2009A1851                             BL40XUࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺ࡜ࣜࣥ㓟໬࢜ࣜࢦ⢾࢝ࣝࢩ࣒࢘࡟ࡼࡿ෌⤖ᬗ໬
Recrystallization Effect by POs-Ca and Nanoparticle Hydroxyapatite⏣୰ ᬛᏊ a, ▼ሯ ⣪࿴Ꮚ a, ⁪஭ ᐶ a, 㔩㜰 ᐶ a,ኴ⏣ ᪼ b, ᯇᑿ 㱟ே b, ඵᮌ ┤ே b, ⡿㇂ ಇ a
Tomoko Tanakaa, Sawako Ishiduka a, Hiroshi Takii a, Hiroshi Kamasaka a,
Noboru Ohta b, Tatsuhito Matsuo b, Naoto Yagi b, Takashi Kometani aaỤᓮࢢࣜࢥ㻔ᰴ㻕೺ᗣ⛉Ꮫ◊✲ᡤ, b㻔㈈㻕㧗㍤ᗘග⛉Ꮫ◊✲ࢭࣥࢱ࣮aHealth Science Laboratory, Ezaki Glico Co.,Ltd,䢢bThe Japan Synchrotron Radiation Research Instituteᚑ᮶ࠊࣜࣥ㓟໬࢜ࣜࢦ⢾࢝ࣝࢩ࣒࢘㻔POs-Ca㻕࡟ࡣึᮇ࠺⼃ࡢ෌▼⅊໬ຠᯝࡀ▱ࡽࢀ࡚࠸ࡿࠋࡲࡓ᭱㏆ࡢ◊✲࠿ࡽࠊࡑࡢ෌▼⅊໬㒊఩ࡣࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ࡜ࡋ࡚⤖ᬗ໬ࡋ࡚࠸ࡿࡇ࡜ࢆࠊX ⥺࣐࢖ࢡࣟࣅ࣮࣒ࢆ⏝࠸࡚㧗ゅ X ⥺ᅇᢡゎᯒ࡛☜ㄆࡉࢀࡓࠋᮏ◊✲࡛ࡣ POs-Ca ࡟ࢼࣀ⢏Ꮚ໬ࣁ࢖ࢻࣟ࢟ࢩ
࢔ࣃࢱ࢖ࢺࢆຍ࠼ࡿࡇ࡜࡛ึᮇ࠺⼃ࢆฎ⌮ࡋࡓሙྜࠊᚑ᮶ࡢຠᯝࡼࡾ㧗࠸෌⤖ᬗ໬ࡀᮃࡵࡿ࠿࡝࠺࠿ࡢ᳨ウࢆ⾜ࡗࡓࠋ⤖ᯝࠊPOs-Ca ༢⊂ࡼࡾࡶඃࢀࡓ෌⤖ᬗ໬ຠᯝࡀほࡽࢀࡓࠋ࣮࣮࢟࣡ࢻ㸸  ᗈゅ X ⥺ᅇᢡࠊࣜࣥ㓟໬࢜ࣜࢦ⢾࢝ࣝࢩ࣒࢘ࠊࢼࣀ⢏Ꮚ໬ࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࠊึᮇ࠺⼃⫼ᬒ࡜◊✲┠ⓗ㸸Ụᓮࢢࣜࢥ㻔ᰴ㻕࡛ࡣࠊ㤿㕥⸉⃦⢊࠿ࡽࣜࣥ㓟ᇶࢆ᭷ࡍࡿ᪂ࡋ࠸࢜ࣜࢦ⢾㸹ࣜࣥ㓟໬࢜ࣜࢦ⢾࢝ࣝࢩ
࣒࢘㻔POs-Ca㻕ࡢㄪ〇࡟ᡂຌࡋࡓ[1]ࠋ⢾໬⏘ᴗ࡟࠾࠸࡚ࠊࣜࣥ㓟໬࢜ࣜࢦ⢾ࡣᮍ฼⏝⏬ศ࡜ࡋ࡚ᗫᲠࡉ
ࢀ࡚ࡁ࡚࠸ࡓࡇ࡜࠿ࡽࠊPOs-Ca ࡣࣂ࢖࣐࢜ࢫࡢ᭷ຠ฼⏝࡜࠸࠺ഃ㠃ࢆ᭷ࡋ࡚࠸ࡿࠋࡇࡢ POs-Ca ࡢຠᯝࡣࠊ࢝ࣝࢩ࣒࢘࢖࢜ࣥࢆ࢜ࣜࢦ⢾ࡀᣢࡘࣜࣥ㓟ᇶ࡛Ᏻᐃ໬ࡍࡿࡇ࡜࡛ࠊ௚ࡢ࢝ࣝࢩ࣒࢘⣲ᮦ࡟ẚ࡭࡚㧗࠸࢝ࣝࢩ࣒࢘ࡢỈ⁐ᛶࢆᐇ⌧ࡋ࡚࠸ࡿࠋࡑࢀࡺ࠼ࠊPOs-Ca ࡣၚᾮ࡬ࡶ㏿ࡸ࠿࡟⁐ゎࡋ࡚Ỉ⁐ᛶ࢝ࣝࢩ࣒࢘ࢆቑຍࡉࡏࡿࡇ࡜ࡀ࡛ࡁࡿࠋࡇࢀࡲ࡛ࠊPOs-Ca ࡟ࡘ࠸࡚ࡣึᮇ࠺⼃㻔࢚ࢼ࣓ࣝ㉁⾲㠃࡟ᐇ㉁ⓗḞᦆࡀ࡞ࡃࠊ⾲ᒙୗ࠿ࡽ࣑ࢿࣛࣝࡀ⁐ฟࡋ࡚࣑ࢿࣛࣝ⃰ᗘࡀ␯࡟࡞ࡗ࡚࠸ࡿ≧ែ㻕ࡢ෌▼⅊໬ຠᯝࡀ㧗࠸ࡇ࡜ࢆᅜ㝿ⓗ࡞ᶆ‽ゎᯒἲࡢ୍ࡘ࡛࠶ࡿ Transverse microradiography 㻔TMR㻕ἲࢆ⏝࠸࡚ᐇドࡋ࡚ࡁࡓ[2][3]ࠋࡋ࠿ࡋ TMR ἲ࡛ࡣึᮇ࠺⼃ࡢ㉳ࡇࡿ࢚ࢼ࣓ࣝ㉁㒊ศࡢ࣑ࢿࣛࣝࡢྵ᭷ኚ໬⋡࡜ࡋ࡚ࡋ࠿ᤊ࠼ࡿࡇ࡜ࡀ࡛ࡁ࡞࠸ࡓࡵࠊᅇ᚟ࡋࡓ࣑ࢿࣛࣝࡀࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࡢ⤖ᬗ࡜ࡋ࡚࡝ࡢࡼ࠺࡟ᅇ᚟ࡋ࡚࠸ࡿ࠿ᐃ㔞ⓗ࠿ࡘᐃᛶⓗ࡟ᤊ࠼ࡿࡇ࡜ࡀ࡛ࡁ࡞࠿ࡗࡓࠋᮏຠᯝࢆ᫂࠿࡟ࡍࡿࡇ࡜ࡣᅜẸࡢṑࡢ೺ᗣ⥔ᣢቑ㐍࡟㈉⊩࡛ࡁࡿᇶ┙ᢏ⾡㛤Ⓨ࡟⧅ࡀࡿࡶࡢ࡛࠶ࡿࠋࡑࡇ࡛ࠊ
ᡃࠎࡣ X ⥺ᅇᢡἲࢆ⏝࠸࡚ࠊ
TMR ᐃ࡛⏝࠸ࡓேᕤⓗ࡟⬺⅊≧ែࢆసࡾฟࡋࡓṑ∦㻔150 ȝmཌ㻕ࡢ࢚ࢼ࣓ࣝ㉁୰ࡢᚤᑠ㡿ᇦࡢࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ⤖ᬗኚ໬ࢆᐃ㔞໬ࡍࡿࡇ࡜ࢆヨࡳࡓࠋ⾲ᒙୗ⣙ 200 ȝm ࡢ⠊ᅖ࡛ࡢ⤖ᬗኚ໬ࢆ⣽࠿ࡃᤊ࠼ࡽࢀࡿࡼ࠺࡞ X ⥺ࢆ⏝࠸ࡓゎᯒ᪉ἲࡣࠊṑ⛉࡟࠾࠸࡚ࡣࡇࢀࡲ࡛౛ࡀ࡞࠿ࡗࡓࠋ㧗ศゎ⬟࡛⤖ᬗ໬㔞ࡢࣉࣟࣇ࢓࢖ࣝࢆ⟬ฟࡍࡿࡓࡵ࡟ࡣࠊ▷᫬㛫࡛ 1 ༊⏬ࡈ࡜ࡢ ᐃࢆ⾜࠺ᚲせࡀ࠶ࡿࡓࡵࠊSPring-8 ࡢ㧗㍤ᗘ࡛ᴟᑠ࡟⤠ࡾ㎸ࢇࡔ࣐࢖ࢡࣟࣅ࣮࣒ࡀᚲせ࡛࠶ࡗࡓࠋࡑࡇ࡛ 2007 ᖺᗘࡢ SPring-8 ࡢㄢ㢟࡟࠾࠸࡚ࠊ6 ȝm ࡟⤠ࡗࡓ࣐࢖ࢡࣟࣅ࣮࣒ࢆ⏝࠸ࡓᗈゅ X ⥺ᅇᢡἲ࠾ࡼࡧᑠゅ X ⥺ᩓ஘ἲࢆྠ᫬ ᐃ࡛ࡁࡿ BL40XU ࢆ⏝࠸࡚ゎᯒࡍࡿ᪉ἲࢆ☜❧ࡋ[4]ࠊࡑࡢ⤖ᯝṑ∦ࡢ෌▼⅊໬㒊ศࡀࠊ೺ᗣ࡞ṑ࡜ྠᵝࡢࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ⤖ᬗ࡜ࡋ࡚᚟ඖࡋ࡚࠸ࡿࡇ࡜ࢆึ
ࡵ࡚᫂ࡽ࠿࡟ࡋࡓ[5]ࠋࡲࡓࠊᑠゅᩓ஘ἲ࡟ࡼࡾࠊ⬺⅊ᝈ㒊ࡣࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ⤖ᬗ༢఩࡛ࢼࣀࢧ࢖ࢬࡢ✵㝽ࡀ⏕ࡌ࡚࠸ࡿࡇ࡜ࡶゎࡗࡓ[5]ࠋᮏ◊✲ㄢ㢟࡛ࡣࠊ᪂ࡓ࡟ᚑ᮶ࡼࡾࡶඃࢀࡓ෌▼⅊໬ࢆ♧ࡋࡓ POs-Ca ࡜ࢼࣀ⢏Ꮚ໬ࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࢆྵࡴ⤌ᡂࡢ⁐ᾮࡢฎ⌮࡟ࡼࡾࠊ෌⤖ᬗ໬ࡀ࡝ࢀࡔࡅಁ㐍ࡉࢀ࡚࠸ࡿ࠿ࢆ X ⥺ᅇᢡἲ࡟ࡼࡾ᫂ࡽ࠿࡜ࡍࡿࡇ࡜ࢆ┠ᣦࡋࡓࠋᐇ㦂㸸㹙ヨᩱ㹛࢘ࢩṑ࢚ࢼ࣓ࣝ㉁ࢫࣛ࢖ࢫᶆᮏࢆヨ㦂ヨᩱ࡜ࡋࡓࠋᮏヨᩱࡣ∵ᾏ⥥≧⬻⑕ᑐ⟇≉ูᥐ⨨ἲ࡟ᇶ࡙ࡁ኱㜰ᕷ㣗⫗⾨⏕᳨ᰝᡤෆ᳨࡛ᰝࡉࢀࠊチྍࡉࢀศㆡࢆཷࡅࡓ࢘ࢩṑ࠿ࡽㄪ〇ࡋࡓࠋヨᩱࡣ࢘ࢩṑෙ㒊ࡼࡾࣈࣟࢵࢡ≧࡟ษࡾฟࡋ㻔⣙ 10mm10mm×2mm㻕ࠊࣞࢪࣥᶞ⬡࡟࢚ࢼ࣓ࣝ㉁㠃௨እࢆໟᇙࡋࠊ᪂ࡋ
࠸࢚ࢼ࣓ࣝ㉁㠃ࢆ◊☻ฎ⌮࡛㟢ฟࡉࡏࡓࠋ࢚ࢼ࣓ࣝ㉁⾲㠃⣙ 1/3 ࢆ೺඲㠃࡜ࡋ࡚ࢿ࢖ࣝࣂ࣮ࢪࢵࢩ࡛ࣗ⿕そࡋࡓ೺඲㒊఩㻔SOUND㻕ᚋࠊ⬺⅊ฎ⌮ࢆ⾜ࡗࡓࠋ⬺⅊ฎ⌮ᚋࠊ⬺⅊⾲㠃⣙ 1/2 ࢆ⿕そࡋ࡚⬺⅊㒊఩㻔DEM㻕࡜ࡋࠊṧࡾ 1/2 㠃࡛෌▼⅊໬㒊㻔REM㻕ࢆ⾜ࡗࡓࠋ෌▼⅊໬ฎ⌮ᚋࠊᶓ᩿㠃ࢆษࡾฟࡋࡓࢫࣛ࢖
ࢫᶆᮏ㻔150ȝm×10mm×5mm㻕ࢆヨ㦂ヨᩱ࡜ࡋࡓࠋ
෌▼⅊໬ฎ⌮ࡢ᪉ἲࢆḟ࡟♧ࡍࠋ⾲ᒙୗ⬺⅊⑓ᕢࢆᙧᡂࡉࡏࡓ࢚ࢼ࣓ࣝ㉁ࣈࣟࢵࢡࢧࣥࣉࣝࢆ෌▼⅊໬⁐ᾮ࡟ 5 ṑ∦ࡎࡘ 72 ᫬㛫㸪 37Υ࡛ᾐₕฎ⌮ࢆ᪋ࡋࡓࠋ෌▼⅊໬⁐ᾮࡣࠊ20 mM HEPES ⦆⾪ᾮࠊ
6 mM ࢝ࣝࢩ࣒࢘ࠊ3.6 mM ࣜࣥ㓟ࠊ100 mM KCl ࡟ࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺࢆῧຍࡋࠊ1N KOH ⁐ᾮ࡛ pH 6.5 ࡟ㄪᩚࡋࡓࠋ࢝ࣝࢩ࣒࢘※࡜ࡋ࡚ࠊPOs-Caࠊࣜࣥ㓟※࡜ࡋ࡚ KH2PO4 ࢆ⏝࠸ࡓ㻔⾲ 1㻕ࠋ㹙᪉ἲ㹛࠙Transverse microradiography㻔TMR㻕࣑ࢿࣛࣝᅇ᚟㔞ࡢゎᯒࠚ෌▼⅊໬ฎ⌮ᚋࡢ࢚ࢼ࣓ࣝ㉁ࣈࣟࢵࢡヨ㦂㠃୰ኸࡼࡾ SOUND/DEM/REM ࡢ 3 㡿ᇦ㠃඲࡚ࡀྵࡲࢀࡿࡼ࠺࡟ཌࡉ⣙ 150 ȝm ࡢษ∦ࢆྛヨᩱࣈࣟࢵࢡ࠿ࡽษࡾฟࡋࠊTMR ἲࡼࡾᚓࡽࢀࡓ࣐࢖ࢡࣟࣛࢪ࢜ࢢࣛࣇࡼࡾࠊ࢚ࢼ࣓ࣝ⾲ᒙ࠿ࡽ㇟∳㉁࡟࠿ࡅ࡚ࡢ࣑ࢿࣛࣝศᕸ㻔࣑ࢿࣛࣝࣉࣟࣇ࢓࢖ࣝ㻕࠿ࡽ࣑ࢿࣛࣝ႙ኻ㔞㻔ԥZ㻕ࢆ ᐃࡋࡓࠋ෌▼⅊໬⋡㻔ԥZ DEM -ԥZ REM㻕/ ԥZ DEM 㻔࡞࠾ԥZ DEM ࡣ DEM 㠃࡟࠾ࡅࡿ࣑ࢿࣛࣝ႙ኻ㔞ࠊԥZ REM ࡣ REM 㠃࡟࠾ࡅࡿ࣑ࢿࣛࣝ႙ኻ㔞ࢆ♧ࡍ㻕ࢆ⟬ฟࡋࠊ෌▼⅊໬ຠᯝࡢᣦᶆ࡜ࡋࡓࠋ
࠙ᗈゅ X ⥺ᅇᢡࠚ
BL40XU ࡢ X ⥺ࢆࠊࣆ࣮ࣥ࣍ࣝࢆ㏻ࡋ࡚┤ᚄ⣙ 6 ȝm ࡢ࣐࢖ࢡࣟࣅ࣮࣒࡜ࡋࡓࠋ࣓࢝ࣛ㛗ࡣ 3000 mm⛬ᗘ࡜ࡋࡓࠋᗈゅᩓ஘ࡣࠊࣇࣛࢵࢺࣃࢿ᳨ࣝฟჾ㻔C9728DK, ὾ᯇ࣍ࢺࢽࢡࢫ㻕ࢆ┿✵ࣃ࢖ࣉࡢ๓㻔ヨᩱ࠿ࡽ 20 cm ⛬ᗘࡢ఩⨨㻕࡟⨨࠸࡚グ㘓ࡋࡓࠋࢫࣛ࢖ࢫ≧ࡢヨᩱࢆ X ⥺ࡀᆶ┤࡟ධᑕࡍࡿࡼ࠺࡟ࢭࢵࢺࡋ㸪X ⥺ࣅ࣮࣒ࡀ࢚ࢼ࣓ࣝ㉁ࡢୖ➃࠿ࡽṑࡢෆ㒊࡟ධࡿ᪉ྥ࡟㸪ヨᩱࢆ 5ȝm ࢫࢸࢵࣉ࡛⛣ືࡋࠊ㏲ḟ
ᅇᢡࢹ࣮ࢱࢆ཰㞟ࡋࡓ[4]ࠋࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࡢ㻔100㻕㠃⏤᮶ࡢ཯ᑕ࡟┦ᙜࡍࡿ X ⥺ᅇᢡᩬࡢᙉᗘ✚⟬࠿ࡽࠊࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ⤖ᬗ㔞ࢆᐃ㔞ࡋࡓࠋࡉࡽ࡟ࠊࣉࣟࣇ࢓࢖ࣝ࡟ࢃࡓࡗ࡚⾲ᒙ࠿ࡽ 200ȝm ࡲ࡛ࡢࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ㔞ࢆ✚ศ࡟ࡼࡾồࡵࠊ⤖ᬗ๭ྜ㔞 ᐃ್㻔೺඲㒊㸹sࠊ⬺⅊㒊㸹dࠊ෌▼⅊໬㒊㸹r㻕࡜ࡋࡓࠋ෌⤖ᬗ໬⋡ࡣ㻔s㸫d㻕㻛㻔r㸫d㻕×100 ࡢィ⟬್ࡼࡾ⟬ฟࡋࡓࠋ⤖ᯝ࠾ࡼࡧ⪃ᐹ㸸
POs-Ca ࡜ࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺ࡛෌▼⅊໬ฎ⌮ࢆ᪋ࡍ࡜ࠊPOs-Ca ༢⊂ࡢࡼࡾࡶඃࢀࡓ෌▼⅊໬࡜෌⤖ᬗ໬ຠᯝࡢ୧᪉ࡢຠᯝࡀ࠶ࡿࡇ࡜ࡀ☜ㄆࡉࢀࡓ㻔ᅗ 1㻕ࠋࡋ࠿ࡋࠊࡑࡢᗘྜ࠸ࡣ෌▼⅊໬ຠᯝࢆ♧ࡍ
TMR ἲ࡜෌⤖ᬗ໬ຠᯝࢆ♧ࡍᗈゅ X ⥺ᅇᢡἲ࡛ࡣ␗࡞ࡿഴྥࢆ♧ࡋࡓ㻔ᅗ 2㻕ࠋࡘࡲࡾࠊᗈゅ X ⥺ᩓ஘ἲ࡛ࡣࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺࡢ⢏Ꮚࢧ࢖ࢬࡀᑠࡉ࠸࡯࡝ຠᯝⓗ࡛࠶ࡿࡀࠊTMR ἲ࡛ࡣྠࡌ⤖ᯝ࡟ࡣ࡞ࡽ࡞࠿ࡗࡓࠋࡇࡢࡇ࡜ࡣ TMR ἲ࡛ࡣࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࡢ⤖ᬗ≀௨እࡢ࣑ࢿࣛࣝሁ✚≀ࡀ᳨ฟࡉࢀ࡚࠸ࡿࡶࡢ࡜⪃࠼ࡽࢀࡿࠋ௒ᚋࡢㄢ㢟㸸ࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺ࡜ POs-Ca ࡢే⏝࡟ࡼࡗ࡚ࠊඃࢀࡓ෌⤖ᬗ໬ຠᯝࡶぢฟࡏࡓࠋ௒ᚋࠊTMR ἲ
࡜ᗈゅ X ⥺ᅇᢡἲ࡛ࡢ⢏Ꮚᚄ࡛ࡢຠᯝࡢ㐪࠸࡟ࡘ࠸࡚෌⌧ᛶࢆྵࡵ࡚ヲ⣽࡟᳨ウࡋࠊ⬺⅊ᗘ࡜⢏Ꮚᚄࡢ┦㛵ࢆྵࡵ᳨࡚ウࢆ㐍ࡵࡓ࠸ࠋࡲࡓࠊ㟁Ꮚ㢧ᚤ㙾࡛ࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺࡀ࡝ࡢࡼ࠺࡟ྲྀࡾ㎸ࡲࢀ࡚࠸ࡿ࠿࡞࡝ࢆほᐹ࡟ࡼࡾ᳨ウࢆ㐍ࡵࡓ࠸ࠋࡲࡓ⾲ᒙୗ⬺⅊ࡣࠊ࢚ࢼ࣓ࣝ⾲ᒙ࠿ࡽ 20 ȝm ࡛ᛴ⃭࡟࣑ࢿࣛࣝ㔞ࡀపୗࡍࡿ⌧㇟࡛࠶ࡿࠋ⬺⅊ࡀୗᒙ࡛㉳ࡇࡗ࡚࠸ࡿ࠿ࠊ࠶ࡿ࠸ࡣୖᒙ࡛㉳ࡇࡗ࡚࠸ࡿ࠿ࡣᏛ⾡ⓗ࡟ࡶၟရ㛤Ⓨࡢୖ࡛ࡶ㔜せ࡞ㄢ㢟࡛࠶ࡿࠋࡑࡢ⌧㇟ࢆཝᐦ࡟ゎᯒࡍࡿ࡟ࡣࠊ6 ȝm ࡜࠸࠺ࣅ࣮࣒┤ᚄ࡛Ỵࡵࡽࢀࡿศゎ⬟࡛ࡣࠊࡲࡔ༑ศ࡜ࡣ࠸࠼࡞࠸ࠋ௒ᚋࡣࡉࡽ࡞ࡿ࣐࢖ࢡࣟࣅ࣮࣒ࡢᛶ⬟ࢆྥୖࡉࡏࡽࢀࡿྍ⬟ᛶࡀ࠶ࡿ࠿࡝࠺࠿ࢆ᳨ウࡍࡿ࡜࡜ࡶ࡟ࠊ༢⣧࡞㉮ᰝ࡛ࡣ࡞ࡃࠊ ᐃἲࡸゎᯒἲࢆᕤኵࡍࡿࡇ࡜࡟ࡼࡿศゎ⬟ࡢྥୖࡀ࡛ࡁࡿ࠿࡝࠺࠿ࢆ᳨ウࡋ࡚࠸ࡁࡓ࠸࡜⪃࠼࡚࠸ࡿࠋࡲࡓࠊᮏ◊✲࡟࠾࠸࡚ࡣࠊ⏕≀ࡢṑ࠿ࡽṑ∦ࢆㄪ〇ࡋࠊࡑࢀࢆヨᩱ࡜ࡋ࡚⏝࠸࡚࠸ࡿࠋࡑࢀࡒࢀࡢṑ࡟ࡘ࠸࡚ࠊ࢚ࢼ࣓ࣝ㉁ࡢ㐪࠸ࡀ࠶ࡿୖ࡟ࠊヨᩱㄪ〇࡟࠾࠸࡚ࠊࡉࡽ࡟ヨᩱࡈ࡜ࡢࡤࡽࡘࡁࡀ⏕ࡌࡸࡍ࠸ࠋᚑࡗ࡚ࠊከࡃࡢヨᩱࢆ ᐃࡋࠊᚓࡽࢀࡓࢹ࣮ࢱࢆ⤫ィฎ⌮ࡋ࡞ࡅࢀࡤࠊ᭷ព࡞⤖ᯝࢆᚓࡿࡇ࡜ࡀ࡛ࡁ࡞࠸ࠋࡑࡇ࡛ࠊ௒ᚋࡣࡼࡾከࢧࣥࣉࣝࢆ㎿㏿࡟ ᐃ࡛ࡁࡿࡼ࠺࡞ヨᩱ࣐࢘ࣥࢺࡢ᪉ἲࡸ㉮ᰝࡢ᪉ἲࠊࡲࡓ᳨ฟࡸゎᯒࡢ⮬ື໬࡞࡝ࡀㄢ㢟࡜࡞ࡿ࡜⪃࠼ࡽࢀࡿࠋ⾲ 1෌▼⅊໬⁐ᾮ⤌ᡂ⤌ᡂ⃰ᗘῧຍ㔞㻔ml㻕
Distilled Water
50
KCl
2M
5HCl
1M
0.05
KH2PO4100mM
3.6 㻔1.5㻕 ***nHAp or HAp*
0.1
10%POs-Ca**
4.8
CaCl2**
100mM
㻔0.9㻕 ***
HEPES
200mM
10KOH
1M
pH 6.5 ࡟ㄪᩚTotal100㹫㹊*ྛ✀ࢧ࢖ࢬ࡟ຍᕤࡉࢀࡓࢼࣀ⢏Ꮚ໬࢔ࣃࢱ࢖ࢺ㸹nHAp,ࢼࣀ⢏Ꮚ໬๓ࡢ࢔ࣃࢱ࢖ࢺ⢊ᮎ㸹HAp**POs-Ca ྵ᭷෌▼⅊໬⁐ᾮ௨እࡢேᕤၚᾮࡣ࢝ࣝࢩ࣒࢘※࡜ࡋ࡚ CaCl2 ࢆῧຍࡋࡓࠋ***ேᕤၚᾮࡢ࢝ࣝࢩ࣒࢘࡜ࣜࣥ㓟ࡢ⃰ᗘࡣ㻔㻌㻕ෆ࡟♧ࡍῧຍ㔞࡛ㄪᩚࢆ࠾ࡇ࡞ࡗࡓࠋ

70෌▼⅊໬⋡(%)

60
50
40
30
20
100ேᕤၚᾮ䛾䜏

POs-Ca+HAp

POs-CaPOsCa+nHAp(300nm)

POsCa+nHAp(140 nm)

n=5, *SDᅗ 1㸬ྛ෌▼⅊໬⁐ᾮ࡟ࡼࡿ෌▼⅊໬⋡ࢢࣛࣇ

120෌⤖ᬗ໬⋡(%)

1008060
40200ேᕤၚᾮ䛾䜏

POs-Ca+HAp

POs-CaPOsCa+nHAp(300nm)

POsCa+nHAp(140 nm)

n=5, *SDᅗ 2㸬ྛ෌▼⅊໬⁐ᾮ࡟ࡼࡿ෌⤖ᬗ໬⋡ࢢࣛࣇཧ⪃ᩥ⊩㸸[1]Kamasaka, H., Uchida, M., Kusaka, K., Yamamoto, K., Yoshikawa, K., Okada, S., Ichikawa, T. Inhibitoryffectof phosphorylated oligosaccharides prepared from potato starch on the formation of calcium phosphate, Bosci.
Biotech Biochem, 59 : 1412-1416 ,1995[2]H. Kamasaka, D. Inaba, K. Minami, T. Nishimura, T. Kuriki, S. Imai, and M. Yonemitsu : Remineralization ofEnamel by Phosphoryl-Oligosaccharides㻔POs㻕 Supplied by Chewing Gum. Part I. Salivary Assessment invitro. J. Dent. Hlth. 52, 105-111㻌㻔2002㻕..
[3]H. Kamasaka, D. Inaba, K. Minami, T. Nishimura, K. To-o, T. Kuriki, S. Imai,N. Hanada, and M. Yonemitsu :Application of Phosphoryl Oligosaccharides of Calcium 㻔POs-Ca㻕 for Oral Health. J. Appl. Glycosci. 51,129-134㻔2004㻕.
[4]Yagi N, Ohta N, Matsuo T, Tanaka T, Terada Y, Kamasaka H, et al. Evaluation of enamel crystallites insubsurface lesion by microbeam X-ray diffraction. J Synchrotron Rad 2009; 16:398-404[5]Tomoko Tanaka, Yshinobu Terada, Hisorishi Kamasaka, Takashi Kometani , Takashi Kuriki, and Naoto Yagi.
Correlation analysis between remineralization and recrystallization of subsurface lesion by phosphoryl
oligosaccharides of calcium 㻔POs-Ca®㻕 ORCA congress 2008.