iMatrix-511MG
Product No. 892 005 1,050 μg

Version 003
Store at 2-15°C

Product description: iMatrix-511MG is a recombinanthuman laminin-511 E8 fragment protein expressed inChinese Hamster Ovary (CHO)-S cells. iMatrix511MG contains the integrin-binding site of thelaminin-511 molecule. iMatrix-511MG is a useful cell
culture substrate for feeder-free culture and single-cellpassage of ES cells and iPS cells, facilitating stableculture expansion. iMatrix-511MG is also useful forthe culture of other cells adhering to laminin-511.
Content: Recombinant humanfragment protein in PBS(-)

laminin-511

E8Concentration: 0.5 mg/mL
Amount: Product No. 892 005

1,050 μg / 6 tubes
175 μg / 0.35 mL / tube

Storage: Store at 2°C to 15°C, protect from light.
Expiration date: The shelf life is 2 years from the dateof manufacture. The expiration date is printed on theouter carton.
Activity: The dissociation constant (Kd) for the binding
with integrin α6β1 is 1 nM or less.
Methods of use: By either of the following methods,iMatrix-511MG can be coated onto a culture vessel.
The optimum coating density may differ by cell-type,cell-line, medium selected, or purpose. Insufficientcoating density may result in the detachment of cells
and varied cell conditions while the excessive coatingdensity may lead to difficulty in detaching cells forpassage.
A. Pre-coating method
Determine the optimal coating density. 0.5 μg/cm2 is astandard but test between 0.1 and 1.5 μg/cm2.
1) Dilute iMatrix-511MG with PBS(-). Use the diluted
iMatrix-511MG immediately. To coat with 0.5μg/cm2 onto a 6-well plate with 9.6 cm2/well, dilute
9.6 μL of iMatrix-511MG with 2 mL of PBS(-) perwell.
2) Place the diluted iMatrix-511MG into a culture
vessel and incubate either at 37°C for 1 h, or at room
temperature for 3 h, or at 4°C overnight.
3) Aspirate the coating solution. Then, immediately
seed your cells. Do not allow the coated surface todry.

B. Pre-mixing method
Determine the optimal coating density for cell culture.
The standard density is 0.25 μg/cm2 but test between
0.1 and 1.5 μg/cm2. The optimal coating density may beaffected by the medium and cell density of the cellsuspension.
1) Add iMatrix-511MG to the cell suspension. To coatwith 0.25 μg/cm2 onto a 6-well plate with 9.6
cm2/well, add 4.8 μL of iMatrix-511MG to 2 mL ofthe cell suspension per well.
2) Place the cell suspension containing iMatrix511MG into a culture vessel.
*If you face difficulties in detaching cells for passage,
re-adjust the conditions (e.g., reduce the coatingdensity).
References:
Taniguchi Y. et al. (2009), J. Biol. Chem. 284 (12):7820-31
Miyazaki T. et al. (2012), Nat. Commun. 3: 1236
Nakagawa M. et al. (2014), Sci. Rep. 4: 3594
Takashima Y. et al. (2014), Cell 158 (6): 1254-69
Miyazaki T. et al. (2017), Sci. Rep. 7: 41165
Kikuchi T. et al. (2017), Nature 548 (7669): 592-6
Goparaju S.K. et al. (2017), Sci. Rep. 7: 42367
Hayashi R. et al. (2017), Nat. Protoc. 12 (4): 683-96
Ishii K. et al. (2018), Stem Cell Reports 10 (2): 568-82
Caution: In the event of accidental ingestion or contact
with the eyes, immediately wash the affected area andseek medical attention.
Product information: Current information includingreferences and Q&A are available on the website ofMATRIXOME, Inc. Please use the URL or QR code
below.
Designed by: MATRIXOME, Inc.
3-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Institute for Protein Research, Osaka University
Tel: +81-6-6877-0222 Fax: +81-6-6877-0002
Contact: https://matrixome.co.jp/en/contact
URL: https://matrixome.co.jp/en/

Manufactured by: Nippi, Incorporated
1-1-1 Senju Midori-cho, Adachi, Tokyo 120-8601, Japan