J. Mass Spectrom. Soc. Jpn.

Vol. 55, No. 3, 2007

COMMENTARY
 
Optimization of Nano-Liquid Chromatography Coupled with
Mass Spectrometry Systems for Proteomics 1, 2
Yasushi IH=>=6B61, 2
1



Institute for Advanced Biosciences, Keio University, Tsuruoka,



PRESTO, Japan Science and Technology Agency, Kawaguchi,

YAMAGATA, JAPAN
2



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SAITAMA, JAPANCurrent proteomics technologies based on miniaturized on-line liquid chromatography (LC) coupled withmass spectrometry (MS) allow us to analyze protein expression proﬁling with a high throughput. Compared togenome sequencing and microarray-based transcriptomics, however, proteomics is still under development toachieve coverage of the entire proteome. In this commentary, the optimization of proteomic nanoLC/MS
systems is described. In addition, future development of the analytical systems to achieve the coverage of theentire proteome is discussed.
Received February 8, 2007; Accepted February 9, 2007

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Correspondence to: Yasushi IH=>=6B6, Institute forAdvanced Biosciences, Keio University, Nipponkoku 403῍1,
Daihouji, Tsuruoka, Yamagata 997῍0017, JAPAN, e-mail:y-ishi@ttck.keio.ac.jp
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Keywords: NanoLC-MS, Proteomics, LC fractionation, LC
optimization, Mass accuracy

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