REF: P30908

CELL LINES- SARS-CoV-2 (2019-nCoV) Spike HEK293T cell line-

Product Name:

SARS-CoV-2 Spike HEK293T cell line

Catalog Number:

P30908Cell Line:

HEK293

Resistance:

Hygromycin

Format:>3x106 cells in Cryopreserved vials

Storage:

Liquid Nitrogen

About SARS-CoV-2 spike protein

SARS-CoV-2 spike HEK293
The SARS-CoV-2 spike HEK293T cell line

The coronavirus S-protein is the structural

has been developed by stable transfection

protein responsible for the crown-like shapewithof the CoV viral particles.

SARS-CoV-2(2019-nCoV)

spikeprotein expression plasmid. SARS-CoV-2
spike HEK293T cell line provides consistent

The SARS-CoV-2 spike protein mediates

levels of expression of SARS-CoV-2 (2019-the membrane fusion process, and utilizesnCoV) spike protein in cells surface.

human angiotensin-converting enzyme 2(hACE2) as the receptor to infect humancells.
Bibliography: Coutard, B., Valle, C., de Lamballerie, X.,
Canard, B., Seidah, N. G., & Decroly, E. (2020). The spikeglycoprotein of the new coronavirus 2019-nCoV contains afurin-like cleavage site absent in CoV of the same clade.
Antiviralresearch,176,104742.

https://doi.org/10.1016/j.antiviral.2020.104742
Xia, S., Liu, M., Wang, C. et al. Inhibition of SARS-CoV-2(previously 2019-nCoV) infection by a highly potent pancoronavirus fusion inhibitor targeting its spike protein thatharbors a high capacity to mediate membrane fusion. Cell Res
30, 343–355 (2020). https://doi.org/10.1038/s41422-020-0305-

This cell line is intended to be used as anV.A.T. Nº:ESB95481909“in vitro” model for research studies.

INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS, S.L.
Parque Tecnológico Bizkaia, Edf. 502, 1ª Planta | 48160 | Derio | Bizkaia
Tel.: +34 944005355 | Fax: +34 946013455
innoprot@innoprot.com | www.innoprot.com

xRT-PCR analysis

Quality Control

The presence of SARS-CoV-2 spike protein

All cells are performance assayed and test

mRNA was analyzed by RT-PCR.

negative for mycoplasma, bacteria, yeast
and fungi. Cell viability, morphology andproliferative capacity are measured after
recovery from cryopreservation. Innoprot
guarantees stable expression for many
generations and provides support for cell
culture and visualization.

Figure 1. SARS-CoV-2 spike protein RT-PCR
anaylisis. (1) SARS-CoV-2 spike protein HEK293Tcell line. Positive Control (C+): SARS-CoV-2 spike

カタログ番号BBP30908

protein cDNA. Negative Control (C-): not transfected

品

名SARS-CoV-2 Spike HEK293T

HEK293 cells.

容量

3x10^6cells/vial

保存

液体窒素

価格（円・税抜） 1,000,000

Immunofluorescence analysis
The detection of SARS-CoV-2 spike protein

融解用培地：D-MEM(High Glucose)＋10%FBS

in the cells surface was carried out by

培養用培地：D-MEM(High Glucose)＋10%FBS＋1%NEAA＋Hygromycin(80ug/mL)

immunofluorescence analysis with a FITC

操作法につきましては、別資料にてご確認ください。

taggedanti-SARS-CoV-2

spikeprotein

antibody.

本細胞の提供には、MTAが必要になります。

THIS PRODUCT IS FOR RESEARCH PURPOSES ONLY. It
is not to be used for drug or diagnostic purposes, nor is it
intended for human use. Innoprot products may not be resold,Figure 2. Immunofluorescence assay. The

modified for resale, or used to manufacture commercial

image in the left panel shows the membrane

products without written approval of Innovative Technologies

localization of SARS-CoV-2 spike in HEK293T

in Biological Systems, S.L.

cell line. The image in the right panel showsbright field.

株式会社ケー・エー・シー

V.A.T. Nº:
ESB95481909

試薬事業部〒661-0978

INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS, S.L.
Parque Tecnológico Bizkaia, Edf. 502, 1ª Planta | 48160 | Derio | Bizkaia
Tel.: +34 944005355 | Fax: +34 946013455
innoprot@innoprot.com | www.innoprot.com

兵庫県尼崎市久々知西町2丁目1-20（お問い合わせ先）
TEL：06-6435-9747 FAX：06-6435-9748
URL：https://www.saibou.jp/
Email：shiyaku-info@kacnet.co.jp