論文紹介
Cell Sheet technology（細胞シート工学）Suface modification（細胞培養皿表面技術関連）UpCell®


Adipose-derived stem / stromal cells（脂肪由来幹細胞／間質細胞）

Adipose tissue-derived stromal vascular fraction (SVF) cells

Bladder（膀胱）Blood outgrowth endothelial cells (BOECs)

Bone Marrow-Derived cell / Bone marrow mesenchymal stromal cells/ Bone marrow stromal cells（骨髄間質細胞）



Cardiac myoblast / Cardiomyocyte（心筋）Cardiac stem cells（心臓幹細胞）

Cardiovascular cells（心臓血管細胞）

Chondrocyte（軟骨）Cornea（角膜）Corneal endthelial cells（角膜内皮細胞）

Dental epithelial (DE) cells/ Dental mesenchymal (DM) cellsEmbryonic stem cells（胚性幹細胞）

Endometrial cells（子宮内膜）

Fibroblast (NIH/3T3)（線維芽細胞）

HCT-116(-luc2) <human colon cancer cell line including luciferase
2> (結腸癌）Hepatocyte（肝臓）Human 293 (CD4 expressing) cells（ヒト 293 細胞）

Human Rotator Cuff-Derived cells（腱板由来細胞）Islet（膵島）

Keratinocyte（表皮）Lung（肺）

Macrophages（マクロファージ）Mesenchymal stem cell（間葉系幹細胞）/ Mesenchymal Stromal cell（間葉系
間質細胞）



Microglia（ミクログリア）Nasal mucosa epithelial cells（鼻粘膜）Oral mucosal epithelial cell（口腔粘膜上皮細胞）

Osteoblasts（骨芽細胞）

Periodontal ligament（歯根膜）Peripheral blood mononuclear cells（末梢血単核球細胞）

Renal cell, MDCK cell（腎臓）Retinal pigment cell（網膜色素上皮）Skeletal myoblast（骨格筋芽）Smooth muscle cell（平滑筋細胞）

Synoviocyte（滑膜細胞）

Tendon derived stem / progenitor cell(TSC)（腱由来幹細胞）

Thyroid（甲状腺）Urothelial cell（尿路上皮細胞）

Vein endothelial cells（血管内皮細胞）

Review



RepCell®

HydroCell®

cellZscopeTM

Cell Sheet technology（細胞シート工学）

Kikuchi, A., Okuhara. M., Karikusa. F., Sakurai. Y., and Okano. T. (1998). Twodimensional manipulation of confluently cultured vascular endothelial cells
usingtemperature-responsivepoly(N-isopropylacrylamide)-grafted

surfaces. Journal of Biomaterials Science, Polymer Edition 9(12), 13311348
Okano, T., Yamada, N., Okuhara, M., Sakai, H., and Sakurai, Y. (1995).
Mechanism of cell detachment from temperature-modulated, hydrophilichydrophobic polymer surfaces. Biomaterials 16, 297-303.
Yamada, N., Okano, T., Sakai, H., Karikusa, F., Sawasaki, Y., and Sakurai, Y.
(1990). Thermo-responsive polymeric surfaces; control of attachment anddetachment of

cultured

cells.

Communication 11, 571-576.

Die Makromolekulare Chemie,

Rapid



Surface modification（細胞培養皿表面技術関連）

Nakajima, R., Kobayashi, T., Moriya, N., Mizutani, M., Kan, K., Nozaki, T.,
Saitoh, K., Yamato, M., Okano, T., Takeda, S. (2015). A novel closed cell
culture device for fabrication of corneal epithelial cell sheets. J Tissue Eng
Regen Med; 9, 1259-1267.
Kumashiro, Y., Fukumori, K., Takahashi, H., Nakayama, M., Akiyama, Y.,
Yamato, M., Okano, T. (2013). Modulation of cell adhesion and detachment
on thermo-responsive polymeric surfaces through the observation of
surface dynamics. Biointerfaces 106, 198-207.
Kumashiro, Y., Itoga, K., Kinoshita, Y., Yamato, M., Okano, T. (2013).
Development of positive photoresist for controlling cell culture shape onorganic substrates. chem. Lett., 42, 741-743.
Matsuzaka, N., Nakayama, M., Takahashi, H., Yamato, M., Kikuchi, A., Okano,
T. (2013). Terminal-functionality effect of poly(N-isopropylacrylamide)
brush surfaces on temperature-controlled cell adhesion/detachment.
Biomacromolecules 14, 3164-3171.
Muraoka, M., Shimizu, T., Itoga, K., Takahashi, H., Okano, T. (2013). Controlof the formation of vascular networks in 3D tissue engineered constructs.
Biomaterials 34, 696-703.
Uesugi, K., Akiyama, Y., Hosono, T., Akiyama, Y., Yamato, M., Okano, T.,
Morishima, K. (2013). Measuring adhesion force of a cell sheet by theninety-degree peel test using a multi hook type fixture. Journal of
Biomechanical Science and Engineering Vol. 8, No. 2.
Takahashi, H., Shimizu, T., Nakayama, M., Yamato, M., Okano, T. (2013). The
use of anisotropic cell sheets to control orientation during the selforganization of 3D muscle tissue. Biomaterials 34, 7372-7380.
Elloumi-Hannachi, I., Maeda, M., Yamato, M. &amp; Okano, T. (2010)
Portable microcontact printing device for cell culture. Biomaterials, 31(34),8974-8979.
Elloumi-Hannachi, I., Itoga, K., Kumashiro, Y., Kobayashi, J., Yamato, M.
&amp; Okano, T. (2009) Fabrication of transferable micropatterned-cocultured cell sheets with microcontact printing. Biomaterials, 30(29),5427-5432.
Hannachi, I. E., Yamato, M. &amp; Okano, T. (2009) Cell sheet technology
and cell patterning for biofabrication. Biofabrication, 1(2), 022002.

Nagase, K., Kobayashi, J. &amp; Okano, T. (2009) Temperature-responsive
intelligent

interfacesforbiomolecularseparation

and

cell

sheet

engineering. J R Soc Interface, 6 Suppl 3, S293-309.
Nishi, M., Kobayashi, J., Pechmann, S., Yamato, M., Akiyama, Y., Kikuchi, A.,
Uchida, K., Textor, M., Yajima, H., and Okano, T. (2007). The use of biotinavidin binding to facilitate biomodification of thermoresponsive culture
surfaces. Biomaterials 28, 5471-5476.
Tsuda, Y., Shimizu, T., Yamato, M., Kikuchi, A., Sasagawa, T., Sekiya, S.,
Kobayashi, J., Chen, G., and Okano, T. (2007). Cellular control of tissue
architectures using a three-dimensional tissue fabrication technique.
Biomaterials 28, 4939-4946.
Hatakeyama, H., Kikuchi, A., Yamato, M., and Okano, T. (2006). Biofunctionalized thermoresponsive interfaces facilitating cell adhesion andproliferation. Biomaterials 27, 5069-5078.
Hatakeyama, H., Kikuchi, A., Yamato, M., and Okano, T. (2005). Influence of
insulin immobilization to thermoresponsive culture surfaces on cellproliferation and thermally induced cell detachment. Biomaterials 26,
5167-5176.
Ebara, M., Yamato, M., Aoyagi, T., Kikuchi, A., Sakai, K., and Okano, T. (2004).
Immobilizationofcell-adhesive

peptidestotemperature-responsive

surfaces facilitates both serum-free cell adhesion and noninvasive cell
harvest. Tissue engineering 10, 1125-1135.
Ebara, M., Yamato, M., Aoyagi, T., Kikuchi, A., Sakai, K., and Okano, T. (2004).
Temperature-responsive cell culture surfaces enable &quot;on-off&quot;
affinity

controlbetweencell

integrins

and

RGDS

ligands.

Biomacromolecules 5, 505-510.
Ebara, M., Yamato, M., Hirose, M., Aoyagi, T., Kikuchi, A., Sakai, K., andOkano, T. (2003). Copolymerization of 2-carboxyisopropylacrylamide with
N-isopropylacrylamide accelerates cell detachment from grafted surfaces
by reducing temperature. Biomacromolecules 4, 344-349.
Kwon, O.H., Kikuchi, A., Yamato, M., and Okano, T. (2003). Accelerated cellsheet recovery by co-grafting of PEG with PIPAAm onto porous cell culture
membranes. Biomaterials 24, 1223-1232.

Kwon, O.H., Kikuchi, A., Yamato, M., Sakurai, Y., and Okano, T. (2000). Rapid
cell sheet detachment from poly(N-isopropylacrylamide)-grafted porous
cell culture membranes. Journal of biomedical materials research 50, 8289.
Yamato, M., Konno, C., Kushida, A., Hirose, M., Utsumi, M., Kikuchi, A., andOkano, T. (2000). Release of adsorbed fibronectin from temperatureresponsive culture surfaces requires cellular activity. Biomaterials 21, 981986.
Uchida, K., Sakai, K., Ito, E., Kwon, O.H., Kikuchi, A., Yamato, M., and Okano,
T. (2000). Temperature-dependent modulation of blood platelet movement
andmorphologyonpoly(N-isopropylacrylamide)-grafted

Biomaterials 21, 923-929.

surfaces.

UpCell®


Adipose-derived stem / stromal cells（脂肪由来幹細胞／間質細胞）

Kim, J., Joo, H., Kim, M., Choi, S., Lee, J., Hong, S., Lim, D. (2016).
Transplantation of adipose-derived stem cell sheet attenuates adverse
cardiac remodeling in acute myocardial infarction. TISSUE ENGINEERING
Part A. DOI: 10.1089/ten.tea.2016.0023
Ishida, O., Hagino, I., Nagaya, N., Shimizu, T., Okano, T., Sawa, Y., Mori, H.,
Yagihara, T. (2015). Adipose-derived stem cell sheet transplantation
therapy in a porcine model of chronic heart failure. Translational Research
165, 631-639.
Sasagawa, T., Shimizu, T., Sekiya, S., Yamato, M., Okano, T. (2014).
Comparison of angiogenic potential between prevascular and nonprevascular layered adipose-derived stem cell-sheets in early posttransplanted period. J Biomed Mater Res Part A 102A, 358-364.
Tatsumi, K., Ohashi, K., Matsubara, Y., Kohori, A., Ohno, T., Kakidachi, H.,
Horii, A., Kanegae, K., Utoh, R., Iwata, T., Okano, T. (2013). Tissue factor
triggers procoagulation in transplanted mesenchymal stem cells leading tothromboembolism. Biochemical and Biophysical Research Communications
431, 203-209.
Watanabe, N., Ohashi, K., Tatsumi, K., Utoh, R., Shim, I., Kanegae, K.,
Kashiwakura, Y., Ohmori, T, Sakata, Y., Inoue, M., Hasegawa, M., Okano, T.,(2013). Genetically modified adipose tissue-derived stem/stromal cells,
using simian immunodeficiency virus-based lentiviral vectors, in thetreatment of hemophilia B. HUMAN GENE THERAPY 24, 283-294.
Sasagawa, T., Shimizu, T., Sekiya, S., Yamato, M., Okano, T. (2013).
Comparison of angiogenic potential between prevascular and nonprevascular layered adipose-derived stem cell-sheets in early posttransplanted period. J Biomed Mater Res Part A, 102A: 358-365.
Watanabe, N., Ohashi, K., Tatsumi, K., Utoh, R., Shim, I., Kanegae, K.,
Kashiwakura, Y., Ohmori, T., Sakata, Y., Inoue, M., Hasegawa, M., Okano, T.
(2013). Genetically modified adipose tissue-derived stem/stromal cells,
using simian immunodeficiency virus-based lentiviral vectors, in thetreatment of hemophilia B. HUMAN GENE THERAPY 24, 283-294.

Hamdi, H., Planat-Benard, V., Bel, A., Puymirat, E., Geha, R., Pidial, L.,
Nematalla, H., Bellamy, V., Bouaziz, P., Peyrard, S., Casteilla, L., Bruneval, P.,
Hagege, A. A., Agbulut, O. &amp; Menasche, P. (2011) Epicardial adipose
stem

cell

sheetsresults

ingreaterpost-infarction

intramyocardial injections. Cardiovasc Res, 91(3), 483-491.

survivalthanAdipose tissue-derived stromal vascular fraction (SVF) cells

Kamata, S., Miyagawa, S., Fukushima, S., Imanishi, Y., Saito, A., Maeda, N.,
Shimomura, I., Sawa, Y. (2015). Targeted delivery of adipocytokines into
the heart by induced adipocyte cell-sheet transplantation yields immune
tolerance and functional recovery in autoimmune-associated myocarditis inrats. Circ J 79, 169-179



Bladder（膀胱）Watanabe, E., Yamato, M., Shiroyanagi, Y., Tanabe, K. &amp; Okano, T.
(2011) Bladder augmentation using tissue-engineered autologous oral
mucosal epithelial cell sheets grafted on demucosalized gastric flaps.
Transplantation, 91(7), 700-706.



Blood outgrowth endothelial cells (BOECs)

Tatsumi, K., Sugimoto, M., Lillicrap, D., Shima, M., Ohashi, K., Okano, T.,
Matsui, H. (2013). A novel cell-sheet technology that achieves durable
factor viii delivery in a mouse model of hemophilia A. PLos ONE 8(12):e83280.

Bone Marrow-Derived cell / Bone marrow mesenchymal stromal cells/ Bone marrow stromal cells（骨髄間質細胞）

Imamura, T., Ogawa, T., Minagawa, T., Yokoyama, H., Nakazawa, M.,
Nishizawa, O., Ishizuka, O. (2015). Engineered bone marrow-derived cellsheets restore structure and function of radiation-injured rat urinary
bladders. TISSUE ENGINEERING Part A Volume 21 Numbers 9,
Ito, M., Shichinohe, H., Houkin, K., Kuroda, S. (2014). Application of cellsheet technology to bone marrow stromal cell transplantation for rat brain
infarct. J Tissue Eng Regen Med<
Tano, N., Narita, T., Kaneko, M., Ikebe, C., Coppen, S., Campbell, N., Shiraishi,
M., Shintani, Y., Suzuki, K. (2014). Epicardial placement of mesenchymal
stromal cell-sheets for the treatment of ischemic cardiomyopathy; in vivo
proof-of-concept study. Moleculartherapy vol. 22 no. 10, 1864-1871.



Cardiac myoblast / Cardiomyocyte（心筋）Matsuura, K., Kodama, F., Sugiyama, K., Shimizu, T., Hagiwara, N., Okano, T.
(2015). Elimination of remaining undifferentiated induced pluripotent stem
cells in the process of human cardiac cell sheet fabrication using amethionine-free culture condition. TISSUE ENGINEERING Part C, Volume
21, Number 3.
Komae, H., Sekine, H., Dobashi, I., Matsuura, K., Ono, M., Okano, T., Shimizu,T.

(2015).

Three-dimensional

functionalhuman

myocardial

tissuesfabricated from induced pluripotent stem cells. J Tissue Eng Regen Med.
DOI: 10.1002/term.1995
Hasegawa, A., Haraguchi, Y., Shimizu, T., Okano, T. (2015). Rapid fabrication
system for three-dimensional tissues using cell sheet engineering andcentrifugation. J Biomed Mater Res Part A 103A, 3825-3833.
Sakaguchi, K., Shimizu, T., Horaguchi, S., Sekine, H., Yamato, M., Umezu, M.,
Okano, T. (2013). In vitro engineering of vascularized tissue surrogates.
Sci. Rep. 3, 1316.
Sekine, H., Shimizu, T., Sakaguchi, K., Dobashi, I., Wada, M., Yamato, M.,
Kobayashi, E., Umezu, M., Okano, T. (2013). In vitro fabrication of functional
three-dimensional tissues with perfusable blood vessels. Nat. Commun. 4,1399.
Shioyama, T., Haraguchi, Y., Muragaki, Y., Shimizu, T., Okano, T. (2013). New
isolation system for collecting living cells from tissue. Journal of Bioscienceand Bioengineering VOL. 115 No. 1, 100-103.
Fu, X., Lee, J., Miwa, K., Shimizu, T., Takagishi, Y., Hirabayashi, M., Watabe,K., Usui, A., Kodama, I., Ueda, Y. (2013). Sympathetic innervation induced
in engrafted engineered cardiomyocyte sheets by glial cell line derived
neurotrophic

factorin

vivo.

BioMedResearch

International

doi.org/10.1155/2013/532720
Kawamura, M., Miyagawa, S., Fukushima, S., Saito, A., Miki, K., Ito, E.,
Sougawa, N., Kawamura, T., Daimon, T., Shimizu, T., Okano, T., Toda, K.,
Sawa, Y. (2013). Enhanced survival of transplanted human induced
pluripotent stem cell-derived cardiomyocytes by the combination of cellsheets with the pedicled omental flap technique in a porcine heart.
Circulation 128, S87-S94.

Sekine, H., Shimizu, T., Dobashi, I., Matsuura, K., Hagiwara, N., Takahashi,M., Kobayashi, E., Yamato, M., Okano, T. (2011). Cardiac cell sheettransplantation improves damaged heart function via superior cell survival
in comparison with dissociated cell injection. TISSUE ENGINEERING Part A,
Volume 17, Numbers 23 and 24.
Siltanen, A., Kitabayashi, K., Lakkisto, P., Makela, J., Patila, T., Ono, M.,
Tikkanen, I., Sawa, Y., Kankuri, E. &amp; Harjula, A. (2011) Hhgf
overexpression in myoblast sheets enhances their angiogenic potential in
rat chronic heart failure. PLoS One, 6(4), e19161.
iltanen, A., Kitabayashi, K., Patila, T., Ono, M., Tikkanen, I., Sawa, Y., Kankuri,
E. &amp; Harjula, A. (2011) Bcl-2 improves myoblast sheet therapy in rat
chronic heart failure. Tissue Eng Part A, 17(1-2), 115-125.
Haraguchi, Y., Shimizu, T., Yamato, M. &amp; Okano, T. (2010) Electricalinteraction

betweencardiomyocyte

sheetsseparated

by

non-

cardiomyocyte sheets in heterogeneous tissues. J Tissue Eng Regen Med,
4(4), 291-299.
Hata, H., Bar, A., Dorfman, S., Vukadinovic, Z., Sawa, Y., Haverich, A. &amp;
Hilfiker, A. (2010) Engineering a novel three-dimensional contractile
myocardial patch with cell sheets and decellularised matrix. Eur J
Cardiothorac Surg, 38(4), 450-455.
Kitabayashi, K., Siltanen, A., Patila, T., Mahar, M. A., Tikkanen, I., Koponen,J., Ono, M., Sawa, Y., Kankuri, E. &amp; Harjula, A. (2010) Bcl-2 expression
enhances myoblast sheet transplantation therapy for acute myocardial
infarction. Cell Transplant, 19(5), 573-588.
Shimizu, K., Fujita, H. &amp; Nagamori, E. (2010) Micropatterning of single
myotubes on a thermoresponsive culture surface using elastic stencil
membranes for single-cell analysis. J Biosci Bioeng, 109(2), 174-178.
Sasagawa, T., Shimizu, T., Sekiya, S., Haraguchi, Y., Yamato, M., Sawa, Y.
&amp; Okano, T. (2010) Design of prevascularized three-dimensional celldense tissues using a cell sheet stacking manipulation technology.
Biomaterials, 31(7), 1646-1654.
Shimizu, T., Sekine, H., Yamato, M. &amp; Okano, T. (2009) Cell sheet-based
myocardial tissue engineering: New hope for damaged heart rescue. Curr
Pharm Des, 15(24), 2807-2814.

Matsuura, K., Honda, A., Nagai, T., Fukushima, N., Iwanaga, K., Tokunaga, M.,
Shimizu, T., Okano, T., Kasanuki, H., Hagiwara, N. &amp; Komuro, I. (2009)
Transplantation of cardiac progenitor cells ameliorates cardiac dysfunction
after myocardial infarction in mice. J Clin Invest, 119(8), 2204-2217.
Okura, H., Matsuyama, A., Lee, C. M., Saga, A., Kakuta-Yamamoto, A., Nagao,
A., Sougawa, N., Sekiya, N., Takekita, K., Shudo, Y., Miyagawa, S., Komoda,
H.,

Okano,

T.

&amp;

Sawa,

Y.

(2009)

Cardiomyoblast-likecells

differentiated from human adipose tissue-derived mesenchymal stem cells
improve left ventricular dysfunction and survival in a rat myocardial
infarction model. Tissue Eng Part C Methods, 16(3), 417-425.
Sekiya, N., Matsumiya, G., Miyagawa, S., Saito, A., Shimizu, T., Okano, T.,
Kawaguchi, N., Matsuura, N. &amp; Sawa, Y. (2009) Layered implantationof myoblast sheets attenuates adverse cardiac remodeling of the infarcted
heart. J Thorac Cardiovasc Surg, 138(4), 985-993.
Masuda, S., Shimizu, T., Yamato, M., and Okano, T. (2008). Cell sheetengineering for heart tissue repair. Advanced drug delivery reviews 60,
277-285.
Shimizu, T., Sekine, H., Yang, J., Isoi, Y., Yamato, M., Kikuchi, A., Kobayashi,E., and Okano, T. (2006). Polysurgery of cell sheet grafts overcomes
diffusion limits to produce thick, vascularized myocardial tissues. The
FASEB journal 20, 708-710.
Shimizu, T., Sekine, H., Isoi, Y., Yamato, M., Kikuchi, A., and Okano, T. (2006).
Long-term survival and growth of pulsatile myocardial tissue grafts
engineered by the layering of cardiomyocyte sheets. Tissue engineering 12,499-507.
Sekine, H., Shimizu, T., Yang, J., Kobayashi, E., and Okano, T. (2006).
Pulsatile

myocardial

tubes

fabricated

with

cell

sheet

engineering.

Circulation 114, I87-93.
Sekiya, S., Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2006).
Bioengineered cardiac cell sheet grafts have intrinsic angiogenic potential.
Biochemical and biophysical research communications 341, 573-582.
Sekine, H., Shimizu, T., Kosaka, S., Kobayashi, E., and Okano, T. (2006).
Cardiomyocyte bridging between hearts and bioengineered myocardial
tissues with mesenchymal transition of mesothelial cells. J Heart Lung
Transplant 25, 324-332.

Haraguchi, Y., Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2006).
Electrical coupling of cardiomyocyte sheets occurs rapidly via functional
gap junction formation. Biomaterials 27, 4765-4774.
Furuta, A., Miyoshi, S., Itabashi, Y., Shimizu, T., Kira, S., Hayakawa, K.,
Nishiyama, N., Tanimoto, K., Hagiwara, Y., Satoh, T., Fukada, K., Okano, T.
and Ogawa, S. (2006). Pulsatile cardiac tissue grafts using a novel threedimensional cell sheet manipulation technique functionally integrates withthe host heart, in vivo. Circulation research 98, 705-712.
Itabashi, Y., Miyoshi, S., Yuasa, S., Fujita, J., Shimizu, T., Okano, T., Fukuda,K., and Ogawa, S. (2005). Analysis of the electrophysiological properties
and arrhythmias in directly contacted skeletal and cardiac muscle cellsheets. Cardiovascular research 67, 561-570.
Miyagawa, S., Sawa, Y., Sakakida, S., Taketani, S., Kondoh, H., Memon, I.A.,
Imanishi, Y., Shimizu, T., Okano, T., and Matsuda, H. (2005). Tissue
cardiomyoplasty using bioengineered contractile cardiomyocyte sheets torepair damaged myocardium: their integration with recipient myocardium.
Transplantation 80, 1586-1595.
Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2003). Cell sheetengineering for myocardial tissue reconstruction. Biomaterials 24, 23092316.
Shimizu, T., Yamato, M., Isoi, Y., Akutsu, T., Setomaru, T., Abe, K., Kikuchi, A.,
Umezu, M., and Okano, T. (2002). Fabrication of pulsatile cardiac tissue
grafts using a novel 3-dimensional cell sheet manipulation technique andtemperature-responsive cell culture surfaces. Circulation research 90,
e40-e48.
Shimizu, T., Yamato, M., Akutsu, T., Shibata, T., Isoi, Y., Kikuchi, A., Umezu,
M., and Okano, T. (2002). Electrically communicating three-dimensional
cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets.
Journal of biomedical materials research 60, 110-117.
Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2001). Two-dimensional
manipulation of cardiac myocyte sheets utilizing temperature-responsive
culture dishes augments the pulsatile amplitude. Tissue Engineering 7,
141-151.



Cardiac stem cells（心臓幹細胞）

Kamata, S., Miyagawa, S., Fukushima, S., Nakatani, S., Kawamoto, A., Saito,
A., Harada, A., Shimizu, T., Daimon, T., Okano, T., Asahara, T., Sawa, Y.
(2014). Improvement of cardiac stem cell sheet therapy for chronic
ischemic injury by adding endothelial progenitor cell transplantation:
analysis of layer-specific regional cardiac function. Cell Transplantation
Vol 23, 1305-1319.



Cardiovascular cells（心臓血管細胞）

Masumoto, H., Ikuno, T., Takeda, M., Fukushima, H., Marui, A., Katayama, S.,
Shimizu, T., Ikeda, T., Okano, T., Sakata, R., Yamashita, J. (2014). Human iPS
cell-engineered cardiac tissue sheets with cardiomyocytes and vascular
cells

for

cardiac10.1038/srep06716

regeneration.

SCIENTIFICREPORTS.

DOI:Chondrocyte（軟骨）Shimizu, R., Kamei, N., Adachi, N., Hamanishi, M., Kamei, G., Mahmoud, E.,
Nakano, T., Iwata, T., Yamato, M., Okano, T., Ochi, M. (2015). Repair
mechanismofosteochondral

defectpromotedbybioengineeredchondrocyte sheet. TISSUE ENGINEERING Part A Volume 21, Numbers 5 &6.
Shudo, Y., Cohen, J., MacArthur, J., Goldstone, A., Otsuru, S., Trubelja, A.,
Patel, J., Edwards, B., Hung, G., Fairman, A., Brusalis, C., Hiesinger, W., Atluri,P., Hiraoka, A., Miyagawa, S., Sawa, Y., Woo, Y. (2015). A tissue-engineeredchondrocyte cell sheet induces extracellular matrix modification toenhance ventricular biomechanics and attenuate myocardial stiffness inischemic cardiomyopathy. TISSUE ENGINEERING Part A, Volume 21
Numbers 19 & 20.
Takaku, Y., Murai, K., Ukai, T., Ito, S., Kokubo, M., Satoh, M., Kobayashi, E.,
Yamato, M., Okano, T., Takeuchi, M., Mochida, J., Sato, M. (2014). In vivo celltrackingbybioluminescenceimaging

after

transplantationofbioengineered cell sheets to the knee joint. Biomaterials 35, 2199-2206.
Sato M. (2010) Cell sheet technologies for cartilage repair, Regenerative
medicine and biomaterials for the repair of connective tissues. (Edited byArcher C and Ralphs J) WOODHEAD Publishing Limited, CRC Press LLC, 251265.
Mitani, G., Sato, M., Lee, J. I., Kaneshiro, N., Ishihara, M., Ota, N., Kokubo, M.,
Sakai, H., Kikuchi, T. & Mochida, J. (2009) The properties of bioengineeredchondrocyte sheets for cartilage regeneration. BMC Biotechnol 6, 9-17.
Sato, M., lshihara, M., Furukawa, K., Kaneshiro, N., Nagai, T., Mitani, G.,
Kutsuna, T., 0hta, N., Kokubo, M., Kikuchi, T., Sakai, H., Ushida, T., Kikuchi,
M. and Mochida, J. (2008) Recent technological advancements related to
articular cartilage regeneration. Med BioI Eng Comput 46, 735-743
Kaneshiro. N., Sato. M., Ishihara. M., Mitani. G., Sakai. H., Kikuchi. T. andMochida. J. (2007). Cultured articular chondrocytes sheets for partial
thickness

cartilage

defects

utilizing

temperature-responsive

culturedishes. European Cells and Materials 13, 87-92.
Kaneshiro, N., Sato, M., Ishihara, M., Mitani, G., Sakai, H., and Mochida, J.
(2006). Bioengineered chondrocyte sheets may be potentially useful forthetreatmentofpartialthickness

defectsofarticular

cartilage.

Biochemical and biophysical research communications 349, 723-731.

Cornea（角膜）Kobayashi, T., Yamato, M., Okano, T., Watanabe, Y. (2013). Quantitativeevaluation system for tissue-engineered corneal epithelial cell sheets
using image-based technology. Journal of Regenerative Medicine & Tissue
Engineering, doi: 10.7243/2050-1218-2-2
Takagi, R., Yamato, M., Murakami, D., Kondo, M., Yang, J., Ohki, T., Nishida,K., Kohno, C. & Okano, T. (2011) Preparation of keratinocyte culture
medium for the clinical applications of regenerative medicine. J Tissue Eng
Regen Med, 5(4), e63-73.
Hayashi, R., Yamato, M., Takayanagi, H., Oie, Y., Kubota, A., Hori, Y., Okano,T. & Nishida, K. (2010) Validation system of tissue-engineered epithelial
cell sheets for corneal regenerative medicine. Tissue Eng Part C Methods,
16(4), 553-560.
Watanabe, K., Yamato, M., Hayashida, Y., Yang, J., Kikuchi, A., Okano, T.,
Tano, Y., and Nishida, K. (2007). Development of transplantable geneticallymodified corneal epithelial cell sheets for gene therapy. Biomaterials 28,745-749.
Kanayama, S., Nishida, K., Yamato, M., Hayashi, R., Sugiyama, H., Soma, T.,
Maeda, N., Okano, T., and Tano, Y. (2007). Analysis of angiogenesis inducedby cultured corneal and oral mucosal epithelial cell sheets in vitro.
Experimental eye research 85, 772-781.
Sumide, T., Nishida, K., Yamato, M., Ide, T., Hayashida, Y., Watanabe, K.,
Yang, J., Kohno, C., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T. and Tano,
Y. (2006). Functional human corneal endothelial cell sheets harvested fromtemperature-responsive culture surfaces. The FASEB Journal 20, 392-394.
Ide, T., Nishida, K., Yamato, M., Sumide, T., Utsumi, M., Nozaki, T., Kikuchi,A., Okano, T., and Tano, Y. (2006). Structural characterization of
bioengineered human corneal endothelial cell sheets fabricated on
temperature-responsive culture dishes. Biomaterials 27, 607-614.
Hayashida, Y., Nishida, K., Yamato, M., Yang, J., Sugiyama, H., Watanabe, K.,
Hori, Y., Maeda, N.,

Kikuchi, A., Okano, T. andTano, Y.

(2006).

Transplantation of tissue-engineered epithelial cell sheets after excimer
laser photoablation reduces postoperative corneal haze. Investigative
ophthalmology & visual science 47, 552-557.

Umemoto, T., Yamato, M., Nishida, K., Kohno, C., Yang, J., Tano, Y., andOkano, T. (2005). Rat limbal epithelial side population cells exhibit a
distinct expression of stem cell markers that are lacking in side population
cells from the central cornea. FEBS letters 579, 6569-6574.
Watanabe, K., Nishida, K., Yamato, M., Umemoto, T., Sumide, T., Yamamoto,K., Maeda, N., Watanabe, H., Okano, T., and Tano, Y. (2004). Human limbal
epithelium contains side population cells expressing the ATP-binding
cassette transporter ABCG2. FEBS letters 565, 6-10.
Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Maeda, N., Watanabe,
H., Yamamoto, K., Nagai, S., Kikuchi, A., Tano, Y. and Okano, T. (2004).
Functional bioengineered corneal epithelial sheet grafts from corneal stem
cells expanded ex vivo on a temperature-responsive cell culture surface.
Transplantation 77, 379-385.

Corneal endothelial cells（角膜内皮細胞）

Lai, J. Y. &amp; Li, Y. T. (2010) Functional assessment of cross-linked
porousgelatinhydrogelsforbioengineered

Biomacromolecules, 11(5), 1387-1397.

cell

sheet

carriers.

Dental epithelial (DE) cells/ Dental mesenchymal (DM) cells

Monteiro, N., Smith, E., Angstadt, S., Zhang, W., Khademhosseini, A., Yelick,
P. (2016). Dental cell sheet biomimetic tooth bud model. Biomaterials 106,
167-179.



Embryonic stem cells（胚性幹細胞）

Matsuura, K., Masuda, S., Haraguchi, Y., Yasuda, N., Shimizu, T., Hagiwara,N., Zandstra, P. W. &amp; Okano, T. (2011) Creation of mouse embryonicstem cell-derived cardiac cell sheets. Biomaterials, 32(30), 7355-7362.
Bel, A., Planat-Bernard, V., Saito, A., Bonnevie, L., Bellamy, V., Sabbah, L.,
Bellabas, L., Brinon, B., Vanneaux, V., Pradeau, P., Peyrard, S., Larghero, J.,
Pouly, J., Binder, P., Garcia, S., Shimizu, T., Sawa, Y., Okano, T., Bruneval, P.,
Desnos, M., Hagege, A. A., Casteilla, L., Puceat, M. &amp; Menasche, P.
(2010) Composite cell sheets: A further step toward safe and effective
myocardial regeneration by cardiac progenitors derived from embryonicstem cells. Circulation, 122(11 Suppl), S118-123.

Endometrial cells（子宮内膜）

Takagi, S., Shimizu, T., Kuramoto, G., Ishitani, K., Matsui, H., Yamato, M.,
Okano, T. (2014). Reconstruction of functional endometrium-like tissue invitro and in vivo using cell sheet engineering. Biochemical and BiophysicalResearch Communications 446, 335-340.



Fibroblast (NIH/3T3)（線維芽細胞）

Matsushima, H., Kuroki, T., Adachi, T., Kitasato, A., Ono, S., Tanaka, T.,
Hirabaru, M., Kuroshima, N., Hirayama, T., Sakai, Y., Soyama, A., Hidaka, M.,
Takatsuki, M., Kin, T., Shapiro, J., Eguchi, S. (2016). Human fibroblast sheet
promotes human pancreatic islet survival and function in vitro. Cell
Transplantation Vol. 25, 1525-1537.
Baimakhanov, Z., Yamanouchi, K., Sakai, Y., Koike, M., Soyama, A., Hidaka,M., Takatsuki, M., Fujita, F., Kanetaka, K., Kuroki, T., Eguchi, S. (2016).
Efficacy of multilayered hepatocyte sheet transplantation for radiationinduced liver damage and partial hepatectomy in a rat model. Cell
Transplantation Vol. 25, 549-558.
Sakai, Y., Koike, M., Soyama, A., Hidaka, M., Kuroki, T., Eguchi, S. (2015).
Rapid production of engineered human primary hepatocyte/fibroblast
sheets. Data in Brief 5, 498-501.
Sasagawa, T., Shimizu, T., Yamato, M., Okano, T. (2014). Expression profilesof angiogenesis-related proteins in prevascular three-dimensional tissue
using cell-sheet engineering. Biomaterials 35, 206-213.
Sugibayashi, K., Kumashiro, Y., Shimizu, T., Kobayashi, J., Okano, T. (2012).
A molded hyaluronic acid gel as a micro-template for blood capillaries.
Journal of Biomaterials Sciences, Polymer Edition, 24:2, 135-147.
Lee, E. L. &amp; Von Recum, H. A. (2010) Cell culture platform withmechanical conditioning and nondamaging cellular detachment. J Biomed
Mater Res A, 93(2), 411-418.



HCT-116(-luc2) <human colon cancer cell line including luciferase
2> (結腸癌）

Suzuki, R., Aruga, A., Kobayashi, H., Yamato, M., Yamamoto, M. (2014).
Development of a novel in vivo cancer model using cell sheet engineering.
ANTICANCER RESEARCH 34, 4747-4754.



Hepatocyte（肝臓）Baimakhanov, Z., Yamanouchi, K., Sakai, Y., Koike, M., Soyama, A., Hidaka,M., Takatsuki, M., Fujita, F., Kanetaka, K., Kuroki, T., Eguchi, S. (2016).
Efficacy of multilayered hepatocyte sheet transplantation for radiationinduced liver damage and partial hepatectomy in a rat model. celltransplantation Vol. 25, 549-558.
Sakai, Y., Koike, M., Soyama, A., Hidaka, M., Kuroki, T., Eguchi, S. (2015).
Rapid production of engineered human primary hepatocyte/fibroblast
sheets. Data in Brief 5, 498-501.
Sakai, Y., Koike, M., Hasegawa, H., Yamanouchi, K., Soyama, A., Takatsuki,M., Kuroki, T., Ohashi, K., Okano, T., Eguchi, S. (2013). Rapid fabricating
technique for multi-layered human hepatic cell sheets by forcefulcontraction of the fibroblast monolayer. PLoS ONE 8(7), e70970.
Ohno, M., Motojima, K., Okano, T. &amp; Taniguchi, A. (2009) Maturation of
the extracellular matrix and cell adhesion molecules in layered co-culturesof hepg2 and endothelial cells. J Biochem, 145(5), 591-597.
Ohno, M., Motojima, K., Okano, T. &amp; Taniguchi, A. (2009) Induction of
drug-metabolizing enzymes by phenobarbital in layered co-culture of ahuman liver cell line and endothelial cells. Biol Pharm Bull, 32(5), 813-817.
Takayama, G., Taniguchi, A., and Okano, T. (2007). Identification ofdifferentially expressed genes in hepatocyte/endothelial cell co-culture
system. Tissue engineering 13, 159-166.
Ohashi, K., Yokoyama, T., Yamato, M., Kuge, H., Kanehiro, H., Tsutsumi, M.,
Amanuma, T., Iwata, H., Yang, J., Okano, T. and Nakajima, Y. (2007).
Engineering functional two- and three-dimensional liver systems in vivousing hepatic tissue sheets. Nat Med 13, 880-885.
Kurosawa, Y., Taniguchi, A., and Okano, T. (2005). Novel method to examine
hepatocyte-specific gene expression in a functional coculture system.
Tissue engineering 11, 1650-1657.
Harimoto, M., Yamato, M., Hirose, M., Takahashi, C., Isoi, Y., Kikuchi, A., andOkano, T. (2002). Novel approach for achieving double-layered cell sheets
co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes
utilizing temperature-responsive culture dishes. Journal of biomedical
materials research 62, 464-470.

Hirose, M., Yamato, M., Kwon, O.H., Harimoto, M., Kushida, A., Shimizu, T.,
Kikuchi, A., and Okano, T. (2000). Temperature-responsive surface fornovel co-culture systems of hepatocytes with endothelial cells: 2-D
patterned and double layered co-cultures. Yonsei medical journal 41, 803813.



Human 293 (CD4 expressing) cells

Long, Y., Meng, F., Kondo, N., Iwamoto, A. &amp; Matsuda, Z. (2011)
Conserved arginine residue in the membrane-spanning domain of hiv-1
gp41 is required for efficient membrane fusion. Protein Cell, 2(5), 369376.
Kondo, N., Miyauchi, K., Meng, F., Iwamoto, A. &amp; Matsuda, Z. (2010)
Conformational changes of the hiv-1 envelope protein during membranefusion are inhibited by the replacement of its membrane-spanning domain.
J Biol Chem, 285(19), 14681-14688.



Human Rotator Cuff-Derived cells（腱板由来細胞）

Harada, Y., Mifune, Y., Inui, A., Sakata, R., Muto, T., Takase, F., Ueda, Y.,
Kataoka, T., Kokubu, T., Kuroda, R., Kurosaka, M. (2016). Rotator cuff repair
using cell sheets derived from human rotator cuff in a rat model. J Orthop
Res. DOI 10.1002/jor.23289



Islet（膵島）Yamashita, S., Ohashi, K., Utoh, R., Okano, T., Yamamoto, M. (2015). Humanlaminin isotype coating for creating islet cell sheets. Cell Medicine Vol. 8,39-46.
Shimizu, H., Ohashi, K., Saito, T., Utoh, R., Ise, K., Yamato, M., Okano, T.,
Gotoh, M. (2013). Topographical arrangement of α-and &beta;-cells within
neo-islet tissue engineered by islet cell sheet transplantation in mice.
Transplantation Proceedings 45, 1881-1884.
Shimizu. H., Ohashi. K., Utoh. R., Ise. K., Gotoh. M., Yamato. M. and Okano. T.
(2009). Bioengineering of a functional sheet of islet cells for the treatment
of diabetes mellitus. Biomaterials 30, 5943-5949



Keratinocyte（表皮）Osada, A., Sekine, H., Soejima, K., Sakurai, H., Shimizu, T. (2016). Harvestingepithelial

keratinocyte

sheetsfromtemperature-responsive

dishespreserves basement membrane proteins and improves cell survival in a skindefect model. J Tissue Eng Regen Med,Fujisawa, D., Sekine, H., Okano, T., Sakurai, H., Shimizu, T. (2015). Ex vivo
prefabricated rat skin flap using cell sheets and an arteriovenous vascularbundle. Plast Reconstr Surg Glob Open, 3:e424.
Yamato, M., Utsumi, M., Kushida, A., Konno, C., Kikuchi, A., and Okano, T.
(2001). Thermo-responsive culture dishes allow the intact harvest ofmultilayered keratinocyte sheets without dispase by reducing temperature.
Tissue engineering 7, 473-480.



Lung（肺）Kanzaki, M., Yamato, M., Yang, J., Sekine, H., Kohno, C., Takagi, R.,
Hatakeyama, H., Isaka, T., Okano, T., and Onuki, T. (2007). Dynamic sealingof lung air leaks by the transplantation of tissue engineered cell sheets.
Biomaterials 28, 4294-4302.
Kanzaki, M., Yamato, M., Hatakeyama, H., Kohno, C., Yang, J., Umemoto, T.,
Kikuchi, A., Okano, T., and Onuki, T. (2006). Tissue engineered epithelial
cell sheets for the creation of a bioartificial trachea. Tissue engineering
12, 1275-1283.
Nandkumar, M.A., Yamato, M., Kushida, A., Konno, C., Hirose, M., Kikuchi, A.,
and Okano, T. (2002). Two-dimensional cell sheet manipulation ofheterotypically co-cultured lung cells utilizing temperature-responsive
culture dishes results in long-term maintenance of differentiated epithelial
cell functions. Biomaterials 23, 1121-1130.



Macrophages（マクロファージ）

Kato, C. &amp; Kojima, N. (2010) Signr1 ligation on murine peritoneal
macrophages induces il-12 production through nfkappab activation.
Glycoconj J, 27(5), 525-531.

Mesenchymal stem cell（間葉系幹細胞）/Mesenchymal Stromal cell（間葉系
間質細胞）

Pangesty, A., Arahira, T., Todo, M. (2016). Characterization of tensile
mechanical behavior of MSCs/PLCL hybrid layered sheet. J. Funct.
Biomater. 7, 14,
Chang, D., Shimizu, T., Haraguchi, Y., Gao, S., Sakaguchi, K., Umezu, M.,
Yamato, M., Liu, Z., Okano, T. (2015). Time course of cell sheet adhesion to
porcineheart

tissueafter

transplantation.

PLOS

ONE

DOI:10.1371/journal.pone.0137494
Kawamura, M., Miyagawa, S., Fukushima, S., Saito, A., Toda, K., Daimon, T.,
Shimizu, T., Okano, T., Sawa, Y. (2015). Xenotransplantation of bonemarrow-derived human mesenchymal stem cell sheets attenuates left
ventricular remodeling in a porcine ischemic cardiomyopathy model.
TISSUE ENGINEERING Part A, Volume 21, Numbers 15 and 16.
Tano, N., Narita, T., Kaneko, M., Ikebe, C., Coppen, S., Campbell, N., Shiraishi,
M., Shintani, Y., Suzuki, K. (2014). Epicardial placement of mesenchymal
stromal cell-sheets for the treatment of ischemic cardiomyopathy; in vivo
proof-of-concept study. Moleculartherapy vol. 22 no. 10, 1864-1871.
Tatsumi, K., Ohashi, K., Matsubara, Y., Kohori, A., Ohno, T., Kakidachi, H.,
Horii, A., Kanegae, K., Utoh, R., Iwata, T., Okano, T. (2013). Tissue factor
triggers procoagulation in transplanted mesenchymal stem cells leading tothromboembolism. Biochemical and Biophysical Research Communications
431, 203-209.
Miyahara, Y., Nagaya, N., Kataoka, M., Yanagawa, B., Tanaka, K., Hao, H.,
Ishino, K., Ishida, H., Shimizu, T., Kangawa, K., Sano, S., Okano, T., Kitamura,S. and Mori, H. (2006). Monolayered mesenchymal stem cells repair scarred
myocardium after myocardial infarction. Nature medicine 12, 459-465.



Microglia（ミクログリア）

Nakajima, K., Honda, S., Nakamura, Y., Lopez-Redondo, F., Kohsaka, S.,
Yamato, M., Kikuchi, A., and Okano, T. (2001). Intact microglia are cultured
and non-invasively harvested without pathological activation using a novel
cultured cell recovery method. Biomaterials 22, 1213-1223.



Nasal mucosa epithelial cells（鼻粘膜）Hama, T., Yamamoto, K., Yaguchi, Y., Murakami, D., Sasaki, H., Yamato, M.,
Okano, T., Kojima, H. (2015). Autologous human nasal epithelial cell sheet
using temperature-responsive culture insert for transplantation after
middle ear surgery. J Tissue Eng Regen Med.
Yamamoto, K., Hama, T., Yamato, M., Uchimizu, H., Sugiyama, H., Takagi, R.,
Yaguchi, Y., Okano, T., Kojima, H. (2015). The effect of transplantation ofnasal mucosal epithelial cell sheets after middle ear surgery in a rabbit
model. Biomaterials 42, 87-93.



Oral mucosal epithelial cell（口腔粘膜上皮細胞）

Bardag-Gorce, F., Hoft, R., Wood, A., Oliva, J., Niihara, H., Makalinao, A.,
Thropay, J., Pan, D., Meepe, I., Tiger, K., Garcia, J., Laporte, A., French, S.,
Niihara, Y. (2016). The role of E-cadherin in maintaining the barrier
function of corneal epithelium after treatment with cultured autologous
oral mocosa epithelial cell sheet grafts for limbal stem deficiency. Journalof Ophthalmology. DOI.org/10.1155/2016/4805986
Kuramoto, G., Takagi, S., Ishitani, K., Shimizu, T., Okano, T., Matsui, H.
(2015). Preventive effect of oral mucosal epithelial cell sheets on
intrauterine adhesions. Human Reproduction Vol. 30 No. 2, 406-416.
Kocaba, V., Thepot, A., Yamato, M., Daisuke, M., Kellal, M., Mojallal, A.,
Damour, O., &amp; Burillon, C. (2014) Long-Term Results of Cultured
Autologous Oral Mucosa Epithelial Cell-Sheet (CAOMECS) Graft for theTreatment of Blindness Due to Bilateral Limbal Stem Cell Deficiency. J Stem
Cell Res Ther 4(3):181
Kondo, M., Yamato, M., Takagi, R., Namiki, H., Okano, T. (2013). The
regulation of epithelial cell proliferation and growth by IL-1 receptor
antagonist. Biomaterials 34, 121-129.
Tanaka, N., Kondo, M., Uchida, R., Kaneko, M., Sugiyama, H., Yamato, M.,
Okano, T. (2013). Splitting culture medium by air-jet and rewetting for theassessment of the wettability of cultured epithelial cell surfaces.
Biomaterials 34, 9082-9088.
Burillon, C., Huot, L., Justin, V., Nataf, S., Chapuis, F., Decullier, E., &amp;
Damour, O. (2012) Cultured autologous oral mucosal epithelial cell sheet(CAOMECS) transplantation for the treatment of corneal limbal epithelial
stem cell deficiency. Investigative Ophthalmology &amp; Visual Science,
53(3), 1325-31.
Ohki, T., Yamamoto, M., Ota, M., Okano, T. &amp; Yamamoto, M. (2011)
Application

of

cell

sheet

technology

foresophageal

endoscopic

submucosal dissection. Tech Gastrointest Endosc, 13(1), 105-109.
Takagi, R., Murakami, D., Kondo, M., Ohki, T., Sasaki, R., Mizutani, M., Yamato,M., Nishida, K., Namiki, H., Yamamoto, M. &amp; Okano, T. (2010) Fabricationof human oral mucosal epithelial cell sheets for treatment of esophageal
ulceration by endoscopic submucosal dissection. Gastrointest Endosc,72(6), 1253-1259.

Thepot, A., Morel, A. P., Justin, V., Desanlis, A., Thivillier, L., Hoffman, E., Till,
M., Accardi, R., Tommasino, M., Breton, P., Hainaut, P. &amp; Damour, O.
(2010) Evaluation of tumorigenic risk of tissue-engineered oral mucosal
epithelial cells by using combinational examinations. Cell Transplant, 19(8),999-1006.
Ohki, T., Yamato, M., Murakami, D., Takagi, R., Yang, J., Namiki, H., Okano, T.,
and Takasaki, K. (2006). Treatment of oesophageal ulcerations using
endoscopic transplantation of tissue-engineered autologous oral mucosal
epithelial cell sheets in a canine model. Gut 55, 1704-1710.
Murakami, D., Yamato, M., Nishida, K., Ohki, T., Takagi, R., Yang, J., Namiki,H., and Okano, T. (2006). The effect of micropores in the surface oftemperature-responsive

cultureinsertsonthefabricationoftransplantable canine oral mucosal epithelial cell sheets. Biomaterials 27,
5518-5523.
Murakami, D., Yamato, M., Nishida, K., Ohki, T., Takagi, R., Yang, J., Namiki,H., and Okano, T. (2006). Fabrication of transplantable human oral mucosal
epithelial cell sheets using temperature-responsive culture inserts without
feeder layer cells. Journal of artificial organs 9, 185-191.
Hayashida, Y., Nishida, K., Yamato, M., Watanabe, K., Maeda, N., Watanabe,
H.,

Kikuchi,A.,

Okano,

T.,

and

Tano,

Y.

(2005).

Ocularsurfacereconstruction using autologous rabbit oral mucosal epithelial sheets
fabricated

ex

vivo

on

a

temperature-responsive

culturesurface.

Investigative ophthalmology &amp; visual science 46, 1632-1639.
Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Yamamoto, K., Adachi,E., Nagai, S., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T. and Tano, Y.
(2004).

Cornealreconstruction

withtissue-engineeredcellsheetscomposed of autologous oral mucosal epithelium. The New England journalof medicine 351, 1187-1196.



Osteoblasts（骨芽細胞）

Uchiyama, H., Yamato, M., Sasaki, R., Sekine, H., Yang, J., Ogiuchi, H., Ando,
T. &amp; Okano, T. (2011) In vivo 3d analysis with micro-computed
tomography of rat calvaria bone regeneration using periosteal cell sheets
fabricated on temperature-responsive culture dishes. J Tissue Eng Regen
Med, 5(6), 483-490.



Periodontal ligament（歯根膜）Panduwawala, C., Zhan, X., Dissanayaka, W., Samaranayake, L, Jin, L, Zhang,
C. (2016). In vivo periodontal tissue regeneration by periodontal ligament
stem cells and endothelial cells in three-dimensional cell sheet constructs.
J Periodont Res doi:10.1111/jre.12405
Wei, F., Qu, C., Song, T., Ding, G., Fan, Z., Liu, D., Liu, Y., Zhang, C., Shi, S.,
Wang, S. (2012). Vitamin C treatment promotes mesenchymal stem cellsheet formation and tissue regeneration by elevating telomerase activity. J
Cell Physiol. 227(9), 3216-3224.
Izumi, Y., Aoki, A., Yamada, Y., Kobayashi, H., Iwata, T., Akizuki, T., Suda, T.,
Nakamura, S., Wara-Aswapati, N., Ueda, M. &amp; Ishikawa, I. (2011)
Current and future periodontal tissue engineering. Periodontol 2000,
56(1), 166-187.
Tsumanuma, Y., Iwata, T., Washio, K., Yoshida, T., Yamada, A., Takagi, R.,
Ohno, T., Lin, K., Yamato, M., Ishikawa, I., Okano, T. &amp; Izumi, Y. (2011)
Comparison of different tissue-derived stem cell sheets for periodontal
regeneration in a canine 1-wall defect model. Biomaterials, 32(25), 58195825.
Huang, S. &amp; Zhang, D. (2010) Periodontal ligament cell sheetengineering: A new possible strategy to promote periodontal regenerationof dental implants. Dental Hypotheses, 1(1), 23-30.
Washio, K., Iwata, T., Mizutani, M., Ando, T., Yamato, M., Okano, T. &amp;
Ishikawa, I. (2010) Assessment of cell sheets derived from humanperiodontal ligament cells: A pre-clinical study. Cell Tissue Res, 341(3),397-404.
Iwata, T., Yamato, M., Zhang, Z., Mukobata, S., Washio, K., Ando, T., Feijen,
J., Okano, T. &amp; Ishikawa, I. (2010) Validation of human periodontal
ligament-derived cells as a reliable source for cytotherapeutic use. J Clin
Periodontol, 37(12), 1088-1099.
Nakagawa, E., Itoh, T., Yoshie, H. &amp; Satokata, I. (2009) Odontogenic
potential of post-natal oral mucosal epithelium. J Dent Res, 88(3), 219223.
Ishikawa, I., Iwata, T., Washio, K., Okano, T., Nagasawa, T., Iwasaki, K. &amp;
Ando, T. (2009) Cell sheet engineering and other novel cell-based
approaches to periodontal regeneration. Periodontol 2000, 51, 220-238

Iwata, T., Yamato, M., Tsuchioka, H., Takagi, R., Mukobata, S., Washio, K.,
Okano, T. &amp; Ishikawa, I. (2009) Periodontal regeneration with multilayered periodontal ligament-derived cell sheets in a canine model.
Biomaterials, 30(14), 2716-2723.
Gomez Flores, M., Hasegawa, M., Yamato, M., Takagi, R., Okano, T., andIshikawa, I. (2008). Cementum-periodontal ligament complex regeneration
using the cell sheet technique. Journal of periodontal research
Hasegawa, M., Yamato, M., Kikuchi, A., Okano, T., and Ishikawa, I. (2005).
Human periodontal ligament cell sheets can regenerate periodontal
ligament tissue in an athymic rat model. Tissue engineering 11, 469-478.
Akizuki, T., Oda, S., Komaki, M., Tsuchioka, H., Kawakatsu, N., Kikuchi, A.,
Yamato, M., Okano, T., and Ishikawa, I. (2005). Application of periodontal
ligament cell sheet for periodontal regeneration: a pilot study in beagle
dogs. Journal of periodontal research 40, 245-251.

Peripheral blood mononuclear cells（末梢血単核細胞）

Burt, T. D., Seu, L., Mold, J. E., Kappas, A. &amp; Mccune, J. M. (2010) Naive
human t cells are activated and proliferate in response to the heme
oxygenase-1 inhibitor tin mesoporphyrin. J Immunol, 185(9), 5279-5288.

Renal cell, MDCK cell（腎臓）Sekiya, S., Shimizu, T., Yamato, M., Okano, T. (2013). Hormone supplying
renal cell sheet in vivo produced by tissue engineering technology.
BioResearch Open Access Volume 2 Number1.
Kushida, A., Yamato, M., Isoi, Y., Kikuchi, A., and Okano, T. (2005). A
noninvasive transfer system for polarized renal tubule epithelial cell sheets
using temperature-responsive culture dishes. European cells &amp;
materials 10, 23-30; discussion 23-30.
Kushida, A., Yamato, M., Kikuchi, A., and Okano, T. (2001). Two-dimensional
manipulation of differentiated Madin-Darby canine kidney (MDCK) cellsheets: the noninvasive harvest from temperature-responsive culture
dishes and transfer to other surfaces. Journal of biomedical materials
research 54, 37-46.
Kushida, A., Yamato, M., Konno, C., Kikuchi, A., Sakurai, Y., and Okano, T.
(2000).

Temperature-responsive

culturedishesallow

nonenzymatic

harvest of differentiated Madin-Darby canine kidney (MDCK) cell sheets.
Journal of biomedical materials research 51, 216-223.



Yaji,

Retinal pigment epithelial cell（網膜色素上皮細胞）

N.,Yamato,

M.,

Yang,

J.,

Okano,

T.

&amp;

Hori,

S.

(2009)

Transplantation of tissue-engineered retinal pigment epithelial cell sheets
in a rabbit model. Biomaterials, 30(5), 797-803.
Kubota, A., Nishida, K., Yamato, M., Yang, J., Kikuchi, A., Okano, T., and Tano,
Y. (2006). Transplantable retinal pigment epithelial cell sheets for tissue
engineering. Biomaterials 27, 3639-3644.
Abe, T., Hojo, M., Saigo, Y., Yamato, M., Okano, T., Wakusawa, R., and Tamai,
M. (2006). Retinal pigment epithelial cells from thermally responsive
polymer-grafted surface reduce apoptosis. Advances in experimental
medicine and biology 572, 363-366.



Skeletal myoblast（骨格筋芽）

Kobayashi, M., Haraguchi, Y., Shimizu, T., Mizuuchi, K., Iseki, H. (2015). Realtime, noninvasive optical coherence tomography of cross-sectional living
cell-sheets in vitro and in vivo. J Biomed Mater Res Part B: 103B, 12671273.
Kikuchi, T., Shimizu, T., Wada, M., Yamato, M., Okano, T. (2014). Automatic
fabricationof

3-dimensional

tissues

using

cell

sheet

manipulator

technique. Biomaterials 35, 2428-2435.
Shudo, Y., Miyagawa, S., Ohkura, H., Fukushima, S., Saito, A., Shiozaki, M.,
Kawaguchi, N., Matsuura, N., Shimizu, T., Okano, T., Matsuyama, A., Sawa, Y.
(2014). Addition of mesenchymal stem cells enhances the therapeutic
effects of skeletal myoblast cell-sheet transplantation in a rat ischemic
cardiomyopathy model. TISSUE ENGINEERING: Part A Volume 20, Numbers
3 and 4.
Terajima, Y., Shimizu, T., Tsuruyama, S., Sekine, H., Ishii, H., Yamazaki, K.,
Hagiwara, N., Okano, T. (2014). Autologous skeletal myoblast sheet therapy
for porcine myocardial infarction without increasing risk of arrhythmia. Cell
Medicine Vol. 6, 99-109.
Kikuchi, T., Shimizu, T., Wada, M., Yamato, M., Okano, T. (2014). Automatic
fabricationof

3-dimensional

tissues

using

cell

sheet

manipulator

technique. Biomaterials 35, 2428-2435.
Tadakuma, K., Tanaka, N., Haraguchi, Y., Higashimori, M., Kaneko, M.,
Shimizu, T., Yamato, M., Okano, T. (2013). A device for the rapidtransfer/transplantation of living cell sheets with the absence of cell
damage. Biomaterials 34, 9018-9025.
Ngo, T., Nagamori, E., Kikuchi, T., Shimizu, T., Okano, T., Taya, M., Kino-oka,
M. (2013). Endotherial cell behavior inside myoblast sheets with different
thickness. Biotechnol Lett 35, 1001-1008.
Shudo, Y., Miyagawa, S., Nakatani, S., Fukushima, S., Sakaguchi, T., Saito, A.,
Asanuma, T., Kawaguchi, N., Matsuura, N., Shimizu, T., Okano, T., Sawa, Y.
(2013).

Myocardial

layer-specificeffectofmyoblast

cell-sheet

implantation evaluated by tissue strain imaging. Circ J 77, 1063-1072.
Tadakuma, K., Tanaka, N., Haraguchi, Y., Higashimori, M., Kaneko, M.,
Shimizu, T., Yamato, M., Okano, T. (2013). A device for the rapidtransfer/transplantation of living cell sheets with the absence of cell
damage. Biomaterials 34, 9018-9025.

Nagamori, E., Ngo, T., Takezawa, Y., Saito, A., Sawa, Y., Shimizu, T., Okano,T., Taya, M., Kino-oka, M. (2012). Network formation through active
migration of human vascular endothelial cells in a multilayered skeletal
myoblast sheet. Biomaterials 34, 662-668.
Miyagawa, S., Roth, M., Saito, A., Sawa, Y. &amp; Kostin, S. (2011) Tissueengineered cardiac constructs for cardiac repair. Ann Thorac Surg, 91(1),320-329.
Miyagawa, S., Saito, A., Sakaguchi, T., Yoshikawa, Y., Yamauchi, T., Imanishi,Y., Kawaguchi, N., Teramoto, N., Matsuura, N., Iida, H., Shimizu, T., Okano, T.
&amp; Sawa, Y. (2010) Impaired myocardium regeneration with skeletal cellsheets--a preclinical trial for tissue-engineered regeneration therapy.
Transplantation, 90(4), 364-372.
Haraguchi, Y., Sekine, W., Shimizu, T., Yamato, M., Miyoshi, S., Umezawa, A.
&amp; Okano, T. (2010) Development of a new assay system for evaluating
the permeability of various substances through three-dimensional tissue.
Tissue Eng Part C Methods, 16(4), 685-692.
Hoashi, T., Matsumiya, G., Miyagawa, S., Ichikawa, H., Ueno, T., Ono, M.,
Saito, A., Shimizu, T., Okano, T., Kawaguchi, N., Matsuura, N. &amp; Sawa, Y.
(2009) Skeletal myoblast sheet transplantation improves the diastolic
function of a pressure-overloaded right heart. J Thorac Cardiovasc Surg,
138(2), 460-467.
Kondoh, H., Sawa, Y., Miyagawa, S., Sakakida-Kitagawa, S., Memon, I.A.,
Kawaguchi, N., Matsuura, N., Shimizu, T., Okano, T., and Matsuda, H. (2006).
Longer preservation of cardiac performance by sheet-shaped myoblast
implantation in dilated cardiomyopathic hamsters. Cardiovascular research69, 466-475.
Hata, H., Matsumiya, G., Miyagawa, S., Kondoh, H., Kawaguchi, N., Matsuura,N., Shimizu, T., Okano, T., Matsuda, H., and Sawa, Y. (2006). Grafted skeletal
myoblast sheets attenuate myocardial remodeling in pacing-induced
canine heart failure model. The Journal of thoracic and cardiovascularsurgery 132, 918-924.
Memon, I.A., Sawa, Y., Fukushima, N., Matsumiya, G., Miyagawa, S., Taketani,S., Sakakida, S.K., Kondoh, H., Aleshin, A.N., Shimizu, T., Okano, T. andMatsuda, H. (2005). Repair of impaired myocardium by means ofimplantation of engineered autologous myoblast sheets. The Journal ofthoracic and cardiovascular surgery 130, 1333-1341.



Smooth muscle cell（平滑筋細胞）

Talab, S., Kajbafzadeh, A., Elmi, A., Tourchi, A., Sabetkish, S., Sabetkish, N.,
Monajemzadeh, M. (2014). Bladder reconstruction using scaffold-lessautologous smooth muscle cell sheet engineering: early histologicaloutcomes for autoaugmentation cystoplasty. BJU Int 114, 937-945.
Shudo, Y., Cohen, J., MacArthur, J., Atluri, P., Hsiao, P., Yang, E., Fairman, A.,
Trubelja, A., Patel, J., Miyagawa, S., Sawa, Y., Woo, Y. (2013). Spatiallyoriented, temporally wequential smooth muscle cell-endothelial progenitor
cell bi-level cell sheet neovascularizes ischemic myocardium. Circulation
128, 59-68.
Hobo, K., Shimizu, T., Sekine, H., Shin'oka, T., Okano, T., and Kurosawa, H.
(2008). Therapeutic Angiogenesis Using Tissue Engineered Human SmoothMuscle Cell Sheets. Arteriosclerosis, thrombosis, and vascular biology 28,
637-643.

Synoviocyte（滑膜細胞）

Yokoyama, M., Sato, M., Umezawa, A., Mitani, G., Takagaki, T., Yokoyama, M.,
Kawake, T., Okada, E., Kokubo, M., Ito, N., Takaku, Y., Murai, K., Matoba, R.,
Akutsu, H., Yamato, M., Okano, T., Mochida, J. (2016). Assessment of thesafety of chondrocyte sheet implantation for cartilage regeneration.
TISSUE ENGINEERING Part C Volume 22 Number 1.
Takaku, Y., Murai, K., Ukai, T., Ito, S., Kokubo, M., Satoh, M., Kobayashi, E.,
Yamato, M., Okano, T., Takeuchi, M., Mochida, J., Sato, M. (2014). In vivo celltrackingbybioluminescenceimaging

after

transplantationofbioengineered cell sheets to the knee joint. Biomaterials 35, 2199-2206.



Tendon derived stem/progenitor cell(TSC)（腱由来幹細胞）

Komatsu, I., Wang, J., Iwasaki, K., Shimizu, T., Okano, T. (2016). The effectof tendon stem/ progenitor cell (TSC) sheet on the early tendon healing ina rat achilles tendon injury model. Acta Biomaterialia 42, 136-146.



Thyroid（甲状腺）Arauchi, A., Shimizu, T., Yamato, M., Obara, T. &amp; Okano, T. (2009)
Tissue-engineered thyroid cell sheet rescued hypothyroidism in rat models
after receiving total thyroidectomy comparing with nontransplantation
models. Tissue Eng Part A, 15(12), 3943-3949.



Urothelial cell（尿路上皮細胞）

Shiroyanagi, Y., Yamato, M., Yamazaki, Y., Toma, H., and Okano, T. (2004).
Urothelium regeneration using viable cultured urothelial cell sheets
grafted on demucosalized gastric flaps. BJU international 93, 1069-1075.
Shiroyanagi, Y., Yamato, M., Yamazaki, Y., Toma, H., and Okano, T. (2003).
Transplantableurothelialcellsheetsharvestednoninvasively

fromtemperature-responsive culture surfaces by reducing temperature. Tissue
engineering 9, 1005-1012.



Vein endothelial cells（血管内皮細胞）

Sugibayashi, K., Kumashiro, Y., Shimizu, T., Kobayashi, J., Okano, T. (2012).
A molded hyaluronic acid gel as a micro-template for blood capillaries.
Journal of Biomaterials Sciences, Polymer Edition, 24:2, 135-147.
Sekiya, S., Shimizu, T., Yamato, M. &amp; Okano, T. (2011) &quot;Deepmedia culture condition&quot; Promoted lumen formation of endothelial
cells within engineered three-dimensional tissues in vitro. J Artif Organs,14(1), 43-51.
Sekiya, S., Muraoka, M., Sasagawa, T., Shimizu, T., Yamato, M. &amp; Okano,
T. (2010) Three-dimensional cell-dense constructs containing endothelial
cell-networks are an effective tool for in vivo and in vitro vascular biology
research. Microvasc Res, 80(3), 549-551.
Asakawa, N., Shimizu, T., Tsuda, Y., Sekiya, S., Sasagawa, T., Yamato, M.,
Fukai, F. &amp; Okano, T. (2010) Pre-vascularization of in vitro threedimensional tissues created by cell sheet engineering. Biomaterials,
31(14), 3903-3909.
Ohno, M., Motojima, K., Okano, T. &amp; Taniguchi, A. (2008) Up-regulationof drug-metabolizing enzyme genes in layered co-culture of a human liver
cell line and endothelial cells. Tissue Eng Part A, 14(11), 1861-1869.



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Moschouris, K., Firoozi, N., Kang, Y. (2016). The application of cell sheetengineering in the vascularization of tissue regeneration. Regen. Med.
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Three-dimensional

cardiactissuefabrication based on cell sheet technology. Advanced Drug Delivery
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Takahashi, H., Okano, T. (2015). Cell sheet-based tissue engineering fororganizing anisotropic tissue constructs produced using microfabricatedthermoresponsive substrates. Adv. Healthcare Mater. 4, 2388-2407.
Egami, M., Haraguchi, Y., Shimizu, T., Yamato, M., Okano, T. (2014). Latest
status of the clinical and industrial applications of cell sheet engineeringand regenerative medicine. Arch. Pharm. Res. 37, 96-106.
Sato, M., Yamato, M., Hamahashi, K., Okano, T., Mochida, J. (2014). Articularcartilage regeneration using cell sheet technology. THE ANATOMICALRECORD 297, 36-43.
Iwata, T., Yamato, M., Ishikawa, I., Ando, T., Okano, T. (2014). Tissue
engineering in periodontal tissue. THE ANATOMICAL RECORD 297, 16-25.
Okano, T., Dezawa, M. (2014). A New age of regenerative medicine: fusionof tissue engineering and stem cell research. THE ANATOMICAL RECORD
297, 4-5.
Owaki, T., Shimizu, T., Yamato, M., Okano, T. (2014). Cell sheet engineering
for regenerative medicine: current challenges and strategies. Biotechnol.
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Kobayashi, J., Okano, T. (2013). Thermoresponsive thin hydrogel-grafted
surfaces for biomedical applications. Reactive &amp; Functional Polymers,
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Sakai, Y., Koike, M., Hasegawa, H., Yamanouchi, K., Soyama, A., Takatsuki,M., Kuroki, T., Ohashi, K., Okano, T., Eguchi, S. (2013). Rapid fabricating
technique for multi-layered human hepatic cell sheets by forcefulcontraction of the fibroblast monolayer. PLoS ONE 8(7):e70970.
Matsuura, K., Haraguchi, Y., Shimizu, T., Okano, T. (2013). Cell sheettransplantation for heart tissue repair. Journal of Controlled Release 169,
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Arisaka, Y., Kobayashi, J., Yamato, M., Akiyama, Y., Okano, T. (2013).
Heparin-functionalized thermoresponsive surface. Organogenesis 9:3, 1-3.
Umemoto, T., Yamato, M., Nishida, K., Okano, T. (2012). Regenerative
medicine of cornea by cell sheet engineering using temperatureresponsive culture surfaces. Chin Sci Bull.
Dutta, R. C. &amp; Dutta, A. K. (2010) Comprehension of ecm-cell dynamics:
A prerequisite for tissue regeneration. Biotechnol Adv, 28(6), 764-769.
Dutta, R. C. &amp; Dutta, A. K. (2010) Comprehension of ecm-cell dynamics:
A prerequisite for tissue regeneration. Biotechnol Adv, 28(6), 764-769.
Elloumi-Hannachi, I., Yamato, M., and Okano, T. (2010) Cell sheetengineering:auniquenanotechonology

forscaffold-freeTissuereconstruction with clinical applications in regenerative medicine. J lnten
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highfunctionalizationoftemperature-responsive culture dishes for establishing advanced cellsheet engineering. J Mater Chem, 20(40), 8768-8775.
Yang, J., Yamato, M., Shimizu, T., Sekine, H., Ohashi, K., Kanzaki, M., Ohki, T.,
Nishida, K., and Okano, T. (2007). Reconstruction of functional tissues withcell sheet engineering. Biomaterials 28, 5033-5043.
Yang, J., Yamato, M., Nishida, K., Ohki, T., Kanzaki, M., Sekine, H., Shimizu,T., and Okano, T. (2006). Cell delivery in regenerative medicine: the cellsheet engineering approach. Journal of controlled release 116, 193-203.
Yang, J., Yamato, M., Nishida, K., Hayashida, Y., Shimizu, T., Kikuchi, A., Tano,Y., and Okano, T. (2006). Corneal epithelial stem cell delivery using cellsheet engineering: not lost in transplantation. Journal of drug targeting 14,
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RepCell®

Wang, X., Hisha, H., Mizokami, T., Cui, W., Cui, Y., Shi, A., Song, C., Okazaki,
S., Li, Q., Feng, W., Kato, J. &amp; Ikehara, S. (2010) Mouse mesenchymal
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Guo, Y., Zhao, G., Tanaka, S. &amp; Yamaguchi, T. (2009) Differential
responses between monocytes and monocyte-derived macrophages forlipopolysaccharide stimulation of calves. Cell Mol Immunol, 6(3), 223-229.
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McNulty, A.K., Schmidt, M., Feeley, T., and Kieswetter, K. (2007). Effects ofnegative pressure wound therapy on fibroblast viability, chemotactic
signaling, and proliferation in a provisional wound (fibrin) matrix. Wound
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Kupffer cells. J Immunol Methods 326, 139-144.
Gordon, I.O., and Freedman, R.S. (2006). Defective antitumor function of
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Clin Cancer Res 12, 1515-1524.



HydroCell®

Kojima, N., Kato, C., Igarashi, M., and Ishii, M.(2011) Development ofperitoneal macropharge along a dendritic cell lineage in response to uptakeof oligomannose-coated liposomes. Cell Immunol In Press, AcceptedManuscript
Yoshida, Y., Tsunoda, T., Doi, K., Tanaka, Y., Fujimoto, T., Machida, T., Ota,
T., Koyanagi, M., Takashima, Y. &amp; Sasazuki, T. (2011) Kras-mediated upregulation of rrm2 expression is essential for the proliferation of colorectal
cancer cell lines. Anticancer Res, 31(7), 2535-2539.
Tan, S. L., Sulaiman, S., Pingguan-Murphy, B., Selvaratnam, L., Tai, C. C.
&amp; Kamarul, T. (2011) Human amnion as a novel cell delivery vehicle forchondrogenic mesenchymal stem cells. Cell Tissue Bank, 12(1), 59-70.
Ito, K., Nakamura, H. &amp; Watanabe, Y. (2011) Protogenin mediates celladhesion for ingression and re-epithelialization of paraxial mesodermal
cells. Dev Biol, 351(1), 13-24.
Ishii, M., Kato, C., Hakamata, A. &amp; Kojima, N. (2011) Targeting witholigomannose-coatedliposomes

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trafficking of dendritic cells in the peritoneal cavity. Int Immunopharmacol,
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Yanase, Y., Hiragun, T., Kaneko, S., Gould, H. J., Greaves, M. W. &amp; Hide,M. (2010) Detection of refractive index changes in individual living cells bymeans of surface plasmon resonance imaging. Biosens Bioelectron, 26(2),674-681.
Yanase, Y., Araki, A., Suzuki, H., Tsutsui, T., Kimura, T., Okamoto, K.,
Nakatani, T., Hiragun, T. &amp; Hide, M. (2010) Development of an opticalfiber spr sensor for living cell activation. Biosens Bioelectron, 25(5), 12441247.
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Notani, T., Tabata, M. J., Iseki, H., Baba, O. &amp; Takano, Y. (2009)
Introduction of a three-dimensional and layered (tdl) culture, a novel
primary co-culture method for ameloblasts and pulp-derived cells. Archivesof Histology and Cytology, 72(3), 187-198.
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