Comparison data of 6 kinds ofcore shell C18 columns
Column name
1. Company P C18, 2.6 mm: Kinetex C18
2. Company T C18, 2.6 mm: Accucore C18
3. Company W C18, 2.7 mm: Cortecs C18
4. Company A C18, 2.7 mm: PoroShell C18 EC
5. Company S C18, 2.7 mm: Ascentis Express C18
6. SunShell C18, 2.6 mm
ChromaNik Technologies Inc.

Comparison of standard samples
Column:Company P C18, 2.6 μm 150 x 4.6 mm (26.1 Mpa, 30,800 plate )Company P C18
264Company T C18, 2.6 μm 150 x 4.6 mm (22.7 Mpa, 31,600 plate)
26.1 MPa
11 3Company W C18, 2.7 μm 150 x 4.6 mm (18.5 Mpa, 23,300 plate)27
Company A C18, 2.7 μm 150 x 4.6 mm (30.6 Mpa, 30,200 plate)
51k6=7.4
Company
TC18
Company S C18, 2.7 μm 150 x 4.6 mm (22.2 Mpa, 31,800 plate)
N6=31,600
6
3422.7 MPa
SunShell C18, 2.6 μm 150 x 4.6 mm (21.8 Mpa, 31,900 plate)
Mobile phase: CH3OH/H2O=75/25
7
1 2 k6=7.75
Company W C18 Flow rate: 1.0 mL/min
4N6=23,300
6318.5 MPa
Temperature: 40 ºCSample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
71 2 k6=9.0
55 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene
Company A C18
N6=30,200
k6=5.4N6=30,800

53026444676

SunShell C18
21.8 MPa 48

Hydrogen bonding Hydrophobicity Steric selectivity

30.6 MPa

52 k6=9.7
1 3 N6=31,800
23
1756(Amylbenzene/Butylbenzene)(Triphenylene/o-Terphenyl)

0.48

1.54

1.20

0.35

1.56

1.50

Company W C18

0.38

1.59

1.32

Company A C18

0.42

1.571.25

Company S C18

0.44

1.60

1.31

SunShell C18

0.39

1.60

1.46

Company P C18

Company S C18
Company T C18
22.2 MPa
7k6=10.4
N6=31,900

10121416
Retention time/min(Caffeine/Phenol)

18202224

Comparison of pyridine
321

Company P C18

Column:Company P C18, 2.6 mm 150 x 4.6 mm
Company T C18, 2.6 mm 150 x 4.6 mm
Company W C18, 2.7 mm 150 x 4.6 mm
Company A C18, 2.7 mm 150 x 4.6 mm
Company S C18, 2.7 mm 150 x 4.6 mm
SunShell C18, 2.6 mm 150 x 4.6 mm
Mobile phase: CH3OH/H2O=30/70
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = Uracil
2 = Pyridine
3 = Phenol

3Company T C18

213Company W C18

2132Company A C18

132

Company S C18

123SunShell C18

1012345Retention time/min

678910111213

Comparison of oxine, metal chelating
compound12

TF=1.24Company P C18
2Company T C18

1 TF=3.94
2

1

TF=1.56

Company W C18
2

1

TF=2.07

Company A C18
21Company S C18

TF=2.04
10122TF=1.21

34Retention time/min

5Column:Company P C18, 2.6 mm 150 x 4.6 mm
Company T C18, 2.6 mm 150 x 4.6 mm
Company W C18, 2.7 mm 150 x 4.6 mm
Company A C18, 2.7 mm 150 x 4.6 mm
Company S C18, 2.7 mm 150 x 4.6 mm
SunShell C18, 2.6 mm 150 x 4.6 mm
Mobile phase: CH3CN/20mM H3PO4=10/90
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = 8-Quinolinol (Oxine)
2 = Caffeine

SunShell C18678910

Comparison of formic acid1

TF=1.47

TF=1.86

Company P C18

TF=1.45

TF=1.46011321Company T C18
32

TF=1.79 1

TF=3.213212Company W C18

Company A C18

332Company S C18

132SunShell C18
23Retention time/min

456Column:
Company P C18, 2.6 mm 150 x 4.6 mm
Company T C18, 2.6 mm 150 x 4.6 mm
Company W C18, 2.7 mm 150 x 4.6 mm
Company A C18, 2.7 mm 150 x 4.6 mm
Company S C18, 2.7 mm 150 x 4.6 mm
SunShell C18, 2.6 mm 150 x 4.6 mm
Mobile phase: CH3CN/0.1% H3PO4=2/98
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@210nm
Sample: 1 = Formic acid
2 = Acetic acid
3 = Propionic AcidSummary of standard samples
Pressurea

Retentionb

SunShell C18

○21.8

10.4

Ascentis Express C18○22.2

PoroShell C18 EC

PlateC

PyridineOxine

Formic acid Point◎31,900

◎

◎◎

14

9.7

◎31,800△△×7×30.6

9.0◎30,002◎△◎

10Cortecs C18◎18.5

7.7×23,300

×○△6Accucore C18

○22.7

7.4

◎31,600××△

6Kinetex C18

△26.1

5.4

◎30,800×◎◎10a. Mobile phase; methanol:water=75:25, 40 oC, 1mL/min, 150 x 4.6mm
b. Retention factor of amylbenzenec. Theoretical plate of amylbenzene◎: 3 point, ○: 2 point, △: 1 point, ×: 0 point

Characteristics
Carbonloading (%)
SunShell C18

Specific surface Pore volume aarea a (m2/g)(mL)

Pore diameter a(nm)7.3 (7)b125 (150)b

0.261

8.34 (9)b8.0

133 (150)b

0.278

8.20 (9)b

PoroShell C18 EC

8.5 (8)b

135 (130)b

0.414

12.3 (12)b

Accucore C18

8.8 (9)b130 (130)b

0.273

8.39 (8)bCortecs C18

7.3 (6.6)b113

0.264

9.32

Kinetex C18

4.9(12 effective)b102(200 effective)b

0.237

9.25 (10)b

Ascentis Express C18

a. Measured after C18 materials were sintered at 600 degree Celsius for 8 hours.
The measured value of each sintered core shell silica is considered to be smaller
than that of the original core shell silica.
b. Value written in each brochure or literature
All data were measured in ChromaNik laboratory.

Particle distribution
12SunSehll C18 (2.54 μm)a
Ascentic Express C18 (2.52 μm)a
Accucore C18 (2.53 μm)a
Kinetex C18 (2.34 μm)aa
PoroShell C18 (2.34 μm)Cortecs C18 (2.77 μm)aNumber/%

108642012Particle diameter/μm

4*Measured using Beckman Coulter Multisizer 3 after C18 materials were sintered
at 600 degree Celsius for 8 hours. The value measure by Coulter Counter method
is smaller than the real value because a porous material includes an electrolyte
solution and the resistance value decreases.
a. Median particle size

8Loading capacity of amitriptyline I
Mobile phase: Acetonitrile/20mM phosphate buffer pH7.0=(60:40)
Column dimension: 150 x 4.6 mm, Flow rate: 1.0 mL/min, Temp.: 40oC

CH3N
CH3160001324

TF=1.18

SunShell C18(core shell)

14000

Theoretical plate

10000
8000
6000
4000

100 times

SunShell C18
Company A C18
Sunniest C18 3um
Company P C18
Company T C18
Compnay W C18
Company S C18

TF=2.43

TF=3.21

T C18(core shell)
W C18(core shell)

TF=3.170.01

Sunniest
C18 3μm(fully porous)
P C18(core shell)

TF=1.25

2000
00.001

A C18(core shell)

TF=1.42

12000

0.1

110Sample weight/mg0

Theoretical plate was calculated by 5σ method
using peak width at 4.4% of peak height.

S C18(core shell)

TF=4.38
1234567Retention time/min

89Sample: 1=Uracil, 2=Propranolol,
3= Nortriptyline, 4=Amitriptyline

10Loading capacity of amitriptyline I
Mobile phase: Acetonitrile/20mM phosphate buffer pH7.0=(60:40)
Column dimension: 150 x 4.6 mm, Flow rate: 1.0 mL/min, Temp.: 40oC
16000
14000

Theoretical plate

12000
10000
8000
6000
4000

100 times

SunShell C18
Company A C18
Sunniest C18 3um
Company P C18
Company T C18
Compnay W C18
Company S C18

2000
00.0010.01

0.1

110Sample weight/mg
Theoretical plate was calculated by 5σ method
using peak width at 4.4% of peak height.

Sample: 1=Uracil, 2=Propranolol,
3= Nortriptyline, 4=Amitriptyline

Loading capacity of amitriptyline IIMobile phase: Acetonitrile/10mM ammonium acetate pH6.8=(40:60)
Column dimension: 150 x 4.6 mm, Flow rate: 1.0 mL/min, Temp.: 40oC
14000

41

Theoretical plate

12000
10000
8000
6000

Sunshell C18
Company A C18
Company P C18
Company T C18
Company W C18
Company S C182330 times

TF=1.20

SunShell C18(core shell)

TF=1.89

A C18(core shell)

T C18(core shell)

TF=2.73

4000

W C18(core shell)

TF=3.12200000.001

P C18(core shell)

TF=2.61

S C18(core shell)

TF=3.240.01

0.1

1Sample weight/mg

10012345678910

Retention time/min

Sample: 1=Uracil, 2=Propranolol,
3= Nortriptyline, 4=Amitriptyline

Loading capacity of amitriptyline III
Mobile phase: Acetonitrile/0.1% formic acid=(30:70)
Column dimension: 150 x 4.6 mm, Flow rate: 1.0 mL/min, Temp.: 40oC
4.5

USP tailing factor

43.5
32.5

Company S C18USP tailing factor

Company T C18

3.6

Company P C18
Company A C18

2.9

Company W C18

2.4

SunShell C18

7 times

1.821.3

1.5
10.00140.01

0.15 min

61Sample weight/μg
In the case of using acetonitrile /0.1% formic acid as a mobile phase, amitriptyline peak shows more tailing because aloading capacity decreases in an acidic, low-ionic-strength mobile phase.

Stability under acidic pH condition

Relative retention/%

100▲

▲

▲

▲

8060

SunShell C18
Company S C18
Company W C18
Company T C18
Company P C18
Company A C18

402000204060Time/h80

100120

Durable test condition
Column size: 50 x 2.1 mm
Mobile phase: CH3CN/1.0% TFA,
pH1=10/90
Flow rate: 0.4 mL/min
Temperature: 80 ºC
Measurement condition
Column size: 50 x 2.1 mm
Mobile phase: CH3CN/H2O=60/40
Flow rate: 0.4 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil
2 = Butylbenzene

Stability under basic pH condition
Relative plate of butylbenzene /%10080
SunShell C1860

Company S C18

Durable test condition
Column size: 50 x 2.1 mm
Mobile phase:
CH3OH/20mM Sodium
borate/10mM NaOH=30/21/49(pH10)
Flow rate: 0.4 mL/min
Temperature: 50 ºC

Company P C18

40Company T C18
Company A C18

20001,000 2,000 3,000 4,000 5,000 6,000

Elution volume/mL

Measurement condition
Column size: 50 x 2.1 mm
Mobile phase: CH3OH/H2O=70/30
Flow rate: 0.4 mL/min
Temperature: 40 ºC
Sample: 1 = Butylbenzene

Summary of stability
Acidic condition Basic condition
pH 1pH 10

pH range written in eachbrochure

SunShell C18◎◎

1.5 - 10

Ascentis Express C18○○

2-9Cortecs C18○not tested

2-8

PoroShell C18 EC△△2-9

Accucore C18△△1 - 11

Kinetex C18△△

1.5 - 10