Dr. Christian JurschEurovir Hygiene-Labor GmbHIm Biotechnologiepark TGZ I
D-14943 LuckenwaldeEurovir®Hygiene-Labor
Antivirale Validierung & Rabies

Testing of the virucidal activity of the printer vernish„Lock 3 WB OPV“against Vacciniavirus
Screening test of the product using the quantitative carrier test
following the RKI-Richtlinie (1995) against the Modified Vacciniavirus Ankara (MVA)- Excerpt from the test report: screening test S7 dated 07.12.2020 -byPD Dr. Olaf Thraenhart and Dr. Christian Jursch

Study time:
Principal:

November - December 2020
dyphox® Hygiene Solutions
TriOptoTec GmbHAm Biopark 13D-93053 Regensburg / Germany

Eurovir Hygiene-Labor GmbHIm Biotechnologiepark TGZ I
D-14943 Luckenwalde
Geschäftsführer: Dr. Christian Jursch
Hauptgesellschafter: PD Dr. Olaf Thraenhart

Amtsgericht Potsdam
Handelsregister-Nr.: HRB 26128 P
Steuer-Nr.: 050/108/05610
USt-IdNr.: DE 288 863 508

MittelbrandenburgischeSparkasse in Potsdam
SWIFT/BIC: WELA DE D1 PMB
IBAN: DE14 1605 0000 1000 9939 37

Virucidal activity of the „Siegwerk Drucklack“ vs. MVA-Vacciniavirus - Excerpt from the test report S7 dated 07.12.2020 - page 1 (of 2)

Dr. Christian JurschEurovir Hygiene-Labor GmbHIm Biotechnologiepark TGZ I
D-14943 Luckenwalde

Eurovir®Hygiene-Labor
Antivirale Validierung & Rabies

Aim of the testing and performing the test
The printer varnish Lock 3 WB OPV should be tested for its ability to inactivate the Vacciniavirus 1 under the influence of light.
To test this feature, test squares (PVC-foil) were coated with the printer varnish Lock 3 WB
OPV. Afterwards the test virus material, containing the Modified Vacciniavirus Ankara (MVA)
were evenly distributed on the surface of the coated test specimen and exposed to the irra diation with visible light. After irradiation the virus material was then recovered from thetest carriers and the remaining amount of virus was quantified.
The underlying test was carried out in the dry state based on the guideline of the Robert
Koch-Institute (1995) and ISO 21702 (modified) at room temperature and under the influ ence of visible light.
Test results
The testing of the printer varnish Lock 3 WB OPV under the described test conditions andwith the Modified Vacciniavirus Ankara (MVA) as the test virus has shown that:1.

with the printer varnish Lock 3 WB OPV and after irradiation with visible light a sig nificant reduction of the test virus was recorded. The virus reduction on the test sur face amounted to more than 6,85 Log, corresponding to a virus inactivation of morethan 99,99%.

2.

without irradiation with light, the test samples have shown no virus-inactivatingactivity.

Judgement
On the basis of the data obtained it can therefore be concluded that the described antiviraleffect on the Modified Vacciniavirus Ankara (MVA) can clearly be attributed to the photodynamic effect of the printer varnish under test.
Luckenwalde, 07th of December 2020

Dr. Ch. Jursch(GF and Laboratory Manager Eurovir)_______________________________________________________________________________________________________________1= Annotation regarding virucidal activity: according to EN14476 the Vaccinivirus is the (only) test virus for the so-called„limited virucidal activity“. This efficacy claim includes an efficacy against the enveloped viruses (e.g. HIV, HCV, HBV, the influenza viruses as well as SARS-CoV-1 and and SARS-CoV-2 [and others]).

Virucidal activity of the „Siegwerk Drucklack“ vs. MVA-Vacciniavirus - Excerpt from the test report S7 dated 07.12.2020 - page 2 (of 2)