HPLC column

SunArmor C18
RP-AQUA
New NH2

ChromaNik Technologies Inc.

SunArmor

The evolution of further
surface modification＊

Development of a novel silyl-reagent
which bonded with multifunctional endcapping reagents
Final TMS treatment★C18 phase can be used at pH range from 2
to 12 as well as hybrid C18s.
☆An excellent peak for acidic, basic and metal chelating
compounds without effect of residual silanol groups

Characteristics of SunArnor

1

Stationary Phase

Particle size(µm)

Pore diameter(nm)Specific surfacearea (m2/g)

Carbon loading(%)

LigandpH range forusage

USP Category

SunArmor C18

3 and 512340

17C182 - 12L1

SunArmor RP-AQUA

3 and 512340

18C30

2 - 10L62

SunArmor NH2

3 and 5

12340

6.5

Aminopropyl

2 - 12L8

SunArmor C18 / RP-AQUA / NH2

Stability under basic pH condition
SunArmor C18
Almost same stability to compare with the hybrid C18s.

Durable test condition
Column size: 50 x 2.1 mm
Mobile phase: CH3OH/10mM Ammonium
bicarbonate (pH10.5)=30/70
Flow rate: 0.8 mL/min
Temperature: 60 ºC100

Relative plate/%

SunArmorC18806040Measurement condition
Column size: 50 x 2.1 mm
Mobile phase: CH3CN/H2O=60/40
Flow rate: 0.2 mL/min
Temperature: 40 ºCSample: 1 = Butylbenzene

SunArmor C18
Company A hybrid C18

20Company B Hybrid C18
00200

400

600

800

Elution time/hStability under neutral pH condition at 80 oC
SunArmor C18
Durable test condition
Column size: 50 x 2.1 mm
Mobile phase: CH3OH/10mM
Ammonium acetate (pH6.8)=30/70
Flow rate: 0.2 mL/min
Temperature: 80 ºC

100

Relative plate/%

8060

SunArmor C18

40

Measurement condition
Column size: 50 x 2.1 mm
Mobile phase: CH3CN/H2O=60/40
Flow rate: 0.2 mL/min
Temperature: 40 ºCSample: 1 = Butylbenzene

Company C C18AQ

20002004006008001000

Elution time/h
The result of the comparison data in this catalog is not the representative example of all application.

SunArmor C18 / RP-AQUA / NH22

Stability under basic pH condition
SunArmor
RP-AQUA

SunArmor RP-AQUA

Stability under 100% aqueous basic pH10 condition

Relative plate/%

1008060

SunArmor RP-AQUA

402000100200300400500

Elution time/hDurable test condition
Column size: 50 x 2.1 mm
Mobile phase:
10mM Ammonium bicarbonate (pH10.0)
Flow rate: 0.2 mL/min
Temperature: 40 ºC

3SunArmor C18 / RP-AQUA / NH2

Measurement condition
Column size: 50 x 2.1 mm
Mobile phase: CH3CN/H2O=70/30
Flow rate: 0.2 mL/min
Temperature: 40 ºC
Sample: 1 = Butylbenzene

Parameter using standard sample
SunArmor
C18
RP-AQUA

Evaluation of hydrogen bonding, hydrophobicity
and steric selectivity
Reproducibility in retention

Condition
Column: SunArmor C18, RP-AQUA 5 μm , 150 x 4.6 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºCSample:
1 = Uracil

Separation of standard sample of SunArmor C18, RP-AQUA
12O

3547NH

6N
HOO
H3C

2 = Caffeine

1OOH

2CH3NNNN
CH33 = Phenol
75 64

3

4 = Butylbenzene

CH3

5 = o-Terphenyl

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32
Retention time/min

6 = Amylbenzene

CH3

7 = Triphenylene

Hydrogen bonding(Caffeine/Phenol)

Hydrophobicity(Amylbenzene/Butylbenzene)

Steric selectivity(Triphenylene/o-Terphenyl)

SunArmor C180.40

1.54

1.35

SunArmor RP-AQUA

0.48

1.59

1.43

Reproducibility in retention of SunArmor C183142Batch 101

Batch 102

Batch 103

Retention time of amitriptyline
Batch

Retention time101

7.69 min102

7.97 min1038.12 min104

7.85 min105

7.93 minAverage (Av)

7.91 minStandarddeviation (σ)

0.14 minBatch 104

Batch 10502468

10Condition (amitriptyline)
Column dimension: 150 x 4.6 mm
Mobile phase: Acetonitrile/20mM phosphate buffer
pH7.0=(60:40)
Flow rate: 1.0 mL/min
Temp.: 40oCSample: 1=Uracil
CH3

Retention time/min

O

2=Propranolol

NHCH3OHNH

3= Nortriptyline
4=Amitriptyline

CH3CH3N
CH3The result of the comparison data in this catalog is not the representative example of all application.

SunArmor C18 / RP-AQUA / NH24

Peak Shape of basic compound
SunArmor
C18
Comparison of amitriptyline (4) using ammonium
acetate buffer
1

SunArmor
C18, 5 μm

Comparison of amitriptyline (4) using phosphate buffer
1311
3Company A Hybrid C18, 5 μm

tR=8.43 min
TF＝1.10Sunniest C18, 5 μm

3124323242

14tR=8.82 min
TF＝1.27

Sunniest C18, 5 μmtR=7.69 min
TF＝1.08

SunArmor C18, 5 μm

1tR=8.41 min
TF＝1.103242432tR=3.95 min
TF＝2.03

Company A Hybrid
C18, 5 μm

4 tR=6.23 min
TF＝3.59
4Company B Hybrid
C18, 2.7 μm

tR=5.71 min
TF＝1.45<tR: retention time, TF: tailing factor>

314Company B Hybrid
C18, 2.7 μm

2tR=5.24 min
TF＝1.32<tR: retention time, TF: tailing factor>

Condition (amitriptyline)
Column dimension: 150 x 4.6 mm
Mobile phase:A) Acetonitrile/20mM phosphate buffer pH7.0=(60:40)
B) Acetonitrile/10mM ammonium acetate pH6.8=(40:60)
Flow rate: 1.0 mL/min
Temp.: 40oC
CH3OSample: 1=Uracil
Comparison of pyridine (2) using methanol/water
mobile phase

NHCH3

OH

2=Propranolol
NHCH3

3= Nortriptyline

3

SunArmor C18, 5 μm

4=Amitriptyline

2CH3N
CH3

1

3

Sunniest C18, 5 μm
213Company A Hybrid C18, 5 μm
21

Condition (pyridine)
Column dimension: 150 x 4.6 mm
Mobile phase: CH3OH/H2O=30/70
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = Uracil, 2 = Pyridine, 3 = Phenol

3Company B Hybrid C18, 2.7 μm

O21OHNHNHON

The result of the comparison data in this catalog is not the representative example of all application.

5

SunArmor C18 / RP-AQUA / NH2

SunArmor
C18

Peak shape of acidic and metalchelating compounds

Comparison of oxine (metal chelating compound)
12tR=2.55 min
TF=1.07

SunArmor C18, 5 μmtR=2.77 min
TF=1.07

SunArmor RP-AQUA, 5 μmtR=1.57 min
TF=1.31

Company A Hybrid
C18, 5 μmtR=1.93 min
TF=1.29

Company B Hybrid
C18, 2.7 μm

Comparison of formic acid (acidic compound)21tR=1.96 min
TF=1.49

3SunArmor C18, 5 μmtR=2.02 min 1
TF=1.29

SunArmor
RP-AQUA, 5 μm

Company A Hybrid
C18, 5 μmtR=1.70 min
TF=3.481<tR: retention time, TF: tailing factor>

Company B Hybrid
C18, 2.7 μm

tR=1.65 min
TF=5.24
1Condition (oxine)
Column dimension: 150 x 4.6 mm<tR: retention time, TF: tailing factor>

Condition (formic acid)Column dimension: 150 x 4.6 mm
Mobile phase: CH3CN/0.1% H3PO4=2/98
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@210nm
Sample: 1 = Formic acid
2 = Acetic acid
3 = Propionic Acid

Mobile phase: CH3CN/20mM H3PO4=10/90
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
NSample: 1 = 8-Quinolinol (Oxine)
OHOCH
2 = Caffeine H C
33ONNNN

CH3*Hybrid C18s showed a very poor peak shape forformic acid. It is doubted that some amines as a byproduct remained on the surface of packingmaterials.

The result of the comparison data in this catalog is not the representative example of all application.

SunArmor C18 / RP-AQUA / NH26Stability of SunArmor NH2

Relative retention time/%

SunArmor
NH2SunArmor NH2

100
9080706050
4030
20100Duration test condition
Column: SunArmor NH2 5 μm, 250 x 4.6 mm
Other NH2 5 μm, 250 x 4.6 mm
Mobile phase: Acetonitrile/water = 75/25
Flow rate: 1.0 mL/min,Temperature: 40 oC
Detector: RISample: Sucrose

40 ºC
SunArmor NH2Company A NH2Company B NH20200400600

Relative retention time/%

Elution time/hDuration test condition
Mobile phase: Acetonitrile/water = 75/25
Flow rate: 1.0 mL/min, Temperature: 60 oC

1009080706050
40302010060 ºC
Measurement condition
Mobile phase: Acetonitrile/water = 75/25
Flow rate: 1.0 mL/min, Temperature: 40 oC
Detection: RI, Sample: Sucrose

SunArmor NH20200400600

Elution time/hStability under basic pH
SunArmor NH2

Relative retention time/%

pH11.2 and pH11.8
10090807060
50403020100Duration test condition

Mobile phase:
Acetonitrile/10mM triethylamine pH11.8 = 75/25
Acetonitrile/10mM ammonium bicarbonate pH11.2 = 75/25
Flow rate: 1.0 mL/min, Temperature: 40 oC
10mM ammonium bicarbonate pH11.2
10mM triethylamine pH11.8

0100

200

300

Elution time/h

400500Measurement condition
Mobile phase: Acetonitrile/water = 75/25
Flow rate: 1.0 mL/min, Temperature: 40 oC
Detection: RI, Sample: Sucrose

The result of the comparison data in this catalog is not the representative example of all application.

7SunArmor C18 / RP-AQUA / NH2Separation of sucrose and palatinose

SunArmor
NH212Comparison of retention time
4051015Retention time/minA20

12 3The other stable NH2 showed one third inretention.

1Column: SunArmor NH2, 5 μm 250 x 4.6 mm
Mobile phase: Acetonitrile/50 mM ammonium
acetate=75/25
Flow rate: 1.0 mL/min, Temperature: 40 oC
Detection: RI, Sample: 1 = Sucrose, 2 = Palatinose

3B24051015Retention time/min

20Column: A=Other stable NH2, 5 μm 250 x 4.6 mm
B=SunArmor NH2, 5 μm 250 x 4.6 mmMobile phase: Acetonitrile/Water = 75/25,
Flow rate: 1.0 mL/min, Temperature: 40 oC,
Detection: RI,Sample: 1= Fructose, 2= Glucose, 3= Sucrose,
4= Maltose

Comparison of plate using 100% aqueous samples
1 2
Injection: 0.4 µL

N1 = 17,589
N2 = 19,700

Injection: 5 µL

N1 = 14,796
N2 = 17,522

Injection: 10 µL

N1 = 11,845
N2 = 14,553

Injection: 20 µL

N1 = 6,606
N2 = 9,640

024Retention time/min★Hydrophobic end-capping makes retention short while hydrophilic
end-capping keeps retention. Furthermore, hydrophilic end-capping
makes stability high. SunArmor NH2 shows large retention and highstability.

Separation of branched-chain amino acid
1 26Column: SunArmor NH2, 5 µm 250 x 4.6 mm
Mobile phase: Acetonitrile/10mM ammonium
acetate=70/30
Flow rate: 1.0 mL/min, Temperature: 40 oC
Detection: UV@250nm, Sample: 1 = Uridine,
2 = Cytosine

3024681012Retention time/min

Column: SunArmor NH2, 5 µm 250 x 4.6 mm
Mobile phase: Acetonitrile/10mM ammonium
acetate=70/30
Flow rate: 1.0 mL/min, Temperature: 40 oC
Detection: RI , Sample: 1 = L-Leucine, 2 = L-Isoleucine,
3 = L-Valine

The result of the comparison data in this catalog is not the representative example of all application.

SunArmor C18 / RP-AQUA / NH28

Separation of sugar alcohol

SunArmor
NH2

Separation of cyclodextrin

12314 52300

510510Retention time/min

15Column: SunArmor NH2, 5 μm 250 x 4.6 mm
Mobile phase: Acetonitrile/Water = 80/20
Flow rate: 1.0 mL/min, Temperature: 40 oC
Detection: RI, Sample: 1= Grycerine, 2= Erythritol,
3= Xylitol, 4= Sorbitol, 5= Mannitol

15Retention time/min

Column: SunArmor NH2, 5 μm 250 x 4.6 mm
Mobile phase: Acetonitrile/Water = 60/40
Flow rate: 1.0 mL/min, Temperature: 25 oC
Detection: RI,Sample: 1= α-Cyclodextrin, 2= β-Cyclodextrin,
3=γ-Cyclodextrin

Separation under ion exchange mode and nomarl phase mode
A: 5% Acetic acid in water
B: 5% Acetic acid in acetonitrile

A: 1% Acetic acid in water
B: 1% Acetic acid in acetonitrile

12122121Ion exchange mode

B 0%
B 10%
B 20%
B 30%
B 40%
B 50%
B 60%
B 70%

Normal phase mode
0510Retention time/min

150Column: SunArmor NH2 5μm, 250 x 4.6 mm i.d.
Mobile phase : A) (1% or 5%) Acetic acid in waterB) (1% or 5%) Acetic acid in acetonitrile
Flow rate : 1.0 mL/min, Temperature : 40 oC
Detection : UV@260nm, Injection volume : 1 μL

9SunArmor C18 / RP-AQUA /NH2102030

40Retention time/min

1. Ascorbic acid

2. Erythorbic acid20

SunArmor✻Distributor network

Technolab-Sorbent AB
Biotech ABInacomdichrom
Instruments
BioAnalytic
Labquip
Watrex Praha, s.r.o. Wuhan Puli Technology Co., Ltd.
Simkon Kft.
BeijingbTai-ke-MeibHi-Tech Co.,
Obrnuta faza j.d.o.o.
LabService Analytica S.r.l. Ltd.
BGB Analytik AG
MZ-ANALYSENTECHNIKMicrocolumn S.r.l.

Pyvot (Innovations United)
Nacalai USA, Inc.

Salt Analitik ÇözümlerKimya San. ve Tic. Ltd. Şti.

Espora Lab
Zhejiang Fuli Analytical Instrument Co., Ltd
Scientific Hightek Co., Ltd.
ChromaTech LtdTWT
Scientific Ltd.
Alpha Calibraciones deJ Raven Pharma Laboratories
MexicoEasysci Instruments Company Limited Eco- Chem Inc.
Prochrome India
Applied Chemical and Instrument Co., Ltd
IT Technologies Pte LtdAllytech Corporation Ltd.
S&B Analitica
Chromatopak
AnalyticalYair Technologies Ltd

Ordering information of SunArmor
Innerdiameter(mm)
Length (mm)305075
SunArmor C18, 3 μm
10015025050100
SunArmor C18, 5 μm
150250305075
SunArmor RP-AQUA, 3 μm
10015025050100
SunArmor RP-AQUA , 5 μm
150250150
SunArmor NH2, 3 μm
250150SunArmor NH2, 5 μm
250Packings

2.03.04.610Catalog No. Catalog No. Catalog No.
HB2231----------HR2241
HB2341
HB2441
HB2251---------HB2261
HB2361
HB2461
HB2271
HB2371
HB2471-----HB2381
HB2481
HB3241
HB3341
HB3441
HB3261
HB3361
HB3461
HB3271
HB3371
HB3471
HB3281
HB3381
HB3481
HR2231----------HR2241
HR2341
HR2441
HR2251---------HR2261
HR2361
HR2461
HR2271
HR2371
HR2471-----HR2381
HR2481
HR3241
HR3341
HR3441
HR3261
HR3361
HR3461
HR3271
HR3371
HR3471
HR3281
HR3381
HR3481
HN2271-----HN2471
HN2281-----HN2481
HN3271-----HN3471
HN3281-----HN3481

20

Catalog No.
---------------------------------------------HB3781---------------------------------------------HR3781

USP category

Catalog No.
---------------L1-------------------------L1-----HB3881----------Equivalent to----------L62---------------Equivalent to----------L62
HR3881

L8
HN3781

HN3881

L8

Guard Cartridge column of SunArmor
Particle size

Catalog No.

SunArmor C18, 5 μm Guard cartridge column (1-pak + Holder) 4 x 10mm
SunArmor RP-AQUA, 5 μm Guard cartridge column (1-pak + Holder) 4 x 10mm
SunArmor C18, 5 μm Guard cartridge (4-pak ) 4 x 10mm

5 μm
5 μm
5 μm

HB3A1H
HR2A1H
HB3A1CSunArmor RP-AQUA, 5 μm Guard cartridge (4-pak ) 4 x 10mm
SunArmor Guard cartridge holder

5 μm
---

HR3A1CHHOA1C

product

SunArmor C18 / RP-AQUA / NH210SunArmor

Manufacturer

ChromaNik Technologies Inc.

2103

6-3-1 Namiyoke, Minato-ku, Osaka, 552-0001 Japan
TEL: +81-6-6581-0885 FAX: +81-6-6581-0890
E-mail: info@chromanik.co.jp
URL: http://chromanik.co.jp