&

MICROBES AND ENVIRONMENTSEDITORIAL BOARD (2013-2014)

Editor-in-Chief

Yoichi Kamagata, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan

Senior Editors

Masahito Hayatsu, National Institute for Agro-Environmental Sciences, Tsukuba, Japan
Koki Toyota, Tokyo University of Agriculture and Technology, Koganei, Japan
Shin Haruta, Tokyo Metropolitan University, Hachioji, Japan
Kazuya Watanabe, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan

Managing Editors

Kumiko Kita-Tsukamoto (Manuscript Reception), The University of Tokyo, Kashiwa, Japan
Shigeto Otsuka (Online Journal), The University of Tokyo, Tokyo, Japan
Takashi Yoshida (Online Journal), Kyoto University, Kyoto, Japan
Tomoo Sawabe (Online Journal), Hokkaido University, Hakodate, Japan
Seigo Amachi (Manuscript Edition), Chiba University, Matsudo, Japan
Takashi Narihiro (Manuscript Edition), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan
Satoshi Hattori (Manuscript Edition), Yamagata University, Tsuruoka, Japan
Koji Mori (Manuscript Edition), NITE Biological Resource Center (NBRC), Kisarazu, Japan
Yoshinori Sato (Manuscript Edition), National Institutes for Cultural Heritage, Tokyo, Japan
Satoshi Ishii (Manuscript Edition), Hokkaido University, Sapporo, Japan
Yuko Shinohara (Manuscript Edition), Junior College of Kagawa Nutrition University, Tokyo, Japan
Satoko Noda (Japanese Series), Yamanashi University, Kofu, Japan
Naoko Yoshida (Japanese Series), Toyohashi University of Technology, Toyohashi, Japan
Yasuhiro Kasahara (Japanese Series), Hokkaido University, Sapporo, Japan

Associate Editors

Chih-Yu Chiu, Academia Sinica, Taipei, Taiwan
Jongsik Chun, Seoul National University, Seoul, Korea
Jang-Cheon Cho, Inha University, Incheon, Korea
Wentso Liu, University of Illinois at Urbana-Champaign, Champaign, IL, USA
Hiromitsu Furuya, Akita Prefectural University, Akita, Japan
Hiroyuki Futamata, Shizuoka University, Hamamatsu, Japan
Natsuko Hamamura, Ehime University, Matsuyama, Japan
Satoshi Hanada, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan
Akira Hiraishi, Toyohashi University of Technology, Toyohashi, Japan
Shir-Ly Huang, National Central University, Jhongli City, Taiwan
Elena P. Ivanova, Swinburne University of Technology, Melbourne, Australia
Junichi Kato, Hiroshima University, Higashi-Hiroshima, JapanBon Kimura, Tokyo University of Marine Science and Technology, Tokyo, Japan
Staffan Kjelleberg, The University of New South Wales, Sydney, Australia
Yukari Kuga, Hiroshima University, Higashi-Hiroshima, JapanKen Kurokawa, Tokyo Institute of Technology, Yokohama, Japan
Mei-Chin Lai, National Chung Hsing University, Taichung, Taiwan
Shuang-Jiang Liu, Institute of Microbiology Chinese Academy of Sciences (IMCAS), Beijing, China
Fumito Maruyama, Tokyo Medical and Dental University, Tokyo, Japan
Kiwamu Minamisawa, Tohoku University, Sendai, Japan
Keisuke Miyauchi, Tohoku Gakuin University, Tagajo, Japan
Hiroharu Murakami, NARO Western Region Agricultural Research Center, Ayabe, Japan
Jun Murase, Nagoya University, Nagoya, Japan
Shin-ichi Nakano, Kyoto University, Otsu, Japan
Toshi Nagata, The University of Tokyo, Kashiwa, Japan
Masaya Nishiyama, Nagasaki University, Nagasaki, Japan
Hideaki Nojiri, The University of Tokyo, Tokyo, Japan
Moriya Ohkuma, RIKEN BioResource Center, Wako, Japan
Hiroyuki Ohta, Ibaraki University, Ami, Japan
Joonhong Park, Yonsei University, Seoul, Korea
Michael J. Sadowsky, University of Minnesota, St. Paul, MN, USA
Akihiro Saito, Shizuoka Institute of Science and Technology, Fukuroi, Japan
Masanori Saito, Tohoku University, Osaki, Japan
Keishi Senoo, The University of Tokyo, Tokyo, Japan
Toru Shigematsu, Niigata University of Pharmacy and Applied Sciences, Niigata, Japan
Masahiro Shishido, Chiba University, Matsudo, Japan
Yuichi Suwa, Chuo University, Tokyo, Japan
Satoru Suzuki, Ehime University, Matsuyama, JapanKen Takai, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
Hideto Takami, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan
Sen-Lin Tang, Academia Sinica, Taipei, Taiwan
Shigenobu Yoshida, National Institute for Agro-Environmental Sciences, Tsukuba, Japan
Lian-Hui Zhang, Institute of Molecular and Cell Biology, Proteos, Singapore

JAPANESE SOCIETY OF MICROBIAL ECOLOGY

President (2013–2014): Kiwamu MINAMISAWA, Tohoku University
Secretary (2013–2014): Keishi SENOO, The University of Tokyo
Business affairs (2013–2014): Shigeto OTSUKA, The University of Tokyo
Business affairs (2014): Kazuo ISOBE, The University of Tokyo
Treasurer (2013–2014): Futoshi KURISU, The University of Tokyo
International affairs (2013–2014): Kazuhiro KOGURE, The University of Tokyo
Public relations (2013–2014): Michinari SUNAMURA, The Univerisyt of Tokyo
Office of the Society (Journal subscription request should be directed to): Keishi SENOOLaboratory of Soil Sciences, Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku,
Tokyo 113-8657, Japan
Tel/Fax: +81–3–5841–5139/8042 E-mail: jsme.office@gmail.com

JAPANESE SOCIETY OF SOIL MICROBIOLOGY

President (2013-2015): Masanori SAITO, Tohoku University
Secretary (2014-2015): Tomoyoshi HASHIMOTO, NARO Agricultural Research Center
Treasurer (2014-2015): Kazunari NAGAOKA, NARO Agricultural Research Center

TAIWAN SOCIETY OF MICROBIAL ECOLOGY

President (2010-2014): Shiu-Mei LIU, National Taiwan Ocean University
Vice President (2010-2014): Shir-Ly HUANG, National Central University
Secretary (2012-2014): Hong-Thih LAI, National Chiayi University

This journal is partly subsidized by the Grant-in-Aid for Publication of Scientific Research Results from the Japan Society for the Promotion of Science(JSPS).

c/o NakaNishi PriNtiNg Co.
Shimodachiuri Ogawa Higashi Kamikyoku, Kyoto 602-8048, Japan TEL: +81–75–441–3155, FAX: +81–75–417–2050

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

Microbes and Environments
Instructions for Authors
Microbes and Environments (M&E) is an international journal devoted tothe publication of original and minireview articles regarding all aspects ofresearch on microbial ecology and environmental microbiology. Articles candeal with culturable and non-culturable forms of any type of microorganism:
Bacteria, archaea, fungi, yeasts, protozoa, algae and viruses. M&E is an
open access journal (for online articles), published by Japanese Society ofMicrobial Ecology (JSME), Japanese Society of Soil Microbiology (JSSM),
and Taiwan Society of Microbial Ecology (TSME).
1. Types of papers published
Four types of papers are published: Full-length Regular Papers, Short
Communications, Reviews, and Short Reviews. Regular Papers and Short
Communications must represent a report of original research thathas not been published before (except in the form of an abstract or as partof a published lecture, review, or thesis) and is not under consideration
for publication elsewhere. Reviews are normally invited. Authorsinterested in submitting a Review should write to the Editor-in-Chief(E-mail: y.kamagata@aist.go.jp) for approval before its submission. Short
Reviews are completely invited.
2. Copyright and agreement for publication
All authors of a manuscript must have agreed to its submission and are
responsible for its contents, including appropriate citations and acknowledgements. All authors also must have agreed that the corresponding author
has authority to act on their behalf in all matters pertaining to publication of
the manuscript. Unless the Copyright Transfer Agreement is executed, M&E
will not publish the article. The author guarantees that the manuscript will
not be published elsewhere in any other language without the written permission of the copyright holders. Copyright for supplemental material
remains with the authors, but a license permitting posting by M&E will besent, along with the article copyright transfer agreement, to the corresponding author for signing at the acceptance stage.
3. Submission procedure
All manuscripts must be submitted electronically via the online manu­
script submission and peer review system at the following URL: http://
mc.manuscriptcentral.com/jsme2 (E­mail submissions will not be
accepted). A user ID and password are required and can be obtained on
the first use of the system. Most of file types are supported, but the following
types are recommended: main document in Microsoft Word or generic richtext format (RTF), figures in high resolution EPS or TIFF. PDF file formain document is not acceptable. Total file size (main document, figures,tables, and supplemental materials) should not be exceeded 20 MB. Stepby-step instructions for submitting a manuscript via the online submission
system are available. Authors in Japan should give their address in Japanese
as well as in English. The Editorial Office will confirm the editorial style ofa submitted manuscript. Authors who are unsure of proper English usage
should have their manuscripts checked by someone proficient in the English
language.
4. Review process
Submitted manuscripts are sent to a Senior Editor with appropriate expertise,
who is responsible for making a final decision on its acceptability. The
Senior Editor will choose an Associate Editor who is in charge of sending
the manuscript to two referees for criticism and further communication withauthors. A manuscript returned to the author for revision should be resubmitted to the Associate Editor within 6 weeks. Otherwise it may be considered
withdrawn or treated as a new submission.
5. Accepted manuscripts
When the manuscript has been accepted, the Managing Editor will correct
the style and the format of the manuscript. The corresponding author will
receive a galley proof for one revision. If the galley proof is not returned
before the deadline, the paper may be printed without corrections.
6. Advance Publication online
M&E posts online PDF file versions of manuscripts that have been peer
reviewed and accepted but are being copyedited. Advance publication
manuscripts are accessible from the M&E website. No corrections/changes
to the PDF manuscripts are accepted. Accordingly, authors may find differences between M&E advance publication manuscripts and the final, typeset
articles. Supplemental material for publication is not posted until the final,
typeset article.
7. Preparation of manuscripts
Manuscripts should be written in clear and simple English and be doublespaced for all text on A4 size sheets. All pages must be numbered con­secutively, starting with the title page. The lines of the text should benumbered to aid the reviewers. The arrangement of manuscripts forRegular Papers should be in the following order: Title, Author’s name andAddress, Abstract, Key words (no more than 5 words), Introduction, Materials
and Methods, Results, Discussion (or Results and Discussion), Acknowledgements, References, Tables, Figure legends and Figures. Use section headings (e.g. Introduction, Materials and Methods etc.) in the main text. The
Abstract should not exceed 250 words for a Regular Paper, and 100
words for a Short Communication.
Title page: The title page should include the title, the author’s full name,affiliations, address, and the running head. The title should be as concise

and informative as possible. Multiple authors with different addresses must
indicate their respective addresses with numerals. Place an asterisk after
the name of the author to whom correspondence regarding the manuscript
should be addressed. The abbreviated running head must not exceed 40
characters.
Main document: Subheadings can be used in the Materials and Methods andResults sections of Regular Papers. Use of footnotes is not recommended,
but abbreviations, when necessary, can be defined in a single footnote
inserted in the first page of the text.
References: Arrange the citations in alphabetical order by first author, andnumber consecutively. The references should not include personal communications, unpublished data or manuscripts in preparation (can be mentioned
parenthetically in the text). All listed references must be cited parentheticallyby number in the main text. Journal names should be abbreviated according
to the Chemical Abstracts Service Source Index (CASSI). Each citationmust be complete: Do not use ibid., idem., etc. Articles written by more than
10 authors should be edited to show the first three authors, and ‘et al’.
Acceptable styles are shown in the examples below. For additional styles
and formats, consult recent issues of M&E.
1. Saito, A., S. Ikeda, H. Ezura, and K. Minamisawa. 2007. Microbialcommunity analysis of the phytosphere using culture-independent methodologies. Microbes Environ. 22:93–105.
2. Katsuyama, C., N. Kondo, Y. Suwa, T. Yamagishi, M. Itoh, N. Ohte, H.
Kimura, K. Nagaosa, and K. Kato. 11 November 2008. Denitrification activity and relevant bacteria revealed by nitrite reductase gene fragments in soilof temperate mixed forest. Microbes Environ. doi:10.1264/jsme2.ME08541.
3. Sambrook, J., and D.W. Russell. 2001. Molecular Cloning: A Laboratory
Manual, 3rd ed. Cold Spring Harbor Laboratory Press, New York.
4. Hiraishi, A. 1989. Isoprenoid quinone profiles for identifying and classifying microorganisms in the environment, p. 663-668. In T. Hattori, Y.
Ishida, Y. Maruyama, R. Y. Morita, and A. Uchida (ed.), Recent
Advances in Microbial Ecology. Japan Scientific Societies Press, Tokyo.
Figures: All figures including drawings and photographs should be of
their intended publication size, and numbered consecutively. Indicate thefigure numbers and first-author’s name in the margin. Titles and legends offigures must be typed separately. Graphs should be made from finisheddrawings not requiring additional artwork or typesetting. Photographs
should be glossy and rich in contrast. Give scales in microphotographs: Do
not report enlargement factors in legends. Figures in 1-column (maximum
width ca. 8.3 cm) of the printed page are strongly recommended. The maximum width for a 2-column figure should be 17.6 cm.
Tables: Each table must be typed on a separate page, and all tables shouldhave titles. Abbreviations, when necessary, should be defined in table footnotes. The maximum width of a table on the printed page should be 17.6 cm,
and thus the author should prepare tables as concise as possible.
Supplemental material: Supplemental material may be posted by M&E for
any types of paper. M&E encourages that supplemental material be uploadedin the online submission system as a single PDF file or a single video imaging file. The maximum size permitted for an individual file is 10 MB.
Supplemental figures and tables should be referred to as Fig. S1 and Table
S1, respectively. A figure for large phylogenetic tree should be basicallysupplied as a supplemental material.
8. Page Charges
Starting January 1st, 2013, authors will be required to pay page charges
for accepted manuscripts submitted after the start date. Instead,authors will NOT be charged for English proofreading. For a corre­
sponding author who is a JSME/JSSM/TSME member, page charges
are currently ¥4,000 per page. For a nonmember corresponding author,
page charges are currently ¥8,000 per page. Color pages will be printed
at the author’s expense. The cost of publishing colored figures needs tobe borne by the authors. The costs are ¥50,000 for each printed color
page. The authors may choose a lower-cost color page only for publicationin the online version of M&E. In this publication style, the page in theprinted journal is in black and white, however the web page of the online
journal is in full-color.
9. Permission
The corresponding author is responsible for obtaining permission from boththe original author(s) and the publishers (i.e., the copyright owner) to reproduce or modify figures and tables, and to reproduce text from previous publications. Statements indicating that the material is being reprinted with permission must be included in the relevant figure legend or table footnote of
the manuscript. Reprinted text must be enclosed in quotation marks, andthe permission statement must be included as running text or indicatedparenthetically.
The latest full version of Instructions for Authors is also available throughthe Internet at the following URL: http://www.microbial-ecology.jp/files/
user/Instructions_full_latest.pdf
Copyright (C) 2014, Japanese Society of Microbial Ecology (JSME) /Japanese Society of Soil Microbiology (JSSM) / Taiwan Society of Microbial
Ecology (TSME). All rights reserved