C18, C18-HT, RP-AQUA, C8, PhE, Biphenyl

HPLC column

Sunniest

TMS end-capping; PFP, PFP&C18
No end-capping; Cyano, Silica

ChromaNik Technologies Inc.

Unique end-capping by new conceptThis figure shows comparison of hydrophobicity between two C18 stationary phases. We
developed silanol activity control technique which was a reaction at extremely high temperature.
This technique makes residual silanol groups change to siloxane bond. The upper one is a C18
phase with conventional end-capping and the lower one is a C18 phase with both Sunniest endcapping and silanol activity control. A residual silanol group contributes as a polar site and makes
hydrophobicity of stationary phase decrease. On the other hand siloxane bond in the lower one
doesn’t make hydrophobicity decrease. Consequently the lower one is more hydrophobic than theupper one.

Conventional end-capping
C18

C18

C18

The whole works asstationary phase.
C18

C18

End-capping layer
Final TMSHOSi

Both Sunniest end-capping and Silanol Activity Control

O
Si

C18

C18

C18

C18

C18

More
Hydrophobic

End-capping layer

Si O
Si

O

Si

Si

O
Si
O

O
Si

O

Si
O

O

Si O
OO
Si
O

O

Si

Si
O
O

O

O O Si

Si
O
O OH
O
Si O
O
O
O
O
Si
Si
Si
HO
O O
OO O
O O
Si
O
Si
O
O
Si
Si
O O
Si O O OO Si O Si
O
O
O
O
Si

Si O

SiO

O

Si

O

Si

O

O

Si O

O O

Si

Si

Si

O

Schematic diagram of bonding of SunShell C18

State of stationary phase with unique end-capping andcomparison of hydrophobicity

An end-capping of hexamethyltrisiloxane works as anarm. This arm moves like a Geometrid caterpillar, sothat a functional group on the tip of the arm can bondwith a silanol group which is located anywhere.
Finally TMS reagent is bonded to a remaining silanolgroup.

Features★ Little residual silanol groups by an unique bonding technique★ Excellent stability, especially under acidic pH conditions★ Sharp peak shape for acidic, basic and chelating compounds★ RP-AQUA with C30 bonding and Biphenyl offer Performance in 100% aqueousconditions, and shows enhanced retention of polar compounds.
1

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Reversed phase
C18, C18-HT

PhE

O
O Si
O

O
O Si
O

Biphenyl

RP-AQUA, C30

O
O Si
O

O
O Si
O

PFP

C8,

F
F

F

O
O Si
O

O
O Si
O

F
F

PFP&C18

Cyano
O Si

O
O Si
O
O
O
O Si

CN

F
F

F
F
F

Normal phase & HILIC
SilicaO
O Si OHO

Characteristics of Sunniest
Particle size
(μm)

Pore diameter(nm)

Specific surface
area (m2/g)

Carbon content(%)

Bonded phaseEnd-capping

pH range

USP L
line

1.8, 3 and 5

12

340

16

C18

Sunniest end-capping

1.5 - 10

L1

Sunniest C18-HT

2

10

340

16

C18

Sunniest end-capping

1.5 - 10

L1

Sunniest RP-AQUA

3 and 5

12

340

16

C30

Sunniest end-capping

2-8

L62

Sunniest C18

Sunniest C8

3 and 5

12

340

10

C8

Sunniest end-capping

1.5 - 9

L7

Sunniest PhE

3 and 5

12

340

10

Phenylethyl

Sunniest end-capping

1.5 - 8

L11

Sunniest Biphenyl

5

12

340

11

Biphenyl

Sunniest end-capping

1.5 -9

L11

Sunniest PFP

5

12

340

10

Pentafluorophenyl

TMS end-capping

2-8

L43

Sunniest PFP&C18

5

12

340

14

PFP + C18

TMS end-capping

2-8

L43

Sunniest Cyano

5

12

340

5.5

Diisopropylcyanopropyl

No

2-8

L10

Sunniest Silica

3 and 5

12

340

0

Bare silica

No

1-5

L3

2

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica◆ Separation of standard samples
1 2

Sunrise C30

3

5

4

2 3

3

Sunniest RP-AQUA

1

5

7

5
3

4

2,3

7 5

1

7
6

4
2

7
6

4
2

7

6

5

Sunniest C18

1

Column: Sunrise C30,
Sunniest C18, RP-AQUA, C8, PhE,
Biphenyl, PFP, PFP&C18, Cyano
5 µm, 150 x 4.6 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil,
2 = Caffeine,
3 = Phenol,
4 = Butylbenzene,
5 = o-Terphenyl,
6 = Amylbenzene,
7 = Triphenylene.

Sunniest C8

6

Hydrogen bond Hydrophobicity
1

1 2

Sunniest Biphenyl5
3
1

4

4
1

7

Sunniest PFP

6

2,3

5

10

15

20

3

0

3

0.51

1.59

1.52

C18

0.43

1.56

1.37

RP-QUA

0.49

1.56

1.37

C8

0.33

1.43

1.23

PhE

1.00

1.34

0.92

Biphenyl

1.77

1.41

1.30

PFP

1.00

1.29

2.51

PFP&C18

1.00

1.43

2.66

Cyano

1.76

1.21

1.70

6
4

25

30

35

40

Retention time/min

5
2

C30

７

7
1

(Triphenylene/o-Terphenyl)

Sunniest PFP＆C18

5

6

4

0

7

6

5

2,3

(Amylbenzene/
Butylbenzene)

Sunniest PhE

4 6

Steric selectivity(Caffeine/
Phenol)Sunniest Cyano

2.5
Retention time/min

5

Nine types of stationary phases were compared hydrogen-bonding α (Caffeine / Phenol), hydrophobic α (Amylbenzene /Butylbenzene), and steric selectivity α (Triphenylene / o-Terphenyl). The hydrogen bonding expressed by the separation factor of
caffeine and phenol was lower in the alkyl group stationary phase with high temperature end-capping, but slightly higher in PhE(phenethyl group), PFP (pentafluorophenyl group), and PFP & C18. The elution times of caffeine and phenol were the same, andthe separation factor was 1. Biphenyl was subjected to high-temperature end-capping and was almost unaffected by residual
silanol groups, but the hydrogen-bonding value was 1.77, showing high hydrogen-bonding. This is a characteristic of Biphenyl,
and it is thought that it has a large hydrogen bond property due to the bonding of two benzene rings. Cyano was not end-capped,
and it is thought that the hydrogen bonding was increased due to the influence of the residual silanol groups. Hydrophobicity ishigher in the stationary phase with higher carbon content. The phenyl-based stationary phase is lower than the alkyl-based
stationary phase. PFP & C18 is a stationary phase with increased hydrophobicity of PFP, and both stationary phases have thesame hydrogen bonding property and steric selectivity, but only the hydrophobicity is changed. The steric selectivity tends toincrease as the alkyl group becomes longer, but PFP and PFP & C18 have extremely large values. And it is expected to show
specific separation. The elution order is different between the alkyl group stationary phase and the phenyl group stationary phase,and there is a large difference in separation selectivity.

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica◆ Evaluation of End-capping
Comparison of plates number (N) and USP tailing factor (TF) of amitriptyline
CH3OH, pH7.5, 40 ºC
Sunniest C18
1

4

N(4) =11,000
TF(4) =1.06

3
2

4

Sunniest RP-AQUA
1

Column size: 150 x 4.6 mm
Particle size: 5 µm
Mobile phase:
CH3OH/20mM Phosphate buffer pH7.5 or 6.0 =80/20
CH3CN/20mM Phosphate buffer pH7.0 =60/40
Flow rate: 1.0 mL/min
Temperature: 40 ºC or 22 ºC
Sample: 1 = Uracil
CH
2 = Propranolol
O
NH
CH

N(4) =10,500
TF(4) =1.07

3
2

3

3

OH

1

4

Company A C18
2

1

NH
CH3

4 = Amitriptyline
CH3

4Company B C18

N

N(4) =10,000
TF(4) =1.50

32

3 = Nortriptyline

N(4) =8,950
TF(4) =1.76

3

CH3

CH3CN, pH7.0, 40 ºC
0

2

4

6
8
10
12
Retention time/min

14

16

Sunniest C18

4

3

1
2

CH3OH, pH6.0, 22 ºC

Sunniest RP-AQUA
2

Sunniest C18
4

3

1

N(4) =9,000
TF(4) =1.09

2

N(4) =8,000
TF(4) =1.10

2Company A C18

4

3

0

2

4

Company B C18

4

6

8

4

3
2

N(4) =12,000
TF(4) =1.09

Sunniest C18

10

12

Retention time/min

14

N(4) =10,000 Sunniest PhE
TF(4) =1.14

2 3

1Company A C18

1

2

N(4) =4,000
TF(4) =4.33

Company B C18
3

2

0

4

3

2

N(4) =5,800
TF(4) =2.86

3

2

1

N(4) =3,900
TF(4) =2.92

2

1

1

4

3

1

Sunniest C8

4

Sunniest RP-AQUA
1

N(4) =12,400
TF(4) =1.10

4

3

1

N(4) =12,500
TF(4) =1.04

4

6

8

10

12

14

16

4

N(4) =4,300
TF(4) =5.24

18

20

22

24

Retention time/min

Amitriptyline is widely used to evaluate residual silanol groups on the C18 stationary phase. Peak shape of
Amitriptyline was compared under 3 kinds of conditions such as methanol/phosphate buffer/40 ºC,
methanol/phosphate buffer/22 ºC and acetonitrile/phosphate buffer/40 ºC. Majority of the HPLC columns
offered good peak shapes under methanol/phosphate buffer/40 ºC conditions. However using Mobile phaseof acetonitrile/phosphate buffer/40 ºC, most of the columns (Refer column A and B) offered high extent oftailing unlike Sunniest columns offering a symmetrical peak.
Sunniest C18, RP-AQUA and C8 columns allow to use a wide range of mobile phase without peak tailingbecause of extremely low content of residual silanol groups on the stationary phase.

4

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Relative plate number of butylbenzene (%)◆Stability under acidic and basic pH conditions

Relative retention of ethylbenzene (%)

100
90
80
70
60
Sunniest C18

50

Sunniest RP-AQUA
Sunniest C8

40

Sunniest PhE

30

B company C18
D compnay C18

20
0

20

40

60

80

100

120

120

100

80

60

Sunniest C18, pH10 – 50ºC

40

Sunniest C8, pH9.2 – 40ºC

20

0
0

200

600

800

Time (h)Time (h)）

Durable test condition400

Durable test condition
Column: Sunniest C18, C8, 5 µm 150 x 4.6 mm

Column size: 150 x 4.6 mm

Mobile phase:

Mobile phase: CH3CN/1.0% TFA (pH1) = 10/90

C18: CH3OH/20mM Sodium borate/10mM NaOH=30/21/49

Flow rate: 1.0 mL/min(pH10)

Temperature: 80 ºC

C8: CH3OH/20mM Sodium borate (pH9.2) =30/70
Flow rate: 1.0 mL/min

Measurement condition

Temperature: C18 - 50 ºC, C8 - 40 ºC
Measurement condition

Column size: 150 x 4.6 mm
Mobile phase: CH3CN/H2O=60/40
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil
2 = Ethylbenzene

Stability under acidic pH conditions was evaluated at 80 ºC using
acetonitrile/1% trifluoroacetic acid solution (10:90) as mobile
phase. 100% aqueous mobile phase expels from the pore of
packing materials by capillarity and packing materials don’t
deteriorate. 10% acetonitrile in a mobile phase allows an
accurate evaluation.1-3)★Sunniest C18 has kept 90% retention for 100 hours
under severe conditions of acetonitrile /1% trifluoroacetic
acid solution (pH 1)at 80 deg C.
Our Unique bonding technique offers significant
enhancement of column life,
Considering the Sunniest RP-AQUA C30 ligand length theSunniest RP-AQUA is less stable than Sunniest C18.
However, Sunniest RP-AQUA C30 column with hightemparature bonding along with end capping offers longer
column life in comparison to other RP Aqua columns.

51) N. Nagae, T. Enami and S. Doshi, LC/GC North America October 2002.
２) T. Enami and N. Nagae, American Laboratory October 2004.
３) T. Enami and N. Nagae, BUNSEKI KAGAKU, 53 (2004) 1309.

Column: Sunniest C18, C8, 5µm 150 x 4.6 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Butylbenzene

Stability under basic pH conditions was evaluated at 50 ºC using
methanol/Sodium borate buffer pH 10 (30:70) as a mobile phase.
Sodium borate is used as a alkaline standard solution for pH meter,
so that its buffer capacity is high.
Elevated temperature of 10 ºC makes column life be one third. When
Sunniest C18 column is used at 40 ºC, column life becomes 2,000
hours. Most of the HPLC columns stability data is offered atambient room temperature alternate 25 ºC at pH 1-10 units. At
temperature of 25 ºC, the column life is sixteen times longer thanthat at 50 ºC.

★ Sunniest C18 offers performance at elevated pH andtemperature. Regarding stability under basic pH condition,
there are very few C18 column like Sunniest C18 & Hybrid
type C18 which can sustain and offer performance under suchchallenging conditions of high temperature and pH. It isconsidered that our double end-capping & base deactivation
technique leads higher stability.
★ Sunniest C18 has operational pH Range from 1.5 to 10.
Sunniest C8,Phenyl has operational pH Range 1.5 to 9 andSunniest RP-Aqua and Pentafluorophenyl (PFP) at pH 2-8..

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest C18◆ Relationship between pH and retention of Acidic, Basic and Neutral compounds
B

N

Retention factor (k)塩基性化合物 (B)pH2A

中性化合物 (N)A

酸性化合物 (A)

A

N
B

pH7

N
B

pH10

pH
Ionic compounds are promoted or suppressed in ionization by the mobile phase pH. The higher the pH of the mobile phase for acidic compounds andthe lower the pH of the mobile phase for basic compounds, the more ionization is promoted and the higher the polarity, so the retention is smaller.

◆ pH selectivity
2 3

Column: Sunniest C18, 5 µm, 150 x 4.6 mm

1Mobile phase: A1) 20 mM Phosphoric acid pH 2.3

pH2.3

6

5

4

A2) 20 mM Phosphate buffer pH 7
A3) 20 mM Phosphate buffer pH 10

3
1
4 2

5

B) Acetonitrile

pH7

6

3,5
2

30

2

26

UV@250 nm, Sample: 1= Thiamine HCl Vitamin B1, 2 =

6

1

0

%B (%)

Flow rate: 1.0 mL/min, Temperature: 40 ºC, Detection:

pH10

4

Time (min)Nicotinamide, 3 = Nicotinic acid, 4 = Pyridoxine HCl
0

5

10

15
Retention time/min

20

25

Vitamin B30
6, 5 = Folic acid, 6 = Riboflavin Vitamin B2◆ Separation of local anesthetic and effect by pH of mobilr phase
Column: Sunniest C18 5 µm, 150 x 4.6 mm

Methanol/20 mM Phosphate buffer (pH 7.0)=70/30
2

Mobile phase: Shown in the figure

3

1

4

5

Flow rate : 1.0 mL/min
Pressure : 10.4 MPa
Temperature : 40 oC
Detection : UV@250nm

Methanol/20 mM Ammonium acetate buffer (pH 6.8)=70/30
3

2

5

1

Injection volume : 1 µL
Sample : 1 = Benzocaine,

4

N
CH 2
HO

H
H

2 = Procaine,
Methanol/20 mM Ammonium bicarbonate buffer (pH 9.5)=70/30
2
3
5
1
4

H

H
N

3 = Cinchonine,

4 = Lidocaine,
0

2

4

6

8

10

5 = Tetracaine

Retention time /min
Separation using a neutral phosphate buffer may not be reproducible if changed to ammonium acetate salt for LC / MS, but separation can be improvedby adjusting to alkaline with ammonium bicarbonate buffer. This is the case for the above separation of local anesthetics.
In the analysis of ionic compounds, pH can significantly change the separation selectivity. Since the Sunniest C18 column has highly stable under basic
pH condition and can use an alkaline mobile phase with a pH of 10, the mobile phase conditions can be changed significantly from acidic to alkaline, andthe optimum analytical method can be established.

6

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest C18◆ Comparison data: Bleeding from column《Comparison using MS》《Comparison using Corona CAD》

5.E+085.E+08Company F C18
Area: 960,000

4.E+08Company B C18

Intensity(cps)
Intensity (cps)

4.E+08Company B C18
Area: 1,150,000

3.E+083.E+082.E+082.E+081.E+08Sunniest C18

Area: 94,000

Sunniest C18

5.E+070.E+00
0

5

10

15

20

Time (min)Retention time/min

Column size: 150 x 2.0 mm
Mobile phase:A) 0.1% acetic acidB) CH3CN
Gradient: Time: 0min 3min 14.4min 18min 19min%B: 5% 5% 100% 100% 5%
Flow rate: 0.2 mL/min
Temperature: 40 ºC
MS： ABI API-4000
Ionization: Turboionspray (cation)
Measurement mode: Q1 Scan m/z 100-1000

Column size: 150 x 2.0 mm
Mobile phase:A) 0.1% acetic acidB) CH3CN
Gradient: Time: 0min 3min 14.4min 18min 19min%B: 5% 5% 100% 100% 5%
Flow rate: 0.2 mL/min
Temperature: 40 ºC
Detection: Corona CAD◆ Separation of antidepressants using Acetonitrile and Ammonium acetate for LC/MS
N=12,200
TF=1.30
1

N=12,100
TF=1.21

N=12,000
TF=1.28

Sunniest C18
Sunniest RP-AQUA
N=5,100
TF=2.4

Sunniest C8
N=8,500
TF=2.6Company E C18

N=5,500 TF=2.1

Company F C18

CH3

N=8,100 TF=2.0

Company G C18

2

4

6

8

10

12

Retention time/min

7

N
CH3

Company H C18
0

Column size: 150 x 4.6 mm
Particle size: 5 μm
Mobile phase: CH3CN/10mM
Ammonium acetate pH6.8=40/60
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: Amitriptyline

14Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest RP-AQUA◆ Reproducibility of retention under 100% aqueous conditions★ C18 and C8 reversed phases exhibit decreased and poorly
reproducible retention under more than 98% aqueous conditions asshown in Fig. 1. This problem traditionally has been explained as
being the result of ligand collapse or a matting effect. Nagae1-3
ascertained , however , that the mobile phase was being expelledfrom the pores of the packing material under 100% aqueous mobile
phase conditions, as Fig. 2 shows.

After stopping pump for 1 hour

★ When the surface of packing materials isn’t wet by water, waterused as a mobile phase expels from the pore of the packing
material by capillarity. This is a reason why reproducibility inretention is low under 100% aqueous conditions. Reverselypressure around the packing material makes water permeate into
the pore of the packing material to overcome a force worked bycapillarity.

N. Nagae, T. Enami and S. Doshi, LC/GC North America October 2002.

Fig. 1 Retention behavior of a C18
column under 100% aqueous mobile
phase conditions

Water permeates
into the pore.

In other words, the surface of a reversed phase like C18 isn’t wet
by water anytime even if water permeates into the pore of thepacking material or not. So it is wrong that we say “ dewetting”
when water expel from the pore. Saying “ Depermeatiing” is more
appropriate.

Permeating
SilicaPressure★ Sunniest RP-AQUA /C30 is a reversed stationary phase, so that
it is not wet with water. However the contact angle of water on thesurface of Sunniest RP-AQUA /C30 is less than that of aconventional C18. Expelling force (pressure) acted by capillarity
on Sunniest RP-AQUA /C30 is less than atmospheric pressure. So,
Sunniest RP-AQUA /C30 shows reproducible retention under
100% aqueous conditions.

Stop flow
Water

Water expels from thepore by capillarity.

Depermenating
10nm

Fig. 2 Schematic diagram of C18 particle２) T. Enami and N. Nagae, American Laboratory October 2004.

1) N. Nagae, T. Enami and S. Doshi, LC/GC North America October 2002.
３) T. Enami and N. Nagae, BUNSEKI KAGAKU, 53 (2004) 1309.

◆ Separation of nucleic acid bases

1

2

5
3
4

1

5

2

25 ºC

3
4

0

2

4

6

8

10

12

14

Retention time/min

Relative retention of thymine (%)

40 ºCChange of retention of thymine at 40 ºC(measurement every stop flow for 1 hour)
100
90
80
70
60
50
40
30
20
10
0
1

Column: Sunniest RP-AQUA,/C28 5µm 150 x 4.6 mm
Mobile phase: 10 mM Phosphate buffer pH 7.0
Flow rate: 1.0 mL/min
Temperature: 40 ºC and 25ºC
Sample: 1 = Cytosine 2 = Uracil
3 = Thymidine 4 = Uridine
5 = Thymine

2

3

4

5

Measurement number ev ery stop flow

Sunniest RP-AQUA/C30 showed more than 97% of reproducibility
in retention using 100% aqueous buffer as a mobile phase.

8Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest RP-AQUA

Basic pH test

Acidic pH test
100

Relative plate number of thtmine (%)

Relative retention of ethylbenzene (%)◆ Stability of Sunniest RP-AQUA/ C30 under 100% aqueous conditions

90
80
70
60
50
40

Sunniest RP-AQUA/C28

30
20

100
90
80
70
60
50

Sunniest RP-AQUA

40
30
20

0

20

40

60

80

100

120

140

0

100

200

300

400

Time (h)Time (h)Durable test conditions

Durable test conditions

Column: Sunniest RP-AQUA, 5µm 150 x 4.6 mm
Mobile phase: 0.5% TFA
Flow rate: 1.0 mL/min
Temperature: 60 ºC

Column: Sunniest RP-AQUA, 5µm 150 x 4.6 mm
Mobile phase: 20mM Phosphate buffer pH8.0
Flow rate: 1.0 mL/min
Temperature: 40 ºC

Measurement conditions

Measurement conditions

Column: Sunniest RP-AQUA, 5µm 150 x 4.6 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil
2 = Amylbenzene

Column: Sunniest RP-AQUA, 5µm 150 x 4.6 mm
Mobile phase: 10mM Phosphate buffer pH7.0
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Thymine

It is important that stability is evaluated for RP-AQUA columns under 100% aqueous conditions because RP-AQUA column life
becomes longer with incremental contents of organic solvent in a mobile phase. Sunniest RP-AQUA/C30 column can be used
under 100% aqueous conditions from pH 2 to pH 8.
★Sunniest RP-AQUA/C30 column can be used under 100% aqueous conditions from pH 2 to pH 8. Sunniest RP-AQUA/C30 is
one of the most stable aqua type column.
★ Sunniest RP-AQUA/C30 column with high temperature bonding along with end capping offers longer column life incomparison to other RP Aqua columns

9Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest RP-AQUA◆ Separation of water-soluble vitamins
1◆ Separation of organic acids
8

4

2

3

2 3
4

11

1
5

0

1

2

3

4

5

6

7

8

9

10 11 12 13 14

0

1

2

7

6

9
10

3

4

5

6

7

8

Retention time/min

Retention time/min

Column: Sunniest RP-AQUA, 5 µm 150 x 4.6 mm

Column: Sunniest RP-AQUA, 3 µm 150 x 4.6 mm

Mobile phase: 40 mM Phosphate buffer pH 6.8

Mobile phase: 25 mM H3PO4 pH 2.5

Flow rate: 1.0 mL/min

Flow rate: 1.0 mL/min Temperature: 25 ºC

Temperature: 40 ºC

Detection: UV@210 nm

Detection: UV@250 nm

Sample: 1 = Oxalic acid, 2 = Tartaric acid, 3 = Formic acid,

Sample: 1 = Nicotinic acid, 2 = Pyridoxal

4 = Malic acid, 5 = Lactic acid, 6 = Acetic acid,
7 = Diglycolic acid, 8 = Maleic acid, 9 = Citric acid,

3 = Pyridoxine, 4 = Nicotinamide

10 = Succinic acid, 11 = Fumaric acid

◆ Separation of water-soluble vitamins using
mobile phase for LC/MS◆ Separation of Oolong tea
カフェイン
Caffeine

1

3

Guanilic
acid
グアニル酸

シュウ酸
Oxalicacid

4

Theanine
テアニン

3

2
5

4
1

6
5

2

0

5

10

15

20

25

30

35

40

45

50 55

0

2

4

6

8

10

Retention time/min

12

14

16

18

20

Column: Sunniest RP-AQUA 5 µm, 250 x 4.6 mm

Column: Sunniest RP-AQUA 5 µm, 150 x 4.6 mm

Mobile phase: A) H2O/H3PO4 (99.9:0.1)

Mobile phase: A) 20mM Ammonium acetateB) CH3CN

2426B) Acetonitrile/ A solution (20:80)

Time (min)

0

20

60

Time (min)

0

5

20

25

%B (%)0050%B (%)

0

0

100

100

Flow rate: 1.0 mL/min

Flow rate: 1.0 mL/min

Temperature: 40 ºC

Temperature: 40 ºC

Detection: UV@210 nm

Detection: UV@250 nm

Injection volume: 10 µL
Sample: Oolong tea

22

Retention time/min

Sample: 1= Nicotinic acid
1 = (-)-Epigallocatechin

2 = Pyridoxine HCl Vitamin B6

2 = (+)-Catechin

3 = Nicotinamide

3 = (-)-Epigallocatechin gallate

4 = Thiamine HCl Vitamin B1

4 = (-)-Epicatechin

5 = Folic acid

5 = (-)-Epicatechin gallate

6 = Riboflavin Vitamin B2

10

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest PFP&C18◆ Comparison of retention time1

7Sunniest PFP

6

4
1

Sunniest PFP＆C18

2,3
4

11

2
3

Sunniest C18

5

10

7

5

6

4

0

Fluorobenzenes
フルオロベンゼン類の分離Standard
samples
標準試料の分離
2,3 5

5

7

6

15

20

25

30

35

40

Retention time/min
Column dimension: 150 x 4.6 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene

Hydrogen bondHydrophobicity

Steric selectivity(Caffeine/Phenol)(Amylbenzene/Butylbenzene)(Triphenylene/o-Terphenyl)

Column: SunShell PFP 2.6 µm, 150 x 4.6 mm
SunShell PFP&C18 2.6 µm, 150 x 4.6 mm
SunShell C18 2.6 µm, 150 x 4.6 mm
Mobile phase: Methanol/water=60/40
Flow rate: 1.0 mL / minTemperature: 40 oC
Detection: UV@250 nm
Sample:1 = Benzene, 2 = Fluorobenzene, 3 = Toluene, 4 = α,α,αTrifluorotoluene
F

PFP
PFP&C18
C18

1.00
1.00
0.43

1.29
1.37
1.56

2.51
2.66
1.37

CH 3

F

F
F

Compared to PFP, PFP&C18 has the same stereoselectivity, but its hydrophobicity is significantly increased and its retention
time is longer. As for the separation unique to PFP, such as the separation of fluorobenzenes, PFP&C18 has achieved thesame separation.

Relative plate number◆ Evaluation of Stability

Durable test condition
Column:Sunniest PFP&C18 5 µm, 150 x 2.0 mm
Sunniest PFP 5 µm, 150 x 2.0 mm
Mobil phase:
CH3OH/20 mM PB pH 7.0=70/30
Flow rate: 0.2 mL/min
Temperature: 40 ºC

3 timesPFP

PFP＆C18

Measurement condition
Column:PFP&C18 5 µm, 150 x 2.0 mm
PFP 5 µm, 150 x 2.0 mm
Mobil phase:
PFP&C18;
Acetonitrile/water=70/30
PFP;
Acetonitrile/water=60/40
Flow rate: 0.2 mL/min
Temperature: 40 ºC
Sample: Acenaphthene

Due to the increased hydrophobicity of the surface, PFP & C18
is about three times more durable than PFP.
Elution tie/h11

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest PFP&C18
Sunniest PFP◆ Comparison of highly polar compounds
1

２
Column: Sunniest PFP&C18, PFP 5 µm, 150 x 4.6 mm
Mobile phase: 12.5 mM Ammonium Formate (pH3)
Flow rate: 1.0 mL/min
Temperature: 25 ºC
Detection: UV@260 nm
Sample: 1 = Epinephrine, 2 = Levodopa

Sunniest PFP＆C18

２

1

1

2

Sunniest PFP

OH

H
N

O
HO
OH

HO

HO

NH2

OH

Levodopa

Epinephrine

Due to the increased hydrophobicity of the surface, PFP & C18 is about three times more durable than conventional PFP.
Separation of highly polar compounds such as catecholamines significantly changes the selectivity of PFP and PFP&C18,
with PFP tending to retain more.。

◆ Retention comparison between C18 and PFP
Sunniest C18
1

3
2

Column size: 150 x 4.6 mm
4 Mobile phase: CH3CN/20mM Phosphate buffer pH7.0 =60/40
Flow rate: 1.0 mL/min
Sample: 1=Uracil, 2=Propranolol, 3=Nortriptyline, 4=Amitriptyline

13Sunniest PFP

2★PFP retains a cation such a
nortriptyline much longer than a C18.
Retention time/min◆ Separation of Benzalkonium chloride

Sunniest Cyano

0

5

10
15
Retention time/min

20

25

Column: Sunniest Cyano 5 µm, 150 x 4.6 mm
Mobile phase: Methanol/50 m ammonium acetate=70/30
Flow rate: 1.0 mL/min
Temperature: 30 oC
Detection: UV@265 nm
Sample: Benzalkonium chloride

12

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest Biphenyl◆ Separation of amitriptyline◆ Separation of a chelating compound

Company A
BiphenylCompany A
BiphenylCompany B
BiphenylCompany B
BiphenylCompany C
BiphenylCompany C
Biphenyl

Sunniest Biphenyl

Sunniest Biphenyl

Column dimension: 250 x 4.6 mm
Mobile phase: CH3CN/20 mM Phosphate buffer pH 7.0=60/40
Flow rate: 2.0 mL/min
Temperature: 40 ºC
Detection: UV@250 nm
Sample: 1 = Uracil, 2 = Propranolol, 3 = Nortriptyline, 4 = Amitriptyline

Column dimension: 250 x 4.6 mm
Mobile phase: CH3CN/20mM H3PO4=10/90
Flow rate: 2.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = 8-Quinolinol (Oxine), 2 = Caffeine
O
H3C

CH 3
O

NH

CH 3

CH 3

NH

N
CH 3

OH

N

CH 3

O

OH

CH3
N

N
N

N

CH3

◆ Comparison of stability
Comparison of plate number under
weakly alkaline condition

Bleed comparison under acidic condition
80

100

60

━

50

━
━

40

━

30

Relative plate number/%

Absorbance/mAU

70

Sunniest Biphenyl
Company A Biphenyl
Company B Biphenyl
Company C Biphenyl

20

Chromatogram of a base line
at the third gradient cycle

10

80
60
●

Sunniest Biphenyl■ Company A Biphenyl
Company B Biphenyl▲ Company C Biphenyl

40
20

0

0

-10
5

6

7

8

9

10

11

Retention time/min

%B

0
10

Flow rate: 0.3 mL/min
Temperature: 40 ºC
Detection: UV@250 nm

13

5
10

10
90

0

50

100

150

200

250

300

Elution time/h

Bleed test condition
Column dimension: 50 x 2.1 mm
Mobile phase: A) 1% H3PO4 (pH 1.2)
B) Acetonitrile
Gradient programTime (min)12

Elution condition of stability test
Column dimension: 50 x 2.1 mm
Mobile phase: 20 mM Sodium phosphate pH 8.0
Flow rate: 0.2 mL/min
Temperature: 40 ºC
17
90

18
10

35
10

Measurement condition of plate number
Mobile phase: CH3CN/H2O=50/50
Flow rate: 0.2 mL/min
Temperature: 40 ºC
Detection: UV@250 nm
Sample: Butylbenzene

Stability under acidic condition compared baseline changes with gradient elution of 1% aqueous phosphate solution and acetonitrile. Biphenyl groups are cut off fromthe silica surface under acidic condition and elute out of the column as the amount of organic solvent increases. Baseline variability detected cut Biphenyl groups, with
Sunniest showing the least desorbed and high acid stability. In addition, under weakly alkaline condition, the silica dissolved and the column-in side was dented, so thetheoretical plate number of the columns were compared. Since the Biphenyl column has high reproducibility of retention time even in a 100% aqueous mobile phase(see page 14) and is effective for separating highly polar compounds, stability comparison was performed under the condition of pH 8 that does not contain an organic
solvent. Sunniest is more than twice as durable as other companies' columns.

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest Biphenyl◆ Comparison of stationary phases using nucleic acid bases
A) Methanol/10 mM ammonium acetate
pH6.8=20/80

2B) 10 mM ammonium acetate pH6.8

32

k6=0.6823

k5=0.58
6

3

C18

R5,6=1.0

5After stopping pump for1 hour
Decrease in retention: to 15%4

k6=4.53

0

1

2
2

0

3

2
2

3

4

6

8

2

1. NaNO2
2. Cytosine
NH 2N

3 After stopping pump for1 hour

6O

Unchanging rate in retention: 99%

k5=0.37

5

0

4

1

4

1

6

5
1

6
5

RP-AQUAR5,6=2.5

3. Uracil4O

k6=4.47
5

NH

6

4

1

1

N
H

N
H

O

4. Guanine
O

0

1

2
2

3

0

2
2

3,4
6

k5=0.76
5 R5,6=3.6

4

6

8

H
N

HN
H 2N

3

After stopping pump for1 hour
Unchanging rate in retention: 96%

BiphenylN

N

5. Thymine
O

6

H 3C

NH
N
H

5

4

1

1

k5=5.00

O

6. Adenine
N H2

0

1
2
Retention time/min

3

0

2

4
6
Retention time/min

8

N

HN
N

N

◆ Comparison of organic acid14

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest C18, C18-HT

Features✓ The 1.8 µm particles use a silica gel with the same physical characteristics as the 3 µm and 5 µm particles, and there isno difference in separation depending on the particle size.
✓ High-pressure packing achieves 100 MPa of a maximum operating pressure for 1.8 µm particles and 70 MPa of a
maximum operating pressure for 2 µm particles.
✓ Low pressure and high theoretical plate by precision classification✓ The inertness due to the Sunniest end-capping sharpens all peaks and leads usage of a wide pH range (pH 1.5 to 10).
✓ 2 µm Sunniest C18-HT has a 25% lower column pressure than 1.8 µm Sunniest C18.

★Unique surface modification (Comparison of amitriptyline as a basic compound）
2 3

4
Sunniest C18, 1.8 μm

N4=20,367
TF4=1.13

Column dimension: 100 x 2.1 mm
Mobile phase: CH3CN/20 mM phosphate buffer pH 7.0=60/40

1Flow rate: 0.3 mL/min
Temperature: 40 ºC
2 3

4

NH

Sample: 1 = Uracil,

N4=20,902
TF4=1.19

Sunniest C18-HT, 2 μm

1O

Detection: UV@250 nm

N
H

O

CH3

2 = Propranolol,
2
1

O

4
3

NH

N4=7,440 Company S
TF4=3.34 Core Shell C18, 2 μm
3 = Nortriptyline,

NH

4

2

1

3

N4=10,157
TF4=3.04

1

0

CH3

4 = Amitriptyline
N4=18,722
TF4=1.15

Company W C18, 1.7 μm

3Company S C18, 1.9 μm

2

2

CH 3

Company P
Core Shell C18, 1.7 μm

4

23

1

CH3

OH

4

6

N4=6,347 4
TF4=4.69
8NCH3＊吸着による保持増加
1012Retention time/min

Specification of Sunniest C18, 1.8 μm (Sunniest C18 shows the same characteristics for 1.8, 3 and 5 μm.)
Packings

Particle size
(µm)

Pore
diameter(nm)

Specific surface
area (m2/g)

Carbon loading
(%)

Phase

End-capping

Sunniest C18

1.8, 3 and 5

12

340

16

C18

High temperature reaction

1.5 - 10

L1

Sunniest C18-HT

2

10

340

16

C18

High temperature reaction

1.5 - 10

L1

Available pH
USP categoryrange

15 ＊Maximum operating pressure is 100 MPa for Sunniest C18, 1.8 μm and 70 MPa for Sunniest C18-HT 2 μm.

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Sunniest C18-HT★ Separation of Analgesics
Sunniest C18 5 µm, 150 x 4.6 mm

1Mobile phase: CH3CN/0.1% Formic acid = 20/80
Flow rate: 1.0 mL/min for 150 x 4.6 mm
0.6 mL/min for 50 x 2.1 mm
Temperature: 40 ºC
Detection: UV@230 nm
Sample:1=Acetaminophen, 2=Antipyrine, 3=Aspirin, 4=Ethenzamide

N(4)=80,000 plate/m
3
4

2

CH 3

NH

CH3

O

O

O

N
N

CH 3

O

OH

H 2N

O

H 3C

HO

O

O

CH 3

High throughput
N(4)=175,000 plate/m

2 μm particle allows to reduce retention time because highefficiency is kept under high flow rate conditions. As shown theabove chromatograms, analytical time reduced 1/8 without
sacrifices of separation by using 2 μm, 50 x 2.1 mm column
instead of 5 μm 150 x 4.6 mm column.

Sunniest C18-HT 2 µm, 50 x 2.1 mm

0

1

2

3

4

5

6

7

8

9

10 12

Retention time (min)★ Separation of Amino Acids derivatized with OPA
1

10

78

5

13

15 16
14

Column: Sunniest C18-HT 2 µm, 100 x 2.1 mm
Mobile phase: A) 10mM Na2PO4 + 10mM Na2B4O7 + 0.5mM NaN3
B) Acetonitrile/Methanol/Water (45/45/10 %V)

6

11
4
3
2

17
12
9

0

1

2

3

4

5

6

7

8

Time(min)

0

0.2

7.2

7.8

%B

5

5

50

100

Flow rate: 0.72 mL/min
Temperature: 40 ºC
Detection: UV@338 nm
Sample: 1=Aspartic acid, 2=Glutaminc acid, 3=Serine, 4=Histidine,
5=Glycine, 6=Threonine, 7=Arginine, 8=Alanine, 9=Tyrosine, 10=Valine,
11=Methionine, 12=Tryptophan, 13=Pnehylalanine, 14=Isoleucine,
15=Leucine, 16=Lysine, 17=Proline

9

Retention time (min)★ Guard column for Sunniest C18 1.8 µm, C18-HT 2 µm
SunShell guard cartridge column is recommended for Sunniest C18 µm, 2 µm.

⋆ The cartridge column is packed with SunShell C18 (RP) andHex 12 mm

Core shell silica (S) into a cartridge sized 3 x 2 mm i.d.

Holder⋆ RP guard cartridge is used for all reversed phases and S
guard cartridge for hilic phases.
Cartridge
2.0 mm I.D.
3 mm length

Tubing0.13 mm I.D.
50 mm length⋆ Low dead volume structure⋆ Upper pressure limit is more than 60 Mpa⋆ Availablr for 2.1 mm i.d. to 4.6 mm i.d. columns

Ordering Information of SunShell Guard Cartridge Column
Description

Part number

SunShell Guard Cartridge RP Starter Kit (holder, cartridge, tubing)

CB32CK

SunShell Guard Cartridge RP for exchange (2 PCS)

CB32CCSunShell Guard Cartridge S Starter Kit (holder, cartridge, tubing)

CS32CK

SunShell Guard Cartridge S for exchange (2 PCS)

CS32CCSunShell Guard Cartridge holder

HOL2CC

16

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica

Guard Cartridge (10 x 4 mm)
Feature*Simple structure*Low dead volume*Available for not
only 5 µm column
but also 3 µm
columnPEEK O-ring

FilterSchematic Diagram of
Cartridge 10x4mm

Photo of Cartridge and Holder

Comparison of chromatograms
Analytical column
only

N(4) =10,690
TF(4) =1.05

Column: Sunniest C18, 5 µm 150 x 4.6 mm
Guard cartridge 10 x 4 mm
Mobile phase:

Both Guard and Analyticalcolumns

0

17

1

2

3

4

N(4) =10,770
TF(4) =1.07

5 6 7 8 9 10 11
Retention time/min

CH3OH/20mM Phosphate buffer pH7.5 = 80/20
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Pressure: 4.8 MPa, 5.6 MPa(+guard)
Sample: 1 = Uracil,
2 = Propranolol,
3 = Nortriptyline,
4 = Amitriptyline,
TF: USP tailing factor

Particle size

Catalog No.

Sunniest C18, 5 µm Guard cartridge column (1-pak + Holder) 4 x 10 mm

5 µm

EB3A1H

Sunniest RP-AQUA, 5 µm Guard cartridge column (1-pak + Holder) 4 x 10 mm

5 µm

ER3A1H

Sunniest C8, 5 µm Guard cartridge column (1-pak + Holder ) 4 x 10 mm

5 µm

EC3A1H

Sunniest C18, 5 µm Guard cartridge (4-pak) 4 x 10 mm

5 µm

EB3A1C

Sunniest RP-AQUA, 5 µm Guard cartridge (4-pak) 4 x 10 mm

5 µm

ER3A1C

Sunniest C8, 5 µm Guard cartridge (4-pak) 4 x 10 mm

5 µm

EC3A1C

Sunniest Guard cartridge holder 4 x 10 mm---

HOLA1CSunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica✻ Sunniest Ordering information
Packings

Sunniest C18 1.8 µm

Sunniest C18-HT 2 µm
Packings

Sunniest C18 3 µm

Sunniest C18 5 µm

Sunniest RP-AQUA
3 µm
Sunniest RP-AQUA
5 µm

Sunniest C8 3 µm

Sunniest C8 5 µm

Sunniest PhE 3 µm

Sunniest PhE 5 µm

Sunniest Biphenyl 5 µm

Sunniest PFP 5 µm
Sunniest PFP&C18
5 µm
Sunniest Cyano 5 µm

Sunniest Silica 3 µm

Sunniest Silica 5 µm

Inner diameter
(mm)

2.1

3.0

4.6

10

20

Length (mm)Catalog number

Catalog number

Catalog number

Catalog number

Catalog number

30
50
75
100
150
30
50
75
100
Inner diameter(mm)

EBA931
EBA941
EBA951
EBA961
EBA971
EB1931
EB1941
EB1951
EB1961-----EBA341
EBA351
EBA361
EBA371
EB1331
EB1341-----EB1361------------------------------------------------------------------------------------------------------------------------------------------

2.0

3.0

4.6

10

20

Length (mm)Catalog number

Catalog number

Catalog number

Catalog number

Catalog number

50
75
100
150
250
50
100
150
250
50
75
100
150
250
50
100
150
250
50
75
100
150
250
50
100
150
250
50
75
100
150
250
50
100
150
250
50
100
150
250
50
100
150
250
50
100
150
250
50
100
150
250
50
75
100
150
250
50
100
150
250

EB2241EB2251EB2261
EB2271
EB2281
EB3241
EB3261
EB3271
EB3281
ER2241
ER2251
ER2261
ER2271
ER2281
ER3241
ER3261
ER3271
ER3281EC2241EC2251EC2261
EC2271
EC2281
EC3241
EC3261
EC3271
EC3281EP2241
EP2251
EP2261
EP2271
EP2281
EP3241
EP3261
EP3271
EP3281
E83241
E83261
E83271
E83281--------------------EV3241
EV3261
EV3271
EV3281
EJ3241
EJ3261
EJ3271
EJ3281ES2241ES2251ES2261
ES2271ES2281
ES3241
ES3261
ES3271
ES3281

EB2341-----EB2361
EB2371
EB2381
EB3341
EB3361
EB3371
EB3381
ER2341-----ER2361
ER2371
ER2381
ER3341
ER3361
ER3371
ER3381
EC2341-----EC2361
EC2371
EC2381EC3341EC3361
EC3371
EC3381
EP2341-----EP2361
EP2371
EP2381
EP3341
EP3361
EP3371
EP3381
E83341
E83361
E83371
E83381--------------------EV3341
EV3361
EV3371
EV3381
EJ3341
EJ3361
EJ3371
EJ3381
ES2341-----ES2361
ES2371
ES2381ES3341
ES3361ES3371
ES3381

EB2441
EB2451
EB2461
EB2471
EB2481
EB3441
EB3461
EB3471
EB3481
ER2441
ER2451
ER2461
ER2471
ER2481
ER3441
ER3461
ER3471
ER3481EC2441EC2451
EC2461
EC2471
EC2481
EC3441
EC3461
EC3471
EC3481
EP2441EP2451
EP2461EP2471
EP2481
EP3441
EP3461
EP3471
EP3481
E83441
E83461
E83471
E83481
EF3441
EF3461
EF3471
EF3481
EV3441
EV3461
EV3471
EV3481
EJ3441
EJ3461
EJ3471
EJ3481
ES2441
ES2451
ES2461
ES2471
ES2481
ES3441
ES3461
ES3471
ES3481----------------------------------------EB3781----------------------------------------ER3781----------------------------------------EC3781----------------------------------------EP3781---------------E83781-----------------------------------EV3781---------------EJ3781----------------------------------------ES3781-------------------------EB3841-----EB3871
EB3881-------------------------ER3841-----ER3871
ER3881-------------------------EC3841-----EC3871
EC3881-------------------------EP3841-----EP3871
EP3881---------------E83881-----------------------------------EV3881---------------EJ3881-------------------------ES3841-----ES3871
ES3881

USP category

L1

USP category

L1

L62

L7

L11

L43

L43

L10

L3

18

Sunniest C18, C18-HT
RP-AQUA, C8, PhE, Biphenyl,
PFP, PFP&C18, Cyano, Silica✻Our distributors in the world
Technolab-Sorbent AB
Biotech AB
Inacomdichrom
Instruments
BioAnalytic
Labquip
Watrex Praha, s.r.o. Wuhan Puli Technology Co., Ltd.
Simkon Kft.
BeijingbTai-ke-MeibHi-Tech Co., Ltd.
Obrnuta faza j.d.o.o.
LabService Analytica S.r.l.
BGB Analytik AG
MZ-ANALYSENTECHNIKSalt Analitik Çözümler Kimya
San. ve Tic. Ltd. Şti.

Microcolumn S.r.l.

Yair Technologies Ltd

ChromaTech Ltd
J Raven Pharma Laboratories

Pyvot (Innovations United)
Nacalai USA, Inc.

Espora Lab
Zhejiang Fuli Analytical Instrument Co., Ltd
Scientific Hightek Co., Ltd.
TWT Scientific Ltd.

Alpha Calibraciones de Mexico

Easysci Instruments Company Limited
Eco - Chem Inc.
Applied Chemical and Instrument Co., Ltd
IT Technologies Pte Ltd
Allytech Corporation Ltd.
S&B Analitica
Chromatopak
Analytical

Prochrome India

Manufacturer

ChromaNik Technologies Inc.

2107

6-3-1 Namiyoke, Minato-ku, Osaka, 552-0001 Japan
TEL: +81-6-6581-0885 FAX: +81-6-6581-0890
E-mail: info@chromanik.co.jp
URL: http://chromanik.co.jp