JRD Vol. 70 (4) の発刊のお知らせ
SRD 会員の皆様へ
平素から The Journal of Reproduction and Development (JRD) の刊行にご協力を頂き有難うございます。Vol.
70 (4) が発刊となりました。ご一読いただき，また論文執筆の際にはご活用いただけますようお願いいたします。
本号では，2023 年に SRD 奨励賞を受賞された久保田 海雄 先生に招待総説論文を寄稿していただきました。
是非お読みいただけましたら幸いです。
JRD 編集委員長 原山 洋-----------------------------------------------------PubMed Central of JRD
https://www.ncbi.nlm.nih.gov/pmc/journals/2339/-----------------------------------------------------JRD Vol. 70 (4)https://www.jstage.jst.go.jp/browse/jrd/70/4/_contents/-char/en
SRD Young Investigator Award 2023
Molecular approaches to mammalian uterine receptivity for conceptus implantation
Kaiyu KUBOTA
Volume 70 Issue 4 Pages 207-212
DOI https://doi.org/10.1262/jrd.2024-022
Cover Story:
Uterine receptivity is the specific state during early pregnancy when the endometrium is ready to accept the embryo
or conceptus, and this process by which the embryo attaches to the endometrium is also known as implantation.
Kubota summarized the molecular mechanisms underlying steroid hormone-induced uterine receptivity and thesimilarities and differences in this mechanism among different mammals (Kubota K. Molecular approaches tomammalian uterine receptivity for conceptus implantation, pp. 207–212). This review will help understand theimportance of uterine receptivity and the challenges associated with alleviating implantation failure. Investigating
the mechanism of uterine receptivity is necessary to develop diagnostic and therapeutic tools for successful
pregnancies.
Original Article
Alpha-synuclein expression in oxytocin neurons of young and old bovine brains
Yvan Bienvenu NIYONZIMA, Yuuki ASATO, Hiroya KADOKAWA
Volume 70 Issue 4 Pages 213-222
DOI https://doi.org/10.1262/jrd.2024-020

Excluding alanine from minimum essential medium (MEM) nonessential amino acid supplementation of the culture
medium facilitates post-fertilization events and early cleavages of bovine oocytes fertilized in vitro
Nobuhiko ITAMI, Satoshi AKAGI, Yuji HIRAO
Volume 70 Issue 4 Pages 223-228
DOI https://doi.org/10.1262/jrd.2023-098
Oocyte activation with phospholipase Cζ mRNA induces repetitive intracellular Ca2+ rises and improves the qualityof pig embryos after intracytoplasmic sperm injection
Michiko NAKAI, Shun-ichi SUZUKI, Dai-ichiro FUCHIMOTO, Shoichiro SEMBON, Kazuhiro KIKUCHI
Volume 70 Issue 4 Pages 229-237
DOI https://doi.org/10.1262/jrd.2023-105
Role and action mechanisms of tPA in CRH-induced apoptosis of mouse oviductal epithelial and mural granulosa
cells
Yong-Qing YANG, Min ZHANG, Qi HUA, Rui-Jie MA, Xiao-Yan WANG, Hong-Jie YUAN, Ming-Jiu LUO, JingHe TAN
Volume 70 Issue 4 Pages 238-246
DOI https://doi.org/10.1262/jrd.2024-028
Resveratrol intake by males increased the mitochondrial DNA copy number and telomere length of blastocysts
derived from aged mice
Noko TERAMOTO, Yuri OKADA, Nao ABURADA, Masamune HAYASHI, Jun ITO, Komei SHIRASUNA,
Hisataka IWATA
Volume 70 Issue 4 Pages 247-253
DOI https://doi.org/10.1262/jrd.2024-043
Technology Report
Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda
epididymal spermatozoa
Misuzu IDE, Ibuki SAITO, Makoto SANBO, Mito KANATSU-SHINOHARA, Takashi SHINOHARA, Masumi
HIRABAYASHI, Shinichi HOCHI
Volume 70 Issue 4 Pages 254-258
DOI https://doi.org/10.1262/jrd.2024-030
Evaluation of bovine embryo quality based on gene expression profiling using whole-transcriptome amplification
Takashi FUJII, Takamasa MUKAI, Shoji HASEGAWA, Toh-ichi HIRATA, Ken SAWAI
Volume 70 Issue 4 Pages 259-263
DOI https://doi.org/10.1262/jrd.2024-007

-----------------------------------------------------JRD Vol. 70 (3)https://www.jstage.jst.go.jp/browse/jrd/70/3/_contents/-char/en
SRD Outstanding Research Award 2023
Role of chemokines in regulating luteal and uterine functions in pregnant cows
Ryosuke SAKUMOTO
Volume 70 Issue 3 Pages 145-151
DOI https://doi.org/10.1262/jrd.2023-100
Cover Story:
Chemokines are known to regulate various reproductive functions, such as regulation of corpus luteum (CL) anduterine functions in cows. However, the role of chemokines in pregnancy has not yet been fully elucidated. Sakumotosummarized and reviewed the literature on chemokine (-receptor) expression and its physiological roles in the bovine
CL and uterus during pregnancy. This review will help understand the mechanisms of chemokine-mediatedinteractions among the CL, uterus, immune cells, and conceptus during pregnancy in cows.
SRD Young Investigator Award 2023
Chromatin structure in totipotent mouse early preimplantation embryos
Masatoshi OOGA
Volume 70 Issue 3 Pages 152-159
DOI https://doi.org/10.1262/jrd.2023-106
Original Article
Effect of ovarian stimulation on developmental speed of preimplantation embryo in a mouse model
Mayuko KURUMIZAKA, Tatsuma YAO, Mikiko TOKORO, Noritaka FUKUNAGA, Yoshimasa ASADA, Kazuo
YAMAGATA
Volume 70 Issue 3 Pages 160-168
DOI https://doi.org/10.1262/jrd.2023-089
Lipopolysaccharide-binding protein in follicular fluid is associated with the follicular inflammatory status andgranulosa cell steroidogenesis in dairy cows
Fumie MAGATA, Misato KIKUZAWA, Heinrich BOLLWEIN, Fuko MATSUDA, Shingo HANEDA
2024 Volume 70 Issue 3 Pages 169-176
DOI https://doi.org/10.1262/jrd.2023-104
Social effects on behaviorally-scored and pedometer-detected estrus in beef cattle
Hiromi KUSAKA, Minoru SAKAGUCHI
Volume 70 Issue 3 Pages 177-183

DOI https://doi.org/10.1262/jrd.2024-005
Physiological high temperatures alter the amino acid metabolism of bovine early antral follicles
Kohei KAWANO, Kenichiro SAKAGUCHI, Nattapong NINPETCH, Yojiro YANAGAWA, Seiji KATAGIR
Volume 70 Issue 3 Pages 184-191
DOI https://doi.org/10.1262/jrd.2023-096
Changes in interleukin-2, -4, -6 and -8 expression in the postovulatory sow endometrium after artificial insemination
based on conceived or failed to conceive
Minami W. OKUYAMA, Masaharu MORIYOSHI, Seiji KATAGIRI
Volume 70 Issue 3 Pages 192-196
DOI https://doi.org/10.1262/jrd.2023-094
DNA repair is efficient in irradiated M phase zygotes
Yuan WANG, Dai TSUKIOKA, Shoji ODA, Hiroshi MITANI, Fugaku AOKI
Volume 70 Issue 3 Pages 197-201
DOI https://doi.org/10.1262/jrd.2024-018
Technology Report
Effect of globin peptide on female fertility in aging granulosa cell-specific Nrg1 knockout mice
Takashi UMEHARA, Marino OGASAHARA, D.M.V. Supun PREMARATHNE, Yuka SASAKAWA, Yasuo
SUMIDA, Masayuki SHIMADA
Volume 70 Issue 3 Pages 202-206
DOI https://doi.org/10.1262/jrd.2023-076-----------------------------------------------------JRD Vol. 70 (2)https://www.jstage.jst.go.jp/browse/jrd/70/2/_contents/-char/en
Obituary (Special thanks)
In memory of Dr. Ryuzo Yanagimachi (Yana) (1928–2023)
Teruhiko WAKAYAMA, Atsuo OGURA
Volume 70 Issue 2 Pages i-iv
DOI https://doi.org/10.1262/jrd.2024-E01
Original Article
Identification and characterization of dystrophin-locus-derived testis-specific protein: A testis-specific gene within
the intronic region of the rat dystrophin gene
Keitaro YAMANOUCHI, Shizuka KATO, Yukie TANAKA, Masanari IKEDA, Yukina OSHIMO, Takanori SHIGA,Kei HATAMOTO, James CHAMBERS, Takuya IMAMURA, Ryuji HIRAMATSU, Kazuyuki UCHIDA, Fuko
MATSUDA, Takashi MATSUWAKI, Tetsuya KOHSAKA
Volume 70 Issue 2 Pages 55-64

DOI https://doi.org/10.1262/jrd.2023-073
Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse
blastocysts
Nao ABURADA, Jun ITO, Yuki INOUE, Taiyo YAMAMOTO, Masamune HAYASHI, Noko TERAMOTO, Yuri
OKADA, Yuichi KOSHIISHI, Koumei SHIRASUNA, Hisataka IWATA
Volume 70 Issue 2 Pages 65-71
DOI https://doi.org/10.1262/jrd.2023-079
Lipid droplets synthesized during luteinization are degraded after pregnancy
Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi
TSUKAMOTO
Volume 70 Issue 2 Pages 72-81
DOI https://doi.org/10.1262/jrd.2023-095
Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle
Atsushi SHIRASAWA, Masafumi HAYASHI, Mayumi SHONO, Atsushi IDETA, Takashi YOSHINO, Katsuhiko
HAYASHI
Volume 70 Issue 2 Pages 82-95
DOI https://doi.org/10.1262/jrd.2023-087
Cover Story:
The process of inducing the germ cell lineage from pluripotent stem cells, referred to as in vitro gametogenesis, aids
in comprehending the mechanisms involved in germ cell differentiation and offers an alternative source of gametes
for reproduction. Shirasawa et al. have developed a novel method for robust induction of primordial germ cell-likecells (PGCLCs) from newly established bovine embryonic stem (bES) cells. After a 24-hour culture with bonemorphogenetic protein 4 (BMP4), followed by a three-dimensional culture with BMP4 and chemicals modulating
WNT signaling, bES cells exhibited positive expression for a set of primordial germ cell (PGC) markers, including
PRDM1/BLIMP1, TFAP2C, and SOX17. These outcomes are anticipated to have practical implications for thedevelopment of stem cell-based reproductive technologies in cattle.
Progesterone and estradiol regulate sperm hyperactivation and in vitro fertilization success in mice
Miyu FUJIKURA, Masakatsu FUJINOKI
Volume 70 Issue 2 Pages 96-103
DOI https://doi.org/10.1262/jrd.2023-080
Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking
testis-epididymis lumicrine signaling
Daiji KIYOZUMIVolume 70 Issue 2 Pages 104-114
DOI https://doi.org/10.1262/jrd.2023-102
Parallel expression patterns of NR4A nuclear receptor family genes in the pituitary gland of proestrus rats
Ryota TERASHIMA, Daiki NAGAO, Masato IKEO, Keisuke MORIOKA, Titaree LAOHARATCHATATHANIN,
Shiro KURUSU, Mitsumori KAWAMINAMI
Volume 70 Issue 2 Pages 115-122
DOI https://doi.org/10.1262/jrd.2023-090

Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes
Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO
Volume 70 Issue 2 Pages 123-130
DOI https://doi.org/10.1262/jrd.2023-067
High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm
Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru
TAKEO
Volume 70 Issue 2 Pages 131-137
DOI https://doi.org/10.1262/jrd.2023-085
The fertility of dairy heifers and cows is not influenced by the follicular wave of the ovulatory follicle
Javad MOHAMMADI, Mehdi AZARI, Mojtaba KAFI
Volume 70 Issue 2 Pages 138-143
DOI https://doi.org/10.1262/jrd.2023-084-----------------------------------------------------JRD Vol. 70 (1)https://www.jstage.jst.go.jp/browse/jrd/70/1/_contents/-char/en
Review
Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control
Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu
ISHIKAWA-YAMAUCHI, Takuya SATO
Volume 70 Issue 1 Pages 1-9
DOI https://doi.org/10.1262/jrd.2023-093
Cover Story:
For the past century, achieving in vitro spermatogenesis has remained a difficult challenge for researchers. In 2011,
Ogawa et al. successfully demonstrated in vitro spermatogenesis in mice using an organ culture method. However,extending this method to other species posed challenges for over a decade. In 2023, Ogawa’s team achieved in vitro
spermatogenesis in rats by incorporating several critical modifications to enhance their original technique. This
review presents a detailed analysis by Ogawa et al. comparing their method with natural in vivo conditions and othersynthetic alternatives. They systematically explore the merits, limitations, and inherent constraints of the organ
culture approach, delving into the specifics of medium composition, the principles of the gas-liquid interphasemethod, use of microfluidic devices, and innovation of the PDMS-ceiling method. Highlighting the challenges faced,
including regulating oxygen concentration, managing tissue formation, and regulating culture space-control. The
insights and novel concepts shared in this review are particularly valuable for those involved in culture or related
disciplines, providing innovative content, and encouraging further exploration in this field.
Original Article
PABPN1L is required for maternal mRNA degradation after meiosis resumption
Chihiro EMORI, Mayo KODANI, Ferheen ABBASI, Masashi MORI, Masahito IKAWA
Volume 70 Issue 1 Pages 10-17
DOI https://doi.org/10.1262/jrd.2023-077
Lipid droplet formation is spatiotemporally regulated in oocytes during follicular development in mice
Ryutaro AIZAWA, Megumi IBAYASHI, Junichiro MITSUI, Satoshi TSUKAMOTO
Volume 70 Issue 1 Pages 18-24

DOI https://doi.org/10.1262/jrd.2023-055
Development of a fluorometric Cuboni test for the semi-quantitative measurement of urinary estrogen levels andpregnancy detection in mares
Kaede ODA, Maya YOSHIDA, Abdul Razaq IRSHAD, Tomomi KANAZAWA, Toru TAKAHASHI
Volume 70 Issue 1 Pages 25-29
DOI https://doi.org/10.1262/jrd.2023-083
Equine chorionic gonadotropin treatment and timed artificial insemination for dairy cow production under heat stress
Daisuke FUNAKOSHI, Hidetoshi SHIOTANI, Makoto SEKI
Volume 70 Issue 1 Pages 30-34
DOI https://doi.org/10.1262/jrd.2023-069
Negative photoperiod induces an increase in the number of ovulations in dairy cattle
Fernando LÓPEZ-GATIUS
Volume 70 Issue 1 Pages 35-41
DOI https://doi.org/10.1262/jrd.2023-075
Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early
development of bovine in vitro fertilized embryos
Norikazu MIYASHITA, Satoshi AKAGI, Tamas SOMFAI, Yuji HIRAO
Volume 70 Issue 1 Pages 42-48
DOI https://doi.org/10.1262/jrd.2023-031
Technology Report
Capturing temperature changes on the ocular surface along with estrus and ovulation using infrared thermography inJapanese Black cows
Riho OZAKI, Seiji INOUE, Yuki YOROZUI, Rei ICHIKAWA, Naoki YAMADA, Seiya HIGASHI, Shuichi
MATSUYAMA, Hiroko TSUKAMURA, Satoshi OHKURA, Yoshihisa UENOYAMA, Yasuhiro MORITA
Volume 70 Issue 1 Pages 49-54
DOI https://doi.org/10.1262/jrd.2022-116