1996 TOKYO INTERNATIONALSYMPOSIUM ON POLYAMINESPROGRAM★
ORAL SESSIONS[Opening Address] Oshima, T. (Tokyo University of Pharmacy and Life Science)[Opening Lecture]
Chairperson: Pegg, A. E. (Pennsylvania State University)[01] Hayashi, S. (The Jikei University School of Medicine)
ODC antizyme: a unique regulatory protein and a possible tumor suppressor?
[Session 1. Biosynthesis and Its Regulation]
Chairperson : Mitchell, J. L. A. (Northern Illinois University), Otani, S. (Osaka City
University)[02] Coffino, P. (University of California)
Regulation of ornithine decarboxylase by antizyme.
[03] Heby, O. (Ume・University)
The intronless mouse S-adenosylmethionine decarboxylase gene has acquired astrong and functional promoter.
Chairperson : Coffino, P. (University of California), Matsui-Yuasa, I. (Osaka City
University)[04] Morris, D. R. (University of Washington)
Regulation of S-adenosylmethionine decarboxylase translation in mammals.
[05] Mitchell, J. L. A. (Northern Illinois University)

Antizyme interaction with the stable ornithine decarboxylase of DH23b cells.
[06] Michael, A. (Institute of Food Research)
The plant polyamine pathway.
[Session 2. Polyamine Contents and Their Regulation]
Chairperson : Tabor, H. (NIH), Suzuki, O. (Hamamatsu University School of Medicine)[07] Pegg, A. E. (Pennsylvania State University)
Structure and regulation of spermidine/spermine-N1-acetyltransferase.
[08] Suzuki, O. (Hamamatsu University School of Medicine)
Organic cofactor(s) of amine oxidases.
[09] Grillo, M. A. (University of Torino)
Polyamines in hepatocytes.
[10] Algranati, I. D. (Instituto de Investigaciones Bioqu 知 icas)
Polyamines, control of proliferation and one-step selection of
a-difluoromethylornithine-resistant cell lines in Leishmania mexicana.
[11] Davis, R. H. (University of California)
Polyamine-dependent mechanisms regulating the abundance of ornithine
decarboxylase mRNA in Neurospora crassa.
[Session 3. Functions of Polyamines in Cell Growth]
Chairperson : Chen, K. Y. (The State University of New Jersey), Igarashi, K. (Chiba
University)[12] Park, M. H. (NIH)
The essential role of the polyamine-derived amino acid, hypusine, in eukaryotic
cell proliferation.
[13] Shida, H. (Kyoto University)Cofactors and inhibitor of human immunodeficiency virus Rev and human T-cellleukemia virus type I Rex.
[14] Tabor, C. W. (NIH)
Biosynthesis and function of polyamines in Saccharomyces cerevisiae.
Chairperson : Bachrach, U. (Hebrew University), Kawakita, M. (Tokyo Metropolitan Inst.
Med. Sci.)[15] Shirahata, A. (Josai University)
Spermidine accumulation and cell death of HTC cells pretreated with ODC
inhibitor.
[16] Holtta, E. J. (University of Helsinki)
Ornithine decarboxylase gene - an oncogene?
[17] Bachrach, U. (Hebrew University)
Effect of polyamines on nuclear oncogene expression.
[18] Janne, J. (University of Kuopio)
Genetic engineering of the metabolism of the polyamines in transgenic mice.
[Session 4. Polyamine Interaction with Macromolecules]
Chairperson : Persson, L. (University of Lund), Samejima, K. (Josai University)[19] Oshima, T. (Tokyo Univ. of Pharm. Life Sci.)
Effects of a naturally occurring quaternary polyamine on cell-free protein
biosynthesis catalyzed by a thermophile extract.
[20] Frydman, B. J. (University of Wisconsin)
Spermine binds in solution to the TyC loop of tRNAPhe: evidence from a 750
MHz 1H-NMR analysis.
[21] Igarashi, K. (Chiba University)Structure and function of the PotD protein in the spermidine-preferential uptake
system.
[22] Yamada, M. (Osaka University)
Gating of inwardly rectifying potassium channels by intracellular polyamine.
[23] Williams, K. (University of Pennsylvania)
Modulation and block of ion channels: a new biology of polyamines. Studies
with recombinant NMDA receptors.
[Session 5. Biological Action of Polyamines]
Chairperson : Porter, C. W. (Roswell Park Cancer Institute), Matsuzaki, S. (Dokkyo
University School of Medicine)[24] Otani, S. (Osaka City University)
Involvement of polyamines in the production of cytokine-induced neutrophil
chemoattractant (CINC) from LPS-stimulated rat alveolar macrophages.
[25] Fujiwara, K. (Saitama Medical School)
The role of putrescine in the regulation of liver regeneration.
[26] Matsuzaki, S. (Dokkyo University School of Medicine)Acute inhibition of thyroid ornithine decarboxylase by excess iodine in the rat.
Chairperson : Scalabrino, S. (University of Milano), Fujiwara, K. (Saitama Medical
School)[27] Porter, C. W. (Roswell Park Cancer Institute)
Regulatory responses in polyamine homeostasis.
[28] Nakashima, K. (Mie University)
Key roles of polyamines and ODC induction in pancreas and nerve tissue
regenerations.

[Session 6. Clinical Aspects of Polyamines]
Chairperson : Moulinoux, J.-Ph. (URA CNRS), Nakashima, K. (Mie University)[29] Moulinoux, J.-Ph. (URA CNRS)
Wanted: erythrocytes dead or alive?
[30] Kawakita, M. (Tokyo Metropolitan Inst. Med. Sci.)
N1,N8-Diacetylspermidine and N1,N12-diacetylspermine: detection in humanurine and potential usefulness as novel tumor markers.
[31] Ohsawa, N. (Osaka Medical College)
Clinical significance of the measurement of polyamines in biological fluids.
Chairperson : Marton, L. J. (University of Wisconsin), Kamei, S. (Tokyo Medical and
Dental University)[32] Luk, G. D. (University of Texas)
Ornithine decarboxylase and carcinogenesis - overexpression by a gut-specificpromoter.
[33] Nishioka, K. (University of Texas)
Phase I dose de-escalation study of DFMO as a chemopreventive agent inpatients with cervical intraepithelial neoplasia grade 3 (CIN3).
[Closing Lecture]
Chairperson : Oka, T. (NIH)[34] Marton, L. J. (University of Wisconsin)
Transitions between basic and clinical science.
[Closing Address] Ohsawa, N. (Osaka Medical College)★
POSTER SESSIONS

[Poster Session 1.]
Leader: Luk, G. D. (University of Texas), Kashiwagi, K. (Chiba University)[P01] Apirakaramwong, A. (Chiba University)
Decrease in cell viability in the Escherichia coli mutant
lacking rmf and ompC genes.
[P02] Kashiwagi, K. (Chiba University)
Molecular mechanism of polyamine stimulation of the synthesis of oligo-peptide
binding protein.
[P03] Minchin, R. F. (University of Western Australia)
Studies on the bacterial putrescine transporter encoded by the speF-potE operon.
[P04] Ohe, M. (Dokkyo University School of Medicine)
Effects of polyamines on hydrolysis of adenosine 5'-triphosphate catalyzed
by Escherichia coli GroEL.
[P05] Takatsuka, Y. (Tohoku University)
Purification and characterization of lysine decarboxylase from Selenomonas
ruminantium; the bifunctional enzyme.
[P06] Yamami, T. (Chiba University)
Spermidine dependent expression of spo0KA during sporulation in Bacillus
subtilis.
[P07] Yamamoto, S. (Okayama University)
Characterization of the genes required for 1,3-diaminopropane biosynthesis
from Acinetobacter baumannii.
[P08] Takaji, S. (Mie University)
Growth inhibition of Helicobacter pylori by methylglyoxalbis(cyclopentyl-amidinohydrazone).

[P09] Audette, M. (Chul Research Center)
Functional characterization of STK2, a putative serine/threonine protein kinase
involved in the regulation of high affinity polyamine transport in Saccharomyces
cerevisiae.
[P10] Abid, Md. R. (Nagoya University)
Deoxyhypusine synthase expressed from yeast gene or from human cDNA are
essentially interchangeable when overexpressed in yeast.
[P11] Bagni, N. (University of Bologna)
Polyamine binding to proteins solubilized from plasma membrane.
[P12] Bagni, N. (University of Bologna)
Uptake competition between normal and unusual polyamines in carrot protoplasts.
[P13] Bagni, N. (University of Bologna)
Polyamines in phloem exudate of Ricinus plants.
[P14] Serafini-Fracassini, D. (University of Bologna)
Conjugated polyamines of chloroplasts are involved in salt stress responseof Dunaliella salina.
[P15] Serafini-Fracassini, D. (University of Bologna)
Transglutaminases partially purified from sprout apices of Helianthus tuberosus.
[P16] Serafini-Fracassini, D. (University of Bologna)
Covalently bound polyamines and photosynthesis.
[P17] Serafini-Fracassini, D. (University of Bologna)
Polyamines are conjugated to cytoskeleton proteins during apical growth of thepollen tube.
[P18] Torrigiani, P. (University of Bologna)

Spermidine biosynthesis gene expression in Tobacco during flower development.
[P19] Tiburcio, A. F. (University of Barcelona)
Inducible over-expression of oat arginine decarboxylase in transgenic tobacco
plants.
[P20] Fujihara, S. (Shikoku National Agricultural Experiment Station)
Occurrence of unsaturated polyamines in legume root nodules.
[P21] Minocha, S. (University of New Hampshire)
Genetic manipulation of the polyamine biosynthetic pathway promotes somatic
embryogenesis and stress tolerance in carrot (Daucus carota L.).
[P22] Maki, H. (Japan Women's University)
Polyamines and the cell cycle in synchronous cultures of higher plants(Catharanthus roseus and Oryza sativa) cells.
[P23] Otsuka, Y. (Tottori University)
Polyamine contents in foods measured by pre-column derivatization.
[P24] Udaka, S. (Tokyo University of Agriculture)
Polyamine contents in ordinary foodstuffs and various fermented foods.
[P25] Simon-Sarkadi, L. (Technical University of Budapest)
Polyamines in food.
[P26] Pusztai, A. (The Rowett Research Institute)
Contribution of gut bacteria to systemic polyamine pools in the rat.
[P27] Bardocz, S. (The Rowett Research Institute)
Manipulation of polyamine body pools to improve health.
[P28] Fujiwara, K. (Nagasaki University)

Immunocytochemical localization of polyamines in the gastrointestinal tracts ofrats and mice.
[P29] Hiramatsu, K. (The Tokyo Metropolitan Institute of Medical Sciences)
Preparation and properties of antibodies specific for N1,N8-diacetyl-spermidine
and N1,N12-diacetylspermine.
[P30] Moulinoux, J.-Ph. (URA CNRS)
Erythrocyte spermine level: a prognostic parameter in childhood common acutelymphoblastic leukemia.
[P31] Moulinoux, J.-Ph. (URA CNRS)Pretherapeutic erythrocyte polyamine spermine levels discriminate high risk
relapsing patients with M1 prostate carcinoma.
[P32] Watanabe, S. (Kawasaki Medical School)
Study of polyamine levels in various tissues of 3-hydroxy-4-methoxy-cinnamic
acid- and 3,4-dimethoxycinnamic acid-treated rats for management of cancers.
[P33] Wallace, H. M. (University of Aberdeen)
Changes in polyamine catabolic enzymes explain the increased content ofacetylpolyamines in human breast cancer tissue.
[P34] Wallace, H. M. (University of Aberdeen)
Polyamine regulation of etoposide-induced apoptosis in human promyelo-genous
leukaemic cells (HL60).
[P35] Matsui-Yuasa, I. (Osaka City University)
Mechanism of antitumor effect of tea polyphenols in Ehrlich ascites tumor cells.
[P36] Kramer, D. L. (Roswell Park Cancer Institute)Differential polyamine regulatory and growth responses to novel spermine analogs
in MALME-3M human melanoma cells.

[P37] Beninati, S. (II University of Rome)Transglutaminase-catalyzed polyamine incorporation into fibronectin reduces theinvasiveness of B16-F10 melanoma cells estimated by an in vitro metastatic assay.
[P38] Messina, A. (University of Catania)
The inhibition of interferon regulatory factor 1 in U937 cells causes a decrease in
the expression of the ornithine decarboxylase gene upon interferon gamma activation.
[P39] Schipper, R. G. (University Hospital Nijmegen)Role of polyamines in TNF and aFas-induced apoptosis in different human andmurine tumor cell lines.
[Poster Session 2.]
Leader: Park, M. H. (NIH), Matsufuji, S. (Jikei Univ. Sch. Med.)[P40] Bachmann, A. S. (University of Hawaii)
Phaseolotoxin: a possible new inhibitor of ornithine decarboxylase.
[P41] Miyoshi, K. (The University of Tokushima)
Intracelluar Ca2+ decreases ornithine decarboxylase activity in cultured rat parotid
explants.
[P42] Scalabrino, G. (University of Milano)
Different patterns of expression of ornithine decarboxylase mRNAs in rat liver after acuteadministration of hepatocarcinogens or TGF-beta.
[P43] Sasaki, H. (Mie University)
Role of ornithine decarboxylase activation in regeneration of Schwann cell tube within asilicon chamber.
[P44] Nakatani, T. (Osaka City University)
The requirement of Zn2+ for the increase in ornithine decarboxylase in primary cultured
rat hepatocytes.

[P45] Morita, A. (Mie University)
Involvement of ornithine decarboxylase and DNA synthesis in proliferation ofmyosatellite cells during rat muscle regeneration after denervation.
[P46] Komine, T. (The Jikei University School of Medicine)
Effect of dietary protein on myocardial ornithine decarboxylase activity in the rats.
[P47] Kontusaari, S. I. (University of Oulu)
Expression of ornithine decarboxylase in tissues of transgenic mice carrying an
MMTV/ODC cDNA fusiongene.
[P48] Persson, L. (University of Lund)
Cloning of a trypanosomatid gene coding for an ornithine decarboxylase that is
metabolically unstable even though it lacks the C-terminal degradation domain.
[P49] Holm, I. (Ume・University)
Molecular cloning and sequencing of a gene and a corresponding cDNA encoding mouseornithine decarboxylase antizyme. Expression in mouse cells.
[P50] Hietala, O. A. (University of Oulu)
Distributions of ornithine decarboxylase and antizyme in rat brain.
[P51] Mizutani, S. (The National Children's Medical Research Center)
Anti-tumor activity of ornithine decarboxylase (ODC)-antizyme targeted against ODC
which is required for cell growth and transformation.
[P52] Matsufuji, S. (The Jikei University School of Medicine)
Mammalian antizyme frameshifting in yeasts, Saccharomyces
cerevisiae and Schizosaccharomyces pombe.
[P53] Murakami, Y. (The Jikei University School of Medicine)
Antizyme is not trapped in the 26S proteasome during ODC degradation.

[P54] Sakata, K. (Chiba University)
Identification of regulatory regions of antizyme for polyamine transport.
[P55] Shinga, J. (University of Tokyo)
Specific developmental arrest at the gastrula stage in Xenopus embryos overexpressedwith S-adenosylmethionine decarboxylase.
[P56] Madhubala, R. (Jawaharlal Nehru University)
Inhibition of Leishmania donovani but not host macrophages by apotent S-adenosylmethionine decarboxylase inhibitor.
[P57] Pietila, M. T. (University of Kuopio)
Transgenic mice over-expressing the mouse
spermidine/spermine N1-acetyltransferase-gene.
[P58] Yano, Y. (Osaka City University)
Effects of expression spermidine/spermine N1-acetyltransferase in HLF cells, humanhepatocellular calcinoma cell line.
[P59] Otani, K. (Osaka City University)
Hypertonic NaCl solution induced spermidine/spermine N1-acetyltransferase in rat
gastric mucosa.
[P60] Verhofstad, A. (University Hospital Nijmegen)Localization of polyamine oxidase activity in rat tissues.
[P61] Ferioli, M. E. (CNR)
Effect of acute hormonal treatment on polyamine oxidase activity in rat tissues.
[P62] Poulin, R. (Chul Research Center)
2,2-Dithiobis(N-ethyl-spermine-5-carboxamide): synthesis and properties of a highaffinity, membrane impermeant antagonist of the mammalian polyamine transportsystem.

[P63] Miki, T. (The Tokyo Metropolitan Institute of Medical Sciences)
Uptake of polyamines by polarized porcine renal cells (LLC-PK1).
[P64] Chen, K. Y. (Rutgers - The State University of New Jersey)
Biochemistry and possible function of deoxyhypusine and hypusine formation of
eukaryotic initiation factor 5A.
[P65] Morgan, D. M. L. (King's college London)
Structural requirements for substrate binding to the polyamine carrier(s) of humanvascular endothelial cells.
[P66] Shimogori, T (Chiba University)
Spermidine regulation of protein synthesis at the level of initiation complex formation of
Met-tRNAi, mRNA and ribosomes.
[P67] Thomas, T. J. (UMDNJ-Robert Wood Johnson Medical School)
Selective utilization of polyamine analogues as a novel strategy for triplex DNA-based
anti-gene therapeutics.
[P68] Weiger, T. (University of Salzburg)
Polyamines modulate calcium activated potassium channels.
[P69] Shirahata, A. (Josai University)
Synthetic diacetylpolyamine substrates for rat polyamine oxidase.
[P70] Penafiel, R. (University of Murcia)
Influence of citrulline and arginine on polyamine metabolism in the mouse kidney.
[P71] Oredsson, S. M. (Lund University)
Normal G1/S transition and prolonged S phase within one cell cycle in Chinese hamster
ovary cells seeded in the presence of polyamine biosynthesis inhibitors.
[P72] Cho, S.-C. (Fujita Health University)Changes of polyamine contents on cultured mammalian cells in the early incubation stage
after X-irradiation.
[P73] Deloyer, P. (University of Liege)
Effect of prolonged spermine treatment on intestinal maturation in the suckling rat.
[P74] Ichimura, S. (National Institute of Radiological Science)
The decrease in the putrescine level of HeLa cells prior to the depression of growth by
X-ray, C-ion beam and H2O2 treatments.
[P75] Mizokami, T. (The Jikei University School of Medicine)
Effects of anti-hypertensive agents on cardiac polyamine contents of spontaneously
hypertensive rats.
[P76] Myojo, S. (Shiga University of Medical Science)
The effect of glicentin on polyamine metabolism in the small intestinal mucosa of rats, invitro and in vivo.
[P77] Sasaki, H. (The Jikei University School of Medicine)
Effects of angiotensin I converting enzyme inhibitor and b-adrenergic blocking agents on
isoproterenol-induced cardiac hypertrophy and cardiac polyamine contents.
[P78] Wu, C. (Osaka City University)
Polyamine in the protection of taurine against the cytotoxicity of hydrazine or carbon
tetrachloride in isolated rat hepatocytes.
[P79] Tsujikawa, T. (Shiga University of Medical Science)
Characteristic of mucosal polyamine content at rectum in patients with ulcerative colitis.
[P80] Delcros, J.-G. (URA CNRS)Polyamine content and uptake in red blood cells from rats grafted with the MAT-LyLu
prostatic adenocarcinoma.
[P81] Delcros, J.-G. (URA CNRS)Polyamine deprivation provokes an antalgic effect.