GHS mark

Lead

CautionINSTRUCTIONS

1. PACKTEST Lead allows measuring dissolved lead ions. If you wish to measure total leadModelconcentration including suspended particles, refer to the following section "Measuring of lead

Separation and preconcentration of leadby MetaSEP AnaLig R
and PAR Visual Colorimetric Method

DangerRange ： Pb 0〜1 mg/L（ppm）
Main reagent ： PAR 4-（2-Pyridylazo）resorcinol

SPK−PbRequired time ： 10 minutes

including suspended particles ".
2. Sample including many suspended particles or precipitation needs filtration.
Column Quantity：20

Connector Quantity：2

10mL-Cup

Sulfuric Acid is necessary to dilute fewer than 10%.

10mL-Syringe

Quantity：1mL

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910

connecting or strong pressure will cause leaks or splash of liquid. Wear eye protector, mask and gloves.
8. Solid deposits under low temperature have no influence to measure.

Add K-1 reagent

9. At［Procedure ⑯］, shake enough to dissolve the orange reagent.

K-1 Reagent
Quantity：1

2.5mL-Syringe

Quantity：1

7. K-1 reagent, K-2 reagent, K-3 reagent and sample added K-1 reagent are strong acid. Loose

10. To measure under 0.1mg/L with high accuracy, refer to the following section "Measuring of Under

Quantity：1

Quantity：3

Material
Material Load K-1,2,3 reagent
Load sample to the column PPPE
to the column

K-2 Reagent
Quantity：1

K-3 Reagent
Quantity：1

K-4 Reagent
Quantity：1

Material Recovering MaterialPS
waste liquid PP

Tube Quantity：20（4 laminated package）

2.5

1mL-PipettemL

PP（Single-use）

Connecting a columnand syringes
Material Taking 10mL Material Recovering
PPPP（Useable ten times）
of a sample
an eluent

2of Nitric Acid.
6. Keep sample temperature in the range 15℃ - 40℃.

Separation andpreconcentration of lead Material

0.5

5. Sample is required pH≤1 passing through the column. If it is pH >1, add more K-1 reagent or 10%

100mL-PlasticBeaker

512as aqua regia, H2O2 and Cl2. F , pH≥10

Quantity：1

10

4. Following conditions should be avoided passing thorough the column. Strong oxidizing agents such−Cell（PACKTEST
Square Cup）

Quantity：1

1.5

Sulfuric Acid. Sample including strong acid more than 10% as Nitric Acid, Hydrochloric Acid andItems ＆ Usage

1

3. Sample pH≥9 adjust under pH7 with diluted Nitric Acid or diluted Hydrochloric Acid excluding

PbMaterial
MaterialPP
Color developing reagent PE

5mL-Cup

Quantity：1

Standard Color
Quantity：1

0.05mg/L of lead".

標準色

Caution Strong acid

Measuring of lead including suspended particles
10mL of sample in a heat-resisting beaker with K-1 reagent and boil until suspended particles dissolved.

（10% HNO3）

Caution Strong acid（10% HNO3）

Sample pH Material Conditioningadjustment PPof the column

ー5ー4ー3ー2ー1

Caution Strong acid（10% HNO3）

Material WashingMaterial Eluent
the column PPPP

Material Taking
K-4 reagent
PPMaterialPPDetection Materialof lead
Paper

After cooling, pour the sample to the 10mL-cup and add pure water up to 10mL line.
Then, start from［Procedure 2.］.

Measuring of Under 0.05mg/L of lead
Recommend passing through more volume of sample to the column to measure under 0.05mg/L

FeatureMetaSEP AnaLig R is used to concentrate Pb2+ from dilute samples and separate Pb2+ frominterfering substances. Then Pb2+ reacts with PAR to form a red colored complex in direct
proportion to the Pb2+ concentration. It is suitable for wastewater management.

of Pb2+.

Handling of PACKTEST Before and After use

Example:If a sample needs 50mL, add 5mL of R-1 reagent.

K-1 reagent, K-2 reagent, K-3 reagent and sample solution after K-1 reagent addition will be Strongly-acidic.

Then pass through 50mL of the sample to the column.
To obtain the result, divide the measurement value by 5.

First Aid

If the irritation persists or get worse, please seek medical attention immediately.
Especially in case ingested reagents or test solution, drink plently of water or milk
and immediately seek medical attention.
Please refer to GHS label on the package.

Standard colors are determined from Standard Solutions. However, coexisting substances willcause inaccurate results. The list below reports substances concentrations under which ones
interferences are insignificant:Insoluble complexes with CrO42-、Cr2O72-、F-、PO43- and SO42- are not able to measure.

Seek medical attention.

Skin contact → Immediately flush skin with water.
Enter into mouth → Immediately rinse mouth. Seek medical attention.

Interferences≤10000mg/L ・・・ Ascorbic Acid, Citric Acid, Glutamic Acid, Oxalic Acid, Tartaric Acid, Thiourea≤1000mg/L ・・・ B（Ⅲ）, Ca2+, Cl-, Mg2+, Na+, NO3-, Chlorine, EDTA, IDA≤500mg/L ・・・ K+≤100mg/L ・・・ Ag+, Al3+, Cr3+, Cu2+, Fe2+, Fe3+, Mn2+, Mo（Ⅵ）, Ni2+, Sn2+, Zn2+,
Anionic Surfactant, Nonionic Surfactant≤1mg/L ・・・ Ba2+≤0.5mg/L ・・・ Bi3+

Eye contact → Immediately rinse eyes with water for at least 15 minutes.

StrageUse PACKTEST tubes as soon as possible after opening the laminated pakage.

Disposal

Dispose this product in a manner consistent with Federal, State, and local regulations.

5-37-11, Den-Eenchofu, Ota-ku, Tokyo, 145-0071, JAPANFAX : +81-3-3721-0666 E-mail:eng@kyoritsu-lab.co.jp
http://kyoritsu-lab.co.jp/english/

Please read the back side by all means.

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Procedure

Lead is concentrated in a column, then eluted from the column, and detected by colorimetry.

1.The sample pHK-1
adjustment reagent

3.Loadingthe sample sample

105

Lead become trapped
to a column

columneluent

4.WashingK-3
the column reagent

2.Conditioning
K-2of the column reagent

5.The eluent
K-4
recovery reagent
Eluting lead from the column

5.The eluent recovery① Take exactly 10mL of the sample③ Use a 2.5mL-syringe to④ Use a 10mL-syringe to pass through the prepared⑥ Use another 2.5mL-syringe to⑧ Prepare the Cell（PACKTEST Square Cup）.

to 10mL-cup. （Fig.1）

pass through 2 mL of K-2

sample to the conditioned column for 3minutes.

② Use a 1mL-pipette toreagent to the column for

Caution Strong
acidreagent until 1.5mL line accurately.

for details".
⑦ Screw out the 2.5mL-syringe

air

Fig.2

⑪ Pass through the column for 30

into the 2.5mL- syringe, andseconds and receive the eluent tothen eject the liquid left in thethe cell.（Fig.6）

column.（Fig.3）

Fig.3（Fig.5）from the connector. Draw air※ See below "Instruction for details".

air

K-4

1.5mL line
K-4

0.5sample

until 1mL line, and then suck up K-4K-4

1mL

Fig.1

12512345678
91010sample※ See below "Instruction

2.5mL
syringe

1.55"Instruction fordetails".

⑩ Draw air into another 2.5mL-syringemL

10⑤ Screw out the 10mL-syringe
from the connector. Draw
air into the 10mL-syringe,
and then eject the liquid
left in the column.（Fig.3）

5mL-Cup.（Fig.4）

2※ See below

syringe

Caution Strongacid⑨ Take 1.5-2mL of K-4 reagent to2.5for details".

airto the column for 20 seconds.

1K-1

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Caution Strongacid

mL0

Swirl to mix.（Fig.2）※ See below "Instruction

1230 seconds.

← 1mL

pass through 1 mL of K-3 reagent

0.5

4.Washing the column

1.5

3.Loading the sample

2

2.Conditioning of the column

mL

1.The sample pH adjustment

2.5※Wear a tool for protection such as eye protecter, a mask, gloves.
※Please perform all the following procedure at one sample.

add 1mL of K-1 reagent.

6.Detection of lead

ー5ー4ー3ー2
ー1

Fig.4

eluent

Fig.5

Fig.6

6.Detection of lead
⑫Open the laminated package.

⑬Press the sides ofRemove the line to clear

the tube to expel

the cell.

the aperture fromapproximately

half

Release the side andof volume. Maintaindraw the eluent inpressed. （Fig.8）

the tube.（Fig.9）

the top of the＜Pb＞
marktube.（Fig.7）

Pull out
the line.

⑭ Immerse the tube in⑮Shake the tube lightly about
Immerse the hole of the tube
to the bottom of the cell, andthen release the tube in a flash.
The tube will not fall.

⑯After 1 minute, compare with

30 times.（Fig.10）

the Standard Color.（Fig.11）
1min.

Fig.11How to read the test
30 times
Fig.8

Fig.7

Fig.9

Fig.10After the reaction time, compare the color of the tube with the Standard Color.
The nearest color indicates the measured value of the sample.
A color between two standard colors indicates a value between them.
2.5
mL

Instruction for details

The main operation of PACKTEST Lead is loading liquid to a column. Repeat procedure of a - e as each reagent and sample.

2

2.5

mL

1.5

2
● To prevent contamination of reagents,● Screw

2.5
mL

2connector

1.5
10.5

volume of liquid with turning up the syringe.

Danger, K-2, K-3 reagents and sample are strong acid.

columnRecommended flow rate.
K-2 reagent : over 30seconds,
K-3 reagent : over 20seconds
K-4 reagent : over 30seconds,
sample : over 3 minutes● Receive the K-2, K-3 reagents and sample

flowed thorough the column to the 100mLplastic beaker, because they are strong acidity.

0.5● To avoid eye contact, do not measure theK-2、K-3● Do not remove the column

and the connector until
finish all procedure.

avoid splashing liquid as disconnect.

1

doesn't become influence whenreagents or sample pass through thecolumn.

● Loose● Do not push syringe by strong pressure to

1.5● Airsyringe2● K-4 reagent is required accurate volume.

connecting willcause leaks or splashof liquid.

0.5

columnclick

approximate volume.

syringe1connector● K-2 and K-3 reagents are allowed the1.5● Connector is used about 10times. If it would becomeleaks, use new one.

in the syringe
to the connector tight.

syringes are rinsed with pure water
before use.

leaks of liquid.

e. Screw out the syringe fromthe connector.

mL● Loose connecting will cause

d. Slow flow rate are desirable.

2.5

c. Connect syringes with liquid
and the connector（female).

K-2
K-3
sampleSlowlyCaution Strongacid

is single-use but
connector is not. Do notdispose connector withcolumn.

0.5

b. Suck up reagents or sample into syringes.

1a. Fasten the column to the connector（male）until it makes a clicking sound.

syringe● Column

connector
column● Pull the column horizontallyfrom the connector forreleasing.
● Rinse the connector withpure water after and before
use.

Please read the back side by all means.

6031