From Eye to Insight

LAS X
for Widefield

Release Notes LAS X 3.6.1

LAS X 3.6.1 Release Notes for Widefield Systems

1Release documentation for LAS X 3.6.1
This document describes the 3.6.1 release of the Leica Application Suite X imaging andanalysis software for life science research and applied microscopy. You should read thisdocument before installing your copy of this software.
All reasonable steps have been taken to ensure that this publication is correct and complete,
but should any user be in doubt about any detail, clarification may be sought from Leica
Microsystems CMS GmbH, or their accredited representative. The information in thisdocument is subject to change without notice and should not be construed, as a commitment
by Leica Microsystems CMS GmbH. Leica Microsystems CMS GmbH accepts no
responsibility for any errors that may appear in this document.

Copyright © 2018 Leica Microsystems CMS GmbHAll rights reserved. The contents of this publication may not be reproduced in any form, orcommunicated to a third party without prior written permission of Leica Microsystems CMS
GmbH. Due to a policy of continuous development, we reserve the right to change
specifications without notice. Microsoft and MS-DOS are registered trademarks and Windows,
the Windows logo, the Windows 2000 logo, the Windows XP logo, the Windows Vista logo,
the Windows 7 logo and the Windows 10 logo are trademarks of Microsoft Corporation.

Date: October 16, 2020, applying to build number 23246

LAS X 3.6.1 Release Notes for Widefield Systems

2

Contents
1.

2.

Compatible Microscopes and Cameras ...................................................................... 4
1.1

Compatible Microscopes ..................................................................................... 4

1.2

Compatible Cameras ........................................................................................... 6

Technical Requirements and Installation .................................................................... 8
2.1

Compatibility to LAS, LAS AF, former LAS X versions and LMD ......................... 8

2.2

Installing the Correct Firmware Versions ........................................................... 10

2.3

PC Requirements .............................................................................................. 12

3.

Product Security Fix ................................................................................................. 13

4.

Important Information ............................................................................................... 13

LAS X 3.6.1 Release Notes for Widefield Systems

31. Compatible Microscopes and Cameras
1.1

Compatible Microscopes

Microscope/ System

CompaComments
tibility

Inverted Microscopes
Leica DMi8
Leica DMi8 C/ A
Leica DMI6000 B/ with AFC
Leica DMI4000 B
Leica DMI3000 B
Leica DM IL

YES
YES
YES
YES
YES
YES

Upright Research Microscopes
Leica DM4 B
Leica DM6 B
Leica DM6000 B
Leica DM5500 B
Leica DM5000 B

YES
YES
YES
YES
YES

Leica DM4500 B

YES

Leica DM4000 B

YES

stands with BZ >= 03 only
stands with production date >=2008
onlystands with production date >=2008
onlyFixed Stage Microscopes
Leica DM6 FS
Leica DM6000 FS

YES
YES

Stereos and Macroscopes
Leica M205 C/ A/ FA/ FCA
Leica M165 C/ FC

YES
YES

Upright Routine Microscopes
Leica DM3000 / LED
Leica DM2000 / LED
Leica DM1750
Leica DM1000 / LED

YES
YES
YES
YES

Educational Microscopes
Leica DM750
Leica DM500

YES
YES

LAS X 3.6.1 Release Notes for Widefield Systems

4

Leica EZ4E

YES

only USB connection, Ethernet notsupported

Leica EZ4W

YES

only USB connection, Ethernet and Wifi
not supported

Infinity Port Modules
Infinity TIRF
Infinity TIRF HP
Infinity Scanner
Infinity Scanner FS
WF FRAP
Combination PLU and WSU

YES
YES
YES
YES
YES
YES

TIRF and GSD Systems
Leica AM TIRF MC
Leica AM TIRF MC with AFC
Leica SR GSD

YES
YES
NO

THUNDER Imagers
THUNDER Imager | Tissue
THUNDER Imager | 3D Tissue
THUNDER Imager | Cell Culture
THUNDER Imager | Live Cell
THUNDER Imager | Model Organism

YES
YES
YES
YES
YES

CLEM

YES

LAS X 3.6.1 Release Notes for Widefield Systems

5

1.2

Compatible Cameras

LAS X 3.6.1 Release Notes for Widefield Systems

6

USB3 cameras:
Leica USB3 cameras are tested with USB3 PCIe cards from DeLock. These cards have a
Renesas PD720202 chipset. Leica strongly recommends to use these cards. The majority of
the Leica USB3 cameras are delivered with this USB3 PCIe card.
If you plan to use a different card or an onboard-USB port with a different chipset, please makesure you have the latest drivers from the card manufacturer and the latest Windows updates
installed. Leica does not assume any liability when not using the recommended USB3 PCIe
card.
Please also branch the internal power supply when using a card.
Due to the high power demands of some cameras, and the large amounts of data they deliver,
Leica recommends to use each camera on a separate card. Leica recommends not to use
other instruments with high power requirements, such as external harddrives, on the same
USB socket.
Some computers have a USB3 charging port (USB3 with a flash sign). We do not recommend
to use this port, since the camera will never power off and can prevent the computer fromrebooting.

LAS X 3.6.1 Release Notes for Widefield Systems

7

2. Technical Requirements and Installation
Warnings
Warning!! LAS X 3.6.1 is a Widefield Research release only. Do notinstall on Confocal systems nor Applied Microscopy systems.

Operating System
LAS X 3.6.1 is a genuine 64 bit program and runs on Windows 10 and Windows 7 64-bit
only.

2.1
LMD

Compatibility to LAS, LAS AF, former LAS X versions and2.1.1 Compatibility to former LAS X versions
Former LAS X versions on widefield research systems can be upgraded with LAS X
3.6.1.
Former LAS X versions on EM Cryo CLEM systems can be upgraded with LAS X 3.6.1,
but the performance of the PC might be not good enough to have a smooth software
process.

2.1.2 Compatibility to LAS
LAS X 3.6.1 can read and process single images generated with LAS versions.
However, image series generated with LAS need to be imported with the LAS X fileimporter by entering the metadata manually.
LAS versions cannot read data acquired with LAS AF or LAS X software versions.

2.1.3 Compatibility to LAS AF versions
All LAS AF 4 versions can be upgraded with LAS X 3.6.1
LAS X 3.6.1 and LAS AF 2.x cannot be installed on the same computer, unless it isconfigured with a dual boot option. Before installation of LAS X 3.6.1 on an offline
workstation running LAS AF 2.x, it is necessary to uninstall LAS AF 2.x.
LAS X 3.6.1 does not accept the LAS AF 2.x license dongle and vice versa. Please
make sure that the correct dongle is inserted in the workstation. LAS X dongles havea Leica logo engraved and a shiny surface to distinguish them from LAS AF 2.x
dongles.
LAS X 3.6.1 Release Notes for Widefield Systems

8

LAS X 3.6.1 can read and process files generated with LAS AF 2.x versions and LAS
AF 4.x versions. LAS AF 2.x versions however cannot read all data acquired with LAS
AF 4 or LAS X software versions.

2.1.4 Compatibility to Leica LMD Laser Microdissection
LAS X is fully compatible to Leica LMD application software and can be installed on
the same workstation.
Important note: Use only one software at a time. The LMD application software and
LAS X software cannot be used simultaneously.
LMD application software V8.x supports Win7 und Win10.

LAS X 3.6.1 Release Notes for Widefield Systems

9

2.2

Installing the Correct Firmware Versions

In most cases, the LAS X installation takes care of installing the required microscope
firmware. However, in some cases, the updating of the firmware may not take place. The
firmware then must be updated manually via the LAS X Hardware Configurator.
The correct versions of firmware for use with LAS X 3.6.1 are:

DMI 8:DMI8 Master (XE167FH200F100L_MASTER.HEX)DMI8 Master FPGA (XP2_17_MASTER.HEX)
SBM I2C (DSPIC33FJ128_SBM_I2C.HEX)
motCORR (DSPIC33FJ128_MOT_CORR.HEX)
AFC (TMS320F28335_AFC.HEX)
SmartMove (TMS320F28023_SMARTMOVE.HEX)
Sideports (DSPIC33FJ128_DC_TURRETII.HEX)
Bottomport (DSPIC33FJ128_DC_TURRETII.HEX)
Z Axis (DSPIC24HJ064_STEPPERA.HEX)XY Axes (DSPIC24HJ064_STEPPERA.HEX)
Buttons Left (DSPIC33FJ128_BUTTONS_LEFT.HEX)
Buttons Right (DSPIC33FJ128_BUTTONS_RIGHT.HEX)
Nosepiece 2 positions
Nosepiece (DSPIC33FJ128_NOSEPIECE.HEX)IL Turret (DSPIC33FJ128_DC_TURRETII.HEX)
Mag. Changer (DSPIC33FJ128_DC_TURRETII.HEX)
T-House Splitter (DSPIC33FJ128_DC_TURRETII_B.HEX)
DIC (DSPIC33FJ128_STEPPERB.HEX)IL Diaphragms (DSPIC33FJ128_STEPPERB.HEX)PIC GSD (PIC_GIST.HEX)
FRAP (DSPIC24HJ064_STEPPERA.HEX)
Lamphouse (STM32F301K8_LAMP2.HEX)WF Scanner
Sequencer 2
Sequencer 2 FPGA
Touch Panel 2nd Generation (TPC-G2.exe)

LAS X 3.6.1 Release Notes for Widefield Systems

V02.61.9116
V210
V01.01
V01.08
V02.21
V01.10
V01.06
V01.06
V01.15
V01.15
V01.08
V01.04
V01.01
V01.14
V01.06
V01.06
V01.05
V01.05
V01.05
V01.01
V01.15
V01.01.9999
V01.15
V01.02
V01.242.14.0.9116

10

Compound (except DMi8):
Master Module(DM4000 ,DM4500, DM5000) (MAN1.HEX)
Leica Screen Module (DM5000) (MAN2.HEX)
Master Module (DM5000, DM5500, DM6000, DM6) (SYS.HEX)
Master Module (DM4000, DM4500, DM4) (BM-16Bit.HEX)
Master Module (DMI6000, DMI5000, DMI4000) (DMI.HEX)
Master Module (DM8000, DM12000) (DM8_12000.HEX)
Touch Panel 2nd Generation (TPC-G2.exe)
Touch Panel (STP6000) (TPC6000.exe)
DM3000 Master (DM3000.HEX)
Condenser Module (PH/DIC) (KONDSCH.HEX)
Condenser Module II (PH/DIC) (KONDSCH01.HEX)
Condenser Module III (PH/DIC) (KONDSCH02.HEX)XYZ Module (DM6000, DM5500) (XYZ_DIS.HEX)
Advanced XYZ Module (XYZ_ADV.HEX)
Advanced Z Module (Z_ADV.HEX)
LMD 6000 Head (LMD_6000.HEX)
Master Module LED4000 (LED4_7000.HEX)
LED7000 (LED7000.HEX)AFC DSP (AFC_DSP.HEX)
PIC motCORR (PIC_Corr.HEX)PIC GSD (PIC_GIST.HEX)

Stereos:
Zoom
Filter Wheel
Focus
Focus DCI (Dragon Focus)
Smart Touch (TCU)
TL RCI BaseTL LED (TL 5000 Ergo B.)
TL 3000 Ergo Base
IsoPro Stage (6x4”)
IsoPro DCI
Obj. revolver
Footswitch

M205FA
4.00.511469
3.03.481710
3.02.492813
4.01.438861
5.00.497063
4.01.338410
4.01.438080
5.00.446074
3.01.167675
3.01.133449
4.00.320268
3.03.255036

LAS X 3.6.1 Release Notes for Widefield Systems

M165 FC
4.00.511469
3.01.120368
3.02.492813
4.01.438861
5.00.497063
4.01.338410
4.01.438080
5.00.446074
3.01.167675
3.01.133449
4.00.320268
3.03.255036

V01.31
V01.07
V02.40.9116
V02.70.9116
V02.80.9116
V02.10.9116
2.14.0.9116
1.46.0.9116
V02.30.9116
V01.06
V01.01
V01.03
V01.10V03.10.9116
V03.10.9116
V01.24.9116
V01.04.9116
V01.04.9116
V01.25
V01.03
V01.01

MacroFluo
4.01.281758--3.02.492813
4.01.438861
5.00.497063
4.01.338410--3.01.167675
3.01.133449--3.03.255036

11

2.3

PC Requirements

2.3.1 PC Requirements
Recommended PC Configuration:
Operating System:

Win10 64bit

CPU:Intel XEONW-2123 3.6 4C CPU

RAM:

64GB DDR4 2666 ECC REG RAM

1st hard drive:

512 GB SSD

2nd hard drive:

4TB 7200 SATA ENT 3.5 2ND HDD

Graphics board:

NVIDIA QUADRO P1000 4GB

High-speed triggered sCMOS cameras or high-speed Navigator
experiments require a SSD RAID NVMe hard disk.

Minimum requirements for EM Cryo CLEM 3 systems:
Operating System:

Win10 64bit

CPU:Intel XeonW-2123 3.6 4C

RAM:

128 GB DDR4-2666 ECC REG

1st hard drive:

SSD 512 GB SATA

2nd hard drive:

2TB SSD RAID NVMe hard disk

Graphics board:

NVIDIA Quadro P1000 (4 GB)

Monitor:minimum screen resolution is 1000 lines vertically

PC minimum requirements (except EM Cryo CLEM 3)
Operating System:

Win7 64bit

CPU:Intel Core-I5 3,20 GHz

RAM:

16 GB DDR3-1333 ECC

Hard drive:

500 GB HDD

Graphics board:

NVIDIA Quadro K600 (1GB)LAS X 3.6.1 Release Notes for Widefield Systems

12

3. Product Security Fix
Update of license component to a new secure version
Please see the official communication for detailed information
https://www.leica-microsystems.com/company/product-security/product-security-updates/

4. Important Information
High-speed triggered sCMOS cameras or high-speed Navigator experiments require thetemp file being located on a SSD RAID NVMe hard disk. Temp file on a normal SSD disk
may cause the experiment to stop without being completed.

"Data handling in folders" requires more space on the temp drive compared to working in
projects. For large experiments it is recommended to use "Data handling in projects".

Experiments with high-speed autofocus and camera in global reset require theexperiment to start with a selected acquisition channel. Starting the experiment with aselected autofocus channel can lead to underexposed images.

Particle tracking and "Analyze for all time points" not working in 2D Analysis. However,particle tracking and analysis over time is working fine in 3D analysis.



EM Cryo CLEM: DFC9000 GT is the tested and approved camera for the EM Cryo
CLEM. No other camera should be used.

When installing LAS X from USB-Stick, Trend Micro blocks the AUTORUN.inf.
Workaround: start setup.exe manually.

When using USB cameras: please disable "USB selective suspend setting" in Windows
advanced power settings. LAS X workstations have these settings already preset.

LAS X 3.6.1 Release Notes for Widefield Systems

13



Photometrics Evolve: brightness of images between live and high-speed acquisition may
differ.



Leica DFC550 and 3d party cameras (various models from Andor, Hamamatsu, PCO):
no different binning values for live and acquired images possible.



HDR acquisition with camera: only 8 bit, high-speed acquisition not supported

If you use a multicolor lightsource on your system, you've to set the IL shutter mode in
the hardware configurator to “Always open after changing contrasting method”.
Otherwise the hardware shutter of the fluo axis stays closed.



Sutter DG4/DG5 – New versions of this light source, which have changed from RS232
connection to USB connection, are not supported in LAS X.

Specimen overview is not offered anymore in LAS X Standard UI. Overviews are now
generated within the LAS X Navigator.



LAS X Navigator is not available within the Easy Operation mode.



Use Do not use Navigator in combination with data handling "Work in Folders". Navigator
experiments require data handling "Work in Projects” (Configuration tab -> User
Configuration -> Data handling -> "Work in Projects")



Do not stop an experiment with remaining manipulation steps in the Scan Sequencer. If
stopped, the remaining manipulation steps cannot be deleted from the Infinity Scanners
memory. All subsequent manipulations will trigger the remaining manipulations andnot the actual definition.

LAS X 3.6.1 Release Notes for Widefield Systems

14The scan sequencer works with AFC in continuous mode but not with the image based
autofocus (HSAF).



If LAS X is to be installed on the same workstation as the LMD application software,
install first the LMD application software and then the LAS X Software afterwards.
Important note: Use only one software at a time. The LMD application software and LAS
X software cannot be used simultaneously.



Huygens Deconvolution: Export of experiments from LAS X to Huygens can fail whenusing older Huygens versions.
Workaround: Please make sure Huygens version 15.10. or above is installed.



If an objective is changed via LAS X Inplace Configuration it can happen that in the AFC
Panel the AFC cannot be set to “HOLD FOCUS POSITION” or “USE AFC”. The
checkbox might be grayed out.
Workaround: Close LAS X and use the HW configurator to teach in the objectives again
followed by power cycle the microscope. Then start the LAS X again.

Image intensities in the live image of a high-speed autofocus channel can differ from theintensities of the high-speed autofocus channel during an experiment. Workaround: use
integer numbers for exposure time in the high-speed autofocus channel, e.g. 4,0msec
instead of 3,9msec.

Advanced Sequencer with TL and IL lamps controlled via I2C connection: image can beilluminated incorrectly. Workaround: use a real TTL Trigger signal to control the shutter.



After acquiring an image with FLUO-PH or FLUO-DIC the illumination panel disappears.
Workaround: switch to a different contrasting method (not FLUO-PH or FLUO-DIC).



Image export: Do not delete experiment data during export of the experiment. Otherwise
the exported data will be corrupted.



3D Surface Viewer is not supported in LASX 3.6.1

Live stream recording: with Windows 7 and MP4, max resolution is 1920x1088 pixel

If you teach in a new TIRF cube or replace an existing one with another type in the
Hardware Configurator, please do not forget to fine-tune afterwards. Otherwise, thesoftware may use incorrect TIRF fine-tune information.



Z-stack acquisition with finefocus in combinaiton with "AFC on demand" does not work.

Restriction for DMI series with manual transmitted light arm: the light intensity of atransmitted light channel can drop to ZERO when changing between channels in the lightpath settings interface. This restriction does not apply to DMi8.
Workaround: keep live image always on when changing between channels

LAS X 3.6.1 Release Notes for Widefield Systems

15



Stereomicroscopes: Measurement function in live mode works incorrectly after an
objective change. Please leave and re-enter the live mode to readjust the measurement
function.



Mobile Connection: iPads require iOS10 to connect with LAS X.

LAS X is released for use with Windows Defender Antivirus and TendMicro Antivirus.

User Management is renamed to Access Management

LAS X 3.6.1 Release Notes for Widefield Systems

16