転写制御タンパク質構造解析研究チーム
Transcription Regulation Protein Crystallography Team
チームリーダーTAHIROV, Tahir H.

2004 年度，我々は引き続き Thermus thermophilus HBS および Pyrococcus horikoshii OT3 由来タンパク質の構造研究を行い，さらにこれらの機能メカニズムを解明するため，リガンドおよび補酵素との複合構造の解析も行った。現在，我々は転写因子および転写後調節に関与するタンパク質に注目している。
（12 月 1 日より研究チーム名を，構造解析第 3 研究チームから転写制御タンパク質構造解析研究チームに変更）

During the 2004–2005 ﬁscal year our team continued structural studies of proteins fromthe Thermus thermophilus HB8 and Pyrococcus horikoshii OT3. In order to understand thefunctional mechanisms of these proteins, the structures of ligand and/or cofactor bound forms
were also determined. Now we are particularly interested in proteins involved in transcription
and post transcriptional regulation of gene expression.

Research Subjects
1. Current state and future plans for high-throughput
protein crystallography

Staﬀ
Head
Dr. Tahir H. TAHIROVMembers
Dr. Peter REHSE
Mr. Eiji INAGAKIMr. Hitomi TAKAHASHI

誌

上発表

Publications［雑誌］（原著論文） ＊印は査読制度がある論文
Inagaki E., Ukita Y., Kumei M., Kajihara Y., and Tahirov
T.: “Crystallization and preliminary crystallographic
analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus”, Acta Cryst. D 60, 761–763 (2004).
＊Takahashi H., Inagaki E., Kuroishi C., and Tahir T.
H.: “Structure of the Thermus thermophilus putative periplasmic glutamate/glutamine-binding protein”,
Acta Cryst. D 60, 1846–1854 (2004). ＊Rehse P., Kuroishi C., and Tahirov T.: “Structure ofthe RNA-processing inhibitor RraA from Thermus thermophilus”, Acta Cryst. D 60, 1997–2002 (2004). ＊Perederina A., Svetlov V., Vassylyeva M. N., Tahirov T.,

1078

Yokoyama S., Artsimovitch I., and Vassylyev D. G.:“Regulation through the secondary channel: Structuralframework for ppGpp-DksA synergism during transcription”, Cell 118, 297–309 (2004). ＊Tahirov T., Inagaki E., Ohshima N., Kitao T., Kuroishi
C., Ukita Y., Takio K., Kobayashi M., Kuramitsu S.,
Yokoyama S., and Miyano M.: “Crystal structure ofpurine nucleoside phosphorylase from Thermus thermophilus”, J. Mol. Biol. 337, 1149–1160 (2004). ＊Rehse P., Ohshima N., Nodake Y., and Tahirov T.: “Crystallographic structure and biochemical analysis of theThermus Thermophilus osmotically inducible Protein
C”, J. Mol. Biol. 338, 959–968 (2004). ＊Ohshima N., Inagaki E., Yasuike K., Takio K., and Tahirov
T.: “Structure of Thermus thermophilus 2-Keto-3deoxygluconate kinase: Evidence for recognition of anopen chain substrate”, J. Mol. Biol. 340, 477–489(2004). ＊口 頭 発 表 Oral Presentations（国際会議等）
Rehse P., Ohshima N., Nodake Y., and Tahirov T.: “Crystallographic and kinetic analysis of the osmotically inducible protein C from Thermus Thermophilis HB8”,
1st Paciﬁc-Rim Int. Conf. on Protein Science (PRICPS
2004), (Protein Science Society of Japan and others),
Yokohama, Apr. (2004).
Ohshima N., Inagaki E., Kajihara Y., Yasuike K., Takio
K., and Tahir T. H.: “Structure and function of Thermus thermophilus 2-keto-3-deoxygluconate kinase”, 1st
Paciﬁc-Rim Int. Conf. on Protein Science (PRICPS
2004), (Protein Science Society of Japan and others),
Yokohama, Apr. (2004).
Tahirov T.: “Crystal structure of Pyrococcus horikoshii

平成 16 年度

NIKR: nickel sensing and implications for the regulation of promoter DNA recognition”, FASEB Summer
Research Conf. on Transcriptional Regulation During
Cell Growth, Diﬀerentiation and Development, Saxtons
River, USA, Aug. (2004).
Nishibori E., Koishi T., Narumi T., Tahirov T., AgoH., Taiji M., Miyano M., Ebisuzaki T., Makino J.,
and Sakata M.: “An x-ray structure determination bygenetic algorythm with a special-purpose computer;
MDM”, 8th Int. Conf. on Biology and Synchrotron Radiation (BSR2004), (Himeji City, RIKEN, and others),
Himeji, Sept. (2004).
Kuramitsu S., Ebihara A., Kanagawa M., Kuroishi C.,
Sato S., Agari Y., Iino H., Kashihara A., Kira S.,
Yanai H., Imagawa T., Nakagawa N., Masui R.,
Bessho Y., Hori-Takemoto C., Handa N., Kishishita S.,
Niino-kukimoto M., Kaminishi T., Wang H., MizohataE., Shibata R., Kato-Murayama M., Kawazoe M., AraiR., Toyama M., Kunishima N., Tahirov T., Sekine S.,
Shinkai A., Vassylyev D. G., Murayama K., Terada T.,
Shirouzu M., Miki K., and Yokoyama S.: “A structuraland functional whole-cell project for the model organ-

理研研究年報ism, Thermus thermophilus HB8”, 3rd Int. Conf. on
Structural Genomics (ICSG 2004), (International Structural Genomics Organization and others), WashingtonDC, USA, Nov. (2004).
Rehse P. and Tahirov T.:“Crystal structure ofa purine/pyrimidine phosphoribosyltransferase-related
protein from Thermus thermophilis HB8”, 3rd Int. Conf.
on Structural Genomics (ICSG 2004), (International
Structural Genomics Organization and others), Washington DC, USA, Nov. (2004).
Inagaki E., Ohshima N., Kuroishi C., and Tahirov
T.: “Crystal Structure of Thermus thermophilus ∆1 pyrroline-5-carboxylate dehydrogenase”, 3rd Int. Conf.
on Structural Genomics (ICSG 2004), (International
Structural Genomics Organization and others), Washington DC, USA, Nov. (2004).
（国内会議）
Bagautdinov B., 藤本弥生, Tahirov T.: “Crystal structureof indole-3-glycerol phosphate Synthase from Thermus
thermophilus HB8”, 高度好熱菌丸ごと一匹プロジェクト
第 3 回連携研究会, (理研), 播磨, 7–8 月 (2004).

1079