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method for measuring lactate uptake by hepatocytes in culture using free fatty acids as a substrate. A new method for measuringlactate uptake by hepatocytes in primary culture is described. The technique utilizes the metabolism of free fatty acids releasedby the hepatocytes as a substrate in order to measure lactate production. Free fatty acids present in the medium are taken up by
the cells and the excess is oxidized by the intracellular enzymes. Lactate produced by the cells is oxidized within themitochondria and NAD+ is reduced to NADH which subsequently undergoes reduction by diaphorase of the hexokinase cycle
to measure the uptake of lactate. The method is simple, very sensitive, does not interfere with cell metabolism and results of up
to 900 nmol lactate/mg protein per hour have been obtained. The method appears to be applicable to cell cultures from a varietyof tissues. The tissue response to different substrates is shown. top_signal_7_nopitch_log.mzML false
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