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Cholesterol esterase (COE-311)
First issue : May. 1, 2014
Revised: May. 31. 2022
SDS No.1004F

Safety Data Sheet
1 Chemical Product and Company Identification
Identification of the productProduct CodeSupplier
NameAddress
Department
Phone
Fax
Recommended use and restrictions on use

Cholesterol esterase
COE-311TOYOBO CO.,LTD.
Osaka Umeda Twin Towers South,
1-13-1 Umeda Kita-ku, Osaka 530-0001, Japan
Biotechnology Overseas Sales and Marketing Department+81-6-6348-3846+81-6-6348-3833
diagnostic product2 Hazard Identification
Most Important Hazards and EffectsHazards and Effect for human health
GHS classification
Physical hazards
Health hazards
Acute toxicity : Oral
Acute toxicity : Dermal
Acute toxicity : Inhalation
Acute toxicity : Inhalation
Skin corrosion/irritation
Serious eye damage/Eye irritation
Sensitization : Respiratory
Sensitization : Skin
Germ cell mutagenicity
Carcinogenicity
Toxic to reproduction
Specific target organ toxicity(Single)Specific target organ toxicity(RAspirationdhazard)
Environmental hazards
Acute hazards to the aquaticLong-term hazards to the aquaticHazard to the ozone layer

Harmful if Boric acid is swallowed.
Lethal dose (Boric acid) : Adult 10g, Child 5g
Category 5
Not applicable
Category 2
Category 2
Category 1B
Category 1(nervous system,digestive trace)
Category 3(respiratory tract)
Category 3
Category 3-:Classification not possible

GHS Label Elements
Symbol/Pictograms

Signal word
Hazard statements

DangerHarmful if swallowed.
Cause skin irritation.
Causes serious eye irritation.
Damage fertility or the unborn childCauses damage to organs (Nervous system, digestiveorgan, respiratory organ).
May cause respiratory irritation.

2/5Cholesterol esterase (COE-311)
First issue : May. 1, 2014
Revised: May. 31. 2022
SDS No.1004F

Safety Data Sheet
Harmful to aquatic life with long lasting effects.
Instructions
Precaution Obtain special instructions before use.
Do not handle until all safety precautions have beenread and understood.
Use only outdoors or in a well-ventilated area.
Wear protective gloves/protective clothing/eye
protection/face protection.
Do not breathe dust/fume.
Do not eat, drink or smoke when using this product.
Wash hands thoroughly after handling.
Avoid release to the environment.
First-aid measures IF INHALED: Remove person to fresh air and keep
comfortable for breathing.
If on skin:Take off immediately all contaminatedclothing. Rinse skin with water /shower.

Important symptoms (Boric acid)3

If skin irritation occurs: Get medical
i cautiously with water for several
Ifd iin /eye:Rinse
minutes. Remove contact lenses, if present and easy todo. Continue rinsing.
If eye irritation persists:Get medical advice
IF exposed or concerned: Get medical attention/advice.
Get medical advice/attentions if you feel unwell.
Storage Store locked up.
Store in a well-ventilated place. Keep container
tightly closed.
Disposal Dispose of contents/container in accordance withlocal/regional/national/international/regulation.
Symptoms of vomit,diarrhea,collapse,erythema may appear
after 2-3 hours later.

Composition/Information on Ingredients
Substance/Mixture
Chemical Nature
Chemical specificity
Amounts contained
Cholesterol esterase
Boric acid
Ingredients Contributing to the Hazard
Common Chemical nameAmounts contained
Chemical formulaCAS#
Common Chemical nameConcentration or concentration range
Chemical formulaCAS#4Mixture
Cholesterol esterase
Freeze and drying powder including enzyme
Approximate 50%(W/W)
CAS#9026-00-0
10043-35-3
Boric acidca 10%(W/W)
H3BO310043-35-3
Polyethylene Glycol-p-octylphenyl Ether
TritonX-100
ca 6% (W/W)
C8H17-C6H4O(C2H4O)nH
9002-93-1

First Aid Measures
Inhalation
Skin Contact

Remove to fresh air. Consult a physician whenunpleasantness occurs.
Wash off with plenty of water.

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Cholesterol esterase (COE-311)
First issue : May. 1, 2014
Revised: May. 31. 2022
SDS No.1004F

Safety Data Sheet
Eye Contact
Ingestion
Most important symptoms/effects, acuteand delayed.

Rinse off with running water for several minutes.
Consult a physician afterwards.
Rinse mouth. Swill plentiful amount of water or milkfor immediate vomiting. Consult a physician.
Inhalation: Cough, Sore throat
Skin: Redness
Eyes: Redness, Pain
Ingestion: Abdominal pain. Convulsions. Diarrhoea.
Nausea. Vomiting. Skin rash.

5 Fire Fighting Measures
Extinguishing Media
Particular hazardous

Water spray,dry chemical powder,or carbon dioxide etc.
Firefighter should work from the windward side.

6 Accidental Release Measures
Personal Precautions, protective
equipment, and emergency procedure
Environmental Precautions
Methods and materials for containment andcleaning up

Wear proper protective gear to avoid eye/skin contact
and inhalation. Work from the windward side.
Do not wash away into sewer, watercourse or river.
Take up under vacuum using dust collecting filter, wash
residual spill with copious amounts of water. Usecloth, paper or anything similar to soak up thesolution leaking out of the container.(Waste water
should be treated with activated sludge or adsorbedwith activated carbon etc.)

7 Handling and Storage
Handling
Technical Measures
PrecautionsStorage
Technical Measures
Incompatible substances and mixtures
Storage Conditions
Packaging Materials

Wear protective gear to avoid eye/skin contact andinhalation.
Do not drop the container to prevent the content
popping out.
Keep sealed container in freezer.
None specified.
Store under -20℃ to avoid deactivating.
Use the initial container of the product.

8 Exposure Controls/Personal Protection
Engineering controls
Occupational exposure limit,biologicallimit
Personal Protective Equipment
Respiratory Protection
Hand Protection
Eye Protection
Skin and Body Protection

Provide shower and eye washing apparatus nearby.
(Boric acid) TLV: TWA 2mg/m3, STEL 6mg/m3

Protecting mask
Protecting gloves
Safety goggles
Long sleeve working wear

9 Physical and Chemical Properties
Physical State, form and colour
Odour
pH
Flash Point
Explosiveness
Density

Powder / Light brownNo odourApproximate pH7.5 (1% W/V)
No information available.
No information available.
No information available.

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Cholesterol esterase (COE-311)
First issue : May. 1, 2014
Revised: May. 31. 2022
SDS No.1004F

Safety Data Sheet
Solubility

Freely soluble in water.

10 Stability and Reactivity
Stability
Possible hazard
reactions at specific condition
Conditions to avoid
Materials to avoid
Hazardous Decomposition Products

Stable at temperatures below -20℃.When left for longat room temperature,proteins might be degraded, which
does not cause any hazardous reaction.
Prolonged storage under higher temperature than room
temperature and high humidity.
Oxidizing agent
No information available.

11 Toxicological Information
No information available on the product in toxicity, however, the information on the boric acidand triton X-100 is shown below.
Acute Toxicity (Oral)
Skin corrosion/irritation
Serious eye damage/irritation
Germ cell mutagenicityCarcinogenicity
Toxic to reproduction

Boric acid(Rat)
LD50 2660mg/kg
Guinea pig skin irritation tests (exposure duration
unknown) : At 24 and 72 hours, moderate irritation
Irritates the human eye (though the severity of theeffects and recovery period are not presented).
in vivo: Mouse bone marrow cell mutagenicity tests(micronucleus tests): Negativein vitro: Reverse mutation test using bacteria, genemutation test and chromosome aberration test using
mammalian cultured cells: Negative
ACGIH classified as Category A4 (as inorganic boratecompounds)
Mouse:Reproductive Assessment by Continuous Breeding,Rat:3 generation reproductive toxicity study: Adverse
effects on reproduction
Rat:Developmental toxicity:Teratogenicity is observed.

Specific target organ systemic toxicity Human: Nausea, vomiting, abdominal pain and diarrhea,
central nervious system depression, comvulsion and(Single exposure)respiratory irritation.
Animal studies: Slight respiratory irritation wasobserved.
Specific target organ systemic toxicity Human: No information available. Animal studies:Oraladministration, adverse effects were observed at over(Repeated exposure)the guidance dose for category 2.
Though this corresponds to "not classified" in oralroute, no information of the effects via the otherroutes of administration.

Eye damage/irritation

TritonX-100
Rabbit eye irritation was observed, and the symptoms
had been healed within 21 days （ECETOC TR 48（2）（））

12 Ecological Information
No information available on the product in toxicity, however, the information on the boric acidand triton X-100 is shown below.
Boric acid

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Cholesterol esterase (COE-311)
First issue : May. 1, 2014
Revised: May. 31. 2022
SDS No.1004F

Safety Data Sheet
EcotoxicityBioaccumulative potential
Ecotoxicity
Persistence /Degradability

Algae (Pseudokirchneriella subcapitata) 72hr ErC50 =290 mg/L
Crustacea (Daphnia magna) 48hr LC50 = 133 mg/L (asboron)
Fish (Coho salmon) 96hr LC50 = 447 mg/L
Fih (R i bnottbioaccumulativet) 87d
NOEC
2 1/L
Considered
Triton X-100
LC50/96hr = 3mg/L (bluegill)
Persistent substance: 22% by BOD (National Institute ofTechnology and Evaluation, Japan)
Enzyme and other organic ingredients are biodegradable
and does not remain on the environment for long.

13 Disposal Considerations
ResiduesA pollution container and packing

Dispose of in accordance with all applicable local andnational laws and regulations.
Wash with copious amounts of water and waste conforming
to local regulations depending on the type of thematerial.

14 Transport Information
International regulations
Domestic regulations
Specific precautions transport measuresand conditions

Not applicableNot applicable
Avoid direct sunshine and check the container andloading to prevent leakage or turnover,fall and damage.
Do not load with foods and feed. Keep at temperatures
below -20℃.

15 Regulatory Information
Registration, Evaluation, Authorizationand Restriction of Chemicals (EU)Common Chemical nameCommon Chemical nameRegulations

The following ingredient is included in SVHC (Candidate
list of authorization).
Boric acid
Polyethylene Glycol-p-octylphenyl Ether (Triton X-100)
Follow all of laws and regulations in your country.

16 Other Information
Notice

The information shall not be taken as being all
inclusive and is to be used only a guide.All materials
and mixtures may be present unknown hazards and should
be used with caution.The SDS is subject to revision as
new information becomes available.The information inthis SDS, to the best of our knowledge, is accurate andcorrect. However,TOYOBO makes no warranty and assumes
no liability whatsoever in connection with any use ofthis information.