2010 INTERNATIONAL POLYAMINE CONFERENCE
Progress in Medicine and Life Sciences

June 14 (Mon) -18 (Fri), 2010
Gotemba Kogen Hotel BU at Gotemba Kogen Resort“Toki-no-sumika”

TIME TABLE
MondayJune 14

Tuesday
June 15

WednesdayJune 16

ThursdayJune 17

FridayJune 18

6:30-8:45
Breakfast

6:30-8:45
Breakfast

6:30-8:30
Breakfast

6:30-8:30
Breakfast

Session 5

Session 6

8:30-9:00 S5-1
9:00-9:30 S5-2
9:30-10:00 S5-3
Coffee break
10:15-10:45 S5-4
10:45-11:15 S5-5
11:15-11:45 S5-6

8:30-9:00 S6-1
9:00-9:30 S6-2
9:30-10:00 S6-3
Coffee break
10:15-10:45 S6-4
10:45-11:15 S6-5

Session 1
8:45-9:15 S1-1
9:15-9:45 S1-2
9:45-10:15 S1-3
Coffee break
10:30-11:00 S1-4
11:00-11:30 S1-5
11:30-12:00 S1-6

Session 3
8:45-9:15 S3-1
9:15-9:45 S3-2
9:45-10:15 S3-3
Coffee break
10:30-11:00 S3-4
11:00-11:30 S3-5
11:30-12:00 S3-6

12:00-13:30

12:00-13:30

11:45-12:15
Poster presentations

Lunch

Lunch

12:15-13:30

1

Closing session
11:15-12:00 C1

LunchSession 213:30-14:00 S2-1
14:00-14:30 S2-2
14:30-15:00 S2-3
15:00-15:30 S2-4

Session 4
13:30-14:00 S4-1
14:00-14:30 S4-2
14:30-15:00 S4-3
15:00-15:30 S4-4

15:30Free time

15:30Free time

18:00-19:30
Dinner
at “CHAME”

18:00-19:30
Dinner
at “Lemon Grass”

19:30Poster session

19:30Poster session

21:00Happy hours at

21:00Happy hours at

21:00Happy hours at

21:00Happy hours at“Sky Lounge”“Sky Lounge”“Sky Lounge”“Sky Lounge”

15:00Registration

Opening session
17:30-18:15 O1
18:15-19:00 O2
19:00-21:00
Welcome receptionPROGRAM

ORAL SESSIONS

2

13:30Excursion

19:00-21:00
BanquetJune 14thOpening session (17:30-19:00)

Session leader: Senya Matsufuji (The Jikei University School of Medicine,Japan)

17:30-18:15
O1.Polyamines support mammalian cell growth predominantly
through their requirement for the process of translation initiation
Chaim Kahana (Weizmann Institute of Science, Israel)

18:15-19:00
O2. Surveys of possible biological functions of polyaminesin Xenopus embryos, with special reference to their effects on
secondary head formation induced by microinjected beta-cateninmRNAKoichiro Shiokawa (Teikyo University, Japan)3

< Reception >June 15th

Session 1. Regulation of Cellular Polyamine Contents (8:45-12:00)

Session leader: Kazuei Igarashi (Amine Pharma Research Institute,Japan)

8:45-9:15
S1-1. Functional aspects and expression of mouse antizyme
inhibitor 2 in brain and testis
Rafael Peñafiel (University of Murcia, Spain)

9:15-9:45
S1-2. Involvement of the polyamine metabolism in actin dynamics

4and vesicle transport
Leif C. Andersson (University of Helsinki, Finland)

9:45-10:15
S1-3. Polyamine regulation of antizyme frameshifting in yeastHeather M. Wallace (University of Aberdeen, UK)<Coffee break >10:30-11:00
S1-4. Differential synthesis of 29 kDa and 24.5 kDa antizymes invascular smooth muscle cells
Itsuko Ishii (Chiba University, Japan)

5

11:00-11:30
S1-5. Analyses of antizyme 2- interacting proteins
Senya Matsufuji (The Jikei University School of Medicine, Japan)

11:30-12:00
S1-6. GSTp regulates caveolin-1 dependent polyamine uptake via
actin remodelingTakeshi Uemura (The University of Arizona, USA)

< Lunch >Session 2. Molecular Evolution and Structure (13:30-15:30)
Session leader: Akira Shirahata (Josai University, Japan)

6

13:30-14:00
S2-1. Ubiquitous polyamine-responsive translational regulation in
eukaryotes employs different mechanisms
Ivaylo P. Ivanov (University College Cork, Ireland)

14:00-14:30
S2-2. The evolution and diversification of polyamine biosynthesis
Anthony J. Michael (University of Texas Southwestern Medical School,USA)

14:30-15:00
S2-3. Crystal structure of spermidine acetyltransferase
from Escherichia coli
Hiroyoshi Matsumura (Osaka University, Japan)

15:00-15:30
S2-4. Agmatidine, an agmatine-conjugated cytidine found at theanticodon wobble position of archaeal tRNAIle essential for AUA
decodingTsutomu Suzuki (University of Tokyo, Japan)
7

< Poster Session 1 >< Dinner >June 16thSession 3. Function of Polyamine (8:45-12:00)

Session leader: Takami Oka (Wakunaga Pharmacentical Co., Ltd.,Japan)

8:45-9:15
S3-1. The spermidine/spermine N1 -acetyltransferase8overexpressing mouse ? beauty or beast as an animal model
Anne Uimari (University of Eastern Finland, Finland)

9:15-9:45
S3-2. An inducible transgenic mouse model to examine the role ofpolyamines in mammary gland development andHER2/neu-induced tumorigenesis
David J. Feith (Pennsylvania State University College of Medicine, USA)

9:45-10:15
S3-3. Spermine synthase interacts with
Glyceraldehyde-3-phosphate dehydrogenase and regulates its
activity
Yoshihiko Ikeguchi (Josai University, Japan)< Coffee break >9

10:30-11:00
S3-4. The story of hypusine and eIF5A: an essential
posttranslational modification involving a polyamine
Myung Hee Park (National Institute of Health, USA)

11:00-11:30
S3-5. Characterization of the hypusine pathway from Leishmania
donovani
Rentala Madhubala (Jawaharlal Nehru University, India)

11:30-12:00
S3-6. Promotion of longevity of mice through improvement in theintestinal environment by probiotic-induced upregulation ofpolyamines
Mitsuharu Matsumoto (Kyodo Milk Industry Co. Ltd., Japan)

10< Lunch >Session 4. Polyamines in Plants and Microorganisms (13:30-15:30)

Session leader: Lo Persson (Lund University, Sweden)

13:30-14:00
S4-1. Thermospermine enhances translation of SAC51 which is
involved in stem elongation in Arabidopsis thaliana
Taku Takahashi (Okayama University, Japan)

14:00-14:30
S4-2. High-throughput analysis of plant polyamines including
thermospermine during growth and salinity stress
Tomonobu Kusano (Tohoku University, Japan)

11

14:30-15:00
S4-3. A novel polyamine importer, YeeF, required for swarming
induced by extracellular polyamines in Escherichia coli K-12
Shin Kurihara (Kyoto Institute of Technology, Japan)

15:00-15:30
S4-4. Enigmas of biosyntheses of unusual polyamines in an
extreme thermophile, Thermus thermophilus
Tairo Oshima (Kyowa-kako Co., Japan)< Poster Session 2 >< Dinner >12June 17thSession 5. Polyamine in Medicine (8:30-11:45)

Session leader: Nakaaki Ohsawa (Aino Institute for Aging Research,Japan)

8:30-9:00
S5-1. Correlation between images of silent brain infarction, carotid
atherosclerosis and white matter hyperintensity, and plasma
levels of acrolein, IL-6 and CRP
Keiko Kashiwagi (Chiba Institute of Science, Japan)

9:00-9:30
S5-2. N1,N12-diacetylspermine in colon cancer tissues
Masao Kawakita (Tokyo Metropolitan Institute of Medical Science,Japan)

9:30-10:00
S5-3. Effects of spermine, arginine and glutamine
supplementation on lipopolysaccharide-induced bacterial
13translocation and Peyer’s patch morphology
Phan Nguyen Thanh Binh (HCMC Nutrition Center, Vietnam)<Coffee break >10:15-10:45
S5-4. Polyamination of hyphae-specific surface proteins bytransglutaminase 4 inhibits adhesion of Candida albicans toepithelial cells
In-Gyu Kim (Seoul National University College of Medicine, Korea)

10:45-11:15
S5-5. Polyamine transport in Trypanosoma cruziMarie-Pierre Hasne (Oregon Health & Science University, USA)14

11:15-11:45
S5-6. Characterization of putrescine and spermidine uptake by thehuman malaria parasite, Plasmodium falciparum
Jandeli Niemand (University of Pretoria, South Africa)

Selected Poster Presentations (11:45-12:15)

Session leaders: Ann Uimari (University of Eastern Finland, Finland) andYoshihiko Ikeguchi (Josai University, Japan)< Lunch >< Excursion >15

< Banquet Party >June 18th

Session 6. Polyamines as Target of Drug Discovery (8:30-11:15)

Session leader: Olle Heby (Umeå University, Sweden)

8:30-9:00
S6-1. ODC in pediatric neuroblastoma: Advancement ofDFMO/etoposide into a FDA-approved Phase I clinical trial
André S. Bachmann (University of Hawaii at Manoa, USA)

9:00-9:30
S6-2. Targeting polyamine metabolism to treat cancer risk factors
Eugene W. Gerner (The University of Arizona, USA)16

9:30-10:00
S6-3. Polyamine enzymatic oxidation products in cancer therapy:
Chloroquine and docetaxel potentiate their cytotoxicity
Enzo Agostinelli (SAPIENZA University of Rome, Italy)<Coffee break >10:15-10:45
S6-4. Novel absorption improving system using polyamine for oraland pulmonary administration
Masateru Miyake (Otsuka Pharmaceutical Co., Ltd., Japan)

10:45-11:15
S6-5. Small molecule inhibitors of lysine-specific demethylase 117

(LSD1) as epigenetic modulators for the treatment of cancer
Patrick M. Woster (Wayne State University, USA)

Closing session (11:15-12:00)
Session leader: Tairo Oshima (Kyowa-kako Co., Japan)

11:15-12:00
C1. Polyamine analogues targeting epigenetics in cancer therapy
Robert A. Casero, Jr. (Johns Hopkins University School of Medicine, USA)

POSTERS18

June 15th 19:30-21:00 June 16th 19:30-21:00

P1. Assay of N1-acetylpolyamine oxidase activity with
N1,N11-didansyl- norspermine as the substrate by ion-pair
reversed phase high performance liquid chromatography
Koichi Takao (Josai University, Japan)

P2. Antizyme 2 accelerates c-Myc degradation in the cells
Noriyuki Murai (The Jikei University School of Medicine, Japan)

P3. Differentiation of multipotent progenitors into common
myeloid progenitors is impaired in the liver of antizyme-1
knockout embryo
Makiko Ohkido (The Jikei University School of Medicine, Japan)

19

P4. Enhancement of translational frameshifting by hnRNP A1-likeprotein
Satoru Horiya (The Jikei University School of Medicine, Japan)

P5. Crystallization and preliminary X-ray analysis of
the Escherichia coli spermidine acetyltransferase in complex withspermidine and coenzyme A
Shigeru Sugiyama (Osaka University, Japan)

P6. Putrescine-induced conformational changes in rat
S-adenosylmethionine decarboxylase
Makiko Wada (Josai University, Japan)

P7. Docking simulation of polyamines on the HIV-1 dimerization
initiation site in the kissing-loop dimer conformation
Gota Kawai (Chiba Institute of Technology, Japan)

P8. The influence of chemical polyamines analogs, decarboxylated
ornithine and S-(adenosyl)-methionine on the polyaminesynthesis velocity in test-systems from tissues with high20

proliferation
Syatkin S.P. (Russian Peoples' Friendship University, Russia)

P9. The influence of chemical analogs of decarboxylated ornithineand S-(adenosyl)-methionine on the growth of L-cell tissue
cultureFedoronchuk T.V. (Russian Peoples' Friendship University, Russia)

P10. Effects of polyamine on early embryogenesis of Xenopus
laevisTakeshi Kondo (Teikyo University, Japan)

P11. Polyamine inhibits growth defect in hyperglycemic silkworm
Yasuhiko Matsumoto (University of Tokyo, Japan)

P12. Ribosome modulation factor, an important protein for cell
viability encoded by the polyamine modulon
Yusuke Terui (Chiba Institute of Science, Japan)

21

P13. Generation of eukaryotic translation initiation factor 5A andits post-translational modification enzyme disrupted mice
Kazuhiro Nishimura (Chiba University, Japan)

P14. Effect of intestinal absorption of macromolecules bypolyamine derivatives in rats
Yoshiaki Sugita (Josai University, Japan)

P15. Spermidine plays an important role in the regulation of
insulin synthesis and cytoplasmic Ca2+ in mouse Beta-TC6
insulin-secreting cells
Ikuko Hisanaga (Mizuno) (Wakunaga Pharmaceutical Co., Ltd, Japan)

P16. Establishment of a novel assay system for transglutaminase
using highly reactive substrate peptide and polyamine
Kiyotaka Hitomi (Nagoya University, Japan)

P17. Cadaverine covalently linked to the peptidoglycan
of Selenomonas ruminantium interacts with the major outermembrane protein Mep45 through the N-terminal SLH domain in22

the periplasmic space
Seiji Kojima (Tohoku University, Japan)

P18. Molecular biological and biochemical characterization of fivepolyamine oxidase isoforms in Arabidopsis thaliana
Yoshihiro Takahashi (Tohoku University, Japan)

P19. The dwarf phenotype of thermospermine-deficientmutant, acl5-1 is suppressed by mutations in ribosomal proteins
Jun-ichi Kakehi (Okayama University, Japan)

P20. Thermospermine and norspermine suppress xylem
differentiation in vascular plants
Hiroyasu Motose (Okayama University, Japan)

P21. Oxidation and degradation of gamma-glutamylputrescinesynthetase (PuuA) in Escherichia coli K-12
Ayaka Kambe (Kyoto Institute of Technology, Japan)

P22. Synthesis of glutathionylspermidine using
glutathionylspermidine synthetase/amidase (Gsp)23

from Escherichia coli K-12
Kanoko Hayashida (Kyoto Institute of Technology, Japan)

P23. Analysis of polyamine composition of a novel extreme
thermophile Calditerricola satsumensis YMO81Toshiyuki Moriya (Kyowa Kako Co., Ltd, Japan)

P24. Deoxyhypusine synthase-like (DSL) protein is involved
in sym-homospermidine biosynthesis in Thermus thermophilus
Yumiko Takeda (Tokyo University of Pharmacy and Life Sciences, Japan)

P25. Dual biosynthesis pathway for longer chain polyamines inhyperthermophilic archaeon Thermococcus kodakarensis
Shinsuke Fujiwara (Kwansei-Gakuin University, Japan)

P26. Identification of potential biomarkers for antimony
susceptibility / resistance in Leishmania donovani
Swati Mandal (Jawaharlal Nehru University, India)

24P27. More intensive correlation between brain infarction andacrolein threactive oxygen species
Ryotaro Saiki (Chiba University, Japan)

P28. Increased polyamine intake prolongs murine longevity
Yoh Dobashi (Jichi Medical University, Japan)

P29. Polyamine intake and Mediterranean diet
Kuniyasu Soda (Jichi Medical University, Japan)

P30. Change in the methylation status of the ITGAL promoter by
spermine possibly regulates LFA-1 expression
Yoshihiko Kano (Jichi Medical University, Japan)

P31. Effect of polyamine on factors involved in the initiation ofcancer metastasis and invasion
Shingo Tsujinaka (Jichi Medical University, Japan)

P32. No increased incidence of inflammation-associatedneoplasms after and during increased polyamine intake
Fumihiro Chiba (Jichi Medical University, Japan)

25

P33. Selection and characterization of RNA aptamers against
spermine to develop the new sensing probe of polyamine
Akihiro Oguro (The Jikei University School of Medicine, Japan)

P34. Occurrence of polyamine bodies in parallel with anticancer
drug-induced apoptosis
Masashi Shin (Sojo University, Japan)

P35. Anthracene polyamine conjugates induce cell death in humanleukaemic cells
Radiah A. Ghani (University of Aberdeen, UK)

P36. PG11047 induces mesenchymal to epithelial transition in thetrastuzumab resistant JIMT-1 cell line
Helena Cirenajwis (Lund University, Sweden)

P37. Vibrational and cytotoxic studies on a modified spermidine
and its Pd(II) complex
Tânia Silva (University of Coimbra, Portugal)

26

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