent
in the solution or attachment of the reagent to the wall surface of the Cell causes a positive measurement error.
Therefore, place the Cell during reaction time. If remaining undissolved reagent or bubbles have attached to thewall surface of the Cell, remove them as much as possible by, for example, snapping the Cell with your finger. Be
especially careful if such attachment occurs on the low concentration because the error becomes larger.
6.Set the sample temperature to 15 to 30 ℃ for measurement. Note that for some analytes, temperature correction
coefficients are listed. As the calibration curve and various data for DIGITALPACKTEST･MULTI SP have beencreated at 20℃ , measurement at 20℃ obtains better measurement data. If the sample temperature greatly deviatesfrom the target temperature, perform measurement in any of the following methods.
		 ⑴ Perform measurement after setting the sample temperature to 20℃ by using a temperature controlled bath or the like.
		 ⑵ If a temperature correction coefficient is listed in the "CAUTION" column, multiply the measurement value by the coefficientfor the temperature nearest to the sample temperature during measurement to obtain an approximate value.
7.A result can be estimated by the shading of the color development of the solution. If a measurement value could beobtained even when no color development occurred, there is a possibility of turbid due to change of pH caused by
the color development reagent. Also, note that depending on the analyte, the color development may become pale
if its concentration is excessively high or a measurement value may be obtained even from a sample exceeding themeasurement range.
8.If a measurement value is out of the measurement range, "UNDER" or "OVER" is displayed.
9.When the concentration of the analyte is considered to be high or when the result is above the measurement range, dilutethe sample with pure water so that the result falls within the measurement range.
10.	There are data on the influence of coexistent substance for respective items. If coexistent substance is considered
to exert influence, perform measurement after an appropriate pretreatment according to JIS K 0102 or otherstandard. Depending on the type of coexistent substance, measurement may be impossible.
11.	The result obtained by Photometry mode using PACKTEST etc. as measuring reagent is not certificated value.
For first time use or when doubt about the result, you may need to check the correlation with official method.
－ 5 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

5. Basic Measurement Operation Procedures
The basic measurement operation procedures are explained below.

Fill the Cell with 1.5 mL of sample (up to line).
Place the Cell on a flat surface.
Move your eyes to the same height as the liquid level, and put the sample up to the point where
the lower end of the liquid surface (meniscus) aligns with the line on the Cell.
Fill the Round Cell with 25 mL of sample (up to white line).
Place the Round Cell on a flat surface.
Move your eyes to the same height as the liquid level, and put the sample up to the point where
the lower end of the liquid surface (meniscus) aligns with the line on the Round Cell.

=HUR

Press the【 Zero】button.

6WDUW

Press the【 Start】button.

Cancel the color of the sample as of before measurement.

Count down of the time set on "Reaction time" starts.
As soon as the reaction time ends, the absorbance is measured and the result is displayed.

Lightly shake the tube 5 to 6 times.

5-6

Amount of the sample into the tube of PACKTEST, slowly overturn the tube so as not to spill
the sample in it.

Shake the tube for about 10 times.
After sucking the whole amount of the sample into the tube of PACKTEST, slowly overturn the
tube so as not to spill the sample in it.

Stir the sample for XX times in XX minutes.
After sucking the whole amount of sample into the tube of PACKTEST, overturn the tube at the○ times for ○ minutes

speed of about one reciprocation per second so as not to spill the sample in it.

Return the sample to the Cell when the time count down reaches under XX minutes.
As bubbles are generated during reaction and they are likely to attach to the wall surface of theCell, return the solution from the tube of PACKTEST to the Cell XX minutes before the end ofreaction time.

－ 6 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Snap the Cell with your finger to remove the bubbles on the wall surface.
If bubbles remain after returning the solution to the Cell, snap the Cell with your finger to remove
the bubbles on the wall surface.
Wait for XX minutes.
The waiting time is displayed inside the mark.
Wait after placing the Cell or the Round Cell on a flat surface.
Or place the tube after shaking.
Stir 5 to 6 times.
Hold the top part of the Round Cell and stir the solution in a circular motion.

Shake 5 to 6 times.
Vertically shake the Round Cell 5 to 6 times so that the liquid in it is well mixed.

Immediately shake strongly for approx. 10 seconds.
Attach the cap immediately after adding the reagent, and shake the Round Cell strongly for approx.
10 seconds at the speed of about 2 reciprocations per second.

－ 7 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

6. List of Analytes and List of Reagents
As a major reagent, PACKTEST (sample amount: 1.5 mL, model: WAK -), Reagent Set for Water Analyzer (sample amount:
25 mL, model: LR -), analysis set of varying types (model: WA -), and DPR reagent for water analyzer (model: DPR -) are used.
AnalyteRange（mg/L） Reaction Time Reagent Model Note / Necessary instruments

Al

Aluminum

0.05 〜 0.40

5 min

LR-Al

As

Arsenic0.20 〜 3.00

［30 min］

DPR-AsAs-D

Arsenic（Low Range）

0.009 〜 0.200［12 min］

SPK-As（D）

B-C

Boron（High Range）

5.0 〜 80.0

12 minWAK-B（C）

B

Boron

0.50 〜 6.00

40 min

WAK-B

Cd

Cadmium

0.003 〜 0.035［5 min］

SPK-CdCl-500Chloride（High Range）

20 〜 500

3 min

DPR-ClCl

Chloride

2.0 〜 50.0

3 min

DPR-ClClO-C

Residual Chlorine（High Range）

2 〜 500

1 min

WAK-ClO（C）

ClO-DPD

Residual Chlorine（Free）

0.05 〜 3.00

1 min

WAK-ClO･DP

T-ClO

Total Residual Chlorine

0.05 〜 3.00

2 min

WAK-T･ClO

ClO2

Chlorine Dioxide

0.20 〜 6.00

30 sec

WAK-ClO 2

NaClO2

Sodium Chlorite

2 〜 500

1 min

WAK-NaClO 2

NaClO2-D Sodium Chlorite（Low Range）

0.10 〜 2.00

1 min

WAK-NaClO2（D）

CN-2

Free Cyanide

0.01 〜 1.00

10 minCN T

Total Cyanide

0.1 〜 3.0

［30 min］

LR-CN T

Water Analysis Set:
Total Cyanide

CN T-D

Total Cyanide（Low Range）

0.005 〜 0.150［50 min］

LR-CN-B

CN T-RA

COD

COD with KMnO4

2.0 〜 10.0

10 min

Color

Color

50 〜 1000deg.

0 min

−

0.05 〜 1.50

2 min

WAK-Cr6

0.003 〜 0.100［10 min］

DPR-Cr6 D

Cr

6+Chromium（Hexavalent）

WAK-CN-2

LR-CODｰB-2++

Cr6 +-D

Chromium（Hexavalent）（Low Range）

Cr T

Total Chromium

0.05 〜 1.50

［12 min］

WAK-Cr6

Cu

Copper

0.10 〜 5.00

1 min

WAK-CuCu-M-2

Copper（DDTC）

0.5 〜 10.0

2 min

WAK-CuM-2

DET

Anionic Surfactants

0.05 〜 1.20

［3 min］

WA-DETF

Fluoride（Free）

0.40 〜 1.50

10 min

WAK-F

Fe

Iron

0.10 〜 5.00

3 min

WAK-FeFe-D

Iron（Low Range）

0.05 〜 2.00

3 min

WAK-Fe（D）

Fe2+

Iron（Divalent）

0.10 〜 5.00

3 min

WAK-Fe2

Fe2+-DIron（Divalent）（Low Range）

0.05 〜 2.00

3 min

WAK-Fe2（D）

Fe3 +Iron（Trivalent）

1.0 〜 50.0

1 min

WAK-Fe3

FOR

Formaldehyde

0.20 〜 1.00

5 min

WAK-FORGLU

Glucose0.5 〜 20.0

12 minWAK-GLU

H2O2-C

Hydrogen Peroxide（High Range）

1 〜 200

1 min

WAK-H 2O2（C）

H 2O 2

Hydrogen Peroxide

0.10 〜 2.50

2 min

WAK-H 2O2

HYD

Hydrazine

0.03 〜 1.00

20 minWAK-HYD

KMnO 4

Potassium Permanganate Consumption

2.0 〜 10.0

10 minLR-CODｰB-2

MAL

M-Alkalinity

20 〜 80

2 min

WAK-MALPAL

P-Alkalinity

100 〜 600

1 min

WAK-PAL

Mn

Manganese

0.5 〜 20.0

3 min

WAK-Mn

－ 8 －

+

Cr-RA

+
+

+

Use the same Reagent as CODⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

Analyte

Range（mg/L） Reaction Time Reagent Model Note / Necessary instruments

Mo

Molybdenum

5 〜 150

2 min

WAK-MoNi-D

Nickel（DPM）

0.3 〜 10.0

2 min

WAK-Ni（D）

NH 4

Ammonium0.20 〜 5.00

10 minWAK-NH 4-4

NH 4-N

Ammonium-Nitrogen

0.20 〜 4.00

10 minWAK-NH 4-4

NH 4-D

Ammonium（Low Range）

0.05 〜 2.00

［40 min］

LR-NH 4-A-2

WA-NH 4-DR

NH 4-N-D

Ammonium-Nitrogen（Low Range）

0.05 〜 1.50

［40 min］

LR-NH 4-A-2

WA-NH 4-DR

NO2-C

Nitrite（High Range）

3 〜 100

5 min

WAK-NO 2（C）

NO2-N-C

Nitrite-Nitrogen（High Range）

1.0 〜 30.0

5 min

WAK-NO 2（C）

NO2

Nitrite0.02 〜 1.00

3 min

WAK-NO 2

NO2-N

Nitrite-Nitrogen

0.010 〜 0.300

3 min

WAK-NO 2-

200 〜 2000

5 min

WAK-NO3（C）-

200 〜 2000

［10 min］

WAK-NO3（C）

45 〜 450

5 min

WAK-NO3（C）（NO2 -N≤3mg/L）
NO3-N-C2 Nitrate-Nitrogen（High Range）

45 〜 450［10 min］

WAK-NO3（C）

NO3̲1

-

Nitrate（NO2 = 0 mg/L）

1.0 〜 25.0

5 min

WAK-NO3

NO3̲2-

Nitrate（NO2 ≤0.2 mg/L）

1.0 〜 25.0

［8 min］

WAK-NO3

WAK-NO2

NO3̲3

Nitrate（NO2-≤5mg/L）

1.0 〜 25.0

［10 min］

WAK-NO3

NO3-RA, Heater

NO3-N̲1

Nitrate-Nitrogen（NO2- -N=0mg/L）

NO3-C̲1

Nitrate（High Range）（NO2 ≤ 1 mg/L）（NO2 ≤10mg/L）
NO3-C̲2 Nitrate（High Range）-（NO2 -N≤0.3mg/L）
NO3-N-C1 Nitrate-Nitrogen（High Range）-

NO3-RA, Heater

NO3-RA, Heater

0.20 〜 5.80

5 min

WAK-NO3-

0.20 〜 5.80

［8 min］

WAK-NO3

WAK-NO2-

NO3-N̲3 Nitrate-Nitrogen（NO2 -N≤1.5mg/L）

0.20 〜 5.80

［10 min］

WAK-NO3

NO3-RA, Heater

OIL-M

Mineral Oil in Water

5.0 〜 60.0

［15 min］

WA-OIL-R

OIL-V

Vegetable Oil in Water

5.0 〜 60.0

［15 min］

WA-OIL-R

OIL-S

Oil in Soil

400 〜 5000mg/kg［10 min］

SOA-OIL-RR

Pb-SPK

Lead（SPK）

0.03 〜 0.50

［12 min］

SPK-Pb

PhenolPhenol0.20 〜 5.00

8 min

WAK-PNL

Phenol-2 Phenol

0.20 〜 5.00

3 min

WAK-PNL-2

PO 4-C

Phosphate（High Range）

2.0 〜 50.0

3 min

（C）
WAK-PO 4

PO 4-P-C

Phosphate-Phosphorus（High Range）

0.7 〜 15.0

3 min

WAK-PO 4（C）

PO 4

Phosphate

0.10 〜 5.00

3 min

WAK-PO 4

PO 4-P

Phosphate-Phosphorus

0.03 〜 1.50

3 min

WAK-PO 4

PO 4-D

Phosphate（Low Range）

0.10 〜 3.00

5 min

WAK-PO 4（D）

PO 4-P-D

Phosphate-Phosphorus（Low Range）

0.03 〜 1.00

5 min

WAK-PO 4（D）

S

Sulfide（Hydrogen sulfide）

0.05 〜 0.80

3 min

WAK-S

SiO2

Silica3.0 〜 60.0

8.5 min

WAK-SiO 2

SiO2-D

Silica（Low Range）

0.30 〜 7.00

8.5 min

WAK-SiO 2
（D）

SO 4

Sulfate5 〜 100

3 min

DPR-SO 4

TH

Total Hardness

10 〜 150

1 min

WAK-TH

TN-2

Total Nitrogen

0.5 〜 7.0

［60 min］

TNP-N-R

TP-2

Total Phosphorus

0.10 〜 2.00

［60 min］

TNP-P-R

Turbid-F

Turbidity（Formazine）

10 〜 400deg.

0 min

−

Turbid-P

Turbidity（Polystyrene）

10 〜 100deg.

0 min

−

Zn-D

Zinc（Low Range）

6 min

WAK-Zn（D）

NO3-N̲2 Nitrate-Nitrogen（NO2 -N≤0.06mg/L）

0.02 〜 0.40

Water Analysis Reagent Set: Oil（resolution : 0.5 mg/L）
Water Analysis Reagent Set: Oil（resolution : 0.5 mg/L）
Soil Screening Refill Reagent Set: Oil（resolution : 100 mg/kg）

Released from June, 2023.

Mini Autoclave Set
HeaterMini Autoclave Set
HeaterReaction Time：［ ］are estimated reaction time including pretreatment procedure. Specifications subject to change without notice.
－ 9 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

7. Normal measurement method
Photometry(1 item)
Selecting analyte
1. Press【Photometry】on [Main window].

Name of reagent used2.	[Analyte Selection Window] is displayed.
	Pressing【▼】brings you to the next page.

Analyte nameInput
button	Pressing【▲】brings you to the previous page.
3. Press the desired analyte symbol.
4.	The analyte name and the name of reagent used are displayed, and the

Selected
analytesymbolanalyte symbol is displayed in an input button.
Jumps tonext window.

5.	Pressing【OK】brings you to [Photometry Window].

AnalytesymbolCanceling analyte
1.	Press an input button on which the desired analyte symbol is displayed.
2.	Pressing【Clear】clears the contents of the input button and the analyte is canceled.

Performing zero adjustment
1. Fill the Cell with the sample.
2. Put the Cell in the cell box.
3. Press【Zero】.
4.	When the zero adjustment finishes, the color of【Zero】is inverted.
"0" is displayed in the result display area.

Reaction time:
Measurement range

Counts down during
measurement.

- Zero adjustment can be performed any number of times.
- During measurement,【Zero】is disabled.
- After measurement, the color inversion of【Zero】returns to theoriginal state.
ResultResult (Manual)

Performing measurement
1.	After zero adjustment, mix the sample with the reagent. At the same time, press【Start】.
2.	The color of【Start】is inverted and countdown of reaction time starts.
3.	Pour the solution to the Cell and put the Cell in the cell box.
4.	After the reaction time has elapsed, the result is displayed.
5.	The result will be saved automatically. (When a memory card is inserted)
6.	The result will be printed out automatically. (When a printer is connected)- Before zero adjustment,【Start】is disabled.
- At 30 seconds before the reaction time ends, the buzzer issues a bleep as a reminder to set the cell.
- After measurement, the color inversion of【Start】returns to the original color.
- After measurement,【Start】is disabled. Perform the steps from zero adjustment.
- During measurement, it is not possible to turn off the power supply.
- Even when a memory card is inserted, automatic saving may not be executed if it is locked or the data in it
have reached the upper limit.

－ 10 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Performing manual measurement
1. Press【Read】.
2.	A result is displayed.
3.	The result will be saved automatically. (When a memory card is inserted)
4.	The result will be printed out automatically. (When a printer is connected)- Manual measurement is enabled after zero adjustment, during measurement and after measurement
finished.
- Even when a memory card is inserted, automatic saving may not be executed if it is locked or the data in it
have reached the upper limit.
Canceling measurement
1. Press【Cancel】.
2.	The instrument state returns to the state as of before zero adjustment.
- After zero adjustment,【Cancel】is enabled.
- During measurement, measurement is canceled. The stopped measurement cannot be resumed.
- If【Cancel】is pressed during and after measurement, the result are deleted from the window.
- To turn off the power during measurement, first press【Cancel】.
Viewing data
1. Press【Data】.
2. [Photometry Data Window] is displayed.
3.	It is possible to view the past results stored in the memory card.

Jumps to next window.

－ 11 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Photometry (parallel measurement of four samples)
Measurement example 1 (multiple analytes)
Measurement for multiple analytes or measurement ofmultiple samples for the same analyte can be performedin parallel.
It is possible to set items 1 to 4.

Measurement example 2 (multiple samples for the same analyte)

Selecting analyte
1. Press【Photometry】on [Main Window].

Name of reagent used2.	[Analyte Selection Window] is displayed.

Analyte name

3. Press an input button.
4.	Press the desired analyte symbol.

Input button

5.	The analyte name and the name of reagent used are displayed, and the analytesymbol is displayed in an input button. (Selection of item 1 has finished.)

Selected
analyte symbol

6.	Press a different input button from that in Step 3. The cursor moves.
7.	If you press a analyte symbol, a analyte name and the name of reagent used
are displayed, and the corresponding analyte symbol is displayed in an input

Jumps tonext window.

Analyte symbol

button. (Selection of item 2 has finished.)
8.	To set item 3 and item 4, repeat the steps 6 to 7.
9.	Pressing【OK】brings you to [Photometry Window].
Canceling analyte
1.	Press an input button on which the desired analyte symbol is displayed.
2.	Pressing【Clear】clears the contents of the input button and the analyte is canceled.
[Item1]  Performing zero adjustment and measurement
1.	Fill the Cell with the sample.
2.	Put the Cell in the cell box.
3.	Press【Zero】.
4.	When the zero adjustment finishes, the color of【Zero】is inverted. "0" is displayed in the
result display area for item 1.
5.	After zero adjustment, mix the sample with the reagent. At the same time, press【Start】.
6.	The color of【Start】is inverted and countdown of reaction time starts.

Analyte symbol

Result

7. Pour the solution to the Cell.
- Inversion of【Zero】and【Start】indicates the state of the selected analyte.

－ 12 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

[Item2]  Performing zero adjustment and measurement
1.	Fill the Cell with the sample.
2.	Press the desired analyte symbol for item 2.
3.	The inverted state of【Zero】and【Start】returns to the original state.
4.	Put the Cell in the cell box.
5.	Press【Zero】.
6.	Mix the sample with the reagent. At the same time, press【Start】.
7. Pour the solution to the Cell.
- Repeat the same steps for item 3 and item 4.
Finishing measurement
1.	A flag is displayed on the analyte whose reaction time finishes most early.
2.	Put the Cell for the analyte indicated with the flag in the cell box.
3.	After the reaction time has elapsed, the result is displayed in black in the result display
area.
4.	The result will be saved automatically.
5. 	The result will be printed out automatically.
Flag

6.	A flag is displayed on the analyte whose reaction time finishes next.
7.	Repeat steps 2 to 6.
Performing manual measurement
1.	Press a analyte symbol for which you want to perform manual measurement.
2.	Press【Read】.
is displayed at the right end.
*
4.	The result will be saved automatically.
3.	A result is displayed in red.

5.	The result will be printed out automatically.
Canceling measurement
1.	Press a analyte symbol whose measurement you want to cancel.
2.	Press【Cancel】.
3.	Only for the selected analyte, the state returns to that of before zero adjustment.
- Only for the selected analyte,【Cancel】is enabled.
- After zero adjustment,【Cancel】is enabled.
- During measurement, measurement is stopped. The stopped measurement cannot be resumed.
- If【Cancel】is pressed during and after measurement, the result are deleted from the window.
- To turn off the power during measurement, first cancel the ongoing measurement.
Viewing data
1.	Press【Data】.
2.	[Photometry Data Window] is displayed.
3.	It is possible to view the past results stored in the memory card.
- During countdown of reaction time,【Data】is disabled.

－ 13 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

8. Measurement Methods
Al

Aluminum
Cell ： PACKTEST Square Cup
Wavelength ： 533 nm, 560 nm

Color development： Yellow → Orange → Red
Method： ECR
Range： 0.05 ー 0.40 mg/L（ppm）
Reagent： LR-Al No.24 R-1（Liquid）, R-2（Pack）
Reaction time ： 5 minutes after R-2 reagent is added.

Procedure
1. Press【Al】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line) and fill the Round
Cell with the sample for 25 mL (up to white line).（Fig.1）

=HUR
Fig.1

Fig.2

4. Put the Cell in the cell box and press【Zero】. Discard the sample in
the Cell.（Fig.2）
5. Add 2 mL of R-1 reagent into the Round Cell using the supplied
pipette, tightly attach the cap, and shake the Round Cell 5 to 6
times.（Fig.3）
6. Add the R-2 reagent, press【Start】, tightly attach the cap, andintensely shake the Round Cell for approx. 10 seconds.（Fig.4）

5

5

6WDUW
Fig.3

Fig.4

7. Within 5 minutes, pour the solution in the Round Cell for 1.5 mL into
the Cell that has gone through zero adjustment (up to line) and put
the Cell in the cell box.（Fig.5）
8. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

Caution
1. In this method, the concentration of ionized aluminum (Al3+) in the sample is measured. If result of Aluminum concentration
including suspension and precipitate is required, dissolve Aluminum in advance and then perform measurement.
2. The dissolved state of aluminum greatly varies depending on the pH of the sample, and aluminum could exist in the form ofsuspended solid or precipitate. Perform measurement after pretreatment according to the measurement purpose.
3. The optimum pH during color development is 6.If this pH cannot be achieved, neutralize the sample as necessary.
		 A sample with a small buffering capacity can be measured even if its pH is around 2.
4. Perform measurement with the sample temperature set to 15 to 30℃ .
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing it withthe official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:≤ 100mg/L,:	B（Ⅲ）, Ca2+ , Cl － , I － , K+ , Mg2+ , Na+ , NH4+ , NO2 － , NO3 － ,SO 42－ , Anionic Surfactant , Phenol , Residual Chlorine≤ 10mg/L,: PO 43 －< 1mg/L,: F －Heavy metal ions:

It is not possible to measure seawater.

－ 14 －≤ 10mg/ L ,:	Ba2+ , CN － , Co2+ , Cu2+ , Fe2+ , Fe3+ , Mn2+ , Mo（Ⅵ）, Ni2+ , Zn2+
≤ 1mg/L,: Cr 3+< 1mg/L,: Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

As

Arsenic

Cell ： PACKTEST Square Cup
Color development： None → Light blue → Blue
Wavelength ： 650 nm
Method： Molybdenum BlueRange
： 0.20 ー 3.00 mg/L（ppm）
Reagent： DPR-As R-1（Liquid）, R-2（Liquid）, R-3（Liquid）, R-4（Liquid）, Tube
Reaction time ： 5 minutes after drawing sample into the tube.

FeaturesIn the Molybdenum-blue absorptiometry method, which is the color development principle of this product, the colors of arsenate
ions (As( Ⅴ )) and phosphate ions (PO43－ ) develop in the same manner.
If arsenate ions, arsenite ions (As( Ⅲ )) and phosphate ions exist in the sample, first reduce the arsenate ions into arsenite
ions and develop the color of phosphate ions only, and use this color as a reference. (the upper row of "Measurement chart"
below)
Next, oxidize the arsenite ions into arsenate ions and develop their color together with the original arsenate ions andphosphate ions. (The lower row of "Measurement chart" below)
The total value of arsenate ions and arsenite ions is obtained through subtraction between these two color developments, and it
is converted into an arsenic value.

CautionIf phosphate ions coexist at a concentration of 1 mg/L or higher, it is not possible to measure the concentration of arsenate
ions.

Measurement chart
6DPSOH

5UHDJHQW

5UHDJHQW

5UHDJHQW

7XEH‫ق‬3RZGHU‫ك‬=HUR!

6DPSOH

5UHDJHQW

5UHDJHQW

5UHDJHQW

3RXURXW5LQVH

7XEH‫ق‬3RZGHU‫ك‬6WDUW!

5HVXOW

ProcedureFirst, develop the color of phosphate ions only for the purpose of zeroadjustment.
1. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）
2. Add one droplet of R-1 reagent, attach the cap, and shake the Cell 2
to 3 times. （Fig.2）
3. Add two droplets of R-2 reagent, attach the cap, shake the Cell 2
to 3 times, and leave for 10 minutes. （Fig.3）
4. Add four droplets of R-4 reagent, attach the cap, and shake the Cell
2 to 3 times. （Fig.4）

Fig.1

Fig.2

5

Fig.3

Fig.4

5. Suck the whole amount of the sample in the Cell into the tube andthen lightly shake the tube 5 to 6 times. （Fig.5）
6. Return the solution to the Cell and wait for 5 minutes. （Fig.6）
Fig.5－ 15 －Fig.6Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

7. Press【As】.
8. Press【OK】to switch to the photometry window.
9. Put the Cell in which only the color of phosphate has been developedin the cell box, and press【Zero】.（Fig.7）
10.Take the Cell out of the cell box, empty it, and clean the Cell and cap
with pure water.（Fig.8）

7KHVDPH&HOO=HUR

3XUHZDWHU

Fig.7

Fig.8

Fig.9

Next, develop the colors of phosphate ions, arsenate ions and arsenite
ions, and obtain a result.
5

5

11.Fill the Cell in Step 10 with the sample for 1.5 mL (up to line).（Fig.9）
12.Add one droplet of R-1 reagent and one droplet of R-3 reagent,attach the cap, and shake the Cell 2 to 3 times.（Fig.10）

Fig.10

Fig.1113.Add four droplets of R-4 reagent, attach the cap, and shake the Cell
2 to 3 times.（Fig.11）
14.Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.12）

6WDUW
Fig.12

Fig.13

15.Lightly shake the tube in Step 14 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in thecell box.（Fig.13）
16.After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Caution
1. In this method, it is possible to measure the concentration of arsenate ions (As ( Ⅴ )) and arsenite ions (As ( Ⅲ )) in the sample, andnot possible to measure the concentration of arsenic in other forms.
2. The optimum pH during color development is 2. If the pH of the sample is not within the range from 2 to 9, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. It is not possible to perform measurement of a sample containing arsenate ions at a concentration of 10 mg/L or higher or asample in a highly oxidized state.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing it withthe official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Oxidizing substance or reductive substance may affect themeasurement.
It is not possible to measure seawater.

－ 16 －≤ 1000mg/L,:	B（Ⅲ）, Cl − , CN − , K+ , Na+ , NH 4+ , SO 42 － , Zn2+
≤ 500mg/L,:	Ca2+ , Mg2+ , NO3 −≤ 200mg/L,:	NO2 －≤ 100mg/L,:	Cr 3+ , Ni2+ , Silica≤ 50mg/L,:	Al3+ , Co2+ , F − , I － , Mn2+ , Mo（Ⅵ）, Phenol≤ 10mg/L,: Fe2+ , Fe3+
≤ 5mg/L,: Cr（Ⅵ）≤ 1mg/L,: PO 43 － , Residual Chlorine< 1mg/L,: Ba2+ , Cu2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

As-D

Arsenic（Low Range）

Cell ： PACKTEST Square Cup
Color development： None → Light blue → Blue
Wavelength ： 640 nm
Method： Separation with a syringe filter / Molybdenum BlueRange
： 0.009 ー 0.200 mg/L(ppm)
Reagent： SPK-As(D) K-1（Liquid）, K-2（Liquid）, K-3（Liquid）, K-4（Liquid）, Tube
Reaction time ： 5 minutes after drawing sample into the tube.
Preparation Procedure： Read the instruction for the reagent (SPK-As(D)).
0.5

Procedure

6\ULQJH.3

Strong Acid

1. Press【As-D】.

&RQQHFW HDFKSDUWVrUPO\WR
SUHYHQW IURPOHDNLQJ

2. Press【OK】to switch to the photometry window.

7UHDWHG)LOWHU.

3. Perform procedures of "Collection" in the instruction supplied with thereagent (SPK-As(D)).

=HUR

1R]]OH

Fig.1

Fig.2

Fig.3

4. Press the treated filter onto the syringe firmly, and connect thenozzle.（Fig.1）

1.0
mL
1.0
mL

5. Fill the Cell with K-3 Reagent for 1.5 mL (up to line).（Fig.2）

0.5.3

3UHVV
2XW

0.5

6. Put the Cell in the cell box and press【Zero】.（Fig.3）

.3

'UDZ
,Q

7. Add four droplets of K-4 Reagent to the Cell.（Fig.4）.

6PDOODPRXQW
RI VROXWLRQZLOOUHPDLQ

1.0mL

8. Draw the solution slowly from the Cell into the syringe, as much as
possible. Press out the solution slowly to the Cell.
		 Repeat this step twice.（Fig.5）

Fig.4

 WLPHV

Strong Acid

Fig.5

0.5
.3

9. Press out the solution completely to the Cell.（Fig.6）

3UHVV2XW&RPSOHWHO\

10.Suck the whole amount of the solution in the Cell into the tube andpress【Start】at the same time. （Fig.7）
11.Invert the tube lightly end to end for 30 times to mix with reagent.
（Fig.8）,I VROXWLRQ UHPDLQVLQVLGH WKH QR]]OHUHPRYHWKHV\ULQJHGUDZ LQ VRPH DLU
FRQQHFW EDFN DQG
SUHVVRXWWKHDLU
溶出液

6WDUW

Fig.6

Fig.7

Fig.8

Fig.9

12.Return the solution back into the Cell slowly, and set the Cell into thecell box. （Fig.9）
13.After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Caution
1. Refer to the instruction for the reagent (SPK-As(D)).
2. Perform measurement with the solution temperature set to 15 to 30℃ .

Influence of coexisting substance
Refer to the instruction for the reagent (SPK-As(D)).

－ 17 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

B-C

Boron (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 490 nm

Color development： Light yellow → Yellow
Method： Azomethine H
Range： 5.0 ー 80.0 mg/L（ppm）
Reagent： WAK-B（C） K-1（Liquid）, Tube
Reaction time ： 12 minutes after drawing sample into the tube.

Procedure
1. Press【B-C】.

.
P/
6DPSOHP/

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 0.2 mL by using the small pipette.
（Fig.1）
4. Add the K-1 reagent for 1.5 mL to the sample in the Cell by using thelarge pipette, attach the cap, and shake the Cell 2 to 3 times.（Fig.2）

Fig.1

Fig.2

5. Remove the cap of the Cell, set the Cell in the cell box and press【Zero】.（Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.4）
7. Shake the tube in Step 6 by overturning it to right and left for 60
times in 1 minute.（Fig.5）

=HUR

6WDUW

Fig.3

Fig.4

Fig.5

Fig.6

8. Return the solution in the tube to the Cell in a gentle manner, set it
again in the cell box.（Fig.6）
9. After 12 minutes have elapsed, the concentration will be automaticallydisplayed.

Caution
1. In this method, the concentration of ionized borate (borax) is measured and it is converted into a boron concentration value.
It is impossible to measure the concentration of fluoroborate (BF4－ ).
2. Use the small pipette for sample after thoroughly cleaning it with pure water or cleaning its inside with the sample.
3. The optimum pH during color development is 6. If the pH of the sample is not within the range from 5 to 9, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 15℃・・・×0.95
25℃・・・×1.20
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing it withthe official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement. (However, ordinary
seawater contains boron at 4 to 5 mg/L.)－ 18 －≤ 5000mg/L,:	As（Ⅲ）, Cl − , F − , I － , K+ , Na+ , NH 4+ , NO2 － , NO3 － ,PO 43 － , Phenol≤ 2500mg/L,:	Mg2+ , Mn2+
≤ 1000mg/L,: Ni2+ , SO 42 － , Zn2+
≤ 500mg/L,: Ba2+ , Ca2+
≤ 250mg/L,: Al3+ , Cr 3+ , Anionic Surfactant≤ 100mg/L,:	Cu2+≤ 50mg/L,: CN － , Cr（Ⅵ）, Residual Chlorine≤ 25mg/L,: Fe2+ , Sn2+
< 1mg/L,: Ag+ , Fe3+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

B

Boron
Cell ： PACKTEST Square Cup
Wavelength ： 490 nm

Color development： Light yellow → Yellow
Method： Azomethine H
Range： 0.50 ー 6.00 mg/L（ppm）
Reagent： WAK-B Tube
Reaction time ： 40 minutes after drawing sample into the tube.

Procedure
1. Press【B】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Shake the tube in Step 5 about 10 times. (If large orange lumps
are left in the tube, further shake the tube.)（Fig.4）

6WDUW
Fig.3

Fig.4

7. Gently return the solution in the tube to the Cell, set it again in thecell box.（Fig.5）
8. After 40 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

Caution
1. In this method, the concentration of ionized borate (borax) is measured and it is converted into a boron concentration value.
It is impossible to measure the concentration of fluoroborate (BF4－ ).
2. The optimum pH during color development is 6. If the pH of the sample is not within the range from 5 to 9, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
					 15℃・・・×0.95
25℃・・・×1.25

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing it withthe official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement. (However,ordinary seawater contains boron at 4 to 5 mg/L.)－ 19 －≤ 1000mg/ L ,:	As（Ⅲ）, Cl − , F − , I － , K+ , Na+ , NH 4+ , NO2 － , NO3 － ,PO 43 － , Phenol≤ 500mg/L,: Mg2+ , Mn2+
≤ 250mg/L,: Ni2+ , SO 42 － , Zn2+
≤ 100mg/L,: Ba2+ , Ca2+
≤ 50mg/L,: Al3+ , Cr 3+ , Anionic Surfactant≤ 25mg/L,: Cu2+
≤ 10mg/L,: CN － , Cr（Ⅵ）, Residual Chlorine≤ 5mg/L,: Fe2+ , Sn2+
< 1mg/L,: Ag+ , Fe3+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cd

Cadmium

Cell ： PACKTEST Square Cup
Color development： Yellow → Orange → Pink
Wavelength ： 554 nm
Method： Separation with a syringe filter / 5-Br-PAPS
Range： 0.003 ー 0.035 mg/L(ppm)
Reagent： SPK-Cd K-1（Liquid）, K-2（Liquid）, K-3（Liquid）, K-4（Liquid）, Tube
Reaction time ： 1 minute after drawing sample into the tube.
Preparation Procedure： Read the instruction for the reagent (SPK-Cd).
0.5

Procedure.3

1. Press【Cd】.

6\ULQJH&RQQHFW HDFKSDUWVrUPO\WR
SUHYHQW IURPOHDNLQJ

2. Press【OK】to switch to the photometry window.

7UHDWHG)LOWHU.

3. Perform procedures of "Washing" in the instruction supplied with thereagent (SPK-Cd).

=HUR

1R]]OH

Fig.1

Fig.2

4. Press the treated filter onto the syringe firmly, and connect thenozzle.（Fig.1）

Fig.3
1.0
mL

1.0
mL

5. Fill the Cell with K-4 Reagent for 1.5 mL (up to line).（Fig.2）

0.5
.3

0.5

6. Put the Cell in the cell box and press【Zero】.（Fig.3）

.3

'UDZ
,Q

8. Press out the solution completely to the Cell.（Fig.5）

1.0mL

7. Draw the solution slowly from the Cell into the syringe, as much as
possible. Press out the solution slowly to the Cell.
		 Repeat this step 4 times.（Fig.4）

6PDOODPRXQW
RI VROXWLRQZLOOUHPDLQ

3UHVV2XW

 WLPHV

Fig.4

0.5
.3

9. Suck the whole amount of the solution in the Cell into the tube andpress【Start】at the same time. （Fig.6）
10.Shake the tube in Step 9 about 30 times to dissolve the reagent in
the tube completely. （Fig.7）
11.Return the solution back into the Cell slowly, and set the Cell into thecell box. （Fig.8）

3UHVV2XW&RPSOHWHO\,I VROXWLRQ UHPDLQVLQVLGH WKH QR]]OHUHPRYHWKHV\ULQJHGUDZ LQ VRPH DLU
FRQQHFW EDFN DQG
SUHVVRXWWKHDLU
溶出液

Fig.5

6WDUW
Fig.6

12.After 1 minute has elapsed, the concentration will be automaticallydisplayed.
Dissolve the reagent
completely

Fig.7

Fig.8

Caution
1. Refer to the instruction for the reagent (SPK-Cd).
2. Perform measurement with the solution temperature set to 15 to 30℃ .

Influence of coexisting substance
Refer to the instruction for the reagent (SPK-Cd).

－ 20 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cl-500

Chloride（High Range）
Cell ： PACKTEST Square Cup
Wavelength ： 615 nm

Color development： Transparent → White Turbidity
Method： Dilution and Turbidimetry with Silver Nitrate
Range： 20 ー 500 mg/L(ppm)
Reagent： DPR-Cl R-1（Dropper）, R-2（Dropper）
Reaction time ： 3 minutes after R-2 reagent is added.

Procedure
1. Press【Cl-500】.

0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0

mL

0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0

mL

0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0

3. Suck about 0.2mL of air into the supplied syringe, suck the sample insuccession, and adjust the liquid level to the scale mark of 0.12mL.
（Fig.1）mL

2. Press【OK】to switch to the photometry window.

P/
V\ULQJH$LU$GMXVWHG
WRP/-XVWP/
LQFOXGLQJWKHWLS

6DPSOH

6DPSOH

Fig.1

Fig.2

4. Pour the sample in the syringe into the Cell.（Fig.2）
5. Add pure water up to line of the Cell by using supplied pipette.（Fig.3）
6. Put the Cell in the cell box and press【Zero】.（Fig.4）
3XUH
ZDWHU

7. Add one droplet of the R-1 reagent, attach the cap, and shake the
Cell 2 to 3 times.（Fig.5）=HUR

8. Add one droplet of the R-2 reagent, immediately attach the cap, andshake the Cell 2 to 3 times, and press【Start】.（Fig.6）

Fig.3

Fig.4

9. Remove the cap of the Cell, set the Cell in the cell box again.（Fig.7）
10. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

5

5

6WDUW
Fig.5

Fig.6

Fig.7

Caution
1. Refer to "Cl Chloride".
2. In Step 5 of "Procedure", pure water is required.（Do not use tap water.）
3. Use of a measuring pipette or the like instead of the supplied syringe enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing it withthe official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

－ 21 －≤ 10000mg/L,:	Al3+ , B（Ⅲ）, Ca2+ , Co2+ , Cr 3+ , Cu2+ , F － , Fe3+ , K+ ,Mg2+ , Mn2+ , Na+ , NH 4+ , Ni2+ , NO2 － , NO3 － , SO 42 － ,Zn2+ , Phenol≤ 5000mg/L,: Silica≤ 2000mg/L,: PO 43 － , Anionic Surfactant≤ 100mg/L,: Fe2+
≤ 50mg/L,: Residual Chlorine≤ 10mg/L,: Ba2+ , Cr（Ⅵ）, I － , Mo（Ⅵ）< 1mg/L,: CN －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cl

Chloride
Cell ： PACKTEST Square Cup
Wavelength ： 615 nm

Color development： Transparent → White Turbidity
Method： Turbidimetry with Silver Nitrate
Range： 2.0 – 50.0 mg/L(ppm)
Reagent： DPR-Cl R-1（Dropper）, R-2（Dropper）
Reaction time ： 3 minutes after R-2 reagent is added.

Procedure
1. Press【Cl】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add one droplet of the R-1 reagent, attach the cap, and shake the
Cell 2 to 3 times.（Fig.3）
6. Add one droplet of the R-2 reagent, immediately attach the cap, andshake the Cell 2 to 3 times, and press【Start】.（Fig.4）

5

5

6WDUW
Fig.3

Fig.4

7. Remove the cap of the Cell, set the Cell in the cell box again.（Fig.5）
8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

Caution
1. In this method, the concentration of chloride ions (Cl － ) in the sample is measured. If Br － and I － coexist, a positive measurement
error could occur.
2. The chloride to be measured is not such chlorine for disinfection as contained in tap water or the like. To measure theconcentration of chlorine for disinfection, refer to "ClO-DPD Residual Chlorine (Free)".
3. The optimum pH during color development is 9 or less. To an alkaline sample, add dilute sulfuric acid or the like so as to adjust the
pH of the sample to 9 or less. (Do not use hydrochloric acid.)
4. Perform measurement with the sample temperature set to 20 to 25℃ .
		 If the sample temperature is not within the range, multiplying the measurement value by either of the following coefficients canimplement correction.
					 15℃・・・×1.3
30℃・・・×0.84
5. Depending on the operation method, the results vary. In Step 6 of "Procedure", shake the Cell as soon as possible after R-2
reagent is added.
6. To set the Cell in the box, remove the cap. Wipe off water droplets before setting the Cell in the cell box.
7. As turbid substances attach to the Cell after measurement, thoroughly clean the Cell.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , Co2+ , Cr 3+ , Cu2+ , F － , Fe3+ , K+ ,Mg2+ , Mn2+ , Na+ , NH 4+ , Ni2+ , NO2 － , NO3 － , SO 42 － ,Zn2+ , Phenol≤ 500mg/L,: Silica≤ 200mg/ L ,:	PO 43 － , Anionic Surfactant≤ 10mg/L,: Fe2+
≤ 5mg/L,: Residual Chlorine≤ 1mg/L,: Ba2+ , Cr（Ⅵ）, I － , Mo（Ⅵ）< 1mg/L,: CN －It is possible to measure seawater, but as it has a high concentrationof chloride ions, dilution is necessary. (Approximately 1000 times
in the case of artificial seawater)－ 22 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

ClO-C

Residual Chlorine (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 470 nm, 600 nm

Color development： None → Yellow → Orange → Red brown
Method： Potassium Iodide
Range： 2 ー 500 mg/L(ppm)
Reagent： WAK-ClO（C） Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【ClO-C】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 1 minute has elapsed, the concentration will be automaticallydisplayed.

Caution
1. In this method, the concentration of the total residual chlorine (free residual chlorine + combined residual chlorine) is
measured.
2. This residual chlorine is chlorine for disinfection. To measure the concentration of chloride ions (Cl － ) such as common salt,
refer to "Cl Chloride".
3. The optimum pH during color development is 4. If the pH of the sample is not within the range from 3 to 9, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Reductive substances such as Fe2+ and NO2 － consume residual
chlorine.
NO 2 － may serve as an oxidizer and may cause a positive
measurement error.
Oxidizing substances such as hydrogen peroxide cause apositive measurement error.

－ 23 －≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , Cl − , F − , K+ , Mg2+ , Mn2+ , Mo（Ⅵ）,
Na+ , NH 4+ , Ni2+ , NO3 − , PO 43 − , SO 42 − , Zn2+
≤ 200mg/L,: Ba2+
≤ 50mg/L,: Cr（Ⅵ）, Anionic Surfactant, Phenol≤ 20mg/L,: Cr 3+≤ 5mg/L,: Fe3+
≤ 2mg/L,: Cu2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

ClO-DPD

Residual Chlorine (Free)
Cell ： PACKTEST Square Cup
Wavelength ： 552 nm, 532 nm, 670 nm

Color development： None → Pink
Method： N,N-diethyl-p -phenylenediamine sulfate
Range： 0.05 ー 3.00 mg/L(ppm)
Reagent： WAK-ClO・DP Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【ClO-DPD】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, immediately returnthe solution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 1 minute has elapsed, the concentration will be automaticallydisplayed.

Caution
1. In this method, the concentration of free residual chlorine in the sample is measured.
		 To measure the concentration of the total residual chlorine (= free residual chlorine + combined residual chlorine), refer to"T-ClO Total Residual Chlorine".
2. This residual chlorine is chlorine for disinfection. To measure the concentration of chloride ions (Cl － ) such as common salt,
refer to "Cl Chloride".
3. The optimum pH during color development is 7. If the pH of the sample is not within the range from 5 to 9, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .
5. When the concentration of residual chlorine is 500 mg/L or higher, the measurement value will be low. If high concentration
is anticipated, dilute in advance and then perform measurement.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Reductive substances such as CN － , Fe2+ and NO2 － consume
residual chlorine.
Oxidizing substances cause a positive measurement error.
As NH 4 + reacts with free residual chlorine to turn into
combined residual chlorine, the amount of free residual
chlorine reduces.
(The total residual chlorine remains the same.)
If I － coexists, combined residual chlorine will also be measured.

－ 24 －≤ 1000mg/ L ,:	Ca2+ , Cl − , F − , K+ , Mo（Ⅵ）, Na+ , Ni2+ , PO43 − , SO42 − , Zn2+
≤ 500mg/L,:	Al3+ , B（Ⅲ）, Cr 3+ , Mg2+
≤ 250mg/L,: Mn2+
≤ 100mg/L,:	NO3 − , Phenol≤ 25mg/L,: Co2+
≤ 10mg/L,: Fe3+
≤ 5mg/L,: Ba2+
≤ 1mg/L,: Cu2+
< 1mg/L,: Ag+ , Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

T-ClO

Total Residual Chlorine
Cell ： PACKTEST Square Cup
Wavelength ： 552 nm, 532 nm, 670 nm

Color development： None → Pink
Method： N,N-diethyl-p -phenylenediamine sulfate
Range： 0.05 ー 3.00 mg/L(ppm)
Reagent： WAK-T・ClO Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【T-ClO】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, immediately returnthe solution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

Caution
1. In this method, the concentration of total residual chlorine in the sample is measured.
		 To measure the concentration of free residual chlorine (= total residual chlorine - combined residual chlorine), refer to "ClO-DPD
Residual Chlorine (Free)".
2. Total residual chlorine is chlorine for disinfection. To measure the concentration of chloride ions (Cl － ) such as common salt, refer
to "Cl Chloride".
3. The optimum pH during color development is 7. If the pH of the sample is not within the range from 5 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .
5. When the concentration of residual chlorine is 500 mg/L or higher, the measurement value will be low. If high concentration is
anticipated, dilute in advance and then perform measurement.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Reductive substances such as CN － , Fe2+ and NO2 － consume
residual chlorine.
Oxidizing substances cause a positive measurement error.

－ 25 －≤ 1000mg/ L ,:	Ca2+, Cl − , F − , I − , K+, Mo（Ⅵ）, Na+, Ni2+, PO43 − , SO42 − , Zn2+
≤ 500mg/L,:	Al3+ , B（Ⅲ）, Cr 3+ , Mg2+
≤ 250mg/L,:	Mn2+≤ 100mg/L,:	NO3 − , Phenol≤ 25mg/L,:	Co2+≤ 10mg/L,: Fe3+
≤ 5mg/L,: Ba2+
≤ 1mg/L,: Cu2+
< 1mg/L,: Ag+ , Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

ClO 2Chlorine Dioxide
Cell ： PACKTEST Square Cup
Wavelength ： 552 nm, 532 nm, 670 nm

Color development： None → Pink
Method： N,N-diethyl-p -phenylenediamine sulfate with Glycine
Range： 0.20 ー 6.00 mg/L(ppm)
Reagent： WAK-ClO2 K-1（Dropper）, Tube
Reaction time ： 30 seconds after drawing sample into the tube.

Procedure
1. Press【ClO 2 】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add two droplets of K-1 reagent, attach the cap, and shake the Cell 2
to 3 times.（Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.4）

.

6WDUW
Fig.3

Fig.4

7. Lightly shake the tube in Step 6 from 5 to 6 times, immediately returnthe solution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.5）
8. After 30 seconds have elapsed, the concentration will be automaticallydisplayed.
Fig.5

Caution
1. Strictly observe the reaction time. After the reaction time has elapsed, the color development will be intensified. In particular, inthe case where residual chlorine, chlorite ions, chlorate ions, etc. are considered to coexist, be sure to strictly observe the reactiontime.
2. The optimum pH during color development is 7. If the pH of the sample is not within the range from 5 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .
4. When the concentration of chlorine dioxide is 200 mg/L or higher, the measurement value will be low. If high concentration is
anticipated, dilute in advance and then perform measurement.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Reductive substances such as CN － , Fe2+, and NO2 － consume
chlorine dioxide. Oxidizing substances cause a positive
measurement error.
If I － coexists, residual chlorine will also be measured.

－ 26 －≤ 1000mg/ L ,:	B（Ⅲ）, Ca 2+ , Cl − , Cr 3+ , F − , I － , K+ , Mg 2+ , Mn 2+ ,Na+ , Ni2+ , NO3 − , PO 43 − , SO 42 − , Zn2+
≤ 500mg/L,:	Al3+ , Chlorite ion , Chlorate ion≤ 250mg/L,: NH 4+≤ 50mg/L,: Mo（Ⅵ）≤ 25mg/L,: Co2+
≤ 10mg/L,: Fe3+ , Phenol≤ 5mg/L,: Ba2+
≤ 1mg/L,: Cu2+ , Residual Chlorine< 1mg/L,: Ag+ , Cr（Ⅵ）, Fe2+ , NO2 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NaClO 2

Sodium Chlorite
Cell ： PACKTEST Square Cup
Wavelength ： 470 nm, 600 nm

Color development： None → Yellow → Orange → Red brown
Method： Potassium Iodide
Range： 2 ー 500 mg/L(ppm)
Reagent： WAK-NaClO2 K-1（Dropper）, Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【NaClO2】
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

5. Add four droplets of K-1 reagent.（Fig.3）

Fig.2'RQRWVKDNH

6. Immediately suck the whole amount of the sample in the Cell into thetube and press【Start】at the same time.（Fig.4）
7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）

.

6WDUW
Fig.3

Fig.4

8. After 1 minute has elapsed, the concentration will be automaticallydisplayed.
Fig.5

Caution
1. In this method, the concentration of residual chlorine and chlorine dioxide is also measured.
2. As chlorine gas may be generated during measurement, be sure to perform measurement while ventilating the air.
3. The optimum pH during color development is 1. Neutralize a sample of pH 10 or greater with dilute sulfuric acid.
4. Perform measurement with the sample temperature set to 15 to 30℃ .
5. After the K-1 reagent is added in Step 5 of "Procedure", do not shake the Cell, but immediately suck the sample into the tube.
		 If the Cell is shaken or it takes time before the sample is sucked, the result may become low.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater and tap water do not affect the measurement.
The residual chlorine and chlorine dioxide also make color
development and cause possitive error. Oxidizing substancessuch as hydrogen peroxide cause a positive measurement error.
Reductive substances such as Fe2+ and NO2 － consume sodium
chlorite. NO 2 － may serve as an oxidizer and may cause apositive measurement error.
When the sample contains starch, the color may develop tobrown to black, disabling measurement.

－ 27 －≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , F − , I − , K+ , Mg2+ , Mn2+ , Mo（Ⅵ）,
Na+ , NH4+ , Ni2+ , NO3 − , PO43 − , SO42 − , Zn2+ , Amino acid ,Glucose , Phenol , Silica , Sodium Chlorate≤ 100mg/L,:	Anionic Surfactant≤ 20mg/L,: Albumin≤ 10mg/L,: Ba2+ , Starch≤ 5mg/L,: Cu2+ , Fe3+
≤ 1mg/L,: Fe2+ , Residual Chlorine< 1mg/L,: Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NaClO 2-D

Sodium Chlorite (Low Range)

Cell ： PACKTEST Square Cup
Color development： None → Pink
Wavelength ： 552 nm, 532 nm, 670 nm
Method： Potassium iodide and DPD method
Range： 0.10 ー 2.00 mg/L (ppm)
Reagent： WAK-NaClO2（D）, K-1（Small Pack）, K-2（Dropper）, K-3（Dropper）, Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【NaClO2-D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add the K-1 reagent. （Fig.3）
6. Add one droplet of K-2 reagent, attach the cap, and shake the Cell 10times. （Fig.4）

.

K-2

Fig.3

7. Add two droplets of K-3 reagent. （Fig.5）
8. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.6）
9. Lightly shake the tube in Step 8 from 5 to 6 times, immediately returnthe solution in the tube to the Cell in a gentle manner, set it again in
the cell box. （Fig.7）

Fig.4'RQRWVKDNH

.

6WDUW
Fig.5

Fig.6

10. After 1 minute has elapsed, the concentration will be automaticallydisplayed.

Fig.7

When separately measuring the concentration of sodium chlorite and residual chlorine（0.5 mg/L or less）① Press【NaClO2-D】.
② Press【OK】to switch to the photometry window.
③ Fill the Cell with the sample for 1.5 mL (up to line). （Fig.8）④ Suck the whole amount of the sample in the Cell into the tube of
PACKTEST Total Residual Chlorine (WAK-T･ClO) and then lightly
shake the tube 5 to 6 times.
		 Develop the color of the total residual chlorine. (At this point, thecolor of the sodium chlorite will not be developed.)（Fig.9）

Fig.8

Fig.9

⑤ Return the solution in the tube to the Cell, put the Cell in the cell box,
and press【Zero】.（Fig.10）=HUR⑥ Take the Cell out of the cell box, empty it, and clean it with purewater or the sample.（Fig.11）

Fig.10

Fig.11⑦ Fill the Cell in ⑥ with the sample for 1.5 mL (up to line). （Fig.12）⑧ From this step onwards, perform measurement by following in Step 5of "Procedure".

Fig.12－ 28 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Caution
1. In this method, the concentration of residual chlorine (hypochlorous acid, etc.) and chlorine dioxide is also measured.
		 To separately measure the concentration of sodium chlorite and residual chlorine, refer to "When separately measuring theconcentration of sodium chlorite and residual chlorine (0.5 mg/L or less)." In this case, separately prepare the PACKTEST Total
Residual Chlorine (WAK-T･ClO). However, this cannot be applied in the case where residual chlorine of 0.5 mg/L or more coexists.
2. As chlorine gas may be generated during measurement, be sure to perform measurement while ventilating the air.
3. The pH as of after adding K-2 reagent is approx. 1. The pH as of during color development is approx. 5. If the pH of the sample
is not within the range from 2 to 9, perform measurement after neutralizing the sample with dilute sulfuric acid or dilute sodiumhydroxide solution, etc. However, extract by 9 mmol/L sodium carbonate solution can be measured without pH adjustment.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
The color is developed by the residual chlorine contained in the
tap water. The residual chlorine and chlorine dioxide also makethe same color development.
The color is also developed by oxidizing substances such ashydrogen peroxide.
Reductive substances such as Fe2+ and NO2 － consume sodium
chlorite. NO2 － may serve as an oxidizer and may develop its
color.

－ 29 －≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , CI − , F − , I − , K+ , Mg2+ , Mn2+ , Mo（Ⅵ）,
Na+ , NH4+ , Ni2+ , NO3 − , PO43 − , SO42 − , Zn2+ , Albumin ,Sodium Chlorate , Citric Acid , Glycine , Glucose ,Glutamic Acid , Tartaric Acid , Silica , Starch , Phenol≤ 500mg/L,:	Co2+≤ 50mg/L,: Anionic Surfactant≤ 10mg/L,: Fe3+
≤ 2mg/L,: Cu2+ , Cationic Surfactant< 1mg/L,:	Cr（Ⅵ）, Fe2+ , NO 2 − , SO32 − , Ascorbic Acid ,Residual Chlorine , Hydrogen Peroxide , Chlorine DioxideⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

CN-2Free Cyanide
Cell ： PACKTEST Square Cup
Wavelength ： 607 nm, 535 nm, 680 nm

Color development： None →（Red）→ Blue
Method
： 4-Pyridinecarboxylic Acid
Range
： 0.01 ー 1.00 mg/L(ppm)
Reagent： WAK-CN-2 K-1（Small Pack）, Tube
Reaction time ： 10 minutes after drawing sample into the tube.

Procedure
1. Press【CN-2】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add the K-1 reagent, attach the cap, and shake the Cell 5 to 6
times. （Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.4）

.

6WDUW
Fig.3

Fig.4

Fig.5

Fig.6

7. Lightly shake the tube in Step 6 about 10 times. （Fig.5）
8. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box. （Fig.6）
9. After 10 minutes have elapsed, the concentration will be
automatically displayed.

Caution
1. In this method, the concentration of free cyanide (mainly cyanide ions (CN － ) and cyanogen chloride (CNCl)) in the sample ismeasured. To measure the concentration of total cyanide including iron cyano complex, etc. refer to "CNT-D Total Cyanide (Low
Range)" and "CNT Total Cyanide".
2. The optimum pH during color development is 6. If the pH of the sample is not within the range from 5 to 12, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. After the tube is shaken in Step 7 of "Procedure", immediately and gently return the solution in the tube to the Cell. If you shakethe tube excessively or take time before returning the solution, turbid may be generated.
4. Perform measurement with the sample temperature set to 20 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
So far, only some thiocyanogens and ethylene amines(tetraethylenepentamine and pentaethylene hexamine) areerroneously detected.
If a strong oxidizing substance such as residual chlorine or areductive substance such as sulfite exists, a negative error occurs.
It is not possible to measure seawater.
If the sample is industrial wastewater or the like that contains
interfering substance, conduct pretreatment by distillation/
ventilation method or others.

－ 30 －≤ 1000mg/ L ,:	As（Ⅲ）, B（Ⅲ）, Cl − , F − , K+ , Mg2+ , Mo（Ⅵ）, Na+ , NH 4+, NO3 − , PO 43 − , SO 42 − , EDTA , Phenol≤ 200mg/L,:	NO2 − , Zn2+
≤ 100mg/L,:	Ca2+ , Ascorbic Acid , Anionic Surfactant , Silica≤ 50mg/ L,: Al3+ , Cr 3+ , Cr（Ⅵ）≤ 20mg/L,: Cu2+
≤ 10mg/L,: Ba2+ , Fe2+ , Fe3+
≤ 5mg/L,: Residual Chlorine≤ 1mg/L,: Formaldehyde< 1mg/L,:	Co2+ , I − , Mn2+ , Ni2+ , SCN − , SO32 − , 		some kinds of ethylene amine , Cationic SurfactantⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

CN T

Total Cyanide

Color development： Yellow → Orange → Brown
Method： Distillation and Picric acidRange
： 0.1 ー 3.0 mg/L(ppm)
Reagent： LR-CNT No.46 R-1（Powder）, R-2（Pack）
Reaction time ： 0 minute
Other Items to Use ： Water Analysis Set: Total Cyanide (Model: WA-CNT or WA-CNT-2)
Preparation Procedure： Read the instruction for "Water Analysis Set: Total Cyanide".

Cell ： PACKTEST Square Cup
Wavelength ： 540 nm

Procedure
1. Press【CNT】.
2. Press【OK】to switch to the photometry window.
Pure water

3. Fill the Cell with pure water for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

5. Take out the Cell, discard the pure water, and pour 1.5 mL of thesolution that has been adjusted to 25 mL through distillation andcolor development with the Water Analysis Set: Total Cyanide to thesame Cell. （Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）

Fig.2

7KHVDPH&HOO

3XUHZDWHU

7. The concentration will be automatically displayed.
Fig.3

6ROXWLRQ

6WDUW
Fig.4

Caution
1. As the glass portion of the distiller becomes hot during distillation, be careful not to get burned.
2. If you use dilute sulfuric acid instead of R-1 reagent, be sure to put a boiling stone in the flask to avoid bumping in it.
3. Sufficiently ventilate the room during distillation.

Influence of coexisting substance
Refer to the instruction for "Water Analysis Set: Total Cyanide."－ 31 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

CN T-DTotal Cyanide (Low Range)

Cell ： PACKTEST Square Cup
Color development： None →（Red）→ Blue
Wavelength ： 638 nm, 590 nm
Method： Distillation and 4-Pyridinecarboxylic Acid - Pyrazolone
Range： 0.005 ー 0.150 mg/L(ppm)
Reagent： LR-CN-B No.14B R-1（Small Pack）, R-2（Pack）
Reaction time ： 20 minutes after R-2 reagent is added.
Other Items to Use ： Water Analysis Set: Total Cyanide (Low Range) (Model: WA-CNT(L))
Preparation Procedure： Read the instruction for pretreatment reagent (CNT-RA).

Procedure
1. Press【CNT-D】.
2. Press【OK】to switch to the photometry window.
Pure water

3. Fill the Cell with pure water for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）
		 Discard the pure water in the Cell.

Fig.1

Fig.2

Solution

5. Add the R-1 reagent to the Round Cell containing the solution as
explained in the instruction for pretreatment reagent (CNT-RA). （Fig.3）
6. Tightly attach the cap, shake the Round Cell about 10 times, andwait for 3 minutes.（Fig.4）

5

Fig.3

Fig.4

7. Add the R-2 reagent to the Round Cell, press【Start】, tightly attach
the cap, and immediately shake the Round Cell strongly more than 60
times in 30 seconds. （Fig.5）5

8. Within 20 minutes, pour the solution in the Round Cell for 1.5 mL into
the Cell that has gone through zero adjustment (up to line) and put
the Cell in the cell box. （Fig.6）

6WDUW
Fig.5

9. After 20 minutes have elapsed, the concentration will be automaticallydisplayed.

図6

CAUTION
1. Perform measurement with the solution temperature set to 15 to 30℃ .
2. As the R-2 reagent dissolves only partially, after shaking the Round Cell in Step 6.

－ 32 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

COD

COD with KMnO 4Cell ： PACKTEST Square Cup
Color development： Red purple → Green
Wavelength ： 525 nm
Method： Oxidation by potassium permanganate in alkaline
Range： 2.0 ー 10.0 mg/L(ppm)
Reagent： LR-COD-B-2 No.44 R-1（Liquid）, R-2（Liquid）, Neutralizer（Dropper）
Reaction time ： 10 minutes after R-2 reagent is added.

Procedure
1. Press【COD】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line) and fill the Round
Cell with the sample for 25 mL (up to white line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】. Discard the sample in
the Cell.（Fig.2）

Fig.1

Fig.2

5. Add 0.5 mL of R-1 reagent into the Round Cell using the supplied
syringe, tightly attach the cap, and stir the solution 5 to 6 times.（Fig.3）
6. Add 1mL of R-2 reagent into the Round Cell using the supplied pipette,and press【Start】.（Fig.4）
7. Tightly attach the cap and stir the solution 5 to 6 times.（Fig.5）

5

5

6WDUW
Fig.3

Fig.4

Fig.5

Fig.6

8. Within 10 minutes, pour 1.5 mL of the solution in the Round Cell into
the Cell that has gone through zero adjustment, and put the Cell in
the cell box. (At this point, clean the Cell with the solution containedin the Round Cell.)（Fig.6）
9. After 10 minutes have elapsed, the concentration will be automaticallydisplayed.
10. Dispose of the wastewater in the Round Cell as of after measurementby adding about 8 droplets (0.5 mL) of neutralizer to it and confirmingthat it has become approximately neutral.

CAUTION
1. The optimum pH during color development is 12 or more. To an acid sample, add dilute sodium hydroxide solution or the like so as
to adjust the pH of the sample to 6 or more.
2. Perform measurement with the sample temperature set to 15 to 25℃ .
3. Before filling the Cell with the solution in the measurement procedure Steps 8, rinse the Cup with the solution 2 to 3 times.
4. It is not possible to measure seawater.

Positioning of this measurement method with respect to JIS method
In Japan, the potassium permanganate method (CODMn) at 100℃ for 30 minutes according to JIS K 0102 17 is commonly used for
management of industrial wastewater, but the measurement method we offer applies the alkali method (CODOH) according to JIS K
0102 19 and allows simple measurement in a short period of time.
In the JIS alkali method, the amount of potassium permanganate that has been consumed in a boiled water bath for 20 minutes isobtained through titration. On the other hand, the measurement method we offer obtains the amount of potassium permanganatethat has been consumed at a room temperature for 10 minutes by converting the decrease of absorbance into a COD value.
Verification is conducted by using glucose reference solution, but the degree of oxidation of oxidized substance in the sample bypotassium permanganate differs depending on the type and the amount of the substance.
In addition, as the reaction conditions and measurement conditions differ even between the alkali method and the acid method, thevalues obtained by this measurement method are only approximate values and therefore due attention needs to be paid when thismethod is used for measurement of industrial wastewater.
As the value obtained by this method and that obtained by JIS method may not coincide with each other, use this method after
obtaining the relationship between those methods.

－ 33 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

ColorCell ： PACKTEST Square Cup
Wavelength ： 460 nm

Measurement of degree of yellow color of sample
Calibration： Pt-Co color standard solution
Range： 50 to 1000 degrees
Reaction time ： 0 minute

Procedure
1. Press【Color】.
2. Press【OK】to switch to the photometry window.
Pure water

3. Fill the Cell with pure water for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Take out the Cell, discard the pure water, fill the same Cell with 1.5 mLof sample. （Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）
7KHVDPH&HOO
3XUHZDWHU

7. The result will be automatically displayed.

Fig.3

6WDUW
Fig.4

CAUTION
1. This method is applied only to substances in pale yellow to brownish yellow colors existing in a dissolved state in water or in a
colloidal state, and other color and turbid interfere the measurement.

－ 34 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cr 6+

Chromium (Hexavalent)
Cell ： PACKTEST Square Cup
Wavelength ： 542 nm, 580 nm, 670 nm

Color development： None → Light red → Red → Red purple
Method： Diphenylcarbazide
Range： 0.05 ー 1.50 mg/L(ppm)
Reagent： WAK-Cr6+ Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【Cr6＋】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of hexavalent chromium (Cr6+) in the sample is measured.
		 To measure the concentration of the total chromium including trivalent chromium (Cr3+), refer to "CrT Total Chromium".
		 If a reductive substance coexists in the sample, hexavalent chromium is reduced to trivalent chromium. In this case, performmeasurement of the total chromium.
2. The optimum pH during color development is 2 or less. Turn a sample of pH9 or greater to neutral or lower with dilute sulfuric acidor the like. In particular, if the sample is wastewater from fresh concrete plant or the like whose pH is high, pay special attention.
3. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
If a reductive substance coexists in the sample, hexavalentchromium is reduced to trivalent chromium. In this case, performmeasurement of the total chromium.

－ 35 －≤ 1000mg/ L ,:	Ba2+ , Ca2+ , Cl − , CN − , Co2+ , I − , K+ , Mg2+ , Mn2+ , Na+ ,NH4+ , Ni2+ , NO3 − , PO43 − , SO42 − , Zn2+ , Phenol≤ 500mg/L,:	Al3+ , F −≤ 250mg/L,: B（Ⅲ）≤ 25mg/L,: NO2 −≤ 10mg/L,: Ag+≤ 5mg/L,: Cu2+ , Mo（Ⅵ）≤ 2mg/L,: Fe3+
≤ 1mg/L,: Residual Chlorine< 1mg/L,: V（Ⅴ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cr 6+-D

Chromium (Hexavalent) (Low Range)

Color development： None → Light red → Red purple
Method： Diphenylcarbazide Absorptiometry Coupled with Collecting on Membrane Filter
Range： 0.003 ー 0 .100 mg/L(ppm)
Reagent： DPRーCr6+D R-1（Pack）, R-2（Liquid）, R-3（Liquid）
Reaction time ： 0 minutePreparation Procedure： Read the instruction for the reagent (DPR-Cr6+D).

Cell ： PACKTEST Square Cup
Wavelength ： 542 nm, 580 nm, 670 nm

Procedure
1. Press【Cr6＋ -D】.
2. Press【OK】to switch to the photometry window.
3. Perform procedures ① - ⑥ of the usage for the reagent (DPR-Cr6+D).

Pure water

4. Fill the Cell with pure water (or tap water) so that the water levelexceeds the mark line (1.5 mL).（Fig.1）

Fig.1

=HUR

3XUHZDWHU

Fig.2

Fig.3

5. Put the Cell in the cell box and press【Zero】.（Fig.2）

3OXQJHU
2.5
mL

0.5

2.5
mL

2
2.5

1.5
1
0.5

mL

2
1.5
1

-XVWP/
LQFOXGLQJWKHWLS)LOWHU

0.5

9. Attach the cap of the Cell, shake the Cell 5 to 6 times while strongly
holding it, and then remove the cap.(If the cap is kept attached, thesolution leaks out.)（Fig.6）

0.5

8. Attach the filter to the 2.5 mL syringe, and extrude the R-3 reagent
one drop by one drop to collect the whole amount in the Cell. When you
have extruded almost half the R-3 reagent from the syringe, pull backthe pushing rod to the uppermost portion (scale mark of 2.5 mL). Then,slowly extrude the remaining R-3 reagent one drop by one drop. The
collection completes when the pushing rod reaches the lowermost partand the remaining R-3 reagent has been completely extruded.（Fig.5）

1.5

7. Suck about 0.5 mL of air into the 2.5 mL syringe, suck the R-3
reagent in succession, and adjust the liquid level to the scale mark of
1.9 mL.（Fig.4）

P/
V\ULQJH

2

P/
V\ULQJH$GMXVWHG
WRP/

1

6. Take out the Cell and completely discard the water in the Cell. If water
droplets exist on the inner wall, remove them by absorbing them withtissue paper or the like.（Fig.3）

$LU
5

Fig.4

7KHVDPH&HOO

Fig.5

5-6

6WDUW
Fig.6

Fig.7

10. Set the Cell in the cell box again and press【Start】.（Fig.7）
11. The concentration will be automatically displayed.

CAUTION
1. In this method, the concentration of hexavalent chromium is obtained from the absorbance of the solution that has been obtainedby using the reagent (DPR-Cr6+D). For notes on the operation, refer to the instruction for the reagent (DPR-Cr6+D).
2. The pH of the sample as of after adding R-1 reagent is 1, that of the sample as of after adding R-2 reagent is 2, and that of thesolution is 3 to 4. If the pH of the sample is not within the range from 2 to 9, neutralize the sample with dilute sulfuric acid or
dilute sodium hydroxide solution, etc.
3. As the sample as of after adding reagent is strongly acidic, wear PPE during measurement and gently proceed with the operation
procedures. Note that if the connection of the screw part between each syringe and filter is loose, the solution could leak out.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
Refer to the instruction for the reagent (DPR-Cr6+D).

－ 36 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cr T

Total Chromium

Cell ： PACKTEST Square Cup
Color development： None → Light red → Red → Red purple
Wavelength ： 542 nm, 580 nm, 670 nm
Method： Oxidation and Diphenylcarbazide
Range： 0.05 ー 1.50 mg/L(ppm)
Reagent： Pretreatment reagent（Cr-RA） R-1（Dropper）, R-2（Dropper）, R-3（Dropper）, WAK-Cr6+ Tube
Other Items to Use ： Beaker, Heater
Reaction time ： 2 minutes after drawing sample into the tube.

Pretreatment method
Conduct pretreatment by following the procedures below.

R-2

1. Fill the beaker with 15 mL of sample, and add 5 droplets of the R-1
reagent. （Fig.1）
2. While heating the sample up to a moderately boiling state, add theR-2 reagent until pale reddish violet color remains even when thesample is stirred. （Fig.2）
3. Stop heating the sample, and add one droplet of the R-3 reagent todecolor the pale reddish violet color. If the pale reddish violet color
remains, further add one droplet of R-3 reagent. （Fig.3）
		 If the amount of the sample has decreased, add pure water up to 15 mL.
4. Cool down the sample in the beaker up to 15 to 30℃ , fill the Cell with
1.5 mL of the sample (up to line). （Fig.4）

R-1

Fig.1

Fig.2

R-3

Fig.3

Fig.4

5. Measure it by following the "Procedure"

Procedure
1. Press【CrT】.
2. Press【OK】to switch to the photometry window.
3. Put the Cell containing the sample that has gone throughpretreatment in the cell box, and press【Zero】.（Fig.5）

=HUR
Fig.5

6WDUW
Fig.6

4. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.6）
5. Lightly shake the tube in Step 4 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.7）
6. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.7

CAUTION
In this method, the concentration of the total chromium including trivalent chromium (Cr 3+) and hexavalent chromium (Cr6+) ismeasured.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution.
If the influence of other substance is considered, check the measurement value by comparing it with the official method or bystandard addition method.
For details, refer to "Cr6+ Chromium (Hexavalent)".（However, it is not possible to measure seawater.）－ 37 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cu

CopperCell ： PACKTEST Square Cup
Wavelength ： 482 nm, 520 nm

Color development： None → Light orange → Orange
Method： Bathocuproine
Range： 0.10 ー 5.00 mg/L(ppm)
Reagent： WAK-Cu Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【Cu】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 1 minute has elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized univalent and divalent copper (Cu+, Cu2+) in the sample is measured.
		 If a concentration measurement value including suspension, precipitate and complex is required, dissolve the target substance inadvance and then perform measurement.
2. The optimum pH during color development is 6. If the pH of the sample is not within the range from 2 to 10, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Oxidizing substance may affect the measurement.

－ 38 －≤ 1000mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Mo（Ⅵ）, Na+ ,NH 4+ , Ni2+ , NO2 − , NO3 − , PO 43 − , SO32 − , SO 42 − ,Residual Chlorine , Phenol≤ 250mg/L,: Co2+ , Mn2+
≤ 100mg/L,: Ba2+
≤ 50mg/L,: Zn2+ , Anionic Surfactant≤ 20mg/L,: Cr 3+ , Cr（Ⅵ）≤ 10mg/L,: Ag+≤ 5mg/L,: Fe2+ , Fe3+
≤ 2mg/L,: Al3+
≤ 1mg/L,: CN −Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Cu-M-2

Copper（DDTC）
Cell ： PACKTEST Square Cup
Wavelength ： 451 nm, 500 nm, 550 nm

Color development： None → Yellow Brown
Method： DDTCRange： 0.5 ー 10.0 mg/L(ppm)
Reagent： WAK-CuM-2 Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【Cu-M-2】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Lightly shake the tube in Step 5 about 10 times. （Fig.4）

6WDUW

7. Gently return the solution in the tube to the Cell, set it again in thecell box. （Fig.5）

Fig.3

Fig.4

8. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

CAUTION
1. In this method, the concentration of ionized copper (Cu2+) in the sample is measured.
2. The optimum pH during color development is 10. If the pH of the sample is not within the range from 3 to 10, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Less than 20%(w/w) of ethanol does not affect the measurement.

－ 39 －≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ba2+ , Br − , Ca2+ , Cl − , F − , I − , K+ , Mg2+ ,Mn2+ , Mo（Ⅵ）, Na+ , NH4+ , NO2 − , NO3 − , PO43 − , SO42 − ,Anionic Surfactant, Non-ionic Surfactant, Glucose,
Silica, Phenol≤ 50mg/L,: Cd2+ , Cr（Ⅵ）, Cationic Surfactant, Residual Chlorine≤ 25mg/L,: Pb2+ , Zn2+
≤ 10mg/L,: Cr 3+ , Fe3+
≤ 5mg/L,: Fe2+ , Ni2+
≤ 1mg/L,: Co2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

DET

Anionic Surfactants
Cell ： PACKTEST Square Cup
Wavelength ： 620 nm

Color development： None → Light blue → Blue
Method： Methylene Blue - Anion surfactant Complex
Range： 0.05 ー 1.20 mg/L(ppm)
Reagent： WA-DET R-1（Dropper）, R-2（Liquid）
Reaction time ： 0 minutePreparation Procedure： Read the instruction for the reagent (WA-DET).

Pretreatment method
1. By following the procedure Step ④ in the usage supplied with the reagent(WA-DET), add 1.5 mL of the R-2 reagent using the pipette. (Theaddition amount of the R-2 reagent differs from that in the case ofvisual colorimetry.)（Fig.1）← 1.5mL

R-2

2. Fit the cap to the tube and intensely shake it so that the R-2 reagent
spreads across the whole wall surface of the tube, and use the liquid
as the solution.（Fig.2）

Fig.2

Fig.1

Procedure
1. Press【DET】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with pure water (or tap water) for 1.5 mL (up to line).（Fig.3）

Pure water

=HUR
Fig.3

Fig.4

4. Put the Cell in the cell box and press【Zero】.（Fig.4）
5. Take out the Cell, discard the pure water, and pour the whole amountof the prepared solution to the same Cell.（Fig.5）
6. Set the Cell in the cell box again and press【Start】.（Fig.6）
7. The concentration will be automatically displayed.

6WDUW
Fig.5

Fig.6

CAUTION
1. Even existence of a small amount of cationic surfactants agent, nonionic surfactants and oil can cause a negative measurement
error. If these substances are considered to coexist at high concentration, dilute the sample by 10 times in advance and thenperform measurement, and multiply the obtained value by 10.
		 *Dilution method: Fill the tube with 2 mL of the sample, and add pure water (or tap water) up to the mark line on the tube.
		 *If river water containing a non-ionic surfactants at a concentration of 1 mg/L or higher as of before adding reagent is intensely
shaken, tiny bubbles will be formed on the water surface.
2. In river water, whose concentration of DET is 0.5 mg/L or higher, coexistent substances such as non-ionic surfactants are highly
likely to exist, and the actual concentration of anionic surfactants may be much higher than the result.
3. The optimum pH during color development is 7. If the pH of the sample is not within the range from 3 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. "Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
					 10℃・・・×0.75
15℃・・・×0.85					 25℃・・・×1.25
30℃・・・×1.85
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
≤ 100mg/L,:	Ca2+ , Cl − , Cu2+ , F − , K+ , Mg2+ , Na+ , NH 4+ , NO2 − ,NO3 − , PO 43 − , SO 42 − , Residual Chlorine≤ 10mg/L,: Fe2+ , Fe3+

It is not possible to measure seawater.

－ 40 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

F

Fluoride (Free)
Cell ： PACKTEST Square Cup
Color development： Red → Purple
Wavelength ： 616 nm, 521 nm
Method： Lanthanum-Alizarin Complexon
Range： 0.40 ー 1.50 mg/L(ppm)
Reagent： WAK-F Tube
Reaction time ： 10 minutes after drawing sample into the tube.
						 （Reaction time has been reduced from calibration curve data Ver. 2.02.0.）

Procedure
1. Press【F】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Shake the tube in Step 5 about 10 times.（Fig.4）

6WDUW

7. Gently return the solution in the tube to the Cell, set it again in thecell box.

Fig.3

Fig.4

8.After 10 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

CAUTION
1. In this method, a sample containing small amount of coexistent substance such as distillation-separated extract and natural wateris handled and the concentration of ionized fluorine (F ー ) is measured.
		 It is impossible to measure the concentration of fluoroborate (BF4 ー ). Implement the distillation procedures in the pretreatment
before total fluorine measurement according to JIS K 0102 34.1.
2. The optimum pH during color development is 5. If the pH of the sample is not within the range from 3 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .
4. When the concentration of fluorine ions is 100 mg/L or higher, the measurement value will be low. If high concentration is
anticipated, dilute in advance and then perform measurement.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
It is not possible to measure seawater.
Fluorine combines with metallic element such as aluminum andiron to form a fluorocomplex, and combines with alkaline earth
metal such as calcium to exist in the form of suspended solidor precipitate of fluoride, which may not be measured by thismethod.

Except for Heavy metal ions:≤ 100mg/L,:	B（Ⅲ）, Cl − , I − , K+ , Mg2+ , Na+ , NH 4+ , NO2 − , NO3 − ,PO 43 − , SO 42 − ,Anionic Surfactant , Phenol≤ 50mg/L,: Residual Chlorine≤ 10mg/L,: Ca2+
Heavy metal ions:－ 41 －≤ 10mg/L,: Ba2+ , CN − , Cr 3+ , Cr（Ⅵ）, Mn2+
≤ 1mg/L,: Fe2+ , Fe3+ , Mo（Ⅵ）< 1mg/L,: Al3+ , Co2+ , Cu2+ , Ni2+ , Zn2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Fe

Iron
Cell ： PACKTEST Square Cup
Wavelength ： 510 nm, 540 nm

Color development： None → Light orange → Orange
Method： Reduction and o -Phenanthroline
Range： 0.10 ー 5.00 mg/L(ppm)
Reagent： WAK-Fe Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【Fe】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized iron (Fe2+, Fe3+: dissolved iron) in the sample is measured. The dissolved state ofiron greatly varies depending on the pH of the sample, and iron could exist in the form of suspended solid or precipitate. Performmeasurement after pretreatment according to the measurement purpose.
2. The optimum pH during color development is 6. If this pH cannot be achieved, neutralize the sample as necessary. A sample with asmall buffering capacity can be measured even if its pH is around 2.
3. To measure the concentration of the total iron in tap water or the like, you add 0.1 〜 0.2 mL of 1mol/L dilute sulfuric acid to
20 mL of sample, heat the sample until it almost boils, and suck the sample into the tube after cooling it down. It is possible tomeasure it without neutralization.
4. The concentration of such EDTA iron as used in hydroponics can also be measured without the pretreatment.
5. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:≤ 10mg/L,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Na+ , NH4+ , NO2 − , NO3 − ,PO43 − , SO42 − , Phenol≤ 10mg/L,: Anionic Surfactant , Residual Chlorine
Heavy metal ions:≤ 10mg/L,: Ba2+ , Co2+ , Cr3+ , Cr（Ⅵ）, Cu2+ , Mn2+ , Mo（Ⅵ）, Ni2+ , Zn2+
≤ 1mg/L,: Al3+ , CN −Seawater does not affect the measurement.
Oxidizing substance may affect the measurement.

－ 42 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Fe-D

Iron (Low Range)
Cell ： PACKTEST Square Cup
Wavelength ： 535 nm, 460 nm

Color development： None → Light red → Red
Method： Reduction and Bathophenatholine
Range： 0.05 ー 2.00 mg/L(ppm)
Reagent： WAK-Fe（D） Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【Fe-D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized iron (Fe2+, Fe3+: dissolved iron) in the sample is measured. The dissolved state ofiron greatly varies depending on the pH of the sample, and iron could exist in the form of suspended solid or precipitate. Performmeasurement after pretreatment according to the measurement purpose.
2. The optimum pH during color development is 7. If this pH cannot be achieved, neutralize the sample as necessary.
		 A sample with a small buffering capacity can be measured even if its pH is around 2.
3. To measure the concentration of the total iron in tap water or the like, you add 0.1 〜 0.2 mL of 1mol/L dilute sulfuric acid to
20 mL of sample, heat the sample until it almost boils, and suck the sample into the tube after cooling it down. It is possible tomeasure it without neutralization.
4. The concentration of such EDTA iron as used in hydroponics can also be measured without the pretreatment.
5. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
It is not possible to measure seawater.
Oxidizing substance may affect the measurement.

－ 43 －≤ 500mg/L,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Mn2+ , Na+ , NH 4+ ,NO3 − , SO 42 −≤ 500mg/L,: Phenol≤ 50mg/L,: Cr（Ⅵ）, Mo（Ⅵ）, Ni2+ , NO2 −≤ 10mg/L,: Zn2+
≤ 5mg/L,: PO 43 −≤ 2mg/L,: Cr 3+ , Residual Chlorine≤ 1mg/L,: Ba+ , CN −< 1mg/L,: Al3+ , Co2+ , Cu2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Fe 2 ＋

Iron (Divalent)
Cell ： PACKTEST Square Cup
Wavelength ： 510 nm, 540 nm

Color development： None → Light orange → Orange
Method：o -Phenanthroline
Range： 0.10 ー 5.00 mg/L(ppm)
Reagent： WAK-Fe2+ Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【Fe 2+ 】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized divalent iron (Fe2+) in the sample is measured.
2. The dissolved state of iron greatly varies depending on the pH of the sample, and iron could exist in the form of suspended solid or
precipitate.
		 To measure the concentration of total iron in tap water or the like, refer to "Fe Iron" or "Fe-D Iron (Low Range)".
3. The optimum pH during color development is 5. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:≤ 100mg/L,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Na+ , NH4+ , NO2 − , NO3−, PO43 − , SO42 − , Phenol≤ 10mg/L,: Anionic Surfactant< 1mg/L,: Residual Chlorine
Heavy metal ions:

Seawater does not affect the measurement.
Oxidizing substance (residual chlorine, Cr6+ etc.) turns Fe2+ into
Fe3+.

－ 44 －≤ 10mg/L,:	Al3+ , Ba2+ , Cr3+ , Fe3+ , Mn2+ , Mo（Ⅵ）, Ni2+ , Zn2+
≤ 1mg/L,: CN − , Co2+ , Cu2+
< 1mg/L,: Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Fe 2 ＋ -D

Iron (Divalent) (Low Range)
Cell ： PACKTEST Square Cup
Wavelength ： 535 nm, 460 nm

Color development： None → Light red → Red
Method： Bathophenatholine
Range： 0.05 ー 2.00 mg/L(ppm)
Reagent： WAK-Fe2+（D） Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【Fe 2+ -D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized divalent iron (Fe2+) in the sample is measured.
2. The dissolved state of iron greatly varies depending on the pH of the sample, and iron could exist in the form of suspended solid or
precipitate.
		 To measure the concentration of total iron in tap water or the like, refer to "Fe Iron" or "Fe-D Iron (Low Range)".
3. The optimum pH during color development is 5. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:≤ 1000mg/L,:	B（Ⅲ）, Cl − , F − , I − , K+ , Mg2+ , Na+ , NH4+ , NO2 − , NO3 − ,PO43 − , SO42 − , Phenol≤ 500mg/L,: Ca2+
≤ 50mg/L,: Anionic Surfactant< 1mg/L,: Residual Chlorine
Heavy metal ions:

It is not possible to measure seawater.
Oxidizing substance (residual chlorine, Cr6+ etc.) turns Fe2+ into
Fe3+.

－ 45 －≤ 10mg/L,: Al3+ , Ba2+ , Cr 3+ , Fe3+ , Mn2+ , Mo（Ⅵ）, Ni2+
≤ 5mg/L,: Zn2+
≤ 1mg/L,: Co2+ , Cu2+
< 1mg/L,: CN − , Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Fe 3 ＋

Iron（Trivalent）
Cell ： PACKTEST Square Cup
Wavelength ： 505 nm, 620 nm

Color development： None → Light red → Reddish brown
Method： Sulfosalicylic acidRange
： 1.0 ー 50.0 mg/L(ppm)
Reagent： WAK-Fe3+ Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【Fe 3+ 】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 1 minute have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized trivalent iron (Fe3+) in the sample is measured.
2. The dissolved state of iron greatly varies depending on the pH of the sample, and iron could exist in the form of suspended solid or
precipitate.
		 To measure the concentration of total iron in tap water or the like, refer to "Fe Iron" or "Fe-D Iron (Low Range)".
3. The optimum pH during color development is 1. If the pH of the sample is not within the range from 1 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/L,:	A g+ , Al3+ , B（Ⅲ）, Ba2+ , Ca2+ , Cl − , Co2+ , Cr 3+ , Fe2+ , K+ ,Mg2+ , Mn2+ , Mo（Ⅵ）, Na+ , NH 4+ , Ni2+ , NO3 − , PO 43 − ,SO 42 − , Zn2+ , Residual Chlorine, Silica , Phenol≤ 500mg/L,: Cr（Ⅵ）, Cu2+ , I − , Anionic Surfactant≤ 200mg/L,: NO2 − , V（Ⅴ）≤ 50mg/L,: F −≤ 10mg/L,: Cationic Surfactant≤ 1mg/L,: CN −It is not possible to measure seawater.
A reductive substance (ascorbic acid, etc.) turns Fe3+ into Fe2+.

－ 46 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

FOR

Formaldehyde
Cell ： PACKTEST Square Cup
Wavelength ： 660 nm

Color development： Yellow → Yellow green → Green
Method： MBTHRange： 0.20 ー 1.00 mg/L(ppm)
Reagent： WAK-FOR K-1（Small Pack）, Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【FOR】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add the K-1 reagent, attach the cap, shake the Cell 5 to 6 times todissolve the reagent, and leave the Cell for 3 minutes. （Fig.3）.

6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.4）

6WDUW
Fig.3

Fig.4

7. Lightly shake the tube in Step 6 about 10 times. （Fig.5）
8. Gently return the solution in the tube to the Cell, set it again in thecell box. （Fig.6）
9. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

Fig.6

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 5 to 8, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 10℃・・・×1.30
30℃・・・×0.60

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:≤ 100mg/L,:	B（Ⅲ）, Ca2+ , Cl − , F − , K+ , Mg2+ , Na+ , NH 4+ , NO3 − ,PO 43 −
≤ 50mg/L,: I − , SO 42 − , Phenol≤ 20mg/L,: Anionic Surfactant , Residual Chlorine≤ 1mg/L,: NO2 −Heavy metal ions:

It is not possible to measure seawater.
Oxidizing substance or reductive substance may affect themeasurement.

－ 47 －≤ 10mg/L,: Al3+ , Ba2+ , Co2+ , Cu2+ , Fe2+ , Fe3+ , Mn2+ , Ni2+ , Zn2+
≤ 5mg/L,: Cr（Ⅵ）≤ 1mg/L,: CN −Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

GLU

GlucoseCell ： PACKTEST Square Cup
Wavelength ： 539 nm, 610 nm

Color development： Light yellow → Light purple → Purple
Method： 4-Aminoantipyrine with enzymeRange： 0.5 ー 20.0 mg/L(ppm)
Reagent： WAK-GLU Tube
Reaction time ： 12 minutes after drawing sample into the tube.

Procedure
1. Press【GLU】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 30 times.（Fig.4）

6WDUW

7. Return the solution in the tube to the Cell in a gentle manner,
set it again in the cell box.（Fig.5）

Fig.3

Fig.4

8. After 12 minutes have elapsed, the concentration will be
automatically displayed.

Fig.5

CAUTION
1. The optimum pH during color development is 7. If the pH of the sample is not within the range from 6 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 20 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
It is not possible to measure seawater.
Oxidizing substances such as residual chlorine and hydrogen
peroxide cause a positive error.
Reductive substances sometimes cause a negative
measurement error.

－ 48 －≤ 1000mg/L,:	B（Ⅲ）, Cl − , F − , I − , K+, Mg2+, Na+, NH4+, NO3 − , PO43 − ,SO42 − , Citric acid, Succinic acid, Tartaric acid,
Fructose, Sucrose, Lactose≤ 500mg/L,: Mo（Ⅵ）, NO2 − , Zn2+, Silica≤ 200mg/L,: Mn2+, Ni2+, Starch≤ 100mg/L,: Ba2+, Co2+, Cr 3+, Phenol≤ 50mg/L,: Ca2+, Cr（Ⅵ）, Anionic Surfactant≤ 20mg/L,: Al3+, CN − , Galactose≤ 10mg/L,: Ag+ , Cu2+, Mannose, Cationic Surfactant≤ 5mg/L,: Fe3+
≤ 1mg/L,: Residual Chlorine< 1mg/L,: Fe2+, MaltoseⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

H 2O 2-C

Hydrogen Peroxide (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 470 nm, 550 nm

Color development： None → Yellow → Orange → Red brown
Method： Potassium Iodide
Range： 1 ー 200 mg/L(ppm)
Reagent： WAK-H2O2（C） Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【H 2 O 2 -C】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 1 minute has elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. The optimum pH during color development is 4. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.

Seawater does not affect the measurement.
Reductive substances such as F e 2 + and N O 2 － consumehydrogen peroxide.
NO 2 － may serve as an oxidizer and may cause a positive
measurement error.
Oxidizing substances such as residual chlorine and ozone cause
a positive measurement error.

－ 49 －≤ 1000mg/L,:	Al3+ , B（Ⅲ）, Ca2+ , Cl − , F − , K+ , Mg2+ , Mn2+ , Mo（Ⅵ）,
Na+ , NH4+ , Ni2+ , NO3 − , PO43 − , SO42 − , Phenol≤ 100mg/L,: Ba2+
≤ 50mg/L,: Cr（Ⅵ）, Zn2+ , Anionic Surfactant≤ 5mg/L,: Cu2+ , Fe3+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

H 2O 2

Hydrogen Peroxide
Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 590 nm

Color development： None → Light purple → Purple
Method： 4-Aminoantipyrine with enzyme
Range： 0.10 ー 2.50 mg/L(ppm)
Reagent： WAK-H2O2 Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【H 2 O 2 】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. The optimum pH during color development is 7. If the pH of the sample is not within the range from 6 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. If the concentration of hydrogen peroxide in the sample is 25 mg/L or less, the result is displayed as "OVER". However, note that
if the concentration is 50 mg/L or higher, the color development becomes pale, and a result may be obtained from a sample
exceeding the measurement range.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Reductive substances such as Fe 2+ and NO 2 － consumehydrogen peroxide.
Oxidizing substances such as residual chlorine and ozone cause
a positive measurement error.

－ 50 －≤ 1000mg/L,:	Ag+ , B（Ⅲ）, Ba2+ , Ca2+ , Cl − , I − , K+ , Mg2+ , Na+ , NH4+ ,Ni2+ , NO3 − , PO43 − , SO42 − , Zn2+
≤ 500mg/L,: F − , Fe3+ , NO2 −≤ 250mg/L,: Phenol≤ 50mg/L,: Cr3+ , Cr（Ⅵ）, Cu2+ , Anionic Surfactant≤ 20mg/L,: Al3+ , Co2+ , Mn2+
≤ 2mg/L,: Mo（Ⅵ）≤ 1mg/L,: CN −< 1mg/L,: Fe2+ , Residual ChlorineⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

HYD

Hydrazine
Cell ： PACKTEST Square Cup
Wavelength ： 455 nm, 480 nm

Color development： None → Yellow
Method：p -Dimethylaminobenzaldehyde
Range： 0.03 ー 1.00 mg/L(ppm)
Reagent： WAK-HYD K-1(Dropper), Tube
Reaction time ： 20 minutes after drawing sample into the tube.

Procedure
1. Press【HYD】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add two droplet of K-1 reagent, attach the cap, and shake the Cell 2
to 3 times. （Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.4）

.

6WDUW
Fig.3

Fig.4

Fig.5

Fig.6

7. Shake the tube in Step 6 by overturning it to right and left for 60
times in 1 minute. （Fig.5）
8. Gently return the solution in the tube to the Cell, set it again in thecell box. （Fig.6）
9. After 20 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. The optimum pH during color development is ≤2. Neutralize a sample of pH9 or greater with dilute sulfuric acid.
2. Perform measurement withe the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.

－ 51 －≤ 1000mg/L,:	Al3+ , B（Ⅲ）, Ca2+ , Cl − , Co2+ , Cu2+ , F − , Fe2+ , I − , K+ ,Mg2+ , Mn2+ , Na+ , NH4+ , Ni2+ , NO3 − , PO43 − , SO42 − ,Zn2+ , Silica , Phenol≤ 500mg/L ,: CN − , Mo（Ⅵ）, SO32 −≤ 200mg/L ,: Cr 3+≤ 100mg/L ,: Anionic Surfactant≤ 50mg/L ,: Fe3+
≤ 5mg/L ,: Ba2+
≤ 1mg/L ,: V（Ⅴ）< 1mg/L,: Cr（Ⅵ）, NO2 − , Residual ChlorineⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

KMnO 4

Potasium Permanganate Consumption

Cell ： PACKTEST Square Cup
Color development： Red purple → Green
Wavelength ： 525 nm
Method： Oxidation by potassium permanganate in alkaline
Range： 2.0 ー 10.0 mg/L(ppm)
Reagent： LR-COD-B-2 No.44 R-1（Liquid）, R-2（Liquid）, Neutralizer（Dropper）
Reaction time ： 10 minutes after R-2 reagent is added.

Procedure
1. Press【KMnO4】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line) and fill the Round
Cell with the sample for 25 mL (up to white line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】. Discard the sample in
the Cell.（Fig.2）

Fig.1

Fig.2

5. Add 0.5 mL of R-1 reagent into the Round Cell using the supplied
syringe, tightly attach the cap, and stir the solution 5 to 6 times.（Fig.3）
6. Add 1mL of R-2 reagent into the Round Cell using the supplied pipette,and press【Start】.（Fig.4）
7. Tightly attach the cap and stir the solution 5 to 6 times.（Fig.5）

5

5

6WDUW
Fig.3

Fig.4

Fig.5

Fig.6

8. Within 10 minutes, pour 1.5 mL of the solution in the Round Cell into
the Cell that has gone through zero adjustment, and put the Cell in
the cell box. (At this point, clean the Cell with the solution containedin the Round Cell.)（Fig.6）
9. After 10 minutes have elapsed, the concentration will be automaticallydisplayed.
10. Dispose of the wastewater in the Round Cell as of after measurementby adding about 8 droplets (0.5 mL) of neutralizer to it and confirmingthat it has become approximately neutral.

CAUTION
1. The optimum pH during color development is 12 or more. To an acid sample, add dilute sodium hydroxide solution or the like so as
to adjust the pH of the sample to 6 or more.
2. Perform measurement with the sample temperature set to 15 to 25℃ .
3. Before filling the Cell with the solution in the measurement procedure Steps 8, rinse the Cup with the solution 2 to 3 times.
4. It is not possible to measure seawater.

Relationship with official method
While the measurement method of of potassium permanganate consumption is stipulated in Standard Methods for the Examinationof Water (Japan Water Works Association) or other regulations, the measurement method we offer applies the alkali method (CODOH)
according to JIS K 0102 19 and allows simple measurement in a short period of time.
In the Standard Methods for the Examination of Water, the amount of potassium permanganate that has been consumed in a boiled
water bath for 5 minutes under the acid condition is obtained through titration. On the other hand, the measurement method we offer
obtains the amount of potassium permanganate that has been consumed at a room temperature for 10 minutes under the alkali
condition from the decrease amount of absorbance.
Verification is conducted by using glucose reference solution, but the degree of oxidation of oxidized substance in the sample bypotassium permanganate differs depending on the type and the amount of the substance.
As the values obtained by this measurement method are only approximate values and may not coincide with values obtained by theStandard Methods for the Examination of Water, use this method after obtaining the relationship between those methods.

－ 52 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

MAL

M-Alkalinity

Color development： Yellow → Green → Blue-green
Method： Absorptiometry with pH Indicator for Buffering Capacity
Range： 20 ー 80 mg/L(ppm)
Reagent： WAK-MAL Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 470 nm, 560 nm

Procedure
1. Press【MAL】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Shake the tube in Step 5 about 15 times to dissolve the reagent in
the tube completely. （Fig.4）

6WDUW
Fig.3

Dissolve the reagent
completely

Fig.4

7. Gently return the solution in the tube to the Cell, set it again in thecell box. （Fig.5）
8. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, M-alkalinity (Total alkalinity: OH － , HCO3－ , CO32－ , etc.) in the sample is measured.
2. The measurement value is the concentration as calcium carbonate (mg/L as CaCO3).
		 The obtained result can be converted into the equivalent concentration (normality, Unit: meq/L) by multiplying it by 0.020.
		 M-alkalinity of the typical natural water is derived from HCO3－（hydrogencarbonate, bicarbonate）. In order to convert the obtained
result into the concentration of HCO3－ , multiply it by 1.22.
3. The measurement result may be affected by unclean conditions. Clean up your hands before the measurement.
4. From the definition, M-alkalinity of the sample which is pH below 4.8 is zero. If the sample contains large amount of acid, the colorof the solution in the tube may turn to dark yellow or orange.
5. Perform measurement with the sample temperature set to 15 to 30 ℃ .
6. Larger or smaller sample volume will imply higher or lower value, respectively.
		 Use of a measuring pipette or the like to measure the volume of the sample (1.5mL) enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptablelevel by adding each of the single substances to the standard
solution.
Seawater does not affect the measurement, but it may
contain alkalinity more than measurement range.
Less than 10% of ethanol does not affect the measurement.
In this method, alkalinity which is derived from phosphate(HPO42 －、PO43 － ), borate（BO2 －、B4O72 － , etc.）and ammonia(NH 3) is measured, but phosphate which is more than 200
mg/L as PO43 － causes negative error.

－ 53 －≤ 1000mg/ L ,:	H3BO3（Boric acid）、Ba2+、Br −、Ca2+、Cl −、I −、K+、
Mg2+、Mn2+、Na+、NH 4+、N2H5+（Hydrazinium）、NO3 −、SO 42 −、Glucose、Phenol≤ 200mg/L,: H2PO 4 −≤ 50mg/L,: Anionic Surfactant≤ 20mg/L,: Nonionic surfactant≤ 10mg/L,: F −、NO2 −≤ 5mg/L,: Residual Chlorine、Cationic SurfactantⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

PAL

P-Alkalinity

Color development： Yellow → Brown → Purple
Method： Absorptiometry with pH Indicator for Buffering Capacity
Range： 100 ー 600 mg/L(ppm)
Reagent： WAK-PAL Tube
Reaction time ： 1 minute after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 488 nm, 623 nm

Procedure
1. Press【PAL】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Shake the tube in Step 5 about 15 times to dissolve the reagent in
the tube completely. （Fig.4）

6WDUW
Fig.3

Dissolve the reagent
completely

Fig.4

7. Gently return the solution in the tube to the Cell, set it again in thecell box. （Fig.5）
8. After 1 minute has elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, P-alkalinity (Phenolphthalein alkalinity: OH － , CO32－ , etc.) in the sample is measured.
2. The measurement value is the concentration as calcium carbonate (mg/L as CaCO3).
		 The obtained result can be converted into the equivalent concentration (normality, Unit: meq/L) by multiplying it by 0.020.
3. The measurement result may be affected by unclean conditions. Clean up your hands before the measurement.
4. From the definition, P-alkalinity of the sample which is pH below 8.3 is zero. If the sample shows highly acidic pH, the color of thesolution in the tube may turn to red or orange.
5. Perform measurement with the sample temperature set to 15 to 30 ℃ .
6. Larger or smaller sample volume will imply higher or lower value, respectively.
		 Use of a measuring pipette or the like to measure the volume of the sample (1.5mL) enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptablelevel by adding each of the single substances to the standard
solution.

≤ 1000mg/ L ,:	Ba2+、Br −、Ca2+、Cl −、HCO3 −、F −、I −、K+、Mg2+、
Na+、NO2 −、NO3 −、HPO42 −、SO42 −、Glucose、Phenol≤ 500mg/L,: Nonionic Surfactant≤ 50mg/L,: Anionic Surfactant≤ 20mg/L,: Cationic Surfactant≤ 1mg/L,: Residual Chlorine

Seawater does not affect the measurement.
Less than 5% of ethanol does not affect the measurement.

－ 54 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,CorpMn

Manganese
Cell ： PACKTEST Square Cup
Wavelength ： 537 nm

Color development： None → Light red → Red
Method： Potassium Periodate
Range： 0.5 ー 20.0 mg/L(ppm)
Reagent： WAK-Mn Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【Mn】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized divalent to hexavalent manganese in the sample is measured.
		 If result of manganese concentration including suspension and precipitate is required, dissolve manganese in advance and thenperform measurement.
2. To measure the concentration of manganese, including septivalent manganese ions (red), conduct reduction in advance and thenperform measurement. Or perform zero adjustment by using a sample whose red color is decolored by adding a large amount of
reducing agent, discard the sample and thoroughly clean the Cell, take the sample again, and perform measurement as usual bysucking the sample into the tube.
3. The optimum pH during color development is 7. If the pH of the sample is not within the range from 5 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level≤ 1000mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , K+ , Mg2+ , Mo（Ⅵ）, Na+ , NH 4+ ,by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
The existence of a large amount of reductive substance causes
a negative measurement error.
For example, in the case of sodium hydrogen sulfite, its
coexistence at 10 g/L or more affects the measurement value.

－ 55 －

NO3 − , PO 43 − , SO 42 − , Anionic Surfactant ,Residual Chlorine , Formaldehyde≤ 500mg/L,:	Ni2+≤ 200mg/L,:	Al3+ , Ba2+ , Zn2+
≤ 100mg/L,:	Cu2+ , Fe2+ , Fe3+
≤ 50mg/L,: Cr（Ⅵ）, NO2 −≤ 20mg/L,: CN − , Co2+
≤ 5mg/L,: Cr 3+ , I − , PhenolⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

Mo

Molybdenum
Cell ： PACKTEST Square Cup
Wavelength ： 555 nm, 670 nm

Color development： None → Yellow → Brown → Red
Method： Modified CatecholRange： 5 ー 150 mg/L(ppm)
Reagent： WAK-Mo Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【Mo】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of molybdenum(VI) in molybdate ion（MoO42－）state is measured and is displayed as a converted
value of molybdenum (Mo).
		 If result of molybdenum concentration including suspension and precipitate is required, dissolve molybdenum in advance and thenperform measurement.
2. It is not possible to measure the concentration of molybdenum sulfide.
3. The obtained result can be converted into the concentration of molybdate ion（MoO42－）by multiplying it by 1.67.
4. The optimum pH during color development is 7. If the pH of sample is not within the range from 4 to 9, neutralize the sample withdilute sulfuric acid or dilute sodium hydroxide solution, etc.
5. Perform measurement withe the sample temperature set to 15 to 30℃

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	Ba2+, Ca2+, Cl－, F－, I－, K+, Mg2+, Mn2+, Na+, NH4+, NO2－, NO3－,
SO32 － , SO42 － , Zn2+, Anionic Surfactant , Hydrazine , Phenol≤ 500mg/L,: CN － , Co2+ , Residual Chlorine≤ 200mg/L,: B（Ⅲ）, Cr 3+ , Cr（Ⅵ）, Ni2+
≤ 20mg/L,: Cu2+
≤ 10mg/L,: Fe2+ , Fe3+
≤ 2mg/L,: Al3+ ,< 1mg/L,: V（Ⅴ）

Seawater does not affect the measurement.

－ 56 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Ni-D

Nickel (DPM)
Cell ： PACKTEST Square Cup
Wavelength ： 550 nm, 535 nm, 670 nm

Color development： None → Light Pink → Pink
Method： Nioxime
Range： 0.3 ー 10.0 mg/L(ppm)
Reagent： WAK-Ni（D） Tube
Reaction time ： 2 minutes after drawing sample into the tube.

Procedure
1. Press【Ni-D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Shake the tube in Step 5 by overturning it to right and left for 60
times in 1 minute. （Fig.4）

6WDUW
Fig.3

Fig.4

7. Return the solution in the tube to the Cell in a gentle manner, set it
again in the cell box. （Fig.5）
8. After 2 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of ionized nickel (Ni 2+ ) in the sample is measured. If a result including turbid, deposition, andcomplex is required, dissolve the target substance in advance and then perform measurement.
2. The optimum pH during color development is 4. If the pH of the sample is not within the range from 4 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.

－ 57 －≤ 1000mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , Cr3+ , F − , I − , K+ , Mg2+ , Mn2+ , Mo（Ⅵ）,
Na+ , NH4+ , NO2 − , NO3 − , PO43 − , SO42 − , Zn2+ , Phenol≤ 500mg/L ,:	A g+ , Residual Chlorine≤ 100mg/L ,:	Anionic Surfactant≤ 50mg/L ,: Ba2+ , Cr（Ⅵ）≤ 20mg/L ,: Al3+ , Co2+
≤ 10mg/L ,: Fe3+
≤ 2mg/L ,: Fe2+
≤ 1mg/L ,: CN −< 1mg/L,: Cu2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,CorpNH 4Ammonium
Cell ： PACKTEST Square Cup
Wavelength ： 643 nm, 590 nm

Color development： None → Light blue → Blue
Method： Indophenol BlueRange
： 0.20 ー 5.00 mg/L(ppm)
Reagent： WAK-NH4-4 Tube
Reaction time ： 10 minutes after drawing sample into the tube.

Procedure
1. Press【NH4】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

5. Submerge the aperture of the tube in the sample, and release yourfinger to suck the whole amount of the sample at once. At the sametime, press【Start】.（Fig.3）

Fig.2

Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

6. Shake the tube in Step 5 by overturning it to right and left for 30
times in 30 seconds. （Fig.4）

6WDUW
Fig.3

Fig.4

7. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box. （Fig.5）
8. After 10 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. The optimum pH during color development is 13. If the pH of the sample is not within the range from 5 to 13, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. As the results tend to vary, note the following points to acquire accurate results.
		 ●	
To suck the sample into the tube, release your finger while depressing the aperture of the tube against the bottom of the Cell tosuck the whole amount of the sample at once.
		 ●	
Immediately after having sucked the sample into the tube, start shaking the tube.
		 ●	
Shake the tube by overturning it to right and left so that the sample moves all the way to both ends of the tube. Shake the tube
for 30 seconds (one reciprocation per second).
4. After the tube is shaken in Step 6 of "Procedure", immediately and gently return the solution in the tube to the Cell. If you take time
before returning the solution, turbid may be generated.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing it withthe official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:

It is not possible to measure seawater.
To measure seawater or a sample containing a large amount ofcoexistent substances, perform measurement after separating
ammonium ions by distillation because turbid or abnormal color
development causes an error.
To perform distillation according to the JIS K 0102 42.1
distillation method.

Heavy metal ions:≤ 1000mg/ L ,:	B（Ⅲ）, Cl − , F − , I − , K+ , Na+ , NO3 − , SO 42 − ,Anionic Surfactant , Residual Chlorine≤ 500mg/L,: Phenol≤ 250mg/L,: PO 43 −≤ 100mg/L,:	Ca2+≤ 50mg/L,: Mg2+ , NO2 −≤ 5mg/L,: Formaldehyde－ 58 －≤ 250mg/L,: Zn2+
≤ 100mg/L,:	Ba2+ , Cr（Ⅵ）, Ni2+
≤ 50mg/L,: Al3+
≤ 25mg/L,: Cr 3+ , Mo（Ⅵ）≤ 10mg/L,: Ag+ , Cu2+
≤ 5mg/L,: Mn2+
≤ 1mg/L,: Co2+
< 1mg/L,: Fe2+ , Fe3+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NH 4-N

Ammonium-Nitrogen
Cell ： PACKTEST Square Cup
Wavelength ： 643 nm, 590 nm

Color development： None → Light blue → Blue
Method： Indophenol BlueRange
： 0.20 ー 4.00 mg/L(ppm)
Reagent： WAK-NH4-4 Tube
Reaction time ： 10 minutes after drawing sample into the tube.

Procedure
1. Press【NH 4 -N】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

5. Submerge the aperture of the tube in the sample, and release yourfinger to suck the whole amount of the sample at once. At the sametime, press【Start】.（Fig.3）

Fig.2

Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

6. Shake the tube in Step 5 by overturning it to right and left for 30
times in 30 seconds. （Fig.4）

6WDUW
Fig.3

Fig.4

7. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box. （Fig.5）
8. After 10 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. The optimum pH during color development is 13. If the pH of the sample is not within the range from 5 to 13, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. As the results tend to vary, note the following points to acquire accurate results.
		 ●	
To suck the sample into the tube, release your finger while depressing the aperture of the tube against the bottom of the Cell tosuck the whole amount of the sample at once.
		 ●	
Immediately after having sucked the sample into the tube, start shaking the tube.
		 ●	
Shake the tube by overturning it to right and left so that the sample moves all the way to both ends of the tube. Shake the tube
for 30 seconds (one reciprocation per second).
4. After the tube is shaken in Step 6 of "Procedure", immediately and gently return the solution in the tube to the Cell. If you take time
before returning the solution, turbid may be generated.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

Except for Heavy metal ions:

It is not possible to measure seawater.
To measure seawater or a sample containing a large amount ofcoexistent substances, perform measurement after separating
ammonium ions by distillation because turbid or abnormal color
development causes an error.
To perform distillation according to the JIS K 0102 42.1
distillation method.

Heavy metal ions:≤ 1000mg/ L ,:	B（Ⅲ）, Cl − , F − , I − , K+ , Na+ , NO3 − , SO 42 − ,Anionic Surfactant , Residual Chlorine≤ 500mg/L,: Phenol≤ 250mg/L,: PO 43 −≤ 100mg/L,:	Ca2+≤ 50mg/L,: Mg2+ , NO2 −≤ 5mg/L,: Formaldehyde－ 59 －≤ 250mg/L,: Zn2+
≤ 100mg/L,:	Ba2+ , Cr（Ⅵ）, Ni2+
≤ 50mg/L,: Al3+
≤ 25mg/L,: Cr 3+ , Mo（Ⅵ）≤ 10mg/L,: Ag+ , Cu2+
≤ 5mg/L,: Mn2+
≤ 1mg/L,: Co2+
< 1mg/L,: Fe2+ , Fe3+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NH 4-DAmmonium (Low Range)

Cell ： PACKTEST Square Cup
Color development： None → Light blue → Blue
Wavelength ： 637 nm, 590 nm
Method： Distillation and Indophenol BlueRange
： 0.05 ー 2.00 mg/L(ppm)
Reagent： LR-NH4-A-2 No.17A R-1（Liquid）, R-2（Small Pack）, R-3（Liquid）
Reaction time ： 5 minutes after R-3 reagent is added.
Other Items to Use ： Water Analysis Set: Ammonium (Low Range) (Model: WA-NH4(L)-2)
Preparation Procedure： Read the instruction for pretreatment reagent (WA-NH4-DR).

Procedure
1. Press【NH 4 -D】.
2. Press【OK】to switch to the photometry window.
3. Adjust the volume of the solution captured through distillation to 25 mL
if the volume is less than 25 mL, and then fill the Round Cell with thewhole amount of the solution. Fill the Cell with pure water for 1.5 mL(up to line).（Fig.1）
4. Put the Cell in the cell box and press【Zero】. Take out the Cell anddiscard the pure water.（Fig.2）

Solution5

Pure water

=HUR

Fig.1

5. Add 3 mL of the R-1 reagent into the Round Cell using the supplied
pipette.（Fig.3）

Fig.2

5

6. Add the R-2 reagent to the Round Cell, tightly attach the cap,
immediately shake the Round Cell strongly for 10 seconds, and waitfor 3 minutes.（Fig.4）
7. Add 2 mL of R-3 reagent to the Round Cell using the supplied pipette,press【Start】, tightly attach the cap, and stir the solution 5 to 6
times.（Fig.5）
8. Within 5 minutes, pour the solution in the Round Cell for 1.5 mL into
the Cell that has gone through zero adjustment (up to line) and put
the Cell in the cell box.（Fig.6）

Fig.3

Fig.4

R-3
5

6WDUW
Fig.5

Fig.6

9. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. As the glass portion of the distiller becomes hot during distillation, be careful not to get burned.
2. Perform measurement with the solution temperature set to 20℃ .
		 If the solution temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 15℃・・・×1.2
25℃・・・×0.75
30℃・・・×0.65
		 If the temperature is 40℃ or higher, the color will abnormally develop in red.
3. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

－ 60 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NH 4-N-D

Ammonium-Nitrogen (Low Range)

Cell ： PACKTEST Square Cup
Color development： None → Light blue → Blue
Wavelength ： 637 nm, 590 nm
Method： Distillation and Indophenol BlueRange
： 0.05 ー 1.50 mg/L(ppm)
Reagent： LR-NH4-A-2 No.17A R-1（Liquid）, R-2（Small Pack）, R-3（Liquid）
Reaction time ： 5 minutes after R-3 reagent is added.
Other Items to Use ： Water Analysis Set: Ammonium (Low Range) (Model: WA-NH4(L)-2)
Preparation Procedure： Read the instruction for pretreatment reagent (WA-NH4-DR).

Procedure
1. Press【NH 4 -N-D】.
2. Press【OK】to switch to the photometry window.
3. Adjust the volume of the solution captured through distillation to 25 mL
if the volume is less than 25 mL, and then fill the Round Cell with thewhole amount of the solution. Fill the Cell with pure water for 1.5 mL(up to line).（Fig.1）
4. Put the Cell in the cell box and press【Zero】. Take out the Cell anddiscard the pure water.（Fig.2）

Solution5

Pure water

=HUR

Fig.1

5. Add 3 mL of the R-1 reagent into the Round Cell using the supplied
pipette.（Fig.3）

Fig.2

5

6. Add the R-2 reagent to the Round Cell, tightly attach the cap,
immediately shake the Round Cell strongly for 10 seconds, and waitfor 3 minutes.（Fig.4）
7. Add 2 mL of R-3 reagent to the Round Cell using the supplied pipette,press【Start】, tightly attach the cap, and stir the solution 5 to 6
times.（Fig.5）
8. Within 5 minutes, pour the solution in the Round Cell for 1.5 mL into
the Cell that has gone through zero adjustment (up to line) and put
the Cell in the cell box.（Fig.6）

Fig.3

Fig.4

R-3
5

6WDUW
Fig.5

Fig.6

9. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. As the glass portion of the distiller becomes hot during distillation, be careful not to get burned.
2. Perform measurement with the solution temperature set to 20℃ .
		 If the solution temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 15℃・・・×1.2
25℃・・・×0.75
30℃・・・×0.65
		 If the temperature is 40℃ or higher, the color will abnormally develop in red.
3. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

－ 61 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 2-C

Nitrite (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 560 nm

Color development： None → Light yellow → Reddish brown
Method： Griess RomijinRange： 3 ー 100 mg/L(ppm)
Reagent： WAK-NO2（C） Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【NO 2 -C】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, and then wait forreaction for about 4 minutes. （Fig.4）

6WDUW
Fig.3

5-6
Fig.4

7. Gently return the solution in the tube to the Cell when the time countdown reaches under 1 minute, set it again in the cell box. （Fig.5）
8. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. Note that in this PACKTEST, a large amount of bubbles will be generated. If bubbles are attached to the inner wall of the Cell,remove them as much as possible by, for example, snapping the Cell with your finger.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level
Except for Heavy metal ions:by adding each of the single substances to the standard solution.
2+
−
−
−
+
2+
+
−
3−
Seawater does not affect the measurement.
Generally, nitrite ions do not coexist with oxidizing substancessuch as residual chlorine, but if residual chlorine and chloramines
exist, they develop their color in red and may be mistaken as
nitrite even when nitrite ions do not exist.

≤ 100mg/ L ,:	B（Ⅲ）, Ca , Cl , F , I , K , Mg , Na , NO3 , PO4
SO 42 − , Anionic Surfactant , Phenol≤ 50mg/L,: NH 4+≤ 5mg/L,: Residual Chlorine,

Heavy metal ions:－ 62 －≤ 10mg/ L ,:	Al3+ , Ba2+ , CN − , Co2+ , Cr 3+ , Cu2+ , Fe2+ , Fe3+ , Mn2+ ,Mo（Ⅵ）, Ni2+ , Zn2+
≤ 1mg/L,: Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 2-N-C

Nitrite-Nitrogen (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 560 nm

Color development： None → Light yellow → Reddish brown
Method： Griess RomijinRange： 1.0 ー 30.0 mg/L(ppm)
Reagent： WAK-NO2（C） Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【NO 2 -N-C】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, and then wait forreaction for about 4 minutes. （Fig.4）

6WDUW
Fig.3

5-6
Fig.4

7. Gently return the solution in the tube to the Cell when the time countdown reaches under 1 minute, set it again in the cell box. （Fig.5）
8. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. Note that in this PACKTEST, a large amount of bubbles will be generated. If bubbles are attached to the inner wall of the Cell,remove them as much as possible by, for example, snapping the Cell with your finger.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level
Except for Heavy metal ions:by adding each of the single substances to the standard solution.
2+
−
−
−
+
2+
+
−
3−
Seawater does not affect the measurement.
Generally, nitrite ions do not coexist with oxidizing substancessuch as residual chlorine, but if residual chlorine and chloramines
exist, they develop their color in red and may be mistaken as
nitrite even when nitrite ions do not exist.

≤ 100mg/ L ,:	B（Ⅲ）, Ca , Cl , F , I , K , Mg , Na , NO3 , PO4
SO 42 − , Anionic Surfactant , Phenol≤ 50mg/L,: NH 4+≤ 5mg/L,: Residual Chlorine,

Heavy metal ions:－ 63 －≤ 10mg/L,:	Al3+ , Ba2+ , CN − , Co2+ , Cr 3+ , Cu2+ , Fe2+ , Fe3+ , Mn2+ ,Mo（Ⅵ）, Ni2+ , Zn2+
≤ 1mg/L,: Cr（Ⅵ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 2

NitriteCell ： PACKTEST Square Cup
Wavelength ： 539 nm, 570 nm

Color development： None → Light red → Red
Method： Naphthylethylenediamine
Range： 0.02 ー 1.00 mg/L(ppm)
Reagent： WAK-NO2 Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【NO 2 】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. As nitrite also exists in the air and may be dissolved in pure water, pay due attention during measurement at low concentration.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level≤ 1000mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Mn2+ , Na+ , NH4+ ,by adding each of the single substances to the standard solution.
NO3 − , PO43 − , SO42 − , Phenol
Seawater does not affect the measurement.
Generally, nitrite ions do not coexist with oxidizing substancessuch as residual chlorine, but if residual chlorine and chloramines
exist, they develop their color in red and may be mistaken as
nitrite even when nitrite ions do not exist.

－ 64 －≤ 500mg/L,: Co2+
≤ 250mg/L,: CN − , Cr 3+≤ 100mg/L,:	Cu2+ , Mo（Ⅵ）, Zn2+
≤ 50mg/L,: Ni2+
≤ 25mg/L,: Fe2+
≤ 10mg/L,: Al3+ , V（Ⅴ）≤ 2mg/L,: Cr（Ⅵ）, Fe3+
< 1mg/L,: Ag+ , Ba2+ , Residual ChlorineⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 2-N

Nitrite-Nitrogen
Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 570 nm

Color development： None → Light red → Red
Method： Naphthylethylenediamine
Range： 0.010 ー 0.300 mg/L(ppm)
Reagent： WAK-NO2 Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【NO 2 -N】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. As nitrite also exists in the air and may be dissolved in pure water, pay due attention during measurement at low concentration.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level≤ 1000mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Mn2+ , Na+ , NH4+ ,by adding each of the single substances to the standard solution.
NO3 − , PO43 − , SO42 − , Phenol
Seawater does not affect the measurement.
Generally, nitrite ions do not coexist with oxidizing substancessuch as residual chlorine, but if residual chlorine and chloramines
exist, they develop their color in red and may be mistaken as
nitrite even when nitrite ions do not exist.

－ 65 －≤ 500mg/L,: Co2+
≤ 250mg/L,: CN − , Cr 3+≤ 100mg/L,:	Cu2+ , Mo（Ⅵ）, Zn2+
≤ 50mg/L,: Ni2+
≤ 25mg/L,: Fe2+
≤ 10mg/L,: Al3+ , V（Ⅴ）≤ 2mg/L,: Cr（Ⅵ）, Fe3+
< 1mg/L,: Ag+ , Ba2+ , Residual ChlorineⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3-C

Nitrate (High Range)

In this analyte the procedure should be divided into 2 methods according to the sample state.
Be careful that each method uses their specified reagent.

1. NO 3-C_1

Nitrate (High Range) (NO2ー ≤ 1mg/L)

Range：200 ー 2000 mg/L (ppm)
Reagent ：WAK-NO3(C)Perform the regular Nitrate (High Range) measurement procedure.

2. NO 3-C_2

Nitrate (High Range) (NO2ー 1 ー 10mg/L)

Range：200 ー 2000 mg/L (ppm)
Reagent ：Pretreatment reagent (NO3-RA), WAK-NO3(C)It is necessary to remove Nitrite by pretreatment reagent before the regular Nitrate (High Range) measurement procedure.

－ 66 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3-C_1

Nitrate (High Range) (NO2ー ≤ 1mg/L)
Cell ： PACKTEST Square Cup
Wavelength ： 560 nm

Color development： None → Light yellow → Brown
Method： Reduction and Griess Romijin
Range： 200 ー 2000 mg/L(ppm)
Reagent： WAK-NO3（C） Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【NO 3 -C_1】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

=HUR

6WDUW

Fig.2

Fig.3

5. Submerge the aperture of the tube in the sample, and the release your finger to suckthe whole amount of the sample at once. At the same time, press【Start】.（Fig.3）
6. Shake the tube in Step 5 by overturning it to right and left for 60 times in 1 minute,and then wait for reaction for about 3 minutes. （Fig.4）

Fig.4

7. Gently return the solution in the tube to the Cell when the time count down reaches
under 1 minute, set it again in the cell box. （Fig.5）
8. After 5 minutes have elapsed, the concentration will be automatically displayed.
Fig.5

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 10℃・・・×0.80
30℃・・・×1.1
3. As the results tend to vary, note the following points to acquire accurate results.
		●		 To suck the sample into the tube, release your finger while depressing the aperture of the tube against the bottom of the Cellto suck the sample at once.
		●		 
Immediately after having sucked the sample into the tube, start shaking the tube.
		●		 
Shake the tube by overturning it to right and left so that the sample moves all the way to both ends of the tube. Shake thetube for 1 minute (one reciprocation per second).
		●		 
If the tube is shaken intensely, the measurement value will be low.
4. If nitrite ions coexist in the sample at a high concentration, as their color develops stronger than nitrate ions and greatly affects
the result, perform measurement by following the procedures for "NO3-C_2 Nitrate (High Range) (NO2ー 1 ー 10 mg/L)".
5. Note that in this PACKTEST, a large amount of bubbles will be generated. If bubbles are attached to the inner wall of the Cell,remove them as much as possible by, for example, snapping the Cell with your finger.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level Except for Heavy metal ions:by adding each of the single substances to the standard solution.
≤ 100mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Na+ , NH4+ , PO43 − ,Seawater does not affect the measurement.
Oxidizing substance or reductive substance may affect themeasurement.

SO 42 − , Phenol , Anionic Surfactant≤ 10mg/L,: Residual Chlorine

Heavy metal ions:－ 67 －≤ 10mg/ L ,:	Al3+ , Ba2+ , CN − , Cr 3+ , Mn2+ , Mo（Ⅵ）, Zn2+
≤ 1mg/L,: Cr（Ⅵ）, Fe3+ , Ni2+
< 1mg/L,: Co2+ , Cu2+ , Fe2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3-C_2

Nitrate (High Range) (NO 2ー 1 − 10mg/L)

Color development： None → Light yellow → Brown
Method： Reduction and Griess Romijin
Range： 200 ー 2000 mg/L(ppm)
Reagent： Pretreatment reagent (NO3-RA)（Pack）, WAK-NO3（C） Tube
Other Items to Use ： Beaker, Heater
Reaction time ： 5 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 560 nm

Pretreatment method
First, remove the nitrite ions mixed in the sample by using pretreatment
reagent.

NO3-RA

1. Fill a beaker with 30 mL of sample, add pretreatment reagent (NO3-RA)in the sample, and stir the sample 5 to 6 times.（Fig.1）
2. Heat the sample, boil it for 2 minutes, and cool it down to 15 to 30℃ .
（Fig.2）
		 If the amount of the sample has decreased after cooling down, add
pure water up to 30 mL.

Fig.1

Fig.2

3. Fill the Cell with the pretreated sample for 1.5 mL (up to line). （Fig.3）
Fig.3

Procedure
1. Press【NO 3 -C_2】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Put the Cell filled with the pretreated sample in the cell box and press【Zero】.（Fig.4）

=HUR

6WDUW

Fig.4

Fig.5

4. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.5）
5. Shake the tube in Step 4 by overturning it to right and left for 60
times in 1 minute, and then wait for reaction for about 3 minutes.
（Fig.6）Fig.6

6. Gently return the solution in the tube to the Cell when the time countdown reaches under 1 minute, set it again in the cell box. （Fig.7）
7. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.7

CAUTION
Refer to "NO3-C_1 Nitrate (High Range) (NO2ー≤1mg/L)".

－ 68 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3-N-C Nitrate-Nitrogen (High Range)
In this analyte the procedure should be divided into 2 methods according to the sample state.
Be careful that each method uses their specified reagent.

1. NO3-N-C1 Nitrate-Nitrogen (High Range) (NO2ー -N ≤ 0.3mg/L)
Range：45 ー 450 mg/L (ppm)
Reagent ：WAK-NO3(C)Perform the regular Nitrate-Nitrogen (High Range) measurement procedure.

2. NO3-N-C2

Nitrate-Nitrogen (High Range) (NO2ー -N 0.3 ー 3mg/L)

Range：45 ー 450 mg/L (ppm)
Reagent ：Pretreatment Reagent (NO3-RA), WAK-NO3(C)It is necessary to remove Nitrite by pretreatment reagent before the regular Nitrate-Nitrogen (High Range) measurement procedure.

－ 69 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO3-N-C1 Nitrate-Nitrogen (High Range) (NO2ー -N ≤ 0.3mg/L)
Cell ： PACKTEST Square Cup
Wavelength ： 560 nm

Color development： None → Light yellow → Brown
Method： Reduction and Griess Romijin
Range： 45 ー 450 mg/L(ppm)
Reagent： WAK-NO3（C） Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【NO 3 -N-C1】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

=HUR

6WDUW

Fig.2

Fig.3

5. Submerge the aperture of the tube in the sample, and the release your finger to suckthe whole amount of the sample at once. At the same time, press【Start】.（Fig.3）
6. Shake the tube in Step 5 by overturning it to right and left for 60 times in 1 minute,and then wait for reaction for about 3 minutes. （Fig.4）

Fig.4

7. Gently return the solution in the tube to the Cell when the time count down reaches
under 1 minute, set it again in the cell box. （Fig.5）
8. After 5 minutes have elapsed, the concentration will be automatically displayed.
Fig.5

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 10℃・・・×0.80
30℃・・・×1.1
3. As the results tend to vary, note the following points to acquire accurate results.
		●		 To suck the sample into the tube, release your finger while depressing the aperture of the tube against the bottom of the Cellto suck the sample at once.
		●		 
Immediately after having sucked the sample into the tube, start shaking the tube.
		●		 
Shake the tube by overturning it to right and left so that the sample moves all the way to both ends of the tube. Shake thetube for 1 minute (one reciprocation per second).
		●		 
If the tube is shaken intensely, the measurement value will be low.
4. If nitrite ions coexist in the sample at a high concentration, as their color develops stronger than nitrate ions and greatly affects
the result, perform measurement by following the procedures for "NO3-N-C2 Nitrate-Nitrogen (NO2ー -N 0.3 ー 3 mg/L)".
5. Note that in this PACKTEST, a large amount of bubbles will be generated. If bubbles are attached to the inner wall of the Cell,remove them as much as possible by, for example, snapping the Cell with your finger.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level Except for Heavy metal ions:by adding each of the single substances to the standard solution.
≤ 100mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Na+ , NH4+ , PO43 − ,Seawater does not affect the measurement.
Oxidizing substance or reductive substance may affect themeasurement.

SO 42 − , Phenol , Anionic Surfactant≤ 10mg/L,: Residual Chlorine

Heavy metal ions:－ 70 －≤ 10mg/ L ,:	Al3+ , Ba2+ , CN − , Cr3+ , Mn2+ , Mo（Ⅵ）, Zn2+
≤ 1mg/L,: Cr（Ⅵ）, Fe3+ , Ni2+
< 1mg/L,: Co2+ , Cu2+ , Fe2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO3-N-C2

Nitrate-Nitrogen (High Range) (NO2ー -N 0.3 ー 3mg/L)

Color development： None → Light yellow → Brown
Method： Reduction and Griess Romijin
Range： 45 ー 450 mg/L(ppm)
Reagent： Pretreatment reagent（NO3-RA）（Pack）, WAK-NO3（C） Tube
Other Items to Use ： Beaker, Heater
Reaction time ： 5 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 560 nm

Pretreatment method
First, remove the nitrite ions mixed in the sample by using pretreatment reagent.
1. Fill a beaker with 30 mL of sample, add pretreatment reagent (NO3-RA)in the sample, and stir the sample 5 to 6 times.（Fig.1）
2. Heat the sample, boil it for 2 minutes, and cool it down to 15 to 30℃ .
（Fig.2）
		 If the amount of the sample has decreased after cooling down, add
pure water up to 30 mL.

NO3-RA

Fig.1

Fig.2

3. Fill the Cell with the pretreated sample for 1.5 mL (up to line). （Fig.3）
Fig.3

Procedure
1. Press【NO 3 -N-C2】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Put the Cell filled with the pretreated sample in the cell box and press【Zero】.（Fig.4）

=HUR

6WDUW

Fig.4

Fig.5

4. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.5）
5. Shake the tube in Step 4 by overturning it to right and left for 60
times in 1 minute, and then wait for reaction for about 3 minutes.
（Fig.6）Fig.6

6. Gently return the solution in the tube to the Cell when the time countdown reaches under 1 minute, set it again in the cell box. （Fig.7）
7. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.7

CAUTION
Refer to "NO3-N-C1 Nitrate-Nitrogen (High Range) (NO2ー -N ≤0.3 mg/L)".

－ 71 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3

NitrateIn this analyte the procedure should be divided into 3 methods according to the sample state.
Be careful that each method uses their specified reagent.

1. NO 3_1

Nitrate (NO2ー = 0mg/L)

Range：1.0 ー 25.0 mg/L (ppm)
Reagent ：WAK-NO3
Perform the regular Nitrate measurement procedure.

2. NO 3_2

Nitrate (NO2ー ≤ 0.2mg/L)

Range：1.0 ー 25.0 mg/L (ppm)
Reagent ：WAK-NO2, WAK-NO3
It is necessary to zero adjustment with the color-developed sample with reagent for Nitrite (WAK-NO2) before the regular
Nitrate measurement procedure.

3. NO 3_3

Nitrate (NO2ー 0.2 ー 5mg/L)

Range：1.0 ー 25.0 mg/L (ppm)
Reagent ：Pretreatment reagent (NO3-RA), WAK-NO3(C)It is necessary to remove Nitrite by pretreatment reagent before the regular Nitrate measurement procedure.

－ 72 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3_1

Nitrate (NO2ー = 0mg/L)
Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm

Color development： None → Light red → Red
Method： Reduction and Naphthylethylenediamine
Range： 1.0 ー 25.0 mg/L(ppm)
Reagent： WAK-NO3 Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【NO 3 _1】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

=HUR

6WDUW

Fig.2

Fig.3

5. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.3）
6. Shake the tube in Step 5 by overturning it to right and left for 60
times in 1 minute. （Fig.4）

Fig.4

7. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box. （Fig.5）
8. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. As the results tend to vary, note the following points to acquire accurate results.
		 ●	
To suck the sample into the tube, release your finger while depressing the aperture of the tube against the bottom of the Cell tosuck the sample at once.
		 ●	
Immediately after having sucked the sample into the tube, start shaking the tube.
		 ●	
Shake the tube by overturning it to right and left so that the sample moves all the way to both ends of the tube. Shake the tube
for 1 minute (one reciprocation per second).
4. If nitrite ions coexist in the sample, as their color develops stronger than nitrate ions and greatly affects the result, performmeasurement by following the procedures.
			"NO3_2 Nitrate (NO2ー ≤ 0.2 mg/L)"
			"NO3_3 Nitrate (NO2ー 0.2 ー 5 mg/L)"

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level≤ 1000mg/ L ,:	B（Ⅲ）, K+ , Mg2+ , Mn2+ , Na+ , NH 4+ , PO 43 − , Phenol
by adding each of the single substances to the standard solution.
≤ 800mg/L,: Cl −It is not possible to measure seawater.
Oxidizing substance or reductive substance may affect themeasurement.

－ 73 －≤ 200mg/L,: Al3+ , Ca2+ , F − , Ni2+
≤ 100mg/L,: CN − , Fe3+
≤ 50mg/L,: Co2+ , Cr 3+ , Fe2+ , Zn2+
≤ 5mg/L,: Ba2+ , I −≤ 1mg/L,: Cu2+ , Mo（Ⅵ）, Sn2+ , Residual Chlorine≤ 0.5mg/L,: Cr（Ⅵ）, SO32 −< 1mg/L,: NO2 −Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3_2

Nitrate (NO2ー ≤ 0.2mg/L)
Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm

Color development： None → Light red → Red
Method： Reduction and Naphthylethylenediamine
Range： 1.0 ー 25.0 mg/L(ppm)
Reagent： WAK-NO2 Tube, WAK-NO3 Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Pretreatment method
First, develop the color of nitrite ions.
1. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）
2. Suck the whole amount of the sample in the Cell into the tube (WAKNO2) and then lightly shake the tube 5 to 6 times.（Fig.2）

Fig.1

Fig.2

Fig.3

3. Return the solution to the Cell and wait for 3 minutes.（Fig.3）

Procedure
color
developed
sample1. Press【NO3_2】.
2. Press【OK】to switch to the photometry window.

The same Cell

3. Put the Cell containing color-developed nitrite in the cell box andpress【Zero】.（Fig.4）4. Take the Cell out of the cell box, empty it, and clean it with purewater. （Fig.5）
5. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.6）
6. Submerge the aperture of the tube (WAK-NO3) in the sample, and therelease your finger to suck the whole amount of sample at once.
		 At the same time, press【Start】.（Fig.7）

New sample=HUR

color developed
3XUHZDWHU
sample

Fig.4

Fig.5

Fig.6

Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

6WDUW
Fig.7

Fig.8

7. Shake the tube in Step 6 by overturning it to right and left for 60
times in 1 minute. （Fig.8）
8. Return the solution in the tube to the Cell in a gentle manner, set it
again in the cell box. （Fig.9）9. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.9

CAUTION
Refer to "NO3_1 Nitrate (NO2ー = 0 mg/L)".

－ 74 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3_3

Nitrate (NO2ー 0.2 ー 5mg/L)

Color development： None → Light red → Red
Method： Reduction and Naphthylethylenediamine
Range： 1.0 ー 25.0 mg/L(ppm)
Reagent： Pretreatment reagent（NO3-RA）（Pack）, WAK-NO3
Other Items to Use ： Beaker, Heater
Reaction time ： 5 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm
Tube

Pretreatment method
First, remove the nitrite ions mixed in the sample by using pretreatment reagent.
1. Fill a beaker with 30 mL of sample, add pretreatment reagent (NO3-RA)in the sample, and stir the sample 5 to 6 times.（Fig.1）
2. Heat the sample, boil it for 2 minutes, and cool it down to 15 to 30℃ .
（Fig.2）
		 If the amount of the sample has decreased after cooling down, add
pure water up to 30 mL.

NO3-RA

Fig.1

Fig.2

3. Fill the Cell with the pretreated sample for 1.5 mL (up to line). （Fig.3）
Fig.3

Procedure
1. Press【NO 3 _3】.
2. Press【OK】to switch to the photometry window.
3. Put the Cell filled with the pretreated sample in the cell box and press【Zero】.（Fig.4）
4. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.5）

Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

=HUR

6WDUW

Fig.4

Fig.5

Fig.6

Fig.7

5. Shake the tube in Step 4 by overturning it to right and left for 60
times in 1 minute, and then wait for reaction for about 3 minutes.
（Fig.6）
6. Gently return the solution in the tube to the Cell when the time countdown reaches under 1 minute, set it again in the cell box. （Fig.7）
7. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
Refer to "NO3_1 Nitrate (NO2ー = 0 mg/L)".

－ 75 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO 3-N Nitrate-Nitrogen
In this analyte the procedure should be divided into 3 methods according to the sample state.
Be careful that each method uses their specified reagent.

1. NO3-N_1 Nitrate-Nitrogen (NO2ー -N = 0mg/L)
Range：0.20 ー 5.80 mg/L (ppm)
Reagent ：WAK-NO3
Perform the regular Nitrate-Nitrogen measurement procedure.

2. NO3-N_2

Nitrate-Nitrogen (NO2ー -N ≤ 0.06mg/L)

Range：0.20 ー 5.80 mg/L (ppm)
Reagent ：WAK-NO2, WAK-NO3
It is necessary to zero adjustment with the color-developed sample with reagent for Nitrite (WAK-NO2) before the regular
Nitrate-Nitrogen measurement procedure.

3. NO3-N_3

Nitrate-Nitrogen (NO2ー -N 0.06 ー 1.5mg/L)

Range：0.20 ー 5.80 mg/L (ppm)
Reagent ：Pretreatment reagent (NO3-RA), WAK-NO3
It is necessary to remove Nitrite by pretreatment reagent before the regular Nitrate-Nitroegen measurement procedure.

－ 76 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO3-N_1 Nitrate-Nitrogen (NO2ー -N = 0mg/L)
Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm

Color development： None → Light red → Red
Method： Reduction and Naphthylethylenediamine
Range： 0.20 ー 5.80 mg/L(ppm)
Reagent： WAK-NO3 Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【NO 3 -N_1】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

=HUR

6WDUW

Fig.2

Fig.3

5. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.3）
6. Shake the tube in Step 5 by overturning it to right and left for 60
times in 1 minute. （Fig.4）

Fig.4

7. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box. （Fig.5）
8. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

CAUTION
1. The optimum pH during color development is 3. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
2. Perform measurement with the sample temperature set to 15 to 30℃ .
3. As the results tend to vary, note the following points to acquire accurate results.
		 ●	
To suck the sample into the tube, release your finger while depressing the aperture of the tube against the bottom of the Cell tosuck the sample at once.
		 ●	
Immediately after having sucked the sample into the tube, start shaking the tube.
		 ●	
Shake the tube by overturning it to right and left so that the sample moves all the way to both ends of the tube. Shake the tube
for 1 minute (one reciprocation per second).
4. If nitrite ions coexist in the sample, as their color develops stronger than nitrate ions and greatly affects the result, performmeasurement by following the procedures.
			"NO3-N_2 Nitrate-Nitrogen (NO2ー -N ≤ 0.06 mg/L)"
			"NO3-N_3 Nitrate-Nitrogen (NO2ー -N 0.06 ー 1.5 mg/L)"

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
It is not possible to measure seawater.
Oxidizing substance or reductive substance may affect themeasurement.

－ 77 －≤ 1000mg/ L ,:	B（Ⅲ）, K+ , Mg2+ , Mn2+ , Na+ , NH 4+ , PO 43 − , Phenol≤ 800mg/L,: Cl −≤ 200mg/L,: Al3+ , Ca2+ , F − , Ni2+
≤ 100mg/L,:	CN − , Fe3+
≤ 50mg/L,: Co2+ , Cr 3+ , Fe2+ , Zn2+
≤ 5mg/L,: Ba2+ , I −≤ 1mg/L,: Cu2+ , Mo（Ⅵ）, Sn2+ , Residual Chlorine≤ 0.5mg/L,: Cr（Ⅵ）, SO32 −< 1mg/L,: NO2 −Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO3-N_2

Nitrate-Nitrogen (NO2ー -N ≤ 0.06mg/L)
Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm

Color development： None → Light red → Red
Method： Reduction and Naphthylethylenediamine
Range： 0.20 ー 5.80 mg/L(ppm)
Reagent： WAK-NO2 Tube, WAK-NO3 Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Pretreatment method
First, develop the color of nitrite ions.
1. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）
2. Suck the whole amount of the sample in the Cell into the tube (WAKNO2) and then lightly shake the tube 5 to 6 times.（Fig.2）

Fig.1

Fig.2

Fig.3

3. Return the solution to the Cell and wait for 3 minutes.（Fig.3）

Procedure
1. Press【NO 3 -N_2】.

color
developed
sample2. Press【OK】to switch to the photometry window.

The same Cell

3. Put the Cell containing color-developed nitrite in the cell box andpress【Zero】.（Fig.4）4. Take the Cell out of the cell box, empty it, and clean it with purewater. （Fig.5）

New sample=HUR

color developed
3XUHZDWHU
sample

Fig.4

Fig.5

Fig.6

5. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.6）
6. Submerge the aperture of the tube (WAK-NO3) in the sample, and therelease your finger to suck the whole amount of sample at once.
		 At the same time, press【Start】.（Fig.7）
7. Shake the tube in Step 6 by overturning it to right and left for 60
times in 1 minute. （Fig.8）

Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

6WDUW
Fig.7

Fig.8

8. Return the solution in the tube to the Cell in a gentle manner, set it
again in the cell box. （Fig.9）9. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.9

CAUTION
Refer to "NO3-N_1 Nitrate-Nitrogen (NO2ー -N = 0 mg/L)".

－ 78 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

NO3-N_3

Nitrate-Nitrogen (NO2ー -N 0.06 ー 1.5mg/L)

Color development： None → Light red → Red
Method： Reduction and Naphthylethylenediamine
Range： 0.20 ー 5.80 mg/L(ppm)
Reagent： Pretreatment reagent（NO3-RA）（Pack）, WAK-NO3
Other Items to Use ： Beaker, Heater
Reaction time ： 5 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm
Tube

Pretreatment method
First, remove the nitrite ions mixed in the sample by using pretreatment reagent.
1. Fill a beaker with 30 mL of sample, add pretreatment reagent (NO3-RA)in the sample, and stir the sample 5 to 6 times.（Fig.1）
2. Heat the sample, boil it for 2 minutes, and cool it down to 15 to 30℃ .
（Fig.2）
		 If the amount of the sample has decreased after cooling down, add
pure water up to 30 mL.

NO3-RA

Fig.1

Fig.2

3. Fill the Cell with the pretreated sample for 1.5 mL (up to line). （Fig.3）
Fig.3

Procedure
1. Press【NO 3 -N_3】.
Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

2. Press【OK】to switch to the photometry window.
3. Put the Cell filled with the pretreated sample in the cell box and press【Zero】.（Fig.4）

=HUR

6WDUW

Fig.4

Fig.5

Fig.6

Fig.7

4. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.5）
5. Shake the tube in Step 4 by overturning it to right and left for 60
times in 1 minute, and then wait for reaction for about 3 minutes.
（Fig.6）
6. Gently return the solution in the tube to the Cell when the time countdown reaches under 1 minute, set it again in the cell box. （Fig.7）
7. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
Refer to "NO3-N_1 Nitrate-Nitrogen (NO2ー -N = 0 mg/L)".

－ 79 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

OIL-M

Mineral Oil in Water
Cell ： PACKTEST Square Cup
Wavelength ： 660 nm

Color development： Transparent → White Turbidity
Method： Oil Concentration-Turbidimetry with PNIPAAm
Range： 5.0 ー 60.0 mg/L(ppm) (resolution : 0.5 mg/L)
Reagent： WA-OIL-R
Reaction time ： 0 minute
Other Items to Use ： Water Analysis Reagent Set: Oil (Model: WA-OIL)
Preparation Procedure： Read the instruction for the reagent (WA-OIL-R).

Procedure
1. Press【OIL-M】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with pure water (or tap water) for 1.5 mL (up to line).（Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

3XUHZDWHU

=HUR
Fig.1

Fig.2

5. Take out the Cell, discard the pure water, and fill the same Cell with
1.5mL of the solution prepared using "Water Analysis Reagent Set:
Oil".（Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）

3XUHZDWHU

7. The concentration will be automatically displayed.

7KHVDPH&HOO

Fig.3

6ROXWLRQ

6WDUW
Fig.4

CAUTION
In this method, the concentration of fuel oil A, Engine oil in water is measured.
For notes on the operation, refer to the instruction for the reagent (WA-OIL-R).

－ 80 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

OIL-V

Vegetable Oil in Water
Cell ： PACKTEST Square Cup
Wavelength ： 660 nm

Color development： Transparent → White Turbidity
Method： Oil Concentration-Turbidimetry with PNIPAAm
Range： 5.0 ー 60.0 mg/L(ppm) (resolution : 0.5 mg/L)
Reagent： WA-OIL-R
Reaction time ： 0 minute
Other Items to Use ： Water Analysis Reagent Set: Oil (Model: WA-OIL)
Preparation Procedure： Read the instruction for the reagent (WA-OIL-R).

Procedure
1. Press【OIL-V】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with pure water (or tap water) for 1.5 mL (up to line).（Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

3XUHZDWHU

=HUR
Fig.1

Fig.2

5. Take out the Cell, discard the pure water, and fill the same Cell with
1.5mL of the solution prepared using "Water Analysis Reagent Set:
Oil".（Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）

3XUHZDWHU

7. The concentration will be automatically displayed.

7KHVDPH&HOO

Fig.3

6ROXWLRQ

6WDUW
Fig.4

CAUTION
In this method, the concentration of vegetable oil in water is measured.
For notes on the operation, refer to the instruction for the reagent (WA-OIL-R).

－ 81 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

OIL-S

Oil in Soil

Cell ： PACKTEST Square Cup
Color development： Transparent → White Turbidity
Wavelength ： 660 nm
Method： Turbidimetry with PNIPAAm Extraction after Ethanol Elution
Range： Fuel oil A 400 ー 5000 mg/kg (resolution : 100 mg/kg)
Reagent： SOA-OIL-RR R-1（Dissolved in Ethanol）, R-2（Liquid）, R-3（Liquid）
Reaction time ： 0 minute
Other Items to Use ： Colum(CLM-OIL15,15pc), Bottle(SOA-OIL-BT,15pc), Connector(SOA-OIL-C,1pc)
Preparation Procedure： Read the instruction for the reagent (SOA-OIL-RR).

Procedure
1. Press【OIL-S】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with pure water (or tap water) for 1.5 mL (up to line).（Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

3XUHZDWHU

=HUR
Fig.1

Fig.2

5. Take out the Cell, discard the pure water, and fill the same Cell with
1.5 mL of the solution prepared using "Soil Screening Refill Reagent
Set: Oil".（Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）
7. The concentration will be automatically displayed.

3XUHZDWHU
7KHVDPH&HOO

Fig.3(PXOVLRQVDPSOH

6WDUW
Fig.4

CAUTION
1. In this method, the turbidity of the sample (solution) obtained by the reagent (SOA-OIL-RR) is measured and converted into a fueloil A value. For notes on the operation, refer to the instruction for the reagent (SOA-OIL-RR).
2. The displayed value is a fuel oil A value and the calibration curve differs depending on the oil type. If contamination by other oil
than fuel oil A is anticipated, it is possible to make correction by multiplying the displayed value by any of the coefficients below.
				 Engine oil. . . . . .
displayed value × 0.9
				 Fuel oil A . . . . . .
displayed value × 1.0				 Light oil . . . . . .
displayed value × 1.0				 Kerosene . . . . . .
displayed value × 1.5
				 Gasoline . . . . . .
unable to detect
3. The displayed value corresponds to the oil content (mg) per 1 kg of shape-retained soil containing water content.
		 To convert to a value per dry weight, separately measure the moisture content and perform correction accordingly.

－ 82 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Pb-SPK

Lead (SPK)

Cell ： PACKTEST Square Cup
Color development： Yellow → Orange → Red
Wavelength ： 519 nm
Method： Separation and Preconcentration of lead						 by MetaSEP AnaLig® and 4-(2-Pyridylazo) resorcinol
Range： 0.03 ー 0.50 mg/L(ppm)
Reagent： SPK-Pb K-1（Liquid）, K-2（Liquid）, K-3（Liquid）, K-4（Liquid）, Tube
Reaction time ： 3 minutes after drawing sample into the tube.
Preparation Procedure： Read the instruction for the reagent (SPK-Pb).

Procedure
1. Press【Pb-SPK】.
2. Press【OK】to switch to the photometry window.
3. By following the procedures in 5“Collection of eluent" in the usage ofthe reagent (SPK-Pb), take the eluent in the Cell. （Fig.1）

Eluent

=HUR
Fig.1

Fig.2

4. Put the Cell in the cell box and press【Zero】.（Fig.2）
5. Suck the whole amount of the eluent in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Shake the tube in Step 5 about 30 times. If orange lumps are left in
the tube, further shake the tube. （Fig.4）

6WDUW
Fig.3

Fig.4

7. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box. （Fig.5）
8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of the eluent in the reagent (SPK-Pb) is measured. For notes on the operation, refer to the
instruction for the reagent (SPK-Pb).
2. If the concentration of lead ions in the sample is considered high or the result is above the measurement range, dilute the sample
so that the concentration falls within the measurement range, and perform steps again up to collection of eluent using the reagent(SPK-Pb).
3. Perform measurement with the sample temperature set to 15 to 30℃ .
4. Dissolve orange lumps in the tube as much as possible. Undissolved colorless reagent will not affect the measurement.

Influence of coexisting substance
Refer to the instruction for the reagent (SPK-Pb).

－ 83 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Phenol（Reagent Model： WAK-PNL）
Cell ： PACKTEST Square Cup
Wavelength ： 508 nm

Color development： Light yellow → Orange → Red
Method： 4-Aminoantipyrine with enzymeRange： 0.20 ー 5.00 mg/L(ppm)
Reagent： WAK-PNL K-1（Dropper）, Tube
Reaction time ： 8 minutes after drawing sample into the tube.

Procedure
1. Press【Phenol】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add one droplet of K-1 reagent, attach the cap, and shake the Cell 2
to 3 times. （Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.4）

.

6WDUW
Fig.3

Fig.4

7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）
8. After 8 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. This method is suitable only for WAK-PNL. When using WAK-PNL-2, refer to the procedures for“Phenol-2”.
2. Phenols are classified into phenol and p-cresol according to the JIS method, but in this method, the concentration of phenol only ismeasured. Note that the concentration of p-cresol is not measured.
3. The optimum pH during color development is 8. If the pH of the sample is not within the range from 5 to 10, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Oxidizing substance or reductive substance may affect themeasurement.

－ 84 －≤ 1000mg/ L ,:	B（Ⅲ）, Ba2+ , Cl − , F − , I − , K+ , Mg2+ , Na+ , NO2 − , NO3 − ,SO 42 − , Zn2+
≤ 500mg/L,:	Ca2+ , Cd2+ , NH 4+≤ 200mg/L,: As（Ⅲ）, PO 43 − , Anionic Surfactant≤ 100mg/L,:	Mo（Ⅵ）, SCN −≤ 50mg/L,: Ag+ , Cr（Ⅵ）≤ 20mg/L,: Co2+ , Cr 3+ , Cu2+ , Fe3+ , Ni2+ , Residual Chlorine≤ 10mg/L,: Mn2+ , SO32 −≤ 5mg/L,: CN − , Pb2+
≤ 1mg/L,: Al3+ , Fe2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Phenol-2（Reagent Model： WAK-PNL-2）
Cell ： PACKTEST Square Cup
Wavelength ： 525 nm, 670 nm

Color development： Light yellow → Pink → Red
Method： 4-Aminoantipyrine
Range： 0.20 ー 5.00 mg/L(ppm)
Reagent： WAK-PNL-2 Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【Phenol-2】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. This method is suitable only for WAK-PNL-2. When using WAK-PNL, refer to the procedures for“Phenol”.
2. Phenols are classified into phenol and p-cresol according to the JIS method, but in this method, the concentration of phenol only ismeasured. Note that the concentration of p-cresol is not measured.
3. The optimum pH during color development is about 9. If the pH of the sample is not within the range from 4 to 10, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	B（Ⅲ）, Ba2+ , Br－ , Ca2+ , Cl − , F − , I − , K+ , Mg2+ , Mo（Ⅵ）,
Na+ , NH 4+ , NO2 − , NO3 − , PO 43 − , SO 42 − , Zn2+ ,Non-ionic Surfactant , Cationic Surfactant , EDTA ,Citric Acid , Glucose , Sodium Thiosulfate Pentahydrate≤ 500mg/L,:	Al3+ , Ascorbic Acid , Anionic Surfactant≤ 200mg/L,: Ni2+ , Hydrogen Peroxide≤ 100mg/L,: Co2+
≤ 50mg/L,: Fe2+ , Fe3+
≤ 20mg/L,: Cr 3+ , Cu2+ , Residual Chlorine≤ 2mg/L,: Mn2+

Seawater cannot be measured as is.
Please dilute the sample 2-fold or more with pure water, so it will
be measurable.
Oxidizing substance or reductive substance may affect the measurement.
Less than 10% of ethanol does not affect the measurement.

－ 85 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

PO 4-C

Phosphate (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 550 nm

Color development： None → Light blue → Blue
Method： Molybdenum BlueRange
： 2.0 ー 50.0 mg/L(ppm)
Reagent： WAK-PO4（C） K-1（Liquid）, Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【PO4-C】.

.
P/
6DPSOHP/

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 0.2 mL by using the small pipette.
（Fig.1）
4. Add the K-1 reagent for 1.5 mL to the sample in the Cell by using thelarge pipette, attach the cap, and shake the Cell 2 to 3 times.（Fig.2）

Fig.1

Fig.2

5. Remove the cap of the Cell, put the Cell in the cell box and press【Zero】.（Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.4）
7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）

=HUR
Fig.3

6WDUW
Fig.4

8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of ionized phosphate ion(PO43− ) is measured.
		 It is not possible to measure the concentration of hydrolyzable phosphorus and total phosphorus. To measure the concentration ofhydrolyzable phosphorus and total phosphorus, conduct pretreatment according to JIS K 0102 46.
2. Use the small pipette for sample after thoroughly cleaning it with pure water or cleaning its inside with the sample.
3. The optimum pH during color development is 1. If the pH of the sample is not within the range from 1 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample and K-1 reagent temperature set to 20℃ .
		 If their temperature is other than 20℃ , multiplying the measurement value by either of the following coefficients can implement correction.
				 15℃・・・・・×1.05
25℃・・・・・×0.95
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , Cl － , Cr 3+ , Fe2+ , K+ , Mg2+ , Mn2+ ,Na+ , NH 4+ , Ni2+ , NO3 － , SO 42 － , Zn2+ , Phenol≤ 500mg/L,:	NO2 －≤ 200mg/L,:	F － , Fe3+ , Residual Chlorine≤ 100mg/L,:	CN － , Cr（Ⅵ）, Mo（Ⅵ）, Silica≤ 10mg/L,: Cu2+
< 1mg/L,: As（Ⅴ）, Ba2+

Seawater does not affect the measurement.

－ 86 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

PO 4-P-C

Phosphate-Phosphorus (High Range)
Cell ： PACKTEST Square Cup
Wavelength ： 550 nm

Color development： None → Light blue → Blue
Method： Molybdenum BlueRange
： 0.7 ー 15.0 mg/L(ppm)
Reagent： WAK-PO4（C） K-1（Liquid）, Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【PO 4 -P-C】.

.
P/
6DPSOHP/

2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 0.2 mL by using the small pipette.
（Fig.1）
4. Add the K-1 reagent for 1.5 mL to the sample in the Cell by using thelarge pipette, attach the cap, and shake the Cell 2 to 3 times.（Fig.2）

Fig.1

Fig.2

5. Remove the cap of the Cell, put the Cell in the cell box and press【Zero】.（Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.4）
7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）

=HUR
Fig.3

6WDUW
Fig.4

8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of ionized phosphate ion(PO43− ) is measured.
		 It is not possible to measure the concentration of hydrolyzable phosphorus and total phosphorus. To measure the concentration ofhydrolyzable phosphorus and total phosphorus, conduct pretreatment according to JIS K 0102 46.
2. Use the small pipette for sample after thoroughly cleaning it with pure water or cleaning its inside with the sample.
3. The optimum pH during color development is 1. If the pH of the sample is not within the range from 1 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample and K-1 reagent temperature set to 20℃ .
		 If their temperature is other than 20℃ , multiplying the measurement value by either of the following coefficients can implement correction.
				 15℃・・・・・×1.05
25℃・・・・・×0.95
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , Cl － , Cr 3+ , Fe2+ , K+ , Mg2+ , Mn2+ ,Na+ , NH 4+ , Ni2+ , NO3 － , SO 42 － , Zn2+ , Phenol≤ 500mg/L,:	NO2 －≤ 200mg/L,:	F － , Fe3+ , Residual Chlorine≤ 100mg/L,:	CN － , Cr（Ⅵ）, Mo（Ⅵ）, Silica≤ 10mg/L,: Cu2+
< 1mg/L,: As（Ⅴ）, Ba2+

Seawater does not affect the measurement.

－ 87 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

PO 4

Phosphate
Cell ： PACKTEST Square Cup
Wavelength ： 650 nm, 580 nm

Color development： None → Light blue → Blue
Method： Molybdenum BlueRange
： 0.10 ー 5.00 mg/L(ppm)
Reagent： WAK-PO4 K-1（Dropper）, Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【PO 4 】.
2. Press【OK】to switch to the photometry window
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add four droplets of K-1 reagent, attach the cap, and shake the Cell
2 to 3 times. （Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.4）

.

6WDUW
Fig.3

Fig.4

7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）
8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of ionized phosphoric acid (PO43− ) in the sample is measured.
		 It is not possible to measure the concentration of hydrolyzable phosphorus and total phosphorus. To measure the concentrationof hydrolyzable phosphorus and total phosphorus, conduct pretreatment according to JIS K 0102 46 or refer to "TP Total
Phosphorus".
2. The optimum pH during color development is 1. If the pH of the sample is not within the range from 1 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 15℃・・・・・×1.05
25℃・・・・・×0.95

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	B（Ⅲ）, Cl − , CN − , K+ , Na+ , NH4+ , NO3 − , SO42 − , Zn2+
≤ 800mg/L,: Al3+ , Mn2+
≤ 500mg/L,: Mg2+ , NO2 −≤ 100mg/L,: Cr 3+ , Fe2+ , Fe3+ , Ni2+ , Silica , Phenol≤ 50mg/L,: Co2+ , Cu2+ , F − , I − , Residual Chlorine≤ 20mg/L,: Ca2+ , Cr（Ⅵ）, Mo（Ⅵ）< 1mg/L,: As（Ⅴ）, Ba2+

Seawater cannot be measured as is.
Please dilute the sample 5-fold or more with pure water, so it will be measurable.

－ 88 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

PO 4-P

Phosphate-Phosphorus
Cell ： PACKTEST Square Cup
Wavelength ： 650 nm, 580 nm

Color development： None → Light blue → Blue
Method： Molybdenum BlueRange
： 0.03 ー 1.50 mg/L(ppm)
Reagent： WAK-PO4 K-1（Dropper）, Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【PO 4 -P】.
2. Press【OK】to switch to the photometry window
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Add four droplets of K-1 reagent, attach the cap, and shake the Cell
2 to 3 times. （Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.4）

.

6WDUW
Fig.3

Fig.4

7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）
8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of ionized phosphoric acid (PO43− ) in the sample is measured.
		 It is not possible to measure the concentration of hydrolyzable phosphorus and total phosphorus. To measure the concentrationof hydrolyzable phosphorus and total phosphorus, conduct pretreatment according to JIS K 0102 46 or refer to "TP Total
Phosphorus".
2. The optimum pH during color development is 1. If the pH of the sample is not within the range from 1 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 20℃ .
		 If the sample temperature is other than 20 ℃ , multiplying the measurement value by either of the following coefficients canimplement correction.
				 15℃・・・・・×1.05
25℃・・・・・×0.95

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	B（Ⅲ）, Cl − , CN − , K+ , Na+ , NH4+ , NO3 − , SO42 − , Zn2+
≤ 800mg/L,: Al3+ , Mn2+
≤ 500mg/L,: Mg2+ , NO2 −≤ 100mg/L,: Cr 3+ , Fe2+ , Fe3+ , Ni2+ , Silica , Phenol≤ 50mg/L,: Co2+ , Cu2+ , F − , I − , Residual Chlorine≤ 20mg/L,: Ca2+ , Cr（Ⅵ）, Mo（Ⅵ）< 1mg/L,: As（Ⅴ）, Ba2+

Seawater cannot be measured as is.
Please dilute the sample 5-fold or more with pure water, so it will be measurable.

－ 89 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

PO 4-D

Phosphate (Low Range)
Cell ： PACKTEST Square Cup
Wavelength ： 540 nm, 570 nm

Color development： None → Light purple → Purple
Method： 4-Aminoantipyrine with enzyme
Range： 0.10 ー 3.00 mg/L（ppm）
Reagent： WAK-PO4（D） Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【PO 4 -D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized phosphate ion(PO43− ) is measured.
		 It is not possible to measure the concentration of hydrolyzable phosphorus and total phosphorus. To measure the concentration ofhydrolyzable phosphorus and total phosphorus, conduct pretreatment according to JIS K 0102 46.
2. The optimum pH during color development is 7. If the pH of the sample is not within the range from 6 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
It is not possible to measure seawater.
Oxidizing substance such as hydrogen peroxide and residual
chlorine may cause a positive measurement error.
Reductive substance may cause a negative measurement error.

－ 90 －≤ 1000mg/ L ,:	Ba2+ , Ca2+ , Cl− , F − , I − , K+ , Na+ , NH4+ , NO2 − , NO3 −≤ 500mg/L,:	B（Ⅲ）≤ 250mg/L,: Phenol≤ 100mg/L,:	Zn2+≤ 50mg/L,: Cu2+ , Ni2+ , SO 42 −≤ 20mg/L,: Mg2+
≤ 10mg/L,: Al3+ , Cr 3+ , Cr（Ⅵ）≤ 5mg/L,: Fe3+ , Mn2+
≤ 1mg/L,: CN −< 1mg/L,: Fe2+ , Residual ChlorineⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

PO 4-P-D

Phosphate-Phosphorus (Low Range)
Cell ： PACKTEST Square Cup
Wavelength ： 540 nm, 570 nm

Color development： None → Light purple → Purple
Method： 4-Aminoantipyrine with enzyme
Range： 0.03 ー 1.00 mg/L(ppm)
Reagent： WAK-PO4（D） Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Procedure
1. Press【PO 4 -P-D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line).（Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.3）
6. Lightly shake the tube in Step 5 from 5 to 6 times, return thesolution in the tube to the Cell in a gentle manner, set it again in
the cell box.（Fig.4）

6WDUW
Fig.3

Fig.4

7. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized phosphate ion(PO43− ) is measured.
		 It is not possible to measure the concentration of hydrolyzable phosphorus and total phosphorus. To measure the concentration ofhydrolyzable phosphorus and total phosphorus, conduct pretreatment according to JIS K 0102 46.
2. The optimum pH during color development is 7. If the pH of the sample is not within the range from 6 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.
It is not possible to measure seawater.
Oxidizing substance such as hydrogen peroxide and residual
chlorine may cause a positive measurement error.
Reductive substance may cause a negative measurement error.

－ 91 －≤ 1000mg/ L ,:	Ba2+ , Ca2+ , Cl− , F − , I − , K+ , Na+ , NH4+ , NO2 − , NO3 −≤ 500mg/L,:	B（Ⅲ）≤ 250mg/L,: Phenol≤ 100mg/L,:	Zn2+≤ 50mg/L,: Cu2+ , Ni2+ , SO 42 −≤ 20mg/L,: Mg2+
≤ 10mg/L,: Al3+ , Cr 3+ , Cr（Ⅵ）≤ 5mg/L,: Fe3+ , Mn2+
≤ 1mg/L,: CN −< 1mg/L,: Fe2+ , Residual ChlorineⒸ 2023 Kyoritsu Chemical-Check Lab.,Corp

S

Sulfide (Hydrogen Sulfide)
Cell ： PACKTEST Square Cup
Wavelength ： 670 nm, 620 nm

Color development： None → Light blue → Blue
Method： Methylene blue Method
Range： 0.05 ー 0.80 mg/L(ppm)
Reagent： WAK-S K-1（Dropper）, Tube
Reaction time ： 3 minutes after drawing sample into the tube.

Procedure
1. Press【S】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

5. Add two droplets of K-1 reagent. （Fig.3）

Fig.2'RQRWVKDNH

6. Immediately suck the whole amount of the sample in the Cell into thetube and press【Start】at the same time. （Fig.4）

.

6WDUW

7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）

Fig.3

Fig.4

8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, the concentration of sulfur in the hydrogen sulfide (H 2 S), ionized hydrogen sulfide (HS － ) and sulfide ion (S 2－ )states is measured. It is not possible to measure the concentration of sulfuric acid and sulfurous acid.
2. In the case where the sulfide existing in the sample is considered sulfide ions (S 2－ ) only, the obtained result can be converted into
the concentration of hydrogen sulfide by multiplying it by 1.06.
3. The optimum pH during color development is 2. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level Except for Heavy metal ions:by adding each of the single substances to the standard solution.
≤ 100mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , K+ , Mg2+ , Na+ , NH 4+ , NO3 − ,Seawater does not affect the measurement.
Oxidizing substance or reductive substance may affect themeasurement.
If metallic ions are mixed with sulfide ions, metallic sulfide is
produced and it cannot be detected as sulfide ions any longer.
In this case, perform separation of metallic sulfide by referring to
the JIS method or others.

PO 43 − , SO 42- , Phenol , Anionic Surfactant≤ 10mg/L,: I −≤ 1mg/L,: NO2 − , SO32< 1mg/L,: Residual Chlorine

Heavy metal ions:－ 92 －≤ 10mg/ L ,:	Al3+ , Ba2+ , CN − , Co2+ , Cr 3+ , Fe2+ , Fe3+ , Ni2+ , Zn2+
≤ 5mg/L,: Mn2+ , Mo（Ⅵ）≤ 1mg/L,: Cr（Ⅵ）< 1mg/L,: Cu2+

Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

SiO 2

SilicaColor development： None → Light blue → Blue
Method： Molybdenum BlueRange
： 3.0 ー 60.0 mg/L(ppm)
Reagent： WAK-SiO2 Distilled water, K-1（Dropper）, K-2（Dropper）, Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 650 nm, 560 nm

Procedure
1. Press【SiO 2 】.
2. Press【OK】to switch to the photometry window.

6DPSOHP/:DWHUIRU'LOXWLRQP/

3. Fill the Cell with the sample for 0.2 mL by using the small pipette.
（Fig.1）
4. Add the Water for Dilution for 1.5 mL to the sample in the Cell by using
the large pipette, attach the cap, and shake the Cell 2 to 3 times.
（Fig.2）

=HUR
Fig.1

Fig.2

5. Remove the cap of the Cell, put the Cell in the cell box and press【Zero】.（Fig.3）.

6. Add two droplets of K-1 reagent, attach the cap, shake the Cell 2 to
3 times, and leave for 3 minutes.（Fig.4）

Fig.4

7. Add one droplet of K-2 reagent, attach the cap, shake the Cell 2 to 3
times, and leave for 30 seconds.（Fig.5）.

8. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.6）
9. Lightly shake the tube in Step 8 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.7）
10. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.3

Fig.5

6WDUW
Fig.6

Fig.7

CAUTION
1. While silica is classified into ionized silica, dissolved and colloidal silica and total silica and each of them is displayed as silicon
dioxide (SiO2), in this method, the concentration of ionized silica (SiO32－ ) is measured. To measure the dissolved and colloidal silica andtotal silica, perform measurement after pretreatment of the respective measurement targets according to JIS K 0101 44.2 or 44.3.
2. Use the small pipette for sample after thoroughly cleaning it with pure water or cleaning its inside with the sample.
3. The optimum pH during color development is 2. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
4. Perform measurement with the temperatures of the sample and dilution water set to 15 to 30℃ .
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Oxidizing substance or reductive substance may affect themeasurement.
Even a tiny amount of hydrogen sulfide may obstruct themeasurement. If hydrogen sulfide is considered to coexist,
acidify and boil the sample to remove hydrogen sulfide before
performing measurement.

－ 93 －≤ 5000mg/L,:	Al3+ , B（Ⅲ）, Ca2+ , Cl − , CN − , Co2+ , Fe2+ , I − , K+ , Mg2+ ,Mn2+ , Mo（Ⅵ）, Na+ , NH 4+ , Ni2+ , NO3 − , SO 42 − , Zn2+ ,Anionic Surfactant , Residual Chlorine , Phenol ,Formaldehyde≤ 1000mg/L,:	Cr（Ⅵ）, Cu2+ , F − , Fe3+ , NO2 −≤ 500mg/L,:	P O 43 −≤ 100mg/L,:	Ba2+ , Cr 3+≤ 50mg/L,: V（Ⅴ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

SiO 2-D

Silica (Low Range)

Color development： None → Light blue → Blue
Method： Molybdenum BlueRange
： 0.30 ー 7.00 mg/L(ppm)
Reagent： WAK-SiO2（D） K-1（Dropper）, K-2（Dropper）, Tube
Reaction time ： 5 minutes after drawing sample into the tube.

Cell ： PACKTEST Square Cup
Wavelength ： 650 nm, 560 nm

Procedure
1. Press【SiO 2 -D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）
Fig.1

Fig.2

5. Add two droplets of K-1 reagent, attach the cap, shake the Cell 2 to
3 times, and leave for 3 minutes.（Fig.3）
6. Add one droplet of K-2 reagent, attach the cap, shake the Cell 2 to 3
times, and leave for 30 seconds.（Fig.4）

.

Fig.3

7. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.5）
8. Lightly shake the tube in Step 7 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.6）.

Fig.4
9. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

6WDUW
Fig.5

Fig.6

CAUTION
1. While silica is classified into ionized silica, dissolved and colloidal silica and total silica and each of them is displayed as silicon
dioxide (SiO2), in this method, the concentration of ionized silica (SiO32－ ) is measured. To measure the dissolved and colloidal silica andtotal silica, perform measurement after pretreatment of the respective measurement targets according to JIS K 0101 44.2 or 44.3.
2. The optimum pH during color development is 2. If the pH of the sample is not within the range from 2 to 9, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the temperatures of the sample and dilution water set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
Seawater does not affect the measurement.
Oxidizing substance or reductive substance may affect themeasurement.
Even a tiny amount of hydrogen sulfide may obstruct themeasurement. If hydrogen sulfide is considered to coexist,
acidify and boil the sample to remove hydrogen sulfide before
performing measurement.

－ 94 －≤ 1000mg/ L ,:	Al3+ , B（Ⅲ）, Ca2+ , Cl − , CN − , Co2+ , Fe2+ , I − , K+ , Mg2+ ,Mn2+ , Mo（Ⅵ）, Na+ , NH 4+ , Ni2+ , NO3 − , SO 42 − , Zn2+ ,Anionic Surfactant , Residual Chlorine , Phenol ,Formaldehyde≤ 500mg/L,:	NO2 −≤ 200mg/L,:	Cr（Ⅵ）≤ 100mg/L,:	Cu2+ , F − , Fe3+
≤ 50mg/L,: PO 43 −≤ 10mg/L,: Ba2+ , Cr 3+ , V（Ⅴ）Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

SO 4

SulfateCell ： PACKTEST Square Cup
Wavelength ： 615 nm

Color development： Transparent → White Turbidity
Method： Barium sulfate turbidimetryRange： 5 ー 100 mg/L(ppm)
Reagent： DPRーSO4 RーA（Dropper）, R-1（Dropper）, R-2（Dropper）
Reaction time ： 3 minutes after R-2 reagent is added.

Procedure
1. Press【SO4】.
2. Press【OK】to switch to the photometry window.

R-A

3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）
Fig.1

Fig.2

4. Add one droplet of the R-A reagent, attach the cap, and shake the
Cell 2 to 3 times. （Fig.2）
5. Add one droplet of the R-1 reagent after shaking it intensely, attach
the cap, and shake the Cell 2 to 3 times. （Fig.3）
5

6. Remove the cap of the Cell, put the Cell in the cell box and press【Zero】.（Fig.4）

=HUR
Fig.3

Fig.4

7. Add one droplet of the R-2 reagent, attach the cap, shake the Cell 30
times or more in 10 seconds, and press【Start】.（Fig.5）
8. Remove the cap of the Cell, set the Cell in the cell box again. （Fig.6）5

9. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

6WDUW
Fig.5

Fig.6

CAUTION
1. In this method, the concentration of ionized sulfuric acid (SO42－ ) is measured.
2. The optimum pH during color development is about 2. If the pH of the sample is not within the range from 2 to 9, neutralize thesample with dilute hydrochloric acid or dilute sodium hydroxide solution, etc. (Do not use sulfuric acid.)
3. Perform measurement with the sample temperature set to 20 to 30℃ .
4. Depending on the operation method, the results vary. In Step 7 of "Procedure", shake the Cell in a constant manner. If the Cell isshaken gently, the measurement value tends to be low. If the Cell is shaken intensely, the measurement value tends to be high.
5. To set the Cell in the cell box, remove the cap. Wipe off water droplets before setting the Cell in the cell box.
6. As turbid substances attach to the Cell after measurement, thoroughly clean the Cell.

Influence of coexisting substance
The stored calibration curve has been created by using the standard solution. If the influence of other substance is considered,check the measurement value by comparing it with the official method or by standard addition method.
The right chart is the list of interference data for acceptable level by adding each of the single substances to the standard solution.
It is possible to measure seawater, but as it has a high concentrationof sulfate ions, dilution is necessary. (Approximately 100 times
in the case of artificial seawater)
Substances such as sulfite ions and thiosulfuric acid ions turninto sulfate ions and are measured depending on their oxidation
state.
If anions that cause production of insoluble barium salt arecontained under the acid condition, measurement is notpossible.

Except for Heavy metal ions:≤ 1000mg/ L ,:	B（Ⅲ）, Ca2+ , Cl − , F − , K+ , Na+ , NH 4+ , NO2 − , NO3 − ,Phenol≤ 500mg/L,:	PO 43 −≤ 200mg/L,:	Residual Chlorine< 1mg/L,: Anionic Surfactant
Heavy metal ions:－ 95 －≤ 200mg/L,:	Fe3+≤ 100mg/L,:	Cr（Ⅵ）≤ 20mg/L,:	Al3+Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

TH

Total Hardness
Cell ： PACKTEST Square Cup
Wavelength ： 569 nm, 550 nm, 670 nm

Color development： Light purple → Purple
Method： Phthalein Complexone
Range： 10 ー 150 mg/L(ppm)
Reagent： WAK-TH Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【TH】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.3）
6. Shake the tube in Step 5 about 10 times. (If the reagent is left in thetube, further shake the tube.)（Fig.4）

6WDUW
Fig.3

Fig.4

7. Gently return the solution in the tube to the Cell, set it again in thecell box. （Fig.5）
8. After 1 minutes have elapsed, the concentration will be automaticallydisplayed.
Fig.5

CAUTION
1. In this method, a sample having the ratio between calcium hardness and magnesium hardness of 2:1 to 3:1 can be measured. The
calibration curve has been created using 2.5:1 as the standard solution.
2. A sample with a higher percentage of calcium hardness produces a higher result, and a sample with a higher percentage ofmagnesium hardness produces a lower result.
3. If the total hardness value in the sample is 10 mg/L or less, color development will occur, but the result will be displayed as"UNDER".
4. The optimum pH during color development is 10. If the pH of the sample is not within the range from 5 to 10, neutralize thesample with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
5. Perform measurement with the sample temperature set to 15 to 30℃ .

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	Cl － , K+ , Na+ , NH 4+ , NO2 － , NO3 － , PO 43 － , SO 42 － ,Anionic Surfactant , Phenol≤ 100mg/L,:	B（Ⅲ）, F － , I － , Silica≤ 50mg/L,: Ba2+
≤ 10mg/L,: CN － , Mo（Ⅵ）≤ 5mg/L,: Al3+
≤ 1mg/L,: Co2+ , Cr（Ⅵ）, Ni2+ , Residual Chlorine< 1mg/L,: Cr 3+ , Cu2+ , Fe2+ , Fe3+ , Mn2+ , Zn2+

It is not possible to measure seawater.

－ 96 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

TN-2

Total Nitrogen

Color development： Light red → Red
Method： Decomposition by Potassium Peroxodisulfate under Alkaline Condition						 + Reduction and Naphthylethylenediamine
Range： 0.5 ー 7.0 mg/L（ppm）
Reagent： TNP-N-R
Reaction time ： 5 minutes after drawing sample into the tube.
Other Items to Use ： Mini Autoclave Set (Model: TNP-MAS), Heater
Preparation Procedure： Read the instruction for the reagent (TNP-N-R).

Cell ： PACKTEST Square Cup
Wavelength ： 539 nm, 580 nm

Procedure
1. Press【TN-2】.
2. Press【OK】to switch to the photometry window.
3. Take the whole amount of the pretreated sample cooled to 20℃ in the
Cell, and add two droplets of R-3 reagent, attach the cap, and shake
the Cell 2 to 3 times. （Fig.1）
4. Remove the cap of the Cell, put the Cell in the cell box and press【Zero】.（Fig.2）
5. Submerge the aperture of the tube in the sample, and the release
your finger to suck the whole amount of the sample at once. At thesame time, press【Start】.（Fig.3）

5
Pretreatedsample

=HUR

Fig.1

Fig.2

Immerse the hole of the tube
to the bottom of the Cell, andthen release the tube in a flash.
The tube will not fall.

6WDUW
Fig.3

Fig.4

6. Shake the tube in Step 5 by overturning it to right and left for 60
times in 1 minute.（Fig.4）
7. Immediately return the solution in the tube to the Cell in a gentle
manner, set it again in the cell box.（Fig.5）

Fig.5

8. After 5 minutes have elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of nitrogen in the form of ionized nitrate ion (NO3 ー ) in the pretreated sample is measured.
2. After the pretreated sample has been cooled down to 20℃ , conduct the measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	B（Ⅲ）, K+ , Mg2+ , Na+ , PO43 − , Phenol≤ 200mg/L,:	Al3+ , Ca2+ , F − , Ni2+
≤ 100mg/L,:	Fe3+≤ 50mg/L,: Co2+ , Zn2+
≤ 5mg/L,: Ba2+
≤ 1mg/L,: Cu2+ , Mo（Ⅵ）≤ 0.5mg/L,: Cr（Ⅵ）

Measure seawater after diluting it by 10 times and pretreatment it.
Oxidizing substance or reductive substance may affect themeasurement. (However, in this measurement method, reductive
substances are not considered left in the pretreated sample.)－ 97 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

TP-2

Total Phosphorus

Color development： None → Light blue → Blue
Method： Decomposition by Potassium Peroxodisulfate under Acid Condition						 + Molybdenum blueRange
： 0.10 ー 2.00 mg/L（ppm）
Reagent： TNP-P-R
Reaction time ： 3 minutes after drawing sample into the tube.
Other Items to Use ： Mini Autoclave Set (Model: TNP-MAS), Heater
Preparation Procedure： Read the instruction for the reagent (TNP-P-R).

Cell ： PACKTEST Square Cup
Wavelength ： 650 nm, 580 nm

Procedure
1. Press【TP-2】.
2. Press【OK】to switch to the photometry window
3. Take the whole amount of the pretreated sample cooled to 20℃ in the
Cell.（Fig.1）

Pretreated sample

=HUR
Fig.1

Fig.2

4. Put the Cell in the cell box and press【Zero】.（Fig.2）
5. Add four droplets of R-3 reagent, attach the cap, and shake the Cell
2 to 3 times.（Fig.3）
6. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time.（Fig.4）

R-3

6WDUW
Fig.3

Fig.4

7. Lightly shake the tube in Step 6 from 5 to 6 times, return the solution
in the tube to the Cell in a gentle manner, set it again in the cell box.
（Fig.5）
8. After 3 minutes have elapsed, the concentration will be automaticallydisplayed.

Fig.5

CAUTION
1. In this method, the concentration of ionized phosphoric acid (PO43－ ) in the pretreated sample is measured.
2. After the pretreated sample has been cooled down to 20℃ , conduct the measurement.
		 When its temperature is between 30℃ to 40℃ , an approximate result can be obtained by multiplying the measurement value by 0.8.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	B（Ⅲ）, Cl − , CN − , K+ , Na+ , NO3 − , SO 42 − , Zn2+
≤ 800mg/L,: Al3+
≤ 500mg/L,:	Mg2+≤ 100mg/L,:	Fe3+ , Ni2+ , Silica≤ 50mg/L,: Co2+ , Cu2+ , F −≤ 20mg/L,: Ca2+ , Cr（Ⅵ）, Mo（Ⅵ）< 1mg/L,: As（Ⅲ）, Ba2+

Seawater does not affect the measurement.

－ 98 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Turbid-F

Turbidity（Formazine）
Cell ： PACKTEST Square Cup
Wavelength ： 660 nm

Measurement of turbidity of sample
Calibration 		 ： Formazine Turbidity standard solution
Measurement range： 10 to 400 deg.
Reagent 		 ： not used
Measurement time： 0 minute

Procedure
1. Press【Turbid-F】.
2. Press【OK】to switch to the photometry window.
Pure water

3. Fill the Cell with pure water for 1.5 mL (up to line). （Fig.1）=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Fig.1

Fig.2

5. Take out the Cell, discard the pure water, fill the same Cell with 1.5
mL of sample. （Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）

The same Cell
Sample

6WDUW

3XUHZDWHU

7. The Turbidity will be automatically displayed.

Fig.3

Fig.4

CAUTION
Formazine turbidity is obtained in this method based on JIS K 0101 9.2 Transmitted-light turbidity.
The principle of this method is different from Scattered-light turbidity(NTU Nephelometric Turbidity Unit).

－ 99 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Turbid-P

Turbidity（Polystyrene）
Cell ： PACKTEST Square Cup
Wavelength ： 660 nm

Measurement of turbidity of sample
Calibration 		 ： Polystyrene Turbidity standard solution
Measurement range： 10 to 100 deg.
Reagent 		 ： not used
Measurement time： 0 minute

Procedure
1. Press【Turbid-P】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with pure water for 1.5 mL (up to line). （Fig.1）
4. Put the Cell in the cell box and press【Zero】.（Fig.2）

Pure water

=HUR
Fig.1

Fig.2

5. Take out the Cell, discard the pure water, fill the same Cell with 1.5
mL of sample. （Fig.3）
6. Set the Cell in the cell box again and press【Start】.（Fig.4）

The same Cell
Sample3XUHZDWHU

7. The Turbidity will be automatically displayed.

Fig.3

6WDUW
Fig.4

CAUTION
Perform measurement after sufficiently shaking the sample.

－ 100 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp

Zn-D

Zinc (Low Range)
Cell ： PACKTEST Square Cup
Wavelength ： 553 nm

Color development： Yellow → Orange → Pink
Method
： 5-Br-PAPS
Range： 0.02 ー 0.40 mg/L(ppm)
Reagent： WAK-Zn（D） K-1（Small Pack）, K-2（Liquid）, Tube
Reaction time ： 1 minute after drawing sample into the tube.

Procedure
1. Press【Zn-D】.
2. Press【OK】to switch to the photometry window.
3. Fill the Cell with the sample for 1.5 mL (up to line). （Fig.1）

.

=HUR

4. Put the Cell in the cell box and press【Zero】.（Fig.2）
Fig.1

Fig.2

Fig.3

5. Add K-1 reagent, attach the cap, and sufficiently shake the Cell tocompletely dissolve the reagent. （Fig.3）
6. Add K-2 reagent for 0.3 mL using pipette, attach the cap and shake
the Cell 2 to 3 times, and then remove the cap and let the Cell standfor 5 minutes. （Fig.4）

5

.

min
Fig.4

6WDUW
Fig.5

7. Suck the whole amount of the sample in the Cell into the tube andpress【Start】at the same time. （Fig.5）
8. Lightly shake the tube in Step 7 about 30 times. （Fig.6）
9. Return the solution in the tube to the Cell in a gentle manner, set it
again in the cell box. （Fig.7）

Fig.6

Fig.7

10.After 1 minute has elapsed, the concentration will be automaticallydisplayed.

CAUTION
1. In this method, the concentration of ionized zinc (Zn2+) in the sample is measured.
		 If result of zinc concentration including suspension and precipitate is required, dissolve zinc in advance and then performmeasurement.
2. The optimum pH during color development is 9. If the pH of the sample is not within the range from 5 to 10, neutralize the sample
with dilute sulfuric acid or dilute sodium hydroxide solution, etc.
3. Perform measurement with the sample temperature set to 15 to 30℃ .
4. Dissolve orange lumps in the tube as much as possible. Undissolved colorless reagent will not affect the measurement.
5. Use of a measuring pipette or the like instead of the supplied pipette enables more accurate measurement.

Influence of coexisting substance
The stored calibration curve has been created by using thestandard solution. If the influence of other substance isconsidered, check the measurement value by comparing itwith the official method or by standard addition method.
The right chart is the list of interference data for acceptable levelby adding each of the single substances to the standard solution.

≤ 1000mg/ L ,:	B（Ⅲ）, Ba2+ , Ca2+ , Cl － , F － , I － , K+ , Mg2+ , Mo（Ⅵ）,
Na+ , NH 4+ , NO2 － , NO3 － , PO 43 － SO 42 － , Phenol≤ 50mg/L,: Residual Chlorine , Anionic Surfactant≤ 20mg/L,: CN － , Cr 3+≤ 10mg/L,: Ag+ , Al3+ , Cr（Ⅵ）≤ 1mg/L,: Co2+ , Cu2+ , Mn2+ , Fe2+ , Fe3+ , Ni2+

Seawater does not affect the measurement.

－ 101 －Ⓒ 2023 Kyoritsu Chemical-Check Lab.,Corp