筑波大学技術報告24: 1-4, 2004‫ڥ‬Ϝଳ২ƳƒƙǓǦȦǲǡțƶઓ໔ơǓమƬƶ๡ԥ࢖ߤҥન1¹অ௞ஞў2૿ౡપԙॶյ੒‫ݜ‬௖ؐА‫ࠊףڽ‬ѬࡋáЌԙ‫ڈ‬âĞ305-8575 Иश‫ۀ‬ƬƗƸࠅஔ҉઩ 1-1-1Ӷ຤৯Ƴԏ໔ƟƧȜǦȆǵțưൟ৖ָࢾǚພƌƮࡑ‫ۊ‬
Ɵ¹‫ڥ‬Ϝଳ২ƳƒƌƮ¹ǦȦǲǡțƷઓ໔ơǓమƬƶ߁ພǚ࠮ƬƛưǚฯǑƓƳƟƧºГƬƷ¹‫ܢ‬Ɨ૯ǑǔƮƌǓงऊƶ‫ڥ‬ը൲ԫ‫ص‬ǚӓƟƧअϜ߁ພƯƊǒ¹మƬ๋Ʒ¹ଇঞƶǦȦǲǡțϴ੻঴ȄÓȌȚȮ‫ݜ‬঵‫ڈ‬ǚӓƟƧ‫ݔ‬Ϝ߁ພƯƊǓº‫ࡣۼ‬Ʒ¹షऺƶ‫ݗ‬ǦȦǲǡț॒Ƴǐǒ‫ڥݗ‬ϜऔǚຊේơǓरƯ‫ئ‬ǉƮࢲພƲǊƶƯƊǓºŌ¼ƷƠǉƳǦȦǲǡțư‫ڥ‬Ϝƶշ‫ٱ‬Ʒ¹ൟࠃি൝ԙƓǑ༂ࣽ໹ЏǆƯ‫ܢ‬Ɨ‫ףڽ‬ƝǔƮƕƧºંƗƶ‫ࡣףڽ‬Ʒ¹‫ڥ‬ଇǦȦǲǡțƶ੢ҦƔ‫ڥ‬ը൲ԫ‫ص‬ǚࢌࢹƟ¹෱ߕǚ‫ݗ‬ǉǓࠧƳǐǒ‫ڥ‬ϜƔरअơǓࠧǚඦ‫ݥ‬ƟƮƌǓºƥƶƧǉ¹ƊǓࡾƶǦȦǲǡțȑȨǾǦÓ‫ޱ‬Ɣ‫ڥݗ‬Ϝऔƶ࠲໰๣ưƟƮ߹ພƝǔƮƌǓºГධ¹ћԙଳ‫ލ‬ǌ௫൝ࡑ‫ۊ‬ƳǐƫƮ¹ǦȦǲǡțƔ‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓࠧƔ࠷‫މ‬ƝǔƮƒǒ¹‫ڥ‬ϜƳઓơǓǦȦǲǡțƶ੏ಶơǓ߁ພƷ¹ЌԙरƶપƕƲȁÓșƶГƬƳƲƫƮƌƧºअϜ߁ພƳƬƌƮƷ֏ਨஇȜǦȆǵțƔӕฯƝǔƮƌǓƔ¹‫ݔ‬Ϝ߁ພƳշƟƮƷӕฯƝǔƮƌƲƌº¸৯ƳӬದƟƧ Brain Mapping Analyzer ǌ݉௫ԙஇ¹
๣໊ԙஇ¹িҠԙஇࡹ඲ǚພƌƮ¹షऺƶিԩǚୋƠƮ৤ࡷƝǔƧǦȦǲǡțƔ¹ଇঞƶǦȦȝǳȠȥ
Ȯǚԩ঴ҠƟ¹ǺǟȨǲȮ঎߱Ҡ‫ݏ‬ਬáǦȁǮȦǝȚȮ‫ݜ‬঵ƶ໒ੱ‫ݏ‬ਬâǚിԩҠƟ¹Сƕ੶ƕȄÓȌȚȮáǦȁǮȦǝȚȮƶГƬƯ¹३‫ډ‬ஞૄ൝ࡐâƶ‫ݜ‬঵ౖǚ‫ݗ‬ǉǓࠧǚӕฯƟƧ [1-4]ºƛƶȜǦȆǵțǚບÐƲࡏഗƶದऔ֞ࣥӕฯƳ҃ພƟƮƕƧƔ¹ƥƶГգưƟƮǦȦǲǡțƶ‫ݔ‬Ϝ߁ພǚ‫ףڽ‬ƟƮƕƧºō¼ǦȦǲǡțƳǐǓ౗ఝȄÓȌȚȮƶ¸¸‫ݜ‬঵ଳ২ƷƠǉƳ¹ǦȦǲǡțƳǐǓ౗ఝȄÓȌȚȮƶ‫ݜ‬঵ଳ২ƶȜǦȆǵțƳշƟƮթ૒ƳࢿǁǓºșǡǴƶൕ݅ఝƳǣǺȉÓȦǚேຌơǓư¹݉௫ƶरƯঐมीઙƳոǓºƛƶǣǺȉÓȦ຃ದ঴ƶঐม࠯յƶସƝƷ¹౗ఝিઑǝȚȮǦȁǮȦǝȚȮƶઁ¹ǶȨȃȆȮ௖Ǌսǈƶ౔ஶƳǐƫƮଳ২ƝǔƮƌǓƔ¹ǦȦǲǡțƶ౗ࡋఝேຌƳǐƫƮǊ౔ஶϴ੻இƳ੢ҦơǓºƛƶঐม࠯յǚѩସơǓǦȦǲǡțƶܑүƷ¹ǦȁǮȦǝȚȮƶ‫ݜ‬঵‫ݏ‬ਬਰӳ‫ޱ‬ǌǦȦȝǳȠȥȮዓ‫ޱܨ‬ƳǐƫƮຫƐǑǔǓºƛƶ‫ڤ‬үƓǑ¹ǦȦǲǡțƔǦȦȝǳȠȥȮ‫ڈ‬ǚӓƟƮǦȁǮȦǝ12ȚȮƶ‫ݜ‬঵‫ݏ‬ਬǚിԩҠƟ¹౔ஶƶ‫ݗ‬ƗƲƫƧǦȁǮȦǝȚȮƔঐม࠯յǚѩସƝƣƧư࠷‫މ‬ƝǔǓ[2] ºƛƶࡑ‫ۊ‬ǚ֏Ƴ¹‫ੱݗ‬њઑǪȨșȃǫȤȐǞÓƳǐ
ƫƮ¹౗ఝƶǦȁǮȦǝȚȮ౔ஶƳઓơǓǦȦǲǡțƶܑүǚൟ৖ƟƧºƥƶ‫ڤ‬ү¹ǦȦǲǡțƶேຌƳǐƫƮપ౗ਙઑƯ๢ '*ĊƶȄÓȌȚȮƶ੢ҦƔԏీƝǔƧ [1] ºƟƓƟ¹িҠԙஇධ඲ƯƷ¹ȄÓȌȚȮƶ‫ݜ‬঵ƔƱƶ൉ϳƯ‫ݗ‬ǉǑǔƧƶƓधެƲ௬୩ƔƯƕƲƓƫƧºƛ ƶ ൉ ϳ ǚ ฯ Ǒ Ɠ Ƴ ơ Ǔ Ƨ ǉ ¹ Brain Mapping
AnalyzerǚӬದƟƧ [5] ºƛƶਗ਼૷Ʒ¹ົ࡜‫ۉ܎ڏ‬೰‫؛‬ƶǦȜȤșǡȮȃƳ‫ݗ‬Օஶƶ܎஢ࠃ੢౵ըǚǶǾȃƟ¹܎༲ƳȏȮȖÓȦǚ੃హƟƮƌǓƧǉ¹഍෭ఝƶ೰ࣹƲГ஝ƓǑದƣǑǔǓ‫܎ڏ‬ƨƙǚਏવƟƮ੯܎ƯƕǓºƝǑƳ¹‫ۉ‬೰‫؛‬ƶ஢௫ǴȁÓǳƔǮȮȏȠÓǺÓƯর‫۽‬ƝǔƮƒǒ¹మ࠰‫ی‬ධ‫ܕ‬Ƴ %#'* µbϱरƶǴȁǾȒ൒ƯЃ௫ơǓƧǉ¹഍෭ਙ฻ǚ೰ެƳǴǨȞȮơǓƛưƔƯƕǓºГஶƳࣹ௫൝ƶ౗ৡඅਙઑǚൟ৖ơǓƛưƔҧౖƯ¹ԇ໹Џƶ‫܎ڏ‬؊ஶƶൟരǚެභȧȔȦƯ୩໷ƯƕǓºȁȧȎǦȜȤǚພƌƧӋ੡ӕ৖඲ǌ஢֡Ҡԙ‫֎ࢽڸ‬ǚພƌƧ‫ੱݗ‬њઑǪȨșȃǫȤȐǞÓ඲ưೝǁƮ¹ࣺƲƗưǊ)‫ڞ‬ϱर೰ެƯ়ԏƲൟ৖‫ڤ‬үƔ௻ǑǔǓºƟƓǊ¹౗ৡඅरƶ೪௾ЂஇƲ‫܎ڏ‬ǚՏਙƳऎ‫׫‬ơǓƛưǊҧౖƯ¹࣐঑Ʋ३‫ډ‬Ҡԙ൝ࡐƨƙǚਏવƟƮ୩໷ƯƕǓઁ¹Г෸ƶ഍෭ƓǑൖঝƶ൝ࡐǚۛൿƳ୩໷ƟƮ¹ƥǔǑƶൟരǚೝԔӕ৖ơǓƛưǊҧౖƯƊǓ[4,5]º¸Г෸ƶȤǾȃƶ౗ৡඅǚǺǟȨǲȮ঎߱Ҡ‫ݏ‬ਬ¹ǦȦȝǳȠȥȮϴ੻঴ȒȨȁǞȮǨȅÓǷ>> ‫ח‬ƻǦȦȝǳȠȥȮƶ‫ܨ‬ઑƳǐƫƮ‫ื܎ڏ‬ћਭॎҠԙஇƳߡࢲ৾ॐƟ¹Brain Mapping Analyzer ǚພƌƮ '*ญ໹ЏƳൟԥƟ¹ƥǔƦǔƶ൝ࡐƶൟരǚ୩໷இƳൟ৖ƟƧºਅऽઑư੩‫ޔ‬ԌƳƷ¹ߡ൝ࡐƔ‫؁‬Ƴ‫౔ݗ‬ஶƯൟരƟƮƒǒ [4] ¹ƝǑƳ¹ƛǔǑƶ൉ϳƯǦȦǲǡțƔǦȦȝǳȠȥȮϴ੻‫ڈ‬ǚӓƟƮȄÓȌȚȮƶ‫ݜ‬঵‫ڈ‬ǚଳ২ƟƮƌǓࠧǚԏీƟƧ[3]ºŎ¼ଇঞǦȦǲǡțϴ੻঴ƶ‫ݔ‬Ϝ߁ພଇঞȄÓȌȚȮƶ੢ҦƷ¹‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓࠧƔ૯ǑǔƮƌǓºǦȦǲǡțƔȄÓȌȚȮƶ‫ݜ‬঵ǚ‫ݗ‬ǉǓࠧǐǒ¹ȧȔȦƶ‫ݗ‬ƗƲƫƧȄÓȌȚȮƔ‫ڥ‬ϜǚҟƚǓࠧƔ঍ߒƝǔǓƶƯԏీƟƧº¸ԑ৊ȤǾȃƶ੩౗ࡋƳǦȦǲǡțǚேຌơǓư¹ேຌ‫ۼ‬őൟƓǑŌ࠯յϱरƳ஬ǒ‫ڥ‬ϜƔ๺ϼƳ‫ݔ‬ҟơǓºƛƶǦȦǲǡțƶ‫ݔ‬Ϝ߁ພƷ¹ǦȦȝǳȠȥȮƶ௾ЂஇƲዓ‫ޱܨ‬¹ǦȁǮȦǝȚȮƶ‫ݜ‬঵‫ݏ‬ਬਰӳ‫ޱ‬¹३‫ډ‬২ࡦ૨๣ϦƌƷȄÓȌȚȮ D2 ȧǶȒǺÓਰӳ‫ޱ‬ƶҢǔƓƶேຌƳǐƫƮຫরƝǔǓƔ¹Ȅ

E-mail: akiyamak@md.tsukuba.ac.jp; Tel: 029-853-3330
E-mail: dsutoo@md.tsukuba.ac.jp; Tel: 029-853-3113-1-ÓȌȚȮ D1 ȧǶȒǺÓਰӳ‫ޱ‬ƶேຌƳǐƫƮƷҢƶ඄ҠǊ‫ۂ‬ǑǔƲƌ[6-8]áইŌâº¸ƛƶ‫ڤ‬үǐǒ¹ଇঞƶǦȦǲǡțƷ¹ǦȦȝǳȠȥȮϴ੻‫ڈ‬ǚӓƟƮȄÓȌȚȮƶ‫ݜ‬঵ǚ‫ݗ‬ǉ¹ȧȔȦƶ‫ݗ‬ƗƲƫƧȄÓȌȚȮƔ D2 ȧǶȒǺÓǚӓƟƮ‫ڥ‬Ϝଳ২ଇঞƳ߁ພƟ¹‫܉‬Օ३‫ڈډ‬ǚຫরơǓࠧƳǐƫƮ‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓư݂ƐǑǔǓºŏ¼‫ڥ‬ଇƶǦȦǲǡțư‫ڥ‬Ϝ¸౗ࡋఝƳேຌƟƧǦȦǲǡțƔȄÓȌȚȮƶ߁ພǚӓƟƮ‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓࠧǚԏీƟƧƔ¹งऊƓǑேຌƟƧǦȦǲǡțƳǐƫƮǊ௬ƠܑүƔ௻ǑǔǓƓǚԏీƟƧºǦȦǲǡțǚৌฝఝƳேຌơǓư¹‫ڥ‬ը൲ԫ‫ص‬ǚࢌࢹƟ‫ڥ‬ϜƔरअơǓƧǉ¹ଇঞƯƶ‫ݔ‬Ϝಶ҃ǚԏీơǓƛưƔƯƕƲƌºƥƛƯ¹ǦȦǲǡțȑȨǾǦÓ‫ޱ‬ƳǐƫƮ¹‫ڥ‬ը൲ԫ‫ص‬ƶࢌࢹǚຫরƟƲƔǑଇঞƶ‫ݔ‬Ϝಶ҃ǚհߒƟƧºȤǾȃƶৌฝఝƳǦȦǲǡțȑȨǾǦÓ‫ޱ‬ǚேຌơǓư¹Г࠯இƲ‫ڥ‬Ϝƶ‫ݔ‬ҟƔ‫ۂ‬ǑǔǓºƛƶܑүƷ¹ǦȦǲǡțƶৌฝఝਕேຌƳǐƫƮ੢ҦƟ¹ǦȦǲǡțƶܑүƷ¹౗ࡋఝƳਕேຌƟƧǦȦǲǡțǨȧÓȃ‫ޱ‬ƳǐƫƮຫরƝǔǓáইōâºƛƶ‫ڤ‬үǐǒ¹‫ڥ‬ଇƶǦȦǲǡțƶГ൉Ʒଽৣ‫ڥ‬ըƳ߁ພƟ‫ڥ‬Ϝǚ‫ݗ‬ǉǓƔ¹Г൉Ʒ౗ఝƳЃ݉Ɵ¹ȄÓȌȚȮƶ߁ພǚӓƟƮ‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓࠧƔ࠷‫މ‬ƝǔǓ[9] ºਕࡣƷ‫כ‬঴ಶ҃ƯƊǒ¹‫ࡣۼ‬Ʒฎ঴இƲಶ҃ƯƊǓºইō¼ԑ৊ȤǾȃƶ൲‫ج‬௫ฝϜƳઓơǓǦȦǲǡțȑȨǾǦÓ‫ޱ‬ƶৌฝఝேຌƶܑүইŌ¼ԑ৊ȤǾȃƶ൲‫ج‬௫ฝϜƳઓơǓǦȦǲǡțƶ౗ࡋఝேຌƶܑү

र¾ǦȦǲǡțȑȨǾǦÓ‫( ޱ‬Verapamil, 300 µg/kg) ƶৌฝఝேຌƳǐƫƮ¹๺ϼƲ‫ݔ‬Ϝಶ҃ƔǇǑǔǓºଇ¾Г࠯յਕƳѻҠǦȦǲǡț (300 µmol/kg) ǚৌฝఝேຌƟƧȤǾȃƯƷ¹ǦȦǲǡțȑȨǾǦÓ‫ޱ‬ƳǐǓ‫ݔ‬Ϝಶ҃Ɣ¹๢ŏ౵࠮੶ơǓºҟ¾ŐൟਕƳǦȦǲǡțǨȧÓȃ‫ޱ‬ǚ౗ࡋఝƳேຌơǓư¹ǦȦǲǡțƶৌฝఝேຌƶܑүƷವ‫ۑ‬ơǓº¸ѻҠǦȦǲǡț (30 µmol/kg) ƶ੩౗ࡋǀƶேຌƳǐ
ƫƮ¹Гӄ঴ƶअϜಶ҃ưŌ࠯յϱरƳ஬Ǔ࠮੶ƟƧ๺ϼƲ‫ݔ‬Ϝಶ҃ƔǇǑǔǓáरâºƛƶǦȦǲǡțƶܑүƷ¹ǦȦȝǳȠȥȮዓ‫ޱܨ‬ƶ௬࠯ேຌáଇâǌ¹ǺǟȨǲȮ঎߱Ҡ‫ݏ‬ਬਰӳ‫ޱ‬ƶਕேຌáҟâƳǐƫƮऎࡉơǓºĢ8 ǦȦǲǡțȑȨǾǦÓ‫ޱ‬ேຌ஝¹*p<0.05; **p<0.01;***p<0.001¹ Ǧ Ȧ ǲ ǡ ț ȑ Ȩ Ǿ Ǧ Ó ‫ ޱ‬ே ຌ ਕ ư ೝ Ԕ(Student’s t-test)º(Sutoo et al., 1988) Y7[¸ġ8 ǦȦǲǡțேຌ஝¹*p<0.05; **p<0.01¹ǦȦǲǡțேຌਕưೝԔ (Student’s t-test)º(Sutoo et al., 1987) [6]-2-¸ইŎ. SHR ƶ ‫ݱ‬ǦȦǲǡțൟര
13 ࢢ ᲊ ƶ ࠺ਘ ದ औ ‫ ڥ ݗ‬Ϝ Ȥ Ǿ ȃ(SHR) ư ඟ ‫ ڈ‬ƶ
WKY ǚ X ਅߔыƟ¹௻ǑǔƧȐǞȦțǚ Brain
Mapping Analyzer ƳǐƫƮ୩໷இƳӕ৖ƟƧºSHR
Ʒ¹WKY ưೝԔƟƮਙॱƳ஬ƫƮ‫ݱ‬ǦȦǲǡțȧȔȦƔ‫ݗ‬ƌº(Sutoo et al., 1993) [10]Ő¼‫ڥݗ‬ϜऔȝȂȦ௫൝ƶӕ৖‫ڥݗ‬ϜऔƶȝȂȦ௫൝ưƟƮ‫ܢ‬Ɨ૯ǑǔƮƌǓ࠺ਘದऔ‫ڥݗ‬ϜȤǾȃ(SHR)ƶǦȦǲǡțϴ੻঴ȄÓȌȚȮ‫ݜ‬঵‫ڈ‬ƳշຌơǓࡾÐƶ൝ࡐǚൟ৖ƟƧºඟ‫ڈ‬ƶ‫ऺګ‬ȤǾȃ(WKY)Ƴೝǁ¹SHR Ʒ‫ݱ‬ǦȦǲǡțȧȔȦƔ๺ϼƳ‫ݗ‬ƗáইŎâ¹‫ג‬Ƴ‫ڥ‬ঽǦȦǲǡțȧȔȦƔୢƌºƛƶࠧƔ¹ଇঞǀƶǦȦǲǡțƶ‫ר׼‬ǚ‫ࣺۑ‬Ɲƣ¹౗ఝƶǦȦȝǳȠȥȮϴ੻঴ȄÓȌȚȮƶ‫ݜ‬঵ǚୢҟƝƣƮƌǓư࠷‫މ‬ƝǔǓºࡑް¹SHR ƶਅऽઑư੩‫ޔ‬ԌƶȄÓȌȚȮȧȔȦƷ¹WKY Ƴೝǁ๺ϼƳୢƗáইŏâ¹ǦȦǲǡțƶ౗ࡋఝேຌƳǐƫƮӡਖƝǔǓºƛǔƔ¹SHR ƳǇǑǔǓ‫ڥݗ‬Ϝऔƶದऔ‫ۍ‬ПƶГƬư݂ƐǑǔǓ[10,11]ºইŏ. SHR ƶ ౗ఝȄÓȌȚȮൟര
13 ࢢ ᲊ ƶ ࠺ਘ ದ औ ‫ ڥ ݗ‬Ϝ Ȥ Ǿ ȃ(SHR) ư ඟ ‫ ڈ‬ƶWKY ǚᓕ໙۞୩Ɵ¹౗ৡඅǚȄÓȌȚȮƳઓơǓ‫ܨ‬ઑƳǐƫƮืћਭॎҠԙஇƳ৾ॐƟƧºԇৡඅǚ Brain Mapping Analyzer ƳǐƫƮ୩໷இƳӕ৖ƟƧºSHR ƶਅऽઑư੩‫ޔ‬ԌƯ๺ϼƲȄÓȌȚȮƶ‫ࣺۑ‬ƔԏీƝǔǓº(Sutoo et al., 1993) [10]ഒŌ¼ȤǾȃƶࢌࢹ֛‫ڥ‬ϜƳઓơǓǦȦǲǡțƶ‫ܔډ‬৤ࡷƶܑүТ໬঎ưƟƮ 1.5%ѻҠǦȦǲǡțຜњǚ৤ࡷƝƣǓư¹Ŕష๋ƓǑ๺ϼƳ‫ڥ‬ϜƔ‫ݔ‬ҟơǓºǦȦǲǡțƶܑүƷ¹ǺǟȨǲȮ঎߱Ҡ‫ݏ‬ਬਰӳ‫ޱ‬αMPTƶ౗ࡋఝேຌƳǐƫƮຫরƝǔǓºԇ‫ڥ‬Ϝ૮Ʒ¹ேຌਕƶ‫ڥ‬Ϝǚ‫ތ‬ƟСƌƧ૮ǚ࠷ƟƮƌǓºa p<0.05; b p<0.01¹ water + ি໊॒ѻ঎ saline ேຌ٫ưೝԔ¿c p<0.05; d p<0.01¹
CaCl2+ saline ேຌ٫ưೝԔ Newman-Keuls t-testºSutoo et al., 1990 [12]ő¼‫ݗ‬ǦȦǲǡț॒ƳǐǓ‫ڥ‬Ϝଳ২ȤǾȃƳ¹ฎ঴இƳ‫ݗ‬ǦȦǲǡțТ໬঎ǚຌƐǓư¹Ŕష๋ƓǑ๺ϼƳ‫ڥ‬ϜƔ‫ݔ‬ҟơǓºƛƶǦȦǲǡțƶܑүƷ¹ǦȁǮȦǝȚȮƶ‫ݜ‬঵‫ݏ‬ਬਰӳ‫ޱ‬ƶ౗ࡋఝேຌƳǐƫƮຫরƝǔǓáഒŌâºƝǑƳ¹ǦȦǲǡțƶൕ݅ఝேຌƳǐƫƮǊ௬ບƲ‫ݔ‬Ϝಶ҃ƔǇǑǔǓºƛƶ‫ڤ‬үǐǒ¹‫ܔډ‬ϦƌƷൕ݅ఝƓǑฎ঴இƳேຌƟƧǦȦǲǡțǊ¹౗ࡋఝ‫ח‬ƻৌฝఝƳேຌƟƧष‫ݜ‬ư௬ບƳଇঞȄÓȌȚȮƶ‫ݜ‬঵ǚ‫ݗ‬ǉ¹ࣦÐƳ‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓƛưƔ࠷‫މ‬ƝǔǓ[12]º-3-‫ؼޚ‬¹ԇ‫ݦ‬Ư‫ݗ‬ǦȦǲǡț॒ƳǐƫƮ‫ڥݗ‬Ϝऔǚຊේ½࠲໰ơǓࠟǇƔࠀǆƫƮƌǓ [7]º‫ړ‬ஶƶ‫ڥݗ‬ϜՔࡣƳ‫ݗ‬ǦȦǲǡțǚս๺ơǓޭ॒ǚຌƐǓư‫ڥ‬ϜƔ‫ݔ‬ҟƟ¹ఴ॒ǚ‫ޙ‬ӬơǓư‫ޙ‬ƻ‫ڥ‬ϜƔरअơǓƛưƔඦ‫ݥ‬ƝǔƮƌǓºƝǑƳ¹‫ת‬సƶ৤ࡷ໷ư‫ڥ‬ϜƳƷാƶ੏շƔƊǒ¹෹ష‫ת‬సǚ৤ࡷơǓư‫ڥ‬ϜƔ‫ݔ‬ҟơǓƛưƔඦ‫ݥ‬ƝǔƮƌǓºŒ¼ǆưǉ‫ڥ‬Ϝଳ২ƳƒƌƮ¹ǦȦǲǡțƷઓ໔ơǓమƬƶ߁ພǚ࠮ƬºГƬƷ¹งऊƶ‫ڥ‬ը൲ԫ‫ص‬ǚӓƟƧअϜ߁ພƯƊǒ¹ǊƎГƬƷ¹ଇঞƶǦȦǲǡțϴ੻঴ȄÓȌȚȮ‫ݜ‬঵‫ڈ‬ǚӓƟƧ‫ݔ‬Ϝ߁ພƯƊǓºƛƶ‫ݔ‬Ϝ߁ພƶȜǦȆǵțƷϱҟƶǐƎƳ݂ƐǑǔǓº॒ࠧƲƱƳǐƫƮ‫ڥ‬ଇǦȦǲǡțƔࣺƟƢƬ੢ҦƟƮǊ¹‫ڥ‬ըǚߺ‫ڜ‬ơǓ౔ஶƯƷƲƌƶƯࢌࢹƝƣƲƌº੢ҦƟƧ‫ڥ‬ଇǦȦǲǡțƶГ൉Ʒ౗ఝǀЃ݉Ɵ¹ǦȦȝǳȠȥȮϴ੻‫ڈ‬ǚӓƟƮȄÓȌȚȮƶ‫ݜ‬঵ǚིॳơǓºȧȔȦƶ‫ݗ‬ƗƲƫƧȄÓȌȚȮƔ D2 ȧǶȒǺÓǚӓƟƮ‫ڥ‬Ϝଳ২ଇঞƳ߁ພƟ¹งऊƶ‫܉‬Օ३‫ڈډ‬ǚຫরƟƮ‫ڥ‬Ϝǚ‫ݔ‬ҟƝƣǓáইŐâ[7]ºইŐ¼ǦȦǲǡțƳǐǓ‫ڥ‬Ϝଳ২ƶȜǦȆǵț (Sutoo et al., 1997) [7]ߣ݂൫‫ڼ‬[1] D. Sutoo, K. Sano, Modulating effects of biogenic
amines on calcium and ethanol-induced sleeping time,Alcohol 1 (1984) 141-144.
[2] D. Sutoo, K. Akiyama, K. Iimura, Effect ofcalmodulin antagonists on calcium and ethanolinduced sleeping time in mice, Pharmacol. Biochem.
Behav. 23 (1985) 627-631.
[3] D. Sutoo, K. Akiyama, M. Geffard, Central
dopamine-synthesis regulation by the calciumcalmodulin-dependent system, Brain Res. Bull. 22(1989) 565-569.
[4] D. Sutoo, K. Akiyama, K. Yabe, Comparison
analysis of distributions of tyrosine hydroxylase,calmodulin
andcalcium/calmodulin-dependent
protein kinase II in a triple stained slice of rat brain,
Brain Res. 933 (2002) 1-11.
[5] D. Sutoo, K. Akiyama, K. Yabe, Quantitativemapping analyzer for determining the distribution ofneurochemicals in the human brain, J. Neurosci.
Methods 85 (1998) 161-173.
[6] D. Sutoo, T. Matsukura, K. Akiyama, Effect of intraventricular administration of calcium and calmodulin
antagonist on the blood pressure in the rat, Neurosci.
Lett. 82 (1987) 297-302.

[7] D. Sutoo, K. Akiyama, Regulation of blood pressure
with calcium-dependent dopamine synthesizing
system in the brain and its related phenomena, Brain
Res. Rev. 25 (1997) 1-26.
[8] D. Sutoo, K. Akiyama, Effect of dopamine receptor
anatagonists on the calcium-dependent central
function that reduces blood pressure in spontaneously
hypertensive rats, Neurosci. Lett. 269 (1999) 133136.
[9] D. Sutoo, T. Matsukura, K. Akiyama, The mechanism by which calcium reduces blood pressure, Eur.
J. Pharmacol. 155 (1988) 189-192.
[10] D. Sutoo, K. Akiyama, T. Matsukura, R. K.
Nakamoto, Decrease of central dopamine level in theadult spontaneously hypertensive rats related to thecalcium metabolism disorder, Brain Res. Bull. 30(1993) 107-113.
[11] K. Akiyama, D. Sutoo, Rectifying effect of exercise
on hypertension in spontaneously hypertensive rats
via a calcium-dependent dopamine synthesizing
system in the brain, Brain Res. 823 (1999) 154-160.
[12] D. Sutoo, T. Matsukura, K. Akiyama, Reduction ofblood pressure with calcium supplementation, Eur. J.
Pharmacol. 183 (1990) 1313-1314.

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