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K. Hirayama,
Hirayama T. Ando, R. Takahashi, and A. Murai, A revised primary structure of neocarzinostatin using the combination of endopeptidase, carboxypeptidase Y and fast-atombombardment mass spectrometry, Bull. Chem. Soc. Jpn., 59(5), 1371῍1378 (1986).

2)

K. Hirayama,Hirayama R. Takahashi, S. Akashi, K. Fukuhara, N. Oouchi, A. Murai, M. Arai, S. Murao, K.
Tanaka, and I. Nojima, Primary structure of Paim I, an a-amylase inhibitor from Streptomyces
corchorushii, as determined by the combination of Edman degradation and fast atom bombardment mass spectrometry, Biochemistry, 26(20), 6483῍6488 (1987).

3)

S. Akashi, K. Hirayama,
Hirayama T. Seino, S. Ozawa, K. Fukuhara, N. Oouchi, A. Murai, M. Arai, and S.
Murao, A determination of the positions of disulﬁde bonds in Paim I, a-amylase inhibitor from
Streptomyces corchorushii, using fast atom bombardment mass spectrometry, Biomed. Environ.
Mass Spectrom., 15(10), 541῍546 (1988).

4)

M. Furuya, S. Akashi, and K. Hirayama,
Hirayama The primary structure of human EGF produced by genetic
engineering, studied by high-performance tandem mass spectrometry, Biochem. Biophys. Res.
Commun., 163(2), 1100῍1106 (1989).

5)

S. Akashi, K. Hirayama,
Hirayama A. Murai, M. Arai, and S. Murao, Determination of the primary structureof Paim II, an a-amylase inhibitor from Streptomyces corchorushii, by high-performance tandem
mass spectrometry, Biochem. Biophys. Res. Commun., 158(2), 514῍519 (1989).

6)

H. Miyano, E. Suzuki, S. Akashi, M. Furuya, T. Tsuji, K. Hirayama,
Hirayama and N. Nagashima, Histidine
microenvironment analyses of recombinant human interleukin-2 by fast atom bombardment massspectrometry and proton magnetic resonance spectrometry, Anal. Sci., 5(6), 759῍761 (1989).

7)

K. Hirayama,Hirayama S. Akashi, M. Furuya, and K. Fukuhara, Rapid conﬁrmation and revision of theprimary structure of bovine serum albumin by ESIMS and Frit-FAB LC/MS, Biochem. Biophys.
Res. Commun., 173(2), 639῍646 (1990).

8)

S. Akashi, U. Niitsu, R. Yuji, H. Ide, and K. Hirayama,Hirayama Investigation of the interaction between
enzyme and inhibitor by the combination of chemical modiﬁcation, electrospray ionization mass
spectrometry and frit-fast atom bombardment liquid chromatography/mass spectrometry, Biol.
Mass Spectrom., 22(2), 124῍132 (1993).

9)

S. Murao, K. Ohkuni, M. Nagao, K. Hirayama,Hirayama K. Fukuhara, K. Oda, H. Oyama, and T. Shin,Puriﬁcation and characterization of kumamolysin, a novel thermostable pepstatin-insensitive
carboxyl proteinase from Bacillus novosp. MN-32, J. Biol. Chem., 268(1), 349῍355 (1993).

10)

S. Akashi, K. Noguchi, R. Yuji, U. Tagami, K. Hirayama,Hirayama K. Kato, H. H. Kim, K. Tokioka, I. Shimada,and Y. Arata, Characterization of mouse switch variant antibodies by matrix-assisted laser
desorption ionization mass spectrometry and electrospray ionization mass spectrometry, J. Am.
Soc. Mass Spectrom., 7(8), 707῍721 (1996).

11)

H. Sato, M. Ikeda, K. Suzuki, and K. Hirayama,Hirayama Site-speciﬁc modiﬁcation of interleukin-2 by thecombined use of genetic engineering techniques and transglutaminase, Biochemistry, 35(40),13072῍13080 (1996).

12)

K. Hirayama,Hirayama R. Yuji, N. Yamada, K. Noguchi, Y. Yamaguchi, J. Enokizono, K. Kato, Y. Arata, andI. Shimada, Convenient peptide mapping of immunoglobulin G2b and di#erentiation between
leucine and isoleucine residues by mass spectrometry using 2H-labeled leucine, J. Mass Spectrom.
ῌ 118 ῌSoc. Jpn., 46(1), 83῍89 (1998).
13)

K. Hirayama,Hirayama R. Yuji, N. Yamada, K. Kato, Y. Arata, and I. Shimada, Complete and rapid peptide
and glycopeptide mapping of mouse monoclonal antibody by LC/MS/MS using ion trap mass
spectrometry, Anal. Chem., 70(13), 2718῍2725 (1998).

14)

U. Tagami, S. Akashi, T. Mizukoshi, E. Suzuki, and K. Hirayama,Hirayama Structural studies of the Maillard
reaction products of a protein using ion trap mass spectrometry, J. Mass Spectrom., 35(2), 131῍138(2000).

15)

T. Kashiwagi, N. Yamada, K. Hirayama,Hirayama C. Suzuki, Y. Kashiwagi, F. Tsuchiya, Y. Arata, N.
Kunishima, and K. Morikawa, An electrospray-ionization mass spectrometry analysis of thepH-dependent dissociation and denaturation processes of a heterodimeric protein, J. Am. Soc. MassSpectrom., 11(1), 54῍61 (2000).

῍ῌ1)   ῌ  !ῌ J. Mass Spec-trom. Soc. Jpn., 48(5), 289῍297 (2000).

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