Publications
http://www.researcherid.com/rid/E-5989-2012

1.

Structure Analysis of a New Type of Vanadium Oxide by High Resolution Electron Microscopy.
Y. Fujiyoshi, K. Ishizuka and N. Uyeda
J. Electron microsc., 26, 47-49 (1977).

2.

The Alumina Supermicrogrid for High Resolution Electron Microscopy.
Y. Fujiyoshi, and N. Uyeda
J. Electron microsc., 27, 75-82 (1978).

3.

High Voltage Electron Microscopy for Image Discrimination of Constituent Atoms in Crystals andMolecules.
N. Uyeda, T. Kobayashi, K. Ishizuka and Y. Fujiyoshi
Chemica Scripta., 14, 47-61 (1978/79).

4.

Crystal Structure of Ag-TCNQ.
N. Uyeda, T. Kobayashi, K. Ishizuka and Y. Fujiyoshi
Nature, 285, 95-97 (1980).

5.

A New Method for Optimal-Resolution Electron Microscopy of Radiation-Sensitive Specimens.
Y. Fujiyoshi, T. Kobayashi, K. Ishizuka, N. Uyeda, Y. Ishida and Y. Harada
Ultramicroscopy, 5, 459-468 (1980).

6.

Digital Reconstruction of Bright Field Phase Contrast Images from High Resolution Electron
Micrographs.
E. J. Kirkland, B. M. Siegel, N. Uyeda and Y. Fujiyoshi
Ultramicroscopy, 5, 479-503 (1980).

7.

Direct Imaging of Double-Strand DNA Molecule.
Y. Fujiyoshi and N. Uyeda
Ultramicroscopy, 7, 189-192 (1981).

8.

Fixation of Skeletal Muscle Actin in f-State by Chemical Cross-Linking with Bis-Imidoesters.
O. Ohara, S. Takahashi, T. Ooi and Y. Fujiyoshi
Biochem. Biophys. Research Communications, 100, 988-994 (1981).

9.

High Resolution TEM Images of Zinc-Phthalocyanine Polymorphs in Thin Films.
T. Kobayashi, Y. Fujiyoshi, K. Ishizuka and N. Uyeda
Acta Cryst. A37, 692-697 (1981).

10.

Studies of Poly--Methyl-L-Glutamate Monolayers by Infrared ATR and Transmission Spectroscopy
and Electron Microscopy.
F. Takeda, M. Matsumoto, T. Takenaka and Y. Fujiyoshi
J. Colloid and Interface Science, 84, 220-227 (1981).

11.

Visualization of the DNA Thread Packing within Bacteriophage T4 Heads.
Y. Fujiyoshi, H. Yamagishi, T. Kunisada, H. Sugisaki, T. Kobayashi and N. Uyeda
J. Ultrastructure Research, 79, 235-240 (1982).

12.

Cross-Linking Study on Skeletal Muscle Actin: Properties of Suberimidate-Treated Actin.
O. Ohara, S. Takahashi, T. Ooi and Y. Fujiyoshi
J. Biochem., 91, 1999-2012 (1982).

13.

Surface Enhanced Raman Scattering of Citrate Ions Adsorbed on Gold Sol Particles.
M. Mabuchi, T. Takenaka, Y. Fujiyoshi and N. Uyeda
Surface Science, 119, 150-158 (1982).

14.

The Observation of Molecular Orientation in Crystal Defects and Growth Mechanism of Thin
Phthalocyanine Films.
T. Kobayashi, Y. Fujiyoshi and N. Uyeda
Acta Cryst., A38, 356-362 (1982).

15.

Nonlinear High Resolution Image Processing of Conventional Transmission Electron Micrograph, II
Experiment.
E. J. Kirkland, B. M. Siegel, N. Uyeda and Y. Fujiyoshi
Ultramicroscopy, 9, 65-74 (1982).

16.

Surface Pressure Dependence on Monolayer Structure of Poly--Benzyloxycarbonyl-L-Lysine.
F. Takeda, M. Matsumoto, T. Takenaka, Y. Fujiyoshi and N. Uyeda
J. Colloid and Interface Science, 91, 267-271 (1983).

17.

High-Resolution Electron Microscopy of Structural Defects in Organic Crystals.
T. Kobayashi, Y. Fujiyoshi and N. Uyeda
J. Crystal Growth, 65, 511-517 (1983).

18.

High Resolution Observation of Cellulose Microfibrils.
J. Sugiyama, H. Harada, Y. Fujiyoshi and N. Uyeda
Mokuzai Gakkaishi, 30, 98-99 (1984).

19.

Electron Microscopy of tRNA Crystals I: Thin Crystals Negatively Stained with Uranyl Acetate.
Y. Fujiyoshi, K. Morikawa, N. Uyeda, H. Ozeki and H. Yamagishi
Ultramicroscopy, 12, 201-212 (1984).

20.

Electron Microscopy of tRNA Crystals II:
4Å Resolution Diffraction Pattern and Substantial Stability to Radiation Damage.
Y. Fujiyoshi, N. Uyeda, K. Morikawa and H. Yamagishi
J. Mol. Biol., 172, 347-354 (1984).

21.

Surface Enhanced Raman Scattering by Isomeric Monobromopyridines Adsorbed on Gold and Silver
Sol Particles.
T. Takenaka, E. Eda, M. Mabuchi, Y. Fujiyoshi and N. Uyeda
Bull. Inst. Chem. Res., Kyoto Univ., 62, 219-232 (1984).

22.

High Resolution Imaging and Interpretation of Regular and Irregular Structures in n-Niobium
Pentoxide Crystal.
N. Uyeda, Y. Fujiyoshi and K. Ishizuka
Ultramicroscopy, 15, 139-150 (1984).

23.

Various Types of 5S rRNA Crystals as Studied by X-ray Diffraction and Electron Microscopy.
K. Morikawa, Y. Fujiyoshi, K. Ishizuka, M. Kwakami and S. Takemura
Nucleic Acids Res., 15, 143-146 (1984).

24.

Improved High Resolution Image Processing of Bright Field Electron Micrographs: Part II Experiment.
E. J. Kirkland, B. M. Siegel, N. Uyeda and Y. Fujiyoshi
Ultramicroscopy, 17, 87-104 (1985).

25.

Some Remarks on Imogolite Mesophase.
K. Kajiwara, N. Donkay, Y. Fujiyoshi and H. Inagaki
Bull. Inst. Chem. Res., Kyoto Univ., 63, 320-331 (1985).

26.

Observations of Cellulose Microfibrils in Valonia macrophysa by High resolution Electron
Microscopy.
J. Sugiyama, H. Harada, Y. Fujiyoshi and N. Uyeda
Mokuzai Gakkaishi, 31, 61-67 (1985).

27.

Direct Molecular Imaging of Low Dimensional Solids by High Resolution Electron Microscopy.
N. Uyeda, T. Kobayashi, K. Ishizuka, Y. Fujiyoshi, H.Inockuchi and G. Saito
Mol. Cryst. Liq. Cryst., 125, 103-112 (1985).

28.

Electron Microscopic Study of 5S rRNA Crystals from Thermus thermophilus HB8.
K. Morikawa, Y. Fujiyoshi, K. Ishizuka, M. Kawakami and S. Takemura
Journal of Microscopy, 142, 247-258 (1985).

29.

Lattice Imagies from Ultrathin Sections of Cellulose Microfibrils in the Cell Wall of Valonia
Macrophysa Kütz.
J. Sugiyama, H. Harada, Y. Fujiyoshi and N. Uyeda
Planta, 166, 161-168 (1985).

30.

Lyotropic Mesophase of Imogolite, 2 Microscopic Observation of Imogolite Mesophase.
K. Kajiwara, N. Donkai, Y. Fujiyoshi and H. Inagaki
Makromol. Chem. 187, 2895-2907 (1986).

31.

Holes in a Stearic Acid Monolayer Observed by Dark-Field Electron Microscopy.
N. Uyeda, T. Takenaka, K. Aoyama, M. Matsumoto and Y. Fujiyoshi
Nature, 327, 319-321 (1987).

32.

Formation and Structure of a 2,3,9,10,16,17,23,24-Octacyanophalocyanine-Potassium Complex inThin Film.
M. Ashida, Y. Uyeda, H. Yanagi, N. Uyeda, Y. Fujiyoshi and J. R. Fryer
Acta Crystallographica, B44, 146-151 (1988).

33.

Direct Observation of Gold Sol by Cryo-Electron Microscopy.
Y. Tahara and Y. Fujiyoshi
Bull. Inst. Chem. Res., Kyoto Univ., 66, 598-604 (1989).

34.

High Resolution Cryo-Electron Microscopy for Biological Macromolecules.
Y. Fujiyoshi
J. Electron Microsc., 38, 97-101 (1989).

35.

Image Deconvolution of a Single High-Resolution Electron Micrograph.
Y. Liu, S. Xiang, H. Fan, D. Tang, F. Li, Q. Pan, N. Uyeda and Y. Fujiyoshi
Acta Crystallographica, A46, 459-463 (1990).

36.

Image Resolution Enhancement by Combining Information from Electron Diffraction Pattern andMicrograph.
H. Fan, S. Xiang, F. Li, Q. Pan, N. Uyeda and Y. Fujiyoshi
Ultramicroscopy, 36, 361-365 (1991).

37.

Development of a Superfluid Helium Stage for High-Resolution Electron Microscopy.
Y. Fujiyoshi, T. Mizusaki, K. Morikawa, H. Yamagishi, Y. Aoki, H. Kihara and Y. Harada
Ultramicroscopy, 38, 241-251 (1991).

38.

A Method for Observing Cross-Sectional Views of Biomembranes.
K. Sakata, Y. Tahara, K. Morikawa, Y. Fujiyoshi and Y. Kimura
Ultramicroscopy, 45, 253-261 (1992).

39.

Functional Signal Peptide Reduces Bilayer Thickness of Phosphatidylcholine Liposome.
Y. Tahara, M. Murata, S. Onishi, Y. Fujiyoshi, M. Kikuchi, and Y. Yamamoto
Biochemistry, 31, 8747-8754 (1992).

40.

Methods for Orienting Purple Membrane in Vitreous Ice.
K. Sakata, Y. Kimura and Y. Fujiyoshi
Bull. Inst. Chem. Res., 70, 257-269 (1992).

41.

Dark-Field Electron Microscopy of Langmuir-Blodgett Films of Fatty Acids and Their Barium Salts.
M. Matsumoto, N. Uyeda, Y. Fujiyoshi and K. Aoyama
Thin Solid Films, 223, 358-367 (1993).

42.

A pH Induced Two-Dimensional Crystal of Membrane-Bound Na , K -ATPase of Dog Kidney.
Y. Tahara, S Ohnishi, Y. Fujiyoshi, Y. Kimura and Y. Hayashi
FEBS Letters, 320, 17-22 (1993).

43.

Epitaxial Growth of Tetraphenylmetalporphyrin Films Vapor-Deposited on Alkali Halides.
S. Hyashi, H. Yanagi, Y. Ueda, Y. Fujiyoshi and M. Ashida
Ultramicroscopy, 49, 308-317 (1993).

44.

Fine Structure of Influenza A Virus Observed by Electron cryo-Microscopy.
Y. Fujiyoshi, N. P. Kume, K. Sakata and S. B. Sato
EMBO Journal, 13, 318-326 (1994).

45.

Projection Structure of Human Immunodeficiency Virus Type 1 (HIV-1) Observed with High
Resolution Electron Cryo-Microscopy.
T. Goto, T. Ashina, Y. Fujiyoshi, N. P. Kume, H. Yamagishi and M. Nakai
J. Electron Microsc., 43, 16-19 (1994).

46.

Atomic Model of Plant Light-Harvesting Complex by electron crystallography.
W. Kühlbrandt, Da Neng Wang and Y. Fujiyoshi
Nature, 367, 614-621 (1994).

+

+

47.

A new method to measure bilayer thickness: cryo-electron microscopy of frozen hydrated liposomes
and image simulation.
Y. Tahara and Y. Fujiyoshi
Micron, 25, 141-149 (1994).

48.

Characterization of a de novo Designed Protein.
T. Tanaka, H. Kimura, M. Hayashi, Y. Fujiyoshi, K. Fukuhara and H. Nakamura
Protein Science, 3, 419-427 (1994).

49.

A Method for 2D-Crystallization of Soluble Proteins at Liquid-Liquid Interface.
K. Aoyama, K. Ogawa, Y. Kimura and Y. Fujiyoshi
Ultramicroscopy, 57, 345-354 (1995).

50.

Cryogenic Transmission Electron Microscopic Studies of Micellar Structure Correlated with Solution
Viscosity on Perfluorooctyl Sulfonates and Their Mixtures with a Nonionic Surfactant.
A. Knoblich, M. Matsumoto, K. Murata and Y. Fujiyoshi
Langmuir, 11, 2361-2366(1995).

51.

The Structure of the R-type Straight Flagellar Filament of Salmonella at 9Å Resolution by Electron
Cryomicroscopy.
Y. Mimori, I. Yamashita, T. Akiba, K. Murata, Y. Fujiyoshi, K. Yonekura,
C. Toyoshima and K. Namba
J. Mol. Biol., 249, 69-87 (1995).

52.

Observation of Micelles of Surfactin, an Anionic Lipopeptide, with Electron Cryo-Microscope.
A. Knoblich, M. Matsumoto, R. Ishiguro, K. Murata, Y. Fujiyoshi, Y. Ishigami and M. Osman
Colloids and Surfaces B; Biointerfaces, 5, 43-48 (1995).

53.

Two-Dimensional Crystals of the Kdp-ATPase of Escherichia coli.
A. H. Iwane, I.Iikeda,Y. Kimura, Y. Fujiyoshi, K. Altendorf and W. Epstein
FEBS Letters, 396, 172-176 (1996).

54.

Direct Interaction of Flagellin Termini Essential for Polymorphic Ability of Flagellar Filament.
Y. M. Kiyosue, F.Vonderviszt, I.Yamashita, Y. Fujiyoshi, and K.Namba
Proc. Natl. Acad. Sci. USA, 93, 15108-15113 (1996).

55.

Examination of the LeafScan 45, a Line-Illuminating Micro-Densitometer, for Its Use in Electron
Crystallography.
K. Mitsuoka, K. Murata, Y. Kimura, K. Namba, Y. Fujiyoshi
Ultramicroscopy, 68, 109-121 (1997).

56.

The Three-Dimensional Structure of Aquaporin 1.
T. Walz, T. Hirai, K. Murata, J. B. Heymann, K. Mitsuoka, Y. Fujiyoshi, B. L. Smith,
P. Agre and A. Engel
Nature, 387, 624-627 (1997).

57.

Surface of Bacteriorhodopsin Revealed by High-Resolution Electron Crystallography.
Y. Kimura, D. G. Vassylyev, A. Miyazawa, A. Kidera, M. Matsushima, K. Mitsuoka,
K. Murata, T. Hirai and Y. Fujiyoshi

Nature, 389, 206-211 (1997).
58.

The 3.0 Å Projection Structure of Microsomal Glutathione Transferase as Determined by Electron
Crystallography of p 21212 Two-dimensional Crystals.
H. Hebert, I. Schmidt-Krey, R. Morgenstern, K. Murata, T. Hirai, K. Mitsuoka and Y. Fujiyoshi
J. Mol. Biol., 271, 751-758 (1997).

59.

High Resolution Structure of Bacteriorhodopsin Determined by Electron Crystallography.
Y. Kimura, D. G. Vassylyev, A. Miyazawa, A. Kidera, M. Matsushima, K. Mitsuoka,
K. Murata, T. Hirai and Y. Fujiyoshi
Photochemistry and Photobiology, 66, 764-767 (1997).

60.

Characterization of Human Endothelin B Receptor and Mutant Receptors Expressed in Insect Cells.
T. Doi, Y. Hiroaki, I. Arimoto, Y. Fujiyoshi, T. Okamoto, M. Satoh and Y. Furuichi
Eur. J. Biochem, 248, 139-148 (1997).

61.

Details of the Arrangement of the Outer Capsid of Rice Dwarf Phytoreovirus, as Visualized by Twodimensional Crystallography.
Y. Zhu, A. M. Hemmings, K. Iwasaki, Y. Fujiyoshi, B. Zhong, J. Yan, M. Isogai
and T. Omura
J. Virol. 71, 8899-8901 (1997).

62.

Projection Map of the Reaction Center-Light Harvesting Complex from Rhodopseudomanas Viridis
at 10 Å resolution.
I. Ikeda, T. Odahara, K. Mitsuoka, Y. Fujiyoshi and K. Murata
FEBS Letters, 425, 505-508 (1998).

63.

Expression, Purification, and Reconstitution of Receptor for Pituitary Adenylate Cyclase-activating
Polypeptide = Large-scale Purification of a Functionally Active G protein-coupled Receptor Producedin Sf9 Insect Cells =.
T. Ohtaki, K. Ogi, Y. Masuda, K. Mitsuoka, Y. Fujiyoshi, C. Kitada, H. Sawada,
H. Onda and M. Fujino
J. Biol. Chem., 273, 15464-15473 (1998).

64.

Role of the Outermost Subdomain of Salmonella Flagelline in the Filament Structure Revealed byElectron Cryomicroscopy.
Y. M. Kiyosue, I. Yamashita, Y. Fujiyoshi, S. Ymaguchi and K. Namba
J. Mol. Biol., 284, 521-530 (1998).

65.

Interactions of Endothelin Receptor Subtypes A and B with Gi, Go, and Gq in Reconstituted
Phospholipid Vesicles.
T. Doi, H. Sugimoto, I. Arimoto, Y. Hiroaki and Y. Fujiyoshi
Biochemistry, 38, 3090-3099 (1999).

66.

Influence of Various Nucleotides on the in situ Crystallization of Ca2+-ATPase.
Y. Hiroaki, K. Mitsuoka, Y. Cheng, H. Hiroaki and Y. Fujiyoshi
Biochemica et Biophysica Acta, 1415, 361-368 (1999).

67.

The Structure of Bacteriorhodopsin at 3.0 Å Resolution Based on Electron Crystallography:
Implication of the Charge Distribution.

K. Mitsuoka, T. Hirai, K. Murata, A. Miyazawa, A. Kidera, Y. Kimura and Y. Fujiyoshi
J. Mol. Biol., 286, 861-882 (1999).
68.

Nicotinic Acetylcholine Receptor at 4.6 Å Resolution: Transverse Tunnels in the Channel Wall.
A. Miyazawa, Y. Fujiyoshi, M. Stowell and N. Unwin
J. Mol. Biol., 288, 765-786 (1999).

69.

Trehalose Embedding Technique for High-resolution Electron Crystallography.
T. Hirai, K. Murata, K. Mitsuoka, Y. Kimura and Y. Fujiyoshi
J. Electron Microsc., 48, 653-658 (1999).

70.

Molecular Structure of Proton Pump Revealed with Electron Crystallography.
Y. Fujiyoshi
The FASEB Journal., 13, s191-s194 (1999).

71.

The Structure of Aquaporin-1 at 4.5 Å Resolution Reveals Short -Helices in the Center of theMonomer.
K. Mitsuoka, K. Murata, T Walz, T. Hirai, P. Agre, J. B. Heymann, A. Engeland Y. Fujiyoshi
J. Structural Biol., 128, 34-43 (1999).

72.

W276 Mutation in the Endothelin Receptor subtype B Impairs Gq Coupling but not Gi or Go
Coupling.
F. Imamura, I. Arimoto, Y. Fujiyoshi and T. Doi
Biochemistry, 39, 686-692 (2000).

73.

The 11 Å resolution projection map of Na+/K+-ATPase calculated by application of single particle
analysis to two-dimensional crystal images.
Y. Tahara, A. Oshima, T. Hirai, K. Mitsuoka, Y. Fujiyoshi and Y. Hayashi
J. Electron Microsc., 49, 583-587 (2000).

74.

The fold of human aquaporin 1.
B. L. de Groot, J. B. Heymann, A. Engel, K. Mitsuoka, Y. Fujiyoshi and H. Grubmueller
J. Mol. Biol. 300, 987-994 (2000).

75.

Structural determinants of water permeation through aquaporin-1.
K. Murata, K. Mitsuoka, T. Hirai, T. Walz, P. Agre, J. B. Heymann, A. Engeland Y. Fujiyoshi
Nature, 407, 599-605 (2000).

76.

The three-dimensional map of microsomal glutathione transferase 1 at 6 Å resolution.
I. Schmidt-Krey, K. Mitsuoka, T. Hirai, K. Murata, Y. Cheng, Y. Fujiyoshi,
R. Morgenstern and H. Hebert
EMBO Journal, 19, 6311-6316 (2000).

77.

The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities.
C. Sato, Y. Ueno, K. Asai, K. Takahashi, M. Sato, A. Engel and Y. Fujiyoshi
Nature, 409, 1047-1051 (2001).

78.

Ligand Binding of the Second PDZ Domain Regulates Clustering of PSD-95 with the Kvl.4

Potassium Channel.
F. Imamura, S. Maeda, T. Doi and Y. Fujiyoshi
J. Biol. Chem., 277, 3640-3646 (2002).
79.

Crystal structure of Homer 1 family conserved region reveals the interaction between EVH1
domain and own praline-rich motif.
K. Irie, T. Nakatsu, K. Mitsuoka, A. Miyazawa, K. Sobue, Y. Hiroaki, T. Doi,Y. Fujiyoshi and H. Kato
J. Mol. Biol., 318, 1117-1126 (2002).

80.

Functional role of internal water molecules in rhodopsin revealed by x-ray crystallography.
T. Okada, Y. Fujiyoshi, M. Silow, J. Navarro, E.M. Laudau and Y. Shichida
Proc. Natl. Acad. Sci. USA, 99, 5982-5987 (2002).

81.

Activation of the Nicotinic acetylcholine receptor involves a switch in conformation of the subunits.
N. Unwin, A. Miyazawa, J.Li and Y. Fujiyoshi
J. Mol. Biol., 319, 1165-1176 (2002).

82.

A new technique to co-localise membrane proteins with Homer/vesl.
Y. Hiroaki, K. Nishikawa, K. Mitsuoka, T. Tachibana, K. Sobue, T. Doi and Y. Fujiyoshi
Biochem. Biophys. Research Communications, 295, 756-765 (2002).

83.

Roles of Met-34, Cys-64, and Arg-75 in the Assembly of Human Connexin 26.
A. Oshima, T. Doi, K. Mitsuoka, S. Maeda and Y. Fujiyoshi
J. Biol. Chem., 278, 1807-1816 (2003).

84.

Regulated Interaction of Endothelin B Receptor with Caveolin-1.
T. Yamaguchi, Y. Murata, Y. Fujiyoshi and T. Doi
Eur. J. Biochem 270, 1816-1827 (2003).

85.

Structure and gating mechanism of the acetylcholine receptor pore.
A. Miyazawa, Y. Fujiyoshi and N. Unwin
Nature, 423, 949-955 (2003).

86.

Inositol 1,4,5-trisphosphate receptor contains multiple cavities and L-shaped ligand-binding domains.
C. Sato, K. Hamada, T. Ogura, A. Miyazawa, K. Iwasaki, Y. Hiroaki, K. Tani,
A. Terauchi, Y. Fujiyoshi and K. Mikoshiba
J. Mol. Biol. 336, 155-164 (2004).

87.

Improved Specimen Preparation for Cryo-Electron Microscopy using a Symmetric Carbon Sandwich
Technique.
N. Gyobu, K. Tani, Y. Hiroaki, A. Kamegawa, K. Mitsuoka, and Y. Fujiyoshi
J. Structural Biol., 146, 325-333(2004).

88.

Characterization and application of monoclonal antibodies against human endothelin B receptor
expressed in insect cells.
T. Yamaguchi, I. A. Tahara, Y. Fujiyoshi and T. Doi
Biotechnology Letters, 26, 293-299 (2004).

89.

Proteomic analysis revealed a novel synaptic proline-rich membrane protein (PRR7) associated with
PSD-95 and NMDA receptor.
Y. Murata, T. Doi, H. Taniguchi, and Y. Fujiyoshi
Biochem. Biophys. Research Communications, 327, 183-191 (2005).

90.

Expression and localization of an exogenous G protein-coupled receptor fused with the rhodopsin
C-terminal sequence in the retinal rod cells of knockin mice.
T. Kodama, H. Imai, T. Doi, O. Chisaka, Y. Shichida and Y. Fujiyoshi
Experim. Eye Res., 80, 859-869 (2005).

91.

Electron tomography reveals diverse conformations of integrin αIIbβ3 in the active state.
K. Iwasaki, K. Mitsuoka, Y. Fujiyoshi, Y. Fujisawa, M. Kikuchi, K. Sekiguchi andT. YamadaJ. Structural. Biol., 150, 259-267 (2005).

92.

Lipid-protein interactions in double-layered two-dimensional AQP0 crystals.
T. Gonen, Y. Cheng, P. Sliz, Y. Hiroaki, Y. Fujiyoshi, S. C. Harrison and T. WalzNature, 438, 633-638 (2005).

93.

Essential Contribution of the Ligand-Binding βB/βC Loop of PDZ1 and PDZ2 in the Regulationof Postsynaptic Clustering, Scaffolding, and Localization of Postsynaptic Density-95.
M. Nonaka, T. Doi, Y. Fujiyoshi, S. Takemoto-Kimura and H. Bito
J. Neurosci., 26, 763-774 (2006).

94.

Two-dimensional crystallization and analysis of projection images of intact Thermus thermophilus
V-ATPase.
C. Gerle, K. Tani, K. Yokoyama, M. Tamakoshi, M. Yoshida, Y. Fujiyoshi and K. Mitsuoka
J. Struct. Biol., 153, 200-206 (2006).

95.

Implications of the Aquaporin-4 Structure on Array Formation and Cell Adhesion.
Y. Hiroaki, K. Tani, A. Kamegawa, N. Gyobu, K. Nishikawa, H. Suzuki, T. Walz,
S. Sasaki, K. Mitsuoka, K. Kimura, A. Mizoguchi and Y. Fujiyoshi
J. Mol. Biol., 355, 628-639 (2006).

96.

Structural Basis for Detoxification and Oxidative Stress Protection in Membranes.
P. J. Holm, P. Bhakat, C. Jegerschold, N. Gyobu, K. Mitsuoka, Y. Fujiyoshi, R. Morgensternand H. Hebert
J. Mol. Biol., 360, 934-945 (2006).

97.

Neurosteroid pregnenolone sulfate enhances glutamatergic synaptic transmission by facilitatingpresynaptic calcium currents at the calyx of Held of immature rats.
T. Hige, Y. Fujiyoshi and T. Takahashi
Eur. J. Neurosci., 24, 1955-1966 (2006).

98.

Aquaporin-11 containing a divergent NPA motif has normal water channel activity.
K. Yakata, Y. Hiroaki, K. Ishibashi, E. Sohara, S. Sasaki, K. Mitsuoka and Y. Fujiyoshi
Biochim. Biophys. Acta., 1768, 688-693 (2007)

99.

The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas.
K. Mio, T. Ogura, S. Kiyonaka, Y. Hiroaki, Y. Tanimura, Y. Fujiyoshi, Y. Mori and C. Sato
J. Mol. Biol., 367, 373-383 (2007)

100. Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule.
A. Oshima, K. Tani, Y. Hiroaki, Y. Fujiyoshi and G. E. Sosinsky
PNAS, 104, 10034-10039 (2007)
101.

Simulation of charge effects on density maps obtained by high-resolution electron crystallography.
T. Hirai, K. Mitsuoka, A. Kidera and Y. Fujiyoshi
J. Electron Microsc., 56, 131-140 (2007).

102.

Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus
thermophilus.
M. Toei, C. Gerle, M. Nakano, K. Tani, N. Gyobu, M. Tamakoshi, N. Sone, M. Yoshida, Y.
Fujiyoshi, K. Mitsuoka and K. Yokoyama
PNAS, 104, 20256-20261 (2007)103.

Formation of aquaporin-4 arrays is inhibited by palmitoylation of N-terminal cysteine residues.
H. Suzuki, K. Nishikawa, Y. Hiroaki and Y. Fujiyoshi
Biochem. Biophys. Acta., 1778, 1181-1189 (2008).

104.

Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle.
T. Nishizawa, K. Abe, K. Tani and Y. Fujiyoshi
J. Structural. Biol., 162, 219-228 (2008).

105.

Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent
assay.
S. Hayakawa, M. Mori, A. Okuta, A. Kamegawa, Y. Fujiyoshi, Y. Yoshiyama, K. Mitsuoka,
K. Ishibashi, S. Sasaki, T. Hattori and S. Kuwabara
J. Neuroimmun., 196, 181-187 (2008).

106.

Sendai virus F glycoprotein induces IL-6 production in dendritic cells in a fusion-independent manner.
H. Suzuki, M. Kurooka, Y. Hiroaki, Y. Fujiyoshi and Y. Kaneda
FEBS Lett., 582, 1325-1329 (2008).

107.

Projection Structure of a N-terminal Deletion Mutant of Connexin 26 Channel with Decreased Central
Pore Density.
A. Oshima, K. Tani, Y. Hiroaki, Y. Fujiyoshi and G. E. Sosinsky
Cell Commun. Adhes., 15, 85-93 (2008).

108.

Dual inhibition of SNARE complex formation by tomosyn ensures controlled neurotransmitter release.
T. Sakisaka, Y. Yamamoto, S. Mochida, M. Nakamura, K. Nishikawa, H. Ishizaki, M.
Okamoto-Tanaka, J. Miyoshi, Y. Fujiyoshi, T. Manabe and Y. Takai
J. Cell Biol., 183, 323-337 (2008).

109.

Pleomorphic configuration of the trimeric capsid proteins of Rice dwarf virus that allows formation ofboth the outer capsid and tubular crystals.
K. Iwasaki, N. Miyazaki, L. Hammar, Y. Zhu, T. Omura, B. Wu, F. Sjöborg, K. Yonekura, K.
Murata, K. Namba, D. L. Caspar, Y. Fujiyoshi and R. H. Cheng.
J. Mol. Biol., 383, 252-265 (2008).

110.

Reconstruction of the P2X2 Receptor Reveals a Vase-Shaped Structure with Lateral Tunnels above
the Membrane.

K. Mio, T. Ogura, T. Yamamoto, Y. Hiroaki, Y. Fujiyoshi, Y. Kubo and C. Sato
Structure, 17, 266-275 (2009).
111.

Acetazolamide reversibly inhibits water conduction by aquaporin-4.
Y. Tanimura, Y. Hiroaki and Y. Fujiyoshi
J. Struct. Biol., 166, 16-21 (2009).

112.

Structure of the connexin-26 gap junction channel at 3.5 Å resolution.
S. Maeda, S. Nakagawa, M. Suga, E. Yamashita, A. Oshima, Y. Fujiyoshi and T. Tsukihara
Nature, 458, 597-602 (2009).

113.

Mechanism of Aquaporin-4's Fast and Highly Selective Water Conduction and Proton Exclusion.
K. Tani, T. Mitsuma, Y. Hiroaki, A. Kamegawa, K. Nishikawa, Y. Tanimura and Y. Fujiyoshi
J. Mol. Biol., 389, 694-706 (2009).

114.

Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.
K. Abe, K. Tani, T. Nishizawa and Y. Fujiyoshi.
EMBO J., 28, 1637-1643 (2009).

115.

Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii.
Y. Kuwahara, S. Unzai, T. Nagata, Y. Hiroaki, H. Yokoyama, I. Matsui, T. Ikegami, Y. Fujiyoshi
and H.Hiroaki
Biophys. J., 97, 2034-2043 (2009).

116.

Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2
Potassium Channel.
K. Noma, K. Kimura, K. Minatohara, H. Nakashima, Y. Nagao, A. Mizoguchi and Y. Fujiyoshi
J. Biol. Chem, 284., 33139-33150 (2009).

117.

Calyculin A-induced neurite retraction is critically dependent on actomyosin activation but not on
polymerization state of microtubules.
A. Inutsuka, M. Goda and Y. Fujiyoshi
Biochem. Biophys. Res. Commun.,390, 1160-1166 (2009).

118.

Comparative study of the gating motif and C-type inactivation in prokaryotic voltage-gated sodium
channels.
K. Irie, K. Kitagawa, H. Nagura, T, Imai, T. Shimomura and Y. Fujiyoshi.
J. Biol. Chem., 285, 3685-3694 (2010).

119.

Structural and functional characterization of H+,K+-ATPase with bound fluorinated phosphate analogs.
K. Abe, K. Tani and Y. Fujiyoshi.
J. Struct. Biol., 170, 60-68 (2010).

120.

Unique multipotent cells in adult human mesenchymal cell populations.
Y. Kuroda, M. Kitada, S. Wakao, K. Nishikawa, Y. Tanimura, H. Makinoshima, M. Goda, H.
Akashi, A. Inutsuka, A. Niwa, T. Shigemoto, Y. Nabeshima, T. Nakahata, YI. Nabeshima, Y.
Fujiyoshi and M. Dezawa
PNAS, 107, 8639-8643 (2010).

121.

Evidence for lateral mobility of voltage sensors in prokaryotic voltage-gated sodium channels.

H. Nagura, K. Irie, T. Imai. T. Simomura, T. Hige and Y. Fujiyoshi
Biochem. Biophys. Res. Commun., 399, 341-346 (2010).
122.

Human Umbilical Cord-Derived Mesenchymal Stromal Cells Differentiate into Functional Schwann
Cells that Sustain Peripheral Nerve Regeneration.
D. Matsuse, M. Kitada, M. Kohama, K. Nishikawa, H. Makinoshima, S. Wakao, Y. Fujiyoshi, T.
Heike, T. Nakahata, H. Akutsu, A. Umezawa, H. Harigae, J. Kira and M. Dezawa
J. Neuropathol. Exp. Neurol., 69, 973-985 (2010).

123.

Influence of the Cytoplasmic Domains of Aquaporin-4 on Water Conduction and Array Formation.
T. Mitsuma, K. Tani, Y. Hiroaki, A. Kamegawa, H. Suzuki, H. Hibino, Y. Kurachi and Y.
Fujiyoshi
J. Mol. Biol., 402, 669-681 (2010).

124.

Asymmetric configurations and N-terminal rearrangements in connexin26 gap junction channels.
A. Oshima, K. Tani, M. M. Toloue, Y. Hiroaki, A. Smock, S. Inukai, A. Cone, B. J.
Nicholson, G. E. Sosinsky and Y. Fujiyoshi
J. Mol. Biol., 405, 724-735 (2011).

125.

Conformational rearrangement of gastric H+,K+-ATPase induced by an acid suppressant.
K. Abe, K. Tani and Y. Fujiyoshi
Nature Commun., 2, 155 pp1-7 (2011).

126.

Arrangement and mobility of the voltage sensor domain in prokaryotic voltage-gated sodium channels.
T. Simomura, K. Irie, H. Nagura, T. Imai and Y. Fujiyoshi
J. Biol. Chem., 286, 7409-7417 (2011).

127.

Water permeability and characterization of aquaporin-11.
K. Yakata, K. Tani and Y. Fujiyoshi
J. Struct. Biol., 174, 315-320 (2011).

128.

Electron tomographic analysis of gap junctions in the lateral giant fibers of crayfish.
Y. Ohta, K. Nishikawa, Y. Hiroaki and Y. Fujiyoshi
J. Struct. Biol., 175, 49-61 (2011).

129.

Integumental reddish-violet coloration owing to novel dichromatic chromatophores in the teleost fish,
Pseudochromis diadema.
M. Goda, M. Ohata, H. Ikoma, Y. Fujiyoshi, M. Sugimoto and R. Fujii.
Pigment Cell Melanoma Res., pp11-4 (2011).

130.

Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent
stem cells in human fibroblasts.
S. Wakao, M. Kitada, Y. Kuroda, T. Shigemoto, D. Matsuse, H. Akashi, Y. Tanimura, K.
Tsuchiyama, T. Kikuchi, M. Goda, T. Nakahata, Y. Fujiyoshi and M. Dezawa
PNAS, 108, 9875-9880 (2011).

131.

The carboxy-terminal helical bundle of the tetrameric prokaryotic sodium channel accelerates theinactivation rate.
K. Irie, T. Shimomura and Y. Fujiyoshi
Nature Commun., 3, 793 pp1-8 (2012).

132.

Gating movement of acetylcholine receptor caught by plunge-freezing.
N. Unwin and Y. Fujiyoshi
J. Mol. Biol., 422, 617-634 (2012).

133.

Cryo-EM structure of gastric H+,K+-ATPase with a single occupied cation binding site.
K. Abe, K. Tani T. Friedrich and Y. Fujiyoshi
PNAS, 109, 18401-18406 (2012).

134.

The four-transmembrane protein IP39 of Euglena forms strand by a trimeric unit repeat.
H. Suzuki, Y Ito, Y Yamazaki, K Mineta, M Uji, K. Abe, K. Tani, Y. Fujiyoshi and S.
TsukitaNature Commun., 4, 1766 pp1-8 (2013).

135.

Oligomeric structure and functional characterization of C. elegans innexin-6 gap junction channels.
A. Oshima, T Matsuzawa, K. Nishikawa and Y. Fujiyoshi
J. Biol. Chem., 288, 10513-10521 (2013).

136.

Novel dichromatic chromatophores in the integument of mandarin fish Synchiropus splendidus.
M. Goda, Y. Fujiyoshi, M. Sugimoto and R. Fujii
Biol. Bull., 224, 14-17 (2013).

137.

Ligand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95
K. Mintohara, S. Ichikawa, T. Seki, Y. Fujiyoshi and T. Doi
Neuroscience letters, 533, 44-49 (2013).

138.

Visualization of two distinct states of disassembly in the bacterial V-ATPase from Thermus
thermophilus.
K. Tani, C. P. Arthur, M. Tamakoshi, K. Yokoyama, K. Mitsuoka, Y. Fujiyoshi and C. Gerle
Microscopy, 62, 467-474 (2013).

139.

Two alternative conformations of a voltage-gated sodium channel.
C.J. Tsai, K. Tani, K. Irie, Y. Hiroaki, T. Shimomura, D.G. McMillan, G.M. Cook, G.F.X.
Schertler, Y. Fujiyoshi and X.D. Li
J. Mol. Biol., 425, 4074-4088 (2013).

140.

Carbon sandwich preparation preserves quality of two-dimensional crystals for cryo-electron
microscopy.
F. Yang, K. Abe, K. Tani and Y. Fujiyoshi
Microscopy, 62, 597-606 (2013).

141.

Moyamoya disease-associated protein mysterin/RNF213 Is a novel AAA+ ATPase, which dynamicallychanges its oligomeric state.
D. Morito, K. Nishikawa, J. Hoseki, A. Kitamura, Y. Kotani, K. Kiso, M. Kinjo, Y.
Fujiyoshi and K. Nagata
Scie. Rep., 4, 4442 (2014).

142.

Crystal structure of a Claudin provides insight into the architecture of tight junctions.
H. Suzuki, T. Nishizawa, K. Tani, Y. Yamazaki, A. Tamura, R. Ishitani, N. Dohmae, S.
Tsukita, O. Nureki and Y. Fujiyoshi

Science, 344, 304-307 (2014).
143.

Systematic Comparison of Molecular Conformations of H+,K+-ATPase Reveals an
Important Contribution of the A-M2 Linker for the Luminal Gating.
K. Abe, K. Tani and Y. Fujiyoshi
J. Biol. Chem., 289, 30590-30601(2014).

144.

Model for the architecture of claudin-based paracellular ion channels through tightjunctions.
H. Suzuki, K. Tani, A. Tamura, S. Tsukita and Y. Fujiyoshi
J. Mol. Biol., 427, 291-297(2015).

145.

Structural insight into tight junction disassembly by Clostridium perfringens
enterotoxin.
Y. Saitoh, H. Suzuki, K. Tani, K. Nishikawa, K. Irie, Y. Ogura, A.
Tamura, S. Tsukita and Y. Fujiyoshi
Science, 347, 775-778 (2015).

146.

Bovine F1Fo ATP synthase monomers bend the lipid bilayer in 2D membrane crystals.
C. Jiko, K.M. Davies, K. Shinzawa-Itoh, K. Tani, S. Maeda, D.J.
Mills, T. Tsukihara, Y. Fujiyoshi, W. Kuhlbrandt and C. Gerle
eLife, 4, e06119 (2015).

147.

An Intracellular Domain with a Novel Sequence Regulates Cell Surface Expression and
Synaptic Clustering of Leucine-Rich Repeat Transmembrane Proteins in Hippocampal
Neurons.
K. Minatohara, Y. Murata, Y. Fujiyoshi and T. Doi
J. Neurochem., 134, 618-628 (2015).

148.

GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM.
F. Hauer, C. Gerle, N. Fischer, A. Oshima, K. Shinzawa Itoh, S.
Shimada, K. Yokoyama, Y. Fujiyoshi and H. Stark
Structure, 23, 1769-1775 (2015).

149.

Control of Spontaneous Ca2+ Transients Is Critical for Neuronal Maturation in theDeveloping Neocortex.
Y. Bando, K. Irie, T. Shimomura, H. Umeshima, Y. Kushida, M.
Kengaku, Y. Fujiyoshi, T. Hirano and Y. Tagawa
Cereb. Cortex., 26, 106-117 (2016).

150.

Two-dimensional crystal structure of aquaporin-4 bound to the inhibitor acetazolamide.
A. Kamegawa, Y. Hiroaki, K. Tani and Y. Fujiyoshi
Microscopy, 65, 177-184 (2016).

151.

Characterization of physiologic phenotypes of dentate gyrus synapses of PDZ1/2
domain-deficient PSD-95 knockin mice.
H. Nagura, T. Doi and Y. Fujiyoshi
Eur. J. Neurosci., 43, 618-625 (2016).

152.

Claudin-21 has a paracellular channel role at tight junctions.
H. Tanaka, Y. Yamamoto, H. Kashihara, Y. Yamazaki, K. Tani, Y.
Fujiyoshi, K. Mineta, K. Takeuchi, A. Tamura and S. Tsukita
Mol. Cell Biol., 36, 954-964 (2016).

153.

Hexadecameric structure of an invertebrate gap junction channel.
A. Oshima, T. Matsuzawa, K. Murata, K. Tani and Y. Fujiyoshi
J. Mol. Biol., 428, 1227-1236 (2016).

154.

Thermostabilization of the human endothelin type-B receptor.
A. Okuta, K. Tani, S. Nishimura, Y. Fujiyoshi and T. Doi
J. Mol. Biol., 428, 2265-2274 (2016).

155.

Molecular determinants of prokaryotic voltage-gated sodium channels for recognitionof local anesthetics.
T. Shimomura, K. Irie and Y. Fujiyoshi
FEBS J., 283, 2881-2895 (2016).

156.

Activation mechanism of endothelin ETB receptor by endothelin-1.
W. Shihoya, T. Nishizawa, A. Okuta, K. Tani, N. Dohmae, Y.
Fujiyoshi, O. Nureki and T. Doi
Nature, 537, 363-368 (2016).

157.

Atomic structure of the innexin-6 gap junction channel determined by cryo-EM.
A. Oshima, K. Tani and Y. FujiyoshiNat. Commun., 7, 13681 pp1-8 (2016).

------------------------------------------------------------------------------(Reviews)
1.

The Structural Study of Membrane Proteins by Electron Crystallography.
Y. Fujiyoshi
Adv. Biophys., 35, 25-80 (1998).

2.

Electron Crystallography of Membrane proteins.
Y. Fujiyoshi
G protein-Coupled receptors (CRC Press: Edited by T. Haga and G. Berstein),323-354 (1999).

3.

The Importance of Aquaporin Water Channel Protein Structures.
A. Engel, Y. Fujiyoshi and P. Agre
EMBO J. 19, 800-806 (2000).

4.

Two-dimensional crystals: a powerful approach to assess structure, function anddynamics of membrane proteins.
H. Stahlberg, D. Fotiadis, S. Scheuring, H. Remigy, T. Braun, K.
Mitsuoka, Y. Fujiyoshi and A. Engel
FEBS Letters, 504, 166-172 (2001).

5.

Aquaporin water channels-from atomic structure to clinical medicine.
P. Agre, L. S. King, M. Yasui, W. B. Guggino, O. P. Ottersen, Y. Fujiyoshi, A.
Engel and S. Nielsen
Journal of Physiology, 542.1, 3-16 (2002).

6.

Structure and function of water channels.
Y. Fujiyoshi, K. Mitsuoka, B. L. de Groot, A. Philippsen, H. Grubmüller, P.

Agre and A. Engel
Current Opinion in Structural Biology, 12, 509-515 (2002).
7.

Structural Genomics of Membrane Proteins.
Y. Kyogoku, Y. Fujiyoshi, I. Shimada, H. Nakamura, T. Tsukihara, H.
Akutsu, T. Odahara, T. Okada and N. Nomura
Accounts of Chemical Research, 36, 199-206 (2003).

8.

Junction-forming aquaporins.
A. Engel, Y. Fujiyoshi, T. Gonen and T. Walz
Current Opinion in Structural Biology, 18, 229-235 (2008).

9.

Electron crystallography of proteins in membranes.
Y. Fujiyoshi and N. Unwin
Current Opinion in Structural Biology, 18, 587-592 (2008).

10.

The AQP structure and functional implications.
T. Walz, Y. Fujiyoshi and A. Engel
Handb. Exp. Pharmacol., 190, 31-56 (2009).

11.

Electron crystallography and aquaporins
A. D. Schenk, R. K. Hite, A. Engel, Y. Fujiyoshi and T. Walz
Methods in Enzymology, 483, 91-119 (2010).

12.

Structural physiology based on electron crystallography.
Y. Fujiyoshi
Protein Science, 20, 806-817 (2011).

13.

Electron crystallography for structural and functional studies of membrane proteins.
Y. Fujiyoshi
J. Electron microsc., 60 (Suppl. 1), S149-S159 (2011).

14.

Water channel structures analysed by electron crystallography.
K.Tani and Y. Fujiyoshi
Biochim. Biophys. Acta., 1840, 1605-1613 (2014).

15.

Development of the field of structural physiology.
Y. Fujiyoshi
Proc. Jpn. Acad. Ser. B. Phys. Biol. Sci., 91, 447-468 (2015).

16.

Cryo-electron microscopy for structure analyses of membrane proteins in the lipid
bilayer.
K. Abe and Y. FujiyoshiCur. Opin. Struct. Biol., 39, 71-78 (2016).

17.

Crystal structures of claudins: insights into their intermolecular interactions.
H. Suzuki, K. Tani and Y. Fujiyoshi
Ann. N. Y. Acad. Sci., (E-pub).