U LT R A C L E A N P O LY P R O P Y L E N E S T O R A G E P L AT E S C ATA L O G U E

INTRODUCTIONSTO R A G E / C O L L E C T I O N – 9 6 -W E L L S H A L LOW RO U N D

Are your plates whiter than white?
Or do they harbour a dirty secret?

96-well shallow round

Deep well polypropylene microplates are commonlyused for sample storage in life science laboratories. An
essential aspect of the manufacture of these plates is
the selection of clean raw materials for injection
moulding. Polypropylene is an inert and heat resistantmaterial, ideal for sample storage. However, all gradesof polypropylene are not the same, for example,
Injection moulding grades of polypropylene often
have high concentrations of chemicals to aid themoulding process.

typical example of the other commercial microplates
tested, which included two major German
manufacturers and several other well-known
suppliers. These variously displayed extra peaks with avariety of retention times across the run, indicating
several different contaminants. Further analysis ofthese extra peaks revealed that they were, indeed,mostly recognized chemicals used as additives in thepolypropylene to assist the injection moulding
process.

This allows rapid production of plates and lower costs,
but may compromise the integrity of samples orcompounds which are stored in such plates forextended periods. The problem is particularly acute
where compounds are stored as solutions in solvents
such as methanol or DMSO, as these excellent
solvents have a tendency to extract from thepolypropylene base material any added extractablecompounds such as mould release agents or polymer
flow improvers.

From this it can be seen that it is essential to select thecorrect grade of polypropylene material for storage
plate manufacture. The full report can be downloaded
from our website.

Porvair Sciences has developed a number ofstorage/collection plates to help in thefields of cell biology, molecular biology, drug
discovery, combinatorial chemistry, screening
and genomics. The plates are manufactured
under clean room conditions and a significant
number are DNase/RNase free. They aremainly made from polypropylene, an inert
material giving heat and solvent resistantqualities. Porvair Sciences offers the largest
combination of well shape, number of wells
and well volume on the market. Each is madeto the ANSI/SLAS format for compatibility
with most readers/washers and automatedequipment.

Porvair Sciences has carried out tests on many
manufacturers’ deep well plates to establish levels of
extractables in each. A typical example is illustrated.
Samples of deep well microplate for testing wereobtained from all the major manufacturers. A new
unused plate was selected from each batch andsubjected to a stream of clean, dry compressed air toremove any particulates that may have accumulated.
Testing for polymer leachate and extractablecontamination was performed by incubating
overnight an appropriate volume of HPLC grade
methanol in three wells in each sample plate. The
methanol was spiked with 10ug/ml of Caffeine as aninternal standard. The plates were sealed with afriction seal and left to stand overnight.

14.7mm height

Porvair Sciences offers three plates with wellcapacities of 350µl, 270µl and 220µl. They
have flat-, round- or V-bottoms and can beused for compound storage and culturing.

l Manufactured in polypropylenel Alphanumeric grid referencel Round and V-bottom allow greaterliquid removal and particulate
collectionl Raised rims improve sealing andstop cross contamination

After overnight incubation, 1ul aliquots of each well
sample were subjected to analysis on a GC-MS
system using splitless injection at 250°C.
Separation was performed on a capillary column using
the appropriate temperature gradient. Detection wasby positive ion EI-MS.
In order to simplify the full data set here, results fromeach of the three wells per plate tested have beencombined and averaged.
Results from the GC-MS showed good performancefrom the Porvair microplate, with a clean caffeine
peak and no significant levels of leachable orextractable compounds. Plate B shown here, is a2

W W W. M I C R O P L A T E S . C O M

ALL DIMENSIONS IN MM

Storage plates round
Rim and bottom well shape

Material

Well volume

SterileQuantity/caseCat. no.

No rim, flat

Polypropylene

350µl

–

100

208003Raised-round

Polypropylene

270µl

–

100

209003Raised-V

Polypropylene

220µl

–

100

210003

W W W. M I C R O P L A T E S . C O M

3

STO R A G E / C O L L E C T I O N – 9 6 -W E L L D E E P S Q UA R E

STORAGE/COLLECTION – DEEP WELL ROUND

96-well deep square

Deep well round 1ml

44mm height

42mm height

The family of 96 square well plates was
designed so that it would make the interchangeof plates simpler in automated systems. All
three plates have the same geometry and theonly variable is the well depth.

The plates are made in virgin polypropylene
to minimise extractables. Working volume is
1ml per well and total volume is 1.1ml.
Coloured plates aid identification whenretrieving from storage. Black plates are
suitable for storing light sensitive compounds.

l Made from virgin polypropylenel Manufactured from pre-testedl Tested for low extractables

polypropylene for low extractablesl V bottom to allow total liquidl Alphanumeric grid-referencing

removal, partial collection and to
aid re-suspensionl DNase/ RNase freel Packed in sealed sleeves of 5 plates

l No inner edges to allow bettercollection of magnetic beadsl Rimmed version to stop crosscontamination and enable a better
seal

l DNase/ RNase freel Working volumes of 350µl, 1ml,l Non rimmed version to allow

and 2mlinsertion of vials, or where the seal
is not cruciall Raised well rims to improve heatsealing

NO RIMl Cylindrical well with round bottoml Conical base which aids sample

for optimal mixing and recovery

concentration, reconstitution andcentrifugationl Very easy to use with automatedsample handling systemsl Sterile and non-sterile versionsl Can be stored at -80ºC

availablel Toughened genomics version forseed and leaf grinding
Drawing of 500066 available on request.

Storage plates 96 square wellDescription

4

WITH RIM
Well volume

SterileUse cap mat

Polypropylene, pyramid bottom

2.0ml

–

21900450219009Polypropylene, pyramid bottom, sterile, inner bag of 5

2.0ml

3

21901950219027

Polypropylene, pyramid bottom, toughened for genomics

2.2ml

–

21900450219030Polypropylene, pyramid bottom, toughened for genomics

2.2ml

3

21900450219031

Rim and bottom well shape

ColourWell volume

SterileUse cap mat

Quantity/caseCat. no.

Polypropylene, pyramid bottom

1.0ml

–

21900450219008Raised- round

Natural

1ml–

21903650219002Polypropylene, pyramid bottom, sterile, inner bag of 5

1.0ml

3

21901950219026Raised- round

Natural

1ml

321904250219012Polypropylene, pyramid bottom

350µl

–

21900450219006

No rim- round

Natural

1ml–

21903650219037

Polypropylene, pyramid bottom, sterile, inner bag of 5

350µl

3

21901950219025Raised- round

Black

1ml

–

21903650219412

W W W. M I C R O P L A T E S . C O M

Quantity/case Cat. no.

Polypropylene storage plates round

W W W. M I C R O P L A T E S . C O M

5S T O R A G E / C O L L E C T I O N – 9 6 -W E L L D E E P R O U N D ‘ C O M M O N WA L L’

96-well deep round‘common wall’

STO R A G E / C O L L E C T I O N – 9 6 -W E L L RO U N D LOW P RO F I L E

96-well round low profile
A new introduction to the Porvair range is a low
profile 1.2ml 96-well round plate with a height ofjust 27mm. This allows more plates to be stored or
stacked in a given space. The plates are made withextractable-free polypropylene for excellent results.
A specific anti-evaporation cap mat is also available
for this plate manufactured from thermoplastic
elastomer.

45mm height
These revolutionary plates are made in virgin
polypropylene to minimise extractables. Maximum
volume is 2.075ml per well and working volume isa useful 1.85ml, more than any other comparable‘2ml Round Well’ plate in this class. The ‘common
wall’ design allows the highest possible volume to
be used whilst maintaining an overall height of just
45mm, complete with ANSI/SLAS standard baseand footprint. Extra working volume is thus
assured in a convenient round bottom, round wellformat.

l Allows stacking in higher densitiesl Minimises space needed forcompound storagel Pure virgin polypropylene
l Matching snug-fitting cap matl Manufactured from pre-tested

polypropylene for low extractablesl Alphanumeric grid-referencing
l DNase/ RNase freel Packed in sealed sleeves of 5 platesl Cylindrical well with round bottom

for optimal mixing and recovery
l Very easy to use with automatedsample handling systemsl Can be stored at -80ºCl Easily sealed with matching Cap Mator heat seall Fits Waters AcQuity™ autosamplers

96-well round low profile

96-well deep round ‘common wall’
Pk/QtyPart No.

Description

96 Deep well, 2ml/well Polypropylene round well rimless DNA/RNAase free, inner bag of 55021902096-well round low profile plate

96 Deep well, 2ml/well Sterile Polypropylene round well rimless DNA/RNAase free, inner bag of 550219021

Description

6

W W W. M I C R O P L A T E S . C O M

Working well vol

SterileQty/caseCat. no.

1.1ml

–

50

219250W W W. M I C R O P L A T E S . C O M

7

STO R A G E / C O L L E C T I O N – 3 8 4 -W E L L

STORAGE/COLLECTION – LARGE VOLUME

384-well square

Large volume deep well plates

Porvair Sciences has a selection of 384-wellpolypropylene plates to suit most applications.
All of them are made from pre-tested
polypropylene in Class 100000 clean rooms.

The range of large volume plates is designed to meet special
requirements. Focused on the combinatorial, environmental
and food technology markets, it allows large volumes ofsamples to be transported in recognised ANSI/SLAS format
microplates, to allow greater automation. Plates will accept
the Porvair universal lid (see page 55). All plates are
manufactured from virgin polypropylene, can be heat sealed
and stored for prolonged periods at -80ºC.

l Manufactured for high density

sample collection / storagel Two sizes: 58µl and 300µl per well

working volume

24-well features:l 24 wells with a working capacity of 10ml/welll Standard height (44mm) of a deep well platel Sterile or non sterile versions
l Lidded version available

l Each are designed to allow almost

total removal of liquid sample
l Extra flat – allows plate sealingl Storage temperature down to –80ºCl Available sterile and non sterilel All manufactured to ANSI/SLAS

specifications

48-well features:l Two versions:l 5ml/well, 44mm highl 7ml/well, 68mm highHigh volume deep well microplates
Well shape, bottom shape

No of wells

Working well vol

SterileLid

Qty/ caseCat. no.

Rectangle, V-bottom

24

10ml

–

–

25

360013

Rectangle, V-bottom (bulk pack)

24

10ml

3

–

25

360115

Rectangle, V-bottom

24

10ml

–

3

25

360077

Rectangle, V-bottom (single pack)

24

10ml

3

3

25

360079Rectangle, V-bottom (with bar code)

24

10ml

3

3

25

360080

Rectangle, round-bottom

24

10ml

–

–

25

360117

Rectangle, V-bottom

48

5ml

–

25

360002

Rectangle, V-bottom

48

7ml

–

30

360004l Available for the 2ml and 1ml squareand 2mm round deep well plates
l Easily fitted below platesl Made from impact resistant silicone

Centrifuge support plate
The support plate is designed to fit into the skirt of the deep
well plates. This then allows the plate to be used in acentrifuge to go 3,500G or GenoGrinder® without the risk ofthe plate distorting and causing the well to leak.

Deep well microplate accessories
Storage plates 384-wellQuantity/pack size

Cat. no.

Working well vol

SterileQty/caseCat. no.

Centrifuge support plates for 219008 & 219026

2500150

Square-round

58µl

–

60

224001

Centrifuge support plates for 219009 & 219027

2500114

Square-V

300µl

–

48

219040Centrifuge support plates for 219020 & 219021

2500180

Square-V

300µl

3

48

219041

Centrifuge support plates for 360004

2500212

Well shape, top & bottom

8

Description

W W W. M I C R O P L A T E S . C O M

W W W. M I C R O P L A T E S . C O M

9S T O R A G E / C O L L E C T I O N – G R OW T H P L AT E S

Bacti-growth plates

STORAGE/COLLECTION – FRICTION SEALSl Plate well configuration available from 24- throughBacti-growth plates are specially packaged
plates to allow the growth of bacteria, yeast,
mammalian or insect cell lines.
Using only the highest-grade raw materials,
our plates are tested to ensure no moulding
or polymer contaminants leach out into thesamples. This guarantees reproducibility andmaintains customer confidence. Each Bactigrowth plate consists of a deep well plate anda tight fitting lid, packed sterile in bags.

to 384-well

Friction seals

l Well volumes from 300µl through to 10ml per well

219003l Round and square well available
l Sealed in sterile bags of 5 platesl Robot friendlyl Manufactured to the highest specificationsl Conform to the ANSI/SLAS format

996050MR-96

229090219101219102360080219251219004

Bacti-growth plates
Description

SterileLid

Qty/Pack

Cat. no.

24 x 10ml square wells, polypropylene with lid

3

3

25

36008096 x 1ml round wells, polypropylene with lid

3

3

25

21910196 x 2ml square wells, polypropylene with lid

3

3

25

21910296 x 2ml round wells, polypropylene with lid

3

3

25

219022219004229091360006

Friction seals (cap mats)Description
Silicone sealing mat to fit 384-well plates

Cross reference to Porvair Sciences plates Qty/pack Cat. no.
219040, 219041

50

380001

EVA sealing mat to fit 96-square well plates

219006, 219008, 219009

50

219004

Plate lids

EVA sealing mat to fit 96-square well plates, sterile

219025, 219026, 219027

50

219019Pierceable Santoprene mat for 96-square well plates

219006, 219008, 219009

100

360010

Polystyrene and polypropylene lids are available for all 96-well SBS standard plates. See 229125 and 229128 in
the table opposite.

EVA sealing mat for 96-round well plates

219002, 219032

50

219036

EVA sealing mat for 96-round well plates, black

219002, 219032

50

219038

EVA sealing mat for 96-round well plates, sterile

21901250219042

Pierceable Santoprene seal strip to fit 24-well plates

360013100

360014

Pierceable Santoprene seal mat to fit 48-well plates

360002, 360004

100

360006

Pierceable Santoprene seal strip to fit 96-well plates

360001400

360007

Pierceable sealing cap, round 96-well21900250219044

Mat for round 96-well plates (standard silicone)

219002 and round well plates

5229090

Mat for square 96-well plates (standard silicone)

229230 and square well plates

5229091

Mat for round 96-well plate (PTFE coated silicone)

219002 and round well plates

5229092

Mat for square 96-well plate (PTFE coated silicone)

229230 and square well plates

5229093Polystyrene clear lid for all 96-well SBS standard plates

Fits all plates

100229125

Polypropylene lid for all 96-well SBS standard plates

Fits all plates

100229128Cap mat for 1.1ml low profile storage plate

2192505219251

96-well PCR sealing mat, natural

For rimless PCR plates

5028611396-well PCR sealing mat, rubber, natural

For rimless PCR plates

50286114–1250

286116

8-well PCR sealing strip, 125 strips per pack, 10 packs per case10

W W W. M I C R O P L A T E S . C O M

W W W. M I C R O P L A T E S . C O M

11S T O R A G E / C O L L E C T I O N – D E E P W E L L P L AT E S E L E C T I O N G U I D E

Deep-well plates selection guide
Porvair Sciences is a leading manufacturer of highquality polypropylene deep-well microplates forapplications including compound storage, fraction
collection, sample mixing and preparation. Using only
virgin extractable-free grades of polymer in the
production process ensures that you get reliablereproducible results free from contamination eachtime. This guide will suggest the best deep-well plate
options for your particular areaof research.

When selecting round-well plates, it is important toconsider whether sealing will be accomplished by anadhesive seal, a friction seal (or cap mat) or throughthermal sealing with a welded foil. In the case ofadhesive seals, rimless plates with a completely
smooth top surface are preferable. For friction seals,
either rimmed or rimless work best, whereas for heat
weld sealing, a raised rim is essential. This does notreally apply to square-well plates as the divisions
between wells act like rims anyway.

Polypropylene is a naturally-opaque white polymer
which in its raw untreated state is intrinsicallyhydrophobic and offers a medium-low bind surface
for proteins and peptides. In addition, it also acts as a
low-attachment surface for adherent cells. These
properties can be modified, or enhanced, by further
treatment, for example, coating with very low
binding compounds.

The flow chart on the following pages is designed tohelp you select the correct plate foryour application.

Polypropylene can be sterilised using gamma
radiation, by treatment with ethylene oxide or by
autoclaving at 121ºC. However, as the melting pointof pure homopolymer is 171ºC, and the commerciallyavailable grades 160 – 166ºC, autoclaving ofpolypropylene deep-well plates is not recommended.
This is because some softening of the polymer will beobserved and this can lead to distortion of the plate
structure and consequent deviation from the strict
ANSI/SLAS microplate dimensions. Ethylene oxide
sterilisation can also be problematic due to theoxidative nature of the process which can transformthe natural hydrophobic surface into a stronglyhydrophilic surface.
Deep-well plates can have various geometries
depending on the shape of the well and also the
profile of the well bottom. The most common
geometries are square-well plate with pyramid, or Vshape, bottoms and round-well plates with roundbottoms. The advantage of the square V-well is very
high recovery of compounds from these low-deadvolume plates. Whilst round-well plates exhibit higher
dead volumes, they do allow for excellent mixing andare useful in bead-beating applications where beads
might otherwise get stuck in the tight bottom of a Vwell.

S T O R A G E / C O L L E C T I O N – D E E P -W E L L P L AT E S

96-well deep square
Description

12

W W W. M I C R O P L A T E S . C O M

SterileUse cap mat

Qty/caseCat. no.

Polypropylene, pyramid bottom

2.0 ml–21900450219009Polypropylene, pyramid bottom, sterile, inner bag of 5

2.0 ml321901950219027

Polypropylene, round bottom

1.6 ml–21900450

500066

Polypropylene, pyramid bottom

1.0 ml–21900450219008Polypropylene, pyramid bottom, sterile, inner bag of 5

1.0 ml321901950219026

Polypropylene, pyramid bottom

350 µl–21900450219006

Polypropylene, pyramid bottom, sterile, inner bag of 5

350 µl321901950219025

Polypropylene, pyramid bottom, seed genomics

2.0 ml–21903350219030Polypropylene, pyramid bottom, seed genomics, sterile, inner bag of 5

2.0 ml321901950219031

SterileUse cap mat Qty/case Cat. no.

96-well deep round
Rim and bottom well shape

Colour Well volume

Polypropylene, raised-round DNA/RNAase free, inner bag of 5

Natural

1 ml–

21903650219002Polypropylene, raised-round DNA/RNAase free, inner bag of 5

Natural

1 ml

321904250219012Polypropylene, rimless-round DNA/RNAase free, inner bag of 5

Natural

1 ml–

21903650219037

Polypropylene, raised-round DNA/RNAase free, inner bag of 5

Blue

1 ml

–

21903650219432Polypropylene, raised-round DNA/RNAase free, inner bag of 5

Red

1 ml

–

21903650219422Polypropylene, raised-round DNA/RNAase free, inner bag of 5

Black

1 ml

–

21903650219412Polypropylene, rimless-round DNA/RNAase free, inner bag of 5

Natural

2 ml–

21902050219020Polypropylene, rimless-round DNA/RNAase free, inner bag of 5

Natural

2 ml

321902150219021

96-well deep round ‘common wall’
Description

Qty/pack

Part No.

96 Deep well, 2 ml/well, Polypropylene round well rimless, DNA/RNAase free, inner bag of 55021902096 Deep well, 2 ml/well, Sterile, Polypropylene round well rimless, DNA/RNAase free, inner bag of 550219021

96-well shallow round
Rim and bottom well shape

Material

Well volume

SterileQty/caseCat. no.

No rim, flat

Polypropylene

350 µl–100

208003Raised-round

Polypropylene

270 µl–100

209003Raised-V

Polypropylene

220 µl–100

210003Large volume microplates
Well shape, bottom shape

No of wells

Working well volume

SterileLid

Qty/caseCat. no.

Rectangle, V-bottom

24

10 ml

–

–

25

360013

Rectangle, V-bottom (bulk pack)

24

10 ml

3

–

25

360115

Rectangle, V-bottom

24

10 ml

–

3

25

360077

Rectangle, V-bottom (single pack)

24

10 ml

3

3

25

360079Rectangle, V-bottom (with bar code)

24

10 ml

3

3

25

360080

Rectangle, round-bottom

24

10 ml

–

–

25

360117

Rectangle, V-bottom

48

5 ml

–

25

360002

48

7 ml

–

30

36000424/48/96

–

–

100

229125

Rectangle, V-bottom

The height of a deep-well plate will normallydetermine the maximum volume, but in recent years
space has been saved through the use of ‘common
wall’ designs in round-well format that use larger
diameter wells to reduce height and maximise
volume. These designs can also allow more plates tobe fitted into equipment such as HPLC autosamplers,
incubators and the like.

Well volume

Polystyrene Universal SLAS Lid

3384-well
Well shape, top & bottom

Working well volume

SterileQty/caseCat. no.

Square-round

58 µl

–

60

224001

Square-V

300 µl–48

219040

Square-V

300 µl3

48219041W W W. M I C R O P L A T E S . C O M

13MICROPLATESMICROPLATES

96-WELL
96-WELL
96-WELL
24/48-WELL
384-WELLMICROPLATESM I C R O P L AT E S
M I C R O P L AT E SBlack – for light sensitive
operations 219412

Blue – for easy
identification 219422

Natural – 219002

Red – for easy
identification 219422

CHOOSE STERILITY

Sterilsed by gamma radiation 219041

CHOOSE STERILITY

300 μl per well

CHOOSE VOLUME OF RECTANGLE-WELL

Unsterilised 219040

Unsterilised 360013

24 x 10 ml square-well

Sterilsed by gamma radiation 360115

48 x 5 ml rectangle-well non-sterile 360002

CHOOSE VOLUME OF RECTANGLE-WELL

For bead-beating 219030

CHOOSE TYPECHOOSE TYPE

For everyday use 219009

Unsterilised

Sterilsed by gamma radiation

For bead-beating 219031

58 μl per well, not sterile 224001

48 x 4.8 ml square-well, non-sterile 360096

OR >4.8 ml <10 ml

For everyday use 219027

Unsterilised 219020

CHOOSE STERILITYCHOOSE STERILITY

Sterilsed by gamma radiation 219021

Square-well, V-bottom – good recovery

CHOOSE WELL SHAPE

Unsterilised

Unsterilised 219008

CHOOSE COLOUR & TYPE

Sterilsed by gamma radiation 219026

Round-well, round bottom – good mixing

OR >1 ml <2 ml - use a 2.2 ml plate

Sterilised by gamma
radiation, natural PP 219012

CHOOSE STERILITY

RimmedCHOOSE RIM

Common wall rimless,natural PP 219250
Rimless, natural PP
non-sterile 219037

44mm high

CHOOSE STERILITYCHOOSE HEIGHT

27mm high1 ml square-well, V-bottom – good recovery

Unsterilised 219006

CHOOSE STERILITY

350 μl V-well –high recovery 45mm

1.1 ml round-well, round bottom – good mixing

CHOOSE WELL SHAPE

Sterilsed by gamma radiation 219025

270 μl V-well –high recovery 14mm high 210003

CHOOSE THE WELL BOTTOM

Flat-well bottom –highest volume 14mm high 208003

OR >500 µl <1 ml – use a 1.1 ml plate

Round bottom –good mixing 14mm high 209003<500 µl – use a 350 µl plate

CHOOSE THE NUMBER OF WELLS, OR PLATE FORMAT YOU NEED

© Porvair Sciences 2017

P/N 420004 © Porvair Sciences Limited 2017.
Produced by Cream Ink Limited www.creamink.com
Printed in the UK.

Accounts
Porvair plc
7 Regis Place
Bergen Way Kings Lynn
Norfolk UK PE30 2JW
Tel: 44 (0)1553 765500
Fax: +44 (0)1553 765599

Customer Services and Sales Office
Porvair Sciences Ltd
Clywedog Road South
Wrexham Industrial Estate
Wrexham North WalesUK LL13 9XS
Tel: +44 (0) 1978 666240/666239
Fax: +44 (0) 1978 660007
email: int.sales@porvair-sciences.com
www.microplates.com

Porvair Sciences offers a complete range of microplates for storage, collection, assays, ELISA and tissue
culture. For detailed information on our other products, including tissue culture plastics and ourrevolutionary CAIP Chromatin preparation kits, please request our other brochures or visit our website
www.microplates.com
Porvair Sciences catalogue of microplates& microplate equipment (70 pages)

C ATA LO G

CROPLUE OF MIICROPLAT E S & M

IPME
AT E E Q U

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ITION
SIXTH ED

Porvair Sciences chromatography
supplies catalogue (28 pages)

Porvair Sciences assay plates
brochure (16 pages)
C H R O M ATO G R

APHY AND SAMPLE PREPARAT I O NC ATA LO G U E
FIRST EDITIO
N

ASSA

Y PL
AT E

S