᎘Ẽᛶ⳦◊✲ศ㔝
Division of Anaerobe Researchࠛ501-1193 ᒱ㜧ᕷᰗᡞ 1 ␒㸯E-mail : kenki@gifu-u.ac.jp
TEL : 058-230-6554, 6555
FAX : 058-230-6551┠࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭‫ ی‬᎘Ẽᛶ⳦◊✲ศ㔝
1. ἢ㠉

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2. ⫋ဨᵓᡂ

࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭

࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭

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3. ◊✲࡜άືࡢ᪉㔪ࠕ ࡘࡢᰕࠖ4. ศ㔝᪋タ࡜୺࡞タഛ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭

࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭

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5. ᎘Ẽᛶ⳦ಖᏑ⳦ᰴࡢಖ᭷≧ἣ

6. ศ㔝ࡢᴗົ࡜ᨭ᥼

7. ᨭ᥼ࡢᐇ⦼

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8. ᩍဨࡢ◊✲㺃ᩍ⫱㺃♫఍άື࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭‫ ی‬᎘Ẽᛶ⳦◊✲ศ㔝᎘Ẽᛶ⳦◊✲ศ㔝ࡣ୺࡟ࣄࢺ࣭ື≀࡟⏤᮶ࡍࡿ೫ᛶ᎘Ẽᛶ⳦㸦㓟⣲Ꮡᅾࡀ⏕Ꮡ࡟୙฼࡟ാࡃ⣽⳦㸧ࢆᖜᗈࡃᢅࡗ࡚࠸ࡿᅜෆ࡛၏୍ࡢ◊✲᪋タ࡛࠶ࡿࠋጞࡲࡾࡣࠊᡃࡀᅜ࡟࠾ࡅࡿ⮫ᗋ᎘Ẽᛶ⳦◊✲ࡢࣃ࢖࢜ࢽ࢔࡛࠶ࡿᨾ㕥ᮌ㷋୍㑻ࠊᨾୖ㔝୍᝴ࡢ୧ᒱ㜧኱Ꮫྡ㄃ᩍᤵࡢᴗ⦼࡟ᑐࡋ࡚ 1978 ᖺ㸦᫛࿴ 53 ᖺ㸧࡟タ⨨ࡉࢀࡓ་Ꮫ㒊㝃ᒓ᎘Ẽᛶ⳦ᐇ㦂᪋タ࡛࠶ࡿࠋ௨᮶ࠊᒱ㜧኱Ꮫࡢ≉Ⰽ࠶ࡿ᪋タ࡜ࡋ࡚ࠊᡃࡀᅜ࡟࠾ࡅࡿ⮫ᗋ᎘Ẽᛶ⳦ᏛࡢⓎᒎ࡟ᐤ୚ࡋ࡚ࡁࡓࠋ⌧ᅾ࡛ࡣࠊ⛉Ꮫ◊✲ᇶ┙ࢭࣥࢱ࣮ࡢ㸯ศ㔝࡜ࡋ࡚ࠊ⮫ᗋ⣽⳦ࡢࡳ࡟࠿࠿ࢃࡽࡎࠊ᎘Ẽⓗ⎔ቃ࡛ࡢᐇ㦂࡟ࡘ࠸࡚ࡢࢥࣥࢧࣝࢸ࣮ࢩࣙࣥࠊᶵჾࡢ౪୚ࠊ೫ᛶ᎘Ẽᛶ⳦ࡢศㆡ➼ࡢᨭ᥼ࢆ⾜ࡗ࡚࠸ࡿࠋ᪋タࡢᡂࡾ❧ࡕ࡜ᛶ㉁ୖࠊ௚ศ㔝࡜␗࡞ࡾࠊᏛෆඹྠ฼⏝᪋タ࡛ࡣ↓ࡃࠊឤᰁ⑕㡿ᇦ࡜ࣛ࢖ࣇࢧ࢖࢚ࣥࢫ◊✲࡟㛵ࢃࡿ᎘Ẽᛶ⳦ࡢࣞࣇ࢓ࣞࣥࢫࢭࣥࢱ࣮ᶵ⬟ࢆᣢࡘ᪋タ࡜ࡋ࡚ࠊ඲
ᅜⓗ࡞ᨭ᥼ࢆ⾜ࡗ࡚࠸ࡿࠋ᎘Ẽᛶ⳦ศ㞳ቑ⳦⏝ᇵᆅ
GAMᇵᆅ䠄Gifu Anaerobic Medium䠅 䛸)XVREDFWHULXPᒓ䛾㚷ูᇵ㣴䛾䛯䜑䛾ᒱ኱ᘧ᎘Ẽᇵ㣴䝆䝱䞊䠄㯪᫂ᮇ䛻㛤Ⓨ䛥䜜䛯᎘Ẽ䝆䝱䞊1960ࠥ70㡭䠛䠅ኚἲFMᇵᆅ

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᫛࿴53ᖺ10᭶་Ꮫ㒊㝃ᒓ᎘Ẽᛶ⳦ᐇ㦂᪋タ㛤タࡉࢀࡿ᪋タ㛗㕥ᮌ⚈୍㑻㸦ే௵㸧ࠊᑓ௵ᩍᤵ ୖ㔝୍᝴

᫛࿴54ᖺ4᭶᪋タ㛗ୖ㔝୍᝴ ╔௵

ᖹᡂ5ᖺ 3᭶᪋タ㛗ୖ㔝୍᝴ ㏥௵

ᖹᡂ5ᖺ 4᭶᪋タ㛗Ώ㑓㑥཭ ╔௵

ᖹᡂ15ᖺ4᭶ᨵ⤌࡟ࡼࡾ⏕࿨⛉Ꮫ⥲ྜᐇ㦂ࢭࣥࢱ࣮᎘Ẽᛶ⳦ᐇ㦂ศ㔝ศ㔝㛗 Ώ㑓㑥཭

ᖹᡂ17ᖺ4᭶ᨵ⛠࡟ࡼࡾ⏕࿨⛉Ꮫ⥲ྜ◊✲ᨭ᥼ࢭࣥࢱ࣮᎘Ẽᛶ⳦◊✲ศ㔝ศ㔝㛗 Ώ㑓㑥཭㸦ᖹᡂ25ᖺ3᭶ࡲ࡛㸧⏣୰㤶࠾㔛㸦ᖹᡂ25ᖺ4᭶㹼㸧ᖹᡂ30ᖺ4᭶ᨵ⤌࣭ᨵ⛠࡟ࡼࡾ◊✲᥎㐍㺃♫఍㐃ᦠᶵᵓ ⛉Ꮫ◊✲ᇶ┙ࢭࣥࢱ࣮᎘Ẽᛶ⳦◊✲ศ㔝ศ㔝㛗 ⏣୰㤶࠾㔛௧࿴2ᖺ4᭶ᨵ⤌࣭ᨵ⛠࡟ࡼࡾ㧗➼◊✲㝔 ⛉Ꮫ◊✲ᇶ┙ࢭࣥࢱ࣮᎘Ẽᛶ⳦◊✲ศ㔝ศ㔝㛗 ⏣୰㤶࠾㔛

2. ⫋ဨᵓᡂᩍᤵ㸸⏣୰㤶࠾㔛ᢏ⾡⿵బဨ㸸୰ᕝᮁᏊ

3. ◊✲࡜άືࡢ᪉㔪ຓᩍ㸸ᚋ⸨㝯ḟᯘᑗ኱ᇼ஭⨾⣖ࠕ4ࡘࡢᰕࠖ

1㸧᎘Ẽᛶ⳦ឤᰁ⑕࡟ࡘ࠸࡚ࡢᇶ♏ⓗ⮫ᗋ⣽⳦Ꮫⓗ࡞◊✲ࢆ⾜࠺ࠋࡇࡢ◊✲ᡂᯝ࡟ࡼࡾࠊࡇࢀࡲ࡛ࡶ⮫ᗋ᎘Ẽᛶ⳦ᏛࡢⓎᒎ࡟㈉⊩ࡋ࡚ࡁࡓࠋศ㞳㺃ᇵ㣴㺃ྠᐃࢶ࣮ࣝࡢⓎᒎ࡟ࡼࡾࠊ⮫ᗋ᎘Ẽᛶ⳦ࡢ␿Ꮫࡶ㐣Ώᮇ࡟࠶ࡾࠊࡲࡓࠊᚤ⏕≀ࡣࠊ♫఍᝟ໃࠊ⏕ά⩦័➼࡛ࡶࡓࡽࡉࢀࡿ⎔ቃኚ໬࡟ࡼࡾኚ໬ࡋ࠺ࡿࡓࡵࠊ᝟ሗࡢUp Dateࡀᚲせ࡛࠶ࡿࠋ␿Ꮫ᝟ሗࡢ඘ᐇࡀࠊṇࡋ࠸デ᩿ἲ࡟ᇶ࡙ࡃ㐺ษ࡞἞⒪ἲࡢ㑅ᢥࢆྍ⬟࡟ࡋࠊ἞⒪ἲࡢᨵⰋࡸ᪂ࡋ࠸἞⒪ἲࡢ㛤Ⓨ࡟⧅ࡀࡿ࡜࡜ࡶ࡟ࠊண㜵་Ꮫ࡟ࡶ⧅ࡀࡿࠋ

2㸧◊✲ᡂᯝࡢᛂ⏝ࠊ᎘Ẽᛶ⳦ឤᰁ⑕ࡢデ᩿ἲࡢᇶ‽໬ࡸ࣐ࢽࣗ࢔ࣝ໬ࠊࡑࡋ࡚ࡑࡢᩍ⫱ᬑཬࢆ⾜࠺ࠋᙜ᪋タ࡛ẖᖺ㸯ᅇ1㐌㛫ࡢ᪥⛬࡛⾜࠺᎘Ẽᛶ⳦᳨ᰝᢏ⾡ኟᮇࢭ࣑ࢼ࣮ࡣࠊࡍ࡛࡟40ᖺ௨ୖࡢṔྐࡀ࠶ࡾࠊ᎘Ẽᛶ⳦ឤᰁ⑕デ᩿ࡢࡓࡵࡢᢏ⾡ᣦᑟ࡜᝟ሗᥦ౪ࡢሙ࡛࠶ࡾ⥆ࡅ࡚࠸ࡿࠋࡉࡽ࡟ࠊཷㅮ⪅࡟ᑐࡍࡿㅮ⩦఍⤊஢ᚋࡢᨭ᥼άືࡣࠊ㟁ヰࡸe-mail࡛ࡢࢥࣥࢧࣝࢸ࣮ࢩࣙࣥࠊ᳨య࠿ࡽࡢ⑓ཎ⳦ࡢ⣽⳦Ꮫⓗ᳨ᰝࢧ࣏࣮ࢺࡢᙧ࡛ᐇ᪋ࡋ࡚࠸ࡿࠋࡲࡓࠊ◊✲ᡂᯝࢆᑟධࡋࡓࠊ⳦ឤᰁ⑕ࡢデ᩿ἲࡢᶆ‽໬ࠊ࣐ࢽࣗ࢔ࣝ໬࡟ດࡵࡿࠋ3㸧⮫ᗋ⌧ሙ࠿ࡽࡢ᎘Ẽᛶ⑓ཎ⳦ࡢ཰㞟࡜ಖᏑ࠾ࡼࡧࡑࡢศ୚ࢆ⾜࠺ࠋࡑࡢᛶ㉁ୖࠊᝈ⪅࠿ࡽࡢ᎘Ẽᛶ⑓ཎ⳦ࡢ཰㞟ࡣ㏻ᛶ⳦࡟ẚ࡭࡚ࠊᴟࡵ࡚㞴ࡋ࠸௙஦࡛࠶ࡿࠋᙜศ㔝࡛ࡣࠊ᎘Ẽᛶ⳦ࡢཧ⪃⳦ᰴࡸ඲ᅜྛᆅࡢ⑓㝔࠿ࡽࡢデ᩿ᨭ᥼౫㢗ࢆ㏻ࡋ࡚ศ㞳ࠊྠᐃࡋࡓ⳦ᰴࢆಖᏑࡋ࡚࠸ࡿࠋ
⌧ᅾࡢ࡜ࡇࢁࠊಖᏑᰴᩘࡣ7,000ᰴ௨ୖ࡛ࠊከᵝ࡞⮫ᗋศ㞳ࡢ೫ᛶ᎘Ẽᛶ⳦ࢆྵࡴࢥࣞࢡࢩࣙࣥ࡜ࡋ࡚ࠊᅜෆ࡟㢮ࢆぢ࡞࠸ࠋࡇࢀࡽࡢ⳦ᰴ࡟ࡘ࠸࡚ࡣᙜ᪋タࡢ㔜せ࡞◊✲ᮦᩱ࡜ࡋ࡚⏝࠸ࡽࢀ࡚࠸ࡿ௚ࠊᅜෆእࡢ኱Ꮫࠊ◊✲ᡤ࡞࡝◊✲ᩍ⫱᪋タࡸࠊ〇⸆࠶ࡿ࠸ࡣヨ⸆ࡢ㛤Ⓨ࡟㛵ಀࡋ࡚࠸ࡿẸ㛫ࡢ◊✲ᡤ࡟ศ୚ࡉࢀ࡚࠾ࡾࠊ◊✲ࠊᩍ⫱ࠊ⏘ᴗ⫱ᡂ࡟᭷ຠ฼⏝ࡉࢀ࡚࠸ࡿࠋ

4㸧་⒪㛵ಀ⪅࡟ᑐࡍࡿ᎘Ẽᛶ⳦࡜᎘Ẽᛶ⳦ឤᰁ⑕࡟㛵ࡍࡿၨⵚάືࢆ✚ᴟⓗ࡟⾜࠺ࠋ௒᪥ࡢ኱Ꮫ་Ꮫ㒊࡟࠾ࡅࡿឤᰁ⑕Ꮫࡢㅮ⩏ࡀෆ⛉Ꮫࡢㅮ⩏඲య࡟༨ࡵࡿ๭ྜࡣࠊᴟࡵ࡚ᑡ࡞࠸ࡇ࡜ࡀࢃ࠿ࡗ࡚࠸ࡿࠋឤᰁ⑕Ꮫࡢ୰࡛ࡶࠊ᎘Ẽᛶ⣽⳦Ꮫ࡟㛵ࡍࡿㅮ⩏ࡢ༨ࡵࡿ๭ྜࡣࡉࡽ࡟ᴟࡵ࡚ᑡ࡞ࡃࠊࡑࡢᩍ⫱ࡢ࡯࡜ࢇ࡝ࡣࠊ༞ᚋᩍ⫱࡟౫Ꮡࡋ࡞ࡅࢀࡤ࡞ࡽ࡞࠸⌧≧࡛࠶ࡿࠋ᎘Ẽᛶ⣽⳦Ꮫ࡜᎘Ẽᛶ⳦ឤᰁ⑕ࡢ⋡ᚋᩍ⫱ࡢࡓࡵࡢࠊᮏ᪋タࡢᙺ๭ࡣࢃࡀᅜ࡟࠾࠸࡚ᴟࡵ࡚኱ࡁࡃࠊᖖ࡟᪂ࡋ࠸᝟ሗࡢ཰㞟࡟ດࡵࠊồࡵ࡟ᛂࡌ࡚ᑐᛂ࡛ࡁࡿែໃࢆᩚ࠼࡚࠸ࡿࠋ

4. ศ㔝᪋タ࡜୺࡞タഛ᎘Ẽᛶ⳦◊✲ศ㔝ࡣࠊ་Ꮫ㒊Ჷ㸵㝵࡟఩⨨ࡍࡿࠋP2 ࣞ࣋ࣝࡢᚤ⏕≀ᐇ㦂ᐊ࡜⣔⤫ಖᏑᐊࢆഛ࠼࡚࠸ࡿࠋ

I. ᎘Ẽᛶࢢ࣮ࣟࣈ࣎ࢵࢡࢫ㸦1 ྎ㸧ࠊ᎘Ẽᛶ࣮࣡ࢡࢫࢸ࣮ࢩࣙࣥ㸦1 ྎ㸧㓟⣲ࢆྵࡲ࡞࠸ΰྜ࢞ࢫ⎔ቃ㸦❅⣲ 82㸣⛬ᗘࠊⅣ㓟࢞ࢫ 8㸣⛬ᗘࠊỈ⣲ 10㸣⛬ᗘ㸧ୗ࡛ࡢసᴗࠊᇵ㣴ࡀྍ⬟ࢸ࣮ࣁ࣮ᘧ࢔ࢼ࢚ࣟ࣎ࢵࢡࢫ ANX-1W ࠙ᖹἑ〇సᡤࠚ௚ 1ྎII. ࢞ࢫ⨨᥮⿦⨨(1 ྎ)㧗ᗘ࡞᎘Ẽ≧ែࢆᚲせ࡜ࡍࡿᇵᆅ➼ࡢㄪᩚ࡟౑⏝ࡍࡿჾᶵ࢞ࢫ⨨᥮⿦⨨(GR-8 ᆺ)࠙୕⤀ᕤᴗࠚ

III. ᚤ⏕≀ྠᐃࢩࢫࢸ࣒(MALDI TOF MS)

MALDI TOF MS ࡟ࡼࡿ⣽⳦࣭┿⳦ࡢྠᐃ⿦⨨ࢹ࣮ࢱ࣮࣋ࢫࡀ࠶ࢀࡤࠊ
㎿㏿࡛㧗⢭ᗘ࡞ྠᐃࡀྍ⬟VITEK MS Plus ࠙ࣅ࣓࣮࢜ࣜࣗࠚ

VI. ⳦ᰴಖᏑ⏝㉸ప ࣇ࣮ࣜࢨ࣮(3 ྎ)5. ᎘Ẽᛶ⳦ಖᏑ⳦ᰴࡢಖ᭷≧ἣa) ᎘Ẽᛶ⳦ࣞࣇ࢓ࣞࣥࢫ⳦ᰴ

⣙290ᰴ

ಖ᭷⳦ᰴᴫせ㸦ᒓ㸧࢔ࢡࢳࣀࣂࢡࢸࣜ࢔㛛I.

Actinobacteria࢔ࢡࢳࣀ࣐࢖ࢭࢫᒓ Actinomycesࣔࣅࣝࣥ࢝ࢫᒓ

Mobiluncus࢟ࣗࢸ࢕ࣂࢡࢸ࣒ࣜ࢘ᒓ Cutibacterium
II. ࣅࣇ࢕ࢻࣂࢡࢸ࣒ࣜ࢘ᒓ Bifidobacterium
III. ࢔ࢺ࣏ࣅ࣒࢘ᒓAtopobiumࢥࣜࣥࢭࣛᒓ Collinsella࢜ࣝࢭࢿࣛᒓ Olsenella
IV. ࢚࣮࢞ࢭࣛᒓ Eggerthellaࢫࣛࢵ࢟࢔ᒓ Slackiaࣇ࢓࣮࣑࣮࢟ࣗࢸࢫ㛛

Firmicutesࢡࣟࢫࢺࣜࢪ࣒࢘ᒓ Clostridium࣌ࣉࢺࢫࢺࣞࣉࢺࢥࢵ࢝ࢫᒓ Peptostreptococcusࣇ࢕ࢿࢦࣝࢹ࢕࢔ᒓ Finegoldiaࣃࣝࣅࣔࢼࢫᒓ Parvimonas࣌ࣉࢺࢽࣇ࢕ࣝࢫᒓ Peptoniphilus࢔ࢿࣟࢥࢵ࢝ࢫᒓ Anaercoccusࣇ࢕ࣜࣇ࢓ࢡࢱ࣮ᒓ Filifactor࣮ࣘࣂࢡࢸ࣒ࣜ࢘ᒓ Eubacteriumࣔࢠࣂࢡࢸ࣒ࣜ࢘ᒓ Mogibacteriumࢩ࣮ࣗࢻ࣑ࣛࢽࣂࢡࢱ࣮ᒓ Pseudoramibacterࢩ࣮ࣗࢻࣇࣛ࣎ࢽࣇࣛࢡࢱᒓ Pseudoflavonifracterࢹ࢕࢔ࣜࢫࢱ࣮ᒓ Dialister࣋࢖ࣚࢿࣛᒓ Veillonellaࣈࣞ࢖ࢹ࢕࢔ᒓ Bulleidiaࢯࣟࣂࢡࢸ࣒ࣜ࢘ᒓ Solobacteriumࢫࢱࣇ࢕ࣟࢥࢵ࢝ࢫᒓ Staphylococcusࢤ࣓ࣛᒓ Gemellaࣛࢡࢺࣂࢳࣛࢫᒓ Lactobacillusࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔㛛Proteobacteriașࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔⥘ Betaproteobacteriaࢧࢵࢸࣞࣛᒓ Sutterellațࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔⥘Deltaproteobacteriaࢹࢫࣝࣇ࢛ࣅࣈࣜ࢜ᒓ Desulfovibrioࣂ࢖࢜ࣇ࢕ࣛᒓ BilophilaȜࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔⥘ Epsilonproteobacteria࢝ࣥࣆࣟࣂࢡࢱ࣮ᒓ Campylobacterࣂࢡࢸࣟ࢖ࢹࢫ㛛

Bacteroidetesࣂࢡࢸࣟ࢖ࢹࢫᒓ Bacteroides࣏ࣝࣇ࢕ࣟࣔࢼࢫᒓ Porphyromonasࣉࣞ࣎ࢵࢸࣛᒓ Prevotella࢝ࣉࣀࢧ࢖ࢺࣇ࢓࣮࢞ᒓ Capnocytophagaࣇࢰࣂࢡࢸࣜ࢔㛛 Fusobacteriaࣇࢰࣂࢡࢸ࣒ࣜ࢘ᒓ Fusobacteriumࣞࣉࢺࢺࣜ࢟࢔ᒓ Leptotrichiab) ྛ✀ឤᰁ⑕ࠊ⑓ែ⏤᮶ࡢ᎘Ẽᛶ⳦⮫ᗋศ㞳ᰴ

⣙9,000ᰴ⳦ᰴ⏤᮶ᴫせ⫹㐨ឤᰁ⑕ ࠊ⭡⭍ෆឤᰁ⑕ࠊ⭠⟶ឤᰁ⑕ࠊ⬻⮋⒆ࠊ⳦⾑⑕ࠊ⓶⭵㌾㒊⤌⧊ឤᰁ⑕ࠊࡑࡢ௚ࡢ
⏘፬ே⛉ឤᰁ⑕ࠊ⪥㰯ဗႃ⛉ឤᰁ⑕ࠊཱྀ⭍እ⛉㡿ᇦឤᰁ⑕ࠊ࿧྾ჾ⛉㡿ᇦឤᰁ⑕ࠊᩚᙧእ⛉ឤᰁ⑕ࠊ౽ࠊ㰯⭍ࢧࣥࣉࣝࠊ㝔ෆ⎔ቃಖ᭷⳦ᰴᴫせ㸦ᒓ㸧࢔ࢡࢳࣀࣂࢡࢸࣜ࢔㛛I.

Actinobacteria࢔ࢡࢳࣀ࣐࢖ࢭࢫᒓ

Actinomycesࣔࣅࣝࣥ࢝ࢫᒓ Mobiluncus࢟ࣗࢸ࢕ࣂࢡࢸ࣒ࣜ࢘ᒓ CutibacteriumII.

ࣅࣇ࢕ࢻࣂࢡࢸ࣒ࣜ࢘ᒓ Bifidobacterium࣮࢞ࢻࢿࣞࣛᒓ Gardnerellaࣃࣛࢫ࢝ࣝࢻࣅ࢔ᒓ

Parascardoviaࢫ࢝ࣝࢻࣅ࢔ᒓ Scardovia



III.

IV.

࢔ࢺ࣏ࣅ࣒࢘ᒓ AtopobiumࢥࣜࣥࢭࣛᒓCollinsella࢜ࣝࢭࢿࣛᒓ

Olsenella࢚࣮࢞ࢭࣛᒓ

Eggerthellaࢫࣛࢵ࢟࢔ᒓ

Slackiaࣇ࢓࣮࣑࣮࢟ࣗࢸࢫ㛛

Firmicutesࢡࣟࢫࢺࣜࢪ࣒࢘ᒓ Clostridium࣌ࣉࢺࢫࢺࣞࣉࢺࢥࢵ࢝ࢫᒓ Peptostreptococcusࣇ࢕ࢿࢦࣝࢹ࢕࢔ᒓ Finegoldiaࣃࣝࣅࣔࢼࢫᒓ Parvimonas࣌ࣉࢺࢽࣇ࢕ࣝࢫᒓ Peptoniphilus࢔ࢿࣟࢥࢵ࢝ࢫᒓ Anaercoccusࣇ࢕ࣜࣇ࢓ࢡࢱ࣮ᒓ Filifactor࣮ࣘࣂࢡࢸ࣒ࣜ࢘ᒓ Eubacteriumࣔࢠࣂࢡࢸ࣒ࣜ࢘ᒓ Mogibacteriumࢩ࣮ࣗࢻ࣑ࣛࢽࣂࢡࢱ࣮ᒓ

Pseudoramibacterࢩ࣮ࣗࢻࣇࣛ࣎ࢽࣇࣛࢡࢱᒓ Pseudoflavonifracterࢹ࢕࢔ࣜࢫࢱ࣮ᒓ Dialister࣋࢖ࣚࢿࣛᒓ Veillonellaࣈࣞ࢖ࢹ࢕࢔ᒓ Bulleidiaࣈࣛ࢘ࢸ࢕࢔ᒓ Blautiaࢯࣟࣂࢡࢸ࣒ࣜ࢘ᒓ Solobacteriumࢫࢱࣇ࢕ࣟࢥࢵ࢝ࢫᒓ Staphylococcusࢤ࣓ࣛᒓ Gemellaࣛࢡࢺࣂࢳࣛࢫᒓ Lactobacillusࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔㛛Proteobacteriașࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔⥘Betaproteobacteriaࢧࢵࢸࣞࣛᒓ Sutterella࢚࢖ࢣࢿࣛᒓ Eikenellațࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔⥘Deltaproteobacteriaࢹࢫࣝࣇ࢛ࣅࣈࣜ࢜ᒓ Desulfovibrioࣂ࢖ࣟࣇ࢕ࣛᒓ BilophilaȜࣉࣟࢸ࢜ࣂࢡࢸࣜ࢔⥘Epsilonproteobacteria࢝ࣥࣆࣟࣂࢡࢱ࣮ᒓ Campylobacterࣂࢡࢸࣟ࢖ࢹࢫ㛛

Bacteroidetesࣂࢡࢸࣟ࢖ࢹࢫᒓ Bacteroides࣏ࣝࣇ࢕ࣟࣔࢼࢫᒓ Porphyromonasࣉࣞ࣎ࢵࢸࣛᒓ Prevotella࢝ࣉࣀࢧ࢖ࢺࣇ࢓࣮࢞ᒓ Capnocytophagaࣇࢰࣂࢡࢸࣜ࢔㛛 Fusobacteriaࣇࢰࣂࢡࢸ࣒ࣜ࢘ᒓ Fusobacteriumࣞࣉࢺࢺࣜ࢟࢔ᒓ Leptotrichiaࢫࣆࣟࢣ࣮ࢱ㛛 Spirochaetesࣈࣛ࢟ࢫࣆࣛᒓ Brachyspiraࢩࢿࣝࢠࢫࢸࢫ㛛 Synergistetesࢩࢿࣝࢠࢫࢸࢫᒓ Synergistes

ᖺᗘูෆヂ
ᖺ

GAI No.

཰㞟ᰴᩘ1991ࠥ2008(# 91000㹼08503)

7119

2009

(# 09001㹼09209)

209

2010

(# 10001㹼10202)

202

2011

(# 11001㹼11207)

207

2012

(# 12001㹼12149)

149

2013

(# 13001㹼13107)

107

2014

(# 14001㹼14227)

227

2015

(# 15001㹼15240)

240

2016

(# 16001㹼16135)

135

2017

(# 17001㹼17313)

313

2018

(# 18001㹼18160)

160

2019

(# 19001㹼19029)

29

2020

(# 20001㹼20216)

216

2021

(# 21001㹼21144)

144

6. ศ㔝ࡢᴗົ࡜ᨭ᥼1) ⳦ᰴ⥔ᣢ࣭⣔⤫ಖᏑ2) ᎘Ẽᛶ⳦ࡢ⏕ែ࡞࡝࡟㛵ࡍࡿၥ࠸ྜࢃࡏ࡬ࡢᑐᛂ3) ᇵ㣴ἲ࡞࡝◊✲ୖࡢᢏ⾡ⓗ࡞┦ㄯ࡬ࡢᑐᛂ4) ᎘Ẽ⎔ቃࢆᚲせ࡜ࡍࡿ◊✲ࡢᨭ᥼5) ᎘Ẽᛶ⳦ࢆ୰ᚰ࡜ࡋࡓ⣽⳦ྠᐃࠊឤཷᛶ ᐃ6) ᎘Ẽᛶ⳦ࡢᅜෆእࡢ◊✲⪅࡟ᑐࡍࡿศㆡ㸦⟶⌮యไ࣭タഛࡀᩚࡗ࡚࠸ࡿ◊✲ᐊᑐ㇟㸧᪥ᮏ⣽⳦Ꮫ఍ᩍ⫱⏝⳦ᰴࡢศ୚
᪥ᮏ໬Ꮫ⒪ἲᏛ఍MIC ᐃጤဨ఍ᣦᐃࢥࣥࢺ࣮ࣟࣝ⳦ᰴࡢศ୚ྛ✀ྠᐃ⏝࢟ࢵࢺࡢ⢭ᗘ⟶⌮⏝⳦ᰴࡢศ୚
ᢠ⳦⸆ࠊヨ⸆㛤Ⓨࡢࡓࡵࡢ⳦ᰴࡢศ୚7) ⮫ᗋ᎘Ẽᛶ⳦࡟ࡘ࠸࡚ࡢㅮ⩦఍㛤ദ᎘Ẽᛶ⳦᳨ᰝᢏ⾡ࢭ࣑ࢼ࣮

7. ᨭ᥼ࡢᐇ⦼

㸦1㸧┦ㄯ࣭ၥྜࡏ᳨࣭ᰝ౫㢗௧࿴ 3 ᖺᗘ࣭⮫ᗋᮦᩱ࠿ࡽศ㞳ࡉࢀࡓ᎘Ẽᛶ⳦ࡢヲ⣽ྠᐃ࡜⸆๣ឤཷᛶヨ㦂㸸 1௳2ᰴ(┴እ་⒪ᶵ㛵)࣭⮫ᗋᮦᩱ࠿ࡽศ㞳ࡉࢀࡓ᎘Ẽᛶ⳦ࡢヲ⣽ྠᐃ㸸⾑ᾮᇵ㣴ศ㞳⳦ 1௳㸦┴ෆ་⒪ᶵ㛵㸧࣭┦ㄯ࠙◊✲ᡭἲࠊᇵ㣴ࠊྠᐃࠊឤཷᛶ ᐃἲ࡞࡝ᐇ㦂ୖࡢᢏ⾡ⓗ࡞┦ㄯ࡬ࡢᑐᛂࠚ㸸Ꮫෆ 1 ௳ࠊ┴እ኱Ꮫ㸦་⒪⣔㸧1 ௳ࠊ┴እ኱Ꮫ 1 ௳࣭ၥ࠸ྜࢃࡏ ࠙⑓ཎ⳦ࡢ⏕ែ࡟ࡘ࠸࡚ࠚ┴እ኱Ꮫ1 ௳ࠊ┴እ⮬἞య 2 ௳࠙᎘Ẽᛶ⳦ࡢྠᐃ࡟ࡘ࠸࡚ࠚ┴እ኱Ꮫ 1 ௳࠙⳦✀ྡ࡟ࡘ࠸࡚ࠚ┴እ௻ᴗ 1 ௳࣭⳦ᰴࡢ⥔ᣢ Ꮫෆ 1 ௳࣭ᢏ⾡ᥦ౪ 2 ௳ ┴ෆ௻ᴗ

㸦2㸧᪂つಖᏑᩘ 114 ᰴ
㸦3㸧⳦ᰴศㆡ ྛ✀᎘Ẽᛶ⳦ 6 ᰴ
㸦4㸧⮫ᗋ᎘Ẽᛶ⳦࡟ࡘ࠸࡚ࡢㅮ⩦఍㛤ദ௒᪥ࡢ኱Ꮫ་Ꮫ㒊࡟࠾ࡅࡿឤᰁ⑕Ꮫࡢㅮ⩏ࡀෆ⛉Ꮫࡢㅮ⩏඲య࡟༨ࡵࡿ๭ྜࡣࠊᴟࡵ࡚ᑡ࡞࠸ࡇ࡜ࡀࢃ࠿ࡗ࡚࠸ࡿࠋࡲࡓࠊឤᰁ⑕Ꮫࡢ୰࡛ࡶࠊ᎘Ẽᛶ⣽⳦Ꮫ࡟㛵ࡍࡿㅮ⩏ࡢ༨ࡵࡿ๭ྜࡣࡉࡽ࡟ᴟࡵ࡚ᑡ࡞ࡃࠊࡑࡢᩍ⫱ࡢ࡯࡜ࢇ࡝ࡣࠊ༞ᚋᩍ⫱࡟౫Ꮡࡋ࡞ࡅࢀࡤ࡞ࡽ࡞࠸⌧≧࡛࠶ࡿࠋ᳨ᰝᢏᖌࡢᩍ⫱࡟㛵ࡋ࡚ࡶྠᵝࡢࡇ࡜ࡀゝ࠼ࡿࠋ᎘Ẽᛶ⣽⳦Ꮫ࡜᎘Ẽᛶ⳦ឤᰁ⑕ࡢ༞ᚋᩍ⫱࡟࠾ࡅࡿᮏ᪋タࡢᙺ๭ࡣࠊࢃࡀᅜ࡟
࠾࠸࡚ᴟࡵ࡚኱ࡁ࠸࡜⪃࠼ࡽࢀࡿࠋ᎘Ẽᛶ⳦ឤᰁ⑕ࡣࠊෆ⛉ࠊእ⛉ࠊᩚᙧእ⛉ࠊ⏘፬ே⛉ࠊ⪥㰯ဗႃ⛉ࠊṑ⛉ཱྀ⭍እ⛉࡞࡝ᖜᗈ࠸㡿ᇦ࡛ぢࡽࢀࠊ᎘Ẽᛶ⳦ࡢ⮫ᗋ᳨ᰝࡣឤᰁ⑕ࡢデ᩿࣭἞⒪࡟㔜せ࡛࠶ࡿࠋᮏࢭ࣑ࢼ࣮ࡣࠊ᎘Ẽᛶ⳦࠾ࡼࡧ᎘Ẽᛶ⳦ឤᰁ⑕࡟⯆࿡ࡢ࠶ࡿ᪉ࠎ࡟ࠊ᭱᪂ࡢ᝟ሗࢆ஺࠼ࡓ඲⯡ⓗ࡞▱㆑࡜᳨ᰝ࡟㛵ࢃࡿᢏ⾡ࢆ⩦ᚓࡋ࡚㡬ࡃࡇ࡜ࢆ┠ⓗ࡜ࡋ࡚ㅮ⩏࡜ᐇ⩦ࢆᐇ᪋ࡋ࡚࠸ࡿࠋࠓ➨ 49 ᅇ᎘Ẽᛶ⳦᳨ᰝࢭ࣑ࢼ࣮ࠔࡲ࡛ࠊ⥲ཧຍேᩘ㸸⣙ 1747 ྡࠓ➨ 50 ᅇ᎘Ẽᛶ⳦᳨ᰝࢭ࣑ࢼ࣮ࠔ
㛤ദᮇ㛫㸸௧࿴ 3 ᖺ 7 ᭶ 30 ᪥㸦㔠㸧㹼8 ᭶ 1 ᪥㸦᪥㸧 㸦ᐃဨ 20 ྡ㸧ཧຍ⪅㸸22 ྡ㸦་⒪ᶵ㛵 19ࠊ᳨ᰝࢭࣥࢱ࣮2ࠊ௻ᴗ 1㸹⮫ᗋ᳨ᰝ㛵ಀ 20 ྡࠊ⸆๣ᖌ 1 ྡࠊ◊✲⪅ 1㸹9 㒔㐨ᗓ┴㸧

8. ᩍဨࡢ◊✲࣭ᩍ⫱࣭♫఍άື࠙ㄽᩥ➼ࠚཎⴭ㸦Ḣᩥ㸧
1.

Vu H, Hayashi H, Nguyenn TN, Kong DT, Tran HT, Yamamoto Y, Tanaka K. Comparison ofphenotypic and genotypic patterns of antimicrobial-resistant Bacteroides fragilis group isolatedfrom healthy individual in Vietnam and Japan. Infection and Drug Resistance, 14:5313-5323,(2021)
2.

KimuraI,VuH,

TanakaK.

Multiplicityof

antimicrobial anti-inflammatory andanti-proliferative effects with a combined formulation of extracts of Bamboo Leaf, Red Pine
Needle Leaf and Ginseng Root. Pharmacometrics,101(3/4);37-44,(2021)
3.

Sano H, Waku A, Hirabuki Y, Kawachi M, Washino J, Abiko Y, Mayanagi G, Yamaki K, TanakaK, Takahashi N, Sato T. Profiling system of oral microbiota utilizing polymerase chain
reaction-restriction fragment length polymorphism analysis. Journal of Oral Biosciences.
63(3):292-297,(2021)

4.

Wakui A, Sano H, Kawachi M, Aida A, Takenaka Y, Yonezawa A, Nakahata N, Moriyama S,
Nishikatab M, Washio J, Abiko Y, Mayanagi G, Yamaki K, Sakashita R, Tanaka K, Takahashi
N, Sato T. Bacterial concentration and composition in liquid baby formula and a baby drink
consumed with an artificial nipple. Journal of Oral Biosciences. 63:161-168,(2021)

5.

Wakui A, Sano H, Hirabuki , Kawachi M, Aida A, Washio J, Abiko Y, Mayanagi G, Yamaki K,
Tanaka K, Takahashi and Sato T. Profiling of Microbiota at the Mouth of Bottles and inRemaining Tea after Drinking Directly from Plastic Bottles of Tea. Dentistry journal. 9(6),58,(2021)

6.

Tanaka K, Vu H, Hayashi M. In vitro activities and spectrum of lascufloxacin㸦KRP-AM1977㸧against anaerobes. Journal of Infection and Chemotherapy. 27:1265-1269,(2021)࠙Ꮫ఍ࠚᅜ㝿Ꮫ఍
1. World Microbe Forum 2021㸦௧࿴ 3 ᖺ 6 ᭶ 20㹼24 ᪥, WEB, ࣏ࢫࢱ࣮ࢭࢵࢩࣙࣥࠕCharacterizationof cODVV'ǃ-lactamase from Bacteroides fragilis GAI92214, a strain intermediately resistant tocarbapenemsࠖ₇⪅㸧Goto T, Hayashi M, Morita Y, Tanaka K.

ᅜෆᏛ఍
1. ➨ 50 ᅇ᪥ᮏ᎘Ẽᛶ⳦ឤᰁ⑕Ꮫ఍⥲఍㸦௧࿴ 3 ᖺ 5 ᭶ 29 ᪥㸪WEB㸪ࢩ࣏ࣥࢪ࣒࢘ ࠕ᎘Ẽᛶ⳦◊✲ࡢ᭱๓⥺ࠖྖ఍㸧⏣୰㤶࠾㔛
2. ➨ 50 ᅇ᪥ᮏ᎘Ẽᛶ⳦ឤᰁ⑕Ꮫ఍⥲఍㸦௧࿴ 3 ᖺ 5 ᭶ 29 ᪥㸪WEB㸪ࢩ࣏ࣥࢪ࣒࢘ ࠕ᎘Ẽᛶ⳦◊✲ࡢ᭱๓⥺ࠖ-ࠕMALDI-TOF MS ࢆ⏝࠸ࡓ Fusobacterium ᒓࡢள✀ྠᐃ࡟㛵ࡍࡿ᳨ウࠖ₇⪅㸧ᯘᑗ኱

3. ➨ 69 ᅇ᪥ᮏ໬Ꮫ⒪ἲᏛ఍す᪥ᮏᨭ㒊⥲఍࣭➨ 64 ᅇ᪥ᮏឤᰁ⑕Ꮫ఍୰᪥ᮏᆅ᪉఍Ꮫ⾡㞟఍࣭➨ 91ᅇ᪥ᮏឤᰁ⑕Ꮫ఍す᪥ᮏᆅ᪉఍Ꮫ⾡㞟఍㸦௧࿴ 3 ᖺ 11 ᭶㸪ᒱ㜧ᕷ㸪ᩍ⫱ㅮ₇ 11ࠕᚤ⏕≀ศ㔝࡟࠾ࡅࡿḟୡ௦ࢩ࣮ࢡ࢚ࣥࢫࡢά⏝ࠖྖ఍ࠊࢫ࢖࣮ࢶࢭ࣑ࢼ࣮3ࠕ࿧྾ჾឤᰁ⑕ࠊ⪥㰯ဗႃ⛉ឤᰁ⑕᳨࡛ฟࡉࢀࡿ᎘Ẽᛶ⳦ࠖ ₇⪅㸧⏣୰㤶࠾㔛
4. ➨ 33 ᅇ᪥ᮏ⮫ᗋᚤ⏕≀Ꮫ఍⥲఍(௧࿴ 4 ᖺ 1 ᭶ 29 ᪥ࠥ3 ᭶ 31 ᪥, ௝ྎᕷ, ᩍ⫱ㅮ₇ 11ࠕ࡝ࡇࡀ࣏࢖ࣥࢺ㸽 ᎘Ẽᛶ⳦ 㸫ຠ⋡ࡼࡃ⮫ᗋ࡟ά࠿ࡍࡓࡵ࡟㸫ࠖ₇⪅ࠊࢩ࣏ࣥࢪ࣒࢘ 7ࠕ᎘Ẽᛶ⳦᳨ᰝࡢὀពⅬ࡜ឤᰁ⑕ࠖྖ఍) ⏣୰㤶࠾㔛
5. ➨ 33 ᅇ᪥ᮏ⮫ᗋᚤ⏕≀Ꮫ఍⥲఍(௧࿴ 4 ᖺ 1 ᭶ 29 ᪥ࠥ3 ᭶ 31 ᪥, ௝ྎᕷ,ࢩ࣏ࣥࢪ࣒࢘ 7ࠕ᎘Ẽᛶ⳦᳨ᰝࡢὀពⅬ࡜ឤᰁ⑕ࠖ₇⪅) ᯘᑗ኱ཷ㈹᪥ᮏ᎘Ẽᛶ⳦ឤᰁ⑕Ꮫ఍ ᴟᮾ〇⸆㈹ࠕMALDI-TOF MS ࢆ⏝࠸ࡓ Fusobacterium ᒓࡢள✀ྠᐃ࡟㛵ࡍࡿ᳨ウࠖ ᯘᑗ኱࠙ඹྠ◊✲ࠚ1 ௳ ᎘Ẽᛶ⳦ಖᏑ㍺㏦ᐜჾࡢホ౯ヨ㦂 㸦┴እ௻ᴗ㸧௧࿴ 3 ᖺ 12 ᭶㹼௧࿴ 4 ᖺ 5 ᭶࠙ᩍ⫱ศᢸࠚ⏣୰㤶࠾㔛㸸㐃ྜ๰⸆་⒪᝟ሗ◊✲⛉㸦ే௵㸧་Ꮫ㒊་Ꮫ⛉ ࢸ࣮ࣗࢺࣜ࢔ࣝ ࢸ࣮ࣗࢱ࣮་Ꮫ㒊་Ꮫ⛉ 㑅ᢥࢸ࣮ࣗࢺࣜ࢔ࣝ
඲Ꮫඹ㏻ᩍ⫱ㅮ⩏་Ꮫ㒊┳ㆤᏛ⛉ ㅮ⩏ᚋ⸨ 㝯ḟ㸸་Ꮫ㒊་Ꮫ⛉ࢸ࣮ࣗࢺࣜ࢔ࣝ ࢸ࣮ࣗࢱ࣮་Ꮫ㒊་Ꮫ⛉ 㑅ᢥࢸ࣮ࣗࢺࣜ࢔ࣝᯘ ᑗ኱㸸་Ꮫ㒊་Ꮫ⛉ࢸ࣮ࣗࢺࣜ࢔ࣝ ࢸ࣮ࣗࢱ࣮་Ꮫ㒊་Ꮫ⛉ 㑅ᢥࢸ࣮ࣗࢺࣜ࢔ࣝ