Catalog Number: 2610022

Murashige and Skoog Medium, powder, WithoutIAA, Kinetin, Sucrose
Physical Description: Off white powder
Chemicals used are carefully selected and, whenever possible, analytical grade materials were used.
Finished batches are checked to ensure that they conform to preset high standards before they arereleased for sale.
Except where otherwise indicated the amounts shown relate to single strength media.
Please note that in all MP's plant media, iron is added as FeNa EDTA rather than the salt specified inthe original formulation. This is to ensure that iron is available in solution over a wide pH range.
Preparation of Liquids from Powder Plant Tissue Culture Media
1. Measure out approximately 25% less purified water (distilled or deionized) than the final volume ofmedium required. The water should be at room temperature (15 oC to 30oC).
2. Stir the water and slowly add the powder. Rinse out the inside of the container to remove all tracesof powder.
Continue stirring until the powder has dissolved. Some media will not dissolve completely unless thepH is reduced. For these, lower the pH to about 3.0 to facilitate solution.
Note: It is possible to weigh out the desired quantity of powder from a container, using the weight
noted on the product label. It is recommended, however, to use an entire container at once, in whichcase it is not necessary to weigh the powder.
3. Add the required supplements and stir to disperse.
Note: Heat-labile substrates should be added at step 8, after autoclaving.
4. Check and if necessary, adjust the pH of the medium to the desired level (normally 5.5 + 0.1). Mix
gently during additions.
5. Add sufficient purified water to give a volume equal to the final volume less any heat-labile
substance to be added in step 8.
6. Add the desired quantity to agar. Heat, with continuous mixing, until the solution is clear. Do notboil. Do not allow to cool below 50oC during dispensing.
7. Dispense the medium into suitable containers, plug or cap, then autoclave at 121 oC (1 bar, 15 psi)for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are notrecommended.
8. After cooling, aseptically add desired sterile heat-labile supplements.
9. Label and store at 2oC to 8oC.

Formulation:

Component

mg/lt

Mol. Wt.

Mol.
(mM)

2.00000

75.07

0.03

myo-Inositol

100.00000

180.20.55

Niacin0.50000

123.1

0.0041

Pyridoxine HCl

0.50000

205.6

0.0024

Thiamine HCl

0.10000

337.3

0.00031650.00000

80.04

20.61

Boric Acid [H3BO3]

6.20000

61.83

0.10

Calcium Chloride [CaCl2 2H2O] (anhyd)332.0000

111

2.99

Cobalt Chloride [CoCl2 6H2O] Hexahydrate

0.02500

237.90.0001

Cupric Sulfate [CuSO4]

0.01600

159.680.0001EDTA Fe Na36.70000

367.050.10

Magnesium Sulfate [MgSO4]

180.70000

120.4

1.50

Manganese Sulfate [MnSO4 H2O] Monohydrate

16.90000

169.013

0.11

Potassium Iodide [KI]0.83000

1660.01

Potassium Nitrate [KNO3]

1900.00000

101.1

18.79

Potassium Phosphate Monobasic [KH2PO4]

170.00000

136.09

1.25

Sodium Molybdate [Na2MoO4 2H2O] Dihydrate

0.25000

241.9

0.0010Zinc Sulfate [ZnSO4 7H2O] Heptahydrate

8.60000

287.5

0.03

Amino Acids
GlycineVitamins

Inorganic Salts
Ammonium Nitrate [NH4NO3]

CEL/1710