Health food ingredients with good safety and functionality of years of research

Agaricus Blazei Murill Extract-H
What is Agaricus Blazei Murill?
Agaricus Blazei Murill is a mushroom of Agaricaceae and is said to originate in theBrazilian Piedade Mountains of South America.

Functional components of Agaricus mushrooms (polysaccharide protein)
There is a tradition saying that it is a "healthy mushroom". In 1980s, the research on the functionality of Agaricus Blazei
Murill began, and it is the mushroom which the research was actively promoted in Japan. Also, in the United States,
clinical trials have been conducted at the National Cancer Institute and many academic reports have been published.
The studies of Agaricus Blazei Murill in animal studies are known for activation of immune cells such as hostmacrophages, complement and other immune cells, as well as the activation of interferons，the promotion effect ofimmune system such as cytokine induction. The active ingredients in mushroom are known as lentinan from shiitake
mushrooms, crestin from Kawaratake mycelium, schizophyllan from Suehiro mushroom and neutral or acidpolysaccharides mainly consisting of β (1-3)-D-or β-(1-6)-D-glucan.
These β-glucans are compounds which are widely distributed as cell wall components of mushrooms.
The high functionality of Agaricus mushrooms taken by oral ingestion cannot be explained solely by β-glucan. In the
study of Mizuno, a macromolecular polysaccharide protein complex containing β-glucan which is called as AB-P isintroduced as a functional component of Agaricus.
Literature: Mizuno S, The Chemical Times, 1, 12-21 (1989)

Immunostimulatory effectResistance to infections
Mice were fed Agaricus mushroom extract or Agaricus
mushroom-derived polysaccharide protein complexes for 14 days
and then infected with Pseudomonas aeruginosa 2×107 cfu)
intraperitoneally to observe viability.
All non-ingested controls died 3 days post-infection
However, 1 of 8 mice survived in the Agaricus mushroom extractgroup and 3 mice survived in the polysaccharide protein complex
group.

Number of surviving animals

The immune system essential for health is reduced by external and internal stresses and fatigue, which predisposes toillnesses such as cold. It is said that the immunity decreases with age. The polysaccharide protein complex in Agaricus
Blazei extract has been shown to activate immune vesicles to enhance immune competence.
control
Agaricus Extract
Polysaccharide protein

Days

Antiviral resistanceLow-dose
infection
Survival rate

Mice were infected with influenza A virus (H l N l) to a
non-pathogenic extent and then infected with a stronger virus by
tail-vein injection,
30% of the mice died. On the other hand, mice fed with Agaricus
mushroom extract for 14 days showed 100% survival in a same
study, and the virus
infection resistance was confirmed.

Massive
infection

Agaricus dosing group
Salt water dosing groupDays

Safety and efficacy of Agaricus Blazei extract in clinical trials
At the 12th Japan Society for Complementary and Alternative Medicine

No abnormalities were observed in the blood testsof those who consumed Agaricus -processed foods,
confirming that the safety was high. In addition, the activity ofNK cells, which plays an important role in the immune system,
was activated by the consumption of Agaricus -processedfoods.On the other hand, CRP (C-reactive protein) indicating
an inflammatory state in vivo was also improved by theconsumption of Agaricus -processed foods.
In QOL questionnaires, patients who consumed Agaricus-processed foods showed improvements in physical pain,
general feelings of wellbeing, and vitality.
ResultAgaricus processed foodintake period:

control
1pack/dayserum

2nd week 4th week

3pack/dayNumber

4th week

NK cell activity was significantly elevated byconsumption of 3 pack/day. Intake-dependent
activation was observed◎ IEfficacy in improving QOL

3pack/dayNumbercontrol
1pack/day

Post
inspection

Compared to the control group, both 1 pack/day and 3pack/day consumption showed a decrease in CRP andremained low even 1 week after the end of the test.

Changes in standardized scores
before and after ingestion◎ Reduced in vivo inflammation◎ Immunostimulatory effectNumber of surviving animals

Forty-five subjects, including obese and smokers,
were randomly divided into three groups: no intake group(control), 1 pack/day intake of Agaricus Blazei extract
processed food, and 3 pack/day intake group (n=15). Bloodtests and a questionnaire survey were performed after
consuming them for 28 days.
Test

everyday role
functioning

Social function
General health
Vitality
perception

Body pain
control

1pack/day

3pack/day

According to the questionnaire after intaking Agaricus,
improvement was observed in various items for measuring
QOL. In particular, significant improvements in physical pain,
overall health and vitality were observed.

Product specification
Product information
Items
Appearance
Moisture content
Polysaccharide
protein complex
Heavy metals (Pb)
Arsenic (As 2 Q 3)
Total plate countColiforms
Daily dosage

Specification
Reddish brown to darkbrown powder
max. 8.0%
min.10.0%
max. 20 ppm
max. 2 ppm
max.3000cfu/gNegative
1,350~ 2,700 mg/day

Allergy indication: no need
Genetically modified: noRaw material of animal derived : noLabel indication: Agaricus Blazei Extract◆Packing: 1kg 〜◆Type of packing: Depending on the content ( PE bag/cardboard )◆Storage: please keep it in the place where is cool and dry.

◆Expiry date: 3 years after production（condition: unopened state）◆Quality control: each lot[ Headquarters ] 1-16, Kandanishiki-cho, Chiyoda-ku, Tokyo

www.bhn.co.jp

TEL:+81-3-5281-5661

FAX:+81-3-5281-5662