HIRANUMA APPLICATION DATA

Data No.

Automatic Titrator

K9May 10,2021

Fractional determination of hydrogen peroxideand peracetic acid

Organic acid
1. Abstract

Peracetic acid (CH3COOOH) is used as oxidizing agent, bleaching agent, and fungicide. Peracetic acidgenerates acetic acid and oxygen by its decomposition. It is suggested that the bleaching and antiseptic
properties are derived from this generated oxygen. On the other hand, hydrogen peroxide (H2O2) works as bothoxidizing and reducing agents depending on the target chemical to be reacted. It is used as fungicide in foodindustry as well as peracetic acid.
Recently, the usage of the mixed solution containing hydrogen peroxide, peracetic acid, and acetic acidhas been increasing for the sterilization of beverage containers along with the popularization of a plastic bottle
as containers. This report introduces an example of fractional determination of hydrogen peroxide and peracetic
acid in the mixed solution containing hydrogen peroxide, peracetic acid, and acetic acid.
The fractional titration of hydrogen peroxide and peracetic is performed as follows: first, hydrogen
peroxide is titrated with potassium permanganate standard solution (formula (1)) after the sample solution isacidified with sulfuric acid. After the titration, add potassium iodide to generate iodine equivalent to peraceticacid (formula (2)). This generated iodine is titrated with sodium thiosulfate standard solution to determine theperacetic acid (formula (3)).

2MnO4- + 5H2O2
CH3COOOH
I2+ 2I+ 2Na2S2O3+ 6H+－→2Mn2+ + 5O2(↑)+ 2H+ → I2

→ 2NaI+ H2O+ 8H2O・・・(1)+ CH3COOH・・・(2)+ Na2S4O6・・・(3)2. Configuration of instruments and reagents(1) Configuration
Main unit ：Option：

Automatic Titrator COM series
One buretElectrode ：Platinum electrodePT-301：

Reference electrodeRE-201Z* PR-701BZ platinum reference electrode can also be used for measurement instead ofabove electrodes.
(2) Reagents
Titrant：

0.02 mol/L Potassium permanganate standard solution
0.1 mol/L Sodium thiosulfate standard solution

Additive ：Diluted sulfuric acid (Sulfuric acid : DI water = 1 : 9 [v/v])
1 mol/L Potassium iodide solution

3. Measurement procedure。
(1) Take 2 mL of sample into a 100 mL beaker with a volumetric pipette.
(2) Add about 50 mL of DI water.
(3) Add 2 mL of diluted sulfuric acid.

(4) Immerse the electrodes to start titration. The titration with 0.02 mol/L potassium permanganate standard
solution for hydrogen peroxide is performed.
(5) After the titration of hydrogen peroxide is completed, the waiting time of 120 seconds is automaticallycounted according to the S-Timer setting. During this waiting time, add 2 mL of 1 mol/L potassium iodide
solution using a micropipette.
(6) After the S-Timer is finished, the titration with 0.1 mol/L sodium thiosulfate standard solution forperacetic acid are sequentially performed.

4. Measurement conditions and results
Examples of titration conditions(1) Titration for hydrogen peroxide with potassium permanganate standard solution
Cndt No.
MethodBuret No.
Amp No.
D. Unit
S-Timer
C.P. mLT Timer
D.P. mLEnd Sens
Over mLMax Vol.

1Auto
12mV
10
000.3
500
020sec
mLsec
mL
mL
mL

Constant No.
1Size
2Blank0
Molarity
0.02
Factor
1.003
K85
L0Unit%Formula(D-B)*K*F*M/(S*10)
Decimal places
3
Auto input parametermLmLmol/L

Mode No.
Pre Int
Del K
Del Sens
Int Time
Int Sens
Brt SpeedPulse

505033240

Mode No.
Pre Int
Del K
Del Sens
Int Time
Int Sens
Brt SpeedPulse

505033240sec
mV
sec
mV

None

(2) Titration for peracetic acid with sodium thiosulfate standard solution
Cndt No.
MethodBuret No.
Amp No.
D. Unit
S-Timer
C.P. mLT Timer
D.P. mLEnd Sens
Over mLMax Vol.

3Auto
32mV
120000300
0.220sec
mL
sec
mL
mL
mL

Constant No.
3Size
2Blank
0Molarity0.1
Factor
1.005
K38.03
L1Unit%Formula(D-OA*L)*K*F*M/(S*10)
Decimal places
4
Auto input parameter

mLmLmol/LsecmVsecmV

*1

None
*1”OA” and “L” in the formula
The excess potassium permanganate after the titration for hydrogen peroxide reacts with potassium iodide and generates iodine,
therefore it must be subtracted. The “OA” in the formula means excess titrated volume of potassium permanganate, theconcentration ratio of normality for potassium permanganate standard solution and sodium thiosulfate standard solution is enteredto L (Coefficient 2). (“1” is entered on this report because normality of both reagents are 0.1 N.). The excess potassium
permanganate is subtracted with this formula.

Measurement results
Measurement results of hydrogen peroxide
Number ofSize

Titrant

Hydrogen peroxide

Measurement(mL)

Volume (mL)Concentration (%)

12

13.2191.127

2213.217

1.127

3213.265

1.131

Statistic
calculation

Average

1.128 %

Standard deviation

0.0023 %Coefficient of variation

0.205 %

Titration curve for hydrogen peroxide

Measurement results of peracetic acid
Number ofSize

Titrant

Peracetic acidMeasurement(mL)

Volume (mL)Concentration (%)

122.4140.436322

2.378

0.4290322.4160.4359Statistic
calculation

Average0.434 %

Standard deviation

0.0041 %Coefficient of variation

0.946 %

Titration curve for peracetic acid

Example of titration curve

5. NoteHydrogen peroxide and peracetic acid were determined by redox titration. The addition of optional one
buret allows to perform fractional titration for hydrogen peroxide and peracetic acid as described in this report. If
required, add another optional buret for potassium iodide solution for more automation. However, the potassium
iodide solution tends to precipitate, causing clogging of the flow path of buret. Therefore, it is necessary toremove the easily clogged buret tip from the buret for potassium iodide solution, and to clean the flow path withpure water after daily measurement.
Keywords: Hydrogen peroxide, Peracetic acid, Potassium permanganate, Potassium iodide, Sodium thiosulfate,Redox titration*Some measurement would not be possible depending on optional configuration of system.