ONCOLOGYLung Cancer
Mutation
Profiling

For Research Use Only.
Not for use in diagnostic procedures.

Minimize Sample Rejection in Lung Cancer Testing
Mutation profiling of non-small cell lung cancer samples can be challenging due to limited quantity & poor
quality of the specimens. Several technologies, including next generation sequencing, require specimens
with high tumor content and generally reject as many as 20% of the samples due to low tumor fraction
and DNA yield.1, 2

AGENA'S SOLUTION
UltraSEEK™ Lung Panel
Enables study of disease progression and resistance from CTCs and ctDNA, detecting 74 variantsfrom a single blood draw at as low as 0.1% minor allele frequency (MAF). Optimized for low and poorquality DNA, reducing sample rejection and workflow failure rate.
iPLEX® HS Lung Panel
Facilitates variant detection as low as 1% MAF from poor quality and degraded samples such as
FFPE tissue, fine needle aspirates, core needle biopsies, smears, pleural fluid and cytology blocks.
UltraSEEK EGFR Panel
Conduct a focused study across clinically relevant variants in theEGFR gene including p.T790M, p.C797S , p.E746_A750del and p.L858R.

For Research Use Only. Not for use in diagnostic procedures.

ONCOLOGY

Genes & Mutations for Lung Panels
Pre-designed panels with content compliant with the NCCN guidelines across 5 genes forcomprehensive profiling of lung cancer.
UltraSEEK Lung Panel
GeneCoverage*

BRAF

Codons 469 (exon 11), 594, 600 (exon 15)

4

EGFR

Exon 19 indels, exon 20 insertions and substitutions across exons 18, 19, 20 and 21

46ERBB2

Exon 20 insertions

4KRAS

Codons 12, 13 (exon 2) and 61 (exon 3)

16PIK3CA

Codons 542, 545 (exon 9) and 1047 (exon 20)# of Variants*

4

Total Variants

74

iPLEX HS Lung Panel
GeneCoverage*

BRAF

Codons 469 (exon 11), 594, 600 (exon 15)

4EGFR

Exon 19 indels, exon 20 insertions and substitutions across exons 18, 19, 20 and 21

46ERBB2

Exon 20 insertions

2

KRAS

Codons 12, 13 (exon 2) and 61 (exon 3)

14

PIK3CA

Codons 542, 545 (exon 9) and 1047 (exon 20)

Total Variants# of Variants*

470

UltraSEEK EGFR Panel
GeneCoverage*

EGFR

Exon 19 deletions and substitutions across Exon 20 & 21

Total Variants# of Variants*66* Complete variant list available upon request

ASSAY WORKFLOW
DNA to data in as little as 8 hours, with minimal manual processing time enables greater lab efficiency.
Simplified reporting with automated software generates clear results.

ORDERING INFORMATION
Catalog No.

Item

Sample Type13136F

Complete UltraSEEK Lung Panel Set CPM

13138F

Complete UltraSEEK Lung Panel Set CPM# Samples

Chip Format

Plasma32

96

Plasma80

9613124F

Complete iPLEX HS Lung Panel Set CPM

Tissue489613126F

Complete iPLEX HS Lung Panel Set CPMTissue

120

96

Tissue

48

384

13128

Complete iPLEX HS Lung Panel Set

13124

Complete iPLEX HS Lung Panel Set

Tissue

4896

13126

Complete iPLEX HS Lung Panel Set

Tissue1209613129

Complete iPLEX HS Lung Panel Set

Tissue14438413236F

Complete UltraSEEK EGFR Panel Set CPM

Plasma192

96

The panel sets contain assay specific primers and all the required reagents to process DNA samples on the MassARRAY® system.

References
1. R. Avula et al. Assessment of UltraSEEK Colon Cancer Panel for Detection of Low Frequency Somatic Mutations in Blood. Poster session presented at:
Association of Molecular Pathology Annual Meeting; 2017 Nov 16-18; Salt Lake City, UT.
2. R.T. Birse, D. Irwin. Reliable Detection of Low Abundance Somatic Mutations of EGFR, KRAS, BRAF, NRAS and PIK3CA in Metastatic Colorectal
Adenocarcinomas Using iPLEX HS, a New Highly Sensitive Assay for MassARRAY. Poster session presented at Association of Molecular Pathology AnnualMeeting; 2016 Nov 10-12; Charlotte, NC.

For Research Use Only. Not for use in diagnostic procedures.

Agena Bioscience, Inc.
4755 Eastgate Mall
San Diego, CA 92121
Phone: +1.858.882.2800

ONC0005 03Order Desk: +1.858.202.9301
Order Desk Fax: +1.858.202.9220
orderdesk@agenabio.com
Web: agenabio.com

USEUAP
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MassARRAY, iPLEX and Agena Bioscience are registered trademarks of Agena Bioscience, Inc.
UltraSEEK is a trademark of Agena Bioscience, Inc. ©2019 Agena Bioscience, Inc. All rights reserved.