ᐔᚑ 26 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯⎇ⓥႎ๔ᦠᶏ⮺↱᧪⢽⾰ߦ߅ߌࠆ᛫Ἳ∝૞↪ߩ⸃᣿ߣߘߩᵴ↪ (╙ 3 ႎ)-⫳⊒శᢔੂᬌ಴-HPLC ߦࠃࠆࠬࠨࡆࡁ࡝↱᧪࡝ࡦ⢽⾰ߩቯ㊂㘩ຠᎿᬺㇱᨷᬀ࿻੺ 㢬↰⵨⟤ศ᧛⤿ผ⫳⊒శᢔੂᬌ಴-­HPLC ᴺࠍ↪޿ߡ㧘᛫Ἳ∝૞↪߇ᦼᓙߐࠇࠆࠬࠨࡆࡁ࡝↱᧪࡝ࡦ⢽⾰
㧔NPL㧕ߩಽ೎ቯ㊂ࠍ⹜ߺߚ㧚Diol ࠞ࡜ࡓࠍ↪޿ߚ㗅⋧ಽ㈩ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ࠍㆡ↪ߒߡಽᨆ♽ࠍ⏕┙ߒ㧘sn-­1 ૏ߦ palmitic  acid ߇㧘sn-­2 ૏ߦ oleic  acid ߇⚿วߔࠆᮡḰ࡝ࡦ⢽⾰
ࠢ࡜ࠬ 6 ⒳㘃㧔phosphatidylglycerol;;  PG㧘phosphatidylethanolamine;;  PE㧘phosphatidylcholine;;  PC,  phosphatidylserine;;  PS,  phosphatidic  acid;;  PA ෸߮ lysophosphatidylcholine;;  LPC㧕ࠍ↪޿ߚࡃ࡝࠺࡯࡚ࠪࡦࠍታᣉߒߚ㧚ߎࠇࠄߩᮡḰ࡝ࡦ⢽⾰ߦ߅޿ߡߪ㧘10  mg/mL એ਄ߩỚᐲߢᬌ㊂✢ߩ⋥✢ᕈ߇ᓧࠄࠇ㧘ಽᨆߩౣ⃻ᕈ߽⦟ᅢߢ޽ߞߚ㧚ᰴߦ㧘ᧄಽᨆᴺߦ߅ߌࠆ NPL ߣᮡḰ࡝ࡦ⢽⾰ߩࠢࡠࡑ࠻ࠣ࡜ࡓࠍᲧセߒߚߣߎࠈ㧘NPL ߦ߅޿ߡᮡḰ࡝ࡦ⢽⾰ߩ PG,  PC ෸߮ LPCߦ⋧ᒰߔࠆࡇ࡯ࠢߣหߓ଻ᜬᤨ㑆ߦࡇ࡯ࠢ߇ṁ಴ߖߕ㧘ߎࠇࠄߩ࡝ࡦ⢽⾰ࠢ࡜ࠬߩหቯ߇࿎㔍ߢ޽ߞߚ㧚ߎߩ଻ᜬᤨ㑆ߩߕࠇߪ㧘NPL㧘ᮡḰ࡝ࡦ⢽⾰ߘࠇߙࠇߦ⚿วߔࠆ⢽⢌㉄ߩ⒳㘃߇⇣ߥࠆߚ߼ࠞ࡜ࡓౝߢߩಽ㈩᜼േ߇⇣ߥࠆߎߣߦ⿠࿃ߔࠆߣ⠨߃ࠄࠇߚ㧚ߒߚ߇ߞߡ㧘NPL ߦ฽߹ࠇࠆ࡝ࡦ⢽⾰ࠢ࡜ࠬߩಽ೎ቯ㊂ߦኻߒߡᧄಽᨆᴺࠍㆡ↪ߔࠆߚ߼ߦߪ㧘  NPL ߩ࡝ࡦ⢽⾰ߣᭂᕈ߇㘃ૃߒߚ߽ߩࠍᮡḰ‛⾰ߣߒ㧘଻ᜬᤨ㑆ߩߕࠇࠍᭂജߥߊߔᎿᄦ߇ᔅⷐߣ್ᢿߐࠇߚ㧚    1. ߪߓ߼ߦᚒ‫ߪޘ‬㧘ߎࠇ߹ߢߦࠬࠨࡆࡁ࡝↱᧪ⶄว⢽⾰ߦ߅
޿ߡ LPS ೝỗߦࠃࠆࡑࠢࡠࡈࠔ࡯ࠫߩᵴᕈൻࠍᛥ߃ࠆ૞↪㧔᛫Ἳ∝૞↪㧕߇޽ࠆߎߣࠍ᣿ࠄ߆ߦߒߡ߅

߽ႎ๔ߐࠇߡ޿ࠆ㧚  ߎߩࠃ߁ߥ⢛᥊߆ࠄ㧘ࡁ࡝ߩ᛫Ἳ∝૞↪ࠍᵴ߆ߒߚᯏ⢻ᕈ㘩ຠ⚛᧚ࠍ㐿⊒ߔࠆ߁߃ߢ㧘ࠬࠨࡆࡁ࡝ේ
ᢱߦ฽߹ࠇࠆ NPL ߩ฽㊂߿࡝ࡦ⢽⾰ࠢ࡜ࠬߩ⚵ᚑࠅ 1),2)㧘ᯏ⢻ᕈ㘩ຠ⚛᧚ߣߒߡߩࠬࠨࡆࡁ࡝ߩᵴ↪߇ᦼᓙߐࠇߡ޿ࠆ㧚ߎߩⶄว⢽⾰ߪ㧘ࠬࠨࡆࡁ࡝ߦੇ
῎㊀㊂޽ߚࠅ 5%⒟ᐲ฽߹ࠇ 1)   㧘ਥߣߒߡ࡝ࡦ⢽⾰ߣ♧⢽⾰߆ࠄᚑߞߡ߅ࠅ㧘ߘߩ߁ߜ࡝ࡦ⢽⾰↹ಽߪ
phosphatidylglycerol   (PG)㧘phosphatidyl-­   ethanolamine  (PE)㧘phosphatidylcholine   (PC)   ╬ߩⶄᢙߩ࡝ࡦ⢽⾰Ყࠍቯ㊂⊛ߦᛠីߔࠆߎߣߪ߈ࠊ߼ߡ㊀ⷐߢ޽ࠆ㧚ߒ߆ߒߥ߇ࠄ㧘ᓥ᧪߆ࠄ࡝ࡦ⢽⾰ࠢ࡜ࠬߩಽᨆߦ↪
޿ࠄࠇߡ޿ࠆ⭯ጀࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ߦࠃࠆᚻᴺߪ㧘቟ଔߢ◲ଢߢߪ޽ࠆ߽ߩߩ㧘ቯ㊂♖ᐲ߇ૐߊ㧘߹ߚ㧘ಽᨆߩ⥄േൻ߿ㅪ⛯⊛ߥಽᨆߦะ߆ߥ޿ߣ޿߁⍴ᚲ߇޽ߞߚ㧚ઁᣇ㧘↢૕ᚑಽߩㅪ⛯ಽᨆ߿⥄േಽᨆߦࠢ࡜ࠬߢ᭴ᚑߐࠇߡ޿ࠆ 2),3)㧚ࠬࠨࡆࡁ࡝↱᧪࡝ࡦ⢽⾰㧔NPL㧕ߪࡑ࠙ࠬ↱᧪ RAW264.7 ⚦⢩ߦ߅ߌࠆ LPS⺃ዉဳߩᵴᕈൻࠍᛥ߃㧘iNOS,  COX-­2,  TNF-­DߥߤߩἻ∝ᕈ‛⾰ߩ⊒⃻⺃ዉࠍᛥ೙ߔࠆ 2)㧚߹ߚ㧘࡝ࡦ⢽⾰ߦ⚿วߔࠆ⢽⢌㉄ߩ⚂ඨᢙ߇ᄙଔਇ㘻๺⢽⢌㉄ߢ޽ࠆ eicosapentaenoic   acid㧔EPA㧕ߢභ߼ࠄࠇ㧘㒽਄᳢↪ߐࠇࠆ㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯㧔HPLC㧕
ߪ㧘⢽⾰ߩ⚡ᄖᵄ㐳㗔ၞߦ߅ߌࠆๆ෼߇ᒙ޿ߚ߼㧘ߎࠇ߹ߢๆశᐲᬌ಴ེߢߪ㜞ᗵᐲᬌ಴߇ߢ߈ߥ߆ߞߚ㧚  ㄭ ᐕ 㧘 ⫳ ⊒ శ ᢔ ੂ ᬌ ಴ ᴺ 㧔 Evaporative   Light  Scattering  Detection;;  ELSD㧕߇⚡ᄖ㗔ၞߢߩๆ෼߇ᒙᬀ‛↱᧪ߩ࡝ࡦ⢽⾰ߦߪ⷗ࠄࠇߥ޿࡙࠾࡯ࠢߥ᭴ㅧࠍ᦭ߒߡ޿ࠆ 3)㧚  ᚒ‫ߚ߹ߪޘ‬㧘᛫Ἳ∝૞↪ߩᒝߐ߇࡝ࡦ⢽⾰ࠢ࡜ࠬߦࠃߞߡ⇣ߥࠅ㧘NPL ࠍ᭴ᚑߔࠆ࡝ࡦ⢽⾰ࠢ࡜ࠬߩਛߢߪ PG ߇․ߦᒝ޿ࡑࠢࡠࡈࠔ࡯ࠫᵴᕈൻᛥ೙૞
↪ࠍ᦭ߔࠆߎߣࠍ᣿ࠄ߆ߦߒߡ޿ࠆ 2)㧚ห᭽ߦ㧘PG޿‛⾰ߩ HPLC ߦᔕ↪ߐࠇߡ޿ࠆ 6)㧚ELSD ߪ㧘ࠞ࡜ࡓ߆ࠄṁ಴ߒߚ⒖േ⋧ࠍ⫳⊒ߐߖࠆߎߣߦࠃࠅ⋡⊛ൻว‛ࠍᓸ☸ሶൻߒ㧘ߘߩᢔੂశࠍ᷹ቯߔࠆᬌ಴ᴺߢ޽ࠆ㧚⒖േ⋧ߣߣ߽ߦ⫳⊒ߒߡߒ߹߁ૐᴣὐൻว‛ࠍ㒰߈   ේℂ⊛ߦ߶ߣࠎߤోߡߩൻว‛ࠍᬌ಴ߔࠆߎߣ߇ߢ߈㧘ๆశᐲᬌ಴ེߢᬌ಴ߢ߈ߥ޿ൻว

߇㧘Mycoplasma   pneumoniae ߩೝỗߦࠃࠆ TLR-­2 ࠍ੺ߒߚ RAW264.7 ⚦⢩ߩࠛࠗࠦࠨࡁࠗ࠼↥↢ࠍᛥ߃ࠆߎߣ߇㧘Kuronuma ࠄ 4)߿ Kandasamy ࠄ 5)ߦࠃߞߡ

‛㧔὇᳓ൻ‛㧘ࠕ࡞ࠦ࡯࡞㧘⢽⾰㧘⇇㕙ᵴᕈ೷㧘࠹࡞ࡍࡁࠗ࠼ߥߤ㧕ߩಽᨆߢᆭജࠍ⊒ើߔࠆ㧚߹ߚ㧘ᓥ᧪ߩ␜Ꮕዮ᛬₸ᬌ಴ࠃࠅ߽ᗵᐲ߇㜞ߊ㧘ើ⊒ᕈߩ䋭 㪉㪈 䋭

ᐔᚑ 26 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯⎇ⓥႎ๔ᦠ

⒖േ⋧ߢ޽ࠇ߫㧘ࠣ࡜ࠫࠛࡦ࠻ಽᨆߦ߽ኻᔕߢ߈ࠆ
㐳ᚲࠍ᦭ߔࠆ㧚  એ਄߆ࠄ㧘ᧄ⎇ⓥߢߪ㧘ELSD-­HPLC ߦࠃࠆ NPLਛߩ࡝ࡦ⢽⾰ࠢ࡜ࠬߩಽ೎ቯ㊂ᴺߩ⏕┙ࠍ⹜ߺߚ㧚ቯ㊂ᴺߩ⏕┙ߦ޽ߚߞߡߪ㗅⋧ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯

chloroform ߪ↪޿ߕ㧘n-­hexane,      2-­propanol ෸߮ waterߩᷙวṁᇦߣߒߚ㧚߹ߚ㧘⒖േ⋧߳ߩᷝട೷ߣߒߡacetic   acid   ෸߮ ammonium   acetate   (1   mol/L   ᳓ṁᶧ)  ࠍ૶↪ߒߚ㧚ߎࠇࠄߩᷙวṁᇦߩᲧ₸߿ᷝട೷ߩỚᐲࠍᄌ߃ߡಽᨆࠍ⹜ⴕߒ㧘ಽ㔌᧦ઙߩᦨㆡൻࠍ⹜ߺߩේℂࠍㆡ↪ߒ㧘sn-­1 ૏ߦ palmitic   acid ߇㧘sn-­2 ૏ߦ oleic  acid ߇⚿วߔࠆᮡḰ࡝ࡦ⢽⾰ࠢ࡜ࠬ 6 ⒳㘃ࠍ↪޿ߡࡃ࡝࠺࡯࡚ࠪࡦࠍታᣉߒߚ㧚ߐࠄߦ㧘ᧄᚻᴺࠍ NPL ਛߩ࡝ࡦ⢽⾰ࠢ࡜ࠬߩಽᨆߦᔕ↪ߒߚߩߢߘߩ⚿ᨐߦߟ޿ߡႎ๔ߔࠆ㧚    ߚ㧚    2. ታ㛎ᣇᴺ2.1 ᧚ᢱ෸߮⹜⮎ࠬࠨࡆࡁ࡝ߣߒߡ 2011 ᐕᐲ᦭᣿ᶏ↥᧼ᶏ⧡ࠍ↪
޿ߚ㧚⹜ᢱࠍ⿥㆙ᔃ☳⎈ᯏ㧔ZM-­1;;   Retsch ⵾㧕ߢ☳⎈ߒ㧘☸ሶᓘ 125   Pm એਅߩᓸ☳ᧃࠍᓧߚ㧚ຠ⾰ߩഠൻࠍ㒐ߋߚ߼㧘ᓸ☳ᧃߪኒኽਅ-­18͠ߢ଻▤ߒߚ㧚  100   Pg/mL㧕ࠍ↪޿ߡ㧘⒖േ⋧ߦ߅ߌࠆ n-­hexane,  2-­propanol ෸߮ water ߩᲧ₸߇࡝ࡦ⢽⾰ࠢ࡜ࠬߩಽ㔌ᐲߦ෸߷ߔᓇ㗀ࠍᬌ⸛ߒߚ㧚ߘߩ⚿ᨐ㧘࿑㧝ߦ␜ߔࠃ߁ߦ㧘ᷙวṁᇦߩᲧ₸ߪ࡝ࡦ⢽⾰ࠢ࡜ࠬߩಽ㔌ᐲߦᄢ߈ߊᓇ㗀ߒ㧘n-­hexane ߩᲧ₸߇㜞ߔ߉ࠆߣฦ‫ޘ‬ߩ࡝ࡦ⢽⾰ࠢ࡜ࠬ߇චಽಽ㔌ߖߕ㧘ㅒߦૐߔ߉ࠆߣᧄ ⎇ ⓥ ߦ ߅ ߌ ࠆ ⹜ ⮎ ߪ એ ਅ ߩ ߽ ߩ ࠍ ↪ ޿ ߚ 㧦     1-­palmitoyl-­2-­oleoyl-­phosphatidylglycerol  (PG),  1-­palmitoyl-­2-­oleoyl-­phosphatidylethanolamine   (PE),     1-­palmitoyl-­2-­oleoyl-­phosphatidic  acid  (PA),        1-­palmitoyl-­2-­oleoyl-­phosphatidylserine  (PS),  1-­  palmitoyl-­2-­oleoyl-­phosphatidylcholine  (PC),  1-­     ࠞ࡜ࡓߦ଻ᜬߐࠇߔ߉ߡಽᨆᤨ㑆ߩㆊᄢࠍ᜗ߊ⚿ᨐߣߥߞߚ㧚᧦ઙᬌ⸛ߩ⚿ᨐ㧘ᦨㆡߥಽ㔌᧦ઙߣߒߡ㧘n-­hexane/2-­propanol/water/acetic   acid   =   63/34/3/0.07  3. ⚿ᨐ෸߮⠨ኤ  3.1 ⒖േ⋧ߦ߅ߌࠆ n-hexane/2-propanol/water/acetic acid ߩᲧ₸  ࡝ࡦ⢽⾰ᷙวᮡḰᶧ㧔PG,  PE,  PA,  PS,  PC,  LPC;;   ฦ(v/v)   ࠍㆬᛯߒߚ㧚

LSU60palmitoyl-­2-­hydroxy-­phosphatidylcholine  (LPC)  (એ਄ 6ὐ ✚ ߡ Avanti   Polar   Lipid),   chloroform,   n-­hexane,  2-­propanol,  acetic  acid,  1  mol/L  ammonium  acetate ᳓ṁᶧ   (એ਄ 5 ὐ✚ߡ HPLC  grade,   ๺శ⚐⮎Ꮏᬺ).     2.2 ᮡḰ‛⾰෸߮ᬌ૕ߩ⺞⵾
ฦ⒳࡝ࡦ⢽⾰ߪ 10   mg/mL   chloroform ṁᶧߣߒߡ4020LPCPELSU80PC604020PGPA0PE8060PCPG

4020n -­‐Hexa ne2-­‐Propa nolWa terAceti c  a ci d544240.06n -­‐Hexa ne
2-­‐Propa nol
Wa terAceti c  a ci d

63
34
30.07

PS0LSUኒኽਅ-­40͠ߦߡ଻▤ߒ㧘ಽᨆ⋥೨ߦ⒖േ⋧ߢᚲቯߩỚᐲߦᏗ㉼ߒߡ૶↪ߒߚ㧚  ߹ ߚ 㧘ᣢ ႎ ߦḰ ߓ 2) 㧘ࠬ ࠨ ࡆ ࡁ࡝ ᓸ ☳ᧃ ࠃ ࠅchloroform/methanol   (1/1,v/v)ߢ⢽ṁᕈ↹ಽࠍ᛽಴ᓟ㧘࿕⋧᛽಴ᴺߦࠃࠅ࡝ࡦ⢽⾰↹ಽࠍಽ㔌ߒߚ㧚ߎࠇࠄߩ↹ಽߪ chloroform/methanol   (2/1,v/v) ߦṁ⸃ߐߖ

PEPC+PG
PA80LPCPSn -­‐Hexa ne
2-­‐Propa nol
Wa terAceti c  a ci dPA00  24681012 14 16 18 20
Rerention time 䋨min䋩

72
26
20.08

2224262830  HPLC ߦ߅ߌࠆ⒖േ⋧ߩᷙวṁᇦߩᲧ₸߇  ࡝ࡦ⢽⾰ࠢ࡜ࠬ   ߩಽ㔌ᐲߦ෸߷ߔᓇ㗀     ߡኒኽਅ-­40͠ߢ଻▤ߒ㧘ಽᨆ⋥೨ߦ⒖േ⋧ߢᚲቯߩỚᐲߦᏗ㉼ߒߡ૶↪ߒߚ㧚  2.3 HPLC ᧦ઙ  HPLC ߩ᧦ઙᬌ⸛ߦ޽ߚࠅ㧘એਅࠍၮᧄ᧦ઙߣߒߚ㧦ⵝ⟎,   Waters ⵾ UPLC;;   ࠞ࡜ࡓ,   Develosil   100  Diol-­5   (I2   x   250   mm,   ㊁᧛ൻቇ);;   ᵹㅦ,   0.22   mL/min;;  ࿑㧝              ࠞ࡜ࡓ᷷ᐲ,  40͠;;   ᵈ౉㊂,  5  PL;;  ELSD ࠟࠬ࿶,  25  psi,  ELSD ࡀࡉ࡜ࠗࠩ࡯᷷ᐲ,   40͠;;   ELSD ࠼࡝ࡈ࠻࠴ࡘ࡯ࡉ᷷ᐲ,  50͠㧚  ⒖േ⋧ߦߟ޿ߡߪ㧘ⵝ⟎ߩ㈩▤ߦኻߔࠆ࠳ࡔ࡯ࠫ߿ⅣႺߦਈ߃ࠆᖡᓇ㗀ࠍ⠨ᘦߒߡᲥᕈߩᒝ޿

3.2 ࠕࡦࡕ࠾࠙ࡓࠗࠝࡦᷝടߩᓇ㗀  3.1 ߢ᳿ቯߒߚಽ㔌᧦ઙߦߡಽᨆࠍ⹜ⴕߔࠆਛߢ㧘PG,  PA,  PS ߩ଻ᜬᤨ㑆߇㧘PE,  PC,  LPC ߣᲧߴߡ቟ቯߖߕ㧘ᵈ౉ౣ⃻ᕈ߇ૐߊߥࠆ⃻⽎߇⹺߼ࠄࠇߚ㧔࿑㧞㧕㧚ߎࠇࠄߩ࡝ࡦ⢽⾰ࠢ࡜ࠬߪᵈ౉࿁ᢙࠍ㊀ߨࠆߦ䋭 㪉㪉 䋭PE;;  phophatidylethanolamine,  PC;;  phosphatidylcholine,  PG;;     phosphatidylglycerol,  PA;;  phosphatidic  acid,  LPC;;  lysophosphatidylcholine,  PS;;  phosphatidylserine        ᐔᚑ 26 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯⎇ⓥႎ๔ᦠ䋭 㪉㪊 䋭ᐔᚑ 26 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯⎇ⓥႎ๔ᦠ䋭 㪉㪋 䋭ᐔᚑ 26 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯⎇ⓥႎ๔ᦠߎߣࠍ⋡⊛ߣߒߡ㧘ELSD ࠍ↪޿ߚ HPLC ߦࠃࠆಽᨆᚻᴺߩ⏕┙ࠍ⹜ߺߚ㧚ߘߩ⚿ᨐ㧘  1)  Diol ࠞ࡜ࡓࠍ↪޿ߚ㗅⋧ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ߩේℂࠍㆡ↪ߒ㧘6 ⒳㘃ߩᮡḰ࡝ࡦ⢽⾰ࠢ࡜ࠬࠍ⦟ᅢߦಽ㔌ߔࠆಽᨆ♽ࠍ⏕┙ߒߚ㧚  ⮺↱᧪⢽⾰ߦ߅ߌࠆ᛫Ἳ∝૞↪ߩ⸃᣿ߣߘߩᵴ↪  (2)   -­ࠬࠨࡆࡁ࡝ߦ߅ߌࠆ᛫Ἳ∝ᕈ⢽⾰ߩ․ቯ-­.   ᐔᚑ 25 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯ႎ๔,   19-­28  (2014).  
3)   Araki   S,   Sakurai   T,   Omata   T,   Kawaguchi   A   and  2)ᧄಽᨆᴺߦ߅޿ߡߪ㧘߅߅߻ߨ 10㨪100   Pg/mL ߩ▸࿐ߢᮡḰ࡝ࡦ⢽⾰ߩᬌ㊂✢ߩ⋥✢ᕈ߇ᓧࠄࠇ㧘ౣ⃻ᕈ߽⦟ᅢߢ޽ߞߚ㧚  3)ᧄಽᨆᴺࠍ NPL ߩ࡝ࡦ⢽⾰ࠢ࡜ࠬߏߣߩಽ೎ቯ㊂ߦᔕ↪ߒߚ߇㧘PC,  PG ෸߮ LPC ߦߟ޿ߡߪ㧘ߞ଻ᜬᤨ㑆ߩߕࠇ߆ࠄࡇ࡯ࠢหቯ߇ߢ߈ߕ㧘NPL ߩಽ

Murata  N,  Lipid  and  fatty  acid  composition  in  the  red  alga   Porphyra   yezoensis.   Jpn.   J.   Phycol.,   34,   94-­100  (1986).  
4)   Kuronuma   K,   Mitsuzawa   H,   Takeda   K,   Nishitani   C,  Chan   ED,   Kuroki   Y,   Nakamura   M   and   Voelker   DR,  Anionic   Pulmonary   Surfactant   Phospholipids   Inhibit  ᨆ♽ߣߒߡߪਇචಽߢ޽ߞߚ㧚  એ਄ߩ⚿ᨐ߆ࠄ㧘੹ᓟ㧘ᧄಽᨆᴺࠍ NPL ߩቯ㊂ಽᨆߦㆡ↪ߔࠆߚ߼ߦ㧘ᮡḰ࡝ࡦ⢽⾰ߩㆬᛯߥߤߩᡷ⦟ߩ૛࿾߇޽ࠆߣ್ᢿߐࠇߚ㧚  ߥ߅㧘ᧄ⎇ⓥߦ૶↪ߒߚ㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈߪ㔚Ḯ┙࿾࿾ၞኻ╷੤ઃ㊄ߩ⵬ഥߦࠃࠆ߽ߩߢ޽ࠆ㧚    Inflammatory   Responses   from   Alveolar   Macrophages  and   U937   Cells   by   Binding   the   Lipopolysaccharide-­  interacting   Proteins   CD14   and   MD-­2.   J.   Biol.   Chem.,  284,  25488-­25500  (2009).  
5)  Kandasamy  P,  Zarini  S,  Chan  ED,  Leslie  CC,  Murphy  RC   and   Voelker   DR,   Pulmonary   Surfactant  ෳ⠨ᢥ₂  1)   ᨷᬀ࿻੺,   㢬↰⵨⟤,   ૒⮮⌀૒ᕺ,   ศ᧛⤿ผ,   ᶏ⮺↱᧪⢽⾰ߦ߅ߌࠆ᛫Ἳ∝૞↪ߩ⸃᣿ߣߘߩᵴ↪  (1)   -­ࠬࠨࡆࡁ࡝ߦ߅ߌࠆ᛫Ἳ∝૞↪ߩត⚝-­.   ᐔᚑ24 ᐕᐲ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯ႎ๔,  1-­9  (2013).  
2)   ᨷᬀ࿻੺,   㢬↰⵨⟤,   ૒⮮⌀૒ᕺ,   ศ᧛⤿ผ,   ᶏ

Phosphatidylglycerol  Inhibits  Mycoplasma  pneumoniae-­stimulated   Eicosanoid   Production   from  Human  and  Mouse  Macrophages.  J.  Biol.  Chem.,  286,  7841-­7853  (2011).  
6)   ኹ↰⧷ᢅ㧘ᣧᎹ⑓ብ㧘ਃ਄ඳਭ㧘⫳⊒శᢔੂᬌ಴ེ ߩ ᦭ ല ⊛ ߥ ૶ ޿ ᣇ 㧘 Chromatography,   32(3),  141-­152  (2011).  

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