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1High-Energy Collision-Induced Dissociation (CID)-MS/MS h<T {! V*R5SW)nV:,nO8-BX

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(2) MS/MS h LMsEM Sv¥¦$1RHH:;\q2 N-ev

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CID-MS/MS h< a-, b-e¦!%V~N:,nO8 n(:Su¬r:;+-e¦!% ~Nh+ H¯8-n'jv).
(3) 1  jskw°±^SWg) xy²w1B-¥³´µ¶'z2·{|J}B38 ¢1$ w7 GM1 jsku58 n w°±^VB~ {*+ V =?g' ESI-MS h<¸hu5E5M 1  o $¢171 : 1 5% ¹8 gn w°±^' GM1 º»jsk:;nOῌ 272 ῌ
(4)  '()*+,-./012324  !"#$%& FAB

 (Matrix-Assisted Laser Desorption/Ionization: MALDI) 56789:; <=> ?
@ABCDEF+G FAB CID-MS/MS EHIJK2LMNO PQ[1- C] HRDSTUVW X  <;NYZ[\G ESI-MS  ]=^13N;

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 <{v|+2G :N{v|^^YZ_G =^:; kG o ¡¢:;^ <£;¤¥_¦56§¨§¨©u } ª} =«¬N;

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C. Aaron, D. Dull, J. L. Schmiegel, D. Jaeger, Y. Ohashi,Ohashi and H. S. Mosher, The resolution andconﬁguration of a-substituted phenylacetic acids, J. Org. Chem., 32, 2797῍2803 (1967).
Y. Ohashi,Ohashi Structure determination of phospholipids by secondary ion mass spectrometric techniques: di#erentiation of isomeric esters, Biomedical Mass Spectrom., 11, 383῍385 (1984).
­G ®¯°±G FAB MS/SI MS ' bc5²³i6ῌ.EF´c5µ¶·¸i6
`¹ῌ¦§¨ºG 11, 1683῍1689 (1986).
Y. Ohashi,Ohashi M. Iwamori, T. Ogawa, and Y. Nagai, Analysis of long-chain bases in sphingolipids bypositive ion fast atom bombardment or matrix-assisted secondary ion mass spectrometry,Biochemistry, 26, 3990῍3995 (1987).
H. Hirota, A. Itoh, J. Ido, Y. Iwamoto, E. Goshima, T. Miki, K. Hasuda, and Y. Ohashi,Ohashi YS-822A, A
new polyene macrolide antibiotic, II. Planar structure of YS-822A, J. Antibiotics, 44, 181῍186(1991).
Y. Ohashi and Y. Nagai, Fast-atom-bombardment chemistry of sulfatide (3-sulfogalactosylceramide), Carbohydr. Res., 221, 235῍243 (1991).
T. OkinagaG Y. Ohashi,Ohashi and M. Hoshi, A novel saccharide structure, Xyl 1»3 Gal 1»(SO3ῌ)3,4 Fuc», is present in acrosome reaction-inducing substance of the starﬁsh, Asterias amurensis, Biochem.
Biophys. Res. Commun., 186, 405῍410 (1992).
T. Ii, Y. Ohashi,Ohashi and Y. Nagai, Observation of a novel adduct-ion in negative-ion electrospray mass
spectrometry of neutral glycolipids, Org. Mass Spectrom., 28, 927῍928 (1993).
T. Ii, Y. Ohashi,Ohashi Y. Matsuzaki, T. Ogawa, and Y. Nagai, Electrospray mass spectrometry ofpentacosasaccharides of blood group I-activity and related compounds, Org. Mass Spectrom., 28,1340῍1344 (1993).
S. Itonori, T. Shirai, Y. Kiso, Y. Ohashi,Ohashi K. Shiota, and T. Ogawa, Glycosphingolipid composition ofrat placenta: changes associated with stage of pregnancy, Biochem. J., 307, 399῍405 (1995).
T. Ii, Y. Ohashi,Ohashi and Y. Nagai, Structural elucidation of underivatized gangliosides by electrosprayionization tandem mass spectrometry (ESIMS/MS), Carbohydr. Res., 273, 27῍40 (1995).
T. IiG Y. OhashiG
Ohashi S. NunomuraG T. Ogawa, and Y. Nagai, Fast atom bombardment and electrosprayionization tandem mass spectrometry of sulfated Lewisx trisaccharides, J. Biochem., 118, 526῍533(1995).
M. Zhao, M. Yoneda, Y. Ohashi,Ohashi S. Kurono, H. Iwata, Y. Ohnuki, and K. Kimata, Evidence for thecovalent binding of SHAP, heavy chains of inter-a-trypsin inhibitor, to hyaluronan, J. Biol. Chem.,
270, 26657῍26663 (1995).
T. Shibue, H. Kambe, H. Nishide, and Y. Ohashi,
Ohashi Analysis of functional groups of porphyrins bycollision-induced dissociation mass spectrometry/mass spectrometry, Anal. Sci., 11, 793῍796(1995).
T. Ii, Y. Ohashi,Ohashi T. Ogawa, and Y. Nagai, Negative-ion fast atom bombardment and electrosprayionization tandem mass spectrometry for characterization of sulfated and sialyl Lewis-typeῌ 273 ῌ16)

17)

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21)

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23)

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glycosphingolipids, Glycoconjugate J., 13, 273῍283 (1996).
T. Ii, Y. Ohashi,
Ohashi T. Ogawa, and Y. Nagai, A new approach to the characterization of sulfated andsialyl Lewis-type glycosphingolipids using positive-ion FABMS, ESIMS, and CID-MS/MS, J. Mass
Spectrom. Soc. Jpn., 44, 183῍195 (1996).
T. Shibue, H. Kambe, H. Nishide, S. Akashi, N. Dohmae, and Y. Ohashi,Ohashi Structural characterizationof “tailed picket-fence porphyrins” by high-energy fast atom bombardment collision-induced
dissociation mass spectrometry/mass spectrometry, Eur. Mass Spectrom., 3, 291῍299 (1997).
T. Shibue, H. Kambe, H. Nishide, and Y. Ohashi,Ohashi Comparison of electron ionization and fast atom
bombardment ionization for CID-MS/MS studies of “Picket-Fence” porphyrin, Anal. Sci., 13, 845῍847 (1997).
Y. Ohashi,Ohashi T. Ii, M. Kubota, S. Nunomura, H. Niwa, M. Ohashi, T. Ogawa, and Y. Nagai, FAB
CID-MS/MS of in-source fragment ions as a means to di#erentiate linkage isomers of branchedoligosaccharides: Lea and Lex type glycoconjugates, J. Mass Spectrum. Soc. Jpn., 46, 45῍52 (1998).
T. Shibue, H. Kambe, H. Nishide, E. Tsuchida, S. Akashi, and Y. Ohashi,Ohashi Structural characterizationof picket-fence porphyrinatoiron with w-hydroxy-2,2-dimethylicosanoyl side-chains vs. wcholinephosphoryl-2,2-dimethylicosanoyl side-chains by FAB high-energy-CID MS/MS, J. Mass
Spectrom. Soc. Jpn., 46, 250῍254 (1998).
S. Kurono, Y. Ohashi,Ohashi K. Hiruma, T. Okinaga, M. Hoshi, H. Hashimoto, and Y. Nagai, Characterization of the sulfated fucose-containing trisaccharides by fast atom bombardment tandem mass
spectrometry in the study of the acrosome reaction-inducing substance of the starﬁsh, Asterias
amurensis, J. Mass Spectrom., 33, 35῍44 (1998).
Y. Ohashi,Ohashi T. Tanaka, S. Akashi, S. Morimoto, Y. Kishimoto, and Y. Nagai, Squid nerve sphingomyelin containing an unusual sphingoid base, J. Lipid Res., 41, 1118῍1124 (2000).
Y. Itoh, Y. Ohashi,Ohashi T. Shibue, A. Hayashi, S. Maki, T. Hirano, and H. Niwa, Reduction in desorption
mass spectrometry: multiple protonation on ﬂavins without charge increment, J. Mass Spectrom.
Soc. Jpn., 50, 52῍57 (2002).
Y. Ohashi and Y. Itoh, Unprecedented matrix-induced reduction of ﬂavins observed under FABand MALDI conditions, Current Org. Chem., 7, 1605῍1611 (2003).
Y. Ohashi,Ohashi Y. Itoh, M. Kubota, K. Hamada, M. Ohashi, T. Hirano, and H. Niwa, Analysis of sugarepimers using mass spectrometry: N-acetyllactosamine-6,6῎-disulfate and the 2῎-epimer, Eur. J.
Mass Spectrom., 10, 269῍278 (2004).
Y. Ohashi,Ohashi H. Hatase, M. Nakamura, T. Hirano, S. Maki, and H. Niwa, Characterization of [1-13C]speciﬁcally labeled amino acids using ESIMS/MS in the selected reaction monitoring mode, J.
Mass Spectrom. Soc. Jpn., 53, 309῍314 (2005).

ῒῑῌ῎῍῏ῑ1)   I 
 21, 483῍490 (1968).
2)   II 
 21, 630῍637 (1968).
3)    !"#$% &'"()*+,-  59, 1138῍1143 (1987).
4) Y. Ohashi,
Ohashi How to obtain molecular weight information on large moleculesῌMultiply charged
ions on electrospray ionization mass spectrometry, Trends in Glycoscience and Glycotechnology(TIGGTM), 2, 45῍49 (1990).
5)   ./0 Chemistry ’90,  45, 730῍731 (1990).
12% &'"()*6)   "()*% &'3456+ 789:;<=8>?@A +
 46, 627῍633 (1991).
7)   MS %BCDEFGH' IJKLMNOP'QR
ST 122, 143῍153 (1992).
8)   UVW"()*+%'XYC"EFZ* Y[\]^ 51, 529῍540 (1993).
9) Y. Ohashi and Y. Nagai, Electrospray ionization mass spectrometry: Using biopolymers to extend
the concept of heavy ions, RIKEN Review, 4, 59῍60 (1994).
10)  _`a CDEFZ*bc>d9e;f>῍3 g"()*+h! 32,741῍750 (1994).
11) Y. Ohashi,Ohashi S. Kurono, and Y. Nagai, Adventures in glyco-mass-spectrometry: Seeking for a bettermethod, RIKEN Review, 8, 45῍46 (1995).
12)   789:;<=8>?@A "()*+  67, 248 (1995).
13)   
 67, 1295῍1301 (1995).
12% &'ij"()* ΐῐ1) klmn o pq   ./0 rs
tu`a v 4 w XYC"#$+ II. Cx"=;d@yz{A| }~]   (1986), pp. 157῍180.
2)     rtu  2ῌEFZ*ῌ| n  n ῌ 274 ῌ3)
4)
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8)

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  (1989),  3  pp. 180῍197.
   17    (1990).
  ! 4 " #$ ῌῌ%#$ῌ &'()  (1990), pp. 180῍197.
Y. Ohashi,Ohashi D. A. Gage, and C. C. Sweeley, Gangliosides and Neutral Glycosphingolipids, “Techniques in Diagnostic Human Biochemical Genetics: A Laboratory Manual,” ed. by F. Hommes,
Wiley-Liss Inc., New York (1991), pp. 239῍265.
 *+-. /0$123456789%$0:;<= %>? %>?)*+ ,@AB() CDEF(GHIJKLMNOPQRSTO  (1992)  V  2, 434῍451.
 2 UVW XYZ [)   (1997).
Y. Ohashi,Ohashi Electrospray Ionization Mass Spectrometry of Carbohydrates and Lipids, “ElectrosprayIonization Mass Spectrometry: Fundamentals, Instrumentation, and Applications,” ed. by R. B.
Cole, John Wiley & Sons, Inc., New York (1997), Chap. 13, pp. 459῍498.
Y. Ohashi,Ohashi S. Kurono, and Y. Nagai, Analysis of Carbohydrate῍Protein Interactions by Mass
Spectrometry, “Techniques in Glycobiology,” ed. by R. R. Townsend and A. T. Hotchkiss, Jr.,Marcel-Dekker, Inc., New York (1997), pp. 77῍83.
   18    (1998).

ῌ 275 ῌ