Regulation of cyclins during the cell cycle
G1/S phase

TGFβ

Replicative senescenceDiﬀerentiationHormones

p57Kip2

Cyclin HP

p18INK4CPP CDK4/6 P

p16INK4A

INK4family

p15INK4B

p53

Cyclin-activating
kinase (CAK)CDK7

p19INK4DUV stress

Replicative senescence

PCyclin D

CDK2- Cyclin A

p21CipCyclin EP- Cyclin E

P

Mitogens

GSK3β

Inhibition
Pof transcription P

MAPKs
c-JunP

c-FosPRb

Cyclin D- CDK1

Cip/Kip family

CDC25A

Mitogen withdrawal

p14ARF

p27Kip1

orDP1- Polymerase a- PCNA

Cyclin EPRb

S phase genes

P

E2F

DP1

E2F

G2/M phase
Beta-TRCP

CDC25A/B/C
P

Dephosphorylation
Wee1

PP

Cyclin B

Myt1P

CDK1

Increased Cyclin B
protein levels

CDK7PP

CDK1 P

Cyclin B

Positive feedback loop
P

CDK1
Cyclin B

Cyclin HP

Wee1Bim

Myt1

PPPPPPP

PLK1

APC/C

Aurora B

CENP-F

P

APC/C
CDC20

PW0304 2024/01 A

Metaphase platealignment

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Spindle assembly
checkpoint extinguished

Cyclin BPP

CDK1UbUb Ub

Proteasomal
degradation

Cyclin B
proteasomal degradation

Anaphaseprogression

Inhibitory
phosphorylation
Activating
phosphorylation

Antibodies
ProductApplication

Species reactivity

AbID

Bim antibody [Y36]

Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, IP, WB

Hu, Ms, Rt

ab32158

Aurora B antibody

Cdk2 antibody [E304]

Cdk4 antibody [EPR4513-32-7]

CDKN2A/p16INK4a antibody [EPR1473]
c-Fos antibody [EPR883(2)]c-Jun antibody [E254]

Cyclin A2 antibody [E142]

Cyclin B1 antibody [Y106]

Cyclin D1 antibody [EPR2241]
Cyclin E1 antibody [EP435E]
E2F1 antibody [EPR3818(3)]GSK3 beta antibody [Y174]
p21 antibody [EPR3993]

p27 KIP 1 antibody [Y236]
p53 antibody [DO-1]

p57 Kip2 antibody [EP2515Y]
Rb antibody [EPR17512]

Inhibitors and activators

ICC/IF, IHC-Fr, IHC-P, IP, WB

Flow Cyt, ICC/IF, IHC-P, IP, WB
Flow Cyt, ICC/IF, IHC-P, WB

Flow Cyt, ICC/IF, IHC-P, IP, WB
Flow Cyt, WB

ICC/IF, IHC-P, IP, WB
ICC/IF, IHC-P, WB

Flow Cyt, ICC, IHC-P, IP, WB
ICC/IF, IHC-P, IP, WB

Flow Cyt, ICC/IF, IP, WB

Flow Cyt, ICC/IF, IHC-P, IP, WB
Flow Cyt, ICC/IF, IHC-P, WB
WB

Flow Cyt, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, IP, WB

ChIP, ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
ICC/IF, IHC-P, IP, WB

Flow Cyt, ICC/IF, IHC-P, IP, WB

Hm, Hu, Ms, Pig, Rt
Hu, Ms, Rt
Hu
Hu

Hu, Ms, Rt
Hu, Ms, Rt
Hu, Ms, Rt
Hu

Hu, Ms, Rt
Hu

Hu, Ms, Rt
Hu, MsAGMk, Hu, Ms, Rt
Hu, RtHu

Hu, Ms, Rt

AGMk, Hu, Ms

ab2254ab32147

ab108357
ab108349
ab134122
ab32137

ab181591
ab32053

ab134175
ab33911ab179445
ab32391

ab109199
ab32034
ab1101

ab75974

ab181616

TargetCompoundDescription

AbID

CDK4/6

Palbociclib

Potent, highly specific inhibitor. IC50 of 11 nM (CDK4) and 16 nM (CDK6).

ab218118

CDK1

GSK3βp21
p53
Rb

Other related products

Ro 3306
BIO

UC2288RITA

Silibinin

Selective inhibitor. Ki of 35 and 110 nM for CDK1/Cyclin B1 and CDK1/Cyclin A.
Cell-permeable, selective, reversible inhibitor. IC50 of 5 nM (GSK3α/β).
p53-independent, selective p21 attenuator. GI50 of 10 μM.

Induces p53 activation in tumor cells. Prevents p53-HDM2 (MDM2) interaction.

Increases unphosphorylated Rb (active) 4-fold and increases Rb-E2F complexes.

ab141491
ab120891
ab146969
ab219379
ab142948

ProductDetails and applications

Species reactivity

AbID

CDKN2A/p16INK4a protein

SDS-PAGE-ab84075

CDK1 ELISA Kit

GSK3 beta (pS9 + Total) ELISA Kit
GSK3 beta protein

GSK3 beta pS9 ELISA Kit
GSK3 beta ELISA Kit
p21 ELISA Kit
p21 ELISA Kit
p21 protein

p53 ELISA Kit
p53 protein
Rb protein

abcam.com

Copyright © 2024 abcam. All rights reserved.

90-min ELISA kit made with recombinant monoclonal antibodies
90-min ELISA

Functional studies, SDS-PAGE, western blot
90-min ELISA
90-min ELISA

90-min ELISA kit made with recombinant monoclonal antibodies
90-min ELISA kit made with recombinant monoclonal antibodies
Functional studies, SDS-PAGE, western blot
90-min ELISA

Functional studies, SDS-PAGE, western
Functional studies, SDS-PAGE

Hu

Hu, Ms, Rt (predicted)-

Hu, Ms, Rt (predicted)
Hu, Ms, Rt (predicted)
Hu

Ms
-Hu
-

ab212162
ab205711
ab60863ab205709
ab205710
ab214658
ab205576
ab56278

ab171571
ab43615
ab56270