LAS X
for Confocal Systems

Release Notes LAS X 3.5.7

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Release documentation for LAS X 3.5.7
This document describes the 3.5.7 release of the Leica Application Suite X imaging
and analysis software for advanced live cell research. You should read this document
before installing a copy of this software.
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Date: October 16th, 2020 applying to build number 23225

LAS X 3.5.7 Release Notes for Confocal Systems

2Contents
1. Compatible Microscopes and Technical Requirements ............................................................................ 4
1.1 Compatible Microscopes and Hardware Requirements .................................................................. 4
1.2 Operating System Requirements ........................................................................................................ 4
2. Reason for this Release – LAS X 3.5.7 ........................................................................................................ 4
3. Recommendations .......................................................................................................................................... 4
4. Open Issues / Restrictions ............................................................................................................................. 5
5. Solved Issues / Restrictions ........................................................................................................................... 6

LAS X 3.5.7 Release Notes for Confocal Systems3

1. Compatible Microscopes and Technical Requirements
1.1 Compatible Microscopes and Hardware Requirements
The LAS X 3.5.7 control software is a release for legacy confocal systems running on
LAS X, including the SP8 confocal platform, the SPE and the LSI. Accordingly, it is notcompatible with the new STELLARIS confocal platform and legacy confocal systems
not capable of running LAS X (i.e., systems running LAS-AF such as the SP5). All
Leica-supplied workstations for the respective confocal platform meet the minimum
PC requirements of the LAS X 3.5.7 control software.

1.2 Operating System Requirements
LAS X 3.5.7 is a genuine 64-bit program and runs on Windows 7 64bit and on Windows
10 64bit.

2. Reason for this Release – LAS X 3.5.7
LAS X 3.5.7 is a release to address vulnerabilities in a 3rd party application (WIBU
CodeMeter) shipped with LAS X. Importantly, LAS X 3.5.7 also entails several major
bugfixes addressing the usability of LAS X 3.5.6, namely for the Navigator, DyeAssistant and FALCON.

3. Recommendations
For an optimal performance and the safety of the product, we strongly recommend theinstallation of/upgrade to LAS X 3.5.7 for SP8, SPE and LSI systems!

LAS X 3.5.7 Release Notes for Confocal Systems

44. Open Issues / Restrictions
The following list enumerates the known product limitations of LAS X 3.5.7:

Navigator: Limited capabilities for water dispenser

Workaround: In the Navigator, the water dispenser cannot pump water during
TileScan- and xyt-scans. As workaround, the optional HCS-A package can be used
for controlling the water dispenser in stage application experiments.

Z-stacks can be slower with z-Flow (GalvoFlow) enabled

Workaround: The z-Flow enables maximum acquisition speed in experiments
entailing the z-dimension. However, for certain experimental settings (xyzt-scanmode with few z-planes) it is possible that the acquisition is faster with disabled zFlow. The experimenter can guarantee to use the highest acquisition speed bycomparing the total experimental time with and without z-Flow.

TauSTED: Decay curve occasionally missing

Workaround: Occasionally, the decay curve for TauSTED experiments is notshown. In this case, the user can click on “FLIM” to show the decay curve.

Error during export of multiple LIF files in parallel

Workaround: Exporting multiple LIF files is not supported. Thus, users must export
LIF files sequentially. Furthermore, the LIF format is widely supported (including
3rd party and open source software such as ImageJ, MATLAB or Python).

LDM does not apply z-compensation for laser correctly in multicolor loops

Workaround: If LDM is set up with two colors, the z-compensation value from thelast image will be applied from the second loop on, i.e. also in the first channel this
fixed value of laser power would be wrongly applied. For most applications, there

LAS X 3.5.7 Release Notes for Confocal Systems

5are two workarounds available: (i) Split the multicolor experiment into distinct LDM
jobs (then the z-compensation will be applied correctly in loops). (ii) Alternatively,
use the Navigator and its z-compensation.

FCS: Bleach point defined in xz-scan not used for data acquisition

Workaround: For most applications, it is acceptable to set up different FCS
measurements at different z-positions.

5. Solved Issues / Restrictions
Amongst other bugfixes, we have resolved the following issues/restrictions from theLAS X 3.5.6 release:Dye Assistant: Cannot choose detector type

Navigator: Save overview (preview) not working

Navigator: Frame Averaging / Frame Accumulation not available



Navigator: Toggling between main wizard and Navigator (leads to blackscreen)

Navigator: Merging leads to intensity changes (at tile borders)Navigator: Lost z-compensation window when closing Navigator with open zcompensation dialogue (until LAS X restart)

FALCON: xt-scans not available



FALCON: FLIM analysis leads to blank data when ROI applied to 2nd or 3rd
channel of a line-by-line sequential scan

FALCON: No photons after drawing ROI on frame sequential experiment(“Attempted to divide by zero” error)

LAS X 3.5.7 Release Notes for Confocal Systems6