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Glasses. Shop by category.Tissue-typed humaninterferon-alpha is a potent inhibitor of humanmelanoma growth in vitro and in vivo. A direct
correlation between cellular proliferation and IFNalpha-mediated growth suppression is establishedfor a number of tumors. To test whether a similar
relationship exists for human melanoma, theauthors examined the effects of IFN-alpha on thegrowth of a variety of human melanoma cell lines

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in vitro and in a xenograft model in vivo.
Fourteen cell lines were assayed for IFN-alphamediated growth inhibition. Cells were exposed
to recombinant human IFN-alpha for a period oftime ranging from 24 to 96 hr. Proliferation of
the tumors was measured by 3H-thymidine
incorporation, and tumor volumes and IFN-alphabinding was measured by immunofluorescence.
To examine IFN's antitumor effects in vivo,
human melanoma cells were implanted into nude
mice and then injected with increasing doses ofIFN-alpha. Melanoma cell growth was assessedby tumor weight and 3H-thymidine incorporation.
Tumor volume was assessed by caliper
measurements. Seventy-five percent of melanoma
cell lines were growth inhibited by IFN-alphatreatment. The extent of IFN-alpha-mediatedgrowth inhibition correlated well with tumor cellproliferation in vitro. In vivo IFN-alpha had adose-dependent effect on human melanoma
growth. Antitumor activity was increased withpage 5 / 6prolonged exposure to IFN-alpha. Humanmelanoma appears to be an ideal tumor for the
study of IFN-alpha-mediated growth inhibition,
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Quite Imposing Serial Key Rev 011012