第回国際歯科研究学会日本部会総会・学術大会

69

The 69th Annual Meeting ofJapanese Association for Dental Research

第 69 回 国際歯科研究学会

日本部会総会・学術大会
Re-defining the Mission of Dental
Research towards Post-corona WorldPHOTO : Fumio Hashimoto

October 24 (Sun.) – October 25 (Mon.), 2021
Hybrid Meeting（Onsite at Kyushu University and Online）「写真提供：福岡市」

PROGRAM
AND
ABSTRACTS OF PAPERS

JAPANESE ASSOCIATION FOR
DENTAL RESEARCH

The 69th ANNUAL MEETING
October 24-25, 2021
Hybrid Meeting (Onsite at Kyushu University and Online)

CONTENTS

Officers of JADR …………………………………………………………………

4

JADR Timetable ……………………………………………………………………

5

Scientific Program …………………………………………………………………

7(Abstracts)
Special Lecture ……………………………………………………………………

22

Greeting ……………………………………………………………………………

24

KADR President Lecture …………………………………………………………

26

The 1st Joint Symposium of KADR and JADR ……………………………………

30

Symposium (I ~Ⅱ) …………………………………………………………………

38

Rising Scientist Session ……………………………………………………………

55

Poster Presentation …………………………………………………………………

65

Author Index ………………………………………………………………………

97

List of Sponsors …………………………………………………………………… 101

─2─

Welcome Message
69th Japanese Association for Dental Research General Session &Exhibition
I would like to welcome all of you to attend 69th JADR General
Session & Exhibition, which will be held on October 24th and 25th.
Due to the pandemic of Covid-19, it will be held both at onsite(Kyushu University Hospital Campus) and online (hybrid holding).
I assume all the attendants have been struggling and asking themselves as to what we could havedone and what we can do in future for our society in this difficult situation caused by Covid-19
pandemic. In this meeting, I would like you to discuss about this issue each other. Therefore, themain theme of this meeting was decided as “Re-defining the Mission of Dental Research
towards Post-corona World”. To begin to discuss about this issue, we will welcome Dr. Hiroshi
Kiyono, former president of Japanese Society for Immunology, as special lecturer. I am so surethat his lecture not only suggest us thoughtful consideration as a top leading immunologist but
also provide us encouraging message as a dentist. Besides this special lecture, we will hold 1st
Japan-Korea co-operative symposium which was originally planned last year, and other special
symposiums entitled “Japan-Originated Next Generation Biomedical Materials”, “Current
Progress of Stem Cell Biology and Regenerative Medicine in Dental Science”. I am confident
that the symposiums provide us most up-dated topics on regenerative dentistry and dental
biomaterials. We also hold “Rising Scientist Symposium” which was just started last year.
We really wished to host the meeting on site here in Fukuoka. However, unfortunately, thispandemic is still threatening us all over the country, and therefore, we chose hybrid holding.
Although we may not discuss each other face to face, we would like to provide opportunity to
re-define our important mission imposed to the researchers in dental field toward post-corona
world.
Sincerely,
FUSANORI NISHIMURA
President, 69th JADR General Session & Exhibition
Section of Periodontology, Division of Oral Rehabilitation,
Kyushu University Faculty of Dental Science

─3─

OFFICERS
OFJAPANESE ASSOCIATIONFOR DENTAL RESEARCH

President ………………………………………………………………………… S. Nakamura
President-elect and Vice-president ……………………………………………… K. Moriyama
Immediate Past President ……………………………………………………… S. Imazato
Treasurer ………………………………………………………………………… M. Hayashi
Director ………………………………………………………………………… H. Egusa
Director ………………………………………………………………………… T. Goto
Director ………………………………………………………………………… K. Ikebe
Director ………………………………………………………………………… T. Matsumoto
Director ………………………………………………………………………… F. Nishimura
Director ………………………………………………………………………… M. Noda
Director ………………………………………………………………………… T. Nomura
Director ………………………………………………………………………… S. Yamada
Director ………………………………………………………………………… A. Yoshihara
Auditor ………………………………………………………………………… T. Takata
Auditor ………………………………………………………………………… K. Yamazaki

Chairman, Organizing Committee ……………………………………………… F. Nishimura
Secretary-general ……………………………………………………………… T. Sanui

─4─

JADR Timetable October 24th(Sun.), 25th(Mon.)
Video Distribution/ Live Streaming
October 24th (Sun.), 2021

9:00

October 25th (Mon.), 2021

Opening Ceremony

8:55 〜 9:00
9:00 〜 9:15

Greeting from IADR President
9:15 〜 9:25 Greeting from KADR President
9:25 〜 10:15

KADR President Lecture
10:00

Symposium I
Japan-Originated Next Generation
Biomedical Materials
Dr. Koichiro Hayashi (Kyushu University)
Dr. Takashi Sawase (Nagasaki University)
Dr. Osamu Suzuki (Tohoku University)
Dr. Shinya Murakami (Osaka University)

Dr. Kung-Rock Kwon (Kyung Hee University)

10:15 〜 10:30

9:00 〜 10:40

Break

10:30 〜 11:30
10:40 〜 11:00

BreakSpecial Lecture
11:00

11:00 〜 12:30

Dr. Hiroshi Kiyono (The University of Tokyo)

Rising Scientist Sesssion
11:30 〜 12:30

12:00

Dr. Shigeki Suzuki (Tohoku University Hospital)
Dr. Ryutaro Kuraji (The Nippon Dental University)
Dr. Kazuhiro Omori (Okayama University Hospital)
Dr. Misaki Iwashita (Kyushu University)

Council Meeting / General Assembly

12:30 〜 13:30

12:30 〜 14:00

Break

13:00

Break
13:30 〜 15:30

14:00

14:00 〜 15:40

JADR/KADR Joint Symposium

Symposium II
Current progress of Stem Cell Biology andRegenerative Medicine in Dental Science

Dr. Keigo Yoshizaki (Kyushu University)
Dr. Jeong-Ho Yun (Jeonbuk National University)
Dr. Masahiro Saito (Tohoku University)
Prof. Kung-Rock Kwon (Kyung Hee University)

Dr. Satoshi Fukumoto (Kyushu University)
Dr. Koichiro Iohara (National Center for Geriatrics andGerontology)
Dr. Issei Saitoh (Asahi University)
Dr. Takao Fukuda (Kyushu University Hospital)

15:00

15:40 〜 15:45

16:00

─5─

Closing Ceremony

JADR Timetable
October 24th(Sun.) - 25th.(Mon.)
Video and Poster File distribution

Greeting from IADR President (Video distribution)
Prof. Eric Reynolds(President of IADR)

Greeting from KADR President
KADR President Lecture (Video distribution)
Prof. Kung-Rock Kwon
(President of KADR)“Esthetic & Functional oral rehabilitation : 4 control factors”

Special Lecture (Video distribution)
Prof. Hiroshi Kiyono(Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The
University of Tokyo)“Past, Present and Future of Mucosal Vaccine: Vital Contributions Made by Dentistry”

Poster Presentation (Poster File distribution of presentation file (PDF))

─6─

October 24th, Sunday, ~ October 25th, Monday
Greeting from IADR President(Video distribution)
October 24th, Sunday, 9:00-9:15
G-1

Dr. Eric Reynolds
President of IADR

Greeting from KADR President
KADR President Lecture(Video distribution)
October 24th, Sunday, 9:15-10:15

KL

Dr. Kazunori Ikebe(Department of Prosthodontics, Gerodontology and Oral Rehabilitation,
Osaka University Graduate School of Dentistry)“Esthetic & Functional oral rehabilitation : 4 control factors”
Dr. Kung-Rock Kwon,
President of KADR
(Department of Prosthodontics School of Dentistry, Kyung Hee University, Seoul, South Korea)

Special Lecture(Video distribution)
October 24th, Sunday, 10:30-11:30
Moderator: Dr. Seiji Nakamura(Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences,
Faculty of Dental Science, Kyushu University)
SL“Past, Present and Future of Mucosal Vaccine: Vital Contributions Made by Dentistry”
Dr. Hiroshi Kiyono(Division of Mucosal Immunology, IMSUT Distinguished Professor Unit
The Institute of Medical Science, The University of Tokyo)

The 1st Joint Symposium of KADR and JADR
(Video distribution and Live Session)
October 24th, Sunday, 13:30-15:30
The 1st Joint Symposium of KADR and JADR
“Regenerative medicine/Dentistry “Organizer/ Moderator: Dr. Hiroshi Egusa(Division of Molecular and Regenerative Prosthodontics, Tohoku
University Graduate School of Dentistry)

─7─

JS-1

Comprehensive analysis for the genes associated with epithelial-mesenchymal interactions in toothDr. Keigo Yoshizaki(Department of Orthodonitcs and Dentofacial Orthopedics, Faculty of Dental Science, Kyushu University)
JS-2 Periodontal regeneration by BMP7-overexpressing bone marrow mesenchymal stem cells in a ligature-induced periodontitis rat model
Dr. Jeong-Ho Yun(Department of Periodontology, Jeonbuk National University College of Dentistry)JS-3 Exacerbation mechanism of alveolar bone destruction in apical periodontitis caused by inflammatory
bowel disease.
Dr. Masahiro Saito(Department of Ecological Dentistry, Division of Operative Dentistry,
Tohoku University Graduate School of Dentistry)JS-4 Implant Supported Overdentures for the Elderly Edentulous Patients
Professor, Kung-Rock Kwon
(Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, Korea)

Symposium Ⅰ(Video distribution and Live Session)
October 25th, Monday 9:00-10:40
Symposium I“Japan-Originated Next Generation Biomedical Materials “Organizer/ Moderator: Dr. Yasunori Ayukawa(Division of Oral Rehabilitation, Department of Dental Science, Kyushu University Faculty of Dental Science)
SI-1 Honeycomb scaffolds: vertical bone augmentation and antibacterial effects
Dr. Koichiro Hayashi(Department of Biomaterials, Faculty of Dental Science, Kyushu University)
SI-2 Bone quality management effects of newly developed dental implant design
Dr. Takashi Sawase(Department of Applied Prosthodontics, Institute of Biomedical Sciences, Nagasaki University)
SI-3 Bioactive performance of octacalcium phosphate and its composite materials
Dr. Osamu Suzuki(Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry)SI-4 Periodontal regeneration by cytokine therapy- its present status and future perspectiveDr. Shinya Murakami(Department of Periodontology Osaka University Graduate School of Dentistry)Rising Scientist Session(Video distribution and Live Session)
October 25th, Monday 11:00-12:30
Rising Scientist Session

─8─“Periodontal Medicine “Organizer/ Moderator: Dr. Kazuhisa Yamazaki(Niigata University Gradeuate School of Medical and Dental Sciences)RS-1 PPARγ-induced global H3K27 acetylation is required to maintain the abilities of extracellular matrixorganization and osteo/cementogenesis in periodontal ligament fibroblasts: the possible link between
dietary unsaturated fatty acids and periodontal tissue homeostasis
Dr. Shigeki Suzuki(Department of Periodontology, Tohoku University Hospital)
RS-2 Microbiome-targeted Probiotic Therapy Prevents Hepatic Lipid-metabolism Abnormality following
Polymicrobial-Periodontal Infection via Improving Oral and Gut Dysbiosis
Dr. Ryutaro Kuraji(Department of Life Science Dentistry, The Nippon Dental University, Tokyo, Japan)
RS-3 Infertility and Periodontitis
Dr. Kazuhiro Omori(Department of Periodontics and Endodontics, Division of Dentistry,Okayama University Hospital, Japan)
RS-4 The role of chronic low-grade inflammation on energy expenditure: association with CCL19/CCR7
axis
Dr. Misaki Iwashita(Section of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science,
Kyushu University)

Symposium Ⅱ(Video distribution and Live session)
October 25th, Monday 14:00-15:40
SymposiumⅡ“Current progress of Stem Cell Biology and Regenerative Medicine in Dental Science “Organizer/ Moderator: Dr. Takayoshi Yamaza(Oral Biological Sciences, Department of Dental Science, Kyushu University Faculty of Dental Science)
SⅡ-1 Single-cell RNA sequence from mouse incisor and molar reveals dental epithelial cell-type specificgenes
Dr. Satoshi Fukumoto(Section of Pediatric Dentistry, Kyushu University Faculty of Dental Science)
SⅡ-2 Recent Progress of Pulp Regenerative Therapy with Dental Pulp Stem Cells for the Application to
the Periapical Disease in the Aged.
Dr. Koichiro Iohara(National Center for Geriatrics and Gerontology, Research Institute,
Geroscience Research Center, Regenerative Dental Medicine)
SⅡ-3 Induced tissue-specific stem cell, a non-tumorigenic intermediate cell induced from somatic or pluripotent cell: their generation and possible use in the regenerative medicine of dental science
Dr. Issei Saitoh(Department of Pediatric Dentistry, Asahi University School of Dentistry)
SⅡ-4 Exosomes secreted from TNF-α-preconditioned gingival tissue-derived stem cells enhance M2 mac-─9─rophage polarization and inhibit periodontal bone loss
Dr. Takao Fukuda(Section of Periodontology, Division of Oral Rehabilitation, Kyushu University Hospital)

─ 10 ─

Poster Presentation
Cariology Research-Microbiological Studies/Biofilm
001: Collagen-binding properties of Streptococcus mutans killed by amoxicillin

Y. SUEHIRO, S. MATAYOSHI, M. OTSUGU, R. NOMURA, K. NAKANO
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan

002: S-PRG eluate inhibits acid and glucan synthesis by Streptococcus mutans

T. KITAMURA, S. MATAYOSHI, J. OHATA, Y. SUEHIRO, R. NOMURA, K. NAKANO
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan

003: Microbiological effects of silver diamine fluoride : an in situ study

K. KLANLIANG1, Y. ASAHI1, H. MAEZONO1, T. SHIMAOKA1, N. KURIKI1, H. MACHI2,
S. EBISU1, M. HAYASHI1
1
Department of Restorative Dentistry and Endodontology, Graduate School of Dentistry, Faculty of
Dentistry, Osaka University, 2Dental Technology Institute, Osaka University, Osaka, Japan

004: Investigation of Streptococcus mutans collagen-binding protein pathogenicity

D. MATSUOKA, S. NAKA, K. GOTO, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry andPharmaceutical Sciences, Okayama, Japan

005: Analysis of specific Streptococcus mutans strains associated with non-alcoholicsteatohepatitis

K. TABATA, S. NAKA, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry andPharmaceutical Sciences, Okayama, Japan

006: Inhibitory effect of cyclodextran on caries in animal model

H. ASAUMI, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry andPharmaceutical Sciences, Okayama, Japan

007: Role of ABC transporter in biofilm formation by Streptococcus mutans

K. GOTO, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry andPharmaceutical Sciences, Okayama, Japan

Craniofacial Biology Research008: BCOR mediated regulation of ZFPM2 via BCL6 involved in hyperactive root
formation of OFCD syndrome

K.M. SOE, T. OGAWA, K. MORIYAMA
Department of Maxillofacial Orthognathics, Tokyo Medical and Dental University (TMDU), Tokyo,Japan─ 11 ─

Dental Materials 3: Metal-based Materials and Other Materials
009: In vitro degradation test of resorbable GBR membrane, Cytrans ElaShield
Y. SAKAGUCHI, K. YAMANAKA, F. FUSEJIMAGC Corporation, Tokyo, Japan

Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
010: The effects of MTA exposed to NaOCl on odontoblastic differentiation of a humanperiodontal ligament stem cell line

K. YAMASHITA1, A. TOMOKIYO2, T. ONO2, K. IPPOSHI1, A. ALHASAN1, A. TSUCHIYA3,
A. HAMANO2,4, H. SUGII1, S. YOSHIDA2, T. ITOYAMA2, H. MAEDA1,2
1
Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan, 2Department of Endodontology, Kyushu University Hospital, Fukuoka,
Japan, 3Department of Biomaterials, Faculty of Dental Science, Kyushu University, Fukuoka, Japan,
4
OBT Research Center Faculty of Dental Science, Kyushu University, Fukuoka, Japan

011: Bone reconstruction using three-dimensional interconnected porous CO3Ap block
fabricated based on hydrate expansion of CaO granules.

K. TANAKA1,2, A. TSUCHIYA2, Y. OGINO1, Y. AYUKAWA1, K. ISHIKAWA2
Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan, 2Department of Biomaterials, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan1

Dental Materials 2: Polymer-based Materials
012: Development of Novel Filler-Dispersed Resin Composite for 3D-Printed Permanent
Crown

P. KARNTIANG1,2, H. IKEDA1, Y. NAGAMATSU1, H. SHIMIZU1
1
Division of Biomaterials, Department of Oral Functions, Kyushu Dental University, Fukuoka, Japan,
2
Division of Operative Dentistry, College of Dental Medicine, Rangsit University, Pathum-Thani,
Thailand

Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
013: Morphological observation of microvascular changes and bone formation after
Alveolar bone regeneration.
M. MATSUO, M. TO
Department of Clinical Oral Anatomy, Kanagawa Dental University, Yokosuka, Japan

014: Tuning macrophage polarization by titanium nanosurfaces through nanotopographic
cues to prevent peri-implantitis
N. KARTIKASARI, M. YAMADA, H. EGUSADivision of Molecular and Regenerative Prosthodontics, Tohoku University graduate school of
Dentistry, Sendai, Japan

Dental Materials 6: Instruments and Equipment
015: Effect of repeated use of end mill on the internal fitness of complete crown.

K. NOZAKI, K. KOMINE, W. YANAKA, M. MATSUMURA, S. OMNIA, C. SHIN,
M. MATSUMURA, H. MIURA
Department of Fixed Prosthodontics, Graduate School of Medical and Dental Sciences, Tokyo─ 12 ─

Medical and Dental University, Tokyo, Japan

Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
016: A novel bioabsorbable polysaccharide derivative for minimally invasive bone graft
harvesting

K. NAKANISHI1, T. HASEGAWA2, H. HAYASHI3, K. NAGAMOTO4, H. OGUMA1,
T. AKASAKA1, Y. YOSHIDA1
1
Department of Biomaterials and Bioengineering, Hokkaido University, Sapporo, Japan, 2Departmentof Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan, 3Clinical Researchand Medical Innovation Center Research and Development Division, Hokkaido University Hospital,
Sapporo, Japan, , 4Department of Oral Diagnosis and Medicine, Hokkaido University, Sapporo, Japan

Dental Materials 4: Adhesion
017: Bond Strengths of 4-META/MMA-TBB Resin Cement to Root Canal Dentin
T. OZAKI, S. OTAKE, W. KOMADA, K. NOZAKI, Y. YOKOSUKA, H. MIURA
Fixed Prosthodontics, Tokyo Medical and Dental University, Tokyo, Japan

Implantology Research
018: Ceria-stabilized zirconia substrate directly bonds to bone-like hydroxyapatite
crystals at nano-scale

M.M. SAITO, Y. YAMAKOSHI
Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University,
Yokohama, Japan

Microbiology/Immunology
019: The Relationship Among Child Dental Caries, Microbiome and Parental Awareness.
Y. KURIYAMA1, E. MIYAMOTO2, M. OHARA2, N. YASUDA1, Y. SHIMIZU1, S. OKAMURA1
R&D, Sunstar Inc., Hyogo, Japan, 2Sunstar Foundation, Osaka, Japan

1020: Involvement of Human Salivary Protein-derived Peptides-specific SIgA In Oral P.
gingivalis Colonization.

K. KOYANAGI1, K. KATAOKA1,2, S. YANAGISAWA2, T. MIYAKE1
Department of Preventive and Community Dentistry, Osaka Dental University, Osaka, Japan,
2
Department of Oral Health Science and Social Welfare, Graduate School of Biomedical Sciences,
Tokushima University, Tokushima, Japan

1021: The Composition and Structure of Oral Microbiome: 16S rRNA Gene Analysis on
Saliva, Plaque, Infected Root Canals obtained from a Patient

K. YAMAKI1, T. TAMAHARA2, J. WASHIO3, T. SATO4
Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry,
Sendai, Japan, 2Department of Community Medical Supports, Tohoku Medical Megabank
Organization, Tohoku University, Sendai, Japan, 3Division of Oral Ecology and Biochemistry, Tohoku
University Graduate School of Dentistry, Sendai, Japan, 4Division of Clinical Chemistry, Niigata
University Graduate School of Health Sciences, Niigata, Japan1

─ 13 ─

Mineralized Tissue022: Bone apatite-clusters in intramembranous and endochondral ossification show
distinct morphologies

E. HARA, M. OKADA, T. MATSUMOTO
Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry andPharmaceutical Sciences, Okayama, Japan

023: A Materials Science-based Re-evaluation of Initial Tooth Mineralization

R. ANADA1, E. HARA1, N. NAGAOKA3, M. OKADA1, H. KAMIOKA2, T. MATSUMOTO1
Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry andPharmaceutical Sciences, Okayama, Japan, 2Department of Orthodontics, Okayama University
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan, 3Advanced
Research Center for Oral and Craniofacial Sciences, Okayama University, Dental School, Okayama,Japan
1

024: Effects of TGF-β isoforms on ameloblasts

Y. MIYAKAWA1, Y. YAMAKOSHI2, Y. ASADA1
1
Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, Yokohama, Japan,
2
Department of Biochemistry and Molecular Biology, Tsurumi University School of Dental Medicine,
Yokohama, Japan

Neuroscience
025: Decreased expression of Neprilysin induced by P. gingivalis-LPS in mouse
hippocampus

T. MORIKAWA1, O. UEHARA2, D. PAUDEL1, K. YOSHIDA1, J. SATO1, Y. ABIKO1
1Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology,
School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 2Division of Disease
Control and Molecular Epidemiology, Department of Oral Growth and Development, School of
Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan

026: Tetrahydrobiopterin pathway: A therapeutic target for neuropathic pain model
S. RAMAN, A. WASKITHO, R. RAJU, M. OSHIMA, Y. MATSUKA
Department of stomatognathic function and occlusal reconstruction, Tokushima University,
Tokushima, Japan

Nutrition Research
027: Fermented dairy food intake reduces risk of tooth loss in a Japanese community

J. MA1, M. FURUTA1, T. TAKESHITA1,2, S. KAGEYAMA1, M. ASAKAWA1, S. SUMA1,
Y. YAMASHITA1
1
Section of Preventive and Public Health Dentistry, Division of Oral Health, Growth andDevelopment, Faculty of Dental Science, Kyushu University, Fukuoka, Japan, 2OBT Research Center,
Faculty of Dental Science, Kyushu University, Fukuoka, Japan

Oral & Maxillofacial Surgery Research
028: Two opposite effects of desmoglein 3 expression on the growth of oral squamous cell
carcinoma between anchorage-dependent and -independent conditions
J. INADA1, M. MINABE1, Y. AKIYAMA1, K. HIGA2, T. TACHIKAWA3, S. TAKAHASHI4,
M. KOUNO4, T. NOMURA1

─ 14 ─

Department of Oral Oncology, Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan,
Cornea Center Eye bank, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan, 3Divisionof Molecular Diagnosis and Cancer Prevention, Saitama Cancer Center, Saitama, Japan, 4Departmentof Dermatology, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan
1
2

Oral Health Research029: Associations between dental caries and Helicobacter pylori infection: A crosssectional pilot study
K. IWAI, T. AZUMA, T. YONENAGA, T. TOMOFUJI
Department of Community Oral Health, School of Dentistry, Asahi University, Gifu, Japan

030: Application and Investigation of Total Adenylate(ATP, ADP, AMP) Hygiene Tests for
Oral Health Monitoring
B. ALTANKHISHIG, M. YASUHIRO, M. KHATUN, T. SAITODivision of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of
Dentistry, Health Science University of Hokkaido, Hokkaido, Japan

Oral Medicine & Pathology Research
031: Chemical direct convesion of human fibroblasts into functional osteoblastic cells
K. YAMAMOTO1,2, T. YAMAMOTO1, T. KISHIDA2, O. MAZDA2, N. KANAMURA1
1
Department of Dental medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan,
2
Department of Immunology, Kyoto Prefectural University of Medicine, Kyoto, Japan

Orthodontics Research032: Applicability of neutral hypochlorous acid water for cleaning fixed orthodontic
appliances

Y. NAGAMATSU, H. IKEDA, H. SHIMIZU
Division of Biomaterials, Department of Oral Functions, Kyushu Dental University, Kitakyushu,
Japan

033: Long Term Effects on Root Resorption: Conventional vs Mini-Screw Assisted Rapid
Palatal Expansion
S. Mehta1, S. A. Arqub2, M. Lagravere3, C. Kuo2, A. Tadinada2, M. Upadhyay2, S. Yadav2
1
Developmental Sciences/Orthodontics, Marquette University School of Dentistry, Milwaukee,
Wisconsin, UNITED STATES, 2University of Connecticut, Farmington, Connecticut, UNITEDSTATES, 3University of Alberta, Edmonton, Alberta, CANADA

Pediatric Oral Health Research034: Identification and characterization of von Willebrand factor D and EGF domain as anovel extracellular matrix protein in teeth

K. IWATA1, K. KAWARABAYASHI2, K. YOSHIZAKI3, A. SUGIMOTO 1, S. FUKUMOTO4,5,
T. IWAMOTO1
1
The Department of Pediatric dentistry and special needs dentistry, Tokyo medical and dental
University , Tokyo, Japan , 2Department of Pediatric Dentistry, Institute of Biomedical Sciences,
Tokushima University Graduate School, Tokushima, Japan, 3Section of Orthodontics, Division of Oral
Health, Growth and Development.Faculty of Dental Science, Kyushu University, Fukuoka, Japan ,4
Department of Pediatric Dentistry, Graduate School of Dental Science, Kyushu University, Fukuoka,
Japan, 5Division of Pediatric Dentistry, Department of Oral Health and Development Sciences,─ 15 ─

Tohoku University Graduate School of Dentistry, Sendai, Japan

Periodontal Research-Therapy
035: High-fat diet-induced XAF1 exacerbates diabetes by promoting pancreatic β-cell
apoptosis

Y. NISHIMURA1, M. IWASHITA1, M. HAYASHI1, T. SHINJO1, T. ZEZE1, A. YAMASHITA1,
T. FUKUDA1, T. SANUI1, T. SANO2, F. NISHIMURA1
1
Department of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan, 2Department of Cell Biology and Pharmacology, Faculty of Dental
Science, Kyushu University, Fukuoka, Japan

036: Inhibitory effects of β-Glycyrrhetinic acid on bacterial growth and biofilm formation
by supragingival plaque commensals

N. DEWAKE1, X. MA1, K. SATO2, S. NAKATSU2, K. YOSHIMURA2, Y. ESHITA2, H. FUJINAKA2,
Y. YANO2, A. YOSHIDA3, N. YOSHINARI1
1
Department of Periodontology, Faculty of Dentistry, Matsumoto Dental University, Shiojiri, Japan,
2
Personal Health Care Product Research, Kao Corporation, Tokyo, Japan, 3Department of Oral
Microbiology, Faculty of Dentistry, Matsumoto Dental University, Shiojiri, Japan

Periodontal Research-Pathogenesis
037: Insulin resistance in vascular endothelial cells contributes to the exacerbation of
periodontitis via dysregulation of inflammation-induced VCAM-1 expression

T. ZEZE, T. SHINJO, Y. NISHIMURA, K. SATO, M. IMAGAWA, M. IWASHITA, A. YAMASHITA,
F. NISHIMURA
Department of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan

038: Dipotassium Glycyrrhizate inhibits LPS-induced alteration of cytoskeleton in
gingival fibroblasts
T. YANAGISAWA, A. YOSHIDA, M. INAGAKI, S. SATONIPPON ZETTOC CO., LTD, Sagamihara, Japan

Periodontal Research-Diagnosis/Epidemiology
039: The loss of IκBζ accelerates dentin formation and matrix gene expression

H. YUAN1, S. SUZUKI1, H. TERUI2, S. HIRATA-TSUCHIYA3, E. NEMOTO1, K. YAMASAKI2,
M. SAITO4, H. SHIBA3, S. AIBA2, S. YAMADA1
1
The Department of Periodontology and Endodontology, Tohoku University school of Dentistry,
Sendai, Japan, 2Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai,
Japan, 3Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences,
Hiroshima University, Hiroshima, Japan, 4Department of Restorative Dentistry, Tohoku University
Graduate School of Dentistry, Sendai, Japan

Periodontal Research-Pathogenesis
040: Analysis of periodontopathic bacteria related to early primary tooth loss

Y. MIYAI, S. NAKA, M. MATSUMOTO-NAKANOOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan─ 16 ─

Periodontal Research-Therapy
041: Hepatocyte growth factor shows antifibrotic effect on nifedipine-induced gingival
overgrowth in vitro model

K. YAMAZAKI1, H. IGARASHI-TAKEUCHI1,2, Y. NUMABE1
1
Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University,
Tokyo, Japan, 2 Core Research Facilities for Basic Science, Research Center for Medical Science, The
Jikei University School of Medicine, Tokyo, Japan

042: Personalized medicine of oral implant therapy for otorhinolaryngological high-riskpatients.

K. TAKAHASHI1, K. YAMAZAKI1, M. YAMAZAKI2, Y. BABA3
1
Division of Periodontics, Department of Conservative Dentistry, Ohu University School of Dentistry,
Koriyama, Japan, 2Ohu University Dental Hospital, Koriyama, Japan, 3Ohu University Hospital
Otorhinolaryngology, Koriyama, Japan

Periodontal Research-Pathogenesis
043: Abnormal expression of SGLT2 in the kidney of a P. gingivalis LPS-induced diabetic
nephropathy mouse model
K. KAJIWARA1, S. TAMAOKI1, Y. SAWA2
1
Division of orthodontics, department of oral growth & development, Fukuoka Dental College,
Fukuoka, Japan, 2Deparment of Oral Function & Anatomy, Okayama University Graduate School of
Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

Pharmacology/Therapeutics/Toxicology
044: Anti-inflammatory and Anti-oxidative Effects of Febuxostat on Periodontitis Rats
Model

N. NESSA1,2, M. KOBARA2, T. ADACHI1, T. YAMAMOTO1, T. NAKATA2, N. KANAMURA1
1
Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan, 2Pathological Science,
Department of Clinical Pharmacology, Kyoto Pharmaceutical University, Kyoto, Japan

045: Effects of smoking and smoking cessation on human gingival and lung fibroblasts

H. TAKEUCHI-IGARASHI1,2, T. TACHIBANA2, Y. MANOME2, Y. NUMABE1
1
Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University,
Tokyo, Japan, 2Core Research Facilities for Basic Science, Research Center for Medical Science, The
Jikei University School of Medicine, Tokyo, Japan

Prosthodontics Research046: Profiles of Mastication Predominance and Masticatory Performance in Kennedy
Class I Patients

K. KINOSHITA1, K. OKI2, Y. OGINO2, Y. TSUKIYAMA3, Y. AYUKAWA1, K. KOYANO4
1
Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental
Science, Kyushu University, Fukuoka, Japan, 2Section of Fixed Prosthodontics, Division of Oral
Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan, 3Section of Dental
Education, Division of Oral Biological Sciences, Faculty of Dental Science, Kyushu University,
Fukuoka, Japan, 4Division of Advanced Dental Devices and Therapeutics, Faculty of Dental Science,
Kyushu University, Fukuoka, Japan─ 17 ─

047: Bacterial flora evaluation using DNA array chip and real-time PCR
Y. KADOTA, E. ARIMA, S. HOTTA, F. FUSEJIMA
GC CORPORATION, Tokyo, Japan

048: Effect of Glass Fiber Sleeve for Reinforcement of Flared Root

S. YOSHIMATSU, W. KOMADA, R. NEMOTO, S. OISHI, R. TSUKAHARA, S. OMORI,
K. NOZAKI, H. MIURA
Fixed Prosthodontics, Tokyo Medical and Dental University, Tokyo, Japan

049: The Influence of Zirconia Tube for Stress in Endodontically-Treated Molar

S. OISHI, W. KOMADA, D. KONDO, R. TSUKAHARA, S. YOSHIMATSU, Y. YOKOSUKA,S. OMORI, K. NOZAKI, H. MIURA
Fixed Prosthodontics, Tokyo Medical and Dental University, Tokyo, Japan

Pulp Biology & Regeneration Research
050: New mechanism of odontoblast differentiation

H. NAKAZATO1, S. ONODERA2, N. AIDA2, T. AZUMA2, M. FURUSAWA1
1
Department of Endodontics, Tokyo Dental College, Tokyo, Japan, 2Department of Biochemistry,
Tokyo Dental College, Tokyo, Japan

051: Evaluation of Inflammatory Changes During Wound Healing Process in CariesStimulated Dental Pulp

H. HUANG, M. OKAMOTO, M. WATANABE, S. MATSUMOTO, K. MORIYAMA, S. KOMICHI,
Y. TAKAHASHI, M. HAYASHI
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of
Dentistry, Osaka, Japan

052: Induction of dental pulp cells into periodontal ligament-like cells using epigenetics

S. TAKAHASHI1, K. YOSHIDA1, D. PAUDEL1, D. ARIWANSA1, A. KHURELCHULUUN2,
A. ONISHI1, T. MORIKAWA1, D. HIRAKI3, F. HARADA2, O. UEHARA4, J. SATOU1,
H. NAGAYASU2, Y. ABIKO1
1Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of
Hokkaido, Hokkaido, Japan, 2Division of Oral Medicine and Pathology, School of Dentistry, Health
Sciences University of Hokkaido, Hokkaido, Japan, 3Division of Oral and Maxillofacial Surgery,
School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 4Division ofReconstructive Surgery for Oral and Maxillofacial Region, School of Dentistry, Health Sciences
University of Hokkaido, Hokkaido, Japan, 5Division of Disease Control and Molecular Epidemiology,
School of Dentistry, Health Sciences University of Hokkaido

053: Dentin-pulp complex tissue regeneration by three-dimensional cell sheet engineering
H. YAN1, M. OSHIMA1, R. RAJU2, S. RAMAN1, A. WASKITHO1, K. OKURA1, Y. MATSUKA1
1
Department of Stomatognathic Function and Occlusal Reconstruction, University of Tokushima,
Tokushima, Japan, 2Department of Oral Disease Research, National Center for Geriatrics andGerontology, Obu, Nagoya, Japan

Salivary Research054: Effect of psychological stress on oral microbiome: A metagenomic and bioinformatic
analyses

D. PAUDEL1, Y. KURAMITSU2, O. UEHARA3, T. MORIKAWA1, T. KITAGAWA1, K. YOSHIDA1,
Y. ABIKO1

─ 18 ─

Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of
Hokkaido, Hokkaido, Japan, 2Advanced Research Promotion Center, Health Sciences University of
Hokkaido, Japan, 3Division of Disease Control and Molecular Epidemiology, School of Dentistry,
Health Sciences University of Hokkaido, Japan1

Stem Cell Biology Research
055: Exosomal miR-1260b derived from TNF-α-treated hGMSCs inhibits periodontalbone loss by targeting ATF6β-mediated regulation of RANKLC. HAYASHI, Y. WATANABE, K. KAWAKAMI, M. TOYODA, Y. NAKAO, K. YOTSUMOTO,
H. YAMATO, T. SHINJO, T. SANUI, T. FUKUDA, F. NISHIMURA
Departmen of Periodontology, Division of Oral Rehabilitation, Faculty of Dental science, Kyushu
University, Fukuoka, Japan

056: Exosomes derived from GMSCs stimulataed with TNF-a and IFN-a promote M2
macrophage polarization via enhanced CD73 and CD5L expression

Y. WATANABE, C. HAYASHI, K. KAWAKAMI, M. TOYODA, Y. NAKAO, K. YOTSUMOTO,
H. YAMATO, T. SHINJO, T. SANUI, F. NISHIMURA
Departmen of Periodontology, Division of Oral Rehabilitation, Faculty of Dental science, Kyushu
University, Fukuoka, Japan

057: Characterization of neural crest-derived cells for application in bone regenerative
medicine
K. SHINOMIYA, A. YAMADA, R. KAMIJO
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan

058: The effect of JNK inhibition on the osteoblastic differentiation of periodontal
ligament stem cells and the regeneration of periodontal tissue in vivo

H. KANEKO1, D. HASEGAWA2, T. ITOYAMA2, S. YOSHIDA2, A. TOMOKIYO2, S. HAMANO1,3,
H. SUGII1, H. MAEDA1,2
1
Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan., 2Division of Endodontology, Kyushu University Hospital, Fukuoka,
Japan, 3 OBT Research Center, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

059: Relationship between age-related changes in MSC functions and periodontal tissues
destruction in ligature induced periodontitis mouse model

K. AUNG, K. AKIYAMA, T. KUBOKI
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate Schoolof Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

060: MSC conditioned medium from older mice affects its osteogenic differentiation
capacity

J. ZHANG, K. AKIYAMA, K. MAEKAWA, T. KUBOKI
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate Schoolof Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

061: Extracellular vesicles-mediated novel therapeutic mechanism of deciduous toot pulp
stem cell-based therapy for systemic lupus erythematosus
S. SONODA, T. YAMAZA
Department of Molecular Cell Biology and Oral Anatomy, Division of Oral Biological Sciences,
Kyushu University Graduate School of Dental Science, Fukuoka, Japan─ 19 ─

SPECIAL LECTURES

─ 21 ─

Special Lecture
Sunday, October 24th, 10:30-11:30
Video distribution
SL
Past, Present and Future of Mucosal Vaccine:
Vital Contributions Made by Dentistry

Hiroshi Kiyono, D.D.S., Ph.D.

IMSUT Distinguished Professor
Division of Mucosal Immunology, IMSUT Distinguished Professor Unit
The Institute of Medical Science, The University of Tokyo
Professor
Mucosal Immunology and Allergy Therapeutics
Institute for Global Prominent Research
Graduate School of Medicine, Chiba University
Professor of Medicine
CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines
Division of Gastroenterology, Department of Medicine, School of Medicine/andDirector, CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines (cMAV)
University of California, San Diego

Considering the global health, vaccine is certainly one of the key strategies for achieving
our common goal of healthy society. Through the current and unfortunate SARS-CoV-2
pandemic situation, all of us learned again the value of vaccines. Admirable heroic
efforts have been made to develop the first-generation of injection-type vaccines for
SARS-CoV-2, and thus for example mRNA-based injectable vaccines are being
administered in the different continents. It should be noted that injected vaccines
effectively induce systemic immunity (e.g., serum IgG) to prevent severe disease, but
not mucosal IgA that prevents pathogen invasion of the airway mucosa. The aerodigestive tract, beginning from oral and nasal cavities are covered by mucosal
epithelium. These mucosal surfaces are continuously exposed to environmental
antigens including pathogens and allergens, and thus equipped with the mucosal
immune system for the initial recognition of their pathogenicity and subsequent
induction of pathogen-specific immunity. At the dawn of our scientific effort for thedemonstration of the mucosal immune system, dental science demonstrated
foreseeability and contributed to building the scientific foundation for the importanceof the mucosal immune system and its application for the control of infectious diseases.
Thus, the development of mucosal vaccines for the prevention of dental caries was apart of novel approaches which contributed for initiating the scientific area of mucosal
immunology. Since then, mucosal immunology and vaccines have gained scientificjourney to become one of the major entities of Immunology. Through this presentation,
I wish to introduce the past and current efforts and future directions for thedevelopment of mucosal vaccines, focusing on our nanogel-based nasal vaccine andrice-based oral vaccine (MucoRice), for the prevention of aero-digestive tract associatedinfectious diseases by the interdisciplinary blending of mucosal immunology withbiomaterial engineering and agriculture science, respectively.

─ 22 ─

Hiroshi Kiyono, D.D.S., Ph.D.
IMSUT Distinguished Professor
Division of Mucosal Immunology, IMSUT Distinguished Professor Unit
The Institute of Medical Science, The University of Tokyo (IMSUT)

Special Lectures

Brief CV

Professor
Mucosal Immunology and Allergy Therapeutics
Institute for Global Prominent Research, Graduate School of Medicine, Chiba University

In the late 1970s, Dr. Kiyono has started his significant research career in the launch of the “Mucosal
Immunology.” He consistently played an important role as a pioneer in this research area with his excellent
mentor, colleagues and collaborators. In particular, his research team explored in 1) Peyer’s patches (PPs), one ofcore members of Gut-Associated Lymphoid Tissues (GALT) possess antigen-presenting cells and IgA isotype
specific helper T cells (now classified in Th2 cells) for the induction of antigen-specific mucosal immunity; 2)intestinal mucosa, epithelial, mesenchymal, and immune cells form a multi-ecological system in concert with thegut microbiota to create and maintain homeostasis; and 3) the respiratory mucosal immune system with its
unique immunobiological characteristics. His research team has also actively facilitated the development of thenext-generation vaccines, the oral and nasal vaccines, which now are in critical pre-clinical and clinical trial
stages.

[Education]
1983
1977

Ph.D., University of Alabama at Birmingham Medical Center (UAB), USA
D.D.S., Nihon University School of Dentistry at Matsudo, Japan

2018-Present

2015-Present
2011-20191991-2003
1989-1990
1986-1987
1984-1987

─ 23 ─

Poster Presentation

2011-20152007-2010
2002-2018
1994-2003

IMSUT Distinguished Professor, Department of Mucosal Immunology
IMSUT Distinguished Professor Unit
The Institute of Medical Science, The University of Tokyo (IMSUT)
Professor of Medicine, Division of Gastroenterology
Department of Medicine/
Director, CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines
University of California, San Diego
Professor, Mucosal Immunology and Allergy Therapeutics,
Graduate School of Medicine, Chiba University
Professor and Director
International Research & Development Center for Mucosal Vaccines, IMSUT
Dean, IMSUT
Associate Dean, IMSUT
Professor and Director, Division of Mucosal Immunology, IMSUT
Professor and Chairman, Department of Mucosal Immunology,
Research Institute for Microbial Diseases, Osaka University
Professor, Departments of Oral Biology and Microbiology, UAB
Associate Professor, Departments of Oral Biology and Microbiology, UAB
Visiting Senior Scientist, Max-Planck Institute for Biology
Research and Clinical Assistant Professor, Department of Oral Biology, UAB

Rising Scientist Session[Career]
2018-Present

Symposium II

He is currently the President of the Japanese Society for Immunology and was the former President of JapaneseSociety for Mucosal Immunology and the editorial board member of scientific Journals such as Vaccine, Mucosal
Immunology, Human Vaccines and Frontiers. He himself has a total of 567 publications in peer review journals
and edited a total of 22 books.

Symposium I

Dr. Kiyono’s background as a dentist combined with extensive research experience in the field of Mucosal
Immunology makes him exceptionally well qualified to discuss the current and future directions of mucosal
immunology and mucosal vaccine development. To reflect his scientific contribution, he was listed in ISI Highly
Cited Researchers’ List and received several prestigious awards such as NIH New Investigator Research Award,
NIH Research Career Development Award, The Japanese Society for Vaccinology Takahashi Award and Hideyo
Noguchi Memorial Medical Science Award.

Joint Symposium

Professor of Medicine
Division of Gastroenterology, Department of Medicine, School of Medicine/
Director
CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines
University of California, San DiegoGreeting from IADR President
Sunday, October 24th, 9:00-9:15
Video distribution
G-1

Laureate Professor Eric C. Reynolds AO FICD FTSE FRACDSCentre for Oral Health Research, Melbourne Dental School
The University of Melbourne

Laureate Professor Eric Reynolds AO is Chief Executive Officer and Research Director of
the Oral Health CRC at the Melbourne Dental School, the University of Melbourne. For
16 years until 2015 Eric was Head of the Melbourne Dental School. He has lectured andpublished extensively and has chaired and participated in a wide range of professional
committees and panels. Eric was appointed an Officer of the Order of Australia for his
service to community dental health in 2005. He received the Clunies Ross National
Science and Technology award in 2002 and the Victoria Prize for Science in 2005. In
2011 he received the Distinguished Scientist Award from the International Association
for Dental Research, and in 2015 the Leach Medal for research excellence and the
Global Health Impact Award from the University of Melbourne. In 2016 he received theAward of Merit from the Australian Dental Association and in 2017 the Prime Minister’sPrize for Science Innovation. In 2019 he was elected Vice-President of the International
Association for Dental Research and became President in July this year. In 2020 hereceived the European Organisation for Caries Research Prize for outstanding scientificcontribution and the American Academy of Periodontology Clinical Research Award.

─ 24 ─

Special Lectures

Joint Symposium

Symposium I

Symposium II

Rising Scientist Session

Poster Presentation

─ 25 ─

KADR President Lecture
Sunday, October 24th, 9:15-10:15
Video distribution
KL
Esthetic & functional oral rehabilitation : 4 control
factors

Professor, Kung-Rock KWON, DMD, MSD, PhD
Dept. of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul,Korea

Our purpose is to restore the vital function and characteristic beauty of the masticatory
apparatus by an intelligent application of the basic requirements such as anatomic,
physiologic, mechanical, hygienic, and esthetic which show that no superstructure canbe more enduring than its foundation.
How is this achieved? Before going into treatment, precise diagnosis is very important.
tentative treatment plan is made based on the list of problems and diagnosis. reversible
treatment is chosen first to give final treatment through trial-and-error process.
What factors do we think can be controlled?
1. Optimum functional joint position is a physiologic position where condyle headis braced with disk and positioned antero-speriorly in the articular eminence.
2. CR and physiological position of condyle with maximum intercuspation should
be considered.
3. Vertical dimension of occlusion is most important factor to consider.
4. anterior guide angle must be adjusted to maintain the determined VD, andocclusal stability.
This lecture will be focusing on vertical dimension in general, method to test and decide
the vertical dimension of the patient and the treatment and follow-up check of thevertical height loss with prosthetic perspective.

─ 26 ─

Kwon, Kung-Rock ( DMD, MSD, PhD, MBA, FICD )Education
2007-2010
1993-1995
1988-1990
1981-1987

MBA
PhD
MS
DMD

Kyung Hee Univ. Graduate School of Business, Seoul, Korea
Kyung Hee Univ. School of Dentistry, Seoul, Korea
Kyung Hee Univ. School of Dentistry
Kyung Hee Univ. School of Dentistry

Joint Symposium

Certified Specialty : Prosthodontics
Address : Dept. of Prosthodontics, School of Dentistry, Kyung Hee University,
Kyunghee dae-ro 26, Seoul 02447, Korea
E-mail address : krkwon@khu.ac.kr
Position : Professor

Special Lectures

Brief CV

Major Careers
2018-2020
Dean
2016-2017
Vice Dean
2002-2003
Visiting Professor
1996-1998
Visiting Professor
1993-1996
Fellow
1988-1990
Resident
1999-present Professor

Kyung Hee Univ. School of Dentistry, Seoul, Korea
Kyung Hee Univ. School of Dentistry
Harvard Univ. School of Dentistry, USA
Geneva Univ. School of Dentistry, Switzland
Kyung Hee Univ. Dental Hospital
Kyung Hee Univ. Dental Hospital
Kyung Hee Univ. School of Dentistry

Symposium I

Academic Societies2021-present President
2020-present President
2020-present President elected2014-2020
Secretary
2018-2020
Vice President
2017-2019
President
2015-Present Vice President

The Korean Academy of Implant Dentistry
The Korean Academy of Prosthodontics
The Korean Academy of Dental Research
The Korean Academy of Dental Sciences
The Korean Dental Hospital Association
The Korean Academy of Sports Dentistry
The Korean Officials Dental Association

Symposium II
Poster Presentation

─ 27 ─

Rising Scientist Session

Research papers(SCI(E) : Recent 2yrs
- A novel retentive type of dental implant prosthesis: marginal fitness of the cementless
double crown type implant prosthesis evaluated by bacterial penetration andviability.J Adv.Prosthodont 2020;12:233-8- Setting the Sagittal Condylar Inclination on a Virtual Articulator Using Intraoral Scan ofProtrusive Interocclusal Position and Cone Beam Computed Tomography.
Journal of Prosthodontics 29 (2020) 185–189- Virtual diagnostics and guided tooth preparation for the minimally invasive rehabilitation ofa patient with extensive tooth wear: A validation of a digital workflow. J
Prosthet Dent 2020;123:20-6- A CAD-CAM device for preparing guide planes for removable partial dentures: A dental
technique J Prosthet Dent 2019;122:10-13- Combining Conventional Impressions and Intraoral Scans: A Technique for the Treatment ofComplete Denture Patients with Flabby Tissue. Journal of Prosthodontics 28(2019) 592-595- Comparing accuracy of denture bases fabricated by injection molding, CAD/CAM milling,
and rapid prototyping method. J Adv Prosthodont 2019;11:55-64- Precise Reproduction of Soft Tissue Structure around the Pontic Area Using Computer-Aided
Design and Manufacturing. Journal of Prosthodontics 28 (2019) 216–218- Effects of cementless fixation of implant prosthesis: A finite element study. The Journal ofAdvanced of Prosthodont 2019;11:341-349

SYMPOSIUM
The 1st Joint Symposium of KADR and JADR :Regenerative medicine/Dentistry

Symposium I :Japan-Originated Next Generation Biomedical Materials

Symposium II:
Current progress of Stem Cell Biology
and Regenerative Medicine in Dental Science

Rising Scientist Session :Periodontal Medicine

─ 29 ─

The 1st Joint Symposium of KADR and JADR
Sunday, October 24th, 13:30-15:30
Video distribution and Live Session
JS-1
Comprehensive analysis for the genes associated withepithelial-mesenchymal interactions in tooth

Keigo Yoshizaki, D.D.S., Ph. D.
Department of Orthodonitcs and Dentofacial Orthopedics, Faculty of Dental
Science, Kyushu University

Epithelial–mesenchymal interaction plays critical roles for the development of organs
such as tooth, lung, salivary gland, kidney and hair. During this process, epithelial
thickening and budding into mesenchyme are common phenomenon, while the decisionof cell fate is made by the specific transcriptional controls of individual organs. In thisstudy, specific transcriptional start sites (TSS) of each organ were explored by CAGE (Cap
Analysis of Gene Expression) analysis. We found that commonly expressed TSS weremainly detected in the epithelia, whereas specific TSS were expressed in mesenchymein each organ. Furthermore, we identified a tooth specific TSS detected in thechromosome 15qD1 region, which codes microRNA-875 (miR875). MiR875 is specifically
expressed in dental mesenchymal cells during the tooth germ formation. Furthermore,
PRRX1 binds to the miR875 promoter region and enhances the expression of miR875. To
assess the role of miR875 in dental mesenchyme during tooth development, wetransfected mimic miR875 into mouse dental pulp (mDP) cells, which showed that cell
migration toward dental epithelial cells was significantly induced by miR875 via the
PDGF signaling pathway. Further, we also demonstrate that miR875 induced cell
migration by inhibiting PTEN and STAT1, which are regulated by miR875 in posttranscriptional regulation. In summary, our findings indicate that tooth specific miR875
has important roles in early tooth development, especially cell condensation ofmesenchymal cells around invaginated dental epithelium and induction of epithelialmesenchymal interaction.

─ 30 ─

Keigo Yoshizaki
Section of orthodontics and Dentofacial Orthopedics, Division of Oral Health, Growth andDevelopment, Department of Dental Science, Kyushu University

Research & Professional Experience:
2008-2009
Resident, Kyushu University Hospital, Fukuoka, Japan
2009-2011
Research Fellow, the Japan Society for the Promotion of Science (JSPS)
2010-2013
Postdoctoral Fellow, LCDB, NIDCR, NIH, Bethesda, MD, USA
2013Assistant professor, Kyushu University, Japan

Travel Award, 15th The Kyushu Orthodontic Society

Symposium I

Award:
2019

Joint Symposium

Education
Undergraduate 1998-2004 Kyushu University School of Dentistry, Fukuoka, Japan, D.D.S.
Graduate 2004-2008 Kyushu University, Dental Science, Fukuoka, Japan, Ph.D.

Special Lectures

Brief CV

Symposium II
Rising Scientist Session
Poster Presentation

─ 31 ─

The 1st Joint Symposium of KADR and JADR
Sunday, October 24th, 13:30-15:30
Video Distribution and Live Session
JS-2
Periodontal regeneration by BMP7-overexpressing bonemarrow mesenchymal stem cells in a ligature-induced
periodontitis rat model
Jeong-Ho Yun, B.S., D.D.S., M.S.D., Ph. D.
Department of Periodontology, Jeonbuk National University College ofDentistry

Periodontal disease remains a challenge in clinical regenerative dentistry. The
complexity of the periodontium, consisting of cementum, periodontal ligament, alveolarbone, and gingiva, adds to the difficulties for regeneration. Although various
regenerative techniques have been evaluated, conventional regenerative treatment forperiodontal wound is not yet reliable to regenerate the impaired tooth supporting
structures. To achieve an advanced reconstruction of lost periodontal tissue, a newtechnique using additional signaling molecules and cells was introduced, namely tissue
engineering.
In the present study, we aimed to evaluate the effect of bone morphogenetic protein 7(BMP7) with bone marrow mensenchymal stem cell (BM-MSC) transplantation in theregenerative therapy for periodontitis. BMP7 is one of the BMP growth factor family
members showing excellent bone forming ability, however its short half-life needs toovercome for clinical application. Therefore, BMP7-expressing immortalized BM-MSCs(BMP7-eBMSCs) were established and they showed superior osteogenic differentiation
potential when subcutaneously transplanted with biphasic calcium phosphate scaffold
on immunocompromised mice. Furthermore, efficacy of BMP7-eBMSCs transplantation
was evaluated for periodontal tissue regeneration in rats with ligature-induced
periodontitis. As expected, the damaged alveolar bone in periodontitis was regenerated
in BMP7-eBMSC transplantation group both in 2-dimensional and 3-dimensional
analyses of micro-CT scan and histology. Interestingly, the amount of regenerated
alveolar bone in BMP7-eBMSCs transplantation group was comparable to that in thetransplantation of human periodontal ligament stem cells.
Taken together, our results suggest that BMP7-eBMSCs could be one of the feasible
treatment options for the regeneration of periodontal tissue damaged by periodontitis.

─ 32 ─

Jeong-Ho YUN
Department of Periodontology, Jeonbuk National University College of Dentistry

Symposium I
Symposium II

Research & Professional Experience:
2001-2004
Internship and Residency, Department of Periodontics, Yonsei University
Dental Hospital
2004-2005
Research Fellow, Department of Periodontology, Yonsei University College ofDentistry
2005-2010
Instructor / Assistant professor, Department of Dentistry, Kwandong University
College of Medicine, Goyang, Korea
2011-2015
Assistant Professor / Associate professor, Division of Periodontology,
Department of Dentistry, Inha University School of Medicine, Incheon, Korea
2018-2018
Visiting Researcher, Tokyo Women’s Medical University ABMES (Institute ofAdvanced BioMedical Engineering and Science), Tokyo, Japan
2019-2020
Visiting Scholar, Department of Biomedical Engineering, University of Southern
California, LA, USA
2015Associate Professor / Professor & Chairman, Department of Periodontology,
Jeonbuk National University College of Dentistry, Jeonju, Korea

Joint Symposium

Education
Undergraduate 1991-1997 Life Science, Pohang University of Science and Technology(POSTECH), Pohang, Korea, B.S.
1997-2001 Yonsei University College of Dentistry, Seoul, Korea, D.D.S.
Graduate 2002-2004 Graduate School of Dentistry, Yonsei University, Seoul, Korea, M.S.D.
2004-2007 Graduate School of Dentistry, Yonsei University, Seoul, Korea, Ph.D.

Special Lectures

Brief CV

Rising Scientist Session
Poster Presentation

─ 33 ─

The 1st Joint Symposium of KADR and JADR
Sunday, October 24, 13:30-15:30
Video distribution and Live Session
JS-3
Exacerbation mechanism of alveolar bone destruction
in apical periodontitis caused by inflammatory bowel
disease.
Masahiro Saito, D.D.S., Ph. D.
Department of Ecological Dentistry Division of Operative Dentistry Tohoku
University Graduate School of Dentistry

Apical periodontitis (AP) is an acute or chronic inflammatory disease caused by complex
interactions between infected root canal and host immune system. Although reducing
the pathogen load from the root canal typically prevents reinfection and leads tohealing of the AP, the complex root canal system and host immune system sometimes
interfere with these processes resulting in therapy-resistant AP lesions that induce
continuous apical bone tissue destruction. Inflammatory bowel disease (IBD) is causesby breakdown by intestinal immunity and affect not only in colon function but also in
exacerbate systemic immune system such as causing iritis, uveitis, ankylosing spondylitis
and arthritis. Similar to these event, IBD enhanced inflammation of the periapical area,and bone destruction. Thus, a fuller understanding of the relationship of AP and IBD
may identify potential new therapeutic targets for therapy-resistance case.
We have previously established mice AP model and shown that macrophage-derived
CXCL9, which acts through CXCR3, is recruited by progressed AP. The inhibition of CXCL9
by a CXCR3 antagonist reduced the lesion size in a mouse AP model with decreasing IL1β, IL-6 and TNFα expression. The treatment of peritoneal macrophages with CXCL9 and
LPS induced the transmigration and upregulation of osteoclastogenic cytokines such as
IL-1β, IL-6 and matrix metalloprotease 2, a marker of activated macrophages. Thus,CXCL9-CXCR3 axis plays a crucial role in the development of AP, mediated by the
migration and activation of macrophages for periapical tissue destruction. From thesedata we showed that knowledge of the principal factors involved in the progression ofAP, and the identification of related inflammatory markers, may help to establish newtherapeutic strategies.
In the present study, we report mice AP-IBD model to investigate effect of IBD on
alveolar bone destruction induced by AP and provide new evidences how IBD accelerate
alveolar bone destruction in this model.

─ 34 ─

Masahiro Saito
Tohoku University Graduate School of Dentistry Division of Operative Dentistry, Departmentof Ecological Dentistry

Symposium I

Research & Professional Experience:
1988-1994
Assistant professor, Department of Operative dentistry, Kanagawa Dental
College
1994-1996
Visiting Fellow, School of Medicine, Department of Pathology, University ofWashington, Seattle, WA
1996-2002
Assistant professor, Department of Operative Dentistry and Endodontics,
Kanagawa Dental College
2002-2006
Lecturer, Department of Operative Dentistry and Endodontics, Kanagawa
Dental College
2006- 2009 Lecturer, Department of Molecular and Cellular Biochemistry, Osaka University
Graduate School of Dentistry2009-2013
Associate professor, Faculty of Biological Science, Tokyo University of Science
2013-present Professor, Division of Operative Dentistry, Department of Ecological Dentistry,
Tohoku University, Graduate School of Dentistry

Joint Symposium

Education
Undergraduate 1982-1988 Kanagawa Dental College, Yokosuka, Japan, D.D.S.
Graduate 1994 Ph.D., Kanagawa Dental College, Japan, Ph.D.

Special Lectures

Brief CV

Symposium II
Rising Scientist Session
Poster Presentation

─ 35 ─

The 1st Joint Symposium of KADR and JADR
Sunday, October 24th, 13:30-15:30
Video distribution and Live Session
JS-4
Implant Supported Overdentures for the Elderly
Edentulous Patients

Professor, Kung-Rock KWON, DMD, MSD, PhD
Dept. of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul,
Korea

Edentulous patients with a severely resorbed mandible represent a significant healthcare problem in the growing elderly population. Despite adequate denture fabrication,
it is not possible in many instances to achieve optimal retention and stability in theconventional mandibular denture. This may be caused by poor jaw and ridge
relationship, psychologic conditions, reduced neuromuscular coordination, inadequate
quality and quantity of available bone and alveolar mucosa. To achieve increased
retention, pre-prosthetic surgery to augment the alveolar ridge or increase thevestibular depth may be needed. But the outcome of these pre-prosthetic surgery areless favorable than that of the implant retained overdenture.
Zarb et al. described that the quality of life of elderly edentulous patients can be
improved using inter-foraminal implants to resolve the edentulism. There also was aconsensus statement at McGill University of mandibular two-implant overdenture as
the first choice standard of care for edentulous patients. Overdenture retained by two
or four implants has shown to be a highly successful prosthetic treatment similar to the
fixed implant denture.
A variety of attachment systems have been used to retain overdentures, generally,
these can be classified as clips-and–bars, balls, and magnets. The selection of an
attachment system is mainly related to the personal choice of the practitioner and/or
laboratory responsible, based on experience and training. The clinical trial has
compared clinical outcomes.
According to the conventional Branemark protocol, periods of 3 months and 6 months
have been recommended as healing times for osseointegrated implants placed in themandible and maxilla, respectively, during which functional loading should be avoided.
However these long healing periods before functional loading are not based on
histologic and experimental results, but are from empirical clinical experience. These
periods can impose not only functional challenges, but also inconvenience of theedentulous state to the patient.
The evaluation of different early loading protocols for un-splinted implants for
mandibular overdentures opposing conventional complete maxillary dentures is best
achieved by comparing groups with different reductions in healing times.
In the case of elderly edentulous patient, treatment should be carried out inconsideration of the patient’s age and general health as well as oral health related
quality of life.

─ 36 ─

Kwon, Kung-Rock (DMD, MSD, PhD, MBA, FICD)
Certified Specialty : Prosthodontics
Address : Dept. of Prosthodontics, School of Dentistry, Kyung Hee University,
Kyunghee dae-ro 26, Seoul 02447, Korea
E-mail address : krkwon@khu.ac.kr
Position : Professor

Kyung Hee Univ. Graduate School of Business, Seoul,
Kyung Hee Univ. School of Dentistry, Seoul, Korea
Kyung Hee Univ. School of Dentistry
Kyung Hee Univ. School of Dentistry

Major Careers
2018-2020
2016-2017
2002-2003
1996-1998
1993-1996
1988-1990
1999-present

DeanVice Dean
Visiting Professor
Visiting Professor
Fellow
Resident
Professor

Kyung Hee Univ. School of Dentistry, Seoul, Korea
Kyung Hee Univ. School of Dentistry
Harvard Univ. School of Dentistry, USA
Geneva Univ. School of Dentistry, Switzland
Kyung Hee Univ. Dental Hospital
Kyung Hee Univ. Dental Hospital
Kyung Hee Univ. School of Dentistry
The Korean Academy of Implant Dentistry
The Korean Academy of Prosthodontics
The Korean Academy of Dental Research
The Korean Academy of Dental Sciences
The Korean Dental Hospital Association
The Korean Academy of Sports Dentistry
The Korean Officials Dental Association

Poster Presentation

─ 37 ─

Rising Scientist Session

Research papers(SCI(E) : Recent 2yrs
- A novel retentive type of dental implant prosthesis: marginal fitness of the cementless
double crown type implant prosthesis evaluated by bacterial penetration andviability.J Adv.Prosthodont 2020;12:233-8- Setting the Sagittal Condylar Inclination on a Virtual Articulator Using Intraoral Scan ofProtrusive Interocclusal Position and Cone Beam Computed Tomography.
Journal of Prosthodontics 29 (2020) 185–189- Virtual diagnostics and guided tooth preparation for the minimally invasive rehabilitation ofa patient with extensive tooth wear: A validation of a digital workflow. J
Prosthet Dent 2020;123:20-6- A CAD-CAM device for preparing guide planes for removable partial dentures: A dental
technique J Prosthet Dent 2019;122:10-13- Combining Conventional Impressions and Intraoral Scans: A Technique for the Treatment ofComplete Denture Patients with Flabby Tissue. Journal of Prosthodontics 28(2019) 592-595- Comparing accuracy of denture bases fabricated by injection molding, CAD/CAM milling,
and rapid prototyping method. J Adv Prosthodont 2019;11:55-64- Precise Reproduction of Soft Tissue Structure around the Pontic Area Using Computer-Aided
Design and Manufacturing. Journal of Prosthodontics 28 (2019) 216–218- Effects of cementless fixation of implant prosthesis: A finite element study. The Journal ofAdvanced of Prosthodont 2019;11:341-349

Symposium II

Academic Societies2021-present
President
2020-present President
2020-present President elected2014-2020
Secretary
2018-2020
Vice President
2017-2019
President
2015-Present Vice President

Symposium I

1993-1995
1988-1990
1981-1987

MBA
Korea
PhD
MS
DMD

Joint Symposium

Education
2007-2010

Special Lectures

Brief CV

Symposium I
Monday, October 25th, 9:00-10:40
Video distribution and Live Session
SI-1
Honeycomb scaffolds: vertical bone augmentation andantibacterial effects

Koichiro Hayashi, Ph.D.
Department of Biomaterials, Faculty of Dental Science, Kyushu University

Alveolar ridge deficiencies prevent the long-term success of dental implants. Therefore, boneaugmentation is imperative when alveolar bone undergoes advanced resorption. Among various
bone defects, vertical bone defects are the most difficult to augment. Autogenous bone graft is still
the gold standard for vertical bone augmentation as it exhibits osteoconductive and osteoinductive
properties. However, the harvestable quantity of autogenous bone is limited and invasive surgery is
ineluctable. Furthermore, autologous bone is commonly associated with rapid resorption owing tomechanical pressure from soft tissues in the recipient sites. Guided bone regeneration (GBR) is
another common technique for bone augmentation. GBR provides a relatively predictable outcome
for bony defects. Nevertheless, complications attributed to the use of barrier membranes
occasionally occur. A synthetic scaffold with high osteoconductive and angiogenic abilities mayachieve barrier membrane-free GBR and resolve the current challenges in vertical boneaugmentation.
Scaffolds with uniaxial channels from the bottom face contacting the host bone to the top face,such as honeycomb (HC) scaffolds, are deemed to be favorable for vertical bone augmentation,compared to three-dimensional porous scaffolds. Furthermore, uniaxial channels in HC scaffolds
may restrict the entry of fibrous tissues. Herein, we developed HC scaffolds constructed byinterconnecting carbonate apatite microspheres. When the HC scaffolds with 230-, 460-, and630-μm-opening size channels were implanted on the rabbit calvarium, the amount of new boneand soft tissues in the HC scaffolds significantly increased and decreased, respectively, with thedecrease in the channel opening size. Thus, the HC scaffolds with 230-μm-opening size channels
prevented soft tissue invasion and enhanced bone ingrowth. The HC scaffolds with 300-μm struts
bore the compression for 12 weeks post-implantation and led to bone augmentation while being
replaced with new bone. These results provide significant insights into the scaffold characteristicsof the HC structure; it is inherently proper for vertical bone augmentation, and the multiscalearchitectural control of HC scaffolds may pioneer barrier membrane-free GBR while resisting
compression from soft tissues.
The prevention of surgical site infection (SSI) is required for favorable prognosis. To decrease therisk of SSI, we developed HC scaffolds with both osteoconductivity and antibacterial activity byreplacing a minute amount of carbonate apatite on the scaffold surface with silver phosphate. The
HC scaffolds exerted in vitro antibacterial effects for various bacteria without cytotoxicity.
Furthermore, when the HC scaffolds were implanted with methicillin-resistant staphylococcus
aureus (MRSA) into the defect of rabbit femur condyle. At 2 weeks post-implantation, no viable
MRSA was detected and new bone formed within the HC scaffolds. In contrast, in the groups
implanted with carbonate apatite HC scaffolds containing no silver phosphate, bacteriogenic
osteolysis and inflammation occurred. At 4 weeks post-implantation of the HC scaffolds composedof carbonate apatite and silver phosphate, new bone formation further progressed from 2 weeks
post-implantation and a portion of the HC scaffold was replaced by new bone.

─ 38 ─

Koichiro Hayashi
Department of Biomaterials, Faculty of Dental Science, Kyushu University

2015
2015
2016
2016

Rising Scientist Session

2020
2020

Outstanding Graduate Student Award, Nagoya University
Wakashachi Excellence Award, Aichi Prefecture
Best Teacher of The Year, The University of Tokushima
Best Presentation Award, The 25th Fall Meeting of the Ceramic Society of Japan
Young Investigator Award, The University of Tokushima
Oka Award, The University of Tokushima
Young Best Presentation Award, The 11st Annual Meeting of the Society ofNano Science and Technology
CerSJ Awards for Advancements in Ceramic Science and Technology, The
Ceramics Society of Japan
Best Presentation Award, The 28th Fall Meeting of the Ceramic Society of Japan
Academic Award, Tokai Chemical Industry Association
Best Presentation Award, The 32nd Annual Meeting of the Japan Society of Drug
Delivery System
Best-Research Award, Faculty of Dental Science, Kyushu University
Best Presentation Award, The 75th General Session of the Japanese Society for
Dental Materials and Devices

Symposium IIAward:
2007
2008
2012
2012
2013
2013
2013

Symposium I

Research & Professional Experience:
2009-2010
Research Fellowship for Young Scientists (DC2)
2010-2014
Assistant Professor, Institute of Health Biosciences, The University ofTokushima
2014-2017
Assistant Professor, Institute of Materials and Systems for Sustainability,
Nagoya University
2017-2018
Assistant Professor, Faculty of Dental Science, Kyushu University
2018Associate Professor, Faculty of Dental Science, Kyushu University

Joint Symposium

Education:
Undergraduate 2001-2005 Nagoya University, Nagoya, Japan
Graduate (master’s course) 2005-2007 Nagoya University, Nagoya, Japan
Graduate (doctoral course) 2007-2010 Nagoya University, Nagoya, Japan, Ph.D.

Special Lectures

Brief CV

Poster Presentation

─ 39 ─

Symposium I
Monday, October 25th, 9:00-10:40
Video distribution and Live Session
SI-2
Bone quality management effects of newly developed
dental implant design

Takashi Sawase, D.D.S., Ph.D.
Department of Applied Prosthodontics, Institute of Biomedical Sciences,
Nagasaki University

Bone quality and quantity are the crucial factors to achieve and maintain
Osseointegration. The early days during the end of 20 century, the bone quality inimplant dentistry was classified with the thickness of the cortical layer and the amountof the bone marrow. Whereas in 2000, the National Institutes of Health (NIH) has since
proposed a new clinical parameter of the “bone quality” from the series of studies on
osteoporosis. According to their definition, bone quality is “the sum total ofcharacteristics of the bone that influence on the fracture resistance.” The term of “bonequality” comprises bone architecture, bone turnover, bone mineralization and microdamage accumulation. Moreover, bone cells such as osteoblasts and osteocytes,
characteristics of collagen fibers, including type and alignment, and biological apatite(BAp) c-axis alignment are thought to be the determinant factors of bone quality.
Intriguingly, the c-axis of BAp is parallel to the extended collagen fibers associated withmechanical function, indicating that anisotropic changes in BAp c-axis/collagen fibers
regulate bone quality in response to mechanical loading.
The question arose what is the bone quality around the dental implant? Since, thedental implants are intrinsically subjected to dynamic loadings such as functional and/or
parafunctional forces via implant supported prostheses, we tried to clarify the bonequality around the implant by paying attention on the response to mechanical loading.
From the several animal experiments, the dynamic loading on the implant dramaticallychanges bone quality with increased osteocyte numbers/network, adapted preferentialalignment of BAp c-axis/collagen fibers and related cellular kinetics. Furthermore, thestudies also demonstrated that dynamic loading upregulates osseointegration, bonequantity (bone volume), and BMD around implants. Surprisingly, these anabolic effects
strongly depend on the implant thread design. The upward buttress thread design
which oriented in the same direction as the principal stress of the loading force could
enhance bone quality comparing to the downward buttress thread and triangle thread
designs.
In this presentation, I will introduce the newly developed implant design based on ourseries of research work.

─ 40 ─

Takashi Sawase, D.D.S., Ph.D.
Department of Applied Prosthodontics, Institute of Biomedical Sciences, Nagasaki University

Research & Professional Experience
1993-2000
Assistant Professor, Dept. of Fixed prosthodontics, Nagasaki Univ.
1996-1998
Visiting researcher at Dept of Biomaterials, Gothenburg University, Sweden
2000-2003
Assistant Professor, Nagasaki University Hospital
2003-2008
Associate Professor, Dept. of Fixed prosthodontics, Nagasaki Univ.
2009Professor, Dept. of Applied prosthodontics, Nagasaki Univ.

Joint Symposium

Education
Undergraduate:1983-1989 Nagasaki University School of Dentistry, Nagasaki, Japan, D.D.S
Graduate:1989-1993 Nagasaki University Graduate School of Dentistry, Nagasaki, Japan, Ph.D.

Special Lectures

Brief CV

Symposium I
Symposium II
Rising Scientist Session
Poster Presentation

─ 41 ─

Symposium I
Monday, October 25th, 9:00-10:40
Video distribution and Live Session
SI-3
Bioactive performance of octacalcium phosphate andits composite materials

Osamu Suzuki, Ph.D., FBSE
Division of Craniofacial Function Engineering, Tohoku University Graduate
School of Dentistry

Octacalcium phosphate (OCP, Ca8H2(PO 4)6·5H2O) has been advocated to be one ofprecursors in bone apatite crystal deposition during bone formation. Recently, one of
OCP composites with collagen was approved as a bone filling material for the use withdental implant in oral surgery field through a company-initiative clinical trial. We havefound first that OCP has a superior osteoconductivity in comparison withhydroxyapatite (HA, Ca10(PO4)6(OH)2) materials through an onlay graft experiment of
OCP granules onto mouse calvaria (Tohoku J Exp Med 164:37, 1991). Since then we havebeen developing OCP composite materials with polymers, such as reconstituted
collagen or other natural and synthetic polymers, to increase not only the handlingproperties but also bone regenerative properties. Concurrently, bone-related cellularresponses to OCP have also been analyzed to understand whether OCP itself canstimulate bone-related cellular activities in relation to its structural and chemical
properties.
Findings obtained were: 1) OCP-HA conversion accompanied by physicochemical
environmental changes around OCP is involved in enhancing bone regeneration(Biomaterials 27:2671, 2006); 2) OCP enhances osteoblastic differentiation of mousebone marrow stromal ST-2 cells dose-dependently (Tissue Eng Part A 14:965, 2008); 3)
OCP accumulates macrophages in the early stage of bone formation and enhances thecellular migration by the effect of ionic dissolution of OCP (Ca2+) (RSC Adv 6:57475,
2016); 4) OCP induces osteoclast formation in co-culture of osteoblasts and bonemarrow cells with expression of receptor activator of NF-kappaB ligand (RANKL) in
osteoblasts in the absence of external RANKL agent (Tissue Eng Part A 15:3991, 2009);
5) OCP enhances osteocyte differentiation from mouse bone marrow IDG-SW3 cells in
vitro and within newly formed bone matrices under contactless conditions (Acta
Biomater 69:362, 2018; 129:309, 2021); 6) OCP enhances bone regeneration more if
combined with polymers such as collagen (J Biomed Mater Res B Appl Biomater 79:210,
2006); 7) On of OCP composites with polymers OCP/Collagen is confirmed to effectively
be used in human or al b one defec t s with dent al implant (J T is sue Eng
11:2041731419896449, 2020) .
In this presentation, bioactive performance including bone regenerative capacity of OCP
composite materials brought about through the activation of bone-related cells, whichis induced by the materials function of OCP, will be explained and discussed.

─ 42 ─

Osamu Suzuki
Division of Craniofacial Function Engineering, Tohoku University Graduate School of DentistryAward:
2003
2003

2015
2020

Symposium II

2013

JGC Corp Award: The establishment in design basis of anti-fouling furnace tube
Award in the 23rd Engineering Advancement Association (ENAA) of Japan:
Engineering team for designing pharmaceutical facility with quantitativeevaluation containing potent compound. No.238.
Award in Reviewer for Grants-in-Aid for Scientific Research of Japanese Society
for Promotion of Science (JSPS)
Award of Japanese Society for Biomaterials: Elucidation of osteoconductivity
and establishment of biomaterial science of OCP bone substitute materials
Fellow, Biomaterials Sciences and Engineering (FBSE)

Symposium I

Research & Professional Experience:
1986-1994
Research Associate, Japan Fine Ceramics, Ltd., Research & Development
Division, Sendai, Japan.
1992-1994
Visiting Scientist, Forsyth Dental Center, Physical Chemistry Department,
Boston, MA, USA.
1994-1998
Associate Researcher, JGC Co., Research Center, Yokohama, Japan.
1999-2004
Senior Researcher, JGC Co., Research Center, Oarai, Japan.
2004-Present Professor, Division of Craniofacial Function Engineering, Tohoku University
Graduate School of Dentistry, Sendai, Japan.

Joint Symposium

Education
Graduate Student, 1984-1986 Yamagata University Graduate School of Engineering,
Yonezawa, Japan, M.Eng.
Ph.D., 1991 Tohoku University Graduate School of Medicine, Department of Orthopedic
Surgery, Sendai, Japan

Special Lectures

Brief CV

Rising Scientist Session
Poster Presentation

─ 43 ─

Symposium I
Monday, October 25th, 9:00-10:40
Video distribution and Live Session
SI-4
Periodontal regeneration by cytokine therapy- its present status and future perspective-

Shinya Murakami, D.D.S., Ph.D.
Department of Periodontology Osaka University Graduate School of Dentistry

Periodontitis is evoked by dental biofilms. Some success has been achieved in suppressing the
progress of periodontitis by mechanically removing the cause of the disease. However, noconventional periodontal and/or surgical treatments can regenerate periodontal tissue or its
functionality lost by periodontitis. On the other hand, several lines of evidence clearly
demonstrate that periodontal ligament (PDL) contributes to tooth nutrition, homeostasis, andthe repair/would healing of damaged periodontal tissue. Importantly, mesenchymal stem cells
and progenitor cells of hard-tissue forming cells, such as osteoblasts and cementoblasts, havebeen identified within the PDL. Thus, improving the biological potential of these cells andstimulating the regeneration of periodontal tissue are now recognized as clinically possible.
One of the most physiologically efficient methods to stimulate these cells is the use ofcytokines or growth/differentiation factors. Topical application of human recombinantcytokines to stimulate proliferation, migration and/or differentiation of those multipotent cells
may be an efficient way to accelerate the regeneration of periodontal tissue.
Basis Fibroblast Growth Factor (FGF-2) is known to stimulate the proliferation, migration anddifferentiation of a variety of cell types and to strongly induce angiogenesis. Animal studies
using beagle dogs and non-human primates demonstrated that topical application ofrecombinant FGF-2 induced statistically significant periodontal tissue regeneration with newly
formed cementum and alveolar bone. Further, in a series of human clinical trials, a significant
difference in % increase in alveolar bone height at 2- or 3-walled intrabony defects of thepatients was demonstrated by standardized radiographs between Placebo Group and 0.3%FGF-2 Group at 9 months after the treatment. Additionally, the efficacy of FGF-2 was notaffected by differences in age, sex, type of tooth, and vitality of dentin-pulp. Safety problems
were not identified in the studies. This reveals that topical application of 0.3%FGF-2 canstimulate regeneration of periodontal tissue of periodontitis patients.
In order to improve the therapeutic effect of the FGF-2 in a severe intraosseous defects ofperiodontal tissue or horizontal bone destruction, it is essencial to fully introduce the conceptof “tissue engineering”. Interestingly, some clinical studies suggest that combination therapy of
FGF-2 and bone graft material improves the efficacy of FGF-2. Further, it is demonstrated that
stimulation with FGF-2 promotes new bone formation around the dental implants andsubsequent osseointegration in a beagle dog model.
In this symposium, the present status of FGF-2 medicine in periodontal treatment will bepresented and the future perspective of the medicine in dental field will be discussed with theaudience.

─ 44 ─

Shinya Murakami
Department of Periodontology Osaka University Graduate School of Dentistry

2012
2013

Rising Scientist Session

2018
2019
2021

IADR/PRG: Anthony Rizzo Periodontal Research Award
American Academy of Periodontology(AAP):
R. Earl Robinson Periodontal Regeneration Award
IADR/AADR: William J. Gies Award
IADR: Distinguished Scientist Award
for Basic Research in Periodontal Disease.
IADR/PRG: Award in Regenerative Periodontal Medicine
AAP honorary membership Award
President Award of Japanese Association for Dental Science

Symposium IIAward:
1998
2009

Symposium I

Research & Professional Experience:
1988-1990
Visiting Fellow, National Cancer Institute,
National Institutes of Health, USA
1990-1992
Instructor, Department of Periodontology and Endodontology,
Osaka University Faculty of Dentistry
1992-2000
Assistant Professor, Department of Periodontology and Endodontology,
Osaka University Dental Hospital
2000-2002
Associate Professor, Department of Periodontology,
Osaka University Graduate School of Dentistry
2002Professor and Chair, Department of Periodontology,
Osaka University Graduate School of Dentistry
2011-2012
President, Japanese Division of the IADR (JADR)
2012-2013
President, Periodontal Research Group of the IADR
2016-2019
Director of Osaka University Dental Hospital
2019-2020
President, Japanese Society of Periodontology
2020Member of Science Council of Japan

Joint Symposium

Education
Undergraduate 1978-1984 Osaka University Faculty of Dentistry, Japan, D.D.S.
Graduate 1984-1988 Osaka University Graduate School of Dentistry, Japan, Ph.D.

Special Lectures

Brief CV

Poster Presentation

─ 45 ─

Symposium II
Monday, October 25th, 14:00-15:40
Video distribution and Live Session
SII-1
Single-cell RNA sequence from mouse incisor and molar
reveals dental epithelial cell-type specific genes

Satoshi Fukumoto
Section of Pediatric Dentistry, Kyushu University Faculty of Dental Science.
Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry

Identification of tooth-specific genes plays an important role in tooth development and tooth
regeneration researches. We have identified and functionally analyzed ameloblast-specificgenes by comprehensive gene screening such as cDNA microarrays. Through these screenings,
we succeeded in elucidating the important molecular mechanism in the differentiation processof ameloblasts. However, new molecular mechanisms in other epithelial cells that form teeth
germ except ameloblasts have not yet been elucidated. In this study, we analyzed using thesingle-cell RNA sequence (scRNA seq) method for the clarifying gene expression in all cell
populations that form tooth germs. In this screening, it became clear that ameloblasts, whichwere previously thought to be a single cell population, can be classified into two types: Dsppand Ambn-positive cells. The former was a cell having a function of secreting growth factors
and the like, and the latter was a cell secreting an extracellular matrix such as an enamel
matrices. We were also able to identify genes that are specifically expressed in inner and outer
enamel epithelium, stratum intermedium, and stellate reticulum. Subsequently, we identified
novel dental epithelial cell marker genes, namely Pttg1, Atf3, Cldn10, and Krt15. The results not
only provided a resource of transcriptome data in dental cells but also contributed to themolecular analyses of enamel formation.
We have identified genes expressed in tooth germs and conducted research to clarify themolecular function of mature ameloblasts, which had been a black box until now. Gpr115,
expressed in newly identified mature ameloblasts, was one of a family of molecules containing
G-protein coupled receptors. To investigate the in vivo function of Gpr115, knockout (Gpr115KO) mice were created and found to develop hypomineralized enamel, with a larger acidic area
because of the dysregulation of ion composition. Transcriptomic analysis also revealed that
deletion of Gpr115 disrupted pH homeostasis and ion transport processes in enamel formation.
In addition, in vitro analyses using the dental epithelial cell line cervical loop-derived dental
epithelial (CLDE) cell demonstrated that Gpr115 is indispensable for the expression of carbonic
anhydrase 6 (Car6), which has a critical role in enamel mineralization. Furthermore, an acidic
condition induced Car6 expression under the regulation of Gpr115 in CLDE cells. Thus, weconcluded that Gpr115 plays an important role in enamel mineralization via regulation of Car6
expression in ameloblasts. The present findings indicate a novel function of Gpr115 in
ectodermal organ development and clarify the molecular mechanism of enamel formation.
We have been advancing the functional analysis of the molecule by identifying genes that are
specifically expressed in tooth germs and mainly creating and analyzing gene-deficient mice.
From the results of our research, the whole picture of tooth development has been graduallyclarified, and it has greatly contributed to the understanding of the onset of human diseases
showing abnormalities in teeth including enamel hypoplasia.

─ 46 ─

Satoshi Fukumoto
Section of Pediatric Dentistry, Kyushu University Faculty of Dental Science.
Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry

Symposium I

Research & Professional experience
1994-1997 Instructor, Nagasaki University School of Dentistry1008-2000 Research fellow of the Japanese Society for the Promotion of Science
2000-2003 Instructor, Nagasaki University School of Dentistry2000-2002 Visiting Fellow, National Institute of Dental and Craniofacial Research (NIDCR),
National Institute of Health (NIH)
2003-2004 Assistant professor, Nagasaki University School of Dentistry2004-2007 Associate professor, Kyushu University, Faculty of Dental Science
2007- Professor, Tohoku University Graduate School of Dentistry2019- Professor, Kyushu University Faculty of Dental Science

Joint Symposium

Education
Undergraduate 1988-1994 Nagasaki University, Nagasaki, Japan, D.D.S.
Graduate 1997-2000 Nagasaki University Graduate School of Dentistry, Nagasaki, Japan, Ph.D.

Special Lectures

Brief CV

Symposium II
Rising Scientist Session
Poster Presentation

─ 47 ─

Symposium II
Monday, October 25th, 14:00-15:40
Video distribution and Live Session
SII-2
Recent Progress of Pulp Regenerative Therapy with
Dental Pulp Stem Cells for the Application to the
Periapical Disease in the Aged.
Koichiro Iohara
National Center for Geriatrics and Gerontology, Research Institute, Geroscience
Research Center, Regenerative Dental Medicine

With the continued increase in the longevity in our society the pulp regenerative
therapy may contribute to the functional survival and endurance of the tooth, leading
to the improvement of the health and welfare in the aged people by optimal
mastication with their functional teeth. Our previous clinical study and recent clinicaltreatments demonstrated the safety and efficacy of pulp regenerative therapy by the
autologous transplantation of dental pulp stem cells (DPSCs) with granulocyte-colony
stimulating factor (G-CSF) in pulpectomized teeth with irreversible pulpitis of the young
and middle-aged. On the other hand, the incidence of periapical disease is much higher
in people over 45 years old compared to patients with pulpitis in the aged. Thus, themain requirement for root canal treatment is shifting to periapical disease in the aged
due to pulpitis in the middle-aged.
Therefore, the first challenge is the complete disinfection prior to stem celltransplantation by irrigants and intracanal medication for the regenerative therapy for
periapical disease. We recently developed nanobubbles, which have the ability toremove the smear layer and enhance the delivery of medications to dentinal tubules,
thus demonstrating the potential utility of nanobubbles with antibiotics or antimicrobial
nanopolymers for the successful treatment of infected root canal and the resultant pulp
regeneration. The second challenge is to enhance pulp regeneration in aged teeth. We
have demonstrated that the age-dependent decline in regeneration was due to the
reduced migration, proliferation, and cell survival of resident stem cells. The potential
utility of trypsin pretreatment to enhance pulp regeneration and its underlying
mechanisms, the direct effects on resident stem cells and indirect effects on dentin andcementum have been demonstrated in aged dog teeth. The third critical challenge islimited availability of autologous stem cells. Therefore, development of off-the-shelfallogeneic DPSCs will be an advance. We initiated experiments in dogs and observed
optimal regeneration of pulp by the allogeneic transplantation of DPSCs both with thematched and mismatched dog leucocyte antigens (DLA).
In conclusion, it is possible to regenerate pulp tissue in the aged teeth in periapicaldisease by autologous and allogeneic transplantation of DPSCs after complete
disinfection and pretreatment of the root canal with trypsin.

─ 48 ─

Koichiro Iohara
National Center for Geriatrics and Gerontology, Research Institute, Geroscience ResearchCenter, Regenerative Dental Medicine

Award:
2002
2003
2004
2008
2013

Symposium II

2017
2021

Japanese Society of Conservative Dentistry Merit AwardInternational Symposium on Ultrasound Contrast Imaging Superb Poster Award
IADR Pulp Biology Travel Award
Japanese Society of Conservative Dentistry Encouragement AwardInternational Federation of Endodontic Association, The 9th World Endodontic
Congress, Best Presentation AwardThe 2017 Journal of Endodontics Best paper Awards
The 2020 Japanese Society of Conservative Dentistry Best paper award

Symposium I

Research & Professional Experience:
2005-2011
Researcher, Department of Oral Disease Research, National Institute forLongevity Sciences, National Center for Geriatrics and Gerontology
2011-2021
Chief, Department of Stem Cell Research Therapy, National Institute forLongevity Sciences, National Center for Geriatrics and Gerontology
2021Chief, Geroscience Research Center, Regenerative Dental Medicine, National
Institute for Longevity Sciences, National Center for Geriatrics and Gerontology

Joint Symposium

Education
Undergraduate 1995-2001 Kyushu University, Faculty of Dentistry, Fukuoka, Japan, D.D.S.
Graduate 2001-2005 Kyushu University Graduate School of Dental Science, Fukuoka, Japan,
Ph.D.

Special Lectures

Brief CV

Rising Scientist Session
Poster Presentation

─ 49 ─

Symposium II
Monday, October 25th, 14:00-15:40
Video distribution and Live Session
SII-3
Induced tissue-specific stem cell, a non-tumorigenic
intermediate cell induced from somatic or pluripotent
cell: their generation and possible use in theregenerative medicine of dental science
Issei Saitoh1, D.D.S., Ph. D, Masahiro Sato2, Ph. D, Emi Inada3,
D.D.S., Ph. D, Hirofumi Noguchi4, MD, Ph. D
1 Department of Pediatric Dentistry, Asahi University School of Dentistry
2 Department of Genome Medicine, National Center for Child Health and Development
3 Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental
Sciences
4 Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus

Induced tissue-specific stem cells (iTSCs) are partially reprogrammed cells, which arethought to be an intermediate state, like progenitors or stem cells. They originate duringtransition (de-differentiation) from differentiated somatic cells into pluripotent stem
cells [as exemplified by induced pluripotent stem cells (iPSCs) and embryonic stem cells(ESCs)], or during cellular differentiation of undifferentiated cells. iTSCs have a limited
capacity to differentiate and a morphology similar to that of somatic cell stem cells
present in tissues, but are distinct from that of iPSCs and ESCs. iTSCs can be generally
obtained 7 to 10 days after reprogramming of somatic cells with Yamanaka’s factors.
Once established, their fibroblast-like morphology remains unaltered. iTSCs can also be
obtained directly from iPSCs when they are maintained under the conditions allowing
cellular differentiation. Notably, for more effective induction into iTSCs, it requires
additional treatment such as conversion of iPSCs into naïve iPSCs, which are in more
undifferentiated state like mouse ESCs. iTSCs can proliferate continuously in vitro, butwhen transplanted into immunocompromised mice, they fail to generate solid tumors(teratomas), implying loss of tumorigenic potential. The low tendency of iTSCs to elicit
tumors is beneficial, especially considering applications for regenerative medicine inhumans. To date, several types of iTSCs have been identified. For examples, iTS-L, iTS-P
and iTS-D are obtained after partial reprogramming of hepatocytes, pancreatic cells,
and human deciduous tooth-derived dental pulp cells, respectively. Our presentation
will be focused on recent advances in the establishment of iTSCs and their possible
applications in regenerative medicine.

─ 50 ─

Issei Saitoh
Department of Pediatric Dentistry, Asahi University School of Dentistry

2019

Young Investigator Award of Japanese Society of Stomatognathic Function
Young Investigator Award of Japanese Society of Pediatric Dentistry
Academic Award “LION Award” of Japanese Society of Pediatric Dentistry
Machida Memoria Research Award of Japanese Society of Pediatric Dentistry
Excellent Paper Award of PEDIATRIC DENTAL JOURNAL
Machida Memoria Excellent Paper Award of J of Japanese Society of Pediatric
Dentistry
Poster Competition Award, The 57th Annual Meeting of Japanese Society ofPediatric Dentistry

Symposium IIAward:
2003
2008
2011
2015
2018
2018

Symposium I

Research & Professional Experience:
2005-2007
Assistant Professor, Department of Pediatric Dentistry, Kagoshima University
Graduate School of Medical and Dental Sciences
2007-2010
Lecturer, Department of Pediatric Dentistry, Kagoshima University Medical and
Dental Hospital
2008-2010
Visiting Researcher, Baylor College of Dentistry, USA
2010-2012
Associate Professor, Department of Pediatric Dentistry, Kagoshima University
Graduate School of Medical and Dental Sciences
2012-2021
Associate Professor, Division of Pediatric Dentistry, Graduate School of Medical
and Dental Sciences, Niigata University
2021Professor and Chairman, Department of Pediatric Dentistry, Asahi University
School of Dentistry

Joint Symposium

Education
Undergraduate 1993-1999 Kyushu University, Fukuoka, Japan, D.D.S.
Graduate 1999-2003 Kyushu University Graduate School of Dental Science, Fukuoka, Japan,
Ph.D.

Special Lectures

Brief CV

Rising Scientist Session
Poster Presentation

─ 51 ─

Symposium II
Monday, October 25th, 14:00-15:40
Video distribution and Live Session
SII-4
Exosomes secreted from TNF-α-preconditioned gingival
tissue-derived stem cells enhance M2 macrophage
polarization and inhibit periodontal bone loss
Takao Fukuda, D.D.S., Ph. D.
Section of Periodontology, Division of Oral Rehabilitation, Kyushu University
Hospital

Periodontitis is one of the most common osteolytic inflammatory diseases in humanthat adversely affects systemic disorders, such as diabetes. Accumulation of periodontal
bacteria-associated biofilm is thought to trigger periodontitis, but is not believed to be
sufficient to sustain the disease as the host immune response is critical for
inflammatory tissue breakdown and disease progression. Macrophages play animportant role in the immune response both during the initiation and resolution of
inflammation. Macrophages are broadly classified into two phenotypes, proinflammatory M1 and wound-healing M2 cells. Macrophages are involved in bonehomeostasis and the increased M1/M2 ratio leads to enhanced osteoclastogenesis. As
M2 macrophages contribute to the tissue-remodeling process, effective M2
macrophage induction would provide favorable environment for lower inflammation
and better regeneration.
We identified that gingiva-derived MSCs (GMSCs) have unique immunoregulatory
capacity and secret large amounts of exosomes. Considering the facts that therapeutic
effect of MSC largely depends on the paracrine efficiency of MSC and the advantages of
the use of GMSCs include easier isolation from small pieces of gingival tissue (~2×2mm2) and rapid cell proliferation, we hypothesized that GMSC-derived exosome-based
therapy could be suitable for clinical use. Moreover, recent studies indicated thatappropriate preconditioning of MSC with disease-related stimuli can optimize contentsof exosomes to efficiently support the repair of the tissues in particular diseases. In thiscontext, proteins or miRNA profiles in exosomes may be influenced by the pretreatment regimens. Therefore, optical molecular-based protocol for MSCpreconditioning needs to be investigated and established.
In this presentation, we report the therapeutic effects of TNF-α preconditioned-GMSCderived exosomes on periodontal disease and demonstrate underlying molecularmechanisms. Our study revealed that TNF-α-enhanced exosomal CD73 expression
leading to anti-inflammatory M2 macrophage polarization and exosomal miR-1260b was
important negative regulator of osteoclastogenesis. Accordingly, our findings may
provide a novel therapeutic strategy for patients with periodontitis and otherinflammatory osteoimmune disorders.

─ 52 ─

Takao Fukuda
Department of Periodontology, Division of Oral Rehabilitation, Kyushu University HospitalAward:
2014

Symposium I

Research & Professional Experience:
2000-2004 Department of Medical Biochemistry, Graduate School of Medical Sciences,
Kyushu University
2004-2014
Department of Periodontology, Division of Oral Rehabilitation, Kyushu
University Hospital
2014-2019
Assistant Professor, Department of Periodontology, Faculty of Dental Science,
Kyushu University
2016
Research fellow, Department of Anatomy and Cell Biology, Penn Dental
Medicine, University of Pennsylvania
2019Lecturer, Department of Periodontology, Division of Oral Rehabilitation, Kyushu
University Hospital

Joint Symposium

Education
Undergraduate 1994-2000 School of Dentistry, Kyushu University, Fukuoka, Japan, D.D.S.
Graduate 2000-2004 Graduate School of Dental Science, Kyushu University, Fukuoka, Japan,
Ph.D.

Special Lectures

Brief CV

Young Investigators Award, The Japanese Society of Conservative Dentistry
Symposium II
Rising Scientist Session
Poster Presentation

─ 53 ─RISING SCIENTIST SESSION

─ 55 ─

Rising Scientist Session
Monday, October 25th, 11:00-12:30
Video distribution and Live Session
RS-1
PPARγ-induced global H3K27 acetylation is required to maintain theabilities of extracellular matrix organization and osteo/cementogenesis
in periodontal ligament fibroblasts: the possible link between dietary
unsaturated fatty acids and periodontal tissue homeostasis
Shigeki Suzuki, D.D.S., Ph. D.
Department of Periodontology, Tohoku University Hospital

Dietotherapy is useful for prevention and treatment of lifestyle diseases such as diabetes mellitus,
arteriosclerosis, hyperlipidemia, and obesity. For dietary fat control, in addition to quantitative
restriction, qualitative selection is important. Generally, unlikely to saturated fatty acids, ingestion of aproper amount of unsaturated fatty acids, which include ω-3 fatty acid and ω-6 fatty acid, leads topositive systemic effects including thromboprophylaxis, hypotensive action, reducing serum LDL
cholesterol, and increasing serum HDL cholesterol. The unsaturated fatty acids bind to cell surface
receptors such as G protein-coupled receptors 120 (GPR120), GPR84, GPR41, and GPR43 and also areincorporated into cells and degraded by β-oxidation process. In inflamed periodontal tissue, ingestionof fresh oil, which is high in ω-3 fatty acids (docosahexaenoic acid and eicosapentaenoic acid), alteredeicosanoid production in immunoresponses and reduced chronic inflammation. In experimental mouse
periodontitis model, alveolar bone loss levels were inversely correlated with ω-3 fatty acid tissue level.
Moreover, ingestion of ω-3 fatty acids inhibits osteoclast differentiation and bone resorption. Thus, theunsaturated fatty acids inhibit the progression of periodontitis.
Fibroblasts in periodontal ligament tissue (PDLF: periodontal ligament fibroblasts) retain ability todifferentiate into osteo/cementogenic cells and aggressively synthesize and secrete collagen type 1 andthe molecules required for collagen fibrillogenesis in order to compensate for the fast turnover ofcollagen fibers in periodontal tissue, which is essential for maintaining connective attachments. Since
advanced periodontitis brings about hematogenous dissemination of activated immunological cells, oral
bacteria, and inflammatory cytokines, the maintenance of periodontal tissue homeostasis and theprevention of onset of periodontitis are the ultimate clinical goals in order not to exacerbate systemic
diseases. Therefore, the mechanisms by which PDLF utilize unsaturated fatty acids for periodontal tissue
homeostasis should be unveiled.
Peroxisome proliferator-activated receptor (PPARγ) is a nuclear receptor that plays a role in lipid
metabolism by directly regulating numerous metabolic genes. The dietary unsaturated fatty acids andtheir metabolites are main endogenous agonists of PPARγ. Therefore, PPARγ is the factor that responds
to external unsaturated fatty acids concentration. Indeed, PPARγ exerts anti-inflammatory effects inimmunological cells and osteoblastic cells as well as anti-osteoclastogenic effects in periodontal tissue.
PPARγ is the master regulator of bone marrow mesenchymal stem cells (BMMSC) for differentiation
towards adipogenic cells and inhibits differentiation towards an osteogenic cell lineage. However, theroles of PPARγ in PDLF have not been elucidated yet.
In this presentation, we demonstrate that PPARγ is a key epigenetic modifier in PDLF for the expressionof extracellular matrix (ECM)-related and osteo/cementogenic-related genes and also that the agonistic
effects of PPARγ enhance osteo/cementogenic abilities. PPARγ maintains an active chromatin status
marked with H3K27ac, particularly in the chromatin area in which RUNX2-binding sites were significantly
enriched. Consequently, the PPARγ-H3K27ac axis is a main machinery of PDLF to retain periodontal
tissue homeostasis and also the dietary unsaturated fatty acids probably keep the machinery inworking.

─ 56 ─

Shigeki Suzuki
Department of Periodontology, Tohoku University Hospital

Research and Professional Experience
2006
Department of Periodontology, Osaka University Graduate School of Dentistry2006-2009
National Institutes of Health, National Institutes of Dental and Craniofacial
Research, USA2009
Department of Biological Endodontics, Hiroshima University Graduate Schoolof Dentistry
2018
Department of Periodontology, Tohoku University Hospital

National Institutes of Health Visiting Program Award, USA
Encouragement Award, The Japanese Society of Conservative Dentistry
DENTSPLY Award, The Japanese Society of Conservative Dentistry

Symposium I

Award
2007
2010
2010

Joint Symposium

Education
Undergraduate 1996-2002 Osaka University, Osaka, Japan, D.D.S.
Graduate 2002-2006 Osaka University Graduate School of Dentistry, Osaka, Japan, Ph.D.

Special Lectures

Brief CV

Symposium II
Rising Scientist Session
Poster Presentation

─ 57 ─

Rising Scientist Session
Monday, October 25th, 11:00-12:30
Video distribution and Live Session
RS-2
Microbiome-targeted Probiotic Therapy Prevents
Hepatic Lipid-metabolism Abnormality following
Polymicrobial-Periodontal Infection via Improving Oraland Gut Dysbiosis
Ryutaro Kuraji, D.D.S., Ph. D.
Department of Life Science Dentistry, The Nippon Dental University, Tokyo, Japan
Department of Periodontology, The Nippon Dental University School of Life
Dentistry at Tokyo, Tokyo, Japan
Department of Orofacial Sciences, University of California San Francisco School of Dentistry

Nonalcoholic fatty liver disease (NAFLD), which is currently the most prevalent chronic liverdisease worldwide, is defined as cases showing the presence of hepatic steatosis but lacking
common causes of secondary hepatic fat accumulation, such as excessive alcohol consumption
or chronic viral hepatitis. The majority of NAFLD cases are simple steatosis with good prognosis,
but a subgroup of about 20%-30% of these patients can develop into more severe andprogressives forms of liver disease, namely nonalcoholic steatohepatitis (NASH). Moreover, aportion of NASH patients progress to cirrhosis and hepatocellular carcinoma, which are endstage liver diseases.
Recently, there has been a lively debate over the negative effects of periodontal disease onliver abnormalities, especially on NAFLD and NASH. Although the mechanism by which harmful
factors are transported from diseased periodontal tissue to the liver is unclear, two routes havebeen proposed based on the unique anatomical characteristics of the liver, namely ahematogenous diffusion via the systemic circulation and a gut-liver axis via the gastrointestinal
tract. Our research team previously reported that Porphyromonas gingivalis-derived
lipopolysaccharide diffused from periodontal tissues and accumulated markedly in the fatty
liver through the systemic circulation compared to the healthy liver controls.
In addition, gut microbiome dysbiosis induced by enteral translocation of periodontopathic
bacteria may be involved in NAFLD. One mechanism assumed to link the gut microbiome with
NAFLD is the disruption of the gut epithelial barrier, which may allow leakage of microbial
products and metabolites into the portal circulation. Namely, changes in lipopolysaccharide
and bacterial metabolites due to gut dysbiosis can induce intestinal inflammation and increase
permeability, thereby promoting hepatic exposure to these components, which can directly
cause NAFLD and liver fibrosis.
Diverse strategies for manipulating the gut microbiome in the management of NAFLD havebeen proposed, including the use of antibiotics and probiotics, which are defined as live
cultures of beneficial microorganisms for the human body. Antibiotics exert beneficial effects
on metabolic disorders by non-specifically suppressing the microbiome, but may be
accompanied by harmful side effects and potential emergence of antibiotic-resistant bacterial
strains. Therefore, recently, supplementation with probiotics and their products in thetreatment of NAFLD has been promoted due to their potential for enhanced safety for humans
and the environment.
However, little is known about the significance of probiotics for the management of NAFLD inpatients with periodontal disease. In this presentation, we report that oral and gut
microbiome-targeted probiotic therapy, especially with the probiotic bacteriocin nisin, which is
produced primarily by Lactococcus species, may be a useful approach in the management of
NAFLD associated with periodontal disease.

─ 58 ─Ryutaro Kuraji
Department of Life Science Dentistry, The Nippon Dental University, Tokyo, Japan
Department of Periodontology, The Nippon Dental University School of Life Dentistry atTokyo, Tokyo, Japan
Department of Orofacial Sciences, University of California San Francisco School of DentistryAward:
2017

Symposium II

2018
2018
2021

JSP/JACP Poster Session General/Basic Research Awards 1st Prize, American
Academy of Periodontology 102th Annual meeting in collaboration with theJapanese Society of Periodontology and Japanese Academy of Clinical
Periodontology.
Young Researcher`s Award, The Nippon Dental University.
Encouragement award 2018, The Japanese Society of Periodontology.
Young Scientist Encouragement Research Grant for Seeds Incubation, The
Japanese Society of Periodontology.

Symposium I

Research and Professional Experience:
2017-Present Assistant Professor, Department of Life Science Dentistry and Department of
Periodontology, The Nippon Dental University School of Life Dentistry at Tokyo
2019-2020
Visiting assistant professor, Division of Periodontology, Department of
Orofacial Sciences, University of California San Francisco School of Dentistry

Joint Symposium

Education and Training
2006-2012
D.D.S., The Nippon Dental University School of Life Dentistry at Tokyo
2012-2013
Resident for clinical training, Department of General Dentistry, Tokyo Medical
and Dental University
2013-2016
Ph.D., Department of Periodontology, The Nippon Dental University School ofLife Dentistry at Tokyo

Special Lectures

Brief CV

Rising Scientist Session
Poster Presentation

─ 59 ─

Rising Scientist Session
Monday, October 25th, 11:00-12:30
Video distribution and Live Session
RS-3
Infertility and Periodontitis

Kazuhiro Omori, D.D.S., Ph.D.
Department of Periodontics and Endodontics, Division of Dentistry,
Okayama University Hospital, Japan

In recent years, infertility has become one of social problems. The cause of infertility
may be male, female, or both, whereas it is reported that unexplained infertility, inwhich no obvious cause can be found by conventional infertility tests, accounts forabout 30% of all infertility cases. Therefore, there is a strong demand from society to
explore new approaches to unexplained infertility. Several risk factors for infertility
have been reported, such as age, obesity, stress, and smoking. Recently, periodontitis
has been reported to have an adverse effect on pregnancy, thus it may be a new risk
factor for infertility.
Periodontitis is an oral infection caused by the continuous infection of periodontal
pockets with periodontopathogenic bacteria such as Porphyromonas gingivalis (Pg),
resulting vicious circle of inflammation. It has been reported that infectedperiodontopathogenic bacteria and inflammatory cytokines produced by inflamedperiodontal tissues have a hematogenous effect on systemic diseases such as diabetes,
atherosclerosis, premature birth and low birth weight. Recently, it has been reported
that women with high salivary IgG antibody titers to Pg take longer to become pregnant
than women with low titers. Furthermore, it has been reported that reproductivefunction (sperm motility, etc.) is decreased in men with severe periodontitis. Thus, it issuggested that periodontitis may have some adverse effects on the ability to conceive
in both men and women. However, the mechanism regarding the effects ofperiodontitis on infertility is still unknown.
We are currently conducting a clinical study to investigate the level of Pg infection inpatients undergoing infertility treatment in cooperation with an obstetric clinic withreproductive center. In addition, we have investigated the effects of periodontal
infection/inflammation on uterine in mouse periodontitis model using immunologicalmethods. In this presentation, I would like to discuss the possibility of periodontitis as a
new risk factor for infertility.

─ 60 ─

Kazuhiro Omori
Department of Periodontics and Endodontics, Okayama University Hospital, Okayama, Japan

1995-2001
2001-2005

Okayama University Dental School, Okayama, Japan, D.D.S.
Graduate School of Medicine and Dentistry, Okayama
University, Okayama, Japan, Ph. D.

Research & Professional Experience:
2004-2008
Post-doctoral fellow, Department of Periodontology & Oral Biology, Graduate
School of Dental Medicine, Boston University, USA
2008-2009
MHLW Dentist, National Sanatorium of Oshimaseisho-en
2009-2014
Assistant Professor, Graduate School of Medicine, Dentistry and Pharmaceutical
Sciences, Okayama University
2014-current Senior Assistant Professor, Okayama University Hospital

The Japanese Society of Conservative Dentistry Encouragement Award
The 17th Kobayashi Magobei Memorial Foundation AwardThe 36th Ryobi Teien Memory Foundation AwardThe 20th Wesco Scientific Promotion Foundation Award
The Japanese Society of Conservative Dentistry Case Presentation Award

Symposium I

Awards:
2004
2010
2014
2017
2020

Joint Symposium

Education
Undergraduate
Graduate

Special Lectures

Brief CV

Symposium II
Rising Scientist Session
Poster Presentation

─ 61 ─

Rising Scientist Session
Monday, October 25th, 11:00-12:30
Video distribution and Live Session
RS-4
The role of chronic low-grade inflammation on energy
expenditure: association with CCL19/CCR7 axis

Misaki Iwashita, D.D.S., Ph.D.
Section of Periodontology, Division of Oral Rehabilitation, Faculty of Dental
Science, Kyushu University

The purpose of our research is to elucidate the molecular mechanisms on the systemic effects ofchronic low-grade inflammation like periodontitis originally evoked locally.
Previous intervention study in Japanese subjects with type 2 diabetes mellitus accompanied by severe
periodontitis demonstrated that patients with body mass index (BMI) around 25 kg/m2 showed slightly
increased high-sensitivity (hs)-CRP (over 500 ng/mL), and periodontal treatment significantly decreased
hs-CRP levels, followed by improved HbA1c. In contrast, subjects with lower hs-CRP (<500 ng/mL) werecharacterized by significantly lower BMI than those with hs-CRP 500 ng/mL or more. No significant
improvement in HbA1c was observed in subjects with lower hs-CRP following periodontal treatment. In
an animal study, we demonstrated that administration of low-concentration of lipopolysaccharide (LPS)
significantly upregulated serum inflammatory mediators as various cytokines and chemokines inhereditary and diet-induced obese mice compared with lean mice. These human and animal studies
suggest that local inflammation like periodontitis tends to be more amplified to the systemic level insubjects with overweight, suggesting that more matured adipose tissue play an important role insystemically amplifying local inflammation. Recent findings suggested that mature adipose tissue ischaracterized by massive infiltration of immune cells, primarily composed of monocyte/macrophagelineage. In this context, a comprehensive analysis of the gene expression in adipocytes co-cultured withmacrophages showed markedly increased gene expression of many CC and CXC chemokines. C-C motif
Chemokine ligand 19 (Ccl19) was one of the most highly expressed genes among all chemokine
examined in adipocytes. Highly elevated serum CCL19 level in obese individuals has also been reported
by others. We therefore hypothesized that CCL19 plays an important role in the pathophysiology of
adipose tissue-related metabolic disorder, and, thus, subsequent study focused on the role of CCL19
and its receptor, CC-chemokine receptor 7 (CCR7) (CCL19/CCR7 axis) on inflammation and energy
metabolism. Compared with wild-type (WT) mice, Ccr7-/- mice showed higher rectal temperature duringcold stimulation and significantly increased thermogenesis. Additionally, these mice were resistant tohigh-fat diet (HFD)-induced obesity, adipose and liver inflammation, fatty liver, and dyslipidemia.
Furthermore, the study using adipocyte-specific Ccl19 knock-in (KI) mice demonstrated that activated
CCL19/CCR7 pathway induced adipose tissue inflammation, and inhibited adenosine monophosphateactivated protein kinase α (AMPKα) activity in adipocytes via extracellular signal-regulated kinase 1/2(ERK1/2). The expression of uncoupling protein 1 (UCP1) was significantly reduced in brown adipose
tissue of Ccl19 KI mice compared with WT mice. These pathophysiological changes were most obvious inmice fed a 40% HFD than in mice fed a normal diet or a 60% HFD.
In summary, activation of CCL19/CCR7 pathway in adipose tissue induced higher inflammation andinhibited AMPKα through activating ERK1/2, resulting in impaired lipid metabolism and energy
regulation. In addition, a 40% HFD, which mimics western-style diet, more enhanced these pathologicalchanges.
Therefore, we suggest that local inflammation like periodontitis not only influence insulin sensitivity but
also affect energy regulation through adipose CCL19/CCR7 system.

─ 62 ─

Education
2006
2011

D.D.S., School of Dentistry, Okayama University, Okayama, Japan
Ph.D., Graduate School of Biomedical and Health Sciences, Hiroshima
University, Hiroshima, Japan

2012

President’s Poster Award, The American Diabetes Association's 70th Scientific
Sessions
Encouragement Award, The Japanese Society of Periodontology

Symposium I

Award
2010

Joint Symposium

Professional Experience
2006-2007
Dental resident, Okayama University Hospital
2011-2011
Clinical fellow, Hiroshima University Hospital
2012-2014
Assistant Professor, Graduate School of Biomedical and Health Sciences,
Hiroshima University
2014-2018
Assistant Professor, Kyushu University Hospital
2019Assistant Professor, Faculty of Dental Science, Kyushu University

Special Lectures

Brief CV

Symposium II
Rising Scientist Session
Poster Presentation

─ 63 ─

POSTER PRESENTATION

─ 65 ─

001:

Collagen-binding properties of Streptococcus mutans killed by amoxicillin
Y. SUEHIRO, S. MATAYOSHI, M. OTSUGU, R. NOMURA, K. NAKANO
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan

Objectives: Streptococcus mutans, a major pathogen of dental caries, is regarded as a causative agent of infective endocarditis(IE). For IE prevention, antibiotic therapy with amoxicillin is predominantly administered before an invasive dental procedure.
S. mutans cell surface proteins, such as 120-kDa collagen-binding protein (CBP) and 190-kDa protein antigen (PA), have effects
on its collagen-binding property and are considered to be involved in IE pathogenicity. Also, bacterial DNA encoding CBP isfrequently detected in heart valve specimens extirpated from IE patients, though live S. mutansorganisms are rarely isolated.
This study evaluated the collagen-binding properties of S. mutans strains after killing by amoxicillin.Methods: S. mutans strains
were divided into three groups, CBP+/PA- (n=10 strains), CBP+/PA+ (n=10), and CBP-/PA+ (n=10), then cultured under two
different conditions; living and killed by amoxicillin at 37°C for 18 hours. Next, each was added to a 96-well plate coated with
type I collagen and cultured at 37°C for three hours, then crystal violet staining was performed. The OD595 value of each strain
was determined, with the value of TW871 (CBP+/PA+) defined as 100%. Intergroup differences were compared using Student’s
t-test.Results: For the living S. mutans strains, the average collagen-binding rate for the CBP+/PA- group was 119.7±24.9%,
significantly higher than that for the CBP+/PA+ group (89.4±22.4%) (P<0.05), whereas the CBP-/PA+ group showed nearlyno collagen-binding ability. Among S. mutans strains killed by amoxicillin, the average collagen-binding rate for the CBP+/
PA- group was 96.4±26.7%, significantly higher than that for the CBP+/PA+ group (65.3%±10.3) (P<0.01), while the CBP-/PA+
group showed nearly no collagen-binding ability.Conclusions: CBP-positive S. mutans strains killed by amoxicillin demonstrated
collagen-binding properties, with that by the CBP+/PA- strains significantly greater as compared to the CBP+/PA+ strains. These
results suggest that CBP+/PA- strains killed by amoxicillin have a high level of pathogenicity for IE.

002:

S-PRG eluate inhibits acid and glucan synthesis by Streptococcus mutans
T. KITAMURA, S. MATAYOSHI, J. OHATA, Y. SUEHIRO, R. NOMURA, K. NAKANO
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan

Objectives: Surface pre-reacted glass-ionomer (S-PRG) filler, a bioactive material used in various dental materials, releases
six different ions, including fluoride (F-), sodium (Na+), borate (BO33-), aluminium (Al3+), silicate (SiO32-), and strontium(Sr2+). Streptococcus mutans, a major pathogen of dental caries, produces glucan and acids from sucrose, leading to dental
caries development. We investigated the inhibitory effects of S-PRG eluate towards S. mutans cariogenicity in the presence ofsucrose.Methods: S. mutans strain MT8148 was cultured in brain heart infusion broth containing 1% sucrose with or without
25% S-PRG eluate at 37°C for 18 hours. To determine the amounts of ions around S. mutans organisms, bacterial cells werecollected by centrifugation, resuspended in sterile distilled water, and subjected to sonication, then the amount of each ion wasmeasured. In addition, pH of the bacterial broths was determined with a pH meter and glucan concentration using a phenolsulfuric acid method. Statistical analyses were conducted using Student’s t-test, with P<0.05 considered to indicate significance.
Results: Among the six examined ions, amounts of BO33-, F-, Al3+, and Sr2+ around S. mutans were significantly higher in thepresence of S-PRG eluate as compared to its absence (P<0.001). In addition, the mean pH value of the bacterial solutions in thepresence of S-PRG eluate was 5.51±0.03, significantly higher as compared to without the eluate (pH 4.26±0.02) (P<0.001). On
the other hand, the mean glucan concentration in bacterial broth with S-PRG eluate was 34.2±4.3 μg/ml, significantly lower
than that without it (92.8±4.7 μg/ml) (P<0.001).Conclusions: Specific ions contained in S-PRG eluate were found around S.
mutans organisms. These ions have properties that inhibit cariogenicity of the bacterium in the presence of sucrose, such asacid production and glucan synthesis.

─ 66 ─

003:

Microbiological effects of silver diamine fluoride : an in situ study
1

Department of Restorative Dentistry and Endodontology, Graduate School of Dentistry, Faculty of Dentistry, Osaka University, 2Dental
Technology Institute, Osaka University, Osaka, Japan

Joint Symposium

Objectives: Supragingival biofilm is a main aetiologic factor of dental caries. Silver diamine fluoride (SDF) was experimentallyand clinically proven for the effectiveness in preventing and treating root caries, since it possesses the antimicrobial properties,
the efficacy in hardening tooth structure and eventually arresting caries. Our aim was to investigate the effect of SDF on
dental biofilm grown on demineralized dentin in in situ condition.Methods: Root dentin of bovine incisors were prepared into
rectangular slabs. Dentin slabs were divided into two groups; control and SDF groups, and were demineralized the surface by
20% citric acid. Unlike in control group, the dentin slabs in SDF group were treated with 38% SDF (Saforide™, Osaka, Japan) 4
min prior to the experiment. The dentin slabs were subsequently fit in our custom-made in situ intraoral devices. Seven healthy
volunteers were asked to wear the devices for 96 hours. Biofilms formed on the dentin slabs were harvested from the devices
every 24 hours. Viable cell counting, real-time PCR quantification, confocal microscopy and SEM observation were performed
to assess the microbiological changes.Results: CFU and PCR quantification results demonstrated lower number of viable andtotal bacterial cells respectively in SDF group than in control group. Confocal microscopy showed less thickness and volume
of biofilm in SDF group. Dead-to-live ratios in SDF biofilm were also significantly greater than in control group. Fewer biofilm
formation and less complex bacterial microcolonies of SDF group were observed from SEM images.Conclusions: In conclusion,under in situ condition of cultivating biofilm, SDF reduced the viability and biomass of biofilm including increased dead cells
were present. The anti-biofilm effect is an important mode in controlling and arresting root carious lesion.

Special Lectures

K. KLANLIANG1, Y. ASAHI1, H. MAEZONO1, T. SHIMAOKA1, N. KURIKI1, H. MACHI2, S. EBISU1, M. HAYASHI1

Symposium I

Investigation of Streptococcus mutans collagen-binding protein pathogenicity
D. MATSUOKA, S. NAKA, K. GOTO, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan

Poster Presentation

─ 67 ─

Rising Scientist Session

Objectives: IgA nephropathy (IgAN) is the most common form of chronic glomerulonephritis, accounting for half of all childcases. However, the mechanism for IgAN development remains unclear. We previously reported that Streptococcus mutans
with collagen binding protein (Cnm), which is associated with the collagen-binding ability of the surface protein of thisorganism, is frequently detected in the oral cavity of patients with IgAN. For the present study, recombinant Cnm (rCnm) wasconstructed and the pathogenicity of Cnm was investigated using a silkworm infection model.Methods: The cnm gene encodes
Cnm of S. mutans TW871, was amplified by PCR and ligated to a pGEX 6p-1 glutathione S-transferase fusion protein expression
vector. The constructed plasmid was transformed into E. coli BL21 (DE3), and transformed colonies were cultured and cells
harvested by centrifugation. Next, pelleted cells were suspended in PBS and ultrasonicated on ice. Supernatant was obtainedby centrifugation and purified using a glutathione SepharoseTM 4B column. Several different rCnm protein concentrations
were injected through the dorsal surface into hemolymph of the silkworms (n=10) using a 26-gauge needle. Following injection,
larvae were incubated at 37°C and survival was detemined every 12 hours for 204 hours.Results: Purified rCnm was confirmedby sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and CBB staining. Among the several different
concentrations of rCnm administered to the silkworms, a significant difference for survival rate after 204 hours was noted
between the group injected with PBS and the 50 μg rCnm injected group (P<0.05). Additionally, survival rates for the otherrCnm-injected groups were also lower than that of the PBS-injected group.Conclusions: These results indicate that the Cnm
protein of S. mutans is a pathogenic factor. Additional animal experiments will be necessary to clarify more effective dosage
concentrations and the pathogenicity of Cnm.

Symposium II

004:

005:

Analysis of specific Streptococcus mutans strains associated with non-alcoholic steatohepatitis
K. TABATA, S. NAKA, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan

Objectives: Non-alcoholic fatty liver disease (NAFLD) includes chronic hepatic diseases such as non-alcoholic fatty liver (NAFL)
and non-alcoholic steatohepatitis (NASH). In our previous studies, collagen-binding protein (Cnm)- and 190-kDa cell surface
protein antigen (PA)-positive Streptococcus mutans strains were shown to induce NASH aggravation. The present study
evaluated disease progression associated with Cnm- and PA-positive S. mutans using a NASH mouse model.Methods: Cnm- andPA-positive S. mutans KT3 strains isolated from a severe NASH patient, and Cnm- and PA-positive TW871 were used. C57BL/6J
mice (6-week-old males) were fed a high-fat diet for four weeks and then divided into the KT3 (n=16) and TW871 (n=9) groups,
which received administration via the jugular vein, and non-treated group (n=8). Twelve weeks after bacteria administration,
blood was collected before euthanasia and serum samples were obtained. Liver, visceral fat, and spleen weights were
measured. Furthermore, histological assessments of liver tissues were performed by hematoxylin-eosin (HE) and Masson'strichrome (MT) staining.Results: There were no significant differences for body weight or visceral fat weight among the threegroups, whereas liver weight for the KT3 group was significantly higher as compared to that of the non-treated group (P<0.05).
Serum levels of ALT, AST, TC, and LDL in the bacteria-treated groups were significantly higher than those in the non-treatedgroup (P<0.05). HE staining revealed marked lipid accumulation in liver tissues from all groups, with lobular inflammation
observed in both the KT3 and TW871 groups. In addition, MT staining showed marked fibrosis in liver tissues from the KT3 andTW871 groups.Conclusions: Cnm- and PA-positive S. mutans was found to be associated with development of NASH in mouse
liver tissues.

006:

Inhibitory effect of cyclodextran on caries in animal model
H. ASAUMI, M. MATSUMOTO-NAKANO
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan

Objectives: Cyclodextran, a cycloisomaltooligosaccharide, (CI), is known to function as a dextran analogue and considered
to be a possible sucrose substitute. CIs have a structure in which 7 to 12 glucoses are cyclically linked by α-1, 6 bonds. In aprevious report, a CI was shown to have inhibitory effects on glucosyltransferase B (GTFB) enzymes, which are produced by
the cariogenic bacterium Streptococcus mutans. In the present study, we examined the effect of CI to inhibit caries inductionin using model rats.Methods: Specific pathogen-free Sprague Dawley rats (male, 15 days old) were used. Antibiotic treatment
was performed at 15 and again at 16 days of age to facilitate establishment of S. mutans. S. mutans MT8148R strains were thendirectly administered into the oral cavity using a pipette once a day for five days, after which the rats were divided into fivegroups (n=15). They were fed a caries-inducing diet containing 56% sucrose (Diet 2000), or were given distilled water without CI
or with 0.625%, 1.25%, 2.5%, 5% CI. The rats were euthanized under anesthesia at the end of the experiment (73 days of age).
Bones were excised, and plaque and dental caries scores determined.Results: Results of both the diet and drinking experiments
showed that the amount of plaque adherence to tooth surfaces in all CI added groups was lower as compared to without
CI. Caries scores for the CI added groups were also lower.Conclusions: The present results suggest that CI reduces plaqueformation on tooth surfaces, which may also inhibit caries development. The cariostatic activity of this CI is considered to bemainly caused by its anti-GTF activity. This study was supported in part by the Fund for Scientific Promotion of Nissin Sugar Co.,
Ltd., Tokyo, Japan.

─ 68 ─

007:

Role of ABC transporter in biofilm formation by Streptococcus mutans
Department of Pediatric Dentistry, Okayama University Graduate School of Med icine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan

Joint Symposium

Objectives: Streptococcus mutans has been reported to be associated with dental caries induction and also forms biofilm. ATPbinding cassette (ABC) transporters exist in cell membranes and function for exporting and importing molecules. Zinc, known tobe essential for most forms of life, has structural, catalytic, and regulatory functions related to numerous biological processes.
In the present study, we performed functional analyses of the SMU1519 gene, predicted to be a zinc transporter, in regard to
biofilm formation by S. mutans.Methods: S. mutans MT8148 (serotype c) and an isogenic mutant strain with inactivation of
the SMU1519 gene (termed ΔSMU1519) were used. To estimate sensitivity to zinc (ZnSO4), growth rates of the tested strains
were evaluated. In addition, biofilm biomass formed by the tested strains was quantitatively measured at OD550 using crystal
violet staining, while biofilm vitality was assessed using an XTT assay. Real-time RT-PCR was performed with complementary
DNA samples using either 16S ribosomal RNA (rRNA) or specific primers for the SMU1519 gene.Results: The growth rate of
ΔSMU1519 was slightly lower than that of MT8148. On the other hand, in the presence of ZnSO4 growth was increased, andthe difference between MT8148 and ΔSMU1519 was eliminated. In addition, the biomass of biofilm formed by ΔSMU1519
was significantly lower than that of MT8148, while that of ΔSMU1519 was further lower as compared to that of MT8148 in thepresence of ZnSO4. Also, similar to the reduced biomass findings, ΔSMU1519 vitality was lower than that of MT8148. Finally,
the level of SMU1519 gene expression of MT8148 was significantly elevated in the presence of a low amount of ZnSO4 as
compared to its absence.Conclusions: These results suggest that zinc is a growth-limiting factor and SMU1519 contributes tozinc transport in S. mutans, which is related to biofilm formation by the organism.

Special Lectures

K. GOTO, M. MATSUMOTO-NAKANO

Symposium I

BCOR mediated regulation of ZFPM2 via BCL6 involved in hyperactive root formation of OFCD syndrome
K.M. SOE, T. OGAWA, K. MORIYAMA
Department of Maxillofacial Orthognathics, Tokyo Medical and Dental University (TMDU), Tokyo, Japan

Rising Scientist Session

Objectives: BCL6 interacting corepressor (BCOR) mutations cause oculofaciocardiodental (OFCD) syndrome, which is a rare
X-linked dominant condition, with permanent tooth radiculomegaly. Although BCOR-associated downstream molecules mightplay an important role in hyperactive root formation, its molecular regulation mechanism is far from being fully elucidated. The
objectives of this study were to determine these BCOR-associated molecules.Methods: Comparative expression profiling of
periodontal ligament (PDL) cells isolated from a normal and an OFCD patient were analyzed using microarray analysis. In vitro
testing included MTT assay, quantitative PCR, chromatin immunoprecipitation (ChIP) assay, and knockdown assay using OFCD
(mutant) PDL cells, human periodontal ligament fibroblasts (HPdLF), and COS7 cells.Results: The gene-specific transcriptional
factors group, wherein NFIB, DLX5, and ZFPM2 are the most upregulated genes, was significantly expressed in mutant PDL
cells. BCOR overexpression into HPdLF showed ZFPM2 downregulation and DLX5 upregulation, but NFIB expression did notchange. Focusing on ZFPM2, whose expression was downregulated after BCOR overexpression into HPdLF, ChIP assay using
HPdLF and COS7 cells showed that the wild-type BCOR was recruited in the BCL6 binding of the ZFPM2 promotor region after
immunoprecipitation, while the mutant BCOR, which is the same genotype to our patient, failed to recruit these promotor
regions. ZFPM2 knockdown expression in the mutant PDL cells also showed significantly reduced cellular proliferation andreduced ALP mRNA expression.Conclusions: Our findings suggested that BCOR mutation induced ZFPM2 regulation via BCL6,
possibly contributing to hyperactive root formation in OFCD syndrome.

Symposium II

008:

Poster Presentation

─ 69 ─

009:

In vitro degradation test of resorbable GBR membrane, Cytrans ElaShield
Y. SAKAGUCHI, K. YAMANAKA, F. FUSEJIMAGC Corporation, Tokyo, Japan

Objectives: Membranes are a key material to the GBR approach, being able to function as a barrier against fibrous tissue
and make the space for bone regeneration. We focused on the chemically synthesized and flexible biodegradable polymer
ε-caprolactone and L-lactic acid co-polymer (PLCL) and have developed a resorbable GBR membrane, Cytrans ElaShield. Acid
production during degradation become an issue of lactic acid-based polymer. Therefore, we evaluated a degradation behaviorof PLCL membrane and pH change during degradation.Methods: In vitro degradation test was performed in accordancewith the ISO15814:1999. φ13 mm specimens were soaked in phosphate buffered saline (PBS, pH 7.4) at 37 °C (n=4). Every 4
or 8 weeks, pH value of PBS solution was measured, and soak solution exchanged to fresh PBS if pH value is under 7. After
immersed, each specimen was rinsed and dried to check the mass loss. 50% mass loss period of PLCL was compared to that ofpoly (lactic-co-glycolic) acid (PLGA) membrane which degrades in 12-16 weeks in vivo.Results: As a result of in vitro degradation
test, membrane weight of each group was decreased in linearly from 8 weeks. Period of 50% mass loss was 28.8 weeks for PLCL
and 13.4 weeks for PLGA membrane. pH of PLCL changed from 7.4 to 6.9 for initial 12 weeks immersion. pH of PLGA changed
from 7.4 to 6.4 for initial 8 weeks.Conclusions: Degradation of both PLGA and PLCL membrane were slowly, and no rapid pHchange was observed. Furthermore, pH change of PLCL was slower than that of PLGA, and the amount of generated lactic acidcan be sufficiently metabolized by the buffer capacity in the body. Therefore, there will be no concern about inflammation
caused by a decrease in pH. These results indicated that Cytrans ElaShield is a clinically useful GBR membrane.

010:

The effects of MTA exposed to NaOCl on odontoblastic differentiation of a human periodontal ligament
stem cell line
K. YAMASHITA1, A. TOMOKIYO2, T. ONO2, K. IPPOSHI1, A. ALHASAN1, A. TSUCHIYA3, A. HAMANO2,4, H. SUGII1, S.
YOSHIDA2, T. ITOYAMA2, H. MAEDA1,2
1
Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, Fukuoka, Japan, 2Department ofEndodontology, Kyushu University Hospital, Fukuoka, Japan, 3Department of Biomaterials, Faculty of Dental Science, Kyushu University,
Fukuoka, Japan, 4OBT Research Center Faculty of Dental Science, Kyushu University, Fukuoka, Japan

Objectives: White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a darkbrown color when in contact with sodium hypochlorite solution (NaOCl). However, the detailed structures and hard tissueforming ability of NaOCl-exposed WMTA have been unclear. Therefore, this study aimed to investigate the effects of NaOClon the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells(PDLSCs).Methods: Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in
distilled water (DW) or 5% NaOCl for 24 hours. Their surface structures were analyzed by X-ray diffraction, scanning electron
microscopy, and energy dispersive X-ray spectrometry. The amounts of Ca2+ released from the discs were also investigated.
Moreover, they were cultured with a clonal human PDLSC line (line 1-17 cells).Results: The main crystal structures of WMTA
discs in contact with NaOCl were identical to the structures of discs immersed in DW. Globular aggregates with polygonal
and needle-like crystals were found on WMTA discs exposed to DW and NaOCl, which included Ca, Si, Bi, C and O. However
many amorphous structures were also identified on NaOCl-exposed discs. These structures consisted of Na and Cl, but did notinclude Ca. NaOCl immersion also reduced Ca2+ release from whole WMTA discs. Line 1-17 cells cultured with WMTA discs
immersed in DW formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However,cells incubated with NaOCl-treated discs generated a small number of nodules and showed low expression levels of osteoblastrelated genes.Conclusions: These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous
structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate
osteoblastic differentiation of PDLSCs.

─ 70 ─

011:

K. TANAKA1,2, A. TSUCHIYA2, Y. OGINO1, Y. AYUKAWA1, K. ISHIKAWA2
1

Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan,
Department of Biomaterials, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

2

1

Division of Biomaterials, Department of Oral Functions, Kyushu Dental University, Fukuoka, Japan, 2Division of Operative Dentistry,
College of Dental Medicine, Rangsit University, Pathum-Thani, Thailand

Poster Presentation

─ 71 ─

Rising Scientist Session

Objectives: This study aimed to develop novel filler-dispersed resin composite for fabrication of a permanent fixed-partial
restoration by additive manufacturing. Mechanical properties (MP), physicochemical properties (PP), cell viability (CV), andconversion degree (CD) were evaluated.Methods: Resin-mixture precursors were prepared with variations of filler size andpercentage (microfiller: 20%; 40%; 60%; 70% and nanofiller: 10%; 20%; 30%; 40%). Then the specimens were fabricated by
SLA 3D-printer. Flexural strength (FS), flexural modulus (FM), Vickers hardness (VH) were evaluated both immediately andafter 2months of water-immersion. Water-sorption (WS) and water-solubility (WL) were evaluated after 2months of waterimmersion. The optimal precursor composition would receive additional CV (by CCK-8 assay at day1, day3, day5, day10) andCD (FTIR) evaluations. Data were analyzed by one- or two-way ANOVA followed by Tukey’s test (p=0.05).Results: For both filler
types, higher filler-content groups demonstrated better MP and PP. However, higher filler-content precursors also had higher
viscosity leading to difficult manipulation and defects. Comparing between same filler-content groups, microfiller groups
exhibited higher VH, WL, lower FM, WS (p<0.05) than nanofiller groups but FS was not different. Water-immersion decreased
FS and VH, but not FM for both filler types. 60% microfiller group (FS = 168.4±10.1MPa, FM = 6.3±0.4GPa and VH = 62.6±0.9,
WS = 30.7±1.13μgmm-3, WL = 3.9±0.8μgmm-3) was chosen for CV and CD tests. CV results of experimental material revealed
same level of biocompatibility as commercial CAD-CAM block (76.2±18.5% and 89.3±5.2% respectively). CD was 49.9±3.7%.
Conclusions: The precursor with moderately high microfiller content, in this experiment 60%, seemed to be the most suitablecomposition for 3D-printer. Moreover, its mechanical and physicochemical properties complied with ISO 10477:2018 and
JDMAS 245: 2020. The experimental material is promising and worth further research and development.

Symposium II

Development of Novel Filler-Dispersed Resin Composite for 3D-Printed Permanent Crown
P. KARNTIANG1,2, H. IKEDA1, Y. NAGAMATSU1, H. SHIMIZU1

Symposium I

012:

Joint Symposium

Objectives: Interconnected porous structure may be a key for use of carbonate apatite (CO3Ap) block for massive bonereconstruction. In this research, feasibility to fabricate three-dimensional interconnected porous CO3Ap block was studied
based on hydrate expansion of calcium oxide (CaO) granules, followed by carbonation and phosphatization (Figure 1). Also, its
potential for bone reconstruction was histologically investigated using rabbits.Methods: CaO granules were hydrated under
100% humidity in a closed vessel, and exposed to CO2 at 25°C. Then, samples were immersed in Na2HPO4 solution at 80°C.
XRD and FT–IR were used for compositional analysis, whereas SEM and μ–CT were used for structural analysis. Potential of
the porous CO3Ap as artificial bone was evaluated by reconstructing the rabbit femur bone defects. Dense CO3Ap blocks wereused as controls.Results: After exposing to the humidity, CaO granules expanded and formed three-dimensional interconnected
porous structure. XRD and FT–IR results along with SEM and μ–CT analysis confirmed successful compositional transformation
to CO3Ap maintaining its structure (Figure 2). Histological analysis 4 weeks after surgery revealed that new bone wasformed interior of the porous CO3Ap block. Additionally, remodeling of the porous CO3Ap was confirmed by the presence ofosteoblasts, osteoclasts and osteocytes. 8 weeks after surgery, red blood cells and vascular endothelial cells were confirmed
inside the pores, indicating a Haversian system-like structure formation. By contrast, new bone was observed only on thesurface of dense CO3Ap blocks even after 8 weeks (Figure 3).Conclusions: Three-dimensional interconnected porous CO3Ap
block was successfully fabricated using hydrate expansion of CaO granules, followed by carbonation and phosphotization. Theinterconnected porous structure was useful to allow penetration of the bone-related cells interior of the block. We concluded,
therefore, that the three-dimensional interconnected porous CO3Ap block has a good potential value for treatment of massivebone defects.

Special Lectures

Bone reconstruction using three-dimensional interconnected porous CO3Ap block fabricated based on
hydrate expansion of CaO granules.

013:

Morphological observation of microvascular changes and bone formation after Alveolar boneregeneration.
M. MATSUO, M. TO
Department of Clinical Oral Anatomy, Kanagawa Dental University, Yokosuka, Japan

Objectives: Various kinds of bone regeneration materials have been tried to regenerate the alveolar bone. In this study,
synthetic and xenograft materials were used for morphological analysis. This research concerns observation of the relationshipof the vascular network and alveolar bone using a stereoscopic and scanning electron microscope (SEM).Methods: All animal
experiments were conducted according to the protocol agreed by our Institutional Animal Ethics Committee. Six beagle dogs
were used. Both mandibular premolars were extracted. Right side of tooth sockets was filled with Carbonated apatite granule(CO3Ap: Cytrans granule, GC, Japan) with L-lactide-ε caprolactone copolymer membrane (Cytrans elashield, GC, Japan) as asynthetic graft group. Left side was filled with bovine porous bone graft material granule (Bio-Oss, Geistlich, Switzerland) withporcine collagen membrane (Bio-Gide , Geistlich, Switzerland) as xenograft group. The vascular synthetic resin was injectedfrom inferior alveolar arteries after postoperative 14, 30 and 90days. Microvascular resin specimens were examined bystereoscopic microscope and SEM.Results: Postoperative fourteen days in both groups, granule filled the extraction sockets
tightly. Newly formed blood vessels from the pre-existing bone marrow penetrated the spaces between the granules in thesocket. Thirty days after surgery, the bone addition had progressed where the blood vessels were buried in the newly formed
bone. Bone formation in the central region of sockets is more advanced in the synthetic graft group than xenograft graft group.
Nineteen days after , Extraction socket was regenerated the dense compact bone. Thick blood vessels formed a network aroundthe granules. The height of alveolar margin was maintained in both groups.Conclusions: The results of this research indicatethat both materials are beneficial for bone regeneration. In the future we have to decide whether animal-derived materials or
chemically synthesized materials are more beneficial to patients.

014:

Tuning macrophage polarization by titanium nanosurfaces through nanotopographic cues to prevent periimplantitis
N. KARTIKASARI, M. YAMADA, H. EGUSADivision of Molecular and Regenerative Prosthodontics, Tohoku University graduate school of Dentistry, Sendai, Japan

Objectives: Definitive prevention of peri-implantitis remains unestablished. M1 macrophages play a key role in innate immunity
against inflammatory osteolysis around biomaterials. Macrophage polarization is controlled by environmental stimuli and hence
a titanium nanosurface might tune macrophage polarization and function. The purpose of this study was to determine whether
titanium nanosurfaces with dense nanospikes prevent peri-implantitis by tuning macrophage polarization toward inhibiting
osteoclast differentiation of osteoclast precursors.Methods: Nanoroughened titanium surfaces created by alkaline-etchingtreatment were evaluated for the surface properties. Mouse macrophage-like cell line (J774A.1) were cultured on machined,
micro-roughened, or nanoroughened titanium surfaces and analyzed for the relationship between macrophage polarizationand the surface properties. Mouse osteoclast precursor cell line (RAW264.7) were co-cultured with osteoclast differentiation
factors and macrophage culture supernatants from each titanium surface and evaluated for osteoclast differentiation. Microroughened or nanoroughened titanium mini-implants osseointegrated with the rat’s maxilla were ligated to induce periimplantitis, followed by histologic evaluations of inflammatory osteolysis.Results: Nanoroughened surfaces had anisotropicallypatterned dense nanospikes together with superhydrophilicity, as well as hydroxyl groups. J774A.1 cells on nanoroughened
surfaces exhibited M1-like circular shapes and highly expressed M1, but not M2, markers, even under the conditions thateliminated the surface’s superhydrophilicity and hydroxyl groups. The other titanium surfaces hardly evoked any polarization
markers in macrophages. Macrophage culture supernatants on nanoroughened surfaces inhibited osteoclast differentiationof RAW264.7 cells in contrast to differentiation into large TRAP-positive cells in the supernatants from the other surfaces.
Nanoroughened titanium implants significantly inhibited peri-implant bone resorption and osteoclastogenesis compared tothose around the micro-roughened titanium implants.Conclusions: Titanium nanosurfaces with dense nanospikes can prevent
peri-implantitis by tuning macrophage polarization through nanotopographic cues toward inhibiting osteoclast differentiationof osteoclast precursors.

─ 72 ─

015:

Effect of repeated use of end mill on the internal fitness of complete crown.
Department of Fixed Prosthodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo,
Japan

Joint Symposium

Objectives: A 5-axis milling machine has achieved the good internal fitness of the complete crown. However, it is unclear how
many times the cutting bar can be used. This study aims to evaluate the effect of repeated use of the diamond-coated end
mill on the internal fitness of crowns fabricated using a CAD/CAM system.Methods: The abutment tooth was prepared for
a complete crown using the lower 1st premolar with 1.0 mm rounded shoulder and 1.5-2.0 mm occlusal reduction. The 130
crowns were milled from a composite resin with 50 μm internal spacing using a 5-axis milling machine. The internal fitness ofcrowns was measured by the silicone replica technique. The silicone in the cement space was sectioned, and the thickness wasmeasured using a micron depth and height measuring instrument. The surface morphology of the end mill was observed usinga scanning electron microscope (SEM). The surface texture and roughness of crowns were evaluated by a laser microscope.
Results: A marginal and axial wall area discrepancies increased after around 80 repeated uses of an end mill. Up to 80 times,
the discrepancies of a marginal area were under 100 μm, which has been reported as the value for the clinical tolerance. SEM
images of the end mill showed that the diamond coatings peeled off after 10 repeated uses, and most of the coatings at theedge of the end mill detached from the base material after 80 repeated uses. Although the chipping of the coated surface wasobserved after using 10 times, the discrepancies were not affected. Similarly, the surface roughness rapidly increased after
80 repeated uses.Conclusions: This study indicated that the repeated use of end mill should be within 80 times to get goodinternal fitness of crown.

Special Lectures

K. NOZAKI, K. KOMINE, W. YANAKA, M. MATSUMURA, S. OMNIA, C. SHIN, M. MATSUMURA, H. MIURA

Symposium I

A novel bioabsorbable polysaccharide derivative for minimally invasive bone graft harvesting
K. NAKANISHI1, T. HASEGAWA2, H. HAYASHI3, K. NAGAMOTO4, H. OGUMA1, T. AKASAKA1, Y. YOSHIDA1
1

Department of Biomaterials and Bioengineering, Hokkaido University, Sapporo, Japan, 2Department of Developmental Biology of Hard
Tissue, Hokkaido University, Sapporo, Japan, 3Clinical Research and Medical Innovation Center Research and Development Division,
Hokkaido University Hospital, Sapporo, Japan, 4Department of Oral Diagnosis and Medicine, Hokkaido University, Sapporo, Japan

Poster Presentation

─ 73 ─

Rising Scientist Session

Objectives: The cancellous bone harvested from the ilium is commonly utilized for bone grafting of the cleft lip and palate;nevertheless, it leads to heavy burdens on children with clefts. The bone regeneration ability of implanted cancellous bone
is high, so that the development of alternative materials remains difficult and challenging. Here we developed a novel
bioabsorbable polysaccharide derivative termed “phosphopullulan” and investigated its safety and function.Methods: To
survey the biological safety of phosphopullulan, the following tests were conducted: The skin sensitization test in guinea pigs,
pyrogen test in rabbits, intracutaneous reactivity test in rabbits, acute systemic toxicity test in mice, implantation test in rabbit
bone, subchronic toxicity study by implantation in the rat medullary cavity, bacterial reverse mutation test, In vitro mammalianchromosome aberration test, In vitro cytotoxicity test, and endotoxin test. Furthermore, the granular or powdered artificial
bone mixed with or without phosphopullulan was implanted in the rabbit bone to test the inflammatory potential.Results: It
was established that phosphopullulan has high biological safety. Phosphopullulan was phagocytosed by macrophages withbasophilic and foamy cytoplasm, indicating CD204+ M2 type, which is involved in wound healing and tissue repair. In theimplantation site, no other inflammatory cells were discovered. The artificial bone implantation test showed that the powdered
artificial bone triggers inflammation but could also hinder the inflammatory reaction when mixed with phosphopullulan.
Granular artificial bone takes years to be resorbed and replaced in the bone, hampering tooth eruption and increasing infection
risk. Furthermore, phosphopullulan provided artificial bone with formability and operability. Phosphopullulan can solvethese existing challenges regarding artificial bones.Conclusions: Phosphopullulan is not only extremely safe but also has ideal
functions as a material for implantation in the body, expecting to become a key material for minimally invasive bone graft
harvesting.

Symposium II

016:

017:

Bond Strengths of 4-META/MMA-TBB Resin Cement to Root Canal Dentin
T. OZAKI, S. OTAKE, W. KOMADA, K. NOZAKI, Y. YOKOSUKA, H. MIURA
Fixed Prosthodontics, Tokyo Medical and Dental University, Tokyo, Japan

Objectives: The purpose of this study was to evaluate an adhesion promoting effect of 4-META/MMA-TBB by the pretreatment
method of root canal dentin in resin build-up restoration.Methods: Six freshly extracted single root human teeth were usedin this experiment. After pulpal tissue were removed by endodontic files, root canals were prepared to be one-third of thediameter of the root section width and two-third of the vertical root length. After root canal preparation, each root was
longitudinally hemisected and pulpal dentin walls were polished flat. One of the roots sectioned into halves was used as theimmersion group, the other was the control group. Dentin surface treatment of the control group was treated with Green
Activator (Sun Medical) in 10 sec. The immersion group was treated with Green Activator in 10 sec and Teeth Primer (Sun
Medical) in 30 sec and immersed in a large amount of Super-Bond Activated Liquid (Monomer : Catalyst V = 4:1) (Sun Medical)
for 1min. Commercially available resin composite block was bonded to treated dentin with 4-META/MMA-TBB (Super-Bond)
after sandblasting (Al2O3, 70 μm, 0.1 MPa) and treating with PZ Primer (Sun Medical). After storage in a 100% humidity
incubator at 37°C for 1 hour, all specimens were stored in water at 37°C for 24 hours in darkness and were shaped hourglass
configuration with a cross-sectional area of approximately 1mm2 for micro-TBS testing. The specimens were loaded in thetesting device (AUTOGRAPH AGS-H, Shimadzu) at a CHS of 1.0 mm/min. Data were analyzed by paired sample t-test (p<0.05).
Results: Micro-TBS values between the immersion group (60±7.94 MPa) and the control group (38±17.6 MPa) was statisticallysignificant difference(t=2.955, p<0.05).Conclusions: The immersion in the activated liquid was effective method to promote a
root canal dentin adhesive.

018:

Ceria-stabilized zirconia substrate directly bonds to bone-like hydroxyapatite crystals at nano-scale
M.M. SAITO, Y. YAMAKOSHI
Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, Yokohama, Japan

Objectives: The osseointegration ability of ceria-stabilized tetragonal zirconia polycrystals/aluminum oxide (Ce-TZP/Al2O3),
a promising dental implant material, has been unclear at microscopic level despite its recent clinical use. Our objective is
to characterize the crystal structures of the cell-induced precipitates and investigated the bonding interface between the
precipitates and ceria-stabilized zirconia at nanoscale.Methods: C2C12 cells were cultured on a Ce-TZP/Al2O3 substrate. The
differentiation potency was investigated by qPCR analysis for differentiation marker genes in C2C12 cells. The nodule formationand mineralization capacities of the cells were assessed with Alizarin Red S staining. Proteins in cell-induced precipitates wereextracted and characterized by SDS-PAGE. Cell-induced precipitates were characterized by X-ray diffraction, scanning electron
microscopy, transmission electron microscopy (TEM) and scanning-TEM energy-dispersive X-ray spectroscopy.Results: The
inorganic component of precipitates consisted of hydroxyapatite single phase that exhibited a characteristic morphology, i.e.,
flexible nanofibers less than 10 nm wide with nanometer-thick plates filling the spaces between nanofibers. No metastablephases such as amorphous calcium phosphate and octacalcium phosphate were found in the precipitates. The precipitates had
two bone proteins, osteocalcin and osteopontin, as organic substances. High-speed and less-destructive elemental analysis
revealed that the hydroxyapatite contained alkaline metal cations (Na, Mg, and K) as minor elements and that its average Ca/
P atomic % ratio was ~1.40, similar to that of bone apatite. This hydroxyapatite directly bonded to the zirconia nanocrystals
at lattice fringe scale, indicating nanoscale osseointegration. Crystal structure models showed that the hydroxyapatite face
could grow epitaxially on the zirconia face.Conclusions: These findings provide crucial information about the osseointegrationof ceria-stabilized zirconia, which facilitates the further study using this material both in fundamental science and application
fields, and contribute to improving the success rate of dental implant treatment. This study was supported by a JSPS KAKENHI
Grant-in-Aid for Early-Career Scientists (15K21457 and 19K19078).

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019:

The Relationship Among Child Dental Caries, Microbiome and Parental Awareness.
1

R&D, Sunstar Inc., Hyogo, Japan, 2Sunstar Foundation, Osaka, Japan

Joint Symposium

Objectives: Parental awareness is the key to preventing child dental caries. The purpose of this study was to assess thedifferences in salivary microbiome between mother and child, the child microbiome differences due to their caries history
and the relationship between child salivary microbiome/caries history and parental awareness.Methods: Saliva, examinations
and questionnaire were collected from Children and/or Mothers (64). The V3-V4 region of 16S rRNA gene was amplified andsequenced on Illumina MiSeq platform. Sequencing analysis was performed against the Human Oral Microbiome Database(HOMD). Children were classified by caries history into three groups: Non-caries (42), Former caries experience (12) and Current
caries (10) or two groups: Non-caries (42) and Caries (22). There were six questions about child oral care that his/her motherusually took care of in the questionnaire and those were answered by mothers.Results: The mother and child microbiome
was significantly more similar than the non-mother and child microbiome. Streptococcus mutans and Scardovia wiggsiae thathave been suggested to be associated with caries, were significantly more abundant in Caries group than Non-caries group.
Comparing the three caries groups, only S. wiggsiae was significantly less abundant in Non-caries group than Former caries
experience group and Current caries group. The all current caries teeth were mild and followed closely without treatment,
while former caries teeth were severe and required treatment. Mothers of children with current caries performed more oral
care habits than mothers of children with former caries.Conclusions: The children with caries had more caries-related bacteria
than without caries (Non-caries). Parental oral care may be important as well as child oral care, since the mother and childmicrobiome was more similar. This study suggests that enhancing parental awareness of oral care and continuing appropriate
oral care habits make it possible to reduce the risk of caries occurrence and progression.

Special Lectures

Y. KURIYAMA1, E. MIYAMOTO2, M. OHARA2, N. YASUDA1, Y. SHIMIZU1, S. OKAMURA1

Symposium I

Involvement of Human Salivary Protein-derived Peptides-specific SIgA In Oral P. gingivalis Colonization.
K. KOYANAGI1, K. KATAOKA1,2, S. YANAGISAWA2, T. MIYAKE1
1

Department of Preventive and Community Dentistry, Osaka Dental University, Osaka, Japan, 2Department of Oral Health Science andSocial Welfare, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan

Poster Presentation

─ 75 ─

Rising Scientist Session

Objectives: We have previously showed that fimbriae on Porphyromonas gingivalis (Pg), one of putative periodontopathogenic
bacteria, specifically bound to a peptide domain (stat23, prp21) shared by statherin or acidic proline-rich protein 1 (PRP1),
human salivary proteins. In this study, we investigated whether double DNA adjuvant (DA) consisting of DNA plasmid expressing
Flt3 ligand (pFL) and CpG oligodeoxynucleotide (CpG ODN) would induce stat23- and prp21-specific secretory-IgA (SIgA)
antibodies (Abs) in saliva of mice administered nasally stat23 and prp21 plus DA, and the induced SIgA would inhibit Pg-binding
to human whole saliva-coated hydroxyapatite beads (wsHAPs).Methods: Female 8-weeks-old, C57BL/6 (IgA+/+) or IgA-deficient(IgA-/-; background C57BL/6) mice were nasally immunized with respectively 50 mg of stat23 and prp21 as antigens (Ags)plus 50 mg of pFL and 10 mg of CpG ODN as DA four times at weekly intervals. Seven days after the last immunization, saliva
samples were collected, and examined stat23- and prp21-specific IgA antibodies (Abs) responses by ELISA and ELISPOT assay.
In addition, SIgA-enriched saliva samples deleted both IgG and IgM by using affinity columns were tested in the Pg -binding
inhibition assay to wsHAPs.Results: Significant induction of salivary SIgA Abs to stat23 and prp21 was seen in IgA+/+ mice.
Furthermore, the SIgA-enriched saliva samples induced in mice administered nasally the mixture of stat23 and prp21 with DA
showed significant Pg-binding inhibition to wsHAPs. Of interest, saliva samples in IgA knock-out mice given nasally Ags plus
DA displayed no inhibitory effects on Pg-binding to wsHAPs.Conclusions: These findings showed that human salivary proteinderived peptides-specific SIgA Abs induced by nasal administration with stat23 and prp21 plus DA have a potential inhibitory
effect in Pg colonization to wsHAPs in vitro, and suggested that SIgA induced by the nasal vaccination might be an effective tool
preventing Pg colonization on tooth surface by cohering fimbriae-binding domains on salivary proteins.

Symposium II

020:

021:

The Composition and Structure of Oral Microbiome: 16S rRNA Gene Analysis on Saliva, Plaque, Infected
Root Canals obtained from a Patient
K. YAMAKI1, T. TAMAHARA2, J. WASHIO3, T. SATO4
1

Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan, 2Department ofCommunity Medical Supports, Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan, 3Division of Oral Ecology
and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai, Japan, 4Division of Clinical Chemistry, Niigata University
Graduate School of Health Sciences, Niigata, Japan

Objectives: Over 700 species of bacteria have been identified in the human oral cavity, and many of those bacteria are sitespecific. As one can harbor approximately 100-200 of them, there is substantial diversity among individuals, and efforts todesignate key pathogens in apical periodontitis had been failed. Because there are few reports on microbial comparison of oral
specimens taken from the same person, this study aimed to profile the microbiome of saliva, plaque and infected root-canaldentin obtained from one patient by 16S rRNA gene analysis.Methods: The subject was a 58-year-old male patient visiting
Tohoku University Hospital for dental treatment. Clinical and radiographic examination revealed apical periodontitis of teeth
42 and 31. Saliva, supragingival plaque, canal-wall-dentin samples were collected under informed consent. After sampling,
conventional root canal treatment was conducted on both teeth. Samples were kept at -80°C and thawed upon analysis.
DNA extraction was performed using UCP Pathogen Mini Kit, and 16S rRNA amplicons of V3-V4 region were amplified andsequenced on Illumina MiSeq platform. Data were analyzed using QIIME2 with SILVA.Results: The microbial composition of
those 4 samples were different not only at the genus-species level, but also at much higher phylum level. Root canal samples
were rich in Firmicutes and scarce in Actinobacteriota and Proteobacteria. Tooth 42 harbored 29 phylotypes and Dialister,Anaeroglobus, Veillonella were the main components of its Firmicutes. Although 17 phylotypes were detected in tooth 31, it
was dominated by Pseudoramibacter (41.5%) and Fusobacterium (50.7%). As for Actinobacteriota, Rothia and Actinomyces
were predominant in saliva and plaque, respectively. Proteobacteria found in saliva and plaque were mainly Neisseria.
Conclusions: These findings suggested that the microbial profile varies considerably within an individual’s oral cavity. As teeth
with similar symptoms had quite different intra-canal microbiomes, the concept of ‘microbial community as pathogen’ in the
etiology of apical periodontitis was underscored.

022:

Bone apatite-clusters in intramembranous and endochondral ossification show distinct morphologies
E. HARA, M. OKADA, T. MATSUMOTO
Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama,Japan

Objectives: The objective of this study was to perform a comparative analysis of the structure and composition of bone apatite
clusters formed in intramembranous (IO) and endochondral ossification (EO).Methods: For analysis of IO, calvaria were isolated
from ICR mice at embryonic days (E) 14.5 to 15.5. For analysis of EO, femur epiphysis and diaphysis were isolated from newborn
mice at post-natal days (P) 6 to 7 and embryos at E15.5, respectively. Bone apatite crystals were also analyzed during bone
repair in a 1-mm femur defect model in 6-week-old ICR female mice. The morphology of bone apatite clusters was analyzed
by scanning electron microscopy (SEM) after selective removal of organic matter in the bones with NaClO. The NaClO-treatedbones were washed and dehydrated before SEM observation. The composition of bone apatites were analyzed by inductivity
coupled plasma-optical emission spectroscopy (ICP) and energy dispersive X-ray spectroscopy (EDS). Biomimetic mineralization
assay was performed to investigate the role of pH in apatite cluster growth. The mineralization products were analyzed by X-ray
diffraction. Image analysis was performed with ImageJ.Results: SEM observation and image analysis revealed that bone apatite
clusters in EO (epiphysis) were > 10 times larger than those in IO (calvarium). No significant changes in ion composition weredetected by ICP or EDS. Similar findings were observed in femur diaphysis, where cortical (IO) and trabecular (EO) bone can beobserved at the same site. During femur repair, the minerals formed in the callus through EO were also similar to those formed
in epiphysis and diaphysis, but were gradually resorbed until day 14 of healing. The minerals formed through IO inside the bone
defect were similar to those observed in calvaria. In vitro mineralization assay suggested that a slow P supply into a Ca-richalkaline microenvironment could facilitate the formation of large apatite clusters.Conclusions: The results revealed the distinct
morphologies, without significant difference in ion composition, of bone apatite clusters in IO and EO.

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023:

A Materials Science-based Re-evaluation of Initial Tooth Mineralization
1

Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama,
Japan, 2Department of Orthodontics, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,
Okayama, Japan, 3Advanced Research Center for Oral and Craniofacial Sciences, Okayama University, Dental School, Okayama, Japan

Joint Symposium

Objectives: The objective of this study was to re-evaluate the initial stages of mouse molar formation from a materials science
viewpoint.Methods: For identification of the initial site and time of molar mineralization, pregnant mice were injected withcalcein (20 mg per kg) at embryonic day (E) 17.5, and calcein-positive mineralized areas in the first molar were observed undera fluorescence stereoscopic microscope. The molars were isolated at E18.5, postnatal day (P) 0 and P1. Ultrastructural analysisof the initial mineralization process was performed with scanning (SEM) and scanning-transmission electron microscopy (STEM)
using resin-embedded P0, P1 and P3 first molars. Elemental mapping and electron diffraction were performed for qualitative
analysis of the initial minerals in dentin and enamel.Results: At E18.5, calcein staining was not observed, and the morphologyof the crown was not clear. At P0, the morphology of the crown was well-defined, and the initial calcification indicated bycalcein staining was observed at the tip of the distobuccal cusp. At P1, the calcein-stained calcified area was observed along
the morphology of the crown surface layer. SEM / STEM observations revealed that calcification begins in the dentin andoccurs in the following steps: 1. collagen deposition, 2. formation of amorphous calcium phosphate (ACP), and 3. crystallization
into apatite. Subsequently, enamel formation was observed, and similar to dentin, involved the initial formation of ACP andtransformation into apatite, as analyzed by electron diffraction.Conclusions: Based on a systematic and spatio-temporal analysis
of initial tooth formation stages, this study identified the very initial minerals in P0 mouse molars at the distobuccal cusp. ACP
was found to be a precursor of apatite in both dentin and enamel.

Special Lectures

R. ANADA1, E. HARA1, N. NAGAOKA3, M. OKADA1, H. KAMIOKA2, T. MATSUMOTO1

Symposium I

Effects of TGF-β isoforms on ameloblasts
Y. MIYAKAWA1, Y. YAMAKOSHI2, Y. ASADA1
1

Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, Yokohama, Japan, 2Department of Biochemistry andMolecular Biology, Tsurumi University School of Dental Medicine, Yokohama, Japan

Rising Scientist Session

Objectives: Transforming growth factor beta (TGF-β) is one of the physiologically active substances produced by various cells inthe body, and three types of isoforms (TGF-β1, β2, β3) are known in mammals. In general, TGF-β isoforms transmit signals into
cells via a common receptor, and it has not been clarified whether there are different signal transductions between isoforms.
Our objective: is to compare the cell morphology and the results of gene expression analysis in order to clarify whether there
is a difference between isoforms in the signal transduction of TGF-β1, β2 and β3.Methods: Mouse enamel epithelial cell line
(mHAT9d) was seeded on a 6-well plate. Upon confluent, TGF-β1, β2 and β3 was individually added and cultured for 8 days.
After culture, cell morphology was observed by Rhodamine-Phalloidin and DAPI staining. In addition, comprehensive gene
expression analysis (next-generation sequencing) was performed using mHAT9d cultured under the same conditions for 10
days.Results: In cell morphology, paving stone-like cells were observed in the control, but cells stimulated with TGF-β1 and β3
lost their morphology and possessed the cell process. Many cells with paving stone-like morphology were observed in TGF-β2
as well as control, and it was found that there were few cells with the cell process compared to TGF-β1 and β3. From theresults of next-generation sequencing, there were categories in which the expression level did not increase when TGF-β2 wasadded compared to the control. In addition, the gene expression levels of kallikrein 4 and carbonic anhydrase 2 were found to
be increased in the group to which TGF-β1 and β3 was added as compared with control and TGF-β2.Conclusions: TGF-β reacts
differently between isoforms to ameloblasts.

Symposium II

024:

Poster Presentation

─ 77 ─

025:

Decreased expression of Neprilysin induced by P. gingivalis-LPS in mouse hippocampus
T. MORIKAWA1, O. UEHARA2, D. PAUDEL1, K. YOSHIDA1, J. SATO1, Y. ABIKO1
1Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences
University of Hokkaido, Hokkaido, Japan, 2Division of Disease Control and Molecular Epidemiology, Department of Oral Growth andDevelopment, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan

Objectives: Alzheimer's disease (AD) is the most frequent neurodegenerative disorder in ageing populations in the world.
Although periodontal diseases may be a risk factor of AD, the mechanism is still unknown. The deposition of the amyloid-β
peptide (Aβ) in the brain parenchyma is a crucial step in AD. Aβ is usually eliminated from the brains by clearance mechanism.
The overproduction of Aβ or a lack of the clearance mechanism induce increased deposition of the Aβ in the brain. Neprilysin(NEP) is a an enzyme that in humans is encoded by the metallo-endopeptidase gene. NEP is thought to be involved in theclearance mechanism. In the present study, we examined the effects of systemic administration of Lipopolysaccharide derived
from P.gingivalis (PG-LPS) on NEP expression in the hippocampus of adult and senescence-accelerated mice.Methods: Eightto ten week-old C57BL/6J mice and SAM-P/8 mice were administrated LPS derived from P. gingivalis intraperitoneally once
every 3 days for 3 months. The normal serine was administrated as a negative control. We extracted the hippocampus frommice. NEP expression levels are measured by quantitative RT-PCR (qRT-PCR), immunofluorescence staining and western blot
analysis. The data were statistically analyzed using Mann-Whitney’s U test.Results: Eight to ten week-old C57BL/6J mice and
SAM-P/8 mice were administrated LPS derived from P. gingivalis intraperitoneally once every 3 days for 3 months. The normal
serine was administrated as a negative control. We used the hippocampus extracted from mice as samples. NEP expression
levels are evaluated by quantitative RT-PCR (qRT-PCR), immunofluorescence staining and western blot using anti-NEP antibody(Proteintech Group, Inc.). The data were statistically analyzed using Mann-Whitney’s U test.Conclusions: These results indicatethat decreased expression of NEP may be involved in AD associated with P. gingivalis.

026:

Tetrahydrobiopterin pathway: A therapeutic target for neuropathic pain model
S. RAMAN, A. WASKITHO, R. RAJU, M. OSHIMA, Y. MATSUKA
Department of stomatognathic function and occlusal reconstruction, Tokushima University, Tokushima, Japan

Objectives: Tetrahydrobiopterin (BH4) is an essential cofactor involved in the production of several neurotransmitters. Previous
research reports suggested upregulation of BH4 in chronic pain. This study aimed to evaluate the effect of a novel drug,
Tranilast in blocking the BH4 pathway in chronic neuropathic pain model.Methods: Four weeks old male Sprague-Dawley rats
were used. Trigeminal neuropathic pain was induced by infraorbital nerve constriction (IONC). Tranilast (50/75/100/200 mg/kg) or Carbamazepine (30 mg/kg) or saline were injected intraperitoneally (1ml) for evaluating the response of pain postintervention. von Frey's behavior test was done for measuring the mechanical sensitivity of the whisker pad area. The rotarod
performance test was done for evaluation of motor coordination (n = 6 in each group). Trigeminal ganglia were excised andtested for BH4 RT2 Profiler PCR Array (n = 6).Results: von Frey test showed significant changes in the ipsilateral side of the nerve
injury model (p < 0.05). Tranilast (75, 100 & 200 mg/kg) and Carbamazepine responded well with significant changes in pain
tolerance (p < 0.05). The rotarod performance test showed, no significant changes in the Tranilast group whereas a reduced
retention time on the rod for Carbamazepine treated group which signifies reduced motor coordination. The BH4 array analysis
showed significant changes in Spr and Akr genes. These genes code for one of the key enzymes, sepiapterin reductase involvedin the production of BH4.Conclusions: Current medications for neuropathic pain show relative efficacy and cause disturbing
side effects. Tetrahydrobiopterin (BH4) mechanism would be a new avenue for neuropathic pain intervention. Our results aresuggestive of the use of Tranilast in the treatment of trigeminal neuropathic pain.

─ 78 ─

027:

Fermented dairy food intake reduces risk of tooth loss in a Japanese community
1
Section of Preventive and Public Health Dentistry, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan, 2OBT Research Center, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

Joint Symposium

Objectives: Consumption of a variety type of dairy foods has been reported to be associated with oral health status. However,the longitudinal association between fermented dairy food intake and oral health has not been well elucidated. This study aims
to explore this association in the population-based study.Methods: In 2012, 1,966 residents in Hisayama town, Japan, aged
40-79 years, received dental examinations. Among them, 1,469 participants were followed up in 2017 for incidence of tooth
loss representing ultimate oral health, which was defined as two and more teeth lost over a 5-year period. A semi-quantitative
food frequency questionnaire grouped into quartiles was used to evaluate the intake of fermented dairy foods, such as yogurt
and lactic acid beverages. The salivary microbiota composition was evaluated.Results: The cumulative incidence of tooth loss
over 5 years was 17.7%. The Poisson regression model showed a negative association between higher fermented dairy foods
intake and incidence of tooth loss (p for trend = 0.020) after adjusting for age, sex, toothbrushing frequency, regular dental
visit, smoking, body mass index, diabetes, number of teeth at baseline, job, and number of decayed and filled teeth at baseline.
The mediation analysis confirmed that periodontal condition partly mediated the effect of fermented dairy foods intake on
tooth loss, while dental caries experience did not. Additionally, we revealed the association between high intake of fermented
dairy foods with low percentage of the salivary microbiota patterns which was connected to poor oral health.Conclusions:
These findings suggest that a higher intake of fermented dairy foods is associated with a lower risk of tooth loss resulting fromperiodontal disease via a probable change in oral microbiota composition.

Special Lectures

J. MA1, M. FURUTA1, T. TAKESHITA1,2, S. KAGEYAMA1, M. ASAKAWA1, S. SUMA1, Y. YAMASHITA1

Symposium I

Two opposite effects of desmoglein 3 expression on the growth of oral squamous cell carcinoma between
anchorage-dependent and -independent conditions
J. INADA1, M. MINABE1, Y. AKIYAMA1, K. HIGA2, T. TACHIKAWA3, S. TAKAHASHI4, M. KOUNO4, T. NOMURA1
1

Department of Oral Oncology, Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan, 2Cornea Center Eye bank, Tokyo
Dental College Ichikawa General Hospital, Chiba, Japan, 3Division of Molecular Diagnosis and Cancer Prevention, Saitama Cancer
Center, Saitama, Japan, 4Department of Dermatology, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan

Poster Presentation

─ 79 ─

Rising Scientist Session

Objectives: Desmoglein 3 (Dsg3) is one of the desmosomal cadherins that mediate epithelial intercellular adhesion. Dsg3 isoverexpressed in oral squamous cell carcinoma (OSCC), and considered as a candidate biomarker for lymph node metastasis.
Previously we have demonstrated that OSCC cells from metastatic lymph nodes showed higher Dsg3 expression, comparedto those from the primary tumor of the same patients. Here, we investigated the effect of Dsg3 expression on the growth of
OSCC cells between anchorage-dependent (AD) and -independent (AID) conditions.Methods: Cell lines established from theprimary tumor (P cells) and metastatic lymph nodes (LY cells) of three OSCC patients (No.7, 17, 58) and two commercial OSCC
cell lines (HSC3, SAS) were cultured under AD and AID conditions. Cell proliferation ability was quantified using by WST-1 cellproliferation assay. Cell migration ability was evaluated by wound healing assay. Dsg3 expression was examined by real-time
qPCR.Results: Under AD conditions, P cells (Dsg3-low) showed higher proliferation and migration rates than LY cells (Dsg3high) in the same patients. In contrast, under AID conditions, the ability of cell proliferation and multicellular aggregation of
Dsg3-high cells were higher than the Dsg3-low cells of the same patients. After transferred into AID conditions, all the celllines except for Dsg3-negative 7P showed increased Dsg3 expression by up to 50-fold, compared with AD conditions. Dsg3
knockdown under AD conditions increased the cell proliferation ability of all the cell lines except for 7P cells.Conclusions: Ourfindings suggested that Dsg3 may have inverse effect on the growth of OSCC cells under AID conditions. Inverse effect of Dsg3
expression on the AID cell growth could be associated with lymph node metastasis of OSCC.

Symposium II

028:

029:

Associations between dental caries and Helicobacter pylori infection: A cross-sectional pilot study
K. IWAI, T. AZUMA, T. YONENAGA, T. TOMOFUJI
Department of Community Oral Health, School of Dentistry, Asahi University, Gifu, Japan

Objectives: Helicobacter pylori (H. pylori) is widely known as a cause of gastric disorders. Presence of H. pylori in dental pulp
has been reported. Dental caries may influence the presence or absence of H. pylori infection by serving as a source of H.
pylori. In this cross-sectional pilot study, we examined whether H. pylori infection were associated with dental caries.Methods:
A total of 210 patients were recruited. Information about family history, medical history, and lifestyle were collected using
a questionnaire. In addition, oral conditions including decayed, missing, and filled (DMF) teeth, and community periodontal
index (CPI) score were measured. Urine antibody test was utilized to detect H. pylori infection.Results: The prevalence of H.
pylori infection was 14%. The number of decayed teeth were higher in patients with H. pylori infection than in those without
H. pylori infection (p< 0.05). The logistic analysis showed that presence of H. pylori infection was positively associated withnumber of decayed teeth (OR, 3.717; 95% CI, 2.292 to 6.026) after adjusting for age, gender, family history of gastric cancer,
heart disease, stress, CPI score, number of decayed teeth, and number of missing teeth. Furthermore, the prevalence rate of
H. pylori infection increased according to number of decayed teeth (p< 0.05). This study was indicated that H. pylori infection
rate in subjects with two or more decayed teeth was 55% (23/42).Conclusions: The results indicate that H. pylori infection wereassociated with number of decayed teeth. We would like to express our gratitude to the Department of Dental Medicine, Kyoto
Prefectural University of Medicine, Graduate School of Medical Science (Director: Professor Narisato Kanamura) and otherrelated parties for their cooperation in this study.

030:

Application and Investigation of Total Adenylate(ATP, ADP, AMP) Hygiene Tests for Oral Health Monitoring
B. ALTANKHISHIG, M. YASUHIRO, M. KHATUN, T. SAITODivision of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Science University of
Hokkaido, Hokkaido, Japan

Objectives: Prevention of oral inflectional diseases requires good oral hygiene, and a test method that can objectively measure
it is needed. Recently, a test method using adenosine triphosphate (ATP) as a marker has been developed. On the other hand,
new instruments have been developed that can detect ATP, adenosine diphosphate (ADP), and adenosine monophosphate(AMP). This study compared ATP analysis methods with total adenosine nucleotide (ATP, ADP, and AMP) analyzers.Methods:
CariScreen (CS, Oral Biotechnologies) as an ATP detection instrument and LuciPac A3 Sanitation System (A3, Kikkoman
Biochemifa) as a total adenosine nucleotide (ATP, ADP, AMP) analyzer were prepared. 1) Examination of detection sensitivity
A standard sample (100 μL) was diluted ten times with the ENLITEN® ATP Assay System (Promega Corporation), and thefluorescence level (RLU) was measured. (2) Measurement of the bacterial suspensions S. mutans (ATCC 25175) was cultured
anaerobically in BHI medium for 24 h, and the sample was prepared at a density of 1 × 109 cells/mL. From 1 ml of the preparedsample, a bacteria-removed sample was prepared by 0.2 μm filtering, and a bacteria-killed sample was prepared by heating
at 100C for 10 min. Each instrument measured the fluorescence level (RLU) of 100 μL of each sample. The results were
statistically analyzed using t-tests (*p<.05).Results: A3 showed a higher relative light unit (RLU) value than CS, indicating that
A3 could detect the presence of bacteria with high sensitivity, whereas CS could not detect the RLU value in the supernatantsolution. In addition, in the heat-sterilized sample, the RLU of CS was lower than that of viable bacteria, whereas A3 was higher.
Conclusions: These results indicate that A3 could detect the presence of bacteria, even in the absence of bacteria.

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031:

Chemical direct convesion of human fibroblasts into functional osteoblastic cells
1
Department of Dental medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan, 2Department of Immunology, Kyoto
Prefectural University of Medicine, Kyoto, Japan

Joint Symposium

Objectives: Large jawbone defects after removal of jawbone cyst or tumor, and tooth loss induced by alveolar bone resorption
associated with advanced periodontitis decrease patient’s quality of life (QOL). The osteoblasts play central roles in boneformation and remodeling by producing calcified bone matrix. A procedure to generate functional osteoblasts from humansomatic cells will be effective regenerative therapy for bone disorders. Thus, we had established procedures to directly convert
human fibroblast into functional osteoblasts by transducing some transcription factor genes. However, genetic manipulations
cause safety concerns and are not desirable in most of clinical applications. From this perspective, we tried to establish
a procedure that realizes chemical molecules-driven conversion of human fibroblasts into osteoblasts.Methods: Normal
human fibroblasts were cultured with various types of small molecules in addition to conventional osteogenic medium. The
resultant cells were tested in vitro for expressions of OB-specific genes, the production of calcified bone matrix, and global
gene expression pattern. Moreover, to assess in vivo efficacy, the resultant cells were transplanted into immunodeficientmice.Results: Osteoblast-like phenotypes including high alkaline phosphatase (ALP) activity, bone matrix production andosteoblast-specific gene expression were induced in normal human fibroblasts cultured with Repsox. The chemical compoundmediated directly converted osteoblasts (cOBs) were similar to human primary osteoblasts in terms of expression profilesof osteoblast-related genes. The cOBs abundantly produced bone matrix in vivo and facilitated bone regeneration after they
were transplanted into immunodeficient mice at an artificial bone defect lesion of femur.Conclusions: Taken together, thepresent procedure may realize direct conversion of human fibroblasts into transgene-free osteoblasts that may applicable toa novel strategy of bone regeneration therapy for bone diseases including jawbone cyst or tumor, and severe periodontitis.
Furthermore, this technique has advantages not only in cell therapy but also disease modeling, and drug discovery.

Special Lectures

K. YAMAMOTO1,2, T. YAMAMOTO1, T. KISHIDA2, O. MAZDA2, N. KANAMURA1

Symposium I

Applicability of neutral hypochlorous acid water for cleaning fixed orthodontic appliances
Y. NAGAMATSU, H. IKEDA, H. SHIMIZU
Division of Biomaterials, Department of Oral Functions, Kyushu Dental University, Kitakyushu, Japan

Poster Presentation

─ 81 ─

Rising Scientist Session

Objectives: For patients taking orthodontic treatment with fixed appliances, especially pediatric patients, it is not easy toclean narrow areas between a bracket and a wire and tooth surface around the bracket. For constructing a simple and safe
cleaning method, we focus on neutral hypochlorous acid water (NHW) due to its high microbicidal activity and no negative
effect on human enamel. In this study, we examined the bactericidal effect of NHW to a bracket ligated with a wire to evaluate
its applicability in dental practice.Methods: A Co-Cr alloy wire (2cm) was ligated with a metal or resin bracket using with apreformed ligature wire. A metal bracket bonded to an apatite pellets (10×10×2 mm) with an adhesive resin cement were also
prepared. After contamination by 15-h immersion in bacteria (Streptococcus mutans) suspension, each specimen was cleanedby brushing with an interdental brush, water flow washing or water jet washing with an oral irrigator using NHW (NHW30 or
NHW100: 30ppm or 100ppm). The treated specimen was ultrasonically cleaned in sterile phosphate-buffered saline for 5 min tocollect bacteria, and each extract solution was added to an agar medium, and incubated for 48 h at 37°C. After incubation, thetotal number of surviving bacteria on the specimens was calculated from CFU on the agar plate. As comparisons, the specimens
treated with tap water or commercial mouthwashes, Listerine or Concool were also tested.Results: The number of surviving
bacteria after water jet washing was significantly less than those after other treatments (p<0.05). The combination of NHW30
or NHW100 and water jet washing had a significantly higher bacteria removal effect than other combinations and commercial
mouthwashes (p<0.05).Conclusions: The water jet washing with NHW showed an excellent ability to remove Streptococcus
mutans. This finding suggests that NHW might be applicable for cleaning fixed orthodontic appliances contaminated with oral
bacteria.

Symposium II

032:

033:

Long Term Effects on Root Resorption: Conventional vs Mini-Screw Assisted Rapid Palatal Expansion
S. Mehta1, S. A. Arqub2, M. Lagravere3, C. Kuo2, A. Tadinada2, M. Upadhyay2, S. Yadav2
Developmental Sciences/Orthodontics, Marquette University School of Dentistry, Milwaukee, Wisconsin, UNITED STATES, 2Universityof Connecticut, Farmington, Connecticut, UNITED STATES, 3University of Alberta, Edmonton, Alberta, CANADA

1

Objectives: To evaluate the immediate and long-term effects of conventional and mini-screw assisted rapid palatal expansion
appliances on root resorption in comparison to controls using Cone Beam Computed Tomography (CBCT).Methods: A total of
180 CBCTs for 60 patients were assessed at three time-points T1 (initial), T2 (post-expansion), and T3 (debond) for three groups: i)Controls, ii) Rapid Palatal Expansion Appliance (RPE), and iii) Mini-screw Assisted Rapid Palatal Expansion appliance (MARPE)(Time-period T1 to T3: Controls: 2 years 7 months (n=19), RPE: 2 years 9 months (n=21), MARPE 2 years, 8 months (n=20). The
length of mesiobuccal, distobuccal, and palatal root of maxillary first molar, buccal root of maxillary first premolar, and second
premolar were measured. The inclination of maxillary first molar, inter-cuspal width (ICW), inter-root width (IRW), ratio of
ICW/IRW, maxillary skeletal width (MSW) were measured in all three groups at the different time-points.Results: Immediately
following expansion, both RPE and MARPE showed a significant increase in the molar inclination, ICW, ICW/IRW ratio, andMSW as compared to controls at T2. RPE showed a decreased palatal root length than MARPE when adjusted for age andtreatment time at post-expansion by 1.38 mm (95% CI: 0.17 mm, 2.60 mm, p<.05). However, the long-term comparison did notshow any significant difference for root resorption and expansion parameters between the three groups, except ICW/IRW ratio
which was higher in MARPE compared to controls at T3. A significant negative association was observed between the lengthof mesiobuccal root of maxillary first molar and molar inclination (beta= -0.025, 95% CI: -0.050 to 0.0008, p<.05). The amountof expansion at cuspal level (ICW) and root level (IRW) did not show a significant association with root resorption.Conclusions:
The long-term outcomes showed no difference in the amount of root resorption between the RPE, MARPE, and control groups.
Molar inclination showed a significant negative association with the length of mesiobuccal root of maxillary first molar.

034:

Identification and characterization of von Willebrand factor D and EGF domain as a novel extracellularmatrix protein in teeth
K. IWATA1, K. KAWARABAYASHI2, K. YOSHIZAKI3, A. SUGIMOTO1, S. FUKUMOTO4,5, T. IWAMOTO1
The Department of Pediatric dentistry and special needs dentistry, Tokyo medical and dental University , Tokyo, Japan , 2Departmentof Pediatric Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan, 3Section of
Orthodontics, Division of Oral Health, Growth and Development.Faculty of Dental Science, Kyushu University, Fukuoka, Japan ,1

4Department of Pediatric Dentistry, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan, 5Division of Pediatric
Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry, Sendai, Japan

Objectives: The process of tooth development is involved in the epithelial-mesenchymal interaction . Problems that arise in thisprocess affect the number, structure, morphology, and hardness of teeth, which in turn leads to developmental abnormalities
and diseases such as anodontia, congenital deficiency, morphological abnormalities, and hypoplasia. Therefore, it is importantto understand the molecular mechanisms of tooth development and its anomalies. Here, we aimed to analyze the expression
and function of von Willebrand factor D and EGF domain (Vwde) in tooth development.Methods: In order to find the tooth
specific gene, a bioinformatics method was performed with the expression tag database in NCBI. Then, to clarify the gene
expression in mouse tissues by RT-PCR, the cDNAs of organs including molar, brain, lung, heart, liver, kidney, and bone wereprepared from newborn ICR mice. Furthermore, the molar tooth germs were collected from embryonic day (E)11, E13, E14,
E15, E16, E18, post-natal day (P)1, P3, and P7 mice. Then, gene expression was analyzed by RT-PCR and northern blotting.
The frozen sections were prepared for each stage of tooth development, and Vwde expressing cells were analyzed by in situ
hybridization. In addition, the Vwde expression vector was transfected into a mouse dental epithelial stem cell line (M3H1) for
expression analysis by RT-PCR and for cell proliferation experiments by cell counting methods.Results: Vwde was found to be
specifically expressed in teeth of newborn mice compared to other tissues. Northern blotting revealed that the total length ofmRNA was about 7.0kbp. Furthermore, in situ hybridization showed that Vwde was expressed in the inner enamel epithelial
cells. Overexpression of the Vwde expression vector significantly reduced the cell proliferation in M3H1 cells.Conclusions:
We have demonstrated for the first time that Vwde is a new member of extracellular matrices and is preferentially expressedin teeth. Interestingly, Vwde was observed in inner enamel epithelial cells and inhibited the cell proliferation of M3H1 cells.
These results suggest that Vwde functions as a regulator for cell proliferation in dental epithelium. In future, elucidation of themolecular mechanism of Vwde may contribute to understanding of tooth development and diseases.

─ 82 ─

035:

High-fat diet-induced XAF1 exacerbates diabetes by promoting pancreatic β-cell apoptosis

1

Department of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan,
Department of Cell Biology and Pharmacology, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

2

Joint Symposium

Objectives: Pancreatic β-cell apoptosis may be involved in the onset and progression of type 2 diabetes mellitus (T2DM),
although its mechanism remains unclear. We previously demonstrated that macrophage-derived interferon (IFN) β induced
X-linked inhibitor of apoptosis–associated factor 1 (XAF1) expression in β-cells and accelerated β-cell apoptosis in vitro. Here,
we explored the effects of XAF1 on β-cell function and progression of diabetes in vivo.Methods: Pancreatic β-cell-specific XAF1
overexpressing (Xaf1 Tg) mice were generated. Xaf1 Tg mice and their wild-type (WT) littermates were fed either a normal
diet or a 40% or 60% high-fat diet (HFD). The effects of β-cell XAF1 on β-cell apoptosis and exacerbation of diabetes were
investigated.Results: Palmitic acid induced IFNβ expression in macrophages, and HFD intake promoted macrophage infiltrationin pancreatic islets, both of which co-operatively upregulated XAF1 expression in mouse islets. Furthermore, HFD-fed Xaf1 Tg
mice demonstrated increased β-cell apoptosis, lowered insulin expression, and impaired glucose tolerance compared with WT
mice fed the same diet. These effects were more pronounced in the 60%HFD group than in the 40%HFD group.Conclusions:
Pancreatic β-cell XAF1 expression was enhanced via HFD-induced, macrophage-derived IFNβ, which promoted β-cell apoptosis
and led to a reduction in insulin secretion and progression of diabetes. Since bacterial endotoxin also stimulates IFNβ
production from macrophages, it may indicate an association between low-grade inflammation-induced pancreatic β-cell XAF1
expression and exacerbation of diabetes, thus providing insight into the mechanism of β-cell mass reduction in diabetes.

Special Lectures

Y. NISHIMURA1, M. IWASHITA1, M. HAYASHI1, T. SHINJO1, T. ZEZE1, A. YAMASHITA1, T. FUKUDA1, T. SANUI1, T. SANO2, F.
NISHIMURA1

Symposium I

Inhibitory effects of β-Glycyrrhetinic acid on bacterial growth and biofilm formation by supragingival
plaque commensals
N. DEWAKE1, X. MA1, K. SATO2, S. NAKATSU2, K. YOSHIMURA2, Y. ESHITA2, H. FUJINAKA2, Y. YANO2, A. YOSHIDA3, N.
YOSHINARI1
1

Department of Periodontology, Faculty of Dentistry, Matsumoto Dental University, Shiojiri, Japan, 2Personal Health Care ProductResearch, Kao Corporation, Tokyo, Japan, 3Department of Oral Microbiology, Faculty of Dentistry, Matsumoto Dental University, Shiojiri,
Japan

Poster Presentation

─ 83 ─

Rising Scientist Session

Objectives: β-glycyrrhetinic acid (BGA), a major component of licorice extract, displays anti-inflammatory effects in humans.
The purpose of this study was to investigate the inhibitory effects of BGA on bacterial growth and biofilm formation by
supragingival plaque commensals.Methods: We first determined the minimum inhibitory concentration (MIC) of BGA against
oral bacteria. Then, we determined the MIC against biofilm formation by glucan synthesizing Streptococcus mutans andStreptococcus sobrinus. Next, the effects of BGA on coaggregation of Porphyromonas gingivalis and Streptococcus gordonii
and human supragingival plaque bacteria were evaluated. The human supragingival plaque samples were obtained from 12
healthy donors. All procedures were conducted in accordance with the guidelines of the ethics committee at the Faculty of
Dentistry, Matsumoto Dental University (No. 0295).Results: We first assessed the BGA MIC against oral bacteria associatedwith dental caries, gingivitis, and periodontitis. The MIC of BGA to prevent biofilm formation by S. mutans and S. sobrinus
was 1/4 to 2X the MIC for oral bacteria. BGA’s inhibitory effect on the coaggregation of P. gingivalis and S. gordonii was
confirmed at 1/2 to 1X the MIC of oral bacteria. In 11 out of 12 supragingival plaque samples, BGA significantly inhibited thebiofilm formation around the MIC of oral bacteria. The number of bacteria in the biofilm was significantly reduced at the same
concentration.Conclusions: BGA inhibited the growth of oral bacteria and biofilm formation. The same effect was observed
for bacteria in human supragingival plaque commensals. While antimicrobial agents do not penetrate the biofilm, BGA aroundthe MIC of supragingival bacteria significantly inhibited biofilm formation. In addition, inhibition of P. gingivalis and S. gordonii
coaggregation may inhibit supragingival colony formation and subsequent coaggregation and plaque maturation. Therefore,
BGA represents a candidate therapy for the prevention of periodontal disease by inhibiting the development and progressionof gingivitis.

Symposium II

036:

037:

Insulin resistance in vascular endothelial cells contributes to the exacerbation of periodontitis via
dysregulation of inflammation-induced VCAM-1 expression
T. ZEZE, T. SHINJO, Y. NISHIMURA, K. SATO, M. IMAGAWA, M. IWASHITA, A. YAMASHITA, F. NISHIMURA
Department of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

Objectives: The prevalence and severity of periodontitis are known to be higher and severer in subjects with diabetes. Recent
basic studies have shown that insulin resistance (IR) was also induced in the gingiva under diabetes. However, it remains
unclear how IR in gingiva-contributes to the exacerbation of periodontitis. In this study, we focus on vascular endothelial cells
which play an important role in the infiltration of inflammatory immune cells in response to periodontal infection. This study
aims to investigate the regulatory mechanisms of insulin and IR on inflammation-induced cell adhesion molecules (CAMs)
expressions in these cells, and to explore the possible mechanism underlying the deterioration of periodontitis by IR.Methods:
The regulatory effect of insulin on E. coli LPS- and TNFα- induced CAMs expression was confirmed by qPCR and Western blot
(WB) using TKD2, a cell line derived from murine renal small vascular endothelium. The signaling pathways were investigatedby using specific inhibitors for PI3K and MEK1 (wortmannin and PD98059). Cell adhesion assay was performed using THP-1, ahuman monocyte-derived cell line, and TKD2 cells.Results: Insulin pretreatment significantly suppressed E. coli LPS- and TNFαinduced up-regulation of VCAM-1, but not other CAMs such as ICAM-1 and E-selectin mRNA and protein expressions. This
regulatory effect of insulin was inhibited by wortmannin, but not PD98059. Consistent with the changes in VCAM-1 expression,
the cell adhesion between TKD2 and THP-1 cells was regulated by insulin, and the effect was inhibited by wortmannin but not
PD98059.Conclusions: It was suggested that IR in vascular endothelial cells may contribute to the exacerbation of periodontitis
by dysregulation of inflammation-induced VCAM-1 expression, thereby enhancing inflammatory cell infiltration.

038:

Dipotassium Glycyrrhizate inhibits LPS-induced alteration of cytoskeleton in gingival fibroblasts
T. YANAGISAWA, A. YOSHIDA, M. INAGAKI, S. SATONIPPON ZETTOC CO., LTD, Sagamihara, Japan

Objectives: Cytoskeleton is a key factor of maintenance in cell morphology and functions. It is known that Lipopolysaccharide(LPS) derived from periodontopathic bacteria disorganizes cytoskeleton in human gingival fibroblasts. In in vitro study forcosmetic research, the relation between cytoskeleton and elasticity development of skin is well known. Skin elasticity is
maintained by actin cytoskeleton generating cell tension. It is expected that similar mechanisms occur in gingival tissue. In
this study, we focused on actin cytoskeleton involved in cell tension development and investigated effects of Dipotassium
Glycyrrhizate from Glycyrrhiza glabra on Porphyromonas gingivalis (P.g)-LPS-stimulated human gingival fibroblasts.Methods:
We observed actin cytoskeleton using fluorescence microscope by immunofluorescence staining of the actin cytoskeletonin untreated human gingival fibroblasts (Control group) and cells treated with P.g-LPS (LPS group) or P.g-LPS + Dipotassium
Glycyrrhizate (LPS + Dipotassium Glycyrrhizate group). For estimation of cell tension, we performed a collagen gel contraction
assay, which is well known for evaluating the elasticity of skin in cosmetic fields. Polymerized collagen gels containing humangingival fibroblasts were cultured under several conditions: Control, LPS, LPS + Dipotassium Glycyrrhizate group. For assessmentof cell migration, we carried out scratch assay under several conditions: Control, LPS, LPS + Dipotassium Glycyrrhizate group.
Results: In immunofluorescence staining, the actin cytoskeleton change observed in LPS group was tended to be suppressed
in LPS + Dipotassium Glycyrrhizate group. In collagen gel contraction assays, the gel contraction was inhibited in LPS group.
However, compared with LPS group, gel contraction was not inhibited in LPS + Dipotassium Glycyrrhizate group. In scratch
assays, LPS + Dipotassium Glycyrrhizate group tended to accelerate cell migration.Conclusions: These results suggested that
Dipotassium Glycyrrhizate had moderating effects on P.g-LPS-induced alteration of actin cytoskeleton, maintained cell tension,and tended to influence cell motility in P.g-LPS-stimulated human gingival fibroblasts.

─ 84 ─

039:

The loss of IκBζ accelerates dentin formation and matrix gene expression

1

The Department of Periodontology and Endodontology, Tohoku University school of Dentistry, Sendai, Japan, 2Department of
Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan, 3Department of Biological Endodontics, Graduate Schoolof Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan, 4Department of Restorative Dentistry, Tohoku University
Graduate School of Dentistry, Sendai, Japan

Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

Rising Scientist Session

Objectives: Early primary tooth loss is often associated with a genetic disease or chromosomal abnormality, though rarely
occurs in individuals without a systemic disorder. Generally, periodontal disease with loss of teeth is considered to be associatedwith the presence of subgingival periodontopathic bacteria. This study, we examined subgingival periodontopathic bacteria
in children with early primary teeth loss as well as those without systemic disease.Methods: Gingival crevicular fluid andsubgingival plaque samples were collected from 12 children (5 patients, age 7.5 ± 1.1 years; 7 healthy subjects, 8.3 ± 2.1years).
Those were obtained with paper points from the deepest pocket in each patient and from the backmost molars in the healthy
subjects without a systemic disorder. Bacterial DNA was extracted from the specimens, then PCR analyses were performed for
detection of 10 major periodontal pathogens using specific primers.Results: The number of periodontopathic bacterial species
in the patient group was significantly higher as compared to the healthy group (6.2 ± 1.6 vs. 1.8 ± 1.6, P<0.01). Positive rates
were significantly higher in the patient group for Porphyromonas gingivalis (60.0% vs. 0.0%, P<0.05), Tannerella forsythia (80.0%
vs. 0.0%, P<0.05), Prevotella nigrescence (80.0% vs. 0.0%, P<0.05), and Eikenella corrodens (100.0% vs. 28.5%, P<0.05). Thus,
the number of red complex, orange complex, and green complex were significantly higher in the patients (1.4 ± 0.8 vs. 0.0 ±0.0, P<0.01; 1.8 ± 0.4 vs. 0.5 ± 0.5, P<0.01; 3.0 ± 0.7 vs 1.4 ± 1.1, P<0.05, respectively).Conclusions: The present results suggest
several different periodontopathic bacteria that may be associated with early primary tooth loss, which were detected withhigher frequency in the oral cavity of patients with early primary tooth loss as compared with healthy subjects.

Symposium II

Analysis of periodontopathic bacteria related to early primary tooth loss
Y. MIYAI, S. NAKA, M. MATSUMOTO-NAKANO

Symposium I

040:

Joint Symposium

Objectives: Enforced enhancement of H3K4me3 and H3K27ac by inhibiting histone demethylases and deacetylases is positively
linked with hard tissue formation by inducing matrix synthesis and osteo/odontogenic differentiation. However, the key
endogenous epigenetic modifier of odontoblasts to regulate the expression of the genes coding for dentin extracellular matrix(ECM) proteins has not been identified yet. IκBζ was originally identified as the regulator for NF-kB and recently regarded as theepigenetic modifier by independently on NF-kB. Therefore, the aim of this study was to delineate the roles of IκBζ for dentin
formation.Methods: The roles for IκBζ in dentin formation were explored by analyzing dentin phenotypes in IκBζ knockout
mice (IκBζ KO) and the roles of IκBζ for epigenetic status evoked by the knockdown of IκBζ were clarified in odontoblast-likecells.Results: IκBζ KO mice exhibited thicker dentin width and narrower pulp chamber with aged mice having more drastic
phenotypes. At 6 months old, fluorescent labeling exhibited that dentin synthesis was significantly accelerated in the incisorsof IκBζ KO mice. In molars of IκBζ KO mice, aggressive reactionary dentin adjacent to pulp horn was exhibited. Mechanistically,
Col1a2 and Col1a1 collagen genes were significantly increased in the odontoblasts rich fraction of IκBζ KO mice than that ofwild type in vivo. Human odontoblasts-like cells transfected with siRNA for IκBζ expressed higher COL1A2 and COL1A1 than thecells transfected with control siRNA in vitro. Furthermore, FLAG tagged IκBζ overexpression in odontoblast like cells showed
direct binding of IkBζ to COL1A2 promoter, suppressed COL1A2 expression, and activated the local chromatin status marked
with H3K4me3. Whole-genomic identification of H3K4me3 enrichments revealed that ECM and ECM organization-related
gene loci were selectively activated by the knockdown of IκBζ whereby resulted in the up-regulation of these gene expression.
Conclusions: IκBζ is the key negative regulator of dentin formation in odontoblasts since the deletion of IκBζ expression
enhanced dentin formation by inducing dentin ECM and ECM organization-related gene expression via altering the chromatin
status marked by H3K4me3. Therefore, IκBζ is a potential target for improving the clinical outcomes of dentin regeneration
therapies.

Special Lectures

H. YUAN1, S. SUZUKI1, H. TERUI2, S. HIRATA-TSUCHIYA3, E. NEMOTO1, K. YAMASAKI2, M. SAITO4, H. SHIBA3, S. AIBA2, S.
YAMADA1

Poster Presentation

─ 85 ─

041:

Hepatocyte growth factor shows antifibrotic effect on nifedipine-induced gingival overgrowth in vitro
model
K. YAMAZAKI1, H. IGARASHI-TAKEUCHI1,2, Y. NUMABE1
1

Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan, 2 Core Research Facilities
for Basic Science, Research Center for Medical Science, The Jikei University School of Medicine, Tokyo, Japan

Objectives: Nifedipine-induced gingival overgrowth is a gingival hyperplasia that occurs as a side effect in patients taking
nifedipine. The aim of this study is to establish an in vitro model of nifedipine-induced gingival overgrowth and confirm theantifibrotic effect of hepatocyte growth factor (HGF).Methods: Human gingival fibroblasts were cultured in DMEM/F-12 with
10% FBS and treated with 0.1, 1, 10 μg/ml nifedipine, 10 ng/ml IL-1β + 0.1, 1, 10 μg/ml nifedipine (N0.1, N1, N10, IL + N0.1,
IL + N1, IL + N10, IL + N0.1, IL + N1, IL + N1, IL + N10). The number of cells and type I collagen, TGF-β1, CCN2/CTGF and α-SMA
proteins were measured. After 10 and 50 ng/ml HGF were added simultaneously with the IL-1β and nifedipine, type I collagen,TGF-β1 and CCN2/CTGF were measured after 48 hours.Results: For type I collagen, TGF-β1 and CCN2/CTGF, there was asignificant increase at 48 hours after treatment in the N1 and IL + N0.1 groups. And, for type I collagen and CCN2/CTGF, there
was a significant difference in IL + N0.1 + 50HGF group compared to IL + N0.1 group. Also, the production of type I collagen was
significantly increased by the addition of anti-HGF antibody.Conclusions: A nifedipine-induced gingival overgrowth model wasestablished in vitro, and it was suggested that HGF had an anti-fibrotic effect in this model.

042:

Personalized medicine of oral implant therapy for otorhinolaryngological high-risk patients.
K. TAKAHASHI1, K. YAMAZAKI1, M. YAMAZAKI2, Y. BABA3
1

Division of Periodontics, Department of Conservative Dentistry, Ohu University School of Dentistry, Koriyama, Japan, 2Ohu University
Dental Hospital, Koriyama, Japan, 3Ohu University Hospital Otorhinolaryngology, Koriyama, Japan

Objectives: Maxillary sinus floor elevation surgery (SES) is a reliable technique. However, this treatment is not riskless of
postoperative complications such as maxillary rhinosinusitis and postoperative infection. We present different managementsof periodontitis patients with otorhinolaryngological high-risk for SES.Methods: Three periodontitis patients withotorhinolaryngological high-risk for SES were collected.Results: For a 48-year-old male with anatomical-structural impairments,
such as septal deviation, concha bullosa, the presence of Haller cell and nasal mucosal swelling by the nasal allergy, while nosinusitis, preoperative topical steroid and leukotriene receptor antagonist in addition to peri-operative antibiotic prophylaxis
has been performed and then his complication after SES was completely prevented. For a 69-year-old female with neutrophilicchronic rhinosinusitis and olfactory disorder, long-term low-dose macrolide therapy was performed with good outcome andthen implant treatment was successfully performed after SES. In contrast, for a 46-year-old male with eosinophilic chronicrhinosinusitis, SES were not performed because this patient showed the recurrence of nasal polyps after endoscopic sinus
surgery performed by a previous otorhinolaryngologist. The implant overdentures were applied alternatively for his oral
rehabilitation.Conclusions: We need to choose the optimal treatment regimen according to the pathological conditions ofparanasal sinus and the collaboration between dentists and otorhinolaryngologists is crucial to get precise diagnosis andpersonalized medicine for oral implant therapy of otorhinolaryngological high-risk patients.

─ 86 ─

043:

K. KAJIWARA1, S. TAMAOKI1, Y. SAWA2
1

Division of orthodontics, department of oral growth & development, Fukuoka Dental College, Fukuoka, Japan, 2Deparment of Oral
Function & Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

Joint Symposium

Objectives: The present study aims to examine the renal SGLT2 induction by the TLR2/4 ligand Porphyromonas (P.) gingivalis
lipopolysaccharide (Pg-LPS) in mouse diabetic nephropathy.Methods: Immunohistochemical study and tissue RT-PCR
analyses were performed on mouse kidneys in streptozotocin (STZ)-induced diabetic ICR mice (STZ-ICR), in healthy ICR miceadministered Pg-LPS (LPS-ICR), and in diabetic ICR mouse kidneys with Pg-LPS-induced nephropathy (LPS-STZ).Results: In thequantitative analysis of blood sugar levels, the mean time to reach 600 mg/dl was shorter in the LPS-STZ than in the STZICR kidneys. The rise in blood glucose levels was significantly steeper in the LPS-STZ than in the STZ-ICR kidneys. According tothese data the LPS-STZ model suggests a marked glucose intolerance. The expression of SGLT2 was significantly stronger in thewhole of the renal parenchyma of the LPS-STZ than in the LPS-ICR or in the STZ-ICR. The expression of SGLT2 was observed
both in the renal tubules and around the renal tubules, and in the glomeruli of the LPS-STZ kidneys. In the analysis by tissue
real-time PCR and cell ELISA, the expression of the SGLT2 gene and protein was significantly stronger in the LPS-STZ than in
the LPS-ICR or in the STZ-ICR. There were no differences in the renal SGLT2 production in the LPS-ICR and the STZ-ICR kidneys.
Conclusions: Abnormally high renal expression of SGLT2 occurs in diabetic kidneys with P. gingivalis LPS. Periodontitis may be
an exacerbating factor in diabetic nephropathy as well as in diabetes.

Special Lectures

Abnormal expression of SGLT2 in the kidney of a P. gingivalis LPS-induced diabetic nephropathy mousemodel

Symposium I

Anti-inflammatory and Anti-oxidative Effects of Febuxostat on Periodontitis Rats Model
N. NESSA1,2, M. KOBARA2, T. ADACHI1, T. YAMAMOTO1, T. NAKATA2, N. KANAMURA1
1

Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan, 2Pathological Science, Department of Clinical Pharmacology,
Kyoto Pharmaceutical University, Kyoto, Japan

Poster Presentation

─ 87 ─

Rising Scientist Session

Objectives: Periodontal disease is quite prevalent and affects about 20-50% of global population. Periodontitis is a chronic
inflammatory disease of the supporting structures of the teeth. When periodontal pathogens enter into the blood stream, suchlifestyle diseases develop. Febuxostat, a xanthine oxidase inhibitor, exerts anti-inflammatory and antioxidant effects. This study
aims to evaluate the effects of febuxostat on periodontitis in a rat model.Methods: Wistar rats were used in this study being
divided randomly into 3 groups: control, periodontitis, and febuxostat-treated periodontitis groups. Experimental periodontitis
was induced by placing the ligature wire around the upper 2nd molar of the rat; the administration of febuxostat (5 mg/kg/day) was then initiated. After 4 weeks, alveolar bone loss was evaluated by micro-computed tomography and methylene blue
staining. The expression of bone resorption inhibitor osteoprotegerin (OPG), in gingiva was detected by quantitative RT-PCR
and immunological staining. Tartrate-resistant acid phosphatase (TRAP) staining was used to assess the number of osteoclastsin gingival tissue. Quantitative RT-PCR and immunological staining were used to examine the inflammatory cytokines
expression. Oxidative stress in gingiva was also evaluated by the expression of 4-hydroxy-2-nonenal (4-HNE), and 8-hydroxy2-deoxyguanosine (8-OHdG). In addition, blood pressure and glucose tolerance were examined to clarify the systemic effectsof periodontitis.Results: In rats with periodontitis, alveolar bone resorption increased with reductions in OPG; the number of
TRAP positive osteoclasts grew. The expression of TNF-α, IL-1β, and 4-HNE, and 8-OHdG in the gingiva was up-regulated in the
periodontitis group and treatment with febuxostat significantly reduced alveolar bone loss, proinflammatory cytokine levels,and oxidative stress. It also attenuated periodontitis-induced glucose intolerance and blood pressure elevation.Conclusions:Febuxostat prevented the progression of periodontitis and associated systemic effects by suppressing proinflammatory
mediators and oxidative stress. Febuxostat is extremely promising for drug repurposing in dentistry.

Symposium II

044:

045:

Effects of smoking and smoking cessation on human gingival and lung fibroblasts
H. TAKEUCHI-IGARASHI1,2, T. TACHIBANA2, Y. MANOME2, Y. NUMABE1
1

Department of Periodontology, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan, 2Core Research Facilities
for Basic Science, Research Center for Medical Science, The Jikei University School of Medicine, Tokyo, Japan

Objectives: The adverse effects of smoking have been widely reported. However, the effects of smoking cessation have notbeen fully elucidated. The purpose of this study was to investigate the effects of smoking and the changes in the repair period
due to smoking cessation in a time-dependent manner and to compare the results with those of human gingival fibroblasts(HGF) and human lung fibroblasts (HFL1).Methods: HGF cells were obtained from human normal gingiva. Human lung fibroblast
cell line was purchased. When cells were cultured until confluence, medium was replaced 1μg/ml nicotine contained medium
for 24 hours. Then cells were washed by medium and replaced non-nicotine fresh medium until 48hours. Cell proliferations
were measured by MTT assay. The migration of cells in response to eliminated nicotine for 48hours was investigated in an invitro wound healing model. The cell cultures were analysed by morphorogicaly examination under phase-contrast microscope
and transmission electron microscope (TEM).Results: It was detected that ability of cell migration of smoking cessation groupwas significantly increased comparing with smoking group in both cells (p<0.001). Smoking group ware significantly decreased
comparing with control group and smoking cessation group (p<0.05). Following 24hours nicotine stimulation, there wereobserved a lot of vacuolization in cytoplasm by phase-contrast microscope and TEM. However it trended to disappear timedependently from cytoplasm by removing nicotine.Conclusions: Our study demonstrated the cell damaging effects of smoking
in HGF and HFL1. On the other hand, damage caused by smoking still remained in cell, we also indicated ability of the cell
repairing effect of smoking cessation. This work was supported by Grant-in Aid for Scientific Research (C) Grant number
20K10280 from the Japan Society for the promotion of Science.

046:

Profiles of Mastication Predominance and Masticatory Performance in Kennedy Class I Patients
K. KINOSHITA1, K. OKI2, Y. OGINO2, Y. TSUKIYAMA3, Y. AYUKAWA1, K. KOYANO4
1

Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka,
Japan, 2Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan,
3
Section of Dental Education, Division of Oral Biological Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan,
4
Division of Advanced Dental Devices and Therapeutics, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

Objectives: Missing posterior teeth declines masticatory performance and may cause mastication predominance. Previous
studies have shown that Kennedy class I (KCI) patients have mastication predominance, but it has not been well defined. The
aim of this study was to investigate the effect of remaining posterior teeth and removable partial denture (RPD) treatment
on mastication predominance and masticatory performance in KCI patients.Methods: The subjects in this study were thepatients who visited the Department of Prosthodontics, Kyushu University Hospital. The following patients were included as
the subjects: patients over 20 years old who had missing posterior teeth bilaterally (KCI) in one jaws and complete dental archwith natural teeth or fixed dental prostheses in opposite jaws; patients who were scheduled for bilateral RPD treatments. KCI
patients who had difference in the number of posterior teeth between the right and left sides (D+) and KCI patients who had
no difference (D-) were registered in this study. Healthy dentate (HD) subjects were also enrolled as control group. Mastication
predominance was defined by mastication predominance index (MPI; range 0-100%) using electromyography. MPI andmasticatory performance were compared statistically between pre and post RPD treatment.Results: Pre-MPI in KCI D+, but not
in KCI D-, was significantly higher than in HD. RPD treatment significantly improved MPI and masticatory performance in KCI
D+ and D- patients. However, there was a significant difference in mastication performance between both groups of KCI andHD, regardless of RPD treatment.Conclusions: Mastication predominance in KCI patients was thought to be influenced by thedifference of the number of remaining posterior teeth between left and right side. RPD treatment could improve mastication
predominance and masticatory performance in KCI patients, although the latter was not greater than HD group.

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047:

Bacterial flora evaluation using DNA array chip and real-time PCR
GC CORPORATION, Tokyo, Japan

Joint Symposium

Objectives: Periodontal disease is an inflammatory condition caused by a bacterial infection. Generally, periodontopathic
bacteria and residental bacteria are well-balanced. However, some periodontal risks cause the disruption of a balance of
periodontopathic bacteria and residental bacteria, which leads to pathogenesis or progress of the periodontal disease. Since
more than one bacterial species are associated with periodontal disease, it is important to evaluate the bacterial flora of thepatient, in order to manage the periodontal disease. By the bacterial test using a DNA array chip (Mitsubishi Chemical, Japan),periodontopathic bacteria could be detected at once. In this study, we detected periodontal disease bacteria by DNA chipand real-time PCR for the purpose of quantification.Methods: Three patterns, A to C, of the standard bacterial mixture were
tested as samples (n = 3). DNA was extracted from the Standard bacterial genome mixture using QuickGene-810(FUJIFILM,
Japan). DNA extracted from the genome mixture was fluorescence-labeled by PCR method. Fluorescence-labeled DNA washybridized with the DNA array chip, and fluorescence intensity was measured by Genopal Reader (Mitsubishi Chemical, Japan).
5 bacterial species(Pg, Td, Tf, Pi, Aa) were also evaluated by real-time PCR.Results: In all mixtures, differences in fluorescenceintensity were observed depending on the presence or absence of the mixture, and the same trend as in the real-time PCR was
confirmed. In the standard bacterial genome mixtures A to C, the fluorescence intensities of some bacteria disappeared on the
DNA array chip, but this was because they were below the detection limit. In the case of Fusobacterium species, fluorescence
was also detected in non-mixed species. It is possible that cross-hybridization occurred when bacteria were present in thesample, and fluorescence was detected even in non-mixed species.Conclusions: The real-time PCR method is a generally known
quantitative test method for bacterial tests. It can be thought that quantitative detection is possible even in measurements
using the DNA array chip method. The DNA array chip method can measure multiple periodontal disease bacteria at one time,and it is expected to know more detailed periodontal condition than the real-time PCR method for several bacterial species.

Special Lectures

Y. KADOTA, E. ARIMA, S. HOTTA, F. FUSEJIMA

Symposium I

Effect of Glass Fiber Sleeve for Reinforcement of Flared Root
S. YOSHIMATSU, W. KOMADA, R. NEMOTO, S. OISHI, R. TSUKAHARA, S. OMORI, K. NOZAKI, H. MIURA
Fixed Prosthodontics, Tokyo Medical and Dental University, Tokyo, Japan

Rising Scientist Session

Objectives: Endodontically treated tooth with flared root often occurs root fracture because of its weakened root canal wall. It
has been reported that composite resin core tends to occur horizontal root fracture due to stress concentration in the cervicalarea. The aim of this study is to evaluate to reinforce the cervical area in flared root canal with glass fiber post and glass fiber
sleeve in the case of composite resin core.Methods: Six extracted bovine mandibular incisors free of cracks and fractures wereprepared. They were endodontically treated and uniformly shaped to simulate human maxillary central incisor with flared roots.
Cores were made on plaster models by indirect method. One group was used composite resin core (DC core automix, Kuraray
Noritake Dental) with glass fiber post (Clearfil Fiber Post II, Kuraray Noritake Dental) inserted glass fiber sleeve (i-TFC sleeve,
Sun Medical) inside (PS), while another group was used only composite resin core (RC). Crowns made of lithium disilicate wereused. Cores and crowns were cemented with dual-cure resin cement. Four strain gauges were attached to surfaces on eachspecimen: cervical area of root and crown, both in labial side and palatal side. The surface strain on each cervical area wasmeasured in loading-test, and the data was analyzed statistically by t-test at a statistical significance of 0.05.Results: The surface
strain of root in labial side was -561.18 (16.67) με in PS group, and -943.98(150.53) με in RC group. That of root in palatal side
was 450.85(40.93) με in PS group, and 748.45(113.09) με in RC group. Both in labial side and palatal side, the strains in PS groupwere significantly lower than RC group.Conclusions: This result suggests that it is effective to use glass fiber post and glass fiber
sleeve with composite resin core for reducing strain concentration in cervical area.

Symposium II

048:

Poster Presentation

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049:

The Influence of Zirconia Tube for Stress in Endodontically-Treated Molar
S. OISHI, W. KOMADA, D. KONDO, R. TSUKAHARA, S. YOSHIMATSU, Y. YOKOSUKA, S. OMORI, K. NOZAKI, H. MIURA
Fixed Prosthodontics, Tokyo Medical and Dental University, Tokyo, Japan

Objectives: Endodontically-treated molars are usually restored with post and core systems for the final restoration. Somestudies reported that tooth restored using composite resin core increased stress concentration at the cercival region of the
tooth. This study aims to evaluate the surface strain at the cervical region of molar restored using composite resin core witha prefabricated zirconia tube.Methods: Reproduction models of human mandibular molars with prepared post spaces wereused in this study. The roots that were duplicated with composite resin were used as experimental teeth. Three types of corebuild up systems were used: composite resin core (RC), composite resin core with fiber posts (FC) and composite resin withthe zirconia tube (ZC). Each group consisted of 8 specimens. Crowns made of yttria partially stabilized zirconia were cemented
with dual-cure resin cement. Four strain gages were attached to surfaces on each specimen: cervical region of root and crown,
both in buccal side and lingual side. The surface strain at each cervical region was measured in static loading test, and thedata was analyzed statistically.Results: In the case of static loading to the buccal cusp inner slope, ZC showed a significant
lower strain than RC in the crown buccal and FC in the root. As a result of the experiment in the central fossa, in the crown ZC
showed a significant lower strain than RC. In the root lingual FC showed a significant greater strain than RC and ZC. As a resultsof the experimental in the distal marginal ridge, ZC showed a significant lower strain than RC in the crown and FC in the root.
Conclusions: This study indicated the zirconia tube reduce stress at the cervical region in the molar restored using composite
resin core.

050:

New mechanism of odontoblast differentiation
H. NAKAZATO1, S. ONODERA2, N. AIDA2, T. AZUMA2, M. FURUSAWA1
1

Department of Endodontics, Tokyo Dental College, Tokyo, Japan, 2Department of Biochemistry, Tokyo Dental College, Tokyo, Japan

Objectives: Tooth developments is initiated by oral epithelium and adjacent mesenchyme. The phase of development relies
on several signals such as BMPs, Hedgehogs and WNTs for cross interaction of epithelium and mesenchyme. In this study, we
aimed to uncover the signal of late differentiation stage of mesenchymal cells.Methods: The epithelium and mesenchyme
derived from first molar of newborn C57BL/6J were isolated by enzymatic reaction. The isolated mesenchymal were cultured
each day and checked Dspp and Dmp1 gene expression by RT-PCR. RNA-seq was performed with day 0 and day 2 cultured
isolated mesenchymal cell to identify significantly downregulated gene after isolation. The genes identified by RNAseq werecloned into lentiviral vectors and produce each lentivirus. Dental mesenchymal cells (Dmc) and human dental pulp cells (HDP)
were transfected with these lentiviral vectors and were cultured in odontoblastic differentiation medium with or without FGF4,
FGF9, and CHIR99021 for 7 days. Odontoblastic gene expressions were analyzed by qRT-PCR.Results: Dspp and Dmp1 expression
were decreased after isolation. Pearson analysis of RNA-seq data showed different expression patterns between day0 and day2
samples. Immediate early genes (Egr1, Egr2, Fos, Fosb and Jun), Notch-related genes (Hey1), and Hedgehog-related genes (Gli1)
expression were remarkably decreased after isolation. In the isolated Dmc cultured in basic medium with FGF4, FGF9, andCHIR99021, the expressions of Egr1, Fosb, and Gli1 were increased, while Egr2, Gli1, and Hey1 expressions were increased in
HDP. We found that forced expression of all genes described above were required to increase odontoblast markers in Dmc and
HDP.Conclusions: Mesenchymal cells are regulated by multiple factors including Notch pathway, Immediate early genes, andHedgehog pathway.

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051:

Evaluation of Inflammatory Changes During Wound Healing Process in Caries-Stimulated Dental Pulp
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan

Joint Symposium

Objectives: Inflammatory process in caries-stimulated pulp has not been clarified how it may affect the outcome of vital
pulp therapy. This study aimed to establish rat reversible pulpitis model by caries stimulation and evaluate inflammatorychanges after pulp capping.Methods: Animal studies were conducted under the approval of Institutional Animal Care and
Use Committee of Osaka University Graduate School of Dentistry (No. R-01-017-0). Caries was induced by 5-days continuous
inoculation of Streptococcus mutans into oral cavity of 18-day-old SD rats and feeding with cariogenic diet. After confirming
moderate caries progression on maxillary molars by micro-CT (carious lesion between 1/3-2/3 of dentin thickness),immunohistochemical staining against TLR2, PCNA (Abcam, UK) and double immunofluorescence staining of CD68 (Bioss,
USA), CD206 (Abcam) were performed to evaluate initial inflammation induced by caries. To further investigate inflammatorychanges, direct pulp capping using ProRoot MTA (Dentsply-Sirona, USA) was performed in moderate caries tooth, thenmacrophage and PCNA distribution were investigated on day 1, 3, 7, 14 post capping. CD68(+)/CD206(-) cells are consideredas M1 macrophages, while CD68(+)/CD206(+) cells are M2 macrophages. Sound tooth was used as control group.Results:
Expression of TLR2 and PCNA were observed in the moderate caries group in the pulp. M2 macrophages were predominant
in the moderate caries induced inflamed pulp before capping. During wound healing process after capping, M2 macrophages
and PCNA cells showed maximum population on day 3, and started to decrease after day 7 in caries group. Significant higher
population of M2 macrophages and PCNA-positive cells were observed on day 3 compared with sound group (p<0.05, Student’s
t-test), which indicated inflammatory changes in caries group might differ from sound tooth during wound healing process.
Conclusions: We successfully established caries-stimulated reversible pulpitis model for pulp capping. M2 macrophages could
play pivotal role in wound healing process of reversible pulpitis.

Special Lectures

H. HUANG, M. OKAMOTO, M. WATANABE, S. MATSUMOTO, K. MORIYAMA, S. KOMICHI, Y. TAKAHASHI, M. HAYASHI

Symposium I

Induction of dental pulp cells into periodontal ligament-like cells using epigenetics
S. TAKAHASHI1, K. YOSHIDA1, D. PAUDEL1, D. ARIWANSA1, A. KHURELCHULUUN2, A. ONISHI1, T. MORIKAWA1, D. HIRAKI3, F.
HARADA2, O. UEHARA4, J. SATOU1, H. NAGAYASU2, Y. ABIKO1
1Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 2Divisionof Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 3Division of Oral andMaxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 4Division of Reconstructive Surgery
for Oral and Maxillofacial Region, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 5Division of Disease
Control and Molecular Epidemiology, School of Dentistry, Health Sciences University of Hokkaido

Poster Presentation

─ 91 ─

Rising Scientist Session

Objectives: Since dental pulp stem cells are present, application to regenerative medicine is expected, but the ratio of dental
pulp stem cells in the entire dental pulp (DP) is as low as a few percent. When applying DP to periodontal ligament regeneration,
it is desirable to apply the entire DP including stem cells. In this study, we created an epithelial-mesenchymal cell population
which express the periodontal ligament-related genes, consist of whole DP, epithelial cell rests of Malassez (ERM), and humanumbilical vein endothelial cells (HUVEC).Methods: ERM was dedifferentiated with 5-Azacytidine, a DNA methyltransferase
inhibitor, and valproic acid, a histone deacetylase inhibitor, to produce progenitor stem-like cells (Pro-DSLCs). The DP / ProDSLCs / HUVEC cell populations were co-cultured in mesenchymal stem cell medium for 1 week to induce periodontal ligamentlike cells. mRNA expression analysis was performed on periodontal ligament-related genes and mesenchymal stem cell positive/ negative genes by the qPCR method. DNA methylation analysis was performed by the qMSP method.Results: The significant
increased mRNA expression of the periodontal ligament gene Msx1 and the mesenchymal stem cell positive gene Cd29 wasobserved in the DP / Pro-DSLCs / HUVEC co-culture group as compared with the DP single culture group (p < 0.05, Steel test).
The significant decrease in the methylation levels of Msx1 and Cd29 was observed in the DP / Pro-DSLCs / HUVEC co-culturegroup as compared with the DP single culture group (p <0.01, chi-square test).Conclusions: It was suggested that the DP / ProDSLCs / HUVEC co-cultured cell population can be induced into periodontal ligament-like cells by methylation alteration.

Symposium II

052:

053:

Dentin-pulp complex tissue regeneration by three-dimensional cell sheet engineering
H. YAN1, M. OSHIMA1, R. RAJU2, S. RAMAN1, A. WASKITHO1, K. OKURA1, Y. MATSUKA1
1

Department of Stomatognathic Function and Occlusal Reconstruction, University of Tokushima, Tokushima, Japan, 2Department ofOral Disease Research, National Center for Geriatrics and Gerontology, Obu, Nagoya, Japan

Objectives: To regenerate dentin-pulp complex by scaffold-free technique, we designed a composite cell sheet which consistsof dental pulp cells (DPCs) layered over odontogenic differentiated DPCs (OD cells).Methods: We compared the regenerative
capacity of composite cell sheet to two single cell sheets containing either DPCs or OD cells. And we confirmed the composite
cell sheet by histological, immunohistochemistry (IHC), and Scanning Electron Microscope (SEM). A carrier tooth was preparedby removing the enamel, dentin and pulp to mimic tooth decay. Following which carrier tooth was covered with one of thethree cell sheet types and transplanted into subrenal capsule of ICR mice to check for ectopic regeneration. After 8 weeks,transplants were analyzed using H&E staining, Micro-computed tomography (CT) and IHC.Results: The results in H&E staining
and IHC of composite cell sheet indicated that were successful in layering two type of cells combine in one cell sheet. And thefindings in Micro-CT images showed hard tissue formation in the composite cell sheet group. Regenerated hard tissue formation
was significant in composite cell sheet group compared to the single cell sheet groups as tested by One-way ANOVA, Tukey
HSD test (*p< 0.05, **p< 0.001). Furthermore, histological and IHC analysis demonstrated disciplined dentin-pulp-like tissue
formation in the composite cell sheet group resembling natural tooth.Conclusions: In conclusion, our composite cell sheet
enables to regenerate an organized three-dimensional structure comparable to that of natural teeth. The successful capping of
the cavity by the regenerated dentin-pulp complex by our composite cell sheet can be a significant contribution to the future
dental medicine. Our research aids as a guide for the future prospects in the field of regeneration with multiple complex tissue
types.

054:

Effect of psychological stress on oral microbiome: A metagenomic and bioinformatic analyses
D. PAUDEL1, Y. KURAMITSU2, O. UEHARA3, T. MORIKAWA1, T. KITAGAWA1, K. YOSHIDA1, Y. ABIKO1
1Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan, 2Advanced
Research Promotion Center, Health Sciences University of Hokkaido, Japan, 3Division of Disease Control and Molecular Epidemiology,
School of Dentistry, Health Sciences University of Hokkaido, Japan

Objectives: Various oral diseases are associated with psychological stress. However, limited information about the effectof psychological stress on the oral environment has been shown. The objective of this study is to investigate the effect of
psychological stress on oral microbiomes using a rat model of chronic restraint stress.Methods: Sprague Dawley rats weredivided into the control and stress groups. The stress group rats underwent restraint stress by enclosing them in a plastic tube
for 4 hours daily for 1 month. The stress level was confirmed with various stress markers after 1 month. The rats were sacrificed
to collect oral swabs. The bacterial DNA was extracted and used to perform metagenomic analyses using 16S rRNA sequencing.
The sequencing data was analyzed using QIIME 2 software. A linear discriminant analysis (LDA) effect size (LEfSe) tool was used
to analyze bacterial population discrepancy between two groups. The alpha and beta diversity were analyzed. The PICRUSt2
tool and STAMP software were used to predict the alteration in microbial functional pathways.Results: The stress markers suchas low body weight, reduced activity in the open arm of the elevated plus-maze, high adrenal gland weight, and high serum
corticosterone level confirmed high-stress level in stress group rats as compared to control. Metagenomic analyses showedsignificantly reduced alpha diversity of oral microbiome in stress group as compared to control (Kruskal-Wallis test, p<0.05). The
LEfSe analysis showed 10 significantly altered bacteria such as Facklamia, Corynebacterium, Prevotella, and others between
two groups. PICRUSt2 and STAMP analysis showed 37 significantly altered functional pathways between the stress group andcontrol (Welch's t-tests, p<0.05).Conclusions: The present study showed that psychological stress significantly affected the oral
microbiome. These findings might be helpful in understanding the pathogenesis of stress-related oral diseases.

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C. HAYASHI, Y. WATANABE, K. KAWAKAMI, M. TOYODA, Y. NAKAO, K. YOTSUMOTO, H. YAMATO, T. SHINJO, T. SANUI, T.
FUKUDA, F. NISHIMURA
Departmen of Periodontology, Division of Oral Rehabilitation, Faculty of Dental science, Kyushu University, Fukuoka, Japan

Joint Symposium

Objectives: Human MSCs from gingiva (hGMSCs) are easier to isolate, and appear to secret higher amounts of exosome thanother MSCs. We recently demonstrated that exosome from TNF-α treated GMSCs increased the level of miR-1260b andinhibited periodontal bone loss (Nakao Y, et al., Acta Biomater, 2021). We further screened novel miR-1260b targeting genes by
database analysis and found that it could be associated with ER stress by targeting ATF6β. It has reported that ER stress-related
genes were up-regulated in periodontal tissue (Yamada H, et al. J Periodontal Res., 2002). In this study, we investigated thetherapeutic effect of miR-1260b in periodontal disease by targeting ATF6β-mediated regulation of ER stress.Methods: Human
periodontal ligament cells (hPDLCs) were transfected with miR-1260b mimic to validate miR-1260b-mediated inhibition of
ATF6β. The effect of miR-1260b on inflammatory bone loss were examined using mouse ligature-induced periodontitis model
under an institutionally approved animal research protocol (Kyushu University, #A21-131-0). The expression levels of ATF6β inmice were compared by qRT-PCR and immunohistochemistry, and alveolar bone loss was analyzed by Micro CT. To validate theeffect of miR-1260b on osteoclastogenesis, miR-1260b mimic transfected THP-1 cells were stimulated with M-CSF and RANKLfor osteoclast differentiation and the number of TRAP-positive cells were counted.Results: Transfection of miR-1260b mimic
inhibited ATF6βexpression and knock down of ATF6β decreased expression of RANKL mRNA in hPDLCs. Increased expressionof ATF6β was observed in the ligated periodontal tissue and local injection of miR-1260b mimic decreased periodontal bone
resorption in mice. The number of TRAP positive cells were decreased in miR-1260b mimic transfected THP-1-differentiatedosteoclasts.Conclusions: miR-1260b inhibited osteoclastogenesis and periodontal bone loss by targeting ATF6β-mediatedregulation of RANKL.

Special Lectures

Exosomal miR-1260b derived from TNF-α-treated hGMSCs inhibits periodontal bone loss by targeting
ATF6β-mediated regulation of RANKL

Symposium I

Exosomes derived from GMSCs stimulataed with TNF-a and IFN-a promote M2 macrophage polarization
via enhanced CD73 and CD5L expression
Y. WATANABE, C. HAYASHI, K. KAWAKAMI, M. TOYODA, Y. NAKAO, K. YOTSUMOTO, H. YAMATO, T. SHINJO, T. SANUI, F.
NISHIMURA
Departmen of Periodontology, Division of Oral Rehabilitation, Faculty of Dental science, Kyushu University, Fukuoka, Japan

Poster Presentation

─ 93 ─

Rising Scientist Session

Objectives: MSC-derived exosome plays a central role in the cell-free therapeutics and recent studies indicated that exosomal
contents can be customized under disease-associated microenvironments. We recently demonstrated that preconditioning of
human gingival tissue-derived MSCs (hGMSCs) with TNF-α was ideal for the treatment of periodontitis by enhancing exosomal
CD73 expression which induced anti-inflammatory M2 macrophage polarization (Nakao Y, et al., Acta Biomater, 2021. The aimof this study was to investigate more efficient cytokine stimulation of hGMSCs for the exosome-mediated M2 macrophage
polarizationand to explore the detailed molecular mechanisms.Methods: hGMSCs were isolated from human gingival tissues
under the approved of Institutional Review Board (IRB) protocol at Kyushu University Hospital (#2019-374). After pre-treatmentof the cells with 100 ng/mL of TNF-a/INF-a or 50 ng/mL of TNF-a/IFN-a for 48h, exosome from hGMSCs were isolated fromthe serum-free conditioned media using the MagCapture™ Exosome Isolation Kit PS. Macrophages were obtained by culturing
CD14+ monocytes with M-CSF isolated from PBMC. The effect of GMSC-derived exosomes on M2 macrophage polarization
were analyzed by FACS.Results: TNF-α combined with IFN-a stimulated hGMSCs-derived exosome more effectively promoted
the polarization of M2 macrophage than TNF-a or IFN-a alone. TNF-a/IFN-a stimulation induced nuclear accumulation of HIF-1aand enhanced mRNA expression of CD73 in hGMSCs. Transfection of HIF-1a expression plasmids in hGMSCs dose dependently
increased exosomal CD73 expression, while knockdown of HIF-1a decreased exosomal CD73 expression. TNF-a/IFN-astimulation
also upregulated the expression of CD5L, which was a known M2 driver.Conclusions: Compared with single cytokine stimulation(TNF-a or IFN-a), hGMSC-derived exosome from combined stimulation (TNF-a/IFN-a) significantly promoted M2 macrophage
polarization. TNF-a and IFN-a treatment resulted in enhanced expression of CD73 through induction of HIF-1a in hGMSCs.
Increased expression of CD73 and CD5L in hGMSC-derived exosome was essential for M2 macrophage polarization.

Symposium II

056:

057:

Characterization of neural crest-derived cells for application in bone regenerative medicine
K. SHINOMIYA, A. YAMADA, R. KAMIJO
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan

Objectives: The neural crest is formed on both sides of the neural plate by invagination in the embryonic period, after which
neural crest cells migrate throughout the embryo as neural crest-derived cells. It has been shown that some neural crest-derived
cells remain in colonization destination tissue as somatic stem cells and have pluripotency. To obtain tissue stem cells fromneural crest-derived cells as a resource for bone regeneration high efficiently, it is important to identify target cell propertiesand determine suitability for use as tissue stem cells. For this purpose, a new analysis method termed single cell RNA-sequence(scRNA-seq) was developed and found capable of detecting factors involved in transcriptional regulation of individual cells.
Methods: Mouse neural crest-derived cells were obtained from P0-Cre/GFP mice expressing GFP under control of the myelin
protein 0 (P0) gene promoter, which is specifically expressed in neural crest cells. scRNA-seq analysis was performed using achromium single-cell gene solution.Results: Nearly all cells collected from mouse inferior turbinate specimens after culturing
for 14 days were GFP-positive neural crest-derived cells. Furthermore, treatment with mineralization medium containing BMP-2
increased the gene expression of ALP and osteocalcin, markers of osteoblast differentiation. Next, based on the gene expressionof approximately 1000 cells, two-dimensional mapping using uniform manifold approximation and projection (UMAP) analysis,
also cell clustering analysis were performed, with a total of 13 clusters observed. Those results indicated that expression ofgenes characteristic of tissue stem cells was particularly present in clusters specifically expressing Sox2. Furthermore, they
revealed cell membrane surface proteins specifically expressed in clusters with specific Sox2 expression.Conclusions: We willconduct additional studies to identify cell surface proteins in clusters specifically expressing Sox2, then purify and apply themfor bone regenerative medicine.

058:

The effect of JNK inhibition on the osteoblastic differentiation of periodontal ligament stem cells and theregeneration of periodontal tissue in vivo
H. KANEKO1, D. HASEGAWA2, T. ITOYAMA2, S. YOSHIDA2, A. TOMOKIYO2, S. HAMANO1,3, H. SUGII1, H. MAEDA1,2
1
Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, Fukuoka, Japan., 2Division ofEndodontology, Kyushu University Hospital, Fukuoka, Japan, 3 OBT Research Center, Faculty of Dental Science, Kyushu University,
Fukuoka, Japan

Objectives: Few clinical treatments to regenerate periodontal tissue lost due to severe endodontic and periodontal disease
have yet been developed. Therefore, the development of new treatment methods for the regeneration of periodontal tissue
is required. The purpose of this study was to evaluate whether a JNK inhibitor, SP600125 is effective for periodontal tissue
regeneration.Methods: An immortalized human periodontal ligament (PDL) cell line, 2-23 cells showed PDL stem cell-like
properties, such as high growth capacity, multipotency, and the expression of stem cell-related surface markers (Hasegawa
et al., J Cell Physiol. 2018). Alizarin red S staining, quantitative RT-PCR analysis, and western blotting analysis were performed
to determine whether SP600125 affected osteoblastic differentiation of 2-23 cells and intracellular signaling. The effectof SP600125 on the regeneration of alveolar bone was assessed by using a rat periodontal defect model. Two weeks after
application of SP600125, the recovery of periodontal defects was evaluated using micro-CT scans and histological analysis.
Results: SP600125 promoted Alizarin red S-positive mineralized nodule formation and the expression of osteoblast-related
genes such as osterix, bone sialoprotein, and osteopontin in 2-23 cells under osteogenic conditions. This inhibitor upregulated
BMP2 expression and phosphorylation of Smad1/5/8 in 2-23 cells under the same conditions, while it did not affectphosphorylation of Erk1/2. SP600125 induced regeneration of alveolar bone compared with the control at two weeks after
application into periodontal defects, as well as reformation of periodontal ligament tissues.Conclusions: This study suggeststhat inhibition of JNK signaling promoted osteoblastic differentiation of HPDLSCs, probably through increased expressions of
BMP2 and the phosphorylation of Smad1/5/8, leading to the regeneration of periodontal tissues.

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059:

K. AUNG, K. AKIYAMA, T. KUBOKI
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry andPharmaceutical Sciences, Okayama, Japan

Joint Symposium

Objectives: Mesenchymal stem cells (MSCs) play important roles in the repair of damaged tissues and immunotolerance.
On the other hand, aging is known to impair MSCs function. However, how functionally impaired MSCs affect the local
inflammatory condition and tissue deterioration is still not known. The purpose of this study is to clarify the relationshipbetween MSC’s function and local tissue destruction in disease conditions using a ligature-induced mouse periodontitis model.
Methods: Characterization of MSCs in vitro were performed by CFU-f, scratch, cell-surface antigen expression, senescence, celldifferentiation, and immunomodulation assays. A periodontitis model was developed in 5- (young) and 50-week-old (aged)
C57BL/6J mice, by ligating mandibular first molars with silk threads. Mice were sacrificed at 0, 3, and 10 days after ligation forfurther experiment. Alveolar bone destruction was evaluated by m-CT, HE, and IHC analysis.Results: In vitro characterization
data showed that MSCs markers (Sca-1, CD90, CD146), colony formation, migration, and osteogenic differentiation of aged
MSCs was significantly declined than young MSCs. Moreover, senescence-associated β galactosidase activity was significantly
higher in aged MSCs. Importantly, aged MSCs presented a decreased expression of FAS-L, which was associated with a lower
immunomodulatory property of aged MSCs to induce T cell apoptosis compared with young MSCs. The m-CT and histologicalanalysis showed a more severe bone loss associated with increased osteoclast activity in aged mice than young mice. In aged
mice, the accumulation of inflammatory T and B cells was higher, whereas the percentage of PDGFRα+ MSCs, was significantly
lower than in young mice.Conclusions: This is the first study showing that aging-induced impairment of MSC function, including
immunomodulatory response, is potentially correlated with progressive periodontal tissue deterioration. Finding out themechanism of aging in MSCs improve the effectiveness of MSCs therapy and tissue destruction in the elderly patient.

Special Lectures

Relationship between age-related changes in MSC functions and periodontal tissues destruction inligature induced periodontitis mouse model

Symposium I

MSC conditioned medium from older mice affects its osteogenic differentiation capacity
J. ZHANG, K. AKIYAMA, K. MAEKAWA, T. KUBOKI
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry andPharmaceutical Sciences, Okayama, Japan

Poster Presentation

─ 95 ─

Rising Scientist Session

Objectives: Mesenchymal stem cells (MSCs) have self-renewal and multipotent differentiating abilities, therefore many studies
have been conducted in the field of tissue regeneration. It is known that MSC functions are affected by various factors such as
host aging, but why this functional impairment occurs and the detailed mechanism is still not clear until now. To uncover this,
we investigated osteoblastic differentiation capacity of conditioned medium of MSCs from different ages of mice.Methods:
The bone marrow derived MSCs from different aged (5-week-old and 50-week-old), female C57BL / 6J mice were cultured
as previously described (Aung et al., 2020). The conditioned medium was obtained by centrifuging culture supernatant fromdifferent aged mice MSCs (Amicon Ultra filter Unit). Passage 2 cells were used for differentiation assay with osteogenic medium
containing with 0, 10, 30% of the conditioned medium for 14 days. The osteogenic gene expression levels were evaluated byreal time RT-PCR for alkaline phosphatase (Alp) and osteocalcin (Ocn).Results: Alp expression level was significantly decreasedin 5-week MSCs group with 30% conditioned medium from 50-week-old mice MSCs. Ocn expression was also decreased in
5-week-old MSCs with 30% conditioned medium from 50-week mice MSCs but not significantly. Meanwhile, expression levelsof both Alp and Ocn were not changed in 50-week-old MSCs with any concentration of conditioned medium from 5-week-old
mice MSCs.Conclusions: These results indicated that MSCs from older hosts may produce soluble factors to inhibit osteogenic
differentiation of MSCs, while it might be difficult to recover the osteogenic differentiation capacity of aged mice MSCs even if
cultured with soluble factors from younger MSCs. Identifying these inhibition factors for osteogenic differentiation might leadto a new strategy to recover the function of aged MSCs by blocking their signals.

Symposium II

060:

061:

Extracellular vesicles-mediated novel therapeutic mechanism of deciduous toot pulp stem cell-based
therapy for systemic lupus erythematosus
S. SONODA, T. YAMAZA
Department of Molecular Cell Biology and Oral Anatomy, Division of Oral Biological Sciences, Kyushu University Graduate School of
Dental Science, Fukuoka, Japan

Objectives: Therapeutic efficacy of single systemic transplantation of stem cells from human exfoliated deciduous teeth (SHED)
for systemic lupus erythematosus (SLE) is reported. However, the mechanisms underlying the SHED-based therapy in SLE
remain unclear. In this study, we examined therapeutic function of SHED-releasing extracellular vesicles (SHED-EVs) on SLE-like
disorders in MRL/lpr mice.Methods: Secretion of EVs from SHED were suppressed by RAB27A knockdown via siRNA transfection(RAB27A knocked-down-SHED). Then, RAB27A knocked-down-SHED and non-treated SHED were systemically transplanted into
MRL/lpr mice. SHED-EVs were isolated from the culture supernatant of SHED. Then SHED-EVs were systemically administrated
to MRL/lpr mice with or without RNase pre-treatment. Recipient bone marrow mesenchymal stem cells (BMMSCs) wereisolated from SHED-EVs-administrated MRL/lpr mice. Then recipient BMMSCs were systemically transplanted into MRL/lpr
mice.Results: While non-treated SHED ameriorated SLE-like disorders in MRL/lpr mice, RAB27A knocked-down-SHED showed
reduced therapeutic efficacy. Systemic administration of SHED-EVs ameliorated SLE-like disorders in MRL/lpr mice via improving
activity of hematopoietic niche formation and immunoregulation of recipient BMMSCs by rescuing Tert associated telomerase
activity. The secondary transplantation of recipient BMMSCs ameliorated SLE-like symptoms of MRL/lpr mice. RNase treatment
depleted RNAs within SHED-EVs attenuated the therapeutic benefit in MRL/lpr.Conclusions: These results indicated that RNAs
within SHED-EVs ameliorated SLE-like disorders in MRL/lpr mice by improving telomerase activity-mediated recipient BMMSCs
functions. The present study reveals the novel mechanism of SHED-based therapy.

─ 96 ─

Author Index-演者索引
A

FURUSAWA, M., 050

IWASHITA, M., 035, 037

ABIKO, Y., 025, 052, 054

FURUTA, M., 027

IWATA, K., 034

ADACHI, T., 044

FUSEJIMA, F., 009, 047

K

AIBA, S., 039

G

KADOTA, Y., 047

AIDA, N., 050

GOTO, K., 004, 007

KAGEYAMA, S., 027

AKASAKA, T., 016

H

KAJIWARA, K., 043

AKIYAMA, K., 059, 060

HAMANO, A., 010

KAMIJO, R., 057

AKIYAMA, Y., 028

HAMANO, S., 058

KAMIOKA, H., 023

ALHASAN, A., 010

HARA, E., 022, 023

KANAMURA, N., 031, 044

ALTANKHISHIG, B., 030

HARADA, F., 052

KANEKO, H., 058

ANADA, R., 023

HASEGAWA, D., 058

KARNTIANG, P., 012

ARIMA, E., 047

HASEGAWA, T., 016

KARTIKASARI, N., 014

ARIWANSA, D., 052

HAYASHI, C., 055, 056

KATAOKA, K., 020

ARQUB, S. A., 033

HAYASHI, H., 016

KAWAKAMI, K., 055, 056

ASADA, Y., 024

HAYASHI, K., SI-1

KAWARABAYASHI, K., 034

ASAHI, Y., 003

HAYASHI, M., 003, 051

KHATUN, M., 030

ASAKAWA, M., 027

HAYASHI, M., 035

KHURELCHULUUN, A., 052

ASAUMI, H., 006

HIGA, K., 028

KINOSHITA, K., 046

AUNG, K., 059

HIRAKI, D., 052

KISHIDA, T., 031

AYUKAWA, Y., 011, 046

HIRATA-TSUCHIYA, S., 039

KITAGAWA, T., 054

AZUMA, T., 029

HOTTA, S., 047

KITAMURA, T., 002

AZUMA, T., 050

HUANG, H., 051

KIYONO, H., SLBI

KLANLIANG, K., 003

BABA, Y., 042

IGARASHI-TAKEUCHI, H., 041

KOBARA, M., 044

D

IKEDA, H., 012, 032

KOMADA, W., 017, 048, 049

DEWAKE, N., 036

IMAGAWA, M., 037

KOMICHI, S., 051

E

INADA, E., SII-3

KOMINE, K., 015

EBISU, S., 003

INADA, J., 028

KONDO, D., 049

EGUSA, H., 014

INAGAKI, M., 038

KOUNO, M., 028

ESHITA, Y., 036

IOHARA, K., SII-2

KOYANAGI, K., 020

F

IPPOSHI, K., 010

KOYANO, K., 046

FUJINAKA, H., 036

ISHIKAWA, K., 011

KUBOKI, T., 059, 060

FUKUDA, T., SII-4

ITOYAMA, T., 010, 058

KUO, C., 033

FUKUDA, T., 035, 055

IWAI, K., 029

KURAJI, R., RS-2

FUKUMOTO, S., SII-1

IWAMOTO, T., 034

KURAMITSU, Y., 054

FUKUMOTO, S., 034

IWASHITA, M., RS-4

KURIKI, N., 003

─ 97 ─

KURIYAMA, Y., 019

NAGAYASU, H., 052

OTSUGU, M., 001

KWON, K., JS-4, KL

NAKA, S., 004, 005, 040

OZAKI, T., 017

L

NAKANISHI, K., 016

P

LAGRAVERE, M., 033

NAKANO, K., 001, 002

PAUDEL, D., 025, 052, 054

M

NAKAO, Y., 055, 056

R

MA, J., 027

NAKATA, T., 044

RAJU, R., 026, 053

MA, X., 036

NAKATSU, S., 036

RAMAN, S., 026, 053

MACHI, H., 003

NAKAZATO, H., 050

REYNOLDS, E., G-1

MAEDA, H., 010, 058

NEMOTO, E., 039

S

MAEKAWA, K., 060

NEMOTO, R., 048

SAITO, M., 039

MAEZONO, H., 003

NESSA, N., 044

SAITO, M.M., 018

MANOME, Y., 045

NISHIMURA, F., 035, 037, 055, 056

SAITO, T., 030

MATAYOSHI, S., 001, 002

NISHIMURA, Y., 035, 037

SAKAGUCHI, Y., 009

MATSUKA, Y., 026, 053

NOGUCHI, H., SII-3

SANO, T., 035

MATSUMOTO, S., 051

NOMURA, R., 001, 002

SANUI, T., 035, 055, 056

MATSUMOTO, T., 022, 023

NOMURA, T., 028

SAITO, M., JS-3

MATSUMOTO-NAKANO, M.,

NOZAKI, K., 015, 017, 048, 049

SAITOH, I., SII-3

NUMABE, Y., 041, 045

SATO, J., 025

MATSUMURA, M., 015

O

SATO, K., 036

MATSUMURA, M., 015

OGAWA, T., 008

SATO, K., 037

MATSUO, M., 013

OGINO, Y., 011, 046

SATO, M., SII-3

MATSUOKA, D., 004

OGUMA, H., 016

SATO, S., 038

MAZDA, O., 031

OHARA, M., 019

SATO, T., 021

MEHTA, S., 033

OHATA, J., 002

SATOU, J., 052

MINABE, M., 028

OISHI, S., 048, 049

SAWA, Y., 043

MIURA, H., 015, 017, 048, 049

OKADA, M., 022, 023

SAWASE, T., SI-2

MIYAI, Y., 040

OKAMOTO, M., 051

SHIBA, H., 039

MIYAKAWA, Y., 024

OKAMURA, S., 019

SHIMAOKA, T., 003

MIYAKE, T., 020

OKI, K., 046

SHIMIZU, H., 012, 032

MIYAMOTO, E., 019

OKURA, K., 053

SHIMIZU, Y., 019

MORIKAWA, T., 025, 052, 054

OMNIA, S., 015

SHIN, C., 015

MORIYAMA, K., 008

OMORI, K., RS-3

SHINJO, T., 035, 037, 055, 056

MORIYAMA, K., 051

OMORI, S., 048, 049

SHINOMIYA, K., 057

MURAKAMI, S., SI-4

ONISHI, A., 052

SOE, K.M., 008

N

ONO, T., 010

SONODA, S., 061

NAGAMATSU, Y., 012, 032

ONODERA, S., 050

SUEHIRO, Y., 001, 002

NAGAMOTO, K., 016

OSHIMA, M., 026, 053

SUGII, H., 010, 058

NAGAOKA, N., 023

OTAKE, S., 017

SUGIMOTO, A., 034

004, 005, 006, 007, 040

─ 98 ─

SUMA, S., 027

YAMADA, S., 039

YUN, Jeong-Ho, JS-2

SUZUKI, O., SI-3

YAMAKI, K., 021

Z

SUZUKI, S., RS-1

YAMAKOSHI, Y., 018

ZEZE, T., 035, 037

SUZUKI, S., 039

YAMAKOSHI, Y., 024

ZHANG, J., 060

T

YAMAMOTO, K., 031

TABATA, K., 005

YAMAMOTO, T., 031

TACHIBANA, T., 045

YAMAMOTO, T., 044

TACHIKAWA, T., 028

YAMANAKA, K., 009

TADINADA, A., 033

YAMASAKI, K., 039

TAKAHASHI, K., 042

YAMASHITA, A., 035, 037

TAKAHASHI, S., 028

YAMASHITA, K., 010

TAKAHASHI, S., 052

YAMASHITA, Y., 027

TAKAHASHI, Y., 051

YAMATO, H., 055, 056

TAKESHITA, T., 027

YAMAZA, T., 061

TAKEUCHI-IGARASHI, H., 045

YAMAZAKI, K., 042

TAMAHARA, T., 021

YAMAZAKI, K., 041

TAMAOKI, S., 043

YAMAZAKI, M., 042

TANAKA, K., 011

YAN , H., 053

TERUI, H., 039

YANAGISAWA, S., 020

TO, M., 013

YANAGISAWA, T., 038

TOMOFUJI, T., 029

YANAKA, W., 015

TOMOKIYO, A., 010, 058

YANO, Y., 036

TOYODA, M., 055, 056

YASUDA, N., 019

TSUCHIYA, A., 010, 011

YASUHIRO, M., 030

TSUKAHARA, R., 048, 049

YOKOSUKA, Y., 017, 049

TSUKIYAMA, Y., 046

YONENAGA, T., 029

U

YOSHIDA, A., 036

UEHARA, O., 025, 052, 054

YOSHIDA, A., 038

UPADHYAY, M., 033

YOSHIDA, K., 025, 052, 054

W

YOSHIDA, S., 010, 058

WASHIO, J., 021

YOSHIDA, Y., 016

WASKITHO, A., 026, 053

YOSHIMATSU, S., 048, 049

WATANABE, M., 051

YOSHIMURA, K., 036

WATANABE, Y., 055, 056

YOSHINARI, N., 036

Y

YOSHIZAKI, K., JS-1YADAV, S., 033

YOSHIZAKI, K., 034

YAMADA, A., 057

YOTSUMOTO, K., 055, 056

YAMADA, M., 014

YUAN, H., 039

─ 99 ─

謝

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科研製薬 株式会社（50 音順）─ 101 ─

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The 69th Annual Meeting of Japanese Association for Dental Research
Program and Abstracts of Papers

Copyright © 2021 by the 69th Annual Meeting of Japanese Association for Dental
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Section of Periodontology, Faculty of Dental Sciences, Kyushu University, Fukuoka, Japan
Maidashi 3-1-1, Higashi-ku, Fukuoka city, Fukuoka, 812-8582, JAPANE-mail: E-mail : jadr69@dent.kyushu-u.ac.jp

第回国際歯科研究学会日本部会総会・学術大会

69

The 69th Annual Meeting ofJapanese Association for Dental Research

第 69 回 国際歯科研究学会

日本部会総会・学術大会
Re-defining the Mission of Dental
Research towards Post-corona WorldPHOTO : Fumio Hashimoto

October 24 (Sun.) – October 25 (Mon.), 2021
Hybrid Meeting（Onsite at Kyushu University and Online）「写真提供：福岡市」