REF: P30902

CELL LINES- ACE2 HEK293 CELL LINE -Product Name:

ACE2 HEK293 cell line

Catalog Number:

P30902Cell Line:

HEK293

Resistance:

Hygromycin

Format:>3x106 cells in Cryopreserved vials

Storage:

Liquid Nitrogen

ACE2 receptor and COVID-19

ACE2 HEK293 cell line
The ACE2 HEK293 cell line has been

ACE2 is the main host cell receptor of novel

developed by stable transfection with acoronavirus from human airway epithelial

human Angiotensin-converting enzyme 2cells named 2019-nCoV. ACE2 is the key(hACE2)

receptor

expression

plasmid.

receptor in the entry of virus into the cell to

hACE2

HEK293cellprovides

cause the final infection.

line

consistent levels of expression of humanTheACE2 protein in cells surface.

glycoprotein on the envelope to bind to theircellularcoronaviruses
ACE2

use

receptors

thethrough

spike
their

receptor binding domain (RBD).
The study of the interaction between thecoronavirus and the ACE2 receptor plays acrucial role in the findings of possible
therapeutic strategies to treat the COVID19 disease.
Bibliography: Xu, H., Zhong, L., Deng, J. et al. Highexpression of ACE2 receptor of 2019-nCoV on the epithelial
cells of oral mucosa. Int J Oral Sci 12, 8 (2020).
https://doi.org/10.1038/s41368-020-0074-x

This cell line is intended to be used as an

V.A.T. Nº:ESB95481909“in vitro” model for research studies.

INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS, S.L.
Parque Tecnológico Bizkaia, Edf. 502, 1ª Planta | 48160 | Derio | Bizkaia
Tel.: +34 944005355 | Fax: +34 946013455
innoprot@innoprot.com | www.innoprot.com

Lan, J., Ge, J., Yu, J. et al. Structure of the SARS-CoV-2 spikereceptor-binding domain bound to the ACE2 receptor. Nature(2020). https://doi.org/10.1038/s41586-020-2180-5

Immunofluorescence analysis

RT-PCR analysis

The detection of ACE2 protein in the cells

Thesurface

analyzed by RT-PCR.

wascarried

outby

presence

ofACE2mRNA

wasimmunofluorescence analysis with an ACE2
primary antibody and a FITC secondary
antibody.

Figure 1. ACE2 RT-PCR anaylisis. (1) ACE2 HEK293
cell line. Positive Control (C+): ACE2 cDNA. NegativeControl (C-): not transfected HEK293 cells.

Quality Control
All cells are performance assayed and test
negative for mycoplasma, bacteria, yeastand fungi. Cell viability, morphology andproliferative capacity are measured after
recovery from cryopreservation. Innoprot
guarantees stable expression for many
generations and provides support for cell
Figure 2. Immunofluorescence assay. The

culture and visualization.

image in the upper panel shows the membrane
localization of ACE2 in HEK293 cell line. The

THIS PRODUCT IS FOR RESEARCH PURPOSES ONLY. It

image in the lower panel shows bright field.

is not to be used for drug or diagnostic purposes, nor is it

カタログ番号BBP30902

品

名ACE2 HEK293

容量3x10^6cells/vial

保存

液体窒素

intended for human use. Innoprot products may not be resold,
modified for resale, or used to manufacture commercial
products without written approval of Innovative Technologies
in Biological Systems, S.L.

価格（円・税抜） 820,000
融解用培地：D-MEM(High Glucose)＋10%FBS
培養用培地：D-MEM(High Glucose)＋10%FBS＋1%NEAA＋Hygromycin(80ug/mL)
V.A.T. Nº:
ESB95481909

操作法につきましては、別資料にてご確認ください。

INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS, S.L.
Parque Tecnológico Bizkaia, Edf. 502, 1ª Planta | 48160 | Derio | Bizkaia

本細胞の提供には、MTAが必要になります。
Tel.: +34 944005355 | Fax: +34 946013455
innoprot@innoprot.com | www.innoprot.com

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